LOCUS       DAOSPM010000001       101654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_183_length_101654_cov_12.730391, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000001 DAOSPM010000000
VERSION     DAOSPM010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 101654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 101654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101654
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183_length_101654_cov_12.730391"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..889
                     /locus_tag="PLW25_00005"
     CDS             155..889
                     /locus_tag="PLW25_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOS52899.1"
                     /translation="MNQKENPQSVIDKYSKRQKYMPYVLGGLAGILALAGIFIIISVA
                     TGSGNLFKGMFATKTPTPTATFTPTATVPSPTPSMTPTITETPTPAATDTPSGPQFYT
                     VKQGDDCWTLANTFGVDLLVLLAINNFPSGTCPLVPGTDIIIPAPGQTLPTSTPLPAD
                     IPRGTEIEYTIQLGETLQDIASRFNSTLEDIKTRNNIKNENLIYVGQVIKVRVNLVTA
                     TPTVAPTSTLSLFTLQPTKPAVTTTP"
     gene            complement(954..2009)
                     /gene="queG"
                     /locus_tag="PLW25_00010"
     CDS             complement(954..2009)
                     /gene="queG"
                     /locus_tag="PLW25_00010"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOS52900.1"
                     /translation="MSQIPSSLESEIKARALSLGFTVCGITTPDTLDFHHMYVNWLER
                     GLNAGMTYLASAYHVSNRLHPEKFFPGLKSIILLGYRYPLQHPAEIEEKTTGLISGYA
                     SGKDYHHLIPNLASPLLQYLTALNPEAPAPRVYTDSAPVLERELAVRSGLGWIGRNSC
                     LISPKHGSNLLLAEIFTAIALQADPPFAADHCGSCMRCVESCPTGCILPERVIDANRC
                     LSYHNIENKGMIPPEVMEKFGGWIFGCEICQMVCPWNQRGYQSSSSIGIAGQMTVEEM
                     LEFLELSMDDFKSRFAETALARARYNGLKRNVIIRLANLGEKNALGSIKRTEDTAELD
                     WLRDTARWARNKIQLKK"
     gene            complement(2027..2734)
                     /locus_tag="PLW25_00015"
     CDS             complement(2027..2734)
                     /locus_tag="PLW25_00015"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOS52901.1"
                     /translation="MLKRRMLFNFIIVIVIGMLLVSCKGKPSIDPNQKMTEVAATVQA
                     GLTQAAASNPTATPTLEPTATATPSPQTPTPLTAISSPVTVLVPTQPQTSTADNALFI
                     TDVTYPDGSVVKPGEHIVKTWKLQNTGKTTWTKEYSVLYLEGVLYGAGSQLMFRLPAE
                     VKPGDFAEISVPFIAPTTPGNYGSFWKMYNSSGYVFGDPFNISIIVGEPTPTGNAPTA
                     TITPTITGTVSAGWGTP"
     assembly_gap    2806..2905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2906..3573)
                     /locus_tag="PLW25_00020"
     CDS             complement(<2906..3573)
                     /locus_tag="PLW25_00020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOS52902.1"
                     /translation="MKKPSGIPQRWAVIGFIISVFFISAAAPVNNLPFSTNVNSSSQG
                     DALRLGDTPEDPYYPSNKPFFQAPLSLTPHDHFWLSRPIRDEILTWRTADYRYGYFIK
                     QESIVHGGLDIPAPEKTPVLAAADGTVVFSGYGLLYGEGARNDPYGIAVMVRHDMELD
                     GDTLYTVYTHLGKTLVKVNELVKAGQKIGAVGMTGNTSGPHLHFEVRVKGKNGMLLQN
                     PELW"
     gene            complement(3570..>4528)
                     /locus_tag="PLW25_00025"
     CDS             complement(3570..>4528)
                     /locus_tag="PLW25_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52903.1"
                     /translation="RTDEFEEIEAWNLESEQTRPVTMGSGSHPVWMDEEQGVLAVVHS
                     ANSNDLVAYSTEPNLLLLPPIHQKNEVLSISWLPAKSALPLKKYLSQHSPASETRLWQ
                     PEISTQDVSSGRYEMVYLPDVTAPEPYLIDTVNESFNSMRAALRSKLGWDFLKILESA
                     SISIPAYSSSGLREDWGYTGRAITVNLDPLDSGWMAVSREDILGRTYWRVWLKCMQQD
                     GTCGVPIQNPVWDFNARIQGSSSAYENGGLATTLPQGYWIDFTSFALGYGWERLPAGS
                     NWQVYFPETRINRFVLRDGLSWHEAMRQRFTDTTIESYWSLP"
     assembly_gap    4529..4628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4629..5479)
                     /locus_tag="PLW25_00030"
     CDS             complement(<4629..5479)
                     /locus_tag="PLW25_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52904.1"
                     /translation="MNTSARLCTRLILLIPFLVVSLSACKPEPAAPEIAAHVEGTVVS
                     LPEPSETASPLLSTPTLIPTTSPGSVATSENPATSGLIILAVKIGGFAQLAAYHPLNQ
                     ALTQITDDEWDHADPAISPDGTKLAFCADEFGHWDIFILDLPTGEKTNLTNATTYACS
                     PTWSPDGRWVAYEVVMDGKLNLVIRSVMDESVAPHRLTDNGGNNFDPAWSPSGREIAF
                     VTDRNGRLELWLANLDNPQQRFNLVARSEHADYTHPVWSGDGHSLAWVRTDEFEEIEA
                     WNLESEQT"
     gene            complement(5523..6401)
                     /locus_tag="PLW25_00035"
     CDS             complement(5523..6401)
                     /locus_tag="PLW25_00035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOS52905.1"
                     /translation="MKKQRAVILCLTLSFVLVATGCSSSFPQPPTPTPVMTTPTFHAT
                     TTPSPSHTATQNPTPAFTATLQPAIKACSPLKDIELSDLHKIMSQGFTPPAPYKDDGH
                     PAIDLAFFTFKELPSMIGHPVQSIFPGIVNLVIVDRFPYGNAVLIETPLNVLSAEWVS
                     ALPIPTAIPQENLDLVRPCDSDPLFADMTPIEWDGSRKSVYVLYAHLLEKPSVRAGDV
                     VNCGQVIGAAGNTGNSAAEHLHLEARVGPAGAQFGTLAMYSPDATIEERYRYCIWSSS
                     GRFQSFNPVILWEQEP"
     gene            complement(6411..6746)
                     /locus_tag="PLW25_00040"
     CDS             complement(6411..6746)
                     /locus_tag="PLW25_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52906.1"
                     /translation="MNTPHNSPKVTAPPQIDRLQAKLLPDNQRVRVTLVLNNVECRPT
                     LELSLLDEKQMEIARSTIIGTFNPLVSFTLHLGQHSPNDRLFLQAVVFPNDNEFSDSK
                     KVPVEVGSQ"
     gene            complement(6773..8092)
                     /locus_tag="PLW25_00045"
     CDS             complement(6773..8092)
                     /locus_tag="PLW25_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOS52907.1"
                     /translation="MRNYPEIGVQVPEIYLPVEKIDLTKWAVIACDQYTSQPEYWEQV
                     AKNVGDCPSTFHLILPEVYLGTDKETERVQSTHAKMQQYLSDGLLRPQQNIIYVERTI
                     GVKTRRGLMLALDLEHYDFSKGSQSLIRATEGTILDRLPPRIRIRKNAPLELPHIVVL
                     IDDPEDRVIGPVVQASQRLPLAYDFDLMQRGGHLRSRLVNDSALEKSIIQNLAALANP
                     QTFYAKYRVSPDKGVLLFAVGDGNHSLATAKAIWEELKSSVSMDHPARFALVEVENIH
                     DAGLAFEPIHRVLFNSKVNLEQVLKGIYDGALTLEPVSSFTELTTKVKQPSVECQRFG
                     LVSTRGLVAGSITSPTANLVVGTLQTILDQLVADGSVASIDYVHGDEALCELGMKPAN
                     TGIYLPGMSKDELFRTVMLDGALPRKTFSMGEAHEKRFYMESRKITI"
     gene            complement(8189..9049)
                     /locus_tag="PLW25_00050"
     CDS             complement(8189..9049)
                     /locus_tag="PLW25_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOS52908.1"
                     /translation="MNKVMVVTDSTANIPPELWDGNPIVSVPLQVIWGNEILRDGVDI
                     QAKEFYERLAVADVMPSTSQVTPEEFKAMYGRLIEEGYDILSIHISSKLSGTLDSAIQ
                     AKNAFSGARIELVDSLSTSMAMGFQVLSAARMAGIGATLEECKAVAEKAKANTGVYFV
                     LNTLEFLHRGGRIGGAQAFLGTMLNMKPILELRDGRIEAVERVRTIGKATERLLDLVE
                     QRVGTAQGPVRLCVVHANAEKEGQALLERAIKRFPSSLVTDAVLSTVSPVLGTHAGPG
                     ALGVAFMHSM"
     gene            9231..10553
                     /gene="ffh"
                     /locus_tag="PLW25_00055"
     CDS             9231..10553
                     /gene="ffh"
                     /locus_tag="PLW25_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOS52909.1"
                     /translation="MFENLTGRLNQIFTQLRRKGKLSAADVDAMMREVRLALLEADVN
                     YTVVKDFTTRVRERAIGAEVSKALNPAQQVIKIVHEELIHTLGEPARLNLSGVKPYAI
                     MLVGLQGSGKTTAAAKLAKRLRSDGERVLLVAGDPYRPAAVKQLQTLGEKIGVEVVAD
                     STLKPPALAAHAFDKAQKGGYSVVIIDTAGRSQLDQTLMDELQSIAKKVRLVETLLVV
                     DAMIGQEALHVAEGFRDTVNITGLVFTKIDGDARGGAAISVRKVTGVPIKFLGTGEGI
                     DAVEVFDPARLASRILGMGDMIGLIEKAEAAFDEKTQKEQAERVLKGQFNLEDFADQL
                     KQLRKMGPISQVMDMLPGGLGQASRNIPPEETERNLKRTEAIIASMTKQEKRDPDLLN
                     ASRRRRIASGSGTNVMEVNRLIKQFRDVQRLMKTMQKTGGKGLSRLFK"
     gene            10661..10996
                     /gene="rpsP"
                     /locus_tag="PLW25_00060"
     CDS             10661..10996
                     /gene="rpsP"
                     /locus_tag="PLW25_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOS52910.1"
                     /translation="MVRIRLRRTGQRHQPSYRIIVADKESPRDGRFLEIIGHYIPTTD
                     PFTFEVQEDRVYEWMKNGALPSDSVLKLFKIVGVLERYERFKKGESLETLLAEAKKAT
                     EERNAKIKA"
     gene            11005..11304
                     /locus_tag="PLW25_00065"
     CDS             11005..11304
                     /locus_tag="PLW25_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOS52911.1"
                     /translation="MMQPTNRPTQRYSRNVNQESHIKELVEYIALSLVDDPSQVVVTE
                     YRSGGRENLELHVAREDMGRVIGKGGKIANAIRVLLRVAAAREGKQVNLDVAEPR"
     gene            11301..11861
                     /gene="rimM"
                     /locus_tag="PLW25_00070"
     CDS             11301..11861
                     /gene="rimM"
                     /locus_tag="PLW25_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOS52912.1"
                     /translation="MTNRKSTGPQLKTQPGSPQPGEPVYLMIGKLRRPHGVKGELWLE
                     LTTEHPERIRTGVTILIGDEKTEVKVENIRAADKLWLISFAGYQDCQEVEGFRNQWVF
                     IEAKQAAPLPAGRYYHHEIIGMKVFTETHDLIGVVDEILVTGANDVYVVKNDKGTETL
                     VPAIKSVILEIDKEMGRMIVRQQEWL"
     gene            11871..12983
                     /gene="dprA"
                     /locus_tag="PLW25_00075"
     CDS             11871..12983
                     /gene="dprA"
                     /locus_tag="PLW25_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOS52913.1"
                     /translation="MEPDKYWVGFNLVKGIGAVRFKQVLDFFGSAETAWFAPVSGLQA
                     AGLPQRVIENFLLVKSQVDLERVMERIHHSGTRVITWNDQDYPRRLKEIDQSPPVLFI
                     RGSINVEDDWAVAVVGTRRVTPYGRQVANEIATFLGQNGVTVVSGLARGVDAIAHQSA
                     LAAGGRTIAVLGSGVDVIYPPEHRKLAVEIIAQGALVSDYPLGTSPDGVNFPPRNRII
                     SGLSLATVVVEAGEKSGALITAEFAVEQGRDVFAVPGSILAPQSAGTNQLIEQGARPL
                     LKMAEILEVLKLEQIPEKQQARKLNPLNQAEQNLLQHLSTEPLHIDQLCELTGLPIHD
                     VSATLSMMELKGFICQVGGMNYVAMRENRGLYKVNK"
     gene            12980..14074
                     /locus_tag="PLW25_00080"
     CDS             12980..14074
                     /locus_tag="PLW25_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOS52914.1"
                     /translation="MKIEHYYAVIMAGGGGTRLWPLSRHDSPKQTLPIFEGKSLFQLA
                     VERLQGYLPVNQIYVVTSADLMEQLHAQVPELLTSNFLIEPQPRGTAAVVALAAAAIL
                     ERDPNGVLAVLTADHLMPDKAEFQACLQAAAELAEQGYIVTMGIKPASPATGYGYIER
                     GAELGVYSGRVGYEVLKFIEKPHQSKANELIKQERYSWNSGMFICQAQVVLGEFERYM
                     PELYTVIQSLQPFLASDHLAAPFAVKWLKIQPETIDYGIMEKTSLAAVLPLSTLEWID
                     VGSWDSFFEVFKPDENGNIILGVKYEGIDTADSLILSTDTERPVVTMGMKNMIVIQAP
                     DVLMICPRGESQRVKELVNYLKASDYTLIL"
     gene            14086..16389
                     /gene="topA"
                     /locus_tag="PLW25_00085"
     CDS             14086..16389
                     /gene="topA"
                     /locus_tag="PLW25_00085"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOS52915.1"
                     /translation="MEAYCVKCKQKREIESPNAEFNALGTPVTRGKCPVCNTSLYRIG
                     ETPAHAGLERPKRLHAKPRSGKLVIVESPAKAKTVGRFLGKDYTVRASVGHVRDLLRS
                     ELSVDVENNFLPKYRVPNEKRPVVKELKALAKEAAEIYLATDPDREGEAIAWHLLEAA
                     QIEPKLSRRVVFHEITDHAINEAFSQPREINMDLVDAQQARRILDRLVGYSISPVLWE
                     KVRSRLSAGRVQSVALRLIVEREREIEVFVPVEYWSIHAELTPEGKKTSYMTKLAKID
                     EVDPDLGNKEVVDGYLADIEASPFAVTRIKKSERRRKPSAPFITSTLQQEASRRLGYT
                     ARRTMMIAQQLYEGLEVGEGGATGLITYMRTDSTNVAEVAQEEARSFIQQTYGAGFLP
                     EKPPQYKTRAVSAQEAHEAIRPTSVLRQPEKIKEFLSSEQSKLYQLIWQRFVASQMES
                     AVYDTISVEVTATGKDHQYLFRASGSTIKFPGFLIVYEETVDEDLKTEETENVRIPAG
                     LVEGQAQHLKRLIPEQHFTQPPPRYTEASLVQVLEEYGIGRPSTYAPILSTIQQRGYV
                     LREAKRLSPTETGILVNDLVVEHFPDIVDLNFTAGMEAHLDQVAAGKEDWHKVLADFY
                     TTFEPQVKRAQAEMPVTKTEPEKIGRACPNCGHDLVIRWGRYGKFISCSNFPACRHTE
                     ALLEKIGVTCPKDDGDVVLRKTRKGRIFFACSNYPNCDFTSWKRPLPVPCPKCGGWMV
                     VANKREAQCMVCEESVLIEELGVPIPD"
     gene            16453..17766
                     /locus_tag="PLW25_00090"
     CDS             16453..17766
                     /locus_tag="PLW25_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52916.1"
                     /translation="MDKLRDVFFKLIDKPLYTAIAGFVIGLIIGLPILGWWLWPVKWK
                     DADASFLRQDLKNQYLCMVVDSYSVNHDAALAGARINSLGVDIKKNPFLFDNLAAGGC
                     NYAPTDKVIQSLKTALVTGEQVVPETEANNQPDGDQAEIKATEPLATAEIAGSTAIPL
                     PGNNAPKEKKGGSVLLLSLLCVVFLLIGGALIYIFFIRNRRTSEEIPHAWDEEERFAQ
                     DEPYDDAPEYPAAATPPPPTAIHHESVDTPPMDYFSEPEEMDDVDEMEIPDILEGAQD
                     VAKTPPLKSPGVRATLPHHSDPVAHFMTTYVIGDDLYDDSFSIDAPNGSFLGECGVGI
                     LDTIGVGDPKKVTAFEVWLFDKNDIQTVTKVLLSAHAMNDAGVKQRLASKGELVQIES
                     GQQLLLETATLQLEARVVEMVYGQGALPASSFFQRMTLELSVWPK"
     gene            complement(17810..18277)
                     /gene="greA"
                     /locus_tag="PLW25_00095"
     CDS             complement(17810..18277)
                     /gene="greA"
                     /locus_tag="PLW25_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOS52917.1"
                     /translation="MNQPSYLTREGAEKLRAELKHLEGPIRQELAKRLRAAIQMGDLS
                     ENADYAAAKEEQAFVEGRIQELTQILRDVVLIEDGPSHTGKIQIGSTVTIQEDQEEEE
                     TFHIVGPQEADPRRGKISYDSPIGQALLGHQEGDRITANTPAGELHFRILKVK"
     gene            complement(18305..19456)
                     /locus_tag="PLW25_00100"
     CDS             complement(18305..19456)
                     /locus_tag="PLW25_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOS52918.1"
                     /translation="MKFDRAYLEKIEEETLATYAVHASRSKGRKYPENEPLYRTCFQR
                     DRDRVLHTTSFRRLEYKTQVFINYEGDYYRTRLTHTLEVAQIGRTIARAMGANEDLVE
                     TICMAHDLGHPPFGHSGETTLNQLMEGYGGFNHNHQSYRIVTELEHRYAQFPGLNLSW
                     EVLEGIVKHETEYDKTDALDYDPHLRGHLEAQITNVADELAYTAHDLDDGLRSGMITT
                     DMLSEIDLWNMVMERLNIHSRQLDDLTRHSIIRELIGLEVDSMISESNERLQRSGVKT
                     LADLQSHAQNVVGFDDVMRARNRELKDFLYKNMYRHYRVVRMQKKAERILSELFHAYV
                     SEPNMLPIHFQDQLEIKGKEKTICDYLAGMTDRFAVDEYQKLFDPSLLP"
     gene            complement(19461..20273)
                     /locus_tag="PLW25_00105"
     CDS             complement(19461..20273)
                     /locus_tag="PLW25_00105"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HOS52919.1"
                     /translation="MTTPLFFLKLGGSLITNKNIPHQARSDVLKRLADEIARALTDQP
                     GVRLLLGHGSGSFGHIPAKKYQTRDGVATFAQWRGLVEVWHEARALNTIVMEVLHESG
                     VHAMAFPPSAQVITSSHKITHWDTTQIAACLDNGILPVVYGDVVFDQTIGGTILSTEE
                     QFEFLAAIFKPARVLLAGIEKGVWMDFPKRKELLDAITPRNLAMVDPHLESSESPDVT
                     GGMRSKVHNMMNLVTNLHCQQVLIFSGLDEGNVFQALTGRKVGTCITLDKKG"
     gene            complement(20273..21229)
                     /gene="mvk"
                     /locus_tag="PLW25_00110"
     CDS             complement(20273..21229)
                     /gene="mvk"
                     /locus_tag="PLW25_00110"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOS52920.1"
                     /translation="MPAITSSSPGKVILCGEHAVVYGAPAIALPVFQLNTKTTILAQP
                     TAPEGKVRMIAPCIGLDSDLESLAYNHPLKATTALVMQKMGVTRLQACEIRINTTIPI
                     ATGLGSSASVTVSLARALSAFLGSPLSDELVNQVAFEIEKLHHGTPSGIDNTVVTYQV
                     PVYFVKGKPIEHLKIRQPFTLVIADSGAASLTAETVARVRERWLAEKKRYDAIFANIA
                     DLSREVKENLEKNDLNQHGELLSRNHELLQALGVSTPMLDDLVNCALQAGALGAKLSG
                     GGGGGNIIALVHHTQADSISRALEQQGAVRTITTTLAPGRGA"
     gene            complement(21237..22109)
                     /locus_tag="PLW25_00115"
     CDS             complement(21237..22109)
                     /locus_tag="PLW25_00115"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOS52921.1"
                     /translation="MLKYILKAMRPRQWTKNAFIFAALVFDRKLLNLHAFLQTLAAFV
                     LFCLLSSSVYLINDILDRESDRAHPIKKNRPIASGNLPVGLALATGIFLLLVSLTGAF
                     LLSHGFFFISLVYFTLNLIYSIWLKHIPIIDVLVIASCFVLRVAAGVSVIEVQRFSPW
                     LYVVTTLLALYLGFGKRRAELAVLVNDSPQSHRKVLSGYSIQFIDQLITVVSATTLIS
                     YSLYTFSAPNLPENHSMMLTIPFVLYGIFRYLFLINKHNAGGEPEEILLKDRPIQLTV
                     LLWGVAVMVIFYLF"
     gene            22156..22995
                     /locus_tag="PLW25_00120"
     CDS             22156..22995
                     /locus_tag="PLW25_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOS52922.1"
                     /translation="MQKTRLDILMESRGLSPSRSQAQRLIMAGSVRVNHQVVLKPGTK
                     VDPGDTIEISESPRFVSRGGEKLLSALQAFHLTSLADRVCVDVGASTGGFTDCLLQHG
                     AKKVFAVDVGYGVLDWKIRNDTRVVVMEKQNARYITTFHEPIDLVVIDASFISLRTLL
                     PVVRQWLNAPKGEVVALIKPQFEAGRSETARGSGVIRDPEIHRAVLRQVLEFAQKEGY
                     TAEGLIRSPLTGPKGNIEFLAHLIYPGETPADVERLIEGLPWNEGTTADQPCQPEPEG
                     KLK"
     gene            23014..24813
                     /gene="lepA"
                     /locus_tag="PLW25_00125"
     CDS             23014..24813
                     /gene="lepA"
                     /locus_tag="PLW25_00125"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOS52923.1"
                     /translation="MSTERIRNFCIIAHVDHGKSTLADRMLQLTGTISDREMTEQVLD
                     SMDLEREKGVTIKASAVRMNYVHAGETYELNLIDTPGHVDFNYEVSRALEACEGALLV
                     VDATQGIEAQTLANLYLAIESNLTILPVINKIDLVSARPDEVAEDIESLLGLPAEEII
                     RVSAKDGLNVDQVLDAIVTQIPPPKGDDTAALQALIFDSHYDSYKGVVAYVRVMQGII
                     NADTTLRLMATKADVKPVEIGVFAPGMIPVPLLSAGDVGYVATGLKTVKECRVGDTFT
                     LSSEPAAAPLPGYKHPKPMVFAGIYPVEGEDYEALKEALERLQLNDASLVFEPETSQA
                     LNFGFRCGFLGLFHMEIIQERIEREYNLNIVVTAPSVEYEVVEISGEVLRISSPARLP
                     DEALIAEVREPWMRLEVISPTEFYGVIMELTKKRRGIFVSQDYPSPNRVQLNYDIPLS
                     ELIVDFFDDLKSRTRGYASMDYHFLDYRPGNLVKLEILVDSEPVDALAAIVHKEDAYH
                     KGQALITRLKALIPRQLFDVAIQASASGRVISRANVKALRKDVLAKCYGGDITRKKKL
                     LEKQRKGKRRMKMIGDVEIPQEAFMAVLRLEDE"
     gene            24810..25832
                     /locus_tag="PLW25_00130"
     CDS             24810..25832
                     /locus_tag="PLW25_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOS52924.1"
                     /translation="MSMVRPNWKKQAARWLPGLLISAMAVYAIAHFTRGQHIGEALKT
                     VKIGYVLLLIFLTLLSLIARSIAWRAILGNKITLVNSFFGICEGYFLNNIFPLRVGEL
                     GRALFVGRSSGLGTLHVLSTILIERAFDIVFAASIILLTLPMVVGSEWIKPIAFSAFV
                     VILVGLVILFLISVKRQKFQTWIESKEVRIGFIKNRVLPQVSKILDGLSALVHPGQFL
                     LSVFWIGITWLLWVLLYYWTIAQLAPSAPLWWGAFTSGLIALGVAIPSAPASVGVYEA
                     TILGAFALLGISSGSAVAYAIVLHLVQIFVTAVFGLWGMARDGQKLSTLLDSLVNKKN
                     VENPTG"
     gene            25840..26865
                     /locus_tag="PLW25_00135"
     CDS             25840..26865
                     /locus_tag="PLW25_00135"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOS52925.1"
                     /translation="MNGFTLITGGAGFIGANYAARCLARGEKVVIFDNLSRRGAELNI
                     EWLSDRFERRSFELVKEDVRDAAAIKTAARGADRILHLAGQVAVTTSVCDPRTDFEIN
                     AGGTFNVLEAARASGRNPVFLYASTNKVYGGMEDEQVIEQETRLDYLDHPHGINENQA
                     LDFHSPYGCSKGAGDQYTRDYFRIYGLPTVVFRQSCIYGSRQFGIEDQGWLAWFIIAA
                     VTGKPITIYGNGKQVRDVLFIDDLLDAYDAAFSRVEASRGQVFNIGGGRENSISVWLE
                     FRPILEELLGHSIKTRNREWRPGDQPVYISDIRKARSVLGWEPRVTVKQGIRRLFDWV
                     KENQNLF"
     gene            26862..28022
                     /locus_tag="PLW25_00140"
     CDS             26862..28022
                     /locus_tag="PLW25_00140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS52926.1"
                     /translation="MKILIVLTYYRPHISGLTIYAERIAKAFARRGHQVTVLTSRFEK
                     NLAADELDEDVRIIRAPVLMRLSKGVIMPTFGWLASKLVKEHDVIQLHLPQFDAAGVA
                     LRGRLLKKPTIITYHCDLELPPGLFNRAANLAVLTMNELAAKFTHRIVTYTEDYARHS
                     WYTRRYMKKVKIIPPPVVLPRVSKTEISAFQAAYNPQAQRPVIGMAARFATEKGVEVL
                     LRAIPRVINEFPNALFQFVGPYKNIIGEEGYFARLEPQIKEFEQAGNWKFLGSMSPAQ
                     MAAFYANLDLLVVPSLNSTESFGLVQIEAMMNGVRSITSDLPGVRQPVKVHRMGSIFP
                     IGDADALARGILHALQNPQLIQEANTDFSAYDPDTTAAAYERLFNEITVELH"
     gene            28053..33341
                     /locus_tag="PLW25_00145"
     CDS             28053..33341
                     /locus_tag="PLW25_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOS52927.1"
                     /translation="MVTDQPEKKKRANNGIRGNLVWDLLLIVVLLAGAIFRFTGIQWD
                     GNQHLHPDERFLTMVETGIASVKSVSDYFDTATSSLNPNNRGYTFYVYGTLPLFIVRY
                     AGEWVGKSGYDQINVVGRVLSGVFDLGTILFIYLIARRLYRHARLGLLAAGFSALAVL
                     QIQLSHYFTVDNFANFFTYGALLTAVHIATSRTFSPVDKLKEKRWKNAPDWLTTGWRS
                     AWLYALFGLLYGMALASKVSIWALAALLPLAAWIYYKNLHEEEKAAALPVLIRNLALA
                     GVIAFVSFRVFQPYAFSGPGFFGLKLNPNWLSNMRELSTLMKGDVDVPYALQWARRPL
                     TFTIQNLVLWGLGAPLGVLSLGGLVWMTWRIIRGDWKKHILIWSWTLFVLVTQSMNFV
                     KSMRYIISIYPALALIAAWTIFKLLEKGSAVGRKRRLGHFNWGRTLSWLTLVVTLLAT
                     TLWAAAFSSIYTKPVTRVAASEWIYENIPGAINLKISGLDGQIFTQPYAYQNTAVISS
                     ENPYRYQITAVKNGQVNSVSVQHVLLHSGTTLQNTLLLSIYEYGQNGKHLAGAGFTQA
                     DFSGLSDNSGDQALIQLQTPVPLEAGRHYELEFSVAEPGTFLRIGGSVAINTSQWRIY
                     LPQPVFQLTSASPYQLLIHPRETGKLDEIVLNRVADQSEENQVTRVKAFIYAPDDPQT
                     ILASAELVSRFANDPDLRGQPYTLKFDEAPLLEKNKGYSLLLQVEGSAVLGVYNSITA
                     LESTWDDALPVGLYGYNQFGYIDGIYGNHLNMELYWDDNAEKLERLTSILDQADALFI
                     SSNRQWGTIPRVPERYPLTSQFYRSLIGCPEQKDILWCYAVAKPGIFQGELGYKLTAV
                     FQNDPALGPFKINDQLAEEAFTVYDHPKVLVFEKTSAYDPDKTAKTLKSVDLTSVVHK
                     TPRQASRISGNLMLTAKEEDIQTAGGTWRELFPPESPLNRQPWLAAVTWYVVVMLLGW
                     TVYPLVRFFFRGLLDRGYGFSRLVGLLFLAYLTWMAGSMGLPSSRVTISLVFLLLLST
                     GVAAAALRRGEIWQEIRKNSRRFLIIEGVFLALFVLDLAIRIGNPDLWHPWKGGEKPM
                     DLSYFTAVLKSTTFPPYDPWYSGGYINYYYYGFVIVAVLVKWLGIEPAIAYNLILPTL
                     FAFTGIGAYSVGWNLFARLKPDDRSSPTGEKRSYQAGLAAAAAVLIAGNLGTLKMLWQ
                     GLQRLAAPGGIIDGAGFFERLGWFFSGVMRFVNGARLPYGFGDWYWNPSRAIPGDVIT
                     EFPFFTFTYADLHAHMIALPITLLVLGWVVSILFRNWEAHSSARFQTAAGLIITILFG
                     GFTIVTLRPTNTWDLPTYFLLAVFVLFYSSYRNAQPPSGFLPAAPCWLRRILYSMIFI
                     GVLAAGVMLFYLPFTEKFGQAYGMIDVWKGDHSPLGSYLVHWGWQLFLIVSWFAWETR
                     DWLASTPASAIRRMKPYLGYLQVIGVLFTMVVVLLTLLRVHIGWLVCILGVWALVLLL
                     RPHQADSKRLVLFMTGTALVLTLFVELFALQGDIGRMNTVFKFYFQAWTLLAIAASAA
                     LLWLIPAATSVWKSGISTAWQVLTVILIAGSLLYPLTASMDKIRDRMSASAPIGLDGD
                     AFMQSATYFDQGVEMDLNQDYQAVQWMRENVQGSPVIIETNTVEYRWGNRFTIYTGLP
                     GVLGWNWHQRQQRGFLDYNGINNRLNDIPLFYQTTDIEQALEILKRYQVKYIILGQME
                     RAYYSGAGLDKFANYDGLYWKEVFHEKDTVIYEMID"
     gene            33359..35932
                     /locus_tag="PLW25_00150"
     CDS             33359..35932
                     /locus_tag="PLW25_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOS52928.1"
                     /translation="MSQAINIFVWYLVGTFVGVIALPLVFRFFPSLPSRGFALARPLG
                     LLIWGFTFWLLASFGVLQNDLGGQVLAVLIMLAFSLAELKGTRKDALLTWLRQNLRTV
                     LTIEGLFLFVFLAWVLVRGMNPEVAYTEKPMELAFINSILRSTSFPPQDPWLSGYAIS
                     YYYFGYVMVAMLTRFSGVAAATAFNLANASWLAMTAVALYGIVFDLSLAGAWNSHHQT
                     AKQVEKFARRAGFLGPLFVLLLSTYEGVLEILHAGGLFWKVDPQGQLSSRFWNWLSIL
                     DLNQPPTQPFDWIPSRGGGWLWWRGSRVLQDLNLGGQHIEVIDEFPFFSYLLADLHPH
                     VLAMPFTLLAIALAMNLFFDGGTYLQQSRRLLDWARNENFWLIALVLGSLGFINTWDF
                     PIYVGLFCLIWSFKSYRATGWKPGLLAEFLKNGLALGVAGMVLYLPFYLGFGSQAGGI
                     LPSLEFMTRGIHFWVLFAVLLVPIIAFCLNQAKRKLGWSDFWKGARFTLALLSVLFIL
                     MVFYGALLLSFQAWGAGLSASSNLFLSALGVKMTQGGAAFAGVHAGYPAGEVLKASLL
                     RRLQAPGTWLTLAALLTITLSVLLKRKTAQSVEIPAEGEHLRITNGAEELIFFLILFG
                     LLLTIIPEFFYLRDQFGTRMNTIFKFYFQAWVFWGIAAASGSAVLFRQLRGWRGSVFQ
                     IVWILVVAGGLAYPAIMLADKTNSFSMKGWTLDGNAYLQKFNPEEYAAIGWLQDAPMG
                     VVVEAVGGSYTDYARISTRTGLPTLLGWPGHESQWRGGGEEMGSRFADIQQLYETTDW
                     NEAWRTLRDYNVRYVYIGNLERSTYRVSPSKFDSHLPLVFSNSSTRIYEVTAPSGEAQ
                     P"
     gene            35929..37614
                     /locus_tag="PLW25_00155"
     CDS             35929..37614
                     /locus_tag="PLW25_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52929.1"
                     /translation="MNGNLDSRKAVPLELLVYLVVLVIAAALRFSNLGGLPLTDHEAS
                     LALQALRLSKGEQVLISGEPGYIALTTLWFSLFELSDFTARFWPALAGTLLALTPLLY
                     RERVGKAAALVLAGLVAVDPIMIGASRSAEGSMLALLGLLAAVGFAMRSKPLWCGFAL
                     GFALAGGPALWPGVICLAVFLLINCKSLRQVDNRFLALAAMTAMVTLVLLSTLFLINP
                     PGISAFGSSLVDYWGSWRSENPAPLKGIAAAWGSSTLLVLVLSIWSLIQGFVRGEARA
                     KWLGIWAGAALALILMNPARQLFDFSWATVPLLALAAMKLVELFPSFSSDNRIVFIAE
                     TVLVVALVVFSFMNAVNLVNNPLQSPEEYRNRAIGAVLPLFLLLGMTVLLAWGWSGAS
                     TREGFIAGVLLLAGFFTLANSIKAASLGSRPEIEIRRDLAAVVGSEELLRTIQDISRA
                     RTGIENRIDVLIVDLQSPSLEWALRSFEKAETAAVFDPTASPSLVITDVNQQITAGAS
                     YRGQKIIWQEEPDMKNMTIIDWLKWSFFRIAPLENREIILWARNDLFPGGNIP"
     gene            37611..>37834
                     /locus_tag="PLW25_00160"
     CDS             37611..>37834
                     /locus_tag="PLW25_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOS52930.1"
                     /translation="MSTRGAAMRKQQKPLVIAIDGPAASGKSTLAEKLAQRLGFFYFD
                     TGVMYRAATLAAMKDLGGVEDEKAVTRLTAA"
     assembly_gap    37835..37934
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <37935..38156
                     /locus_tag="PLW25_00165"
     CDS             <37935..38156
                     /locus_tag="PLW25_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOS52931.1"
                     /translation="SPEERARRRLKEMQLRGETHSFEEVLQSIIRRDGIDSTRQHAPL
                     KPAVDAVIIDTDGKSIEEVLEETLAIVQP"
     gene            38202..39533
                     /locus_tag="PLW25_00170"
     CDS             38202..39533
                     /locus_tag="PLW25_00170"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOS52932.1"
                     /translation="MCDTIVALGNSTVDGNVIFGKNSDRHPNEAHEVVIFPAAQHPTG
                     TTVKCTYIEIPQVPATHQVLLAKPFWIWGAEMGSNEHGVTIGNEAVFTRTPYGREPGL
                     IGMDLLRLALERTDSAEAAMMLIIHLLEEYGQGGNCGFGHKMYYHNSFLICDPKEAWV
                     LETSGKEWAAEKVRDVRSISNAITIGEKWDLCSANLVSSAIKSGWCRSAAEFHFARCY
                     SEPIYTTFAAGRSRQACTADSLRAQKGNITATSMMRVLRAHTREIKPTWSPDRGITGA
                     DVCMHAGWGPIRENQTAGSMVSVLRSGGRSEHWVTATAVPCTSLFKPVWLDAGVPSTG
                     NAPTGEYDHAALYWRHEALQREIARDYQARIAVIKDAQATFENELMAEVARADNATAQ
                     QRRLISENAFRRADEIEEDWYAQIKGMKITRQNTLLYRSAWNKFNAQAKMK"
     gene            39553..42828
                     /locus_tag="PLW25_00175"
     CDS             39553..42828
                     /locus_tag="PLW25_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS52933.1"
                     /translation="MIRIHLFNAGPFFDTPYQLVGWIGWFVMAALILWVARKDLTRQR
                     TSLIWKYFFALGIMAIVSTLFFGFELPPYSKVPLPNIPGEIQPPAVMVLMMVPIMLAA
                     GILGTWPAAVLGFIAGIFSALWNTHSVFTPLEISVLAVVISLALRQNYRTRFFAFLRR
                     PIGAALLAAFFSIPLFLVSTFFGTNGNLAAKLDYAFTRSWVLIGVSTIQLLIAALICE
                     VFRAPNSRYWVKFGSFEPSPVETGLQSRILYTALPLVWILLTTLVIADWFVAGRAARD
                     ILVKQLQDTSKIAVENIPPILETGQSLTAALATQKLPLGDTQQLRDLLQSKVREFPFF
                     SQIYIFNLTGSPLTGYPDDEVGEFRLSSEEELGIQLALNGVQMQNYVIHPYPGAESAV
                     ISYIAAIPDEYGLAQGVVITRTDLTMNLFSQPALIALQNLVEEGGQGVIIDETNRVLY
                     HTNPKLIMTTYTGQIPAVSGYFEDPGLTGTRYMNYASVDDLNGWKVILSVPANHSQQL
                     ALRIAIPLLVISLIISVLAYLLLRYLIKTLTQSLVLLANKADEISKGELDNRVEVKGV
                     DEIGRLSSAFEQMRLSLKLRLEELDALLKVSQGIASDLSLESTSAHLLSALRSYGADA
                     ASLVLTRGTKEGWDEGLEAYRSGEQAEEYAYLDHLLLEHLQEESLLIIPSRARIRRMA
                     LPKTAPIPSALAAIALNKSGENPGFIWVVYTQSHRFLEPEIRFLDTLAGQALLAVSNS
                     LLYQKAEVGKKRLESVLSSTPEPVLVAGEDGRLMMVNQAAEEMTLLIQLNAQGTESRG
                     EIISRLLKEFITGTNPTESRAEEMELEDHHIYLVSVSPVAVESKHLGKVCVLRDVTDY
                     KQLEKMKSEYVSTVSHDLKAPLVLIRGYASMLPMVGDLNEQQRDYSQKILDGIDDISR
                     MADNLLDMRRIDSGIQLQVEKVSPSDLLDEVIEEMQPQIKHRKIQILRELTLAQNLTI
                     EADRVLLQRALHNLLDNAVKFSSLGSQVNLRLQVNENSVVFEIQDHGSGIAPLDQPHV
                     FESIRRNTDGSPKKEWGVGLSIVRSIAERHQGRVWLESQLGKGSTFYLEIPLQHQAAE
                     TNK"
     gene            42974..47218
                     /gene="rpoC"
                     /locus_tag="PLW25_00180"
     CDS             42974..47218
                     /gene="rpoC"
                     /locus_tag="PLW25_00180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOS52934.1"
                     /translation="METKSLVALRINLASPETIRSWSYGEVLKPETINYRRLRPEKDG
                     LFCEAIFGPSRDYQCYCGKYKNPRYKGIVCDKCGVEVTRSSVRRERMGHIDLATPVAH
                     IWYTRRIPSYLGLLLDISRRNLDRVLYFAQYIVTFVDDDARQKALRRLMDEINDSELE
                     QANRINAQILEVKLGRDKKVAELQSRLKEIESKAEEQITSKLEPVIQEGQSLERQLTE
                     LSGKAAKKKIFFETADVMIAEKGEIIAPSHIASVQKAVKERLEEIEDEYKKQLDMDRE
                     QLKMAIEQVKAEAEVSMDELRNQLEDQSTESHDLSGRMRDELEELRPFTFLTESRYRE
                     LKSRWGQVFRADMGAEAFYDILRRIDLDKLSEDLWTEVRTTKSKQKRKKATTRLKVIE
                     SFRRSGNRPEWMILTVLPVIPPDLRPMVPLDGGRFATSDMNDLYRRVINRNNRLKRLL
                     ELGAPDVIVRNEKRMLQEAVDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVVGPKLKLYQCGLPKTMALELFRPFVVARLVQHSYAANVKSARR
                     LIERNRPEVYEVLEEVIKERPVMLNRAPTLHRLGIQAFEPILIEGSAIQLHPLVTTAF
                     NADFDGDQMAVHVPLSDKAVQEARNLMLSSKNLLKPADGEPIISPAKDMVLGIYYLTV
                     QDNRKHKGDGRVFADMDEVELAYNLGQVDLHANIKIRMNTWFNAENKRMPKAETRMID
                     TTVGRVLFNRILPEEVQFINFVLEKSGVKDLIADVYDLCGESVTTEVADAIKDIGFEY
                     AMKSGTTIAVADITIPEAKYEIIGSAQQEIDRINRDYRRGLLKEEERDKLVIDIWKKT
                     TEDVGAAVRAGMDPNGNLSVMASSGATKGGFGPISQLAGMRGLMADPAGRIIRLPIQS
                     NFREGLTALEYFISTHGARKGLADTALRTADAGYLTRRLVDIAQDIIINEEDCGTESG
                     IWIRGADDVAGQKLKERLYGRTVAGRIVNPKTGEIIAERGDILDHDLIRKVTDAGVED
                     VYVRSPLTCELIHGICAKCYGMDLGRGSLVTLGSAVGTVAAQSIGEPGTQLTLRTFHL
                     GGVASAGDITTGLPRVEELFEARRAPKGEAVITAISGIAKVLQADRVGDQKLVRVEHS
                     ELVSDEYEIPTDWEILANDASEIVTGDPVARMGDALINAQHDGRVRVEGRKVIVSYEQ
                     KESEDYEIPGTSRMIIRDGDQVEAGQALTEGSINPHTILKIKGREASQMYLLNEIQKV
                     YRAQGQNINDKHFEVIIRKMIGKVQVIRPGDTNYLPMDVVDRLEIKRVNEEMIAQGKQ
                     PARYAEILLGVTKASLSTDSFLSAASFQHTIKVLAGAAIASSEDPLFGLKENVIIGKL
                     IPAGTGFVYGRFIPAVEGETAEVEVAAEPETETKIE"
     gene            47365..>48956
                     /locus_tag="PLW25_00185"
     CDS             47365..>48956
                     /locus_tag="PLW25_00185"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HOS52935.1"
                     /translation="MDMGSVRKIDIDHEMQQSYLDYAMSVIVSRALPDARDGLKPVQR
                     RLLYAMYDMGVRADSAYKKSARIVGEVLGKYHPHGDQAVYEAMARLAQDFSMRCLLVD
                     GQGNFGSVDGDPPAAMRYTEARLTSIAMELLSQIDRDTVDFVRNFDDSLDEPLVLPSA
                     IPNMLVNGASGIAVGMATSIPPHNLVEVIDGLAFLLDNWQKMEDITVTDLMKFIKGPD
                     FPTGGIILQEHEQDEILAAYATGKGRITLRGRLNLEEMSRGKSRIIISELPYMTNKSA
                     LIERIAELTREGLLEGISDLRDESDRQGMRIVIELNKAADAEAVLRELYKRTPLQVTF
                     SINLLALVNNEPRLLPLKQALRVYLEHRIEVVKRRSEFDLRKAQERIHILEGLRIALQ
                     NLDEVIAIIKKSTDTDDARERLIKKYKLTLIQANAILDMPLKRLAQLERKKIEQEYRE
                     LVQLIKELLALLRSPAKQRQVVGDELKEIRTKYADRRKTQIVNLKKGKAAADLLTVKD
                     VTPVESVWVVMDEQGRIGKINA"
     assembly_gap    48957..49056
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <49057..49515
                     /locus_tag="PLW25_00190"
     CDS             <49057..49515
                     /locus_tag="PLW25_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOS52936.1"
                     /translation="AHYLVVTRSGMAIRFEGSEVRPMGLLAAGVNAIKLSAGDEVAGM
                     IEIARKGDVVLVASNGAGWRVEPDGFPLQGRYGQGVIGCRMADGVGLIGVVFGKKNFQ
                     YTLHLKKAAAKTCRVDDIGYTKRAGKSEQILQLASGDRVERIVRMMDSAE"
     gene            complement(49532..50638)
                     /locus_tag="PLW25_00195"
     CDS             complement(49532..50638)
                     /locus_tag="PLW25_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOS52937.1"
                     /translation="MTLTGKGMMIWKIPSCEAGNPSAIARVAASSGFSHVLIKIANDT
                     RPYNVDSSGRDLIPPVVDALRAKGIQVWGWHYVYGYDPVGEANIAVSQTRKYALDGYV
                     IDAEVEYKQAGREAVAKIFMTQLRKGIPSTPVALSSFRWPTYHRSFPYSAFLEKCDLN
                     MPQVYWMQAHNPDYDLKRSVNEFKAISPFRPIVPTGPAYGESGWYPSPSEVKLFLDTA
                     RSLGLTAANFYSWDYSRVHLVPVWDEIARYSWPAQGSVSPPSSDPVTRLFDALNAMQY
                     DPMLEIYAPNAVLVTPQQTIQGNANIRGFFGQMLTNQLNDGDFRVTSKEVKDNVIHYT
                     WTCHSRRGIVNNGKDTVGLKDGRVLYHYCYYSIE"
     gene            complement(50654..51238)
                     /locus_tag="PLW25_00200"
     CDS             complement(50654..51238)
                     /locus_tag="PLW25_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOS52938.1"
                     /translation="MNREEALKLVRQYVKNENLVHHMLAVEAAMCFYAEKLGQPVETW
                     SVTGLLHDFDWEIHPSAEQHPLEGAPILRQHGVAEEVIQAILAHGQNLNIPRTSLLAK
                     GLYACDEITGLITAVALVRPSRSLYDLEASSVKKKWKDKAFAAGANREEIARGAEEFG
                     VPLWEHCENVIIAMRAIATQIGLAGNLEPGSSPG"
     gene            complement(51243..51890)
                     /locus_tag="PLW25_00205"
     CDS             complement(51243..51890)
                     /locus_tag="PLW25_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOS52939.1"
                     /translation="MLLKLVLFSILLLLVLFGLPKVVTSLSAAGRIYSVADAPSAPVA
                     VVFGAGLQKDGTPSPVLKDRVTAAVQLYQAGKVKKLLMSGDNRFVDYNEPASMRDYAL
                     SQGVPEEAIVLDFAGRRTYDTCYRARDIFGLDDILVVTQKFHLPRAIFTCSGMGMKVN
                     GVIADLRDYHDHAERFWRIREVPATLVALWQVWVSKPLPVLGKPEPIFAEQSDNH"
     gene            complement(51975..52796)
                     /locus_tag="PLW25_00210"
     CDS             complement(51975..52796)
                     /locus_tag="PLW25_00210"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOS52940.1"
                     /translation="MLREWRLILSPPASGAWNMGLDEAILFSTAQKNSPPTLRMYAWQ
                     PATLSLGFAQPHLDVDLAALEEEQWGLVRRPTGGRAILHVDELTYAVAGSDDEPFLAG
                     GLLESYCKISRALLTGLARMNVTAFGDKAYTNEPPDNKKNPVCFETPSNYEITVDGKK
                     LIGSAQSRKYGGILQHGSIPIKGDLARITRVLQFPSEQARQTAANRLIARASTLEAVM
                     GTAPSMETVAEAVITGFSKAGNIHFSSSQPSEQELKMAAELVSAKYGSAQWTFRI"
     gene            53137..54369
                     /locus_tag="PLW25_00215"
     CDS             53137..54369
                     /locus_tag="PLW25_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOS52941.1"
                     /translation="MTKKLFPLFSVLLVAVMLLAACGPKEAGPVTINLWTKEGEADGG
                     LQYVQALADAYTAAHPNVTFVVTNKEVETLREDFQTASLANAAPELLWTVSDHLGPFT
                     TADLIQPVDKLVDLGKYVGSATDAVKTPDGKTWGVPIANGNHLMLLFNKSLMPVAPAN
                     TDELLKMGADFVAANPGKYALAFNETEAFWFVPWLGGFKGKVFAADGVTPTLNTPEMI
                     ETFKFLAELKAANIMPAESNYDVMDAAFKEGTAAMIINGDWSLGGYKEALGENLGVAP
                     IPKVVATDTYPAPYTSGVFFMVPKGLEGEKLDAVVDFMKFATNKENQVEMVKKLTRLP
                     ALTAALSDNVITEDPILAGSAAQMSYGTPMPTNVEMRCVWDAIKPELLPTYAGTTSPE
                     TAAANAQKAAETCIAGLE"
     gene            54481..55488
                     /locus_tag="PLW25_00220"
     CDS             54481..55488
                     /locus_tag="PLW25_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOS52942.1"
                     /translation="MITSEVILKSLGEVGTTVLLILIGTVLVEALLYLILMKWLKYKY
                     ALPFMLLAPAAIGLIALVIYPLIWELRLSFTNMSLKAFRDPAFIGIKNYVRVFTEPVL
                     KQKMFFPLLGQTILWTVINITFHVGGGLGLAMLLNRKMRGKGIYRAIIILPWAVPQVI
                     AVLAWRGEFNFAYGYFNIILTKLGLPGVQWLADPVANFAAMCMTNIWLGIPFMMVTLL
                     GGLQSISSEFYEAAEMDGANAWQQFRNITLPLIQPVLTPAILLGTFWTFNNFNVPFFI
                     NQNELESSDILVTGLFRAAFQYNRYGFSAAFAFVIFALLLLFTIFYMRYTRSLKGAYE
                     S"
     gene            55485..56366
                     /locus_tag="PLW25_00225"
     CDS             55485..56366
                     /locus_tag="PLW25_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOS52943.1"
                     /translation="MSAKHTTPKKSFFRSGREDSPFKRILIHLVLIISCVIAIFPILR
                     ILAVSLRPGNRLMSTDLAIIPPDATLESYRIILFEKPFLSWVWNSLAITISTALIGVI
                     LAATAAYAFSRWKFPGRSVGLVFLLATQMIPASMLMIPIYILAIKLGMVGTYRGLVIA
                     YCVTSIPFSVWILKGYYDTIPVDLEEAARIDGCSQLQAFLRVLLPLSTPALAITFLFN
                     FMAAWNEYLLARVMLGSQESLLTWPLGLQRLQGQFQTQWGQFSAGSILVMVPVMVLFL
                     YSSKYLISGLTLGGVKG"
     gene            56449..59502
                     /locus_tag="PLW25_00230"
     CDS             56449..59502
                     /locus_tag="PLW25_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HOS52944.1"
                     /translation="MKKTFKYLIFILVLGLILSSCTSPTGDTRKGIGRKDVLYLNLTW
                     HQHQPLYYKDDQGVYTRPWVRVHATKDYYDMAAMIKPYPNVRVTINLTPVLMKQLDDF
                     TQNGAKDYYWVLAEKPAAELTDADKTFLLSRFFDANWDNIIYRFPRYKELLNKRGGTS
                     AEAIASAVSQFSEQDFRDLQVWFNLAWIDPEFLAQSPLKELVEKERGFSEADKAVLFN
                     EVRKIMEMVIPVHKELQDSGQIQVITTPYAHPILPLIYNSNIASVGNPAAELPARFSW
                     PNDAIVQLQKSVDIYQQHFGRAPIGLWPGEGAVSEDIIPLVADAGYQWMQTGEVVLAQ
                     SLGLGQFTRDSAETVQEADELYRPYYVTTKDGRRVAIFFRDLVLSDKLGFTYSQTPGE
                     QAAADLMQRLENIRLQLKKQKAQGPHIVSIVLDGENAWEYYPNDGKDFLNAMYRMLNE
                     SETIKMVTPTEYLELFPKQRELDTLFPGAWFSPNYDTWIGEAEETQAWNYLGKTREFL
                     AKYDVGKTKQASPEAIATAQDYMYLAEGSDWFWWYGADQDSGVDEYFDTGFRALLAKV
                     YESLGEPVPAFVRVPIIPRRPEQPVQAFSGMMAVVVDGKTDQKEWATAAAYPANGEDG
                     LEMLYYTMDAQNIYFRLDFDKEREEPESVGIYMSIPSAAANDPFALTEDAPDSLLLGI
                     PASHLFECNAGLVSGYEVFAGKWQLNPELKATAEMGEGLLELAIPLTSLGELSAGDDI
                     RVVIVVQPNSQVLPLSGPAQIVLPDLGLSDLVLEINDPEGDDHGPGTYTYPNDGVFSK
                     QNFDIESFSVATDAKNVIFTFKFYGPVPNPWGSGINLSLQTMDVYIDQDPGKGTGNRM
                     LLPGRNASLTARNGWEYVIWAEGWNYGIYAPDPATGEPKQQNSSYKILVDPARQTVTL
                     RVPKEALGDGNPAEWGYAAVVLSQDGYPSTGVWRVRDVQQTAAQWKLGGAPDDTNHTR
                     IIDLAWPAEGKGTQEEFLSTYISSKAAIETLSEKDMPQIPILLVK"
     gene            59646..61160
                     /locus_tag="PLW25_00235"
     CDS             59646..61160
                     /locus_tag="PLW25_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOS52945.1"
                     /translation="MTIQTPDWLKDAVFYQIFPDRFAKSERVKREGLKLEPWDTPPNP
                     YGFKGGDLYGVIEKLDYLVDLGVNAIYFNPINASASNHRYHTYDYFKIDPLLGGDEAF
                     RDLLHAAHSRGMHIVPDGVFNHASRGFWQFHHVLENGEGSPYVDWFYFDPERLKRHRP
                     WGAYPDEREQQQLNNGVGSFDAIGYTAWWNLPALPKFNTQTQAVREFIFEVAEYWLRF
                     GADGWRLDVPGEINDDSFWQEFRRRVKAVNPDAYIVGELWLESQRWLKGDQFDAVMNY
                     LVSAALMGWLIGDKMPEFAYRIGGLQRVMRPFSAAEFGDRIEYLLNLYDPEINYAQLN
                     LLDSHDTPRFITTASGDESALRLAYLFLFTFIGAPCIYYGDEVGLDGGPDPECRKSFP
                     WDESRWNQGLHAYMKDLIHLRNAHPALRRGSYKRIFSDNEALAFIRQYMGETYLVVIN
                     AGSKERQLHLDLTQTGVSSRAVESLFGLGRATLEGGSLCIEHLPSRQGVVYRLE"
     gene            61379..63070
                     /locus_tag="PLW25_00240"
     CDS             61379..63070
                     /locus_tag="PLW25_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOS52946.1"
                     /translation="MKVNSPRPTIEIVLPDGRVYAGNRGASLCELLQMLPEWDNPPIM
                     GAVVNGELKELTATLEMDARVRLVTMKDDDGARIYRRSITFLLEAAFEELFPKSLMAL
                     DHSVSAGGYYCQVSKGNQLGSEDLSRLELRMRELVEADLPFERQLVPLDEAISYFESK
                     GLKDKVQLLKYRQKEYLVLYRLSEHRDYHHGYMVPSTGYLKWFALQQMGEGFVLRFPR
                     RESPKQLSPMPSYPKLLSTFRQYGNWLQRLGIGSVGELNASIDAGNIREIILVSEALH
                     NLQIIDISQSIADKSEEARIILIAGPSSSGKTTFSKRLSIQLLAQGFSPFPIEMDNYF
                     VDREKTPKDEFGQYNFEALGALDTHLLSDHLKRLIQGEKVRLPKYDFHTGKRQQGEEI
                     QLRHDQMIILEGIHGLNPQLLPEIDPSSTYKIYVSCLTQLNLDRYNRISTTDTRLLRR
                     IVRDARERGYTAQETISRWESVQHGEKEYIFPYQENADKLFNSALVYELSALRLHAEP
                     LLRQVPYGTKEYVEAKRLLAFLEWFLPIDSRMIPDNSLMLEFLGGSILKDFILWK"
     gene            complement(63113..64342)
                     /locus_tag="PLW25_00245"
     CDS             complement(63113..64342)
                     /locus_tag="PLW25_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOS52947.1"
                     /translation="MHTKMQTPWEYRYSQRTQRMKASAIRELLKVTEQPDMISFGGGF
                     PAPDVFPVEEFKKACNYVLEHKGAEVLQYGGTDGYVPLREMILRHAINLGINVDIGNI
                     LITSGSQQALDLLGKIFINRGDRILVESPTYLGALQAWNAYGAEYITVPVDEYGMITE
                     KLEAALRIGPKFIYVLPNFQNPTGTTLSLERRKKLIHLADRYGVPIVEDDPYGQLRYE
                     GESLPAVELLDSQMRVQNGTYTGNVIYLSTFSKILAPGLRLAWVIAPHGVISKLALAK
                     QGCDLHTSTFNQIVAHEVGQHGFIDRHITVIQKVYRERRNAMLDSLEEHMPEGVKWTR
                     PHGGLFLWVTLPEQFDTTEMLPEVIQEKVAYVPGEFFHPDGSGKNTMRLNFSFCSPER
                     INEGIARLGRAFKKKIA"
     gene            complement(64375..67503)
                     /gene="gcvT"
                     /locus_tag="PLW25_00250"
     CDS             complement(64375..67503)
                     /gene="gcvT"
                     /locus_tag="PLW25_00250"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOS52948.1"
                     /translation="MTDYLVRGSLSEIDPAVFNLVNYEVERQFRKLILIPSESTAPQA
                     VRDSLSSVFQNLYAEGYPDEETRLMSEKEILDYPVRLSYYRRYADPRYYKGVEYADVV
                     EALARRRCAEVFATDRVTADQLYVNVQALSGAPANNAVYHALIEPGDTILGMNLLHGG
                     HLTHGSSVNRSGKLYKVAHYNVDPETEQIDYEKLEAQAKEVMPRIIICGYSSYPWIPD
                     WSRFRAIADAVGAYLLADVSHIAGLIAGKAIPSPVGHAHVITFTTHKTLCGPRGACIL
                     TTDSALAKKIDKAVFPGEQGGPHVHVFAALATTFKIAATAEFQQLQKQIIQNCVALTA
                     QLKARGLRIPFNGTDSHLGNIDCKTIVGPDGTTLSGDMAARILDIAGIVLNRNTIPGD
                     KTALNASGIRYGTPWMTQRGLTAENMREIGNLIADLLQAITPYTIEMRKGPSPRAKID
                     FNVLEQTKIKVRNLCASAATDLPHGNSGYPHFYYLDDQPRFKGKLVSLDLKGDRVLYF
                     VNYCFSSDVEALQNGQTQPTSLITPLKKVEGSLTRVEDNLYRFSFQAENFGLAATFLR
                     ALSDGYIAFDNDLPARIPGPVVVEESELVAPMPSSGKEMANNKAWYIGITNSNGNALP
                     SFKWEEKSSNELMRTPLFEDHKRLGAKLIPFAGWEMPVWYTSVVEEHLACRNAAGLFD
                     VAHMGVFQAEGSQAALFLDSVCGNDIGSLEIGDSCYTHFLDPDANVIDDTLVYRRSAD
                     KYLVVVNASNDAKDWAWLNAVRNASVAVDNHRPWVKAFGRGVVLRNLRDPREGADMRV
                     DIALQGPKSRDILLALGCDAATQRKVKALKRTELCEAVIGGYDLIISRTGYTGEKMAF
                     ELFVHPENVSSLWNDLMKVGEPLGLKPCGLGARDSLRTEAGLPLYGHEMGGERNLGVA
                     EAGFGSYVKTYKPWFIGRESFIKKELTRKGVVVRFRFAEKGVRMAHNNDPVLDEKGRV
                     IGHVTSCAIDKDGLLTGQAFIETRYAVEGTVILIYQGAPSQAGKAPSQLNPGERVTLP
                     SRATVESRFARL"
     gene            67879..70296
                     /locus_tag="PLW25_00255"
     CDS             67879..70296
                     /locus_tag="PLW25_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOS52949.1"
                     /translation="MITPENTINEHPELDYSLKKARKMGVILLIFFTTLWVLSLTGLN
                     LFLRWSIEQSMFETETGIQDVRWLVHLVCGVFLFIPLLALSFAVKVPRVKIMLRLWTV
                     GAAFVLLSVPPKVLYLTAQVQSNLLLCGVLLLMTLVLFLLRNKQTSGSKTLPPSGLAG
                     PVFLACAALATPWVMWGALGSVEDSLSALLLGAAFALFFINAVVVDYFDKTQPPGSLV
                     NRKDFLFDGFVLFIFLFVVVSGLSQNGSQIVLCLSIPACGWLLAALSTAGRNRKNHAR
                     LATGLVSGLMLALPLAFFDMDELALFISGTDGEVFFWANRAGWNTLTFQIAFALVVLI
                     FFKQLERVHLGRRINAMLLVLSLAEVLVLYFTAGQPGFFGDRLFVVMKEQVNPTQFNE
                     INPLLEKKLALYQALTETAVTTQEEVTTQLAKWHIDFTRYYLVNGLEVNTGVLMKSML
                     EKMDGVDRVLDSPRLRPLPQPPPRMEGEITIPPEDSLWNLQMINLPLVHEQLGVFGEG
                     IIIGQTDSGVDGSHPELAGSYRGAKGSNDTNWLDPWNHTVAPNDLSGHGTGTTAIIVG
                     ATRGIAPKAQWIACTNLARNLGNPALYLDCMQFMFAPYPQGGDPLLHGKPELGAMVVN
                     NSWGCPRIEGCDADVFKPAVEILEAAGIFMSVSAGNNGYFGCSTISDPPALYEHVFSV
                     GSVDSAGELSAFSSKGPVTSDGSGRSKPDLLAPGEQVLVAAPGGTYTYSSGTSFAAPH
                     VTGVVALMWSANPKLIGEVALTRQILQESAQPYTDGSTSCGTPQNESGAGILDAYAAV
                     KAAIAVK"
     gene            70339..70935
                     /locus_tag="PLW25_00260"
     CDS             70339..70935
                     /locus_tag="PLW25_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS52950.1"
                     /translation="MKKQSAAPTESLTDQNAVQLACNGNAEAFSYLYEQNVKRIYNYI
                     FYRIGAEADAEDLTARVFHRAFAHINSYVEKGVPFTAWLYRIAHNLTANWYRDNHRRR
                     EVSLDDHFDLPHLSDLPERTIEMNQEKELLLKAIRRLPADRQQLILLKFLEDLSNGQI
                     AIIMGRTEGAIKSLYHRSLISLREELKRISGVETSDFR"
     gene            70948..71190
                     /locus_tag="PLW25_00265"
     CDS             70948..71190
                     /locus_tag="PLW25_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52951.1"
                     /translation="MEHGLEQNGRPGLWGLEQKLSNLLKPVTPDPEFVDSLKMKLTRA
                     PAVLVETSRRKMGLLVVSFGLFAGAFLVWLLRKRWQ"
     gene            complement(71198..71425)
                     /locus_tag="PLW25_00270"
     CDS             complement(71198..71425)
                     /locus_tag="PLW25_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOS52952.1"
                     /translation="MMYGTTWCGSTKRARRLFEDEKIDYEWIDIDQDETAAKFVESVA
                     NGFRSVPTIVFPDGAILVEPSTYQLKEKLGL"
     gene            complement(71450..71785)
                     /locus_tag="PLW25_00275"
     CDS             complement(71450..71785)
                     /locus_tag="PLW25_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52953.1"
                     /translation="MKISERIQKEMDMAFAARKQGNEGMARVCARRAAGIVVRDFLDQ
                     HGIEAAGLNNFEILVDESVRKSLPASVLPALQHLTMRVDENYALPTGIDLLADAQAVI
                     QTLLKEVSE"
     gene            71864..72778
                     /gene="fmt"
                     /locus_tag="PLW25_00280"
     CDS             71864..72778
                     /gene="fmt"
                     /locus_tag="PLW25_00280"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOS52954.1"
                     /translation="MSEKTVFMGSPEFAASILAALGNHYDVRGVVTQPDKPAGRGKKL
                     TPPPVKTLAERLGYPVIQPEKMKDPGVFEQLHAWNPDFIVVAAFGKILRQNVLNLPRL
                     GCINVHASYLPRWRGAAPVQAAIIHGDTTTGVSIMKMDIGVDTGPILIRERVKIEPAD
                     DAASLTSRLATVGATLLLQALDGYINGNLSPVPQNENGATYAPMINKEDGLLDFSQAA
                     EMLERKIRAYSEWPGAYIRLGEEALKVRRAKVVPGSGVPGQRETCNGYPCIHTSDGKL
                     VLLEVKPAGKNWMNAADYLRGSRSWISN"
     gene            complement(72927..75503)
                     /gene="mutS"
                     /locus_tag="PLW25_00285"
     CDS             complement(72927..75503)
                     /gene="mutS"
                     /locus_tag="PLW25_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOS52955.1"
                     /translation="MAEDVTPIRQQYLDIKKQYPEAIVFFRLGDFYETFDHDAQITSR
                     ELDIVLTSRNVAKGQRVPMAGIPYHAAENYLARLIDKGYHVAICEQVGEQPAKGIFPR
                     EVVRVVTPGTVIEPGLLHSNRNNYLAAARVEDGQLSVAYADITTGEFAVTTIESRDPL
                     SDLRAELTRLDPAEILVPESLKLNTAFSGHITSLPDWRFETSRCREVLLRVMQVTTLE
                     GYGLENAPSAIQTSGAILQYLEQTQPAALSMVKSISTYSTGEFMTLDAATRRNLELTE
                     TIRRGETQGSLLSILDHTLTPMGHRCMRQWVSKPLLRIDSINERLDIVAVFHQHSLVR
                     ADFRVQLQHLGDLERLANRLTGNVALPRDLAAIRAILQKLPAIRDTLSQDVLQLSHKL
                     IDFDLCPEVLNLLEQSLADEPPATLQHTGVIRVGFSEELDQVVEMSNHAREWISNLEA
                     VERERTGIKSLKVGYNKVFGYYLEITHSNTAQVPAEYIRKQTLVNAERYITPEMKEYE
                     ALVLNAEERIREIENRLFKEVCQRLLPHLSALLKSARLLGELDVLSSLAEVTAIHAYV
                     RPVLAEEPMLNIVDGRHPVVETTLQSERYVPNDAVMETGEIIRIITGPNMSGKSTFLR
                     QVALIVLMAQIGSYVPAASATIGIVDRIFTRIGAQDEIHTGQSTFMVEMIETANILNH
                     ASSRSLLILDEIGRGTSTYDGLSIAWSVIEYIHNHPRLRARTLFATHYHELTQLSDLL
                     PGIRNYNVAVAENGTQVIFLHKIVPGGTDRSYGIHVAQLAGIPRPVISRASEILQQLE
                     ASSGSTIKVDLPSSQQLTLFPETNPLLDELKAIDLNTLPPIEALNILYEWRRRFLSAE
                     ES"
     gene            complement(75574..76218)
                     /locus_tag="PLW25_00290"
     CDS             complement(75574..76218)
                     /locus_tag="PLW25_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOS52956.1"
                     /translation="MVEHLEFVKNYTDLSTDRGFQFEFTCARCESGYRTTFKPSLTGK
                     LSGALDTASSLFGGFLSQAANISDRVHSAAWERAHDENFTEAVKEIRPYFIQCPRCNA
                     WVCREKCWNKKKGLCKDCAPDLGVEMAAAQASKSVEEVWAHAAMAEEDKKLGTEYWRE
                     GITATCPNCEKPLAANAKFCPECGYALKPKDKCPHCGSKILPDAKFCGECGQKL"
     gene            76325..77260
                     /locus_tag="PLW25_00295"
     CDS             76325..77260
                     /locus_tag="PLW25_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS52957.1"
                     /translation="MQRVQTCDIFTRIQLGIGTWAWGDKLVWGYGNSYSEADIREAFK
                     VCVGGGIRFFDSAEVYGQGKAETILGELCAQEDEEFVIATKMMPFPWRLFHNSLKFAL
                     QNSLRRLRVNQVQLYQMHWPLPPVKVDTWMDRMAEVVDAGMVKAVGVSNYNLEQTITA
                     HEALKKRGLRLASNQVEYHLLERRIEQNGLMEYCLEEGIKIIAYSPLAMGILTGKYTP
                     ENPPAGTRAAQYNRSYLEKIQPLIKTLTRIGNDADGKSAGQVAINWCIQKGTLPIPGV
                     KNAAQAVQNLGAIGWQLDAEEMALLDEVSLAVTLP"
     gene            complement(77273..78061)
                     /locus_tag="PLW25_00300"
     CDS             complement(77273..78061)
                     /locus_tag="PLW25_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOS52958.1"
                     /translation="MDFQIAGHQTEGYKINTEVYSGPLDLLLQLIEKAELDITRLSLA
                     QVTDQYLEYMHHLETQDPAEVSAFLVIAAKLVQIKSAALLPRQTIPSLQVEEDPGEAL
                     ARQLLVYKKFKDLSRFLESREEAGYHTYLRLDANPAVQIPSKLDISELTLEALVEAAR
                     EVFSNPNNLPPLSEVVASPRFTIRQKIHVILEALQKMGATTFQQLITTRSSRVEVVVT
                     FLALLELVKRHMVGADQNQLFGEISLQPEGEFSTLDDQELEFVE"
     gene            78128..79237
                     /locus_tag="PLW25_00305"
     CDS             78128..79237
                     /locus_tag="PLW25_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOS52959.1"
                     /translation="MMKSGTINCTYCGEPYPSAGLPFRCTCGGIYDFSRFPDFTIPKA
                     QAVPRGVWHFKDSLGLPAEAPIVSLGEGNTPLMSWSLHHRQVYLKMECQNPTGSYKDR
                     GTAVLTSFLLARGVKEVVEDSSGNAGASLAAYCARAGIKAAIFIPESASGPKRWQIER
                     YGAEVHRVPGPRDNAAEAVLRAAGAGKVYASHAYMPFGLTGIATIAYEIWQSLGREPG
                     SLISPVGHGGLLYGVMRGFDALFSAGLIGKKPIYIGVQAENCAPLFQAYRQQKLNPVK
                     VEISASLAEGTHVSCPVRGAQILERMQNHQGRMVMGSESQLVALYRLSARSGYFCEPT
                     SCLSLVPLLDDKIDLVEPVVAIMTGSGYKTNIILQ"
     gene            79313..80041
                     /gene="rnc"
                     /locus_tag="PLW25_00310"
     CDS             79313..80041
                     /gene="rnc"
                     /locus_tag="PLW25_00310"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOS52960.1"
                     /translation="MHNECNRETPEELAARIFPDLTDILLLSRALTHRSYLNEHPEAL
                     EDNERLEFLGDAVLDFVVGDWLYNRYPEMPEGDLTRMRSALVHTAQLADFARSIQLGR
                     AMRLGKGETQAGGCERTGLLCDTFEAVVGAIYLEMEVEGVKRFIYPMLESCADDILIN
                     HKDEDPKSKLQEWAQGNGYPAPVYTTRDVSGPDHSKFFVVDVIVNGKTCGTGSGTSKQ
                     AATKQAAADALNKLHLSDWKPTQR"
     gene            80038..83637
                     /gene="smc"
                     /locus_tag="PLW25_00315"
     CDS             80038..83637
                     /gene="smc"
                     /locus_tag="PLW25_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOS52961.1"
                     /translation="MKPKLRSLELHGYKTFAGRALFEFPGNITAIVGPNGSGKSNIAD
                     AVRWVLGEQSYTLLRGKKTEDMIFLGSDQRPKAGMASATITFNNEEGWLPIDYSEVAV
                     ARRAYRDGSNEYLLNRQRVRLKDTTELLAQSGLAERTYTIIGQGLVDAALSLRPEERR
                     KFFEEAAGIGLFRSRREEAITRLDATRRNLERVTDILGELEPRLRSLERQAKRVEEYD
                     QVKDDLQVLLKDWYGFHWQNTQKELLRNREVLKNQEIKVEAARTRRGEIEVKVDAARL
                     RLQELRSLLNAWHMETAELHRQREKISRDIAVLEERFRAYQNQDISLKAELSRNEDLK
                     HSNQEKLTALMDEEERINLELAQCAEQLEEIRISFDERKANRDQLETKINLARKQLLE
                     VETRQVQLKAHQRELTGRVESFEDSRKNLLQSIDQNQNGLNKTQKEMEEISAGQVKIQ
                     QSADIYVRDIGIAEEKLAELELQEKEFSGEISRLEAEHARAKAQLEVLHQAELAFSGL
                     SEGARSLLTAASQGKIPAGLRTLSSYLDVPYELETAMSAALGDQLDAVLFGEGVDPER
                     ILSYLENDESGRAMLIPLTAAGKGQARKFAGPGILGVASDLVKPSDLNRGLVNFLLGS
                     VIIVNDRATARRLLKDLPVDGRIVTLKGEVFDGSGVIIAGKENRAGIISRPREKKDLT
                     QQVNRVAEKLAEKKKELEELQKNIISLRNEKSELDKAAREEQAKLQQINATLAGRERE
                     VAKLMQQLEWQNNQINTLVSQAKSAEMDMDGIGKEIAANENAIIELNQTIRQDNEQLR
                     GIPIDEIQQQLVHWNTTQAVTERALREVAGRVNESKQTIAQNQAVIEQVNRRIEEVVT
                     ATAEVRASIEESKKAEGLVNLSLDEFSQKINPAESELRKLESEYSELQHEYSQTQQFE
                     ANADRVYTQAQLEYSRSREALEGLRRRIEEDFGLVTLEYSDEVTGPTPLPLEGVGTLV
                     VLDELPKGLEDSINRQRALLRRMGAINPEARKEYQEVKERHDFMKTQLADLQKADSDL
                     RQIITELDELMHRQFRTTFNEVAAEFKEMFSRLFGGGSARLVMTDTENPTETGIDIEA
                     RLPGRREQGLSLLSGGERSLTAVALIFSLLRVSPTPFCILDEVDAMLDEANVGRFTEL
                     LKELSQETQFIVITHNRNTVQVADVIYGVTMGRDSASQVISLRLDELSDEMVK"
     gene            complement(83689..85002)
                     /locus_tag="PLW25_00320"
     CDS             complement(83689..85002)
                     /locus_tag="PLW25_00320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOS52962.1"
                     /translation="MTKENIERTPKPASKKREARLQRENRQKKIMITVLIAIVAVVTL
                     LVGFGVLQGTVLKNQKIVAKVGDTNIKASQFAKRVQYERLSYVENFTSYAASGYAMFF
                     QSQMLQMQNALDNYIQFGSDVLEKMINEAVVAHKAAEMGISVTDEDIEKELERGFGFF
                     PDGTPTPQPTYAYLPTSTMSAQQLAIITITPTPSPEPTATSEPTATADPSLATPTVED
                     STPTSVPTATQIPPTPTAYTRDGFKQLFNQMLTSINEQFPFTEADFRAYVKSLLLNQK
                     VYEAVTKDIPREQEMVWARHILVASEEEANAVIERLKKGEDFGTIAAELSIDTSSGVN
                     GGDLGWFSRGQMVEPFETAAYALKIGEISQPVQSDFGFHVIQLLGKEVRALSDSQWET
                     LKQKAFTDFIEAAKADIKIEKKDVWASVVPNTPAIPAQYRIAPGQ"
     gene            complement(85084..85368)
                     /gene="rpsR"
                     /locus_tag="PLW25_00325"
     CDS             complement(85084..85368)
                     /gene="rpsR"
                     /locus_tag="PLW25_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOS52963.1"
                     /translation="MSDEITNESERFSGQRRFVARPKICQFCADKNIAIDYKNTDLLR
                     RFVTEDGKIRPRRQTGTCARHQRSLASAVKRARHIALLPFTGERWSESIR"
     gene            complement(85380..85784)
                     /locus_tag="PLW25_00330"
     CDS             complement(85380..85784)
                     /locus_tag="PLW25_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOS52964.1"
                     /translation="MGRDLNKVMIIGHLGRDPEMRFTPSGRPVTTFTVATNRSWNTGD
                     GERHSETEWFYVVSWGNLAEICKQYLTKGQQVYVEGRLQTRRWEDSEGVKHSNVEIVA
                     SEMMILGEKKENAQPNGDLQETDFQAVDEYPF"
     gene            complement(85817..86101)
                     /gene="rpsF"
                     /locus_tag="PLW25_00335"
     CDS             complement(85817..86101)
                     /gene="rpsF"
                     /locus_tag="PLW25_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOS52965.1"
                     /translation="MRTYELVLIFHPELDENALNEALDKVKGWITAGGGSISKVDNWG
                     KKRLAYLIRKQREGQYFLLTAEMDPALTSELDRNLRFHEPVIRYMITVVA"
     gene            86476..86549
                     /locus_tag="PLW25_00340"
     tRNA            86476..86549
                     /locus_tag="PLW25_00340"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:86508..86510,aa:Gln,seq:ctg)
     gene            complement(86673..87851)
                     /locus_tag="PLW25_00345"
     CDS             complement(86673..87851)
                     /locus_tag="PLW25_00345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS52966.1"
                     /translation="MTDPIRKAVFITSVDYESPIQVGDHQLARQFASNGWQVIFISKP
                     ITPWHLVARDNNSFKRRFSTCLSNGRWYTHGKGIICSFVPFAAVIPKNEIFLAKKWVF
                     QYWHKTIFPNIKKNLAVKGFLDVDLLHIRDPLQGYLLDLINAKKKVFRVADNDSGFSS
                     YNPFYTQAEHKIASKVDLVAYSAHQLENRVRQLQPKQAVFLPNGVDFEHFQKVKAIPE
                     LYRSCSRPLIVYAGSIDFWFDFDLINRLAIALPKFTFVIIGPNQNFGSRFIKVKNLIL
                     AGPVPHSELPAYLSYADIGIIPFNQRDFPTLVNAICPVKLYEYMACGLPVVASKWDEL
                     EHIASPALLCSSYEEFLTALMNYQPIKERADTYIQFARQHDWSRRYNALVKLISDVGP
                     "
     gene            88690..89127
                     /locus_tag="PLW25_00350"
     CDS             88690..89127
                     /locus_tag="PLW25_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52967.1"
                     /translation="MKKLAIALILLLATPACSISNQHSVEIQPGLDVSTPAKEVPPPA
                     PQPLPPAQLSGDLVSPDDFEYLGAFRLPGGEDRPQTFAYGGNAMTYNPDGNSLFIMGH
                     DRMPYGDLPDGNQVAEISIPKPVISKNIEDLNTADLSKNFQMC"
     gene            89121..90131
                     /locus_tag="PLW25_00355"
     CDS             89121..90131
                     /locus_tag="PLW25_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52968.1"
                     /translation="MLKRQFSEYDEIPKAGMQYLSRAETGAKIHVAFGEHLQSEQIPT
                     HGWFSPTLSIPDFQGTWFIGNQDLYSVNGYMFEIPAEWADTCTGGRPLGTGRMRDGGQ
                     GGMGPTLFAYRPWNADGSPLPAGTRLDEVTLLLYENAYNTEEIVRSMDGYQHPDEWGG
                     GAWLTTSGGKAAVLFAGTKSNGEKYWYGYIHPDGANLACVDAEVTDFPTCRMADGSVC
                     PQEDYTACCDAVAGECVSNRGWWTTRFDAEFILYDPADLAKVATGQLEPWQPQPYAVI
                     DIDEHLFLSPPEWDLVELGWSDQRRSRIGDVAFDRQNGLLYVLELYGDGTKPVVHLWK
                     VH"
     gene            90816..92216
                     /locus_tag="PLW25_00360"
     CDS             90816..92216
                     /locus_tag="PLW25_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOS52969.1"
                     /translation="MALKQIRSFFRDPEYYRGLLRLMLPIALQNLITSSLNFVSVVMI
                     GQLGEVSIASVGLGNQVWFLLNLMVFGISSGSAMFSAQLWGKKDVHNIRRVVGLTVKL
                     GLVAAAIFFILARFFPGAALRVYSNDPEVIATGSRYLRIVCWTYGFYALSAVFSMASR
                     AVGNVRLPVAVSTSALIINVLIAYPLIFGVRFLGIPALGVEGAAIAGVIARVIECGAL
                     LFFIYRNRENPVAAGPRYLMELDWEFIKRVMVPVLPVIGNEILWSFGITTYNAIYGHI
                     GTKAVAAINIISTVDQVAFVVFLGLGNATAVLVGNLIGQGRMEKAYLYAGRSLILQVL
                     GAVVMGLLVILFAPLILGFYRVDPEVIVNARRLLWILGLGLGVRAANHMIVIGILRSG
                     GDTRFSLVLDGLVIWLVGVPFTAAGAFLFHLPVYLVYALTLTEEITKASLGLKRYLSK
                     KWINDLTGRVSEIIPE"
     gene            complement(92203..93534)
                     /locus_tag="PLW25_00365"
     CDS             complement(92203..93534)
                     /locus_tag="PLW25_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS52970.1"
                     /translation="MIEKSNPGQPGVYLNLPEMSDSQQSKNLKHNFTVNLLDGGLFGF
                     GLGFASFTTMIPLFVATLTSSATLIGLVPAIHNVGWQLPQLLMAKRIARMERVKPYVT
                     FMTVQERLPILGLGIVGLLVPRIGVRVALILTFILLIWQGLGAGLTANAWQIMVSKII
                     PSNILATFFGAQSAAANLLASLGAYLAGTLLVVLNPPYDFSTCFFIASLLLVSSWVFL
                     NLTREEKREMPSGGCVPQPLWHDIRSILKRDRSFTHFLISRFIAHFGMMAFAFYSVYA
                     VKILRMTTVQVGVMTSILMVTMMVMNPLLGRLSDRWSRKWVLVIGSVCAAVSALLAVL
                     IKEVNLFALVFLLNGAANTAFWTIGITMSLEFGEEAEKPTYVGMANTLVAPAAIIAPL
                     IGGLLADAFDYVTAFITSAVMAFIASGILILLVNDPKKPEPYEQPAGFTQG"
     gene            complement(93546..93779)
                     /locus_tag="PLW25_00370"
     CDS             complement(93546..93779)
                     /locus_tag="PLW25_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52971.1"
                     /translation="MPRIKCHYLDCSFLDEGICTAGMVELDPDTGCKTYTVSAEALST
                     EDWDAEEDELEEWEDMEEEGEDDWDELDYDDED"
     gene            complement(93781..94245)
                     /locus_tag="PLW25_00375"
     CDS             complement(93781..94245)
                     /locus_tag="PLW25_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HOS52972.1"
                     /translation="MKSSRPRTSTLIIQISAIIGLLDSIYLAYVKIAKTPIYCTPGLG
                     HCDVVNASRWSILFGIPLGFYGVAGFGLIFLLAAFGLHQRANHPYIDLILFGFSLAGF
                     LFSLYLTSIEIFVLKTVCQWCLLSAMMMSVIFITTIIRLVVKSPSTTASGGK"
     gene            complement(94352..96601)
                     /locus_tag="PLW25_00380"
     CDS             complement(94352..96601)
                     /locus_tag="PLW25_00380"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOS52973.1"
                     /translation="MVSTTKTSPLLENLLDILPDNYSRVDREFIIQAYNFAENAHAEQ
                     KRASGEPYITHCVAVSIILAEMHVQPVVVAAGLLHDTVEDTSVTLQDIQRNFGDEVAK
                     LVDGVTKLTHLPRVSRADQHDAEIFGKNMEKNQEEQEMLLNSQHARDRRRDLANETLR
                     KTFLAMGEDIRVILIKLADRLHNMRTLGFVPEAKRRRIAQETLDIFAPLANRLGIWQL
                     KWELEDLGFRYTNPEKYKEIAQNLAETRLERERQMKAIIDRMAKLINEAGIKVEISGR
                     SKHIYSIYRKMVEKDKPFELVRDLRGVRLIVPDIPSCYAVLGIIHTHWRPVPNEFDDY
                     IAAPKDNFYQSLHTAVLYDDGKPLEVQIRTPEMHENAEYGIAAHWRYKEKGPRNEQYE
                     QRINWLKKLMEWRQEVEDANEFVEGMRTDVFQDRVYVFTPRGDIIDLPAGATPIDFAY
                     TVHTEIGNRCRGAKVNGKLVSLDYTLHTGDQVEILAAKQGGPSRDWLNPTLGLVKTQR
                     ARSKIRAWFKHQNREQNLAQGKEILERELRRLGLQQYDLEKLSRGFDYRTLDDMYVAV
                     GCGDMSLGHIINTLSEGEADESRLFEIKSSTQAKDISENAVSVLGLRNILTTFARCCN
                     PVPGDSIVGYITRGRGATIHRQDCPNILRMTDRERIVRVAWGEPQHTFPVEIEIKAYD
                     RQGLMVDISSVLSNENINLLDIKINVSHNLASIVMKLEIADIVQLSRVLTLIENLPNV
                     MEAHRLNPG"
     gene            complement(96752..97912)
                     /locus_tag="PLW25_00385"
     CDS             complement(96752..97912)
                     /locus_tag="PLW25_00385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOS52974.1"
                     /translation="MNPQNTYDVIIIGAGSLGTPAAFYFASAGLKVLVLETVPSVGQG
                     SNKRAIGGIRATHSDPAKIRLCLRSIEIFSTFKEVYGKDIEWDQCGYSFIAYTPREEK
                     ILKDLLTIQHTYGLVNNWYAKDDLLKIIPDLTPQGLLGGTFSPNDGSASPLLSNHAYF
                     TLAVEKGVTFHFCEPVTGMVVKRGKVISVTTSRETYSAGVVLNTAGASAAEIGRMAGV
                     EIPVRADSHEAAITEPVARFLGPMIVDIRPAPGSSNYYFYQHTTGQVIFCITPSPNIW
                     GEDVNETSSFLPMVARRMISIMPRLKNIRVRRTWRGLYPMTPDGFPIVGWSSQVSNLL
                     LGVGLCGQGFMLGPALGELLTRAVTGDVLPEDADTLRYLSPQREFEGQELLK"
     assembly_gap    97989..98088
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<98089..99814)
                     /locus_tag="PLW25_00390"
     CDS             complement(<98089..99814)
                     /locus_tag="PLW25_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS52975.1"
                     /translation="MTGYRIKQHPILPIPEPEMIEFFWQDKPLSAHKGETISAALFAN
                     GIRTFGHHHKDGSPLGIFCANGQCAQCMVIADGKPLKSCMEIVTNGMRVYPNDGHPRL
                     PEVSVVPELKPIPEIDIPVLIIGGGPAGLSAAIELGKLGAEVILVDDKHRLGGKLVLQ
                     THRFFGSSKAVYAGTRGIDIATRLENEVRAYPSVQIWPQTTCLAVFSDQKVGVLKDGK
                     HYVLIRPQVLLVATGAREKFLAFKGNTLPGVFGAGAFQTLVNRDLALPAEKLFIVGGG
                     NVGLIAGYHALQAGIGVVGLVEALPQCGGYKVHKDKLVRMGVPIYTSHTILSANGQDH
                     VESVTIARVDEQFQPVPGSERSYACDSVLIAVGLDPVNEFFHKANDFGIPAFVAGDAE
                     EIAEASAAIFSGKIKGLEVAHHLGLNTGEIPAEWYRMGEILKSKPGNVLSEKMPDLPE
                     GVLPVFHCSQEIPCDPCSTLCPQHLIFIDQQDIRSTPKFVQDNFACKACLKCIAGCPG
                     LAITLVDYRSSSERPLVSIPYEFSRESIHIGDQVTLEDTQGDELGTAEVVNIRALKAN
                     DRTIVIQVE"
     gene            100138..>101654
                     /locus_tag="PLW25_00395"
     CDS             100138..>101654
                     /locus_tag="PLW25_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOS52976.1"
                     /translation="MKRILFSLIAALFLLGAAPQPAPTMQADNPNPPTQTVKLIFIHH
                     STGENWLRDDNGGLGMALAQNNYFVSDTNYGWGPDSIGDRTDIPNWTEWFASENTPTI
                     MQAVYNESRQNSEYTRNRPDPGGENTVILFKSCFPNSALEGNPNDPPSEEGDLTVGHA
                     KWVYNQILPYFKSQPDKLFIVITAPPLSDNTYAQNARDFNNWLVNDWLRENNYTLNNV
                     AVYDFYNVLSAPDNHHWYNNGRVEDVINKQRNTNAYPSGDDHPNQRGNRKASEEFVPV
                     LNVFYNRWRASAPAQPPVQEEVQAHTGQDGQSPLQPGSQPEGGGLIDAFEGGTPPGTT
                     GWETYRDETTPSIITCAPDSSMAHSGNASMEMEFFVPADSWVTCMLYNEQPQNWSGQQ
                     GISFFIHAQQAGTVIHVNLYAGDPANQESHVYVLETPPDSGSGWALIEIPWGAFHRVE
                     WEENSGVPFTLTNAVLAIGFGASANPDENNKGTVWIDDLGFLGSQVGGQPTTAAE"
BASE COUNT        24571 a        24621 c        27174 g        24788 t
ORIGIN      
        1 accgtttgct ggttggataa ggaaaaaata acaattaacc cgcttgtgat ttgtggtatg
       61 atgaaattaa tttcaggctt agtgtaacga gatgttcttg tgtatgcatt atcattacct
      121 attcaggaaa tgaagccatt catacaggat tactatgaac caaaaagaaa accctcaaag
      181 cgtgattgat aagtactcaa aacgacagaa atacatgccg tatgttcttg gcggcctcgc
      241 tggtatcctt gccctggcgg gcattttcat catcatcagc gttgccaccg gctcaggcaa
      301 tctatttaag gggatgtttg ccaccaaaac gcccacgcca accgcgacct tcacccccac
      361 cgccactgtg ccatctccca ccccttcgat gacgccgacg atcactgaga caccgacgcc
      421 cgccgcaaca gacacgccat caggtcctca gttctacaca gtcaaacagg gcgatgattg
      481 ctggacgctg gcgaatactt ttggtgttga tctgttagtg ctgctggcga tcaacaattt
      541 tccatccggc acctgccctc tcgttccagg aacggatatt atcatccctg cccccggtca
      601 gactttgcca accagcacgc cgctgccggc tgatattccc cgcggaacag aaatcgagta
      661 caccatccaa ctgggcgaaa ctttgcagga tatcgcctct cgatttaaca gcacgcttga
      721 ggatatcaag acccgcaata atattaaaaa tgaaaattta atctatgttg gtcaggttat
      781 caaggtgcgt gtcaatctgg ttaccgctac accaactgtg gcgccaacca gcacgctttc
      841 actgttcact ttacaaccca cgaaacccgc ggtgacgacg accccgtaaa gagttgctcc
      901 gcgcattgac ctaccagata caaaacgatc ctctcgaaga gacgggggga tcgttatttt
      961 ttgagttgga ttttatttct ggcccatctc gctgtgtccc gcagccaatc aagctcagcg
     1021 gtatcctcgg ttctttttat actgccgagg gcgtttttct ctcccaagtt ggccagcctg
     1081 atgatcacat tgcgcttcaa gccattgtag cgggctcgcg ccagggctgt ttcagcaaag
     1141 cgtgatttaa agtcatccat agatagctca aggaattcca gcatctcttc cacggtcatt
     1201 tgaccggcta tacctatgga actggatgat tggtaccccc gctggttcca ggggcagacc
     1261 atctggcaga tttcacaacc aaagatccag ccgccaaatt tttccatcac ctctggggga
     1321 atcatccctt tattttcaat gttgtggtaa ctgaggcagc ggttggcgtc aatcacccgt
     1381 tcgggcagga tgcagccggt tgggcaagac tcaacacagc gcatacagct gccgcaatga
     1441 tccgctgcaa agggagggtc cgcttgcaat gcgatagcgg tgaagatttc ggcgagcaga
     1501 agattggagc catgttttgg cgaaatcaaa caggaattgc gcccaatcca acccaggccg
     1561 gaccgtactg ccagttcacg ctcaagaaca ggggcgctat cggtatacac tcgcggtgcc
     1621 ggcgcttcgg gattgagcgc tgtgagatac tgcagcaaag gggaagccaa gttgggaata
     1681 aggtgatggt aatccttgcc tgaagcgtat ccactgatca agcccgttgt tttttcttca
     1741 atttccgcgg ggtgctgcag cggataacga taacctaaga gaataatcga tttcaaaccc
     1801 ggaaaaaatt tttctgggtg cagcctgttg gaaacgtgat aagcagacgc gagataggtc
     1861 atgcctgcgt tcaaaccgcg ttccaaccag ttaacataca tatggtgaaa atcgagtgtg
     1921 tctggggtgg tgataccgca aacagtaaag ccaaggctca aggccctggc tttaatttcg
     1981 ctttccagtg aagaaggtat ttgagacact gccttcgggt gcaatcctag ggggtacccc
     2041 acccggcgga tacagtgcca gtgattgtag gcgtgatggt tgcggttggc gcgttgcctg
     2101 tgggagtggg ttctccgacg atgatgctga tgttaaatgg gtctccgaag acataaccgc
     2161 tggagttata cattttccag aagctgccgt aattgccggg ggtggtaggt gcgataaaag
     2221 gcacactgat ttcagcgaaa tcaccaggct tcacctcggc tggcagtctg aacatcaact
     2281 ggctgcccgc tccatataac acaccttcca ggtaaagtac cgagtactcc tttgtccagg
     2341 ttgttttccc ggtattctga agtttccagg tttttacgat gtgttctcca ggttttacca
     2401 cgctgccatc agggtatgtg acatccgtga tgaacaaggc gttatccgcc gttgaagttt
     2461 gtggctgcgt aggaacaaga acggttaccg gcgatgatat cgcggtgagg ggagtggggg
     2521 tctgagggct gggagtggct gtggcggttg gttcaagtgt gggggtggca gttgggttgg
     2581 acgccgcagc ctgagttaat ccagcctgca cagtggcggc gacctcggtc attttctggt
     2641 tagggtcaat ggatggtttt cctttacagg agaccagaag catcccgata acgatcacaa
     2701 tgatgaagtt gaacagcatc cttcttttta acatgaataa cttcctccgc ttagttgaaa
     2761 cacgcgccaa ttgtccgata ccttgcgtat cctgtcaaga gtggannnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnatcca caattcaggg ttctggagca gcatgccatt
     2941 tttacctttc acccgtactt caaaatgcag gtgaggtccg ctggtgttgc cagtcatgcc
     3001 gaccgcgccg atcttttgcc cggctttgac caactcgtta accttgacaa gcgttttgcc
     3061 gagatgagta tagacggtat agagggtatc tccatctaat tccatatcat gccgaaccat
     3121 tactgcgata ccgtaggggt catttctcgc tccctcgcca taaagcaaac cgtaccctga
     3181 gaaaactact gtgccatcgg cagcggccag caccggggtt ttttccggcg ccgggatatc
     3241 caatccgcca tgcacaatgc tctcctgctt tatgaaatac ccatatcggt aatcagccgt
     3301 ccgccaggtg aggatttcat cacggatggg gcgagaaagc caaaaatggt cgtggggggt
     3361 taaggatagc ggagcctgaa aaaagggttt attagaaggg tagtaagggt cttcaggggt
     3421 gtcacccagc cggagcgcat caccctggga agatgaatta acgtttgtgc tgaaaggcag
     3481 gttgtttacc ggcgcggcag cgctgataaa aaagaccgat atgatgaagc ctatgaccgc
     3541 ccatctttga ggaatgcctg aaggtttttt catgggagcg accagtaact ttcgatggta
     3601 gtatcggtaa accgttgcct catggcctca tgccagctaa gcccatcacg caatacgaag
     3661 cggttgatac gggtttcagg gaaataaacc tgccagtttg atccggccgg cagcctttcc
     3721 cagccgtacc ccagggcgaa ggaagtgaaa tcgatccaat acccctgggg gagggttgtg
     3781 gccaggccgc cattttcata ggcagaagag gaaccctgaa tgcgggcatt aaaatcccag
     3841 accgggttct ggataggtac accgcaggtt ccatcttgct gcatgcactt aagccacaca
     3901 cgccagtagg tgcgcccaag gatatcctca cggctgacag ccatccaacc agaatcgagc
     3961 gggtccaggt ttaccgtgat ggctcttccg gtatatcccc agtcttcgcg caaccctgat
     4021 gaagagtaag caggaataga aatgctggcg ctctccagta ttttgagaaa atcccaaccc
     4081 aacttagagc gcaaggcagc gcgcatactg ttaaagcttt cgttgactgt atcgatgagg
     4141 tatggttcag gcgcggtgac atctggcagg tataccattt cataacgacc agaactgaca
     4201 tcttgtgtgc ttatctcagg ctgccataat ctcgtttcgc tggcagggga gtgttgagag
     4261 aggtatttct taagaggaag cgctgatttt gctggcagcc aactgatgga cagtacctca
     4321 tttttctgat ggatgggagg cagcagcagg aggttgggtt cagtgctgta ggcgacaagg
     4381 tcgttgctgt tggcggaatg aaccacagcc agcacgcctt gctcttcatc catccacact
     4441 ggatgcgaac ctgaccccat ggtaacaggc ctcgtttgtt ccgactcgag gttccaggct
     4501 tcgatttctt cgaattcatc tgtccttann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnngt ttgttccgac tcgaggttcc aggcttcgat ttcttcgaat tcatctgtcc
     4681 ttacccaggc cagcgaatga ccatcgcctg accatacagg gtgagtgtaa tccgcatgtt
     4741 ctgagcgcgc cacgagatta aagcgttgct gaggattatc caggtttgcc agccataact
     4801 ccaacctgcc attgcggtca gtgacgaagg cgatctcgcg ccctgagggc gaccaggctg
     4861 ggtcaaaatt atttccgcca ttgtcggtca gcctgtgcgg cgcgacagat tcatccatta
     4921 cggaacggat gaccaggttg agtttcccat ccatcaccac ctcgtaagcc acccagcgtc
     4981 catctggcga ccaggttggg gagcaggcgt aggtggttgc gttggtgagg ttggttttct
     5041 ctccggtagg caggtctagg atgaaaatat cccagtggcc aaattcatcc gcgcaaaaag
     5101 caagttttgt gccgtccggg ctgatggccg ggtctgcgtg gtcccattca tcatccgtta
     5161 tctgggtcag ggcctggttc agggggtgat aggctgcgag ttgagcgaag ccgccgatct
     5221 ttacagcgag gatgatcaag ccgctggttg ccgggttttc agaggttgca accgacccgg
     5281 gcgaggtggt ggggatgaga gttggggttg atagcagcgg cgaggcggtc tcagagggtt
     5341 ccggcaggct gacgactgtg ccctcgacgt gggcagcgat ttcaggcgcg gcaggttcag
     5401 gcttgcaggc tgatagcgat accacgagga atggtatcag caagattaag cgtgtgcaca
     5461 gacgggcgct ggtgttcatc ttactatcaa gttttcttcc tctgttcctc aggagatcgc
     5521 gctcagggtt cctgttccca aagaatgaca gggttaaaag attggaatct ccctgatgat
     5581 gaccagatgc aatatctgta ccgttcttca atagtggcat cgggtgagta catggccagt
     5641 gttccaaatt gtgcacctgc tggcccgacc ctcgcttcaa gatgaagatg ctctgctgcc
     5701 gagttgccag tattgccagc ggcgccgatc acttgcccgc aattgaccac atctccggct
     5761 cgcacggatg gtttttccaa caagtgcgcg taaagcacgt atactgattt gcggctacca
     5821 tcccattcaa tcggcgtcat gtcagcgaac aatgggtctg aatcacatgg gcgcacaaga
     5881 tccaaattct cctgtggtat cgcggtgggg ataggcaatg cgcttaccca ttcagcggat
     5941 aatacgttga gtggtgtttc gatgagaaca gcatttccgt acgggaaacg atcaacgatt
     6001 accaggttga cgataccggg aaagatggat tgcaccgggt gaccaatcat cgatggcagt
     6061 tctttgaaag tgaaaaaagc caggtcgatc gcggggtgtc catcatcttt atatggggca
     6121 ggaggggtga agccttgaga cataatttta tgcagatcac tgagttcaat gtccttcagc
     6181 ggcgagcagg cttttatcgc gggctgcagc gtggccgtga aagcaggggt tggattttgt
     6241 gtggctgtgt gggaaggaga gggagttgtt gtggcatgaa aggttggcgt cgtcatgacc
     6301 ggtgttgggg ttggtggctg aggaaaggaa ctagaacaac ccgtggctac cagcacgaaa
     6361 cttaaggtca ggcaaaggat gacagcgcgc tgctttttca tattcatctt ctactgggag
     6421 cccacttcta ctggtacctt ttttgagtcc gaaaactcgt tgtcattggg gaagacaacc
     6481 gcctgtaaaa ataaacgatc gttgggactg tgttgaccca ggtgcaaggt aaacgaaact
     6541 agcgggttga atgtgccgat gatggtcgag cgcgcgatct ccatttgctt ttcgtcaagc
     6601 aagctgagtt caagagtagg gcgacattcc acgttattca aaaccagtgt gacacgcacg
     6661 cgttgattgt ctgggagtaa ttttgcctga agacgatcga tttgtggcgg cgctgtcacc
     6721 tttggggagt tgtggggagt attcatcagc ctcatccatt tagatggtga ttttagatgg
     6781 tgattttccg cgattccatg taaaaacgtt tttcgtgtgc ttcacccatg gaaaaggttt
     6841 tcctcggcag cgcgccatca agcatcactg tgcggaacag ttcatctttt gacattcccg
     6901 gtaagtaaat gcctgtgttg gcgggtttca tccccaactc gcaaagggct tcatctccat
     6961 gcacatagtc tatactggct acggatccat ccgcaacgag ctgatccaag atggtctgca
     7021 gcgtacccac caccaggtta gcggtagggc tggtgatact accggccaca agtccccgcg
     7081 ttgagaccaa accaaaccgc tggcattcaa ccgagggttg ttttaccttt gtggtcaatt
     7141 cggtaaaaga acttactggt tccaatgtta gcgccccatc atatatgccc ttaaggactt
     7201 gttcgagatt gactttactg ttgaaaagca cgcggtgaat cggctcgaat gccagcccgg
     7261 cgtcatggat attttccacc tccacaaggg cgaagcgcgc agggtggtcc atgcttacgc
     7321 tgcttttcag ctcttcccaa atcgcttttg cggttgccag ggagtggttg ccatcaccta
     7381 cagcgaacaa caatacgcct ttatcgggag aaacccggta tttcgcgtaa aaggtctgtg
     7441 gattagccag cgcggcgagg ttttggatga ttgatttttc cagcgcggaa tcatttacca
     7501 gcctgctgcg taaatgcccg ccgcgctgca tcagatcaaa atcataagcc agcggtaaac
     7561 gctggctggc ctggacgaca ggaccgatga ccctgtcttc tgggtcatca atgagaacga
     7621 cgatgtgcgg cagctccagg ggggcgttct tgcggatacg gatacgagga ggaagacggt
     7681 caaggatcgt gccttcagtg gcacggatca aactttgaga acctttcgag aaatcgtaat
     7741 gctccaggtc cagggccagc attaacccgc gccgcgtttt taccccgatg gtacgctcaa
     7801 cgtagatgat attttgttga ggtcttaaca agccatcgct gaggtattgt tgcattttcg
     7861 catgcgtact ttgaacgcgt tctgtttctt tgtcggtgcc gagatagact tcaggcaaaa
     7921 taaggtgaaa agttgaaggg caatcaccta cgtttttcgc gacctgttcc cagtattcag
     7981 gttgtgaagt atattgatca caggcgatca cggcccactt cgttaggtca attttttcca
     8041 ccggcaggta aatttctgga acttggacac caatttcagg ataattcctc atcttttcag
     8101 ggctcctctt tttaatttta ccagagcggc atatcataaa aaaacctccc gttgccgatg
     8161 ggaggttgtt aagtatgtgt aaagcgggct acatgctgtg catgaaagcc acgcccaaag
     8221 cgccaggccc agcatgggtt cccagcactg gcgagacggt gctcaacact gcgtctgtga
     8281 ccaggctgga ggggaaacgt ttgattgctc tttctaacag cgcctggcct tctttttcag
     8341 cgttggcatg aaccacgcat aaacgcaccg gaccctgtgc tgttccgacc cgttgttcaa
     8401 ccagatcaag caggcgctca gtggctttac caatggtcct gacgcgttca accgcttcaa
     8461 tccgcccatc gcgcagttcg aggatgggtt tcatgttcaa catagtgccc aaaaaagctt
     8521 gcgcgccgcc aatgcgcccg ccgcgatgga ggaattccag cgtgttcaat acaaagtaca
     8581 cacctgtatt ggcttttgct ttttccgcaa ctgccttgca ttcttcgagg gtggcgccaa
     8641 tccctgccat gcgggcagcg ctgagcacct ggaaccccat ggccatgctg gtgctgaggg
     8701 agtcgaccag ttctattcta gcgcccgaaa aagcattctt cgcctgaatg gctgaatcga
     8761 gtgtgccaga gagtttgctg gagatatgga tactgaggat gtcatatcct tcttctatca
     8821 gcctgccgta catcgcttta aattcttcag gggtaacctg cgaggtcgac ggcatgacat
     8881 ccgcaacagc caaacgttca taaaattcct tcgcctggat atcaacgcca tcccgaagga
     8941 tttcgtttcc ccagatcacc tgtaagggaa cggatacaat cgggttgcca tcccataatt
     9001 cggggggaat attggcggtg ctgtctgtaa cgaccatcac tttattcatg aaaatacctc
     9061 caattactcg tccttggttc tttgccgtat ataaccccgc aacgaaaaaa ccgatgcacg
     9121 caaatggggt ggtttttcga agaagtgcat cttgaccaat aggtgcttca agactaaaat
     9181 ccctgctgaa tttccatcat gggacgggtg gacgaccagg gcgtattcga ttgtttgaaa
     9241 atctgaccgg caggctcaac caaatattca cccagctgcg gcgaaaaggc aaactttctg
     9301 ctgctgatgt ggatgcaatg atgcgcgagg tgcgtctggc gctccttgag gcggacgtta
     9361 actacacggt agtaaaggac ttcaccaccc gtgtgcgaga acgggcgatt ggcgccgagg
     9421 taagtaaggc actcaacccc gcgcaacagg tcataaaaat cgtgcatgag gaattgatcc
     9481 atacgctggg tgaacctgcc cggttgaacc tcagcggggt gaaaccttac gccatcatgc
     9541 tggttggttt gcagggatca ggcaaaacca cagccgctgc caaattggcg aaaaggctgc
     9601 gctcggatgg agagcgggta ttgttggtgg caggcgatcc ttaccgcccg gcggcggtga
     9661 aacaacttca aaccctgggc gagaaaattg gcgtcgaagt tgtcgcggat tcaactctca
     9721 agccacctgc gctggctgcg cacgcctttg ataaggcgca aaaaggcgga tattctgtcg
     9781 tcattattga tactgccgga cgctcgcagc tagaccagac attaatggac gaattacaat
     9841 ccattgccaa aaaagtcaga ctggtagaaa ccctgctggt tgttgatgcg atgatcggac
     9901 aggaagcgct gcatgtggct gaaggctttc gcgatacggt aaatattacc gggctggtct
     9961 tcaccaagat tgatggagac gcgcgtgggg gcgcagcgat ttccgttcgc aaggtcacag
    10021 gggtgccgat caaattcctg ggaacaggcg aaggcatcga cgcggtggaa gtgtttgatc
    10081 ccgccagatt ggcgtcacga attctgggaa tgggtgatat gattgggtta attgagaaag
    10141 cggaagccgc tttcgacgag aaaacccaaa aagagcaggc agaacgggtt ctcaaaggtc
    10201 agttcaatct tgaagatttt gctgaccaat tgaagcaact gcgcaagatg ggtccgatct
    10261 ctcaggtgat ggatatgctg cccggcgggt tagggcaggc ttcgcggaat atcccaccag
    10321 aagaaaccga gagaaacttg aaacgcaccg aggccattat cgcttcgatg accaaacagg
    10381 aaaaacggga ccccgacctg ttgaacgcct ctcgccgccg gcgaattgcc agtggatcag
    10441 ggacaaacgt gatggaagtg aaccgtctga tcaaacagtt tagggatgtg cagcggttga
    10501 tgaagacgat gcaaaaaaca ggcgggaagg gactttcccg attgttcaag tgatcaaaaa
    10561 tgaaacagcg gtaagcgctt ccttcagcgc ccctcgccaa agcggaattt ttggattcac
    10621 atcatatttg atcatttttt gagaaacagg agataagcaa atggtaagaa ttcgtttacg
    10681 caggacaggt caaaggcacc agcccagcta ccgcatcatc gtggcagata aagaaagccc
    10741 gcgggacggt cgtttccttg aaatcatcgg gcactacatt cctacgactg accccttcac
    10801 tttcgaagtt caggaagatc gggtgtacga gtggatgaaa aatggcgccc tccccagtga
    10861 ttcggtctta aagctcttta aaatcgttgg tgtgctggaa cgttatgaaa gatttaaaaa
    10921 aggagaatct ctcgagaccc ttctggcaga agccaaaaaa gcgacagaag agcggaacgc
    10981 caagattaaa gcctgaacag gatcatgatg cagccaacca acagacccac acaacgctat
    11041 tcgcgcaatg tcaaccagga aagtcacatc aaagagctgg tggaatacat cgcgctttct
    11101 cttgtagatg atccttccca ggtggtcgtc actgaatacc gctcaggagg acgggaaaac
    11161 ctggagctgc acgttgccag ggaagacatg ggccgggtca tcggcaaagg cggcaaaatt
    11221 gccaacgcca tacgggtgtt attacgcgtt gccgcagccc gtgaaggcaa acaggtcaac
    11281 ctggacgtcg ctgaaccgag atgaccaatc gcaagtcaac gggtcctcaa ttaaagacac
    11341 aaccaggctc gcctcaaccc ggcgagcctg tctacttgat gattggcaag cttcgccgcc
    11401 cacacggggt aaagggagaa ctatggcttg aactgaccac agaacaccct gaaagaatcc
    11461 gcactggtgt aacgattttg attggcgatg aaaagacgga agtgaaggtt gagaatatcc
    11521 gtgcggctga taagctatgg ttgatcagtt ttgccggcta ccaggattgc caggaggttg
    11581 agggatttcg taaccagtgg gtgttcatcg aagcgaaaca ggctgctcca ctgccggcgg
    11641 gacggtacta tcatcacgag attatcggta tgaaggtctt tactgaaacg catgacctta
    11701 taggagtagt cgacgaaatt ctggttacag gcgcaaatga tgtttatgtg gtaaaaaatg
    11761 ataaaggcac cgagaccttg gtgccggcaa taaaatcggt aatcctggaa attgacaaag
    11821 agatgggccg catgatcgta cgccagcagg agtggttata ggaggctgag ttggagccgg
    11881 acaaatactg ggtaggtttc aacctggtca agggtattgg agctgttaga ttcaaacagg
    11941 tcctggattt ctttggttcg gctgaaaccg cctggttcgc cccggtttct ggattgcagg
    12001 ctgccggttt accccaacga gtgatagaga attttttgct tgtcaaatct caggtggacc
    12061 tggagcgggt gatggaaagg attcaccatt ccggcaccag ggtgatcacc tggaatgatc
    12121 aagattatcc ccgccgctta aaagaaatag atcagtctcc tcctgtgttg ttcatcagag
    12181 gctccatcaa tgtggaagat gattgggcag tggcagtggt aggcactcgc cgggttacac
    12241 cttatggaag gcaggtcgcg aatgaaattg ccaccttcct gggtcagaat ggcgtgacag
    12301 tcgtcagcgg gttggcacgc ggagtggatg cgatcgccca tcaatctgcg cttgcggcgg
    12361 gtgggcgcac aatcgcggtg ttgggtagtg gtgtggatgt tatctatccg cccgagcatc
    12421 gcaagctggc ggtcgagatc atcgctcaag gcgcgctggt cagtgattat cccctgggca
    12481 cctcaccaga tggggtgaat ttcccgccgc gcaaccgcat catttcgggt ttgtccctgg
    12541 cgactgtagt ggtggaagca ggagagaaaa gcggcgcgct catcactgct gaatttgccg
    12601 ttgaacaggg tcgagatgtt tttgctgttc ccggaagtat tctggcgccg caaagcgcag
    12661 gaaccaatca attgattgag caaggggccc ggccgttgtt gaaaatggct gaaatactcg
    12721 aagtgcttaa actggaacag ataccagaaa agcagcaggc gcgaaaactt aacccgctga
    12781 accaggcgga gcagaacctg ttgcagcacc tctcgacaga accgctgcat attgaccaac
    12841 tctgcgagtt gacgggcttg ccgattcatg atgtatcagc aactctcagc atgatggagt
    12901 taaagggatt catttgccag gtaggcggca tgaattatgt ggcgatgcgc gaaaatcgag
    12961 gtttgtacaa ggtgaataaa tgaagataga acattattac gcggtgatca tggcaggcgg
    13021 cggaggtacc aggctttggc ccttatcccg ccatgactct ccgaaacaga cgctaccaat
    13081 ctttgagggc aagtcgcttt tccaattggc ggttgaacgc ctgcagggat atttaccggt
    13141 aaatcagatc tatgtagtca cttctgctga cctgatggaa cagctgcacg cgcaggttcc
    13201 cgaactgctg accagcaatt ttctcattga accacaaccg cgtggaacag cagccgtggt
    13261 cgccctggcc gccgcggcaa ttttagaaag agaccctaac ggggtactgg cggtattaac
    13321 tgcggatcac ctgatgcctg ataaagcaga attccaggcc tgtctgcaag ccgcggcaga
    13381 actggcagaa cagggatata ttgttaccat gggcatcaaa cctgcttcgc ctgcaaccgg
    13441 ttatggctac attgaacgcg gggctgagct tggggtgtat tcaggcaggg tgggctatga
    13501 agtattgaag tttattgaaa agccgcatca atccaaagcc aacgaattga tcaagcagga
    13561 acgctactcc tggaattctg ggatgttcat ctgccaggct caggtggtac tgggagaatt
    13621 tgagcgttac atgccggaac tgtacacggt catacaatcg ctgcaaccat ttctcgcgag
    13681 cgaccatctg gctgcgcctt ttgcggtaaa gtggttgaag attcaacctg aaacaatcga
    13741 ttatggcatc atggaaaaga cctcgctggc agcggttctt cctttatcga ccctggaatg
    13801 gatcgacgtg ggttcctggg attcgttctt tgaggtgttt aaacccgatg agaatggaaa
    13861 tatcatcctg ggagttaaat atgaaggaat tgacacggct gattcactga tcctatcgac
    13921 agatacggaa agaccggtgg taacaatggg gatgaagaat atgatcgtca tccaagcccc
    13981 tgatgtactg atgatatgcc cgcgagggga aagtcagcgg gtgaaggaac tggtaaacta
    14041 cctgaaagcc agcgattata cccttatctt ataggagagc cgactgtgga agcctattgc
    14101 gtcaagtgta aacaaaaaag agagattgaa tctcccaacg cagagttcaa tgcgctcggg
    14161 acgcctgtga cccgcggaaa gtgccctgtg tgcaacacca gcctgtatcg gataggagaa
    14221 acgcctgctc acgccggcct tgaacgacca aagagactgc acgccaaacc gcgcagcggc
    14281 aaactggtga ttgtagaatc tcccgcaaaa gcgaaaacgg tggggcggtt tttgggcaag
    14341 gactacacgg tgcgggcttc cgtggggcat gtgcgcgatc tgctgcgttc agaactttct
    14401 gtagacgttg aaaacaactt tttgccaaaa taccgggttc cgaacgaaaa acgaccggtc
    14461 gtgaaagaac tgaaagcgct ggccaaagag gcggcagaaa tctacctggc aacagaccct
    14521 gaccgggagg gagaagccat tgcctggcac ctgttagaag ctgcccagat cgaaccaaaa
    14581 ctctccagga gagtggtttt tcatgaaata actgaccacg cgataaacga ggcgttttca
    14641 cagccgcgcg aaatcaatat ggacctggtc gacgcccagc aggcgcgccg catccttgat
    14701 cgattggttg gctattccat cagcccggtg ttgtgggaga aggtgcgcag ccgcttatct
    14761 gctggcaggg tccaatcagt ggcgctgcgg ctcattgtgg aacgcgagcg ggagatagag
    14821 gtgtttgtcc cggtagaata ctggtcgatc catgccgaac tcacccccga gggcaaaaaa
    14881 accagttaca tgaccaaact ggcaaagatc gatgaggttg accccgacct gggcaacaag
    14941 gaggttgtgg atggttacct ggcggatatc gaggcatcac cctttgcggt aacccgcatt
    15001 aaaaagagcg aacgccgccg aaaaccatcg gcgcctttta tcaccagcac cctgcagcag
    15061 gaagcttcca ggaggttagg ctacaccgcc aggcgcacca tgatgattgc ccaacaactt
    15121 tatgaggggc tcgaggttgg cgagggcggc gcaactggcc tcattaccta tatgcgcacc
    15181 gattcaacca atgtggcaga agtggctcaa gaagaagccc gctcgtttat ccaacagacc
    15241 tatggggctg gctttctgcc agaaaagcca ccgcaatata aaaccagagc tgtttcagcc
    15301 caggaagcgc acgaagccat cagaccaact tcggtactgc gccaacctga aaagataaag
    15361 gagttccttt cttccgaaca gtccaagcta taccagctga tctggcagcg cttcgtagca
    15421 tcgcaaatgg aatccgccgt ttatgacacg atctctgtag aggtgaccgc cactggtaaa
    15481 gatcatcaat atcttttcag ggcatcaggc tctaccatta aattcccggg ttttttgatc
    15541 gtgtatgaag aaacagttga tgaggacctg aaaacagaag aaacagaaaa tgtgcgcatt
    15601 ccagccgggt tggtggaagg tcaggcgcaa caccttaaaa gacttatacc cgaacaacac
    15661 tttacccaac caccccctcg ctatactgaa gcctcgctgg tacaggttct ggaggaatat
    15721 ggcattggac gcccttcaac ttacgccccg atcctttcta ccattcagca gcggggttac
    15781 gtgcttcgcg aggcaaaaag gctttcgcct actgagacgg gaattttagt caatgacctc
    15841 gtggtcgagc atttcccgga cattgtcgat ctgaacttta ccgccggtat ggaagcccac
    15901 ctggaccagg tggccgcggg taaagaagac tggcataaag tgttggcgga tttttacact
    15961 acatttgaac ctcaggtcaa gagggcgcag gcggagatgc ctgttaccaa gacagagccc
    16021 gaaaaaattg gccgcgcctg cccgaattgc gggcacgacc tggtcatccg ctgggggcga
    16081 tatggcaagt tcatcagttg ctccaacttc ccggcttgcc gccatacaga agccctgctg
    16141 gagaaaattg gcgtgacctg cccgaaggac gacggcgacg tggtgctgcg caagacccga
    16201 aaggggagaa tcttttttgc ctgcagcaac taccctaatt gcgacttcac cagttggaaa
    16261 agaccgttac ctgtaccctg tccaaaatgt ggcgggtgga tggtggtagc gaacaagcgt
    16321 gaagcccaat gcatggtttg cgaggagtct gttctgatcg aagagctcgg ggtgccaatt
    16381 cccgattagg cataataatt gcaacgtagt taacgagata actggatcaa ctactatctc
    16441 caggagttaa ctatggataa gttgcgtgat gtttttttca agttgatcga taaaccacta
    16501 tatacagcca tcgccggctt cgtgatcggc ctgatcatcg gcttgcccat attgggttgg
    16561 tggctgtggc cagtcaagtg gaaagatgcg gatgccagct ttttacgcca ggacctgaag
    16621 aaccagtacc tctgtatggt tgtggattca tacagtgtga accatgatgc cgcactggct
    16681 ggcgcgcgca taaattcgct tggcgtggat attaagaaaa acccattcct gttcgataac
    16741 ctggctgcag gcgggtgcaa ttacgcacca accgataagg taatccagag cctgaaaacc
    16801 gctttggtga cgggtgagca ggttgtgccg gagacagaag caaacaatca accggatggc
    16861 gatcaggctg agatcaaggc aacagaacca ttggcgactg cagaaatcgc gggttcgacg
    16921 gccattccct tacccgggaa taatgcgcca aaagagaaaa aaggcggaag tgtgttgctt
    16981 ttatcccttc tttgcgtggt tttcctgctt atcggtgggg cattgatcta catctttttc
    17041 atccgcaatc gccgcacatc agaagaaatt cctcacgcat gggatgaaga agaaagattc
    17101 gctcaagatg agccctacga cgatgcgcct gaataccccg ctgctgcaac accgccgcca
    17161 ccaacggcca ttcatcacga atcggttgat acgccaccga tggattactt cagcgaaccc
    17221 gaggaaatgg acgatgtcga cgagatggaa attcctgaca tccttgaggg ggctcaggat
    17281 gttgccaaaa cccctccatt gaagagccct ggagtgcgcg ctacgcttcc tcatcattct
    17341 gacccggtgg ctcactttat gaccacctat gtgatcggcg atgacctgta cgatgacagt
    17401 ttcagtattg acgcaccgaa cggctcattc ctgggtgaat gtggtgtagg cattctggat
    17461 acgatcggcg tgggagaccc gaagaaggta accgcttttg aagtatggct ttttgataag
    17521 aatgacatcc agaccgttac caaggtgttg ctcagcgctc atgccatgaa cgatgcggga
    17581 gtgaagcagc gcctggcttc gaaaggtgag ttggtccaga ttgaatccgg tcagcaatta
    17641 ctattggaaa cggccacgct ccagcttgag gcccgggtgg ttgaaatggt ttatgggcag
    17701 ggtgcgctgc ctgccagtag tttcttccag cgcatgacgc tggagctttc ggtttggccg
    17761 aagtaatacc agacgattga agaataatcc gggagaaccc cggattattt tattttactt
    17821 ttagaatacg gaaatggagt tctccggcag gcgtgttggc ggttatcctg tcgccttctt
    17881 ggtgccctaa gagcgcctga ccgattggcg aatcataaga gatctttccg cgccgtggat
    17941 ccgcttcttg aggaccgacg atatggaagg tttcttcttc ttcctggtcc tcctggatgg
    18001 tcacggtcga gccgatttga attttgcctg tgtggctggg gccatcttca atgagcacga
    18061 cgtcgcgcaa tatctgcgtc agttcctgga tacggccctc aacaaacgcc tgttcttctt
    18121 tagccgccgc ataatcagca ttttcggaga ggtcacccat ctgaatagca gctcgcagcc
    18181 ttttcgccag ttcctgcctg attgggcctt caaggtgttt cagttcagcg cgcaacttct
    18241 cagcgccttc tcgggtaaga tatgaaggtt gattcatgta gactcccttg ggtgcgatta
    18301 taacttacgg cagcagtgag ggatcgaata acttttgata ttcgtcaaca gcaaaacggt
    18361 ctgtcattcc tgccaggtaa tcgcaaatgg tcttttcttt tcctttgatc tcgagctgat
    18421 cctggaaatg tattgggagc atgttgggct ccgaaacata ggcgtggaac aattccgata
    18481 aaattcgctc cgccttcttt tgcatgcgca cgacccggta atgccgatac atgttcttat
    18541 agaggaaatc tttaagttcg cggtttcgtg cgcgcatcac gtcatcaaac cctaccacgt
    18601 tttgagcgtg cgactgcaag tcagcgagtg ttttaacacc acttcgctga aggcgctcat
    18661 tggattcact gatcatgcta tccacttcca ggccgatcag ctcgcggatg atactgtggc
    18721 gggtcaggtc atccaactgg cgggaatgaa tattgagcct ttccattacc atgttccaca
    18781 ggtctatctc ggataacata tctgtggtga tcatccccga acgcaaccca tcatccaggt
    18841 cgtgagcggt gtaagccagc tcatcggcga cattggtgat ttgagcttcc aggtgaccgc
    18901 gcaaatgcgg gtcataatcc agcgcgtcgg tcttatcata ttcggtttca tgcttgacga
    18961 tcccttcgag cacctcccag gaaaggttga gtcccggaaa ctgcgcgtag cgatgctcta
    19021 attccgtcac gatgcggtaa gactggtgat tatggttgaa cccgccatac ccttccatca
    19081 attggttcag ggtggtttcc ccggagtgtc caaaaggcgg gtgaccgagg tcgtgcgcca
    19141 tgcagatggt ttccaccagg tcttcgtttg cacccatcgc ccgggcgatt gtgcgaccga
    19201 tttgcgccac ttccagcgta tgggtcaggc gggtgcggta ataatcgccc tcgtaattaa
    19261 tgaacacctg ggttttgtat tccaacctgc ggaaagaggt tgtgtgtaga acccggtcac
    19321 ggtctctttg gaaacaggta cggtacagcg gctcgttttc agggtatttt ctgcctttgg
    19381 agcggctggc gtgaacggcg taagtcgcca gggtttcttc ttcaattttt tccagataag
    19441 cgcggtcgaa tttcatgtca ctatcctttt ttatccaggg ttatacaggt gcccactttt
    19501 ctcccggtca acgcctggaa cacgttgccc tcatcaagac cggaaaagat caatacctgc
    19561 tgacagtgca ggttcgtcac aaggttcatc atgttgtgaa ccttactacg catgccgcct
    19621 gtcacgtccg ggctctctga tgattcaagg tgaggatcca ccatggcaag gtttcgcggc
    19681 gtgatggcat ccagcagttc tttcctttta ggaaaatcca tccaaacacc cttttcgata
    19741 ccggcgagca aaacgcgagc aggcttgaag atcgctgcca gaaattcaaa ttgctcttca
    19801 gtggatagaa ttgtgccgcc gatggtttga tcaaagacca catcaccgta gaccactggc
    19861 agaatgccat tatcgagaca agcagcgatt tgcgtcgtgt cccagtgggt gattttgtga
    19921 gaagaggtga tgacctgggc gcttggtgga aaagccatcg cgtgaacgcc actttcatgt
    19981 aaaacctcca tcacgatggt attgagagcc cgagcttcat gccaaacctc taccagaccg
    20041 cgccactgtg caaaagtggc aacgccatca cgtgtttggt attttttcgc ggggatatgc
    20101 ccaaaggaac ctgaaccatg ccctaaaagt aaccgaactc caggctgatc ggtcaatgcc
    20161 ctggcaattt catccgccag gcgcttcaac acatctgacc tggcttggtg cgggatgttt
    20221 ttatttgtga tgagagagcc accgagcttg aggaaaaaga ggggagttgt catcaggcac
    20281 ctcgccctgg tgcgagggtg gtggtgatgg tgcgcacggc tccctgctgc tctaacgccc
    20341 tggaaatact atctgcttgc gtatggtgca caagcgcgat gatattcccg ccaccgcccc
    20401 caccggacaa ctttgcccca agcgcccctg cttgaagcgc gcagttgacc aaatcatcca
    20461 gcattggagt ggagactccc agcgcttgta gaagttcatg gtttctgctg agcagctcgc
    20521 catgctggtt cagatcattt ttttcgaggt tctctttcac ctcccgcgat aggtcagcga
    20581 tgttggcaaa gatggcgtcg taacgttttt tttcggcaag ccagcgttcg cgaacgcggg
    20641 ccacggtctc tgccgtcagg ctggcagcgc cagaatcagc aatcaccagc gtgaagggtt
    20701 ggcgaatctt gaggtgttca atcggtttcc ctttaacaaa gtaaaccggt acctggtagg
    20761 tgacgacggt gttgtcgatg ccggaaggcg taccgtgatg cagtttctca atttcaaagg
    20821 cgacttgatt gaccagttca tcggataagg ggctgccaag gaaggcagac aacgcccgcg
    20881 cgagcgatac ggttaccgaa gcgctggagc ccagtccagt ggcgattggg atggtggtgt
    20941 tgatgcgtat ctcacaagcc tgaagcctgg taacccccat tttttgcatc accagggcgg
    21001 tggttgcttt taatgggtgg ttgtacgcga gagattcaag gtcactatcg agaccaatac
    21061 agggggctat cattctcact ttgccttcag gcgcggttgg ctgggcaagg atggtggttt
    21121 tcgtgttaag ctgaaataca ggcagggcga tggcgggagc gccataaacg acagcgtgct
    21181 caccacaaag gataaccttg cccggggatg atgatgtgat ggctggcaca gctcacctaa
    21241 aaaaggtaaa aaatcaccat cactgccacc ccccacaaga gcacggtcaa ctggattgga
    21301 cggtctttga gcaagatttc ttcaggctca ccacccgcgt tgtgtttgtt gatgaggaag
    21361 agataacgga aaataccata aagcacaaac gggatcgtca gcatcatcga atggttttca
    21421 ggaaggtttg gggcggagaa agtgtaaaga gaataagaga tgagagtagt agcggaaaca
    21481 acggtaatca gctggtcaat gaactggata gaatagcctg aaagcacctt acggtgcgat
    21541 tgagggctgt cattcaccag taccgccagt tctgcgcggc gcttgccgaa accaaggtat
    21601 agcgccagca gggtagtgac gacgtaaagc cagggagaga agcgctgtac ttcaatcacg
    21661 gaaaccccgg cagccacgcg cagcacaaaa caactggcga taaccaacac atcgattatc
    21721 gggatatgct ttaaccagat tgaatagatt agattaagcg taaaatacac cagcgatatg
    21781 aaaaagaaac catgagagag caagaacgct cctgtcagcg agaccagcaa gaggaagatg
    21841 ccggtggcta aggccagacc cactggcaga ttgccggagg cgatggggcg gttcttcttg
    21901 atcgggtgcg cacggtcaga ttcacggtcc agaatatcgt taatcaggta cacgctgctg
    21961 gaaagaaggc aaaagagcac aaatgcagcc agtgtttgca aaaacgcgtg gagattgagc
    22021 aatttgcggt cgaagaccag ggcagcaaaa atgaacgcgt tcttggtcca ttggcggggt
    22081 cgcattgcct taaggatgta cttgagcatg aggataatat atcacaagtc cattgttcgc
    22141 gattagaatt gggttatgca aaaaacgcgt ctggatattc tgatggagtc acgtggtctt
    22201 tcacccagcc gttcacaggc gcaacggctg atcatggctg gcagcgtacg cgtaaatcat
    22261 caggtggtgc tgaagcccgg tactaaagtg gacccggggg atacgattga aatcagcgaa
    22321 tcaccacgct ttgtttcccg cggcggtgaa aagttacttt ctgcgttgca ggcttttcat
    22381 ctgacctctc tggcggacag ggtttgtgtt gatgtgggtg ccagcactgg cgggtttacc
    22441 gattgcctgt tgcagcacgg agcaaaaaaa gtattcgcgg tagatgttgg ctatggcgtg
    22501 ctggattgga aaataaggaa tgacacgcgg gtggtggtga tggagaaaca aaacgcgcgc
    22561 tatatcacca cctttcatga accgatcgac ctggtggtaa ttgacgcctc gttcatctca
    22621 ttaagaacac tgctgccagt tgtgcgccaa tggttgaacg ccccaaaagg ggaggtcgtt
    22681 gcgctgatta aaccgcaatt tgaagccggc cggagcgaaa ctgcccgggg cagcggcgtt
    22741 attcgtgacc ctgaaattca tcgcgcagta ttacgccagg tgctggaatt tgctcaaaag
    22801 gaaggttaca ccgctgaagg tttgatcaga tctcctttaa ccgggccgaa agggaatatt
    22861 gagttcctgg cgcacctgat ctacccaggt gagacgcctg cagatgttga aaggctgatt
    22921 gaggggttac catggaacga gggaaccacc gctgatcaac cctgccaacc ggaacctgaa
    22981 gggaaattga aataaggtat actttattcc ggtatgtcca cagaaagaat tcgcaacttc
    23041 tgtatcatcg cccacgtgga tcatggcaaa tccacgctgg cagaccgcat gctgcaactc
    23101 accggcacca tttcggacag agagatgacg gaacaggtgc tggattcgat ggacctggaa
    23161 cgcgaaaaag gcgtgaccat caaagcatca gcggtgcgca tgaactacgt tcatgctggt
    23221 gagacgtatg aactaaacct gatagatact ccagggcatg ttgatttcaa ttacgaggtg
    23281 agccgggctt tggaggcttg tgaaggggcg ttactggtgg tggacgccac gcagggtatt
    23341 gaagcgcaaa ccctggccaa cctgtacctg gcgattgaat ctaacctgac cattctgccg
    23401 gtgattaata aaattgacct ggtctctgcc agacctgatg aagtcgcgga agatattgag
    23461 tccctgttgg ggttgccggc tgaagagatc atccgagttt ctgccaagga cggtttgaat
    23521 gtggaccagg tgctggatgc cattgtcaca caaattccgc cgccgaaagg ggatgatacc
    23581 gccgcactgc aggcgttgat ttttgacagc cattacgatt cttataaagg agtcgtggca
    23641 tatgtgcgcg tgatgcaggg gatcattaac gcggatacca ctctgcggtt gatggcgaca
    23701 aaagcagacg taaaaccggt tgagataggt gtcttcgctc ctgggatgat acctgtcccg
    23761 ttgttatcgg ctggtgacgt gggctatgtt gccacaggct tgaagaccgt gaaggaatgc
    23821 cgcgtgggag atacgtttac gctctcctca gagccagcgg cggcccccct ccccggttat
    23881 aagcacccca aaccaatggt ctttgccggc atttatccgg ttgaaggcga ggattatgaa
    23941 gcgttaaaag aagcccttga gaggcttcag ctgaacgatg cttcgctggt ctttgagccg
    24001 gaaacttctc aggccttgaa ttttggcttc cgttgcggct ttttgggcct gttccacatg
    24061 gagattattc aggaacgtat cgaacgtgaa tataacctca atattgtggt gaccgctcct
    24121 tctgtagaat atgaagttgt agagattagt ggagaggtct tgcggatttc ctcccccgct
    24181 cgccttccgg atgaggcgct cattgccgag gtccgtgaac catggatgcg cctggaagtc
    24241 atcagcccga ctgagtttta tggcgtgatc atggaactca ccaaaaaacg ccgtgggatt
    24301 ttcgtctctc aagattaccc ttctcccaac cgcgtgcagc tcaactatga tataccacta
    24361 tccgaattga ttgtagattt cttcgatgac cttaagtcac gcacccgcgg ttatgcttca
    24421 atggactacc actttttgga ttaccgacca ggcaacctgg tgaaactcga gatcctggtg
    24481 gatagcgagc ctgtcgacgc gctggcagcg attgttcata aagaagatgc ctaccataaa
    24541 gggcaggcgc tgatcacccg cttgaaggcg ctgattcccc gccagctttt cgatgtggca
    24601 atccaggctt ctgccagcgg acgggtgatt tcacgcgcga atgttaaagc gctgcgcaag
    24661 gatgtactgg cgaaatgtta tggcggggat atcacccgta aaaagaaatt gctcgaaaaa
    24721 cagagaaaag ggaaacgccg catgaaaatg attggtgatg tggaaattcc ccaggaagcc
    24781 tttatggctg tgctgcgttt ggaggatgaa tgagcatggt tcgccccaac tggaaaaagc
    24841 aggctgcgag gtggctgccg gggctcctca tcagcgcgat ggcggtttac gccatcgccc
    24901 atttcacccg cgggcagcac attggcgaag ccttaaagac agtaaaaatc ggctacgttt
    24961 tactgctcat ctttctcacg ttattatcgc tcatcgcgcg cagcatcgcc tggagggcaa
    25021 ttctcggcaa taaaatcacc ctggtcaatt cattttttgg tatttgcgaa gggtattttc
    25081 tcaacaatat cttcccttta cgggtgggag aactgggacg cgcgcttttc gtcggtcgtt
    25141 cttccgggct gggaacgctg cacgtgctat cgaccattct tatcgaacga gcttttgata
    25201 ttgttttcgc cgccagcatt atattgctca cattgcccat ggttgtcggt tcagaatgga
    25261 tcaaaccgat tgctttttct gcctttgtgg tcatcctggt tggcttggtc atcctcttct
    25321 taatttcagt aaagcggcag aaatttcaaa cctggataga aagcaaagaa gtgcgcattg
    25381 gtttcatcaa gaaccgtgtt ttgccgcaag tcagtaaaat cctggatggg ctttccgccc
    25441 tggtgcaccc agggcagttt ttgttaagtg ttttttggat tggcataacg tggttactgt
    25501 gggtgctgct gtattactgg acgattgcgc aactggcacc ttccgccccg ttatggtggg
    25561 gggcttttac atcaggtttg atcgcgctgg gtgttgccat cccatctgcg ccggcttcag
    25621 tgggtgttta tgaagcgacc atcctgggag ccttcgcgct gctgggtatt tccagcggtt
    25681 ccgcggtggc ttatgcgatt gtcttacatc tggtgcagat ttttgtcacc gctgtgtttg
    25741 gtttgtgggg gatggcgcgg gatggtcaaa aactatccac tttacttgat tcgctggtga
    25801 acaaaaaaaa cgtggaaaac cccacgggtt aggagagtga tgaacggttt tacgctgatt
    25861 acaggaggag cagggttcat tggtgccaat tatgcagcca ggtgcctggc tcgtggcgaa
    25921 aaagtggtga tttttgataa cctttcacgc cgcggcgcag aattgaatat tgaatggctg
    25981 agcgaccgtt tcgagcggcg ctcatttgaa ctggtcaaag aagatgtgcg cgatgccgcc
    26041 gccatcaaaa cggctgccag gggagcagac cgcattcttc acctggctgg gcaggtggcg
    26101 gtgaccactt cggtttgcga tccgcgcact gattttgaga tcaatgctgg tggaacattc
    26161 aacgtgcttg aagcagcacg cgcctcaggc aggaaccctg tattcctgta cgcttcgacc
    26221 aacaaggtgt atggggggat ggaggacgag caggttatcg agcaggaaac tcgcctggat
    26281 tatcttgacc acccgcacgg tatcaatgaa aaccaagcac ttgattttca ttcgccctat
    26341 ggttgctcta agggagctgg agatcaatac acccgcgact attttcgaat ttatggcctc
    26401 ccaaccgtcg ttttcaggca atcctgcatc tatggttcgc gccagtttgg tatcgaagac
    26461 cagggctggc tggcatggtt catcatcgca gcggtcactg gcaagccgat caccatttat
    26521 ggcaatggta aacaggttcg cgatgttctg ttcattgatg acctgctgga cgcttacgat
    26581 gccgcgtttt ctcgcgttga ggcgagcagg gggcaggtct ttaatatcgg cggcggcagg
    26641 gagaattcaa tctcggtgtg gcttgagttc cgcccaatcc tggaggaatt actcggtcat
    26701 tctatcaaga cgcgaaaccg tgaatggcgg ccgggagatc agccggttta catctcggat
    26761 atccgtaaag cccggagcgt gctgggatgg gaaccgagag ttactgtaaa acaggggatc
    26821 aggcgattat ttgattgggt aaaggagaac cagaacctgt tttgaagatc ctgatcgtcc
    26881 tcacctatta tcgccctcat atcagtggtc tgaccatcta tgcggagagg atcgcgaaag
    26941 cctttgcccg tcgcggacac caggtgactg ttctaacctc gcggtttgaa aaaaacctgg
    27001 ctgcggatga attagacgaa gatgtgcgca tcatccgcgc gccggtgttg atgcgtttga
    27061 gcaagggtgt gatcatgcct acctttggct ggctggccag caaactggta aaagaacatg
    27121 atgtgatcca gttgcacctg ccacaattcg atgcagccgg agttgcgtta cgcggcaggc
    27181 tgctcaaaaa gcccaccatc atcacctacc attgtgatct tgaattaccg cccggcttat
    27241 ttaaccgggc agccaacctg gcggtgttga cgatgaacga actggctgcc aaatttactc
    27301 accgtatcgt gacctataca gaagattacg ccaggcactc ttggtatacg cgtagatata
    27361 tgaagaaagt gaagattatc cctcctccgg tggtgttgcc gcgcgtttca aaaacagaga
    27421 tcagcgcgtt tcaggctgcg tataacccgc aagcccaacg accggttatt ggcatggctg
    27481 cgcgattcgc aacagagaaa ggcgtggaag tgcttctgcg cgccatcccc cgtgtgatta
    27541 acgaattccc caacgctttg ttccaattcg ttggacctta taaaaatatt attggagagg
    27601 aagggtattt cgcacgcctg gaacctcaaa taaaagaatt tgagcaggca ggtaactgga
    27661 aattcttggg atcgatgtcg cccgcgcaga tggcggcttt ttacgccaac ctggatttgc
    27721 tggtagttcc gagcctgaac tctactgaat cttttggatt ggtgcagatc gaagccatga
    27781 tgaacggcgt acgcagcatt acctctgacc ttcccggggt gcggcagccg gtgaaagtgc
    27841 atcgcatggg atccattttc ccgattggag atgctgatgc gcttgcccgc ggtatcctgc
    27901 acgctttaca aaacccgcag ctcatacagg aagccaacac tgatttttcg gcttacgacc
    27961 ccgatacaac cgcagcagcg tatgaaaggc tttttaacga aatcacggtt gagttacatt
    28021 aaaaccgggc acaatcagct tcggagagac aagtggtgac tgaccagcct gaaaagaaaa
    28081 aacgagccaa caatggaata cgcggcaacc tggtttggga cctgttgctc attgtggtgc
    28141 tgctggcggg cgcaatcttc cgctttaccg gcattcaatg ggatggtaac caacaccttc
    28201 acccggatga acgttttctg accatggttg agaccggcat tgcgtcggtt aaaagcgtct
    28261 ctgactattt tgataccgca acttccagcc tgaacccgaa caatagaggt tatacctttt
    28321 atgtttatgg tacgctgccg ttgttcattg tgcgttacgc tggtgagtgg gttgggaagt
    28381 ccggttatga ccagatcaac gtggttggca gggtgctttc cggggttttt gacctgggca
    28441 ccatcctttt tatttacctg attgccaggc ggctgtaccg ccacgcccgg ctcgggttgc
    28501 tggcagccgg gtttagcgcg ctggcggtat tgcagattca gctctcgcat tatttcacag
    28561 tggataattt tgccaatttt ttcacctatg gcgctctact tactgcggtg catatcgcca
    28621 cgtccagaac cttttcgcct gtcgataagc ttaaggagaa aagatggaaa aacgcgcctg
    28681 actggctgac gactggctgg cgttcagcct ggctttacgc gctttttggt ctgctgtatg
    28741 ggatggcgct ggcttctaag gtgagtattt gggcactggc agcccttctt ccgctggctg
    28801 cctggatcta ttataagaat ctccatgagg aggagaaggc ggctgctctg ccagtgttga
    28861 ttcgcaacct ggcactggca ggggtgattg cctttgtttc cttccgcgtt tttcagcctt
    28921 acgcgtttag cgggccgggc ttttttggct tgaaattgaa ccccaactgg ctgtccaaca
    28981 tgcgggaact ttccacctta atgaaagggg atgtcgacgt gccttacgcc cttcagtggg
    29041 cacgccgacc gctgactttt accattcaga accttgtgtt atgggggttg ggtgctccgc
    29101 tgggcgtact ctcactgggc ggcttggtat ggatgacctg gcgcatcatc agaggcgatt
    29161 ggaaaaagca tattctcatc tggtcatgga cgctgtttgt actggtcacg cagtctatga
    29221 atttcgtgaa aagcatgcgg tacatcattt ccatttaccc ggctctggca ctgatcgccg
    29281 cctggacgat ctttaagctg ctggaaaagg gcagcgcagt tggtcgaaaa cggagattgg
    29341 gtcatttcaa ctggggccga accctttctt ggctgaccct ggtggttacc ttgctggcaa
    29401 ccacgctttg ggcggcagcc ttcagcagta tttacacaaa accagtgacc agggtcgcag
    29461 ccagcgaatg gatctacgaa aatattcccg gcgcgatcaa cctgaaaatc agcgggttag
    29521 atgggcagat attcacccag ccgtacgcgt accagaacac agccgtcatt tcttcagaaa
    29581 acccttatcg ctaccaaatc accgcggtga agaatggaca ggtcaacagc gtcagtgtgc
    29641 agcacgtact gctgcattcc ggtaccacgt tgcaaaatac gcttttgctc tcgatctatg
    29701 aatatggtca gaacggtaag catctggcag gagcaggttt tacgcaagcg gatttttcag
    29761 gcctatctga caattccggt gaccaggcgc tcatccagct gcagacgccg gtgccactgg
    29821 aagctggaag gcattacgaa ttggaatttt ctgtggcgga gcctggcacc tttttgcgca
    29881 tcggcggcag cgtggcaatc aacaccagcc agtggaggat ttatctaccg caaccggttt
    29941 tccaactcac cagcgcttct ccgtatcagc ttttgatcca ccctcgagaa accggtaaac
    30001 tggatgagat tgtcctaaac cgagtggcag accaatcaga agagaaccag gttacgaggg
    30061 tcaaagcttt catatatgcc ccggatgacc cgcaaaccat tttggccagc gcggagctgg
    30121 tgagccgctt cgcgaacgac cctgacctgc gtgggcagcc ttacacgctg aaatttgatg
    30181 aagcgccctt attagagaag aataaaggtt attctctgct gttacaggta gaaggcagcg
    30241 cggttctggg ggtttacaac agcatcaccg ctctggaatc cacctgggat gacgctttgc
    30301 cggtgggttt atatgggtat aaccagttcg gctatatcga tggaatttat ggtaatcacc
    30361 tcaatatgga attgtattgg gatgacaacg ctgaaaagct ggaacgctta acttcgatac
    30421 ttgaccaggc agacgccctc ttcatcagct ccaaccgcca atggggaacc atccctcgag
    30481 taccagagcg ctatccactt accagccagt tctaccgctc gctcatcggc tgccctgagc
    30541 aaaaagacat cttgtggtgt tacgccgtcg ccaaaccggg gatttttcag ggcgagcttg
    30601 ggtataaatt gacggcggtt tttcagaatg accccgcgct tgggcccttt aagatcaacg
    30661 accagcttgc cgaagaagct tttacagttt atgaccatcc taaggtgttg gtttttgaaa
    30721 aaacctcggc ctatgacccg gataagaccg cgaaaactct caaaagcgta gacctgacca
    30781 gcgtggtcca taagactccg cggcaggcaa gccgaatcag tggtaacctg atgttgaccg
    30841 ccaaagaaga ggatattcag actgccggtg gcacctggag agaattattc ccgccggaat
    30901 cacctttaaa ccgacagcca tggctggcgg cggttacctg gtacgtagtg gtcatgctgt
    30961 tgggctggac ggtttaccca ttggtgaggt ttttctttag agggctgctg gacaggggct
    31021 atgggttcag ccggctggtt gggttgctct ttctcgctta tcttacctgg atggcgggct
    31081 caatgggatt gccttccagt cgcgtcacga tcagcctggt gtttttgctt ttattgagca
    31141 caggggttgc agccgcagcc ctcagacggg gagagatctg gcaggagata cgaaagaaca
    31201 gccgccgctt cctcatcatt gaaggggtat ttctggcttt attcgtgctt gacctggcca
    31261 ttcgtatagg taaccctgat ttatggcatc cctggaaagg cggcgaaaaa ccaatggacc
    31321 tgtcgtactt cactgcggtc cttaaaagca ccacttttcc gccttacgat ccctggtatt
    31381 ctggcggtta catcaattat tattactatg gttttgttat tgttgcggtt ttggtgaagt
    31441 ggctgggtat tgaacccgcg attgcctaca acctgatcct ccccactctt ttcgccttta
    31501 ccggaatcgg tgcatacagc gttggctgga acctttttgc cagattaaaa cccgatgatc
    31561 gttcttcacc gactggagag aaacgcagct accaggcggg cctggcggca gcagcggcgg
    31621 tactgattgc cggcaacctg ggcacgctga agatgctgtg gcagggcctc cagcgcctgg
    31681 cagcaccagg tggcatcatc gatggcgccg gattcttcga gaggcttggc tggttctttt
    31741 ccggtgtgat gcggttcgtg aatggcgcgc ggctgcctta tgggtttggg gactggtact
    31801 ggaacccttc tcgagccata ccgggtgatg ttatcaccga gttcccattc tttacattta
    31861 cttacgctga tctgcacgcg catatgatcg cgctgccgat cacgctgctt gtgctgggtt
    31921 gggttgtttc aatcttgttt cgcaactggg aggcccattc ttctgcccgt tttcaaaccg
    31981 cggcaggttt gatcatcacg attctttttg gcggatttac gattgttaca ctgcgcccca
    32041 cgaatacctg ggaccttccc acttatttcc ttttagcggt ttttgtcctt ttctacagtt
    32101 cttaccggaa cgcccaacct cccagcggtt ttctgccagc agccccttgc tggttgcgcc
    32161 gcattcttta ttcaatgatt tttatcggcg tgttggcggc gggggtgatg cttttttatc
    32221 tgcctttcac tgaaaaattc ggccaggctt acggcatgat agatgtatgg aaaggagatc
    32281 attctccgtt gggttcttac ctggttcatt ggggttggca gttgttcctg attgtcagct
    32341 ggtttgcctg ggagacgcgc gattggctgg cgtcgacgcc ggcttccgcc attcgccgca
    32401 tgaagcccta ccttgggtac ctgcaggtta ttggcgtgct ttttaccatg gtggtggtct
    32461 tgctcacgct gctgcgagtg catatcggct ggctggtatg catactgggc gtgtgggcgc
    32521 tcgtgttact gctgcgtcct catcaggcgg atagtaaacg ccttgttctc tttatgaccg
    32581 gcaccgcgct ggttttaacc ctgtttgtag agttgttcgc gctccaaggc gatatagggc
    32641 gcatgaacac agtattcaaa ttttacttcc aggcctggac gctgctggcc atcgccgctt
    32701 cggctgcgct gttgtggctg atccctgctg cgacttctgt gtggaaatct ggcatatcca
    32761 cagcctggca ggttctcacg gttattttga tcgccggttc tctcctttac ccgctgactg
    32821 ccagtatgga taaaatacgt gaccgtatgt ctgcttcagc gccgattggt ttggatggcg
    32881 atgcattcat gcagagcgcc acgtattttg accagggtgt tgaaatggac ctcaatcagg
    32941 attaccaggc ggttcaatgg atgagagaaa acgttcaagg ttctccggtg atcattgaaa
    33001 ccaatacagt tgaatatcgc tggggaaatc gtttcacgat ttatacaggg ttgcccggtg
    33061 tactgggttg gaactggcac cagcgccagc agcgtggctt tttggattat aacgggatta
    33121 acaaccgcct gaacgatatt ccactttttt atcagacgac ggatatcgaa caggcgttgg
    33181 agattcttaa acgctaccag gtaaaataca tcattctggg tcaaatggag cgcgcatatt
    33241 acagcggggc agggttggat aaatttgcaa actatgatgg tctttactgg aaggaagtat
    33301 ttcacgagaa agataccgtc atttatgaga tgattgatta aataggatag cgccaggaat
    33361 gtcacaagcc attaatatct ttgtgtggta cttggtgggg acctttgtag gggtaatcgc
    33421 gctaccgctg gtattccgat ttttcccctc gctaccgagc agaggattcg cgctggctcg
    33481 cccgctggga ttgttaatct ggggattcac cttctggttg cttgcatctt tcggcgtgct
    33541 gcaaaatgac ctgggcgggc aggtgctggc ggttctcatt atgctggcgt tttccttggc
    33601 ggagttaaag ggcacgcgta aagatgcact actcacctgg ctgcggcaga acctgcgaac
    33661 tgtcttgacc attgaagggc tatttctgtt tgtttttctt gcttgggtgc tggtgcgcgg
    33721 gatgaaccca gaagtggcct acacagagaa accgatggag ctggctttta ttaactctat
    33781 tttacgctcc accagttttc cgccacagga cccctggctc tcaggttacg ctatctcgta
    33841 ttactatttt ggttatgtga tggtggccat gttgacgcgt ttcagcgggg tggctgctgc
    33901 aacagcgttc aaccttgcca atgcgagctg gctggcaatg acagctgtgg cattgtacgg
    33961 cattgtgttt gacttatcgc ttgctggcgc ctggaacagt catcatcaaa ccgcaaaaca
    34021 agttgaaaag ttcgcccgca gagctggttt tttagggccg ttgtttgtcc tcctgctgag
    34081 cacttatgaa ggggtgctgg aaattttgca cgccggcggg ttgttttgga aggttgatcc
    34141 ccaggggcaa ttgtcttccc gtttttggaa ctggctttcc atccttgatc tcaaccagcc
    34201 cccgacgcaa ccattcgact ggataccctc gcgtggcggt ggttggttgt ggtggcgtgg
    34261 ttcgcgtgta ttgcaagatc taaacctggg tggacagcat attgaggtaa tcgacgaatt
    34321 tcctttcttt tcgtatttgc tggccgattt gcatccacac gtgctggcga tgccttttac
    34381 cctgctggca atcgcgctgg cgatgaatct gttctttgat ggagggacct acctgcaaca
    34441 aagccgcagg ctgctggatt gggcccggaa cgagaacttt tggctgatcg cgttggtact
    34501 gggcagcctg gggttcatca acacctggga tttccccatc tatgttggct tgttttgcct
    34561 gatctggtct tttaaaagct accgcgcaac gggctggaaa ccaggtcttt tagccgaatt
    34621 tttgaaaaac gggcttgcgc tgggagtggc cggaatggtg ctgtacttgc ctttctacct
    34681 tggtttcggt tcacaggcgg gtggcatcct ccccagcctg gagtttatga ccagaggaat
    34741 tcacttctgg gtcctttttg ctgtgcttct cgtgcccatc atcgcatttt gcctgaacca
    34801 ggcgaaacga aagctgggat ggagtgactt ttggaaaggc gccaggttta cgctggcgct
    34861 gctctctgtt ctttttatct taatggtctt ttacggcgca ttgttactgt cttttcaggc
    34921 gtggggagca gggctgtcgg cttcctcgaa cctattcctc tccgcattgg gagtcaaaat
    34981 gacccagggc ggcgctgcct tcgccggggt gcacgcgggg tatccagcgg gcgaggttct
    35041 aaaagcctca ctgctcaggc gactgcaggc gcctggtact tggttgacgc tggcggcgct
    35101 gttgaccatt actttaagcg ttttgcttaa acgaaaaacg gctcaatcag ttgaaattcc
    35161 agcagaggga gaacatttgc gtatcactaa tggcgcagag gagttgatct tttttctgat
    35221 cctgtttggt ttattgttaa ctatcatccc ggaatttttt taccttcggg accagtttgg
    35281 tacccgcatg aataccatct ttaagtttta ttttcaagcc tgggtatttt ggggcattgc
    35341 tgcggcttct ggcagcgcag tgctttttcg tcagttgcgc ggatggcggg gtagcgtttt
    35401 ccaaattgtg tggatcttgg ttgtggcggg cgggctggct tacccggcaa taatgctggc
    35461 ggataagacc aattcgttct cgatgaaagg atggacattg gatggtaatg cgtacctgca
    35521 aaaattcaac cctgaagaat acgcagccat cggctggttg caagacgcgc cgatgggcgt
    35581 ggttgtcgaa gcggtaggcg gcagttacac cgattacgcg cggatatcca cgcgcaccgg
    35641 cttacccact ctattgggct ggcctgggca tgaaagccag tggcgcggcg gcggggagga
    35701 aatgggctcc aggtttgcag atatccaaca gttgtacgaa acgacggatt ggaatgaggc
    35761 ttggcgcacg ctgcgagact ataatgtgag gtatgtttat atcggcaacc ttgaacgctc
    35821 aacctaccgg gtgtcaccat cgaaattcga tagtcatttg ccgcttgttt tctccaatag
    35881 cagcacgagg atatacgagg tcactgcccc ctctggagaa gcgcagccat gaacggtaat
    35941 cttgattcac gaaaagctgt accgcttgag ttgctggttt acctggtagt gctggttatt
    36001 gccgcagcgc tacgtttttc aaacctgggt ggtttacctc tgactgatca tgaagccagc
    36061 ctggccttgc aggcgctgcg tttgagcaaa ggagagcagg tgctgatctc tggcgagcca
    36121 ggttacatcg cattgaccac tttgtggttt tctctatttg aactctctga ttttaccgcg
    36181 aggttctggc cagcgttggc gggcacactg cttgccctca caccgctgct ctaccgggag
    36241 agggtgggga aagccgccgc gctggtgctg gctggccttg tggcggttga ccccataatg
    36301 atcggcgcat ctcgcagcgc tgaagggagt atgctggctt tattagggtt gctcgcagca
    36361 gtgggttttg ccatgcgctc caagccgctt tggtgcgggt tcgccttggg attcgccctg
    36421 gcaggaggac ctgcgctgtg gccaggagta atctgcctgg cggttttcct gttgatcaac
    36481 tgcaagagcc ttcgccaggt tgacaaccgt tttttggcgc tggcagcaat gaccgcgatg
    36541 gtcaccctgg tcttattgag cactctcttc ttgatcaacc cgcccggaat cagcgctttt
    36601 gggtcgagcc tcgtggatta ctggggctca tggagaagtg agaaccccgc gcccctgaaa
    36661 gggattgcgg ctgcctgggg atcgagcacg ctgctcgtgc tggtgctgtc tatttggagc
    36721 ctgatacagg gcttcgttcg cggcgaagcc cgtgccaaat ggctgggaat atgggctggt
    36781 gcagccctgg cactgatatt gatgaaccct gcaagacaat tattcgattt ttcctgggca
    36841 actgtgccat tacttgccct ggcagcgatg aaactagtgg agttgttccc atctttttca
    36901 tctgataacc gcattgtttt catcgcggaa acagttttgg tggtcgcact ggtggttttt
    36961 tcgtttatga acgcggtcaa cctggtcaat aaccctttgc aaagcccgga ggaataccgc
    37021 aaccgagcga ttggcgcggt gctgcccttg ttcctgttac tgggcatgac tgtgctgctc
    37081 gcctggggct ggtctggggc atccacccgg gagggtttca ttgcgggcgt gctgttgctg
    37141 gctggctttt tcaccctggc aaattccatt aaagccgcct cattgggcag ccgaccagaa
    37201 attgaaataa gaagggacct ggcggctgtt gtgggctcag aagaactgct gcgcacaatc
    37261 caagacatct cgcgggcgag aacagggatc gaaaaccgca ttgatgtctt gatagttgac
    37321 ttgcagagtc cctcgcttga atgggcattg cgcagttttg aaaaagccga aaccgcggct
    37381 gtctttgacc cgaccgcatc accctctctc gtgattacag acgttaacca acaaatcacc
    37441 gctggggcgt cttatcgcgg tcaaaaaatt atttggcagg aagagcctga catgaagaat
    37501 atgacgatca tagactggtt gaaatggtca tttttccgta tcgcacctct tgagaataga
    37561 gaaatcatac tctgggcgcg caacgatctt ttcccgggag ggaacatacc atgagtacaa
    37621 ggggtgctgc catgcgcaaa cagcagaaac ctttggtcat cgccatcgat ggacccgccg
    37681 cttcgggcaa atccacgctg gcagaaaaac tcgcccagcg acttggattt ttttattttg
    37741 ataccggtgt tatgtatcgc gcggcaacgc tcgcagcgat gaaggacctg ggcggtgtgg
    37801 aggatgagaa agccgtcacc aggctgacag cggcnnnnnn nnnnnnnnnn nnnnnnnnnn
    37861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37921 nnnnnnnnnn nnnntcgccg gaggaaagag ccaggcggcg actgaaagag atgcagctgc
    37981 ggggtgaaac ccattctttt gaagaggtgc tgcaatccat tatcaggcgg gatggcattg
    38041 actcaacccg gcaacacgct ccgcttaaac cagcagttga cgcggtaatc atcgacacag
    38101 atggcaagtc gattgaagaa gtgctcgaag aaacgcttgc catcgtgcaa ccatgaattt
    38161 atactgattg ttgatttcgt tacaacgaga aggaggattc catgtgcgat accattgtgg
    38221 ctttgggtaa ttccactgtg gacggtaatg tcatttttgg caaaaactca gaccggcacc
    38281 ctaatgaagc gcacgaggtg gtgatctttc ccgcggcgca gcacccaacc ggcaccactg
    38341 tcaaatgcac atacattgaa atcccacagg ttcccgcaac acaccaggtg ctgttggcaa
    38401 agcctttctg gatctggggc gcggagatgg gcagcaatga acatggtgta actatcggta
    38461 acgaggcggt ttttacgcgc accccctacg gcagagaacc cgggctcata ggaatggatt
    38521 tgttacggct ggctttggaa cgcacggata gtgctgaagc cgcaatgatg ctgatcattc
    38581 atctattgga agaatacggg cagggaggca actgcggttt tggacacaaa atgtattatc
    38641 acaacagttt tctgatttgc gacccaaaag aagcctgggt gcttgaaact tcgggtaaag
    38701 aatgggcggc agaaaaggtt agagatgtgc gcagtatttc aaacgccatc accattggcg
    38761 aaaaatggga tctatgttcg gcgaacctgg tcagtagcgc gattaagagt ggttggtgcc
    38821 gttccgcagc ggagtttcat tttgccagat gttattctga accaatctat accacgttcg
    38881 cagccgggcg cagccggcaa gcctgcacgg ctgacagcct gagggcacag aaaggaaaca
    38941 tcaccgcaac aagtatgatg cgggttctac gcgcgcacac gcgggaaata aaacccacct
    39001 ggtcaccaga ccgcgggatt accggagcgg atgtgtgcat gcacgcgggg tggggaccga
    39061 tacgagaaaa ccagaccgcg ggctccatgg tgtctgtgct gcgcagcgga ggtcgttctg
    39121 aacattgggt gaccgccaca gccgtgccat gcacgtcgct gttcaaacca gtatggctgg
    39181 acgcgggagt gccctctact ggaaatgccc ctactggcga atatgatcac gcggcgcttt
    39241 actggcgtca cgaagcgctg cagcgggaaa tcgccaggga ttaccaggcg cgcatcgccg
    39301 tgatcaaaga cgcacaagcg acctttgaaa atgaattaat ggcggaggtt gccagagcgg
    39361 ataatgccac agcgcagcag cggcggctga tttcagaaaa tgcttttcga cgggcggatg
    39421 aaattgaaga agactggtat gcacaaatca agggcatgaa aattacccgg cagaacacct
    39481 tgctttaccg atctgcctgg aataaattca atgctcaggc gaaaatgaaa taacgttgta
    39541 tcataatagt agatgatcag aattcacctc ttcaacgcgg ggccgttttt cgatacgcct
    39601 taccagttgg taggctggat tggttggttt gtcatggcgg cgttgattct atgggttgcg
    39661 cggaaagacc tgacccgaca gcgcacgagc cttatttgga agtacttttt cgccctgggg
    39721 ataatggcga tcgttagtac tttgtttttt ggttttgaat taccacctta cagcaaggtt
    39781 ccgttaccga atatcccggg agagatacaa ccgcccgccg tgatggtgct gatgatggtg
    39841 cccatcatgc tggcagcggg catattgggt acctggccgg cagccgtttt aggttttatc
    39901 gcgggaattt ttagcgcgtt gtggaatacc cactctgtct ttacacccct ggaaatttct
    39961 gttctggctg tggtaatcag cctcgctttg cgccagaatt atcgcacgcg gttcttcgct
    40021 tttttacgca gaccgattgg ggctgccctg cttgcggctt tcttttccat cccgctgttc
    40081 ctggtcagca ctttctttgg cacaaatggc aacctggcgg caaaactcga ttacgccttc
    40141 actcgctcgt gggtgctgat tggcgtttct acgatacagt tgctcatcgc cgcgcttatt
    40201 tgcgaggttt ttcgcgcgcc caattcaaga tattgggtta agttcggctc gtttgaacct
    40261 tcaccagtag agacggggtt acagagccgc attctttaca cagccctgcc gctggtttgg
    40321 atactgctga cgacgctggt catcgcggat tggtttgtcg ctggccgggc ggcgcgtgat
    40381 attctggtga aacaacttca agatacttcc aaaatcgctg tggagaatat cccgccaatt
    40441 ttggagaccg gtcaaagcct gacggcggcg ctggctaccc agaaactgcc cttaggtgat
    40501 acacaacagc tcagagattt attgcagagt aaagtacggg aattcccatt cttttcccaa
    40561 atatacattt ttaacctgac gggttctccg ctgactggtt acccagatga cgaagttggc
    40621 gaatttcgtt tatcctctga agaggagctg ggaatccaac tggcgttgaa cggggtgcaa
    40681 atgcagaact atgtgatcca cccctatccc ggtgcggaat ccgcggtaat ctcgtatatc
    40741 gctgcaattc ctgatgaata cggtttagcc caaggcgtgg ttattacccg tacagacctg
    40801 acgatgaacc tgttttcaca gccagcgctg attgccttgc agaacctggt tgaagaaggt
    40861 ggtcagggcg tgatcataga cgaaacaaat cgcgttctgt accacaccaa ccccaagttg
    40921 attatgacca cctataccgg gcagattccc gctgtttcgg ggtattttga agaccccggg
    40981 ctcacgggaa cccgctatat gaactatgcg tctgtggatg acttaaatgg ctggaaagtc
    41041 attctctcgg tgcccgcaaa tcattctcag caattggcgc tgcgaatagc tattccgttg
    41101 ctggtcatct ccctcattat ttctgttttg gcgtatttat tattgcgtta cctgataaaa
    41161 acactcactc agtccctcgt tcttttagcc aataaagcgg atgaaatttc caaaggcgag
    41221 ctggataacc gcgtcgaggt aaagggagtg gatgaaattg gccggttgag ctccgctttt
    41281 gagcagatgc gcctcagcct gaaattgcgc ttggaggagc tggacgcgct gctgaaggtt
    41341 tctcaaggaa tcgcttctga cctctcattg gaatcaactt cggcgcatct attgagtgcc
    41401 ttgcgatcgt atggggcgga tgccgccagt ttggtgctta cccggggaac gaaggagggc
    41461 tgggacgaag ggttggaagc ctatcgatcg ggtgaacagg ccgaggaata cgcctacctc
    41521 gatcacttat tgctggaaca tttacaggaa gagtccctat tgatcatccc atccagagcc
    41581 agaatccggc gcatggcttt acccaaaaca gcacccattc cttctgcttt agctgccatc
    41641 gccttgaaca aaagtggtga aaaccctgga tttatttggg ttgtttacac ccaatcgcat
    41701 cgtttcctgg aacctgagat tcgttttctg gataccctgg cggggcaggc actgttagct
    41761 gtatctaact cgctgcttta ccagaaagcg gaagtaggca aaaaacggct ggagtctgtg
    41821 ttatcttcaa cccctgaacc ggtgctggta gccggtgaag acggacggct aatgatggta
    41881 aaccaggcag cggaagaaat gaccctgtta atacaattga acgctcaagg tactgaaagt
    41941 cgcggcgaga tcatctccag gctgttaaaa gaattcatca ccggcaccaa tcccacggag
    42001 agccgggcag aggaaatgga acttgaagac catcatattt acctggtttc agtatcaccg
    42061 gtagcggtgg aaagcaagca cctgggaaag gtctgcgttt tgagagatgt caccgattac
    42121 aaacaattgg agaagatgaa atcagagtat gtttctaccg tcagccatga tttgaaagcc
    42181 ccgttagtat tgattcgggg ttatgcatcg atgctgccga tggtaggcga tttaaatgaa
    42241 caacaacgag attactcaca aaaaatcctg gacggcatcg atgatatctc caggatggcc
    42301 gacaaccttc tggacatgcg caggatagat tctggtatcc agctgcaggt ggaaaaggtg
    42361 tcgccctcag acctgcttga tgaagtgatt gaagagatgc agccgcagat caaacaccgc
    42421 aagatacaga ttcttcgtga actgacgctt gcgcaaaacc tgaccattga agccgacagg
    42481 gtactcttgc agcgcgcctt acataacctg ttagataacg cggtgaagtt ttcttcatta
    42541 ggaagccagg tgaacctgcg cttgcaggta aatgaaaata gcgtggtttt tgagattcag
    42601 gatcacggct cggggattgc gccgttggat cagccccatg tatttgaaag tatcagaagg
    42661 aatacagatg ggagcccgaa gaaggaatgg ggagtgggac tgtcgatcgt caggtccatc
    42721 gcggagcgcc accagggaag ggtatggctt gaaagtcaat tggggaaagg cagcacattc
    42781 tacctggaaa tccctttaca gcatcaggcg gcagaaacaa ataaatagta aagaaatctg
    42841 aaatttgtgg taaaatacca gattgcatgt atacaatggc ggaatgttgc ctgttcgtgg
    42901 tcgtaggcaa aattctgatt ggacaatttc gtttttttaa ctgctgcagg tctttctcat
    42961 tcaggaggca ttagtggaaa caaaaagttt agtcgcgctc cgtatcaatc tcgcatcccc
    43021 tgaaacaatt cgaagctggt cttatggtga ggtgctcaaa cctgaaacga tcaattatcg
    43081 ccggttaaga ccagagaaag atggtctttt ttgtgaggcg atctttggac cttcgcgaga
    43141 ttatcagtgt tattgcggta agtacaaaaa tccacgttac aaaggtattg tttgtgataa
    43201 atgcggcgtt gaagttactc gttcttcagt tcgccgtgaa cgcatggggc atattgacct
    43261 ggcgacaccg gtcgctcata tctggtatac ccgcaggatc ccctcttacc tgggtctgct
    43321 attggatatt tcccggcgta atttggaccg cgtgctttat ttcgcacagt acattgtcac
    43381 atttgttgat gatgatgcca ggcagaaggc gctgcgcagg ctgatggatg aaatcaatga
    43441 ttccgagctg gaacaggcta accgcatcaa cgcccagatc ctggaagtga agttgggtcg
    43501 agacaagaag gtggctgaat tacagagccg cttgaaagaa attgaatcga aagccgaaga
    43561 acaaattact tcaaagctcg aaccggtcat tcaggagggg caatctcttg aacgacagct
    43621 taccgagctt tcaggaaaag ccgccaaaaa gaagatcttc tttgagactg cagacgtcat
    43681 gatcgcggag aaaggcgaga ttattgctcc ttcacacatc gccagtgtgc agaaagcggt
    43741 gaaggaacgc ctggaagaga tagaggacga atacaaaaaa caattggaca tggaccgcga
    43801 acagcttaaa atggccattg aacaggtcaa agcagaagcc gaagtttcca tggatgaact
    43861 gcgcaaccag ctggaggatc aatcaactga atctcacgat ctttctggca gaatgcgcga
    43921 tgaactggaa gagcttcgcc ctttcacctt cctcactgaa agccgctacc gcgaactgaa
    43981 atcccgatgg ggacaggtat tcagggctga tatgggcgct gaagcgtttt atgatatcct
    44041 gcgccgcatc gacctggata agctgtcaga agatctgtgg actgaagttc gcaccacgaa
    44101 gagcaaacaa aaacgaaaaa aggctaccac tcgactcaaa gtaatcgagt ccttccgccg
    44161 ctcaggtaac cgccctgagt ggatgatctt aactgtgctc ccggtcattc cgcctgactt
    44221 gcgcccgatg gtgccgctcg atggtgggcg gtttgccact tctgatatga atgacctgta
    44281 tcggcgggtg atcaaccgta ataaccgcct gaagaggttg ttagaacttg gcgctccaga
    44341 tgtcattgtg cgtaatgaaa aacgtatgct ccaggaagcg gtcgactcgc tcattgacaa
    44401 ctctcagcgc ggcaaggcgc tctcccgccg cggacggcgt gaattgaagt ctctcagtga
    44461 tatgctcaag ggcaagaaag ggcgtttccg ccgcaacctg cttggcaagc gcgtggatta
    44521 ttctggccgc tcggtgattg ttgttggacc aaaactgaag ctataccagt gcggtctgcc
    44581 aaaaacgatg gcgcttgagc tgttcagacc cttcgtagta gcacgccttg tgcagcactc
    44641 ctacgcggcg aacgtgaaat ccgcccggcg tttgatcgaa cgcaacagac ctgaggtgta
    44701 tgaagtgctg gaagaggtca tcaaagaaag acccgtgatg ttgaaccgcg cgccaacgct
    44761 gcaccgcctg ggcattcagg ctttcgaacc tattctaatc gaaggcagcg ccatacaact
    44821 tcacccgttg gtgacaacgg ctttcaacgc cgactttgac ggtgaccaga tggctgtgca
    44881 cgtaccgctt tcagacaaag cggttcaaga agcgcggaac ttgatgcttt cttcaaagaa
    44941 cttgctcaaa ccagccgatg gcgaaccgat catcagcccg gcgaaggaca tggtgctggg
    45001 tatttactac cttaccgttc aggataaccg caaacacaaa ggcgatggcc gggtcttcgc
    45061 agatatggat gaagttgagc tggcctacaa ccttggtcag gtagaccttc atgccaatat
    45121 aaagattcgc atgaacacct ggttcaacgc tgaaaacaaa cgcatgccca aagctgaaac
    45181 cagaatgata gataccacgg ttgggcgtgt gctcttcaac cggattctgc ctgaagaggt
    45241 tcaattcatc aatttcgtgc ttgaaaaatc tggggtaaaa gatctcatcg ccgatgttta
    45301 tgacctgtgc ggagaatcgg tcacaactga agtggctgac gctattaaag atattggctt
    45361 tgagtacgct atgaaatccg gcacaacgat cgcggtcgcg gatattacca ttcctgaagc
    45421 aaagtatgaa attattggct cagcccagca ggaaattgac cgcatcaacc gtgactatcg
    45481 ccgtggtcta ttgaaagaag aagagcgtga caaactggtc atcgacatct ggaaaaaaac
    45541 gactgaagat gtaggagcgg ccgtgcgcgc ggggatggat cccaacggaa atctttccgt
    45601 aatggcctct tctggcgcca ccaaaggcgg ttttggtcct atttcgcagc tggctggcat
    45661 gcgcggcctg atggcggacc ccgccgggcg aattatccgc ctgccgattc aatctaattt
    45721 ccgtgaagga ttgacggcgc ttgaatactt catttccact cacggcgcgc gtaaagggtt
    45781 ggcagatacc gccttgcgca cggctgacgc tggttatttg acccgccgcc tggtggatat
    45841 tgctcaggat atcattatta acgaggaaga ctgcggaact gaaagtggca tctggatccg
    45901 cggcgcagat gatgtggctg gacaaaaatt aaaagaaaga ttgtacggtc gaacggtcgc
    45961 gggtcgaatt gtgaacccca agacgggcga aatcatcgcg gaacgagggg acattctgga
    46021 ccatgacctg atccgcaagg ttaccgacgc cggcgtagaa gatgtgtatg tgcgttcccc
    46081 tctgacctgc gagctgatcc acggtatttg tgccaagtgc tatggcatgg acctggggcg
    46141 cggttctctg gtgacactgg gctctgctgt gggcacagtt gccgcccaat ccattggaga
    46201 gcctggcacg cagttgacgc tgcgcacctt ccacctgggc ggtgtggctt cggctggaga
    46261 catcactact ggtctgccgc gcgtggaaga acttttcgag gcgcgccggg ccccgaaagg
    46321 tgaagcggtg atcactgcaa tttcagggat tgctaaagtg ttgcaggcag atcgcgttgg
    46381 tgaccagaag ctcgtgcgcg tggaacacag cgaactggtg tcggatgagt atgaaattcc
    46441 caccgattgg gagatactgg cgaacgatgc cagcgagatt gtcaccggtg atccggtcgc
    46501 cagaatgggc gatgccttga tcaacgccca acatgacgga agggtgcgcg ttgaaggtcg
    46561 taaggtgatt gtttcatatg agcagaaaga gagcgaagat tacgagattc ccggaacttc
    46621 gcgcatgatt atccgcgacg gtgaccaggt tgaagcggga caggcgttga cggaagggtc
    46681 catcaatcct cataccatcc tcaagatcaa ggggcgcgag gcttcacaaa tgtacctgtt
    46741 gaacgagatt caaaaggtat atcgagcgca agggcaaaac atcaatgaca agcactttga
    46801 agtgatcatt cgtaagatga ttggaaaggt gcaggtcatc aggcctggcg acaccaacta
    46861 cctaccgatg gatgtggtgg accgattgga gataaagcgc gtcaatgaag aaatgattgc
    46921 ccagggtaaa cagcccgccc gttatgccga gattttgctg ggagtgacca aagcgtcgct
    46981 gagcactgat tcgttccttt cagccgcttc gttccagcat accatcaaag tgcttgctgg
    47041 agcggctatc gcctcttccg aagacccgct gtttggcttg aaagaaaatg tcatcattgg
    47101 caagctcatc cccgctggca ccggttttgt ctacggtcgg tttatcccag cggttgaagg
    47161 cgaaaccgca gaggtcgagg ttgctgctga acctgaaacc gaaacgaaaa ttgaatagac
    47221 ttgaataatc atttaccaag aacactccgg attaaaatac atccccggag tgttttttta
    47281 atgaaagcct gatagggctc tttagccgca atctttttat tgtaaactta tcttatccaa
    47341 taaaaaaccg ccggagatta gattatggat atgggttctg ttcgcaagat tgatattgat
    47401 cacgaaatgc agcaatcata cctggattac gccatgagcg ttatcgtatc tcgcgcattg
    47461 cctgatgcca gggatggatt gaaacctgtg cagcggcgat tgctgtatgc catgtacgac
    47521 atgggcgtca gggctgactc tgcatataaa aaatcggcgc gtattgttgg tgaagtattg
    47581 gggaaatacc acccgcatgg agaccaggcg gtttatgagg cgatggcccg tctggcgcag
    47641 gatttttcaa tgcgctgcct gctcgtagat ggacagggaa atttcggcag tgtggatgga
    47701 gaccctcctg cagccatgcg ttacaccgag gcaaggctga catcgatcgc gatggaattg
    47761 ctcagtcaga tcgatcgaga tacggttgat tttgtgcgca actttgatga ctctctggat
    47821 gaaccactgg tgctgccgtc tgccatcccg aacatgctgg tgaatggcgc ttcaggcata
    47881 gcagtgggta tggctaccag catacctcca cacaacctgg ttgaagtgat tgacggcctg
    47941 gctttcttgc tggataactg gcaaaagatg gaagatatta ccgttaccga cctgatgaaa
    48001 ttcatcaaag gacctgattt tcccacgggc ggcatcattt tgcaggagca tgaacaggac
    48061 gagatccttg cggcttacgc cacgggcaag ggcagaatca cgctgcgagg caggttgaac
    48121 ctggaggaaa tgtcccgcgg aaagagccgc attatcatca gcgaactgcc ctatatgacc
    48181 aacaaatctg ccttgattga gaggatcgcg gagctcaccc gtgaagggtt gctggaaggc
    48241 atttctgatc tgcgtgatga atcagaccgg cagggaatgc ggattgtcat cgaattaaac
    48301 aaggcggcgg atgctgaagc cgtgctgcga gagttgtata agcgcactcc gctgcaggtg
    48361 accttcagca tcaacctgct tgccctggtc aataacgagc ccaggctctt acctttaaaa
    48421 caagccttgc gggtgtatct tgagcatcgt attgaggtgg ttaaacggcg cagtgaattt
    48481 gacctcagga aggcacaaga aaggattcat atcctggaag gactgaggat cgcgttgcag
    48541 aacctggatg aagtgatcgc cattataaaa aaatccactg atacggatga cgcgcgcgag
    48601 cgtttgatta aaaaatacaa acttaccttg atccaggcga acgcgatcct ggacatgccc
    48661 ttgaaacgcc tggctcagct ggaaagaaaa aaaatcgaac aggaataccg cgagctggtg
    48721 cagttgatca aagagctact ggcgctgctt cgttcaccgg cgaagcaacg acaggtggtg
    48781 ggtgatgaac tgaaagagat ccgcacgaag tacgctgacc ggcgaaaaac acagatcgtt
    48841 aacctgaaga aaggcaaggc tgctgcagac cttctgacgg tgaaggatgt taccccggtt
    48901 gaatccgttt gggtggtaat ggacgaacag gggagaatcg gaaaaataaa cgcggannnn
    48961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgc attacctggt cgtcacccgt
    49081 tcaggaatgg caattcgctt tgaaggcagt gaggtgagac cgatgggact gcttgccgcg
    49141 ggagtcaacg ctatcaagct gagcgctggt gatgaggtgg cgggaatgat agaaattgcc
    49201 cgcaaaggag acgtggtact ggtagccagt aacggggcag gttggcgcgt tgagccagat
    49261 ggtttcccgc ttcagggtcg ttatgggcag ggagtgattg gctgccgtat ggctgatggg
    49321 gtggggttga ttggtgtggt gtttggtaaa aagaacttcc aatataccct tcacctgaag
    49381 aaagccgcag ccaaaacgtg cagagtggat gatatcggct acaccaaacg cgcgggtaaa
    49441 agtgagcaga tcctgcagct ggcttctgga gaccgggtag aaagaattgt acggatgatg
    49501 gattctgcgg aatgagcgtt cataccgcgt attactcaat tgagtagtag cagtaatggt
    49561 ataacaccct gccgtctttt aaaccgacgg tgtcttttcc attattaacg atgcctcttc
    49621 gagagtggca ggtccatgta tagtggatga cgttgtcttt tacctctttg gaggtgactc
    49681 tgaaatcacc atcattcaac tggttggtaa gcatctggcc aaagaaaccc cggatattgg
    49741 cattcccctg gatggtctgc tggggggtaa ccaacacagc gttgggggcg taaatttcca
    49801 acatggggtc atattgcatg gcgtttaacg cgtcgaagag acgagtaacc gggtcggagg
    49861 acggcggtga aacgctcccc tgcgccggcc aggaatacct ggcaatctcg tcccatacag
    49921 gcacgagatg cacccgggaa taatcccacg agtagaaatt cgctgccgtc aaccccaaag
    49981 accgggcggt atcaagaaat agttttacct ccgacggaga ggggtaccat ccgctttcac
    50041 catacgccgg gcccgtgggc acaatagggc ggaagggaga tatggcttta aattcattaa
    50101 cgctgcgttt gagatcataa tccgggttgt gtgcttgcat ccaataaacc tgcggcatgt
    50161 tcaaatcgca tttttcaagg aaggcggaat acgggaaaga gcggtgatag gttggccatc
    50221 taaaagaact cagcgctacc ggcgttgaag gaataccttt acgcaactgc gtcatgaaaa
    50281 ttttcgcaac agcttcgcgc ccggcttgct tatattccac ctccgcatca atcacataac
    50341 catctaaggc gtattttcgg gtctgcgaga cggcaatatt ggcttcacca actgggtcat
    50401 atccgtaaac ataatgccag ccccaaacct ggatgccttt ggcgcgcaac gcatcgacca
    50461 ccggcgggat caagtctcta ccgctgctat cgacgttgta tgggcgggta tcattcgcta
    50521 tcttaatgag gacgtgtgaa aacccagaag aggctgccac ccgggcgata gctgatggat
    50581 tgccggcttc gcagctgggg attttccata tcatcatacc tttcccggtc aaggtcatcg
    50641 atctctcctc tgatcagcca ggtgatgaac ctggctccag gttaccagca agcccgattt
    50701 gcgtggcgat ggcgcgcatg gcgatgatca cattctcgca atgctcccac aacgggacac
    50761 caaattcttc agcgccgcga gcaatctctt cgcggttggc tcctgcggcg aaagccttat
    50821 ccttccactt cttcttgact gaactggctt caagatcata aagtgagcga gaagggcgca
    50881 ccagggcaac cgcagtgatc aggccagtaa tttcatcgca tgcgtataag cccttcgcta
    50941 gcaaactggt gcgagggatg ttgaggtttt ggccatgggc gagaattgct tggattacct
    51001 cttctgctac accgtgttga cgcagtatcg gcgccccctc caacggatgt tgctcagcgg
    51061 atgggtgaat ttcccaatca aaatcgtgca gcaagccagt tacagaccag gtttctaccg
    51121 gctgccccaa tttttcggcg taaaaacaca tagccgcttc caccgccagc atgtgatgga
    51181 cgaggttttc attttttaca tattgtctga cgagttttaa cgcttcttca cgattcatat
    51241 tttcagtggt tatcggactg ctcagcaaaa atgggctcgg gttttcccag gacgggtaaa
    51301 ggtttggaaa cccatacctg ccataacgcg accagcgtgg caggcacctc gcgaatacgc
    51361 caaaaacgct ccgcgtggtc atgataatca cgcaggtcag cgattacacc atttactttc
    51421 atgcccatcc cgctgcaggt aaaaatggct cgagggagat ggaacttttg ggttacaacc
    51481 agaatatcat ccagaccgaa gatgtctctg gcgcgataac atgtgtcgta ggtgcgccgg
    51541 ccggcaaaat ccagcacaat ggcttcttcc ggcactccct ggctgagcgc gtagtcgcgc
    51601 attgaagccg gctcattgta atcgacaaag cgattatcgc cactcatcaa taattttttc
    51661 acctttcccg cctggtaaag ctgcacggca gcggtcaccc ggtctttaag cactggggag
    51721 ggagtaccgt ccttttgcaa accggcccca aacacaacag ccaccggcgc tgagggggcg
    51781 tcagctacgc tgtatattct gccggcagca gaaagtgagg ttaccacctt cggaagcccg
    51841 aagagcacca gtaacaggag gattgaaaaa agcaccagtt ttaaaagcat ttttattatc
    51901 cttgaaaaca tagcaagaat tgtaccagta aagcagccag ttctaagacg ggccgatcga
    51961 tcctgatgaa aggtctaaat tcgaaatgtc cactgcgcgg atccgtattt tgcgcttacc
    52021 aattcagcgg ccattttaag ctcttgttcg ctgggctggc tggaggagaa atgtatattg
    52081 cccgccttac tgaaaccagt tataacggcc tccgcgacag tctccatgct cggcgcggtt
    52141 cccattaccg cttccagtgt ggaagcgcgg gcgatcagcc tgttggcggc tgtttgacgc
    52201 gcttgttcgg aagggaattg gagtacccgg gtgatgcggg cgaggtcacc tttgattggt
    52261 attgaaccat gttgcagaat accgccgtac tttcttgact gagcagaacc gatcaatttt
    52321 tttccatcaa ccgtgatctc ataattggaa ggtgtttcaa agcaaaccgg gtttttctta
    52381 ttgtccggcg gttcattggt gtaggcttta tctccgaagg ctgtcacatt catgcgggcc
    52441 aaaccggtca gtaaagccct tgaaattttg cagtaacttt ccagcaggcc acccgccagg
    52501 aagggctcat catcgctgcc tgccacagcg taagtgagct catccacatg caatatcgcc
    52561 cggccgccag ttggtctgcg caccagcccc cattgttctt cttctaaggc agcaagatca
    52621 acatcaaggt gaggttgagc aaatcccagt gatagagtcg ctggttgcca agcatacatc
    52681 ctgagggttg gaggtgagtt tttttgcgct gtggaaaata agatggcttc atccaagccc
    52741 atattccagg ctccggatgc gggaggagaa aggatgagcc gccattctct taacatatct
    52801 gggtaatttt actacgagta atcagtgcgg cggtgagcgt aatctgtcta aatagatatg
    52861 aattatcttt atttacttga caaaaatgat aggaacggct aatattaaac cgctttgaaa
    52921 aacaatatgg aaaatctcgt cttaatggtg ggtgagcgac tgggagttcc gcaaatcaga
    52981 gagaacctcc gaagtatgaa tttttcactt gccatcaccc tgaagggtga tagatagatt
    53041 ccacatgcaa caaggaggtg gttgacaccc cacgtttacc ttaaagcgac aacatgaatg
    53101 tctactatgt tcaaaactta aaaaagagga gaaaaaatga caaaaaagtt gttccccctg
    53161 tttagcgtac tgcttgtggc agtcatgctg ttggccgcct gcggaccgaa agaagcaggc
    53221 cctgtaacca tcaacctctg gacgaaagaa ggtgaggcag atggtggttt gcagtatgtg
    53281 caggcgcttg ccgatgccta tactgctgcg caccccaatg tgacctttgt ggtgacgaac
    53341 aaggaagttg agactctacg tgaagacttc cagaccgcca gcctggcgaa tgcagcccct
    53401 gaactgcttt ggactgtatc tgaccacctc ggccccttta ccaccgctga ccttattcag
    53461 ccggttgata aactggtaga cctgggcaag tacgtgggca gcgcaacaga cgctgtcaag
    53521 acccccgatg gcaagacctg gggcgttccc attgccaatg gcaaccacct gatgctgctc
    53581 ttcaataaat ccttgatgcc agttgcccct gccaacaccg atgaactcct caagatggga
    53641 gcggattttg ttgcagccaa ccctgggaaa tacgccctgg ccttcaatga gactgaagca
    53701 ttctggtttg tgccctggtt gggcggcttt aagggcaaag tgttcgctgc tgatggcgta
    53761 actccaaccc tgaatacccc tgaaatgatt gagacgttta aattcctggc tgaactcaaa
    53821 gccgccaata ttatgcctgc cgagagcaac tacgatgtga tggacgcggc cttcaaagaa
    53881 ggcacggccg ccatgatcat caatggtgac tggtcgctgg gtggatacaa ggaagccctg
    53941 ggtgaaaacc tgggtgttgc cccaatcccg aaggttgttg ccactgatac ctatcctgct
    54001 ccttacacct ccggtgtgtt cttcatggtg ccgaaaggcc tcgagggcga gaaactggac
    54061 gcggttgttg acttcatgaa attcgcaacc aacaaagaga accaggttga aatggtgaag
    54121 aaattgaccc gcctgcctgc gctcactgcc gctctttcag acaatgtgat tactgaagac
    54181 cccattctgg ctggctctgc cgctcagatg agctacggta cacccatgcc caccaacgtt
    54241 gaaatgcgct gcgtttggga tgcgattaaa cctgaactgc tgcctaccta tgcgggcacc
    54301 acttccccag aaaccgcagc cgccaacgct caaaaagcgg ctgaaacctg tatcgctggc
    54361 ttagagtaat ccacaataaa agagaaggtg ataaccttct cttttatcaa taacacacgc
    54421 cgggctggat attcagcccg gcatatccta taaatatagt ttggataagg aggaaatctc
    54481 atgatcacat cagaggtgat cctgaaaagc ctcggagaag tgggtacgac ggtactcctg
    54541 attcttattg gaacagtcct ggtagaagcg ttgctttacc tgattctaat gaaatggttg
    54601 aaatataaat acgcgctacc ttttatgtta ttggctccag ccgcaatcgg ccttatcgca
    54661 ctggtgattt atccgctgat ttgggaactt cgactttctt tcacaaatat gtcattaaag
    54721 gcttttcgcg acccggcatt tattggcatc aaaaactatg ttcgcgtttt caccgaacca
    54781 gtattaaaac aaaaaatgtt tttccctcta ttgggacaga caatcttgtg gaccgtcatt
    54841 aatatcacct tccacgtggg gggcgggctg ggtttggcga tgctcctcaa tcggaagatg
    54901 agaggcaaag gtatctatcg ggcgatcatt atcttgcctt gggctgtgcc gcaggtcatc
    54961 gcggtgctgg cctggcgagg agagttcaac ttcgcctatg gatacttcaa tatcatctta
    55021 acgaaactgg gtcttccagg agttcaatgg ttggcagacc cggtggctaa ctttgctgcc
    55081 atgtgcatga cgaatatctg gctgggaatt ccctttatga tggtcaccct gctgggtggt
    55141 ttgcaaagta tttcctctga attttacgaa gcggctgaaa tggatggcgc aaacgcctgg
    55201 cagcaattca gaaatatcac tcttccactc attcaacctg tattaacacc agcgatatta
    55261 ttaggaacat tctggacatt caacaacttt aatgttcctt tcttcatcaa tcagaacgag
    55321 ttggaaagtt ctgatattct ggtaaccgga ctcttcaggg ctgcattcca gtacaaccgc
    55381 tacggttttt ctgccgcatt cgctttcgta atcttcgcgc tgctcttgtt gttcaccatt
    55441 ttctatatgc gttacaccag gtctttaaaa ggagcgtatg aatcatgagc gccaagcata
    55501 caactcccaa aaagtcattc ttccgcagcg ggcgtgagga ctcacccttc aagagaattc
    55561 tcatccatct tgtgttgatc atttcatgcg tcatcgcgat tttccccatt ttgagaatcc
    55621 tggcagtatc tctgcgcccg ggcaaccgcc tgatgtccac cgacctggcc atcattccgc
    55681 ctgacgctac gcttgaatct taccggatca tcctgtttga aaagcctttc ttgagttggg
    55741 tttggaacag cctggcgatt accatctcaa ctgccttgat cggtgtcatc ctggcggcta
    55801 ctgctgctta cgccttttcg cgttggaagt tccccgggcg ttcggtaggg ctggtctttt
    55861 tactggcaac ccagatgata cccgcctcga tgctgatgat tccaatctat atccttgcga
    55921 tcaagctggg catggtgggc acgtatcgcg gtttggtcat cgcatactgt gtcacctcaa
    55981 tcccattcag cgtctggatt cttaaagggt attacgatac gatcccggtt gatcttgaag
    56041 aagccgccag gatcgatggt tgctcacagt tacaggcttt cttgcgcgta ttgctaccat
    56101 tatcaacccc ggcgctggcc attaccttcc tgttcaattt tatggccgct tggaacgaat
    56161 acctgctggc acgcgttatg ctgggatctc aagagagttt gctaacctgg ccgctgggat
    56221 tgcaacgctt gcagggtcaa tttcaaaccc agtgggggca gttctccgcg ggctctatcc
    56281 tggtgatggt cccggtgatg gtgctgttcc tgtattcgtc aaaatacctt atttccggcc
    56341 tgaccctggg cggtgttaaa ggttaataga accaaatgag cgcataaaac cccggattct
    56401 gctgggaact ccgggtattt tggattaatt catgtaagag gtgtgtcgat gaaaaagacc
    56461 ttcaaatatc tgatctttat ccttgtgctg ggtttaatct tgagctcctg cacctctccc
    56521 actggagata cccgtaaagg cattggcagg aaggatgtgc tctatctcaa tcttacctgg
    56581 catcaacatc aacccttata ttacaaagat gaccagggtg tttacacgcg accatgggtg
    56641 agggtgcacg cgaccaaaga ttattacgat atggctgcca tgatcaagcc ctacccgaat
    56701 gttcgggtaa ccattaattt aaccccggtt ctgatgaaac agctggatga ttttacacag
    56761 aacggcgcta aggattatta ttgggtgctg gcggaaaaac ccgcagcgga gttgaccgac
    56821 gcagacaaaa cctttttact ttcccggttc tttgatgcaa attgggataa tatcatttac
    56881 cgattcccgc gttacaagga gttactcaac aaacgtggcg gtacaagcgc agaagccatc
    56941 gctagcgcag tatcacaatt tagcgagcag gattttcgcg acctgcaagt atggtttaac
    57001 ctggcgtgga ttgaccctga attcctggca cagagccctt tgaaagaact ggtggaaaaa
    57061 gaaaggggat tcagtgaagc ggataaagca gttcttttca atgaggtgcg gaaaatcatg
    57121 gagatggtca tcccggtgca caaggaactg caggacagcg ggcagatcca ggtgatcacc
    57181 accccttacg cgcacccgat attgccgttg atctacaatt caaacatcgc ctctgttggc
    57241 aacccagcgg ctgaactccc tgcaagattc tcgtggccga acgatgccat tgttcagctg
    57301 caaaaaagtg ttgatatcta tcagcaacat ttcggtcggg ctcctattgg cttgtggccg
    57361 ggagagggag cggtatcaga agatatcatc ccgctggtgg cggacgcagg gtatcaatgg
    57421 atgcaaacag gtgaggttgt tctggcgcaa tcactggggt tgggtcaatt tacccgtgat
    57481 tctgctgaaa ctgtgcagga agccgatgaa ttgtaccgcc cttattatgt gaccacaaaa
    57541 gatggacgac gagtggccat tttcttccgc gacctggtcc tttctgataa gctggggttt
    57601 acctactcgc aaacgcccgg cgaacaggcg gcggctgacc tgatgcagcg gctggaaaat
    57661 attcggctac aactgaaaaa acagaaggcg caaggacctc atattgtttc gattgtctta
    57721 gatggcgaga acgcctggga atattatcct aacgatggta aggatttcct gaacgccatg
    57781 taccgaatgt tgaatgagag cgaaaccatt aagatggtga cgccaactga ataccttgaa
    57841 ctgtttccaa aacaacgaga actggacact cttttccctg gtgcctggtt cagccccaat
    57901 tatgatacct ggattggcga agcagaagaa acccaagcct ggaattactt gggcaaaacg
    57961 cgtgaatttt tggccaaata tgacgttggc aaaacgaagc aagcctcccc tgaagccatt
    58021 gccactgcac aagactacat gtacctcgct gaaggttcag actggttctg gtggtatggc
    58081 gctgaccagg actcaggagt ggatgaatac tttgatacag gattccgtgc cttactcgca
    58141 aaagtgtacg agtctttagg agaacccgtc ccagcctttg tcagggttcc aatcatcccc
    58201 agaagacccg agcagcctgt tcaggcattc tcgggaatga tggcggtggt ggtagatggg
    58261 aaaaccgacc agaaagaatg ggcaactgcg gcggcttatc ctgcgaatgg agaagatggg
    58321 ctggaaatgc tgtattacac catggatgct cagaatattt atttccgcct tgattttgac
    58381 aaagaaagag aagaaccgga aagcgtaggg atttacatga gcatcccgag cgccgcagca
    58441 aacgatcctt tcgcgttgac agaagacgct ccggacagcc tgttattagg gatccccgca
    58501 agccatcttt ttgaatgcaa cgcagggctt gtgagcggtt acgaggtatt tgctggtaaa
    58561 tggcaattga acccagaact gaaggcaacc gctgagatgg gggagggact gcttgaactg
    58621 gctataccat taacctcgct gggcgaattg tcggcgggtg atgatatacg cgttgtcatt
    58681 gttgtccagc ccaatagcca ggttttaccc ctttctggac cggctcagat cgtgctgcct
    58741 gacctgggat tatctgacct ggtgcttgaa ataaatgacc ccgaagggga cgaccatgga
    58801 ccgggaacct atacttaccc gaacgatggc gttttctcga aacaaaactt tgatattgaa
    58861 agtttctccg tcgccacaga cgcgaaaaat gtgatcttta cgttcaagtt ctatggccct
    58921 gtgcccaatc catgggggtc ggggatcaac ttatcgctac aaacaatgga tgtgtatatt
    58981 gaccaggacc ccgggaaagg cacgggtaac cgtatgcttc tcccagggcg caacgcttcc
    59041 ttgaccgcca ggaatggctg ggagtacgtc atttgggcgg aaggctggaa ttatggaatt
    59101 tacgcgcctg acccagccac tggtgaacca aaacaacaaa actcatcgta caagatcctc
    59161 gttgaccctg ccaggcagac agttacctta cgtgtgccga aagaagcttt aggcgatggc
    59221 aacccggctg aatggggata cgccgcagta gtgctcagcc aggatggtta cccctccact
    59281 ggtgtttggc gagtaagaga tgtgcagcag accgctgctc agtggaagct tggcggcgcc
    59341 ccggacgata ccaatcacac ccgcattatt gacctggcat ggccagcgga gggcaagggc
    59401 acgcaggaag agtttttaag cacctatatc agttcaaagg cggctatcga aacgctttct
    59461 gagaaggata tgccgcaaat acccatcctg ctggtgaaat agcatggtag aacctgctgg
    59521 gatgaacaac ccagcaggtt cttgcttcac tggtagggat gttccattgc taacaattca
    59581 attctgtgag aaaatgacgc agttttggcg aagacgattc acctgttcta ttgaggatgg
    59641 tatacatgac gattcagact cctgattggc ttaaagacgc ggttttttac cagatttttc
    59701 cggaccgctt tgcaaaaagt gaacgggtca aacgcgaagg gctgaaactg gagccgtggg
    59761 acactccgcc caacccatat ggttttaaag gcggcgatct ttacggtgtc attgaaaaac
    59821 tggattacct ggtcgacctg ggggttaacg ccatctactt taaccccatt aacgcatccg
    59881 cctccaacca tcgttaccat acatatgatt atttcaagat cgacccgctt ttgggtggtg
    59941 atgaagcctt cagggatctg ctgcatgccg cgcacagccg cggaatgcac atcgtaccag
    60001 atggtgtatt taatcatgcc agccggggtt tctggcagtt tcatcatgtg cttgaaaatg
    60061 gagaaggatc gccttatgtc gattggttct atttcgaccc ggagcgatta aaacggcaca
    60121 ggccttgggg ggcttaccct gacgaaagag aacaacaaca attgaacaac ggagtcggtt
    60181 ccttcgatgc catcgggtat acggcttggt ggaacctgcc agcgctaccg aaattcaaca
    60241 cgcaaactca agcggtccga gagttcatct ttgaagtcgc ggagtactgg ctgcgttttg
    60301 gcgcagatgg ctggcggctg gatgtgccgg gggagatcaa tgatgactcc ttctggcagg
    60361 agttccgccg cagggtgaaa gccgtaaacc ctgatgccta tatcgttgga gaattatggc
    60421 ttgaatcgca acgttggtta aaaggcgacc aattcgacgc ggtaatgaat tacctggtca
    60481 gcgccgcact gatgggttgg ctgattggtg ataagatgcc agaatttgcc taccgcatcg
    60541 gtggattaca aagagtgatg cgaccgttta gcgcagccga atttggcgac cgcatcgaat
    60601 acctgttgaa cctgtatgac ccggagatca attacgcgca actcaatctg ctggacagcc
    60661 atgacactcc gcgatttatc actacagcca gcggcgatga aagtgcgtta aggctggctt
    60721 acctgttcct gttcactttc atcggcgcgc cttgtattta ttatggcgac gaggtggggt
    60781 tggacggcgg ccctgaccct gaatgccgga agtcttttcc ctgggatgag tcacgctgga
    60841 atcaagggtt acacgcttat atgaaagacc tgatccattt gcgtaacgcg cacccagcat
    60901 tgcgccgcgg cagttataag agaatttttt cagacaacga agcgcttgct ttcatccgcc
    60961 agtatatggg cgagacgtac ctggtggtca tcaacgcggg ttcaaaagaa cgccaactcc
    61021 acctcgatct aactcaaaca ggcgtgtctt cgcgcgcggt cgaatcgctg tttggacttg
    61081 gacgggcgac gctggaaggc ggctctctgt gtattgagca cctgccttca aggcaaggag
    61141 ttgtatatag gttggagtag acgcctgaag atgagtacga taaaccgccg gtgaattact
    61201 gaatatccgg cggttttatt ttatagagcc ctttcatttt atgacctgcc gcaacggtta
    61261 gtgcgaattg tgtggcatgg taaaatacat ttccgattca tgcttgatga aacctgcata
    61321 ttaaaaaata aaagagttag ggtattgccg catgtcagat tccaacctga atggtcaatt
    61381 gaaagtaaat tcacccaggc caaccattga gatcgttttg cccgacggac gggtttacgc
    61441 tggtaatcgg ggtgcttcac tgtgtgaact gcttcagatg ctcccggaat gggataaccc
    61501 tcccatcatg ggcgcagtgg tcaacggaga gctgaaagag ttgaccgcca cgctcgaaat
    61561 ggacgcgcgc gtgcgcctgg ttacgatgaa ggatgacgat ggcgccagaa tctaccgacg
    61621 ttcgatcact ttcttgctcg aagccgcttt cgaagagctg tttccgaaaa gcttgatggc
    61681 gcttgaccat tcggtctctg ctggcgggta ttactgccag gtcagcaagg gaaaccagtt
    61741 gggcagcgag gacctttccc gacttgagct gcgcatgcgt gagcttgtgg aagcagacct
    61801 gccttttgaa cgccaactgg tgccgttgga cgaagccatc tcttactttg aatccaaagg
    61861 cctaaaagac aaagttcaac tgctgaaata tcgacaaaag gaatacctgg ttctgtatcg
    61921 gctgagtgaa catcgtgatt atcaccacgg atacatggtg ccttccaccg gctacctgaa
    61981 gtggttcgca ttgcagcaaa tgggagaggg attcgtgctg cgcttcccga gaagggaatc
    62041 tcccaagcaa ctttcgccca tgcccagcta ccccaaattg ctgagcacgt tcaggcagta
    62101 tggtaactgg cttcagcgac tgggaatcgg gtcagttggc gagctgaacg cctctattga
    62161 cgcaggcaat attcgagaaa ttattttagt gtctgaagcg ctgcataacc tgcaaatcat
    62221 cgatatttct caatctatcg cggataaatc tgaggaagcc cgtatcatcc tcatcgccgg
    62281 gccttcttct tctggcaaaa cgactttttc caagcgtttg tccatccagc ttttggcgca
    62341 gggtttctct ccctttccga tagagatgga caactatttt gttgatcgtg aaaaaacgcc
    62401 gaaggatgaa ttcggtcagt ataattttga agctttaggg gctctggata ctcatttgct
    62461 ttctgatcat ctaaagcgtt tgattcaagg cgaaaaagtg aggttgccca agtatgattt
    62521 tcacaccggc aagcgccagc aaggcgaaga aattcaactg cgccatgacc agatgatcat
    62581 actagagggc attcatggct tgaacccgca attgctgccg gagattgacc catcctcgac
    62641 ctacaagatt tacgtttcct gcctgaccca gctcaacctg gaccgctaca accggatttc
    62701 taccaccgat acccggctgt tgcggcgtat tgtgagggac gcgcgtgaac gaggatacac
    62761 cgcccaggag acgatttctc gctgggaatc tgtgcaacac ggagaaaagg aatacatttt
    62821 tccgtaccag gagaatgccg ataaactgtt caattccgcg ctggtgtatg agctttctgc
    62881 tttgcgcctg cacgccgaac cattattgcg ccaggttcca tacgggacta aagaatatgt
    62941 tgaggcgaag cgcctgttgg cattcctgga atggttccta cccattgact cccgcatgat
    63001 tcctgacaat tcattgatgt tggagtttct gggcggttcg attcttaaag attttatact
    63061 gtggaaatag cagaaaagaa cgggttcaga accgctgagc ccgttctttt tattaagcga
    63121 tttttttctt aaaagccctg cccaggcggg caattccttc gttaatccgt tctggtgagc
    63181 aaaaagagaa atttaaacgc atggtgtttt ttccgctccc atccgggtgg aagaactctc
    63241 cggggacata agccaccttc tcctgaatca cttcaggcag catttctgtc gtgtcgaatt
    63301 gttcaggcag ggttacccat aagaaaagcc ctccatgagg tcgtgtccac ttcactcctt
    63361 cgggcatgtg ttcttccaag gaatcgagca tggcgttgcg gcgttcccgg tagacttttt
    63421 ggatgaccgt gatgtggcgg tcaatgaagc catgctgccc gacctcgtgt gcaacaatct
    63481 gattgaaagt ggaggtgtgt agatcgcaac cctgcttggc cagcgccaac ttgctgatca
    63541 ctccatgtgg ggcgatgacc caggctaaac gcaaccccgg cgccaggatt ttggagaagg
    63601 tgctcaggta aatgacgttg cccgtatagg tgccattttg aacgcgcatt tgcgaatcga
    63661 gcagttctac cgcgggcagg ctttcgcctt catagcgtaa ctgaccatag gggtcatctt
    63721 ctacaatcgg tacgccatag cggtctgcta ggtgtatcag ctttttccgc ctttccaagg
    63781 agagagttgt gccggtgggg ttttggaagt tcggcagcac ataaataaat ttcgggccga
    63841 tgcgcagagc tgcttccagt ttctcggtga tcatgccata ttcatcaact gggacggtaa
    63901 tgtattctgc tccgtatgcg ttccacgcct gcagtgcacc caggtaggtg ggtgattcca
    63961 ccaggatgcg gtctccgcgg ttgatgaata ttttccccaa caggtcgagc gcctgttgag
    64021 acccgctggt gatgaggatg ttgccaatgt caacattaat tcccaggttg atggcatgcc
    64081 tcaggatcat ctcccgcaag ggaacatagc catctgtccc accgtactgg agtacttcag
    64141 ctcccttgtg ttcaaggacg tagttacacg ctttcttgaa ctcttcaacc ggaaatacat
    64201 caggcgctgg aaaacctccg ccaaacgaga tcatgtctgg ttgttctgtt accttgagaa
    64261 gttcccgtat ggccgatgct ttcatacgct gtgtgcgctg cgagtagcgg tattcccacg
    64321 gtgtctgcat ttttgtgtgc atttttcctc ttggttttta gaatgatttt tgccttacaa
    64381 cctggcaaaa cgactttcaa cagttgcccg ggagggcagg gttacccgtt ctcctggatt
    64441 caattgggaa ggcgccttgc ctgcctgaga cggtgctccc tggtaaatta aaatgacggt
    64501 gccttcaact gcgtacctgg tttcaataaa agcctgacca gtgagcaaac cgtctttatc
    64561 gatcgcgcaa ctggtcacgt gaccaatgac cctgcctttt tcatccagta ccgggtcgtt
    64621 attgtgcgcc atgcgcacgc ctttttcggc aaagcggaaa cgaacgacaa ctccttttcg
    64681 ggtaagttcc ttcttaatga aggattccct tcctataaac cagggtttgt aggtttttac
    64741 ataggaacca aacccggctt cagcaacgcc caggttgcgc tcaccaccca tttcatgccc
    64801 atacaggggg aggccggctt ccgtgcgcaa agaatctcgg gcgccgagac cgcagggctt
    64861 aagccccaaa ggttcaccaa ctttcatcag gtcgttccat aggcttgaga cgttttcagg
    64921 gtgtacgaaa agctcaaaag ccattttctc accggtgtaa ccggtacgcg agataatcag
    64981 atcgtacccg ccaatcaccg cttcgcaaag ttcagtgcgt ttgagggctt ttacttttct
    65041 ctgcgtggcg gcatcacaac ccaacgccag caagatgtcg cgtgatttgg gcccttgcaa
    65101 cgcgatatcc acgcgcatat cggcaccttc gcgagggtcg cgcaggttgc gtaaaaccac
    65161 cccgcgacca aaagctttga cccaggggcg gtgattgtcg accgctacac tggcattgcg
    65221 gacggcgttc agccaggccc agtctttcgc atcattcgag gcgttgacca caacaaggta
    65281 tttatccgcg ctgcggcggt agacgagggt gtcgtcaatc acattggcat ccgggtcaag
    65341 aaaatgcgtg tagcaagaat ccccaatctc caacgaacca atatcatttc cgcaaactga
    65401 atccaggaag agagccgctt gcgacccttc tgcctggaaa acgcccatgt gagcgacatc
    65461 gaaaagtccg gcggcgttac ggcaggccag gtgttcttca actaccgagg tataccagac
    65521 cggcatttcc catccggcga aaggaatgag tttggcaccc agtctcttat ggtcttcaaa
    65581 aagaggggta cgcatgagct cgttggaaga cttttcctcc catttaaacg atggaagagc
    65641 gttgccgttt gaatttgtta taccgatgta ccaggctttg ttgtttgcca tttctttacc
    65701 cgagctgggc attggcgcga ccaattctga ttcttccacc acaactgggc cggggatacg
    65761 cgcaggcagg tcgttatcaa aagcgatgta accatcagac agggcgcgca agaaggttgc
    65821 agccagacca aaattttctg cctggaagga aaacctgtag aggttatctt ccactctggt
    65881 aagtgaacct tccacttttt tcagcggggt gatgagcgag gttggttggg tctgaccgtt
    65941 ttgcaaggct tccacatcgg agctaaagca atagttcaca aagtacagaa cccggtcacc
    66001 tttaaggtcc aatgaaacca gtttcccctt gaaccggggt tggtcatcca ggtagtaaaa
    66061 atgcgggtag ccgctgttgc catgcgggag atcagttgca gcgctggcgc aaaggttgcg
    66121 gaccttgatt ttcgtttgct ctagaacatt aaaatcaatt ttggcgcgcg gtgaaggacc
    66181 ttttcgcatt tcaatggtgt acggcgtaat cgcctgcaag agatcggcaa taaggttgcc
    66241 aatttcgcgc atattttccg cggtcaaacc gcgttgagtc atccagggcg tgccatagcg
    66301 gatgcccgaa gcattgaggg cggttttatc ccctggaatg gtgttgcggt tgagcacaat
    66361 tcccgcgata tccaggatgc gggcagccat gtcaccgctc agagtggtgc catcaggacc
    66421 gacgatagtt ttgcaatcga tgttgcccag gtgagagtca gtcccgttga acgggatgcg
    66481 cagcccgcga gccttcaatt gagcggtgag ggcgacgcaa ttttggatga tttgtttttg
    66541 taactgctga aactcagccg ttgcggcgat tttaaacgtt gtggcgagag cagcgaacac
    66601 atgcacatga ggtccgccct gctcgccggg gaaaaccgct ttgtcgatct tctttgccag
    66661 cgcgctgtct gtagtgagga tgcatgcgcc gcgcggtccg cacagggttt tatgcgtggt
    66721 aaaggtgatc acgtgagcgt gaccgaccgg agagggaatg gctttcccgg cgatcaagcc
    66781 agcgatgtgc gacacatccg caagcaggta tgcgccgacg gcgtctgcga tggcgcggaa
    66841 gcgcgaccaa tcaggaatcc agggataaga ggaataaccg cagatgatga tcctgggcat
    66901 gacttctttc gcctgcgctt ccagcttttc gtagtctatc tgttcggttt ccgggtcaac
    66961 attgtaatga gcgaccttgt aaagttttcc ggaacggttc acagatgaac catgtgttag
    67021 atgaccgcca tgcagcaagt tcatgcctaa gatggtgtca ccaggttcga tcagcgcatg
    67081 gtacaccgcg ttgttggctg gagcgcctga gagtgcctgg acattgacat aaagttgatc
    67141 tgcggtgacc ctgtcagtgg cgaaaacctc cgcacagcgc cgccgcgcca gcgcttcgac
    67201 cacatcagca tattcgaccc ctttatagta acgaggatca gcgtatcgtc ggtaatagga
    67261 taaacggact ggatagtcca ggatttcttt ttcactcatc aaacgggttt cttcgtcggg
    67321 ataaccttcc gcgtataggt tttggaaaac ggaggagaga gaatcacgga cggcttgcgg
    67381 cgcggtgctt tcagagggga tgagaatcag tttacggaac tgtctttcaa cttcgtaatt
    67441 aacgaggtta aaaactgcgg gatcgatctc cgacagagat cctcgtacca gatagtcagt
    67501 catgtaataa actccttctt gatgtgagtg ggaaaaaata aaaaaccacc ttcagatgtt
    67561 ggccatacaa gcatctgggg gtggttggaa tggagtaatt atgagtcatt cgtttctcag
    67621 taaccgatct aaaagcacga tcttgatttc aggttctgta tcgcaacgat agtgacaagg
    67681 ttattttagc cttaacccct ctatgggtca agcaaaatct ggaagtgata aaaaggggag
    67741 gcattgccta ccgttcaaag cagatacaca cgcaagacga tcgtgtgccc tgatgtttat
    67801 cacgcttttc gcccttataa taaagaatct gccggaacaa atgatggttg agaaaagccg
    67861 atcaatggaa gtgaggacgt gattacaccc gaaaacacaa tcaatgaaca tccggaattg
    67921 gattacagcc tgaaaaaggc caggaagatg ggcgttattt tgttgatatt ttttactacg
    67981 ctctgggtat tgtctctgac cgggttaaac cttttcctgc gctggtctat tgaacaaagc
    68041 atgtttgaaa cagaaacagg catacaggat gttcgctggt tggttcattt agtgtgtggt
    68101 gttttcttgt ttatcccgtt gctggctttg tccttcgcag tgaaagttcc acgcgtcaaa
    68161 atcatgttgc gtctctggac ggtgggcgcg gcttttgtgc tgctttcagt cccgcccaag
    68221 gtgctctatc tcaccgcgca ggttcagtcc aatctgcttc tttgcggagt cctattactg
    68281 atgaccctgg tgttgttttt attaaggaat aagcaaacga gtggatcgaa gacgcttcct
    68341 ccatcagggt tggccggacc ggttttttta gcctgcgcgg ccctggcaac accctgggtg
    68401 atgtggggcg cgttgggttc cgtggaggac tcgctttccg cgttgctgct cggcgcagca
    68461 tttgcgcttt ttttcatcaa cgcggtcgta gtggattact tcgacaagac tcagccccct
    68521 ggcagcctgg tgaacaggaa ggatttcctg tttgacggct ttgtgttgtt catttttttg
    68581 tttgtcgttg tcagcgggct aagccagaac ggatctcaaa ttgtgctctg tctttccatc
    68641 cccgcttgtg ggtggttgtt ggcagcgcta tctaccgcgg ggcgcaaccg gaaaaatcat
    68701 gcccggcttg ccacgggcct tgtttcaggc ctgatgctgg cgctgccgtt ggcgtttttt
    68761 gatatggatg aactggcatt gttcatctct ggaactgatg gagaagtgtt tttctgggca
    68821 aacagggcgg ggtggaacac tctgaccttc caaatcgcgt ttgcgctggt ggtgttgata
    68881 tttttcaaac aacttgagcg cgttcacctg ggcagacgta tcaacgcgat gctgctggta
    68941 ttaagcctgg cggaggtcct cgttctatat ttcacggcgg gacaacccgg tttttttggt
    69001 gaccgccttt tcgttgtcat gaaagagcag gtgaacccta cccaattcaa tgaaataaac
    69061 cctcttctgg agaaaaaact cgccctatac caggccctga ccgaaaccgc tgtaaccacg
    69121 caagaagaag tgaccaccca acttgcaaaa tggcatatcg actttacgcg ttattacctg
    69181 gtcaatgggt tagaggtaaa cacaggcgtt ttgatgaaga gcatgctgga aaaaatggac
    69241 ggcgttgacc gggtgctgga cagcccgcgc ctccgcccgc tccctcaacc tccgccgcgg
    69301 atggagggtg aaataactat tcctcctgaa gattctttgt ggaacctgca aatgatcaat
    69361 ctgcccctgg tgcatgaaca actgggtgtt tttggagagg gcatcatcat tgggcaaacg
    69421 gattctggag tggatggctc acatccggaa ttggcgggtt cttacagggg agccaaaggc
    69481 agcaacgata ccaactggtt ggatccctgg aatcatactg ttgcacccaa tgacctgagt
    69541 ggacatggaa ctggcaccac cgcgatcatt gtcggcgcga cgcgcggtat tgcccccaag
    69601 gctcaatgga tagcctgcac gaacctggcc aggaacctgg gaaacccggc tctctacctg
    69661 gattgtatgc agttcatgtt tgctccctat ccacaggggg gagacccgct tctgcatgga
    69721 aagcctgaac tgggagccat ggtcgtgaat aactcatggg gctgccccag gattgaaggc
    69781 tgcgatgcgg atgtttttaa gccagcggtt gaaatattgg aagccgcagg gatttttatg
    69841 tctgtttccg ccgggaataa tgggtacttt gggtgttcga caatctccga cccgcccgct
    69901 ctttacgagc atgttttctc cgtcggctca gttgacagcg caggagagct ctctgccttt
    69961 tcgagcaagg gtcctgttac cagtgatggt agcgggcgca gcaaacctga cttgctggcg
    70021 cccggagagc aggtattggt tgccgcacca gggggaacct atacttacag cagtggcact
    70081 tcttttgctg cgccacatgt gactggtgtg gtcgctttga tgtggtctgc caatccaaaa
    70141 ctcatcggag aggttgccct caccaggcaa atccttcaag aaagtgcaca accctacaca
    70201 gacgggtcca ctagttgtgg gaccccacaa aatgaaagcg gcgccggcat tttggacgcg
    70261 tacgctgcgg tcaaagcagc gattgcggta aaatgaatga aaggattgaa ccttttcttt
    70321 ggaaaaaggg ttaaatagtt gaagaaacaa agcgctgcac caacagagtc attgaccgat
    70381 cagaacgctg ttcaacttgc ctgtaacggc aacgcagagg cgtttagcta cctctatgaa
    70441 cagaacgtga agcgcattta caattacatt ttttatcgaa tcggcgctga agcggacgca
    70501 gaggacctga ccgcgagagt ttttcaccgc gcattcgcgc acattaacag ttatgttgaa
    70561 aaaggagtgc ctttcactgc ctggctgtat cgcatcgctc ataacctcac ggccaactgg
    70621 taccgcgaca atcatcgaag gcgagaagta tcattagatg accattttga tttacctcac
    70681 ctcagcgatt tacctgaacg cactattgaa atgaaccagg aaaaggaact gctcctgaaa
    70741 gccattcggc gattacctgc tgaccgccaa caacttattt tgctcaagtt tcttgaagat
    70801 ctttcaaacg gtcagatcgc cattattatg gggagaaccg aaggcgcaat aaaaagcctt
    70861 tatcaccgct cgctgatctc gttgagggaa gaattaaaac ggatcagcgg cgttgagaca
    70921 tcggattttc gataaggggt atggaatatg gaacatggcc tagaacaaaa tggaagacct
    70981 ggtttatggg ggttggagca aaagctctcc aacctgctta aaccagtgac gcctgaccct
    71041 gaatttgtcg actcactaaa aatgaagttg acccgcgctc cggccgtgtt agttgaaacc
    71101 agccggcgta aaatggggct tctggtggtg agttttggat tgttcgccgg tgcttttttg
    71161 gtttggcttt tgcgcaaacg atggcagtga gcgctgctta taaccctaat ttttctttta
    71221 attgataagt cgagggctcg acgagtattg cgccatcagg gaagacgatt gttggcacac
    71281 tgcgaaatcc gttcgctacg ctttcaacga acttagccgc ggtctcatcc tggtcaatat
    71341 cgatccactc atagtcgatc ttttcatctt caaacaacct tctggcgcgc ttggttgaac
    71401 cacaccaggt tgtgccgtac atcataatct tcttctgcgt atcacccact tactctgata
    71461 cctccttcaa taaagtttgg atcaccgcct gcgcgtcagc gagcaggtca atcccggttg
    71521 gtagggcgta attttcatcg acccgcatgg tcaggtgttg aagggcagga aggacgctcg
    71581 cgggcaggct ttttcgcaca gactcatcaa cgaggatctc gaaattgttc aagcctgcag
    71641 cttcaattcc atgttgatca aggaaatccc tgacgacgat gccagcggcc cgccgcgcgc
    71701 ataccctggc cattccttcg ttgccttgtt ttcttgcagc gaaggccatg tccatttctt
    71761 tctgaattct ctcacttatt ttcatggggc aattctaaac catttcgcat cttttgcggc
    71821 agggttctgt tttcgtttcg tggcttatgt taaaatcaaa cgcatgagcg agaaaaccgt
    71881 cttcatgggg tcacccgaat tcgccgcgag catccttgct gcactgggaa accactatga
    71941 cgttcgtggt gtggtcactc aaccagacaa gcctgcaggg cgcggaaaaa aactcactcc
    72001 ccctccagtg aaaacgctgg cggagcgttt gggttaccct gtcattcagc cggaaaaaat
    72061 gaaagaccca ggtgtgtttg aacaactgca tgcctggaat cctgatttca ttgttgtggc
    72121 ggcatttggc aagattctcc gtcaaaatgt gttgaacctt ccgcggttag gttgcatcaa
    72181 tgtgcacgct tcctacctcc cgcgctggcg cggcgccgcg cctgtccagg cggcgatcat
    72241 ccatggtgat actaccaccg gggtcagcat catgaaaatg gacataggag tggataccgg
    72301 tcctatcctg attcgcgagc gggtgaaaat tgaacccgct gatgatgctg cttcactaac
    72361 cagccggctg gccacggtgg gggcgaccct gctgctacag gcactggatg gatacattaa
    72421 tggcaacctg tcgcctgtac ctcaaaatga gaacggcgcc acttacgcgc cgatgatcaa
    72481 taaagaggat gggttactgg atttctcaca ggcagccgag atgctggaac gtaagattag
    72541 agcgtatagc gaatggccgg gcgcctacat acggttgggt gaagaagcac tgaaggtccg
    72601 ccgggcgaaa gttgtgcctg gcagcggggt cccagggcag cgcgaaacct gtaatgggta
    72661 tccttgcatt cacacaagcg atggcaaact tgtgctgctg gaagtgaaac ccgctggaaa
    72721 gaactggatg aatgccgcag actacctgcg cggttcccgc tcctggattt caaactgatt
    72781 gattatttag aacaaaccgc cgcagagtat tgtgctcaat gggacagcgc gctgatggtt
    72841 agcggggtga taggaagact gatggttctt aaacatcacc gttaggaatg tattgggtta
    72901 gaacctcgaa atggcgattg tgggtattaa gattcctcag cggataaaaa acgcctgcgc
    72961 cactcgtaga ggatgttcag ggcttctatc ggtgggaggg tgttgaggtc gatggctttt
    73021 aactcatcca acaaagggtt tgtttcgggg aaaagggtta actgctgtga ggaggggaga
    73081 tctactttaa tggtggaacc tgaagaagct tcgagttgct gtaggatctc actggcgcgc
    73141 gaaattaccg ggcgcggaat gcctgccagt tgcgccacgt ggataccgta tgaacgatca
    73201 gtgccgccgg ggacgatctt gtgtagaaag atcacctggg tgccattttc agctaccgcc
    73261 acgttgtagt ttcggatgcc tggcaacaga tcactcaact gtgttaattc gtggtaatga
    73321 gtggcaaaca gagtacgggc tcgcaatctg gggtgattgt gtatgtattc aatcaccgac
    73381 caggcgattg acagcccatc gtatgtggat gtaccgcgtc ctatctcatc cagaatgagc
    73441 aggctgcggc tgctggcgtg gttgagaatg ttggcggtct cgatcatctc aaccataaaa
    73501 gtggattgac cggtgtggat ctcatcctgg gcaccaatgc gagtaaaaat ccggtcaacg
    73561 atgccaatgg tggcgctggc agcaggcacg taagacccaa tttgcgccat taacacaatc
    73621 aacgctacct gccgcaagaa ggttgacttg ccgctcatat tggggccggt gatgatgcgg
    73681 ataatctcgc ctgtttccat cacggcatca ttgggcacgt atcgctcact ttgcagcgtg
    73741 gtttccacga cggggtgtct accatcgaca atgttgagca tgggttcttc tgctagaacc
    73801 ggacgaacgt atgcatgtat ggcggttact tcggccaggc tgctcaacac atccagctca
    73861 cccagcaggc gagcgctttt cagcaaggca gataaatgcg gcagaagccg ctggcaaacc
    73921 tctttgaaaa gccgattttc aatttccctg atgcgctctt ccgcgttcaa taccagcgct
    73981 tcatactctt tcatctcagg cgtgatgtat cgttctgcgt taaccagagt ttgttttcga
    74041 atgtattctg ctggcacctg cgcggtgttg ctgtgggtga tttcaagata atagccaaac
    74101 accttgttgt agccgacttt gagggatttg atgccggtcc tctcacgctc tacggcttcc
    74161 aggttggaga tccactctcg cgcgtggttg gacatttcca ccacctgatc cagttcttct
    74221 gagaaaccaa ccctgatcac gccggtatgc tgcagtgtgg ctggcggttc atctgcgagc
    74281 gattgttcca gtaagttgag aacttcaggg caaaggtcaa agtcgattaa cttgtgagag
    74341 agctgcaaga cgtcttgcga aagggtatcg cggatggctg gcagtttctg caggatggca
    74401 cggatggcag ccagatctct tgggagagcc acgtttccgg taaggcgatt tgccaaacgc
    74461 tccaaatcgc caaggtgctg caactgcaca cgaaaatcag cgcgaaccaa tgaatgctgg
    74521 tgaaaaacag caacaatgtc caatcgctca ttaatgctat caatccttaa caatggctta
    74581 ctgacccatt gccgcatgca acgatgcccc atgggagtta aggtgtggtc aaggattgaa
    74641 agcagcgacc cctgagtttc gccgcggcgg atggtttcgg tcaattccag gtttctacgg
    74701 gtggccgcgt ccaatgtcat aaattcgccc gtgctgtaag tggagatgct tttcaccatg
    74761 gataacgcgg ctggttgggt ttgttccagg tactgcaaaa tggcgccgct ggtttgaatg
    74821 gcggaaggag cattctccag gccgtaccct tctaacgtgg tcacttgcat tacccgcagc
    74881 aatacctccc ggcagcgcga agtttcaaag cgccagtctg gaaggctggt aatatgacca
    74941 gaaaacgctg tgttcagttt gagcgattcg ggcaccaata tttctgccgg gtccaacctc
    75001 gtgagttctg cccgtaaatc ggataatgga tcacgcgatt cgattgtggt gacagcgaac
    75061 tcaccagtgg tgatatccgc gtaagcaacg ctcaactggc catcttctac acgcgccgct
    75121 gccaggtaat tattccggtt gctgtgcaac aatcctggct caatcactgt tccaggcgtt
    75181 accacgcgca cgacttcgcg cgggaaaatt cccttggcgg gttgttcccc cacctgctca
    75241 caaatggcga catgatagcc tttatcgatt aaccgggcga ggtagttttc agccgcatgg
    75301 taggggatac cagccatcgg aacgcgttgc cccttggcca cattacgaga ggtgagcaca
    75361 atatcgagct cccgcgaagt gatttgggca tcatgatcaa aagtttcgta aaaatccccc
    75421 aggcggaaga acacaatcgc ttcaggatat tgctttttta tatccaaata ctgctgccga
    75481 ataggggtaa catcttctgc catgaatcgt ccaaatcaag tcgatacgat aaaacttaac
    75541 agatagtaaa tggaaaagaa aatggattga gggttacaac ttctgcccgc attcgccgca
    75601 gaacttggca tcgggaagga tttttgaccc gcaatgtggg catttgtctt tcggtttcaa
    75661 ggcgtaaccg cactccgggc aaaatttcgc gttggctgct aatggtttct cacagttggg
    75721 gcaggtggcg gtgatccctt cgcgccaata ttcagtgccc agttttttat cctcctcagc
    75781 catggctgca tgagcccaga cttcctcaac ggattttgaa gcctgagcag ccgccatttc
    75841 cacgcccagg tctggagcgc agtctttgca caagcctttc tttttgttcc aacatttttc
    75901 acggcatacc caggcgttgc agcgcgggca ttgaatgaaa tatggtcgaa tttctttgac
    75961 cgcttcagtg aagttttcgt catgggctcg ttcccaggcc gctgaatgaa cgcgatcact
    76021 gatattcgcc gcctggctga ggaaccctcc aaacaagctg ctggcagtat ccaatgcccc
    76081 gctcagttta cctgtcaagg aaggtttgaa ggttgtacgg tagcctgatt cgcagcgggc
    76141 gcaggtaaat tcaaattgaa aacccctgtc agtggacagg tcagtgtagt tcttcacgaa
    76201 ttcaaggtgt tcaaccatga taccctccca taatcgaaaa tgagtaaacc ttattaatta
    76261 tatcccaaat ggtttgggcc ttctaaagtg gttataattc attttattac aattgaggat
    76321 caagatgcaa agagttcaga catgcgatat ttttacaagg attcaattgg ggattggcac
    76381 gtgggcctgg ggagataaac tggtttgggg gtatgggaat agttattccg aagcagatat
    76441 ccgtgaggct ttcaaggtat gcgtcggtgg tgggatacgg ttttttgatt cagcggaagt
    76501 ttacggtcag ggaaaagccg aaaccatcct gggcgagctt tgcgcccagg aagatgaaga
    76561 atttgttatc gcgacaaaaa tgatgccatt tccctggcgc ctttttcata attcattgaa
    76621 attcgcgctg cagaacagtt tgcgccggct gcgggtgaac caggtgcagc tttaccagat
    76681 gcattggccg ctaccgccgg taaaggtgga tacctggatg gatcggatgg cagaagtggt
    76741 ggatgcgggg atggtcaaag cagttggtgt ttcgaactac aacctggaac aaacgataac
    76801 cgcacacgaa gcgcttaaga agagaggatt gcggctggcc tcgaaccagg tagagtacca
    76861 tttgcttgag aggcgaattg aacaaaatgg gttgatggag tattgcctgg aagaggggat
    76921 taagatcatt gcgtatagcc cgttggctat gggtatcttg accggtaagt atacgccgga
    76981 aaatccaccg gctggcacac gagcagccca gtacaatcgg tcttacctgg aaaagataca
    77041 gcctttgata aaaacgttga cccgtatcgg caatgacgct gacggcaagt cggccgggca
    77101 agtggcgatt aactggtgca ttcaaaaagg cacattaccc atcccggggg taaaaaatgc
    77161 tgcccaggca gttcaaaatt tgggggctat cggctggcaa ttggacgcag aggaaatggc
    77221 gcttttggat gaggtcagcc ttgcagtaac cctaccctaa gcaagggctt gtttactcaa
    77281 caaattccag ttcctggtcg tccaatgtag aaaattcacc ttctggctgc agagaaatct
    77341 caccgaagag ctgattttgg tcggcgccga ccatatgcct ttttacgagt tccagtaaag
    77401 ccaggaaggt gaccaccact tctactcggc tggagcgggt ggtgatgagc tgttggaagg
    77461 ttgtagcgcc cattttttgc agcgcttcga gaatgacatg aatcttctga cgaatcgtga
    77521 agcgcggtga cgcgaccact tcactcaaag gcggtaaatt attaggattt gaaaagacct
    77581 ctcgagccgc ttccacaagg gcttcgaggg tcaattcact gatatccagt ttagagggaa
    77641 tttgtaccgc tgggttcgcg tcgaggcgca aataggtgtg atagcccgct tcttcgcgag
    77701 attctaaaaa cctggacagg tctttgaatt ttttataaac aaggagctgc cgtgccagtg
    77761 cttcgccggg atcttcttcg acctgcaagc tgggaatggt ttgccttggt aaaagggcag
    77821 cggatttaat ctgcaccagc ttggcggcaa ttacgagaaa cgcggagact tcagccgggt
    77881 cttgagtttc gaggtgatgc atatactcaa ggtactgatc ggtcacctgc gccagagaaa
    77941 gacgggtgat gtccagttcc gccttttcaa tcaactggag taggagatcc agagggcctg
    78001 aataaacctc ggtgttgatc ttatatcctt ctgtttgatg tcccgcgatt tgaaagtcca
    78061 cgccggttat tatagcagat tgcggtcagg catattctat gctataaaag cagggaacga
    78121 gatacccatg atgaagagcg ggacgattaa ttgcacgtat tgcggtgagc cttatccatc
    78181 cgcagggcta cctttccgct gtacttgtgg gggtatctac gatttttctc gtttccctga
    78241 tttcaccatt cccaaagctc aggcggtacc tcgcggagta tggcatttca aggattcttt
    78301 agggctgccc gctgaggcgc ccatcgtatc gcttggcgaa gggaacacac ctttgatgtc
    78361 gtggtctctt catcatcgac aggtttatct caaaatggaa tgccagaacc ccactggttc
    78421 ttacaaagat cggggcacag ccgtgctgac cagttttttg ctggcgcgtg gggtaaagga
    78481 ggtggtggaa gattcctctg gcaacgctgg cgcttctctg gcagcctatt gcgccagggc
    78541 ggggattaaa gcggcgatct ttatccccga gtcagcgtct gggcccaaac gctggcagat
    78601 tgagagatat ggagcagagg ttcaccgtgt tccgggccca cgagacaacg cggctgaagc
    78661 tgtattacgt gcggcaggcg ccggaaaagt gtacgccagc catgcttata tgccatttgg
    78721 cttgacgggt atcgctacga tcgcttacga aatttggcaa tcacttggca gagaacccgg
    78781 aagcctgatt tcaccggtgg gtcatggtgg cttgctttat ggggtaatgc gtgggttcga
    78841 tgcgctcttt tcagccggat tgattggcaa gaaacctatt tatattgggg ttcaagcaga
    78901 aaactgcgcc ccgctgtttc aggcttacag gcaacaaaaa ctaaacccgg ttaaagtgga
    78961 aatttctgcc tccctggcag agggaacaca tgtttcttgc ccggtgcggg gagcccagat
    79021 actggaacga atgcaaaatc accaagggag aatggtaatg ggctctgaat cgcaactggt
    79081 agcgctctat cggctctcag cacgatcggg ttatttttgt gaaccgacct cttgcttatc
    79141 ccttgtgccg ttactagatg ataaaattga tcttgttgaa ccggttgtgg ctatcatgac
    79201 aggctcaggc tataaaacaa atatcatctt acagtagaat aatgtgatcg cgagctcctc
    79261 tgaaaagaga ggttggcgct ccccttattc gtggagaaaa cgcttgactc aaatgcataa
    79321 cgagtgtaac agagaaaccc ccgaagaact ggctgccaga atctttcccg acttgaccga
    79381 tatcctgctg ttaagcagag cattgaccca tcgctcttac ttaaacgaac atcctgaggc
    79441 tttagaggac aatgaacgct tagaattcct gggtgatgcg gttcttgatt ttgtggtggg
    79501 tgactggtta tataacaggt accctgaaat gccagaaggc gatttaacac gcatgcgttc
    79561 ggctttggta cacaccgctc aactggcaga ttttgcgcgt agcattcaat tgggcagggc
    79621 gatgcgactg ggcaaggggg aaacccaggc gggcggatgt gagcgcacag ggttgctgtg
    79681 cgacaccttt gaggcagtcg ttggagcaat ctacctggaa atggaggttg aaggcgtcaa
    79741 acgttttatc taccccatgc ttgaatcctg cgctgatgac attctgatta atcacaaaga
    79801 tgaagacccc aaaagcaagc tgcaagaatg ggcgcaagga aacggttacc cggcgccggt
    79861 ttataccacc cgtgacgttt caggaccaga ccactcaaaa ttctttgtcg tggatgtgat
    79921 cgtaaacgga aaaacctgcg gcacaggttc aggtaccagc aaacaggctg ctaccaagca
    79981 ggcggctgca gacgctctta ataaacttca cctttcagat tggaaaccaa ctcagcgatg
    80041 aaaccgaaac taagatcact ggagctgcat gggtacaaaa ctttcgctgg cagagcgtta
    80101 ttcgagttcc cggggaacat caccgcgatc gtcggtccaa acgggtcggg aaaatcgaac
    80161 atcgcagacg ctgtacgctg ggtgctgggt gaacaatcgt atactttgtt gcgcggcaaa
    80221 aagacagaag acatgatctt tttgggttct gaccaacgcc caaaagcagg aatggcttca
    80281 gccacgatta cttttaacaa cgaagaaggc tggctgccga ttgactacag tgaggtcgcg
    80341 gtcgcccgaa gagcgtatcg tgatggcagc aacgagtact tgttaaaccg gcagcgagtg
    80401 aggcttaaag acaccacaga actactggct cagtcaggct tggcggagag aacctatacc
    80461 attatcggcc agggcctggt agacgctgcc ttgtctttac gccctgaaga acgccgcaaa
    80521 ttctttgaag aagccgccgg aattggacta ttccgatctc gccgagagga agcgatcacc
    80581 aggttggatg ctacccgccg caacctggaa cgggtgacgg atattctggg tgagcttgaa
    80641 cctcgtctgc gcagccttga acgccaggcg aagagagtgg aagaatatga ccaggtgaag
    80701 gatgatcttc aggtgttgtt gaaggactgg tatggatttc attggcaaaa tacacaaaaa
    80761 gaactgctgc gaaaccgcga ggtgttgaaa aaccaggaaa tcaaagttga agccgccagg
    80821 acaagacgag gagagatcga agtaaaagtc gatgcggcac gcttgcggct gcaagaatta
    80881 cgcagtctac ttaacgcctg gcatatggaa accgctgaat tgcatcgtca gcgggaaaaa
    80941 atcagtcgcg atatcgctgt actcgaagaa cgtttccgtg catatcaaaa ccaggatatt
    81001 tcactcaagg ctgagttgtc acgaaatgaa gatttaaaac actcgaatca agaaaaatta
    81061 acagcgttaa tggatgagga agaaaggatt aaccttgagc tcgcccagtg cgcggaacag
    81121 ttggaagaaa ttcgtatttc ttttgatgaa cgcaaagcaa atagagacca actggaaacg
    81181 aagataaatt tggcgcgcaa gcaactgttg gaagttgaaa cccggcaggt gcaattaaag
    81241 gcgcatcaac gcgaattaac tggacgggtt gaatcattcg aagatagccg gaaaaatctg
    81301 cttcagagta ttgaccaaaa tcagaacggg cttaataaaa ctcagaaaga gatggaagag
    81361 attagcgctg ggcaggtaaa aatccagcaa agtgcagaca tatatgttcg tgatatcggt
    81421 atagctgaag aaaaactggc tgaactggag cttcaggaaa aagaattttc aggagaaatc
    81481 agccgcctgg aggctgaaca tgcccgggcg aaagcgcaac tggaagtgct tcaccaggca
    81541 gaactcgcgt tttccgggtt gagtgaaggc gccagaagcc tgctcactgc cgcgagtcaa
    81601 gggaaaatcc ctgccggttt acggaccctg agcagttatc tggatgtacc gtatgaactt
    81661 gaaactgcaa tgagcgccgc gttgggcgac cagcttgatg cggttttgtt tggagaagga
    81721 gttgacccgg agagaatact ctcctacctg gaaaacgatg agtcaggccg cgcaatgctg
    81781 attccactca ctgcggctgg gaaaggccag gctcgaaaat ttgctggccc cgggatttta
    81841 ggtgtcgctt ctgacctggt aaaaccctct gacctaaatc gtggactggt gaattttctg
    81901 ctggggagtg tgattatcgt caacgatcgc gcaaccgcca ggcggctgct aaaggatttg
    81961 ccagtggatg gcagaattgt tactctcaag ggtgaagtat ttgatggcag cggcgtcatc
    82021 attgctggaa aagaaaatcg ggctgggatt atcagccgtc cgcgagaaaa gaaagacctg
    82081 acacagcagg taaatcgtgt tgccgaaaaa ctggctgaga aaaagaagga actggaagaa
    82141 cttcaaaaaa acattatctc tttgcgtaat gagaagagcg agcttgataa ggcagcccgg
    82201 gaagagcagg ctaaactcca acagatcaac gcaacccttg cgggaagaga acgtgaagtg
    82261 gcgaaactca tgcagcagct ggaatggcaa aataaccaaa tcaacacgct ggtcagccag
    82321 gccaaatccg ctgaaatgga tatggacggg atcggaaaag agatcgcggc gaatgagaac
    82381 gccatcattg agctgaatca aaccatccgg caggataacg aacagctacg gggcattccg
    82441 atcgatgaaa ttcagcaaca attggtgcac tggaacacca cccaggcggt gaccgaaagg
    82501 gcgctgcgag aggtggccgg gcgagtgaat gaatccaaac agaccatcgc ccaaaatcaa
    82561 gcggtcattg aacaggtaaa cagacgcatc gaagaggtgg tgaccgcaac cgctgaagtc
    82621 cgggcttcta tcgaggaaag caaaaaggca gaagggttgg ttaacttaag cctggatgaa
    82681 ttcagtcaaa aaattaatcc ggcagaaagt gaattgagga aacttgaaag cgagtattcc
    82741 gagttgcagc atgaatattc acagacccag caattcgagg ccaatgctga tcgggtatac
    82801 acacaggccc agttggagta ttcccgcagc cgcgaagcat tagaaggcct gcggagacgg
    82861 attgaagaag atttcggttt ggtaaccctc gaatattctg atgaggtcac tggacccact
    82921 ccattgccat tggaaggcgt gggcacactg gttgtgctgg atgaacttcc caagggactc
    82981 gaagattcga taaatcggca gcgcgcgctg ttgaggagaa tgggagcgat caacccggag
    83041 gctcgcaaag aataccagga agtcaaggaa cgccatgatt tcatgaaaac ccaactggcg
    83101 gatctacaga aggcggatag cgaccttcgc caaatcatca cagagctgga tgaactgatg
    83161 caccgccagt ttcggacgac gtttaatgag gtggcagcgg aattcaaaga aatgttctca
    83221 cggttgtttg gtgggggttc ggcgcggtta gtgatgactg atacagagaa tccgacggag
    83281 acgggaatag atattgaagc caggctgcca ggcaggcgtg aacagggact gtcactgctc
    83341 tccggcggcg agcgctcact cacggctgtc gcgctcatct tctcattact gcgcgtttca
    83401 cccacgccat tttgcatcct cgacgaagtg gacgcgatgc tggatgaagc caatgttggc
    83461 agatttactg agttgttgaa agaactcagc caggagacgc agtttattgt catcacgcac
    83521 aaccgtaata ctgtgcaggt ggcggatgtg atctatgggg tgaccatggg gcgcgattct
    83581 gccagccagg tcatcagcct gcgccttgac gaactgagcg acgaaatggt taaataaatt
    83641 gccaaatgaa aaccgcgaga atctcgtctc gcggtttttt tttataatct actgaccagg
    83701 cgcaattcga tattgcgccg gtatggcggg ggtattcggg acgaccgatg cccagacgtc
    83761 ttttttctca attttgatat ccgctttggc agcttcaatg aaatcagtga aggctttctg
    83821 tttcagtgtc tcccattggc tgtctgaaag agcgcggact tccttgccca acaactgaat
    83881 cacgtgaaaa ccaaagtcag attgaacggg ctggctgatt tcgccaattt tgagggcgta
    83941 ggctgcggtt tcgaaaggtt cgaccatctg tcctctagaa aaccagccca ggtcgcctcc
    84001 attgacgccg gaagaggtgt caatggagag ttcagcggcg atagttccga aatcttcacc
    84061 tttcttcaac cgttcgatca cagcattggc ttcttcttca gacgccacca ggatatgccg
    84121 ggcccagacc atttcctgtt cgcggggtat atccttggtc actgcttcgt aaaccttttg
    84181 atttaataag aggcttttta catacgcgcg aaaatccgct tcagtaaacg ggaactgttc
    84241 attgatgctg gtgagcattt gattgaaaag ctgtttgaaa ccatcgcggg tatacgcggt
    84301 aggcgtagga gggatctgcg tggcggtagg cacgctggtg ggagtgctat cttccactgt
    84361 gggggtggcg agcgatggat cagccgtcgc agtcggttca cttgtggcgg tgggttccgg
    84421 ggatggcgtg ggcgtaatgg tgatgatggc cagctgctgc gctgacatgg ttgatgtagg
    84481 caggtaggcg taggtaggtt gcggtgtggg cgtgccgtct gggaagaaac cgaagccacg
    84541 ttcaagttcc ttttcgatat cttcatctgt aacggagata cccatttcgg ccgctttgtg
    84601 ggccacaacc gcctcgttaa tcattttctc caatacatcg ctgccgaact ggatgtagtt
    84661 gtccagcgcg ttttgcatct gcagcatttg cgattggaaa aacatcgcgt atccagatgc
    84721 ggcgtaagaa gtaaagtttt caacataaga caagcgttca tactgcacgc gttttgcgaa
    84781 ctggcttgct tttatgttgg tatcgcctac cttcgccacg attttttgat tcttaagaac
    84841 agtcccttgc aacaccccga aacctaccag caaggtaacc actgccacga tcgcgataag
    84901 gacggtgatc atgatcttct tctggcgatt ttcacgctgc aacctggctt cacgcttttt
    84961 agaagcaggc tttggtgttc tctcgatgtt ttcttttgtc atttgtcctc tatatcttat
    85021 tcgagcgcaa tctgatgacg atgattgcga cgaaattgtg aatagcaggt attaggttct
    85081 gcgttacctg atcgactcgc tccaacgttc accggtgaac gggagaaggg cgatatggcg
    85141 ggcacgtttt acagctgatg ccagggagcg ctgatggcga gcgcaggttc cggtttgcct
    85201 tctggggcgg atcttaccgt cttcggtgac aaagcggcgt aatagatcgg tgtttttata
    85261 atcgatggcg atattcttgt ctgcgcagaa ctggcagatc ttcgggcgtg ctacaaaacg
    85321 gcgctgacca gaaaatcgtt cgctttcgtt tgtaatttcg tctgacattt tttactccat
    85381 tagaacggat attcatcaac cgcttgaaaa tcggtttcct gaaggtcccc gttgggttgg
    85441 gcattttctt tcttctcgcc caggatcatc atttccgaag ccactatttc cacattgctg
    85501 tgtttgactc cttcggaatc ttcccagcgg cgggtttgca ggcggccttc aacataaacc
    85561 tgttgaccct tagtgagata ttgtttgcat atttcagcca ggtttcccca ggagaccaca
    85621 tagaaccatt cagtttctga gtggcgttca ccatctccag tattccagga acggttggtg
    85681 gcgactgtaa aagttgttac aggtcggccg gaaggggtga aacgcatttc ggggtcgcgc
    85741 cccaaatgac caatgatcat aactttattt aaatcacgac ccattttcta actcctcatt
    85801 cttcctgcag cttttattac gctaccacag tgatcatata acggatcacg ggttcgtgaa
    85861 agcgcaggtt acggtcaagc tcagaagtga gggcggggtc catttctgca gtgagaagga
    85921 agtactgtcc ttcgcgctgt ttgcgaatga ggtaagcaag acgtttcttg ccccagttat
    85981 ccactttgga tatactgccg ccgcccgctg tgatccagcc ttttaccttg tcgagagcct
    86041 cattgagagc attctcgtcc agttccgggt ggaagatcaa tacgagttcg tatgtgcgca
    86101 tggtgattcc tcctttcctc gggtttgccc tcgaacagca ccgttggtgc ggtcaagagc
    86161 ggaaaggtag cagtaacttg ctgcgtacca gacgtagtat tttaccataa gttcttcgaa
    86221 atctaaggga attaatcccg tcatcttgct gggtacaaag attaagatgg tgatttgaaa
    86281 cgaaaccaac aggatagctg gttcaggcgt gagagcgttc tgcgagcgag tattaatagt
    86341 gggatgaaaa aaagtgattg gtcattctca ctattaatga gcgcttccaa ttattgttga
    86401 tttaatcatt cgttcgctta aagacaggag aaaccaggaa atgagccgta tttatggtaa
    86461 aatactgacc gttgttgggg gctcgtctaa cggcaggaca gctgactctg gatcagtaag
    86521 tagaggttcg aatcctttgc ccccagccaa agagagaagg gtcttcgaag tcgaaggccc
    86581 tcaattattt tggtgctgga aaaagacact gtgtatcttc ttgcatctct gtaaaagcat
    86641 gagcacctca atagagggtt ttagtgattc ctttatggac caacatcaga aatcaatttg
    86701 accagcgcgt tataccttct ggaccaatca tgttgtctgg caaattggat gtaagtatcg
    86761 gcgcgctcct ttattggctg ataattcatc aaagccgtca agaattcttc atatgaactg
    86821 cataaaagtg ccggcgatgc gatgtgttct aattcatccc attttgatgc caccactggt
    86881 aaaccacagg ccatatactc gtaaagtttc accgggcaga tcgcattgac caaagtgggg
    86941 aaatcccgct gattgaatgg aataatgccg atatccgcgt aggaaagata ggctggcagt
    87001 tcagaatgag gaactggacc ggccaaaatg aggtttttca ctttgataaa tcgcgaaccg
    87061 aaattctgat tgggaccaat tatgacaaag gtgaattttg gcagggcaat ggctaaacga
    87121 ttgataagat caaagtcgaa ccagaaatca attgagccgg cgtaaacgat caaagggcgg
    87181 gaacaagatc gatagagctc agggatggct ttcacttttt ggaaatgttc gaaatcaact
    87241 ccgtttggca ggaaaacggc ttgcttgggt tgtaattgcc tgacacgatt ttctaattga
    87301 tgagcagaat aagcaaccag atcaaccttg gaggcgatct tatgctcagc ttgtgtataa
    87361 aaagggttgt aggaagaaaa gccagagtcg ttatctgcca cccgaaatac tttctttttg
    87421 gcgttgatta aatccagcaa atacccctgc aaaggatcgc gaatatggag caaatccaca
    87481 tccaaaaaac cttttactgc caggtttttc ttaatgttcg ggaaaattgt cttgtgccag
    87541 tactgaaaaa cccatttttt cgccaaaaaa atctcgtttt tcggaatcac cgcggcaaat
    87601 ggtacaaaag aacagattat gccttttcca tgcgtatacc atcgcccatt ggacaggcag
    87661 gtggaaaacc gccttttaaa cgaattattg tctcttgcaa ccaaatgcca aggggtgatg
    87721 ggtttggaga taaaaatcac ttgccaacca ttggacgcaa attgcctggc taattgatgg
    87781 tcgcctactt gaataggcga ctcataatca acagacgtaa taaacacggc ttttcgaatg
    87841 gggtctgtca tctcattacg cgttctgcgt tctcaataaa aactggtgat aagttgatgc
    87901 attggacttc aggtcaacga gatccttata cttaaattac atacctgtaa caccgtcttc
    87961 gatcaattta cgggtcgccg aagaatcgga ggtaatagca ggtatcccat accttgcggc
    88021 tttctgaacc accgatccct gtgcttcata ccatctggaa ggaaaagaca atacgcgagt
    88081 aacgcttatt cttttctgaa tctttctgaa ggataaccag ccttcgaaga ttgacatggg
    88141 attaaatcct ctgtaatacg gacattaagg tgatgatacc tactggaaag aattataaaa
    88201 gaaaagcctt taattcgagc attttttcat cctttgcgtt tttgaaaagc ttcagacaca
    88261 tatgagacaa aaaagagcat cgagttcttc cttcacgggg acaatccaat aaaaggaaga
    88321 gtctcaagga aaagaaagta agtgaaaagg agatcaacct gctgctgaga ggatcaggct
    88381 cgaccgtggg gcgaatacgg gtagaccaga ggagaagaag agaaaaaagg agcaaaagga
    88441 gggcatgtgc catacgaaaa ccgaagaatc gtgtggtcaa tgaacctggc gacctggttc
    88501 aggcgggcgc ttcaatcatc tgtaaaaggg aacgcgtcta taaagggact cgccccatca
    88561 ggcactggac tatctcatcc cggccgagta tatttatatg taccacccca gggtgacttc
    88621 gaaaaaggcc aatatgaatt gaacaagacc aaccgcttgt caacgcaaaa caagatatgg
    88681 agggatggaa tgaaaaaact ggcgattgca ttaattctcc tgctggctac ccctgcttgt
    88741 tcgatttcga atcaacattc tgtggagatt caaccgggct tggatgtatc tacgccagcc
    88801 aaggaggtgc cgccacctgc gccgcaaccg cttccacccg cgcaactcag tggcgatttg
    88861 gtcagccctg acgactttga gtaccttggc gcgttccgcc tccccggtgg tgaagaccgc
    88921 ccgcagactt tcgcctacgg cggcaacgcc atgacctata accctgacgg gaactcgctc
    88981 ttcatcatgg ggcatgaccg tatgccctac ggcgacctgc cagatgggaa tcaagtagcg
    89041 gagatcagta tcccgaaacc ggtcatttcc aaaaacatcg aagacctcaa cacagcggat
    89101 ttatccaaaa attttcaaat gtgttgaaga gacaattcag cgagtacgac gaaatcccca
    89161 aggctggaat gcagtacctc agccgtgccg aaacgggagc caagattcat gttgcgtttg
    89221 gcgagcacct ccagtcggag caaatcccga cgcatggctg gttcagccct acgttgtcga
    89281 tccccgattt tcagggaaca tggttcattg ggaaccagga cctgtacagc gtgaacggct
    89341 acatgttcga aatccctgct gaatgggcag atacctgcac tggcggaaga ccccttggca
    89401 ctggtcgaat gcgcgacggt ggccagggtg gcatggggcc aacgctcttc gcttatcgcc
    89461 cgtggaatgc ggatggctct cccttgcctg ccgggacccg tctcgatgaa gttaccttgc
    89521 tcttgtatga gaacgcctac aacaccgagg aaatagtccg cagtatggac ggctaccagc
    89581 atccagatga atggggcggt ggcgcatggc tgacaacttc aggcggcaaa gcagcagttt
    89641 tatttgcagg gacaaaaagc aatggcgaaa aatactggta tgggtacatc caccccgatg
    89701 gcgcgaacct cgcctgtgtg gatgcggaag tcacggactt ccctacctgt cgtatggcgg
    89761 atggttcagt ctgcccgcag gaggattaca ctgcttgctg tgatgccgtt gcgggagaat
    89821 gtgtctccaa ccgtggttgg tggacaaccc gcttcgacgc cgagtttatc ctctatgacc
    89881 ccgctgattt ggctaaagtc gctaccggcc agctggaacc gtggcaacca caaccctacg
    89941 ccgttatcga cattgacgaa cacctctttc tgagtccgcc cgaatgggat ttggtcgagc
    90001 tcggctggag tgatcagcgc cgaagccgca ttggagatgt ggcatttgac cgtcaaaatg
    90061 ggttgcttta tgtgcttgag ttgtacggag atggcacaaa accagttgtg catctgtgga
    90121 aggttcatta gattaaatgg cgtatttacc ctgaagaggg aaattcaact ccaggttaaa
    90181 gaaactggtt gcagatttgc gattggacaa tgccatgccg ccaggcaggc tggggaagcc
    90241 ctgatgccct ggagtggaaa gcctgcgatg tgatctgcca gccacgatac ctaacgatat
    90301 aacaattaga tcccagatgg tgaggaactg cagcgaggaa gagtgtggtt caatgatggt
    90361 tcgttggtca ggtggaaata gttctttcaa gtcaagtagc attgaattat aataggccta
    90421 accgtaaaaa ttggtttcaa ctgaactgta tcaaaagcaa ttattttatg ttcattaatc
    90481 accttaataa cccaatattg acgccagcga caacgatata ctcccgtcta cacggtaaaa
    90541 agaaaaaaca taatggttgc agcaagctgg agaggtttaa ttttttggca cgatcttaaa
    90601 aatccgcacc cagcagattt aattgaagcg acattcgccc tccctgttcg gggttcgctg
    90661 atttttatcg ccaggttgaa aaccccgtaa tacagcgata tggacaccag cgaagcgctt
    90721 ttgctcttca cgaggattgg aagtgctttg gtggtaatgg aaaatcgcta aaggcaaggt
    90781 tttgaaaaaa aattcttttc ttatgtcaga ataacgtggc tcttaaacag attcgctcct
    90841 tttttcgcga ccccgagtac tatcgaggtt tgctgcgatt gatgctgccc attgcgctgc
    90901 agaacttgat cacatcctcg ctaaactttg tctccgtggt gatgatcggg cagctgggtg
    90961 aagtttccat cgcttctgtt ggattgggaa accaggtgtg gtttttgctc aatctgatgg
    91021 tttttggcat ttccagtggt tcagcgatgt tctctgctca actttgggga aaaaaggatg
    91081 tgcacaatat ccggcgagtg gtaggtctca ccgtcaaact ggggttggtc gctgccgcga
    91141 ttttctttat tcttgcacgt ttctttcctg gagccgcctt acgcgtttat agcaacgacc
    91201 cagaagtgat tgccactggc agccgatatt tacgcatcgt ttgctggacc tatggttttt
    91261 acgctctttc agctgtattc agtatggcct cgcgcgcggt agggaatgta cgtttaccag
    91321 tggcggtaag cacctcagct ctcattatca atgtgctcat cgcttatccg ctcatctttg
    91381 gcgtcagatt tttgggtatc cctgcgttgg gcgtagaggg agccgccatt gccggggtta
    91441 tcgcgagggt gatcgaatgt ggcgcgttac tgtttttcat ataccgtaat cgcgaaaacc
    91501 ccgtggcagc gggaccgcgc tacttgatgg agttggattg ggaattcatc aagcgcgtta
    91561 tggttccagt gctgccggtc attggcaatg aaatactatg gtcatttgga atcacgacgt
    91621 acaacgccat ttacgggcat attggtacca aagcggttgc agcgatcaat atcatttcca
    91681 cagtggacca ggtagccttt gtggtattcc tggggttggg aaacgctacc gcagtcttgg
    91741 tgggtaactt gatcgggcag gggagaatgg aaaaagcata cctttacgca ggcaggtctt
    91801 tgatactgca ggtgttaggg gcggttgtaa tgggattact ggtcatttta ttcgctccat
    91861 tgatccttgg tttttaccgt gttgacccag aggtgatagt gaacgcgcgc aggctattgt
    91921 ggatattggg cttgggcctg ggggtgcggg ctgccaatca tatgatagtg attggaatct
    91981 tgcgctcagg tggggatacg cgtttttcgt tagtactgga tggattggtg atttggctgg
    92041 tgggagttcc gtttacagct gcaggcgcgt ttctattcca tcttcctgtt tacctggttt
    92101 acgcgcttac gttgaccgaa gagattacca aagcctcgct tgggctgaaa cgttacttat
    92161 cgaaaaaatg gatcaatgat ctcacaggtc gtgtatcaga gatcatccct gagtgaaacc
    92221 tgctggttgt tcatatggtt ctggtttttt ggggtcattg accagcagta tcagtatgcc
    92281 gctggcaatg aatgccatca ccgccgaggt gataaacgct gtgacgtaat cgaacgcatc
    92341 cgccagaagg ccgccaatta atggggcgat aatcgccgct ggcgccacca gggtattggc
    92401 catgcccacg taggtgggtt tttccgcttc ttctccaaat tcaagcgaca tggtaatacc
    92461 aatcgtccaa aaagctgtat tcgctgcgcc gttgagtaaa aagaccagcg cgaagagatt
    92521 tacctcctta atcaatacgg caagtaaagc gctcaccgct gcgcatacgc tccctatcac
    92581 caacacccat tttcgcgacc atcggtcaga tagtcgtccc agtaacgggt tcattaccat
    92641 catggtgacc atcaaaatac tggtcatcac ccccacttga acagtggtca ttctaagaat
    92701 tttcactgca tagactgagt agaaggcaaa cgccatcata ccgaaatggg caataaaacg
    92761 gctgatcagg aagtgagtaa atgaacggtc gcgtttcagg atggaccgaa tgtcatgcca
    92821 aagaggttgg ggaacgcaac cgccggatgg catttctctt ttttcttccc tggtgaggtt
    92881 tagaaacacc caagaagaca caagtaaaag ggaagcaatg aaaaaacagg tggaaaagtc
    92941 gtagggaggg ttgagcacca ccagcagggt tcctgccagg tatgcaccca ggcttgccag
    93001 caggtttgct gctgccgatt gcgcaccaaa aaaagtcgcc aggatattgc tgggaatgat
    93061 tttgctgacc atgatttgcc aggcattagc cgtcagtccc gcgcccaacc cctgccatat
    93121 aagcaatatg aaggtaagaa tgagcgctac tcttacacca atgcgaggga caagcagccc
    93181 gacgattccc agccctaaaa tcggcaatct ttcctgaacg gtcataaagg tcacataggg
    93241 cttaacacgc tccatcctgg caatgcgttt cgccatcaac aattgtggaa gctgccagcc
    93301 gacgttgtga atggctggca cgagcccgat aagtgtggca gaagaggtga gcgttgccac
    93361 aaaaaggggg atcattgttg tgaacgaagc gaaacccaaa ccaaagccga acaaacctcc
    93421 atcgaggagg ttgacagtga agttgtgttt caagtttttg ctttgttgtg agtcgctcat
    93481 ttcaggcaag ttaaggtaaa ccccgggttg accggggttt gatttttcaa tcaacagaaa
    93541 agtgattaat cctcatcgtc ataatccaac tcatcccagt cgtcttcccc ttcctcttcc
    93601 atgtcttccc attcttccag ctcgtcttct tcggcgtccc aatcttcagt gcttaatgct
    93661 tctgcactga ctgtgtatgt tttacaacca gtatctgggt ccagttcaac cattccagcc
    93721 gtgcatatgc cttcatccag gaatgagcaa tcgaggtagt ggcatttgat tcttggcata
    93781 ttattttcct cctgatgctg tagtcgacgg cgattttact acaagtctga taattgtggt
    93841 gataaaaatc acactcatca tcatggctga caacaggcac cattgacaaa ccgttttcaa
    93901 cacaaaaatc tcgatgctgg tgagatacag ggaaaatagg aaccccgcca ggctgaatcc
    93961 aaacaggatc agatcgatgt aagggtgatt cgcgcgttgg tgtaaaccaa aagcagcgag
    94021 gaggaagatc aggccaaagc cggcaacgcc ataaaaacca agcgggatac caaacagtat
    94081 tgaccaacgg ctggcgttca ccacatcaca atgacccaat cctggcgtgc agtaaatggg
    94141 ggttttcgcg atctttacat atgcgaggta gatggagtcc agcaaaccga taatggcaga
    94201 aatctggatg atgagggtgc tcgttcttgg acgtgaagat ttcatttgtc aatcatctgt
    94261 tttcagagtg tcgggcattc tacaatgaat aggagattaa gtaaaggtta atcgccagca
    94321 cagggcacgg agaagagact attgaatgtg tttatccggg gttcaggcga tgcgcttcca
    94381 tgacattcgg caggttttca atgagggtga gaacgcgaga gagttgcacg atgtcagcta
    94441 tttccagttt cattacaatg gatgccaggt tgtgagagac attgatttta atgtccagca
    94501 aattgatatt ttcgttgctg agcaccgagg atatatcgac cattaatccc tggcggtcgt
    94561 aggctttaat ttcaatttcc accgggaatg tgtgctgcgg ttcaccccaa gccactcgca
    94621 caatgcgctc ccggtccgtc atgcgtaata tattgggaca atcttgacga tggatcgtgg
    94681 cgccgcgacc acgggtgatg taccccacaa tggaatcacc cggaacagga ttgcagcaac
    94741 gcgcgaaggt ggtcagaata ttgcgcaacc ccagcacaga cacggcgttc tcagaaatgt
    94801 ctttcgcctg ggtggatgac ttaatctcga acaggcgaga ctcgtcagcc tctccttctg
    94861 acagggtgtt gatgatatgc ccgagtgaca tatcgccaca gccaaccgcc acatacatgt
    94921 catctaaggt gcggtaatcg aaaccgcggg aaagtttttc taaatcatat tgttgcagtc
    94981 ccagccggcg cagttcccgt tccagtattt ctttcccctg ggcaaggttt tgttcacgat
    95041 tctggtgttt gaaccaggcg cggattttcg aacgcgcccg ttgcgttttt accaagccga
    95101 gggtggggtt caaccagtcg cgggaaggcc caccttgttt agccgccagt atttccacct
    95161 ggtcaccggt gtgcagcgtg taatccaggc tcacaagctt gccgttcact tttgcacccc
    95221 ggcaacggtt gccgatctcg gtgtggactg tataagcgaa gtcaatagga gtagcgcctg
    95281 cggggaggtc aataatatcg ccgcgcggag tgaaaacata aacccggtcc tggaaaacat
    95341 cggttctcat gccttccacg aactcattcg cgtcttccac ttcctggcgc cattccatca
    95401 actttttcaa ccagttgata cgctgctcat attgctcatt ccgcgggcct ttttctttat
    95461 aacgccaatg cgccgcgatt ccgtactcag cgttttcgtg catctcaggg gtacggatct
    95521 gtacctcgag cggctttcca tcgtcataca gaaccgcagt gtgcagggac tgataaaaat
    95581 tatcttttgg cgcggcaatg taatcatcaa attcgttggg gactggccgc cagtgggtat
    95641 gaatgatacc cagaaccgca tagcaagagg gaatatccgg aacaatcagc ctgaccccgc
    95701 gcaggtcgcg caccagttca aagggtttgt ctttctcaac catcttccgg tagatcgaat
    95761 agatatgctt gctgcgccct gatatttcca ccttgatgcc agcctcattg atcaattttg
    95821 ccattcggtc gatgatggct ttcatctggc gctcacgctc taacctcgtt tcagccaggt
    95881 tctgggcgat ttctttatac ttctccggat tggtataacg gaaccccagg tcttcgagct
    95941 cccatttcaa ttgccagata cccagacggt tcgcgagcgg ggcgaagata tccagtgttt
    96001 cctgtgcgat acggcggcgt ttagcttcag gcacaaagcc gagagtgcgc atattgtgca
    96061 ggcggtcagc cagtttaatc aagataacgc ggatatcttc acccattgcc aagaaggtct
    96121 tacgcagcgt ctcattggca aggtcacggc ggcggtcgcg ggcatgctgc gagttcaaca
    96181 gcatttcctg ttcttcctgg ttcttttcca tgttcttgcc aaaaatttca gcgtcgtgct
    96241 ggtcggcccg tgatactcgc ggcaggtggg tcaacttcgt caccccatcc acgagcttgg
    96301 ctacctcatc gccgaagtta cgttgaatgt cttgaagcgt gacgctggta tcttccacgg
    96361 tatcatgcag caacccagca gccaccacta ctggctgaac gtgcatttca gccaggatga
    96421 tggaaaccgc cacgcaatgc gtgatatagg gctctccaga agctcgtttt tgttcggcgt
    96481 gcgcattttc cgcaaagttg tacgcctgaa tgatgaactc tctatctact ctggaataat
    96541 tgtccggcag aatatctaaa aggttttcta gtaaggggct ggttttcgtt gtactgacca
    96601 tgttcgactc ttgttaaact cttagtatat cacccgacaa gaaaaaccag accttttccc
    96661 ttctttgttg tgcagaacgg agatccacgc atacccatac gcccgagtgt ggcgtactct
    96721 tccaactggg ttaattcata gaaagggcaa gttactttaa tagttcctgg ccttcaaatt
    96781 ctcgttgtgg agaaagatag cgcagtgtat cagcgtcttc cgggagtaca tcgccggtaa
    96841 cagcgcgggt tagcagttcg ccgagggctg ggcctaacat aaagccctga ccgcacaacc
    96901 ccactccgag aagcaggttg ctcacctgcg agctccagcc gacgatcggg aacccatcag
    96961 gcgtcattgg atagagaccc cgccatgtcc ggcgcacgcg gatgtttttc agcctgggca
    97021 tgatggaaat catccgcctg gcaaccattg gcagaaagct actggtttcg ttcacatctt
    97081 ctccccagat atttgggctg ggagtgatgc agaaaatcac ctgaccggtg gtgtgctggt
    97141 aaaaatagta atttgatgaa ccaggtgccg gccgaatgtc aacgatcata gggccgagga
    97201 acctcgccac cggctctgtg atggctgcct cgtggctgtc ggcacgaact ggaatttcaa
    97261 cacccgccat gcgtccgatt tcggcagcgg aagcgccggc tgtgttcagc acgacaccgg
    97321 ctgaataggt ttcacgactg gtggtgactg aaataacttt cccccgcttc acaaccatgc
    97381 cggtgaccgg ttcacaaaaa tggaatgtaa ctcccttctc tacggcgagg gtgaaataag
    97441 cgtgattgga taagagcggc gaggcgctgc cgtcgttggg ggagaaggtg ccgccaagca
    97501 ggccctgagg agtcaggtca gggatgatct ttaacaggtc atctttcgcg taccagttgt
    97561 tcaccaatcc atacgtatgc tggatggtca gcaagtcttt gagaatcttc tcttcacggg
    97621 gagtgtaggc aatgaaagag tacccacact gatcccactc gatatcttta ccgtaaactt
    97681 ctttgaaggt ggaaaagatc tcaatgctgc gcaggcaaag acggatctta gccggatcgg
    97741 aatgggtggc gcgtataccg ccgatggcgc gtttgtttga tccctggcca acgctgggga
    97801 ccgtttcaag cacaagtact tttaaaccag cagaagcgaa ataaaaagcc gccggggttc
    97861 ccagtgaacc cgcgccgata atgatgacgt cgtatgtgtt ttgggggttc attggttatc
    97921 accctccccg ttttcgcccg cgaagacgcc caaacctact tccacaaaca acggacggcg
    97981 ggagggatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    98041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct tccacctgaa
    98101 tcacaatggt tctgtcgttc gctttcaagg cgcgaatgtt caccacttca gccgtaccaa
    98161 gttcatctcc ctgggtgtct tccagggtaa cctggtcgcc gatatggatg gactcgcggg
    98221 agaactcata agggatggac actaaaggtc tttcgctact gctgcggtaa tctaccaggg
    98281 tgatcgccag acccgggcag ccggcaatac attttaaaca ggctttacag gcgaagttat
    98341 cctggacaaa ttttggcgtt gaacggatat cctgctggtc gataaagatc aagtgttgag
    98401 ggcacaaagt tgaacaaggg tcgcagggaa tttcttggct acagtggaaa accggcagca
    98461 cgccttccgg caggtcgggc attttttcac tgagcacatt tcctggttta gatttgagga
    98521 tttcgcccat gcggtaccat tctgcaggga tttcaccagt gtttagaccc aaatgatgag
    98581 ccacttcaag ccctttgatc ttgccgctaa aaatggccgc ggatgcttct gcaatctctt
    98641 cagcatctcc ggcgacaaag gcagggatac caaagtcatt ggctttgtgg aagaattcat
    98701 tcactgggtc aagaccgacc gcgatgagca cagaatcgca agcgtacgac ctttcactcc
    98761 ctggtactgg ttggaattgc tcatcgaccc tggcgatggt gaccgattca acatggtctt
    98821 gtccgttggc tgagaggatg gtgtgcgagg tgtaaattgg cacgcccatt cgcaccagtt
    98881 tatctttatg cactttataa ccaccacatt gtggaagggc ttcaaccaat ccgacaacgc
    98941 caatcccggc ctgaagcgcg tgataccctg caatcaagcc gacgtttcca ccacccacga
    99001 tgaacagctt ttctgccggc aaggcaaggt cgcggttgac cagcgtttga aatgcaccgg
    99061 cgccgaagac ccctggcagg gtgttgcctt taaacgcgag gaatttttca cgtgcgccgg
    99121 tcgccaccag caacacttga gggcggataa gaacatagtg cttgccatct tttaacactc
    99181 ctaccttctg atcgctgaaa acagccaaac aagtggtttg cggccaaatt tgaacagaag
    99241 ggtacgcacg cacttcgttt tccaacctgg tggcgatgtc tattccacgg gtgccggcgt
    99301 aaaccgcttt ggaggagcca aaaaagcggt gggtttgcag caccaactta ccaccaagac
    99361 ggtgcttatc atccaccagt ataacttctg cgcccagctt ccccaattca atggctgcgg
    99421 atagaccggc agggccgcca ccgataatca gcaccggaat gtcgatttct gggattggct
    99481 ttaattcagg tactacgctt acttcaggca gacgagggtg accgtcgttt ggataaaccc
    99541 gcatcccgtt ggtgacaatc tccatacagg attttaaagg tttgccatcc gcgattacca
    99601 tacattgcgc gcactgaccg ttggcgcaaa aaattcccaa aggtgaacca tctttgtgat
    99661 gatgcccgaa agtgcgaata ccgttggcga aaagcgcggc ggagatggtt tcacccttat
    99721 gagcggacag gggtttgtcc tgccagaaga actcgatcat ttcaggttca gggatgggca
    99781 agatgggatg ctgcttgata cggtaaccgg tcatggatta tcctcagtat acggtttggg
    99841 gtttctaccc gtagaacgag atgggaactc aagcgttttt aattttacca aatagtagaa
    99901 aacaggcgca tcggtttttt cgggtatttg taacctatga ttattggagg aggaaaagcc
    99961 gggtgcacgc cacctggtat tgcgcgccct gcaggtcctg aaggctatcg gagcgtaaac
   100021 ctgcacaaac cgagtgagaa ggaaagcaag agggtgttgt gttcaaccag tgtctgcaat
   100081 ataatgaatt tcagataata tcgaacaatt gaaccttgga gttctaggga gagacccatg
   100141 aaacgcattc tgttttcgct tatcgcggca ctcttcctgc tcggggcagc gccgcagccc
   100201 gctccgacga tgcaggctga caaccccaat ccgccaactc agacggtaaa actgattttc
   100261 atccatcact ccactggaga gaactggctg cgggacgaca acggcggcct gggcatggcc
   100321 ctggctcaga acaactactt tgtcagcgac accaactatg gctggggacc ggattccatc
   100381 ggcgacagga ccgacatccc taattggacg gagtggtttg ccagcgaaaa cacgccgacc
   100441 atcatgcagg cggtctacaa cgaaagtagg cagaactcgg agtatactcg caaccggcct
   100501 gaccccggag gggagaatac tgtcatcctt ttcaaatcgt gcttccctaa ctcggcgctg
   100561 gagggcaacc cgaacgatcc tccctctgag gagggtgacc tgaccgtggg gcatgccaag
   100621 tgggtctaca accagatctt gccctacttt aaaagccagc ctgacaagct gttcatcgtc
   100681 atcactgccc ctccgctatc ggataatacc tacgctcaaa atgcccgcga cttcaataac
   100741 tggctggtga acgactggct gcgagaaaac aactacacgc tcaacaatgt agcggtctat
   100801 gacttttaca atgtgctcag cgcgccggac aatcaccact ggtataacaa cggccgggtg
   100861 gaggacgtta tcaacaaaca gcgcaacacc aacgcctacc cctcggggga tgatcacccc
   100921 aaccaacggg gtaaccgcaa agccagtgaa gagttcgtgc cggtgttaaa cgtgttttac
   100981 aaccgctggc gggcatccgc tcccgcccag cctccggtac aggaggaggt ccaggcacat
   101041 actggtcagg atgggcaatc tccgcttcag ccaggcagcc agccggaagg cggcgggttg
   101101 atcgacgcct ttgaaggcgg cacgccccct ggaacgaccg gctgggagac ctaccgagac
   101161 gaaaccacgc caagtattat cacctgtgcc cccgacagca gtatggcgca tagcggaaac
   101221 gcttcgatgg agatggaatt tttcgtaccg gcagacagtt gggtaacctg tatgctttac
   101281 aatgagcaac ctcagaactg gagtggacaa caggggatta gctttttcat ccacgctcag
   101341 caggcaggca cagtcatcca cgtcaacctc tacgcgggcg acccggctaa ccaggaatca
   101401 cacgtttacg tgctggaaac accgcctgat agcggcagcg gctgggcttt gatcgagatc
   101461 ccctggggtg cgttccatcg tgtggagtgg gaggagaata gtggcgtccc gttcaccctc
   101521 accaatgccg tcctggcgat cggttttggc gcgtccgcca atccagatga gaataacaag
   101581 ggcaccgttt ggattgacga cctgggcttt ttgggcagcc aggttggcgg ccagccgacg
   101641 accgccgccg agaa
//
LOCUS       DAOSPM010000002        75421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_356_length_75421_cov_15.649842, whole genome shotgun sequence.
ACCESSION   DAOSPM010000002 DAOSPM010000000
VERSION     DAOSPM010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 75421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75421
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_356_length_75421_cov_15.649842"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..507)
                     /locus_tag="PLW25_00400"
     tRNA            complement(435..507)
                     /locus_tag="PLW25_00400"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(472..474),aa:Ile,seq:cat)
     gene            707..1192
                     /locus_tag="PLW25_00405"
     CDS             707..1192
                     /locus_tag="PLW25_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOS52977.1"
                     /translation="MSYPSTSGRDLVILEQIEQDPDTTQAALASSLEVAVGTVNWHLK
                     RLIEKGYVKVSRLERKKLKYIITPEGIALRARLTVDFIQNSFNLYRLVRERAAHAIQN
                     LKALGYHQVRLEGSGEVAEICRLTCLEHEITLVENESAPVLKLVGLKVILETKEHEHV
                     T"
     gene            1176..2129
                     /locus_tag="PLW25_00410"
     CDS             1176..2129
                     /locus_tag="PLW25_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOS52978.1"
                     /translation="MSMSPDYWQDKRVCVTGGAGFLGSFVTARLHERGAKEVFIPTIE
                     KYDLVQLDSIRQMLDDSNPDIIIHLAAQVGGIGANREHPAEFFYSNLMMGVQLIHEAW
                     LRGVEKFVALGTICAYPKFTPIPFKEENLWDGYPEETNAPYGLAKKMLLVQSQAYRQQ
                     YGFNSIFIMPVNLYGPRDNFKPESSHVIPALIKKCVEAVESGADFIEVWGDGTPTREF
                     LYVEDAAEGILLATEKYDSPEPINLGSGMEISIKHLVELIAHLTGFKGRIIWDTSKPN
                     GQPRRRLDVTRAEQAFGFKAGTSFEEGLEKTIEWYRGQKGY"
     gene            2138..2995
                     /locus_tag="PLW25_00415"
     CDS             2138..2995
                     /locus_tag="PLW25_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS52979.1"
                     /translation="MATVNTLEQKRKPPVVVIQPSRGWVGLKLKDLWLYRELVYFLTW
                     RDIKVRYKQSVLGILWAILKPFMAMVVFTIFFGNFAKIPSDGVPYPIFSYTATLPWEL
                     FAAALGVASRSMVSNSNMVSKIYFPRMIIPLASVMSSVVDFFIGFTILIGMMIFYKVT
                     PTIATLWLPLLILLALITALGVGFWSSALMVRYRDVGYIMPFIANLWMYLTPVVYPSS
                     MIPEKWRLLYALNPMTGVVEGFRYALLGTTQSVSGGMILVSSLIAIAVLISGMFYFRR
                     MEKQFADMI"
     gene            3007..4272
                     /locus_tag="PLW25_00420"
     CDS             3007..4272
                     /locus_tag="PLW25_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS52980.1"
                     /translation="MSNLAVRVEDIGKQYNIFQETARYITLREKIVETLKTPVKTLLN
                     PTGSEKFWALRHVDFTLDHGQVLGVIGKNGAGKSTLLKILSRVVEPTEGLAEIHGRVG
                     SLLEVGTGFHPELTGRENIFLNGAILGMKKSEIERKFDEIVAFSEIEKFIDTPVKRYS
                     SGMYMRLAFAVSAHLEPEILVVDEVLAVGDAEFQRKCLGKMSDVASEGRTVIFVSHNM
                     SAIQRLTQECIILDKGAMVLRARTPEAVDYYLTNDFSASGERRYTEEESSSAAYPFKP
                     VAVRVKNPSGQVTDSLFSVDPLTIEVEYQLDADITGLRIGIYMMTSRGEFIFTSFDTD
                     EPEKFQSMSMRKAGHYISRCVIPADTFNEGRFVIGINASSYMVKRYFSDERAMNFSIN
                     TTGAPGMHWVEIRHGPIRPRLDWRIEEIA"
     gene            4273..5853
                     /locus_tag="PLW25_00425"
     CDS             4273..5853
                     /locus_tag="PLW25_00425"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52981.1"
                     /translation="MASNKELLKKILGELPLTAEVYQLLQQSDRPLPNHFSLRNIRKV
                     LPEAVAAVQKSCAQYPAGKNVFIFASTHYWLEHGVITGLALSGLGHDVTIGYYPYGRW
                     DFNSPQFDVRRQSVYSATVLRPASAVMNFENLLHTHSSFSLLPEALQQAVDQVTDFDY
                     MYAFQTEEVNREDPFYLFRHERNLHAAKSLLTYLRSLKPEVAIIPNGTILEFGIAYKV
                     CGYLKIPTTTYEFSDKRHSMWLAHNQEIMQQNTDELWKTHANYQLTNADLEELKVLLS
                     SRKNASTYGDFSRQWQRVPAEGQAAIQAKLGFDGKPIVLLATNVLGDSLTLGRNTFSR
                     SMSEWIIRTIQYFIEKPEVQLVIRIHPGEALIKHGSILDLIRKTLPTLPPHIHLIEPL
                     AEVNTYDLMDIADLGLVYTTTVGLEMAMRGIPVIVAGKTHYRNRGFTHDPNTWVDYFK
                     LLNRLLTCLRECRLQEDQVNLAWKYAYLFFFKYPLPFPWHMTRLKEDLEEYPLEYVLS
                     EPGLEKFRSTLQLLLSTR"
     gene            5862..7370
                     /locus_tag="PLW25_00430"
     CDS             5862..7370
                     /locus_tag="PLW25_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52982.1"
                     /translation="MMDDQEYENLLFRRQYFIGPKELGISGWNLTHLPDGKILSAHPE
                     LEVTDWNGKHTRYVLIGYIFDPFHPERTNQGILEAIDRCKTDTGSIFEMLEDKSGHYV
                     LFVYDDEMNIALTDACGIKQTSYYCDENGKSYYSSQPALIAQKLNLPVSDEAIHYMDS
                     AKYKKKLEPWWPVESSPYKRIRRLVPNHYLNLATNEQVRFWPNKKLKRYSLKEGTILA
                     GELLKGICKACFQRYPMALTFTGGYDSRVLLAACKEFADQIELFSMIYRNLTEQSEDL
                     RIPQAIAAEKQLKYHQIKCDAEIPAGFQDVYERSNQGYKTDWMSLVYGRYIHFPANVL
                     IVKGNIAEVARCSFWQDGIYPVEVTVDTLVDATALGHEPLILQSMGKWLDEAKPTEKF
                     GYKIMDIFAWEVEAGGWQSMSHNVFALSQEEFSPFGNRRLLDIMLGVHKRYRCWPDIT
                     LEQEIIKYLWRDLSQYSYFSSWNLHNYKKKFYDGDLLNFLRKIKLILKRSIK"
     gene            7367..8374
                     /gene="pseB"
                     /locus_tag="PLW25_00435"
     CDS             7367..8374
                     /gene="pseB"
                     /locus_tag="PLW25_00435"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HOS52983.1"
                     /translation="MNWSDSVVLVTGGTGSFGKKFIDIMLKEYHPRRLVVFSRDELKQ
                     FEMQAAGFSHPSLRYFIGDIRDLQRCRRAFEGVDLVVHAAALKQVPACEYNPMEAIKT
                     NILGSSNVIDAAIDAGVSRVVALSTDKAVNPINLYGATKLAAEKLFVQSNAYAGARKT
                     RFACVRYGNVVGSRGSVVPFFLKQRETGRVTVTDERMTRFWISLEQGVRFVIRAVENM
                     HGGEVFVPKIPSMTVTDLARAIAPEAAIDFIGIRPGEKLHEVLISEDEARQTVELEDM
                     FVVQPAESLWFGRDWEGKGRMLEDGFRYSSNNNDQWLNVEQIQSIIAPIEADYLAGRL
                     G"
     gene            8371..9291
                     /locus_tag="PLW25_00440"
     CDS             8371..9291
                     /locus_tag="PLW25_00440"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS52984.1"
                     /translation="MTRFLVTGASGLLGLNFALAVDGKKHQVTGVANTAPMLWATFKN
                     VQLELTQPGVVDEMIKEHQPEVVIHCAAMANVDACENNPAQARLVNAELPGTIAAACK
                     RHRVRLVHISTDAVFDGKKGDYREEEPPHPLSVYASTKLEGERAVLAANPDALVARVN
                     FYGWSAAGNRSLAENFVRNLEAGKEMMGFTDVIFCPMNILDLSSVLVEAAELDIRGVY
                     HMVGAQAMSKFQFGVVLAEQFGFDTRLIKPVSVNDSGLKAARSPNLSLSTEKIVKALG
                     HELPDFHEGVKKFFDQYRRGYPQYIMSLNG"
     gene            9314..10381
                     /locus_tag="PLW25_00445"
     CDS             9314..10381
                     /locus_tag="PLW25_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOS52985.1"
                     /translation="MELKIGQHFIGLQHPTYFIADISANHDGSLERAKMLIRLAKEAG
                     ADAAKFQNFRAPKIVSDYGFKAMQGQVSHQATWKKSVFEVYSDASIPFDWTPILKEEC
                     DKLNIDYFSSPYDFEAVDYLDPFVPAHKIGSGDIDWLEMLEHIALKGKPVILSSGAAT
                     IGEVQQAVHTILPLNQQLVLLQCNTNYTASPVNFDHIHLRVLQTYQTLFPEIPVGLSD
                     HTHGHATVLGAVALGARVIEKHFTDDTSRVGPDHPFSMTPVTWREMVDRTRELERALG
                     SADKSVSGNEVDTTIVQRRCLRASRDIRSGEVFTREMIDVLRPATPGAIRPDQVFQVV
                     GTRALKDIPMGKELRWTDLGE"
     gene            10383..11510
                     /locus_tag="PLW25_00450"
     CDS             10383..11510
                     /locus_tag="PLW25_00450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS52986.1"
                     /translation="MKVVYFTRDYSPHDERFLTALAQTQHEVYFLRLSPSSEVHLPPG
                     IREVKWVGPLAGEIISDDRRAEDLNQLLSRLQPDVLHAGPLHGPAYIAGLSGFTPLVS
                     MSWGSDILHDGEVDALARAKINLSLHRSTVFLGDCQAVVEKAINAFAFPAERVFRFPW
                     GVDLAHFSPSTTSPLRASLGWQQQFVFLSNRSFEEIYGVDVVMRAFIQAQSRAPNIRL
                     LLYGKGSLQQEIMRMAENAGVMHKIHFAAFAGHEDLPASYHAADVFLSASHCDGSSVS
                     LMEALACGKPALVSNIPGNLEWVHHGENGWLFQDGDTTGLADAMVAVSRKPNLEKLGQ
                     KSRALAEEKADWSCNFAVLLQAYQKAVEWRTPALEITERVK"
     gene            11507..12262
                     /locus_tag="PLW25_00455"
     CDS             11507..12262
                     /locus_tag="PLW25_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOS52987.1"
                     /translation="MKVVAIIQARMSSTRLPGKVLKLASGRTMLDRMLERVKKARTIS
                     EVVTATTTDPSDDSIARACKKLGVSVFRGSLQDVLDRFYQASRMHQADIIVRLTGDCP
                     LIDPALIDDVVNALTRKKVDFACNRLPPPFSRTYPIGLDVEACTFNALEQAWYGAKEK
                     FEREHVLPYLYAVPGRFSVLQLNHTTDLGHLRWTLDTPEDLVLLRRIYRYFQGRNDFS
                     WLQVLQLQQQKPSLFAINAQVRHKTYLENESPR"
     gene            12259..13122
                     /locus_tag="PLW25_00460"
     CDS             12259..13122
                     /locus_tag="PLW25_00460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS52988.1"
                     /translation="MTIKEMSEKIVCPRCQSSFDKHDPAVWSCSSCAYTVPLFQGKPV
                     FSGHPQEQQPQDKIERGADKGTAWRRANWRFIHAIAASLPAGADVLDVGAGRGDFRQI
                     FAHHSYIGLDIYPYPEIDLAVDLIEINPFKERSFDLVVLANVIEHVYEYRKLISRCAF
                     LLKPGGQLLITVPFLLKLHQEPVDYHRYTHYALFQLAEENQLSVARFEVYTNPFALLD
                     EGIADVWENVLPLRQRIDHLHTKVKVALIQKLANSMKANQADGVVKPVQENSNPRVLG
                     YMCLFQKDALQ"
     gene            13119..14051
                     /locus_tag="PLW25_00465"
     CDS             13119..14051
                     /locus_tag="PLW25_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52989.1"
                     /translation="MTSVAFFTAPKPFTNAHIRIIQRNAIQSWKSLGRQVEVWLVGDE
                     PGVEQNARELQVGYIPGVARNSFGTPRIDSIFHLVREQSQAEYLCYVNADILVFPDML
                     RALEEVRARVDRFLIIGRRWDAAVTETLDIKEGWHQQFQAAALVTAKLHRAAGSDYFL
                     FPRNEFIDIPGFAVGRAGWDNWMIYKARYEHMAVVDATGAIKVIHQNHDFSHFADGKI
                     HRLQPESAENMRLAGGRYTVFTIHDANYQLDNDGLQRVRLNRWKLLREFVIFPAVTLH
                     CSWLAKVVYAIFNPKRLLKDRRQAKEALRRSKEG"
     gene            14052..15008
                     /locus_tag="PLW25_00470"
     CDS             14052..15008
                     /locus_tag="PLW25_00470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS52990.1"
                     /translation="MRKGQNPVKQLQTVSKPQRVTVAVLNHIPFLDGFYAQMLDVLQL
                     SLSTLRQNAGLDFDLLVYDNGSCKQARDFLLAEYEKGNIQSLILSDRNMGKGGAWNVM
                     LQAAPGEIIAYADNDVFYHPGWLRESVKVLETYPNVGMVTSRPFHTKPELYSADLDWA
                     RKTPEASVERGFFIDRDWMREFWLSVGRSEEEIALDQAVEDVRLTYKGLVTYCGASHW
                     QFLAWKKVLQEFLPIDLSRPLGQVLKLDEMINQQGYLRLMTAQPFTMNLSNSVELPSN
                     EPTSAAAQPRTRKRLADFPVVKSPLMRLYNAIFKLYYDDKKR"
     gene            15019..16176
                     /locus_tag="PLW25_00475"
     CDS             15019..16176
                     /locus_tag="PLW25_00475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS52991.1"
                     /translation="MKILYATGRYSPLKHDDGSGTDYRLYHALLSQGVEMKFIGPFPD
                     HPSLIERLYRKVHTVFSHKRPAKYTAAMLKTAAQTLSITVNEYQPDVIFSKNLAPLVR
                     FHAQLPVVYMLDSTVAGFNRGWPTFSRLENWRMVEWEKKVLKQSSSVITRSEWTRQSL
                     IEDYHYPAERIHIVHNGSSLPDHVIPQEIENEPPDFSTIRLLFVGRVMHLKGIDIAIE
                     IVNQLNECGRQADLRIVGLDGQDQDHIHYMGSFKKSIEDQLIAYANQYRWPHFLLHPA
                     RYDAAPIVTAEAAAFGVPTLTNNVGGIASMVQDGLSGVVLPALSPAAEYVKKIVYYAD
                     HPQEYMALRRTTRERYEKELNWNVAGKKIVTILQSVVEENQLKPGLKKEQP"
     gene            16173..17156
                     /locus_tag="PLW25_00480"
     CDS             16173..17156
                     /locus_tag="PLW25_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS52992.1"
                     /translation="MKILIAGYGSIGRRHLNNLRALGQTDLLLLRSHRSTLSDVDIQD
                     VPVETTIEAALAHQPRAVVIANPTALHLDVAIPAAQAGCSILMEKPVSHSMSRVEELR
                     RALTSGGGVLLSAFQYRFHPGLRQVKRWLQEDQIGVISSVKSHWGEYLPGWHPWEDYR
                     SSYSARVDLGGGVVNTLCHPFDYLRWMFGEAEQLVASTSSRGLNLPVEDTADVLLRYQ
                     GGVCANIHLDYLQRPGQHDLLITGSAGTIRWSNTDGSAKRYDPIHDQWEEVRPPEGFE
                     RNTLFLEEMAHFLRVVERQEAPLCTLEDGIAVLQLTEAVHRSAREGCLVKF"
     gene            17175..>17668
                     /locus_tag="PLW25_00485"
     CDS             17175..>17668
                     /locus_tag="PLW25_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS52993.1"
                     /translation="MTSIFEKFSLKGRVALVTGGAGLLGKEFCLTLAQAGAQVYVADL
                     NLSAAESAAQTITAQGHSACGIRLDVTNPDSVQSVVDEIVSRSGSIDVLVNSAAMDPK
                     FDPGHAQTNSNAFETYPLGSWQDALNVNLTGPFLVSQAVCRQMVRQGKGSIIHICSTY
                     GLGGP"
     assembly_gap    17669..17768
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17864..19120
                     /locus_tag="PLW25_00490"
     CDS             17864..19120
                     /locus_tag="PLW25_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HOS52994.1"
                     /translation="MVKKPEVLALIPARGGSKGIPRKNIKLFAGFPLISYSIAAGRQA
                     QSVTRVIVSTDDEEIAAEARRWEAETPFLRPPELAQDQTTDLPVFQHALRWLRENEGY
                     TPDLVIQLRPTSPIRPRLCVDEAVALLRNHPGADSLRGVVPSGQNPYKMWRLQGKDEP
                     MTPLLKLDGIAEPFNAPRQSLPPTYWQTGHIDVIRPRVILEGNSMSGRNILPYLIDPR
                     YTVDIDTPFDWLRYEWLVRNADLDMVDPASKRRKLPAKISLVAMDFDGVLTDNLVYVD
                     QDGSERVASSRGDSMGIRLVREALPVNFVVISTETNPVVSARCRKLNLEVYQGVNDKP
                     AVLKQLLQERQVPAGEVVFIGNDVNDVGCFDLAAYAVAPADAEPEVRRRADLVLTRKG
                     GKGAVREFCDLLLQNAGKILPTQEKK"
     gene            19117..19992
                     /locus_tag="PLW25_00495"
     CDS             19117..19992
                     /locus_tag="PLW25_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOS52995.1"
                     /translation="MTREVKIGNKWVGDGHPVYIVAEVGINHNGDIEIAKELIRAAKH
                     AGVDAVKFQKRTPELCVPPEQRGQMRETPWGYISYLDYRYKVEFNEADYDEINRYARE
                     IGIEWFASVWDIPSVDFLEKYEPICYKIPSASLTDVELLLHLRKTSRPLILSTGMSTQ
                     QQIKDAVKRIGEENLVITHATSAYPCDPYELNLRMIQTLRGIFNCPIGYSGHEVGLVT
                     SAVAVGLGACMVERHITLDRALWGSDQAASVEPGGFEKLVKYIRVTEAAIGDGVKKVY
                     ESEMPSLRKLRRIQD"
     gene            19989..21599
                     /locus_tag="PLW25_00500"
     CDS             19989..21599
                     /locus_tag="PLW25_00500"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52996.1"
                     /translation="MNKAFLRVRNGIRQRWVHKKANQRWRALATAITLQSPLPSDPRP
                     VIFFNASTRLQAMSQNAAYALLTAQALRLQGVPVIQFVCGGALQRCVLGSQQDNFNLP
                     PPCQACTRQSQAVFQELNTRWMETREHPMLGKELETLSFNEYETFTYQGTPLGAWAVN
                     SLRWMMRRHHLQEDETTRAFFRSFILSSWNVFTQFSELLDETQPRAVVLFNGMFFPEA
                     AARHACLERGVRVITHEVGLRPFTAFFTTGEATAYPMIIDPAFQLDDQMEKKLDRYLS
                     TRFRGEFTMAGIRFWPEMKALEADLLEKINHYSKLVAVFTNVIFDTSQVHANTLFEHM
                     FTWLELVKTTALSHPEVLFVIRAHPDECRQGKESRESVATWFSSSEISRLDNVVFIGA
                     DEYLNSYDLIRRAHLVMVYNSTIGLEAALLGKPVLAGGKARFTQLETAFYPQSAQEYT
                     VMLERLLATPSIDTPPEFARNARRFLYYQLYATSLDFSDYLEEDGVWKGYVKLREFSP
                     DLLKPDNSLTMKVLLNGILENGDFTLPL"
     gene            21596..22540
                     /locus_tag="PLW25_00505"
     CDS             21596..22540
                     /locus_tag="PLW25_00505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS52997.1"
                     /translation="MTTPVCSIIIRAYNEEKHIGRLLRGIQEQDLHDVQVILVDSGST
                     DGTRDVARAYPVEIVEIDPREFTFGRSLNLGIAAASAPLIVMASAHVYPVYPDWLTKM
                     LQPFQHTEVGLVYGKQRGTGTTAFSEHQIFSHWFPDVSVKKQSHPFCNNANAAIRKEL
                     WQQHPYDETLPGLEDLEWGKWAQEQGYTISYVAEAEIIHVHNESLDGIYNRYKREAMA
                     FKRIYPHETFTLRDLNRLFLQNVISDWREARRQKVFAHHFFHIIGFRWRQFYGTYQGY
                     RQSGPLTWQLKRAFYYPRDFRQASGQTSRRDIEPIKYN"
     gene            22575..22862
                     /locus_tag="PLW25_00510"
     CDS             22575..22862
                     /locus_tag="PLW25_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS52998.1"
                     /translation="MAHVTIEFTGLSKSITGVNEVNLPINPTDTYRDVVRQLGTLYPA
                     LVGILIAEDGETFLSSNMFIIDGDYANPAMVMENSPRDGEHIHLMSVITGG"
     gene            22888..23568
                     /locus_tag="PLW25_00515"
     CDS             22888..23568
                     /locus_tag="PLW25_00515"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HOS52999.1"
                     /translation="MPEKLQHPKISALVPMRHHSQRVPGKNYRPLNGIPLYQHILTTL
                     TQVPEVDEIVVDTDSDIILHGIQSNFPAVKVINRPPELRADDISMNTILLHDISQSPA
                     DLYLQTHSTNPLLKASTISRAINKLLQVYPEHDSLFSVTRLQTRLYDHLGKAINHDPK
                     VLIQTQDLPPVYEENSCIYLFTAATLRKNGTRIGETPLLFETDPEESWDIDEEVDFKL
                     VEMILKAR"
     gene            23572..>24197
                     /locus_tag="PLW25_00520"
     CDS             23572..>24197
                     /locus_tag="PLW25_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53000.1"
                     /translation="MKRFSSFSVLLQLGIFLLGVSGSIYVAFTPANSMMNWYSNDDAF
                     YYYKVAQNVLSGHGFSFDGINLTNGFHPLWMVICLGVFWLSRFHLLLPLRVLIVISGV
                     LNGLTGVVLLRLLQKFLPLVAAILGACVWILLPSIYNNYTAHGLESALSAFFVAVLLL
                     KSADLLTGPAEKRAGKLIILGIIAALTILSRLDTLFVVAMVGFFVVFK"
     assembly_gap    24198..24297
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24298..25385
                     /locus_tag="PLW25_00525"
     CDS             <24298..25385
                     /locus_tag="PLW25_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53001.1"
                     /translation="FYRPSSQVLSVSAWQQVKNWIRQGLPHHLRDGVLFSFPIVLLVC
                     GYMLTNKLEFGTFIPVSGQVKTWWGTLENTVYRQNTTLVGLMGLAPGRGKSPWSLLTS
                     IVADISIFIRNLFVKDTEDLPVILFLVFLFMLFILLIFLLSRKDGLLARRSFALLIPA
                     LAGGCLLHIAYYAARNYGHTRSWYWVPETMLLVLLGAVVSSRMFEKIRQWTRTPVVEN
                     LLLLVMVGFVIFLHARYIARLCPPSVSPEHQADYLAETRELESYTRENSLIGMTGGGT
                     TAYFIQHRTIINLDGLINSAEYFNTLKTGTTNRFLNNLHLDYVFGNSYMLLETDPYQQ
                     IFTGRIREIGNIHSGSHFTLYDYIGDQ"
     gene            25457..26428
                     /locus_tag="PLW25_00530"
     CDS             25457..26428
                     /locus_tag="PLW25_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOS53002.1"
                     /translation="MPVLLLSAPYMLPFVNRFKPVFDHYQLELLLPQVNERLDEEALL
                     SLAGQFDGIICGDDRFTPRVLEACSPRLKVISKWGTGTDSIDRAACERLGIQLKNTPN
                     AFTIPVADSVLGYILAFARRQPWMDRAMKSGKWQKIPGRALSETTLGVVGVGNIGKAV
                     LKRASAFGMRLLGNDIIPIPADFLQEYHVESVSLPELVSQADFISLNCDLNPTSRHLI
                     HKDIIRLMKPDAVLINTARGPVVNEKDLIEALQQKRIAGAGLDVFEEEPLPADSALLH
                     MENVLLAPHNSNSSPRAWENVHWNSIRNLLIGLAINAEDLDEWKSKV"
     assembly_gap    26481..26580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26581..27078
                     /locus_tag="PLW25_00535"
     CDS             <26581..27078
                     /locus_tag="PLW25_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS53003.1"
                     /translation="LIETTVEEWDKVIATNLRPVFLSAREFHPLLINAGSSAIVNMSS
                     VHAIQTSASISAYAASKGGMLALTRAMAIEFAKDNIRVNAVLPGAVDTPMLRSGLDRG
                     HVTGPSLNERLDNLARKTVNGRIGQPAEIARAVYFLADGTQSSFMTGQALVVDGGATA
                     RLSTE"
     gene            27085..27888
                     /locus_tag="PLW25_00540"
     CDS             27085..27888
                     /locus_tag="PLW25_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOS53004.1"
                     /translation="MRAKIKQHIPDPLVDLYRSARDNLSLAFAYPNARFHPWRVDSIQ
                     KLEALRDSHGGERCVIIGNGPSLKQTNLQKLRGTFSIGMNRFYMAFNDLGFTTSILLT
                     VNNLVIEQCADELRGLPIPIFVSWRGRRYIHPAPNLHYLYTSYIKPSFNGDATGRLWE
                     GATVTFVAMQLAYFMGFKQVILIGVDHNFTSTGKPNTTVVSTGADPNHFHPGYFGKGF
                     RWQLPDLETSEIAYRMAKSAYEKNGREILDATIGGKLTVFPKVNFDTIW"
     assembly_gap    27903..28002
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28003..28628
                     /locus_tag="PLW25_00545"
     CDS             <28003..28628
                     /locus_tag="PLW25_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53005.1"
                     /translation="SFVYGDVQVVNEKDELINILRYGNWGLKELMSFHIIGQPAVFIR
                     RAALQKAGYLDLSYQFLLDHQLWLRLALHGGMTHVPQLWAGAHYHAGSKNMAQAALFG
                     NEAQRIVQWMEATPEFASLYAQNSRRICAGAERLNAFYLFDAQDYRASLDAYLRSLAL
                     HPAAAAQDWYRILFAALSPLGLDQLKKAHLARRKKRLKSFRPPLTRK"
     gene            28628..30172
                     /locus_tag="PLW25_00550"
     CDS             28628..30172
                     /locus_tag="PLW25_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOS53006.1"
                     /translation="MSDSAPTSTALFFKPSKAAVLILLLIFLLAGLGVRLIDLDDLPL
                     DFAATRQLHSFIMARGLFYELDTPITRAIPEDIRQFAITTGRSEPLIEPPVMEYLSAY
                     IYAVIGKESMLVPRLLSIAFWIVGGIPLFLLMRKITTLNGAFISLALYLFNPFAIVAS
                     RSFQPDPLMIMLILWALYFQFKWMEDDKLASAIFAGIFTGLAVLIKTPAVFFVGIPMI
                     GVVLLKGLKKSLRDWRVYLVAAISLLPAVIYTALSATVGGNAGAIFGARWVPALFSDP
                     KWYLNWLMLARLVVGFFPIVISLLGFFLIQDKKYQALYFCMWLGYLLYGFMFAYHIYT
                     HNYYHLPLIPVVAMGFGVIGGELFSRLESKNLSRFFRLLVLLLFLFAIGLNVMKARGE
                     MLGKSYRHEAKYWKELGEKIGQNANTIALTHDYGYRINYWGFVQPTLWPTQGDMTVKK
                     LIGSTDPEFEALFKMRTEGKDYFLVTLLGDFESQADLHQYLFENYPYEQGDGYYLFDL
                     KHPLGN"
     assembly_gap    30320..30419
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<30420..31435)
                     /locus_tag="PLW25_00555"
     CDS             complement(<30420..31435)
                     /locus_tag="PLW25_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS53007.1"
                     /translation="MSKRLDWVQEEIDTLKAAGLYNHIRTLGSPQGAWLVVDGKEVLN
                     FCSNNYLGLANHPIMVKAAQEALKKFGVGPGAVRSIAGTMQLHTELEQRLARFKGVEA
                     VVVLQSGFAANTAVIPVLVGKDDLIISDELNHASIIDGCRLSGAPIAKYAHTDVADLE
                     RVINENEGKYRRALVITDGVFSMDGDLAPLDKIYEVTSRHDIMLMVDDAHGEGVIGRG
                     GRGIVDHFGLHGKVDVEVGTLSKAFGVVGGIVAGNARIIEWVRQRGRPFLFSSATPPA
                     DVAACIAAVDLLENSTEMVDRLWDNARYFKKQLKSIGLDTGQSVTPITPVMLGEAPLA
                     QQFSR"
     gene            complement(31479..33113)
                     /locus_tag="PLW25_00560"
     CDS             complement(31479..33113)
                     /locus_tag="PLW25_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53008.1"
                     /translation="MPGLRSTLANYDLDFLTRIARRWDVQISQREVESARQDLLENML
                     DTSLFEKVLEGLDDGAAKAWNHLVQKGGKIHWAEFSRIYGQIRDYGRASREREEPDLH
                     PVSAAETLWYAGLAGRAFLRTEAEPKEFFYIPDELLEVAKNTGKPAPKLHIRPSVNQK
                     PKWVARAEALLPDRVTDILAALRMRREIPAEIWNAWDIPRDFLYPWLREVGLVDDTLQ
                     PVPEALKEFFNATRGQVLTHLYQVWLKSNEINELRLLPGLEFEGTWKNDPIPPRRLLT
                     EILAGLESGTWWSLSSLLATIKETNPDFQRQGGDYESWYIRETKSSTYLSGFENWEKV
                     EGALLTYLLGAPLHWLGIVNLARAGENGKITAFQLNEKMKPLLDGAELSSSDEENRVM
                     KIRDRLLFSIPYFAPRLPRYQVARFCELVHVFPKESLYALKTDTLKKAEEQGLRLGQL
                     IQLLEREKAAAIPENLRVLEERWSQHGQEAVIERVLLLRFTREDARAAFLKQATGRFA
                     LETLDPLCVVINEKQVDGILRLLNELGILAEIAGDV"
     assembly_gap    33385..33484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33485..34221
                     /locus_tag="PLW25_00565"
     CDS             <33485..34221
                     /locus_tag="PLW25_00565"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOS53009.1"
                     /translation="LYVERPGESARVLFHNGKLEYFESSNEKPELLRSLKDQKLITAY
                     QHTLLKERLEGFSDREAGIYLVNAGYATLDQVTTALEEDYAKKFRALFDWNEGLFRFE
                     AGELLPENKIPVHLNLENLIVEGARELQEIEELKAEIPSLDMAIKFTDRPGTDIRNIC
                     FSPTEWQVISYVNPRNTIQQIATATKLDEIEIRRVVFTLLQAGLVEIIRPGGVPLAIP
                     GKNLPVRNPEENRSLVKRLIDRIRSI"
     gene            34243..34818
                     /locus_tag="PLW25_00570"
     CDS             34243..34818
                     /locus_tag="PLW25_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HOS53010.1"
                     /translation="MQTQTVKIVVTGPFNAGKTEFIRSVSEIDVVSTEKRISATAEKI
                     KESTTVAMDFGRITVDNDLVLFLFGTPGQKRFDFMWEILSEGMLGFIVMVDSTRPETF
                     REARSILETFKAYAPTPYLVTANKQDLYDAWDTEDMRLALRLDPSVKILPCEATKKSS
                     VKRVLLELLYGILKEMDDNEARQNNQEDEIS"
     gene            34820..35320
                     /locus_tag="PLW25_00575"
     CDS             34820..35320
                     /locus_tag="PLW25_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53011.1"
                     /translation="MSQTMLEYKNVGLVEVLGAEAIADFAKAMEAQPCDPIVHGILVR
                     AGRAGFHYWLTQEGDTLEKADASFRLAPIKRKINLGLQHFCAILSTDTKYALEFKDSA
                     NKWELALTAVEPAGLFSLESSYLSGFLQEFASWAGMGRLYQVHKKVGDSPHAQHSIII
                     EKEPVE"
     gene            35377..35934
                     /locus_tag="PLW25_00580"
     CDS             35377..35934
                     /locus_tag="PLW25_00580"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOS53012.1"
                     /translation="MTEDVFICVDVETAGPVPGKYAMLSIGACLVFDTTATFYIELKP
                     DKPEVDPEALAISGLDMDALQQNGVEPAEAMRAFADWVKVVSPAPAKPLFVAFNAPFD
                     WMFVNDYFHRYLGRNPFGHSALDIKAYFMGQMGVNWADTTMRRISSFFLEEHRLSHNA
                     LRDAQDQAEIFRRILEANPRFNLAE"
     gene            36009..37340
                     /locus_tag="PLW25_00585"
     CDS             36009..37340
                     /locus_tag="PLW25_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53013.1"
                     /translation="MAKTNDSHKADLNRIIESAKRMGVEIKEDEALQWLTNMAAYKES
                     GQISLDTRSGVFGHNITMLDFSDKELEYFRRIGKIVEFEDIPGKVETALALSGSSAQS
                     KIQTYPGDCDYFERINIIAPTRDEACNILAEIMRKKALDYLQGPNYQLIEVKFGEYPQ
                     DIVLGTREVKAGSPISWDPAQIREGRITGFKKDGTPVTVAWDDVKNNPGWCKLDWVVA
                     DPIRKQLANASNMLDVTWEAPGGDITPLDGYLDPYFQEVYLEASSVPIFSKLSQHVSA
                     NALEEYVNALEREVYKYLTKDVNYGKVAKRMYNIFRLTGKYEEAAFLRELFDEPSTVL
                     YQIWSLIRTIDDAYIPGSSITNEQMVESTDRLILEVIRALEGDEETEIVKHLLNLRTA
                     LANPDARDMLTAEAEAARARVINIVNNFFYEKLVAYPTIKEYMDEVQTRSH"
     gene            37530..38096
                     /locus_tag="PLW25_00590"
     CDS             37530..38096
                     /locus_tag="PLW25_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOS53014.1"
                     /translation="MNNSHQSISEGVAVELYLDAAAEEQVLKFRELIYREGVQPVQGL
                     MDDKPHISLAVFPITDPAAVIDLTREFALTVPRFSFRLGAVGTFPTQDNVLFLSPVPS
                     VPLLQVHAAFLTRVKDAGINCSPYYFPGVWVPHLTLEFEISNDELCRSMKVFKESFTP
                     IDGEFTQLGVVQFRPIQYLDQFDLQKGN"
     gene            38096..38545
                     /locus_tag="PLW25_00595"
     CDS             38096..38545
                     /locus_tag="PLW25_00595"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HOS53015.1"
                     /translation="MSEGVPPPSEEQIRDAVNRRKTKAEEYLQDPDKTRRLLEEAVHK
                     ANDHEGKPGQKLDFWLQLKAFFRLLRAYTNREYTVVPWGSIVMVTIAILYFVSPIDLI
                     PDWLPLAGFVDDAAVILFVIRQLKQDIDGFLAWENAQKNPGGQIIDL"
     gene            38617..38994
                     /locus_tag="PLW25_00600"
     CDS             38617..38994
                     /locus_tag="PLW25_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53016.1"
                     /translation="MSKKWVTVLLVVFVLMFALSACGSLKDVSKLGDKFMSALKTQDN
                     ETSYAMLSPDIQYEVGYEAGWAEWTSIRAFNDWKFTSKSVENNLATLEGTAHLDGEEY
                     LVTLAFTRNGDAWEITTIIFELP"
     gene            39206..40141
                     /locus_tag="PLW25_00605"
     CDS             39206..40141
                     /locus_tag="PLW25_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOS53017.1"
                     /translation="MKINLLAFSADRQLLTSLEVITFNGITLTTTPAGTSSELRALTQ
                     QQRFDAALIDAADGHLPVVQTILNLTAQQPGIKILLLVADEQNETLPPEDIAYDRLLS
                     RPLSPEQLISSLKELFLQPEETCAETGAAIRETPAAQAEQEPSLIEIIDAAENENRLT
                     GEQGGALTSHTVKSAGNADPNLIVKELRLSYCCVLLPWSPHQYLTGELSDHAADFLPR
                     LHHSLGWRMTGISIRPQYLQWVISLPVETCPVTAIQKIRQRTSANFFKTFPDLPSSGE
                     NRDFWAPGYLMLSGVQTVPSAIIHEFVKRARPADG"
     gene            40162..42948
                     /gene="polA"
                     /locus_tag="PLW25_00610"
     CDS             40162..42948
                     /gene="polA"
                     /locus_tag="PLW25_00610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOS53018.1"
                     /translation="MPPILYLIDGHALAYRAYYAITAGMSDRMQTKTGEPTAGIFGFA
                     NVLMRLLEQEKPEYLAVAFDTGKTFRNELFPDYKATRAKMPDDLRPQIERIRQMIDAF
                     HLPRLEKEGVEADDVLGSIATQAVEQGLGVKIITGDRDLLQLVNDRIIVNLPGSRLAD
                     AKNYSAADVKEYLGVEPEQVVDYKALVGDTSDNIPGVPGIGPKTAVTLLQTYHTLDAL
                     YQHLDEIPGKTGEKLRVGKESAYLSQELARIKKDVGVRLVLEDARTDHLDIASVENLF
                     RELEFRTLIPRLRLIAAPLKKQETPQLSLFGEEMKEIIVPESSYMIEVKLIDTAQKLA
                     SLKQALDASSLIAFDTETTALDPLQAELVGLSLAVKEGEGYYIPLGHKGEQPQLKVSE
                     VIEALTPALTNPSIEKAGHNIKYDALVLARYGLRVSPLSFDTMIAEWLTNPASRDLGL
                     KDLAGTLLGIRMTRIEELIGRGKNQLTMDEVPIAAAAPYAAADAEVTLRLIPLLKEKM
                     QKTNSTGIFNTIEMPLVPVLLKMEQEGISLDIDFFKRFSAEIGERLRLIEDEVHQAVG
                     HAFNLNSSQQLSKVLFEDLKLEPPDRNKRTSSGHFSTSAAVLDELKGQHVVVDLLLEY
                     RELSKLKSTYLDALPQQINPRDNRVHTSFNQTGSVTGRLASSDPNLQNIPTRTETGRQ
                     VRLGFIAAPGHRLLSVDYSQIELRIVAHMSGDEAMLKAFRDGQDIHATTAAAIYDLPL
                     EQVTKEQRRHAKAINFGLIYGMSAFGLSRSTDLTLGEAENFVRDYFEHFPGVKKYLDQ
                     IRVLAARQGYVETMLGRRRYFPNLANPLNQMMRNREEREAINAPIQGTAADIMKIAMI
                     RVQRALEESASRARMILQVHDELVLEVPENEIALTSQLVRGIMEAAYKLDIPLLTEAR
                     SGVNWGSMQVIASE"
     gene            42967..45288
                     /locus_tag="PLW25_00615"
     CDS             42967..45288
                     /locus_tag="PLW25_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS53019.1"
                     /translation="MNVTDQALQAKLNNGHAALWEHDWDNAVRVYTEVLLSAPENPIA
                     KASLGLAKFHQKKYIEALQLFQQLAAQYPEDPMPMERIARIYEREGLLAEASTSFFRA
                     AELHLKAKDVEKSIADFQAVIRIEPNNQIARARLALIYERLGRKNEAAAEFLDLAAVI
                     QQHGNIDKARLMLEYALKLKPDAVEVHNAMASLKKNEPLRLRDRSGEPTGAARMAQVR
                     EIETAVQEPEELTGNDPMTEARVMALKEMAGFLFEDQETNRPAVKPRDLQNFVPASSA
                     SEKAEAASHKRVLYHISECIDLQTKGENLEAAAELERAININLDLSAAHYLLGYLLRE
                     SDPGKSYPHLQKSVMDPAYALASNLIMGEIHFEAAQLKEASSNFLHALMLADAETVPP
                     EEAGDLLQLYDPIFESLAFITHERDLRNLCAVIREQLSRPDWRDYLKAAREQLPPAPE
                     GSPPLPLAEMLMDSTSSQVVESLAEVRQLAMQGKNRSAMEEAFRALSYAPSYLPLHIQ
                     IGEILVNEGRIQEAVEKFIQVARLYTVRGDAAQAIRLLTRVTRLVPMDISVRRNLIDL
                     LRSNGRNAELIEQYMDLANVQYLLADLEQSRNAYHSALMLARQSRSTREQSIKILNRL
                     ADIEMQSLNYKEAVKAFEQLRSLSPLDRSTRVALVDLYQRLEMESAALNEVDAYIKLL
                     EDENRLGDAENFLDAILSEKPDSDGLQRCMVSYYTSHKKTSTAIEKLDKLAEKLLVEN
                     NVKGSLAVVEKIISLNPPNQTEYERLRAELLKR"
     gene            complement(45350..46303)
                     /locus_tag="PLW25_00620"
     CDS             complement(45350..46303)
                     /locus_tag="PLW25_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53020.1"
                     /translation="MRKLIRLLVIVIVSALLGASSSGAVRAQESDSVYVPETGHWIWG
                     EFLRTYNSVSDPLLYFGYPITDDFTDPISKSRVQYFEKARFDLVETPSGPVVRVAPLG
                     ELLHEAGNPLANIPREGPACKAYQTGFTVCYAFLQYYEAHAGETHFGMPLSNVEVEDG
                     RYVQYFTRARMEWWPDRAAGERVVLTDLGKLYFDRVVADPELLKSNPPPNLSGSLMTP
                     RVRVFTQKSLIGVEESQSVFVVVQDQYARPIEGAQVGVTLSFMPDTREFYRLPETNEH
                     GISQFTFTTPQIPVKSVVKVLADITFRGQHGSGRSWFRIWW"
     gene            complement(46330..47637)
                     /locus_tag="PLW25_00625"
     CDS             complement(46330..47637)
                     /locus_tag="PLW25_00625"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS53021.1"
                     /translation="MRVRIMRVISRFSTILKKYPRQFWLVSLIMLLAWLFHSLIWPYL
                     MLYISQRLGEPLSSVAWLMTLNAAVGMVTTFMGGAIADRFGRKGVMVFSLIFSALGWF
                     LFRAAGTLPVFALLLVVTGASTPLYRLASDAMVADLIPEANRLDAYSVLRMGNNFGVV
                     LGPAIGGYLASISYGITFGVIGVGFGLIAVFVTLLIIETKPASGKPVVVTAPTSGGYL
                     RIFKDRVFMVMMGAYTLNRISTSILWLLLAVYAKQNFGVSEKMFGFIPMTNALMVVLF
                     QLVVTRQVNRFRAETAMTTGALIYAIAIFCVAFGSGFWWFWLCMVAATIGEMILVPTT
                     TTFTASLAPEDMRARYMSLYALTWGIGTGLGLLLAGYVSDVFYSRAMWYAAGLVGFAG
                     FLAFSFIARLKKTNPHGFMHPSAEDVQARGQRLARGKTRSDCE"
     gene            47728..48165
                     /gene="mscL"
                     /locus_tag="PLW25_00630"
     CDS             47728..48165
                     /gene="mscL"
                     /locus_tag="PLW25_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOS53022.1"
                     /translation="MLEDFKKFIMRGNVMDLAIGVIIGGAFSKIISSLVDDILMPPIG
                     LLLGKVDFSNLFASLTSARYATLAEAQAAGAPTLNYGVFINNVVDFLLISLAIFLLIR
                     AINRLTKKQAEAAPVTRACPRCLSQIPLKASRCPACTSEVTPQ"
     gene            complement(48298..49638)
                     /locus_tag="PLW25_00635"
     CDS             complement(48298..49638)
                     /locus_tag="PLW25_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS53023.1"
                     /translation="MPTSSSPDEPSAPSEVCPICGGVGFLHRDLPLEHPEFGKLVPCV
                     CQQNKMIEAARNRLYELSNLAAFKNMSFSSYSVQGRLGLADEQIRSLQYALSQAQQFA
                     AAPRGWLLLMGSYGCGKTHLAAAVANTCVEFGMTTIFLTVPDLLDWLRYSYDATDATF
                     EQRFEEIRNVPLLVLDDLGAHNATQWAAEKLFQIIDYRYTRKLPTVITTNLDLEDLDD
                     RIRSRLQDPDLVTTVHINAPDYRSPVKERRDPLLYQLQLLSDRSFGNFSLREPEQLAP
                     EVQLNLEKVFRAAQEFAENPRGWLVLTGTYGCGKTHLAAAIGNYRKGMGDAPVFVVVP
                     DLLDHLRATFNPTSGTSYDDLFSRVRSAPILILDDLGTQSATPWAREKLYQIFNERYN
                     AKYPTVITMSTTLEDLDARIRSRMLDTRLCSIIEILAPSYRVGVIKTPARKARS"
     gene            complement(49616..50308)
                     /locus_tag="PLW25_00640"
     CDS             complement(49616..50308)
                     /locus_tag="PLW25_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOS53024.1"
                     /translation="MPEFDGFTAGRNESIPLPATFFTHLLTVIDNLDEMRITLYAFWY
                     LSGQIKEPRYLGLMDILQDNLLMASFGTTTDEQTTRLEEALRRACDRGTLLEARFKDK
                     RFFFLNGEQGKAALVGLKNGTWDPETAQSLPLRLQRERPNIFSLYEQNIGPLTPILSE
                     TLQDAEATYSAEWIEDAIRIAVTRNVRNWQYIEAILRSWKEKGRDGTDRKSAEEKRKR
                     DSEGKYADFIIT"
     gene            complement(50319..51674)
                     /gene="dnaB"
                     /locus_tag="PLW25_00645"
     CDS             complement(50319..51674)
                     /gene="dnaB"
                     /locus_tag="PLW25_00645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOS53025.1"
                     /translation="MSDFDDAATVIQSQPYSREAEEAVLGSVLINPESYYEISEVIKP
                     DDFYIIRNQWIWRSYIRLHERRVPIDILTVSEDLQEQGQLGEIGGQAYLLGLVNQTPS
                     SLHGAAYAQFVEQAAIRRRMLTAANEMARLAYDQEKDIRTILDEVEKSVFSLGASRVR
                     HDLKPVNEVVSEYYDYVDEQSKRDEELSGVPTGLYDLDKLLGGMQKSDLLIVAGRPGT
                     GKTGFLLSVAKTAAQKYKKNVAMFSLEMSNQQLVQRLIAQETGIDTLRLRTGKLQDHE
                     WDVFTKAIDVLSATNIWLDDTPAITPLAMRTKCRRLDNEHKLDLVLVDYLQLMSSDSR
                     NENRVQEVSYISRSLKALARELQVPVLAAAQLSRAVESREEKKPVLSDLRESGSLEQD
                     SDIVIFIHKSKESDKTTNLIVAKHRNGPTSPGIGVVFLEKLAKFVNAAPAESEPAPEK
                     R"
     gene            complement(51696..52316)
                     /locus_tag="PLW25_00650"
     CDS             complement(51696..52316)
                     /locus_tag="PLW25_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOS53026.1"
                     /translation="MVEVVIDSVRVSLTNQQRIVMLRESGRERYLPIWIGPYEAEAIT
                     ISLQEIEVARPQTHDLLLQSLTKLGARLIRVEVVSLKKDVFYGNLVLELDGKLYDVDA
                     RPSDSIALAVRAHVPILVNSEILREAGLTPEVDIQAEVLDVEDEPTPEAESDMESEET
                     SKRLSLFEEFLQNVNLDDVAGDSGTQEDDSAAGEGNQGNQDDKPRS"
     gene            52626..53315
                     /locus_tag="PLW25_00655"
     CDS             52626..53315
                     /locus_tag="PLW25_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS53027.1"
                     /translation="MSKPRILLIEGKRTDHPSFAAGLTKKGYLVESVPSGAAALAQLD
                     LAVPNLIVVDASSMRTNGKRIVQALRQKQPGLALILVIDSNSDPNDHYDADVILVLPF
                     TMQKLINRIRHLLPGQNNYDLVAGPISLDVTEKRVTIGDRQARLTPRLTILLKILMQH
                     AGEVVERKALFSQAWETDYTVDTRSLDVHMSWLRQAIEENPRRPKLILTLRGVGYRLA
                     VEPITKPRQRK"
     gene            complement(53434..54738)
                     /locus_tag="PLW25_00660"
     CDS             complement(53434..54738)
                     /locus_tag="PLW25_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HOS53028.1"
                     /translation="MNRKTEKGQVLVIIALAMIAILGITALAIDGSLVYNSRRADQST
                     ADSAVLAGAGAASQVLKAGNLSQFTCGSTLTSQAATAAVNAARLSAQDDGVDLLPFDL
                     STNNGVDVECGTEAGKTYLKIKAIVSTISEQRFGQVITNQPLRTTVEATSRVSLSGST
                     GGGMGIVSLSPNCGVQGGFDPASKPINVIGGSVFSNSCLYGNGPVTVYGGGTISYIDT
                     DYSSNSTPAPQRATQPVSINIPPPDCSGLLNRGSSTKGGTISPGNYTFIQAPGKTPLI
                     FSPGLYCINSTSLNAFDANAGARVTAQGVTLYIKDGNFNVGSNVQLLMSAPNCSGGST
                     PTGVPPGVCGLLIYMDEPPSNPNQKSTQEMNLVGISGSTFSGTIYVPYGTIDVAGSGT
                     TTTYETQLFGYRIITTGSGKVIFDVSGSNFYSQPGLSIDLMK"
     gene            complement(54770..55210)
                     /locus_tag="PLW25_00665"
     CDS             complement(54770..55210)
                     /locus_tag="PLW25_00665"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOS53029.1"
                     /translation="MRIFRYKNRVINSEQGQSLVELAIGILILLILLSGIFDIGRMIF
                     VQFALQDAAEEGIIYASGFPTECNNINNRVRNSSSDLPKDGSVAVSILINNRACGVGT
                     PPKAGDKIEITVTQPFPITMPFLGALTGQTVSLRATANGIVLRP"
     gene            complement(55290..55799)
                     /locus_tag="PLW25_00670"
     CDS             complement(55290..55799)
                     /locus_tag="PLW25_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOS53030.1"
                     /translation="MNFMLLWLRSKDRRIAKPAQGLVEFALIIPLFLMLLYGLFEVGR
                     AVFMLSAVRNASRDAVRYAGASGTNPSGVERYRDCAGIRDRAKRVSAFVDLSAVNAVE
                     ISYDKGPSTPPISCEALVSSGKKLALGDRVVVTVRATFVPFVGPLLNFNTIQIESTSA
                     RSLVIGLDV"
     gene            complement(55915..56751)
                     /locus_tag="PLW25_00675"
     CDS             complement(55915..56751)
                     /locus_tag="PLW25_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HOS53031.1"
                     /translation="MGVPLSPIQLIFGVLLAALVVYAAYRARSLDRSGALAAFLLGVV
                     VFGIGGFCWALVLLTFFITSSGLSHLFRVKKQKVEEQAVKGGRRDAWQVGANGGVAGL
                     AALLSLVMPGSVLPWLAFAAAFAAANADTWATELGVLNRSWPRRLTDWKRVPPGTSGA
                     VSLAGTLAATGGALLVALMAAACWPVVNTSFREKLVWGLVILLAGVFGSLVDSLLGAT
                     LQVIYWCPACEKETEKHPLHSCGKPTRRVRGWGWLNNDVVNLACTLSAALLACLLGLL
                     LR"
     gene            complement(56753..57664)
                     /locus_tag="PLW25_00680"
     CDS             complement(56753..57664)
                     /locus_tag="PLW25_00680"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOS53032.1"
                     /translation="MSQRQAFYPPKKAGILLHAFLSLLLLGGIAFLLVLAFSQPPSWR
                     LVFYLLGVILLGMLLPLAAYRGYALLKADYSLERDGLQVRWGLRSEDLPVTDVLWVRP
                     ASDLVKPLTLPRFALPGAILGTSHHEELGEVEFIASSARHLVIVAALEKTLILSPADP
                     EDFSRRFQRVIEMGSLSPLPAHTTIPAAYMNQVFKDPLAKVLMVLSAVLTLLLLLATG
                     LLIPSREVVSIGYDFNTLPMPPVPSNRLLMLPVIALFFVLLDFIAGLFLYRRAETRAI
                     SYLIWAAGILSPLLLLIAVLLTTMVRV"
     gene            complement(57678..58865)
                     /locus_tag="PLW25_00685"
     CDS             complement(57678..58865)
                     /locus_tag="PLW25_00685"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS53033.1"
                     /translation="MKNVEPVVPMSLPDLSDAEREAVMEVLRTPHLSMGPRIDDFEAA
                     LAEFVGAKEAIGVSSGTAGLHLCVRAAGIQPGDLVLTTPFSFVASTNAILFEGGIPVY
                     VDVDPKTGNMDPLLAAQALEDLRKGGAAARRWLPRKGAGDTARLKALLPVDVFGQPAD
                     YDLLQPLAEEFHLKHIEDSCESLGAEYKSRRLGLMGDYGVFAFYPNKQMTTGEGGIIV
                     TDDADAARYMRALRNQGRAPGDTWLQHTYLGYNYRMDEMSAALGLAQLRRIEELLNKR
                     QQVADWYASRLANIDGVQIPLIAPETTRVSWFVYVIRVAPGLDRDALAEKLQLRGIPV
                     RPYFLPIHLQPYMVDKFAYQLGDYPVTEDLGRRGLALPFSGKMTEEQVDLVCRVLAQC
                     VTK"
     gene            complement(58906..60675)
                     /locus_tag="PLW25_00690"
     CDS             complement(58906..60675)
                     /locus_tag="PLW25_00690"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOS53034.1"
                     /translation="MSLFNYLEQRMRLSNLFSQTLRENPADAEFASHQLLVRAGFIRQ
                     LGSGIFTYMPLAYRVLERVKRILREEMEAIGGQELLMPVVQPADIWKESRRYYQIGSE
                     MGRFTDRSGHEMVLAMTHEEAVSDLVRGVVHSYRQLPAIIYHLQTKWRDDPRPRAGLI
                     RVREFLMLDSYSLDADWQGLDTRYKQHYEAYFRIFRRCGLPVVAVRSDSGMMGGEEAH
                     EYMYLTPGGEDSLLFCDACGYSANRQAATTRKMEPAKEAFLPLEKVATPRVSSIDDLT
                     AFLNIPPARTAKAVFYMGLVEEDGGEQKWAFIFTVVRGDMEVNETKVANLVKASDLRP
                     ATEDEIRSIGAVPGYASPLGLKGVTVFVDELAAKSANLVAGANEEGYHLRNVNHGRDY
                     QASMIADISAAREGDACPECGAALRLSRGVEVGNIFKLGARYSDALGCTYLDKNGQSQ
                     PVIMGSYGIGLGRLIACVAEEHHDERGLAWPVSLAPFPVHVVVLKGKEMDSEALAARV
                     ENALRDAGLEPLVDDREESAGVKFNDADLIGLPLRITVSERSFRVGGLEIKPRRGGET
                     VVVPMENLVVEVQRLLNALEEPL"
     gene            complement(60672..61421)
                     /locus_tag="PLW25_00695"
     CDS             complement(60672..61421)
                     /locus_tag="PLW25_00695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOS53035.1"
                     /translation="MDVKFWLGYLSEWLGVIAVVMIAGTSPLLRKIRRIEFRFSRREA
                     AFALTLFALIFFFAFQVYDNNLLGFLKTAAGAFVGGDVALRMLIAVIALLPAIILLLA
                     RGQPLKSTGWSKENTRASLMLGLLLAVLTIFLRGKFTPLLKGFTEEKGSLLLVLLILS
                     LAEESIFRGYIQLRLMSYLGTTWGWLATAFLYLLWQLPGRAWLSHFPTAWPEVLITLA
                     QALILGWIMRKSYHVAATALYRAVAAWLMVI"
     gene            complement(61427..61912)
                     /locus_tag="PLW25_00700"
     CDS             complement(61427..61912)
                     /locus_tag="PLW25_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOS53036.1"
                     /translation="MFKKPTPATQEQTAPIERVNSVLGPGINWAGNLGGRGGIRIEGT
                     FEGEIRIQGLVVVGETGRVTCQNLRANSVIVAGALRGNVTAEKLEIRSTGRVWGDVVT
                     IAFATEEGAFLRGQVRMEEKVDLHLEEPSEAVKDLDEPADEKRMDTPKRPARGRQVAG
                     D"
     gene            complement(62034..63122)
                     /locus_tag="PLW25_00705"
     CDS             complement(62034..63122)
                     /locus_tag="PLW25_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HOS53037.1"
                     /translation="MSEPLQILVPLINPNEPEALLAELRVREGDKVTAGQVLASFETT
                     KSTYELTAERDGFILNLRFKAGDTLRSGERLCYLADSIGQPLPHETAKPAADRLPRQE
                     ELPQGLRITQPALALAKTNQVDLEDLPRGVLVTEEMVRKMRPIPVEDVDPSALIIYGG
                     GGHAKSLIDLIRLLGQYRIHGILDDGIAAGTRILGIEVLGGAELLLGLRRQGLGKAVN
                     AVGGIGSIVPRLAVYEKLAAAGFECLTVVHPRAFVEPNARIAEGCQVFFNAYVGSEVE
                     VGFGSIINTAAVISHDCRLGKTVNVSPGAMLAGAVTLGDRALIGMGATINLGVTIGAG
                     ARVGNSAVVKADVPENGIVRAGATWPAD"
     gene            complement(63115..64164)
                     /locus_tag="PLW25_00710"
     CDS             complement(63115..64164)
                     /locus_tag="PLW25_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOS53038.1"
                     /translation="MNTTVLSSLNAGLLRALQKDEHVYLLGEDILDPYGGAFKVTKGC
                     SSAFPERVLAAPISEAGLAGVSAGMALRGLRPVLEIMFGDFITLTADQLINHISKFNW
                     MYDHSVCLPMVIRAPMGGGRGYGPTHSQTLEKLFIGIPGLTLIAPLNLTGKGGTQQPG
                     ELLEEVILHTEEPVLFIENKLQYLLPVYQPQDLPELHWLVEQPVKNTLMKTPTYLVKV
                     SGAPAARASLAAYGYMAELARQAMLKLAYEDEIFVELVIPTRLAPFDFTALADSIKRT
                     GRLVTVEEGNLTGGWGAEVMARALEEVGGRLRAAVRVAARDLPVAAAPSLENEILPDV
                     EDIVQAVRKVVGRNE"
     gene            complement(64161..65057)
                     /locus_tag="PLW25_00715"
     CDS             complement(64161..65057)
                     /locus_tag="PLW25_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOS53039.1"
                     /translation="MDEITLYRTMYRIRRFEETVLEEFKRGVFAGTTHTSIGQEANAA
                     GVIAALDPQDIIVTNHRCHGHFIAYGGDPRALFAEMMGRQTGVCGGRGGSQHIHWRNL
                     YSNGVQGGIMPIAAGMALAEKEKKSGAIVTVFIGDGTFGEGVLYEACNMASLWKVPLL
                     IVVENNHIAQTTPTTLAMAGNIAGRLQAFDIPVTELDSCDVMEIHLNAMQMTRLVRRE
                     GRPYALVLNTARFGPHSKGDDTRAEAEIQALRSSRDPLLISGSRLAKDELERFQGETD
                     EEVNGAFTRALADPPARLEEGG"
     gene            complement(65057..65662)
                     /locus_tag="PLW25_00720"
     CDS             complement(65057..65662)
                     /locus_tag="PLW25_00720"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOS53040.1"
                     /translation="MGIILDMNCLPQSFYLGEVTGVARALLGKRLVRQVDGRRVSGII
                     CETEAYRGEDDLACHAHSGKTRRNEVMYGLPGRAYVYFTYGMHWCLNTVCGEEGYPAA
                     VLIRAILPEEGLEWIAARRAGVEPANWCNGPAKLTKALGVDGGLNGTDLTDANSPLFI
                     EEGYIIEQAQIRTSPRVGIQSAPEPWRSMPWRFTCSCRAGT"
     gene            complement(65738..66676)
                     /locus_tag="PLW25_00725"
     CDS             complement(65738..66676)
                     /locus_tag="PLW25_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOS53041.1"
                     /translation="MRYLITGAAGFLGSALANRLTAAGDVVIGVDDLSAGDQKNLSPT
                     VQFVRGDVNDRPTLWSLLQEVDCVYHLAAKVSVQESVLYPREYNNVNVGGTVTLMEAM
                     RDVGVRRVVFISSGTVYGNQPVQPVKETVIVNPRVPYAVSKLAAEYYVKTIGSLWGIE
                     TVCLRVFNAYGPGQRIPPVHTPVIPGFLHQAWENGTIVIHGDGNQTRDYVYVDDVVNA
                     MLAAANAPDVNKLTINVGSGTETSVRDLARMAIEVTGGNPEVVYNPRNEGGISRLRAD
                     ISLARELLAYEPEVELLEGLKRTLAHDTATDKTQYE"
     gene            complement(66724..67728)
                     /gene="add"
                     /locus_tag="PLW25_00730"
     CDS             complement(66724..67728)
                     /gene="add"
                     /locus_tag="PLW25_00730"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOS53042.1"
                     /translation="MISQDIPLVELHRHLDGSIRLETILELGLKHNLPLPGKTLDTLR
                     PFVQVTTPQPGVMAFIEKFKWMTGVMVDYDSCRRIAYENVEDACREGIDYIELRYSPW
                     FMAEAHGLQPEGVVEAVTEGIRAAEQEHPIRVNQLGILSRQYGPQIAMKELEAELRYA
                     HEFAGLDLAGDEEGFPGELFRDHFRKARDAGWHITVHAGESAGPESIWQAVRELGAER
                     IGHAVRAPEDPALLDFLREKGIGVECNLTSNVQTSVVSDYAAHPMRLFMEKKILASIN
                     SDDPGISAINLRYEYEVAAPKAGLSPAMIRQAQENALLTAFLTEEEKTALRRKKSTGA
                     "
     gene            complement(67725..68231)
                     /locus_tag="PLW25_00735"
     CDS             complement(67725..68231)
                     /locus_tag="PLW25_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53043.1"
                     /translation="MKASKVAIILSLCLWVLTACNGGEQAGLNQETGVFPKTDWSTIP
                     EQLPASLKGYELYSWQQGKTRVYTLTTGTNRTKSFAEITAAENIWEGDYLKISVASLE
                     ELKKLLSRLPAETQVFWGGIDLSGQVEEGTLYFSYPADEELKEILQYSQELGLKLHTI
                     HEQQEDEP"
     gene            complement(68243..68686)
                     /locus_tag="PLW25_00740"
     CDS             complement(68243..68686)
                     /locus_tag="PLW25_00740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS53044.1"
                     /translation="MTIQINEMSVLHIQAALALWKQIEGMGLSSADEPGALARFIQQN
                     AGLSYIAEEDGQLVGTCLCGCDGRRGYLYHLAVLPQYRRRGLGSALVRTVFTALHKRD
                     IHKCHIMVYTTNHSGLKFWQADGWVRRPEIVLMSKEIEVRDGPAA"
     gene            complement(68667..69269)
                     /locus_tag="PLW25_00745"
     CDS             complement(68667..69269)
                     /locus_tag="PLW25_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOS53045.1"
                     /translation="MEDTLTVIMKRRSIRKFTDQPVEKEKIIQLLEAAMAAPSAMNAQ
                     PWEFVVITEKSVMDKFRRALMFAKMNAPAAICVLGSSRMQKGKVGDKFWVQDCSAATQ
                     NILLAATALGLGSIWVGVHPVTIFSRQVSHILNLPKGVTPLNLIYIGYPAETKEPRTQ
                     YEEARVQWGPFPPAEKKHAKTFPQDQSILLDEQSNDHTDQ"
     gene            69859..71085
                     /locus_tag="PLW25_00750"
     CDS             69859..71085
                     /locus_tag="PLW25_00750"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS53046.1"
                     /translation="MLEHKLSQTFSSLQHPNFRLWFGGQIVSLIGTWMQATAQGYLVY
                     ELTASPMMLGIVGFANGIPVWLFSLFAGTVADHISRRKMLLITQGSMMVLAFILAVLT
                     FSGTVRAWHIILLALLLGTANAFDAPGRQSFVVDLVPKQELTNAIALNSSVFNLGTII
                     GPAAAGLVYAWLGPGWCFTINGLSFIAVLLALALMKIAAKPPLRPAQGSLAHTLTEGV
                     RYSFKDANIRVLLVMLTASAVFGFGLLALMPAWATGVLGGDVRTNGLLLSARGLGSLS
                     AALMIAYLGARIIRGRLWSIGSLVMPVSLLLFALFRLLPVSLLMLVLMGWSLMTVVNL
                     TNALIQTHVPDELRGRVMSVYILLFQGGFPIGSLVAGWLAGLTSEALAVFVFASVLLA
                     VSIAIQLRQPSIRQYN"
     gene            71176..71886
                     /locus_tag="PLW25_00755"
     CDS             71176..71886
                     /locus_tag="PLW25_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HOS53047.1"
                     /translation="MRIEPETSISHDHTSTEGGVQPRRKRIKNRGRLVVNLFAAVVFL
                     ILLAGYLAGWLGEYPARNAQLITGRIAFLFLLASLSITPLRTLTGNIHIGPLRQAFGL
                     NAFYFAMVHVLILIFLNYRMDFYALVKGFSNHPYFWPGAVAFLILSVLAVTSLNKMKR
                     ALRGSWKKIHTMVYPAGVLVLVHYSWIANGKFIPAAEVKTLPLLTAAYLAALFTLRLR
                     PVKKAVLRWRKKAKPRTG"
     gene            71945..72017
                     /locus_tag="PLW25_00760"
     tRNA            71945..72017
                     /locus_tag="PLW25_00760"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71978..71980,aa:Lys,seq:ctt)
     gene            72703..73065
                     /locus_tag="PLW25_00765"
     CDS             72703..73065
                     /locus_tag="PLW25_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOS53048.1"
                     /translation="MTEENNSSVDTSLEAPKPKKALSFFSILSFIAGIGTYVWFFGMV
                     SSKPILTFLLTPFFALIAVITGHKGRHDIRKSLMDMKGKTLANIGLLLGYVIILTGIF
                     IIVLAVMGVVTIAGLLGS"
     gene            73114..73446
                     /locus_tag="PLW25_00770"
     CDS             73114..73446
                     /locus_tag="PLW25_00770"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOS53049.1"
                     /translation="MTLPPRLEIPPAAVTPPSINPKDSPLALTSLITGILGWTILPLL
                     GSIAAIITGHLEKKEIKESHGALTGDGMATTGLILGYIQIGFIVLSLIAVIILLLVLP
                     KNNWGLSL"
     gene            73553..73873
                     /locus_tag="PLW25_00775"
     CDS             73553..73873
                     /locus_tag="PLW25_00775"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOS53050.1"
                     /translation="MTTPPSNYEQGVPVFPIVKRESSLAIISLVAGIIGWTIIPFIGS
                     VIAVITGHLAKKEIRESGNTMSGDGMALAGLILGYTMIGISLLAILVVVLAFLLIARS
                     ELGL"
     assembly_gap    74112..74211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <74212..75327
                     /locus_tag="PLW25_00780"
     CDS             <74212..75327
                     /locus_tag="PLW25_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS53051.1"
                     /translation="RNCFMPAIYPFTAIVGQERMRRALVLNAVDPRIGGVLIRGERGT
                     AKSTAARALAALLPSVKVVAECRFGCDPEKPATWCTECRERAERSEKLEAITRHTPFV
                     NLPVSATEDRVVGTLDIEKAIQKGVRSFEPGVLAAANRGLLYIDEVNLLDDHVVDVLL
                     DAAAMGVNIVEREGISFTHPARFILVGTMNPEEGDLRPQLLDRFALSVEIRGIRDARE
                     RVQIMERNIAFEEDPKLFYDSWEEKEQELSHQIENARKLVDEVTYTRQNLLSIAALTS
                     SLNVDGHRSDLVILKTSRAQAAFDGRRQINDRDIALAAELALPHRLKSGPFQREEMGM
                     EELQERIEQLQGGTSGENEEETISELGGEETGSPKKA"
BASE COUNT        17571 a        20666 c        19131 g        17353 t
ORIGIN      
        1 tcacaacacc ttcttttccg gcgaaaaatg aacgcggatt cttgcgcaaa tagatgggtt
       61 tgggaaagga gtcgcgtatg accacggctt aggagttggc gtcaattttc tttacagaga
      121 tttttcgctt tttcgctggg ggaaggtcac atgaagcgcg ggtgacacct tcctgaaggc
      181 tctcatccat attctaagaa tggaagcccg ccttgcgggt gaatcaccac tgaagtgcag
      241 caagcatgcg ccggccaggc cttgctgccc gcagcccttt cttcctgggt tcagaaaacc
      301 agtgaattgc gtcagcgcat gcacaatcta ataaagacca tttttcgatt tgaagagttg
      361 aaaggtgatt tacgggttcc gtgccatggt gtttggtaat gaaacccctg gtgaaccaaa
      421 aatgacctgg ggagtgggcc cggcagggat cgaacctgcg acaaagcggt tatgagccgc
      481 ctgctctaac cactgagcta cgggccccaa tacccgcatc gattataaca ttaagaaatt
      541 gaaccgtgct cgcaactcat ttcaaggggt tctgcacagc ggaatcccct ttctcaacaa
      601 ttgttgcttt cttcaccata tgctgaggta ctcccctggg ccatccccgt cccttgaccc
      661 ttcatcaagc agcgtttata attcaatgat tgaatattgg agattcatga gttacccatc
      721 cacctctggc cgagacctcg tcatcttaga gcaaatcgaa caggatcccg acaccaccca
      781 ggcagcactc gcttcaagcc ttgaagtggc tgtgggcacg gtgaactggc acctcaaacg
      841 attgatcgaa aaagggtatg tcaaggtcag ccgcctggaa cgcaagaaac tcaagtacat
      901 catcacgcca gaaggcatcg ccctcagggc gcgcctgaca gttgacttta ttcaaaattc
      961 gttcaatctc taccgattgg tgcgtgaacg cgctgcgcac gctatccaaa acctgaaagc
     1021 gctgggttat caccaggtac ggctggaagg cagcggtgaa gtggctgaga tctgccgtct
     1081 gacctgcctg gagcacgaga tcacgctggt tgaaaatgag tcagcacctg tgctcaaact
     1141 tgtggggctg aaagttattc tggaaacgaa ggagcatgag catgtcacct gattactggc
     1201 aggataaacg cgtgtgtgtc accggcgggg ccggttttct tggttcgttc gttaccgcca
     1261 ggttacacga gcgcggagca aaagaggttt tcatccccac cattgaaaaa tatgacctcg
     1321 tgcaactgga ttcgatccgc cagatgctgg atgacagcaa cccggatatc atcattcacc
     1381 tggctgccca ggtaggcggt atcggcgcca accgcgaaca cccggcggag ttcttctaca
     1441 gcaacctgat gatgggcgtg caattgatcc acgaagcctg gctgcgcggt gttgaaaaat
     1501 ttgtcgccct gggaaccatc tgcgcctacc ccaaattcac gcctatccct tttaaagagg
     1561 aaaacctgtg ggatggttac cccgaggaaa ccaatgcgcc gtacggactg gcgaagaaga
     1621 tgctgctggt gcaatcacag gcttaccggc agcagtatgg gttcaattcg attttcatca
     1681 tgccggtcaa cctttatggc ccgcgtgata attttaaacc cgagagctcg cacgtcatcc
     1741 ccgcgctcat taaaaaatgc gttgaagcgg ttgaaagcgg cgcggatttc atcgaagtat
     1801 ggggcgacgg cacacccacg cgtgaattcc tgtatgtaga agacgcagcg gaaggcattt
     1861 tactggcaac cgaaaaatac gattctcctg aaccgattaa cctgggctcg ggcatggaaa
     1921 tcagcatcaa gcacctggtt gaactcatcg ctcatctcac tggtttcaaa gggcgcataa
     1981 tctgggatac cagcaagcct aacggtcaac cgcgccgccg actggatgta acccgcgctg
     2041 aacaggcgtt tggctttaaa gccgggacct catttgaaga aggtctggag aaaaccattg
     2101 agtggtatcg cggtcagaaa gggtattaaa agcacgaatg gcaactgtca acacgcttga
     2161 acagaagaga aagccccctg tggtagtcat ccagccatcc cgcggttggg tggggctcaa
     2221 gctcaaagac ctgtggctct atcgcgaact ggtgtacttt ctcacctggc gggatatcaa
     2281 agtccgctac aaacagtccg tgctcggcat tttgtgggcc attctcaaac cttttatggc
     2341 gatggtggtg tttaccatct tttttgggaa tttcgccaag atcccgtctg acggtgtacc
     2401 ctaccccatc ttctcctaca cggctacctt gccctgggag ctcttcgctg ccgcgttggg
     2461 ggttgccagt cgctcgatgg tttcaaacag caatatggtg tccaagatct attttccacg
     2521 catgatcatc ccgctggctt cggtgatgtc cagcgtggtg gacttcttca tcggtttcac
     2581 tattctcatt ggtatgatga tcttctataa agtaacccct actattgcca ccttgtggct
     2641 gccgctgctc atcctgctgg cgctgattac cgcccttggt gtgggttttt ggtcttcagc
     2701 gcttatggta aggtacagag atgttggtta cattatgccc ttcatcgcca atctttggat
     2761 gtacctcact cccgtggtct atccttccag tatgatcccg gaaaagtggc gactgctcta
     2821 cgcgcttaac cccatgaccg gcgtggttga aggttttcgt tatgccctat tgggcaccac
     2881 gcaatctgtt tctgggggga tgatactggt atcctccctg attgccatcg ccgtgttaat
     2941 tagcggtatg ttctacttcc ggcgtatgga aaagcaattc gccgacatga tttaaaggac
     3001 ctgttaatga gcaacctggc tgtccgcgtt gaagatatag ggaaacaata caatattttt
     3061 caggaaaccg cccgttacat cacgcttcgt gaaaagatcg tcgaaaccct caagactccg
     3121 gtgaagaccc tgcttaaccc taccggcagc gagaagtttt gggcgctcag gcacgtggac
     3181 ttcacccttg accatgggca ggtgctgggg gtcatcggca agaacggcgc cggtaaaagc
     3241 accctgctta aaatcctgtc gcgcgtggtc gaaccaactg aaggtttggc tgagatccac
     3301 ggtcgggtgg gctcgttgct tgaagtagga actgggtttc accctgaact caccggccgc
     3361 gaaaatatct tcctcaacgg cgccatcctt ggtatgaaga aatccgaaat cgagagaaaa
     3421 ttcgacgaga ttgttgcttt ttcagaaatc gagaagttca tcgacacacc ggtaaaacgt
     3481 tattccagcg gcatgtacat gcgcctggct ttcgctgttt cagcccacct tgaaccagag
     3541 atactggtgg tggatgaagt gctcgcggtg ggagatgctg aattccagcg caaatgcctg
     3601 ggtaagatga gcgatgtcgc atcagaaggg cgaaccgtga tttttgtcag ccacaatatg
     3661 tccgccattc agcgcctcac acaggagtgc attatcctgg acaaaggcgc gatggtgctg
     3721 cgcgcgcgca cccccgaagc ggtggattat tacctcacca atgatttttc cgccagcgga
     3781 gaacggcgtt acacggaaga agagtcctct tctgctgcgt atcctttcaa acctgtcgcg
     3841 gtgcgtgtaa aaaatccctc cgggcaggta acagacagcc tgttttcagt agaccctctg
     3901 accattgaag tggagtatca gctcgacgct gacatcaccg gtttgcgtat cggcatctac
     3961 atgatgacct cgcgcggaga attcattttc acatcctttg acaccgatga accggaaaaa
     4021 ttccaatcca tgagcatgcg caaggcgggt cattacatca gccgctgtgt catccccgca
     4081 gataccttca acgaaggacg ttttgtgatc ggtatcaacg ccagctctta tatggttaaa
     4141 cggtatttca gcgatgaacg cgctatgaac tttagtatca acaccactgg cgcacctggt
     4201 atgcactggg tggaaatccg ccacggtcct atccgcccca gacttgactg gagaattgag
     4261 gaaatcgcgt aaatggcgag caacaaagag ctactaaaga aaatcctggg ggagcttcca
     4321 ttgacagccg aggtttacca gttgctgcag caatctgatc gcccgctgcc gaatcatttt
     4381 tcgttgcgca acatccgaaa ggtacttcct gaagcagtgg cggctgtaca aaagtcatgc
     4441 gctcaatacc ctgcgggcaa gaacgtattc atcttcgcat ccacccatta ctggctggag
     4501 catggcgtga taaccggatt ggcgctttca gggctcggtc acgatgtgac catcggctat
     4561 tacccttacg gtcgttggga tttcaattct ccgcagtttg acgtgcggcg ccaaagtgta
     4621 tattcagcca ccgtcttgcg tccagcctcc gcggtgatga atttcgaaaa cctgctacac
     4681 acccattctt cattctcatt gctgcctgag gccctgcaac aggctgttga ccaggtgact
     4741 gacttcgact acatgtacgc gttccagaca gaagaagtga atagggaaga ccctttttac
     4801 ctattcaggc atgagcgcaa tctacacgcg gcgaaatcgt tactcaccta cctgcgttcg
     4861 ttgaagcctg aagttgccat catcccgaat ggcacgatcc tcgaatttgg aatcgcatat
     4921 aaggtatgcg gttacctgaa gattcccacc acaacttacg agttcagcga taaacgccat
     4981 tcgatgtggc tggcgcataa ccaggaaatc atgcagcaga acactgatga gctgtggaaa
     5041 acccacgcca attatcaact caccaacgct gaccttgagg aattaaaggt actgctctct
     5101 tcacggaaaa acgcctccac ttatggtgat ttttctcgcc agtggcagcg ggttcccgct
     5161 gaaggacagg cagccattca ggcgaaactg gggtttgatg gaaagcccat cgtcctgttg
     5221 gctaccaacg tcctcggcga cagccttacc ctggggagga acaccttctc caggtccatg
     5281 tctgagtgga tcattcgcac catccagtat ttcattgaaa aacctgaagt gcaactggtt
     5341 atccgcatcc atccgggtga agccttgatc aagcatggca gtattctgga ccttattcga
     5401 aaaacgctgc caaccttgcc gccgcatatt caccttattg aaccgctggc tgaagtgaac
     5461 acatacgacc tgatggatat tgccgatttg gggttggtat acaccaccac cgttgggctc
     5521 gaaatggcga tgcgcggcat tcctgtcatt gtggcaggta aaacccacta ccgcaaccgc
     5581 ggcttcaccc atgatcccaa cacctgggtg gactacttca agctgctcaa caggctgctg
     5641 acctgtttac gtgaatgccg cctgcaggaa gaccaggtca acctcgcctg gaaatacgct
     5701 tacctgttct ttttcaagta tcccctgccg tttccctggc atatgacgcg cctgaaagaa
     5761 gatctcgagg aatatccatt agaatacgtg ctttcagagc ctggtttaga gaaatttcgt
     5821 tcaacgcttc aactgctgct ctctacaaga taaagaggta gatgatggat gatcaagaat
     5881 atgaaaacct gcttttcagg cggcagtatt ttataggtcc taaagaatta gggatttccg
     5941 gttggaacct tactcacctg ccggatggca aaatcctgag cgctcaccct gaactggaag
     6001 ttactgattg gaatggaaag cacacgcgtt acgttctcat cggctacatt tttgatcctt
     6061 tccatcctga gcgcacaaat caaggcattt tagaggcaat tgaccgctgc aaaaccgata
     6121 ccgggtcaat cttcgagatg cttgaagaca aaagcgggca ttatgtgcta ttcgtttacg
     6181 atgatgaaat gaacattgct ctcactgacg cctgcgggat caagcaaacc tcatactact
     6241 gtgatgagaa tggaaagagt tattattcat cacaaccagc cctgatcgcg caaaaactca
     6301 acctccccgt ttcggatgaa gccattcact atatggactc ggcgaaatat aagaaaaagc
     6361 tggagccctg gtggccggtg gagtcttcac cttataagag aataaggcgt ttggtgccga
     6421 atcactatct gaaccttgcc acaaacgagc aagttcgttt ctggccgaat aagaagctga
     6481 agcgttattc tctgaaagaa ggcacgatcc tggctgggga gctattaaaa ggcatctgta
     6541 aagcctgttt tcaacgctac cccatggcat taacctttac cggaggatac gactcccggg
     6601 ttctcttagc cgcctgtaaa gaattcgctg atcagattga acttttttct atgatttacc
     6661 gcaacctcac agaacaaagt gaagacctgc ggattcccca ggcaatcgcg gcagaaaaac
     6721 aactcaagta tcatcaaatt aaatgcgacg cggaaatacc agccgggttt caagatgtct
     6781 atgagcgcag taatcaaggg tataaaactg attggatgag ccttgtgtat ggcaggtata
     6841 tccattttcc tgccaatgta ttgatcgtca aaggcaacat tgcagaagtg gctcgttgtt
     6901 ctttctggca ggatggcatt taccctgtag aagtaactgt ggatacgctt gtggatgcta
     6961 ccgccttagg acacgaaccc cttattttac aatccatggg aaaatggttg gatgaagcca
     7021 aacccaccga aaagttcgga tataaaatca tggatatttt cgcctgggag gtggaggcag
     7081 gggggtggca gtctatgagc cacaatgttt ttgctttgtc gcaggaagaa ttttctccat
     7141 ttggcaaccg gcgccttctt gatattatgt taggcgtgca caagcgctat cgctgctggc
     7201 ccgatattac cctcgaacag gaaatcatca aatacctctg gcgtgacctt tcacaatact
     7261 cctatttctc ttcgtggaat ttgcataatt acaaaaagaa gttttatgat ggcgacctgt
     7321 tgaatttcct gcgcaagatt aaacttatac tgaagaggag cattaaatga attggtctga
     7381 cagtgtcgtt ctggtaaccg gagggaccgg ttcgttcggt aagaaattta tcgatatcat
     7441 gctcaaggaa tatcatccac gcaggctggt cgttttcagc cgggatgaat tgaagcaatt
     7501 tgaaatgcag gcggccgggt tttcacaccc ttcgctgcgt tacttcattg gagatatccg
     7561 cgacctgcag cgttgccggc gcgcctttga gggtgtggac ctggtggtgc acgctgccgc
     7621 gctcaaacag gtgcccgcct gcgaatacaa ccccatggaa gccattaaga ccaatatcct
     7681 tgggagcagt aatgtcatcg atgccgccat cgacgctgga gtcagccggg ttgtggcgtt
     7741 gagcacggac aaagccgtca accccatcaa cctttacggc gcgaccaaac tggctgcgga
     7801 aaaattattt gtgcaatcca atgcatatgc tggtgccaga aaaacacgtt ttgcctgtgt
     7861 gcgttatgga aatgtcgtcg gcagccgcgg ctcggtggtg ccgtttttcc tcaaacaacg
     7921 cgagactggc agggtgacgg tcaccgatga acgtatgacg cgcttttgga tcagcctgga
     7981 gcagggtgtg cgatttgtca tccgcgcggt tgaaaacatg cacggcgggg aagtgtttgt
     8041 tcccaagatc cccagcatga cggtcaccga cctggctcgt gccatcgctc ccgaggctgc
     8101 gattgatttc attggtatcc gcccgggtga gaaactgcat gaagttctca tctcagaaga
     8161 tgaagcgcgc cagacggttg aactggaaga tatgttcgtg gtgcaaccgg cggagtctct
     8221 ctggttcggc agggattggg agggcaaagg acgtatgctg gaagatggct tccgatactc
     8281 ttccaataac aacgatcagt ggttgaacgt ggaacaaatt caaagcatta tcgcccccat
     8341 cgaagccgat tacctggcag gcaggctggg atgacgcgct tcctggttac cggagcaagc
     8401 ggtttattag ggctgaattt tgccctggcg gtggatggca aaaaacacca ggtcaccggt
     8461 gttgccaaca cagccccgat gttgtgggca acttttaaaa acgtccagct cgagctcacc
     8521 caacccggtg tggtggatga gatgataaag gagcatcaac ctgaagtagt gattcattgc
     8581 gccgccatgg cgaatgtgga cgcctgtgaa aataaccccg cccaggcacg gctggtgaac
     8641 gctgaactac ccggcacgat cgctgcagcc tgtaaacgcc acagggtgcg cctggttcac
     8701 atttccaccg acgcggtttt tgacggtaag aaaggggact atagagaaga agaacccccc
     8761 catccgctga gcgtttacgc cagcaccaag ctggagggtg aacgagcggt actcgctgcc
     8821 aatcccgatg cgctcgtggc gcgcgtcaac ttctacggtt ggagcgccgc gggcaatcgc
     8881 agcctggcag agaatttcgt gcgcaacctg gaagcaggca aggaaatgat gggctttaca
     8941 gatgtcatct tctgcccaat gaatatcctc gatctcagca gtgtgctggt ggaggctgcc
     9001 gaactggata tcaggggtgt atatcacatg gttggcgcgc aggcgatgag caaattccag
     9061 tttggcgtgg ttctggcaga gcaattcggc tttgacacca ggctcatcaa acctgtctct
     9121 gtaaatgaca gcggtcttaa agcagcgcgt tcgcccaacc tgagcctctc aacggaaaaa
     9181 atcgtaaaag ccctggggca tgaattacca gattttcacg aaggcgtgaa gaaatttttt
     9241 gaccagtacc gccgcgggta tccgcaatac atcatgtcac tgaacggcta acaggtaggc
     9301 gaaaggagca aaaatggaat tgaaaattgg tcagcatttt atcgggttgc agcaccctac
     9361 ctacttcatc gcagacattt cagccaacca cgatggcagc ctggagcgag ccaagatgct
     9421 catcaggctc gccaaagaag ctggcgctga tgctgccaag ttccagaact tccgcgcccc
     9481 aaaaatcgtg tcagattacg gctttaaagc catgcagggg caggtctctc atcaggctac
     9541 ctggaagaaa tccgttttcg aagtctactc agacgcctcc atcccttttg actggactcc
     9601 catcctcaaa gaggagtgcg ataagttaaa tattgattac ttctcctctc cctatgattt
     9661 tgaagcggtt gattatctcg acccgttcgt gccggcgcac aaaattggtt ccggcgacat
     9721 tgactggctg gaaatgctgg aacacattgc cctcaaagga aaacctgtca ttctttccag
     9781 cggtgccgcc accatcggcg aggtgcagca agcggtacac accatcttac cgcttaacca
     9841 gcagttggtc ttgctgcaat gcaataccaa ttacactgcc agcccggtga attttgacca
     9901 tatacatttg cgcgtattac aaacctatca gacgctcttc ccggaaattc ctgtcgggct
     9961 gtcagaccac acgcacggtc acgctactgt cctcggcgca gtggcgctgg gggcgagggt
    10021 gatcgaaaaa catttcactg atgacaccag ccgtgtaggg cctgatcacc ccttctcgat
    10081 gactcccgtt acctggcgtg agatggtgga tcgcacacgc gaactggaac gcgcgcttgg
    10141 ctcagctgat aaatcggtca gcggtaacga ggtagacact accattgtgc agcgccgctg
    10201 cctgcgcgcc tctcgtgata ttcgctccgg tgaggttttc acccgcgaga tgatcgatgt
    10261 gctgcgcccg gctactcccg gcgccatccg ccctgaccag gtttttcagg tggtcggaac
    10321 ccgcgcactc aaagacattc ccatgggaaa agagttgcgc tggacggatt tgggagagta
    10381 atttgaaagt tgtttatttc acccgcgatt attcacctca tgacgaacgc tttctcaccg
    10441 cgctcgcgca aacgcaacac gaggtgtatt tcctgcggtt gtcgccctca agcgaagtcc
    10501 atttaccacc cggaatacgc gaagtgaaat gggtcggccc cctcgccggt gaaataattt
    10561 ctgatgacag gcgcgcggaa gatctgaatc agcttctttc caggctgcag cctgacgtgc
    10621 tgcacgccgg tccactgcac gggccggcat atatcgccgg tttaagtggc tttaccccgc
    10681 tggtgagcat gtcctggggt tcagacatcc tgcatgatgg agaagttgac gccctcgccc
    10741 gggcaaaaat caacctttca ctgcatcgtt ccacggtttt cctgggcgac tgtcaggcgg
    10801 tggtagaaaa agcgatcaac gctttcgcgt ttcccgcgga gcgggtattc cggttcccgt
    10861 ggggggtaga cctggcgcac ttttcaccct caacaaccag cccgctgcgc gcatcattag
    10921 gctggcagca gcaattcgtc ttcctcagca accgctcctt cgaagagata tatggtgtag
    10981 acgtggtcat gcgcgccttc atccaggcgc aatctcgcgc cccaaatatc cgcctgttat
    11041 tgtatgggaa gggcagcctt caacaagaaa tcatgcgcat ggctgaaaac gcgggagtga
    11101 tgcacaagat ccatttcgct gcctttgccg ggcatgaaga tctgccagcc agttaccacg
    11161 ccgcggatgt attcctcagc gcgtctcatt gtgacggttc ctctgtttca ctgatggaag
    11221 cgttggcctg cggcaaaccc gcgctggttt ccaacatccc cggtaacctc gaatgggtgc
    11281 accacggcga aaatggctgg ctttttcaag acggtgatac tacagggctg gcagacgcca
    11341 tggtagcggt cagccgcaag ccgaatcttg aaaagttggg tcaaaaatcc cgggcgctgg
    11401 ccgaagaaaa agctgactgg tcgtgtaatt tcgccgtgtt attgcaggct taccaaaaag
    11461 ccgtggaatg gcgtacgccc gccctggaga ttacggagcg ggtgaaatga aagtggtggc
    11521 catcatccag gcgcgcatga gttccacccg cctgcccggc aaagttttaa agctggcttc
    11581 aggtcgcaca atgctggacc gcatgcttga acgcgttaaa aaagcgcgaa cgatcagcga
    11641 ggtggtaaca gccaccacaa ctgatccttc cgatgatagt attgctcgcg cttgtaaaaa
    11701 actgggggtg agtgtgttcc gcggcagcct gcaggatgtg ctcgatcggt tttaccaggc
    11761 atcgcgcatg catcaagcag atatcatcgt gcgcctcacc ggggattgcc cgttgatcga
    11821 ccccgcgctc atcgatgacg tggtgaacgc tctcacccgg aaaaaggtcg attttgcctg
    11881 caaccgtctt ccgccgccct tttcacgcac ctaccccatc ggactggatg tggaagcctg
    11941 tactttcaac gcgctggagc aggcctggta tggagcgaaa gagaagttcg aacgcgagca
    12001 tgtgctgcct tacctttacg ctgtgcctgg caggttttcc gtgcttcaat tgaatcacac
    12061 cacagacctc ggtcacttgc gctggacatt agatacgccc gaagacctgg tactgctgag
    12121 gcgtatctac cggtattttc aaggccgcaa tgacttttca tggttgcagg tcttacaatt
    12181 acagcagcaa aaacccagcc tgttcgccat caacgcgcag gtgcgccaca aaacctacct
    12241 ggaaaatgaa tccccgagat gaccattaaa gaaatgtcag aaaaaatcgt ctgtccccga
    12301 tgccaatcct cttttgataa gcacgatcct gctgtttgga gctgctcttc ctgcgcttac
    12361 acagtgccat tatttcaagg aaagccggtg ttctccgggc atccgcaaga gcagcagccg
    12421 caagacaaga tagagcgcgg cgcagacaaa ggcacagcat ggcgccgcgc caattggcgc
    12481 tttattcatg ctattgcggc atctcttccc gcaggggctg acgtgctgga tgttggcgcc
    12541 ggtcgcggtg attttcgaca aatcttcgcc catcactcat acatcgggtt ggatatttac
    12601 ccttacccgg aaattgacct ggcggtggat ctgatcgaga ttaacccatt caaagaacgc
    12661 tcgttcgacc tggttgtgtt ggcgaatgtc attgagcatg tatacgaata tcgcaaactg
    12721 atcagccgct gcgcgttcct gctcaagccc ggtggtcagt tgctgataac agtgcccttc
    12781 ctgcttaagc tgcaccagga accggtggat taccatcgtt acacccacta tgccttattc
    12841 cagttagcag aagaaaacca actcagtgtg gcgcgttttg aggtttatac caatccattc
    12901 gcgctgctgg acgaagggat tgctgatgtg tgggagaacg tgcttccgct gcgccaaaga
    12961 attgaccacc tgcacaccaa ggtgaaagta gcgcttattc aaaagctggc gaacagcatg
    13021 aaagccaatc aggcggatgg tgtggtgaaa cccgtccaag agaacagcaa tccccgggtg
    13081 ctggggtata tgtgtctgtt tcaaaaggat gcccttcaat gacaagcgtg gcttttttca
    13141 ccgctcccaa accattcaca aacgcgcata tccgcatcat ccagcgcaac gccattcaaa
    13201 gctggaaatc actgggcagg caggtcgaag tgtggctggt gggggatgaa ccaggtgtgg
    13261 agcagaacgc gcgcgagttg caggtcggtt atatcccggg agttgcgagg aacagcttcg
    13321 gcacgccgcg catcgattcc atctttcacc tggttcgtga acaatcgcag gcagaatacc
    13381 tgtgctatgt caacgcggat atcctggttt tcccggatat gttacgcgct ctcgaagagg
    13441 tgcgcgcgcg cgtcgatcgt ttcctcatca tcggcaggcg ctgggacgcc gcagtgacag
    13501 aaacactcga cataaaagaa ggctggcatc aacagttcca ggctgccgcg ctagtgaccg
    13561 caaaactgca ccgcgccgcg ggcagtgatt attttttatt cccgcgcaac gaattcatcg
    13621 atatcccggg tttcgcggtt gggcgcgccg gttgggacaa ctggatgatc tacaaagccc
    13681 gatacgaaca tatggcagtg gtcgatgcca ctggcgccat caaagtcatc caccagaatc
    13741 atgatttcag ccattttgcc gacggcaaga ttcaccgcct tcagccggaa tccgctgaaa
    13801 atatgcggct ggcaggcggt cgttacacgg tcttcaccat tcacgacgcc aattaccagt
    13861 tagacaacga cggtctgcaa cgcgtacgcc taaatcgctg gaaattgctg cgagaattcg
    13921 tcattttccc agccgttacg ctgcactgct cctggctggc gaaagtcgtt tatgccatct
    13981 tcaacccgaa acgtttgctt aaagacaggc gccaggcaaa agaagcgctg cgccgctcaa
    14041 aggaagggta aatgagaaaa ggtcaaaacc cggtaaaaca acttcagacg gtgtctaaac
    14101 cgcagcgggt gaccgtggcg gtgttgaacc acatcccttt tctggatggc ttttacgcgc
    14161 agatgctcga tgttttacag ctcagcttga gcacactgcg ccagaacgca ggcttggatt
    14221 ttgacctgct tgtttacgat aacggctctt gcaagcaggc tcgcgacttt ttactcgcag
    14281 aatacgaaaa aggaaatatc cagtcactga tcctctcaga ccgcaacatg ggcaagggcg
    14341 gcgcctggaa cgtgatgctg caagcggcgc cgggtgagat catcgcttac gcagacaacg
    14401 acgttttcta tcatcccggg tggctgcgcg aatctgtgaa agtgctcgaa acttatccca
    14461 atgtgggtat ggtcacctcg cggccgtttc ataccaaacc agaattgtac agcgctgacc
    14521 tcgattgggc gcgtaaaaca cctgaagcgt cagtagaaag aggttttttt atcgaccgtg
    14581 actggatgcg cgagttctgg ctcagcgtag gtcgcagcga ggaggagatc gccctagacc
    14641 aggcggttga agatgtgcgc ctcacgtata aaggtcttgt cacctactgc ggggcttccc
    14701 actggcaatt cctggcctgg aagaaggtcc ttcaggaatt tcttcctatt gatctatcgc
    14761 gcccgctggg gcaggttctt aagctggatg agatgattaa ccagcaagga tacctgaggt
    14821 tgatgaccgc ccagccgttc accatgaact tgagcaattc agtcgaactg ccctcgaatg
    14881 aaccgacaag cgcagcagct caaccccgta cgcgcaaacg gctggcagat tttcctgtgg
    14941 tcaaatcccc gctgatgcgg ttgtacaacg ccatattcaa gctttattat gacgataaga
    15001 agcgttgagg agaaaaaatt gaaaatctta tacgcgacgg gaagatacag cccactcaaa
    15061 cacgacgatg gcagcggtac agattaccgg ctgtatcacg cgctattaag ccagggcgta
    15121 gagatgaaat tcatcggccc attccctgat cacccgtcgc tgatagagcg cctttaccgc
    15181 aaggtgcaca ccgtgttttc gcataagcga ccggcgaaat ataccgccgc aatgctcaaa
    15241 acagccgcgc aaacattatc tatcactgtt aatgaatacc aaccagacgt gatcttttca
    15301 aaaaaccttg ccccactcgt gcgtttccat gcccaactcc cagtggtgta catgctggat
    15361 tccactgtgg cgggatttaa ccgaggctgg ccaacttttt ctcgtttgga aaactggcgc
    15421 atggttgaat gggaaaagaa agtgcttaag caatccagca gcgtcatcac ccgcagtgag
    15481 tggacaaggc aatcgctaat agaagattat cactatccgg ctgaaagaat tcatatcgtt
    15541 cacaatggct cttcccttcc tgatcatgtt attccacaag agattgagaa cgaaccgccc
    15601 gatttttcca ccatacgatt gctctttgtc ggacgggtga tgcatctgaa aggaatcgat
    15661 atcgcgattg aaatcgtcaa ccagttgaac gaatgtggca ggcaggctga cctcagaatt
    15721 gtcgggcttg atgggcagga ccaagaccac atccactaca tggggtcatt taaaaaaagc
    15781 attgaagatc agttaatcgc ctacgcaaac cagtaccgat ggccgcattt ccttttacat
    15841 cccgcccggt atgatgcggc ccccattgtt accgcagaag ctgccgcgtt tggcgtgcct
    15901 acccttacca ataatgtggg gggtatcgcc tccatggttc aagatggtct gtctggggtc
    15961 gtgctccctg ctttaagtcc ggccgcagaa tatgtaaaga aaattgtgta ttacgctgac
    16021 cacccgcagg aatacatggc actgcgccgc actacgcgcg aacgatacga aaaagaactc
    16081 aactggaatg tagctggtaa aaagatagtt accatccttc aaagtgtggt ggaagaaaat
    16141 cagttgaaac ccgggctgaa gaaagagcaa ccatgaaaat cttaattgcg ggttatggtt
    16201 cgattggcag acgccacctc aacaatctgc gcgcgctggg tcaaactgac cttttattgc
    16261 tccgctctca ccgctccacg ctctccgacg ttgatataca ggatgtgccc gtggaaacca
    16321 ccatcgaagc cgcgttggcg caccagccgc gggcggtggt catcgccaac cccactgccc
    16381 tgcacctgga tgtagccatt ccagcggcgc aggctggctg ttccatcttg atggaaaaac
    16441 ctgtttcgca ttcaatgagc cgggtagagg aacttcgccg cgcgctcact agcgggggcg
    16501 gggttctgct cagcgctttt caataccgtt tccaccccgg gctgcgccag gtcaaacgct
    16561 ggctgcaaga agaccagatc ggcgtcatct cctcggtcaa gtcgcattgg ggcgagtacc
    16621 tgccaggctg gcacccttgg gaagattatc gcagcagtta cagcgcccgg gtggaccttg
    16681 gcggtggagt ggtaaacacc ctgtgccacc cgtttgacta ccttcgatgg atgttcggcg
    16741 aagcagaaca gttagttgct tctacctcca gccgggggtt gaacctgccg gttgaagaca
    16801 cagcagatgt gctgcttcgc taccagggtg gtgtttgtgc caatatacat ttagattatt
    16861 tgcagagacc cggtcaacat gacctgctta tcaccggcag cgccggcacc atacgttggt
    16921 caaacacgga tggcagcgcg aaaagatatg accccatcca tgaccagtgg gaagaggtgc
    16981 gcccgccaga aggttttgag cgcaacaccc tctttttaga agaaatggcg catttcctgc
    17041 gcgtggtgga aaggcaggaa gcaccactgt gtacactgga agacggaatt gccgtgctac
    17101 aactaacgga agcagtgcac cgctctgccc gtgaaggatg tctggtaaaa ttttagcggc
    17161 ctattggagg ttgaatgacc agtatttttg aaaaattttc attgaaaggc agggttgcgc
    17221 tggtgactgg cggcgccggc cttttaggaa aagagttctg cctaaccctc gcccaggctg
    17281 gcgcgcaggt gtacgtcgca gacctcaatc tgagcgcggc tgaaagcgcg gcgcaaacca
    17341 tcaccgccca aggtcattct gcctgcggca tcaggctgga cgtcaccaac ccggattcag
    17401 tgcagtctgt ggtcgatgaa atcgtttccc gctccggcag cattgatgtg ctggtgaaca
    17461 gcgccgcaat ggaccccaag ttcgaccctg gtcacgcgca aaccaacagc aacgcttttg
    17521 aaacatatcc tctggggtca tggcaagatg cgctcaatgt caacctcacc gggccattcc
    17581 tggtttctca ggcagtttgc cgccaaatgg tgcggcaggg taaaggttct atcatccata
    17641 tttgctcaac atacgggttg ggcgggccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17761 nnnnnnnnaa agacgagatg aatggcgcgc tgctcttttt agcctctgac gcctcctctt
    17821 atatgacggg tggaaacgtg gtggtggatg gaggatggac agcttggtaa aaaaaccgga
    17881 ggtattagcg cttattcctg cgcgcggcgg ctcaaaaggc atcccgcgca agaacatcaa
    17941 actgttcgcg ggttttcccc ttatcagtta cagcattgct gctggccggc aggcgcagag
    18001 cgtcacacgg gtcattgtgt ctaccgacga tgaagaaatt gctgctgaag cgcgccgttg
    18061 ggaagccgaa acgccctttt tgcgcccgcc cgaactggcg caagatcaaa ccactgacct
    18121 gcctgtcttt cagcacgcgc tgcgatggct tcgtgaaaac gaaggctata ctcccgacct
    18181 ggtcatccaa ttgcgtccca cctcacctat ccgtccgcgt ctttgtgtgg atgaggctgt
    18241 ggcgcttttg agaaatcatc caggcgcaga tagtctgcgc ggagtggtgc ccagcggaca
    18301 aaacccctat aaaatgtggc ggttgcaggg caaagacgaa ccgatgaccc ccttgctgaa
    18361 actggatgga atcgctgaac catttaacgc gccccgccaa tcgctgccgc ctacttactg
    18421 gcaaaccggg catattgatg tgatccgccc tcgggtcatc cttgagggca attcgatgag
    18481 cggcagaaat attctgccct acctcattga ccctcggtac acggtggata tcgatactcc
    18541 ctttgactgg ctgcgttacg aatggctggt gcgcaacgct gacctggata tggttgaccc
    18601 ggccagcaag cggcgcaaac tccccgcgaa gatctcgctg gtggcaatgg attttgacgg
    18661 tgtgctcacc gataacctgg tctatgtgga tcaggatggc agcgagcgcg tcgcttcatc
    18721 gcgcggtgat agtatgggca tccgcctggt gcgcgaagcc ctgccggtaa attttgtggt
    18781 catctccacc gagaccaacc cggtggtcag cgcgcgctgc cgcaagctaa acctggaggt
    18841 ctaccagggg gtcaatgaca aacccgcggt cctcaaacag ctgctgcaag aacgccaggt
    18901 tcctgctggg gaggtcgtct tcatcggcaa tgatgtaaat gatgtcggtt gctttgacct
    18961 tgctgcttac gcggtcgccc cggctgacgc cgagccagaa gttcgacgcc gcgccgacct
    19021 tgtgctcacc cgtaaaggcg gaaaaggcgc ggtgcgggag ttttgtgacc ttcttttaca
    19081 gaatgctggg aaaattttgc ctacacagga gaaaaaatga cacgagaagt caagattggc
    19141 aataaatggg tcggcgacgg ccaccccgtc tacatcgtgg ctgaagttgg catcaatcat
    19201 aacggtgata ttgaaatcgc caaagaactt atccgcgcgg ccaagcacgc cggcgtggat
    19261 gctgtaaaat tccaaaaacg tacccctgaa ctatgcgttc cacctgaaca acgcggacag
    19321 atgcgtgaaa ccccttgggg ctacatctct tacctggatt accgctacaa agtggaattc
    19381 aacgaggctg attatgatga aatcaaccgc tacgcgcgtg aaattggcat cgaatggttc
    19441 gcgtccgttt gggatatccc ttccgttgac ttcctggaaa agtacgagcc catctgttac
    19501 aaaatacctt ccgcgtcgct gaccgacgtg gaactgctgc tgcatctcag aaagaccagt
    19561 cgtccgctca ttctctccac gggtatgtct acccaacaac agatcaaaga cgcggttaag
    19621 cggataggcg aagaaaacct ggtcatcact cacgccacca gcgcctaccc ttgcgacccg
    19681 tatgaattaa acctccggat gatccaaacg ctgcgcggta tcttcaattg ccctatcggc
    19741 tactctggtc acgaagtcgg actggtgacc tctgcagtgg cggtgggttt gggggcctgt
    19801 atggttgagc gccacatcac gcttgaccgt gcgctgtggg gatctgacca ggcggcatca
    19861 gtggagccgg gcggttttga aaaactggtc aagtatatcc gtgtcaccga agcagccata
    19921 ggcgacggcg tgaaaaaggt gtacgaaagt gagatgccct cgctgcgcaa gttaaggcgg
    19981 atccaggatt gaataaagcc tttttgcggg tgagaaacgg tatcaggcag cgctgggtgc
    20041 ataaaaaagc caaccagcgc tggcgggctt tagccacagc catcaccctg caatcgccac
    20101 tgcccagcga cccgcgacca gtgatatttt ttaacgcttc cacccggctg caggcgatga
    20161 gccaaaatgc cgcttatgcg ctgctcaccg cgcaggcgtt gcgcctgcag ggtgttcctg
    20221 tcatccagtt cgtgtgcggc ggggcgttgc agcgttgtgt gttgggcagc cagcaggata
    20281 atttcaacct gccgccgcca tgccaggcct gcacacgcca aagccaggca gttttccagg
    20341 agttgaatac ccgttggatg gaaacgcgtg aacaccccat gcttggaaaa gagctcgaaa
    20401 ccctatcctt caatgagtat gaaaccttca cctatcaagg aactccgctg ggtgcctggg
    20461 cggtcaattc tctgcgttgg atgatgcgcc gccatcacct gcaggaggat gaaacgacgc
    20521 gcgctttctt ccgctctttt atcctctcca gttggaatgt attcactcag ttctctgagt
    20581 tactggatga aacccaaccc cgggcggtgg tgctgtttaa tggcatgttt tttccagaag
    20641 ccgcggcgcg ccacgcctgc ctggaacggg gggtgcgcgt gatcactcat gaggtcgggc
    20701 tcaggccctt tacggctttc ttcaccaccg gcgaagccac ggcttacccg atgatcatcg
    20761 accccgcctt tcagctcgat gatcagatgg agaagaaact cgataggtat ctctctacac
    20821 gttttcgcgg tgagttcacc atggcaggca ttcggttctg gcctgaaatg aaagcccttg
    20881 aagctgattt actcgaaaaa atcaatcact attccaaact cgtggcggtg ttcaccaatg
    20941 tgattttcga taccagccag gtgcacgcca ataccctttt tgagcatatg ttcacctggc
    21001 ttgagcttgt aaaaacaact gctctttccc atcctgaggt gctgttcgtc attcgcgctc
    21061 accctgacga atgccgacag ggaaaagaaa gccgtgaaag cgttgccacg tggttctctt
    21121 catctgaaat ttcccgtctc gataatgtgg tgttcattgg ggccgatgag tacctcaatt
    21181 catacgacct gatccgccgc gcgcatctgg tgatggttta caactcaacc atcgggctgg
    21241 aagccgcgct gctgggaaaa cctgtgctcg ccgggggcaa ggcgcgcttc acacagcttg
    21301 aaaccgcctt ctacccgcag tctgcgcagg aatacaccgt gatgcttgag cggcttctgg
    21361 caacgccgag cattgacaca ccacctgagt tcgcccgtaa tgcccggcgc tttttgtatt
    21421 accagcttta cgccacttcc cttgacttca gtgactacct cgaggaagac ggcgtgtgga
    21481 aaggttacgt taaactccgc gagttctcac ctgacctgct taagccagac aattctctta
    21541 caatgaaggt gctgttaaat ggcatccttg agaatggaga ttttactctg ccgctatgac
    21601 cactccagtt tgttcgatta tcatacgcgc ctataacgag gaaaaacata tcggccgcct
    21661 gctgcgggga atccaggagc aggacctcca tgatgtgcag gtgatcctgg tggattcagg
    21721 ctcgacggat ggtactcgcg acgttgcccg cgcctacccg gtagagatcg tagagattga
    21781 cccgcgtgaa tttactttcg gtcgttcact caacctgggc atcgccgccg ccagcgcgcc
    21841 cctcatcgtg atggccagtg cccatgtcta tccagtctac cccgattggt tgacaaaaat
    21901 gctgcagcca ttccagcaca ctgaagttgg cttggtttat ggcaagcaac gcggcacagg
    21961 cacaacggct ttctctgagc atcaaatctt cagccactgg ttccctgatg tctcagtcaa
    22021 gaaacaatcg catcctttct gcaataatgc caacgcggct attcgaaaag agttatggca
    22081 acagcacccg tatgatgaaa ccctcccagg gttggaagac ctggagtggg gaaaatgggc
    22141 gcaggagcag ggttatacca tctcttacgt cgccgaggct gaaatcatcc atgtgcacaa
    22201 cgagtcgctc gacggtattt acaatcgtta taaacgcgaa gcgatggcgt ttaaacgtat
    22261 ctacccgcac gaaactttca cgctgcgcga tttgaaccgc ctttttttac aaaatgtaat
    22321 cagcgactgg cgcgaagccc ggaggcaaaa ggtcttcgct caccattttt tccatatcat
    22381 cggattccgc tggcggcagt tctacggaac ctaccagggg taccgccagt ctggcccgct
    22441 cacctggcag ttgaagaggg cgttttacta cccccgagat ttccgccagg catccgggca
    22501 aaccagccgc cgagatatcg agccaattaa gtataattag gcaggttgat agaaggtact
    22561 tgcaggagga taaaatggcc catgtaacta ttgagtttac cggtctttca aagtcaatta
    22621 caggggtaaa cgaggtaaat ttgccgatca accccacaga tacctaccgc gacgtggtac
    22681 gccagctggg aacgctttat cccgcattgg tagggatact catcgctgaa gatggcgaga
    22741 ctttcctgag ttccaacatg ttcatcatcg atggcgatta cgccaatccc gctatggtga
    22801 tggagaactc tcccagggac ggtgaacaca ttcacctcat gtctgtcatc accggtggct
    22861 gaaagaacca agaaagtcga ggtttgggtg cctgagaaac tgcaacaccc caagatctcc
    22921 gctctggtgc cgatgcggca tcactctcaa cgtgtgcctg ggaaaaacta ccgccctttg
    22981 aacggcattc ctctctacca gcacatcctt accaccttaa cgcaggtgcc agaagtggat
    23041 gaaattgtcg tggatactga cagcgacatt attctgcacg ggatccagtc aaacttccct
    23101 gctgtcaagg tgatcaatcg cccgccagaa ctacgagcgg atgatatttc gatgaacacc
    23161 atcctgttgc acgatatcag ccagtcacca gccgacctct acctgcaaac gcattccacc
    23221 aacccgctgc tcaaagcctc cacaatctcg cgggcgatca acaagctctt acaggtctac
    23281 ccggaacacg actcgttgtt ttcagtaacc cgcctgcaaa cccggttgta cgaccatcta
    23341 gggaaggcga ttaatcacga ccccaaagtg ctcatccaaa cacaagattt accgccagtt
    23401 tacgaggaaa attcctgtat ctatctgttt accgctgcca ccctgcgtaa gaacggcaca
    23461 cgtatcggag agacccctct actgtttgag acagatccag aagaatcctg ggatatcgac
    23521 gaagaggttg attttaaatt agtcgaaatg atactgaaag cgcgctaacg aatgaaacgc
    23581 ttttcctcgt tttcagtgtt gttgcagctg gggatcttcc tcctgggggt aagcggtagc
    23641 atttacgtgg cttttactcc cgcaaacagc atgatgaact ggtacagtaa tgatgatgcc
    23701 ttttattact ataaagttgc gcaaaatgtg ctcagcgggc atggtttcag ttttgacggt
    23761 atcaacctta ccaacgggtt tcacccgttg tggatggtca tttgcctggg cgttttctgg
    23821 ctctccagat tccatctgct actgccgcta agggtattaa tcgtcatcag cggggttctc
    23881 aacgggctca ccggcgttgt gttgttgcgc ttgcttcaaa agtttttacc gcttgtcgct
    23941 gccatattag gcgcatgcgt ttggattctc ctcccttcga tttacaataa ttacactgcc
    24001 cacggccttg aatcagcgtt gagcgctttc ttcgttgctg tgttgttgct aaaaagcgct
    24061 gacttgctga ccggtcccgc agaaaaacgc gccggaaaat tgatcatact gggtatcatt
    24121 gccgcgctca ccatcctcag ccgcctggac acgcttttcg tggtcgccat ggtggggttt
    24181 tttgtggttt ttaagatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt
    24301 tttaccgacc ttccagccag gtgctcagtg tgtctgcctg gcagcaagta aaaaattgga
    24361 tccggcaagg actgccccat cacctccgtg acggagtgct cttctccttc cccattgtgc
    24421 tgctggtctg cggttacatg ttaaccaata agctggaatt tggtaccttt ataccggtga
    24481 gtggccaggt caagacctgg tggggaacgc tggaaaacac cgtctaccgt cagaacacga
    24541 ccctggtcgg attgatgggt ttggcgccag gcagagggaa aagcccatgg tcgctgctca
    24601 cctcgatcgt tgcggatatt tcgattttca ttaggaacct gttcgtaaaa gatacggaag
    24661 acctgccggt catcctgttt cttgttttcc tttttatgct gttcattttg ctgatctttt
    24721 tgctttcccg caaggatggt ctgctggcca gacgctcctt cgcccttctg attcccgcgt
    24781 tggcgggcgg ctgtcttttg cacattgctt attatgccgc gcgcaattac gggcatacgc
    24841 gcagctggta ctgggtgcct gagacgatgc tcctcgtgct gttgggagcc gtggtgagtt
    24901 ccaggatgtt tgaaaaaatc cggcagtgga cacgaacacc cgtggttgaa aacctgctgc
    24961 tgctggttat ggttggcttt gtcattttct tgcacgcacg atatatcgcc aggctctgcc
    25021 cgccttccgt ttctccagag caccaggcag actatctggc agaaacccgt gaattggaat
    25081 cctatacccg cgaaaactcc ttgattggta tgacaggtgg tggaacaacc gcgtatttca
    25141 ttcaacatcg cactatcatc aacctggatg gtttgattaa cagtgccgaa tacttcaaca
    25201 cattaaaaac cggaaccacc aaccgctttc taaataacct gcacctggat tatgttttcg
    25261 gtaattcgta catgctactc gaaacagatc cttaccagca aatttttacc gggcgtataa
    25321 gggaaatcgg caacattcac agcgggtcgc acttcacgtt atatgattac atcggcgacc
    25381 agtgaggtcg ccgcgttgag aatatatttt gaaggttata ataaccatta ctctatttaa
    25441 ttgaaagaaa acccttatgc cagtcctctt attatctgcc ccttatatgc ttcctttcgt
    25501 caatcgcttt aaaccggttt ttgatcatta ccagctggaa ctgctcctgc cacaggtgaa
    25561 tgaacgcctc gacgaagaag ccctgctgtc tctcgccggt cagtttgacg gaatcatttg
    25621 tggtgatgac cgttttactc cacgggtgct ggaagcctgt tcaccccggt taaaggtaat
    25681 ttccaaatgg ggtaccggta ctgattcgat tgaccgagcc gcttgcgagc gcctgggtat
    25741 ccagctaaag aacacgccaa acgcgttcac aattcctgta gcggacagcg tgttggggta
    25801 catcctggct ttcgcccgcc gccaaccctg gatggaccgg gcgatgaaaa gcgggaaatg
    25861 gcagaagata cctggtcgcg ctttatcaga aacgaccctg ggggtggttg gcgtgggaaa
    25921 tattggcaag gcggtcctca aacgcgccag cgctttcggg atgcgcctgc tgggaaacga
    25981 catcatcccc atccccgctg actttctgca agaatatcat gttgagagtg tatccctgcc
    26041 tgaactggtc tctcaagctg attttatcag tttgaactgc gaccttaatc ccacctcgcg
    26101 tcacctcatc cacaaggata ttatcaggct gatgaagcct gacgccgttc tgatcaacac
    26161 cgccagggga ccggtcgtta acgaaaaaga cctgattgaa gccctgcagc aaaaaagaat
    26221 tgctggcgcc ggactagatg tgtttgaaga agaacccctt cccgcagatt ccgcgttgct
    26281 acatatggag aacgtcctgc tggccccgca taattctaat tccagcccca gagcctggga
    26341 aaacgtccac tggaacagta ttcgaaattt actcatcggt cttgccatca acgcagaaga
    26401 cctggacgag tggaaatcga aagtataaaa ggtgtaccag aaatgaaaga aaacaagcgt
    26461 gttgtgctga tcactggcag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 ttaattgaaa caaccgtcga agaatgggac aaggtgattg ccactaattt gcgcccggtc
    26641 ttcctttcag cgcgtgaatt tcacccgctc ttaatcaacg cgggcagcag cgccattgtc
    26701 aatatgtcat ccgtgcacgc cattcaaact tctgccagta tctcggctta cgctgccagc
    26761 aaaggaggga tgctggcgct gacgcgggcg atggctatcg aattcgccaa agacaatatc
    26821 cgcgtcaacg ccgtgctgcc tggggctgtg gatactccca tgctacgcag tggtcttgat
    26881 cgcggtcatg tgacgggacc ttctcttaac gaacgccttg acaaccttgc tcgcaagacc
    26941 gtcaatgggc ggattggtca gccagccgag attgcccggg cggtttattt tttggccgat
    27001 ggcacccaat cctcgttcat gacgggtcaa gcactggtgg tagatggcgg tgccactgcc
    27061 cgtttgagca cggagtgaaa acccttgcgc gcaaaaatca aacaacacat ccccgatccc
    27121 ctggtcgacc tataccgctc agcccgtgac aacctttcgc tggcattcgc gtatcctaac
    27181 gccaggtttc acccctggcg cgttgattcg atccaaaaac tggaagcctt gcgggatagt
    27241 cacggaggtg agcgctgcgt catcatcggc aatggaccga gtttgaagca gactaacctg
    27301 cagaaactac gcggcacctt ttctattggg atgaaccgtt tttatatggc tttcaacgac
    27361 ctgggtttca ccacctccat cctgctcacg gtcaataacc tggtcatcga acaatgcgct
    27421 gatgagctgc gcgggctgcc aatccccatt ttcgtatcat ggcgtgggcg gcgctatatc
    27481 caccccgcgc ctaacctgca ttacctgtac acctcttaca tcaagccttc tttcaacggc
    27541 gatgccaccg ggcggttgtg ggaaggcgca acggtgacct ttgtagccat gcagttggcc
    27601 tatttcatgg gcttcaagca ggtgattctc attggcgtag accacaattt caccagcaca
    27661 ggcaagccca atacaaccgt ggtatccaca ggtgctgatc ccaatcactt tcaccctggc
    27721 tatttcggta aagggtttcg ctggcagttg cctgaccttg agacatcaga aatcgcctac
    27781 cgcatggcaa aaagcgctta cgagaaaaat ggacgagaga tactcgatgc aaccatcggc
    27841 ggcaagctca ccgtatttcc caaggtcaac ttcgatacga tctggtaatg aaaagcgctc
    27901 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncctctttc gtttacgggg
    28021 atgttcaggt agtgaacgag aaggacgagt taatcaatat tctaaggtac ggcaactggg
    28081 gtttaaaaga gctgatgtct ttccacatta tcggtcaacc ggccgtgttt atacgccggg
    28141 cagccctgca aaaagcaggt taccttgatc tcagttatca attcctgtta gaccatcaac
    28201 tttggctgcg cctggcattg cacggcggta tgacccacgt tccacagttg tgggcagggg
    28261 cgcattacca cgccgggagc aagaatatgg cgcaagccgc cctctttggc aacgaagcgc
    28321 agcgcattgt gcaatggatg gaagcgacgc cggaattcgc cagtttatac gcgcaaaact
    28381 cgcgcaggat ttgtgccggc gcggaaaggt tgaacgcttt ttacctgttt gacgctcaag
    28441 attaccgcgc ctctttagac gcttacctgc gtagtttagc gctacatcca gccgctgccg
    28501 ctcaagattg gtaccgcatc ctcttcgccg ccctttctcc gctcggattg gatcaactaa
    28561 aaaaagccca cctggcgcgc cgtaagaaga ggttaaaatc cttccgtcca cctttaacga
    28621 ggaaataatg tcagactctg cccccacctc caccgccctc ttttttaagc cgtccaaagc
    28681 cgctgttctc attctcttgc tcatatttct gcttgccgga ctgggcgtcc gcctgatcga
    28741 cctggatgac cttccattgg attttgccgc cacccgtcag ttgcattcat tcatcatggc
    28801 tcgcggtttg ttctatgagc tggatactcc cattaccaga gcaattccag aagatatcag
    28861 acagttcgcc atcaccaccg gaagaagcga acccctgatc gaacctccgg tgatggaata
    28921 cctatcggca tatatctatg ctgtcatcgg taaagaaagt atgctggtac cgcgtttgct
    28981 gtcaatcgcc ttctggatcg tggggggcat tcctttattc ctgctgatgc gaaaaatcac
    29041 cactctcaac ggtgctttca tttccctggc gctgtacctt ttcaaccctt tcgccattgt
    29101 cgccagccgc agttttcagc cggatccttt aatgatcatg ctgatcctgt gggctttgta
    29161 cttccagttc aaatggatgg aagacgataa gctcgcaagc gcgatttttg ccggcatctt
    29221 caccgggttg gctgtactca ttaagacccc cgcggttttt ttcgtcggca ttccgatgat
    29281 cggtgtggtg ctgctaaaag ggctgaaaaa atcattacgc gattggcgtg tttacctggt
    29341 ggcagccatc tccctgctgc cggcggtgat ttataccgcg ctcagcgcca cagttggcgg
    29401 taacgccggc gccatcttcg gcgcgcgctg ggtgccggct ttattttctg atccaaaatg
    29461 gtacctgaac tggcttatgc tcgcccgatt ggtggtgggc ttcttcccca ttgtcatctc
    29521 gctgctcggg ttctttctga tccaggataa aaaataccag gcgttgtatt tttgcatgtg
    29581 gttgggttat ctactctacg gcttcatgtt cgcttaccac atctatacac ataattacta
    29641 ccacctccca ctcatccccg ttgtcgcgat gggttttggc gtgatcggtg gtgaactttt
    29701 ctctcgcctt gaaagcaaaa acctgagcag gttcttcagg ctactggtct tgctgctctt
    29761 cctcttcgca attggtttaa atgtgatgaa agcacgcggt gaaatgcttg gcaagagtta
    29821 caggcacgaa gccaaatatt ggaaagaact tggagaaaag atcgggcaaa acgcaaatac
    29881 catagcgctc acgcatgatt acggttaccg cattaattac tggggatttg ttcagccaac
    29941 cctctggcct actcagggcg atatgaccgt taaaaaactc attggctcca cagaccctga
    30001 atttgaggct ttgttcaaaa tgcgcactga aggcaaggat tattttctgg ttactctgtt
    30061 gggtgatttt gagtctcagg cagatctgca ccaatacctc tttgagaact acccctacga
    30121 acagggcgat gggtattatc tctttgactt aaaacatcca ttgggaaatt aggcttgatg
    30181 atgaacaggc agccattggt ttccatcgtg acgccttctt tcaaccagtc tccatatctg
    30241 gaggagacaa tccgctcggt tctcgggcag acctatgaac gcattgagta tatcctcatc
    30301 gatggcggat ccacagacgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    30421 ggctgaactg ctgcgccagc ggtgcttcgc ccagcatcac cggcgtgatt ggtgtgacgc
    30481 tctgcccggt atccagacca atgctcttta attgcttctt gaaatagcgc gcgttatccc
    30541 ataagcggtc caccatctcg gtggaatttt ctaaaagatc aaccgcagcg atacaggcgg
    30601 caacatccgc gggcggagtg gctgaagaaa acaagaaagg tctcccgcgt tggcgtaccc
    30661 actcgataat acgcgcgttg ccagccacaa tgccgcctac cacgccgaaa gcctttgaaa
    30721 gcgtgcccac ctccacatcc accttgccat gcaagccaaa gtgatcaacg atgccgcgcc
    30781 cacccctgcc gataacgcct tcgccatgcg cgtcgtcaac catcagcatg atgtcgtgcc
    30841 ggctggtcac ctcataaatt ttatccagcg gggccagatc accatccatg ctgaaaacac
    30901 catccgtgat caccaacgcg cggcgatatt ttccttcgtt ttcgttgata accctctcca
    30961 gatcggccac atctgtgtgg gcatatttcg cgataggcgc gcctgaaagc cggcagccat
    31021 cgatgatgct ggcgtgattc aactcatctg aaataatcag gtcgtctttg cccaccagca
    31081 ctgggataac cgctgtattg gcagcaaacc ctgattgcag caccacgacg gcttcaacac
    31141 ctttgaaacg cgccagtcgt tgctcaagtt cggtgtgcag ctgcatcgtg ccggcaatgg
    31201 agcgcaccgc gccaggtcct acgccaaatt tcttcaacgc ttcctgggcg gcttttacca
    31261 ttatcgggtg gtttgccaaa ccaagataat tattggagca aaaattcagc acttcttttc
    31321 catcaaccac cagccaggca ccctgcggcg aacccagagt tcggatgtga ttgtacagcc
    31381 cagccgcctt tagcgtgtca atttcctcct ggacccaatc cagtcttttg ctcattttca
    31441 cctctttttg cgtttgctat gcttcaccag tgattagatt atacatctcc ggcaatttcc
    31501 gccagtattc ccagttcgtt aagcagcctg aggatgccgt caacctgctt ttcgttaatg
    31561 accacgcaga gcgggtccag cgtttccagc gcgaacctgc cggtcgcctg cttgagaaac
    31621 gcagccctcg catcctctcg cgtgaagcgt agcaacagca ccctctcaat gaccgcctcc
    31681 tgcccatgct gactccagcg ctcctccagc acgcgcagat tttctggtat ggcagccgct
    31741 ttttctctct ccagcaactg gatcaattgg ccaaggcgta atccctgttc ttcagccttt
    31801 tttaaagtat ctgtttttaa tgcatacagg ctttctttgg ggaacacatg caccagctca
    31861 cagaacctgg ctacctggta gcgcggcagg cgcggagcga agtatggaat gctaaagagc
    31921 agccggtccc gaatcttcat cacacggttt tcttcatcac tgctgctcag ttccgcgccg
    31981 tcaagcagcg gtttcatctt ttcattgagt tgaaaagcgg tgatttttcc gttttccccg
    32041 gctctggcca ggttaactat tcccagccag tgcagcgggg cgccaagcaa ataggttaac
    32101 agcgcgcctt ccaccttttc ccaattctca aagccgctta gataagtgga agatttcgtc
    32161 tcgcgaatat accaggactc ataatctccc ccctggcgtt ggaaatcagg gttggtttct
    32221 ttaatggtcg ccagcagcga agataggctc caccaggtgc cgctctccag gccagcgaga
    32281 atctcagtca ataacctgcg tggggggatt ggatcgttct tccacgtccc ctcgaactcc
    32341 aaaccaggga gcaggcgaag ctcattgatt tcatttgatt taagccatac ctggtagaga
    32401 tgggtgagca cctggccgcg tgtggcattg aagaattctt tcaacgcttc aggaacgggt
    32461 tgcagtgtat catcaaccag ccctacctcc cgcagccatg gatagagaaa atcccggggt
    32521 atatcccagg cgttccaaat ttctgccggg atttccctcc gcatgcgcag cgctgccaaa
    32581 atatctgtca cgcgatctgg caggagtgct tcagccctgg caacccattt cggtttttga
    32641 ttcactgaag gacggatgtg cagcttgggg gctggcttcc ctgtgttttt tgctacttcc
    32701 agcagttcat cagggatata gaaaaattcc ttgggttccg cttcggttct cagaaaggct
    32761 cttcccgcca ggccggcgta ccacagcgtt tcagcggcac tcacagggtg caggtctggt
    32821 tcttcgcgtt cgcggctggc gcgaccataa tcacggatct gcccataaat tctggagaac
    32881 tccgcccagt ggatttttcc acctttttgc accaggtgat tccaggcttt ggcagcgcca
    32941 tcatccaggc cttccagcac tttctcaaac aagctggtat ccagcatgtt ctccagaagg
    33001 tcctggcgag cgctttctac ctcccgctgg ctgatctgta catcccaacg cctggcaata
    33061 cgggttaaaa aatccaggtc ataattcgct aaggttgatc tcagtccagg catgcgaaga
    33121 tctatccctg gtgattatac ctaatttgac tttttcaacc cagttaacaa atctgaatgg
    33181 ttaattgacg ctattccccc ttctatgtat aattccttag ctcccttaag gttcgaactt
    33241 aggatggtgg atggcgttaa aaggtaatct gaaggatttc tcatttatac agttgttgaa
    33301 cctggtcaac ctggcgaata aatccggggc tttgtatgtt gaaagaccgg gagaatctgc
    33361 ccgggtgctt ttccacaacg gcaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33481 nnnnctttgt atgttgaaag accgggagaa tctgcccggg tgcttttcca caacggcaag
    33541 ctggagtact tcgaatccag taatgagaaa cctgaattat tgcgttccct caaggatcaa
    33601 aaactgatca ccgcttacca acacaccctg ttgaaagaaa ggcttgaagg tttctctgac
    33661 cgtgaggcag gcatctacct ggttaacgca gggtacgcca ctctggacca ggtaacgacg
    33721 gcgttggaag aagattacgc caagaagttc cgcgctcttt tcgattggaa tgagggtctc
    33781 ttcaggttcg aagccggaga attactgccc gaaaacaaaa ttcctgtaca cctcaacctt
    33841 gagaacctca ttgtggaagg agcacgggag ttacaggaaa tcgaagaatt aaaggctgag
    33901 attccgagcc tggatatggc aatcaaattc actgatcgcc ccggaactga cattcggaat
    33961 atttgctttt cgccaactga atggcaggtg atttcttacg tcaatcctcg caataccatc
    34021 cagcagatcg caaccgccac taaattagac gaaattgaaa ttcgccgggt tgtttttacc
    34081 cttcttcaag ccggtctggt ggagatcatt cgccctggtg gcgtacccct ggcaattccg
    34141 ggaaagaatc ttcccgtcag aaacccggaa gaaaaccgtt ctttggtcaa acgcctgatc
    34201 gatcgtattc gctctattta aaaacacagg aacttttacg agatgcaaac tcagaccgtc
    34261 aaaattgtcg tcacaggacc cttcaacgct ggaaaaaccg aatttatccg gtctgtgagt
    34321 gagatcgatg tggtttcaac agaaaaacga atcagcgcga cggccgaaaa aattaaagaa
    34381 tctaccaccg tagcgatgga tttcggcagg atcacggtag ataacgacct ggtgcttttc
    34441 cttttcggca ctcccggtca gaagcggttc gatttcatgt gggaaattct ttcagaagga
    34501 atgctgggtt tcatcgtgat ggtagacagc accagaccag agacatttcg tgaagcacgc
    34561 agtatactgg aaacattcaa ggcgtacgcc cccacccctt acctggtaac ggccaataag
    34621 caagacctgt acgacgcctg ggatactgag gatatgcgtc tcgccttaag gctcgatcct
    34681 tctgtcaaga ttctcccctg cgaggcaacg aaaaaaagct cggtgaaacg tgtattactc
    34741 gaactgcttt acggcatcct gaaagaaatg gatgataatg aagccagaca gaacaaccag
    34801 gaagacgaaa tttcttgaaa tgagtcaaac gatgctggaa tataagaacg tgggattggt
    34861 agaggtgctc ggtgcagaag cgattgctga tttcgccaag gcgatggagg cacaaccctg
    34921 tgacccgatt gtacacggaa tactggtaag agcaggccgg gcgggttttc attactggtt
    34981 gacgcaagag ggagatacgt tggaaaaagc cgatgccagt tttcggcttg cgccgatcaa
    35041 gagaaaaatt aatcttggct tgcagcattt ttgcgccatt ctttcaaccg atacaaaata
    35101 tgcgcttgaa ttcaaagact ctgcaaacaa atgggagctt gccctgacag ccgttgagcc
    35161 agcaggtttg ttttctctgg agagcagtta cctttccggc tttttacagg agtttgccag
    35221 ctgggcgggg atgggcaggc tttaccaggt gcataaaaaa gttggcgatt cccctcatgc
    35281 tcaacacagc atcatcattg aaaaagagcc tgttgagtaa cgatcggcaa gaacggtttt
    35341 tcattttgat ttatcaaaaa ggctcacgaa acccccatga cagaagatgt atttatatgc
    35401 gtagatgtag aaacagccgg tccagttccc gggaagtatg ctatgctctc gatcggggct
    35461 tgcctggtct ttgatacaac cgcgacgttt tatatagagc tcaaaccaga taaacccgaa
    35521 gtcgacccgg aggcgctcgc catcagcggg ttggatatgg acgcgcttca gcaaaacggc
    35581 gtagaaccag cggaagccat gcgcgctttc gcggattggg tcaaggtcgt ttctcctgct
    35641 cctgccaaac cgctctttgt ggcttttaac gcgccattcg actggatgtt cgtgaacgat
    35701 tacttccatc gctacctagg tcgcaaccct ttcggtcaca gcgcgctgga tatcaaagcc
    35761 tatttcatgg ggcaaatggg ggtcaactgg gcagatacca caatgcggcg aatctcctcg
    35821 ttctttcttg aagaacaccg cctttctcac aacgcgctta gagacgccca ggaccaggct
    35881 gagatttttc gcaggatact tgaggcaaac cccaggttca atctagcgga atgatggttt
    35941 agaaacgcca tctgttgtgt atactgttgg tgtagttaac catcttttgt gtcggatttg
    36001 gaggaaccat ggcaaaaacg aatgactccc ataaggcgga cctgaaccgc atcattgaat
    36061 cggcgaaaag aatgggtgtt gagatcaaag aagatgaagc cctgcagtgg ctgacaaaca
    36121 tggccgctta taaagaaagt gggcaaattt cccttgacac ccgcagcgga gtatttggtc
    36181 acaacatcac catgctggat ttcagcgaca aggaactgga atacttccgc cgcattggta
    36241 agattgtaga gttcgaagat attccaggca aggttgaaac cgcgctggcg ctttcgggct
    36301 catcggctca atccaagatt cagacctatc ctggtgattg tgattacttt gaacgcatca
    36361 acattatcgc ccccacgcgt gatgaagcct gcaacatcct ggcggagatc atgcggaaaa
    36421 aggcgcttga ttacctgcaa ggacccaatt accagttgat cgaggtcaag ttcggcgagt
    36481 acccacagga tattgtcctc ggtacccgcg aagtaaaagc cggttctccc atttcctggg
    36541 atcccgcgca gatccgggag ggccgaatta cgggcttcaa gaaagacggc acccccgtaa
    36601 cggtcgcctg ggatgatgtg aagaacaacc cgggctggtg caagttagat tgggtggtag
    36661 cagatcccat ccgcaaacag ctggccaacg ccagtaatat gctggatgtt acctgggagg
    36721 ctccaggtgg agacataacc ccgttggatg gctaccttga cccctatttc caggaagttt
    36781 atctcgaggc gtcttctgta cccatttttt caaaactgtc gcagcatgtt tcagcgaacg
    36841 cgctggaaga atatgtgaac gccttagagc gcgaggttta taaatacctt accaaagacg
    36901 tgaactatgg caaggtggcc aagcgcatgt acaatatttt ccgcctcaca ggaaagtacg
    36961 aagaagctgc ctttttacgc gagctttttg acgaaccttc cactgttctg tatcagatat
    37021 ggtcattaat ccgcaccatc gacgatgcat atatcccggg ctcatcaatc accaacgaac
    37081 aaatggtgga atccaccgac cgcctgatct tggaagtgat tcgcgcgctt gaaggagacg
    37141 aggaaaccga gattgtcaaa catctcctca acctgcgcac ggccctggca aaccctgacg
    37201 cccgtgatat gctcaccgca gaagccgaag ccgcccgggc aagagtaatc aacatcgtga
    37261 ataacttctt ctacgaaaaa ctggttgcct atccaacgat taaagagtac atggacgaag
    37321 tgcaaaccag aagtcattga aacggctttg cctatctcat ggtcacagag ggttttcacc
    37381 tgaaggatgg aaaccctctt tctcgcggca ggtggcatcc agtaattgcg gagatacttt
    37441 tctcaaaccc gggtaacgca tggatggtag aataagcgta tgacgatcgt tacgcagtcc
    37501 ggctcccaat tcttcacaaa gaggtttgct tgaacaatag tcatcagagt atttcagaag
    37561 gtgttgcagt agaactgtac ctggacgcgg cggcagagga gcaggtgctg aaattccgtg
    37621 agctcattta ccgtgaaggg gtacaaccag tacaggggct gatggatgac aaacctcata
    37681 tcagtctggc ggtatttccc atcacagacc ctgccgcggt gatcgatctg accagggaat
    37741 ttgccctgac agtcccccgt ttcagcttca ggctcggtgc ggtcggtaca ttccctactc
    37801 aagacaacgt cctcttcctc tcccccgtac ccagcgtacc tttgctgcag gtgcacgcag
    37861 ccttccttac ccgggtcaaa gatgccggta tcaattgttc gccgtattac ttcccaggtg
    37921 tttgggtgcc tcacctgacg cttgagtttg aaatatcgaa tgatgagctg tgccgttcga
    37981 tgaaagtatt taaggaatcg tttaccccta ttgatggaga attcacccaa ctgggtgttg
    38041 ttcaattcag acccattcaa taccttgacc agtttgatct acagaaagga aattaatgtc
    38101 agaaggtgta ccacccccat cagaagagca aattcgcgat gccgtcaacc gcaggaaaac
    38161 caaagccgag gaatacttgc aggacccaga taaaacacgc cgcctgcttg aagaggctgt
    38221 gcataaagcc aatgatcatg aaggaaaacc ggggcagaaa ctagatttct ggctgcagtt
    38281 aaaagcattt ttcaggttgt tgcgagccta tacaaatcgt gaatacacgg tcgttccttg
    38341 gggatcgatt gtgatggtca caatcgccat tctctatttt gtctcgccga ttgatcttat
    38401 tccagattgg ctgccattgg ccggctttgt ggatgacgct gctgtcattt tattcgtcat
    38461 ccgccagctc aaacaggata ttgacggttt tctcgcctgg gagaacgcgc aaaaaaaccc
    38521 cggcggacaa atcattgatc tttaatttca agcacgctcc attttgatcg gaaaattcga
    38581 tataatggaa tgcaaactaa tgtataggag gagtaaatga gtaaaaagtg ggtaacggtt
    38641 ctattagtgg tatttgtgtt gatgtttgct ctttcagcat gcggctccct caaagatgtt
    38701 tcaaaattgg gagataagtt catgtcagcc cttaagaccc aggacaacga gacctcttac
    38761 gccatgcttt cacccgatat ccagtatgaa gtgggttatg aggctggctg ggctgaatgg
    38821 acctccatcc gcgcttttaa tgattggaag ttcaccagta agtccgttga gaataacctg
    38881 gcaacgctgg aaggcaccgc gcacctggat ggtgaagagt acctggtgac cttggctttc
    38941 acccgtaatg gtgacgcttg ggagataacg acaatcatat ttgaacttcc gtagcccaaa
    39001 aagtggagac tgcccgtctc cacttttttt tacaggaacg ttctcatcac tctctgtcac
    39061 actttataaa acaggatgga ttgcgggtct ccatcctgtt tcgtcatcac cagccaaaaa
    39121 gagaaacggc ccagtcattc tgcagagctg tatgtgacca ctcgtcaact tgcgccgccc
    39181 tacccagcat gatacacttt tacctatgaa gatcaacctg ctcgccttct ctgctgatcg
    39241 gcagctgctg accagcctgg aagtcatcac tttcaatggg atcacgctta ccaccacccc
    39301 ggcgggtaca agcagcgaat tgcgcgccct tacccagcag cagcgctttg acgccgcctt
    39361 gatcgacgcg gccgacggtc atctgcctgt ggtgcaaaca attctcaacc tcaccgctca
    39421 gcagccgggg attaaaattt tgctgctggt tgctgatgag cagaacgaaa ccctgccgcc
    39481 tgaagatatc gcttatgata gattgctgtc tcgacccctc agccctgagc aactcatcag
    39541 ttcactgaaa gaattgttcc tgcaacctga ggaaacctgc gcggaaacag gcgcagcgat
    39601 aagggaaacc ccagcagcac aagcagagca ggaacccagc ctgattgaga tcatcgacgc
    39661 tgcagaaaat gaaaaccgat tgacgggcga acaaggcggt gcgctgacca gccacacggt
    39721 gaagagcgcc ggtaatgcgg atcccaattt gattgttaaa gagttgcgcc tgagctactg
    39781 ctgcgtgctg ctgccgtgga gtcctcatca atacctgacg ggcgaactct ccgatcacgc
    39841 cgccgacttc cttccccgcc tgcaccacag cctgggctgg cgtatgaccg gcatctccat
    39901 ccgcccgcaa tatctgcaat gggtcatttc gttgccggtt gaaacctgcc ccgtcacggc
    39961 catccagaag atccgccagc gaacttctgc aaattttttc aaaacgtttc ccgacctgcc
    40021 ctccagtggg gagaacaggg acttctgggc tccaggctac ctgatgttga gcggcgtgca
    40081 aaccgtacct tccgccatta ttcacgaatt cgttaaacgc gcgcggccag ctgacggctg
    40141 aacagcagaa aaggatcatt tatgcctcca atcctctatt taatcgatgg tcacgccctg
    40201 gcctaccggg cgtattacgc tatcaccgcc ggaatgagcg accgtatgca aaccaaaaca
    40261 ggcgaaccca ccgccggtat ttttggtttt gccaatgtgt tgatgcgcct gctggagcag
    40321 gaaaaacccg agtacctggc agttgccttt gataccggca aaactttccg caacgagctc
    40381 ttccctgatt acaaagccac gcgggcaaaa atgcctgatg acctgagacc tcaaattgaa
    40441 cgcatccgcc agatgattga tgcctttcac ctgccaaggc ttgaaaaaga aggggtggaa
    40501 gcagacgatg tactgggctc catcgccact caagccgttg agcagggtct gggtgtcaaa
    40561 atcatcaccg gtgaccgcga cctgctgcag ctggtgaacg accgcatcat cgtcaacctg
    40621 ccggggtcgc ggcttgccga tgcaaaaaat tattctgccg ctgatgtgaa ggagtacctg
    40681 ggagttgagc ctgaacaggt ggtggactat aaagcgctgg tgggcgacac ctctgataac
    40741 atccccggtg tgcccggtat tggccccaaa acggccgtta ccctgctgca gacctaccac
    40801 acgctcgatg ccctttatca gcatctggat gaaattcccg gcaaaacggg ggaaaaatta
    40861 cgcgtcggta aagaatccgc ctatttgagc caggagctcg ctcgcattaa aaaagatgtg
    40921 ggggtaaggc tggtgcttga agacgcccgt actgaccacc tggacatcgc atcggtggaa
    40981 aacctctttc gtgaactgga gttccgtacg ctcatcccgc gcctgcgatt gatcgcggct
    41041 ccgctgaaaa aacaagaaac cccgcagctt tcactttttg gcgaagagat gaaggagatt
    41101 attgtgccag agtcctctta tatgattgag gtgaaactga tcgacaccgc gcaaaaactg
    41161 gcctcgctca aacaagcact ggatgccagc tccctcatcg cctttgatac cgaaaccacc
    41221 gcgctggatc ccctgcaggc ggagcttgtg ggcctttctc tcgcggtaaa agagggcgaa
    41281 ggttattaca ttccgctcgg tcataagggc gaacaacctc aacttaaggt cagtgaagtg
    41341 atcgaagcgc tcacaccggc gctcacaaac cccagcatcg aaaaagccgg tcacaatatc
    41401 aagtacgacg cgctcgtcct cgccaggtac ggcttgcggg tatctcctct ctcttttgat
    41461 acgatgatcg cggaatggct caccaatcct gcgagccgcg accttgggct gaaagatttg
    41521 gcaggtacat tgctgggtat ccgcatgacg cgcatcgaag aattgattgg caggggtaaa
    41581 aaccagctca caatggatga ggtgcccatt gcagcggcag ccccctacgc agccgcagac
    41641 gccgaggtca ccttgcgttt aatccctttg ctcaaagaaa aaatgcaaaa aacgaacagc
    41701 acgggcatct ttaataccat tgagatgccc ctggtaccgg ttttgctcaa aatggaacag
    41761 gaagggatct cgctggacat tgatttcttc aaacgcttta gcgcagaaat cggcgaacgc
    41821 ctgcgcctga tcgaagacga agttcatcag gcagtcggtc acgctttcaa cctcaattct
    41881 tcccagcagc tttcaaaggt gctcttcgag gatctcaaac ttgagcctcc tgaccgaaat
    41941 aaaagaacct ccagcgggca tttctccacc tcggcggcgg tgctggatga actcaaaggg
    42001 cagcacgtgg tggtggatct gctgctggaa taccgagaac tctccaaact caaatccact
    42061 tacctggatg ccctgcccca gcaaataaac cctcgtgaca atcgtgtgca caccagcttc
    42121 aaccagaccg gttcggtcac cgggcgcctg gcgtcatctg accccaacct gcagaatatt
    42181 cccacgcgta ctgaaacagg caggcaggtg cggttgggct tcatcgccgc gcccgggcac
    42241 aggttactct cagtggacta ctcgcaaatt gaattgcgga tcgtggcgca catgtcaggc
    42301 gatgaagcca tgctcaaggc gttccgtgac gggcaggata ttcacgctac cactgccgct
    42361 gccatctatg acctgccgct tgagcaggtg accaaagaac aacgccggca tgccaaggcc
    42421 atcaactttg ggttaattta tgggatgagc gcctttgggc tcagccgctc cactgacctg
    42481 acccttggag aagcagaaaa cttcgtcagg gattattttg agcacttccc cggggtcaag
    42541 aaataccttg atcagattcg cgtgctggcc gcgcggcagg gctatgtgga gaccatgctg
    42601 ggcaggcggc gttactttcc taacctggcc aacccgctga accagatgat gcgcaaccgg
    42661 gaagagcgtg aagccatcaa cgcgcccatc cagggaaccg cggctgatat catgaaaatc
    42721 gcgatgatcc gcgtacagcg ggcgctggaa gaaagcgcgt cccgcgccag aatgatcctg
    42781 caggtgcatg atgaactggt actggaggtt ccggagaacg aaattgcgct caccagccaa
    42841 cttgtccgcg ggattatgga agcagcttac aaacttgata tccctcttct tacagaagcc
    42901 agaagtggtg taaactgggg aagtatgcag gtgattgcat ctgaatagca ggctcgggag
    42961 cagccgatga acgtgacaga tcaggctttg caggcaaaac tgaataacgg acacgcggct
    43021 ttatgggagc acgattggga taacgcggtt agagtctaca ctgaagtact gctcagtgcc
    43081 cctgaaaacc caatcgccaa agccagtctc ggtctggcga agtttcacca gaagaagtat
    43141 attgaggcgt tgcagttatt tcaacagctt gccgcacaat atcctgaaga ccccatgcca
    43201 atggaacgca ttgcccgtat ttacgagcgc gaggggctgc tggctgaagc gtctaccagt
    43261 tttttccgcg ctgctgaact acacttaaaa gccaaagatg ttgaaaagtc tatcgctgat
    43321 tttcaggccg tcattcgcat cgagccaaat aaccagatcg cgcgcgcccg cctggcgttg
    43381 atttatgaga gactgggcag aaagaacgag gccgcggctg agttccttga cctggcggca
    43441 gtgatacagc agcatggcaa tatcgacaaa gcccggctga tgcttgaata cgcgttaaag
    43501 ttaaaacccg acgccgttga agttcacaac gcgatggcaa gtcttaagaa gaacgagcct
    43561 ttgcgcctgc gcgatcgaag cggagaaccg actggcgctg cgcgtatggc acaggtgagg
    43621 gagatagaaa cagcagttca ggaacccgaa gagctgaccg gcaatgaccc gatgactgaa
    43681 gcgcgggtga tggcgttgaa agaaatggct ggttttctgt ttgaagacca ggaaaccaac
    43741 cgtccggccg tcaaaccacg ggatcttcaa aacttcgtgc cagccagcag cgcgagcgaa
    43801 aaggcagaag cagccagcca caagcgggtt ctctaccaca tcagtgaatg catcgactta
    43861 caaacaaaag gagaaaacct tgaagccgcg gctgaactgg agcgggcgat caacatcaat
    43921 ctggacctct ctgccgcgca ttacctgctg ggatatttgc tccgtgaatc tgaccctggt
    43981 aaatcatatc cgcacctgca aaaatcagtg atggacccgg cctacgcgct cgcttcaaac
    44041 ctgatcatgg gagagatcca ttttgaagcc gcgcagttaa aagaagcttc ctccaacttc
    44101 ctgcatgccc tgatgctggc agatgctgaa accgtgccac ccgaggaggc tggcgacctg
    44161 ttgcagctct acgacccaat ctttgaatcg ctggctttta tcacgcatga gagggacctg
    44221 aggaaccttt gcgcggtcat tcgtgaacaa ttgagccgac ctgattggcg cgattacctc
    44281 aaagccgccc gcgagcagct cccgcccgcc cctgagggca gcccgccgct gccgctggct
    44341 gaaatgttga tggactccac cagcagtcag gtggtggaat ccctggctga agtgcgccaa
    44401 ctggcgatgc aggggaagaa ccgttccgcg atggaagaag ccttcagagc cctttcttac
    44461 gcgccctctt acctgcccct ccacattcaa atcggcgaaa tactggtaaa cgaagggcgt
    44521 attcaggagg cggttgaaaa gttcattcag gtggcgcgtc tctacaccgt ccgtggagac
    44581 gctgcgcagg ctatccgcct gcttacccgg gtgaccagac tggtacccat ggacatttca
    44641 gttcggcgca acctgatcga cctgctgcgc tccaatgggc gcaacgctga attgatcgag
    44701 cagtatatgg acctggcgaa tgtgcaatat cttctggctg accttgaaca atcgcgcaac
    44761 gcctaccatt ccgccctcat gcttgcccgc cagtcgcggt ctacccggga gcaatcgata
    44821 aagatcttga accgcctggc ggatatcgaa atgcagagcc tgaattataa agaagcggtg
    44881 aaagcctttg aacagctgcg cagcctctcg ccgctggata gatccacacg cgtcgccctc
    44941 gttgacctgt atcaacgcct ggagatggaa tccgcggcgc tcaacgaagt ggacgcctac
    45001 ataaagttgc tggaagacga gaatcgcctg ggcgatgccg aaaacttcct ggatgctata
    45061 ctgagtgaaa aaccagattc cgatggtctt caacgttgta tggtgtccta ttacacctca
    45121 cataaaaaaa ccagcactgc gatcgaaaaa ctggataagc tggcggagaa attactggtt
    45181 gagaataacg taaaagggtc gcttgcggtt gttgagaaga tcatctcgct caacccgccc
    45241 aatcaaacag aatatgaaag gctgcgcgcg gaacttctca agcgctaaac gaaagaccag
    45301 aggattggtc acccttcatc agatggatga agggttttta atatcctttt taccaccaga
    45361 ttctgaacca ggagcgcccg ctgccgtgct gccctctgaa agtgatatca gcgagcacct
    45421 tgacgaccga ttttacgggg atttgcgggg tggtgaacgt aaactggctg atgccgtgct
    45481 cattcgtttc gggcaggcgg tagaactcgc gtgtgtcagg cataaaactc agcgtcacgc
    45541 ccacctgcgc accctcaatc ggccgcgcgt attgatcttg aacgaccaca aatacgctct
    45601 gtgattcctc taccccgatc aatgattttt gggtaaacac ccgcacccgc ggggtcatca
    45661 agctgccact caggttggga ggagggttgg atttaagcaa ctcagggtca gccaccactc
    45721 gatcaaaata aagcttgccc aggtcagtca aaaccacacg ttcgcccgcg gcacggtctg
    45781 gccaccattc cattcgcgcg cgcgtaaaat actgcacgta acgcccatcc tccacctcca
    45841 cgtttgaaag cggcatgcca aagtgggttt cgccagcatg ggcttcgtaa tattgtaaaa
    45901 acgcgtaaca taccgtaaaa cctgtctgat acgccttaca ggcaggtcct tcgcggggga
    45961 tattggccag cgggttgcca gcttcgtgca gcagttcacc caaaggcgcg accctcacca
    46021 caggccctga aggggtttcc accaggtcaa agcgcgcctt ttcaaaatac tgcacgcgtg
    46081 atttgctaat ggggtcggtg aaatcatcag tgatagggta accaaaatac agcagcgggt
    46141 ctgacacact attgtaagtg cgcagaaatt ctccccagat ccagtgacct gtttcaggaa
    46201 cataaaccga atctgattcc tgcgcccgta ccgcccctga agacgacgcc cccagcagcg
    46261 cagacacgat cacgatcacc agcaagcgga taagttttcg catttattgc attatacaaa
    46321 aaccgatgac tattcgcaat cagaacgggt ttttccgcgc gccaaccgct gcccccgcgc
    46381 ttgcacatct tctgcggagg gatgcataaa accatgcggg ttagtttttt tcagcctggc
    46441 gatgaacgaa aacgccagaa agccggcaaa acccaccagc ccggcggcat accacatcgc
    46501 ccgcgagtag aacacgtcgc tgacataccc tgccagcagc agccccagac cggtaccgat
    46561 accccaggtg agcgcgtaca ggctcatata acgcgcgcgc atatcttccg gcgccaggct
    46621 ggcggtgaag gtggtggtgg tgggcacaag gatcatctcc ccaatagtgg cagccaccat
    46681 gcacaaccag aaccaccaaa aaccactgcc aaaggcaaca caaaagatgg caatggcgta
    46741 aatcagagcg cctgtcgtca tggctgtttc agcacggaaa cgattgacct gccgcgttac
    46801 caccaactga aaaagcacga ccatcaaggc gttggtcatc ggaatgaacc cgaacatttt
    46861 ttcagagacc ccgaaatttt gcttggcgta caccgccaac agcagccaca ggatcgaggt
    46921 actgatacga ttcaaggtgt atgcccccat cattaccata aacaccctgt ctttaaagat
    46981 cctcaggtac cccccgctgg ttggtgctgt caccaccacg ggtttgcctg acgctggctt
    47041 tgtctcaatg atgagcagcg tgacaaaaac cgcgatcagc ccaaacccca ccccaatcac
    47101 gccgaacgtt atcccatagg agatggacgc caggtagccg ccgatcgcgg gcccaaggac
    47161 aacgccgaaa ttattgccca tgcgcagcac ggaataagcg tccagccggt tggcttcagg
    47221 gatgagatcg gcgaccatgg cgtcagaagc caggcggtac aaaggggtgc tggcgcccgt
    47281 gaccaccagc agcagcgcga aaaccggcag tgtgccagcc gcacggaaga gaaaccagcc
    47341 cagcgcggaa aaaatcagcg agaagaccat caccccttta cgcccgaacc tgtctgcaat
    47401 ggcgccgccc atgaaggtgg tcaccatgcc gaccgcggcg ttgagagtca tcaaccaggc
    47461 cacgctggac agcggctcac ccagtcgttg gctgatgtac agcatcaagt acggccatat
    47521 caggctgtga aacagccacg ctagcagcat tatcaaagac accagccaaa actggcgcgg
    47581 gtatttcttc aagatagtgc taaacctgga aatcacgcgc ataattctaa cacgcacttc
    47641 tccaatccag gggagccgtc cattgacaga gcccttcttt tccagtaaat tgaatccgtt
    47701 taaacgatct cgttcaagga gcaaaaaatg ctggaagatt ttaagaaatt tataatgcgc
    47761 ggcaacgtga tggacctggc aattggcgtg atcattggcg gggcatttag caagattatc
    47821 tcttcgctgg tagacgacat cctcatgccg ccgattggtt tactgctggg taaggtggat
    47881 ttttccaacc tgttcgccag tttaaccagc gccaggtacg ccaccctggc ggaggcgcag
    47941 gccgccggcg caccgacact gaactatggt gtcttcatta acaatgttgt tgatttcttg
    48001 ctgatctccc tggctatttt cttgctgata cgcgcaatca accggttgac gaaaaaacag
    48061 gcggaagccg cccccgtgac acgcgcctgt ccgcgctgcc tcagccagat cccccttaaa
    48121 gccagtcgct gccccgcctg tacctccgaa gtgacgccgc aataaggcag gcgctgacga
    48181 aggttatgta aactccgcgc gccggtcatt cacctgctgc ccagaaaggg cgtctcactg
    48241 cagcgcgctg cttcactttc tggcaggttt gcgcccggtt gcaatcaaag cattctgtca
    48301 ggagcgtgct ttgcgggcgg gggttttaat caccccgaca cgataggaag gcgcgagtat
    48361 ttcaatgatc gagcacagcc gcgtatccag catgcggctg cggatgcgcg cgtctaaatc
    48421 ctccagcgtg gtggacatgg tgatgaccgt gggatatttg gcgttgtacc tttcattaaa
    48481 tatctggtac agtttttctc gcgcccaggg ggtggcgctc tgcgtgccca ggtcgtccaa
    48541 tattaaaatc ggcgcagaac gcacccggct gaacaggtca tcatacgagg tgccgcttgt
    48601 tggattaaaa gtagcccgca ggtggtcaag caggtcgggc accaccacaa acacaggggc
    48661 gtcgcccatg cctttgcggt aattgccaat cgccgccgcc aggtgtgttt tgccgcagcc
    48721 gtaggtgccg gtgagcacca gccaaccgcg cggattttct gcgaactcct gcgccgccct
    48781 gaatactttc tccaggttca actgcacttc aggggcaagt tgctcgggct cgcgcaggct
    48841 gaagttgccg aacgagcggt cggaaagcaa ttgcagttga tagagcagcg ggtctcgtct
    48901 ttccttgacg ggcgagcggt aatctggcgc gttgatatgc acggtcgtca ccaggtcagg
    48961 gtcctgcaag cgcgagcgga tgcggtcatc caggtcttcc aggtcaaggt tggtggtgat
    49021 gaccgtgggc agcttgcgcg tgtagcggta atcgataatt tgaaataatt tctctgccgc
    49081 ccactgggtg gcgttgtgcg cgcccaggtc atccagcacc aataaaggca cgttgcggat
    49141 ctcctcaaag cgctgttcaa aggtggcgtc ggtggcatca tacgagtacc gcagccagtc
    49201 cagcagatcc ggtacggtga ggaagatggt ggtcatgcca aattccacgc aggtgttggc
    49261 cacggctgcc gccaggtgcg ttttaccgca gccgtaagac cccatcaaca gcagccagcc
    49321 gcgcggtgcg gcggcgaact gctgcgcctg gctgagcgcg tattgcagcg agcggatttg
    49381 ctcatcagcc aagcccagcc tgccctgaac ggaataagag gagaaagaca tgttcttgaa
    49441 agccgccagg ttgctcaact cgtacaacct gttgcgggca gcctcgatca ttttattctg
    49501 ctggcacacg catggcacca gcttgccgaa ttccgggtgt tccagcggca ggtcgcgatg
    49561 taagaagccc acgccaccgc aaattgggca aacctctgag ggggcggagg gttcatcagg
    49621 tgatgatgaa gtcggcatac ttcccttcgc tatcccgttt gcgcttttct tcagcggatt
    49681 ttcgatcggt tccatcgcgc cctttctctt tccatgatct taatatcgct tcaatatact
    49741 gccagttacg cacattcctg gtgaccgcaa tgcggatcgc gtcttcgatc cactccgcgg
    49801 aataagtggc ttcggcatct tgcagcgttt cggagagtat cggcgtcagc ggtccgatat
    49861 tctgctcgta caggctgaat atatttgggc gttccctttg cagccgcaat ggcagtgact
    49921 gcgctgtttc agggtcccag gtgccgtttt tcagccctac cagggcggct tttccttgct
    49981 cgccgttgag aaagaaaaag cgtttatctt taaagcgtgc ttccagcagt gtgccgcggt
    50041 cgcaggcgcg ccgcagcgct tcttccagtc gggttgtttg ttcatccgtt gtcgtaccga
    50101 aagaagccat caaaaggtta tcttgcaaaa tatccatcaa acccaggtag cgtggttcct
    50161 ttatctgtcc ggatagatac cagaaggcgt agagagtaat gcgcatctcg tccaggttat
    50221 cgatcaccgt cagcaggtgg gtgaaaaagg tggcgggcaa cgggatcgat tcgttcctgc
    50281 cagcagtgaa tccgtcaaat tcaggcatga tggttttttt accttttttc cggcgctggt
    50341 tcactctcag caggggcggc gtttacaaat ttcgccagct tttccaggaa aaccactcct
    50401 atcccggggc tggttggacc gttcctgtgc tttgcaacga taagattcgt ggttttgtcg
    50461 ctttcttttg atttgtgaat gaaaataaca atatccgaat cctgttccag gctgcccgac
    50521 tcgcgcaggt cagagagcac aggcttcttc tcttcgcggc tttcaaccgc gcgcgaaagc
    50581 tgcgcagccg ccaataccgg cacctgcaat tcgcgcgcca gcgccttgag gctgcgcgaa
    50641 atatacgaga cctcctgcac ccggttttcg ttacgcgagt cgctgctcat caactgcagg
    50701 taatccacca agaccaggtc cagtttgtgt tcgttatcca gtcggcggca tttggtgcgc
    50761 atggccagcg gggtaatggc gggtgtatca tccagccaga tgttggtagc cgacagcaca
    50821 tcgatcgcct tggtgaacac atcccactca tggtcttgca gtttgccggt gcgcaggcgc
    50881 agggtatcaa tgccggtttc ctgggcgatc aagcgctgta ccagctgctg gttggacatt
    50941 tccagcgaaa acatggccac gttcttttta tacttctggg cagccgtttt ggcgaccgag
    51001 agcagaaagc ctgttttacc agtgcccggg cgacccgcga cgatcagcaa atctgatttc
    51061 tgcatccctc ccaacaactt gtccaggtca tacagaccgg tcggcacgcc ggatagctct
    51121 tcatcccttt tggattgctc gtctacatag tcatagtact cgctcaccac ctcattcacg
    51181 ggtttgaggt catggcgcac gcgtgacgcg ccaaggctga acacagactt ctcgacctca
    51241 tccaggatgg tgcggatgtc cttctcctgg tcgtacgcca acctggccat ttcattggcg
    51301 gcggtcagca tgcggcgccg aatggcggct tgctcaacaa actgcgcgta cgccgcgcca
    51361 tgcagcgacg agggcgtctg gttcaccaag cccagcaggt aagcctgtcc tccaatttca
    51421 cccaactgac cctgttcctg caggtcttcg ctcacagtca ggatgtcgat gggaacgcgc
    51481 ctttcgtgaa gccggatata cgacctccaa atccactggt tgcggatgat ataaaagtcg
    51541 tcaggcttga tgacttcgga gatctcataa taagattccg ggttgatcaa cacggatccc
    51601 agcacagcct cttcggcttc acggctgtac ggctgtgact ggataacggt ggcggcatca
    51661 tcgaaatcgc tcatggtttt ccttcttcaa cttttttaag agcgtggctt gtcatcttgg
    51721 ttcccctggt tgccctcacc cgccgcgctg tcatcctcct gcgtaccgct gtcaccagcc
    51781 acatcgtcaa ggttcacatt ttgcagaaat tcttcaaaca aagaaaggcg tttgctcgtt
    51841 tcttcgcttt ccatatcgct ttctgcttct ggtgtgggtt cgtcctcaac atccagcact
    51901 tcagcctgaa tatccacctc gggggtgaga ccagcttcac gcaagatctc gctgttcacc
    51961 aggatgggta cgtgcgccct caccgccagc gcgatcgagt cagaaggtcg ggcatccaca
    52021 tcgtacagtt ttccatccag ttcaagcacc aggttgccgt aaaacacatc cttcttcagg
    52081 gaaacaactt ccaccctgat caagcgcgcg ccaagtttgg tgagcgactg cagcaacagg
    52141 tcgtgggttt gggggcgggc gacttcgatc tcctgtaatg agatagtgat ggcctcggct
    52201 tcgtaaggac cgatccatat cggcagataa cgctcgcggc ccgattcgcg cagcatcact
    52261 atgcgttgct ggttggtcag gctcaccctc acgctatcga tgactacctc caccatacgg
    52321 gtcacaaaca acctccaaag gtactgctgg tttcgctgat taatattcta acacgtcggt
    52381 aaagcgcgcg caacttgaag ggagatgaag gacggtttct taaggaaagc ctttcaaaga
    52441 acttgctttt ttaaggatgg ggtataatac tcttactttg ccaggcgggt gcagaaatga
    52501 gttcagcgct tgagggtaac ttgtagaaag gcagcccgcc gatctttcgc acccgacaag
    52561 taaatatcac ctgctgtatt gccattcatc atttgtcgac tattgaaaca caaggcttta
    52621 tcgaattgtc gaaaccacgc attctactga tcgaaggtaa acgcaccgac cacccttcct
    52681 ttgccgccgg gttgaccaaa aaaggctatc tggtggaaag cgttcccagc ggcgcggctg
    52741 cgctggcgca gctggacctg gcggtgccga acctgatcgt ggtggacgct tcatccatgc
    52801 gcaccaacgg aaaacgaatc gttcaggcgc tgcgccagaa acagcccggg ctggcgctca
    52861 tcctggtgat tgacagcaac agcgacccca acgaccacta cgacgccgat gtcatcctgg
    52921 tgctgccctt taccatgcaa aagctcatca accgcatccg ccacctgctg cccgggcaaa
    52981 acaactatga cctggtggct ggtcccattt cgctggatgt gacagagaag cgcgtcacca
    53041 ttggcgacag gcaggcgcgg cttacgccgc ggctgaccat tctgcttaaa attctcatgc
    53101 agcacgccgg cgaagtggtg gaacgcaaag cgctcttcag ccaggcgtgg gagaccgact
    53161 acaccgtcga cacgcgctct ctggatgtgc acatgagctg gctgcgccag gccatcgagg
    53221 agaacccgcg ccgtcccaaa ttaatcctta ccttacgcgg agtcgggtac aggctggcgg
    53281 tggagccgat aacgaaacct cgccaacgca agtaatcttc caaaaaatcc cagcttttta
    53341 agtaagcgtt cttgctccaa ataccgggta atacgtaaga acttaaaatc agtaactacc
    53401 gataatcacg atagttattg tggtattggt atcttatttc atcaaatcga ttgacaatcc
    53461 tggttgtgaa tagaagttcg agccagaaac atcaaaaatg accttaccac tcccggtggt
    53521 aattatccgg tagccaaaca attgggtttc ataggttgtg gttgtaccag accctgccac
    53581 atcaattgtt ccatatggca cataaatcgt tcctgagaaa gttgaaccgc tgatccctac
    53641 taaattcatt tcttgcgtag atttctgatt ggggttactg gggggttcgt ccatataaat
    53701 caacagcccg catacacctg gcggaacacc agtcggcgta ctaccgccac tacaatttgg
    53761 cgctgacatg agtaattgga cgttggaccc aacattaaaa tttccatctt taatatagag
    53821 tgtaactccc tgtgcggtca ctcgcgcgcc agcattcgca tcaaaagcgt tcaaacttgt
    53881 gctgttaata cagtaaagac caggcgaaaa aattaatggg gttttgcctg gcgcttggat
    53941 gaatgtatag ttgccgggag aaattgttcc ccctttggtg ctacttccac gattaagcag
    54001 tcctgaacaa tctggtggtg gaatattaat cgatacaggt tgggtcgctc tttgaggagc
    54061 tggggttgaa ttgctggaat aatcagtatc aatatatgaa atcgtccccc cgccatacac
    54121 cgtaactggt ccatttccat acaaacatga gtttgaaaaa acgctgcccc caatcacatt
    54181 aatgggtttt gacgcagggt cgaaccctcc ttgaacacca caatttggtg atagtgatac
    54241 aatccccata ccaccacccg tagatccact taaagagact cttgatgtcg cctccactgt
    54301 ggttcttaat ggctggttgg taataacctg tccaaatcgt tgttctgata ttgttgacac
    54361 aatcgctttg atcttgagat aagtcttacc agcttcagtt ccacattcaa catccacgcc
    54421 attatttgta gaaagatcaa agggtaataa atccacacca tcgtcttggg ctgaaaggcg
    54481 agccgcgttc accgcggctg tcgcggcttg tgaagtcaac gttgagccac aagtgaactg
    54541 acttaagttt ccggctttca agacctgaga ggctgcccca gctccagcta atacagctga
    54601 atcagcagta ctttgatctg cccgccggga attgtagacc aggctgccgt caattgcaag
    54661 cgctgtaata cccaagatgg ctatcattgc caacgcaata atcaccaaaa cctgtccttt
    54721 ttctgttttc ctattcatta ttcacctctt tcaatttctg ttttcccctc taagggcgaa
    54781 gtacaattcc attagctgtc gcgcgtaatg aaacggtttg cccggtaagc gctcctagaa
    54841 agggcatggt aatgggaaat ggctgcgtca ctgtaatctc aattttatct ccagccttag
    54901 ggggtgtccc tactccacac gcgcgattat tgattagtat tgaaactgca actgatccat
    54961 ccttgggcag gtccgaagaa ctattccgta cacgattatt aatattattg cactcggtgg
    55021 ggaaacctga tgcataaata atcccctcct ctgctgcatc ttgcagcgcg aattgcacaa
    55081 atatcatgcg cccaatatca aaaattccag acagcagtat caacaagatt aatatgccaa
    55141 tcgctaactc aaccaaactc tgaccttgct ccgaattgat aacgcgattt ttataacgaa
    55201 atattctcac gaccctccca ccttttttat taaaactagt ttttcaatca catctttcct
    55261 aagaagaaat ccaaatcatg tataacgttt tagacatcca gaccgatcac caaactgcgt
    55321 gcgctggttg attcgatttg aattgtatta aaatttaaaa gaggaccaac aaaaggtaca
    55381 aaggtagccc tgacggtaac aacaacccga tcgcccaacg ccagtttttt accgctggat
    55441 accaaggctt cacaagagat ggggggtgtg gatggtcctt tgtcgtaact gatttctacg
    55501 gcattcaccg cgctcaaatc cacaaaggca ctcacccgtt ttgctctatc gcgaattcct
    55561 gcgcaatccc ggtatcgttc aacaccactc gggttcgtac cgcttgcccc cgcatagcga
    55621 acggcatcac ggctggcatt gcgtacggcg cttaacataa aaaccgcccg accgacttcg
    55681 aacaagccat ataacagcat taaaaaaagg gggataatca gggcaaattc aacaagcccc
    55741 tgagctggtt tcgctattct tctatccttg ctacgtagcc atagaagcat aaaattcatt
    55801 cagatgatcg ccatttctga atcacaaata ttccttttaa atcatatccg caggattatt
    55861 atacaaccat gcataagaaa ggaatatttt attgcatatt tgtaaagtag ggcgttaacg
    55921 gaggagcagc cccaaaaggc acgccagcag cgcagcgctg agggtacagg ccaggtttac
    55981 cacatcatta ttcagccagc cccaccccct gacgcggcgg gttggtttcc cacaactgtg
    56041 cagcgggtgt ttttcggtct ctttttcgca cgccgggcac cagtaaatca cctgaagggt
    56101 cgcgcctaac aggctgtcca ccaggctgcc aaacacgcca gcgagcaaga tgaccagccc
    56161 ccacaccagt ttttcccgaa aacttgtgtt gaccacaggc caacacgccg ccgccatcaa
    56221 cgcaacgagc agcgcgccgc ctgtggcagc cagcgtgccc gccaggctca cggcgcctga
    56281 cgtgcccgga ggtacccgct tccagtcggt cagcctgcgc ggccaggagc gattgagcac
    56341 ccccagttca gtcgcccagg tatccgcatt ggctgccgcg aacgccgcag cgaacgccag
    56401 ccatggtaaa accgatccag gcatgaccag ggagagcagc gccgccagtc ccgcaacccc
    56461 gccgtttgcc cctacctgcc aggcgtcgcg gcgtccgccc ttgaccgcct gttcctccac
    56521 cttctgtttc ttcaccctga acaggtgaga cagcccgctg gaggtgatga agaaagtgag
    56581 cagcaccagc gcccagcaga acccgccaat gccgaacacg accacgccca gtaaaaaagc
    56641 cgccagcgcc ccgctgcggt ccagcgagcg cgcgcggtaa gccgcgtaca ccaccagcgc
    56701 agccagcagc acaccaaaaa tcaattgaat cggggataaa ggaaccccca tgctacaccc
    56761 gtaccatggt ggtcagcaac accgcgatca acagcagcag cggggagagg atgccagccg
    56821 cccagatgag gtaactgatg gctctggttt ctgcgcggcg gtacaaaaaa agaccggcta
    56881 taaagtccaa cagaacaaag aacagggcga tcaccggcag catcaacagg cggttggatg
    56941 gaaccggggg catcggcagg gtgttgaagt catagccgat ggataccacc tcgcgggacg
    57001 ggatgaggag accggttgcc aacagcagca acagcgtgag gacagcgctc agcaccatca
    57061 gcacttttgc cagcggatct ttgaagacct ggttcatgta cgcggctgga atggtggtgt
    57121 gcgctggcag gggcgaaaga cttcccattt caatcacccg ctgaaagcgg cgggagaaat
    57181 cctcagggtc agcgggagaa agaatcaatg tcttttccag cgccgcaacg atcaccaaat
    57241 gcctcgcgct gctggcgata aattccacct cccccagctc ttcgtggtga gatgtgccca
    57301 gtatggcgcc gggcagcgcg aaccttggca gagtcagcgg ttttaccagg tcagatgccg
    57361 ggcgcaccca taacacatcg gttaccggca ggtcttctga gcgcagcccc cagcgcacct
    57421 gtaacccatc gcgctcaagg ctgtaatccg cttttaacag ggcgtaaccg cggtaagccg
    57481 ccagcggcag cagcataccc agtaaaataa ctcccagcag gtaaaagacc agcctccagc
    57541 tcggcggttg tgaaaatgcc agcacgagga gaaacgctat gccccccagc agaagcagcg
    57601 ataggaacgc gtgcagcaaa ataccggctt tcttgggcgg gtaaaacgcc tggcgctggc
    57661 tcattccacc tccaacctta ttttgtaacg cactgcgcca atacccggca aaccaggtca
    57721 acctgttctt ctgtcatctt acctgagaaa ggcagcgcca gcccgcgcct tccaaggtct
    57781 tccgtcacgg ggtaatctcc caactggtag gcgaacttat ccaccatgta aggctgcagg
    57841 tggatgggga gaaagtaagg gcggaccggt atacccctga gctgcagttt ctcggcaagg
    57901 gcatcacggt caagcccggg ggccacgcgg atgacgtaga cgaaccagga tacgcgtgtg
    57961 gtctcaggcg cgattaaggg tatctgcacg ccatcgatgt tcgccaggcg gctggcatac
    58021 caatcggcca cctgctggcg tttgttcagc aattcttcaa tgcggcgcag ctgcgccagc
    58081 cccagcgcgg cgctcatctc atccatgcgg tagttgtaac ccaggtaggt atgctgcagc
    58141 caggtatcac cgggcgcgcg cccctggttg cgcagcgcgc gcatgtacct ggccgcgtcg
    58201 gcatcatccg taacgatgat accgccctcc ccggtggtca tttgtttgtt cgggtaaaaa
    58261 gcgaaaacac cgtaatctcc catcaatccc aagcgcctgg atttatactc cgcgcccagt
    58321 gattcacagg aatcctcgat gtgcttcaga tgaaattctt ccgccagcgg ctgaagcagg
    58381 tcataatccg cgggttgtcc aaacacatcc accggcagca gcgctttaag cctggcagtg
    58441 tcgcctgctc ctttgcgcgg caaccagcgc cgcgccgcgg caccgccctt tcgcaggtct
    58501 tccagcgcct gcgcagccag cagcgggtcc atgttgccgg tcttggggtc cacatccaca
    58561 taaacaggaa tgccgccttc aaagaggatg gcgttggtcg aagcaacgaa tgaaaaaggt
    58621 gtggtgagca ccaggtctcc gggctgaata cctgcggcgc gcacgcacag gtgcaacccg
    58681 gcggtgcccg aactgacgcc aatcgcctcc ttcgcaccga caaattcagc cagggcggct
    58741 tcaaaatcgt cgatgcgcgg acccatgctc agatggggcg tgcgcaacac ttccataacc
    58801 gcctcccgtt ccgcatcgct gaggtcgggt aaggacatgg gtacaacagg ttcaacattt
    58861 ttcatggtgt gaatgatcct ctcttcgcgg cgcgtttgtg cgggttcaaa gtggttcctc
    58921 cagcgcgttc aggagtcgtt gcacttcaac gacaaggttc tccattggca cgacaaccgt
    58981 ttcaccgccg cggcggggct ttatttccag tccaccgact ctgaacgaac gttcacttac
    59041 tgtgatccgc agcggcagcc cgatcaggtc cgcgtcgttg aatttcactc ccgctgattc
    59101 ctcgcggtcg tccaccagcg gctcgagccc cgcgtcgcgc agcgcgttct caacacgcgc
    59161 tgccaacgct tcgctgtcca tttcttttcc cttgagcacc accacgtgca ccgggaaagg
    59221 cgccaggctg accggccagg ccagcccgcg ctcgtcgtga tgttcctcgg caacacaggc
    59281 gatgagcctg cccaggccaa tgccgtacga acccatgatc accggctggc tctgtccatt
    59341 tttatctaaa taagtgcatc ccagcgcgtc tgaataacgc gcgcctaact tgaagatgtt
    59401 ccccacctcc accccgcgcg agagccgcag cgccgctccg cattcggggc aggcatcgcc
    59461 ctcgcgcgcg gcgctgatat ccgcgatcat actcgcctgg tagtcgcgcc catggttgac
    59521 gttgcgcagg tggtatccct cttcattggc tccggccacc aggttggctg atttcgccgc
    59581 cagctcatcc acaaaaaccg tcacgccctt cagcccaagt ggagaggcgt aacctggaac
    59641 cgcgccaatc gaacggattt cgtcttcagt cgccgggcgt aaatcgctgg ctttcaccag
    59701 gttggccact ttggtctcat ttacttccat gtcgccgcgc accacggtga agataaacgc
    59761 ccatttctgt tctccgccgt cctcttccac cagtcccatg tagaacacgg ccttggcggt
    59821 gcgcgcgggt gggatattca aaaacgccgt caaatcatct attgaggaaa cgcgcggcgt
    59881 ggcaaccttt tccaggggca gaaaggcttc tttggcgggc tccattttcc gcgtggtggc
    59941 tgcctggcgg ttcgccgagt aaccgcaagc atcgcaaaac agcaggctgt cttcaccacc
    60001 aggcgtgagg tacatgtact cgtgagcctc ctccccgccc atcatgccgc tatcagagcg
    60061 caccgccacc accggcaagc cgcagcgcct gaagatgcga aaatacgctt cgtaatgttg
    60121 cttgtagcgg gtatccagcc cctgccaatc cgcgtcgagg ctgtatgaat ccagcatgag
    60181 aaattcgcgc acgcggataa gcccggcgcg cggacgcggg tcatctcgcc acttggtttg
    60241 aaggtgataa atgatcgcgg ggagctggcg gtaagagtgg acaacccccc gtaccaggtc
    60301 ggatactgcc tcttcatggg tcatcgccag caccatttcg tgaccagagc ggtctgtaaa
    60361 gcgccccatc tcgctgccaa tttgataata gcgcctggat tctttccaga tatcagccgg
    60421 ctgcacgacg ggcattaaca gttcctgccc gccaatagcc tccatttcct cgcgcagtat
    60481 gcgcttgact ctttcgagca cccgatacgc cagaggcata taggtgaaaa tgccactccc
    60541 cagctgacgg ataaaaccgg ctctcaccag cagttgatgg ctggcgaact cggcgtctgc
    60601 ggggttttcc ctcagggtct ggctgaaaag gttggagagg cgcatacgtt gttccaggta
    60661 attaaaaagg ctcaaatgac cattaaccag gcagccacgg cgcggtacaa ggccgtggcg
    60721 gccacatggt agctcttgcg catgatccaa cccaatatca gcgcctgcgc cagcgtgatg
    60781 agcacctcag gccaggcagt ggggaaatgg ctgagccagg ccctgccggg aagctgccac
    60841 aggaggtaga gaaaggcagt cgccagccag ccccaggtgg tcccgaggta agacatcagg
    60901 cgcaactgga tgtacccacg aaagatgctc tcttccgcca ggctcagtat gagcagcacc
    60961 agcaaaaggc tgcccttttc ttcggtgaac cccttgagca aaggggtgaa cttgccacgc
    61021 agaaagatgg tcagcaccgc caggagcaac cccagcatca ggctggcgcg ggtgttttct
    61081 ttgctccacc cggtgctctt gagaggttga ccgcgcgcaa ggaggagaat gatagccggc
    61141 agcaacgcga tgacagctat gagcatacgt aacgcgacat caccgcccac gaaggctccc
    61201 gcggctgttt taaggaagcc aagcagatta ttatcataaa cttgaaaggc aaagaagaag
    61261 ataagcgcga aaagagtcag cgcaaaggcc gcttccctcc tggaaaagcg gaactcgatg
    61321 cgcctgattt ttcgcaaaag cgggctggtc ccggcgatca tcactaccgc gatcacgccc
    61381 agccactcgc tcaggtaacc cagccagaac ttcacgtcca ttgagttcaa tctcccgcca
    61441 cctgtctgcc cctggcaggt cttttcggag tatccattct cttctcatcc gctggctcgt
    61501 ccagatcctt caccgcttct gagggttcct ccaggtgcag atccactttc tcttccatgc
    61561 gcacctgacc gcgcagaaaa gcgccttctt cagttgcgaa agcgatggtc accacgtctc
    61621 cccataccct gccagtggag cggatctcga gcttctcagc ggtgacattg ccgcgcagcg
    61681 cgccagccac aattacgctg ttcgcgcgca ggttctggca ggtcacgcga ccggtctcgc
    61741 ccaccaccac cagaccctga atgcggatct cgccctcaaa agttccctca atgcggatac
    61801 cccccctgcc gccaaggttg ccagcccaat tgatgcccgg tccgagcact gaattaaccc
    61861 gttcgatcgg cgccgtttgt tcctgcgtag caggagttgg ttttttaaac atccagttcc
    61921 tccacaggcg gattcacctg ttataacagg ggaaagagtc gttcacggaa aactacattg
    61981 agcgctctca ttttaccatt ctcaagccgt aatcaagaaa gcgcatgccc tgttcaatcc
    62041 gccggccagg tcgcgccggc gcgcacgatg ccgttctccg gcacatctgc tttcaccacc
    62101 gcactgttgc ccacgcgcgc gcccgcgcca atggtgaccc ccaggttgat ggtggcgccc
    62161 atgccgataa gcgcgcggtc tcccagcgtc accgcccccg ccagcatagc ccccggggaa
    62221 acattgacgg ttttacccaa gcggcaatca tgcgagatga ccgcggctgt attaatgatg
    62281 ctgccaaacc caacctccac ctcgctgccc acataagcgt tgaaaaacac ctggcagcct
    62341 tcagcgatgc gggcgttggg ttcaacaaat gcgcgcggat gcactacggt aaggcactcg
    62401 aaaccagccg ccgccagttt ttcatacacc gccagccgcg gcacaatgct gccaatccca
    62461 cccaccgcgt tcaccgcctt ccccagcccc tggcgtcgca gcccaagaag cagttctgct
    62521 cctcccaata cctcaatgcc caggatgcgt gtacccgcgg cgatgccgtc atccagaatg
    62581 ccgtggatgc ggtactgccc caacaagcgg atgagatcga tgagcgattt cgcgtgccca
    62641 ccgccgccat aaatgatcaa cgcggacggg tccacatcct cgaccgggat gggccgcatt
    62701 tttctgacca tttcttcagt aaccagcacg ccccggggta aatcttccag gtcaacctgg
    62761 ttggttttcg ccagggcgag ggcaggttgg gtgatgcgca atccctgagg taactcctcc
    62821 tgtcgcggca gcctgtctgc ggcaggtttg gcggtttcat gagggagcgg ctgcccgatt
    62881 gaatcagcca ggtaacataa ccgttcacct gaacgcagtg tatctccagc cttgaagcgc
    62941 aggttcagga taaaaccatc ccgttccgcg gtcagctcgt atgttgattt ggtcgtttca
    63001 aagctggcca gcacctgacc cgcggtcacc ttgtcgccct cgcgcacccg cagttctgcc
    63061 agcagcgctt caggctcatt ggggttgatc aaaggcacca gtatttgcag gggttcactc
    63121 atttcgaccc accacctttc ttacagcctg aacaatatct tctacatcag gcaggatctc
    63181 gttttctaag gaaggcgcgg cggccactgg caggtcgcgc gcggcaacgc gcactgccgc
    63241 tcgcaatctt ccgccgacct cttccagtgc ccgcgccata acctctgccc cccaaccgcc
    63301 ggtcaggttt ccctcttcca cggtcaccag gcgtccggtg cgttttatcg aatccgccag
    63361 cgcggtgaaa tcaaaaggag ccaggcgggt aggaatgacc agttccacaa agatctcatc
    63421 ctcgtaagcc agtttcagca tcgcctggcg ggccagctct gccatgtacc cataagccgc
    63481 caggctcgcc cgcgccgcgg gcgcgccgct caccttcacc aggtaggtgg gggttttcat
    63541 taacgtgttc ttcacgggtt gttcaaccaa ccagtgcagt tcagggaggt cttgcggctg
    63601 gtagacaggc agcaggtact gcagcttgtt ttcgataaac agcacaggtt cctcggtatg
    63661 caagataact tcctccagca gttcacccgg ttgctgagta ccgcctttac cggtcaggtt
    63721 cagcggcgca atcagcgtta atcccggtat gccgatgaaa agtttttcca gggtttgtga
    63781 atgcgtcggc ccgtagccgc gccccccccc cataggggcg cggatgacca tcggtaaaca
    63841 gactgaatga tcatacatcc agttaaattt gctgatgtgg ttgataagtt ggtccgcggt
    63901 gagcgtgatg aaatctccga acatgatctc cagcaccggg cgcaacccgc gcagcgccat
    63961 acccgcgctc acgcccgcca gcccagcctc ggagatgggt gccgcaagca ctcgctcagg
    64021 gaaagcgctg gagcagccct tcgtcacttt aaacgccccg ccgtatgggt cgaggatatc
    64081 ttcacccagc aggtacacat gttcatcttt ttgcagggcg cgcagcaacc cggcgttcaa
    64141 cgaggaaagc acggtagtgt tcatccgccc tcctcgagcc gcgccggcgg gtcagccagc
    64201 gcccgcgtaa aagccccgtt cacctcttca tcggtttccc cctgaaaccg ctccagctca
    64261 tcttttgcca atcttgaccc gctgatgagc agcgggtcgc ggctcgagcg cagcgcctgt
    64321 atttctgctt ctgcgcgggt gtcatctcct ttggaatgcg ggccaaaacg agcggtgttc
    64381 agcaccaggg cgtatggtcg cccttcacgc ctgaccaacc tggtcatctg catcgcattc
    64441 aggtggattt ccatcacatc gcaactgtct aactcggtta ccggtatgtc gaaagcctgt
    64501 agccgcccgg cgatatttcc cgccatcgcc agcgtagtgg gcgtggtttg cgcgatgtgg
    64561 ttattttcca ccacgatcaa taacggcact ttccataaag aggccatatt gcacgcctcg
    64621 tagagaacgc cctctccgaa agtgccatca ccaatgaaca cggtcacgat tgccccgctt
    64681 ttcttttctt tttcggccaa cgccatgccg gctgcaatgg gcatgatgcc cccctgcacg
    64741 ccattggaat agagattgcg ccagtgaatg tgttgcgacc ctcccctgcc gccgcaaacg
    64801 cccgtctgac gtcccatcat ttccgcaaat aacgcccgtg ggtctccgcc ataggcgata
    64861 aagtgcccgt gacagcggtg atttgtgacg atgatatcct gcgggtccag cgcggcaatc
    64921 actcccgcag cgttggcttc ctgtccgatg gaggtgtgcg tggtgccagc gaacacaccg
    64981 cgcttaaatt cctcgagtac cgtttcctca aaacggcgaa tcctgtacat ggtgcggtat
    65041 agcgtgattt cgtccatcaa gtccccgctc ggcaggaaca ggtgaagcgc cagggcattg
    65101 aacgccaggg ttcaggcgct gattgaatgc ccacccgcgg cgaagtgcga atttgtgcct
    65161 gctctatgat atatccttct tcaatgaaaa gcggactgtt tgcatccgtc aggtcggtcc
    65221 cattcaagcc accgtcaacc cccagcgctt tggttagttt cgccgggcca ttgcaccagt
    65281 tggcgggttc aacccccgcc cttctcgcgg caatccattc caatccctct tccggcaaaa
    65341 tggcgcgaat taaaaccgcc gcaggatacc cttcttcacc gcacacggta ttcagacacc
    65401 agtgcatacc ataggtaaag tagacatacg ctctacccgg cagcccatac attacctcat
    65461 tgcgccgggt tttacctgag tgcgcgtggc aggcaaggtc atcctctccg cggtaggctt
    65521 ctgtttcgca gatgatcccc gagacgcgcc gcccgtccac ctggcggacc aggcgctttc
    65581 ccagcagcgc gcgcgccacg ccagtcactt cacccaggta aaaggattgc ggcaagcaat
    65641 tcatatcaag tattataccc aaacagcacc ttcccgcttt aatcaaacgc cacccgcccg
    65701 ggtggtgttt gaaaaatcac ttgtctgctt gcattgatca ttcgtattgt gttttatctg
    65761 tggcggtgtc atgcgccagc gtgcgtttta atccttccag cagctccacc tcaggctcgt
    65821 acgccagtaa ttcacgcgcc aggctaatat cagcgcgtaa acggcttatg ccgccctcgt
    65881 tgcgcgggtt atataccact tcagggttcc cgccggttac ttcaatcgcc attctggcca
    65941 ggtctcttac agaagtttca gtaccactgc caacattgat cgtgagcttg ttcacatcgg
    66001 gggcgttcgc cgcggccagc atggcgttta ccacgtcatc cacgtaaacg taatctctcg
    66061 tctggttgcc gtcaccatgg atcacaatgg tgccgttctc ccacgcctgg tgtaagaagc
    66121 cgggtatcac cggggtgtgc accggcggaa tgcgctgacc aggcccatat gcattgaaaa
    66181 cccgcaagca taccgtttca atcccccaca gggaaccgat cgtttttaca tagtattccg
    66241 ctgccagctt tgaaaccgcg tatggcacgc gcgggttgac aatgacggtc tcttttaccg
    66301 gctgcaccgg ttggttgcca taaacagtgc cggaagaaat gaacaccacg cgcctgacgc
    66361 cgacatcacg catggcttcc atcaaagtca ccgtaccgcc tacattaacg ttgttatatt
    66421 cacgtggata cagcaccgac tcctgcacgc tcaccttggc tgccaggtga tagacacaat
    66481 ccacttcttg caggagcgac cacaaagttg gtcggtcatt gacatcaccg cgtacaaact
    66541 gcaccgttgg tgaaagattt ttttgatcac ccgcggaaag gtcatccacc ccgatgacca
    66601 catcgcctgc agccgtgagg cggttggcaa gcgctgaacc caggaacccg gccgcgccgg
    66661 ttatgagata tcgcatccag cctttttcct tatttaagtg cctgcattat agcatagacg
    66721 gatttatgcc ccagtggatt tttttcgacg taaggctgtt ttctcttcct ccgtcagaaa
    66781 agcggtgaga agagcgtttt cctgtgcctg gcgaatcatc gccgggctta atcctgcctt
    66841 cggcgccgcc acttcatatt catagcgcag gttgatggcg ctgatgccgg ggtcatctga
    66901 gttgatagag gcgagaattt tcttttccat aaaaagccgc atgggatgcg ctgcataatc
    66961 cgagaccaca ctggtttgca cattgctggt gaggttgcat tcgacgccga tgcctttctc
    67021 acgcagaaaa tcgagcaacg cggggtcttc tggcgcgcgt accgcgtgac caatacgctc
    67081 cgccccgagc tcgcgcaccg cctgccagat cgattccggc ccggcggatt ctccggcgtg
    67141 cacagtgata tgccagccag cgtctcgtgc cttacggaaa tggtcgcgga acaattcgcc
    67201 gggaaagccc tcttcatcac cagccaggtc caacccggca aactcatgag catacctgag
    67261 ttccgcctcc aattctttca tggcaatctg cggtccgtat tgccgtgaaa ggattcccaa
    67321 ctggttgaca cgaatggggt gttcctgttc tgccgcgcgt ataccctccg tgacggcttc
    67381 caccacgccc tcaggctgca agccgtgggc ttctgccatg aaccaggggc tgtaacgcag
    67441 ttcgatgtaa tcaatccctt ctctgcaggc gtcttccacg ttctcatagg cgatgcgccg
    67501 gcaggagtcg taatccacca tcacgccggt catccatttg aacttttcaa taaacgccat
    67561 cacccctggt tgcggtgtgg taacctgtac aaacgggcgc agagtatcaa gcgttttgcc
    67621 aggtaatggc aggttgtgtt ttaaccccag ctcaaggatg gtttccaaac ggatgctgcc
    67681 atccaggtgg cggtgcagtt cgaccaaagg aatatcttgt gaaatcacgg ttcatcctcc
    67741 tgttgttcgt gaatagtgtg tagttttaag ccaagctcct gcgaatattg aagaatctct
    67801 ttcaattcct catctgccgg gtatgaaaag tagagggttc cctcctccac ctgtcctgag
    67861 aggtcaatgc cgccccagaa aacctgcgtt tcagcaggca ggcggctgag cagctttttc
    67921 agctcttcca ggctggcaac ggaaattttc agatagtcgc cctcccaaat attctcagcc
    67981 gccgtaatct cagcgaaaga cttggtgcga ttggtgccgg tggtaagcgt gtatacgcgt
    68041 gttttgccct gttgccaaga atacagttcg taccctttca gtgacgcggg taactgctct
    68101 gggatcgtgg accaatccgt tttggggaat accccagtct cttggttcag ccctgcttgt
    68161 tctccgccgt tgcaggctgt gagcacccac aagcacaggc tgaggattat cgccacttta
    68221 ctcgctttca tcgcttccct catcaagctg ccggaccatc gcgcacctcg atctctttcg
    68281 acatcagcac gatctccggt ctgcgtaccc aaccatctgc ctgccagaat tttaaaccgc
    68341 tatgattagt agtatagacc ataatgtggc acttatgaat atctctcttg tgtaaagccg
    68401 tgaagacggt gcgcaccaaa gcgctgccca gccccctccg acggtactga ggtagaaccg
    68461 ccaggtgata gaggtaacct cgcctgccat cgcagccaca caggcaggta cccaccagtt
    68521 gaccatcctc ttctgcgata tacgacaacc cggcgttttg ctggataaaa cgggcgagcg
    68581 cgcctggttc atctgcgctg ctcaacccca tgccttctat ttgcttccat aaggcaagcg
    68641 ccgcctgaat atggagaacg gacatttcat tgatctgtat ggtcatttga ttgctcgtct
    68701 aaaagaatag actggtcttg cgggaaagtt ttggcgtgct tcttttctgc gggcgggaac
    68761 ggaccccact gcacgcgtgc ttcctcgtac tgcgttcgcg gctcctttgt ttctgccggg
    68821 tagccaatgt aaatgaggtt caagggggtc acgcctttcg gcaggttgag gatatggctc
    68881 acctgtcggc taaaaatggt cacggggtgc acgcccaccc agatagaccc caacccaagc
    68941 gcggtggctg ccagcaggat gttttgggtg gcggcgctgc aatcttgcac ccaaaacttg
    69001 tcgcccactt tccctttttg catgcggctg ctgcccagca cgcagatggc tgcgggggcg
    69061 ttcattttcg cgaacattaa cgcccgacgg aatttatcca taactgactt ctctgtaatc
    69121 accacaaact cccagggctg cgcgttcatt gcgcttgggg cagccatagc cgcttccagc
    69181 agctggatga tcttttcctt ttccaccggc tggtcggtga attttcgaat gctgcgtctt
    69241 ttcatgatca cggtaagtgt atcttccaaa gtattactcc ttttttacct cgcggtttgg
    69301 ctcgccagcg gccaagtgaa catgcagctg caggatcgat tgggattcgc cgtcattaat
    69361 gatcaggcgg aaccgacttt tttcctatcc ttcagcccga agtgtttgct gggataagcg
    69421 cataaattcg ctgtaaaaca tcggggaatc cacgggaaag gataatccat cattgtccga
    69481 ttttcgggca cgaggagggt gtgcataggt agtcgggttt tgcgcgcctg accgccatcc
    69541 agtgttccat ccctccaagc cttccgccgg gcatcagggt gagggtgtgt gtataacccc
    69601 atccaatcct tttcatgaaa atgcagctat aagcaagttc tcgcatcatc tcactcatcg
    69661 ccctccagtt ccattgggac cagctcagag ccaatcttat tataagagtt ttttgttggc
    69721 agccgggaga tcattttgcc cccccctttc ttttacgggt agccaatcat tagcggtttt
    69781 tttagcgcgc tggattttgc gcaagggcag tcagttagga attgcgcaat atcaggatac
    69841 tcttaaaata atctgcagat gctagagcat aagctatcac agaccttttc ttctttgcag
    69901 caccccaact tccgtttatg gttcggtggt cagatcgttt cgctcatcgg tacatggatg
    69961 caagccaccg cccagggata cctggtatac gaactgaccg cgtctcccat gatgttgggt
    70021 attgtcggct tcgccaacgg cataccggtg tggttgttct cgcttttcgc cggcaccgtc
    70081 gctgaccata tttcacggcg aaaaatgctg ctcatcaccc agggttcaat gatggttctg
    70141 gctttcatcc tggcggtgct caccttcagc ggcaccgtgc gcgcctggca catcatcctc
    70201 ctcgcgctcc tgttggggac ggccaatgcc ttcgacgctc ccggtcgcca atcatttgtg
    70261 gtggacctgg tgcccaaaca ggaactcacc aacgccatcg ctctcaatag ctcggtgttt
    70321 aatctgggca ccatcatcgg tccggcggct gccggtttgg tttacgcctg gctgggtcct
    70381 ggatggtgct tcaccatcaa tgggctttca tttatcgcgg tgctgttggc gttggcgttg
    70441 atgaaaatcg ctgccaaacc ccctttacgc cctgcgcagg gatcactggc tcatacactc
    70501 accgaaggag tgcgctactc gtttaaagac gcgaacatcc gcgttctgct ggttatgctc
    70561 accgctagcg cggtctttgg ctttggattg ctggcactca tgcccgcctg ggcgacaggg
    70621 gtgctgggcg gagatgtgcg caccaatggt ctgctgcttt cagcgcgcgg tttgggttcg
    70681 ctgagcgcag cgctgatgat cgcctatctg ggcgcgcgca tcatccgtgg caggttatgg
    70741 agcatcggca gcctggtgat gcctgtttcg ctattgctgt tcgccctctt tcgtctgctg
    70801 ccggtctcct tgctgatgct ggtattgatg ggttggagcc tgatgaccgt ggtcaacctc
    70861 acgaacgcgc tcatccaaac ccacgtcccc gacgagctgc gcggcagggt gatgagtgtg
    70921 tacattctgc tgtttcaggg tggcttcccc atcggttcat tggtcgctgg ttggctggca
    70981 gggctgacca gcgaagccct cgccgttttt gtgttcgcca gcgtattact ggctgtgtcc
    71041 atcgccatcc aactgcgcca accctccata cggcagtaca actgatgcat tgatgaccgg
    71101 cgttcgtgca catcgcttat aatgttcact ggttggtttc atcctgtttt tcaagaaagt
    71161 taataaaacc tcgagatgcg cattgaacct gaaacttcca tcagccacga ccatacctcc
    71221 acggaaggag gtgttcagcc caggcggaaa agaatcaaga acagggggag gcttgtcgtc
    71281 aatcttttcg ccgcggtcgt tttcctgatc ctgctggcgg gttacctggc aggttggttg
    71341 ggagaatacc cggcgcgaaa cgctcagctg atcaccggca gaattgcctt ccttttcctg
    71401 ctggcttcgc tgagtatcac tcccctgcgc acgcttaccg ggaacatcca tattgggccg
    71461 ctgcgacagg cttttggctt gaacgccttc tatttcgcga tggttcacgt attaatcctg
    71521 attttcctga attaccgaat ggatttttat gctttggtca aaggtttttc caatcatcca
    71581 tacttttggc caggagcggt cgcttttcta atcctcagcg tattggctgt gacctcgctc
    71641 aacaagatga agagagccct ccgtggttca tggaagaaaa ttcacacgat ggtgtacccg
    71701 gcaggggtgc tggtgctggt gcattactca tggatagcca acgggaaatt cattcccgct
    71761 gcagaggtga aaaccttacc gctgctaacg gctgcctacc tggcggcgct gttcacctta
    71821 aggttaaggc cagtgaaaaa ggctgtgttg cgctggcgga aaaaagcaaa accccgcaca
    71881 ggttgagcgt ggctggtgtt gtttgacagt gtttcttttt aggatataat aacccccgaa
    71941 gaacggtcca ctagctcaat tggcagagca actgactctt aatcagtggg ttcggggttc
    72001 aagtccccgg tggatcacta aatgtggggg taattgagtt gaacaaccac caaaatatcg
    72061 gtttataccc gttggtatga cctttcgggt atgcgctgcg ttcgtattct ccggcttgca
    72121 ggtacttggt ctttccgcct gtgtgacact gttgaacagc acccgcacca tctcccgaac
    72181 agcctctaat ctgttgcgca atatctcttc aatcgtccct gcagacaggt tgcactcatt
    72241 ttgatgagtc atttcccagt ctctcttgga aaattgtggt gaataaccga ggctgtggat
    72301 taaattaatc actataaata taaattcttg ccgcgaataa tctgaatctt ataaggaaag
    72361 tagttggtgt aacatatttc ctgtaaattg ttgaacctta acaaaagcgg tgagtcagaa
    72421 gtatccttaa ggtgaccaaa ccttacaagg agaacaaaat gactcatcca aatgattatc
    72481 ccttcataga cgaaatagta agaaaaagat tgtatgcaat cccagagctg atgcgagtgc
    72541 tgatcaacaa tgccattcgg aaaatatact tgtgctggca ggtcattcgg ttgttaaaga
    72601 acgatgggta tttccgacgc gaatatccaa aaaaggtcgc gtaatcagtg aatggtcaaa
    72661 gagcgtttga gtgggtataa tcgttaatat tgaggaggat atatgacaga agaaaataat
    72721 tcgtcagtag atacttcact ggaggcgcca aagcccaaaa aagcgctcag ttttttctcg
    72781 attctgagtt ttatcgcggg tattggtaca tacgtatggt tttttgggat ggtctcttcc
    72841 aaacccatac tgacttttct cctgaccccg tttttcgcgc tcatcgctgt gatcactggg
    72901 cataaagggc gtcacgatat tcgcaagagt cttatggata tgaaggggaa aactctcgcc
    72961 aatatcggct tattgctcgg gtacgtgatc atcctgacgg ggatcttcat tatcgtgctg
    73021 gccgtaatgg gagtggtcac cattgccggc ctgctcggtt cctgatcatc caaagaacca
    73081 tcaccctgta acaattagaa ggagtgcctt accatgactc taccacccag acttgaaata
    73141 ccgccagcgg cggtaactcc gccatccata aacccaaaag acagcccgct cgcgcttacc
    73201 agtctgatca ctggcatcct cggatggaca atccttccgc tgctgggttc catcgcggcc
    73261 atcattaccg gtcaccttga aaaaaaagaa atcaaagaaa gccacggcgc gctgaccggc
    73321 gacgggatgg cgaccaccgg cttgatcctg ggctacattc aaatcggttt tattgttctg
    73381 agcctgatcg cggttatcat tctgctgctg gttcttccca agaacaactg ggggttgtct
    73441 ctctgatcca cctttcacag gtgaagagcg attcactttg catcgcacct gcaaccttga
    73501 acccgaagct tcgtatatct aacaggtgaa aagaattcaa caggagaata aaatgacaac
    73561 gcccccttcc aattatgaac aaggagttcc agtctttccg atagtcaaac gtgaaagcag
    73621 cctggccatc atcagcctgg tggccggcat catcgggtgg acgatcatcc cctttatcgg
    73681 ttcggtcatc gccgtgatca caggtcacct cgccaagaaa gagatccgtg agagcggaaa
    73741 cacgatgagt ggagacggca tggcccttgc tggcctgata ctgggctaca caatgatcgg
    73801 catctcgctg cttgccatcc tggtcgttgt gctcgccttt ttactaatcg ccaggtcaga
    73861 acttggcctg taaaaaaata acgcctgacc aaacacagcc atcgagccta ctttaacctc
    73921 ccggtgttga tcactcggga ggttgatttt tctcgggcga tcttccaccc agcgcgggaa
    73981 cagggaaaaa agcctggaaa gacgcccgtg aaaaaggatt atggtaaact tatcgcgcta
    74041 tctgaaaacc atcttgagga actgctttat gcccgcaatt tacccattta ctgcaatcgt
    74101 tggacaggaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    74161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naggaactgc
    74221 tttatgcccg caatttaccc atttactgca atcgttggac aggaaagaat gcgccgtgcg
    74281 cttgtgttaa acgctgtgga cccacgaatt ggcggagtac ttatccgtgg tgaaaggggt
    74341 acggcaaaat caaccgcagc ccgcgcgctg gccgcattgc tacccagcgt caaagtagtt
    74401 gcggagtgcc gttttggctg cgaccccgaa aaacccgcca cctggtgcac cgaatgccgc
    74461 gaacgcgctg aaagaagtga aaaactcgaa gccatcaccc gccacacgcc gtttgtcaac
    74521 ttgccggttt cagccactga agaccgggtg gttggcacac tggatattga aaaagccatc
    74581 caaaaaggtg tgcgcagttt tgaaccaggt gtgctcgccg cggccaaccg tgggctgctc
    74641 tacattgatg aagtgaacct gcttgacgac catgtggtgg atgtgctctt agacgcggcc
    74701 gccatgggtg tgaatatcgt ggaacgcgaa gggatttcct tcactcaccc ggcgcgattt
    74761 atcctggtgg gaaccatgaa ccctgaagaa ggtgatttgc gacctcaact gcttgatcgc
    74821 ttcgcgctat ctgtcgagat tcgcggcata cgagatgcgc gcgaacgggt tcagatcatg
    74881 gaacgaaaca tcgccttcga agaagatccc aaactctttt acgattcatg ggaagaaaaa
    74941 gagcaagagc tgtcgcacca gatcgagaat gcacgcaagt tggtcgatga agtaacctac
    75001 acccgccaga acctgctttc catcgcagcg ctaacctcat cgcttaatgt ggatggtcac
    75061 cgttccgacc tggtcatcct taaaacatca cgggcgcagg cggcattcga cggccgccgg
    75121 cagatcaacg accgcgacat cgctctcgcc gctgaactgg cgctccctca tcgcctgaag
    75181 agcgggccct tccaacgcga agagatgggc atggaagaac tgcaggaacg cattgagcaa
    75241 ctgcagggcg gtacttccgg cgagaatgaa gaagaaacaa tttccgaatt gggtggcgag
    75301 gagacaggca gcccaaaaaa agcctgacgc cggaagagac cggcggaccc tccccgcaac
    75361 aggggcaacc agaaccgagc ctgcagaacc cgtttaacaa gaacgatcct ttttggtggg
    75421 c
//
LOCUS       DAOSPM010000003        43014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_937_length_43014_cov_16.128122, whole genome shotgun sequence.
ACCESSION   DAOSPM010000003 DAOSPM010000000
VERSION     DAOSPM010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 43014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43014
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_937_length_43014_cov_16.128122"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1148)
                     /locus_tag="PLW25_00785"
     CDS             complement(207..1148)
                     /locus_tag="PLW25_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS53052.1"
                     /translation="MSGLETTITDFLKLTFLSMVPFVLASQGTMLGGRTGVFNVAQEG
                     IMLVGASVGFLVSYTTGSNLIGALVAMAAGGLFGLVLAYFTTTLKMDQFVVGLALFFI
                     GTGLSSLLPKLVIGITLTPPLVKTLHEIPIPLLSQIPIIGPVLFKQNILVYFSILLSI
                     FLWYILFRTNMGLRLRSVGENPKASDALGINVVSNRYITSIIGGMLIGLAGAYLPMVY
                     TGTFTDLMTKGRGWIAIALTFFGAWSPLPILIGSLFFAAIEVLSFRFQVGGSFIPYQF
                     LSMLPYIATIIVMIFTFGKSRVPAFLGQNYDRERRTL"
     gene            complement(1145..2158)
                     /locus_tag="PLW25_00790"
     CDS             complement(1145..2158)
                     /locus_tag="PLW25_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS53053.1"
                     /translation="MTQIKKFFPYLVSVLAAFLVVGVLLWALGFNPIKAYQTILFTSF
                     RTPNGFVQTLLKWIPLTLTALAFTVPLSSGKFNIGAEGQMLVGAIGATAVGILFKDLP
                     PALLLPLVILAGMLAGSFWGLIAAWLLYQFNINEILSTVLLNFISFALVDFVATGPWR
                     DPAAGHPTTIPIGANGFLPVLVKNPPLHSGIILAVIVAIAVYIFTSKTTSGYELLVTG
                     ANPRASKVHGIDTRKMFLFSLVIGAAVAGLAGVIEAAGTHHRLIEGLHSNFPTLGLII
                     GLMARGKNQAVPFVAFFISILEVGGSAMQRTIMVPVQIVLIAEALILIFILLSDIIKR
                     RQK"
     gene            complement(2177..3724)
                     /locus_tag="PLW25_00795"
     CDS             complement(2177..3724)
                     /locus_tag="PLW25_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53054.1"
                     /translation="MEQENVLQMQQVNKSFDRVVALCNVDFELKPKEILGLLGGNGAG
                     KTTLMNILYGLYRADSGTILLKGQPVTIHSPRDAIRQGIGMVHQHFLQINNYTVLENI
                     ILGSKVSHPLSANYQVEEAAIKELCKRFGLEIDLSARVETLPMGTRQKVEILKALYRG
                     VNILILDEPTTNLIPQEVDSLFETLKIMVREGLSIVFITHKLREVLSICDRITVLREG
                     KNVVSLTRKEASDEALVRAMVGDKMDLEKSVMFTSHEDSATVAGSQEVLSLEGVSLIS
                     QHKIQQLTDISFSIGSGEIFGVAGVAGNGQRELAEVVMGVQPVTSGKINFNGQEITTH
                     STKERIAEGFVYVPEDRLSDGFLNKASVAHNLILGYHNLEPYSKKGIINWKNVHENSR
                     KMISEYNIKTTGPDEIGGNLSGGNIQRVMIARAFSQPSRLMVAHNPTRGLDIPSMDFV
                     YQKLLGNSATGTAILLLSEDLDELLLICNRIAVMYRGKVVGILPREKFEKYEIGRLMS
                     GYEQKSK"
     gene            complement(3829..4914)
                     /locus_tag="PLW25_00800"
     CDS             complement(3829..4914)
                     /locus_tag="PLW25_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HOS53055.1"
                     /translation="MKKNLLFAISVLLVLSVLLSACAKPTAAPVATEPPAVQETEAPA
                     ATEAPAAQEPAMEVKLAAIFPGVITDNDYNTLAYVGMTAAQEQLGIQAVYSESVPVPD
                     VDRVMREYIDAGFNVIWTHGGQFVNQTAELAKLFPEVVFIAEGDAPIADPSPNLWFID
                     RNFNVGFYGIGAAAALASQTGKIAYLGGQALPFSYQEVHAIQQAISDLDIKVELVPVW
                     TADFNDPAKTRQLADALIADGVDVIMGSVNLGMVGVFEAAKATTDKKILITAKYSDKT
                     SFGPDNYITAVLYDFSKPLVDIITSIEGGKTGGYYPLGFDTGVALQLPLQNVTPEVAA
                     KVEEVINKVISGEIVVVKDSTEVTSAK"
     gene            5351..5551
                     /gene="rpsU"
                     /locus_tag="PLW25_00805"
     CDS             5351..5551
                     /gene="rpsU"
                     /locus_tag="PLW25_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOS53056.1"
                     /translation="MASVVLRPNESQDSLLKRFRKKVVKSGVLSTIRRKRWFVSKSEL
                     RRVEKKKAIRRIKRRQYTRVGE"
     gene            5598..5852
                     /gene="infA"
                     /locus_tag="PLW25_00810"
     CDS             5598..5852
                     /gene="infA"
                     /locus_tag="PLW25_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOS53057.1"
                     /translation="MAKEEDKIQIEGEIIEALPGTQFRVRLENGHEVLAYLSGRMRRY
                     YIRILLGDRVKVEMSPYDLTRGRIVYRQKKNEPILDEPTR"
     gene            complement(5899..>7841)
                     /gene="nifJ"
                     /locus_tag="PLW25_00815"
     CDS             complement(5899..>7841)
                     /gene="nifJ"
                     /locus_tag="PLW25_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOS53058.1"
                     /translation="FYMINANEVAAKTGMGGRVNTIMQTAFFAISGVLPREQAIDEIK
                     KAIQKTYSKRGEAVVQKNYEAVDHTLANLFEIKVPATATSKYKRRPPVPQEAPEFVVD
                     VLGPIIAFKGEELPVSAFPVDGTYPSATTRWEKRNLALEIPVWETDLCIQCGKCAFVC
                     PHAVIRQKVYDPSYLKDAPPTFKSIEAKFKEFPGMKYTIQVAPEDCTGCSLCVEACPA
                     RDKNNPLRKAINMAPQLPLREQEAINWDYFLSIPAPDPDLYQPTTVKNSQLVEPLFEF
                     SGACAGCGETPYIKLVTQLFGDRTIVANATGCSSIYGANLPTTPWAVNNQGRGPAWAN
                     SLFEDNAEFGLGMRLTLDQQNAFARELLAKMAGELGTTLVDEILNADQSTETGVRQQR
                     KRVEALKEKLATSKDANASMLLGVADALVKKSVWIIGGDGWAYDIGYGGLDHVLSSGR
                     NVNILVLDTEVYSNTGGQSSKATPLAAVAKFSAKGKGLGKKDLGLMAMSYGYVYVANV
                     AMGANDNQTLKAFLEADAYDGPALIIAYSHCIAHGIDMQKGLEQQKLLAATGLWLLFR
                     YNPTLVNEGKNPLQLDSREPTVAVKDYAYNETRYRMLVQSDENRAEMLMNEAAQFAQR
                     KWDLYKQMAAMAYSKPCEEKEQ"
     assembly_gap    7842..7941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7942..9638)
                     /gene="nifJ"
                     /locus_tag="PLW25_00820"
     CDS             complement(<7942..9638)
                     /gene="nifJ"
                     /locus_tag="PLW25_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOS53059.1"
                     /translation="MKRELITIDANEAVAYIAYRLAEVIAIYPITPSSSMGEFSDAWM
                     AAGVRNLWGTVPTVIEMQSEGGAAGAVHGALQAGALSTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVAARTVATHALSIYGDHSDVMAARQTGFAFLASDSVQTAQDQALIANAAT
                     LEARVPFVHFFDGFRTSAEINKIERLCEDDLKAMIDEKLISAVRARALSPERPVIRGT
                     AQNPDVFFQGRETANRFYARTPDIVQNAMDKFAKLTGRSYHLFDYVGAPDATEVLVLM
                     GSGAETAEETVKFLNQQGRKVGVVNVRLYRPFSVAHFIAALPATVKTIAVLDRTKEPG
                     AIGEPLYQDVVTAVNEALSTGKTPFKVAPRIIGGRYGLSSKEFTPAMVNGVFNEMVKA
                     EPKNHFTVGIVDDVTHTSLDYDHKFCIAHEKTIRCVFFGLGSDGTVGANKNSIKIIGE
                     ETNNYAQGFFYYDSKKSGSVTISHLRFGPHQIRAPYLIEKEDAQFVACHQFSFLEWLD
                     MLKYAAPGAIFLLNSVYDRDEVWDMLPIEVQQEIIEKKLKFYMINANEVAAKTGMGGR
                     "
     gene            complement(9779..10306)
                     /locus_tag="PLW25_00825"
     CDS             complement(9779..10306)
                     /locus_tag="PLW25_00825"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOS53060.1"
                     /translation="MTYTIHHESIEHRTPLFTVKRVDLSLPDGKPRVLDLIEIQNAVT
                     ILPLDSEGYIHFVKQYRIGARSELLELPAGKIENNEDPLQTAQRELREEIGMSASSWR
                     ALGNFYLSPGYASEYMYGFLAQDLVPDPLAPDADEFLNIIKVSLPDALAMVRRGEIQD
                     SKTLAVLMLAHPLLS"
     gene            complement(10435..11073)
                     /locus_tag="PLW25_00830"
     CDS             complement(10435..11073)
                     /locus_tag="PLW25_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOS53061.1"
                     /translation="MKKRILSWVILLVIGALLMTACERAATRAPRDTTRANDEIPFPV
                     STQSQIMIDILAATQTALAVEGTVESENPQALAQAQPAAMPTPMPQPEEEEPEPTPGL
                     PSTYTIQKDEFLFCIARRLNVNASEFLAINGLGVDYYAQVGDRLTIPQSGKPFDGPRA
                     LRAHPVDYTVAAGDSIGKIACTFGDVSPEAIYNANGLEVGSSLKPGQVLHIP"
     gene            11237..11686
                     /locus_tag="PLW25_00835"
     CDS             11237..11686
                     /locus_tag="PLW25_00835"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOS53062.1"
                     /translation="MKIYFSCSITGGRSEESVYQKIVSALDSMGYEVPTAHLASPEVV
                     EMEKTADPNDIFERDMAWLRECDAVIAEVSSPSHGVGYEIAYALTLGKPVLCCYQAGR
                     SVSKIITGNTSPGLSVMTYIVEEDAPVVVREFIQRLGNRPGVQESEY"
     gene            11751..12689
                     /locus_tag="PLW25_00840"
     CDS             11751..12689
                     /locus_tag="PLW25_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOS53063.1"
                     /translation="MEYKDYYQVLGVDRKATPEDIKKSFRKLAMKYHPDRNKDNKQAE
                     EKFKEINEAYEVLSDPKKRERYDQLGSSYQQWQQRGGNPGNFNWNEWYTNQGGRAQQV
                     NPEDFGDIFGGFSDFFRVIFGGMPVSGRQPTRQPGTQQSAQARRAANYEQKVQITLEE
                     AYRGTQRILQINERRIEVKIPAGARTGTKVRVAGAGPADAYGRGSDIYLVIELLPHAR
                     FKISGSDLTTEVKIDYLTAVLGGTVTVGTLSGDVVLTIPSGTQPGQKFRLSGRGMPVL
                     KSSQTYGNLYVQVKVELPKKLTDEQRKLFEQLRKHS"
     gene            12708..13880
                     /locus_tag="PLW25_00845"
     CDS             12708..13880
                     /locus_tag="PLW25_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOS53064.1"
                     /translation="MRKPVVLSIALLLLVVLMACVFIPSQDWNPSDVLENVVTKVPDI
                     VSPNVPPVFVNAEDLANVDQVLTTLYERVNPGVVTIIATTRQGEGSGSGFVYDYEGHI
                     LTNYHVVKDAETIEVDFASGKKIYGDVIATDVDSDIAVIHVDMPAAELVPLSLGDSDQ
                     LKVGQMVVAIGNPYRLTSTMTLGVVSAKGRILDSLRTTEDFTSYTAGDLIQTDAAINP
                     GNSGGPLLNLAGQVVGINRAIRTSGTTLEGGAVNTGIGYAVSINIVKRVVPILISKGS
                     YDYPLMGISSSSNELTLSMWKKISHTVTSGVYVVSVTPGGPADRAGLVGGTQATDVQG
                     FYSGGDIITAVDGLPVNIYGDLISYIFKNKSPGDVIVLTIIRNDREMEVPLTLGSR"
     gene            13925..14674
                     /locus_tag="PLW25_00850"
     CDS             13925..14674
                     /locus_tag="PLW25_00850"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53065.1"
                     /translation="MFTESSLRKIQDFSADYPVVSLYLNTEPSRGNAETHRLRLRNMI
                     KDISLKQDAELIEKFFSYTYDWSGRSVAVFSCAPADFFHAIPLAVPVKDFIQIGSKAA
                     VAPLEDLLQDYSNLGVILVDKQGARLFHFHMGELVEQQGFLGDQVKQVKAGGASSAHG
                     LRGGALDGGRAMRETIDRNQREVAETAARFFESKRVRRIMLGGTDENISRFKSHLPKS
                     MQSLVVGSFAMGMTASNAEVLQKVLDQALEK"
     gene            complement(14738..15487)
                     /locus_tag="PLW25_00855"
     CDS             complement(14738..15487)
                     /locus_tag="PLW25_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53066.1"
                     /translation="MEPQKEPQPQDSPETTEPLPGFEPATQGTSSSQEATAEPVPEVK
                     DKPVLSPSQPVKKSFWQKVLPWVIVALVFYVGGLATLFFAVYQPAKQAAAAAAASAEK
                     EITSLNSQVSQLELQNSQVQQELETTRANLVDTQDELTTAQATIEKQLVEVANANIKR
                     LAYKLMGNINTARAALEKGDTNSARQALNFASKDLSDLQVAGLTPEALSGFSNWLNEA
                     VANLEETTLQKSRAALDMLYTNLLLLVDNLP"
     gene            15613..>16328
                     /locus_tag="PLW25_00860"
     CDS             15613..>16328
                     /locus_tag="PLW25_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOS53067.1"
                     /translation="MRYMDAATLPGNTENRRIARDAGTVMLAILLGQVFSLLSSILIT
                     RAFGTGIQNEAFNASNRLPDILYQLIAGGALASAFIPTFTTLLTRGERSKAWLLASAI
                     ANIITVIFIVLGVLIALFTPWVVQRILAPGFAADPVKFALTVDLVRIQMIAPVIFGLS
                     GLGMGILNSHRSFLWPALAPMMYSIGKILGVLLLAPSLGVYGLALGVVGGALMHGLIQ
                     MPALLKLPGLKYTPTFGIRLP"
     assembly_gap    16329..16428
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16429..17396
                     /locus_tag="PLW25_00865"
     CDS             <16429..17396
                     /locus_tag="PLW25_00865"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOS53068.1"
                     /translation="LLKLPGLKYTPTFGIRLPDVREVARLMGPRVLGVAFVQLNFLVN
                     TRLASLQPLGSVTALAVGFSLMMIPEAAIAQAIAIAALPAFSAQAAAGKLEEMRNSLA
                     ALLRGLLLLALPASIGLILLRAPLVTIIFQRGEFDASSTQLVAWALLWYATGLVGHSV
                     VEIVSRAFYALHDTKTPVGVGVAAMSLNVGLSIGLSRLFNTWGWMPHGGLALANSIAT
                     FLEMGALLIFMRRRLTGLEGRHVLNGLVKALAAAGVMTLALWGWMRATAGASAWLVAL
                     GGMLVGALVYVLMLALLRVPELKLAWVGTKRRLLKRTAGESDEKQ"
     gene            17383..18135
                     /locus_tag="PLW25_00870"
     CDS             17383..18135
                     /locus_tag="PLW25_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOS53069.1"
                     /translation="MKNNSGHEFMVKTRYANISPSPQNSGVVPQPPLELALPDGAQLL
                     PLPRPADLVLAPMELRAAIEARRSIRAYAETPLSLAELTYLLWVSQGVKRVSARPSTA
                     RNVPSAGARHAFETYLLINRVAGLTPGLYRYQAIEHGLLRLETAADIAGSIAHACIDQ
                     PQLTGSAAAFIWAAVVERMFWRYVERGYRYLHLDAGHVCQNLALGAEQLGCGICPIAA
                     FDDELLNNTLGLDGEEMFVVYLATVGKKTDKK"
     gene            complement(18159..19154)
                     /gene="pfkA"
                     /locus_tag="PLW25_00875"
     CDS             complement(18159..19154)
                     /gene="pfkA"
                     /locus_tag="PLW25_00875"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOS53070.1"
                     /translation="MNTMNEPVKSIKKIAVMTSGGDAPGMNPFIRAVVRTAYANNITV
                     LGVERGYEGLIQGKFRELRIREVGGILQRGGTILQTARCTEFREAGSQREALRHINNA
                     EIDALVVAGGDGSLTGALKLAQKGVPVIGVPASIDNDIYGTDMCIGVDTALNTIVDAI
                     DKLRDTASSHNRAFLVETMGRTSGYLAVQAGIITGAELVLIPEHKTPPREVAQAIEEA
                     YRRGKTHAIVVVAEGYKPPTTELGAMIDAMDIGFTTRVTILGHVQRGGKPTAFDRMLA
                     TRFGVKAVQFLLEGKSAVMTALSGREIIPLPMEEVINHKKVIANDYIEMASMLAR"
     gene            complement(19165..22986)
                     /locus_tag="PLW25_00880"
     CDS             complement(19165..22986)
                     /locus_tag="PLW25_00880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOS53071.1"
                     /translation="MSFAHLHVHTEYSLLDGFSNIKKLIQKVKDLGMDSIAITDHGTM
                     FGVIEFFSTAVEAGVKPIIGVEAYVAARRMSDRDPQKDKHSYHLVLLAENEIGYKNLL
                     QIATAGQLEGFYYYPRIDHEFLENHKEGLIATTSCMSGEVPRTILNKGVEAGQRVLEW
                     YINTFGKDHFFIELQNHPLRELPELNRTLIDLGKRYNLRCVATNDLHYIEQEDAHLQD
                     IKLAIQTGSLLSDPNRMKMGANTFYLRSPQEMAALFPDIPSALTNTLEVAERCNVNLI
                     QKGYHLPLFTVPDGFTPQTYLKKLCEDGLQVKYPGRVDDPEVRQRLEHELKIIHEMGF
                     DAYFLIVWDLCRYAAENNVWYNTRGSGAGSIVAYTLNITLIDPLKHGLFFERFLNPSR
                     IEMPDIDLDFEDDKRSLILQYCAEKYGSDKVAQIITFGTLGARAAIRDVGRVMDVPLS
                     EVDRISKTVPAMFPEEAVTIPRSLEKCPEFQTVYSEGGYVKELIDTACRMEGVARNAG
                     THAAGVVITDKPLLEYLPLHRPTSNAEESPIKSVTQFEMNIITKLGLLKVDFLGLSTL
                     TIIHKACDLIKKRHKIELNLHNIPLDDPETFEFISQGRTAGLFQLEGSGMTRYIMQMK
                     PHNLANIIDMVALFRPGPMEKIPNYINRMHGEEEITYEHPLMEPIFKDTFGLPVYQEQ
                     LMRAVIELAGYTASEADNLRKAISKKNKNAIDEHRVKFIAGAQKNQISAVQAEEIFSG
                     WDNFARYGFNKSHAADYGVIAVETAYLKKHFPVEYMTALLSANKDESTKVAFYVADAR
                     SMGIEILPPDVNNSEWDFCIEDDPDGRTAIRFGLGAIKNVGIGPVDLIFQARQDGGFK
                     DLNDFIRRVDLHSVGKRSLECLIKVGALDRFGPRRALLEVADTLVSVSNSHFRAAECG
                     QMSIFGNATGLEDEIHLPVGVQLDRRLQLEWEKELSGMYISDHPISHYLPLIRQKATH
                     ATKELVEAEHKQKVSVAGMVTRIRSLTTKTGNQMAFATIEDLQGEVELVIFPKAWERM
                     GALVKMESVILVEGKVDLDKSDPKILVDVIKALREEDIPTDETPSDRPFAHGQEGTWQ
                     TDIDTIRYTDQAESYEEPPISEEESSWQVPTPPADHHAAPPTPDTDVVPSPATDLPQE
                     EAVSPSAQQPRENVETKFDTPPRDYILPPVIMSPIKTPPNTAAPGGTRLVTVTIKSSG
                     EKERDTRRIHRAHGLLNSFPGQDRFCFLVYEHGYRHLLDFPNHTTNATKELVEQLAEL
                     VGRENVQVENL"
     gene            complement(23188..23793)
                     /locus_tag="PLW25_00885"
     CDS             complement(23188..23793)
                     /locus_tag="PLW25_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOS53072.1"
                     /translation="MEVKFFLSDLPALRDRLLSLGAEQKQARTFEKNERFDTPDGALA
                     LSGRALRLRHDLRDILTFKGPSSIIDGVRVRPEHEVFIDNPDTARLILEGLGYEMTVR
                     YEKWRTVYLLNDLFITLDELPYGHFIEIEGADTALIRATAGALALDWDARIEASYLEL
                     FERLKQSKGLTMRNLTFADFAGITFADSDLGVRPADCPFLS"
     gene            complement(23817..24263)
                     /locus_tag="PLW25_00890"
     CDS             complement(23817..24263)
                     /locus_tag="PLW25_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53073.1"
                     /translation="MEKTTAPHPPFLLHLFASAFLLVFLINLAGFLQALRSWNWLLAA
                     GVFPHPVYLLFKTVLLALLSFTAALAIWGRRAFAPRLGQANSLLFFLWYWFDRLVLTR
                     NPLPFKNQLLPLLVSVLLLLFVLVSAWLLEPYMKETRKPPHDVPGG"
     gene            24374..24580
                     /locus_tag="PLW25_00895"
     CDS             24374..24580
                     /locus_tag="PLW25_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53074.1"
                     /translation="MKISIWDILTVVLLLTALVLLVVVMVIFIHPDSRINPFPFPSLP
                     ATIVIPTLTPTLLQMPPTWTPTPF"
     gene            24654..24974
                     /locus_tag="PLW25_00900"
     CDS             24654..24974
                     /locus_tag="PLW25_00900"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOS53075.1"
                     /translation="MSEQITKEVFDHLVGLAALQLEMEQAEYLRKQLNNQLKAIAELE
                     AIPLEKDVPISLHGVPYEASLSAPPREDEWKPYPDPAGILAQAPQVVDGYIVVPDIPH
                     TELK"
     gene            24980..26575
                     /gene="gatA"
                     /locus_tag="PLW25_00905"
     CDS             24980..26575
                     /gene="gatA"
                     /locus_tag="PLW25_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOS53076.1"
                     /translation="MEICDLNALELARLVRSRQTSAVEVVKSCLERVAQVDGRPGSLE
                     QMPLSDEDKKRVHAFITLTGENALQQAEKVDQMLAAGEDPGVLAGVPFTVKDIFCVQD
                     TLTTAASKILANFTAPYTATPVQRLQDAGAILLGKVNLDEFTYGSSNESSAYQPSPRN
                     PWNTDRVPGGSSGGSAASVAAGEAPLSLGTDTAGSIRQPAAFCGVVGVKPTYGRVSRY
                     GLIAFASSLDCPGPVARDVKDAAAMLGVIAGSDERDATAARLAVPDYLEGIEEGVKGL
                     RIGLSPDYFRITYPDPASGELLSQPLPEEVARAVNEAAEKLSTMGADIVEGVPMPNTR
                     FGIPAYFVISRVEAASNLHRYDGVKYGLRSEAPTADLKEMYKITRNQGFGPQPKLRIL
                     MGMYVSAAQYSEQYYRRALQVRTLIRQDFEAIFDPAGAYRLDALLTPTTPTTAFKMAA
                     LYGDSVLMQYADQLTVPANHAGVPGISLPAGLDNEGLPIGIQLLGADYAEKTLFRAAR
                     AYEMTTQEEDWRKQKPEVLRGRS"
     gene            26572..28038
                     /gene="gatB"
                     /locus_tag="PLW25_00910"
     CDS             26572..28038
                     /gene="gatB"
                     /locus_tag="PLW25_00910"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOS53077.1"
                     /translation="MTDYEAVIGLETHIQLNTVTKVFCSCKADSWFDPPNTNICPVCC
                     GLPGVLPVLNKAVVEKGVLLARAMNAEIRPLSFFDRKNYFYPDLPKGYQISQFDQSLA
                     RGGYLDLPIPAAASPDKQAYTRHVTIWKLHIEEDAGKTKVSNQRRLIDFNRCGVPLVE
                     MVTGPDLRTADEAAQYLIRLRQLLRWLGISEADMEKAHLRCDANVSIRVRGETKLNPK
                     TEIKNVNSIDAVREAIAREIERQIREVEAGRHIEAWTLEWDEDSGTLKKMRSKETEAD
                     YRYFREPDLLPIRLDEGWQRQILADFPELPLERRARFVEQYNLPEYDADILTSERKLS
                     DYYENTVKSYGGDPKKVSNWLMNDVLRMINDQGLNPQEMQLTPDALAEILKLVDAGTI
                     NINTGKALLQKVQESGKKPGAIVAEEGLGLVSDDSTIREVCQAVLAESPNEVAAYRAG
                     KVTLIGWFVGGVMKKMRGKADASLARQILEDLLNEKNR"
     gene            complement(28103..28582)
                     /locus_tag="PLW25_00915"
     CDS             complement(28103..28582)
                     /locus_tag="PLW25_00915"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOS53078.1"
                     /translation="MDILWSPWRMKYVTDASNSGACVFCTSPGQADSVANLIVHRGQK
                     VYAILNRYPYTSGHLMVVPFAHVSSIELLTPDVRFEMIEMVNQAILVLRKIYQPDGFN
                     IGINMGVAAGAGIAEHAHIHIVPRWNGDTSFMSTTGETRVLPEELQETFKRVSAGWP"
     gene            28703..29953
                     /gene="xseA"
                     /locus_tag="PLW25_00920"
     CDS             28703..29953
                     /gene="xseA"
                     /locus_tag="PLW25_00920"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOS53079.1"
                     /translation="MLQPPLFPVKTLSVSEITRYLRALFESDEILSDVWVQGEVSNLS
                     RPSLGHIYFTLKDAFSSIKCVIWKQTAWRLPLTLQNGMAVEAHGGVDIYERDGQYQLY
                     VDALRLTGEGALYQEFLRLKTRLEAEGLFALERKRPLPEQPSAIGLVTSSSGAALQDI
                     LNTLKRRFPLVQVILAPCAVQGEDAPPQIVKSLAALNAQNQADVIIVARGGGSLEDLW
                     AFNDEGVVRAITDSQIPVITGIGHETDFTLADFAADVRAATPTAAAEQVVPDAAEIKV
                     KLSDMANLLSATIRQICGAQRGRVQDSARRLELQSPLWRVRNDRQHIDELQARIANNQ
                     AHRLELMRSNLVHLQKRLVNLSPQAVLQRGYAIIKTAEGVLVKSVRQVHRGDVLDIRV
                     ADGAILSKVLTEPTTERNGGIADG"
     gene            29946..30176
                     /gene="xseB"
                     /locus_tag="PLW25_00925"
     CDS             29946..30176
                     /gene="xseB"
                     /locus_tag="PLW25_00925"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOS53080.1"
                     /translation="MVKSASTLSDLSYEQAFSELETIVAALESEQKPLDEALQLYERG
                     QLLVQYCAALLDQADLRIQQLNPNKETTEDAA"
     gene            30173..30622
                     /locus_tag="PLW25_00930"
     CDS             30173..30622
                     /locus_tag="PLW25_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOS53081.1"
                     /translation="MSILAGYPELIACIMGWLVSSLLKIPTYYSIHRRINLKQAFGTG
                     GMPSSHAATVTATTLAIGLFSGFDHPAFALAVAVSMIVIYDAAGVRREAGYHAVIINR
                     LIDEYVKGPLIDQKKLKEMIGHTPLEVVGGVISGTLTTLILWLIWPK"
     gene            complement(30692..32440)
                     /locus_tag="PLW25_00935"
     CDS             complement(30692..32440)
                     /locus_tag="PLW25_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS53082.1"
                     /translation="MTTADLNRTLSPSDPGDRPIGYVVGGGLKANLFVRLTLPAQEVQ
                     EGGFVVLESGNWQFYGLVTDLQLGALDPRYAELQNAARLSQPAARLLASQTLYTNLEV
                     MPALMLERGPEPGSAEYEDWAREHQGENAPRPLPIKTVPSHHAVARLASAGDVAEIFG
                     DPESKGFFIIGRTREQDHPVCINLDKFVQRSSGIFGATGTGKSFLTRIILAGLIQHNA
                     ASVLVLDMHNEYGFDDTSSDTGQAVPGLKGKYAERVRVVGLGRETTIRKRTPDLHLEI
                     YESDIQPEDIELLSRELNLRETTPTTLEALVHSFGPNRWYTEFKNMANGATIIDDEGK
                     KVPAPGSVAFWANQEGVNVAAAEGLHSKLRNVFNRPYIIERDPADKTAVNSLNNIIDT
                     LKAGKHVVLSFGDFEKDLDYLLVSNLLTRKIRESWEKMTNDFRSSGQNEPRPLVIAVE
                     EAHKILNREMASQTTFSTIAREMRKYYVTLLIIDQRPSQIYDEVMSQLGTRVSGWLGD
                     ESDIAAVLSGLAGRDSLRGMLARLQPKEEVLLLGWGVPMPILVRSRRYDNRFWEELKG
                     KNAKPDVEKSLSELGF"
     gene            complement(32455..33057)
                     /locus_tag="PLW25_00940"
     CDS             complement(32455..33057)
                     /locus_tag="PLW25_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53083.1"
                     /translation="MNAIPIGRLLRANTTSCVMGCRVSQAGTPPFGAMVLIPLSDGTR
                     IYGLVYDIHIDDDGLVRQLVSAQVSEEVLEDNRQNRNVPIEISLVFIGWEDSAGRVSH
                     ARVPRPPLTLDEIFLCSDADILRFTSNARLGYLRGLLRNQEIPAADLVTAHLVQVFKV
                     AHHPDPEGWRDRCLNEVIALLKDDYPQLMDVLSAVSVVME"
     gene            complement(33054..34163)
                     /locus_tag="PLW25_00945"
     CDS             complement(33054..34163)
                     /locus_tag="PLW25_00945"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HOS53084.1"
                     /translation="MGRDALSRAGEIRQSLEQLSVRLNENAENLKGLQRQVEEAAAKT
                     KGLRCAVPLHESLNAHFPCTLPAPASTIFAADGSQINPDPHDSVLYGLVNIGVFCIQP
                     HSGRVPLERTESHLLFGDELYASGGMASEDLVALLRDVQERRTLAQLAHLATPPVITL
                     TDGPLELYHEPRTDAAFKEKFEDYLRALDDLAIADVITAGYVSRPRADLVVRLLTLNE
                     ESSQPTVSGISDIALFHSILQPGERSAIFRLHSRSADEYKDRKALHFFYLNVGSPSRP
                     AFARVEIPQWVAENPQSVQTLHTTLVEQSALSGEAPYPYPLIRAHEIAVVKLSDRQQL
                     TTMIESELARQGILPGSRSEKQVHKDHPGRTRINP"
     gene            34370..34957
                     /locus_tag="PLW25_00950"
     CDS             34370..34957
                     /locus_tag="PLW25_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOS53085.1"
                     /translation="MRLLETTRMNSWEILLIALGLAMDCFAVSLGIGTNGMKKTARMV
                     LRLAFHFGLFQGGMAFIGWLAGRQIAGLISSFDHWLAFFLLLWVGGRMVIGSTKQEEE
                     CRKTDPTRGGSLVALSVATSIDALAVGLSLAIVDGSIFISSSIIGLTSFALSVSGYLL
                     GDKLGCRFGKRMELLGGLLLIGIGIRVLLSHLLGT"
     gene            35036..35356
                     /locus_tag="PLW25_00955"
     CDS             35036..35356
                     /locus_tag="PLW25_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOS53086.1"
                     /translation="MPVYTYRCENCGIQFDQTQKFSDPALTRCPECGKKSLKKVYTPV
                     GIVFKGSGFYATDHRSPSGAAHNSNTKHEEAKSTEKTETKTETKAASQTTVESTSTPS
                     GDKA"
     gene            35509..35658
                     /locus_tag="PLW25_00960"
     CDS             35509..35658
                     /locus_tag="PLW25_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53087.1"
                     /translation="MAKPNKQTGAQKRSKITSSQRKMRAQQIILAVIGIVVIISMILA
                     LTMKF"
     gene            35666..37237
                     /locus_tag="PLW25_00965"
     CDS             35666..37237
                     /locus_tag="PLW25_00965"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HOS53088.1"
                     /translation="MPKKQKWFWLAALIAAWLFDFLFWSKGSGVSFFIWIAILLLAGY
                     TLAWSEGKKPSALSVLLTCFILGFAAVIALRNEPLTRAVSILLTLNGMLLLTATFLNG
                     YWPWYRIKDYVVELVRVIGAGFSGAIWLLAKGKTPPPVDGSPAKSGWSKAWPVIRGLL
                     IALPVVAVLGLLLASADQVFSDWVKRILNIEKLPEYLFRLFYILIIAGFLVGVYLKAI
                     YPRKTAQKPEPNQAWLQPFLGWTESGIVLGAVNLLFIIFVIIQVRYLFGGEANISETG
                     FTYAEYARRGFSELVAVAVLSLGLYLLLGTVTKTESQGSKVGFTALAVLLMVNVLVIL
                     ASSFLRLMLYENAYGFSRPRTYTHIFIYWLAGLILAVMVLELMKRRGRIALALLVAVV
                     GFGATMAILNIDGFIVQRNVARAEAGAELDVVHLTTLSADAVPQLLQKYQSPELRKEV
                     REGLGATLACMEKAIQDPADLPWQEYNFGQARAAQLLRENADSWSQYKAYQTDRDWVV
                     RLNGQRIYCAYFIMD"
     gene            complement(37303..37674)
                     /locus_tag="PLW25_00970"
     CDS             complement(37303..37674)
                     /locus_tag="PLW25_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOS53089.1"
                     /translation="MEEEPRLYELIYAIVRQVPRGRVATYGQIARIAGRCSAQMVGFA
                     LAALPVYRGRDIAEEVPWQRIINAQGKISPRGFGMGSQRQRVLLEEEGILFDDQGSID
                     LSVFGWDGVLFSPVKNELDLI"
     gene            37741..38796
                     /locus_tag="PLW25_00975"
     CDS             37741..38796
                     /locus_tag="PLW25_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOS53090.1"
                     /translation="MKRWQFWVGLLISVAFLYFAVRGLNLADFWKAVKDANYWWILPG
                     VAVFFAGLWVRAWRWHYLLGPVKKIPTRIMFPITCIGYMGNNIYPARAGEVLRAVVLK
                     RREKVPISASLATIIVERIFDGVVMLAFVFLNLPALAKVSSFESGFVGNIQNLALIGT
                     AIFLAALLVFLLAAMFPRTTARLGGWFIERLLPPRAREKTTGIMLKFLDGLESLRSPF
                     NILMVFFTSVIIWLLETVKYWFVMQAFTFSVSFFALMLLNGIANLATTIPSAPGYIGT
                     WEAVTRAVLVAFGVPGAEALGYGVVLHVALWLPVTLVGAYLMTREGIRWSDSLRQEAR
                     SGEAEAGGEICDKESNK"
     gene            38793..40085
                     /locus_tag="PLW25_00980"
     CDS             38793..40085
                     /locus_tag="PLW25_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOS53091.1"
                     /translation="MKIAVIGAGFGGLAAAYDLVKAGNTVTLYEAESTPGGLAAGFKE
                     PDWEWSVEKYYHHWFTSDEHMFRLFRELGVEDRVMILSPKTVMYHKGKFYPFDSITSA
                     LLYPGLGYGLNKIRFGLVGLYLRFTNRWQPMEQTTVAEWMKKWAGEKVYTQMWEPLVM
                     GKFSEKYANQVNMAWMWARLHARTTRLATYQGGFQAFADEFAAILAKMGVKFAYRTVV
                     KQLMSEKEGGLTLSTTHGNETYDRALVTISPALLSKMAPQLPEDYLKGLLDLKSMGAV
                     VMTLALKHQLSREGYYWYNLPKDAGFPFLALVEHTNFLSPQKFGGNHIIYCGDYLDPA
                     HEYFQLSKEELLQRFTPSLKKINPNFETGWITGSWLVKTNYAQPVPLLNHSRHIPSIE
                     TPLKGLYFASMSQVYPWDRGTNFAVEIGRRAADLMLRA"
     gene            40606..41949
                     /gene="dnaA"
                     /locus_tag="PLW25_00985"
     CDS             40606..41949
                     /gene="dnaA"
                     /locus_tag="PLW25_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOS53092.1"
                     /translation="MDHRNAWQAAVGQLQMEMSRASYETWVQPAELVRYENDLFTVGV
                     PNAYACDWLKSRLTAKLNSILSGIMECPQKVEFIVWHKDYENQPQTASTAEPLPLISP
                     AVTGSQPVVNHRYNFDNFVVGSSNRLAHAACLAVAENPAQAYNPLFLYGGVGLGKTHL
                     LHAIGNFAQARGEQVLYVSSEEFTNDLINAIRTHTTQAFRDRYRRIDVLLVDDIQFIA
                     GKESTQEEFFHTFNTLHGQDKQIVISSDRPPKAMNTLEERLRSRFEWGLTADIQPPDL
                     ETRIAILRSKAERSRHAVPPAILEIIARQFQSNIRELEGALTRVVAYADLRGRPLSEE
                     LVNVALTDLLPQRNAFEPAHVVNVVASAFGITPEKLMGRDRTRAAALPRQVVMYLLRQ
                     EANVSLPQIGEAMGGRDHTTVMYACEKVADMIERDDRLRRQVLQIREQLYGRKTT"
     gene            complement(41995..42846)
                     /locus_tag="PLW25_00990"
     CDS             complement(41995..42846)
                     /locus_tag="PLW25_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53093.1"
                     /translation="MSQTSSKTRLFLAILAVVVGVFMVGVAPFLIQTSLDRVMTALVK
                     VSAQKPAYASGILLFSVLYPLYRAIIFIAGITLIVIARSIREGKEWTFPVGLLASAFP
                     ASGGMFMMLPYVSFVPGLPLPMLVSLTGLLFFLCLILLRKVNTQKKIAQLLTLTFCGI
                     MTAHAFVIAVGGMRQTLTRLEKPLYSGIEGWVLAWSSPVQFICVILLFVAIYKIAARK
                     MEGWWMALVAAVSLVAMNTPTQIIRTLRTDATSIDYLIGALLSTALLVILLLPKIKRA
                     LFEEPAS"
BASE COUNT         9751 a        11002 c        12214 g         9847 t
ORIGIN      
        1 gggcgactcc ctgggagagt aggtcgttgc caagagactt ttttctatta acgccaatcc
       61 accgtggagc gctgtcgcgg tggttttttt gttctctggc ggctctccat gataaaaaaa
      121 aaataaaaaa ccaaaaaaaa cctccggaag agtggactcc cggaggtttt ttttccactc
      181 gttataaaat acgctgcaaa tgctggctat aacgtccttc gttcccgatc gtagttctga
      241 cccaggaaag ccggaacgcg cgacttaccg aaggtgaaga tcatcacgat gatcgtggcg
      301 atgtacggca gcattgagag gaactggtag gggatgaagg aaccgcctac ctggaagcgg
      361 aacgacagca cctcgatggc ggcgaagaac agcgagccga tgaggatggg cagcgggctc
      421 caggcgccga agaaggtcag cgcgatggcg atccagccgc gacccttggt catcaagtca
      481 gtgaaagtgc cggtgtatac catgggaagg taagcgcccg ccaggccgat gagcatcccg
      541 ccgatgatgg aggtgatgta gcggttggaa accacgttga tacccagcgc gtctgaggct
      601 ttggggtttt cacctaccga acgcaggcgc aagcccatgt tggtacggaa caatatgtac
      661 cacaggaata tcgaaagcag gatcgagaaa tagaccagga tgttctgctt gaagagcacc
      721 ggcccgatga tggggatctg gctgagcagg gggatgggga tttcatgcag tgtctttacc
      781 agcggcgggg tgagagtgat gccgatgacg agcttgggta aaagagaaga taacccggta
      841 ccaataaaga acagcgccaa gcccacgaca aactggtcca tctttaacgt ggtggtgaag
      901 tatgccaaaa ccaagccaaa gaggccgcct gccgccatag ccaccagcgc accgattagg
      961 ttactgccag tggtgtaact caccagaaag cccacagaag cgccaacaag catgattcct
     1021 tcctgcgcca cattgaacac cccggtacga ccgccgagca ttgtgccctg gcttgccagt
     1081 acaaatggga ccatggacag gaaagtgagt ttgaggaaat cagtgatggt tgtttctaaa
     1141 ccgctcattt ctgcctcctc ttgatgatgt cagagagaag aataaagatc aggatcaacg
     1201 cttctgcgat cagcacgatc tgcaccggca ccatgatggt acgctgcatg gcgctgccgc
     1261 ctacttcaag aatggaaata aagaatgcca cgaagggaac tgcctggttc ttgccgcgcg
     1321 ccatcaggcc gatgatcagc ccgagggtcg ggaagttgga gtgcaacccc tcgatgaggc
     1381 ggtggtgggt gccggccgct tcgataaccc ccgccaatcc agcaacggcc gccccgatga
     1441 ccagtgaaaa caggaacatc tttcgcgtat caatgccgtg cactttggaa gcacgcgggt
     1501 tggcccctgt taccagcaat tcataaccag aagtcgtttt actggtgaag atgtacaccg
     1561 caatcgccac gatgacggcc aggatgatgc cggaatgcag cggagggttt ttcaccagca
     1621 caggtaaaaa gccgttcgcg ccaattggta tcgtggtggg gtgcccagcc gcgggatcgc
     1681 gccagggacc ggtggctaca aagtcgacca gcgcaaacga aatgaagttc aacagcacgg
     1741 tcgagagaat ctcgttgatg ttgaactggt agagcagcca ggctgcgatc aaaccccaga
     1801 aagaaccagc cagcataccc gcgaggatca ccagcggcag caacaacgcc gggggcaggt
     1861 ctttaaaaag aatgccaacc gcggtagcgc cgatggcgcc aaccagcatc tgtccttcgg
     1921 caccgatatt gaacttacct gatgataagg gaacggtaaa agccagcgcg gtgagcgtca
     1981 gcgggatcca ctttaaaagg gtttggacaa acccgttggg cgtgcggaag gaggtgaaga
     2041 gaatggtctg ataggctttt atcgggttaa aacccagggc ccataacagt acccctacaa
     2101 ccaaaaaagc cgccaggaca gagaccaggt aaggaaagaa ttttttaatt tgtgtcatag
     2161 tatacgctct ctccctttat ttacttttct gctcgtaacc gctcatcagg cgaccaatct
     2221 cgtacttctc gaacttctca cgggggagaa tccctactac tttaccgcgg tacataacgg
     2281 cgatgcggtt gcaaatgagc agcagttcat cgaggtcttc agaaaggagc aggattgctg
     2341 tgccggtggc ggaatttccg aggagcttct ggtagacaaa atccatggaa ggaatatcca
     2401 ggccccgggt gggattgtgg gcgaccatca acctggaggg ctgcgagaag gccctggcga
     2461 tcatgacgcg ctggatattg ccccctgata agttaccgcc tatttcatcc ggtccagtgg
     2521 tcttgatgtt gtactcgctg atcatcttcc tggaattctc atgcacgttc ttccagttga
     2581 tgatcccttt tttgctatac ggttcaaggt tgtgatagcc caaaatgagg ttatgcgcaa
     2641 cgctggcttt gtttaagaac ccatcagaaa ggcgatcttc agggacatat acgaaacctt
     2701 cagcgatacg ttctttggta ctgtgagtgg tgatctcttg accattgaag ttgattttac
     2761 cgctggtaac cggctgaacc cccatgacca cttcagccag ttcacgctga ccgttaccag
     2821 ccacgcccgc cactccgaaa atttctccag aaccgatgct gaaggagata tcggtcaact
     2881 gctggatctt gtgctgactg atcagagaaa cgccttccag gctcagcact tcttgactgc
     2941 cggcaacagt tgcgctgtcc tcgtgtgagg tgaacatcac gcttttttcc aggtccatct
     3001 tatcgccgac catggcacgc accagcgctt catcgctggc ttcttttcgc gtcaggctca
     3061 ccacattttt cccctcgcgc agcacggtga tacggtcaca aattgacagc acctcgcgca
     3121 gtttgtgggt gatgaacacg atgctcaacc cttcgcgcac catgattttt aaggtttcaa
     3181 agagagagtc gacctcctgg ggaatgaggt tggtggtggg ctcatcgagg atcaggatgt
     3241 taacgccgcg gtacagcgct tttaagattt caactttctg gcgggttccc ataggcaggg
     3301 tttcaacacg cgccgaaagg tcaatttcaa ggccaaaacg tttacaaagt tccttgatcg
     3361 ccgcttcttc tacctggtaa ttggcgctta aaggatggga gactttggaa cccaggatga
     3421 tgttctcaag cactgtgtaa ttattgatct gtaaaaaatg ttggtgcacc atgccgattc
     3481 cttggcggat ggcgtctctg ggtgagtgta tggttactgg ttgccctttc agcagaattg
     3541 tcccggaatc cgcacggtaa agaccataaa gaatattcat caaggtggtc tttcctgcgc
     3601 cgtttccgcc cagtaaaccc aggatctctt tcggttttaa ttcaaaatcg acattacaca
     3661 gggcaaccac cctatcaaag gatttgttga cctgttgcat ctgaagcaca ttttcttgtt
     3721 ccatattccc gtcctgttca gagggtttta gcaacttccg ggggagcttc aagcatgatg
     3781 tttcggctcg accccggaag ttatcgtctc aacttatttc aacaagggtt atttggcgga
     3841 ggtaacttcg gtagaatcct ttacaaccac gatttctccg ctaatgactt tgttgatgac
     3901 ctcttcaacc ttggcggcga cctcgggggt cacgttctgc agcgggagct gcagcgctac
     3961 accggtgtcg aaccctaacg ggtagtaacc acccgtttta ccgccttcaa tgctggtgat
     4021 gatatcaacc agtggcttgg agaagtcgta cagcacggca gtgatatagt tatcggggcc
     4081 gaagctggtt ttgtcggagt atttggcggt gatgaggatc ttcttgtcag tagtggcttt
     4141 ggcggcttcg aaaacaccca ccatgcccag gttcacagaa cccatgatga cgtcaacgcc
     4201 gtcagcgatg agcgcgtcag ccagctggcg agtcttggcc gggtcattga agtcagccgt
     4261 ccacacagga acgagctcaa ccttgatatc caggtcgctg atagcctgct ggatggcgtg
     4321 tacttcctgg taggagaagg gaagggcctg cccgccgagg taggcgatct ttccggtctg
     4381 tgaggccagg gctgcagcgg cgccgatacc gtagaagcca acattgaagt tgcggtcaat
     4441 gaaccacagg ttcggtgaag ggtcagcgat gggtgcgtca ccttcggcaa tgaacacaac
     4501 ctcggggaag agcttggcga gttcagcggt ctggttcaca aactgaccac cgtgggtcca
     4561 aatgacgttg aaccccgcgt caatgtattc gcgcattaca cggtcaacat caggcacagg
     4621 aacgctttcg gagtataccg cctggattcc gagttgttcc tgcgcagcgg tcatgcccac
     4681 ataagccagg gtgttgtaat cgttatccgt aatcacaccc gggaagatag cagccagctt
     4741 gacctccatg gctggttcct gcgccgcggg ggcttcggta gccgctgggg cttcggtctc
     4801 ttgaactgcg gggggttctg tggcaactgg cgccgcggtg ggcttagcgc atgcagagag
     4861 caatacgctc aaaaccagca aaacagagat ggcaaacaac aggttctttt tcatttcttc
     4921 tcctttttga tattggtggg attttctccc actggcatca agccgcttac attttacccg
     4981 aattacgccg tttttattcc tgcttaattt cccgatatct caatgacata taccctgttt
     5041 aaaaacagcg agggagaaat ttgctcattc aaaataacct gtttttcgtc cctgtgcgcc
     5101 aaataatgaa agggagtaac ctgatcaatg tccataactg cctgtaaacg acgggcaaaa
     5161 aaagattgac agagaccttg cactcccgtt ataattacgc ttgcagttgg ttagaaaaac
     5221 caaagaactt tcaaagaggt gatgaccgaa aactcgatac atccgcgaaa gaattctgga
     5281 ggaaggacac tgatcgactg cgtgacttta cagtcgattt tattttcccg gcgaagggag
     5341 gtgagaaacc atggcttctg ttgttttgcg tcccaatgaa tcacaggatt cactgctgaa
     5401 acgcttccgc aagaaggtag tgaagagcgg cgttttaagc accattcgcc gcaagcgctg
     5461 gtttgtttcc aagagcgaac ttcgccgcgt agaaaagaaa aaggccatca ggcgtatcaa
     5521 gcgccgccag tatacacggg tgggcgaata gcctgcttga cgaacgagca gcccgagagg
     5581 ctgggatagg aggaattatg gccaaagaag aagataagat acagatagaa ggcgagatca
     5641 tcgaagcgct gcccggaacc cagttccgcg tacgcctcga gaacggccat gaggtactcg
     5701 cttatctttc cgggcggatg cgcaggtatt acatccgcat tttgctgggc gatcgcgtta
     5761 aagtggagat gagcccgtac gacctgaccc gcggacgcat cgtttaccgg cagaagaaga
     5821 acgaacccat actggatgaa ccaaccagat aaaaaagacc ggtcttttat tcaaagaccg
     5881 gtctttttgt ttgggcgatt attgctcttt ttcctcacac ggtttgctgt aggccatggc
     5941 agccatttgc ttgtacaggt cccatttacg ttgcgcgaat tgcgccgctt cgttcatgag
     6001 catttcagcg cgattctcat cgctttgtac cagcatgcgg taacgggttt cgttataggc
     6061 gtaatccttt accgccacgg tgggctcgcg gctgtcgagc tgcagcgggt tctttccttc
     6121 gttcaccagg gtggggttgt agcggaacag cagccacaaa ccagtagcgg caagcagttt
     6181 ttgctgttcg aggcccttct gcatatcaat gccgtgcgcg atacagtggc tgtaagcgat
     6241 aatgagggca ggtccgtcat aggcatctgc ctcgaggaag gctttgaggg tctggttgtc
     6301 attggcgccc atcgccacgt tagcgacgta aacgtaaccg taagacatgg ccatcaaccc
     6361 caagtctttt ttgccaagac ccttgccttt ggcggagaat tttgccaccg ccgccaatgg
     6421 cgtggctttt gaggattgac cgccagtgtt ggagtagact tctgtatcca gcaccaggat
     6481 gttgacgttg cgccctgaag aaagcacatg gtcaagacca ccatagccaa tatcgtaagc
     6541 ccaaccgtca ccgccaatga tccacacgct cttctttacc agcgcgtctg ccacacccag
     6601 cagcatcgaa gcgttggcat ctttactggt cgccagtttt tcttttaatg cttccacgcg
     6661 tttgcgctgc tggcggacgc cagtttcagt ggattggtca gcgttaagga tttcatccac
     6721 cagggttgta cccagctcgc ctgccatttt agcgagcagc tcacgcgcga aggcgttctg
     6781 ctggtccagc gtcaggcgca taccgagacc gaactcggcg ttgtcttcaa acaacgagtt
     6841 tgcccaggcg ggtccgcgcc cctggttgtt taccgcccag ggcgtggtgg gcaggttagc
     6901 gccgtagatg gaagaacagc cggtggcgtt ggcaacgatg gtacggtcac caaacaattg
     6961 ggtgaccaat ttaatgtagg gcgtttcacc gcaaccggcg caggcgccgg agaactcaaa
     7021 caggggttcg accagttgcg agttcttgac cgttgtgggt tggtaaaggt cggggtcagg
     7081 tgctgggatg gagaggaagt agtcccagtt tatggcttcc tgttcacgca gagggagttg
     7141 gggcgccatg ttgatggctt tacgcagcgg gttgttctta tctcgcgccg ggcaggcctc
     7201 aacacacagg ctgcaacctg tgcaatcttc tggcgccacc tggatggtgt acttcatgcc
     7261 ggggaattct ttgaacttgg cttcgatgga cttgaaggtt ggcggcgcgt ctttgaggta
     7321 agaggggtca tacacctttt ggcggatgac ggcgtgcggg caaacgaaag cgcacttacc
     7381 gcactgaatg cacaggtcgg tttcccacac ggggatctca agagcgaggt tgcgcttttc
     7441 ccagcgcgtg gtggcggagg ggtaagtgcc gtctacgggg aaggcgctga ccggcagttc
     7501 ctcgcctttg aaggcaatga tgggaccgag aacgtcgacc acgaattccg gagcctcttg
     7561 cggcactgga gggcggcgtt tgtacttgct ggtagcggtg gcgggtactt ttatctcgaa
     7621 caggttggcc agggtatggt caaccgcttc gtaattcttt tgcaccacag cctctccacg
     7681 cttgctgtag gtcttttgga tagctttttt gatttcatcg atagcctgct cgcgcggcag
     7741 cacaccgctg atggcaaaaa aggcggtttg catgatggta ttcacacgcc cgcccatgcc
     7801 cgttttcgcg gcaacctcgt tggcgttgat catgtagaat tnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn ncgcccgccc atgcccgttt tcgcggcaac ctcgttggcg
     7981 ttgatcatgt agaatttgag cttcttctca ataatctcct gctgcacctc gatgggcagc
     8041 atatcccata cttcatcgcg gtcatagacg ctgttgagca ggaagatggc gccaggagcg
     8101 gcgtatttga gcatatccag ccattccaaa aaactaaact ggtggcaggc aacaaattgg
     8161 gcgtcttcct tttcaatgag gtaaggagcg cgaatctggt gaggcccaaa gcgcagatgc
     8221 gagatagtga ccgagccgga ttttttcgaa tcatagtaga agaatccctg cgcgtagtta
     8281 ttggtctctt cgccgatgat cttgatggag ttcttgttgg cgccgacggt cccgtcagaa
     8341 cccaggccaa agaacacgca gcggatggtc ttttcatgag cgatgcagaa cttgtggtca
     8401 tagtctaggc tggtatgagt gacgtcatcg acaataccca cggtgaagtg gttcttcggc
     8461 tcagctttga ccatttcgtt gaaaacgccg ttcaccatgg ctggggtgaa ctctttggag
     8521 gagagcccgt agcgcccgcc aatgatgcgc ggggcgactt tgaaaggagt tttgccggtg
     8581 gagagggctt cattaacagc ggtgaccaca tcctggtaga gcggttcgcc aatggcgccg
     8641 ggttccttgg ttcggtcgag cacggcgatg gtcttgactg tggcgggcag cgcggcaata
     8701 aaatgcgcca cggagaacgg gcggtataag cgcacgttga ccacgccaac cttgcgaccc
     8761 tgttggttaa ggaacttcac tgtctcttca gcggtttcag cgcctgaacc cataaggacc
     8821 agcacctcgg tggcgtctgg cgcgccgaca taatcaaaga ggtggtaact tcgcccagtg
     8881 agtttggcaa atttatccat ggcattctga acgatgtcag gcgtgcgcgc gtaaaaacgg
     8941 ttggctgttt cgcgcccctg gaagaaaaca tcagggttct gggctgtacc gcgaatgacc
     9001 gggcgctcgg gtgaaagcgc gcgcgcgcga acagcggaaa tgagtttttc atcgatcatg
     9061 gctttgaggt cgtcttcgca caggcgctcg atcttgttga tctcagcgga ggtgcggaag
     9121 ccgtcaaaga aatgcacaaa aggcacacgc gcttcgaggg tggcggcgtt ggcgatcaac
     9181 gcctggtcct gcgcggtctg cacagaatct gaagccagga aggcaaagcc tgtctggcgg
     9241 gcggccatta catcagaatg atcgccatag attgaaagcg cgtgcgtggc gaccgtgcgc
     9301 gctgccacat ggaatacagt ggaggtgagc tcgccggcga ttttgtacat gttggggatc
     9361 atcaacaaca gaccctgcga agcggtaaag gtggtgctca gggcgccggc ttgcagcgcg
     9421 ccgtgcactg cgcctgccgc gccgccctct gattgcattt ctatcacagt tggtactgta
     9481 ccccacaggt tacgcactcc cgcagccatc catgcgtcgg agaattctcc catgctcgag
     9541 gagggcgtga taggataaat cgcgatgact tccgccaggc ggtaggcaat atacgccacc
     9601 gcttcgttgg cgtctatggt gatcaattct cttttcattt tcaactccaa tcggttggtt
     9661 ataggcgttc agccttaatc gtcagcgatg ccgccaagat tataatacat aaacactccg
     9721 aattcccccg ttcaaccacc ttcgggttga atcgagtgcc atttcaaacg accaaacctc
     9781 aggacagcaa aggatgcgcg agcataagga cagccagggt tttactgtcc tgtatctctc
     9841 cacgtctgac catagcgagc gcatccggca gcgatacttt gatgatatta aggaactcgt
     9901 ccgcatcagg cgcaagcggg tcagggacaa gatcttgcgc cagaaaacca tacatatatt
     9961 cactggcgta acctggcgaa aggtagaagt tgccgagagc ccgccacgag ctggctgaca
    10021 tcccaatttc ttcgcgcagt tcacgctgcg cagtttgcag cgggtcttca ttattctcga
    10081 tcttaccggc aggcagctcc agcagttcag accgggcgcc aatgcggtat tgtttaacaa
    10141 agtgaatata cccctcgctg tccagcggaa ggatggtgac tgcgttctga atttcgatga
    10201 ggtcaagaac acgcggcttg ccatcaggca gggataggtc gacgcgtttg acagtgaaca
    10261 aaggggtgcg atgttcgatg gattcgtgat ggatggtata ggtcatcgga ttggacctcg
    10321 caagaaggat catttgggta tggtgtggtt gaccgcgctt aattattatg gcacagaata
    10381 agaaaaaggc tttcctgaaa ccaggaaagc cttgtggatc accagatggg gaaatcaggg
    10441 aatatgcagc acctgaccgg gtttcaaaga agaacccact tccaggccat tggcgttata
    10501 gatggcttca gggctgacat ctccaaaagt acaggcgatt tttccgatgg aatcaccggc
    10561 ggcaaccgtg taatctacag gatgggcgcg cagcgcgcga ggtccatcaa aaggcttacc
    10621 gctttgcggg atggtgagcc ggtcgcctac ctgggcgtag taatcaaccc ccaacccgtt
    10681 gatggcgaga aactcgctgg cattaacgtt cagtcgtcgc gcgatgcaga acaagaattc
    10741 atctttttga atggtatagg tggacggcag gccgggggtg ggctcaggtt cctcttcttc
    10801 tggctggggc atgggggttg gcatggctgc gggttgtgct tgggcaagag cctgggggtt
    10861 ttcgctttcg actgtgcctt caaccgccag cgccgtttgc gtggccgcca gaatatcgat
    10921 cataatttgg gattgagtgg atacgggaaa ggggatttcg tcgtttgctc ttgtggtatc
    10981 ccgtggggcg cgggtggcag cgcgttcgca cgctgtcatt aacagggcgc caatgaccag
    11041 gagtattacc cagctgagga ttcgtttttt catcttgcat ctccttatcc ttcccgcgtg
    11101 tcagggtagt gtgcatcatt aaaacgcgcg gtgtgataat atgtgtgggg atgccgcggc
    11161 agtcaagacg cgcgccacac ggttatcgtc tggataaatt acgttgcaat ccttatgcca
    11221 attagacgga gtggtgatga agatttattt ttcttgttca attaccggtg ggcgatctga
    11281 agaatccgtt taccaaaaaa ttgtgagcgc gctggattcc atgggatatg aagtgccaac
    11341 tgcgcacctg gcgtcgccgg aagtggtaga gatggaaaaa acggcagacc ctaatgacat
    11401 ttttgaacgt gatatggcgt ggttgaggga gtgcgacgca gtgattgcgg aagtgtcttc
    11461 tccttcacat ggtgtggggt acgagatcgc ttatgcatta acgctgggta aaccggtgtt
    11521 gtgttgttac caggcgggca ggagtgtctc taaaatcatt accgggaata ccagccctgg
    11581 cttatccgtg atgacatata ttgtggagga agacgcacct gtggtggtga gggaatttat
    11641 tcagcgattg ggcaaccgac ctggcgtgca agagagcgaa tattagcgtt ctattagaga
    11701 ccggttcgca actattgact tttatcttcg acaaagaata caattaaacc atggagtata
    11761 aggattacta tcaggttctg ggtgtggacc gcaaggccac cccggaagac atcaagaaat
    11821 cttttcgcaa gctggctatg aagtatcatc ctgatagaaa taaggataac aagcaagcgg
    11881 aagaaaaatt caaagaaatc aacgaagcat acgaagtgct gagtgatccc aagaaacgcg
    11941 agcggtatga tcagctgggc agttcatacc aacaatggca gcaacgcggc ggaaaccctg
    12001 gcaacttcaa ctggaacgag tggtacacca accagggcgg gcgcgcacag caagtgaacc
    12061 cggaggactt tggcgatatc tttggtggtt tttccgattt tttcagggtg attttcggcg
    12121 gcatgccggt aagtgggcgg cagccaaccc gccaacccgg cactcaacag tctgcgcagg
    12181 cgcgccgggc ggctaactac gagcaaaaag ttcaaatcac cctggaagaa gcctaccgtg
    12241 gcactcagcg tattctccag atcaatgaac ggcgcatcga ggtaaaaatt ccagcggggg
    12301 cgcgcaccgg cacaaaagtg cgtgtggcgg gcgctggccc agcggatgcc tacggtcgcg
    12361 gctctgacat ttatctggtg atcgagctgc tgccgcacgc gcgctttaaa atcagcgggt
    12421 ctgacctgac gacggaagtg aaaattgatt acctcaccgc tgtgctgggc ggcacggtaa
    12481 cagtgggcac actttccgga gacgtggtgc tcaccatccc ctcgggcacc cagccgggcc
    12541 aaaaattccg cctgagcggc aggggtatgc cggtgctgaa gtcttcacag acctatggca
    12601 acctttacgt gcaggtgaaa gtggaactgc ccaagaaatt aaccgatgaa cagcggaagc
    12661 tgtttgaaca actgcggaag cattcctaga aataagaagg tgatcgaatg agaaaacctg
    12721 ttgtcttgtc cattgcgttg cttttattgg tggtgctaat ggcgtgtgtg ttcattccca
    12781 gccaggattg gaacccatca gacgtgttag agaacgtcgt caccaaggtg ccagatattg
    12841 tttcaccaaa tgtgccgcct gtttttgtta acgcggaaga cctcgccaat gttgaccagg
    12901 tgttaaccac cctttacgaa agggtaaacc ctggcgtggt aaccatcatc gccaccacca
    12961 gacaagggga gggaagcggt tcaggatttg tgtatgatta cgaaggacat atcctcacca
    13021 attaccacgt ggtgaaagac gctgagacga ttgaagtgga tttcgccagc ggtaaaaaaa
    13081 tctatgggga tgtgattgcc acggatgtcg attctgacat cgcggttatc catgtggata
    13141 tgcccgctgc tgaactggtg ccactttctc ttggagattc agaccagtta aaagtagggc
    13201 aaatggttgt ggctattggc aacccatacc ggctcaccag caccatgacc ctcggggtgg
    13261 tgagcgccaa agggcgcatt ctggattcat tacgcactac ggaagatttc acttcataca
    13321 cagccggcga ccttattcaa acggacgcag cgatcaaccc cggtaattcg ggcggaccgt
    13381 tattaaacct ggcgggccag gtggtgggca tcaaccgcgc catccgcacc tcaggcacca
    13441 ccctcgaagg aggggcggtg aacaccggta tcggttacgc ggtttcgatc aatatcgtca
    13501 aacgcgtggt gccgattctg atcagtaagg gttcgtatga ttatccgttg atgggtattt
    13561 caagctcatc caacgaacta acgttgagca tgtggaaaaa gatcagtcat acggtcactt
    13621 caggggttta tgtggtcagt gtcaccccgg gaggcccggc tgaccgggcg gggttggtcg
    13681 gcggcaccca ggcgacggat gtgcagggat tttacagcgg gggagacatt atcaccgctg
    13741 tggacggctt gccggtgaac atttacggtg atcttatcag ctatatcttc aagaacaaat
    13801 cgccaggaga tgtgatcgtt ctaaccatca tccgcaatga tcgtgaaatg gaggtgccgc
    13861 tcaccctggg ttcgcgataa caacgaactg ctggtcaaat aaaaaaactt aagaggaggt
    13921 atcaatgttc acagagtcca gtctgcgaaa gatccaagat ttttcagccg attacccggt
    13981 cgtcagcctg tatttgaaca cagaaccaag ccgtggtaac gcggaaacac accggcttcg
    14041 actgcgtaac atgattaaag atatttccct caaacaagat gctgaactca ttgaaaagtt
    14101 cttcagttac acttatgact ggtcaggtcg ctcggtagcg gttttttcgt gtgcgccggc
    14161 cgactttttt catgccatcc cgctggctgt accggtgaaa gattttatcc agatcggttc
    14221 aaaagcggcg gtagcgccgc tggaagacct gctgcaggat tacagcaacc tgggggtgat
    14281 cctggtggat aaacaaggcg ccaggctttt tcattttcac atgggagaat tggtggagca
    14341 gcaggggttt ttgggcgacc aggtcaagca agtgaaagca ggtggagctt cttcggcgca
    14401 tggcttgcgc ggcggggcgt tggatggcgg gcgcgcgatg cgcgagacca ttgaccgcaa
    14461 ccaacgtgaa gtggctgaaa cagcggccag gttttttgag agcaaacggg ttcggcggat
    14521 tatgctcggt ggaacggatg agaacatctc gcgctttaag agccacctgc caaaatcgat
    14581 gcagagcctg gtagtgggta gtttcgcgat gggcatgacc gccagcaacg ccgaagtgtt
    14641 gcagaaagtg ctggatcagg cgctggaaaa gtaaaaaagt gaaaaataac agccgcggtg
    14701 ttggagatga acaccgcggc tgtttttatt gataaggtca gggaaggttg tcaaccagca
    14761 ataacaggtt ggtatacagc atatccaggg cggcgcgcga tttttgaagc gtggtttctt
    14821 cgaggttcgc aactgcttca ttcagccagt tagagaaacc cgagagggct tctggcgtca
    14881 acccggccac ttgcaaatcg ctcaggtctt tactggcaaa gttcaacgcc tggcgcgccg
    14941 aattcgtatc gcccttctcc agcgccgcgc gggcggtgtt gatgttgccc atcaacttgt
    15001 aagccagccg tttgatattg gcgttggcaa cttcaaccag ttgtttttca atggttgcct
    15061 gcgcagtggt taactcatcc tgcgtatcca ccaggttggc gcgcgtggtc tccagctctt
    15121 gttgcacctg ggaattctgc agttcaagct gcgatacctg gctgttcagc gatgtgattt
    15181 ctttttcagc gctggccgcc gcggctgcag cggcttgctt ggctggctgg taaaccgcaa
    15241 agaagagtgt tgccagcccg ccaacgtaga aaacaagcgc cacaatcacc cagggcaaaa
    15301 ctttttgcca aaagctcttt ttaaccggct gagaaggcga aagaacaggt ttgtctttca
    15361 cctctggtac aggttcagcg gttgcctcct gtgaagatga ggttccttgg gtggcgggtt
    15421 caaaacccgg taaaggttct gtggtttcag ggctatcctg gggttggggt tccttttggg
    15481 gttccatggg tttgtccttt cgtagcatgg tgtgtgaatt acaggggaag tatatcattc
    15541 taaccacgct tgtgcgccat gtgtgatgaa agaaagacat atccgaatgg ccaagccgga
    15601 catagggtta taatgcgtta tatggatgct gcgacattac cgggtaatac cgaaaatcgc
    15661 cgtatcgccc gcgacgcagg cacggtgatg ctggctatcc tgctggggca ggtgttcagc
    15721 ttgctttcat ccattctcat cacccgtgct tttggcacag ggatccaaaa tgaagccttt
    15781 aacgcctcca accggctacc tgacatcctt taccagttaa ttgccggcgg agcgctggca
    15841 tccgctttca tccccacgtt caccacgctg ctcacgcgcg gcgaacgctc aaaagcctgg
    15901 ctgctggctt ctgccattgc caatatcatc acggtcatct ttattgtgct gggggtgctc
    15961 atcgctctct ttactccctg ggtggtgcag cgtatcctgg cgcccggttt tgccgctgac
    16021 ccggtcaagt tcgcgctgac cgttgacctg gtgcgcatcc agatgatcgc gccggtcatc
    16081 ttcggtttaa gcgggctggg gatgggtatt ctcaacagtc accgcagctt tttatggccg
    16141 gcgctggcgc cgatgatgta ttccatcggc aagatcctgg gggtgctgct gctggcccca
    16201 tcgttgggcg tatatggcct agcgctgggc gtggttggcg gagcgctgat gcatgggctg
    16261 attcaaatgc cggcgctgct gaagctgcca ggcctgaagt acacgccaac ttttggcatc
    16321 aggctgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ctgctgaagc
    16441 tgccaggcct gaagtacacg ccaacttttg gcatcaggct gcccgatgta cgcgaggtgg
    16501 cgcggctgat gggaccccgt gtgttaggcg tagcatttgt gcagctgaac tttctggtga
    16561 acacccgcct ggcatccttg cagccgctgg gcagcgtgac ggcgctggcg gtgggctttt
    16621 cattgatgat gatccctgag gcagccatcg cccaggcgat cgccatcgcc gcgctgcccg
    16681 ccttctcagc gcaagcggca gccggaaaac tggaagaaat gcgcaattcg ctggctgcgc
    16741 tgctgcgagg gctgctgttg ctggcactgc ccgcttctat cgggttgatc ctgttgcgcg
    16801 cgccgctggt aacgattatt ttccaacgcg gcgagtttga tgcgtcatcc acgcagctgg
    16861 tggcgtgggc tttactgtgg tacgccaccg ggctggtggg gcattcagtg gtagaaatcg
    16921 tttcacgcgc gttttatgcg ctgcatgata ccaaaacacc tgtgggggtg ggcgtggcag
    16981 cgatgagcct taacgtgggg ttaagcattg ggttaagccg gctgttcaac acctggggtt
    17041 ggatgccgca cggcggattg gcgctggcaa actccatcgc cacctttctg gaaatgggcg
    17101 cgttattgat ttttatgcgc cgccggctta cagggttaga aggaaggcat gtgcttaatg
    17161 gtctggtaaa agcgctcgcc gcggcaggtg tgatgacgct ggcgctgtgg ggctggatgc
    17221 gcgccaccgc tggcgcttcc gcgtggttgg tcgccctggg tggtatgctc gtgggtgcac
    17281 tggtttatgt gctgatgctt gcgttgttgc gcgtacctga gctgaagctg gcgtgggttg
    17341 gaacaaaacg aagattgttg aaaagaacag caggagaatc agatgaaaaa caatagcggt
    17401 catgaattca tggtgaaaac tcgttacgcc aacatcagcc cctcaccaca gaatagcgga
    17461 gtggtaccgc agccgccgct cgagctggcg ctgcctgacg gcgcgcagtt gctgccgctg
    17521 ccgagaccag ctgacctggt gctggctccc atggaattga gagcggcaat tgaagcccgg
    17581 cgcagcatcc gggcttatgc tgaaacacct ctatcgctcg cagaactcac ctacctgttg
    17641 tgggtatcac agggggtcaa acgtgtttct gccagaccct ctactgcgcg caacgtgcct
    17701 tctgctggcg cgcgtcacgc tttcgagacc tacctgctga ttaaccgggt ggcgggatta
    17761 acccccggct tgtatcgcta ccaggcaatc gaacacggat tactgagatt ggaaacagcc
    17821 gctgatatcg cggggagcat cgcgcacgcc tgcattgacc agccgcagct cactggcagc
    17881 gccgcggctt tcatttgggc ggcggtggtg gagcgcatgt tctggcgtta cgtcgagcgt
    17941 ggttaccgct atctgcatct cgatgccggt catgtttgtc agaacctggc gttgggcgcg
    18001 gagcagctgg gctgcggtat ttgccccatc gccgcttttg atgatgaact gctcaacaac
    18061 acgctcgggc tggacggcga ggagatgttc gtggtttacc tggcgacagt ggggaagaaa
    18121 acggataaaa aataaccgtt ctcaggagcg cagcgaggtc atcttgccag catactggcc
    18181 atctcaatgt aatcattggc gatgaccttt ttgtggttta tgacctcttc cattggaaga
    18241 ggtatgatct cgcggcccga taaagcggtc atcaccgcgc tcttgccttc caaaaggaac
    18301 tgaaccgctt taaccccaaa gcgcgtggcc agcatacggt caaaggcggt gggcttgccg
    18361 ccgcgctgca catgcccaag tatggtgacc cgagttgtaa aaccgatatc catggcgtcg
    18421 atcatggcgc cgagctccgt ggtaggcggc ttgtaacctt cagccacaac cacgatggca
    18481 tgggtcttcc ctcgacggta ggcttcttca atggcttgtg cgacctcccg gggaggtgtt
    18541 ttatgctcag ggatgagcac cagttcagcg ccggtgatga ttcctgcctg caccgccagg
    18601 tacccggagg tgcgccccat tgtctcaacc agaaaggcgc ggttgtgaga tgaggcggta
    18661 tcgcgcagtt tgtcgatggc atctacgatg gtgttgagcg cggtatcgac gccgatacac
    18721 atgtcggtgc cgtaaatgtc gttatcgatg ctcgccggca cgccgatgac cggcactccc
    18781 ttctgcgcca gttttagcgc gccggtcagc gagccgtctc cgccggcgac gacgagcgcg
    18841 tctatctcag cgttattgat gtgacggagg gcttctcgct gactgccggc ttcacgaaac
    18901 tctgtgcagc gcgcggtttg aagaatggtt ccccctcgct gaagaatacc gcccacttcg
    18961 cggattctca actcgcggaa cttgccttga ataagtcctt cgtaaccacg ttcaaccccc
    19021 aggacggtaa tgttgttggc ataggcggtg cgtacaacgg cgcggatgaa agggttcatc
    19081 cccggggcat cacctccaga ggtcattacg gcgatctttt tgatactttt caccggttcg
    19141 ttcattgtgt tcatcttctc ttgtttacag gttttcaacc tgaacgttct ctctgcctac
    19201 cagctcagcc aactgctcga ccagttcttt ggtagcgttg gtcgtgtggt tcggaaaatc
    19261 cagcaagtgc cggtaaccgt gctcatacac caaaaaacag aaacggtctt gacccgggaa
    19321 agaattgagc aggccatgcg cccggtggat tcggcgtgtg tctcgttcct tttcaccact
    19381 ggatttgatg gttaccgtca ctaacctggt tccaccgggc gctgctgtgt tgggcggtgt
    19441 ttttattggc gacatgatca ctggcggaag gatgtaatcg cggggtggcg tatcaaattt
    19501 ggtctcaacg ttttcgcggg gttgctgcgc tgacggagaa acggcttcct cctgcggcag
    19561 gtcagtcgca ggggagggca ccacatctgt atcaggcgtg ggaggcgccg cgtggtgatc
    19621 cgcagggggt gtaggaacct gccaggagga ttcttcttct gagatcggtg gctcttcata
    19681 actctctgcc tggtctgtat agcgtatggt gtcaatatcg gtttgccagg taccttcctg
    19741 cccgtgagcg aaaggacggt ctgaaggcgt ttcgtcagtc gggatatcct cctcgcgcaa
    19801 ggctttgatc acatccacca gtattttggg gtcagattta tccaggtcca ccttgccttc
    19861 caccaggata acggattcca ttttgaccaa cgcgcccatt ctttcccagg ctttggggaa
    19921 gatgaccagt tcaacctcac cctgcaggtc ttcaatggtg gcaaaggcca tctggttgcc
    19981 cgtcttggtg gttagcgaac ggatacgggt gaccataccg gcaacactga ccttttgttt
    20041 gtgctcggct tcaaccagtt ccttggtggc gtgagtggct ttctggcgga taagcggcag
    20101 ataatgagag atggggtgat cggatatata catacccgac agttcttttt cccattcgag
    20161 ttgcaggcgg cggtccagtt gaacgccaac gggcaaatga atctcatctt ccaaaccggt
    20221 cgcgttgcca aagatgctca tctgaccgca ttccgcagca cggaagtgac tgttactgac
    20281 tgaaacgagg gtgtctgcca cttccagcag agcccgcctc gggccaaaac gatccaacgc
    20341 gccaaccttg atcaggcatt ccaggctgcg tttgcccacg ctgtgcaggt cgacacggcg
    20401 aataaaatcg ttaaggtcct tgaagccgcc gtcctgcctg gcctggaaga tcaaatcaac
    20461 cggaccaatt cccacatttt taatcgctcc cagcccgaag cgtatggctg tcctgccatc
    20521 cgggtcgtct tctatacaaa aatcccattc gctgttgtta acatcaggcg gcaagatctc
    20581 gatgcccatc gagcgggcat ctgccacgta aaacgccacc ttagtgctct catctttatt
    20641 ggcagagagc agcgcggtca tgtattccac cgggaaatgc ttcttcaggt aagccgtttc
    20701 gaccgcgata acgccgtaat cggcagcgtg gcttttattg aaaccatagc gggcgaaatt
    20761 atcccacccg gagaagattt cttctgcttg cacagccgaa atctgattct tctgagcgcc
    20821 ggcgataaat tttacgcggt gctcatcaat agcgtttttg ttcttcttgg agatggcttt
    20881 acgcaggtta tctgcttctg aagccgtata gccagccagt tctatcacgg ccctcattaa
    20941 ctgttcctgg tataccggta aaccgaaggt atctttgaat attggctcca tcaatggatg
    21001 ctcgtaggtg atctcctctt caccgtgcat gcgattgatg tagttgggaa ttttttccat
    21061 gggcccgggc cgaaaaagcg ccaccatgtc gatgatattc gccaggttgt gaggtttcat
    21121 ctgcatgatg tatcgggtca tgccgctgcc ttccagctgg aacaggccgg cggtgcgccc
    21181 ctggctgata aactcgaatg tctccgggtc atccagggga atattgtgca ggttgagctc
    21241 gatcttgtgg cgctttttga tcaggtcaca ggctttgtgg atgatcgtga gggtggaaag
    21301 gcctaaaaaa tccaccttga gcagacccag cttggtgatg atgttcatct cgaactgggt
    21361 gaccgacttg atgggagatt cctcggcgtt actggttggg cggtgcagcg gcaggtactc
    21421 cagcagcggc ttgtcggtga tgaccactcc ggcagcgtgt gtgccggcgt tgcgggccac
    21481 cccttccatg cggcaggcgg tatcaatgag ttctttcaca tatccgccct cgctgtaaac
    21541 cgtctggaac tcagggcatt tttccagcga cctggggatg gtcaccgctt cctcggggaa
    21601 catcgccgga acggtctttg aaatgcggtc gacctctgaa agcggtacat ccatcacacg
    21661 accgacatca cggatggcgg cgcgcgcccc aagggtacca aaggtgatga tttgcgccac
    21721 cttatcgctg ccgtattttt cagcgcagta ttgtaaaatc agggaacgtt tgtcgtcctc
    21781 gaagtcaagg tcgatgtctg gcatttcgat gcgagacggg ttgagaaagc gctcaaaaaa
    21841 caaaccgtgc ttgagggggt cgatgagggt gatgttgagc gtgtaggcca cgatcgaacc
    21901 cgcgccagac ccgcgggtgt tgtaccacac gttattttca gcagcgtagc ggcacagatc
    21961 ccacacgatc aaaaaatacg catcaaagcc catttcgtgg atgatcttca gttcgtgttc
    22021 aaggcgttgc cttacttcgg ggtcatcgac gcgcccagga tatttcacct gtaaaccgtc
    22081 ttcacagagt tttttgagat acgtttgcgg ggtgaatcca tcgggtacgg taaacaaagg
    22141 cagatggtag cccttctgga tgaggtttac attgcaacgt tcagccactt caagggtatt
    22201 ggtcaaggct gaagggatgt ctggaaagag cgctgccatt tcttgcggtg agcgtagata
    22261 gaaggtgttc gcgcccatct tcatgcggtt tgggtctgag agcagtgacc ccgtttgaat
    22321 ggcgagctta atatcttgca ggtgcgcgtc ttcctgttca atgtaatgca aatcgttggt
    22381 ggccacgcac ctgaggttat agcgtttacc caggtcaatt aatgtgcggt tcaactccgg
    22441 cagttcgcgc aggggatggt tttgcaactc gatgaagaaa tggtctttac cgaaggtgtt
    22501 gatataccat tcgagcactc tctgcccggc ttccactcct ttgttgagga tcgtacgcgg
    22561 aacctcgcca gacatacacg aggttgtggc gattaaacct tctttgtggt tttcaaggaa
    22621 ctcgtggtca atacgcggat agtaataaaa cccttccagc tgcccggcgg tagcgatttg
    22681 caacaggttt ttataaccaa tttcgttttc agccaacaag accaggtgat acgagtgctt
    22741 gtctttctga ggatcgcggt cgctcatacg gcgggcggcc acatacgctt cgacgccaat
    22801 gatgggtttt accccagcct cgaccgcggt ggaaaaaaac tcaatgaccc cgaacatcgt
    22861 gccgtggtca gtgatggcaa tcgaatccat gcctaaatct ttaacttttt ggatcagctt
    22921 cttgatgttg ctgaagccat cgaggagaga atattcagta tgaacatgaa ggtgggcaaa
    22981 agacatgaca gaccgcggtc tcgctctcta ctgataatta ttaaaaatat tatagcatgt
    23041 ggcgtttctc cgtttcttaa ggatcattta tccccgtgta attttaagaa agcgcgagcc
    23101 cccgcgcggc gaaaaggctc aagtagcggt gcgagaccgt aacgaagcaa acaagccaaa
    23161 aaattgaaaa cggaccaggc tgatttgtta tgagagaaaa gggcaatcgg ctgggcgtac
    23221 gcctaggtcg ctgtcggcga acgtgatacc agcgaagtca gcgaaagtca gattgcgcat
    23281 ggtgagcccc ttgctttgtt taagcctctc gaagagttca aggtaactgg cttcaatacg
    23341 ggcgtcccag tccaaagcga gcgcgccggc tgtggcgcgg atgagggctg tgtcggcacc
    23401 ttcaatctca atgaaatgac catagggcag ttcatccagg gtgatgaaaa ggtcgttaag
    23461 gaggtaaacc gttcgccact tttcgtagcg cacggtcatt tcgtatccca gaccttcgag
    23521 gatcaggcgc gccgtgtccg ggttgtctat gaacacttcg tgttcgggtc tgacgcgcac
    23581 gccgtctata atagaggaag gacctttgaa ggtgagaata tcccgcaggt catggcgcag
    23641 gcgcaacgcc cgaccagaaa gggcaagcgc gccatccgga gtgtcgaagc gctcgttttt
    23701 ctcgaaggtt cgcgcctgct tctgttcagc cccaagcgag agcaacctgt ctcgcaaagc
    23761 gggcaggtca gacaggaaaa atttcacttc catttccacg ccgggttttt ccagggtcaa
    23821 cctccaggta catcatgtgg tggcttgcgg gtttccttca tgtagggttc cagcaaccat
    23881 gctgatacta acacaaataa caacagcagc acagatacca ggagcggaag gagctggttt
    23941 ttaaacggca gcgggttacg agtgagcacg agacggtcaa accagtacca caaaaaaaac
    24001 agcagactgt ttgcctggcc cagtcttggc gcgaacgccc tgcgccccca gatggcaagc
    24061 gctgcggtga aggataaaag agcgagcaac actgtcttaa aaaggaggta gaccgggtga
    24121 ggaaagaccc ccgctgccag cagccagttc caggagcgca gcgcctgcaa aaagcctgcg
    24181 agattgatca gaaacacaag aagaaaagcg ctggcgaaca gatgcagcaa aaaaggaggg
    24241 tggggcgcgg tcgtcttttc catcacaggt gttgtggatt atagcacgcg cccttctggg
    24301 cagcgccaac ttgacagccg tttaaagtcg gatatactga caaagacttc gcgagaaaaa
    24361 gaaaaaggcg cggatgaaaa tctcaatctg ggatatcctg acggtcgtgt tgctgcttac
    24421 tgctctcgtg ctgctggtgg tggtgatggt gattttcatc catcctgatt ctcgcatcaa
    24481 cccgttccct tttccctcgc tcccggcgac gattgtaatc cccacgctta cccccaccct
    24541 gttgcaaatg ccgcccacct ggacgcccac ccccttttag gagagaatcc aggttctcat
    24601 tccttcaccc tgaacgagat ttcttctacc cgacacaaag agagagttga cctatgagcg
    24661 aacagataac aaaagaggtt tttgaccatc tggttggatt ggccgcgctg caacttgaga
    24721 tggaacaggc tgaatacctg cgtaagcagt taaacaacca gttaaaagcc attgctgaac
    24781 tggaagccat cccgctggaa aaggatgtgc ccatcagttt gcacggggtg ccttatgaag
    24841 catcgctcag cgccccgccg cgcgaggatg agtggaaacc ttatcctgac ccggcaggca
    24901 ttctggcaca ggcgccgcag gtggtggatg gctacattgt ggttcctgat atccctcata
    24961 ccgagttaaa gtaggtcgta tggaaatttg tgatctcaac gcacttgaat tggcgcgcct
    25021 ggtgcgcagc cggcaaacct ccgcggtgga ggtggtgaaa tcctgccttg aacgcgttgc
    25081 ccaggtagac ggaagaccgg gttcgctgga gcaaatgccg ctatccgatg aagataaaaa
    25141 acgcgtgcac gccttcatca cactgactgg tgaaaacgcg ctgcagcagg cggagaaagt
    25201 ggaccaaatg cttgccgctg gcgaagatcc gggcgtgctc gctggtgtgc cgttcacagt
    25261 aaaagacatt ttttgcgtac aggataccct cacaacagcg gcttcaaaaa tcctggcgaa
    25321 ctttacggct ccctacacag ccaccccggt gcagcgcctg caggatgctg gcgccatact
    25381 gctgggaaag gtaaacctgg atgaatttac gtatggttct tccaatgaat cctccgccta
    25441 tcaacccagc ccgcgcaacc cctggaatac cgaccgcgtg cctgggggct cctcaggggg
    25501 cagcgccgcc agcgtggccg ctggtgaagc gccgctctct ttgggcactg acactgccgg
    25561 atctatccgt caacccgcgg cgttttgcgg agtggtgggg gtcaaaccaa cctatgggcg
    25621 ggtttcgcgc tatgggttga tcgcttttgc ctcctcgctc gattgccccg ggccagtggc
    25681 gcgcgacgtg aaagatgctg ccgccatgct gggggtcatt gccggcagtg acgagcgcga
    25741 tgccaccgcc gcgcggctgg cagtgcctga ttatctggaa ggcattgaag agggagtgaa
    25801 ggggctgcgc attggcttgt cgccggatta ttttcgtatt acctaccccg acccggcgag
    25861 cggagagctg ctcagccagc cgctgcctga agaagttgcc cgcgcagtga atgaagcggc
    25921 tgaaaaactg tcaacgatgg gagcggatat tgtggagggc gtacccatgc ccaacacacg
    25981 ttttggcatc ccggcgtact tcgttatcag ccgtgttgaa gccgcttcca acctgcaccg
    26041 gtatgacggg gtgaagtacg ggctgcgcag tgaagcgccc accgcagact tgaaagagat
    26101 gtataaaata acgcgcaacc aggggtttgg tccgcaaccc aaactgcgca tcctcatggg
    26161 tatgtatgtg agcgccgcgc aatatagtga acaatattac cgtcgcgcgc tgcaagtgcg
    26221 cacgcttatc cgccaggact ttgaggcgat atttgacccg gctggcgcgt acagactgga
    26281 cgcgctgctg acacctacca cgcccaccac tgccttcaag atggcagcgt tgtatggaga
    26341 ctccgtgctg atgcagtacg ctgaccagtt gacggtgccc gccaaccacg ccggcgtgcc
    26401 gggtatctcg ctgcctgccg ggctggataa cgaggggttg cccattggca tccagctgct
    26461 tggcgccgat tatgcagaga aaacgctgtt ccgcgcagcg cgggcatacg aaatgaccac
    26521 gcaagaggaa gattggcgta aacaaaaacc tgaagtgctg cgagggcgat catgacagat
    26581 tacgaagccg tcatcgggct tgaaacccac attcaattga acacggtcac caaggtgttc
    26641 tgctcatgta aagccgactc atggtttgac ccgcctaaca ccaatatttg cccggtgtgc
    26701 tgcgggctgc ccggggtgct gccagtgctt aacaaagcgg tggtggagaa aggcgtgttg
    26761 ctggcgcggg cgatgaacgc cgagatccgt ccgctctctt tcttcgaccg taaaaattat
    26821 ttctaccctg atctgcccaa gggctaccag atctcgcaat tcgatcagtc gttggccagg
    26881 ggaggatacc tggacctgcc gataccagcc gcggcctcgc ctgacaagca agcgtacacc
    26941 aggcacgtga ccatatggaa attgcatata gaagaagacg ccggcaagac caaggtaagc
    27001 aaccagcggc gtctgattga ctttaatcgc tgcggcgtcc cgctggttga aatggtcacc
    27061 gggcctgacc tgcgcactgc cgacgaagcc gcgcagtatc tcattcgcct gcgccagttg
    27121 ttacgctggc tgggtatctc cgaagccgat atggaaaaag cgcacctgag atgcgacgcc
    27181 aatgtatcca tccgtgtgcg gggtgaaacc aagctgaacc cgaaaacgga gatcaagaac
    27241 gtcaattcca ttgacgcagt cagagaggcg attgcgcggg aaattgagcg ccagattcgt
    27301 gaggtagagg caggcaggca tatagaagcc tggacgctgg aatgggacga agatagcgga
    27361 acactgaaaa agatgcgttc caaagaaaca gaagccgatt accgctactt tcgtgagcct
    27421 gacctgctgc ccatccgcct ggatgaggga tggcaaagac agattttggc ggactttcct
    27481 gagctgccgc tggaacgacg cgcgcgcttt gtggagcaat ataatctacc tgaatatgat
    27541 gctgacatcc tcaccagcga gcgcaaactc tcagattatt atgaaaacac ggtgaaatca
    27601 tacggcggcg atcccaaaaa ggtctctaac tggttgatga acgatgtgct gcgcatgatc
    27661 aacgatcaag ggttgaaccc gcaagagatg caactgaccc ctgacgcgct ggcggagata
    27721 ttgaaactag tcgacgcggg taccattaat atcaacaccg ggaaggcatt actgcaaaaa
    27781 gtgcaggaga gcggtaaaaa acccggtgct attgtggcgg aggaggggtt gggactggtg
    27841 agcgatgaca gcaccatccg cgaggtgtgc caggcggtac tggcagagag tcccaatgaa
    27901 gtcgcagcgt accgcgcggg gaaggtgacg ctgattggct ggtttgtggg cggagtgatg
    27961 aaaaagatgc gcggcaaggc agacgcctcc ctggcgcgcc aaattttgga agacctgtta
    28021 aacgagaaaa atcgctaaga gagcgaacac aaacgcccat cccccaaaag ccattcttgg
    28081 gcgagggttt gttcgcagcg gtttatggcc agccagccga tacgcgctta aaggtctctt
    28141 gcaactcttc tggtaacacc ctggtttcac ccgtggtcga cataaaactg gtgtcgccgt
    28201 tccagcgcgg gacgatgtgt atatgcgcgt gttcggcgat gcctgcgcct gccgccacgc
    28261 ccatgttaat gccgatatta aagccatctg gctggtagat cttgcgcagc accaggatgg
    28321 cctgattgac catttcaatc atttcaaaac gcacatcggg ggtgagcagt tcaatactgg
    28381 aaacgtgcgc gaagggcacc accatcaggt gcccgctggt gtaggggtat cgattgagaa
    28441 tggcgtagac cttttgaccg cggtgcacaa tgaggttggc aacgctgtct gcttgtccgg
    28501 gcgaggtgca aaacacgcac gcgccggagt tacttgcgtc ggtaacgtac ttcatgcgcc
    28561 agggagacca gagaatgtcc atttttccgc cttctttctt ttactggctt aattctacac
    28621 gcgatatgag cattttttag acccgcttaa aaacacgcct gttctgttga caggtgctca
    28681 aggtcgatgt tatcctatcc ctatgttgca accacccctg ttcccggtga agacgctcag
    28741 cgtcagcgag atcacgcgtt acctgcgtgc gctctttgag tctgacgaga tcctgagcga
    28801 tgtgtgggta cagggagaag tgtccaacct ttcgcgaccc tccttagggc atatttactt
    28861 tacgctaaaa gatgcttttt cttcaataaa atgtgttatc tggaaacaaa ccgcatggcg
    28921 gctgcctttg acgcttcaaa acggtatggc agtggaagcg catggcgggg tagacatcta
    28981 tgagcgcgat gggcagtacc agctctatgt ggatgcgctg cgcctgaccg gggaaggcgc
    29041 gctttaccag gaatttctgc gtcttaaaac ccgcctagaa gccgaaggat tattcgctct
    29101 tgaaagaaag cgcccgttac ctgaacaacc gtctgctatc gggctggtca cctcttccag
    29161 cggagcggcg ctgcaggata tattgaatac attgaagcgg cgctttccgc tggttcaggt
    29221 tattctggcg ccctgcgcgg tgcagggaga agacgcccct ccgcagattg tgaaatcgct
    29281 ggcagccctg aacgcgcaaa accaggccga tgtcatcatt gtggccagag gcgggggttc
    29341 cctcgaggat ttgtgggcat ttaatgacga aggagtcgtg cgcgccatca cggattcgca
    29401 aataccagtg atcactggta ttgggcatga gacagatttt acccttgctg atttcgctgc
    29461 agatgtccgc gctgccacac ccaccgccgc ggcagaacag gtggtgcccg acgcggctga
    29521 gatcaaggtg aaattgagcg atatggccaa tctgctgtcc gccaccatcc gacagatctg
    29581 cggcgcacag cgtggtcgcg ttcaggatag cgccaggcgg ctggagctgc aatctccgct
    29641 gtggcgggtg cgcaatgacc gtcagcatat tgatgaatta caggcgagga tcgctaataa
    29701 ccaggcgcat aggcttgagc tgatgcgcag caacctggtt catttacaaa agcggctggt
    29761 taatctcagc ccgcaggcgg tattgcagcg gggctacgcc atcattaaaa cagcagaggg
    29821 tgtattggtg aagagcgtac gccaggttca ccgcggggat gtgctcgata tccgtgttgc
    29881 cgatggcgcg attctttcaa aagtgctaac cgagcccaca acagaacgta acggagggat
    29941 tgccgatggt taaatcagca tcaacgcttt cagacttgag ttatgaacag gcgttcagtg
    30001 aacttgaaac cattgtcgcc gcgctggaaa gcgaacaaaa accgctggac gaagcattgc
    30061 aactttacga acgcggtcaa ctgctggttc aatattgcgc cgccctgctc gaccaggctg
    30121 acctgcgcat tcaacagctt aaccctaaca aagaaaccac agaggacgct gcgtgagcat
    30181 tctggcgggt taccccgagc tgattgcctg tatcatgggg tggctggtct cttccctttt
    30241 aaaaataccg acctattact ctatacaccg gcgcatcaac ctgaagcaag cttttggcac
    30301 tggcggcatg cccagctctc acgcggcgac ggtgacggcg accacactgg ctattggcct
    30361 gttcagcggg ttcgaccacc cggccttcgc gttagcggtg gcggtctcga tgatcgttat
    30421 atacgatgcc gcaggggtaa ggcgcgaagc cggttaccac gcggtcatta tcaaccgcct
    30481 aatcgatgaa tacgtgaagg gccccctgat agaccagaaa aagttgaagg agatgatcgg
    30541 tcataccccg ttggaagtag tgggaggtgt catttcaggg accctgacga cgttaattct
    30601 ctggctcatc tggcccaagt gatgcctgcc gatggcaggc acttctctga aaacgcatcc
    30661 gtctttttaa tatgctctgg tctacgatcg cttaaaagcc aagttctgaa aggctttttt
    30721 ctacatcggg tttcgcgttt ttccccttca gttcctccca gaaccggttg tcatagcggc
    30781 gagagcgcac gaggatgggc atgggcaccc cccaccccag caggagcact tcctctttgg
    30841 gctgcaggcg tgccagcata ccgcgcaacg agtctcgccc ggccaaacca gatagcacag
    30901 cggcgatatc actctcgtct cccagccagc cagaaacacg cgtgcctaac tgcgacatca
    30961 cctcatcgta gatttgcgaa gggcgctggt cgatgatgag caaggtgacg tagtatttgc
    31021 gcatttcacg cgcgatggtg ctgaaagtgg tctgtgaggc catttcgcgg tttaagattt
    31081 tatgcgcttc ctccacagca attaccagcg ggcgcggttc gttttgcccg ctgctgcgaa
    31141 aatcgttggt catcttttcc caggattcac ggatcttgcg tgtaagcagg ttggaaacca
    31201 gcaggtaatc caggtctttt tcaaagtccc caaaggagag cacgacgtgc ttgccagcct
    31261 tgagtgtatc gatgatgttg tttaacgaat tgaccgcggt cttgtctgcg gggtcgcgtt
    31321 cgatgatata aggacggtta aaaacgttgc gcagcttgct atgtaagcct tctgccgccg
    31381 ccacgttcac cccttcctgg ttggcccaaa aagccacgct gccgggggcg gggacttttt
    31441 tcccttcatc atctatgatc gtcgccccat tagccatatt cttaaactcg gtataccagc
    31501 ggttgggccc aaatgagtgc accagcgcct caagggtggt gggggtggtc tctctgagat
    31561 ttaattcacg ggagagcagc tcgatatctt caggctggat atcagattca tagatctcaa
    31621 ggtgcaggtc tggggtgcgc ttgcggatgg tggtttcacg ccccagcccg accaccctga
    31681 cgcgctcagc gtatttgcct ttgagccccg gaaccgcctg cccggtgtca gaggaagtat
    31741 catcgaaacc gtactcgtta tgcatatcca gcaccagcac agaggcggcg ttgtgttgaa
    31801 tgagaccggc caggatgatg cgcgtgagga aagattttcc ggttccagtg gcgccaaaga
    31861 tgcccgaact gcgctgcaca aacttatcca ggttgatgca taccgggtgg tcctgttcac
    31921 gcgtgcgccc aataataaag aagcccttcg attccgggtc accaaagatt tcagcaacat
    31981 caccggcgga tgccagcctg gcaacggcgt gatgcgaggg aacagtcttg ataggcagcg
    32041 gacggggggc attttctccc tggtgttcgc gcgcccagtc ctcgtattca gcagaccccg
    32101 gctcaggccc gcgctcgagc attaaggcgg gcatcacctc gaggttggtg taaagcgttt
    32161 ggctggccag cagacgggcg gcgggttgcg aaaggcgcgc ggcattctgc agttcagcgt
    32221 agcgcgggtc aagcgcgccc agctgcaggt cagtgaccag gccgtagaac tgccagttac
    32281 cgctttcgag taccacaaac ccgccctcct gcacctcctg cgcgggcagg gtaagccgaa
    32341 caaagaggtt ggctttgagt ccgccaccaa ccacgtaacc aatagggcgg tcgccagggt
    32401 ctgatgggga aagcgtgcgg ttaagatcag cggttgtcat ttttttacct ctttctattc
    32461 catcaccact gagactgcgc tcaaaacgtc catcaactga ggataatcat ctttgagcag
    32521 agcgataact tcgttcaggc agcggtcacg ccaaccctcg gggtctgggt gatgcgccac
    32581 tttgaacacc tgcacgaggt gagcggtcac caggtcagcg gcaggtattt cctggttacg
    32641 cagtaggccg cgcaggtaac ccagcctggc attgctggtg aagcgcagga tatctgcatc
    32701 actgcataag aatatttcat ccagtgtcag cggagggcgg ggcacgcggg catgcgaaac
    32761 ccgaccggcg ctatcctccc agccaatgaa taccaggctg atctcgatgg gcacgttgcg
    32821 gttctggcgg ttatcctcca gcacttcctc ggaaacctgc gcgctgacca gctggcgcac
    32881 cagaccgtca tcatcgatgt gaatgtcgta caccaagccg taaatgcgtg tgccatcgct
    32941 caaagggatg agcaccattg ctccaaaagg gggagtacca gcctggctga cacggcagcc
    33001 catcacacaa ctggtcgtgt tggctcgcag taatcgtcca ataggtatcg cgttcatggg
    33061 ttgatcctcg ttcttcctgg atggtcttta tgcacctgtt tttcagacct gctgccgggc
    33121 aggatgccct gacgggcaag ttcgctttcg atcatcgtgg tcaattgctg ccggtcgctt
    33181 aactttacga cagcaatctc atgggcgcgg atgagcggat aggggtaagg cgcctcgccg
    33241 ctcagggcgg attgttccac cagtgtggtg tgcagggtct gcaccgattg cggattttcc
    33301 gctacccact gggggatttc cacgcgagcg aaagcagggc gagaggggct gcccacgttg
    33361 aggtagaaaa aatgcagcgc cttacggtct ttatactcat ccgcagagcg cgagtgcagg
    33421 cggaaaattg cagagcgctc gccaggctgc aggatgctgt gaaaaagggc aatgtcgctg
    33481 atgccgctga cagtcggttg gctgctctct tcgttgagcg taagcagcct gaccaccagg
    33541 tcggcacgcg gccggctgac gtagccggcg gtgatcacat ccgcaattgc caggtcgtct
    33601 agcgcgcgca ggtagtcttc gaacttctct ttaaaggcag cgtcagtccg cggttcatga
    33661 tacagttcga gcggtccgtc agtaagggtg atcacaggcg gggtggcgag gtgcgctaat
    33721 tgcgccaggg tgcggcgttc ctgcacgtcg cgcagtaatg ccaccaggtc ttctgaagcc
    33781 attcctccgg aggcgtacag ttcatctcca aataaaagat gcgattcagt ccgctccagc
    33841 ggtacgcgcc cgctgtgcgg ttggatacaa aacacaccga tgttgaccag cccataaagc
    33901 acagaatcat gcgggtcagg gttgatttgc gaaccgtctg ccgcaaaaat ggtgctggcg
    33961 ggcgcgggca gggtgcaagg aaagtgcgcg ttgagagatt catgcagcgg aacagcgcag
    34021 cgcagccctt tggtttttgc cgcggcttcc tccacctggc gctgtaaacc cttcaggttt
    34081 tcagcatttt catttaatcg gacggataac tgttcaaggc tttgacgtat ttctcccgcg
    34141 cgggataaag catctctgcc catatcgcgt atctgctgac tgaggagaag gtaattaacc
    34201 ggcacgtaac ccctttaaat aatcgaacat ttgtttgaat tatacataat tcaatcgcca
    34261 acggcaagcg gttatgggga ttcgaacctt aaaaattagc aaacactgga gcaatgacag
    34321 tgattaccat ggcatacagc catgcctgat tctcgcaact ggatgtataa tgcgccttct
    34381 ggaaaccacg cgcatgaata gttgggaaat tctcttgatt gcgttaggcc tggcgatgga
    34441 ttgttttgcg gtatcgctcg gcatcggcac aaatgggatg aaaaaaaccg ccagaatggt
    34501 attgcggctg gcgttccatt ttggcttatt ccagggtggt atggcgttca tcggctggct
    34561 ggctgggcgt cagatcgcgg gcttgatttc cagttttgac cattggctgg ctttcttttt
    34621 gctgttatgg gtgggcggca ggatggtcat tggcagtact aaacaggaag aggagtgccg
    34681 gaaaaccgac cccaccaggg gcggctcgct ggtagccctc tccgttgcca ccagtataga
    34741 cgcgctggca gtgggcttga gcctggcgat tgtggatggc tctattttca tcagcagttc
    34801 tatcatcggg ctgacttcat ttgccctttc tgtgtctggc tacctgttgg gcgacaaact
    34861 gggttgccga tttggtaaac ggatggaatt gttgggtggc ttgctactga tcggaattgg
    34921 catcagggtg ctgttgagcc atctgttggg gacctaaata attcttgacc cgactcagtg
    34981 atgggactat aatgtttcac attattgcga caagcaggaa acagaggtga tactcatgcc
    35041 ggtatacaca tatcggtgcg aaaactgcgg tattcagttt gatcagactc aaaaattttc
    35101 tgacccggcg ctaacccgct gccccgagtg tggaaagaag agcctgaaaa aggtctatac
    35161 cccggtgggg attgtgttca aagggtcagg cttttacgcc accgaccacc gttcaccgtc
    35221 aggcgctgca cataattcca acacgaaaca cgaagaagcc aagagcactg aaaagacgga
    35281 aacgaaaact gagaccaaag cagccagtca aacaacggtc gaatccacca gcaccccgag
    35341 cggcgataaa gcctgaaacc ctccatttga agatcaacct accaggaaga atcataccgg
    35401 gttacgggtt gttacccatt gaaagggatg tgataggatt tgttaaaccg ttgatagaaa
    35461 atgatagttt gcggaaaata aactcccgag catatagcga ggtatatcat ggcaaaaccc
    35521 aataaacaaa caggcgcgca aaagcggtca aaaataacat ccagccagcg caagatgcgc
    35581 gcgcaacaga taatcctcgc ggtgatcggt atcgtggtga tcatttccat gatactggca
    35641 ctaaccatga aattctagaa gaaatatgcc taaaaaacaa aagtggtttt ggttggcagc
    35701 gctgattgct gcctggttgt ttgatttttt gttctggagc aaaggctcag gtgtatcctt
    35761 cttcatctgg atcgccatcc tgctgctggc gggttacacg ctggcgtgga gcgaggggaa
    35821 gaagccgagc gcattgagtg tgctgcttac atgtttcatt ctcggttttg ccgcggtcat
    35881 agccttgcgc aacgaaccgc tcacacgggc ggtgagcata ttgctgacgt tgaacgggat
    35941 gctgctgctg acggcaacgt tcttgaacgg gtactggccg tggtacagga taaaggatta
    36001 tgtagtggaa ctggtccggg tgatcggagc gggattttct ggcgcgatct ggctgttggc
    36061 aaaaggaaaa accccgccgc ccgtggatgg ctctccagcg aaaagcggat ggagcaaagc
    36121 ctggccggtc attcgcggcc tgctcattgc gctccctgtt gtggctgttt tggggttgct
    36181 gctggcttcg gctgaccagg tcttctctga ctgggtcaag cgtattttga atattgaaaa
    36241 actgccggaa tacctgttca gactgtttta catcctgatc attgctggtt ttctggtggg
    36301 cgtgtacctg aaggcgatat accccaggaa gacggcccaa aagcccgaac caaaccaggc
    36361 gtggcttcaa cctttcctcg gttggactga aagcgggatt gtgctggggg cggtgaacct
    36421 gttgttcatc attttcgtca ttatccaggt gcgctatctc ttcggtggtg aagccaatat
    36481 cagtgaaact ggctttactt acgctgagta cgccaggcgc ggcttcagcg agctggtagc
    36541 ggtggcggtg ttgagcctgg gtttgtacct gctgctgggt acggtcacaa aaaccgagag
    36601 ccaggggtcc aaggtcgggt tcacagcgct ggcagtgttg ttgatggtga acgtgctggt
    36661 gatcctggct tcctctttcc tgcggttaat gttgtatgag aacgcttatg gcttttcacg
    36721 ccctcgaact tacacccaca ttttcattta ctggctggcg gggttgatcc tggcggtgat
    36781 ggtgctggag ctgatgaagc gtcgaggacg catcgcgctg gcgctgttgg tggcggtggt
    36841 ggggtttggc gcgacgatgg ccatcctgaa catcgatggt tttatcgttc agcgcaacgt
    36901 ggctcgagcc gaagctggtg ctgaactgga tgttgtccat ctgaccacct tatccgctga
    36961 tgcggtgccg cagctcctcc aaaagtatca gtcgcctgag ttaagaaaag aagtgagaga
    37021 aggcttgggc gcaacgctgg cgtgtatgga aaaggccatc caagacccgg cagatctccc
    37081 ctggcaggaa tacaactttg gccaggcgcg ggctgcgcag ttgttacggg aaaacgcgga
    37141 cagctggagc caatataagg cttaccagac ggaccgggac tgggtggttc gcctgaatgg
    37201 gcagaggatt tactgcgcct atttcatcat ggattaactg gcataactga ataagcagcc
    37261 cactctgagc gctcaggcgg gctgcttttt tctatgggaa tgttaaatga ggtcaagttc
    37321 gtttttcacc ggggagaaga gcacgccatc ccagccgaaa acagacagat cgatggaacc
    37381 ctggtcatca aaaaggatac cctcttcttc caacaggaca cgctgccttt ggctgcccat
    37441 accaaagccg cgtgggctga ttttcccctg cgcgttgata atacgctgcc agggcacttc
    37501 ttcagcaata tcacgcccac ggtagacggg cagcgctgcc agcgcaaacc ccaccatctg
    37561 cgcggagcag cggccggcga tgcgggcgat ctgcccgtaa gtggccaccc gtccgcgcgg
    37621 cacctggcgc acaatggcgt agatgagttc atataagcga ggctcttctt ccatgtcact
    37681 gttattttac cgcttcaggc ttcaagcagc gcgtatagcc ttcatggtag aattcgaagc
    37741 atgaaaagat ggcagttctg ggtgggtctg ttaatcagtg ttgcatttct atattttgcc
    37801 gttcgcgggc tcaacctggc cgatttttgg aaagcggtga aagatgccaa ttactggtgg
    37861 atacttccgg gagtggcggt cttttttgct gggctatggg tgcgggcgtg gcgctggcat
    37921 tacctgctgg ggccggtgaa gaagataccc acccgcatca tgttccccat cacctgtatc
    37981 ggctacatgg gtaacaacat ttacccggcg cgcgcgggag aggtattgag ggcggtagtg
    38041 cttaaacgcc gtgagaaggt gcctatttcg gcttcgctgg ccaccatcat cgttgagcgc
    38101 atttttgatg gcgtggtgat gctggcgttt gtgtttctca acctgccggc gctggcaaaa
    38161 gtctcctctt ttgagtctgg ctttgttggc aatattcaga atctggcgct catcggtacg
    38221 gccatttttt tggcggcgtt gctggtgttc ttgctggcag cgatgttccc cagaacaacc
    38281 gccaggctgg gtggatggtt cattgagcgc ctgctgccac cgcgcgcgcg tgaaaaaact
    38341 accggcatca tgctcaagtt cctggatggg ctggaatctt taaggtcgcc attcaacatt
    38401 ttgatggtct tttttacttc ggtcatcatc tggttgctgg agacggtgaa atactggttt
    38461 gtgatgcagg cgtttacttt ttcagtgagc ttttttgcct tgatgctgct gaatgggatc
    38521 gcgaacctgg ccactaccat tccttcggcg ccggggtata tcggcacctg ggaagcggtg
    38581 acccgggcgg tgctggtggc atttggcgtg cccggggctg aagccctggg ttatggcgtg
    38641 gtactgcacg tggcgctgtg gctgccagtt acgctggtcg gcgcgtacct gatgaccaga
    38701 gagggtatca ggtggagcga ttcgctgcga caggaggccc gttcaggcga agctgaagca
    38761 ggcggggaaa tttgcgataa ggagagcaat aaatgaaaat cgcggtgatt ggcgccggtt
    38821 ttggcgggtt ggcagctgcc tatgacctgg taaaagcggg aaacacagtt acgctttacg
    38881 aagcggagag cacccccggc gggctggccg caggcttcaa agaacctgac tgggagtggt
    38941 cagtggagaa atactaccac cactggttta cctccgatga acatatgttc cgcctgttca
    39001 gagaactggg cgttgaagac agggtcatga tcttaagccc aaaaacggta atgtatcaca
    39061 aaggcaagtt ttaccccttt gattccatca ccagcgcatt actctaccca gggctgggtt
    39121 acggattgaa caagatccgc tttgggttgg tggggctgta tctgcgcttc accaaccgct
    39181 ggcagcccat ggaacaaacc accgtagccg agtggatgaa gaaatgggct ggcgagaagg
    39241 tgtacactca gatgtgggaa ccgctggtga tgggtaaatt cagcgaaaaa tacgccaacc
    39301 aggtgaacat ggcgtggatg tgggcacggc tgcacgcgcg taccacccgg ctggctactt
    39361 accagggtgg ttttcaagca tttgcggatg agtttgccgc gatcctggca aaaatgggcg
    39421 tcaagttcgc ctaccgcacg gtggtgaaac aattgatgag cgaaaaagaa ggtggtctga
    39481 cgctctccac cactcatgga aacgaaacct atgaccgggc gctggtgaca atatcaccgg
    39541 cgctgctgtc aaaaatggcg ccacagcttc ccgaggatta cctcaaaggg ctgcttgatc
    39601 tgaagagcat gggcgcggtg gtaatgaccc tcgcgctcaa acatcaactt tctcgcgaag
    39661 ggtactactg gtacaacctg cccaaagacg cagggtttcc attcctggcg ctggtggaac
    39721 ataccaactt tctctccccg caaaaattcg gcggcaacca cattatctac tgcggtgatt
    39781 atttagaccc ggctcacgag tattttcagc tttccaaaga ggagttatta caacgtttta
    39841 cccccagcct aaaaaagatt aacccgaact tcgaaacggg atggatcacg ggctcctggc
    39901 tggtgaaaac caactacgcc caaccggtgc cgctgctgaa ccattcccgt catatccctt
    39961 caatcgagac gccccttaaa gggctgtatt ttgccagtat gtcgcaggtg tatccgtggg
    40021 atcgcgggac aaacttcgcg gttgaaatcg ggcgccgggc tgccgacctg atgctgcggg
    40081 cgtagctgat tgcgcgctga tgaaccaggc aagcgccata cttaaagacc agatttgaac
    40141 ctgaaaatta aaaattcctg atgccaaaaa atgcattatg ggtgggtttg gcacgaggaa
    40201 atggaaacct gtaactttta taataaggcg ccatatagcc gcagcgaggg taaaaaccgc
    40261 ccatcatccc cagatgtagg ggcttcggtt gcgttgatac cgcagggata aggatataaa
    40321 taatcggtaa cgattgcgta atccatcaaa gtccccaggt tttcttaagg ttgggaacct
    40381 taaagatttg aattctttta agttagcgta gggtattcga cttgttaccg gggcggcttt
    40441 ctgttagaat gcttatacgc gttcggacga agcttaatcc acagcttcgg gtaagccttt
    40501 gtaggatcca ctagaatatc ctgttcccaa tccctgctta cgctggagtg gcgggttcta
    40561 ctgacctacc ccgtccgtga cgtgaattcc aggcggaggc gcgtgttgga tcatcggaac
    40621 gcatggcagg cagcggttgg acaactccaa atggagatgt cgcgcgcatc ttatgaaacc
    40681 tgggtacagc ctgccgaact ggtgcgttac gaaaatgacc tcttcacggt tggcgtgccc
    40741 aacgcttacg cctgtgactg gctcaaatcc aggttgaccg caaaactcaa cagtattctc
    40801 agcggcatca tggaatgccc tcaaaaggtc gagttcatcg tctggcataa ggattacgaa
    40861 aaccagccgc agacagccag taccgcggaa cctttaccgt tgatctctcc cgccgtaaca
    40921 ggctctcaac cggtggtgaa ccatcgttat aacttcgata acttcgtggt gggttcttcc
    40981 aaccggctgg cgcacgcggc ctgcctggct gtggcggaga accctgccca ggcttataac
    41041 ccgctgttcc tttatggcgg tgtggggttg ggcaaaactc acctgctgca cgccattggc
    41101 aactttgccc aggcgcgcgg agagcaggtg ttatacgttt catctgaaga attcaccaat
    41161 gacctgatta acgcaatccg cacacacaca acgcaggctt tccgtgaccg ctatcgtcgt
    41221 atcgatgtgc tgctggtgga tgacatccaa ttcatcgccg gcaaagagtc gacgcaggaa
    41281 gagttctttc ataccttcaa taccctgcac gggcaggata agcagattgt catctcttcc
    41341 gaccgtccgc ccaaggccat gaacacgctg gaagaacgct tgcgctcacg ctttgagtgg
    41401 ggattaacgg cagatatcca gccacctgac ctggagacac gcatcgccat cctgcgctcc
    41461 aaggcggagc ggtcaaggca cgcggttccc ccagccatcc tggaaatcat cgcgcgccag
    41521 ttccaatcca atatccgtga actggagggc gccctgaccc gcgtggtggc ctacgcggat
    41581 ctgcgcggac gcccgttgag cgaagaactg gtcaacgtgg cgctaaccga tctcttgccg
    41641 caacggaatg cttttgaacc ggcacacgtg gtgaacgtgg tggcatcggc atttggcatt
    41701 acccctgaaa aactgatggg gcgcgaccgc acacgcgcag ccgctctacc tcgtcaggtg
    41761 gttatgtacc tgctgcgcca ggaagccaac gtgtcgctgc cgcagatcgg cgaagccatg
    41821 ggcgggcgcg accacacaac cgtgatgtac gcctgcgaaa aggtggcgga tatgatcgaa
    41881 agagatgacc gtctgcgccg ccaggtgctg caaatacgcg aacagcttta cggaagaaaa
    41941 acaacctgac cgttgtggtg gtcaggttgt tttttttttc caggtatatc gaaattagga
    42001 tgctggctct tcgaaaagcg ctcgcttaat tttgggcagc agcaaaatga ccagcagcgc
    42061 agtgctcaac agcgcgccga tgaggtaatc gatcgaagta gcatcagtgc gcagcgtgcg
    42121 gataatttgc gtgggggtgt tcatcgccac cagtgagacc gccgctacca acgccatcca
    42181 ccagccttcc attttgcgcg cggcaatctt gtaaatggca acgaacagca ggatcacgca
    42241 aataaattgc accgggctgg accacgccag cacccagccc tcgatcccgc tgtaaagcgg
    42301 cttttcgagg cgggtgagcg tttggcgcat gccccccacc gcgatgacga aggcgtgggc
    42361 tgtcataatg ccgcagaagg tgagcgtgag cagctgggcg atcttcttct gcgtgtttac
    42421 ttttctgagc aggataaggc agaggaagaa caacaggccg gtaagtgata caagcatcgg
    42481 caggggcagc ccaggcacaa aactgacata aggcagcatc atgaacattc cgcctgaagc
    42541 aggaaaagcg gacgccagca gacctaccgg aaaagtccat tccttgcctt cgcggatgga
    42601 gcgggcaatc acaatgagcg taatgccggc gatgaagatg atcgcacggt acagcgggta
    42661 gagcactgaa aacaacagga tgccgctggc ataagccggc ttttgggcgg aaactttcac
    42721 cagggcggtc atcacccggt ctaatgaggt ttgaataaga aacggagcca cccccaccat
    42781 gaacaccccc accaccaccg ccaggatggc caaaaacaaa cgggttttac tggaagtttg
    42841 agacatattc cctccgaaaa aggttgatta ttcttatgta tattacctga aggagaaaaa
    42901 aataccttgc gaaaagttaa acttggtgga aaggaaacct gaataatcgg ataaaataag
    42961 gtataataaa cgggtaatta agggttcatt atcaacaagc aggctagttc tgga
//
LOCUS       DAOSPM010000004        35247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1246_length_35247_cov_15.349937, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000004 DAOSPM010000000
VERSION     DAOSPM010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 35247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35247
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1246_length_35247_cov_15.349937"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PLW25_00995"
     CDS             <1..1128
                     /locus_tag="PLW25_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOS53094.1"
                     /translation="FIHFYFAGVIIMSVDFIFRILGMVILAIVGGILGNQFGEIANTS
                     PSATTFPVEQYRFLFGLLGALFGLILTPYFTTRPARAMRNALAKATTHALVASLFGLV
                     AGLIVAALLAFPLSLLPDPFGSVLPFVGAILFTYLGVVIFKLRQQDFLNLLSNLRSGS
                     GVTAKDGASGAGWAESRTILLDTSVLIDGRIADISRTGFLPGSLLIPRFVLNELQYIA
                     DSPDNMRRQRGRRGMEVLSALQKEPAIPVRISDIDVEGMREVDDKLVVLARQMRCPIL
                     TNDYNLNRIAELQGVTVLNINELANAVKSVLIPGEIMTVQVIQEGRETGQGVAYMDDG
                     TMVVVENGREYMDKSIQVTVTKVLQTAAGRMIFARPDEAAN"
     gene            1148..2542
                     /gene="cysS"
                     /locus_tag="PLW25_01000"
     CDS             1148..2542
                     /gene="cysS"
                     /locus_tag="PLW25_01000"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOS53095.1"
                     /translation="MKVYNTLSRKVEEFQTLEPGKVKMYVCGPTVYAKAHIGHAMSAL
                     VFDIIRRYLEYRGYEVRHVMNFTDVDDKIILRAQEKGVDPFKLGEMYIHEFEKHLQDF
                     NILPATVNPRATREMEQIITMIQGLVEKGFAYDKDGDVYFRVTSDSQYGKLSGRRIDD
                     MQSGYRIEVDERKEHPMDFALWKAAKPGEPAWDSPWGKGRPGWHIECSAMNLNHLGEQ
                     IDIHGGGNDLVFPHHENEIAQSESYTGKTFARYWIHNGMLQLSGEKMSKSVGNLVTIE
                     EFLENHPADALRMLVLNSGYRNPLSFSDEILAQAEKAIERLRSGLKPAPAAAPGAAQS
                     VVDALARQVETAESGFVQSMDDDFNSAGALAQLFDLVRGINQARADGATHAQLSAAQL
                     KLRQLTAVLGLSLEKETETHQEVDGFVNLLIEIRRELRANKLWELSDSVRDRLAALGV
                     VLEDSKEGTSWHWK"
     gene            2550..3287
                     /gene="rlmB"
                     /locus_tag="PLW25_01005"
     CDS             2550..3287
                     /gene="rlmB"
                     /locus_tag="PLW25_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOS53096.1"
                     /translation="MKEWITGRNPVMEVMQAKRRQVFRLLLASGVEEKGRIAEILRAA
                     ASIKAPVERVARERLERLGENPQGVAAEVGVYPYVELYDIYDLAKERNEALFVLVLDV
                     IQNPQNLGTLLRTAEAVGVHGVIIPQHRAAEVTPAVVSASAGASEHLLITRSNIARSL
                     EQLKEAGAWVVGLAEGEDAPAEVPLGGPIALVVGNEGEGMRALVRQQCDYILSLPMRG
                     RIQSLNAAVAGSIVLYQVLLARQKTSR"
     gene            complement(3576..4076)
                     /locus_tag="PLW25_01010"
     CDS             complement(3576..4076)
                     /locus_tag="PLW25_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOS53097.1"
                     /translation="MDAIANSNWFVPVLAVVMGVGLFGLMLYIIPGLTIIWLAILAYG
                     IVSGFSPATGILFAVITLLMIGGNLIDNLFMGAEAKKTGASWISVVVALAAGIAGTFI
                     LPPFGGLLFAALSILVVEWVRKRDLKEGAKSAGAIIRGCGFATIARLGIGVLMIGLWV
                     VWVLWL"
     gene            complement(4079..5347)
                     /gene="serS"
                     /locus_tag="PLW25_01015"
     CDS             complement(4079..5347)
                     /gene="serS"
                     /locus_tag="PLW25_01015"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOS53098.1"
                     /translation="MLDINFIREHPEELRKALTDRQMDSSVVDKLLTMDAERRGLLVK
                     VENLKALRNTVSKEISQTKDPAERQEKIDAMRSVGEEIAQLDETTRQVDEGLQALLAT
                     IPNLPDPSTPIGKDESENIVLRTHGALPKFDFTPLPHWELGPKMGLIDFDRGVKLTGS
                     RFYVLNGEAARLQRALISWMLDLHARQGYVERYTPFMVKAETLYAAGQLPKFVDNLYH
                     DIEEDLWMVPTAEVPLTGMYRDEVLEEASLPLRFTAYTPCFRREKMSAGRDVRGIKRG
                     HQFDKVEMYIYSTPEESMHELQKMLADAEETCALLGLPYRVKQLCTGDLGFNSVVTYD
                     LEVWAAGCNEWLEVSSVSNVGDFQARRANIKYKPAEGGRNRLVHTLNGSGLGLPRTLI
                     AVFENNQQADGKIKVPEVLLPWMGGLKVIS"
     gene            5743..6354
                     /locus_tag="PLW25_01020"
     CDS             5743..6354
                     /locus_tag="PLW25_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS53099.1"
                     /translation="MDEQALVADALQGNVDAFNRLVLAYQDMAFNLAFRMLNDDDLAA
                     DAVQNAFLSAYRNLRAYRGGSFKAWVMRMVTNGCYDELRRQKRRPTIPLEPQNPEDGE
                     EIESADWLASDDPQPEQAFDQAELEHALQHCLENLPPDFRAVVVLTDVQGMDYEEVSR
                     AVRTPLGTVKSRLARARQRLRDCLRQFQELLPTQFRLTGGERR"
     gene            6351..7208
                     /locus_tag="PLW25_01025"
     CDS             6351..7208
                     /locus_tag="PLW25_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53100.1"
                     /translation="MTERLTSRDWILISAYLDGRLSPAALTRIEKRIQEDAHFKRSLE
                     EIARLKQLLAALPKKRAPRNFTLAPEKAQKPLRGLWLQPALSFVSVTASILLVLVFSF
                     NYLGMGLPRQAAAQRAEIAAMEGVAEAPAPMIINWIPSGGQGGGAAEAKSPYTGIDGI
                     GGPGFPVAEPGIGGGPVEPAVPELPPGVDPQPVMNEEPEPPAYDEPAPAALEGEPVSD
                     ADLSNLILGLAEDDTAGQVIESAPLRKAPAPQPRRGFSAPSLVMLIAGITALLSGAGA
                     LFLRKKFHA"
     gene            7201..7713
                     /locus_tag="PLW25_01030"
     CDS             7201..7713
                     /locus_tag="PLW25_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOS53101.1"
                     /translation="MHSHSHEHFKFCPQCATPLRMMMQLGERRPSCPACGWVHYEDPK
                     VAAGVLLLRGREVLLVRRVMEPYLGTWSIPAGFVNAFEDPAAAALRECREETGLTVEI
                     EGLFDLLTGREHSRGADIFIVYRAHFLSGSLAAADDADQVGWFPLFDLPPLAFETTRR
                     VLEKARHLQL"
     assembly_gap    8082..8181
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8182..10451
                     /locus_tag="PLW25_01035"
     CDS             <8182..10451
                     /locus_tag="PLW25_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOS53102.1"
                     /translation="YSRSTLTLDQRLNFIGGVTVLTGVLSIVALFSNANGPLSGWIAQ
                     TTTRLAGWGGVIIPLALIIGGTVLLLRHYENLPRVSYWRLAGIILLYLNILGWFHFIA
                     GGGFSTAKDGRGGGYIGALFDRVLANSLGPAGEGVFLFAWLLVAILFILNLPLVEIAA
                     RVNTLLVRFKKPKEENPRQPMFHFTSARPYRVKNESRTDLPDGYKPLVVEEESPATVT
                     VIGSPSAAEPKTTGVREKPARVAPEPSESLPVTRTRVTTPVAPWPIPAVSDILDLATP
                     AVVQSHYDQGRARIIEETLASFDAPGRIVEIQRGPAVTQYGVEPEFIESRGGRTRVRV
                     SKIAGLANDLALALSAPSIRIQAPVPGKAYIGIEVPNSELSLVSLRELIETDAYKKIR
                     SSLRFAFGKDVAGKPICADLTSMPHLLIAGTTNSGKSVLVNAILTSLLLNNSPLDLRL
                     VLVDPKRVELTGYNGIPHLLAPVVVDAERVAGALQWVLGEMDSRYRRFAEEGVRNIMD
                     HNRKEAERLPYIVVVIDELADLMLLAKDETERSITRLAQLARATGIHLIISTQRPSVD
                     VITGVIKANLPARVAFMVASNLDSRVIIDKPGAETLLGRGDMLYQAPDAPAPVRLQGV
                     YVSDQEIQRLVNAWRLIVANRRAEGGKELFTATFEYQPQHVPFKQPSFFGDGAQEDDP
                     VLKNAIEIVRKEGKASTSFLQRRLGIGYTRAARLIDTLEEQKIIGPRQMGSQVREVLD
                     FGADNEEPPAETQSD"
     gene            10644..11603
                     /locus_tag="PLW25_01040"
     CDS             10644..11603
                     /locus_tag="PLW25_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53103.1"
                     /translation="MRKRNSFFLLIAVLLLGFLASCRSARLPLPAFLSADTPTPTATM
                     TFTPTYTPLPTKTFTPTPTPPVNLIPCANSDACSEGVYIREYLETVGLLDEADEGERP
                     FEVEVPYNLPLRFGLSWIAIDDETLADNMKKMTFFFEIDGQPYLDKNDLIYEYYPDVE
                     DPSIVYSSVFIDYLLTGWRIGEPHMVSIGFRFDQSLSDGWNTYYLGDTIGYSYRILPV
                     ELPTATPTPSPTSTPQPRPTAVPPTPTVACELGTTIEIKNDTGGQVTMHFTGPVKYTF
                     YIAPGSRTLQLCTGQYSYTAYGCGGSSTSGSAGDGDEIEFWCE"
     gene            complement(11651..12061)
                     /locus_tag="PLW25_01045"
     CDS             complement(11651..12061)
                     /locus_tag="PLW25_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53104.1"
                     /translation="MNNHPLQPPNVQAPRSSLLKLVIISAFIAMVAFCAPAGVLAVIF
                     TRPLFALTTGVVCPPGSEMQFEEWEEEGDWEEEASTQFRAYCVDSSGQQVQERTLQAL
                     AVLMCSFFLAGFFIVLAVLLIVRAVRHRNRSTNP"
     gene            complement(12266..13480)
                     /gene="metK"
                     /locus_tag="PLW25_01050"
     CDS             complement(12266..13480)
                     /gene="metK"
                     /locus_tag="PLW25_01050"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOS53105.1"
                     /translation="MSGSSFMQNPSFKFTSESVTEGHPDKLCDQVSDAVLDACLEQDP
                     RSRVACETAVKTGYVMILGEITTNAFVNFNDLVRQVVNSIGYDSSDKGFDGNTCGVLS
                     AISSQSQDIALGVNKSYEAKGGEDKETELEHIGAGDQGMMFGYACDETPTLMPMPIYL
                     AHRLTQRLAEVRKAGLLPWLRPDGKSQVTVEYAYGKPVRVDTVVVSTQHTADVSHADI
                     TEGVIEHVINPAIPAELLDGTPKIYVNPTGRFVIGGPMGDAGVTGRKIIVDSYGGMGR
                     HGGGAFSGKDPTKVDRSAAYAARWAAKNIVAAGLATRCEIQLAYAIGVARPLSINVET
                     FGTGVIPDEKIAALVSEHFDLRPGAIIRDLDLRRPIYRKTSNYGHFGRELPEFTWERT
                     DKAGLLRSAAGL"
     regulatory      complement(13556..13664)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(13783..14745)
                     /locus_tag="PLW25_01055"
     CDS             complement(13783..14745)
                     /locus_tag="PLW25_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53106.1"
                     /translation="MSSSSSSSAKTAHDLYTCPHLGMLDDTTTTTAFPSEVNHCLHCQ
                     SVCVPSRQQQQEYCLSQRYRDCPILHQAAPARLPASLRWVQNPAVFRAIFIKSALAVF
                     GAGLLAGFFLLGAPARISHIIQAYVPEDTQTPYQPYDFPTRTPSPRPEQPSPTATSLL
                     SHLEIPTALPISQIVQLTVNEVELGVNCRSGPDSSFESYLILRTGDVLQALARSPDDA
                     FFYVRLPKKRAPDCWVPAAFVNVLGDTHTLPVLTPAPTPHPTVTLTPLPSTPTETPQT
                     PPPTTLPTSTTQVNTPPPSQPTAESPEPTEEPPEPTEEPSATPG"
     gene            complement(14971..15891)
                     /locus_tag="PLW25_01060"
     CDS             complement(14971..15891)
                     /locus_tag="PLW25_01060"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOS53107.1"
                     /translation="MFEIIFLGTSASAPSPRRGLPAYLVQHDEYRFLIDCGEGTQRQI
                     LQAGIGFKRLNHILITHGHLDHILGLGGLLSTFMRWETIDRLEIYGGANALERISDLV
                     HGVVLRGARAPMPVELIPVTPGVIFEAPSFTISAFAVNHRGPDNYGYLFEEKGRQPFL
                     AEAADALGVPAGPLRGKLVKGESVTLLDGRVITPSMVTGPFKPGARLAALGDLGETAS
                     LTEVLRSVDGLVMEATYLEEEASMARQYAHLTARQAGEFAASIGAGKLILTHLSRRYR
                     DKDILAEAQAVFENTSVARDFDSYTIRKEA"
     gene            16048..16536
                     /locus_tag="PLW25_01065"
     CDS             16048..16536
                     /locus_tag="PLW25_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOS53108.1"
                     /translation="MEFLSVSIHKPEALNFILGQSHFIKTVEDIHEALVTAVPGIQFG
                     LAFCEASGACLIRSSGTNEALTQLARENAMNIGAGHCFILMLGEGYFPINVLNLVKNI
                     PEVCAIFCATANPTEVVIYDNGYGRGIMGIVEGEKPKGFESPEDVRWRVDFLRRIGYK
                     AG"
     gene            16572..17519
                     /locus_tag="PLW25_01070"
     CDS             16572..17519
                     /locus_tag="PLW25_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53109.1"
                     /translation="MKKLIPVLIILSLVLTACGNLPLGRTEEDMSDSEMATRVAELLS
                     TMTTPTTEMVFPPTPTPQAATEAPPEPSAAPATQEPAALPATETPVVVEETLLPEEAG
                     TPTTEVTPALPETDPARKLGNPTHSDPLDSYEKWTWPTGSDDYLKVNFKDGFLEMTGL
                     TTMAGWRLPILAQQTNSYIELTVNSGACQGKDSYGIIFRVPVFKEPTQGYLYEVTCDG
                     YLRLWEWNGKAKPKGEADVLINWKESMDIKTGANQVNRLGVMAVGKSLRIYMNGIPQG
                     EVSDSTYPAGFVGAFVRSVTTPKYTVKFDEMKVWENPAQ"
     gene            17649..18068
                     /locus_tag="PLW25_01075"
     CDS             17649..18068
                     /locus_tag="PLW25_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOS53110.1"
                     /translation="MKSYRKEITLNIPARRGFVNITPQVQACLRESGISEGLVLVNPM
                     HITASVFINDDESGLHRDYDAWLERLAPHEPVNQYRHNDTGEDNADAHMKRQLMGREV
                     VVAVTGGRLDFGTWEQIFYGEFDGRRPKRVLVKIIGE"
     gene            18070..18927
                     /locus_tag="PLW25_01080"
     CDS             18070..18927
                     /locus_tag="PLW25_01080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOS53111.1"
                     /translation="MSNRVYFGENLEVLRALPDASVELIYIDPPFNTGKVQARTRLRT
                     TRAENGDRVGFQGQRYQTVRLGSLGYNDIFDDFLAFIEPRLLEAHRVLSAAGSLYFHI
                     DYREAHYCKVLLDSIFGRACFLNEIIWAYDYGARTRRKWPPKHDTIFWYVKDPLRYTF
                     NYDAIERIPYMAPGLVGPEKAARGKIPTDTWWHTIVPTNSGERTGYPTQKPLGILRRI
                     VAASSNPGETVLDFFAGSGTTGAACLELGRRFVLVDNNPQALAVMTERFKGVTDIEWI
                     IDTPEAMDR"
     gene            complement(19104..20024)
                     /gene="lipA"
                     /locus_tag="PLW25_01085"
     CDS             complement(19104..20024)
                     /gene="lipA"
                     /locus_tag="PLW25_01085"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOS53112.1"
                     /translation="MANTPTRDRIQPAPSDPRPRRRPDWIKVKAPTGETYAQLQALMR
                     RTSLHTVCEEAGCPNMGECWGSGTATFLMLGEICTRSCRFCDVKHGRPAPLDWEEPER
                     VAQAVKAMNLKHAVITSVNRDEREDGGAPIFAAVIRRIHELVPGCSVEVLIPDFKASL
                     PALKMVMDARPEILNHNVETVPSLFKKVQPQDHYEWSEAVLRHAKLLAPDVLTKSGLM
                     VGLGETMSELKAVMRDLRDWGVDILTLGQYLQPSRHHLPIERYYTLEEFADLKEYGLS
                     LGFGWVESGPLVRSSYHAAEQVRALNPTFR"
     gene            20148..20816
                     /gene="rsmI"
                     /locus_tag="PLW25_01090"
     CDS             20148..20816
                     /gene="rsmI"
                     /locus_tag="PLW25_01090"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOS53113.1"
                     /translation="MLILVATPIGNLGDISQRALAALENADVVVSEDTRKTGFLLNHF
                     GIQKPQISFREDNEQRALPKVMEFLELGKTVALVTDAGTPSISDPGFILVRSAIEKAI
                     EVSAVPGPTALIMALVLSGLPVHSFTFRGFPPRKSAARQRWLEIDCDSPHTLIFYESP
                     HRLLAFLQDAQAVYGNRRCAVANDLTKKFETVYRGTLKEVSEQLAREKLLGEYVVVIA
                     GAED"
     gene            complement(20813..21538)
                     /locus_tag="PLW25_01095"
     CDS             complement(20813..21538)
                     /locus_tag="PLW25_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOS53114.1"
                     /translation="MDKTPQRSYRYFDLVMALFVTVLIISNIASSAKIVDWGVSIFNL
                     PLAFDAGTILFPISYIFGDVLTEVYGFKRSRRVIWTGFGCLALTAAVFWLVRVMPGEA
                     TWVEQSGQGAYNAILGGLTSGGIVLASLLAYLAGEFMNSIVLAKMKVATRGKWLWART
                     IGSTLVGEAVDSLVFVSIATLTGVFPWSLFWTLTVTNYLFKTAIEVLMTPVTYWVVST
                     LKRQEQEDYFDTHTRFSIFDWNG"
     gene            21611..22384
                     /locus_tag="PLW25_01100"
     CDS             21611..22384
                     /locus_tag="PLW25_01100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOS53115.1"
                     /translation="MQRFFVNPEEIQGQEVVFAPARSRQIARVLRLKNGQNCMVLDNR
                     GGELLVELTQVEAGACRAVIVERAEAQQERIDLLMLLSLTQREKFEWMLQKCTEVGVS
                     TFQPVITSRSLVQQPVQTIAKYERWKTIIWEAAEQSARSRLPELLPPAGLAEAVAEAA
                     RRCQRCLIPWEGEKARTLKGALAGPAVGSVALFIGPEGGFAEGEVQQAVNAGFNVVTL
                     GRRILRMETAAVVSAALVLHELESTEADDGGTGSSGPIG"
     gene            complement(22335..23831)
                     /locus_tag="PLW25_01105"
     CDS             complement(22335..23831)
                     /locus_tag="PLW25_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53116.1"
                     /translation="MSQKPNRRQRPTVPPADSGEMAAAYKPLLTEEEFKLLVNEINQP
                     LPTAIRLNPLKAPPNLASRLSEKYGWQLEAVPFCPAGYRVTIGAGPEVSAVLEHRLGL
                     MYIQEAASMLPPELFNFSPQREELVLDLAASPGGKTTHLISRLEDSGLVIANDSSQGR
                     IQALRLVLQQWGAVNAAVSRFPGERFGEWFPSMFDKVLLDAPCSMQGLRTAESHISRP
                     VTEKESRQLSRRQLALLTSALRTLRVGGELVYSTCTLLPQEDEMVVEELLRVFGSAVE
                     ILDAQTKLPAPAPAITSLDGAELHPALSKAIRLWPHRYHTAGFFACHLRKIEHTPMRP
                     TSPPSRPLAQVGFEELTAREQRAFCEEFHNTYAYDLLADISSHQRTLVRHFEEMHLLP
                     MLLLEKFPALPLQSAGMLLGQQTASGFLPSHQWASRFGARCQRNIVQLDEIESRLWLS
                     GADLLPRGSPAKETDRFRIILNQEGLSLGCGKVTQSALKNLFPRHLLL"
     assembly_gap    23950..24049
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24050..24816)
                     /locus_tag="PLW25_01110"
     CDS             complement(<24050..24816)
                     /locus_tag="PLW25_01110"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53117.1"
                     /translation="MKKISASPTEDQYAALLARVSASRKYRALGIPDTTLRDLLEKTL
                     RACSDPRQVEKVFRQKLHNLVATYLGDPDYTMMRQALAALPNQPHAAEVMEWCAQLLK
                     AHASTRERLPMLSLFYQEIFRFTGTPRVVLDLACGLNPFALPWMGLPRETAYYAYDLH
                     QPRVDLIDAFFQHIGQTGSAYHRDILVEPPEMEADVVFFFKEAHRFEQRERGVNRQFF
                     HRLRTRWLLVSLPTVSLNGQRSLLTSGRRLVAEACAQR"
     gene            complement(24830..25642)
                     /locus_tag="PLW25_01115"
     CDS             complement(24830..25642)
                     /locus_tag="PLW25_01115"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53118.1"
                     /translation="MAEPDTLLDALVEQVQRAEKYRHLSEVLVRSVLTAEMEKRRGPK
                     EAVKAARSKLHQVAAAYQGKPIPYAAWLKELAQLPQDLHHPDVLSYLMHALPAHASTR
                     ERIPIQAVFFQECLAPLGSVHSILDLACGLNPLVIPWLPLQPGFTYTACDIYTDMLDF
                     LNAFFARFKIDGHAVPCDITRELPPVKADLTLVLKTIPCLEQIDKDAGRRLLEMISSP
                     NILVSFPAHSLGGKSKGMVRNYERRFAELLAGKAWQVTRFEFPGELAFLIQR"
     gene            complement(25635..26741)
                     /locus_tag="PLW25_01120"
     CDS             complement(25635..26741)
                     /locus_tag="PLW25_01120"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOS53119.1"
                     /translation="MRAANLELVSGKVHFPVFLPDATYGVVRTVDARDLAGIGIEALV
                     MNTFHLTQKPGSSTIQALGGLHQMTGWHRPIITDSGGFQAYSLIRQNARYGSINDKGI
                     LFYPEGSSRKFQLTPQKAIQLQMSYGADIVFCLDDCTHVDEDEAAQRLSVERTIAWAR
                     LSKAAYLRLAEQKEQTGQQRPLLFAVVQGGGFKEQRKRCADALLEIGFDGFGFGGWPL
                     DGENRLLTEIIAYTRQLIPAAFPMHALGIGHPQNLLATYHLGYDLFDSAMPTRDARHG
                     RLYTFTSAPSPGAPGLTGDWFAYQYVDNPRSIRSSQPISQWCDCPCCRDYPLGYLYHL
                     FKQGDPLFMRLCTLHNLRFMVQLTERIREQAAHG"
     gene            complement(26823..28070)
                     /locus_tag="PLW25_01125"
     CDS             complement(26823..28070)
                     /locus_tag="PLW25_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS53120.1"
                     /translation="MKRVNGWYLFVLNAYWIGLSFMWNSLHVTILPAVLLKYVPDTQK
                     NTWLGLLTFFGLIIAMLVQPVSGAVSDHWRSKLGRRRPLMWFGTAGDLFFLLLMGFIG
                     GLPALFIGYIGLQTTSNIAHGPAQGLLPDEVPAEKLGLASGIKTALDMAGIVLSSLLM
                     GFLISSSDPNPRLAVTVIIALLVVFGLITLVFTKESPSIGEGRNHLQLGRAIKETFNF
                     KVVGDKHYWQLILSRFLYLVGIYGFQSFAQYFIRDKFPEQDPIMFTQFVMGTFVVVLI
                     IFSLLAGTLSDRYGRKKLQILSSIIGGIGAILLIFAKTPAQLLIYGGFMGVGLGIFLS
                     VNWAIANDMVPKDNAAKYMGLTNIATAGSAAIARLEGPMTDALNNAAPGQWWGWTALF
                     AISALLMFLSAGALLKVPETRKA"
     gene            complement(28110..28745)
                     /locus_tag="PLW25_01130"
     CDS             complement(28110..28745)
                     /locus_tag="PLW25_01130"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOS53121.1"
                     /translation="MAHLPTDHSDVPIRLFKSDFLEFFSHISPITVLVIWLPVALFFL
                     IWSIAHAPAGGFPVVIPLGFLVGLFLWTLAEYVLHRFLFHMPVKGEKAERIIFLFHGV
                     HHAQPQLKTRLVMPPVISIPLAAIFYALFYLFFAVILKAAQWVAPVFSAFIFGYLLYD
                     LTHYATHHFPMRGGYAKYIKRYHMQHHYKTPNARFGVSSPLWDKVFKTMPE"
     gene            complement(28895..30454)
                     /locus_tag="PLW25_01135"
     CDS             complement(28895..30454)
                     /locus_tag="PLW25_01135"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOS53122.1"
                     /translation="MKDHLLAIDVGTQSTRAMVFDLRGNLLAISRVPIEPYYSPAPGL
                     AEQDPQVFWKALCEACNSLWQQPGVAKENIAGVALTTQRSTLINVDKEGKPLRPAIHW
                     LDQRRTSDLKPVGGLWGLLFKIAGMSETVAYLQAEAEGNWLQKHQPEIWSNTYKYLLL
                     SGYLTMRLTGRFADSVGCLVGYLPFDYKKQDWSGKNDWKWQAVPVNKEMLPELVKPAE
                     QIGVITAEAAAETGIPEGLLLIAAAADKACEVIGAGCLNPAIPCLSFGTTATINTTLD
                     RYVEVIPLIPPYPSAVPGSYSLEIQIYRGFWMVSWFKREFGLNEERLAAQRGVAAEEL
                     FDDLVNAVPPGSLGLTLQPYWSPGLKVPGPEAKGAVIGFGDVHTRAHFYRSILEGLAY
                     ALREGAERTSKRTHVPIEEVRVSGGGSQSKAAMQLTADVFGLPASRPHVFETSGLGAA
                     IDAAVGLKLHSDFSSAVKEMTHKGETFEPKQENYAIYNELYRRVYLRMYSHLKPLYDE
                     IRAITGYPPKD"
     gene            complement(30456..>31781)
                     /locus_tag="PLW25_01140"
     CDS             complement(30456..>31781)
                     /locus_tag="PLW25_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOS53123.1"
                     /translation="YSNQHQIPVYVYGAGSSVTRGVEAVKGGIALDMSTHMNRVVSFN
                     ETDQTITVETGMTGPQLEEILNHAPERLGAQRRYTCGHFPQSFEFSSVGGWVVTRGAG
                     QNSTYYGKIEDIVISQRYATPRGELKTCPHPRSANGPDFDQIMMGSEGAFGVLTEVTL
                     RVFRLQPGNRHRFSYMFKNWDDALAACREIMQCECGNPSVFRLSDPEETDVAMKLYHI
                     EGTPADTVLRLSGYLPMQKCLLLGMTEGEAGFSRNVNRQVRRICRRHGAFNISFAPVT
                     SNWEKSRFRDPYMREDLQDFGVLTDTLECAVTWSQMKEVHASVRGFIKSHPNTICMTH
                     LSHAYPQGGNLYFIFIAKIATIKQYLELQYGILSAIQQSGAAISHHHGVGKQTAPWLE
                     GQIGSAQMDIIRALKAHFDPHNIMNPGGTLGLDMTTEQKEQRWSKDLEG"
     assembly_gap    31782..31881
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(32055..33644)
                     /locus_tag="PLW25_01145"
     CDS             complement(32055..33644)
                     /locus_tag="PLW25_01145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOS53124.1"
                     /translation="MWEKGWRQRAWQELDGKWDILIIGGGITGAGILRRAVQEGYRTL
                     LVESGDFSSGTSSRSSKLIHGGFRYLRNKQFRVTRESVREREWLLKEARHLVTPLGFL
                     MPCPDDRKLTSQFAVGVILYDLLAPKWHHSRLNSHDMLDRCPALDADQLHCGFLYYDA
                     GMDDACMVLRLIRESVSAGGCAINYARAAALLKETNGRVCGAVIKDTATGGFQEKEVL
                     ARVVINATGPWSDEVRAGVNAPARLRKLRGSHLVFSRQRLNLPYAVTLLHPRDRRAMF
                     AIPWEGVSLIGTTDLDHTLPLSQGEPYTTQEEIDYILEAANATFPGLQLDRADIISTF
                     SGLRPVVNTGQADPSKESRAHVVWEENGLVTITGGKYTTFRIMAEDALRAAMPALPHP
                     ANLSRPKSYFSPLPTRSSPQGMAAETWLYLLGRYGNETIQLFENAEEGENQPVGSLPN
                     LWSEVRWAARCGAVEHLDDLLLRRVRLGLLLPHGAQEEMARVREIVQPELGWDDARWQ
                     AEFTRYVELYKKAYSPSPAGF"
     gene            complement(33663..34367)
                     /gene="fadR"
                     /locus_tag="PLW25_01150"
     CDS             complement(33663..34367)
                     /gene="fadR"
                     /locus_tag="PLW25_01150"
                     /inference="COORDINATES: protein motif:HMM:NF003444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HOS53125.1"
                     /translation="MIAWSTPQKPAELTESRLISAILDGNFPVNSPLPPERELALSLG
                     VTRPTLREALQRLSRDGWIEIHQGRPTRVRDYWQEGNLLILSAIARHQQHLPADFVPN
                     LLHVRALLAPEYTRLAIRNASSEVIRLLESMQTLPDAAPVFTRVDLELHCRLTILSGN
                     PIFTLILNGFTDLYHSMGSRYFLSPLTRDHSRSYYKKLLSAALSNDIFMAEEVTRAVM
                     RESLELWQEIEGQTSS"
     gene            34635..35084
                     /gene="tnpA"
                     /locus_tag="PLW25_01155"
     CDS             34635..35084
                     /gene="tnpA"
                     /locus_tag="PLW25_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044282811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOS53126.1"
                     /translation="MSETWRKSHNISILIYHFVCPAKYRKVVFNDEVDEVLKNTCLEI
                     EKRYEIKFIEIGTDKDHVHFLIQSVPMYSAKKIIQVVKSITAREVFAQVPEVKKKLWG
                     GEFWTDGYYVSTVGQKGSEETIRKYVQEQGIEKDYKHLHYDEQLKLF"
BASE COUNT         7727 a         9377 c        10486 g         7357 t
ORIGIN      
        1 ttcattcatt tttattttgc aggagtaatt atcatgtcgg tcgattttat ttttcgtatt
       61 cttgggatgg tcatcctggc gattgtgggg ggaattctgg gtaaccagtt tggagaaatt
      121 gccaatacat ctccaagcgc caccactttt ccggtggagc agtatcgctt tctgtttgga
      181 ttgctgggag cgttgttcgg gctcatcctc acgccatatt tcaccacccg cccagcgcgg
      241 gcgatgcgca acgcgctggc aaaagccacc actcatgctc tggtggcctc tttatttggg
      301 ttggtggcgg gtttaattgt ggccgccttg ctggccttcc ctctttctct gctgcccgat
      361 ccatttggca gcgtgctgcc ttttgtgggc gcaatccttt ttacctactt aggcgtggtt
      421 atttttaagc tgcgacaaca ggattttctc aatttgctct ccaacctgcg tagcggttca
      481 ggagtcacgg cgaaagatgg ggcatcaggc gcgggctggg cggaaagccg caccatctta
      541 ctggatacca gcgtgctcat cgatggtcgc atcgcggaca tctcgcgcac ggggtttctg
      601 ccaggctcgc tgcttatccc gcgctttgtg ctcaacgaat tgcaatacat cgcggattct
      661 cctgataata tgcgccgtca acgcggccgc cgcggtatgg aggtgctttc cgcgctgcaa
      721 aaagaacccg ccatccctgt gcgtatcagc gatatcgatg tggaaggaat gcgcgaggtg
      781 gatgataaac tggtggtgct ggcgcggcaa atgcgctgcc cgatccttac caacgactac
      841 aaccttaacc gtatcgctga attgcagggg gtcacggtgt tgaacatcaa cgaactggcg
      901 aacgcggtaa aatcagtgct catccccggt gaaatcatga ccgtacaggt cattcaagaa
      961 gggcgcgaga ccgggcaggg tgtggcgtac atggatgacg gcaccatggt tgtggtagaa
     1021 aatggccgtg aatacatgga taaatccatc caggtgacgg tcaccaaggt attacagacg
     1081 gctgccgggc ggatgatctt tgcccggccc gatgaagcag cgaattagac cccgaacagg
     1141 atttcatatg aaagtttaca acacactatc ccgcaaggtt gaggaattcc agaccctcga
     1201 acccggcaag gtgaaaatgt acgtctgcgg ccccaccgtg tatgccaagg cgcatatcgg
     1261 gcatgccatg tctgcgctgg tcttcgacat cattcgccgt tatctcgaat atcgaggata
     1321 tgaagtacgc cacgtgatga atttcaccga cgtagatgac aagatcatcc tgcgcgccca
     1381 agaaaagggc gtggacccat tcaaactggg cgagatgtat attcatgaat ttgaaaaaca
     1441 cttacaggat ttcaacattt tgccggctac cgtgaacccc cgcgccacac gggaaatgga
     1501 gcagatcatc accatgatcc agggattggt ggagaagggg tttgcctatg acaaagatgg
     1561 cgatgtgtat ttcagggtca ccagcgacag ccagtatggc aaactctccg gtcggcgcat
     1621 tgatgacatg cagtcaggtt accgtataga ggtggatgaa cgcaaagagc accccatgga
     1681 ctttgcgttg tggaaggcag ccaaacccgg agagcccgcc tgggacagcc cctggggcaa
     1741 aggtcgcccc ggatggcata tcgagtgttc tgccatgaac ctcaatcacc ttggcgaaca
     1801 gattgatatt catggagggg gcaatgacct ggtcttcccg catcatgaga atgaaatcgc
     1861 ccagagcgaa agttataccg gcaaaacgtt cgcccgttac tggattcaca atggtatgct
     1921 gcaactgagc ggcgagaaga tgtctaaatc ggttggcaac ctggtaacca ttgaagaatt
     1981 ccttgagaat cacccggcag acgcgctgcg catgctggtg ctgaattccg gctaccgcaa
     2041 tccattgtct ttctctgatg agatactcgc gcaggcagag aaagccattg aacggctccg
     2101 ttctggtctc aaacccgcgc ctgcggcagc gcccggggct gctcaatcag tagtggacgc
     2161 cctggcgcgc caggtggaaa cggcagaaag cggctttgtg caatccatgg atgatgattt
     2221 caattccgct ggggcgctgg cgcaactttt tgacctggtg aggggcatca atcaggcgcg
     2281 cgcggatggc gccactcacg cgcagctcag cgccgctcag ctcaaattgc gccagcttac
     2341 cgccgtgtta gggctgagcc tggaaaaaga gaccgaaacc caccaggaag tcgatggttt
     2401 tgtgaattta ctcattgaaa tccgccgcga attgcgcgcg aacaaactat gggaactctc
     2461 agacagcgtg cgcgaccggc tggccgcgct gggggtggtg ttggaggaca gtaaggaagg
     2521 cacctcctgg cattggaaat gaccggcaaa tgaaagaatg gattacgggg cgcaacccgg
     2581 ttatggaagt gatgcaggcg aaacgccgtc aggtgtttcg cctgttgctg gcttctgggg
     2641 tggaagaaaa agggcgcatt gctgaaatac tgcgcgcagc cgcttcaata aaagctccag
     2701 tcgaacgcgt cgcgcgagaa cggctggagc ggcttggtga aaacccgcag ggggttgctg
     2761 ctgaggttgg cgtctaccct tatgttgagt tatatgacat ttatgacctg gcgaaagaaa
     2821 gaaacgaagc gctgttcgtg ctggtgctgg atgtcattca gaaccctcag aatttaggca
     2881 cattgctgcg caccgctgaa gcagtgggag tgcacggagt tatcatcccg cagcaccgtg
     2941 ccgcagaggt aacgccggca gtggtttccg cttcggctgg cgcttcagag cacttgctca
     3001 tcacgcggag caatatcgcc cgcagcctcg agcagctcaa agaagccggc gcctgggtgg
     3061 ttggcctggc ggaaggggag gacgcgccag ccgaagtacc cctgggcggg cccattgcgc
     3121 tagtggtggg gaatgaagga gagggcatgc gcgcgttggt caggcaacag tgtgactata
     3181 tcctcagcct gccaatgcgt ggacgcatcc aatcgcttaa cgccgctgtt gctggctcga
     3241 tcgtgctcta ccaggtgttg cttgcgcgcc agaaaacctc acgctgaccg tgttgttcca
     3301 cgtacggatg ttcgttcatc acctgctggt ggtataggat aaccgcgcaa gtacctggga
     3361 agccgccagg gcggccgtgt tggcgggttg gtttctgacc gtctttgata ttcctggaaa
     3421 gaagcggtgg gcgatgtatg gtttcgccaa gcagttccaa cgtcctctct gttggggttt
     3481 tgcagcgggc atccttactg gtcacggctg tattaaataa acgaggacag cgccatccag
     3541 ctgctcccgt tcctgaaaga tgtgcaagta cttttttaaa gccagagcac ccagaccacc
     3601 cacaagccaa tcatcaggac gccgattccc aaacgggcga tggttgcgaa accgcaccct
     3661 ctgatgatcg ccccggctga ttttgcgcct tccttaagat ctcgtttacg tacccattct
     3721 acaaccagta tgctcagcgc ggcaaaaagc agcccgccaa agggcggcaa aataaaggtg
     3781 ccggcgatcc cagcggcgag cgctaccacc accgatatcc aactggcgcc ggtcttcttg
     3841 gcttcagcgc ccatgaataa gttgtcaatg aggttgcccc ctatcatcag cagcgtgatg
     3901 accgcaaaca aaatgccggt ggcaggtgaa aaaccgctga cgatgccgta ggcaaggatg
     3961 gccagccaga tgatagtgag cccagggatg atatagagca tcagaccgaa cagaccaacg
     4021 cccatgacca ccgcgagcac gggcacaaac cagttggaat tcgcaatggc atccaatttt
     4081 aagaaatcac tttcaacccg cccatccagg gcagcagcac ttcaggcact ttaatttttc
     4141 catcagcctg ttggttattt tcgaacaccg cgatgagggt ccgcggcagg cccagtcctg
     4201 acccattaag ggtgtgcacc aggcggttac gccctccttc agcaggtttg tacttgatgt
     4261 tggcgcggcg cgcctgaaaa tcgccgacat tggaaacaga agatacttcc agccactcgt
     4321 tgcaaccggc agcccacact tcaaggtcgt aagtgaccac agaattaaag cccaggtcgc
     4381 cggtgcataa ttgtttaacc cggtacggca accccagcaa cgcacaggtt tcttcggcgt
     4441 cggcgagcat tttctgcagc tcgtgcattg attcttcagg agtgctataa atgtacatct
     4501 caactttatc aaactggtgc ccgcgtttga tgccgcgcac atcccgcccg gcgctcatct
     4561 tctcgcgcct gaagcatggc gtgtaagcgg taaagcgcag cggcaggctg gcctcctcca
     4621 gcacctcatc gcggtacata ccggtgaggg gcacctccgc ggttggcacc atccacaggt
     4681 cttcttcgat gtcgtgatac aggttgtcta cgaacttggg cagctgcccg gcggcgtaga
     4741 gggtttcagc tttgaccatg aaaggtgtat agcgttccac gtagccctgg cgggcgtgca
     4801 gatcaagcat ccatgagatg agcgcgcgct gcaggcgtgc agcctctccg ttgagcacat
     4861 agaatcgcga cccggtcagc tttaccccgc ggtcaaagtc gatcaacccc atcttcggcc
     4921 ccaactccca gtgaggcagg ggggtaaagt caaacttggg cagcgcaccg tgggtacgca
     4981 gtacgatgtt ctcgctctcg tcttttccaa taggggtgga agggtcagga aggttgggta
     5041 tggttgccag cagcgcttgc agtccttcat ccacctgcct ggtggtctca tcaagttgcg
     5101 cgatttcctc gcccaccgaa cgcatggcgt cgatcttctc ctgcctttca gcggggtctt
     5161 tggtctgact gatttccttg gagaccgtgt tgcgcagggc tttgaggttt tccactttaa
     5221 ccagcagtcc gcgcctttca gcgtccatgg ttaataactt gtcgaccacg ctgctgtcca
     5281 tctgccggtc ggtgagcgct ttgcgcagct cctcaggatg ttcccgtatg aaattgatgt
     5341 ctaacatgct gcacctcatt tttaaagaat aacgccctca tctgtcgcgc aggacgaggg
     5401 gcgcctcgtg gtgccacctg ctttcgctcc cgtaggggaa cctcattgtg gtattaacgg
     5461 tgcctccggt ctctttacgg cgagaatgcc cctgcagaga caacctgccc aagaagggcg
     5521 atcgggttgg aatccgcctg ggcgttcctg ccgctttgca gcggtcagcg gctctctgga
     5581 aggatatccc aggcagtagt acccgagctg gtcttgtaaa gtgattatac cttgcgctta
     5641 atgcctgtca agaacaacgc gcgcaagggg atgacagccg cggtcaatcg tcagttaaac
     5701 aggatgatgg aaaaggacaa attgcgataa aatcaattga tcatggacga acaggctttg
     5761 gtcgcagacg cgctgcaagg aaatgtagac gctttcaacc gtctggtgct ggcataccag
     5821 gacatggctt ttaaccttgc tttccgcatg ctgaacgatg atgacctggc ggccgacgcg
     5881 gtgcaaaacg cttttctttc tgcttaccgc aacctgcgcg cttaccgcgg aggttcgttc
     5941 aaagcctggg tgatgcgcat ggttaccaat ggttgttacg acgaactgcg ccgccagaaa
     6001 cgccgaccca cgattccgct tgaaccgcag aacccagaag atggcgagga aattgaatct
     6061 gccgattggc tggcttctga tgacccccaa cccgaacagg cttttgacca ggcggagctt
     6121 gaacacgccc tgcagcactg cctggaaaac ctgccgcctg actttcgcgc ggtggtcgtt
     6181 ttgactgatg tgcaggggat ggattatgaa gaagtctcac gggcggtgcg cacgccgctt
     6241 ggaacggtga aaagccggct ggcgcgcgcc aggcagagac tgcgtgattg cctgcggcaa
     6301 tttcaggaac ttctgcctac tcaatttcgt cttaccggtg gagaacgccg atgactgaga
     6361 gattgaccag cagagactgg atactgattt cagcctacct tgacgggcgc ctttcacccg
     6421 cagccttaac ccgcatagaa aagcgcattc aggaggacgc tcattttaag cgatctctcg
     6481 aagaaatcgc gcgattgaag caattactgg ctgcgctgcc caaaaaacgc gcgccgcgaa
     6541 atttcaccct tgcgcctgaa aaagcgcaaa aaccgctgcg cggattgtgg cttcaaccag
     6601 cgttgagttt tgtgtctgtc accgcgtcaa ttctcctggt gctggtcttt tcctttaatt
     6661 acttaggtat gggcttgccc cgccaggcgg ctgcccagcg ggcggaaatc gccgccatgg
     6721 aaggggttgc tgaggctcct gcgccaatga tcatcaattg gatcccgtca ggcggacagg
     6781 gcggaggagc agcagaagcc aagagcccct atacagggat cgatggtatc ggagggccgg
     6841 gttttcctgt tgctgaaccg ggtataggcg gcggaccagt tgaaccggct gttcctgaat
     6901 tacctcccgg cgttgaccca cagcccgtga tgaacgagga gcctgagccg cctgcttatg
     6961 atgaacccgc gcccgccgca ctggagggcg aaccggtttc agacgctgac ctttctaacc
     7021 tgatacttgg tctcgcggaa gacgacacag ccggtcaggt gatcgaaagc gcgccgctgc
     7081 gcaaagcacc ggcgccgcaa cctcgccgcg ggttttccgc gcccagcctg gttatgttga
     7141 tcgcgggcat taccgctctc ctttcaggcg caggagcgct cttcctccgt aaaaaatttc
     7201 atgcatagcc attcgcacga acatttcaaa ttctgtccgc agtgcgccac accgctgcgc
     7261 atgatgatgc agctcggaga gcgccgacct tcctgccccg cgtgcggttg ggtgcactac
     7321 gaggatccca aagtcgcggc gggtgtgctg ctgctgcgcg ggcgggaagt gctcctcgtg
     7381 cggcgcgtta tggagcctta cctgggtacc tggagcattc cggcagggtt tgtcaacgca
     7441 tttgaagacc ccgcggcggc tgccttgcgc gagtgccggg aagaaaccgg gttgactgtt
     7501 gagatcgaag ggttgttcga cctgttgacc gggagagaac acagccgcgg cgcggatatt
     7561 ttcatcgttt accgcgcgca ctttctcagc ggctctctcg ccgccgctga tgacgctgat
     7621 caggttggct ggttccctct gtttgacctg ccccctttgg cttttgaaac cacccggcga
     7681 gtcctggaaa aagcccgcca cctccagctc tgagttacct cctgctggcg agaacaggcg
     7741 cgttcccaac tcttgctcat tcgaataatc accagttggc ttgacctttc ggagggggaa
     7801 gtgatggttc gcgcgcagca catctatctt cgccactgat gggcgcgcaa caggttttta
     7861 tccatcggga ttacccgtcg aacaagggac tgggctgaat gctaagatgg tatgaaacct
     7921 gcatccgttt gatgagaatg gaaataacga atggccaaaa gcacgaaaac tcgaaaatct
     7981 tacgctcatc cgcctaagtc ctcaaggtct tcctccagaa gagggcagta ttcgcgttcg
     8041 accctgaccc tggaccagcg cctgaacttc atcggcgggg tnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nagtattcgc gttcgaccct gaccctggac cagcgcctga
     8221 acttcatcgg cggggtgacc gttttgaccg gggtactttc gatcgtcgcc ttattttcca
     8281 atgccaatgg tccgctcagt gggtggatcg cgcaaaccac cacccgcctc gctggctggg
     8341 gtggcgtgat catcccgctg gcattgatca ttggcggcac ggtcctgctg ctgcgccatt
     8401 atgagaatct cccacgggtt tcttactggc gcctggcggg catcattttg ctttacctta
     8461 atatcttggg ctggtttcat ttcatagccg gcgggggttt ttcgaccgcg aaagacgggc
     8521 ggggtggcgg gtacatcggc gctttgtttg accgcgtttt agccaattct ttgggcccgg
     8581 ctggagaggg ggtttttctc ttcgcctggt tactggtggc cattttgttc atcctcaacc
     8641 tcccgttggt tgaaatcgcc gcccgtgtga acacgctgct ggtcagattt aagaaaccga
     8701 aagaagagaa cccgcgccaa cccatgttcc acttcaccag cgccaggcct taccgggtaa
     8761 aaaatgaatc acgaacagac ctgccggatg gttataaacc gctcgtggtg gaggaagaaa
     8821 gcccggcaac ggtgacggtg atcgggagcc cctcagcggc ggagccaaaa acaaccgggg
     8881 tcagagaaaa gcctgcccgg gttgcgcctg agccctctga aagccttccc gtcacccgca
     8941 cacgggtgac cacgcccgtg gcaccctggc caattccagc ggtgagcgat atccttgacc
     9001 tggcgacccc agcggttgtg caatctcact acgaccaagg ccgcgcccga attattgaag
     9061 aaacgctggc ttcatttgac gcccccgggc gcattgttga aattcagcgc gggcccgcgg
     9121 ttacccagta tggcgtggaa cctgagttca ttgaaagccg cggcgggcgc acgcgcgtgc
     9181 gcgtgtccaa gatcgccggg ctggcgaatg acctggcgct ggctctctcc gccccgagca
     9241 ttcgcataca ggcgccggtg ccgggtaaag cctatatcgg cattgaggta cccaactcgg
     9301 agctttcact ggtttcgctg cgcgagttga ttgagacgga cgcttataag aaaatcaggt
     9361 ccagcctgcg gtttgctttc ggcaaggatg tggcgggcaa acccatttgc gccgacctga
     9421 cgtctatgcc ccacctgctg atcgccggca ccaccaattc aggcaaatcc gtgctggtca
     9481 atgccattct caccagcctg ttgctcaata acagcccgct cgacctgcgc ctggtgctgg
     9541 tggaccccaa gcgggtggag ctgaccgggt ataacggcat tccgcacctg ctcgccccgg
     9601 tggtggtaga cgcggaacgc gttgctggcg cgctgcaatg ggtgctgggc gaaatggatt
     9661 cgcgctacag gcgctttgct gaagagggcg tgcgtaatat catggatcac aaccgcaaag
     9721 aggctgagcg cctgccgtat attgtggtgg tgatcgatga gctggctgac ctgatgctgc
     9781 tggcgaaaga tgaaactgaa cgcagcatta cccgcctggc gcaactggcg cgggccacag
     9841 gcattcacct gatcatctcc acccagcgcc catcggtgga tgtgatcacc ggggtgatca
     9901 aggcgaactt gccggcgcgc gtagccttca tggtcgcctc caacctggac agccgcgtaa
     9961 tcatcgacaa gcccggcgcg gaaacgctgc tcggacgcgg cgatatgctc tatcaggcgc
    10021 cagacgcccc cgcgcccgtg aggttgcagg gtgtgtacgt ttcagaccag gagattcaac
    10081 gcctggttaa tgcgtggcgt ctcatcgtgg ccaatcgccg cgctgaaggc gggaaggaac
    10141 tctttaccgc cacctttgag taccagcccc agcacgtgcc gtttaaacaa ccctcatttt
    10201 ttggggatgg cgcccaggag gatgacccgg ttttaaagaa cgccatcgaa atcgtgcgca
    10261 aagaaggtaa agcctccacc tcttttctgc agcgccgttt aggcattggc tatactcgcg
    10321 cggcacgcct gatcgatact ctggaagagc agaagatcat cgggccgcgg caaatgggat
    10381 cgcaggttcg cgaagtgttg gattttggcg cggacaatga agagcccccc gctgaaacgc
    10441 aatcggattg acccagaaag acagccgctc attttccgtt ctccccagag aataaaaata
    10501 gacaggttca tttggccggt ttttcagtat aatatttttt gaacactccc tgtgagccag
    10561 caatacacgc gaccgcgcca gccatggaaa ggacgggtgg ataatcacat aaccggtcgt
    10621 gacaaacgag gagaggatac ccgatgcgca agcgcaattc tttttttctt ttaattgccg
    10681 ttttattgct ggggttcttg gcatcttgcc gctccgccag gctaccgttg cccgcctttt
    10741 tatcagcgga tacgcccacg ccaaccgcga ccatgacctt cacccccacg tacacccccc
    10801 tgcccacgaa aaccttcacc cccacgccca cgccgccagt gaacttgatc ccctgcgcca
    10861 attctgatgc ctgttcagag ggcgtataca tccgtgaata cctggagacg gtcggcctat
    10921 tggacgaagc agacgaggga gagcgaccgt ttgaagtgga ggtgccttac aaccttcctc
    10981 tccgcttcgg ccttagctgg atcgccatcg atgatgagac gctggcggat aacatgaaga
    11041 agatgacctt tttctttgag attgatggac agccttacct ggataagaac gatctgatct
    11101 acgaatacta ccctgatgtg gaagaccctt ccatagtcta ctcttcagtc tttattgatt
    11161 atttgttgac tggctggagg atcggtgaac ctcatatggt cagcattggc ttccgttttg
    11221 atcaatcgct ttcagacggc tggaacacgt attacctcgg tgatacaatc gggtacagtt
    11281 accgcatcct gcctgtggag ctgcccaccg ccacgcctac cccttcccct actagcacac
    11341 cccagccgcg acccaccgcc gtgccaccca ccccaacggt ggcgtgcgag ttgggcacca
    11401 cgattgaaat caaaaatgac accggcggac aggtaaccat gcacttcacc ggtccggtca
    11461 agtacacctt ttacatcgcg cccggctcgc gcacgctgca gctctgcact ggccaataca
    11521 gctacacggc ttacggctgc ggcggatctt cgacgagcgg cagcgcgggc gatggggatg
    11581 aaatcgagtt ctggtgtgag tgacccgatc gcgctttttg tgctgtccgt tggtgcgagc
    11641 gagaccgcgc tcaggggttg gtgctgcgat tcctgtgccg aacggcgcga acgatgagca
    11701 gcaccgccag cacgataaag aacccagcca ggaagaatga gcacatcagc accgccagcg
    11761 cctgcagcgt gcgctcttgt acctgctgac cagagctatc cacgcagtag gcgcgaaact
    11821 gggtgctcgc ttcctcctcc caatctccct cctcctccca ctcctcgaat tgcatctcgc
    11881 tgccgggcgg gcaaaccacg ccggtggtga gggcgaagag cgggcgcgtg aaaatcaccg
    11941 ccagcactcc cgcgggtgcg cagaaagcca ccatggcgat gaacgccgat atgatcacca
    12001 gtttcagcag gctgctgcgc ggtgcctgca cattcggcgg ttgcaaagga tgattgttca
    12061 tacttttcat tatgacacaa aaaggcgttt ttttaaccct ttgattggcg catcgtcccg
    12121 gcggggatta tggctcaggt ttccctttac ttatcataga caacccaaag aagaataaca
    12181 atgacagccg aagagaaagc aacaagggac aaacaacaga gagtctcgtc aatgactctc
    12241 tgttgttgaa agaagattga ttgatctaca agcccgccgc gcttctcaat aacccggctt
    12301 tgtctgtcct ctcccaggtg aactcgggca gctcgcgacc gaagtgcccg taattggagg
    12361 tcttgcggta gatgggacgg cgcaggtcga ggtcgcggat gatggcgccg gggcgcaggt
    12421 cgaagtgctc gctcaccagc gcggcgatct tctcatcggg gatgacgccg gtgccgaagg
    12481 tctcaacatt aatcgagagc gggcgcgcca cgccaatggc gtaagccaac tggatctcgc
    12541 agcgcgtggc gagcccggcg gcgacgatgt tcttggctgc ccagcgcgcg gcataagcgg
    12601 cggagcggtc caccttggtg gggtctttac cgctgaaagc gccgccacca tggcggccca
    12661 tgccgccgta ggaatccacg atgatcttgc gcccggtaac gccggcgtct cccatgggtc
    12721 cgccgatgac gaagcgcccg gtggggttga cgtagatctt gggcgtgccg tccagcagct
    12781 ccgcgggaat ggcggggttg atgacgtgct cgatgacgcc ctcggtgata tccgcgtgcg
    12841 aaacatcagc ggtgtgctgg gtggaaacga ccacggtatc cacgcgcact ggcttgccgt
    12901 aagcgtactc cacggtcacc tgcgacttgc cgtcagggcg cagccagggc aggaggccgg
    12961 ctttgcgcac ctccgccagg cgctgggtaa ggcggtgcgc caggtagatg ggcatgggca
    13021 tgagggtggg ggtttcatcg caggcgtaac cgaacatcat cccctggtcg ccggcgccga
    13081 tatgctccag ctcggtttct ttgtcctcgc cgcctttggc ttcgtatgat ttattcacgc
    13141 cgagggcgat atcctggctc tgcgaagaaa tggcagagag cacgccgcag gtattaccgt
    13201 caaaaccttt atcagaagaa tcgtagccga tgctgttgac cacctggcgc accaggtcgt
    13261 tgaagttgac gaaagcgtta gtggtgatct cgccgaggat catcacgtag ccggtcttga
    13321 cggcggtctc gcaggccacg cgcgagcgcg ggtcttgctc gaggcatgcg tcgagcacgg
    13381 cgtctgagac ctggtcacag agtttatcgg ggtgcccttc cgtgaccgac tcggaggtga
    13441 atttaaaact ggggttttgc atgaaagaac tgcctgacat gggtttttct ccttataaaa
    13501 ataagatgtt gccccagacc ggtgaactcg aggggcaaca tgagcgtttg gctgccctct
    13561 atcttccaga ttgtttccct gccggaattg gcaccatgtc gcgagttacg ctcctggttg
    13621 ccgaggcttc atcgggccgg tccctccgcc tctctggata gaagtgccgt ttttaggggc
    13681 tattcaatta aggtcagttt atcacgggga taggcgggtg tcaaatgaca aaagacatat
    13741 tcttgcgtat caggataagg cgcggtacgc ccgctggggg ggttatcccg gcgtcgcgct
    13801 gggttcttcc gtgggctcag gaggctcctc cgtcggctca ggggactccg ctgtgggctg
    13861 gctggggggc ggcgtgttca cctgggtggt gctggtgggt agggtggtgg gcggcggcgt
    13921 ctgcggggtt tcagtggggg tggagggcag aggggtaagc gtgaccgtgg ggtgaggagt
    13981 gggcgcgggc gtgagcacgg gcagggtgtg ggtatcccca aggacgttga caaaagcagc
    14041 cggcacccag caatcgggcg cgcgcttttt gggcaaacgc acataaaaga aagcgtcatc
    14101 agggctgcgg gcgagcgcct gcagcacatc accggtgcgc aggatgaggt aactctcaaa
    14161 tgagctatcg ggcccagagc ggcagttcac acccagttca acttcgttga ccgtgagctg
    14221 gacaatttgc gagatgggca aggcggtggg gatttcaaga tggctgagca aagaggtggc
    14281 ggtggggctg ggctgctcag ggcgcggcga aggcgtgcgc gtggggaagt cgtagggctg
    14341 atagggtgtt tgcgtatctt cgggcacata cgcctggatg atgtgagaga tgcgtgccgg
    14401 tgcgccgagg aggaagaacc cggccagcag cccagcaccg aagaccgcca gcgcggactt
    14461 gatgaaaatg gcgcggaaaa ccgccgggtt ttgtacccag cggaggctgg cgggcaggcg
    14521 cgcgggggcg gcttgatgaa ggatggggca atcgcgatag cgctgcgaga ggcagtactc
    14581 ctgttgctgc tggcgtgaag gcacgcagac cgattggcaa tgcaggcagt ggttgacctc
    14641 tgaagggaag gcggtggtgg ttgtggtgtc atcgagcatg cccaggtgcg ggcaggtgta
    14701 aaggtcgtgt gcggttttcg cggaagaact gctggagctg ctcataccct gaccggcggc
    14761 gattcccggt tttcccctct ctggcgcact ggcgaaacgc gcgcccagga taaattaaaa
    14821 cgtgtccaat taaaagatgt gaatattgac ttcgctgtta ataagatacc ataaatcgta
    14881 gtgcgtgtat atcaggctaa ccttaaaaaa agcgaggagc gaagtgatct ttcagcgttt
    14941 ggcagccgcg cctgcgcgga cgcggtttgc tcaggcttct ttgcggatgg tgtaagaatc
    15001 aaaatcgcgg gcgacgctgg tgttttcgaa taccgcctgg gcctcagcga ggatatcttt
    15061 atcgcggtag cggcggctta gatgagtgag aatgagcttg ccagcgccaa tgctcgcggc
    15121 gaactcgccc gcctgacggg cggtgaggtg ggcgtactgg cgcgccatac tggcttcttc
    15181 ttccagatag gtggcttcca ttaccagccc gtcaacgctg cgcagcacct cggtgagcga
    15241 agcggtttcg ccgaggtcgc cgagggcggc caggcgggcg ccgggtttaa aggggccggt
    15301 gaccatgctg ggggtgataa cccgcccgtc gagcagggtg acgctttctc ctttgaccag
    15361 tttgccgcgc agcggcccgg cgggcacgcc cagcgcgtcg gcggcttccg ccaggaaggg
    15421 ctggcgtccc ttctcctcga agagatagcc gtagttatcc ggtccgcggt ggttcacagc
    15481 gaaggcgctg atggtgaaag aaggagcttc aaagatcacg cccggggtga ccgggatcag
    15541 ctccacaggc attggagccc tggcgccgcg cagcaccacg ccgtgcacca ggtcgctgat
    15601 gcgttcgagc gcgttagcgc cgccgtagat ttccaggcgg tcaatggttt cccaacgcat
    15661 gaaggtggag agcaatccgc caagcccgag gatgtggtcg aggtgaccgt gggtgatgag
    15721 gatgtggttg agacgcttga agccgatgcc cgcctgcagg atctgccgct gggtgccctc
    15781 tccgcaatcg attaaaaagc ggtattcgtc gtgctgcacc aggtaggcgg gaagcccgcg
    15841 ccggggggag ggagcagagg cggaagtacc gaggaagatg atctcaaaca atggcgcggt
    15901 cctttcaaca ggtggtttta ttttacccta agttaagcgg cggtcgggaa tggcacgaag
    15961 cttgcaagga tgagggcacg gagaaggatt cgccgccggg tgtctgaacg gctgttcagc
    16021 ccgcgcgcaa aacgaagcga ggtgaatatg gaattcttaa gcgtgagcat ccacaaaccc
    16081 gaagcgctca atttcattct cgggcaatct cattttatta aaaccgtgga agacatccac
    16141 gaggcgctgg tgacggcggt gccgggcatt cagtttgggc tggcgttttg cgaagcctcc
    16201 ggcgcctgcc tgatccgctc atccggcacc aatgaagcgc tgacgcagct ggcgcgcgaa
    16261 aacgctatga acatcggcgc ggggcactgc ttcattttga tgttgggtga aggttatttt
    16321 cccatcaatg tgttgaacct ggtcaagaac attcctgagg tgtgcgcgat tttctgcgcg
    16381 accgccaatc ccactgaggt ggtgatttac gataacgggt atgggcgcgg catcatgggc
    16441 attgtggaag gcgaaaagcc caaggggttt gagagcccgg aagacgtgcg ctggcgtgtg
    16501 gactttttac gtcgaatagg atacaaggcc gggtaacgcg caggcgccgc ctggaacaca
    16561 aggagccaaa gatgaaaaaa ttaattcctg tactgatcat tctcagcctg gtgctgaccg
    16621 cctgtggcaa cttaccgctg ggacgaacgg aagaggacat gagcgatagc gagatggcca
    16681 cgcgtgtggc ggaactgctt tcaaccatga ccacccccac taccgagatg gtctttccgc
    16741 ccacgcccac gccgcaggcc gccaccgagg ctccgcctga gcccagcgcg gcacccgcca
    16801 cgcaggagcc ggcagcgctg ccggcgaccg agacccccgt ggtggtggag gaaaccctgc
    16861 tccccgagga agccggcacg cccacaaccg aggtgacacc agcgctgccg gaaaccgacc
    16921 cggcgcgcaa actgggcaac cccacccaca gcgacccgct ggacagctat gagaagtgga
    16981 cctggccgac aggcagcgat gattacctca aggtaaactt taaagacggc tttctggaaa
    17041 tgaccgggct gaccaccatg gcgggctggc gcctgccgat actggcgcag cagaccaaca
    17101 gctacattga acttaccgtc aactcaggcg cctgccaggg aaaggattcg tacggcatca
    17161 ttttccgcgt gccagtgttc aaagaaccga cacagggcta cttgtatgag gtcacctgcg
    17221 atggttacct gcgcctgtgg gaatggaatg gtaaagccaa acccaaaggc gaggctgacg
    17281 ttctgatcaa ctggaaagag tcgatggata ttaagaccgg cgccaaccag gtgaaccgcc
    17341 tgggagtgat ggcggtgggc aaaagcctgc gcatctatat gaatgggatt ccgcagggcg
    17401 aggtgagcga ttcgacctac ccggcgggat ttgtcggcgc ttttgtgcgc tcggtgacca
    17461 cccccaagta cacggtgaaa tttgacgaaa tgaaggtctg ggaaaacccg gcgcagtaac
    17521 ggtgtacgga aggaaatgga gcgcggagcc gccaggggcg gctccgcgtt ttgtgttgcc
    17581 tagagatttt ttcgctgctg cgcgaaaaca gcattcaggc taaaatcaga cggtaaggag
    17641 tggacgaaat gaaaagttac cgcaaagaga tcacgctcaa catccccgcg cggcgggggt
    17701 ttgtgaacat cacgccgcag gtgcaggcct gcctgcgcga gagcggcatc agcgaagggc
    17761 tggtgctggt gaacccgatg cacattaccg cttcggtgtt cattaatgat gacgagagcg
    17821 gtttgcaccg tgattacgac gcctggctgg aacgattggc accgcatgaa ccggtgaacc
    17881 agtaccgcca caacgacacc ggcgaagata acgccgacgc gcacatgaaa cgtcagctga
    17941 tggggcgcga ggtggtagtg gcggtaaccg gtgggcgtct ggactttggc acctgggagc
    18001 agatctttta cggcgagttt gatgggcggc ggcccaagcg cgtgctggtg aagatcattg
    18061 gtgagtgaga tgagcaaccg cgtttatttc ggcgaaaatc tcgaggtgct gcgcgcgctg
    18121 cccgacgcat cggtggagct gatctacatt gatcccccgt tcaacaccgg caaggtgcag
    18181 gcgcgcacgc ggctgcgcac cacgcgcgca gagaatggcg accgggtggg ctttcagggg
    18241 cagcgctacc agacggtacg gctgggttcg ctgggctaca atgacatttt cgatgatttt
    18301 ttggctttca tcgaaccgcg cctgttggag gcgcaccgtg tgctcagcgc cgcgggcagc
    18361 ctgtattttc acatcgatta ccgcgaggca cattattgca aggtgctgtt ggatagtatt
    18421 tttgggcgcg catgtttttt gaatgagatc atctgggcat acgattacgg cgcgcgcacg
    18481 cggcgtaaat ggcctcccaa gcacgataca attttttggt acgtcaaaga cccgctgcgt
    18541 tacactttca attatgacgc cattgaacgc attccctaca tggcgccagg cctggttgga
    18601 ccggaaaaag ccgcgcgcgg caaaatcccc acagatacct ggtggcatac catcgtgccc
    18661 accaacagtg gtgaacgcac cggttacccc acgcaaaaac cgctgggcat tctgcggcgc
    18721 attgtggctg cctcatccaa ccccggcgag acggtgctgg atttctttgc tggcagtggc
    18781 accaccggcg ccgcctgcct ggaactgggg cgcaggttcg tcctggtcga taacaatccg
    18841 caggcgctgg cggtgatgac tgaacgcttt aaaggcgtaa cggatatcga atggatcatc
    18901 gacacgcctg aagccatgga ccgatgaaag aatatcttct ggcgcgcccg cagcccggtt
    18961 agcacgaacc ggcagcggtg ttttcgccca cgcggcatgt aaacacgggc acgcctacct
    19021 gtgagccgcg cagcctgcgc gaccccgagc tggcggtgga gacggccggg tttatgtggg
    19081 tctcaagcag gttgaaacga agactacctg aaggttgggt tgagggcgcg cacctgttcc
    19141 gcggcgtggt aagaagagcg caccagcggg ccgctttcta cccagccgaa gccgagagat
    19201 aacccgtatt ccttaagatc agcgaattct tccagtgtgt aataacgttc gatgggcagg
    19261 tggtgccggc tgggttgcag gtactgcccg agggtcagaa tatccactcc ccagtcgcgc
    19321 aggtcgcgca tgacggcttt caattccgac atggtttcgc ccaatccgac catcaaaccg
    19381 gatttggtca gcacatccgg ggcgagcagt ttggcgtggc gcaaaacggc ttctgaccac
    19441 tcgtagtggt cctgcggctg cactttcttg aacaaagagg gtacggtttc cacgttgtgg
    19501 ttgagaatct cagggcgggc gtccatgacc atttttaacg ccgggaggct ggctttgaaa
    19561 tcagggatga gcacttcgac tgaacagcct ggcaccagct cgtggatacg acgaatgacc
    19621 gcggcaaaga tgggggcacc gccgtcttcg cgctcatccc ggttgaccga ggtgatgacg
    19681 gcgtgcttga ggttcatcgc ttttaccgcc tgcgccacgc gttcgggttc ttcccaatcc
    19741 agcggggcgg ggcgcccgtg cttgacatcg cagaagcggc aggaacgcgt gcaaatttct
    19801 cccagcatga gaaaagtggc ggtaccgctg ccccagcatt ctcccatgtt cgggcatccg
    19861 gcttcttcgc aaacggtatg cagagaggtt cgacgcatca gcgcctgcaa ttgagcgtag
    19921 gtctcgcctg tcggggcttt cactttaatc caatccgggc ggcggcgcgg cctggggtct
    19981 gaaggagcgg gctggatacg gtcacgggtg ggtgtgttgg ccatggtggt ctccagaacg
    20041 aattatacct cgcatgaagt gtggtgctga tgataaaatg cattataagc ggtacggccg
    20101 gaggaacggg gtttgcgtga tatactgccc ctatcagtga atcaagcatg ttgatcttag
    20161 ttgccacacc catcggtaac ctgggagata tctcgcaacg cgcgttggcg gcgcttgaaa
    20221 acgcggacgt ggtggtgagt gaagataccc gtaagactgg tttcctctta aaccatttcg
    20281 gcattcagaa acctcaaatc tctttcagag aagataatga gcagcgcgcg ctgccaaagg
    20341 tgatggagtt cctggaattg gggaaaaccg tggccctggt caccgacgcc ggcacgccct
    20401 ctatttctga ccccgggttc atcctggtgc gcagcgcgat tgaaaaagca atcgaggtct
    20461 cggcggtgcc tggtccaacc gcgcttatta tggcgttggt gctgtccggt ctgccggtgc
    20521 acagcttcac tttcagggga ttcccgccgc gcaagagcgc tgccaggcag cgctggctgg
    20581 aaattgactg cgactctccg cacacgctga tcttttatga aagcccccac cgcctgctgg
    20641 cattcctgca agacgcgcag gcggtgtacg gcaatcgcag gtgcgcagtg gcgaacgacc
    20701 tgaccaaaaa attcgagacc gtctaccgag gaaccctcaa agaggtatca gagcagttag
    20761 cgcgggaaaa actgttgggc gagtatgttg tggtgattgc cggcgcggag gattaaccgt
    20821 tccagtcaaa gatgctgaac ctggtgtgcg tgtcgaagta atcttcctgt tcctgtcttt
    20881 ttaacgtgct gaccacccaa tacgtgaccg gcgtcatgag gacttcgatg gcggttttaa
    20941 aaaggtaatt ggtcaccgtc agcgtccaga aaaggctcca ggggaagacg ccggtgagag
    21001 tggcgatact gacaaaaacc agcgaatcca ctgcttcgcc caccagggtg ctgccgatgg
    21061 tgcgcgccca caaccatttt cctctggtgg cgaccttcat ttttgccagc acaatggaat
    21121 tcatgaattc tcccgccagg taggcgagga ggctggcgag cacgatcccg ccggaggtga
    21181 gcccgcccag tatggcgtta taagcgccct gaccgctttg ctccacccag gtggcttcac
    21241 caggcatcac gcgcactagc cagaacaccg cggcggtgag cgccaggcag ccgaacccgg
    21301 tccagatgac acggcgggag cgtttaaagc cgtacacttc ggttagaaca tcgccgaaga
    21361 tgtaactgat ggggaaaaga attgtgccag cgtcaaaggc aagcggtaga ttaaagatgg
    21421 acactcccca atcgacaatt ttcgccgatg aagcgatgtt gctgatgatg agcacggtga
    21481 caaaaagcgc catcaccagg tcaaaataac ggtaggaacg ctgaggggtt ttatccatgc
    21541 tgattcagaa acacgaaggg taacctcacg cctttagtat aatccagtac atacataagc
    21601 ggagagagga atgcagcgct tttttgtgaa cccagaggaa atccaggggc aggaagtggt
    21661 ttttgcgcct gcgcgctcca ggcagatcgc gcgtgtattg cggttgaaaa atggccagaa
    21721 ttgcatggtg ttggataacc gtgggggcga gctgctggtg gaattgacgc aggtggaagc
    21781 gggtgcctgc cgggcggtaa ttgtagagcg ggctgaagcg caacaggaac ggattgacct
    21841 gttgatgctg ttaagcctga cccagcgcga gaaattcgaa tggatgctgc agaagtgcac
    21901 cgaggtgggg gtttcgacgt ttcagccggt gatcaccagc cgttcgttgg tgcagcaacc
    21961 cgtacagaca attgccaagt atgaacgctg gaagaccatc atttgggaag ccgcagaaca
    22021 aagcgccagg agccgcctgc cagaattgct gccgcctgcc gggctggctg aggctgttgc
    22081 ggaagcggct cgacgttgcc agcgctgcct gatcccctgg gagggcgaga aggcacggac
    22141 actcaagggg gcattggctg gcccagcagt tggcagcgtt gcgctgttca ttggccccga
    22201 aggcgggttc gcggaaggag aagtacagca ggcggtaaat gctggtttca acgtggtgac
    22261 actgggcagg cgtattttac gcatggaaac tgccgcggtg gtcagcgcgg cgctggtatt
    22321 gcatgaactg gaatctacag aagcagatga cgggggaaca ggttcttcag ggccgattgg
    22381 gtgaccttcc cgcagcccag tgacagtccc tcctggttca aaataatacg aaagcggtca
    22441 gtttcttttg ccgggctgcc acgtggtaaa aggtccgcgc cagaaagcca caaccggctt
    22501 tcaatctcat cgagttgcac gatgttccgc tggcaccgag caccaaaacg gctggcccac
    22561 tggtgagaag gcaagaaacc gctggctgtt tgctgcccga gcagcatccc ggcggattgc
    22621 aggggtagag cagggaactt ctccagcaag agcatgggta ataaatgcat ttcttcgaag
    22681 tggcgaacca aagtacgctg atgactgctg atatccgcga gcaagtcata agcgtaagtg
    22741 ttatgaaatt cctcgcaaaa ggctcgctgc tcacgggcgg ttaattcttc aaaacccacc
    22801 tgggcgagcg gacgagatgg cggtgaggtt gggcgcatcg gcgtatgctc tattttacgc
    22861 aagtgacagg cgaagaaccc ggcagtgtga tagcggtggg gccacaggcg gatggctttg
    22921 ctcaaagcgg ggtgcagttc ggcaccatcc agcgaggtaa tggcaggcgc gggcgcgggc
    22981 aatttggttt gcgcgtcaag tatctccacg gcagaaccga agacgcgtag taattcctct
    23041 actaccatct catcttcctg cggcaggagc gtgcaggtgg aatagaccag ttctccgccc
    23101 acgcgcaggg tgcgcagggc gctggtaagc agggctaact ggcgacgaga aagctggcgg
    23161 ctttcttttt ctgtcacggg tcgggaaatg tgtgattctg cagttcgcaa cccctgcatg
    23221 ctgcaggggg catcaaggag taccttgtcg aacatgctcg ggaaccactc gccaaagcgc
    23281 tctccaggga aacgactgac cgccgcattc accgcacccc attgttgcag cacaaggcgc
    23341 agcgcctgaa tgcgcccctg gctggagtcg ttggcgatca ccaaccccga atcttccagc
    23401 cggctgatga gatgcgtggt ctttcccccc ggagaagctg ccaggtcgag gaccagctct
    23461 tcacgctgcg gggaaaagtt gaacaactca ggcggcagca tggaggctgc ttcctgaata
    23521 tacatcaagc ccaggcggtg ttcaagcaca gcgctgactt ctggcccagc cccgatggta
    23581 acgcggtaac ccgcggggca gaagggaacg gcttccaact gccagccgta tttttcactc
    23641 aggcgcgatg ccaggttggg cggggccttg agcgggttca aacggatggc tgtcggcaaa
    23701 ggctggttga tttcattcac cagcagctta aattcttcct ccgtcagcag gggtttgtaa
    23761 gcagccgcca tttcaccgct atcagcgggc ggaactgtgg gtcgctggcg cctgttaggt
    23821 ttttggctca tgcgtccgcc ctgatgcaaa agacgatttc gctgtcaaag agcagttcgc
    23881 tcacctgcca acctcgctgc gcgcaggctt cggccaccag ccggcggccg cttgttagca
    23941 gcgaacgctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gctgcgcgca
    24061 ggcttcggcc accagccggc ggccgcttgt tagcagcgaa cgctgtccat tgagcgaaac
    24121 ggtgggcaaa gagaccagca gccagcgcgt tcgtagccga tgaaaaaact ggcggttcac
    24181 ccctcgttct cgctgttcaa atcggtgggc ttctttgaag aaaaagacca catccgcctc
    24241 catctccggg ggttctacca ggatgtctcg gtgataggcg ctgccagttt gaccgatatg
    24301 ttggaagaag gcgtcgatca agtcaacgcg aggctgatgc aggtcgtagg cgtaataagc
    24361 tgtttcgcgg ggcagtccca tccaggggag cgcgaaaggg ttcaaaccgc aggccaggtc
    24421 cagcaccacg cgtggagtgc cggtgaacct gaagatctcc tggtaaaaaa gcgatagcat
    24481 gggcagcctc tcgcgggtgg aggcgtgggc tttcaacagt tgagcgcacc attccattac
    24541 ctcagcagcg tgcggttgat ttggcagagc cgccagcgcc tggcgcatca ttgtgtaatc
    24601 agggtcaccc aggtacgtcg ccaccaggtt gtgaagtttt tggcgaaata ctttttccac
    24661 ctggcggggg tcggaacagg cgcgcaaggt tttttcaagc agatctcgca gcgtagtatc
    24721 aggtattccc agcgcgcgat acttacgtga agcgctgacg cgcgcgagca gcgctgcata
    24781 ttgatcttcg gtgggagaag cggagatttt cttcatggcg cgcgggtcgt cacctctgga
    24841 ttaaaaacgc gagttcgccc gggaactcaa agcgggtaac ctgccacgct ttgccggcga
    24901 gtagctctgc aaagcgccgc tcgtagttgc gcaccatgcc cttggatttt cctcccaggc
    24961 tgtgggcggg gaatgaaacg agaatattgg gagatgagat catctccaga agacgccgac
    25021 cggcatcctt atcgatctgt tccaggcagg ggatggtctt cagcaccaga gtgaggtcag
    25081 ccttgacagg aggtaactcg cgggtaatat cgcacggtac tgcatggcca tcaatcttaa
    25141 acctggcgaa aaaagcgttg agaaaatcaa gcatatccgt gtagatatca caggcggtgt
    25201 aagtgaagcc aggctgcagc ggcagccagg gaatgaccag cgggtttaac ccgcaagcga
    25261 ggtccaaaat ggaatggacg ctgccgagag gagccaggca ctcctggaaa aaaacggcct
    25321 ggatgggtat ccgttcgcgg gtagacgcgt gagccggcaa ggcgtgcata aggtaactca
    25381 gcacatccgg gtgatggagg tcttgtggaa gctgcgcgag ctcttttaac caggctgcat
    25441 aagggatggg ttttccctgg taggcggcgg ccacctggtg gagtttactg cgcgcggctt
    25501 tgactgcttc ttttgggccg cgcctttttt ccatttcggc agtcagcacg gagcgtacca
    25561 aaacctcaga gagatggcga tacttttcag cccgctgcac ctgctctaca agggcgtcaa
    25621 gcagggtatc cggctcagcc atgcgcagcc tgttcccgga tacgttcagt gagctgcacc
    25681 ataaagcgca ggttgtgcaa ggtgcacagg cgcataaaaa gcggatcacc ctgtttaaag
    25741 agatggtaga gatacccgag cgggtagtcg cggcagcagg ggcaatcaca ccactggctg
    25801 atgggctggc tggagcggat gctgcgcggg ttatccacgt actggtaggc gaaccaatct
    25861 ccggtgagcc ctggcgcgcc gggcgatggc gcgctggtga aggtgtacaa cctgccgtga
    25921 cgggcgtcgc gggtgggcat ggcactgtcg aacaggtcgt aaccgaggtg ataagtggca
    25981 agtaaattct gtggatgccc gatgccaagg gcgtgcatgg ggaaagcggc ggggatgagt
    26041 tggcgagtgt aagcgatgat ctcggtgagc agccggtttt caccatctaa aggccatccg
    26101 ccaaaaccaa agccatcgaa gcctatctcc agcaaggcat ccgcgcagcg tttgcgttgc
    26161 tctttaaaac cgccgccctg caccacggcg aagagcagcg gtcgctgctg ccccgtttgt
    26221 tctttttgct ctgccagcct gagatacgct gccttgctga gacgcgccca ggcgatggtg
    26281 cgttccaccg aaaggcgctg ggcagcttcg tcttcatcca catgcgtgca atcatccagg
    26341 cagaaaacaa tatccgcgcc gtagctcatt tgcaactgga tggccttttg cggggtgagc
    26401 tgaaatttgc ggctggagcc ttcaggataa aataaaatgc ctttgtcgtt aatgctgccg
    26461 tagcgggcgt tttgacggat gagcgaataa gcctggaacc cgcccgagtc ggtgataatc
    26521 ggccggtgcc aaccagtcat ttggtgtaga ccgccgagcg cctggatggt ggaagacccc
    26581 ggtttttggg tgaggtgaaa cgtgttcatt accagcgctt caataccgat acctgccaaa
    26641 tcacgggcat ccaccgtgcg cacaacgccg taagtggcat cgggcaggaa aactggaaag
    26701 tgaaccttac cgctgacgag ttcgaggttg gcggctctca ctgtttactc gacctcaggc
    26761 gtctggtgtt cggacgagga ttttgtcttc acaaaatgat tataaggtat gaacgcggca
    26821 aatcaggctt ttctcgtttc aggcaccttg agcaaagcgc ctgcggagag gaacatcagc
    26881 agggcggaaa tcgcgaacag cgcggtccaa ccccaccact gaccaggagc agcgttattg
    26941 agggcgtcgg tcatgggtcc ttccaacctg gcgatggccg cggaaccagc ggtggcgatg
    27001 ttggtgagac ccatgtactt ggcggcgtta tccttgggga ccatgtcgtt ggcaatcgcc
    27061 cagttcacac tgagaaagat acccagcccg acacccataa aaccgccgta gatgagcaat
    27121 tgggcaggcg ttttggcgaa aatgagcaga atggcgccga taccgccgat gatggatgaa
    27181 aggatttgta gttttttacg accgtaacgg tcgctgaggg tgcccgccaa caggctgaag
    27241 atgatgagga caacgacgaa agtacccatg acgaactggg tgaacatgat gggatcctgc
    27301 tcagggaact tatcgcggat gaaatactgg gcgaaggatt ggaaaccgta gatacccacc
    27361 aggtagagaa aacggctgag gatgagctgc cagtaatgct tatctccaac gaccttgaag
    27421 ttgaaggttt ctttgatcgc cctgcccagc tgcaggtggt tcctgccttc accgatggag
    27481 ggactttctt tagtgaaaac caaggtgatg agaccaaata ccaccagtaa ggcgatgatg
    27541 actgtgaccg cgagccgcgg gtttgggtcg gaggaggaga tgagaaaacc catcaacagg
    27601 gaggagagca caatgcctgc catatccagt gcggtcttga tgccgctggc aagcccgagc
    27661 ttttcagcag gaacctcatc cggcagcaac ccctgagcgg gtccatgagc gatattggag
    27721 gtggtttgca gaccgatgta gccgatgaac agcgcgggca gaccgccgat aaaacccatg
    27781 agcagcaaaa aaaacaggtc tcctgctgtg ccaaaccaca tcagcgggcg gcgccgtccg
    27841 agtttcgatc gccaatggtc gctgaccgcg ccagagacgg gttggacaag catggcgatg
    27901 atcaagccga agaaggtgag cagcccaagc caggtgttct tctgggtatc tggcacgtat
    27961 ttaaggagca cggctggcag gatggtaacg tgcaggctgt tccacatgaa ggaaagacca
    28021 atccaatagg cgttgagcac aaaaagatac cagccattca ctcgtttcat cgttttctcc
    28081 tgatagaagg gcatctgttt atcgggggct tactccggca tggtcttgaa caccttgtcc
    28141 cacaacggcg agctgacgcc aaaccgcgcg ttgggcgttt tgtaatggtg ctgcatatgg
    28201 taacgcttga tgtatttggc gtacccgccg cgcatgggaa agtggtgcgt ggcgtaatgg
    28261 gtgaggtcat aaaggaggta accgaaaatg aaagcagaaa acaccggagc cacccactgc
    28321 gccgctttga ggatgaccgc gaaaaagagg taaaaaagag cgtagaaaat ggccgccagg
    28381 gggatggaga tcacaggcgg cattaccagg cgggttttta actgtggttg ggcgtgatgc
    28441 acgccatgga agagaaagat gatgcgttcg gctttttcgc ctttgaccgg catatgaaag
    28501 agaaagcggt gcaacacata ctcggcgaga gtccacagga agagaccgac cagaaaacca
    28561 agcggaataa ccaccggaaa accgccagca ggggcgtggg cgatcgacca gatgaggaag
    28621 aacagggcaa ctggcagcca gatgaccagc acggtaatgg ggctgatatg agagaagaac
    28681 tcgagaaaat cagatttgaa caggcggatg ggaacatcac tgtggtcggt tggcaggtgc
    28741 gccatttttt cctccatata aataaaccag gtagaggaat tatatacagg attgaccaaa
    28801 caagggggtg aaaaacggtt tgattgggca ggaatgaaaa agagcaggat ggtgtaaata
    28861 atcctcctgc tcctggtgaa accgataaaa caggttaatc ctttggcgga taaccggtga
    28921 tagcacgtat ctcgtcatac agaggtttca ggtggctgta catacgcagg taaacccgcc
    28981 ggtaaagctc gttatagatg gcgtaatttt cttgtttcgg ctcgaaggtc tcgcctttgt
    29041 gggtcatttc tttcaccgcg ctggagaaat cagagtgcag tttaagcccc acggcggcgt
    29101 caatggcggc tcccagcccg cttgtttcga acacatgcgg gcggctggcg ggcagcccga
    29161 acacatccgc ggtgagctgc atggctgcct tgctctggct gcctccgccg ctgacgcgga
    29221 cttcctcaat cggcacatga gtgcgtttac tggtccgttc agccccttcg cgtaaggcat
    29281 aggccaatcc ttcaaggatg gagcggtaaa agtgcgcgcg ggtatgcaca tctccaaaac
    29341 caatgacggc gcctttggct tcaggcccgg gtactttgag accaggtgac cagtaaggct
    29401 gcagggtcag ccccagcgac ccgggcggca ccgcgtttac caggtcatcg aacagttctt
    29461 cggcggctac gccgcgctgg gccgccagcc gttcttcgtt taaaccgaat tcgcgcttga
    29521 accatgagac catccagaag ccccgataaa tctggatttc gagcgaatat gaaccgggca
    29581 cggctgaagg gtaaggagga atgaggggga tgacttccac ataacggtcg agcgtggtgt
    29641 tgatggtggc ggtggtgccg aaggagaggc aaggaatggc gggattgaga caaccggcgc
    29701 caatgacctc gcaggctttg tcggcggcag cggcgatgag cagcagcccc tcgggtatac
    29761 cggtttctgc ggcggcttca gcggtgatca caccaatttg ctcggcgggt ttcaccaact
    29821 cgggcaacat ctccttgtta accggcactg cctgccactt ccaatcgttc ttgccgctcc
    29881 aatcttgctt tttgtaatcg aatggcaggt agccgacaag gcagccaaca gaatcggcga
    29941 accttccggt cagacgcata gtcagatagc ctgaaagcag caggtattta tacgtgtttg
    30001 accaaatttc cggctggtgt ttttgcaacc agttgccttc tgcctcagcc tgcaggtagg
    30061 ccacggtttc gctcatcccg gcgattttaa acagcaaccc ccaaagacca ccgacgggtt
    30121 tgaggtcgga ggtgcggcgc tggtcgagcc aatgaatggc aggtcgcagt ggtttccctt
    30181 ccttatcgac gttgatgagc gtggagcgct gcgtggtgag cgccacacca gcgatgtttt
    30241 cttttgccac cccgggttgc tgccacaaac tgttacaggc ttcacacaac gccttccaga
    30301 acacctgtgg gtcctgctct gccaggcccg gtgctggtga atagtaaggt tcaatgggca
    30361 cacggctgat agccaacagg ttgccgcgca ggtcaaagac catcgcccgg gtggattgtg
    30421 tgccgacgtc gattgccagt aaatgatcct tcaattcagc cctccaggtc cttgctccaa
    30481 cgctgctcct tctgttccgt ggtcatatcc aggccgagcg ttccgccggg attcatgata
    30541 ttgtgcgggt caaaatgagc ttttaaggcg cggatgatgt ccatctgcgc gctgccgatt
    30601 tgcccctcca gccagggagc ggtttgcttg cctacaccat gatgatggct gatggccgct
    30661 ccgctttgct gaatggcaga tagaatgccg tactgcaatt caagatactg ctttattgta
    30721 gcgattttgg cgataaagat aaagtacagg ttgccgcctt gcgggtaagc atgagaaaga
    30781 tgggtcatac agatagtatt ggggtggctc ttgataaaac cacgcacgct ggcatgcact
    30841 tctttcatct gcgaccaagt gacagcgcat tcgagcgtgt cagtgagcac accaaaatcc
    30901 tgcaggtctt cacgcatgta cgggtcgcga aaacggctct tttcccagtt actggtgaca
    30961 ggggcgaagc tgatgttgaa tgcgccatga cgccggcaaa tgcgcctgac ctggcggttg
    31021 acgttgcgcg aaaagcccgc ttcaccttcg gtcataccca gcagcaggca tttctgcatg
    31081 ggcaggtagc cgctgagcct gaggacagtg tcggccgggg tgccctcgat gtgatagagc
    31141 ttcatggcca catcggtttc ttctgggtca gagagcctga acaccgaagg attgccacac
    31201 tcgcactgca taatttcgcg gcaggcggct aaggcgtcgt cccaattctt gaacatgtaa
    31261 ctgaagcggt ggcggttgcc gggttgaagg cggaatacgc gcagggtcac ttcggtgagc
    31321 acgccaaacg cgccttcact gcccatcatt atttggtcaa aatcaggccc gttggcgctg
    31381 cgcggatgcg ggcaggtctt gagctcaccg cgaggggtgg cgtaacgctg ggatatgaca
    31441 atatcctcga tcttgccata ataggtggag ttttgcccgg ccccgcgggt cacgacccaa
    31501 cctcccaccg atgaaaattc aaaggactgg gggaagtgcc cgcaggtgta gcggcgctgc
    31561 gcgccaagcc gttcaggcgc gtgattgagg atttcttcca gctgcggacc ggtcatgccg
    31621 gtctcaacgg ttatggtctg gtcggtttca ttgaaagaaa ccacgcggtt catgtgggta
    31681 ctcatatcca gcgcgatacc gcccttgacc gcttcaactc cacgggtaac gctggagccg
    31741 gcgccgtaca cataaacagg gatttggtgc tgattgctgt annnnnnnnn nnnnnnnnnn
    31801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31861 nnnnnnnnnn nnnnnnnnnn ntcttcgggc gcaagatcga aggaactcat catcagtttg
    31921 accaacccgc tattcgggtg cttgaatgca gccgggtctc cccactttaa cagtgaacga
    31981 taagtgcctg gaggaacgag ttcttcgtac caattgggtt tgtgtttttt ttcttttgcc
    32041 atggttttgt cctcctagaa accagccggt gaagggctgt aggctttttt atacaattca
    32101 acatagcgcg tgaattcagc ctgccagcgc gcgtcgtccc agccaagctc tggctgcacg
    32161 atttcgcgca cgcgcgccat ttcctcctgg gcgccgtgcg gcagcagcag acccagccgc
    32221 acacggcgca ataataaatc gtccaggtgc tcaaccgcgc cgcagcgggc agcccaacgc
    32281 acttcagacc aaaggttggg caggctgcca acaggctgat tttcaccttc ttcggcgttt
    32341 tcaaatagtt gaatggtttc gttcccataa cgacccaaca gataaagcca ggtttcagca
    32401 gccattccct gtggagatga tcgagtgggc agcggggaga aataggattt tgggcgtgaa
    32461 aggttggccg ggtggggcaa cgcgggcatg gctgcgcgca gcgcgtcttc agccatgatg
    32521 cggaaagtgg tgtacttacc accggtgatg gtgaccaggc cgttctcttc ccaaaccaca
    32581 tgggcgcgag attcttttga cgggtcggct tgaccggtat tgaccacggg gcgcagaccg
    32641 gagaaggttg aaatgatatc agcccgatcg agttgaagcc caggaaaggt ggcgttggct
    32701 gcttccagga tgtagtcgat ctcttcctgt gtggtgtagg gttcaccctg tgagagcggc
    32761 agggtgtggt cgaggtcggt ggtgcctatg aggctcaccc cctcccaggg gatggcgaac
    32821 atggcgcggc ggtcgcgggg gtgcagcaat gtaaccgcgt aaggcaggtt cagacgttgg
    32881 cgggaaaaca ccaggtgcga accgcgcaat ttacgcaggc gcgcgggagc gttaacaccg
    32941 gcgcgaacct catcgctcca cgggccggtg gcgttgatga ccacgcgagc cagtacttct
    33001 ttctcctgga aaccgcccgt ggctgtgtct ttaattactg ctccgcagac cctgccattc
    33061 gtctctttga gcagcgctgc tgctcgcgcg taattaatgg cgcagccgcc agcgctgacg
    33121 gattcacgaa tcaggcgcaa gaccatgcat gcgtcatcca tgccggcgtc gtaatagaga
    33181 aagccacagt gaagttgatc ggcatccagc gccgggcatc ggtcgagcat gtcatgacta
    33241 ttcagacggc tatgatgcca ttttggagcc aacaggtcat agagaataac tcccacggcg
    33301 aattgcgacg ttaacttgcg gtcgtccgga cagggcatta ggaaacccaa gggcgtcacc
    33361 aggtgccgcg cttctttgag cagccattcc ctttcccgca ctgactcacg tgtgacacga
    33421 aattgcttat tgcgcaggta gcgaaaccct ccatggataa gtttggatga cctgctggag
    33481 gtgccgctgg aaaaatcgcc cgactctacc agcagtgtac ggtaaccctc ctgcaccgcg
    33541 cgccgcagaa ttccagcgcc ggtgatgcct ccaccgatga tgaggatgtc ccatttacca
    33601 tctaactctt gccaggcgcg ctggcgccaa cctttttccc acataaattc cctcctctaa
    33661 ctttacgagc ttgtttgccc ttcaatctct tgccaaagtt caaggctttc gcgcatcacg
    33721 gcgcgtgtga cctcttcagc catgaaaata tcattggaaa gcgctgccga gagcagcttc
    33781 ttataatacg atcgggagtg atcgcgggtt aacggggaga gaaaataacg cgaccccatg
    33841 ctgtggtaca ggtctgtaaa accattcagg ataagcgtaa aaatggggtt acccgaaaga
    33901 atggtcaaac ggcagtgaag ctcgaggtca acccgggtaa agaccggagc cgcgtcaggc
    33961 agggtctgca ttgattccag caggcggatg acctcggagg aggcgttgcg aatggccagg
    34021 cgcgtgtatt caggcgcgag cagggcgcgc acatgcagca ggttgggcac gaaatcagca
    34081 gggagatgct gctgatggcg ggcgatagcc gaaaggatga gcaggttgcc ttcctgccag
    34141 taatcacgta cgcgcgtggg acgtccctgg tggatttcta tccaaccatc gcgtgaaaga
    34201 cgctgcaaag cctcgcgcag cgtagggcgg gttacaccca gcgacagcgc cagttcacgc
    34261 tcaggaggga ggggtgaatt gaccgggaaa ttgccatcca gaatggcgga tatcagccgg
    34321 ctttcggtca gttcagcggg tttttgcgga gtggaccatg cgatcatcgc tcaccttggg
    34381 cagagggaat gaatggtaag aggtcatacc aatcaatttc attatagcgg tttatgggtt
    34441 tttttcaagc ccgaatttca ttttatccac agatatacac gcaatttacc aaagatattg
    34501 gggtagccga ccaggagacc cccgcatttt gctattgaat aaatagctgg aataagaatt
    34561 gcaatacaaa aggctctata atccaaagag ggcatttccc cgcagcttgc tgcgaaaaat
    34621 agtaagatta aaggatgagt gagacttgga gaaagagtca caacatatcg attttgattt
    34681 accactttgt atgtccagca aagtatcgga aagtagtgtt caacgacgaa gtagatgaag
    34741 tgttgaaaaa cacgtgttta gaaattgaga aaaggtatga aattaagttt attgaaatag
    34801 ggactgataa agaccatgta cattttctaa tccaatcagt accgatgtat agtgcaaaga
    34861 agataataca agtagtaaaa agtataacag cacgagaagt atttgcccaa gtaccagagg
    34921 tgaagaaaaa attatggggc ggagagtttt ggacagatgg ttattatgta agtacagttg
    34981 ggcaaaaagg aagtgaagaa acgatcagaa agtatgtaca agagcaagga attgaaaagg
    35041 actacaagca tttgcattat gacgaacagc ttaaattgtt ttagcaagga aaaaatctaa
    35101 agacgatacc ccgcagcttg ctgcggggag gattcattta atccataccc ttccaggaga
    35161 aacccgatgg caaaaaaagt cgtgaacccc actgaataca acccggtcga ccgcagtgaa
    35221 cccatgagcg aagccaggcg cgcggtg
//
LOCUS       DAOSPM010000005        34285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1292_length_34285_cov_13.383260, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000005 DAOSPM010000000
VERSION     DAOSPM010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 34285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34285
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1292_length_34285_cov_13.383260"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..679
                     /locus_tag="PLW25_01160"
     CDS             314..679
                     /locus_tag="PLW25_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53127.1"
                     /translation="MSADGISSLAKGCCATPEWTGERFFVLFPGGSLTDDTKDKLRRY
                     NVNFREAKKNMKQVIASADVTLVEPMVRTDHPKHGEEEGERGKTSAVVALTDGWFINK
                     SPLRCHSASLKIAWKWNLP"
     gene            801..1781
                     /locus_tag="PLW25_01165"
     CDS             801..1781
                     /locus_tag="PLW25_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOS53128.1"
                     /translation="MHTFLHGRDLIGDLDFSKEEVETVLDVAFDLKRKRALNELTPYL
                     RDKVLAMLFFFSSTRTRGSFEAGMAHLGGHAAFIESRTTQISHGDTPKEIGEIYGRYF
                     DGISIRHCDWGVGNGYLNEVAKASRVPVLNMQCDIYHPFQCLADLMTIMEKKGRDLRR
                     KKMVVSWAYAASYQKPMSVPQSLILQMPRFGMDVVLAHPPEFRLMPEIMEQAREQARK
                     FGVGFDVVEDMDEAFKGGDIIYAKSWGAMVHTPDPEEGAKIIKKYESWITDERRMKLA
                     KPDAIYMHPLPADRNIEVTDAVLDGPQSVVYDEAENRMHVQEAVMALTMG"
     gene            1834..2790
                     /gene="arcC"
                     /locus_tag="PLW25_01170"
     CDS             1834..2790
                     /gene="arcC"
                     /locus_tag="PLW25_01170"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOS53129.1"
                     /translation="MTKKLAVIAVGGNSLILDPKKVTIEDQYTAAAETTKHIADMIEA
                     GWDVAIGHGNGPQVGFLLRRSEIAAKYEGMHEVPLDSCGANSQGAIGYWLQQTLQNEL
                     ARRGIKKPVATVVTQVKVDKNDKAFQNPTKPIGGFMDEAEAKKREQELGWNVVEDSGR
                     GWRRVVASPLPVEVVEIDSVRALLNAGVVVITVGGGGIPVVDNGKGGCVGTAAVIDKD
                     YASSLLSTLINADLFIISTAVEKIAINFGKPDQKWLDRMTLAEAKAYLAEGVHFAKGS
                     MAPKIQAIINFLEAGGKEALVTNPENIGRALRGETGTRIVRE"
     gene            3070..4347
                     /locus_tag="PLW25_01175"
     CDS             3070..4347
                     /locus_tag="PLW25_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOS53130.1"
                     /translation="MFTKKLWLSLSVLLVAVLVLSACAAPAPTEAPAPEVPAATEAPA
                     ATEAPAATEAPAKAPVIGLLLIGPANDAGWSQAHYESMAYVKDKIPGTELLYLENVYT
                     GSTSHTQTPAELAADMVTKGANIIIFNSDDQKDNAIKFAQEHPDVFVIHPSGDAAWKE
                     GKNYANLPNMKNIMGEMEYGKMIAGCAAALTTQTGKIGFLGPLINEETRRFAASAYLG
                     AKYCWENYLQKDPADLEFKVTWIGFWFNIPGVTLDPTQVSDDFYNTGYDVVISSIDTT
                     EALVEAKRLAADGKKVWAVPYDYYGACEGAQEVCLGVEYFNWGFAYVDAIKSAMDGTW
                     KSSFELVPPDWKDINNPDTSGVGFIKGGGLSAENAAKVDQFIAELGAGLNLWKGPLNL
                     QDGTVYLADGEVATIQQIWYLPKLLEGMEGQSE"
     gene            4413..5876
                     /locus_tag="PLW25_01180"
     CDS             4413..5876
                     /locus_tag="PLW25_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS53131.1"
                     /translation="MKISFSHIHKRFGNVHANNDINLVIPSGSIQGILGENGAGKSTL
                     MKILTGYYHADSGEILLDDRVVKITSPVDAVRQGIGMLHQDPLDFPPMRVIDNFLLGS
                     RGGLFPKRSDVEKEILRLSREFDFQISPDTYVETMTVGERQQLEILRLLWLGVKVLIL
                     DEPTTGISANQREKLFATLVKLAKQGLTVIFVSHKLEEVEQLCDRVAVFRQGEVVGEV
                     EPPYDTDQFVYMMFGKSVEVGDKKTFCTELPLIQLENIGLEDYRLHLGNINLEIYQGE
                     VIGLAGMEGSGQRLFLHMLTGLLRPVNGKIKLTGEDFTGKTYPYFQKAGVAYLPAARL
                     EEGLIPGMTLTEHFILSEEHSELFVDQKSALGLTEERIRDFYIKGTPVSMIEQLSGGN
                     QQRALLALLRRKLKLILMEHPTRGLDIESAIWIWSKMKERCKECSSVVFTSSDLDEII
                     QYSDRILVFFGGLVSEPIDATTTTVDELGQLIGGKGW"
     gene            5899..6972
                     /locus_tag="PLW25_01185"
     CDS             5899..6972
                     /locus_tag="PLW25_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS53132.1"
                     /translation="MSVELRYSRSARSLKKSAWVNVGIRLSAIIMALLFTSVILLIAK
                     ANPLQAYKSILHGAFGSTIKLTSVIASWVPLVIATSGLLFTFTAGLWNIGMEGQIIMG
                     SVFAVGAFRFLDDTSLAPALVITLAFVAGAVGGILWALLPGLMKIFGGVNEIFGGLGL
                     NFVAKTVAIWLIFGPWKRPGVASGSGTEWIDIQYRLPYLPGTNLSLTILIIAVIIAII
                     TAVMLKGTHFGLRLKAIGKNDKAAYILGVPTSRYFLLAFGICGFFAGIAGSILVTSKQ
                     YSLIPDAGFGYGFLGLLVAMLVNYHPIWSSVVALFFAALSIGNTELQYMGLNSYFAGV
                     LQGFLVLFVVLVDGVRKQLVKTR"
     gene            6983..7975
                     /locus_tag="PLW25_01190"
     CDS             6983..7975
                     /locus_tag="PLW25_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS53133.1"
                     /translation="MEMTTLTTLAGIVEFAVPVLFAAIGENITEKAGLINLSVNGTII
                     LSAMGGFVVALTTSSILLGFLVGAVIGAAVAAIVAYSSITLKQSQVAIGFVLAMLCRD
                     LAYFLGAPFTNKPGIFLSKVPIPYLVDIPIIGPIFFNHSLVVYASYLFIIIAYVYVFK
                     TRPGLMLQGIGEKPAAAFARGANVKGLRYLYTITGGALIGLAGPMYSLAVKIGWAGQL
                     SGLDGIGWIALAITIFGGWNPLRVAIGAYLFGGLQQLGITLQSATNIPIQILQAAPFP
                     LMIFTLLLVNVGRADWVDRELAAMPERPRKVLSKILHALRTSPPSAIGVPFENE"
     gene            7985..9247
                     /locus_tag="PLW25_01195"
     CDS             7985..9247
                     /locus_tag="PLW25_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOS53134.1"
                     /translation="MNHYLGYRCTFCDTEISPTEILYTCPACGGNLDVVLDYRRIQQK
                     YRPEDIFSRAEGSLWRYEPLLPVTDPGGLATPLHAAGWTPVYQPERLIRGLGLRKFWL
                     KDEGKNPTASFKDRASAIVVARSREIGADVVVTASTGNAGAALAGMAAAIEQNAVILA
                     PKTAPAAKIAQLLIYGAQVVLVDGSYDDAFDLTIQASREFGWYCRNTGYNPFTAEGKK
                     TAAFEIWEQVLRHFRAGNKPLCVFVSVGDGNIISGLHKGFKDLMALGWLERMPRLFGV
                     QSELSSAVADAYLAGTEVITPVKATTLADSISVNLPRDGGRAVRAAVETEGAYIKVAD
                     ADIIRAIIDLGAAGIFAEPAGAAALAGLQKALAEGDLLEDDPVLVINSGNGLKDIRAA
                     MMAVPNPAIIEPTMSALKLHLEKKGRIE"
     gene            9244..10188
                     /locus_tag="PLW25_01200"
     CDS             9244..10188
                     /locus_tag="PLW25_01200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS53135.1"
                     /translation="MKPVFSIIAPVYNEIACLDTLLDRIQKVMDQTGEPWELVLVDDG
                     STDGSTDKIRQLAENDQHVRKVILARNFGHQIAVTAGMDYSRGDAVVIIDADLQDPPE
                     VILDLIARWREGYQVVYAQRTEREGETWFKRVTASVFYRSIYRITDIKIPLDTGDFRL
                     LDRKVVNVMNSMRERHRFLRGMSAWVGFKSIGVPYKRAARFAGSTKYPFKKMLKLALT
                     AVTSFSYVPLQLAMWLGFISAGISIITIPVVIVLRLVGSQFFFGQATTLIAVLFLGGV
                     QLISLGILGEYVGRIYDEVKGRPLYTTSETPDDALKTS"
     gene            10205..11671
                     /locus_tag="PLW25_01205"
     CDS             10205..11671
                     /locus_tag="PLW25_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOS53136.1"
                     /translation="MAKIDLQIFEERRQRAIERCRKQNILFPTFAQMKNPDLIPQKIK
                     EELSSIGLWDIHPRNLFRITWKNEPLEKGGGYGGANYLELPTELTGVKARIIALVGKW
                     FPTGAHKVGAAYGCLVPRLVTGQFDPTLQKAVWPSTGNYCRGGAYDSALMACQSIAIL
                     PEGMSRERFEWLNRVAGEVIATPGTESNVKEIFDKCWELRRSGEDLMIFNQFDEFGNY
                     LWHYEVTGHAMEEVLKKEMRPGDRYRGVALTTGSAGTIASGDYLKQLFPESKIIASEA
                     LQCPTLLENGFGAHRIEGIGDKHVPWIHNIKNTDLVVAIDDNAVMEIMRVFNEPEGQK
                     YLASLGLAEALVSKLHLLGFSGIANLLSAIKFAKYYELGEEDIVLTVLTDSMELYESR
                     VVEIQKEKGKLTQLQAAAAFSRYLLGERTDNLIELSYQDRRRVHNLKYYTWVEQQGKT
                     YEEILDQWYDPTYWTDVQGQAEEIDELIIDFNQRTGLL"
     gene            11731..12312
                     /locus_tag="PLW25_01210"
     CDS             11731..12312
                     /locus_tag="PLW25_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53137.1"
                     /translation="MQRSISPNALLLLGILPSPRDLEIARVLGWYRVPLKSSPKVIEV
                     DYLAFYQGANFGEQHRWKVEFFAEYHGHELTTRAELLRDQPDHPRANEEYFKIQLGPI
                     LPVEPPIVSAGWKRITFLFTTGELFNQAETINDLVVRTEERAVLWKKLRERNREMNRY
                     RAGASPNAEIAPEVLDLLLGLNSNLPDTDLENY"
     gene            12378..13799
                     /gene="hydA"
                     /locus_tag="PLW25_01215"
     CDS             12378..13799
                     /gene="hydA"
                     /locus_tag="PLW25_01215"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOS53138.1"
                     /translation="MQTLIKNGVLATATKTEEADLLIEDDKIISVGQGLTVEDAVEVI
                     DARGLYVLPGGVDVHVHLDLPMFNTVSSDDHYTGTKAAACGGTTTVIDFVSQETDDLL
                     GCVAKLRQKADARVAVDYGLHMNITHLNPRVEEQIPLLVKEGVTSLKVFTAYNGRLRL
                     QDHEIFRVLRIAGEHGLLTMLHAENGDLIELLVQEACLNDRKSPVWHARTRPAWGAVE
                     AVMRGAALAAVAQAPLYIVHINTSGEVDMLRYARDHGVQLMGETCPQYLFFTEDDLAR
                     PDGAGWVCSPPLRTAADQQRIWKGLKEKTVQVISTDHCPFFLNGTKPILYEGKEVAIP
                     GKELGRDDFTMIPNGLPGVGDRLPVLWTKMVGSGEFSINEFVALTSTNPARIFGMYPR
                     KGSLSPGADADIVLWDPRLEVVYGVAHAQQRTDYNLYEGWKLTGYPLRVLSRGRTVYC
                     DGTWSGKPGNGKFIHRAAFTGMV"
     gene            13808..>14953
                     /locus_tag="PLW25_01220"
     CDS             13808..>14953
                     /locus_tag="PLW25_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53139.1"
                     /translation="MLSFLPVILLLLSAAAIQVVRRMTKRAGSTWLMAMIFGSVTWLS
                     MILVGVLLPPAVKIDNWLSLPHQFTAITFQLTRETWVLGFLLVSLLQAIILFNSRYID
                     TPNFLNKITSSMLLTAFGLMAVLARTPLAFVLTWSLMDLAEFGVLTITLDQTLQTRSS
                     FAAVLLRELGIILLVLLMAVSPEQNLHPGDTTPTLIAWLLGLIFLLRLGILPILPDQE
                     DDSRLRRGFRTLLRTLPFVTAIAFASIFPGLTLAEKGSQIALPALSFAGLLAAILWFF
                     SPDELSGRPYWFFANGLLGLMAFLVNRVEALPGIAVIAVSAGSGLFLYSPKTLKRLAF
                     LPFLLVGLLALPFTPTVGILSFFTLGIFRFNQVLAVTTYALLLAGVLK"
     assembly_gap    14954..15053
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15054..15421
                     /locus_tag="PLW25_01225"
     CDS             <15054..15421
                     /locus_tag="PLW25_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53140.1"
                     /translation="QHIEANTIKSGAGRIFYSVSFSFCILLVFFTIQKTTRFLAIGQD
                     QSMPALLVLLCGFILLGIAQHPFRIIMGLLTIITGFEVLYSAVEQSLLVNGLLGAVVL
                     LIAWVGSYLLMPTTPGEEQ"
     gene            15418..16812
                     /locus_tag="PLW25_01230"
     CDS             15418..16812
                     /locus_tag="PLW25_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOS53141.1"
                     /translation="MSAPVIWIVFPLVVALGQLFLLKYPTILKLTGIVVSLLLALIAF
                     FQPIGNVLRLGGMVLDIRPDLQFLGRALHLDNGDRFILAGLFGILFIYTFLWNTHTVP
                     AKFAPLSLAISAILVAALAVQPFLYAAVLVELAVLLMIVLVKDNQMNQEKGVIRFLVY
                     LTLAMPFILFSGWILGGTQASPSEQGNLLGAAITLMIGFSLWLAVFPFHSWVPQFSRA
                     VQPYLSGFIISLFPIVVLFVAVNYISSLVWLRSASYLAPVLSSTGIIMIVTTGLWASV
                     ERDVKRVLGYCVLLETGFSLIMISFKSEQGLILMYHSLFPRVLSLALMVYSLSILHQA
                     GIALTHDGFQGVIRQYPFAASGFLISLFTISGFPLFAAFPIRLELLYQLGHISVPSVI
                     WTVFGLSGLIFAVIRLFRVMTLPAPNNWRIHESVTQVIIICTVILVLILAGLFPQLVE
                     GIIKPFTADLPLLK"
     gene            16842..18020
                     /locus_tag="PLW25_01235"
     CDS             16842..18020
                     /locus_tag="PLW25_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53142.1"
                     /translation="MENEEIVKRIEWLDGERRKDRQTIIELQAQVSKLQEVIALQKRE
                     LSNINKDNKKSFSSVVRLDEYEETNAKLKAELAKSQRLIEKRLDTMEQTTAAQRSDDR
                     DTYNKRMLELQGEIKALNELKKSVQSKGEDDFRLNQKLEDLARQLADLRSSGDEQNRL
                     AKILDDNYRVESKRLSDLQIELNTLRKRIDEDRNLSDAQKEYLKKLEGQIHDLVNREK
                     TRNQEQIEFIEKQSLLSVDRENLWKDWQEEIQQMKALSENFHGQVRELETLNREIKQG
                     QSDFEVINQRLERRINEITEMNRLTEERFRQEWIAFKADDQKRWTNYSLSQEETNREG
                     NREINKIDERITSLEDAVQNLLDTVQVIHEETEKRIKGLLMLANEYITSYERTALKKG
                     "
     gene            18024..19907
                     /gene="selB"
                     /locus_tag="PLW25_01240"
     CDS             18024..19907
                     /gene="selB"
                     /locus_tag="PLW25_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOS53143.1"
                     /translation="MRVIGTAGHVDHGKSTLVKALTGIDPDRLKEEKKRQMTIELGFA
                     WFESQSEGEIGIVDVPGHRDFIDTMLAGVGGIDAVLLVIAADEGIMPQTKEHLAIIEL
                     LRVNTGLVVLTKVDLVDDPAWLELVEMDIRERLSSTCLENAPLLRVSAVTGDGIAQLR
                     AAIEEVLAPLPPRLDLGRPRLPIDRIFSLTGFGTVVTGTLLNGSLCVDNEVEILPSRR
                     SARIRGLQTHKRSEETALPGSRTAVNLSGVDVADIKRGEVITLPRLLEPTQRIDAQIE
                     LLQDAPVSLKHNDLVKVFALTSECAGRVRVLQGQELKPGENGWVQIEFEKEMVLEKED
                     RFIIRRMSPAHTLGGGVVVNPHPGRRYRLQDSSVIERLSLNSGTALENRILALLMEKP
                     FITVDEIQNRLETDSERVREILAELTGRGQVSVLAGLTDQTTGYLPRAKWAELTAKSV
                     QLLQHYHQQNPLKSGINVDELARRLRISQDELNLCLREWLKEKVVKLQDGAIALPGFE
                     VRYSSNQSRRLEQFTAILDQQPFLPPGVKESKDLLTDDVFQSLVERGALVQLSTEVVV
                     RKAEYDLMLGYVIERCSRGELLTLAQFRDHFNTNRKVSQAFLEFLDRKGVTQRVGEGR
                     VLK"
     gene            complement(20331..20540)
                     /locus_tag="PLW25_01245"
     CDS             complement(20331..20540)
                     /locus_tag="PLW25_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53144.1"
                     /translation="MRVEGNPAGARTHLFTLRIWAVNEGTAAPKWRSRLQNIRSGEVF
                     FCENLSDLLSHIEETLCEQDQDEVP"
     gene            complement(20546..20908)
                     /locus_tag="PLW25_01250"
     CDS             complement(20546..20908)
                     /locus_tag="PLW25_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53145.1"
                     /translation="MKALRITLLVFTIYTAIIGILFLFAPRIAETAFQTRLPDAALTM
                     LYGQVVLVLALAAWLIRSDAAALRKMVWALVFAEVGHVVVFLWQLMNGISTFAQVGPP
                     MIIAAVFAVLFVVFNRKG"
     gene            complement(21133..24165)
                     /locus_tag="PLW25_01255"
     CDS             complement(21133..24165)
                     /locus_tag="PLW25_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS53146.1"
                     /translation="MALLLSYLALHADAALTRRQVAYLFWPETGDEQARTNLRNLIHY
                     FKKALPEADALLELDGQTIRWRDTSSLSVDALQFRAALAATRAVQDDETRIRHLQEAV
                     NRYRGELLPGHYQDWVLLIREEFQQAFLDALAQLTKRLEEVRRYEEAVEAANRLIRAD
                     ALNESAYLQSMRLRALMDDRAGALQVYYACATTIRRELGVEPSQEIQSFYQRLVGAEQ
                     NAQAQFREEKAPEQMRLVGRKQEWSRLKEAWGSVQKGKAALVLILGEAGIGKTRLASE
                     LMQWARRQGIRAARAECFAAEGNLPFAPIVAWLRTPEIREERAGLEALWKHELARLLP
                     EDAAGGTPSSGRKWERRRLFEAVARGLLGSGAPRLLMLDDAQWSDSETLEIVHYLMRY
                     DANAALMILMTARVEELDEEHPLTQLRLSLQERKQLHELELMPLDKPELGLLARDVLG
                     RDLEKAEEDELFRQTEGNPLFIVETLRSGKINETGMPQTLRSVLTRRLGGLSAAAREL
                     VGVAGVIGREFRYPLLARASQMNESVLVQALDELWLRRVIQIQRADAYNFTHGKLQEV
                     AYEALSAPRQQLLHRKIAESLEVEREDPAVIARHWDAGGQSAQAVEAYRRAAEAARRV
                     FANSDALAHLERALELLKEKIGAEMEEQKKSAGEICEALGDIYEMGNNQKSLERYREA
                     LTLVESSDGLSLARVLGKLARAEANHGNLQEGLNLFAQAEAALGEPSEAEDWQRAWLK
                     VHFDRVWVEYDANRVEALEAALAPIRPVVERLGELEKLGEYYFLFPTAYFRRDGYQIN
                     DEIMRYSKLALETSAKTDNLELQTRTNFGYGFCHFLRGDFDEAIHYLGEGLRLAEQIG
                     YVELQLMSVTYLAAAYRGAGRVEECQTCAERALTLSEREGFLAYVAAARANLGWAAWR
                     RSNLRQAKALSLSALEMWGAGYPFRWLALWTLIDLSLHSLRTDEALEYARKLKEPHQQ
                     IFEKEGDDLLIQASSADVGNAAPLLAQALAWAKKRGYL"
     gene            24599..24916
                     /gene="rplU"
                     /locus_tag="PLW25_01260"
     CDS             24599..24916
                     /gene="rplU"
                     /locus_tag="PLW25_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOS53147.1"
                     /translation="MKYAIVESGGKQYRAVEGTTIDVDLLDVEVGHEVKLAEVLLVVE
                     DEKVQVGTPSVKGAVVTTSVVDHIKGPKVDTFKYSPKKRIRVKSGHRQRYTRLKIESI
                     NLE"
     gene            24922..25185
                     /gene="rpmA"
                     /locus_tag="PLW25_01265"
     CDS             24922..25185
                     /gene="rpmA"
                     /locus_tag="PLW25_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOS53148.1"
                     /translation="MAHKKGGGSSRNGRDSNSQRLGVKKFAGEQVLSGNILVRQRGTR
                     IKPGLNVKVGTDDTLFAVAHGIVEYETLPSGQKQVHVRPVETK"
     gene            25254..25457
                     /gene="rpmE"
                     /locus_tag="PLW25_01270"
                     /pseudo
     CDS             25254..25457
                     /gene="rpmE"
                     /locus_tag="PLW25_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            25446..25730
                     /locus_tag="PLW25_01275"
     CDS             25446..25730
                     /locus_tag="PLW25_01275"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOS53149.1"
                     /translation="MQARQDYVEEKKSKLAAKTSPERSVKDIGITVKAAEALEKAGLM
                     NVGQLLEKLAEGEATILAIEGFGRKSLIDMKKNLRQMGYKLPAAAEEIVI"
     gene            25849..26421
                     /locus_tag="PLW25_01280"
     CDS             25849..26421
                     /locus_tag="PLW25_01280"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOS53150.1"
                     /translation="MDRKSGHIEVICGSMFCGKTDELIRRLRRATIAKQKVQVFKPAI
                     DGRYGLQKVTSHAGSEFGATPIRSSSEIIPLLKPETTVVAVDEAQFFDDELIEVVEEM
                     VTRGLRVVIAGLDMNFKGEPFGCMPALMARAEQVDKLQAICMVCGEPASRTQRLVDGK
                     PARYDDPVVIIGASEMYEARCRKHHEVPKD"
     gene            26438..27022
                     /gene="hpt"
                     /locus_tag="PLW25_01285"
     CDS             26438..27022
                     /gene="hpt"
                     /locus_tag="PLW25_01285"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOS53151.1"
                     /translation="MQDYHYYLSEVLITEDALQRRIAELGGQISRDYAGKGELVLVCI
                     LRGGVLFLTDLMRKVTIPHAIEFMAVSSYGVGGRESNGQVRITLDLNLDIFNKHVLLV
                     EDIIDSGHTISAVIDFLATRRPASISVCVLLDKEERREVEVPIRYRGFTIPNKFVFGY
                     GLDLDDYYRNLPFIGVVDLQKYQGSEQGSEGAIS"
     gene            27045..28367
                     /locus_tag="PLW25_01290"
     CDS             27045..28367
                     /locus_tag="PLW25_01290"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53152.1"
                     /translation="MTEPSKPLYLVGGAVRDALLGKPCSDFDFVCAQDPRPMARAFAN
                     RNHGNYFILDEGRLICRVLIDSAEVPDLIVDFAIMRGENILEDLAHRDFTINAMAIDL
                     NQPEHIIDPFKGGRDLLEKRLRVVSRDSFIEDPLRVIRAVRYTVDLGLTLAPEIIDLV
                     RAAVNGLQRVSLERKRDELFKIFSGHKVHTALQLCMLFGIIPYLPLSIPLNFPPLVTR
                     IRSLEDILDWLCGVKTHARQTAFYQSSLLVELGRFREDLRRFYLAKNPSKRDRRSLLF
                     LSVILGEQAEPGDHVRALALSVEEMKQVRDFKAAIPLLQGFIDRGSIPEPLEIYRYFK
                     ATAAGGVDAAVAALAEQVSHVGCEFVQEQWLYQLKLAARLMEAWFKSPQVLNPDPLLN
                     GDDLMRRFSLSPGPFIGELLELLKEQQIKGSVTSASEAWRWIEGELKK"
     gene            complement(28379..28792)
                     /locus_tag="PLW25_01295"
     CDS             complement(28379..28792)
                     /locus_tag="PLW25_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS53153.1"
                     /translation="MKILIIDDDPSVTDLLRLILAPTLAEIQHACDGEQGSGLVREFK
                     PDIVLLDYMLPGIDGLETTRQIRKITKAPILILSVLDDPLTLAKALDAGADEYLIKPV
                     SRGVLIAQINNLVRRYSEKSVDHSPLKVSWQTALQ"
     gene            29006..31786
                     /gene="ppdK"
                     /locus_tag="PLW25_01300"
     CDS             29006..31786
                     /gene="ppdK"
                     /locus_tag="PLW25_01300"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOS53154.1"
                     /translation="MTDKKWVYLFNQIKEVEQYVNKDWDAVRGLLGGKGANLAEMVRI
                     GLPVPPGFTVTTEACNAYQETKQFPEGMWEQMLDCLQLVEKSTKKKFGSTRNPLLVSC
                     RSGAKFSMPGMMDTVLNIGLNDETAKGMIKLTQNERFVYDSYRRLIQMFGSVVLGIPD
                     EAFEKHLDGLKQVKGYKLDTEMTASDLTGLIEVYKKVIFEEMGFEFPQDPLEQLKLAT
                     EAVFKSWDGKRAVDYRRATHIPDDLGTAVNIQTMVFGNMGEDSGTGVAFTRDPSTGEN
                     KLYGDYLLNAQGEDVVAGIRNTEKIAHLGRDLPEANKEFLQIAKKLELHYKDMQDVEF
                     TIEHGKLWMLQCRNGKRTALATVKIAIDLANEGIITREDAVMRVSPTDVDTLLHPQFS
                     SETKKLAVQEDRLFAHGVNASPGAAVGQVYFDADTAERRAKEYGESTIMVRPFTKPDD
                     VHGMLAAKGILTSEGGATSHAAVVARQFGVPCIVGAAAVKIDLDKREMNANGVLIKEG
                     DWISIDGSTGEAYIGKLNLTTPKFEEQIDLLNLLSWADQISSTKGIRHAPDGWPTTGL
                     QVWANADNPRDAERARTYGAVGIGLCRTEHMFFETERLPIVQRMILAKTSEERTAALD
                     ELLPYQRRDFEGLFEAMNGYPVIIRLIDPPLHEFLPSAEELQSKIIALRLSGGGLSNF
                     VLGRGELKETADLDTMLDAVNAMHESNPMMGMRGVRLSIAMPEIVEMQVRAIFEAAAN
                     IANKGMTPKPEVMIPLTGHVNELTWIQPRLERIAKAVMEEKKVKFDYKFGTMIEIPRA
                     AVTAGEIARVAEFFSFGTNDLTQMTFGYSRDDAERNFLTTYIEQEILPKNPFQTLDQE
                     GVGKLMQLAVQDGRLARVNLEVGICGEHGGDPASIDFCHRIGNNYVSCSPFRVPIARL
                     AAAQSAIKHRSK"
     gene            complement(31861..34263)
                     /locus_tag="PLW25_01305"
     CDS             complement(31861..34263)
                     /locus_tag="PLW25_01305"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOS53155.1"
                     /translation="MIKRALTSISILVFVLTSCTLPFNRTGENRGIPSGTNSAPQLSP
                     TPTITPTPQPQARITLGQADILNGDYEKALAEFWSAREQSTDPEVIQAAQLGVGRVLL
                     LQNDINGAVSQLNWLLTNFAEGESRDTAYFFLGQAYERLQQYLLAADAYQNYVDASPG
                     PLDSEILEMKGDALVRAGEFGAAKLAFLAALKTAQPIREDTLQLKIAQAAAESGGEQD
                     AISIYWALLENSSADYIKAQSNLLLGRLYLKLGMPEQAYARFQDSVTRFPTYYDTYSG
                     LVTLVEDGQPVNDLMRGIVDYYAGQYSLAVIALERYMLENPDHDGTPLYYKAYGYYRL
                     GDIDQEIAAWNELIEEHPFDQYLATAYLEKASSMWRYQQDYEGATNTLLHYAARYPDS
                     ARTPEFLFLAGKILEIGGYLTRAAETWQRVFNEYPGAEQAYPALFKAGIVYYRLQRYE
                     DARLVFQRMLVLSTSPEDQAAARFWIAKCLEAEDKDEQALTYYQQAADADPTGYYGIR
                     GGQKSRGEPPFPRQNNNDIAMDTDKEKKDAEKWLRATFSLDQGVDLHSYSDLSNNPLF
                     QRGEEYWKLGLRDNARNEFEQLRKELEGDAVNLYRLMNHMLDLGFYQTASLSSRQILD
                     LAGLSQDETLTKAPIFFNHIRFGVFYRDLVVPAAIEHGLDPLLIFSLIRQESLFDASI
                     TSSAGAHGLMQITGDTGETIVNNYGWPENYTVESLDRPFINVRLGTHFLKIWIDKYQG
                     EITPALSSYNAGDGNTIIWKGLAGDDVDLLVELIRFDETRDYIKYITENYEIYKTLYS
                     HP"
BASE COUNT         8107 a         8408 c         9368 g         8302 t
ORIGIN      
        1 tggagataca gcgtggcgac gtgttgttgt tatttaccgc gcgcttgtat agtgacatgg
       61 gcgaaatcat cgatttctca tacagctatt tcctgccagg gtacttcagg tttcacgtag
      121 ttcgaaatgt ggatacccac gtggcgtaca acgtttaaaa ggtgaaaaag acgcctgcca
      181 ggatattggt gaacgacgaa aacaaaccga tcgaaaatcc atttggaata acgagcaaag
      241 gaaaggaatg attcagcgaa aaggagggag cacacaaaaa ggattctgac gagtggaatt
      301 cttttctgta tgaatgagcg cggatgggat ttcgtctttg gcgaaaggct gctgtgcgac
      361 gcctgaatgg acaggagagc gcttctttgt tttattccct ggtggttcat tgaccgatga
      421 cactaaagat aaacttcggc ggtataatgt gaacttcagg gaagcaaaaa aaaatatgaa
      481 acaggtgatt gcatccgcgg acgtgaccct ggttgaacct atggtgcgga cagatcaccc
      541 caagcatggt gaagaagaag gagagcgtgg aaagacatcg gcggtcgttg cactcacaga
      601 cggatggttt attaataaaa gcccgttacg atgccactct gcttcattaa aaatagcatg
      661 gaagtggaac ctgccctgaa gtgtgagccg ttgggcaatc gcgttagcgg taaataagcg
      721 ttcaatgatg tggtaatgcg catagcgttt ctggcgcttg ttcttggaaa gatggaataa
      781 tcaactgatc aggaggaagg atgcatactt ttctacatgg acgtgatttg ataggggatc
      841 tggatttctc aaaggaggaa gttgagaccg ttttagatgt ggcgtttgac ctaaagcgga
      901 aacgcgcgtt gaacgaattg accccttatt tgagggataa agtgctcgct atgctgttct
      961 tcttcagcag tacgcgcacg cgcggctcgt tcgaagccgg catggcccat cttggcggac
     1021 acgcggcatt cattgagtcc cgcacgacgc agatttctca cggtgatacg ccaaaggaga
     1081 ttggcgagat ttacgggcgc tactttgacg gcatctctat ccgccactgt gattgggggg
     1141 taggcaacgg atacctgaac gaagtcgcca aggcttcgcg cgtgcctgta ctgaatatgc
     1201 agtgcgatat ctatcacccc ttccaatgcc tggctgattt gatgaccatt atggagaaga
     1261 agggccgcga tttacggcgc aaaaagatgg ttgtctcctg ggcgtacgcg gcctcctatc
     1321 aaaaacccat gtcggttcca caatctttga tcttacagat gccgcgcttt ggcatggacg
     1381 tggtgctggc tcatcctcct gagttcaggt taatgccgga gatcatggag caggcgcgcg
     1441 aacaagcccg caagttcgga gtgggttttg atgtggtcga agacatggac gaggctttca
     1501 aaggcggaga tatcatctat gccaaatcct ggggcgccat ggtgcatacg cctgaccctg
     1561 aagaaggagc caagattatt aagaagtatg agagctggat cacagatgag cgcaggatga
     1621 agttggcaaa acctgatgcg atttatatgc acccgttgcc ggctgaccgt aatatcgagg
     1681 tcactgacgc ggtattggat ggtccgcaat cagtggtata cgacgaagcg gagaaccgca
     1741 tgcacgttca agaagctgtg atggccttaa ccatgggtta agaacatgcg cagaaatcaa
     1801 atttaatttt aatagaaaag gaatttgtaa aaaatgacga aaaagttggc agttatcgca
     1861 gttggtggta attctctcat tctcgatccc aaaaaagtta ccatcgagga tcaatataca
     1921 gccgccgcag aaaccacgaa gcacattgcg gacatgatcg aagccggctg ggatgtggcc
     1981 attggtcatg gaaatggccc gcaggttggg ttccttctgc gccgctccga aatcgcggct
     2041 aaatatgaag gcatgcacga ggttccgctg gattcctgtg gcgcgaacag ccagggcgcc
     2101 attggctact ggttgcagca aaccctgcag aacgaattag ccagaagagg tatcaaaaaa
     2161 ccggttgcca cggtggtcac ccaggtgaaa gtcgacaaga atgacaaagc cttccagaac
     2221 cccaccaaac caatcggcgg ctttatggat gaagcagaag caaaaaagcg cgagcaagaa
     2281 cttggctgga acgtggtaga agactctggg cgcggttggc gccgcgtggt cgcctctcct
     2341 ctcccagtag aagtggtgga gattgactct gtgcgcgcgc tccttaatgc tggtgttgtg
     2401 gtcatcacgg ttggcggcgg tggtattcct gtggttgaca acggcaaagg cggttgtgtg
     2461 ggtaccgcgg cggtcatcga taaagattac gccagcagcc tgctctcaac tctcatcaat
     2521 gcggaccttt tcatcatctc caccgcggtc gaaaagatcg ccattaattt tggcaaaccc
     2581 gaccagaaat ggttggaccg catgaccctg gcggaggcga aagcctacct ggcagaaggc
     2641 gtgcattttg ccaaaggctc tatggcgccc aagatacagg ctatcatcaa cttccttgaa
     2701 gcaggtggta aggaagccct ggtgacaaat ccagaaaaca tcgggcgcgc gctgcgtgga
     2761 gaaactggaa ctcgtatcgt tcgtgaatag tcgcgaatga gaaacacgca agaagggctt
     2821 tgacggggaa aaccgcaggc ccttcttgat tttttaaggt ttttttactt gttttgctat
     2881 ggcattcatg agcgcatcgt gtacaataat tatgttgttt cctatcactc gcttggtggg
     2941 atggagggtc atcctatcaa gaatttacgt tttctagtcg aaaggaggtg caaaaaccaa
     3001 taactggttc cttgctttca cggtaagttg atgtttgaat ttgctaacta gaaacaggag
     3061 gagtgaacaa tgtttacaaa aaagttgtgg ttaagcctga gcgtgttatt ggtggcggtg
     3121 ctggttcttt cagcctgcgc tgcgccagcc ccgacggaag cgccagcacc tgaggttcca
     3181 gcagccactg aggcacccgc tgccaccgaa gcacccgcag cgacagaagc ccctgcgaag
     3241 gccccggtga ttgggctgtt actgatcggc cctgccaatg atgccggctg gagccaggcg
     3301 cattatgaaa gcatggctta cgtcaaagac aaaattcccg gcaccgaact gctttacctg
     3361 gagaatgtgt ataccgggtc cacctcgcat acacagactc ccgccgaact ggccgcggat
     3421 atggtgacca aaggcgccaa tatcatcatc tttaactcag atgatcaaaa agataacgca
     3481 attaagttcg ctcaggaaca cccggatgtg tttgtcattc atccttcggg agatgccgcc
     3541 tggaaagaag gcaagaatta cgctaacttg ccgaacatga agaacatcat gggcgagatg
     3601 gaatacggaa aaatgatcgc cggctgcgct gcggcgctca caacgcagac aggaaaaatt
     3661 ggcttccttg gtccattgat caatgaggaa acacgccgct tcgcggcttc agcgtacctc
     3721 ggcgccaagt actgctggga aaactacctg caaaaagatc ctgctgattt ggaattcaag
     3781 gtcacctgga tcggtttctg gttcaatatc cccggcgtga ctctggatcc gacccaggtc
     3841 tcggatgact tttacaatac cggatatgat gtggtgatca gctcgatcga taccactgaa
     3901 gcgctggtgg aagccaagag gctcgctgct gacggtaaga aggtttgggc ggttccttat
     3961 gactattacg gcgcttgcga aggcgcgcaa gaagtctgcc tgggcgtgga atacttcaac
     4021 tggggcttcg cttatgtgga tgccatcaaa tctgccatgg acggaacctg gaaatccagc
     4081 tttgagctgg tgccaccgga ctggaaggat atcaacaacc ccgatacttc gggtgttggc
     4141 ttcatcaaag gtggcggtct ttccgcggaa aatgctgcca aggttgacca gttcatcgct
     4201 gaactgggag ctggcttgaa cctgtggaaa ggtccattga acctacaaga tggcacggtt
     4261 tacctggcgg atggtgaagt ggcaacaatt cagcaaattt ggtacctgcc gaaattgctt
     4321 gaaggaatgg aaggtcagag cgagtagtat agaacgccgc gtgggggaag ggtttccctt
     4381 cccccacgtt tttataaata cgaggttcac gcttgaaaat ttcattttca catattcata
     4441 aacgttttgg aaatgtgcac gccaataacg atatcaacct ggtaattcca tcaggaagca
     4501 ttcaaggcat cctgggtgaa aatggcgcag gaaaaagcac cctgatgaaa atccttaccg
     4561 ggtactacca cgcggattca ggcgaaattc tgctggatga ccgggtggtg aagatcacct
     4621 cacctgtgga cgcggtaaga cagggcattg gcatgctgca ccaggaccct ttggattttc
     4681 cacctatgag ggtgatcgac aacttcctgc ttggctccag aggcggttta tttccaaaac
     4741 gaagtgatgt ggaaaaagag atccttcggt tatcacgaga attcgatttt cagatcagcc
     4801 cggatactta tgttgaaacg atgacggtgg gagagcggca gcagcttgag attctgcgac
     4861 tgctgtggct gggcgtgaag gtgctgatcc ttgatgaacc cactactggc atttcagcca
     4921 atcagagaga aaagttattt gccaccctgg ttaaactggc taaacaaggt ttgactgtga
     4981 tttttgtttc gcacaagctg gaggaagtcg agcagttgtg cgaccgggtg gcggttttcc
     5041 ggcaggggga agtcgttgga gaagtcgaac caccctatga cacggatcaa tttgtgtata
     5101 tgatgtttgg gaagtcagtg gaagtggggg ataaaaaaac attttgcact gagctgccgc
     5161 tcattcaatt agaaaatatc gggttggaag attaccgcct gcacctcggt aacatcaacc
     5221 ttgaaattta ccagggagag gtcatcggcc tggcgggtat ggaaggctct ggtcagaggc
     5281 tgttcttgca tatgctgacc ggcttgctgc gccctgtgaa cggtaaaatc aaattgaccg
     5341 gtgaagattt tactggtaaa acctatccct atttccaaaa ggccggggtg gcctatctgc
     5401 cggctgccag gctggaggaa ggtctgatcc cgggaatgac gttgaccgaa catttcatcc
     5461 tttcagaaga gcatagtgaa ttatttgtag atcagaaaag cgctttgggc ttgacagaag
     5521 aaaggatcag ggacttctac atcaaaggaa ccccggtgag tatgatcgag cagctttcag
     5581 gcggcaacca gcagcgtgca ttgctggcgt tgctgcgcag aaaattgaaa ctgatcttga
     5641 tggaacatcc gacccgggga ttggatattg aatcggcaat ctggatttgg tcaaaaatga
     5701 aagagcgctg taaagagtgc tccagtgtgg ttttcacgtc ttctgacctg gatgaaatca
     5761 ttcagtacag cgatcgcatt ctggtgtttt ttggcgggct ggtatcagaa ccaatcgacg
     5821 cgactaccac aactgtggat gagttgggtc aactcatagg cggtaaggga tggtaattat
     5881 gtgtgacgga gaatctggat gtctgtagaa ctgagataca gccgatcagc caggtcattg
     5941 aaaaaaagtg cctgggtaaa tgtggggatt cgcctttctg ccattattat ggccttattg
     6001 ttcacgtcgg tgatattgct cattgccaag gctaatccat tgcaggctta taaaagtatt
     6061 cttcatggcg cttttggttc taccattaaa ttgacctcgg tgattgcctc gtgggtgccg
     6121 ctggtgatcg ccacttcggg attgctgttt acctttaccg ctggattgtg gaatatcggc
     6181 atggaaggtc agatcattat ggggtcggtc tttgcggttg gcgcatttcg ttttttagat
     6241 gacacctcgc tggcgcccgc tctcgttatc accctggcat tcgtcgccgg cgcggttggc
     6301 ggaattctct gggctttgtt acccggttta atgaaaatat ttggcggtgt taacgagata
     6361 tttggcgggc ttgggttaaa cttcgtggcg aaaacagtgg ctatctggct catctttggc
     6421 ccgtggaagc gccccggtgt ggcatcgggg agcgggacgg aatggattga tattcaatac
     6481 cgcctgcctt acctgccagg aaccaatctt agcctgacga ttctcattat cgctgtcatt
     6541 atcgccatta ttactgcggt catgcttaag ggcacccatt ttgggctgcg cctgaaagcc
     6601 attggcaaaa atgacaaggc cgcttacatc ctcggtgttc ctacttcccg ttactttctg
     6661 ttggctttcg ggatttgcgg cttcttcgcg ggcatcgcag gatccatcct ggtgacttcc
     6721 aagcaataca gccttatccc ggacgccgga tttggatatg gtttcctggg cctgctcgtg
     6781 gcaatgcttg tcaactacca cccgatttgg tcgtcagtgg ttgcgctctt tttcgccgcg
     6841 ctcagcatcg gcaatacaga actgcaatac atgggtctga actcgtattt cgccggtgtg
     6901 ctgcagggtt tcctggtttt atttgtggtg ctggtagatg gcgttcgcaa acaactggta
     6961 aaaacgcgtt aggagcggct tgatggaaat gacaactctc acaacactgg cgggaatcgt
     7021 tgaattcgcg gttccggtgc ttttcgctgc gattggcgag aacatcaccg aaaaagccgg
     7081 tttgataaac ctttcagtga atggcacgat aatcctgagc gcaatgggcg gattcgtagt
     7141 ggcgctcacg accagtagca tcttgctggg tttccttgtg ggagcggtca tcggagccgc
     7201 ggtggctgcc atagtggctt acagcagcat cacgctcaaa caatctcagg tagcgattgg
     7261 atttgtgctg gcaatgcttt gccgtgacct ggcgtatttt ctcggcgcgc ccttcaccaa
     7321 caaacctggt atttttcttt ccaaagtacc cattccctat cttgttgata ttcccatcat
     7381 tggcccgatt ttcttcaatc attcactggt tgtttacgct tcatacctgt ttatcatcat
     7441 cgcgtatgtt tatgtgttca aaacgcgtcc gggtttaatg ctgcagggaa ttggcgaaaa
     7501 accagcggcg gctttcgccc gcggggcgaa tgttaaaggg ctgcgctatt tgtacaccat
     7561 cactggcggt gcgttgatag gcttggctgg gccgatgtat tccctggctg tgaagatagg
     7621 ttgggcgggt cagctttcag ggctggatgg gatcgggtgg atcgccctgg cgataaccat
     7681 ttttggcggg tggaacccgt tgagggtggc aattggtgct tacctgtttg gcggcttgca
     7741 acaattgggt atcaccctgc agtccgcgac aaatattcca attcagattt tacaggccgc
     7801 tcctttcccg ttgatgattt tcacactctt gttggtcaat gtaggccggg cggattgggt
     7861 agaccgggag cttgccgcca tgcctgaaag accgcgcaag gttctttcaa aaatactcca
     7921 cgcgctgcgc acctcgcccc cttcagccat cggagtgcct tttgagaacg aataagcagg
     7981 cggcatgaat cactacctgg gctacaggtg cacgttttgt gatactgaaa tttcccccac
     8041 ggaaattctc tatacctgcc cggcgtgcgg cgggaacctg gacgtggtac tggattacag
     8101 gcgcatccag cagaagtatc gcccggagga tatcttttcc agggctgaag gctctttatg
     8161 gcgatacgag ccgttactgc cggtaaccga tcccggtggc ttagccaccc ctcttcacgc
     8221 ggcagggtgg acccctgttt accagccaga gaggttaata cgcggcctgg gtttgcggaa
     8281 attttggctg aaagacgaag ggaaaaaccc taccgcctct ttcaaagacc gcgccagtgc
     8341 tattgtggta gccaggtcga gagaaatagg cgctgatgtg gtggtaaccg cttccactgg
     8401 caacgccggc gcggcgctgg cgggtatggc agcggcaatc gaacagaacg ccgtaattct
     8461 cgcgcccaaa accgcaccag cagcaaagat tgcgcagctc cttatctacg gggcacaggt
     8521 tgttttggtg gatggcagtt atgatgacgc tttcgacctg accatccagg cttcgcgaga
     8581 attcggctgg tattgccgta ataccgggta taaccctttt acagccgaag gcaaaaaaac
     8641 cgctgccttt gaaatatggg aacaggtact gcgtcacttt cgcgcaggca ataaaccatt
     8701 atgcgttttt gtctctgttg gagatggcaa catcatttcc gggttgcata aagggttcaa
     8761 agacctgatg gctctagggt ggctggagcg aatgcccagg ctgtttggcg tgcagtcgga
     8821 gctgtcttca gcggtggcgg acgcttacct ggcgggtact gaggtgatca caccagtcaa
     8881 ggcgaccacg ctcgcagact ccatcagtgt gaacctgcct cgcgatggcg gcagagcagt
     8941 gcgtgcggct gtggaaacag aaggagcgta tattaaggtc gctgacgccg atatcatccg
     9001 tgcgatcatc gacctggggg cagcaggcat ctttgccgag ccagccgggg cggctgcctt
     9061 ggcggggctg caaaaagcgc ttgctgaagg agatctcctg gaggatgacc cggtgctggt
     9121 gatcaacagc ggaaacggtc ttaaagacat tcgcgcggcc atgatggcgg ttccaaaccc
     9181 ggctataatt gaaccgacca tgtcagcctt aaaacttcac cttgagaaaa aaggtcgtat
     9241 tgaatgaaac cagttttttc gattattgcc ccggtctata acgaaatcgc ctgcctggat
     9301 accctgctgg acaggatcca gaaggtgatg gaccaaacag gagagccatg ggagcttgtc
     9361 ctggtggatg acggctcgac cgacggctct acggataaaa tccgccagct cgctgaaaat
     9421 gaccagcatg tgcgtaaggt gattctcgcg cgcaactttg gtcaccagat cgctgtgaca
     9481 gccgggatgg actactcccg gggtgatgct gtggtgatta tcgacgcgga tttacaggat
     9541 ccgccggagg taatattgga tttaattgcc aggtggcgcg agggatacca ggtggtatat
     9601 gcccagcgga ctgagcgcga aggggagacc tggttcaaac gcgtaactgc atctgttttt
     9661 taccgctcta tttaccgcat cacggatatt aagataccac tcgatacggg agatttcaga
     9721 ctactcgacc gtaaagtggt caatgtgatg aacagcatgc gcgagcggca ccggttttta
     9781 cgcggcatgt cagcctgggt ggggtttaaa tcgatcgggg tgccgtacaa acgcgcggct
     9841 cgttttgccg gctccaccaa atacccgttc aagaaaatgc tcaagcttgc cctcacggcg
     9901 gtgaccagtt tttcttatgt cccattgcag ctggctatgt ggctggggtt catttcagct
     9961 gggatcagta tcatcaccat tcctgtggtc attgtactca ggctggttgg atcgcaattc
    10021 ttttttggac aggcgacaac gctcatcgct gtgcttttcc ttggcggcgt gcagttaatc
    10081 tcgcttggta ttttggggga gtatgtcggc cgaatttatg atgaagtgaa agggcgccca
    10141 ttgtacacaa ccagcgaaac cccagatgat gcactgaaaa catcctgaca ggagagacaa
    10201 cctcatggct aagatcgacc tacaaatctt tgaagaacgc cgacagcgcg ccattgaacg
    10261 ttgccggaag cagaatatcc tgtttccaac ctttgctcaa atgaagaacc ctgatctcat
    10321 cccgcaaaaa atcaaagagg aactctcttc aattggtttg tgggatattc atccccgcaa
    10381 cctatttcgc attacctgga aaaacgaacc tttggaaaaa ggcggcggat acggtggagc
    10441 gaattattta gagcttccaa ctgaattaac cggggttaag gcgcggatca tcgccctggt
    10501 gggcaaatgg tttccaaccg gcgcgcacaa ggttggcgca gcctacggct gcctggtgcc
    10561 ccggctggtt acggggcagt ttgacccgac gcttcaaaag gcggtgtggc cttccacggg
    10621 taattactgc cgcggcggcg cctatgactc cgccttaatg gcttgccagt caattgccat
    10681 tctgccagag gggatgagcc gtgagcgctt tgaatggctc aaccgtgtgg ccggagaggt
    10741 gatcgcgacg cctggaacag agtctaatgt taaggaaatt tttgataaat gctgggagct
    10801 gcgccggtct ggtgaagatt tgatgatctt taaccagttt gacgagtttg gtaattatct
    10861 gtggcattat gaggtcaccg ggcatgctat ggaggaggtg cttaaaaagg aaatgcgccc
    10921 aggcgacagg tacagggggg ttgcgctgac caccgggtct gccggcacca tcgccagcgg
    10981 cgattactta aagcagttgt ttccggaaag caagatcatc gccagcgaag cgctgcaatg
    11041 ccctactttg ctcgaaaatg gctttggggc ccatcgcatc gagggtatcg gtgataaaca
    11101 cgtgccctgg atccataaca taaagaatac ggacctggtt gtggcgatcg acgacaatgc
    11161 ggtcatggaa atcatgcgtg tttttaatga gcctgagggt caaaaatatc tggccagcct
    11221 gggccttgca gaagccctgg tatctaaatt acacttgttg ggtttttcag ggatcgccaa
    11281 cctgctttct gcgattaaat ttgcgaaata ctatgagctg ggagaagagg atatcgtcct
    11341 caccgtcctc acggactcta tggaactgta tgaaagccgt gttgttgaaa tacaaaaaga
    11401 aaaaggtaag ctcactcaac tccaggctgc tgctgctttt tctcgctacc tgcttggcga
    11461 gaggactgac aacctgatcg aattatccta ccaggacaga agacgggtgc acaatctgaa
    11521 atattacacc tgggttgagc agcaggggaa aacatacgaa gaaattctgg atcaatggta
    11581 tgaccctacc tactggacgg acgttcaagg tcaggcggaa gagattgatg aattgataat
    11641 tgatttcaat cagcgcactg gactgcttta gtatttcgct tatcctgaga atctttgctt
    11701 tcagggcgag attaattaaa ctgaattcaa atgcaaagaa gcatctctcc taacgcgcta
    11761 ctactgctcg gaattctacc ctcgcccagg gatcttgaaa ttgcccgggt gctgggatgg
    11821 tatcgtgttc cgctaaagtc ctctcccaag gtcattgaag tggactacct ggcgttttat
    11881 cagggtgcga actttggtga gcaacatcgt tggaaggttg aatttttcgc tgagtaccac
    11941 ggacatgaac tgaccacacg agcggaattg ttgcgcgacc agccagacca tcccagggcg
    12001 aacgaagaat acttcaagat ccaactgggg ccaattctgc cagttgaacc tccaatcgtc
    12061 tccgctggct ggaaacgtat cacatttctt tttaccacgg gtgaattatt caaccaggct
    12121 gaaacgatca atgacctggt cgtgcgcaca gaagagcgcg ccgtcttgtg gaaaaaatta
    12181 cgcgagcgaa accgggagat gaatcgctat cgcgcagggg cttctccgaa tgcggagatt
    12241 gcgcctgagg tattggacct gctgttgggc ttgaattcta atcttcctga caccgacctg
    12301 gaaaattatt aaccgcccgg ctaatgctgg tagaataaaa aaagagaaat acctaggcga
    12361 gtcatcagga aaacaagatg caaacattga taaaaaacgg tgtattggca accgctacga
    12421 aaaccgaaga agcagatctg ctgatcgagg atgacaagat catcagcgtc gggcagggct
    12481 tgacggtgga agatgcggtt gaagtcatcg atgcccgagg attatacgtg cttccgggag
    12541 gcgtggatgt tcacgtgcat cttgacctgc cgatgttcaa taccgtgtca tcagatgatc
    12601 attataccgg aacaaaggcc gctgcatgcg gaggaaccac cacggtgatc gatttcgttt
    12661 cacaagaaac agatgacctg ctgggatgcg tggccaagtt gcggcagaag gcagatgcca
    12721 gggtggcggt tgattatggg ctgcatatga atattaccca tctcaacccg cgggtcgagg
    12781 agcaaattcc tctcttggtc aaagaaggcg tgaccagcct gaaagttttt accgcctaca
    12841 atggacggct gcgcctgcag gaccatgaaa tcttccgtgt gctgaggata gccggtgaac
    12901 atgggctttt gacgatgctc catgcggaaa acggtgatct tatcgagctg ctggttcagg
    12961 aagcctgcct gaacgaccga aaaagccctg tttggcatgc gcggaccaga ccggcttggg
    13021 gcgcagtaga ggcggtgatg cgcggggctg cgttagccgc tgttgcccag gcgcccttgt
    13081 atatcgtgca tatcaatacc tccggagaag tggatatgct tcggtatgcc cgcgatcatg
    13141 gtgtgcagct gatgggtgaa acctgcccgc aatacctgtt ctttaccgaa gatgaccttg
    13201 cccgcccaga tggcgcaggg tgggtttgct ctccgccatt gaggactgcc gcagaccagc
    13261 agcgcatttg gaaaggtctg aaagaaaaaa cggttcaggt catttcaacc gaccattgcc
    13321 ccttcttttt gaatggcacg aaaccgattc tttacgaagg aaaagaggta gccatccccg
    13381 gaaaggagtt gggacgagac gatttcacca tgattcccaa tggcttacca ggtgttggcg
    13441 accgtttacc ggttttgtgg acgaaaatgg tcggttctgg tgaattttcg atcaacgagt
    13501 ttgtcgcact taccagtacg aaccctgcca ggatcttcgg tatgtatccc cgcaagggtt
    13561 cgttatcacc cggcgcggac gctgacatcg tgttatggga tccgcgtttg gaagtggtgt
    13621 atggggtggc tcacgcgcaa cagcgcacag actacaacct gtatgaaggt tggaaattaa
    13681 ccggttaccc gctgcgggtg ctttctcgcg gccggactgt gtactgcgat ggaacctggt
    13741 caggtaagcc gggtaatggt aagtttattc accgcgctgc attcacaggg atggtttagg
    13801 aagcgcaatg ttgtctttct taccggttat tctcctgttg ctttcagctg ctgcgatcca
    13861 ggttgtcaga aggatgacca aacgtgcagg cagcacctgg ttaatggcca tgatatttgg
    13921 ctctgtcacc tggttgagca tgatccttgt gggtgtgctc ctccctccgg cggtcaagat
    13981 tgacaactgg ctctctttac ctcaccagtt caccgcgata acatttcagt tgaccagaga
    14041 aacctgggtc ttgggttttc tgcttgtttc tttattgcag gcaattatct tgttcaattc
    14101 aaggtatata gatacgccta atttcctcaa caagatcact agttctatgc ttctgacggc
    14161 tttcgggtta atggcggtat tggcgcgcac gccgctcgca tttgtgttga cctggtcttt
    14221 gatggacctg gctgaattcg gcgtcttaac gattaccctt gaccagacct tgcaaactcg
    14281 ttcatcgttt gcggctgttt tgttacgcga actggggatc atcttgctgg tgctgttgat
    14341 ggcggtttca cctgagcaga acctgcaccc cggagatacg accccgactc ttatcgcctg
    14401 gctgctggga ttgatttttc tgttaagact gggtatcctc cctatcctgc cagaccagga
    14461 agatgattct cggctgcgaa ggggattccg aaccttgtta cgaactcttc cttttgtaac
    14521 ggctattgcg tttgccagta ttttccctgg cctgacgctc gctgaaaaag gttcacaaat
    14581 tgccctgcct gccctctcat ttgccgggtt attggctgct attttgtggt ttttttcacc
    14641 ggacgagctc agcggtcgcc cttattggtt cttcgccaat gggttacttg ggctgatggc
    14701 gtttctcgtc aacagggtgg aggcgctgcc ggggatcgcg gtaattgccg tttcagccgg
    14761 cagtggcctc tttttgtatt cacctaaaac gttgaagaga ttggcctttc ttcctttctt
    14821 actggttggc ttgctcgcct tacctttcac tccgacggtt ggcatcctct ctttctttac
    14881 tcttggaatt tttcgcttca accaggtgct ggcggtaacc acgtacgcgt tgctgctggc
    14941 cggagtactt aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcagca
    15061 tattgaagcc aataccatta aatcaggcgc tggcaggatc ttttacagtg tgagttttag
    15121 cttttgtatc cttttggtct ttttcaccat tcaaaaaaca acccgctttc tggcgattgg
    15181 tcaggatcag agcatgcccg ccctgttggt cctgttatgc ggtttcatct tgttgggaat
    15241 cgcgcagcat ccgtttagaa tcattatggg gctgctcacc atcattaccg gttttgaggt
    15301 gttatacagc gcggttgaac agtccctctt ggttaatggt ctcctggggg ctgtagtgct
    15361 cctgattgca tgggtaggtt cgtatctgtt gatgccaacc acccctggag aagagcaatg
    15421 agcgccccgg tcatctggat tgtttttccc cttgttgttg cactggggca attgttcttg
    15481 ttgaaatatc caacgatatt gaaattaaca ggcatcgtgg ttagccttct attggccctc
    15541 atcgcgtttt ttcaaccgat cggcaatgta ttgcgtcttg gcggcatggt cctggatatc
    15601 cgcccggatc ttcaattttt aggcagggca ctccatcttg ataacggcga ccgttttatc
    15661 ctggccgggt tgtttgggat tttgttcatc tatacttttt tgtggaatac ccatactgtt
    15721 ccagccaagt ttgcgccact ttctctcgcg attagcgcca tcctggtggc ggctctggcg
    15781 gttcaaccct tcctttacgc cgcggtgctg gttgaactgg ctgtgctgct gatgatcgtt
    15841 ctggtcaaag acaatcagat gaatcaggag aaaggtgtta tccgttttct ggtttatctg
    15901 acactcgcga tgccatttat ccttttttcg gggtggatac tgggaggaac ccaggccagc
    15961 ccttctgaac aggggaattt gctgggagcg gccatcacgt tgatgattgg attttctttg
    16021 tggctggcag tttttccttt tcactcctgg gtcccacaat tttcgcgcgc cgttcagccg
    16081 tatctgtctg gtttcattat cagtcttttc ccgatcgtgg ttctctttgt cgcagtcaac
    16141 tatatctcca gcctggtttg gttgcggtca gcctcttatc ttgctccagt tctcagcagc
    16201 acgggtatca tcatgattgt taccaccggg ttgtgggcca gcgtagaaag agatgtgaaa
    16261 cgtgtgttgg gttactgtgt actgctggaa acaggttttt cattaatcat gatcagtttt
    16321 aaatcagagc agggtttgat cctgatgtat cattcacttt ttccccgcgt attaagcctg
    16381 gcactgatgg tgtactcttt atccattctt caccaggcgg gaattgccct tactcacgac
    16441 ggtttccaag gggttataag gcaataccca tttgctgcca gcggtttttt gatctctttg
    16501 tttactattt caggttttcc tctttttgcc gctttcccaa ttcggctgga actcctttac
    16561 cagttgggtc acatttcggt gccgtcggtg atttggacag tatttggcct ttcagggtta
    16621 atctttgctg taatccggtt gttcagagtc atgactttac cagcgccaaa taattggcgt
    16681 atccatgaaa gcgttactca ggttatcatt atctgtacgg ttattcttgt gctcatcctt
    16741 gcaggtttat ttccgcaatt ggtggagggc atcattaaac catttacggc agatctaccc
    16801 ttattaaaat aaatgcccac gcagtgaaga aaggttgaaa aatggaaaat gaagaaatcg
    16861 taaaacgaat tgaatggctg gatggagagc gccgtaaaga caggcaaacg attattgagc
    16921 tgcaggcgca ggtttccaag ctgcaagaag tgatcgcatt gcaaaaacga gagttgtcca
    16981 acatcaataa ggataacaag aagtcttttt ccagtgtggt gcgtttagat gaatacgaag
    17041 agacgaacgc gaagctaaaa gcggagttgg ctaaaagcca gcgattgatt gaaaaacggc
    17101 tcgacacaat ggagcaaacc actgcagcgc aaagaagcga tgaccgcgat acctacaata
    17161 aaagaatgct cgaactgcag ggcgaaatta aggctcttaa cgaattgaaa aaaagcgtgc
    17221 agtccaaggg agaagatgac ttccggctta atcaaaagct ggaagatctt gccaggcagc
    17281 ttgctgactt gcgttcttcg ggtgatgaac agaacaggtt ggcgaagatc ctggatgata
    17341 actatcgtgt agagtcaaaa agactatcgg atttacagat tgagttgaac acgttgcgca
    17401 aacgcatcga tgaggatcga aatctatctg atgcccaaaa agagtatctc aaaaagctgg
    17461 aaggtcagat ccacgacctg gtgaatcgag aaaaaaccag gaaccaggag caaattgagt
    17521 ttatcgaaaa acagtctctc ttatcagtag atagagaaaa cctttggaag gactggcagg
    17581 aagaaataca gcaaatgaaa gcattgtcag aaaacttcca tggtcaggta cgcgaactgg
    17641 aaacgcttaa tcgagaaatt aaacaggggc aaagcgattt tgaggtaatc aaccagagac
    17701 tggaacggag aatcaacgaa atcactgaaa tgaaccgcct gacggaagaa agattcaggc
    17761 aggaatggat tgcatttaaa gcggacgacc agaaacgctg gaccaattat tccttaagcc
    17821 aggaagaaac caaccgtgaa gggaaccgcg agatcaacaa gatagatgaa cggataactt
    17881 cgcttgaaga cgcggtacaa aacctgttgg ataccgtcca agtgattcac gaggaaactg
    17941 aaaagagaat aaaaggtctg ttgatgcttg ccaatgaata catcacttct tacgagcgca
    18001 cagctttgaa aaaaggatga gtgatgcggg tcattggaac tgccgggcac gttgaccatg
    18061 gaaaatcaac cctcgtcaag gccttaaccg ggattgaccc agaccgtctg aaggaagaaa
    18121 aaaaacgcca gatgacgatt gaactgggat tcgcctggtt tgaatcccaa tcagagggcg
    18181 aaattggcat cgtggacgtc ccgggtcacc gagattttat tgatacgatg ctggcgggcg
    18241 tgggtggcat tgacgcggta ttgctggtaa ttgctgcgga cgaaggtatc atgccgcaaa
    18301 ccaaagaaca cctggccatc attgaactcc tgcgcgttaa taccggtttg gtcgtgctga
    18361 ccaaggttga cctggtggat gatccggcgt ggttggaact ggtggaaatg gatatccgcg
    18421 agagactatc gtcaacctgc ctggagaacg cgccgctgtt gcgcgtatca gccgtgacag
    18481 gagacgggat tgcacagttg agggcggcga tcgaagaagt attggcacct cttccgccgc
    18541 gccttgatct gggtcgaccg aggttgccca tagaccgtat tttttcgtta accgggtttg
    18601 gcacggttgt gactggtact ctgctcaatg gctctttgtg cgttgacaat gaggttgaaa
    18661 ttttgccttc ccggcgttcg gcaaggatcc gcgggctgca aactcacaag cgcagcgagg
    18721 aaaccgcgct gcctggcagc cgtactgcgg tcaacctcag cggcgttgat gtggctgaca
    18781 tcaagcgagg agaggtcatc accttacctc gattgttgga acccactcaa cgcattgatg
    18841 cgcaaattga attattacaa gacgcgccag tttcactcaa gcataatgac ttggtgaagg
    18901 tgtttgcgtt gacatcagaa tgcgctggca gggttagggt tttgcagggc caggaattga
    18961 agccaggtga gaacggttgg gtgcaaattg agtttgaaaa agaaatggtg ctggagaaag
    19021 aagatcgatt catcatccgg cgcatgtctc ccgcgcacac gctcggcggg ggagtggtcg
    19081 tcaaccctca tccaggcaga cggtacaggt tgcaggattc aagtgtgatc gaacggctat
    19141 ctctgaactc cggtactgcg cttgaaaacc gcatactggc gctcctcatg gaaaaaccgt
    19201 tcattactgt tgatgagatt caaaaccggc tcgagaccga ttcagagcgc gtgcgtgaga
    19261 tactggctga gttgaccggg cgagggcagg tgagcgtgtt agcagggctt acggatcaaa
    19321 caaccgggta tttgccgcgg gcgaagtggg cagagttgac cgcgaaatct gtacaattgc
    19381 ttcagcacta ccatcaacag aatccgctga aaagcggcat taacgtggat gagcttgccc
    19441 ggcggttgag aatctcacag gacgagttga atttatgcct gcgagaatgg cttaaagaga
    19501 aggtggtgaa gctgcaggat ggagccatcg ccctgcccgg ctttgaagtc aggtattcca
    19561 gcaatcaatc gcggaggttg gagcaattca cagctatact cgatcagcag ccttttttac
    19621 ccccaggagt gaaagagagc aaggacctgt taacagatga tgtctttcaa tcactcgtgg
    19681 agcgaggggc gcttgttcag ttatcaaccg aagtggtggt gcggaaggct gaatatgatc
    19741 tgatgctggg ttacgtcatc gagcgttgtt cccgtgggga gttattgacg ctggctcagt
    19801 tccgcgatca cttcaataca aatcgaaaag tatctcaggc tttcctcgag ttcctggacc
    19861 gtaaaggggt gacgcaacgg gttggagaag ggcgcgtgtt gaaatagaaa tcccccaaaa
    19921 acacttcact cggtgctgaa taataggtgc tgaccccctc aggtgctggt tcatctgcaa
    19981 ctgtcaaacc aaagcatagt gtactgaacg attattttcc tttataaaag cagtaaagac
    20041 attcaccttg agagaacgag tgggtaaaat ttgctcaatg aatttttatc tattctgtgt
    20101 aaacgcccaa gtcgtttcga ttgcaagaaa aaatcagtgc acaatgagac acatcgaata
    20161 tacagcatct ctgcccaaca tcgtctgcag cgcaccggct tcggcgtttg cccgcgacgg
    20221 ctatttggta gaaaagtagt tttcagagtt agctggctcg ctttgacggg tggcaggtga
    20281 gccatactgt tggcagagta tgtcttgcgg cagggcgctc gattttgcgg tcagggaacc
    20341 tcgtcctgat cctgttcgca taatgtctct tcgatgtgac tcaacaggtc agataaattc
    20401 tcgcaaaaga acacttctcc gctgcgaata ttctgcaaac ggctgcgcca cttcggcgca
    20461 gccgtccctt cgttcaccgc ccagatccgc agcgtgaaga gatgcgttcg cgccccggct
    20521 gggttccctt ccacgcgcat gagcgttatc ccttgcggtt gaagaccacg aacagaaccg
    20581 cgaagaccgc agcgatgatc atcggcggac cgacttgcgc gaaggtcgag atgccgttca
    20641 tcaactgcca aaggaagacg acgacatgcc ccacctccgc aaagaccagc gcccagacca
    20701 ttttgcggag cgcggcggcg tccgaccgga tcagccaggc ggccaaggca aggaccagca
    20761 ctacctggcc gtagagcatg gtcagcgcgg cgtcgggcag gcgcgtctga aaggcggttt
    20821 cggcgatgcg cggagcgaag agaaacagaa tgccgatgat ggcggtgtag atggtgaaga
    20881 cgagcagggt aatgcgtaac gctttcatga aaacctccgt aaaactatgg tttgacgact
    20941 atccatagtg tacggatgga gcgtttcaaa agcgtttcgt tttaaatagc agccttaggg
    21001 gtgaaaatgg gatttgatat ctcgttttcg atctccgatg ttttgttttt gaatatttgc
    21061 tgttcgggaa ttgggaatcg aagttgattg tgtcaattcg tggatgcgat gggaggtcgc
    21121 agccacctcg acctacaggt atccccgctt ctttgcccac gctaacgcct gtgccagcaa
    21181 gggcgcggcg ttccccacgt ctgcagatga ggcttgtatg agcagatcgt ctccttcttt
    21241 ctcaaagatt tgctgatgcg gctccttcaa cttgcgcgca tactccagcg cttcatcagt
    21301 acgcagagag tgcaagctca ggtcaatcag cgtccacaag gcgagccagc ggaagggata
    21361 ccccgcgccc cacatctcca gcgcggatag gctgagcgcc tttgcttgac gcagattact
    21421 ccgccgccac gccgcccagc ccaggttggc gcgcgccgcg gcgacatacg ccaggaagcc
    21481 ttcgcgctcg ctcaacgtca gtgcgcgttc agcgcaggtc tggcattctt ccacccgccc
    21541 cgcgccacga tacgccgccg ccagataggt gacgctcatc agctgcagtt ccacgtaacc
    21601 gatctgctcc gccagcctga gcccctcgcc gagataatga atcgcctcgt cgaaatcccc
    21661 gcgcaggaaa tggcaaaagc cgtagccgaa gttcgtgcgc gtttgcaatt ccaggttgtc
    21721 ggtctttgcg ctcgtctcca acgccagttt ggagtaacgc atgatttcgt cgttgatttg
    21781 atagccgtcg cggcggaagt aggcggtggg gaacaggaaa taatactcgc ccagcttttc
    21841 caattcaccg agacgctcca ccacagggcg gatgggcgcc agcgcggcct cgagcgcctc
    21901 cacccggttg gcgtcgtact caacccacac gcggtcaaaa tggaccttca gccaggcgcg
    21961 ctgccagtct tccgcctccg acggttcgcc gagcgccgcc tcggcttgcg cgaaaagatt
    22021 cagcccctct tgcaggttgc cgtgattcgc ctcggcacgg gcgagtttgc ccaagacgcg
    22081 cgccagcgac agcccgtccg atgactcaac gagcgtcagc gcttccctgt atcgttccag
    22141 cgatttttgg ttgttgccca tctcgtaaat atcgccgagc gcttcgcata tttcgccagc
    22201 cgatttcttt tgctcctcca tttcggcgcc gattttttcc ttgagcagtt ccagcgcgcg
    22261 ctccaaatgc gccagcgcat cggagttggc aaagacccgc cgcgctgcct ccgctgcgcg
    22321 ccgataagcc tccaccgctt gcgcagactg cccgcctgca tcccagtgac gcgcaataac
    22381 ggcgggatct tcccgctcca cctccaacga ctcggcaatc ttgcggtgaa gcaactgctg
    22441 ccgcggcgcg ctcaacgcct cgtatgccac ctcctgcaat tttccgtgtg taaagttgta
    22501 ggcatccgcc cgctggattt gaatgacgcg ccgcagccac aattcgtcta gcgcctgtac
    22561 cagcacggac tcgttcatct ggctggcgcg cgctaaaagc ggatagcgga actcgcgccc
    22621 gatgacgccc gccacgccca ccagttcgcg cgctgccgcc gagagtccgc ccagccgccg
    22681 ggtcaacact gaccgcagcg tttgcggcat ccccgtctcg ttgattttcc cgctgcgcag
    22741 cgtctccacg ataaagagcg gattgccctc ggtctgacgg aagagttcgt cctcctccgc
    22801 cttctccaaa tccctgccca acacatcccg cgccagcaga cccaattcgg gcttgtccag
    22861 cggcatcaat tccagttcgt gcagttgctt gcgttcttgt agagacagcc gcagctgggt
    22921 tagcggatgc tcctcgtcca gttcctcaac ccgtgccgtc atcaaaatca ttagcgccgc
    22981 gttggcgtcg tagcgcatca ggtaatggac gatttcgagc gtctcgctgt cgctccattg
    23041 cgcgtcgtcc agcatcagca ggcgcggcgc acccgatccc agcaagcccc gcgccaccgc
    23101 ctcgaacagc ctgcgccgtt cccactttcg cccgctcgaa ggcgttccgc ccgccgcgtc
    23161 ctcgggtagc aggcgcgcca gttcgtgctt ccacaacgct tccagccctg cccgctcctc
    23221 cctgatttcg ggcgtccgca gccaggcaac gatgggcgcg aagggcaggt tcccctccgc
    23281 agcgaaacac tcggcgcgcg ccgcgcggat tccctgacgc cgcgcccatt gcatcaattc
    23341 cgaagccagg cgcgtcttgc cgatgcccgc ttcgcccagt atgaggacca gcgcagcttt
    23401 ccctttttgc acagaacccc acgcttcctt caaacgactc cattcctgct tccttccgac
    23461 cagccgcatc tgctccggtg ctttttcctc ccgaaactgc gcctgtgcgt tttgctccgc
    23521 accgaccagc cgctggtaaa acgactgaat ttcctgcgac ggctcgacgc cgagttcgcg
    23581 ccttatagtg gtcgcgcaag cgtaatagac ctgcagagcg cccgcgcggt cgtccatcag
    23641 cgcgcgcaaa cgcatggact ggagataggc gctctcattc aacgcgtcgg cgcggatgag
    23701 acggttcgcg gcttcgacag cttcctcgta gcggcgcacc tcttcgagtc tcttcgtcaa
    23761 ctgcgccagc gcatctaaaa acgcctgttg aaactcctcg cgtatcaaca gcacccaatc
    23821 ctgataatgc ccgggcagca actcgccgcg ataacgatta actgcctcct gcaaatggcg
    23881 gatgcgtgtc tcgtcgtcct gcacggcgcg ggtggcggcc agcgcggctc gaaactggag
    23941 cgcgtccacg ctcaggcttg aagtgtcacg ccagcggatc gtctgcccgt ccaactccaa
    24001 cagcgcgtcc gcttcgggca gggctttttt gaaatagtga atgagattgc ggagattcgt
    24061 gcgcgcttgt tcatcccccg tttcgggcca gaaaagatac gcgacctgcc tgcgcgtgag
    24121 cgccgcgtcc gcgtggagcg ccaaatacga cagcaaaagt gccatacgct ccgagcgcag
    24181 cgcttgcacc tcccgcccgt cgtatcggac gctaaacccg ccgagaagtt gaacttccaa
    24241 agttgaagag cgcatgtttt ttcgataagc acgttcgttt atgatgttta atataaccca
    24301 aaactccaca acccgcgccg tttgcggacg gggaggtttc gatgactgtc aaactaaata
    24361 aatctgttgc cgaaatggtt gggtgagtct ttttttatta actgtatacg atgtgctggc
    24421 ctccccgcat ggtgctggat aatcttcatt gacaggttcg cgcactacct gtataatacc
    24481 gtctgctttt caaccggagc aggagatctg tgaggttgtt actattccaa cttccccgcg
    24541 agctggcgac cgaagaacgc gaggtaacgg tgaagtgaag attggagaat ctttaaaaat
    24601 gaagtacgca atagtggaaa gtggcggcaa gcaataccgc gccgtcgaag gaactacgat
    24661 cgatgtcgat ttgttggatg tcgaggtagg tcacgaggtc aaactcgctg aagtgttact
    24721 ggttgtcgaa gacgaaaagg ttcaggttgg caccccatcg gtaaaaggtg cagtagtgac
    24781 cacgtccgtt gtcgaccaca tcaagggccc gaaagtggat actttcaaat actctcccaa
    24841 gaagagaatc agggttaaga gtggtcaccg ccagcgttac acccgtttaa aaatcgaatc
    24901 gatcaatttg gagtaagtga aatggcacat aaaaaaggtg gtggttcaag tcgaaacggc
    24961 cgcgatagca actcccagcg gttgggcgta aaaaaattcg ccggtgagca ggtgctctcc
    25021 ggaaatattc tcgtccgcca gcgcggcacc aggattaaac cagggctgaa cgttaaagtg
    25081 ggcaccgatg acacgctctt tgccgtagcg cacgggattg tggaatacga aaccctgccg
    25141 agcggtcaaa aacaggtcca tgtcagacca gttgaaacca agtaaaagta ttttttacaa
    25201 atccgtcaga aattgacaat tgtaaaaaac cagaatcagg aaggtcagtg ataatgaaaa
    25261 aaggtatcca tccaacatac tacccggaag cgaaggtcac ctgctcatgc ggcaatacct
    25321 ggataaccgg ttctacccga aaagaaattc gtacagaagt ctgctccaaa tgccacccat
    25381 tcttcaccgg agaacagcaa cgcattatcg atattgaagg tcaggtggac cgcttttaca
    25441 agaaactgca ggcccgtcag gactatgtcg aagagaagaa atcaaagctt gccgccaaga
    25501 cttcgcctga acgctccgtg aaggatattg gcattacggt aaaagctgca gaagcgctgg
    25561 aaaaagccgg gttgatgaat gtcggccagt tgctcgaaaa gttggctgaa ggtgaagcca
    25621 ccattctggc gattgaaggc tttgggcgca agtcccttat tgatatgaag aagaatctgc
    25681 gccagatggg gtataaattg cccgctgccg cagaagaaat tgtgatctag gtggtcaaca
    25741 aatcaactcc cctggcaaaa accaggggag tttgttatta accgcctatc ttcaaatcaa
    25801 aatgccagaa ttaaggtaga atcgttttta acccttagga ggtgaaactt ggatagaaaa
    25861 tccggccata ttgaagtgat atgcggctcg atgttctgtg ggaagaccga tgagctgata
    25921 cgccgccttc gtcgcgcaac gatagccaaa caaaaggttc aggttttcaa gccagccatt
    25981 gatgggcgct atggtttaca aaaggttact tcacatgctg gctctgaatt cggcgccaca
    26041 ccaatccgtt cttccagcga gattattccg ctgctcaaac cagaaactac ggtggtggcg
    26101 gtggacgagg ctcaattctt tgatgacgag ctcattgagg ttgtggaaga aatggtcaca
    26161 cgtggactca gggtggtaat cgccggattg gatatgaatt ttaaaggaga gcctttcggc
    26221 tgtatgcccg cattgatggc gcgtgccgag caggtggata agttgcaggc gatctgcatg
    26281 gtctgcggag aacccgccag ccgtacccag cgcctggttg atggaaaacc cgcgagatat
    26341 gatgacccgg ttgtcattat cggcgcgtct gaaatgtatg aagcccgctg ccgtaagcac
    26401 cacgaagtgc caaaagactg atcacgctgg aggtcacatg caggattatc actattacct
    26461 gagcgaagta ctgataacgg aagacgcact acagcgcagg attgccgaac tcggggggca
    26521 gatcagtcgg gattacgcgg gaaaaggcga actggtgctt gtgtgtatct tgcgcggtgg
    26581 ggtgctgttt ctcacggatt tgatgcgtaa ggtcaccatt ccgcacgcca ttgagttcat
    26641 ggccgtctct tcgtatggcg tgggcggtag agagagcaat ggtcaggtgc gcatcacgct
    26701 tgacctgaac ctggatattt ttaataagca cgtcctttta gttgaagata tcatcgacag
    26761 cggtcacact atttcagcgg tcatcgattt tttggccacc aggcggccgg ccagcatcag
    26821 cgtgtgtgtc cttctcgata aagaagagcg gcgcgaagtg gaagtaccca ttcgctatcg
    26881 cggcttcacc attccaaata agttcgtttt tgggtacgga ttggacctgg atgattacta
    26941 tcgtaatttg cctttcatcg gggtagtaga ccttcaaaaa tatcagggct ctgaacaggg
    27001 gtctgagggt gcaatttcct gattttatta aacagctcgc cagcttgacc gaaccatcca
    27061 aaccgctcta ccttgtgggt ggggcggttc gtgatgcatt actggggaaa ccttgcagtg
    27121 attttgattt tgtctgcgca caagaccccc ggccaatggc tcgggctttt gccaatagga
    27181 accacggcaa ttattttatc ctggatgaag ggcgccttat ttgccgggtc ttgatcgact
    27241 ccgcagaggt gcctgacttg atagtcgatt tcgcaatcat gcgaggggag aatattctag
    27301 aagacctcgc gcaccgtgac tttaccatca acgccatggc gattgacctc aatcagccgg
    27361 agcacatcat tgatccgttc aagggcgggc gagacttgct ggaaaaacgc ctgcgcgtgg
    27421 tttcccggga cagttttata gaagaccctt tgcgcgtcat ccgggctgtg cgttacacgg
    27481 tcgatttggg gttaaccctt gcgccagaaa taattgacct ggtccgtgct gctgtgaatg
    27541 ggcttcagcg cgtatcactt gaacgaaaac gagacgagct gtttaaaatc ttctccgggc
    27601 acaaggtgca tacggcgctg cagttgtgca tgctgtttgg aattattcct tatcttccgc
    27661 tttcaattcc gctgaatttt ccgcccctgg ttacgcgcat ccggtcactg gaagatattc
    27721 tggattggtt atgtggtgtg aaaacgcatg cgcggcagac tgccttttac caatcttcgt
    27781 tgcttgttga gctgggtcgt tttcgggaag atttgcggcg tttttaccta gcaaaaaacc
    27841 catcgaagcg tgatagaagg tcgctgcttt ttttgagtgt cattcttggc gaacaggcgg
    27901 aaccgggaga tcacgtgcga gcgctggcgc tttcggttga ggaaatgaag caggtgcgcg
    27961 attttaaggc tgccatcccg cttctgcaag gatttatcga ccgcggctct atccccgaac
    28021 cacttgagat ctatcggtat ttcaaagcca ccgcggctgg cggtgttgat gccgcggtcg
    28081 ctgcactggc ggagcaagtt tctcacgttg gctgcgaatt tgtgcaggag cagtggttgt
    28141 accagttaaa gttggccgct agactgatgg aggcctggtt taaatcgccg caggtgctga
    28201 accccgaccc cttgttgaat ggtgatgatc taatgcgccg cttctcgcta agcccaggtc
    28261 catttatcgg tgaactgctg gaattactca aggagcaaca gattaaaggt tctgtcacca
    28321 gtgcatctga agcctggcga tggattgaag gagagttgaa aaaatagagc tgggggcgtt
    28381 attgtaatgc agtttgccag gaaaccttca gcggcgaatg atccacgctc ttctcgctat
    28441 aacgcctgac caggttgttg atctgggcga tcaacacacc gcgtgaaaca ggtttgatca
    28501 aatactcatc cgctcccgca tccagcgctt tggccagggt cagcgggtca tccaataccg
    28561 aaaggatgag gatgggggct ttggtgatct tcctgatttg acgcgttgtt tccagcccgt
    28621 ctattcccgg cagcatatag tctagcaaaa caatatctgg cttgaactcc ctgaccagac
    28681 cagatccttg ttctccgtca caggcgtgct ggatttctgc cagggtggga gcgagtatca
    28741 atctgagcag gtcggttaca gaagggtcgt cgtcgatgat taatattttc ataagatacc
    28801 gcttatcaag taccataatt atcaagtcaa attgacgttt tttacctttc aatatattta
    28861 cagtaggtga ttttcagttc ttcagcaaat gaaaatcaaa tttgaccgaa taagtcaaat
    28921 aaagaaagtg cgtatggtaa aattgtcact ttggagacag aatcccgttt taacgggtta
    28981 ttttgagcct ggctcggagg aaaagatgac agataagaaa tgggtttatt tgttcaacca
    29041 gatcaaagag gttgagcagt acgtgaataa agattgggac gcagtaagag ggttactggg
    29101 cgggaaaggc gccaaccttg ctgagatggt gcggattgga ttaccagtgc caccggggtt
    29161 caccgttacc acagaagcct gtaatgcgta ccaggaaacc aagcagttcc cagaggggat
    29221 gtgggagcaa atgcttgact gcctgcaatt agtggaaaaa tcaactaaaa agaagtttgg
    29281 ctccaccaga aacccgctgc tggtctcttg ccgttctggc gcaaaattct ccatgccggg
    29341 gatgatggac acggttctaa acatcggttt aaatgatgaa accgccaagg ggatgatcaa
    29401 actcacgcag aacgagcgct ttgtatatga ttcgtaccgc cgcctgatcc agatgtttgg
    29461 gtctgtagta ctggggattc cagacgaggc attcgagaag catctggatg gtttgaaaca
    29521 ggtcaaaggc tataaattgg ataccgagat gaccgcatca gacctgactg gattgattga
    29581 ggtctataaa aaggtcattt ttgaagaaat gggcttcgag ttcccccagg atcctttaga
    29641 gcaattaaaa ctggctacgg aagcggtgtt caagtcgtgg gatgggaaaa gagcggtgga
    29701 ttatcgccgc gccacccata tcccagatga tttggggacc gccgttaaca ttcaaaccat
    29761 ggtgtttggc aatatgggtg aagactccgg caccggtgtg gcattcaccc gcgatccatc
    29821 cactggcgag aacaaactgt acggcgatta cctgctgaac gcgcaaggtg aggatgtcgt
    29881 tgccggcatc cgcaacacgg aaaagatcgc ccatctcggc agagacctgc ccgaagccaa
    29941 caaggaattc ttgcagattg ccaaaaagct ggagttgcat tacaaggaca tgcaggatgt
    30001 tgaatttacc attgagcatg ggaagctgtg gatgctgcag tgccgtaatg gcaaacgtac
    30061 tgcgctggcg acggtgaaga tcgccatcga cctggcgaat gaaggcatca tcacccgtga
    30121 agacgccgtg atgcgggtaa gccccacaga tgtggatacc ctcttgcacc cgcaattttc
    30181 cagcgagacg aagaagctgg ctgttcaaga agaccgcctg tttgctcatg gcgtgaacgc
    30241 ttctcctggc gcggcagtgg gtcaggtgta ttttgacgca gacactgctg aacggcgcgc
    30301 caaagagtac ggcgaatcca cgatcatggt gcgccccttc accaaaccgg atgatgtaca
    30361 tggaatgttg gcggccaagg gtattttaac cagcgaaggc ggggcgacct cgcacgcagc
    30421 ggtggttgcc aggcaatttg gcgtaccgtg tattgttggc gcggctgcgg tgaagatcga
    30481 tttggataaa cgtgaaatga acgcgaatgg cgttttgatt aaagaaggtg attggatttc
    30541 gattgatggt tccactggtg aagcctatat cggtaagtta aacctcacca cgccaaaatt
    30601 tgaagagcaa attgatttgc ttaatctgct cagttgggcc gaccagatct cctccaccaa
    30661 gggtattcgc catgcgccag atggctggcc aactactggt ttgcaggtgt gggccaatgc
    30721 ggataatccc agggatgcgg aacgggcgcg cacatacggc gctgtgggga tcggtctctg
    30781 tcgaactgag catatgttct ttgaaacgga acggttaccg atcgtccagc ggatgatcct
    30841 ggcgaaaacc agcgaagaac gcaccgccgc actggatgag ctgcttccct accagcgcag
    30901 agatttcgaa ggcctgtttg aagccatgaa tggttacccg gtcatcattc gcctgattga
    30961 cccgccgctt catgaatttc tgccttcggc tgaagaactg caatccaaga ttatcgcttt
    31021 acgcttgagc ggtggagggc tttcgaactt tgtgcttggc cgcggtgagc taaaagaaac
    31081 cgctgacctt gacacgatgc tcgatgccgt caatgccatg catgaaagca accccatgat
    31141 gggtatgcgc ggggtgcgcc tgagcattgc catgccagaa attgtagaaa tgcaggtgcg
    31201 cgccatcttc gaggccgcgg caaatatcgc caataaaggg atgacaccca aacctgaggt
    31261 catgataccg ctcacaggtc acgtcaatga attgacctgg atacaacccc gcctggaacg
    31321 tatcgccaaa gcggtgatgg aggagaaaaa agttaagttc gattataaat tcggcacaat
    31381 gatcgagatc ccgcgagccg cggtgacagc cggcgagatc gccagagtgg ctgagttctt
    31441 ctcgtttggc accaacgacc tcacccaaat gacttttggt tacagccgtg atgacgcgga
    31501 acgcaacttc ctcaccactt atatcgaaca ggagattctc cccaagaacc ccttccaaac
    31561 gcttgaccag gaaggcgtgg gcaagttaat gcagctcgcc gtgcaggacg gccgtttagc
    31621 gagagtgaac ctcgaggtgg gtatttgcgg cgagcatggc ggagaccctg cttccattga
    31681 tttctgtcat cgcatcggaa ataactatgt gtcgtgctca cccttcaggg tgcccatcgc
    31741 gcgcctggcg gccgcccaat ctgcgattaa acatcgcagc aagtaaccgt aaaaacgggt
    31801 tgatacaaaa cctccctgct gacagccagg gaggtttttc ttatccatct tcctgcctgt
    31861 tcatgggtgg gaatagagtg tcttgtaaat ctcgtagttc tcggtgatgt acttgatata
    31921 atccctggtt tcgtcaaagc ggatcaattc caccagcaag tcaacatcat ctccagccaa
    31981 acccttccag ataattgtgt ttccatcccc agcgttatac gacgagagcg ccggggtaat
    32041 ctcaccctga tacttgtcga tccatatttt cagaaaatgg gtacccaacc ttacgttaat
    32101 gaatggccta tcgagagatt cgacggtgta attttctggc caaccatagt tgttcacaat
    32161 cgtttcgccg gtatcgccgg tgatttgcat cagcccgtgg gctccagcgc tcgaagtgat
    32221 gctggcgtcg aagaggcttt cctggcggat aaggctgaaa atcaacaacg ggtccagccc
    32281 gtgttcaatc gccgccggta caacgaggtc ccgataaaat accccaaagc ggatgtggtt
    32341 gaaaaagatc ggtgccttgg tgagggtttc gtcttggctc aagcccgcca gatcaagtat
    32401 ctgccggctg gagagcgagg cggtttgata aaagccaagg tcaagcatgt ggttcatcaa
    32461 acggtataag ttgaccgcgt ccccttccag ttcctttcgc agttgctcaa attcgttcct
    32521 ggcgttatcg cgcaagccga gcttccagta ctcttcgccg cgctggaaca gcggattgtt
    32581 cgataggtcg ctgtacgaat gcaggtcaac cccttgatca agcgaaaaag ttgcccgcag
    32641 ccatttttct gcgtctttct tttctttatc ggtatccatc gcgatgtcat tgttgttctg
    32701 gcgagggaag gggggttcgc cccgactctt ttgcccaccg cgaataccat agtacccggt
    32761 ggggtcagcg tccgctgcct gctggtaata ggtgagcgcc tgttcgtctt tgtcctcggc
    32821 ttcaaggcat ttggcgatcc agaacctcgc cgcagcctga tcttcagggg aggttgacag
    32881 caccagcata cgctgaaaga ccaggcgcgc atcttcatag cgctgtaagc ggtagtagac
    32941 gataccggct ttgaacagag cagggtatgc ctgttcagct ccagggtatt cattgaatac
    33001 ccgctgccag gtttcagctg ccctggtcag gtagccgcca atttccaata ttttgcctgc
    33061 gagaaagagg aattctggcg tccgcgcaga atcggggtaa cgtgcggcat aatgcagcag
    33121 ggtattggtg gcgccttcgt aatcctgctg ataacgccac atcgaggaag ccttttcaag
    33181 gtaggcggta gccaggtatt ggtcgaatgg gtgctcttca atcaattcat tccaggcggc
    33241 aatttcctgg tcaatatctc ccaggcgata ataaccgtaa gccttgtagt aaagcggggt
    33301 gccatcgtga tcggggtttt ccagcatgta tctttcaagc gcgatgacgg caaggctgta
    33361 ttgccccgcg tagtaatcaa caatgccgcg catgaggtcg ttgaccggtt gtccatcttc
    33421 caccaacgtt accagcccgg aataagtatc atagtaggtt ggaaagcggg taacagaatc
    33481 ctggaaccgc gcgtaggctt gttccggcat tcccagcttc aggtaaaggc gcccgagtaa
    33541 aaggttagac tgggctttga tatagtccgc agaactgttt tccagcagcg cccagtagat
    33601 gctgatggca tcctgctctc cgccagattc agcagccgcc tgagcaattt tcagttgcag
    33661 ggtatcttcg cgaatgggct gcgcggtttt caacgcggcg agaaaagcca gtttggcagc
    33721 gccaaattcg ccggctcgca ccagcgcgtc tcccttcatt tcgaggatct cgctatccaa
    33781 tggacctggg ctggcatcca catagttctg gtacgcgtct gccgcgagga ggtactgttg
    33841 tagtctttcg taagcctgcc ccaggaagaa gtaagctgta tcgcgcgact ctccctcggc
    33901 gaaattcgtg agcagccaat tgagctggct gaccgcgccg ttgatatcgt tttgtaaaag
    33961 caacaccctg cccacaccca gctgcgccgc ctgaatcact tccgggtcgg tggattgctc
    34021 acgggcgctc caaaattcgg ctaaggcttt ctcataatcg ccatttaata tgtccgcctg
    34081 cccaagggtg atgcgcgctt ggggctgagg ggtgggtgtg atggtcgggg tcggcgaaag
    34141 ttgcggggcg gagtttgtgc cagaagggat gccgcgattt tcgcctgtgc gattgaaagg
    34201 cagcgtacag gaggtcaaca cgaacaccag gatgcttatg gaggtcaggg ctcgttttat
    34261 cataatcctc ttataccgga ggttt
//
LOCUS       DAOSPM010000006        33522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1348_length_33522_cov_11.044671, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000006 DAOSPM010000000
VERSION     DAOSPM010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 33522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33522
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1348_length_33522_cov_11.044671"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="PLW25_01310"
     CDS             <1..1146
                     /locus_tag="PLW25_01310"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOS53156.1"
                     /translation="TPETNIVDLGLIAPDGRQVGASGSEKTRILVSEQYATPGYGART
                     VSAGRWQVIVGAYQVAPEGVEVLYEITLKEKSLRLLKGDTHVHSIASDGVHTFEELAL
                     KAKANGLDFLAVTDHNVFLPAASIPYVPGITMIPGVEWTHYQGHANFLGIDAPYDEPF
                     FTNSVEEARSRFTCAHERGALITINHPFEGNSRFLFDFRQLPFDCLEVWNGPMRESNL
                     KAVGLWQQMLSSGMRVAITGGSDYHRDTPFIFLGGPTMGVYALSAGMSDILSALRKGH
                     SYITFAPDGPSIEMRVGEGIMGDQVDWQEEKELRVRLQGLQAGDVVLVVTAQENAAVF
                     TATSEGELDFAYEMREPGFARVEVLRAFLPGLPLLPALMSNPIYFKA"
     gene            complement(1136..2284)
                     /locus_tag="PLW25_01315"
     CDS             complement(1136..2284)
                     /locus_tag="PLW25_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53157.1"
                     /translation="MKSGFSLRNWTEKVEALPSWVLLVVASVLYIPLVFFGYGSDSDS
                     LGVVCTGQYFIATFDYLPSRLPGFFVHEFFVFFLNQLGGSVLTNLGSVCISLVMLHSF
                     RQVCRHYAVPHPTLLSSVLMVQPFFWVSAASTIDYLWALGFCFLGFNLLLHQRNLPAA
                     ISLALSVGCRLSTVLPVGLFLCFLFLTSVPQRRWILLTSFCAALMSIVFFLPPLDFLE
                     WDFRRWLVLSTGDPALWTPVLRVGRFLYKNLMFFSLPVVLWACFLCLSALFHKQKPTL
                     PRPDSLSWLSLAVILSVEIMFLRIPIEMEYLLPLLPFALILIGKALPNHPGQLWLLLA
                     LTLLANFLWINPARSLTPNQTSEVIYGLWLEPGYLLQDIATRLSLLKP"
     gene            complement(2332..3456)
                     /gene="alr"
                     /locus_tag="PLW25_01320"
     CDS             complement(2332..3456)
                     /gene="alr"
                     /locus_tag="PLW25_01320"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOS53158.1"
                     /translation="MLYQTHAQIHLANIRHNIEGIRHAIGQQCKILIAVKANAYGHGA
                     VEVSRMAQNIGVNWLGVATVPEGMELREAGIHLPILKFSPAFPEEMECAVENELTLTV
                     CDKANIQALQRVCQRLKLTARVHLKIDSGMGRIGVTPGEAPELAKFIHSRCSNLLLEG
                     VFTHLPVSDSMGNDYTQAQIDRFKSVVLTIEDAIGAKVPLVHCANSGGVLDYPQSWMD
                     MVRPGIMIYGFYPGEPSSHRVDLKPGLSLRTRVSFIKKVEKGTSIGYGRTWFAPQDTW
                     IATIPAGYADGFNRLFSNTGRVLIDGVSYPVVGRVCMDQSMINLGAETHVKVGDEVVL
                     IGRSGAEEISAYEWAEKLNTITYEVTCQINQRVPRVFDLY"
     gene            complement(3468..4559)
                     /locus_tag="PLW25_01325"
     CDS             complement(3468..4559)
                     /locus_tag="PLW25_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HOS53159.1"
                     /translation="MSKLFTPRLELFPERIAENARAIIHLCHDHGIQVACVGKVVCAH
                     PVIMRALEESGADLLADSRLENLRSLRQNGTTLPLMLLRIPTPSQVEEVIHAADISLV
                     SDLETIKKISAAAMAAHSTHKVILMVDLGDLREGIWPSRLDEVLRAIIRLPGIEVNGL
                     GCNLACYGGVIPTTEKMQQLIDLRDQSETSLGIRLPVLCAGNSANLPLLLADKMPLQI
                     NMLRIGETILLGRNVLDRSPFPGTRQDTARLVAEIIELERKPSVPIGERGQDAFGGTP
                     EFVDRGVRLRAICNIGRQDVVIDNLTPEDPGIIVLGGSSDHLLLDVQDAQQPLAVGDE
                     VAFYPGYGALLAASTSPYVRKIMINGISS"
     gene            complement(4556..5893)
                     /locus_tag="PLW25_01330"
     CDS             complement(4556..5893)
                     /locus_tag="PLW25_01330"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOS53160.1"
                     /translation="MPEPICILTIEVGSTITKVNGFYRSQHGALVQAAQGFAPTSITQ
                     GDVMPGVELAMQQVKDGANPLDPEVEIFINSSAAGGLRMTVHGLTYNMTARAAREAAL
                     GAGAIVKKVTAGLLDEFELSEIRQIHPNIILLAGGVDYGEKETVLENARRIANLGLQV
                     PVVYAGNRVLRQPITEIFKTQDIDLLIADNVFPEVDVLNIEPLRRIIHEVFNRHIIHA
                     PGMARLGELTRHGILPTPGAVLLASELFAEEVGDCVVFDVGGATTDVHSVTDGSQEWS
                     LKLLEPEPRAKRTVEGDLGVYVNAKHIIEIVQDWESEDRLADLQAIPVTDKQLELTHW
                     LCTKAVEISARRHAAQVTDLYTPSGRKQVVKGKDLTAVKRVIGTGGALTRVTGGVEIL
                     QRVCTGAGKYLLPQPGTPILLDDAYRFSALGTIAQKYPDEVKSTFVDWVRKEI"
     gene            complement(5915..8128)
                     /gene="oraE"
                     /locus_tag="PLW25_01335"
     CDS             complement(5915..8128)
                     /gene="oraE"
                     /locus_tag="PLW25_01335"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF040743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HOS53161.1"
                     /translation="MTNTPEKLDPNQKMDIEHILEGLEHYHPRRKGWTWREIPQEGVE
                     MGPFHYHDMSKPLKNSIGLPASKYFDHIDPQPSCVVTTEIASGRFEDDIRRMRMAAWH
                     GADHLMVIRTTGQSHIDGLMEGTPEGVGGVPITRKQIRASRKACDQIEEEVGRPINFH
                     SYVSGVAGPEVAVLFAEEGINGAHQDPQYNVLYRNVNMYRSFVDAAEAKKVMNGARIF
                     QIDGAHNANATAKAGWLVMPELMVQHGINCSFSVAVGMDKDLIGLSTVPPNAPPAPKL
                     WYDLPYAVALRDFFSDFKMRAQQNTRYIEADIEEAIRTHTIDTLISMLTSADIQSTIT
                     PDEGRNVPWHYNNIRGVQTVKQTWAALDGIKDLVALKTNGPMKEMVRDLKVRAVAFLE
                     EVIETGGYFAAVEKGFFVDSAKYPERNGDGIARDGKGGVAADTIVERDADYFAPVCDH
                     FGYNNVPAELQRPCDAIQGCTLCKPEKIVYIDELDPEDNCEKRLQETYPYRKGGKIKP
                     EAEWAGDGTILTQFFVPLPEDYARVYGVEMAKKLGLIDPEVINIQVMHPAEGCFVEVK
                     GKVNFELNLTDLKIPAKEIMLEEETLRQVVKKVGLTVVAGTVGEDEHSVGLREILDIK
                     HGGIEKYGVKYHYLGTSVPVSKLVDAAIETGAQAILISTIISHNDVHRSQMKKLADLC
                     VERGVRDRLILIAGGTQVNAEMAAETGLDATFGRGTKGIHVVNAMVKNLQKRGLA"
     gene            complement(8140..8490)
                     /locus_tag="PLW25_01340"
     CDS             complement(8140..8490)
                     /locus_tag="PLW25_01340"
                     /inference="COORDINATES: protein motif:HMM:NF027866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HOS53162.1"
                     /translation="MSEDRIKRFTERQKELNKLSDEELKNQFWQLCDRLVEPIVELAR
                     THTSPSIERSVLLRMGIDSITAHGVVDRVLEAGLLGKGAGHVVLKVAEKQGVSIPTAA
                     ALINEKKEILSGLF"
     gene            complement(8483..9928)
                     /gene="ortB"
                     /locus_tag="PLW25_01345"
     CDS             complement(8483..9928)
                     /gene="ortB"
                     /locus_tag="PLW25_01345"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: protein motif:HMM:NF040741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HOS53163.1"
                     /translation="MSQRDVSYAAVMARKNDIMKSSMGMDYEKYISSPVAFDYEAMMR
                     DTGYTMDEIFRIQQETKVGNTPLYELRNLTEAVRQISPPGKGATILVKDEAANASGSF
                     KARRASISAYEAAHKGYAGMVAATSGNYGAAVASQAAQRKLKCIIVQEVYDSRGIGQP
                     EIVEKGRACEAYGAEVLKLTVGPELFYMLLRTLEETGFFNASLYTPFGIRGVESLGVE
                     IAEQTRARYHRDPDAVIITHAGGGNLTGTARGLLHAGSCATRIVACSVNLQGLHMASD
                     HDFNRKSFTTGHTGFGVPFATWPDRVDVPRNAARSLRYMDEYQLVTQGEVFYITELLT
                     KIEGLERGPAGNISLTAAVALARQMEREQVVVVQETEYTGAGKHQNSQLAFARENGIE
                     IRRGDPLDDIPGKTIIIPEKLEQVQGKSQDIDRLRLEYLQNAGKQLAPSLWSESDISF
                     LALDIKKTPDWIRENFLSLPQNNENLEKTNV"
     gene            complement(9925..10209)
                     /gene="ortA"
                     /locus_tag="PLW25_01350"
     CDS             complement(9925..10209)
                     /gene="ortA"
                     /locus_tag="PLW25_01350"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: protein motif:HMM:NF040739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HOS53164.1"
                     /translation="MNIIPKGTWVEIEQVVLKPEERAASLPEDTKKVPYLLWVSGFLL
                     EDAKIGQKVRVKTIIGRELSGELKVVNPSYHHSFGTVVPELLTIGTEYEK"
     gene            complement(10241..11281)
                     /gene="ord"
                     /locus_tag="PLW25_01355"
     CDS             complement(10241..11281)
                     /gene="ord"
                     /locus_tag="PLW25_01355"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF040740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HOS53165.1"
                     /translation="MKPIRVLQWGLGAMGSGMTRLILEKSGLQIVAAVDSRTDYAGKD
                     LGDVLKIDRSLGVTVTSNPDSVLNKERVDIVVIATTSWLSEQMHDLKKIIQAGINCIS
                     IAEEMSDPYAQNPELAAQLDVLCKANQVTLLGTGVNPGFVLDLLVVMLSGGSHTVERI
                     EASRVNDLSPYGPTVMKSQGVGITPAAFKAGIEDGSIVGHVGFPESIHMISDALGLGV
                     DRIEQAREPIISNVYRETPHVKVQPGMVAGCAHTGIGYRGDKEVIRLIHPQQIHPQLE
                     GQDTGDYIHIFGKPEINMAIKPEIAGGIATMGLAVNMIPHVVAATAGLKRMIDLPAPA
                     ALMGETAYRRQV"
     gene            complement(11394..12509)
                     /locus_tag="PLW25_01360"
     CDS             complement(11394..12509)
                     /locus_tag="PLW25_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOS53166.1"
                     /translation="MILAQELKALNLTILEPEPATAASKNRVLPDDPGGWLLSGSLED
                     VYRSNLYLRTASRVSVRLGEFYAAAFSELRKKASRLEWERYLSPGQPVHLSVTCHKSR
                     LYHSDAVAERVVGAINDRFSNSKKETQPSKNGQLVLVRLVNDLCTISIDSSGALLHKR
                     GYRLESTKAPLRETLAAALLLLSGWSGEMTLIDPFCGSGTIPIEAALLARNIPPGVAR
                     DFAFMHWPGFVPHEWERVLHSARDGIIRQDSHIFGYDRDQGAVQSAISNASRAGLNEV
                     IHFQRQSISELSPPRSNPGWLITNPPYGVRISGSKDLRNLYARFGNLLTTAFSQWQAG
                     ILCYDKKLTANLGLPPQQASYHFTNGGLPVDFNVFAF"
     gene            complement(12563..14401)
                     /locus_tag="PLW25_01365"
     CDS             complement(12563..14401)
                     /locus_tag="PLW25_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOS53167.1"
                     /translation="MIKAAYRLLFFTSRLRCYTQFVRQQGTIVFIPTTREELVHLGWE
                     QPDVILVSGDSYIDTPYSGLAVIGKILISQGYRVAIIAQPDMSGSEDIRRLGEPLLFW
                     GVSAGTVDSMVANYTATLRKRRTDDFTPGGANIRRPDRAVIAYTNLIKRYHKNTVPIV
                     LGGIEASLRRITHYDYWSDRLRAPILFDSKADYLLYGMADGSIVELALALRQKRSPRE
                     IRGLCYLAAEAPSTYLELPSFEEVSADPDAFIHMFDLFYRNNDPLTAQGLVQKKGDRY
                     LVQNPPPPTPSTQALDTIYDLGFERLHHPYYELQGEVKALQTIQFSIPTHRGCYGECN
                     FCAIAVHEGRTVVSRSEASILKEAEKLSHHPDFKGMITDLSGPTANMYGFECRKKLVS
                     GACEDKRCVFPNICPALPVDHSRQVALLRKLRNLPGVKKVSVGSGIRYDLVLADRRSG
                     MQYLKDITCYHTSGQLKVAPEHTDPVVLNLMGKPSHESLRLFREKFLEFSRQAGKKQF
                     LSYYLIAAYPGSSEREMKKLREFVTKELRVQPEQVQIFTPTPSTYASVMYYTEKDPFS
                     GKKLFVEKNLHRKALQKQIITNPGRSKTGGTQKPFEKHPLSRNRKP"
     gene            complement(14398..14910)
                     /locus_tag="PLW25_01370"
     CDS             complement(14398..14910)
                     /locus_tag="PLW25_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOS53168.1"
                     /translation="MNPSNDLRKVSKDLVVSMAYELKIEGELIDQATEDDPLQFIQGH
                     GHIITGLESAIEGMNIGESKHVSVKPEDGYGVYSEEDIIEIPRSEFPEDFPLEVDMEV
                     TVEDDEGIESSAFIEEITADSVTLDFNHPLAGRDLEFEVKIIALRLPTSEELNHDHVH
                     LDEHEQLEDE"
     gene            complement(15011..15403)
                     /locus_tag="PLW25_01375"
     CDS             complement(15011..15403)
                     /locus_tag="PLW25_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53169.1"
                     /translation="MTRDAKIPDRVIRKIERKFHIKTNGYFLVDNFSASSIETWLGLE
                     NPSSPSQPPLTPLERQTILSHGQKVISQFCAQISQNGIDQVINQIHWADLLPEHFSTN
                     VSTELWLQAVNKLASFEKIGSCEVFIGQ"
     gene            complement(15400..15708)
                     /locus_tag="PLW25_01380"
     CDS             complement(15400..15708)
                     /locus_tag="PLW25_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53170.1"
                     /translation="MKKIALALIALLFLVSVYFGFQAAAHLDGHLKKTAPNNSLEPIS
                     AQQNYLIFQVSELGGDSPMLVSVWGLFVRDAPPLTWRLSRSTHPQIQSKKTTHSPFSV
                     "
     gene            complement(15803..16021)
                     /locus_tag="PLW25_01385"
     CDS             complement(15803..16021)
                     /locus_tag="PLW25_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53171.1"
                     /translation="MIAEIINSIELSFWNLIITLLTKSSLLRTIVRSTYLASQDKGMM
                     RKIAIVLLVFCVGFASGLLIYSVTLLVM"
     gene            complement(16030..16248)
                     /locus_tag="PLW25_01390"
     CDS             complement(16030..16248)
                     /locus_tag="PLW25_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53172.1"
                     /translation="MDARIKNAVCTSVYRQFPELKGVPPRVKALPADKYQLIFHGQAK
                     TADGKTLSRTVRVVSDENGKILKLTTSR"
     gene            complement(16259..17425)
                     /gene="ftsZ"
                     /locus_tag="PLW25_01395"
     CDS             complement(16259..17425)
                     /gene="ftsZ"
                     /locus_tag="PLW25_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOS53173.1"
                     /translation="MDYSTFHSHVARTPIKANPPVLKVIGLGGGGQNAVDRMIELGLE
                     GVQFIAANSDAQVLQHSQADLKIQLGRKITRGLGAGGDPAVGQAAAEESIAELTSALT
                     GADMVFLTAGMGGGTGTGAIPVAARVAKSVGAVVVAVVSLPFSFEIGRRQTNARNGLL
                     MLQQHTDTLITVPNDRLLQVAPRNLPIEMAFRLADDILRQGIQGISELITQPGLINVD
                     FSHIKNMMLNGGGSLLAIGTGKGENRVSDALEHALHHPMLESIDLEDASGIIANFTGG
                     NDLPFFEVTEALNTLQEKSGGMAEIVPGIICDDRMEDRVQVILVLTGIGTTALDPQRM
                     KRPVPVMTPAEDTDKETSLSPGNSYQENIPDVHPELAAAQSELDVPAFMRRKAR"
     gene            complement(17428..18771)
                     /locus_tag="PLW25_01400"
     CDS             complement(17428..18771)
                     /locus_tag="PLW25_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53174.1"
                     /translation="MILKSKKTRELVTGRVGIHYFPDTSHYTNKDLSNWLPVLQRLKC
                     AWLVLLSDTSRAIPEQFITALIKAGITPLVHIPLPLPNTPSPADLKAILAAYARWGVK
                     HIILFDRPNDMANWSAAGWVQQNLVERFVDRFLPLATATVKAEMMPIFPPLQPGGSYW
                     DLSFLKQSLQSIQRRGHSSLINQMGLASYAFTFNHELDYGAGGPQKWPKSLPYITDAQ
                     GQDQRGFFNFEWLQATARTVCDQDLPIFMLGAGIREPGQPYSPEVHAGICLSIMEMLK
                     ENSSKTAIPDYVISCSFYLLSAEPDSEEFACAWYKSPEDYLPVADLLMQAQVPGASNH
                     ANVPLEKADLLVEAESTPIKSFTGHPIDHYLLIPVYEWGVADFHLDVTRAFVQKHRPT
                     IGFSIREAALARRVTVIGGEQSFPETSLEELRRSGSVVERISGDGTSIATQLAER"
     gene            18871..>19319
                     /locus_tag="PLW25_01405"
     CDS             18871..>19319
                     /locus_tag="PLW25_01405"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOS53175.1"
                     /translation="MILVTGGTGFIGRSLIQALVSSGEQVRVLLRPSKTSPNFPKGIS
                     VEVAVSSFSDQRSLRAALKGVDFLYHFAGAERQGLNANLNQIDVEGTATLMRAAKDTN
                     LKRVFYLSHHGADRGSAYPVMKAKGIAENWIMDSGIPYTILRTGAVFG"
     assembly_gap    19320..19419
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19420..19632
                     /locus_tag="PLW25_01410"
     CDS             <19420..19632
                     /locus_tag="PLW25_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53176.1"
                     /translation="IQRKPLGVTPEFLRGLTLWIAQVYPKLPVSIFWLDYLAVDRITS
                     LDSVPREFGLIPARFKNKIDYLNKKI"
     gene            19639..21057
                     /locus_tag="PLW25_01415"
     CDS             19639..21057
                     /locus_tag="PLW25_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOS53177.1"
                     /translation="MPENRAFCIHAHFYQPPREDPFTGKIPEEAGAAPFANWNERILA
                     TCYQPNVEERNFSQLSFNLGPTLVEWMSQHAPDVLDRIVQEDALNATLYGTGNAIAQP
                     YHHTILPLASNEDKRTQVLWGIAAFNHVFGRAPLGMWLPETAVDYESLRVLASAGIQF
                     TMLAPWQVEVIEGESPYLVELGAGKSIIVFVYHGGLSSTVSFDAFATSNADAFAEFYL
                     KPEIDRFNPNQFLLIATDGELYGHHQPYREKFLSRLLDGSSNGIGLRATYPALWMKNH
                     QVTGRARIVENTSWSCFHGIDRWRTECDCTAGSTWKAGLREGLSLIAARIDDAFSEIC
                     AAHGLDPITARNDYIQVMLGMDTFEQWLSKTSGKEVSSIGKRKLATLFSAQRLRQQMF
                     SSCGWFFDELARIEPRNNLAYAAHAVVLMESLTSMDFHSVAQPALREAVSHTSGETAF
                     DFFIFAYQRFKNLQKTAQANVG"
     gene            complement(21017..>24378)
                     /locus_tag="PLW25_01420"
     CDS             complement(21017..>24378)
                     /locus_tag="PLW25_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOS53178.1"
                     /translation="DLVTFPEQAAKASQVNAMGLIDEIFHHIFKLYKEQKNPQVLKQA
                     LAYLEQQSSSAALNAFLELFIREFPPSPVYQKQVGAHEYLLGNTDGVSNRELLIEELI
                     MLWLSLENPALDSYRELFAEKTFINDSRYALFISEINHFFALQPYFGPSNFPLTNMLR
                     SPAIAVPDSITGQLEYIREHWAMLLGDYLYRLLSSLDLIKEENKLGITGPGVTAIPVY
                     DRDGFFQAGGSSEIEAFSKDREWMPRLVLMAKNSYVWLDQLSKKYQREINRLDQIPLD
                     ELETLSRWGITGLWLIGLWERSPASARIKKLCGNPEAISSAYSLHSYQIAADLGGENA
                     YLALKENAARFGIRLASDMVPNHMGIDSDWVIHHPDRFLSLDYSPYPSYSFNGENLSS
                     HPDVRIQIEDHYYDRTDAAVVFKRQDLRNGQVDYVYHGNDGTSMPWNDTAQLNYLNPE
                     VREAVIQTILEVAHRFPIIRFDAAMTLAKKHYQRLWFPVPGSGGAIPSRSEHSLTAEE
                     FNRLFPVEFWREVVDRVAVEAPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNMLRN
                     EDNAGYRRLMRNTLEFDPEILKRYVNFMNNPDERTAVEQFGKSDKYFGICMLMATMPG
                     LPMLGHGQIEGFSEKYGMEYRKAYWDEPVDNDLVSRHEREIFPLLHQRRLFAGVDHFN
                     LYDFFTNNGNVNENVYAYTNYDAGNAALIVFNNRYEDTEGWVHTSVPQLRCYGQEKKL
                     EQVSIAQALHLPADRNGWLVFRDQISQLQYLRPLDEVLDRGFFFRLHAYHYQTLLDFQ
                     VVHADSAHDYASLYGLIGRQGVPDIAQALNELTLKPVLEPFQVIVNQGYLGYLLQKYS
                     TSPTLDSTEAREIQERIGRFLQGVALYTGNSGELRSLQQETSSLFLTALMFSQELKKR
                     VTRLSPSTSTFLDALENHLEQSPALRVTLLMLSLTSRLGKVASQEDFSEQSLAWLDEF
                     RLNKTFIEVLQSMGVTSQEIQNSALLLRIGITHQGWYNATKNEPMEEIIVRWFTDPQV
                     QTLLRVNRYQDVLWYNRESFETLVWWLSLIALYQYIANPGADASTITEVVLNLNQLLG
                     KLKKLEAKSGYQFDRLFDFNQHSPGQSSVNS"
     assembly_gap    24379..24478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24479..24813)
                     /locus_tag="PLW25_01425"
     CDS             complement(<24479..24813)
                     /locus_tag="PLW25_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53179.1"
                     /translation="MAIVSASRLTDYRALTFRFSPGFSLSTPEQAVEYVKERGFIFFW
                     PVKGFPFPSLWGAVAGDRPVTDEHDDPGHITWHWKDSLLDQRVWYYARVLRRKNTLIS
                     LDALKYFYAL"
     gene            complement(24873..26033)
                     /locus_tag="PLW25_01430"
     CDS             complement(24873..26033)
                     /locus_tag="PLW25_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOS53180.1"
                     /translation="MRQVAVIGIGQTKIDEHWDKSLRELAGDAALAALKDSGLERVEA
                     VYVGNMMSGSANHQQHLGAYLADWIGMRGAEAIRTEAACSSGAASFRSAVMAVASGLV
                     DSALAVGVEKMTDSPLNEITAALATAADAEWEAGNGLSFVSLNALLMKRYMYEYGWEK
                     EHFAPFAVNAHANAMHNPYARFHEPVTVEGYLKAPMITDPINLMDASPTGDGAAAALV
                     VPLEKFHQNKGRKVIRVAASSAATDTMAVHHRKDALFLKAAYNAATKAYAQSSLTPQD
                     IDLFELHDAFSIMATLSLEANGFAEKGKGPQLALDGEILTNGRIPIATRGGLKARGHP
                     VGATGMYQIVEVVEQLRGEAAGTQVENARVGMTQNIGGSGSNIISHIFAVTD"
     gene            26164..27837
                     /locus_tag="PLW25_01435"
     CDS             26164..27837
                     /locus_tag="PLW25_01435"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOS53181.1"
                     /translation="MHIAQRLKISLVLFFFVLCASCAGIPTQSQEITSTPTFFQSAPL
                     QPTPIPEEPVLFVSNGVPEDWLKKIDGFPHISKTEQKEQANLFFTVHIGKFIEPQVFS
                     STRVYAVVEPFFTIRDEISSNELKDLWSGKAVEGQHQTLVVTEETAQVIGAVWGKPAG
                     EVQIIPSDGLLAYCEEHPEALAILPFEDLSPRWKVLRLDGLSPLDKFLAVDRYPLTIH
                     YTLQSHLSNPSMVSEIVELFSDALPLTNRDESKMTVLMMTGTTAITRVLAYKISIKGM
                     EYPIAAVQDWFVHADLKHVSNESPLVEGCPVPDEFSSSLVFCTPPDMIEVLELLGVNI
                     IELTGNHINDHGPENLEYTLKAYRDRGWHVYAAGNTVEEARSPLLIESNGNKVAFIGC
                     NAVGPATVFAKEGRAGAAECDFEYLFSEIRELKQKGYIVVTTYQHEEEENLMYFEKIS
                     KDFQLAAEAGADIVQGSQAHSPMGFEFVGDHLIHYGLGNFLFDQMVEWNRREFIDRHI
                     LYNGKYINTELLTATLVDWSRPTPMTEEERTQLLKEIFTVSLKRKNENK"
     gene            27824..28459
                     /locus_tag="PLW25_01440"
     CDS             27824..28459
                     /locus_tag="PLW25_01440"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOS53182.1"
                     /translation="MKINDVTLTIREGMVVWPGEPEVDLHRIKKLEEGANANVTLMGF
                     AVHTGTHVDAPRHFIADGNSVDLLDLNMLIGEVTVVEIPQDVDHISAAVIARSGLAVG
                     TERVLFKTRNSNYWVENRGDFQPGFVGITEDGAQALVELGVKLVGIDYLSIAPYKMSR
                     PTHLVLLKEKMVVIEGLDLSKVKPGKYTLYCLPLKLANADGAPARVILIED"
     gene            complement(28470..28856)
                     /locus_tag="PLW25_01445"
     CDS             complement(28470..28856)
                     /locus_tag="PLW25_01445"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HOS53183.1"
                     /translation="MPPGDLTFPREVDLGINLINQGDYFQAHEVLELAWRNEQKKQRQ
                     LYQGLVQVSVLLYHLARGNTKGALKLLKKALLNLTPFVGAATTLDIPRLVSDLNDLRA
                     RIESGQHESGSSLTDRFTLRYRQPED"
     gene            28950..30299
                     /locus_tag="PLW25_01450"
     CDS             28950..30299
                     /locus_tag="PLW25_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS53184.1"
                     /translation="MIEMADADILPYSLEHNFWTWSAQAKVKPIPIKSAKGVYFWDVN
                     GKKYLDLNSMVMCANIGHGDERVIQAMIDQARELPFAGPQFATKPRAMLGKKLAEILP
                     EGLTKYLYTLGGADANENAVKLARAYTGRHKILTRYRSYHGATFGAAALTGDPRRMAW
                     EPAVMPGVVHFLDPFRYRSTFHQRYPDVDEDEFSRDYLGHLEEIIQYEGPQTIAAILI
                     ESVTGTNGIIVPPEGYIQGVRDICNRHHILMIADEVMSGFGRTGEWFAVNHWGVVPDI
                     MTMAKGLTSGYAPLGVVAMKPEIAAAFNEKVYEGGLTYNGHPISLAAAIAVIEVMQQD
                     RLVEKAKLSGEILKNMLQNLKARHPSVGDVRSIGLFAAIEVVKNQQTKEPAAPFGGSS
                     PEMSALRQYLLDHGVYLYTHWNTILIIPPLIITQSELEQGLSVIDQSLYEMDKVVNH"
     gene            30299..>31063
                     /locus_tag="PLW25_01455"
     CDS             30299..>31063
                     /locus_tag="PLW25_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS53185.1"
                     /translation="MEKMKEERKIESVVILGAGPAGLTAALYAARAELTPLVITGMQL
                     GGQASLTHQIENYPGFPEGIGGADLGDLFKQQAERFGARLAFDVVTEVDLSQLPFRIK
                     TYGQEIFSKTVIVATGADPVHLSVPGEKELTGKGVSYCATCDGYFFKNKHVVVVGGGD
                     SALEEAIFLTKFADKVSLIHRRDTFRAGAILQSRVRENHKIECIMDSVVKEIKGIDKV
                     NAVIIQHVKTAALTELPADGVFIFIGHAPNNGIYQGQ"
     assembly_gap    31064..31163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31164..32033)
                     /locus_tag="PLW25_01460"
     CDS             complement(<31164..32033)
                     /locus_tag="PLW25_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOS53186.1"
                     /translation="MTPENVSSTSKTCPTCGSRLSENATRCLVCGRTFTPAATKKTET
                     SPVQNPKLPELKVSLPAAIALIVLILGLGAALIFVILQTTGRVTTPTATPTVTNTPTL
                     TLTPTATLEPTLGPTPTPVPPREYVVKEGDLCSSIALSFGVSIQSIASLNGLPPDCGI
                     LYPNTTLKIPWPTPTPTALPTATLSAAEATQQACGTVPYQVTENDTLSSISANYNISI
                     ESIKTFNGLTSDTVWVGMNLSLPLCERMPTQGPTPTATLLPPYPAASLLLPEDGAVFT
                     NVNESITLQWSSVG"
     gene            32129..33337
                     /gene="hemW"
                     /locus_tag="PLW25_01465"
     CDS             32129..33337
                     /gene="hemW"
                     /locus_tag="PLW25_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOS53187.1"
                     /translation="MEISIYFHIPFCNRRCDYCDFNTYAGLERQIPEYVKALNRELEM
                     VLKNAPTDFKVTTIYFGGGTPSLLNSTDYATLFTTIGNSAVVSPDAEISLEANPDSLS
                     DEKITAYRQVGFNRISIGMQSASRFDLKALGRAHTNQSLLASVDGCRRAGFQHINLDL
                     IFGIPGQTLESWQRTLRLALELGADHLSLYSLVIEEGTRLRRLFDKGLLVEVNDDLAA
                     DMYETAMEELAQAGYSQYEISNWAKNSDARCRHNLQYWRYAPYLGFGAGAHGFWGSTR
                     TENLYTIQEYMQAVNLKSPAAFPAGCACQSTITMNRWEMMQENLMVSLRLTEEGVSLS
                     RFAERYGVELMEVFPHQLEKTLREGLIEYDEENDRLRLTYRGRLFGNRVFSQFVSNKV
                     PKGYEHLLAQ"
     gene            complement(33331..>33522)
                     /locus_tag="PLW25_01470"
     CDS             complement(33331..>33522)
                     /locus_tag="PLW25_01470"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOS53188.1"
                     /translation="FFEPENGIAFHGTYWHQNFGMPMSHGCINMKTEEARWLYRWVTP
                     EGDMSKMTTIGFGTQVHVY"
BASE COUNT         8094 a         8156 c         8861 g         8111 t
ORIGIN      
        1 actcctgaaa cgaatatcgt ggatcttgga ttgatcgcgc cagacggacg gcaggtgggg
       61 gcttctggct ctgaaaaaac caggatcttg gtgagcgagc agtacgccac tcccgggtac
      121 ggcgcgcgta ccgtttcagc ggggcgatgg caggttattg ttggtgcata tcaggttgcg
      181 ccagaaggag ttgaggtgct ttatgagatc acgctcaaag aaaaatcgct gcgcctgctc
      241 aaaggagata cgcatgtgca ctcgatcgct tcagacggcg tacacacgtt tgaagagctg
      301 gcgctgaaag ccaaggctaa tgggttagat tttttggcgg tcactgacca caatgtgttt
      361 ctgcccgctg cgtccattcc gtatgttccc gggataacga tgattcctgg cgtggagtgg
      421 acccattacc agggtcacgc caattttctg ggcatcgacg cgccctatga tgaaccgttc
      481 tttacaaatt cagtggaaga agccaggtcg cgcttcacct gtgcccatga gcgcggcgcg
      541 ctgatcacca tcaaccatcc cttcgaaggc aacagccgtt tcttgtttga tttccgccag
      601 ctgccttttg attgcctgga ggtgtggaac gggccgatgc gggaatccaa cctgaaagca
      661 gttggcttgt ggcaacagat gctcagcagt ggtatgcgcg tggccatcac tggcggcagc
      721 gattatcacc gcgacacacc gtttattttc ttgggcgggc cgacgatggg tgtatatgcc
      781 ctgtctgctg ggatgagcga tattctgagc gcgctgcgga aggggcatag ttacatcacc
      841 ttcgcgcctg atggaccatc catcgagatg cgcgtgggag aagggattat gggcgaccag
      901 gttgactggc aggaggaaaa ggagctgcgc gtacgcctgc aagggctaca ggcgggagat
      961 gtggtgctcg tggtcaccgc gcaggaaaac gcggcggtgt ttaccgccac atcagagggc
     1021 gagctggatt ttgcctacga gatgagggaa ccgggttttg cccgcgtgga ggtgctgcgt
     1081 gccttccttc caggcttgcc gttattgcca gcgctgatga gcaacccgat ttattttaag
     1141 gcttgagcaa agacaatcga gtggcgatat cctgcaacag gtaacctggc tccagccaca
     1201 atccatagat gacctcgctg gtctggttgg gggtgagcga acgcgccggg ttgatccaaa
     1261 ggaagttcgc caacagggta agggccaaca gcagccagag ttgccctgga tggttgggca
     1321 atgccttgcc aatcagaatt aatgcaaaag gtaacagggg gagaagatat tccatttcga
     1381 tgggaatgcg caggaacatg atctccacgc ttaagataac cgctaaagaa agccagctca
     1441 aactgtctgg gcggggcaaa gttggttttt gcttgtgaaa caaggcggac aggcatagaa
     1501 aacatgccca aaggacgacc ggaaggctga aaaacatcag gttcttgtaa agaaagcgtc
     1561 ccacacgcag cacaggcgtc cacagagctg gatcaccggt gctcaacacc agccagcgac
     1621 gaaagtccca ttccagaaag tccaatgggg ggaggaaaaa aacgatcgac attaacgcgg
     1681 cgcaaaaaga ggtgagcagg atccagcggc gttgtggcac actggttaga aaaagaaaac
     1741 acaggaacag tcccacaggt aagacggtgg ataaacggca gcctacagac agcgccagtg
     1801 aaattgcagc aggtaaatta cgttggtgaa gcaacagatt gaaaccgagg aagcagaagc
     1861 ccaaagccca aagataatcg atggtgctgg cagcgctcac ccagaagaaa ggctgcacca
     1921 tgagcacaga gctgagcaaa gtggggtggg gaacggcgta atgacggcat acttgccgga
     1981 aagaatgaag catcacaaga gatatgcaaa cagaacccag gttcgtcagc acgctgccgc
     2041 ctaattgatt taagaaaaac acgaaaaact cgtgcacaaa aaagcctggc agacgcgagg
     2101 gcaggtaatc aaaagtggcg atgaaatatt ggccggtgca caccactcca agagaatcgc
     2161 tatcgctgcc gtaaccaaag aaaaccaatg gtatgtaaag cactgaagcc actaccagca
     2221 atacccagga aggcaacgcc tctacttttt cagtccagtt acgaagagaa aacccggatt
     2281 tcattgtttt cgtcacatcc tgttcggctt tggcagtgag gggaattaag gttaatagag
     2341 gtcaaaaacg cgcggcacac gctgattgat ttggcaggta acttcgtaag taatggtgtt
     2401 gagcttttca gcccactcgt aagcgctgat ctcttcagct ccgctgcgac cgatgagaac
     2461 aacttcatcg cctactttta catgggtttc agcgcccagg ttaatcatcg actgatccat
     2521 gcaaacccgg ccaacgacgg gataagaaac accgtcaatg agcactcgac ctgtgttcga
     2581 gaataaacgg ttgaacccat cagcgtagcc ggccgggatg gtggcaatcc aggtatcttg
     2641 tggcgcaaac caggtgcgtc cgtaaccgat ggaagtgcct ttctccacct tcttgatgaa
     2701 tgaaacgcgg gtgcgcaggc tgagaccggg tttgaggtca acgcgatgag aagatggttc
     2761 accggggtag aacccgtaaa tcatgatgcc ggggcgcacc atgtccatcc agctttgtgg
     2821 ataatcgagc acaccgcctg agttggcgca gtgcaccaga ggaacttttg cgccgatggc
     2881 gtcttctatg gtcaggacga ctgatttgaa ccggtcgatc tgggcttgcg tgtagtcatt
     2941 acccatcgaa tcgctcaccg gcagatgggt aaagacgcct tcaagcaaga ggttggagca
     3001 gcgtgagtgg atgaattttg ccagttcggg cgcctcacct ggtgtgacgc cgatgcggcc
     3061 catgccgcta tcgattttga ggtgcaccct ggcagtcagt tttagtcgct gacaaacgcg
     3121 ctgcagcgcc tgaatattcg ctttatcgca aaccgtcagc gtaagctcat tttcaacagc
     3181 acattccatt tcttcaggga acgccgggct aaacttgaga atcgggaggt ggataccggc
     3241 ctcgcgtaat tccatgcctt ccggaacggt tgccacaccc agccagttca cgccgatgtt
     3301 ttgagccatg cgcgatacct ccaccgcgcc gtgaccgtag gcgttggctt tgacggcaat
     3361 cagaatcttg cattgctgcc cgatcgcatg gcgaattcct tctatgttgt ggcggatatt
     3421 cgccaggtgg atttgcgcgt gagtttggta aagcatgcac ttctctatca ggaggatatc
     3481 ccgttaatca tgatttttcg aacataaggc gaggtactgg cggccagcaa ggcaccatac
     3541 cccgggtaaa aggcaacctc atcacccaca gcgagcggtt gttgtgcgtc ctgcacatct
     3601 aatagaagat ggtcgctgct tccgccaaga acgatgatgc cgggatcctc tggagtcagg
     3661 ttgtcaatca ctacatcctg ccggccaatg ttgcagatgg cgcgcagacg caccccgcgg
     3721 tcgacgaatt caggggttcc tccaaaagca tcctgtcctc gttcaccgat ggggacggaa
     3781 ggttttcttt ccagttcgat gatctcggcg accaaacgag cggtatcctg ccgtgttcca
     3841 ggaaaaggag aacggtcgag cacgttgcgc ccaagcagga tggtttcgcc aatacgcagc
     3901 atattgatct gaagtggcat tttatcagcc aataacaaag gcaggttagc gctgttgccg
     3961 gcgcataaca ccggcaaccg tatacccagg ctggtttcag actggtcgcg cagatcaata
     4021 agctgctgca tcttttccgt ggtgggaatg acaccgccat agcaggccag gttgcagccc
     4081 aaaccgttga cctcgatgcc tggaaggcga atgatagccc ttaaaacttc atccagcctt
     4141 gaaggccaaa tcccttcgcg caggtcgccc aggtcgacca tcaggatgac tttatgcgta
     4201 ctgtgagctg ccattgccgc cgcggatatt ttttttatgg tttccaggtc ggagacaagc
     4261 gagatgtctg ctgcgtggat aacctcttcc acctggctgg gggttgggat gcgcagcaac
     4321 atgagtggaa gagtggtacc gttttgccgt aacgacctaa ggttttccag gcgagaatcg
     4381 gccagcaggt cagcgccgct ctcttcgagc gcgcgcataa tcactggatg agcgcaaacg
     4441 actttgccca cacaggcaac ctggatacca tggtcatggc ataaatggat gatcgcgcgg
     4501 gcgttttcag cgatgcgttc tgggaaaagt tccaaacgag gtgtgaacaa cttactcaaa
     4561 tctccttcct gacccaatca acaaaagtgg atttcacttc atcggggtat ttttgcgcga
     4621 tggtacccag cgctgaaaaa cggtaggcgt catccagcag gatgggtgtg ccgggctggg
     4681 gaagcaggta tttgccggcg ccggtgcaga cgcgttgtag aatctcaact ccccctgtga
     4741 ccctggtaag cgctcctccg gtgccaatca cccttttcac cgcggtcagg tcttttcctt
     4801 tgaccacctg tttgcgacca gatggcgtat aaaggtcagt tacctgggca gcgtgacgcc
     4861 tggcgctgat ttctacggct ttggtgcata accagtgcgt cagttccagc tgtttatcgg
     4921 ttacggggat ggcctgcaaa tcagccagcc tatcttcact ttcccaatcc tggacaattt
     4981 caatgatgtg tttcgcgttg acataaacac ccaggtctcc ttcaaccgtc ctttttgcgc
     5041 ggggctctgg ttccagcaat ttcaacgacc attcctgact gccgtcagtg acgctatgca
     5101 catcggtggt tgccccaccg acatcgaata ccacgcaatc accaacctct tctgcgaaaa
     5161 gctcgctggc aagcagcacc gcgccgggag taggcaggat gccatggcgg gtcaattcac
     5221 caaggcgggc catcccggga gcgtggataa tatgtcggtt aaacacttca tgaatgatac
     5281 gcctcaaagg ttcgatattc aagacgtcca cttctggaaa cacattatct gcgatgagca
     5341 agtcgatatc ttgcgttttg aatatttcag taattggctg gcgcaaaacg cggttaccgg
     5401 cgtaaaccac cggcacctga agcccaaggt tggcgatacg acgggcgttt tcaagcacag
     5461 tctctttttc cccataatcc acccctcccg cgagaaggat aatgttgggg tggatttggc
     5521 ggatctctga cagttcgaat tcatccagca ggccggcggt tacttttttt acgattgcac
     5581 ctgcccccag ggcggcttca cgcgcagcgc gagcggtcat gttgtaggtt aagccgtgca
     5641 cggtcatacg caatccacca gcggctgagc tattgataaa aatttcaacc tcagggtcga
     5701 gtgggtttgc gccgtctttc acctgctgca tggccagctc aactccgggc attacgtctc
     5761 cctgggtgat actggtgggc gcgaacccct gcgccgcctg gacgagagcc ccatgttgag
     5821 aacgataaaa accgttcacc ttggtgatcg tcgaacccac ttcgatggtg agaatacaga
     5881 taggttcagg caatgggcac cttaattgag gggattaagc cagcccacgt ttctgcaggt
     5941 tcttgaccat ggcattgacc acgtggatac ccttggtgcc tctgccgaag gtggcatcca
     6001 ggccggtttc agccgccatt tcggcgttga cctgtgtgcc gccagcgatg agaatgagac
     6061 ggtctcgaac ccctcgctcc acacacaaat ccgccagctt cttcatttga gagcggtgaa
     6121 catcattgtg gctgatgatg gttgaaatca agatcgcctg agcgcctgtc tcgatcgcag
     6181 cgtcgaccag tttactcacc ggcacagaag taccgaggta atggtatttg acgccatatt
     6241 tttcgatacc gccgtgtttg atatcaagga tttctcgtaa acctaccgag tgttcgtctt
     6301 ctccgaccgt acctgccaca acggtcaacc cgactttttt cactacctgg cgcaaggttt
     6361 cttcttcgag catgatctct ttggcaggga ttttaaggtc agtgagatta agctcaaaat
     6421 tcactttccc tttgacctca acgaagcaac cttcagccgg atgcatgacc tgaatgttga
     6481 tgacctcagg gtcgatcagc cccagttttt tcgccatttc aacgccatat accctggcgt
     6541 agtcttcagg caaggggaca aagaactgag ttaggatggt gccgtctcca gcccattcgg
     6601 cttcgggttt gatctttccc cctttacggt agggataggt ctcctgtaaa cgcttttcac
     6661 aattgtcctc ggggtcgagc tcatcgatgt agacgatttt ttcaggtttg cacaacgtgc
     6721 agccctgaat ggcatcacag gggcgttgca actcagccgg gacgttgttg taaccaaaat
     6781 ggtcgcatac tggggcaaag taatccgcgt cacgctccac gatggtatct gcggccacgc
     6841 cgccttttcc atctctggcg ataccgtcac cgttgcgttc cgggtacttg gcagaatcaa
     6901 cgaagaaccc tttttcaacc gcagcaaaat agccgcctgt ctcaatgact tcctccagaa
     6961 aagccactgc gcgcactttc aggtcacgca ccatttcctt catcggacca ttggttttta
     7021 acgcgaccag atccttgatg ccgtctaacg cggcccaggt ctgtttgacc gtttggacgc
     7081 cgcggatgtt attgtaatgc cagggcacat tgcgaccctc atccggtgtg atggtactct
     7141 ggatatccgc gctggtaagc atggagatca gtgtgtcaat ggtatgcgta cggatggctt
     7201 cttcaatatc cgcctcgata tagcgggtgt tctgctgtgc gcgcatcttg aaatctgaaa
     7261 agaaatcacg caacgccacg gcgtaaggca ggtcatacca taacttggga gctggcggcg
     7321 cgttgggcgg gacagtggaa agaccgataa ggtccttatc catgcctacc gctactgaga
     7381 aggaacaatt gatgccgtgc tgcaccatta attcgggcat taccaaccaa ccggctttgg
     7441 cagtggcgtt ggcattgtgc gcgccatcaa tttggaagat acgggcgccg ttcatcacct
     7501 ttttggcttc tgcagcgtcc acaaaagaac ggtacatatt gacgttgcga tagagcacgt
     7561 tgtattgagg atcttgatgg gcgccattaa tgccttcttc ggcaaaaagc accgccactt
     7621 caggaccagc cacgcccgag acgtagctgt ggaagttgat agggcgtccg acttcttctt
     7681 cgatttggtc gcaagccttg cggctggcgc gaatctgttt acgggtgatg ggaaccccgc
     7741 ccactccttc aggcgtgcct tccatcaatc catcaatgtg actttgacca gtggtgcgga
     7801 tgaccatcag gtggtctgcc ccgtgccagg cagccatgcg catacggcgg atatcatctt
     7861 cgaaccgccc ggaggcgatc tcagtggtca ccacacaact tggctgcggg tcgatatggt
     7921 cgaagtactt ggaagcaggc aagccaatgc tgttcttcag cggtttagac atatcgtggt
     7981 aatggaaagg acccatctct acaccctcct ggggtatttc acgccaggtc cagcctttac
     8041 ggcgagggtg gtaatgctcc agtccttcaa ggatatgttc aatatccatt ttttgattgg
     8101 ggtcgagttt ttcgggtgtg ttggtcattt ttgtcccttt caaaacaatc ccgagaggat
     8161 ttctttcttt tcattgatta aagccgcggc ggtggggatg ctcacaccct gcttttcggc
     8221 aaccttcagc acgacatgac cagcgccttt tcccaacaga ccggcttcaa gcacacggtc
     8281 cactacgcca tgggcagtga tgctatcgat tcccatgcgt aacaacactg agcgctcaat
     8341 cgaaggggat gtatgcgttc gggcaagttc cacaattggt tccaccaacc tgtcgcataa
     8401 ttgccagaat tggtttttta actcctcatc gctgagttta tttaattctt tctgacgctc
     8461 tgtaaatcgt ttgatacggt cttcagacat tggtcttctc caggttttcg ttattttgcg
     8521 gaagggatag gaagttctcc ctgatccaat cgggggtttt ctttatatcc agtgccaaaa
     8581 aagagatatc actttctgac cagagagacg gggcaagttg tttgcccgcg ttttgcaggt
     8641 attcaaggcg caggcggtca atatcctggc ttttgccctg tacctgctca agtttttcgg
     8701 ggatgatgat ggttttacca ggaatgtcat ccagcgggtc accgcggcgg atctcaatcc
     8761 cgttttcgcg ggcaaaggct aactggctgt tctggtgttt ccctgcgcct gtgtattcgg
     8821 tctcctgaac caccacaacc tgttcgcgct ccatttggcg agccagcgcg accgcggcgg
     8881 tgaggctgat attcccggcg ggaccgcgtt caagaccttc gatcttggtg agcagttcgg
     8941 tgatgtaaaa cacctcaccc tgcgttacca gctggtactc gtccatgtag cgcaggctcc
     9001 tggcggcgtt acggggtaca tctacgcggt caggccaggt ggcaaatggc accccgaaac
     9061 cagtgtgacc tgtcgtgaaa gattttcgat tgaaatcatg gtcggatgcc atgtgcagcc
     9121 cttgaagatt gactgagcag gcaacaatgc gtgttgcgca gctgccagcg tgcagtaaac
     9181 cgcgagcggt tccggtaagg ttaccgccgc cagcgtgcgt gatgatgacc gcgtcagggt
     9241 cacggtggta ccttgcgcgg gtttgttctg cgatttccac ccccaggctt tcaaccccgc
     9301 ggataccaaa tggggtatac aggctggcgt tgaaaaaacc ggtttcttca agggtgcgta
     9361 aaagcatata aaaaagctca gggccgaccg tcaacttgag cacttccgcg ccgtaggctt
     9421 cacaggcgcg gcctttttca acaatttccg gctgaccgat accgcggcta tcgtacactt
     9481 cctgaacgat gatgcatttc agcttgcgct gagcagcctg cgaggcaacc gcggcgccgt
     9541 aattgccact ggtagcggcg accatgcccg cataaccttt atgtgctgct tcatacgcgc
     9601 tgattgacgc gcggcgggct ttgaaacttc ctgatgcatt tgccgcttcg tccttgacca
     9661 ggatggtggc gcctttgcct ggaggagaaa tctggcgtac cgcttccgtc aggttccgca
     9721 attcgtaaag cggggtattt cccactttgg tttcttgttg gatacgaaaa atctcatcca
     9781 tcgtgtagcc agtatcgcgc atcatggctt cgtaatcaaa ggcaaccggg ctggaaatgt
     9841 atttctcgta atccatgccc atggaagact tcatgatgtc attttttcgt gccatcacgg
     9901 cagcgtagga cacgtctctt tggctcattt ttcatactcc gttccgatgg taagcagttc
     9961 gggtacgact gtgccaaagc tatgatggta gctggggttc accactttta gctccccgct
    10021 caattcacga ccgataatgg ttttcacgcg cactttttgc ccgattttcg cgtcttcaag
    10081 cagaaaccct gatacccaga ggagataggg caccttcttt gtatcctccg ggagcgatgc
    10141 cgctcgttct tcaggtttta ataccacctg ctcaatctcg acccaggttc ctttaggaat
    10201 gatgttcatt gtgattgctc caagtagttc agcgctcggc tcaaacctgg cgccgatacg
    10261 ctgtttctcc catcaacgca gcgggggctg gcaggtcgat catgcgtttg aggcctgctg
    10321 tggcagcgac cacatgtggg atcatattga ccgcaagacc catggtggcg atacctccag
    10381 caatttcggg cttgatcgcc atattgatct ccggtttacc aaaaatatgg atgtaatctc
    10441 cggtatcctg tccttcaagt tgagggtgaa tctgttgagg gtggataaga cggataacct
    10501 ctttatctcc ccggtaacca atgccggtat gggcgcaccc ggctaccatt cccggttgaa
    10561 ccttaacatg aggggtttct cggtacacgt tgctgatgat gggctcccgc gcctgttcta
    10621 tacggtcaac gcccagaccc agcgcatcac tgatcatatg gatggactca ggaaagccta
    10681 catgcccgac gatggaacca tcttcaatgc ctgctttaaa ggcagcggga gtgatgccca
    10741 caccctggga tttcatcacc gttggaccgt atgggcttaa gtcattcacc cgcgaagctt
    10801 cgatgcgctc gacagtgtga ctcccacctg atagcatcac gaccagcagg tcaaggacga
    10861 acccgggatt gacgccggtc cccaataagg tgacctggtt ggctttacac aacacgtcta
    10921 attgcgccgc caattcgggg ttctgcgcgt aagggtcaga catttcttca gcgatggaga
    10981 tacaattaat cccggcttga atgatctttt taaggtcatg catctgttca gacagccagg
    11041 atgttgtggc gatgaccacg atatcaaccc gctctttgtt taggacggaa tcaggattgg
    11101 aagtgacggt tacacccagg ctgcgatcga tcttcaacac gtctccaagg tccttccctg
    11161 cataatcggt gcgggaatca actgcagcga cgatttgcag ccctgatttt tccagtatca
    11221 ggcgcgtcat gccgctaccc attgcaccca gaccccattg aagaacacga ataggcttca
    11281 ttttgactcc tgtgagtttt tttaattatt ttttgacaag caaattttag catagaagac
    11341 gcgaaggggg tgcgcaaggg atggtaggca ccttgagagc aggtggaatg tgctcaaaat
    11401 gcgaacacat tgaaatctac gggcaagcct ccgttggtga aatggtaaga tgcttgctgt
    11461 ggaggtaaac caaggttggc tgtaagcttt ttatcgtaac aaaggatgcc tgcctgccat
    11521 tgtgaaaaag ccgtagtaag gagattgcca aaacgggcat acaaattgcg taagtctttg
    11581 ctgccgctga tgcgtacgcc ataaggcgga ttggtgatca gccagccagg gtttgagcga
    11641 gggggagata attctgaaat ggattgacgc tggaaatgaa tgacctcgtt taaaccagcg
    11701 cgcgaagcat ttgaaatcgc agattgaacc gccccctggt cgcggtcata gccaaaaata
    11761 tgtgaatcct gccggataat cccatccctg gcagagtgaa ggactctctc ccactcatgc
    11821 ggaacaaaac ctggccagtg catgaaggca aaatcgcggg cgaccccggg cgggatattc
    11881 ctggccagca gggcagcttc gatagggatg gtgcctgaac cgcagaaagg gtcgatcaag
    11941 gtcatttctc cgctccaacc tgacagcaac agcaaagccg cagccagcgt ttcgcgtaag
    12001 ggcgctttgg tgctctccag tcggtaaccg cgtttgtgca acagagcacc tgaactgtca
    12061 atgctgatgg tgcacagatc attcaccaac ctgacaagca cgagctgccc attcttgctg
    12121 ggttgagtct cttttttaga gttggaaaag cggtcattga tggcgcccac gaccctttca
    12181 gctaccgcat cagaatgata aaggcgtgat ttgtggcagg taaccgaaag atgaacaggc
    12241 tgcccagggc tcagataacg ctcccactcc aggcgtgagg ctttcttccg caattcagaa
    12301 aatgcagcgg cgtagaattc cccgagcctg acgcttacac ggctggcggt gcgtaaatac
    12361 aggttggagc ggtatacatc ctccaaagag cctgaaagga gccatccccc tggatcatct
    12421 ggtaaaacac gattttttga cgctgccgtc gcaggctcag gttccaagat ggtcaggttg
    12481 agcgccttga gttcttgggc gaggatcatc tccagtccgg gcgcacaaga cacaaagaaa
    12541 cggagcgact caggcgacct aatcatggct tccgattacg ggagaggggg tgtttttcaa
    12601 atggcttttg tgtgccgccc gtctttgatc ggccggggtt ggtgatgatt tgcttctgca
    12661 gcgctttgcg gtgcaggttt ttttcaacaa agagcttttt accactgaag gggtcttttt
    12721 ctgtgtaata catcacgctg gcgtaagtcg atggggtagg ggtaaaaatt tgaacctgtt
    12781 ccggttggac tcttaattcc ttggtgacga attcacggag ctttttcatt tcccgctcgc
    12841 tgcttccagg ataggctgca ataagatagt agctgagaaa ctgtttttta ccggcctggc
    12901 gggaaaattc gaggaatttt tcacggaaca gccgcagcga ttcatgagac ggtttgccca
    12961 tcaggttcaa cacaactgga tcagtgtgct cgggggcaac ctttagttgc cctgaagtgt
    13021 gatagcaggt gatatctttt agatattgca ttccagaccg gcggtcagcc agtaccaagt
    13081 cataacgaat gccggaacca acgctcactt tttttactcc tggcaggttc cgtaatttac
    13141 gtaaaagggc aacctgcctg gaatgatcaa ctggcaacgc agggcagata ttgggaaaaa
    13201 cacagcgttt atcctcgcag gcaccggaga ccagcttttt gcggcattca aacccgtaca
    13261 tattcgcagt cggcccgctc aagtcagtga tcattccttt gaaatcaggg tgatgagaga
    13321 gcttctcagc ttccttcaga atcgaagctt cgctgcgtga taccaccgtc ctcccttcgt
    13381 gaacggctat ggcgcaaaaa ttacattcgc cgtagcagcc ccgatgtgtg ggaatggaga
    13441 actggatggt ttgtaaggct tttacctcgc cctgcaattc gtaataagga tgatgtaacc
    13501 gttcaaagcc caggtcgtaa atggtgtcga gcgcttgggt tgatggggtt ggaggagggg
    13561 gattttgcac taggtagcgg tcgcctttct tttgcaccag accttgcgcg gtgagggggt
    13621 cattgttgcg ataaaaaagg tcgaacatat gaatgaacgc gtcagggtca gcggatactt
    13681 cctcaaaaga tggcagttcc agataggttg agggggcttc agcagccagg tagcataaac
    13741 cgcgtatttc acgcggcgaa cgcttttgtc gtaaggcgag ggccagctcc acaatgctgc
    13801 catctgccat cccgtacagc agataatccg cttttgagtc gaacaggatc ggcgcgcgca
    13861 ggcggtcaga ccagtaatcg taatgtgtga tgcgcctcag actggcttcg atccctccca
    13921 ggacaatggg tacggtattt ttgtgataac gcttgatgag gttggtgtaa gcgatgactg
    13981 cacgatcggg ccggcgaata ttggctccac caggggtaaa atcatccgta cggcgtttgc
    14041 gcaacgtggc tgtgtaattt gcgaccatgg aatccactgt acccgcgctg accccccaaa
    14101 aaaggagcgg ttcacccagc ctgcggatgt cttccgagcc ggacatatcc ggttgggcaa
    14161 tgatcgccac cctataacct tgtgaaatta aaattttccc gattaccgcc aggccgctgt
    14221 acggggtgtc gatataggaa tcgccggaaa ccaggatcac atccggttgt tcccagccca
    14281 aatggacgag ttcttcacgc gtggttggaa tgaacacgat agttccctgt tgtcgaacaa
    14341 actgagtata acatctcaaa cgcgaagtaa aaaaaagcag ccgataggct gccttgatca
    14401 ttcatcttca agttgttcgt gttcgtccag gtggacatga tcgtggttca gctcttcgct
    14461 ggttggcagg cggagggcga taatcttcac ctcgaattcc aaatcgcggc cggcaagcgg
    14521 atggttgaaa tccaatgtta cagaatcggc agtaatttct tcaataaacg ctgaggattc
    14581 aattccttca tcatcctcga cggtgacttc catatctact tcgagtggaa aatcctctgg
    14641 gaattcactg cgggggattt caataatatc ctcttccgaa tagacgccgt agccatcctc
    14701 tggctttaca ctcacatgtt tcgattcacc aatgttcatt ccttcgatgg cagattctag
    14761 cccggtaata atatgcccgt gcccctggat aaactgcagc gggtcgtctt ctgtggcttg
    14821 atcgatgagc tcaccttcga ttttcaactc ataagccata ctgacaacca ggtctttgga
    14881 tactttgcgt agatcgtttg atgggttcaa gttagctcac tttctttttc gtagaatgaa
    14941 tgagccatga ttatatcacc cgggggtata aaaaaaacac cgattggaat gtgaaaacag
    15001 gtgaggaggt ttactgcccg ataaaaactt cgcagctgcc tattttctcg aaactggcca
    15061 atttgttcac tgcctgcagc cacaattcgg tagacacgtt cgtagaaaag tgctctggca
    15121 gcaggtcggc ccaatgaatt tgattgatta cctggtcaat tccattttgg ctgatttgag
    15181 cgcaaaattg agagattacc ttctgaccat gcgagagaat tgtctggcgc tctaatgggg
    15241 tgagaggtgg ttgagaaggg gaagaggggt tttccagccc cagccaggtt tcaattgaag
    15301 aagccgaaaa attgtcaacc agaaaatagc cgtttgtttt tatgtggaac ttgcgctcga
    15361 tcttcctgat cacccggtca ggtattttgg cgtcacgggt caaacggaaa aaggagaatg
    15421 agtcgtcttc ttgctctgta tctgcgggtg ggtagagcga gacaaacgcc aggtgagggg
    15481 gggggcatca cgcacgaaca acccccatac agataccagc ataggtgaat ccccacccag
    15541 ttctgaaact tgaaagatta aataattttg ctgggctgat ataggctcaa ggctgttgtt
    15601 gggggctgtc tttttcaggt gaccatccaa gtgagcagct gcctggaatc caaaatagac
    15661 agacaccaaa aagagaaggg cgatcaatgc cagggcgatt ttcttcatgt gaagacaggc
    15721 tttcaaataa gtgacagatg ataagattct atcatctgtt tgacagatac atccctaatc
    15781 aaccaaggcg atggattaac ggtcacatga ccagcagggt aacagagtaa atcagcagcc
    15841 cgctggcgaa acccacgcaa aaaacgagaa gcacgatggc aatttttcgc atcatccctt
    15901 tatcctgtga agccagatag gttgatcgca caatggtgcg cagcaggctt gatttggtca
    15961 acaacgtgat gatgaggttc cagaaactca gttcaatcga gttgataatc tcagcaatca
    16021 tcgcaccctc tatcttgaag tggtgagctt caggatcttg ccattttcat ctgaaacaac
    16081 ccgaaccgta cgtgaaagtg ttttaccatc ggctgttttt gcctgcccat ggaagatcaa
    16141 ctgatattta tcagcaggta aggctttcac ccgtggagga acccctttta attcggggaa
    16201 ctgcctgtaa acggaagtgc aaacggcgtt ttttatcctg gcgtccatgg cgctcctttc
    16261 acctggcttt gcgccgcata aaggccggaa cgtcgagctc cgattgggcg gctgccagct
    16321 cggggtgaac atccggaata ttctcctggt aagaattacc gggagagaga gaggtttctt
    16381 tgtctgtgtc ttcagcaggt gtcatcacag gtacagggcg cttcatccgt tgtgggtcga
    16441 gcgcagtggt gccaatgccg gttaatacca ggatgacctg aacccggtct tccatcctgt
    16501 catcacagat gatgccaggg acaatctcgg ccattccacc gctcttttcc tgcaaggtgt
    16561 tgagcgcttc cgtgacctca aagaagggaa ggtcgttacc accagtgaag ttggcaatga
    16621 tcccgctggc gtcttccaaa tcaatagatt caagcatggg gtggtgcaag gcgtgttcga
    16681 gcgcgtcact tacgcggttt tcacctttcc ctgttccaat tgccagcagc gagccaccac
    16741 cattcagcat catgttcttg atatgcgaaa agtccacatt gatcaaacct ggctgggtga
    16801 ttagttcgga aattccctgg atgccctgcc gcaggatatc atctgcgaga cggaacgcca
    16861 tttcaatagg caggttacgt ggagccacct gcaacaggcg gtcattgggg actgtgatga
    16921 gcgtatctgt gtgctgctgc agcattaata acccattgcg agcgttcgtt tgcctgcgcc
    16981 caatttcaaa ggaaaaaggc aggctgacaa ccgcaaccac taccgccccc acggacttgg
    17041 ccacccgggc agcgacggga atggcgccgg tacccgttcc gccacccata ccagctgtta
    17101 ggaagaccat atcagcgccc gtaagcgcgc tagtgagttc agcgatgctc tcttctgcgg
    17161 cagcctgacc caccgccggg tcgccgcctg caccgagacc gcgggtgatt tttcgaccca
    17221 actggatctt gaggtcagcc tggctgtgtt gcagcacctg agcatcagaa ttagcggcga
    17281 taaattgcac tccttcaagc ccaagttcga tcatacggtc gaccgcgttt tgaccgccac
    17341 ctcccagtcc gattactttt agaactggcg gattggcttt gatgggagtg cgcgcaacgt
    17401 gggagtggaa ggttgagtaa tccatgttta cctctctgcg agttgagttg caatacttgt
    17461 accatcccca ctgatacgct caacgacaga accggaacgg cgtaattcct caagagaggt
    17521 ttcagggaag gactgttcac cgcctattac ggtaacgcgt ctggcaagag ctgcttctcg
    17581 gattgaaaag ccaatcgtag ggcggtgttt ctgaacgaag gcgcgggtaa catcgagatg
    17641 aaagtcagcg acgccccatt catacacagg gataagcagg taatgatcga tcgggtggcc
    17701 tgtgaaggat ttaattggcg tggattctgc ttcaacaagg aggtctgctt tttctaaggg
    17761 tacgttcgca tggttggaag ctccaggcac ttgagcctgc atcaacaggt cagccacggg
    17821 caaatagtct tcaggagatt tataccaggc gcaggcaaac tcctccgagt ctggttctgc
    17881 ggagagtaaa taaaaactac aacttatcac ataatcagga atggcggttt tcgaagaatt
    17941 ctctttaagc atttccatga tgcttaagca gattccggca tgcacctcgg gagagtaggg
    18001 ttgacctggt tctcgaattc ctgcgcccaa catgaaaatc ggtaaatcct ggtcacaaac
    18061 tgttcgagcc gtggcctgca gccattcaaa attaaagaat ccacgttgat cctgtccttg
    18121 cgcatcagtg atatagggta atgattttgg ccatttctgc ggcccgcccg cgccgtaatc
    18181 caactcgtga ttaaaggtga aagcgtaaga agccaaaccc atttggttga tcaaagatga
    18241 atgaccgcgt ctttgtattg actgaagaga ttgcttgagg aaggaaaggt cccagtaact
    18301 tcctccgggt tggaggggtg ggaaaatggg catcatttcg gctttgactg ttgccgtggc
    18361 taaaggtaaa aagcggtcga cgaacctttc aacaaggttt tgttgtaccc atccggcagc
    18421 ggaccagttc gccatatcat tcgggcgatc aaaaagaatt atgtgtttca caccccaacg
    18481 cgcgtaggcc gccagaatgg ctttcaggtc tgcgggtgaa ggggtgttgg gcagcggcaa
    18541 gggaatgtga accaatgggg taataccggc tttgattaat gcggtgatga actgttcggg
    18601 gatggcgcgg gaggtatcag agaggagcac caaccaggcg cacttgagcc gctgcagcac
    18661 aggcagccag ttggaaaggt ctttattcgt ataatgggaa gtatccggga agtagtggat
    18721 tccgacccgg ccagtgacga gttccctggt tttcttgctt ttcaatatca tgcggcacct
    18781 cgccaaccct acgttttacg taaccggaaa gttgcaagaa atgagccaga gcaattggca
    18841 caaccctaat tctgctataa ttccctcgtt atgatccttg tcacgggcgg tactggattt
    18901 atcgggcggt cacttattca ggcgctggta tcttcaggcg aacaagtacg agttttattg
    18961 cgcccttcga aaacatctcc caacttccct aaaggcatct ccgttgaggt agcggtgagc
    19021 agcttttcag accagcgcag tttgcgcgct gcgttgaaag gggtcgattt tttgtatcat
    19081 ttcgcagggg ctgaacgtca aggactcaac gcgaatttga atcagatcga tgtggaaggc
    19141 accgccacat tgatgcgcgc tgccaaagac accaacctca aacgggtctt ttatttgagc
    19201 catcacggcg ctgatcgggg gtcggcttac ccggtgatga aggccaaggg catcgctgag
    19261 aattggatta tggatagcgg tatcccttac acgattttga gaacaggagc agtatttggn
    19321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tccaaaggaa accgctcggc
    19441 gtgacgcctg aatttctgcg cgggttaacc ttgtggatcg cccaggttta cccgaaatta
    19501 ccagtatcca tcttctggct ggattatctg gcggttgacc gcattaccag cctggattca
    19561 gttccacgtg agttcggttt gattccagcc aggtttaaaa acaagatcga ttatctgaat
    19621 aagaagattt aacaccgcat gcctgaaaat cgagcgttct gcatccatgc ccatttttac
    19681 caacccccgc gcgaggatcc tttcacgggg aagatccctg aagaagcggg tgcggcgcca
    19741 tttgccaatt ggaatgaacg cattctggcg acctgctacc agccaaatgt ggaggagcgt
    19801 aacttcagtc agttgagctt taatcttggc ccaacactcg tcgaatggat gagccaacac
    19861 gcgccagatg tactcgatag gattgtccag gaagacgcgc ttaatgccac cctttatggc
    19921 actgggaacg cgatcgctca accataccat cacaccatcc tcccactggc gagcaatgaa
    19981 gataaacgca cccaggtgtt atgggggatt gcggctttta atcatgtttt tggcagggct
    20041 cccctgggaa tgtggttacc tgaaacagcc gtagattatg agtcgttgcg tgtgttggca
    20101 tctgctggta ttcaatttac catgctggct ccctggcagg ttgaggtgat tgaaggagaa
    20161 tcaccctatt tagtcgaact gggtgcggga aaatcaatca tcgtgtttgt gtatcatggt
    20221 gggctgagct ccactgtcag ttttgatgcc tttgcgacga gcaatgcgga tgcttttgct
    20281 gagttctact tgaaaccgga aatcgaccga ttcaacccca atcaattttt attgatagcc
    20341 actgatggtg aattgtatgg tcaccatcaa ccctatcgcg aaaaatttct ctccaggctg
    20401 ttggatggat catccaacgg cattggattg cgggcgacat atcccgcttt gtggatgaaa
    20461 aatcaccagg taacgggaag ggcgcgcatt gtagaaaaca cgtcttggag ttgttttcac
    20521 ggtattgacc gctggcggac tgagtgcgat tgcacggctg ggagtacctg gaaagcgggt
    20581 ttacgtgaag ggctctccct gattgcggca cgcatcgacg acgcctttag cgaaatttgt
    20641 gcagcgcatg ggttggaccc cattaccgcg agaaatgact atatccaagt aatgcttgga
    20701 atggacactt tcgaacagtg gctgtcaaaa acaagcggca aagaggtctc atccattggt
    20761 aaacgcaagc tggccacgtt gtttagtgcc caaaggttgc gtcagcaaat gtttagctcg
    20821 tgcggctggt tttttgacga attagcacgt attgaaccgc gcaacaacct ggcctacgcc
    20881 gcgcacgcgg tcgttttgat ggagtcactg accagcatgg actttcacag cgtggcgcag
    20941 cccgcattac gcgaagcggt cagtcataca agcggtgaaa ccgcctttga cttttttatt
    21001 tttgcctacc aacggttcaa gaatttacag aagactgccc aggcgaatgt tggttgaaat
    21061 cgaatagtcg gtcgaactga tagcctgatt ttgcttctaa cttcttcaac ttgcccaaga
    21121 gctgattaag attcaagaca acctcggtga tggtggaagc atccgcgcct gggtttgcta
    21181 tatactggta aagtgcgatg agagacagcc accagacaag ggtttcaaat gattcacggt
    21241 tgtaccacag cacatcctgg taacggttca cgcgcaacag ggtttgcacc tgcgggtctg
    21301 taaaccagcg gacgattatt tcctccattg gttcattttt cgtcgcgttg taccatccct
    21361 ggtgggtgat gccgattcgt aaaagaaggg cgctgttttg gatctcctgg ctggtaacgc
    21421 ccattgattg cagtacctca atgaaagttt tattcaggcg gaattcgtca agccaagcga
    21481 gggactgttc tgaaaaatct tcctggctgg caactttgcc cagccggctt gtgagtgaca
    21541 gcatcaacaa agttacccgc agggcagggc tttgctccaa atggttttca agcgcgtcaa
    21601 gaaacgtcga ggttgaaggg gagagtctag tgacgcgttt tttcaactct tgagaaaaca
    21661 tgagagcagt caagaagaga gagctggttt cctgttggag actgcgtaat tcacctgagt
    21721 taccagtgta aagcgccacg ccctgcaaaa atctgcctat cctttcctgt atttcccggg
    21781 cttcagtact gtccaacgtc gggctggtgg aatatttttg cagtaaatac cccaggtaac
    21841 cttgattgac aatcacctgg aagggttcca atactggttt aagggtcagt tcgttcaacg
    21901 cctgggctat atccggcacg ccctgtctac caatcagtcc gtataaagat gcgtaatcat
    21961 gagcagaatc agcatgaaca acctgaaaat ctagcagggt ttggtaatga taagcgtgaa
    22021 gccggaagaa aaaaccacga tctaacactt catccaaggg gcgaaggtat tgaagttgtg
    22081 agatctggtc acgaaagacc agccagccgt tacggtcagc cggcaaatgc agcgcttggg
    22141 cgattgagac ctgttccagt tttttttcct gtccataaca gcgaagctga ggaacggaag
    22201 tatgcaccca accttccgtg tcttcatacc gattattaaa tacgatgagt gcggcatttc
    22261 cagcgtcata gttcgtatag gcgtacacat tctcgttcac gttgccattg ttcgtgaaaa
    22321 agtcatacag gttgaagtga tcaaccccgg cgaacaggcg gcgttggtgt aataagggaa
    22381 aaatttctcg ttcatgcctg gagaccaaat cgttatctac cggttcatcc cagtaggctt
    22441 tacggtattc cataccgtat ttctccgaga acccttcaat ttggccgtga cccagcatag
    22501 gcagaccggg catggttgcc atgagcatgc agatgccgaa atacttatca ctttttccga
    22561 attgctcgac ggcagtacgc tcatcgggat tgttcataaa attgacataa cgtttgagaa
    22621 tttccgggtc aaattccaaa gtgttgcgca ttagccggcg gtagccggca ttgtcttcat
    22681 tgcgcagcat attcatgaaa gcgctgttat acacccggtg catcccgagc gtacgcacaa
    22741 aatagccttc cataagccag aaggcttcgg cgagcagcag ggtatcgggg gcttctactg
    22801 ccacccggtc taccacttca cgccaaaatt caaccgggaa caaacggttg aactcttcag
    22861 cagtaaggct atgctcggaa cgtgatggga tcgcccctcc ggagccaggc accgggaacc
    22921 agaggcgctg gtaatgtttt ttcgccaacg tcatcgccgc gtcaaaccgg ataattggga
    22981 acctgtgggc aacttctaaa atagtttgaa tgacagcctc gcggacctca gggttgagat
    23041 agtttaactg ggcggtgtca ttccagggca tcgaggtgcc atcgtttcca tggtacacat
    23101 agtccacctg tccattacgc aaatcctggc gtttgaacac cacagcggcg tctgtacggt
    23161 catagtaatg atcttctatc tgtatcctca catcaggatg cgaggaaaga ttttccccat
    23221 tgaaactgta agaaggatag gggctgtaat ctaaagaaag gaagcggtca ggatgatgga
    23281 ttacccagtc ggaatcaatg cccatgtgat ttggaaccat gtcgctggcc agacggatgc
    23341 caaagcgggc agcattttct tttagcgcta aatacgcgtt ttctccaccc aggtcagccg
    23401 cgatctggta agaatgcagc gaatacgccg aagaaatggc ttctgggttg ccgcataatt
    23461 ttttaatgcg ggcagaagcc gggctgcgtt cccataaacc gatcagccat agacctgtta
    23521 ttccccaacg agataaagtc tcgagctcat caaggggaat ctggtcaagg cggtttatct
    23581 cgcgctgata ttttttgctc aactggtcga gccacacata gctattcttc gccatcagca
    23641 ccagccgtgg catccattca cggtctttgc tgaacgcctc aatctcagaa gaaccgcctg
    23701 cctgaaaaaa tccatcacgg tcataaaccg ggatggccgt gacgcctggc ccggtgatgc
    23761 caagcttgtt ttcttccttg ataaggtcaa ggctggaaag caaacggtac aggtaatctc
    23821 ccagtaacat agcccaatgt tcgcgaatgt actctaattg ccctgtgatg gaatcaggga
    23881 ctgcaatcgc cgggctgcgc aacatattcg tcaatggaaa gttggaaggc ccaaaatatg
    23941 gttgcagagc aaaaaagtga ttaatctcag atataaacag ggcgtaacgg gaatcgttga
    24001 taaaggtttt ttcggcaaat aattctctat atgagtcaag ggcggggttt tcgagagaaa
    24061 gccagagcat gattaattct tcaattaaca gttcccgatt agaaacgccg tcagtgttcc
    24121 caagcaaata ttcatgggcg ccaacctgtt tttgataaac tggcgaggga ggaaactccc
    24181 gtataaacaa ttcaaggaaa gcattcaaag cggctgagga tgattgctgt tcgagatagg
    24241 cgagcgcttg ttttaaaacc tgtggatttt tctgttcttt atatagtttg aagatgtggt
    24301 ggaatatctc atcaatcaaa cccatggcgt tcacctggct ggctttggca gcctgttcgg
    24361 ggaatgtgac caggtcccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
    24481 cgcataaaag tattttagag cgtccaatga gataagcgtg ttcttgcgcc tgagcaccct
    24541 ggcgtaatac cagactcttt ggtcgagaag agagtccttc cagtgccagg tgatatgccc
    24601 gggatcatcg tgctcgtcgg tgactggcct gtcacctgct accgctcccc aaaggctggg
    24661 gaaggggaag cctttcacag gccaaaaaaa gataaaaccc ctttctttca catattcaac
    24721 cgcctgttca ggggtagaga gggaaaagcc tggcgaaaac cgaaaagtca gcgcccggta
    24781 atcagtcaga cgggaagcag aaacaattgc cattctcaat ccagctccaa tcatatcagg
    24841 tttcatgaaa ggagctgtat tgagcgacgc aactaatctg ttactgcaaa aatgtgagaa
    24901 atgatatttg aaccgctgcc gccgatgttt tgtgtcatgc caaccctggc gttttccacc
    24961 tgggttccag cagcttcgcc gcgcagttgt tctacaactt caacaatctg gtacatacct
    25021 gtggcgccta ctggatgacc acgggctttt aacccgccgc gagtggcaat tgggatcctg
    25081 ccattggtga ggatctcgcc atcgagcgca agttgggggc ctttcccttt ttctgcaaaa
    25141 ccattggcct caagggaaag cgtcgccatg atcgaaaacg cgtcatggag ttcaaaaagg
    25201 tcgatatcct gcggtgtgag ggaagactgc gcgtatgctt tggtcgctgc gttgtatgcg
    25261 gctttgagga acagagcgtc tttgcggtga tggacagcca tcgtatccgt tgccgctgaa
    25321 gaagctgcta ccctgatgac tttgcgcccc ttgttttgat ggaatttttc taaaggcaca
    25381 accagggccg cggcagcgcc atcaccagtg ggagaagcat ccatcaggtt gatggggtcg
    25441 gtgatcatgg gcgcttttaa ataaccttcc actgtcactg gttcatgaaa acgggcgtaa
    25501 gggttatgca tggcgtttgc atgggcgttg accgcgaaag gcgcgaagtg ctctttctcc
    25561 cagccatact cgtacatata gcgtttcatc aaaagggcgt tcaaagacac aaatgaaagc
    25621 ccattacccg cttcccattc cgcgtccgca gctgtggcaa gggcggcggt aatctcattc
    25681 aggggagaat ccgtcatctt ttcaaccccg acagcgagag cagagtctac cagaccagag
    25741 gcgacagcca ttaccgcgga gcgaaaactg gcagctcctg aactgcaagc ggcttcggta
    25801 cgaatcgctt ccgccccgcg cataccaatc caatctgcca aatacgctcc caggtgttgc
    25861 tggtggttgg cagagccaga catcatgttg ccgacgtaaa cagcttccac ccgctccaga
    25921 ccggaatcct tcagcgccgc cagggcggca tcgcccgcca gttcacgaag cgatttatcc
    25981 caatgctcat caatttttgt ttggccaatt ccaataacag caacctgtct catcttcacc
    26041 tcgttgttcg ttctaatgag attataccca tgcaacctat caggaactat cagtttacct
    26101 ggttttacaa gaagaccggg actgccttat aatttgggaa gccatcgtcc aggagaaata
    26161 tctgtgcaca tcgctcaacg cttaaaaata tcgctagttc tcttcttttt cgttctctgt
    26221 gcttcttgcg cgggtattcc cacgcagagt caggaaatta cctctacgcc tacttttttt
    26281 caatccgccc cgttacagcc gacgcccatt ccagaagaac cggtattgtt tgtttctaat
    26341 ggagttccag aagactggct gaagaagata gacggctttc cgcacatctc gaaaacggaa
    26401 caaaaagagc aagccaacct tttcttcact gttcatattg gaaaatttat agaaccccag
    26461 gtgttcagca gcaccagggt ttacgcggtg gtggagccat ttttcaccat ccgtgatgaa
    26521 atcagttcga atgagctcaa agacctgtgg tcaggcaagg ctgtggaagg tcaacatcaa
    26581 actcttgtgg tcactgaaga gacagcacaa gtgattggtg ctgtgtgggg aaaaccggca
    26641 ggagaggtgc agatcatccc ttcagatggg ctgctggcgt attgcgaaga gcacccggag
    26701 gcgctggcta ttctcccgtt tgaggattta agcccccgct ggaaagtgct taggcttgat
    26761 gggttgagtc cgctcgacaa attcctggcg gttgaccggt atccattgac catccactat
    26821 acacttcaat cacacttgag taatccatca atggtcagcg agatcgttga attattttca
    26881 gacgcactgc cgctgacgaa tcgagacgaa tccaaaatga ccgtgctcat gatgaccgga
    26941 actaccgcca tcacccgggt tttggcttat aagatcagta ttaagggaat ggagtacccg
    27001 attgctgcgg ttcaagattg gttcgtgcac gctgacttga agcatgtgag caatgaatca
    27061 cccctggtgg aaggctgccc ggtaccggat gagttttctt ccagccttgt attttgcacc
    27121 ccgccggaca tgattgaagt acttgaactt ttgggtgtga acattatcga actcaccggg
    27181 aaccatatca acgaccacgg gccggaaaac ctggagtaca ctctcaaggc ttaccgtgac
    27241 cggggatggc atgtatacgc cgcggggaat acagttgaag aagcgcgcag ccctctgctc
    27301 atcgaaagta atggcaacaa agtggccttc atcggctgca atgctgtggg tcccgcgact
    27361 gttttcgcca aggagggacg ggcaggcgcg gctgaatgcg attttgaata tttattcagt
    27421 gagatccgcg agctgaaaca aaaggggtat atcgttgtga cgacttacca gcacgaagaa
    27481 gaagaaaact tgatgtattt tgaaaaaatc agcaaggatt ttcaactcgc agccgaagca
    27541 ggcgctgata tcgtacaggg ctcgcaggcg catagcccga tgggttttga atttgtagga
    27601 gatcacctaa tccattatgg tttaggaaat ttcctgtttg accagatggt agaatggaac
    27661 cggcgcgaat tcattgaccg ccatatccta tacaatggaa aatacatcaa cactgagtta
    27721 ttgaccgcca ctctggtgga ctggtcgcgt ccgacaccga tgacggaaga ggagagaacc
    27781 caactcctga aggaaatatt caccgtaagt ttgaagagga aaaatgaaaa taaatgatgt
    27841 aacgctcaca atacgagaag gcatggtcgt ttggccagga gaacccgaag tcgatttaca
    27901 ccggatcaag aaactcgaag aaggcgccaa cgccaacgtt actttaatgg gtttcgctgt
    27961 gcatactggc acccatgtag acgccccccg ccatttcatc gctgatggta attcagttga
    28021 cctgttggat ctgaacatgt tgatcggcga ggtaactgtg gttgaaatcc ctcaagacgt
    28081 tgatcatatt tcggctgctg taattgcccg atcaggttta gcggtcggta ctgagagagt
    28141 gttattcaaa actcgtaact ccaattactg ggttgaaaac aggggagatt ttcagcctgg
    28201 ctttgtggga atcactgaag atggagcgca ggcgctggtg gaattgggtg taaagctggt
    28261 tgggattgac tatctctcca tcgcgccata caaaatgagc cgtccaaccc acctggtctt
    28321 gttgaaagaa aaaatggtgg ttatcgaagg gttggacctt tcaaaggtaa aaccagggaa
    28381 gtacacgctt tattgcctgc cgctgaaact ggcgaatgct gacggtgcac cggcacgggt
    28441 gattctgatc gaagattgag ctgagcggct caatcctcag gttgacgata gcgcagcgtg
    28501 aatctatcag tcaaagatga accagattcg tgctgcccgg attctattcg cgcccgcaaa
    28561 tcattgaggt cagacaccag tcttggaata tccagtgtag tagctgcgcc cacaaagggc
    28621 gtgagattga gcagggcttt ttttaatagc ttcaatgcgc ctttcgtatt tcctcttgca
    28681 agatgatata acaacacgct gacctgaacc agcccctggt acaactgtcg ttgttttttt
    28741 tgctcgttcc tccaggcaag ctcaagaacc tcatgcgcct ggaaataatc tccctggttg
    28801 attaagttga tccctaaatc aacttctctt ggaaaagtca ggtcacctgg tggcatagga
    28861 ttactctacc acgaaataga ttccaggaaa cacgatttat gataggataa tagtggattg
    28921 tctcatctct tttggcaaca gagggaaaaa tgatagaaat ggctgatgca gatatactgc
    28981 cgtattcgct tgagcataac ttctggacct ggtccgcgca agcaaaagtc aagcccatcc
    29041 caataaaatc cgctaaaggg gtttattttt gggacgtgaa tggcaaaaaa taccttgatc
    29101 tgaactcaat ggtgatgtgc gccaacattg gccatggcga cgaacgggtc atccaggcga
    29161 tgattgacca ggcaagagaa ctgcctttcg cgggtccgca atttgccacc aaaccgcggg
    29221 cgatgttggg aaaaaaactg gctgagatat tgccagaggg gttgaccaaa tacctctaca
    29281 cattgggcgg agccgacgcc aatgaaaacg cggtgaaact ggcacgcgcc tacaccggaa
    29341 ggcataaaat actcacccgc tatcgctcat accatggcgc aacatttggg gcggctgctt
    29401 tgaccggcga cccccgacgg atggcctggg agccggctgt gatgccggga gtggtgcatt
    29461 ttcttgaccc gtttcgatat cgttccactt tccatcaacg ctacccagat gtcgatgaag
    29521 acgagttctc gcgtgattac ctggggcacc tggaagaaat catccaatac gagggaccgc
    29581 agaccatcgc tgccattttg atcgaaagcg tgacaggtac gaacggtatt attgttccgc
    29641 cagaaggtta catccagggg gttcgtgata tttgcaatcg ccatcacatc cttatgatcg
    29701 ctgacgaggt gatgagtgga tttggacgga cgggtgaatg gtttgctgtg aaccattggg
    29761 gcgttgtgcc ggatatcatg accatggcca aggggttgac ctcgggttat gccccgttgg
    29821 gcgtggtggc tatgaaacct gaaatcgcgg cggcgtttaa cgagaaagtg tatgaaggcg
    29881 gactaacgta taatggacac cctatctcac tggcggctgc cattgcagta atcgaggtga
    29941 tgcagcaaga tcgactggtt gaaaaagcca aattaagtgg cgagatattg aaaaatatgc
    30001 ttcaaaattt gaaagccagg cacccatctg tcggtgacgt aaggtcaata ggcttgtttg
    30061 cggcaattga agtggtaaaa aaccagcaaa ccaaagaacc ggctgcgccg tttggaggtt
    30121 ccagccctga aatgagcgcg ttacgccagt atttgctgga tcatggcgtt tacctttaca
    30181 cgcattggaa caccatcctg atcattcctc ctttgattat cacccagtcg gaacttgaac
    30241 aagggctgag cgttatcgat cagtctttat acgaaatgga taaggtggtc aatcactgat
    30301 ggaaaagatg aaagaagagc ggaaaattga atctgtggtt atcctggggg cagggccggc
    30361 tggtttaaca gcggcgctct atgcagcccg ggccgaactg acgccgctgg ttattaccgg
    30421 tatgcaattg ggagggcagg cttctttaac acaccaaata gagaattatc ccgggttccc
    30481 ggaaggaatt ggcggagcag atttgggtga cctttttaaa caacaagccg aacggtttgg
    30541 cgccaggttg gcgtttgatg tggtgactga ggttgattta agccagctcc ctttccgcat
    30601 taagacgtac ggtcaagaga ttttttcaaa aacggtgatt gtcgcgacag gcgctgaccc
    30661 cgtacatttg agcgtacctg gcgaaaaaga actgaccggg aagggcgttt cttattgcgc
    30721 gacctgtgat ggatatttct ttaaaaataa gcatgtagta gtggtaggag gaggggacag
    30781 cgcgctggaa gaggcgatct tcctcaccaa atttgccgat aaagtgagtt tgattcatcg
    30841 cagggacact tttcgcgcgg gtgcgatttt gcaatcacgc gtacgtgaaa atcacaaaat
    30901 cgaatgcatt atggattcgg tcgttaagga aattaaaggg attgacaaag tcaacgcggt
    30961 gatcattcag catgtgaaaa ccgctgcgct aacagagtta ccagccgatg gtgtttttat
    31021 atttattggt cacgcgccca ataatggcat ttatcaaggt caannnnnnn nnnnnnnnnn
    31081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31141 nnnnnnnnnn nnnnnnnnnn nnngccaacg gaagaccatt gcagggtgat ggactcattg
    31201 acgttggtaa aaaccgcgcc atcttcaggc agcaacaggc tggccgctgg gtaagggggc
    31261 agcagggtgg cggtgggtgt ggggccctga gtgggcatgc gctcgcaaag cggtaggctc
    31321 aggttcatac caacccaaac ggtgtcagag gtgagaccat taaaggtctt gatcgattca
    31381 atgctgatgt tgtaattagc cgaaatagag ctgagggtgt cattttccgt tacctggtaa
    31441 ggaacggtgc cgcaggcttg ctgagtggct tcagcagcgc tgagagtggc ggtcggtaac
    31501 gcggtagggg ttggtgttgg ccaggggatt ttcaatgtcg tgttggggta aaggatgccg
    31561 cagtcaggcg gtaaaccatt caggcttgcg atactttgaa tagatacgcc gaatgacaaa
    31621 gcgatcgaac tgcacaggtc accctctttt accacatact cacgcggagg aaccggtgtc
    31681 ggggttggcc ccagggttgg ctccagggtg gctgtggggg taagcgtgag ggtaggggta
    31741 ttcgtgacgg tgggggtggc ggtaggtgtg gtaacgcgac cggttgtctg aaggatcacg
    31801 aaaattagcg ccgctccaag cccaagaatc aatacaatga gggcgatggc tgccggaagg
    31861 ctgaccttta actcgggtag tttagggttt tgcaccgggc tggtttcggt tttcttcgtt
    31921 gcggcagggg tgaaggtgcg cccgcaaacc aggcagcggg tggcattttc actcaacctt
    31981 gagccacagg ttggacaggt ttttgaagtg gaagatacat tttctggtgt catatgaatt
    32041 atctcttcat ggagcgtaat cggaaaaacc gatcacgggc cgcttccttt gcatcaattc
    32101 aagattgtgt tattctaacc tgaaaattat ggaaatcagt atttattttc acatcccttt
    32161 ttgtaatcgc cgatgcgatt attgtgattt taacacatat gctggcctgg aaagacaaat
    32221 ccctgaatat gtcaaggcgc tcaatcgcga gctggaaatg gttcttaaaa atgccccaac
    32281 ggattttaag gtgaccacca tttactttgg cggcggtacg ccgtcactac tgaacagtac
    32341 ggattacgcg accttattta ccacaatagg gaatagcgca gtggtttcgc cagatgcgga
    32401 aatttctctc gaagccaacc cggattcatt atcggatgaa aaaatcaccg cttatcgaca
    32461 ggtgggtttt aaccgtatca gtatcggcat gcaatcggcg tcaaggttcg atttgaaggc
    32521 gcttggcagg gcgcatacga atcaatctct gctcgcgagt gtggatggat gcagacgagc
    32581 aggctttcaa catattaacc tggatctgat cttcggcatt ccggggcaaa cgcttgagag
    32641 ctggcagcga accctgcggt tggcgcttga attaggcgct gaccatctgt cgttgtattc
    32701 attggtcatt gaggagggta caaggctaag gcgtttattt gataaaggat tgctggttga
    32761 agtgaatgac gacctcgccg cggatatgta tgaaacagcc atggaggagc tcgcacaggc
    32821 tggttacagc cagtatgaaa tttccaactg ggccaaaaat agcgatgcac gctgccggca
    32881 taacctgcag tactggcgtt acgctcctta tctcgggttt ggcgctggag cacatggctt
    32941 ctggggttct accaggaccg agaatttgta cacgattcag gaatatatgc aggctgtgaa
    33001 tttgaaatcc ccggcagctt tccctgcggg atgtgcttgc cagtccacga tcaccatgaa
    33061 ccgttgggaa atgatgcaag aaaacttgat ggtttcatta cgccttacgg aagaaggggt
    33121 atctttatcc aggtttgctg agcgttatgg tgtggaatta atggaagttt ttccccatca
    33181 actggaaaaa accttacgcg aggggttaat agaatacgac gaagaaaatg accgcttgag
    33241 gttaacctac agagggaggc tattcggcaa tcgcgttttc tctcaatttg tttcgaataa
    33301 ggttccgaaa ggatacgaac acctgttggc tcagtagaca tgcacctggg tgccgaagcc
    33361 gatggtggtc attttactca tatcgccttc aggtgtgacc cagcggtaca accaccgcgc
    33421 ttcttcggtt ttcatgttga tacagccatg actcatcggc ataccgaagt tctggtgcca
    33481 gtaagtgccg tgaaaagcga ttccgttttc tggttcgaaa aa
//
LOCUS       DAOSPM010000007        31244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1472_length_31244_cov_14.528007, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000007 DAOSPM010000000
VERSION     DAOSPM010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 31244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31244
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1472_length_31244_cov_14.528007"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /gene="pyrH"
                     /locus_tag="PLW25_01475"
     CDS             complement(<1..584)
                     /gene="pyrH"
                     /locus_tag="PLW25_01475"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOS53189.1"
                     /translation="MEQLRYHRILLKIGGESLAGPSGSGIDAAQAKDIARRLKEVREL
                     GVDIALVIGAGNLWRGKIGLEMGMDQATADYMGMLGTVMNSLALMDALEHQDVVTRVM
                     TAIEMRTVAEPYIRRRAIRHLEKGRVVIFGGGTGNPYFSTDTAAALRAMEIEADVLIK
                     ATKVDGVYDSDPKVNPAAVKFDELSYLDAIQQRLG"
     gene            complement(637..1254)
                     /gene="tsf"
                     /locus_tag="PLW25_01480"
     CDS             complement(637..1254)
                     /gene="tsf"
                     /locus_tag="PLW25_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOS53190.1"
                     /translation="MEITTEMIKSLRESTGAGILDCRKALEQSNGDMNAALDFLREKG
                     LATATKRAARTASEGIVELYNHGDGRVGVMVEVNCETDFVGRSEAFRTLAHEIALQIA
                     ATSPEYVLETDIPESVIDRETKIATAKAKEEGKPEAILPRIVEGALKKYKNEFVLMLQ
                     PYIRDESITVQELINQNVAALGENIVVRRFARWALGETSAEESES"
     gene            complement(1310..2521)
                     /gene="rpsB"
                     /locus_tag="PLW25_01485"
     CDS             complement(1310..2521)
                     /gene="rpsB"
                     /locus_tag="PLW25_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOS53191.1"
                     /translation="MSSIISMKSLLESGVHFGHRTNKWNPLMRPYIFTERNGIHIIDL
                     QQTVKLLTTAYNLVRDTVAQGGFIMFVGTKRQAQETITEEAVRCGMPYVTERWLGGML
                     TNWSTMYSRIQELEKLERLRDSGEIHRLTKKEGLLIEREINRLQARLSGVREMKRVPD
                     LLFIVDVMREHTAVHEANLKGIPVIALVDTNCDPRHVDYVIPSNDDAIRAIKLLVGKI
                     ADAVIEGKSMVEKEESSEAPAAETPAARVAAGVRPRFEEEVELEDADLLGASTLAKIG
                     PKADAAKEDEDLDMKPGVGKSVIAEETLDVLEEEVDKTVSVKSKSAKKSDDEDAAAAA
                     EEEKSTAKGKHARTAKEIVKNIGDFSEDLVDNIKDKIEDTVDDLKDKAEDLIDEIKDK
                     VEDIVDKTDGE"
     gene            complement(2822..3655)
                     /locus_tag="PLW25_01490"
     CDS             complement(2822..3655)
                     /locus_tag="PLW25_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HOS53192.1"
                     /translation="MLKTISRTWILLLLLSGCGVQSTNISPTVYPSSTLSTNVRWWRP
                     TPGLTWQLQFSALPVEMGVEADVYDIDLFENDASVVEALHKQGRKVICYLSVGSREDW
                     RPDAGQFPGEVIGKSYIGWPGERWLDIRRIDLLAPIMRARLDQCKAKGFDGVEPDNIE
                     VFTQDSGFPITYADQLAYAQWLAAEAHARGLAIGLKNAPGMVADSLMFFDFAIIESAF
                     FYEWIEETLPFIEAGKPVFAVEYTDTGVDFQAACDWSRENQISMIQKDRMLTSALIVC
                     P"
     gene            3773..4555
                     /gene="surE"
                     /locus_tag="PLW25_01495"
     CDS             3773..4555
                     /gene="surE"
                     /locus_tag="PLW25_01495"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOS53193.1"
                     /translation="MHILVTNDDGVQAPGLLALATELSSLGEVTILAPDRNWSASGHV
                     KTMHRPLRVKPVLLADGTPALSSDGAPSDCVALALLGVVEKPIDLVVSGINPNANVGH
                     DVTYSGTVTAAMEAAIGGVRGMAVSLDAPEHHKGALDYAAAARVAGFVVNRLLQNTLP
                     VNVLLNVNVPYLKYEEIKGIRVSRQGLRIYRDALIRREDPRGRPYYWIGGEAPTGVPR
                     EGTDFGDLQAGYVSVTPLHLDLTAHEQMAHMQSWDWSADEIR"
     gene            4665..5087
                     /locus_tag="PLW25_01500"
     CDS             4665..5087
                     /locus_tag="PLW25_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOS53194.1"
                     /translation="MDKTTVKDWMKNLVVFVDPDTTVFEGLHIMRHRFATSLIVKKTA
                     ETPDYGIVTSINICDKIIAQGNDPTKTKVKEIMASPVFTVPSSMLITDCAAMMKEKRI
                     HHLPVADEHGQIVGIITAMDLLWMAEAMGTNFKDRGLS"
     gene            5137..6111
                     /locus_tag="PLW25_01505"
     CDS             5137..6111
                     /locus_tag="PLW25_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOS53195.1"
                     /translation="MRRFFILFIRVALLGGLLLSLPQGTAQAATVSPGELINLVNGLR
                     AANGLPALIEDAILDGTAYATAQAMAANGVCAHIGGARERIRAAGFGGGAVVFATENM
                     ACAVSATISDIQSWWSDSLHMMPMNEARYTHVGAGAYTASNGTTYYVLHAAYVGDGGS
                     ASLPGSTGGSAGQGQNPAEPLVEPVVTSTPQADGTIVHTVQYGQALDTIATWYGVTIA
                     EIKALNHMTSNTIYVGDNLIIKIAPTATITPTRTLTPRQPTRTPSMTPTLTKTVMVST
                     PTPTRAPGLLDTLPKIDRQWLGFGLLLVSAAGLLLVLYFLFIRPRFKK"
     gene            6436..7011
                     /locus_tag="PLW25_01510"
     CDS             6436..7011
                     /locus_tag="PLW25_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53196.1"
                     /translation="MEKRNRVTLVLGLLLVLAGTYFVLINAVPGLAQLITVHFSWPVI
                     IMLVGAGLLILGLLSGVAEMSIPAFIVAGIGGILYYQNLSGNWSSWSYLWTLIPGFSG
                     LGVLVAALFGARKKGAARDGLDAIGTSLVLLVIFGAIFGAFKPLGPYWPVLLIAAGLV
                     IGARAFIKGRKEETPVNVIDVKPSGEADENM"
     gene            7020..7661
                     /locus_tag="PLW25_01515"
     CDS             7020..7661
                     /locus_tag="PLW25_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53197.1"
                     /translation="MSQQSRRILLGVLLVVAGAAFLLQQLLEIPIGGLFIAFLFVLAG
                     CVFLYFLLRDRHKWWLAIPGFTLIGIGGVILIKKLLPNLAGDYGGAFFLLMVALGFLV
                     VFLLNQAQWWPVIPAGILTTLALVSALDLNGMANGGLLFLGIGATFTVLGLMPVGKAE
                     KWPWIPAGICLLLGLILFASSGALMGSAVGWVFALVLIAAGAYFVIRSLIKKA"
     gene            7672..8127
                     /locus_tag="PLW25_01520"
     CDS             7672..8127
                     /locus_tag="PLW25_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOS53198.1"
                     /translation="MQNRLLRSKQDRILGGVCAGLARYLNIDTVLVRLFFIVFTLVGG
                     IGPLLYIILWIVVPEDQAESSSGYSFNGEVLRERAENFADEFVNAVQKPGNKASFYVG
                     IGLVLLGVYIFLKNLNLPWLAWLNTSVILALLIIIAGAALLYVALKRKT"
     gene            8215..8934
                     /locus_tag="PLW25_01525"
     CDS             8215..8934
                     /locus_tag="PLW25_01525"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOS53199.1"
                     /translation="MKKSLLVIVASIMAVMSLAACAGSGQPAGMPNLRTLSASGSGEV
                     YLVPDVAYINVGIRVDADEVSDALSKNNAQANSISEVLQQLGVDKKDVQTSNFNVYPM
                     MDYGIDGQVSRRYYVVENTVYITVRDLTSLGKLLDAVVRSGANTINGISFDVLDKTAA
                     EAKARDMAIANAKAEAEAIAASAGVKLGNLQSVNVYTGGIAVPMYDAKGIGGAAMDSV
                     VPISAGQLKINVEASVIYEIK"
     gene            complement(8998..9987)
                     /gene="glpX"
                     /locus_tag="PLW25_01530"
     CDS             complement(8998..9987)
                     /gene="glpX"
                     /locus_tag="PLW25_01530"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOS53200.1"
                     /translation="MPNTTPTRNLAFDLVRVTEAAALAAGRMMGRGDKNGADKAAVDA
                     MRVMLNSISMRGTIIIGEGEKDEAPMLYNGEKLGTGEGPEVDIAVDPIEGTRPLAYGL
                     WNSISTVALAPRGTMFNPGPFVYMNKIAVGPVARHAVDIDAPVSDNLHKVAKAKGVKV
                     EDLTVVVLDRPRHQELITQIRMAGARIKLIPDGDVAGALMTSMGDNTADLLMGVGGTP
                     EGVLAAAGLRCMGGEIIGKLYPRNQRELDLAKEMGVDFEKPLTMDDLISSDNVFFAAT
                     GITTGDYLRGVTYFKDGARTDSVVMRGLSGTVRRVISTHKLSKLMRISSIKYK"
     gene            complement(10018..10842)
                     /locus_tag="PLW25_01535"
     CDS             complement(10018..10842)
                     /locus_tag="PLW25_01535"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOS53201.1"
                     /translation="MKTDLSLRKRLIVINLMVPTLIVFSILGGWAFTVFVAVVLLLAA
                     WELWRLFNKGQYSPSLLVMGIFVPLAVVLRHLYAFSYSDVWLAALVLTSMLFHVLQQN
                     RGGMTAAIDLMITIGGTVYLGWLGSYAISLRALENGLHWVVLVFPIISLADTGAYIFG
                     RLLGKHKMLPRVSPKKTWEGYLGGILVGMLGGWALAALWHKPAPLILPWYGLVLGAVI
                     SILAPFGDFGESMLKRQFNAKDSGNFLLEHGGFLDRMDSSLWAAAIGYYVIILLTR"
     gene            complement(10832..11557)
                     /locus_tag="PLW25_01540"
     CDS             complement(10832..11557)
                     /locus_tag="PLW25_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOS53202.1"
                     /translation="MTKKTPAKIPAHIAIIMDGNGRWAKAHGLPRLEGHRAGTENLRQ
                     IIKGCAEIGVQHLTLYAFSTENWSRPKTEVDGLLTILETYLDKEVDELCAEGVRLHHI
                     GRLEAMPESVRKKVEHSMEVTCSNTRLILHLAWNYGGRDEIVHAIQKMIRDGIPSKDV
                     TEELVDRYLFTAGTPDPDLIIRTSGEMRVSNFLIWQGAYAEWYVTPTLWPDFTKEELF
                     KAIEIYDQRDRRFGNISSSNNEN"
     gene            complement(11575..12132)
                     /gene="frr"
                     /locus_tag="PLW25_01545"
     CDS             complement(11575..12132)
                     /gene="frr"
                     /locus_tag="PLW25_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOS53203.1"
                     /translation="MLKEITKDAENRMKSALLSLEEDLTAIRTGRATPALVEKLPIDY
                     YGAPTPLLQLASISVPEARALLIKPFDPSTIKTIERSILASELGLTPNNDGKSIRLNL
                     PPLTEERRRELVKVVNNRLEECRVAIRNVRRDLIKDLRDFEKEKMISKDDLETGEQDL
                     QKLTDLMIDEIELIGKRKESEIMEI"
     gene            12344..12577
                     /locus_tag="PLW25_01550"
     CDS             12344..12577
                     /locus_tag="PLW25_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53204.1"
                     /translation="MNTGMLWFDNDPKADFLTKINRATDYYLKKYGQRPDLCFVNPGM
                     KFEPPPKNVGIEVQTDQMIMPDHFWLGIKTCSA"
     gene            complement(12622..13560)
                     /locus_tag="PLW25_01555"
     CDS             complement(12622..13560)
                     /locus_tag="PLW25_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HOS53205.1"
                     /translation="MMTEEDIDSGRLILQSRHDFSHAYQKGFWRSVFHLIQKKNNELL
                     PFEEVLKHIPMRGQNYIGVRQIETEKIIGSVSRYNDFDRAFLPRQTHTRSRWESIDRA
                     HFKQIILPPIEVYKVGDFYFVKDGNHRVSVAREKGQVYVDALVTEVHTSGKITRDTDV
                     NALVLEQEYSEFLRQTRLNEFYPGEDFRFSIPGQYEKVLQHISVHRWFMGEKLTRPVT
                     DEEAVKGWHREVYLPLVRIIRKHQILNAFPARTEMDLYFWIIEHRWYLAEEQKRRISL
                     ESAATDFTNRFSQRPFRHVSQLWQRLKKRIFNQEKK"
     gene            complement(13564..14223)
                     /locus_tag="PLW25_01560"
     CDS             complement(13564..14223)
                     /locus_tag="PLW25_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS53206.1"
                     /translation="MKILAVSDVENALLYSPMIMDRFQDVDLILSCGDLPYYYIEYMV
                     SMLNVPCYFVRGNHANAREYGVAGTRKYPWGAVDVHHRCVEDDTGLLLAGLEGSLRYN
                     QGPYQYTQGQYWQMVLSLVPRFFWNKLALGRYLDIFMAHAPPWQVHDEEDLPHRGIKA
                     FRWLIKVFQPAYFLHGHIHIYRRETITRTLIGGTTVQNVYGYKTLHIPAPRPRSWRGQ
                     R"
     gene            complement(14239..14616)
                     /gene="acpS"
                     /locus_tag="PLW25_01565"
     CDS             complement(14239..14616)
                     /gene="acpS"
                     /locus_tag="PLW25_01565"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOS53207.1"
                     /translation="MMEVACGIDLVEVERLRSITPAIKARFVSRVFTTQEIEDSGGRD
                     DRLAARFAIKEATVKALGCGIGEVGWQDIETQLDENGKPVLRLYGAAAQMAERMNWRS
                     WSVSISHTRNLAAACVTALVEKR"
     gene            complement(14613..15917)
                     /locus_tag="PLW25_01570"
     CDS             complement(14613..15917)
                     /locus_tag="PLW25_01570"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOS53208.1"
                     /translation="MIPKYTLSSSIPGPADITRQVLRNGIVLLTRSNFNSSSVVISGY
                     VSAGSQLDPLEKLGLAHFTALALMRGTESSDFQSLYDRLESAGASLGFGASVHTTSFS
                     GRALAEDLPLLFATLAECLSSPVFPSEQVSRLRAQLLTGLAIRGQSTRDTAALEFDSL
                     LFPDHPYGRPEDGYTETIQNITRDDLADFHRQQYGPEGMKLAVVGAVSADRVFELAEK
                     HFGNWLTGHKRQTCSFKAIKPPQASITRKVIIPGKSQTDLIIGTLGPKRKDPEYLAAS
                     LGNSILGQFGLMGRVGDAVREKAGLAYYASASLNAWIESGSWEVSAGVSPGNVEKATA
                     LIIEELRRFTTEPVSQEELEDNQNNYIGRMPLSLESNAGVANSILNLERFELGLDYLQ
                     RYPTLVSALTREHILAAARKFINPDKLIIVSAGADGKVNNNP"
     gene            complement(15914..17200)
                     /locus_tag="PLW25_01575"
     CDS             complement(15914..17200)
                     /locus_tag="PLW25_01575"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOS53209.1"
                     /translation="MTETITQVELENGLKVLMKEIHTAPIISQWVWYRVGSRFEKPGF
                     TGISHWVEHMQFKGTAKFPAGVLDKAISRDGGYWNAFTYLDWTTFFETTPANKIDLAM
                     TLEADRMVNSLFDENEVNSERTVVISEREGNENEPLFLLDEAVQFAAFDHHPYKNEVI
                     GSIADLRSITRDDLYAHYRKYYNPGNAVLALAGDFDSEKILERIRQTYQPLHGHSVPE
                     PEYLREQLLAEEKRIEVAGPGETTYLQVAWRSPAATEAEFFAFTIIDSLLTGPTSLNM
                     FGGGGISNKTSRLYRRLVEKEYAISLNGSLQATHDPFLYTITITVHPKHQPEVVLEQL
                     DKELDVLRQAPVKQTDIDRAAKQARALFSYGSENITNQAFWLGYSEMFDQYSWFTRYT
                     SRLAEVTPEILLDTARKYLNQNQRVVGIYRPDGAKR"
     gene            17335..18663
                     /locus_tag="PLW25_01580"
     CDS             17335..18663
                     /locus_tag="PLW25_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS53210.1"
                     /translation="MSKRIQVAIIGAGPAGLFAAEKLTQADIAVALFNRDIKPGGMAE
                     YGIYPEKHQLKDGLRKQFERILSYEQVHYFGNTCVGEDQSLTIPRLLEWGFSAVLICC
                     GAQGTKWLGIPGENLEGVIHSKNLVFHYNRLPPYCTAPIKIGKQAVVVGAGNVMADVT
                     RYLLGLPQMEKIHVCVRRGPAEVKFTAKELESIIGGMDMDALEHEFERVKPVMVPLGQ
                     DPEVEKAVYLKAFGKAGKTGLKPLIEMHFLVSPVKIEGDEQGGVNTVVLEENTLVLSG
                     EKTSARGLGTHRSLAADTVVFAIGDRVEERLGVPVSGNEYRHVENPRYPVEGQSYEVE
                     APGSGQAWKGVFVAGWSRLASSGLVGNAKKDGVNGAEAVEQYLREERPGGGLLLDELE
                     SRLSSLPDPVVQQRHLARLLAEERKRAGGGPIEYAKFTTNAEMLQVMGLQ"
     gene            complement(18674..19159)
                     /locus_tag="PLW25_01585"
     CDS             complement(18674..19159)
                     /locus_tag="PLW25_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53211.1"
                     /translation="MTENSAIENEGKPKVTANSLPALPLARIHFALLVMLVGFIIFLV
                     GARPAIFGLDRSPVVGFIQIAVMLIGIMIICLGGYTSVRALWRHQPPSISADIGMRLV
                     STGYVIVTFSGLADVFGVGTHPLPGVPYFGVWQARGMEIGCAIIAIGFILSFPPASKP
                     Q"
     gene            complement(19297..20562)
                     /locus_tag="PLW25_01590"
     CDS             complement(19297..20562)
                     /locus_tag="PLW25_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOS53212.1"
                     /translation="MDQNVTHNGSGANLDNNQNDNNMASLLEKEGLGIDFPTQGEIRK
                     GIIASITPGQILVSVGTKSEGIITGKEYELIPSDELANLKVGDEISVYVVNPEDQNGN
                     LVLSYMRAREEESWQEVEALQASKQATHSTILGYNKGGLIVPIGGLRGFVPASQISLT
                     RRAGVTGDTPEQRWGKMIGQEIDVCVIEVDRERRRLILSERQASTETRESVKERVLEE
                     LKEGEVYLGRITSLADFGVFVNVNGADGLVHMSEISWERIQHPSEILKVGQEVKVKVI
                     NIDHEKKRIGLSIRQLQSDPWDQKAAQFQVGQLIEGTITRLTKFGAFARLAEDIEGLI
                     HISEISERRIEHPKEVLKEGDICTLRIIKIDPSVHRIGLSLRRVDSMAYADMDWQALE
                     GILDEDTAVIAKDDDQSTAEEPTEPAPEA"
     gene            complement(20717..>21154)
                     /locus_tag="PLW25_01595"
     CDS             complement(20717..>21154)
                     /locus_tag="PLW25_01595"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53213.1"
                     /translation="EVGDAAFLSLQTAQPDDLSAEARTAVEKTLGRRVQNQIYRHILL
                     SVISELWVDYLTRVDALRVSVGLEAYAQRDPLVQYKGQASELFKNLLSDIRGGVISRM
                     FLFQPRRPGGQPAERSAPEGASPAAQTQIPPAQSSPKKKRKRH"
     assembly_gap    21155..21254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21255..24327)
                     /locus_tag="PLW25_01600"
     CDS             complement(<21255..24327)
                     /locus_tag="PLW25_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53214.1"
                     /translation="MVLQKIARVFGGDPHKREIEHLSQIVDEINQLETVFEGLSSDAL
                     NHKTSEFKQRLAEGESLDDLLPEAFAAVREASKRTLGLRHYDIQMIGGMTLHHGKIAE
                     MRTGEGKTLVATLPLYLNALTGGSVHLVTVNDYLARRDARWMAPVYNLLGLKVGVLQM
                     AARTENGRKAFLVDLEKPNAREDLHMLALVDRGEAYKADIVYGTNSEFGFDYLRDNLT
                     MSLEERVQRGHYYAIVDEVDNILIDEARTPLIISGPASDETEDYVRMAQVVRALNPED
                     IEVNEKDHQVSLTEIGDAHVEEILNQPLRNPDRPEDIAPEQARLLGYLEQALRAQHLF
                     RRNKDYIVQGGKVIIVDEFTGRLMPGRRWSEGLHQAVEAKEGVKVEPENVTYATITIQ
                     NYFRMYEKLAGMTGTALTEAEEFSKIYKLEVLPIPMNLEYVAAQQDSPLTVVETRDRQ
                     NYPLQYYSKKDDPHHEAVFWRRQDFPDLVYRTEEGKLRAIVAEIIHYYAIGRPQLVGT
                     TSVEHSERLSQRLSAESVRRLAQTLVIRDAWLEKFNKSAEIEITELQFLNRPLHELNV
                     AEMRPLARQAGLSSINPEDPDLLERLLKVMDLEVPHKERVLEVLKGGVPHQVLNARKH
                     DEESQIIAKAGAFGSVTIATNMAGRGVDIKLGGELNEEVLADVIRVLEKAGVADPYDL
                     NNDTRRGELEKLTLEDYGIYEESVNTFIRYLEDMKRVRALGGLHVIGSERHEARRIDN
                     QLRGRAARQGDPGSSRFYLSLEDELMRLFGGAQMEGLMTRMKVDPSLPIESGLLGRMV
                     EQAQERVEGNNFDVRKHLLEYDDVLNSQRKRIYAERNRAFEKESLHEDVLEILRTEIN
                     ERIPAALKDEEGPWKLLSYLEEVQPSMVFEAEGIRTPSYTIRLIVDELQRRINGQTDD
                     SNIKDQIIEITEKALQAENEHILNATSLLLQKTEETIDQLTAERLESVDAIFDGLGSS
                     HGEEEAPRRPQEILEELVNLTRTQLRLTTEEMRALPEGDDEVKEKVNNQVKR"
     gene            complement(24377..24655)
                     /locus_tag="PLW25_01605"
     CDS             complement(24377..24655)
                     /locus_tag="PLW25_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53215.1"
                     /translation="MYTDQDITNLVTRVALIEKELEKPPASRQSMPELGILSSNFLTR
                     AFSVWGLYFVANLLIGIVFGCIFVAIGLALGASFTDILQHILKGVPLS"
     gene            complement(24724..26121)
                     /gene="lpdA"
                     /locus_tag="PLW25_01610"
     CDS             complement(24724..26121)
                     /gene="lpdA"
                     /locus_tag="PLW25_01610"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOS53216.1"
                     /translation="MEEYDVIVIGAGPGGYTAAIRSAQLGKKTAIIDRRWLGGVCLNV
                     GCIPSKSLLKNADVALTLRERGREFGFSVENLQLDFTSAVKRSRQVSDRLTRGVAYLM
                     KKNNITVYMGEARFSSRESVTLTGKDGTAQELKARDFIIATGSTPFSLPGVNIDGVKV
                     LTYQEAILQEKLPQSVVIIGAGAIGLEFATIWNAYGSKVTLVEMLPRIAPLEDEEISA
                     ELAKTFSKRGITCLTETRVLAVETTPGGVRVSTSGQNGESVIEAEQALVAIGFKPNSQ
                     NLGLEEIGVELTERKHIRVNDQLTTSVPNIWAVGDVTGKLLLAHVASAQGIRCAEALA
                     GLAVTPLDYTMIPRATYSHPQVASFGYTETQARALGKTVKIGKFPFQPNGKALGLGDY
                     GGFAKLVVDEHSGHLLGAHLIGPEVSELLPELTLAQLNGLTAGDIARNVHAHPTLSEV
                     LMEAAEMVEGKAIHI"
     gene            complement(26263..>26955)
                     /locus_tag="PLW25_01615"
     CDS             complement(26263..>26955)
                     /locus_tag="PLW25_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS53217.1"
                     /translation="YNEEKTISQVLDLVLEKVRNLGEVIIIDDGSKDNTGQISRDYEA
                     RNPLVHYYQQPRNMGKTEALKEGFKRSNGEIVIVQDADLEYDPAEINDVVAPIQEDKA
                     DVVLGSRFLVRRASRSLYFRHYMANRLITFLSNLFTDLNLTDVETCYKAFRGDIIRNM
                     VITSTGFGFEVEVVAKVAKLKCRVYEVPISYYGRTYEEGKKINATDGLMALWYIIKYN
                     VFTSKKSSFRKS"
     assembly_gap    26956..27055
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27056..27828)
                     /gene="folP"
                     /locus_tag="PLW25_01620"
     CDS             complement(<27056..27828)
                     /gene="folP"
                     /locus_tag="PLW25_01620"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOS53218.1"
                     /translation="MRQQTLPEFGKKTYVMGILNVTPDSFSGDGLLSQPDTVRAALEQ
                     AQRFIEAGADILDIGGESTRPGAEAVNEAEELARVIPVIEAIRSSGGEVLLSIDTYKA
                     PVAAAALEKGADWINDVWGLRADPLIAATAARYQAPIILMHNRSKPANAKLQQRLGGR
                     YIGIEYANLLQDICAELLESVTLARAAGVDDRRIILDPGIGFGKTVEQNLELVNRLGE
                     IKALGYPLLLGASRKSFIGYTLDLPPVERVEGTAAVCAIG"
     gene            complement(27855..28346)
                     /gene="folK"
                     /locus_tag="PLW25_01625"
     CDS             complement(27855..28346)
                     /gene="folK"
                     /locus_tag="PLW25_01625"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOS53219.1"
                     /translation="MAKKETVYIALGSNLGDRAEFLYRARQLLSEFITEMRCSRLYET
                     PPWGVLEQPLFLNQVLRGDTTLPPLKLLDSLKNIEKHLGRKATLRFGPRVIDLDILLY
                     GERVIRSARLQVPHPRMLERAFVMLPLAELSPELIIPGSGKSVNQVLSSLDTTGISLY
                     PSS"
     assembly_gap    28479..28578
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28579..29194)
                     /locus_tag="PLW25_01630"
     CDS             complement(<28579..29194)
                     /locus_tag="PLW25_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOS53220.1"
                     /translation="MEVIRVALVGFGHVGQALMALLESKQERLRAEFGFSTQVTGIAT
                     GHHGVAIDPEGIDTPQALRCAQKGQAISALSAVPAPASVPDFIASVPADVLFENSPVN
                     HQSGQPAASYLRAALERGMHAITANKGPVVHAYTELTRLAAQKQRRFLFESAVMDGGP
                     IFSLFRGALPAVELQGFQGILNSCTNLLLEMMESGRTFEEAVAYG"
     gene            complement(29223..30050)
                     /gene="murI"
                     /locus_tag="PLW25_01635"
     CDS             complement(29223..30050)
                     /gene="murI"
                     /locus_tag="PLW25_01635"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOS53221.1"
                     /translation="MNKSQHLVGVFDSGVGGLSVLREILTALPAQPVIYFADQAHVPY
                     GGRQLTEVQAFARGITQYLLRQGVELIVVACNTASVAALHALRQEFPAIPFVGMEPAV
                     KPAVEQSRSGKVGVLATEATFHTSMYASVVERFGQGITLFEDPCPGLVAQVEKGDLNG
                     PATRRILESALTPMMEKGVDTIVMGCTHYPFVIPLIQQIVGEEVRVIDPAPAIARQLR
                     RMLEAHNLLVEGQEVSVPRFITSGNPIQFQDALYLLLQQKHPVGRVFWRDGEVCERA"
     gene            complement(30067..30786)
                     /locus_tag="PLW25_01640"
     CDS             complement(30067..30786)
                     /locus_tag="PLW25_01640"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOS53222.1"
                     /translation="MAPPLIGITVSQISRSHKRKANMLLSEYTNAVHLAGGCPVLIPN
                     EYPLDRLQVLRGVLQGILISGGGDIDPMLYQARPDPYANSIVKERDALEKALVELAVK
                     TDWPLLGICRGLQMLNTALGGTLFTDIPRQYDTSITHNQPNDVPPNKLIHPVTIEPAS
                     QLGRATACSELRVNSRHHQAVRETGIGLQVTAQASDGLIEAIEHPGQRFCLGVQWHPE
                     SLQAMEEHRAIFRAFLHAASQ"
     gene            complement(30868..31215)
                     /gene="rplS"
                     /locus_tag="PLW25_01645"
     CDS             complement(30868..31215)
                     /gene="rplS"
                     /locus_tag="PLW25_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOS53223.1"
                     /translation="MSDILKAYDAPVNPNIPSLRTGDMVSVHVKIKEGNRERIQEFKG
                     VVLYQHNSGNDSTFTVRRVASNGIGVERTFLLRSPRIEKVVVERHSKVRRSRLYFLRD
                     RSGKSARLKQKFN"
BASE COUNT         6669 a         8296 c         8360 g         7619 t
ORIGIN      
        1 cccaaccgtt gctggatggc gtcaaggtag gagagctcgt cgaatttcac cgctgcgggg
       61 ttgaccttgg ggtcagaatc gtacacccca tccaccttgg tggctttgat gagcacatct
      121 gcttcaattt ccatcgcgcg cagtgcggca gcggtatcgg ttgagaaata cgggttgcct
      181 gtacccccgc caaagatgac cacacgtcct ttttccaggt ggcggatggc gcggcggcgg
      241 atatacggct cggcaacggt tcgcatttca atagcggtca tcacacgtgt cactacatcc
      301 tgatgttcca gcgcgtccat taaggccagg gagttcatca cggtgcccag catacccata
      361 tagtctgcgg tcgcctggtc catgcccatt tccagcccga tcttgccgcg ccacaagttg
      421 ccggcgccaa tcaccagcgc aatatccacg ccgagttcgc gcacttcttt caacctgcgg
      481 gcgatatcct tcgcctgggc ggcatcaatg ccgctgcctg agggacccgc taaagattcg
      541 ccgccaatct ttaatagaat tctgtgatat ctgagttgct ccataatacc tcctgaacgt
      601 tagaaaaaga ccaacccgaa ggctggtctt tgaaggtcac gactcgcttt cttcagcgct
      661 tgtttcgccg agcgcccaac gggcgaagcg gcgcacgacg atattctcac ccagcgcagc
      721 gacattctgg ttgatcagtt cctgcacggt gatgctctca tcacggatat acggctggag
      781 catgagcacg aattcgtttt tgtatttctt taacgcgcct tcaacgatgc gcggcaggat
      841 ggcctcaggc ttgccttctt ctttggcttt ggcggtggcg atttttgttt cgcgatcgat
      901 cactgattcg gggatatcgg tttccagcac atactcaggg gaagtggctg caatttgcag
      961 cgcgatttcg tgcgccaggg tacgaaacgc ttcagagcgg cccacgaaat cagtttcgca
     1021 gttcacttct accatcacac ccacgcgccc atccccgtga ttgtagagtt ccacaatgcc
     1081 ttcagaggct gttcgcgcgg cgcgcttggt ggcggtagcc agcccttttt cgcgcagaaa
     1141 atccaaagcg gcgttcatgt caccattgga ttgttccagc gctttgcggc aatcgaggat
     1201 gccagcgccg gtgctctcgc gcaacgattt gatcatttca gtggtgattt ccattttaca
     1261 gattccttct tctccaggtg gtcaccggtc agagaccgat gttgattgac tattcaccgt
     1321 ccgttttatc cacgatatct tcaactttgt ccttgatctc atcgatgaga tcttctgctt
     1381 tgtccttaag atcatctacg gtatcctcga tcttgtcttt aatattatcg acaagatctt
     1441 cagagaaatc cccgatgttt ttcacaattt ctttggcggt tctggcgtgc ttgcctttgg
     1501 cagtgctctt ttcttcttca gccgcggctg cagcgtcttc atcatcgctt ttcttcgctg
     1561 atttggattt tacagacacc gtcttatcca cttcttcttc aaggacatcc agcgtttcct
     1621 cagcgataac cgatttacca acgccgggtt tcatgtcgag gtcttcatct tccttcgctg
     1681 catcggcttt tggcccaatt tttgccaaag tggaagcgcc aagcagatca gcgtcttcca
     1741 gttcaacctc ttcttcgaag cgcgggcgga cgccagcggc tactcgggct gccggggttt
     1801 cagccgccgg ggcttcactg ctttcttctt tctcgaccat tgacttgcct tcgatgacgg
     1861 catcagcgat cttcccaacc aacaatttaa tggcgcggat ggcgtcgtca ttggagggga
     1921 taacgtaatc cacatgtcgc ggatcgcagt tggtgtctac cagggcaatc accggtatgc
     1981 ctttcaaatt ggcttcgtgc acggcggtgt gctcgcgcat cacatctaca ataaaaagca
     2041 ggtctggaac gcgcttcatt tcgcgtacac cagaaaggcg cgcctgcagg cgattgatct
     2101 cgcgctcaat caacaggcct tcctttttgg tcaggcggtg aatttctcca gaatcacgta
     2161 aacgttcaag tttttccagt tcctggatgc ggctgtacat ggtgctccag ttggtgagca
     2221 tgccgccaag ccagcgctcg gtgacataag gcatgccgca ccgaacggct tcttcagtga
     2281 tggtttcttg tgcctggcgc ttggtgccaa cgaacatgat aaaaccaccc tgggcgactg
     2341 tgtcgcgcac caggttataa gcggttgtga gaagcttgac tgtctgctgc aggtcaatga
     2401 tgtggatgcc gttgcgctcg gtaaaaatgt acgggcgcat caacgggttc cacttatttg
     2461 tgcgatgccc aaaatggacg ccgctttcca acagggattt catggaaatg atggaactca
     2521 tggggtatta ctcctttgcg tttgcctagc gccttcttta gcctttccct cactgcttgc
     2581 ggcagcacgc aggaaagatc ggaaagcgcg tgtgatagtc tgaggttttc accacagccg
     2641 acgaattata acatgaaatt tcgcgcgaac ttcttttttg gtctcttttt gctgacactt
     2701 ctttttggat tttcaaaatt atcctggata cccggcgctt gtttacatca tcaaccgcaa
     2761 ttccacaaaa tgtcggcact gattcccccg cggttttcct gcctgtcgag cgggtggtct
     2821 cttacgggca gacgatgagg gcggaggtga gcattcgatc cttttgaatc atactgatct
     2881 ggttctcacg gctccaatcg caggcagctt ggaaatcaac gcctgtgtct gtgtattcta
     2941 cggcaaatac aggttttccc gcttcgataa aaggcagcgt ttcttcgatc cattcgtaaa
     3001 aaaacgcgct ttcgatgatg gcgaaatcaa aaaacatgag tgaatctgct accatgccag
     3061 gagcgttctt cagcccgatg gcgagaccgc gggcgtgcgc ttcggcagca agccattgag
     3121 cgtaagccaa ctggtccgcg taggttattg ggaagccgct gtcctgtgta aatacttcga
     3181 tgttatcagg ttccacgccg tcaaacccct tggctttaca ctggtccagg cgggcgcgca
     3241 tgattggcgc cagaaggtcg atacggcgga tgtccagcca tctttcgcct ggccagccga
     3301 tatagctttt accgataacc tcgccaggga actggccggc atcggggcgc cagtcttctc
     3361 ttgaacccac cgagagataa caaatcacct tgcgaccctg cttgtgtaat gcttcaacca
     3421 ctgacgcgtc attctcaaac aggtcaatat catatacatc cgcttccaca cccatttcta
     3481 caggcaaagc agaaaactgc aattgccagg tgaggccagg tgtgggtcgc caccaacgga
     3541 cgtttgtgga gagcgtggag cttggataca cggttggact gatattggtg gattgcacgc
     3601 cgcaaccgga gagcaacaag agcaaaatcc aagttcgtga aatcgttttc aacatatgag
     3661 atgaattata aaccgccgtg agcgtttggt tctctgtggt gggaagatgg gcgcgtcacg
     3721 ggagaggcat acatgtataa ttcaatcaat ctgaaaaaga caggaaacga caatgcatat
     3781 tttggtcacg aatgatgatg gcgtacaggc gcccgggtta ctggcgctgg ccacggaact
     3841 cagttcgttg ggtgaggtca ccatcctcgc ccctgaccgt aactggtcag cttcagggca
     3901 cgtgaagacg atgcaccgac cgctgcgcgt gaaaccagtg ctgctggctg atggcacacc
     3961 tgcgctcagc agcgatgggg cgccatcaga ttgcgtggct ttggccttgc tgggtgtggt
     4021 tgaaaaaccg atcgacctgg tggtcagtgg catcaacccc aatgccaatg ttggccatga
     4081 tgtgacttac tcgggcaccg tgacggcggc catggaagcg gcaatcggtg gcgtgcgtgg
     4141 aatggcagtt tctttagacg cgccggagca tcacaagggg gcgctggatt atgcggcagc
     4201 ggcccgtgtt gccggctttg tggtgaaccg gttgctgcaa aacacactgc cagtgaatgt
     4261 gttattgaac gtcaatgtcc cttaccttaa atatgaagaa atcaaaggga tccgcgtttc
     4321 gcgccagggt ctgcgtatat accgcgacgc gctgatccgc cgcgaagacc cgcgcggtag
     4381 accatattac tggattggcg gtgaagcacc tactggcgtt ccgcgagaag ggacggattt
     4441 tggcgacctg caagccggtt atgtttccgt tacacctctc catttagacc tgactgccca
     4501 tgagcaaatg gcgcatatgc aatcgtggga ctggagcgct gatgaaatac gctaatatgt
     4561 ctgacaatgc gcatttgaca taattagcag gatagactac attaatcata gatggttcgt
     4621 aacttcactt catttcggcg ccatggttgc gcgaggagag aggcatggac aaaacaactg
     4681 tcaaggactg gatgaagaac ctggtggtgt ttgtggaccc ggataccacg gtttttgaag
     4741 ggctgcatat tatgcgccat cggttcgcta ccagcctgat agtcaaaaaa acagctgaaa
     4801 cccctgatta cggcattgtc acttctatca atatctgcga taagatcatt gcccagggca
     4861 acgatcccac caagaccaag gtaaaagaga taatggcatc accggttttc actgtgccgt
     4921 cttccatgct gatcactgac tgcgcggcga tgatgaagga aaaacgcatt catcatttgc
     4981 cggtcgcgga tgagcatgga cagattgtcg gcattatcac cgccatggac ctgctgtgga
     5041 tggcagaggc catgggaacg aacttcaaag accgcggact tagctgagca tcttgacaaa
     5101 aaaacatccc ggcgcatcgg ttataattag ccgttaatgc gccggttttt tatcttgttc
     5161 attcgcgttg ctttattggg cgggctgctg ttatccctgc cccagggaac tgcgcaggct
     5221 gccacggtat caccggggga acttatcaac ctggtgaatg gtctgcgcgc cgccaacggg
     5281 ctccccgcac ttattgaaga tgccatcctg gatggtacag catacgccac ggcgcaggcg
     5341 atggcagcca acggtgtttg cgcgcatatc ggcggtgcga gagagcgcat tcgcgcggct
     5401 gggtttggcg ggggcgctgt cgtgttcgct actgaaaata tggcctgcgc tgtgagcgcg
     5461 acgatttccg atatccaatc ttggtggtca gacagcctgc atatgatgcc aatgaatgaa
     5521 gcgcgctata cccatgtggg agcaggggct tataccgctt cgaatggcac cacctattac
     5581 gtgttgcacg cggcgtatgt gggtgatggg ggaagcgcat ccttacctgg ttctacaggc
     5641 ggcagcgctg ggcagggaca aaaccctgcc gaaccactgg ttgaaccggt ggtcacctcg
     5701 actccccagg cagacggcac gattgtgcac actgtgcagt acgggcaggc gttggatact
     5761 atcgccacct ggtatggcgt caccatcgcg gaaatcaaag cgttgaacca catgacttcc
     5821 aataccattt acgtcggcga caacctcatt atcaaaatag cgcccactgc caccatcacc
     5881 cccacgcgaa ctttaacgcc ccggcaaccg acgcgtactc cttccatgac gcccaccctc
     5941 acaaaaactg ttatggtgag cacacccacg cccaccaggg cacctggttt gctggatact
     6001 ttgcccaaaa ttgaccgcca gtggctgggt tttggtttat tgctggtgag cgcagcgggg
     6061 ctattgctgg tgctttactt cctgttcatc cgccccaggt ttaaaaaata actctcacct
     6121 tctgatccgg tcatttttac cccacagctt cctgtttctt tgtgaaccaa cagattcttt
     6181 tctgctggaa agggacgttc cgccctgctc accaggggat ggatcggtct gagcctctta
     6241 cgcgaatacc gacggacgcg ttaaagaaaa acgcgctgct cgaagccctt tgttttccgc
     6301 gctgattgcg tgccaacatt tcatcaggtt ggtgtcagca tccctgctcc atgtttgcct
     6361 ttggtcgtgc aaccctgcga attcatttgc gtatatggga taaatgggta ttaatgactt
     6421 tcactggagg tagtgatgga aaaaagaaac agggtgacac tggtattggg cttgttgctc
     6481 gtattggcag gcacctattt tgtactgatc aatgccgtgc cgggtttggc acaactgatt
     6541 actgtccact tctcctggcc ggtgatcatc atgcttgtcg gcgccgggct gctcattttg
     6601 gggctgctta gcggagtcgc ggaaatgtcc ataccagcgt tcatcgtcgc cggcattggt
     6661 ggaatcctgt attaccagaa cctcagcggc aactggagca gttggtcgta tttgtggaca
     6721 ttaatcccgg gtttttcagg gctgggagtg ctggttgctg cattgtttgg cgcgcgtaaa
     6781 aaaggcgcgg ctcgcgacgg gttggatgcc atcggtacca gcctggtact gctggtgatt
     6841 tttggcgcca tcttcggggc attcaaacca cttgggccat attggccagt gcttctcatc
     6901 gcggctggcc tggtgattgg cgcacgcgcg ttcatcaaag ggcgcaaaga agaaacacct
     6961 gtaaacgtca tcgatgtaaa gccatcaggc gaagcagatg aaaacatgta ggaggtaaca
     7021 tgtctcagca atccaggcga atcttactgg gggtgttgtt ggttgtggct ggcgcggcct
     7081 ttttgctgca acagctgctg gaaatcccca tcggcggact gtttatcgct ttcctgttcg
     7141 tgctggcggg ttgtgttttt ctgtatttcc tgctgcgtga tcgccataag tggtggctgg
     7201 ccatcccggg gttcaccctc attggcattg gtggtgttat cttgataaaa aagctcctgc
     7261 cgaacctggc gggcgattat ggcggcgctt tctttctctt aatggtggcg ttgggctttc
     7321 tggtggtctt tttgctcaac caggcccaat ggtggccggt catccccgcg ggaattctga
     7381 ccacattggc gctggtctcc gcgctggact tgaatgggat ggccaacggc gggctgttgt
     7441 tcctggggat aggcgcgact ttcaccgtgc tcgggctgat gccggtgggg aaggctgaaa
     7501 aatggccctg gatcccggcg ggcatctgcc tgctgttggg attgatcttg ttcgccagtt
     7561 ctggcgcgct gatgggttcg gccgtgggct gggtgttcgc tttggttctc atcgcggcgg
     7621 gggcgtactt cgtcattcgc tcgctgatca aaaaagcata ggcaggtgaa catgcaaaac
     7681 aggcttttac gttcaaagca agatcgtatc ctgggtggcg tatgcgcggg cttggccagg
     7741 taccttaaca ttgatactgt gctggtacgg ctgttcttca ttgtctttac cctggtgggc
     7801 ggcattggcc cgttgctgta tatcatcctg tggatcgtcg tccctgaaga ccaggccgaa
     7861 agctcgtcgg gctactcgtt caatggagag gttcttcgtg aacgcgcgga gaactttgca
     7921 gacgaatttg taaacgcggt tcagaaacct ggcaacaaag ccagttttta cgtagggatt
     7981 gggctggtgc tgctcggggt ttacattttt ctcaaaaacc ttaacctccc ctggctggca
     8041 tggttgaaca ccagtgtcat cctcgctttg ctgattatca tcgccggcgc ggcgttgctt
     8101 tacgtggcgt tgaaaagaaa aacatagtgc caattttggg cagttgttgg cttgcacagg
     8161 aacttttcgc aattcagttc gtcttattgg taaaaacaag aatgagagga aaaaatgaaa
     8221 aaatcactgt tggttatcgt tgcaagtatt atggcggtca tgtcattagc agcctgcgca
     8281 ggcagcgggc agccggctgg catgccgaat ctccgcaccc taagcgcgag tggctcaggg
     8341 gaggtttacc tggttcctga tgttgcttat atcaatgtgg gtatccgcgt tgatgctgat
     8401 gaggtttccg acgcgctttc caagaacaac gcccaggcaa acagcatctc tgaggtgtta
     8461 cagcagttgg gcgtggataa gaaagacgtt cagacctcca actttaatgt ttaccccatg
     8521 atggattatg gtatcgatgg gcaggtttca cgccgttatt acgtggtcga aaacacggtc
     8581 tatatcaccg ttcgcgacct gacgtcatta ggtaaattgc tggacgcggt agttcgctcg
     8641 ggcgccaata ccatcaacgg cattagcttt gatgtgctcg ataagaccgc agccgaagcc
     8701 aaagcccgcg acatggcgat tgccaatgcc aaagcagagg ctgaagccat tgctgcgtct
     8761 gccggggtga agctgggtaa cctgcaatcg gtgaacgtct ataccggcgg aatcgccgtg
     8821 cccatgtatg acgcgaaagg cattggcggc gcggccatgg attccgtggt tcccatctcc
     8881 gccgggcagc taaagataaa cgtagaggcg agcgtcattt acgaaattaa ataaagcgta
     8941 atggcagtac aaagagaggg tgtctccggt ggcaccctct ctttacgtct tcaaggctta
     9001 tttgtattta atcgaactga tgcgcattaa tttgctcaac ttatgggttg agataacgcg
     9061 ccttaccgtg cctgaaaggc cacgcatcac cacgctgtca gttcgggcgc catctttaaa
     9121 gtaggttacc ccacgaaggt aatctcctgt ggtgataccg gtggcggcaa aaaagacatt
     9181 atcggaagag atcaagtcgt ccatggtgag tggtttttcg aaatccacgc ccatctcttt
     9241 ggccaggtca agttcacgtt gattgcgggg gtaaagcttg ccgatgatct ctccccccat
     9301 gcagcgcaaa ccggcggctg ccagaacgcc ttcgggcgtt ccgcccacgc ccatcaatag
     9361 atcagcggta ttgtctccca tggaggtcat caatgcaccg gctacatcgc catcagggat
     9421 caacttgata cgagcgccag ccatacggat ctgcgtgatc agttcctggt gccgcgggcg
     9481 gtcaagcacc actacagtca ggtcctccac tttgacgcct ttggctttgg cgaccttgtg
     9541 caggttatcg ctcactggtg cgtcaatatc cacggcgtgc cgggctaccg ggccaacggc
     9601 gatcttgttc atgtacacaa aaggccctgg gttgaacatg gttccacgcg gagccagcgc
     9661 cacggtggag atggaattcc ataaaccgta agccagcggg cgcgtgcctt caatgggatc
     9721 aaccgcgata tcgacttccg gcccttcgcc ggtgcccagt ttttctccgt tgtaaagcat
     9781 cggcgcttca tctttttcac cttcgccgat gataatggtt ccccgcatgc tgatggagtt
     9841 cagcatcacc cgcatggcat ccaccgctgc tttatccgcg ccgttcttat ctcccctgcc
     9901 catcatcctg cccgcagcaa gcgctgccgc ttcagtaacc cgaaccaggt caaacgccag
     9961 gtttcgggtg ggtgtagtat ttggcatgat ccctccgttt aaaaaatgat gttggtgtta
    10021 tcgtgtcagt aagatgatca cgtaataccc gatggctgca gcccaaagcg aagaatccat
    10081 acggtcgaga aaccccccat gctccagcag gaaattccct gaatccttcg cgttgaactg
    10141 acgcttaagc atactttcgc caaaatcacc gaaaggcgcc aggatggaga tgaccgctcc
    10201 gagcaccagc ccataccatg gtagaatgag cggggccggt ttgtgccaca atgcagccag
    10261 tgcccatcct cccagcatgc ccaccagtat gccgcccagg taaccctccc aggttttctt
    10321 ggggctcaca cgggggagca ttttatgttt gccaagaagg cgtccgaaaa tgtatgcccc
    10381 tgtatctgcc agggatatga tggggaagac cagcaccacc caatggagcc cattttccag
    10441 ggcgcgcagt gaaatggcat aactccccag ccagcccagg taaaccgttc caccaatcgt
    10501 gatcatcaag tcgatggcgg ctgtcatccc tccgcgattc tgctgcagca cgtgaaacaa
    10561 cattgaggtc agcacaagcg cagccagcca gacgtcactg tagctgaacg cgtaaagatg
    10621 tcgcagaaca accgccagcg gcacgaaaat ccccatcacc aacagggatg gggagtattg
    10681 ccctttgtta aacaggcgcc agagctccca cgctgcaagc aagagcacca ccgcgacaaa
    10741 aacggtaaac gcccacccgc ccaggatcga aaagacgatc agcgttggaa ccatgagatt
    10801 gataacgatg agccgttttc gcagtgagag atcagttttc attgtttgaa cttgaaatgt
    10861 tcccgaacct tctgtcgcgt tgatcgtaaa tttcgatcgc tttgaacagt tcttctttag
    10921 tgaaatctgg ccataaagtg ggggttacat accattccgc gtacgcgccc tgccagatga
    10981 ggaagttgct cacccgcatc tctcccgagg tacggatgat gagatccggg tcgggtgtgc
    11041 cagcggtgaa caggtagcga tcgacaagtt cctcagtgac atcttttgag gggatcccgt
    11101 ccctgatcat tttctgaatg gcgtgcacaa tttcatccct gccgccatag ttccacgcca
    11161 ggtgtaatat cagcctggtg ttgctgcagg taacctccat cgaatgctcc acttttttgc
    11221 gcaccgactc gggcatggct tccaggcggc caatatgatg cagtcgcacg ccttccgcgc
    11281 acagctcatc cacttcttta tccaggtagg tttccaggat ggttaacaaa ccgtcgactt
    11341 cggttttcgg tctgctccag ttttcagttg agaaggcgta cagggtcagg tgctgcacgc
    11401 caatttcagc gcagcccttg ataatctggc gcaggttttc tgttccggcg cgatgacctt
    11461 ccaatcgggg taacccgtgg gctttggccc aacggccgtt cccgtccata atgatggcaa
    11521 tatgcgcggg gatctttgcc ggagttttct ttgtcattcg ggcgcctgac caggtcagat
    11581 ttccatgatc tcagattctt tgcgcttacc gatgagttca atttcatcga tcatcaggtc
    11641 tgtcagtttc tgcaggtcct gttcaccggt ttccagatca tcctttgaaa tcattttctc
    11701 tttttcaaaa tcgcgcaggt ctttgatcag gtcacggcgg acattcctga tggcaacccg
    11761 gcattcttcc agccggttgt tcaccacctt aaccagttcg cgtctgcgtt cttcggtgag
    11821 cggtggcagg ttgaggcgaa tggacttccc gtcattattg ggggtcaacc ccagttcaga
    11881 tgccagaata gaacgttcga ttgttttgat ggttgaagga tcgaaaggtt tgattaagag
    11941 cgcgcgggct tcaggcacgg atatgctggc caactgcaga agcggcgtgg gcgcgccata
    12001 ataatcgata ggtagctttt ccaccagcgc cggcgtggct ctgccagtgc gaattgccgt
    12061 caggtcttct tccagagaaa gcaaggcgct tttcatgcgg ttttcagcgt cttttgtgat
    12121 ttcctttaac atatttcctc cgccaattgc tgatttgtaa taaagcatac cttaaaagcg
    12181 ataaaaaatc cattgacatc aagcaatatc ttacagtaaa acgcatccgg cggcaggtct
    12241 tgacaaataa gaacttatgt tctataatag tgataagtct ggcaggttat tctggcccga
    12301 accttgcaat aagtcaaaca actgaaaact tatggagagt gagatgaata ctggcatgct
    12361 atggttcgat aatgacccaa aggctgattt cttaaccaaa atcaaccggg caaccgatta
    12421 ttacctgaag aagtatggcc aacgacctga tttatgtttt gttaaccctg gaatgaagtt
    12481 cgaacctcct ccaaaaaatg tagggattga agtgcaaacc gaccaaatga ttatgcctga
    12541 ccatttttgg ctgggcatta aaacctgttc agcctgatga atctggtaca acttggtgtt
    12601 ttagccgccc aactggcggc tttatttttt ctcctggttg aaaattcttt tttttaaccg
    12661 ctgccaaagt tgagaaacgt gcctgaaagg gcgctgtgaa aaccggttgg tgaaatcagt
    12721 ggctgctgat tccaggctga tgcgccgttt ctgttcttca gccaggtacc agcggtgttc
    12781 aatgatccaa aaatataggt ccatctctgt gcgggcagga aaagcgttga gaatctgatg
    12841 tttacggatg atccgcacca gtggaaggta aacctcgcgg tgccatcctt tcaccgcctc
    12901 ttcatctgta actgggcgcg ttaatttctc acccatgaac cagcggtgca cgctgatgtg
    12961 ctgcaatacc ttctcgtact gtccaggtat ggaaaaacgg aagtcttcac ctggataaaa
    13021 ttcgttaagg cgggtttgcc tgaggaactc gctatactcc tgctccagta ccagtgcgtt
    13081 cacatcggta tcacgggtga ttttgccgct ggtgtgcact tctgtcacca aagcgtctac
    13141 gtaaacctgt cccttttcac gggcgacgga tacgcggtga ttaccatctt tgacaaaata
    13201 gaaatcaccc actttataca cttcgattgg cggcaggatg atctgtttga aatgcgcgcg
    13261 gtcgatactt tcccagcgcg agcgcgtatg cgtctgcctg ggcaggaatg cacggtcaaa
    13321 atcattgtag cggctcacgg aaccgattat cttttcagtt tcgatttgcc tgaccccaat
    13381 gtagttttgt ccgcgcatcg ggatatgctt gaggacttct tcaaatggca gcagctcatt
    13441 gtttttcttc tgaatcaggt gaaaaacgga gcgccaaaaa cctttttgat aggcgtggct
    13501 aaaatcgtga cgcgattgaa gtattagacg acctgaatca atgtcttctt ctgtcatcat
    13561 gattcacctt tgaccccgcc aagagcgcgg gcgcggagcg gggatgtgca gggtcttata
    13621 gccgtaaaca ttctgaaccg tggtaccgcc gatcagcgta cgggtgatgg tttcacggcg
    13681 gtaaatatgg atgtgtccat gcagaaagta agctggttga aacactttta tcaaccagcg
    13741 aaaggcttta atccctcggt gcggcaggtc ctcttcatcg tgcacctgcc atggcggcgc
    13801 gtgagccatg aaaatatcca ggtagcgacc cagcgccagt ttattccaga agaaccttgg
    13861 caccaaactc agcaccatct gccagtactg cccctgggta tactggtatg gaccctggtt
    13921 gtatcttaaa gacccttcaa gccccgccag cagcagcccg gtgtcatcct ccacgcatcg
    13981 atgatgcacg tccactgctc cccatggata tttccgcgtt cctgccacgc cgtactcgcg
    14041 ggcgttggca tgattaccgc ggacaaaata gcaggggaca tttaacatgc tgaccatata
    14101 ttcgatatag taatagggca ggtcgccgca ggagaggata aggtccacgt cttggaacct
    14161 gtccatgatc atggggctgt aaagcaaagc attctctaca tcactgactg ccagtatctt
    14221 catctcaaca ccgccggctt atctcttctc aaccagggcg gtgacgcagg cagcagcgag
    14281 gttgcgggtg tgactgatgg aaaccgacca agagcgccag ttcatacgct cagccatttg
    14341 agcagcagca ccatacagcc ttaatacggg cttcccattt tcatccaatt gggtttcaat
    14401 atcctgccag ccaacttcgc caataccgca acccagggct ttaacggtgg cttccttgat
    14461 ggcgaaccgg gcggcgaggc gatcatcacg ccctccgcta tcttctatct cctgtgttgt
    14521 aaacaccctg gagacaaacc gggctttaat cgccggggtg atcgagcgca ggcgctcaac
    14581 ctccaccaga tcgataccac aagccacttc catcatgggt tattgttgac ctttccatcc
    14641 gcgcctgctg aaacgatgat caacttatca gggttaatga atttccgcgc cgcggcgaga
    14701 atatgttctc tggtgagcgc gcttacaagg gtggggtaac gttgcaggta gtccagaccg
    14761 agttcaaaac gctcaagatt taaaatactg ttagcgaccc cggcgttcga ttcgagggaa
    14821 agcggcatgc ggccgatata gttattctga ttgtcttcaa gctcttcctg agagacaggc
    14881 tcagttgtga accggcgcaa ttcttcaatg atgagcgcgg tggctttttc cacgttgcca
    14941 gggcttacgc ccgcagaaac ttcccacgaa ccgctttcaa tccaggcgtt cagactggcg
    15001 gaagcgtagt aagccaggcc ggctttttcg cgtactgcat caccaacacg gcccatcaaa
    15061 ccaaactgac caaggatact gttgccgagc gacgccgcga ggtactcggg atctttgcgc
    15121 ttggggccga gcgttccgat tatcagatcg gtttgactct tgcccggaat gatgactttg
    15181 cgcgtgatgg atgcttgcgg gggttttatc gccttaaagg agcatgtttg ccgtttgtga
    15241 ccggtgagcc aattgccgaa atgtttttca gccagttcaa acacccggtc tgcgctcacc
    15301 gcgcccacca ccgccagttt catcccctca ggtccatatt gctggcggtg aaagtcagcc
    15361 aggtcatccc gcgtgatatt ttggatggtc tccgtgtagc catcctcagg tctgccgtaa
    15421 ggatgatcgg gaaagagcag cgaatcaaac tccagcgccg ccgtatcgcg cgtgctttgc
    15481 ccgcggatgg ccaacccggt gagcaactgg gcgcgcaggc ggctcacctg ttcggatggg
    15541 aagacaggtg aggacaggca ttctgcgaga gtggcgaaca ataatggcag gtcttcagcc
    15601 agcgcgcgac cgctgaaact ggtggtgtga acactggcgc caaacccgag agaagcgcct
    15661 gcggattcca accggtcata caggctctgg aaatcactgc tctcggtgcc gcgcataaga
    15721 gcgagggcgg tgaagtgggc caggccgagt ttctctaacg gatccagctg gctgccagcc
    15781 gacacatatc cgcttatgac cactgacgaa ctgttgaagt tgctgcgcgt taaaagcacg
    15841 attccgttgc gcagtacctg gcgagtaata tccgctggtc cagggatgga agacgaaagg
    15901 gtgtatttgg gtatcatcgt ttcgctccat ccggacggta aatccctacc acgcgctggt
    15961 tttgattcag gtatttgcgc gctgtatcca gcaggatctc cggggtcact tctgccaacc
    16021 tcgaggtata gcgggtgaac caggagtatt gatcaaacat ctctgagtaa cccagccaga
    16081 atgcctggtt ggtaatattc tcactgccgt atgagaaaag tgcgcgagcc tgtttggctg
    16141 cgcggtcgat gtctgtttgt ttcaccgggg cctggcgcag aacgtcgagc tctttatcca
    16201 gctgctctaa aacgacttcc ggctggtgct ttgggtggac ggtgatggtg atggtgtata
    16261 gaaacggatc gtgggttgcc tgcagggagc cgttaaggct gatagcgtat tccttttcca
    16321 ccagacgccg gtacaggcga gaagtcttat tcgaaatccc gcccccaccg aacatattca
    16381 ggctggtggg cccggtgagc agactatcga taatggtgaa ggcaaaaaac tcagcttcag
    16441 ttgccgctgg cgaacgccag gcgacctgca ggtaggtggt ttcgccaggc ccggccacct
    16501 cgatgcgttt ttcttccgcc aataattgtt cccgcaagta ttccggttca ggtacggagt
    16561 gaccgtgaag cggctggtag gtctggcgga tcctttccaa tattttttca ctgtcaaagt
    16621 cacccgccaa agcaagaacc gcgttgccgg ggttgtaata cttgcgatag tgggcgtaca
    16681 ggtcatcacg ggtgatactc cgcaggtcgg cgatcgaacc gatgacctca ttcttatacg
    16741 ggtgatggtc aaaagcggcg aattgcacgg cttcatccag tagaaagaga ggttcattct
    16801 cgttaccttc acgctctgag ataaccaccg tgcgctccga gttcacttcg ttttcatcaa
    16861 ataaactgtt caccatgcgg tctgcttcaa gggtcatggc gagatcgatc ttattggccg
    16921 gggtggtttc aaaaaaggtg gtccaatcaa ggtaggtgaa agcattccaa tatccaccat
    16981 cccgtgaaat ggctttgtcc agtaccccgg cagggaattt tgctgtgcct ttgaactgca
    17041 tgtgctccac ccaatgggat atacccgtga agccgggttt ttcaaagcgc gaacccacgc
    17101 ggtaccaaac ccactggctg atgattggcg cggtgtgaat ctccttcatc aatactttga
    17161 gtccgttttc cagttccacc tgcgtgattg tttccgtcat acgacctcta accctgttgt
    17221 tgcagtaact atagcacaag cacgaaacgc ttggtaggga agttgctata ataaaagtgt
    17281 gattgatttc ctgttcatta atcaaaaata aaccataatc ataaaggagt cttcatgtcc
    17341 aaacgaatcc aggtggccat aattggcgca ggaccagccg ggttatttgc agccgagaaa
    17401 ctgacccagg cagacatcgc ggtcgctctt ttcaaccggg atatcaaacc cggcggtatg
    17461 gcggagtacg gcatctaccc ggaaaagcat caactgaagg atgggctgcg caagcagttc
    17521 gaacggatcc tttcgtatga gcaggtgcat tattttggga atacctgtgt tggggaggac
    17581 caaagcctga cgattccccg cttattggag tggggttttt ccgcggtgtt gatctgctgc
    17641 ggcgcgcagg gcactaaatg gctgggcatt ccaggtgaga acctcgaagg ggtgattcac
    17701 tccaaaaacc tggttttcca ttacaaccgg ctgccgcctt actgcactgc gcccatcaaa
    17761 attggcaaac aggctgtggt ggtcggcgcg ggcaacgtaa tggcagatgt cacgcgctac
    17821 ctcctgggtc ttccacagat ggaaaagatt cacgtttgcg tccgccgggg tccggcggag
    17881 gttaaattca ctgccaaaga gctggaatcc atcattggcg gaatggacat ggatgcgctg
    17941 gagcacgagt ttgaacgggt taaaccggtg atggtgccac ttgggcaaga ccctgaagtt
    18001 gaaaaagcgg tctatctcaa ggcgtttgga aaggcgggaa agacaggctt aaaacccttg
    18061 atcgaaatgc attttttggt ttcacccgtg aagattgagg gagatgagca gggcggggtg
    18121 aatacggttg tattggagga aaacaccctg gttttgagcg gagaaaaaac ctcggcccga
    18181 gggctgggca cacaccgatc tctcgcagcc gatacagtgg ttttcgccat tggcgacagg
    18241 gttgaagaac gcctgggcgt gccggtgtcg ggcaatgagt accgccatgt ggagaacccg
    18301 cgctacccgg tggaagggca gtcctacgag gttgaagcgc ccggcagcgg gcaggcgtgg
    18361 aaaggggtgt ttgtcgccgg ttggtcgcga ttggcgagca gcgggttggt ggggaacgcc
    18421 aagaaagatg gggtgaatgg agcggaagcg gtcgagcagt acctgcgcga agaacgccca
    18481 ggcggcggcc tcttgttgga cgaactggaa agccggcttt ccagtttacc tgacccagtt
    18541 gtgcagcaac gacacctggc gcgactgctg gctgaagaac gtaaacgcgc gggtggtgga
    18601 ccgatcgaat acgcgaagtt tacgaccaac gctgaaatgc tgcaggtaat ggggttgcag
    18661 tgagcgaaga tagctattga ggtttgcttg cgggaggaaa gctcaggata aagccgatgg
    18721 cgataatggc gcagccgatt tccatacccc tggcctgcca cacgccaaag taaggcacgc
    18781 ctggcaatgg atgtgtgccc accccaaaca catcggccag gccagaaaaa gtaacaatca
    18841 cgtaaccggt gctcaccagg cgcataccga tatctgcgct gatgctggga ggttgatggc
    18901 gccacaacgc gcgcaccgag gtgtacccgc caagacaaat gatcatgatg ccaataagca
    18961 tcaccgcgat ttggataaaa ccaaccaccg ggctgcggtc cagcccaaag atggcaggtc
    19021 tggctcctac cagaaagatg ataaaaccaa ccagcatcac cagcagggcg aaatggatgc
    19081 gcgctagcgg cagcgcgggt aaactgttgg cagttacttt gggcttgccc tcattctcaa
    19141 tggcggaatt ttctgtcatc cgtatattct aactccaaga tggcctttcc cgatattgct
    19201 gtctcccaag tataacccga aataaaagaa ggcggttacc cacgaaaatg acaacgcgcc
    19261 cagagggtcg ggcgcgttga ttcatgtcaa tattctttag gcttcagggg ctggttctgt
    19321 gggttcttct gcggtggatt gatcatcatc cttggcgatt accgctgtat cttcgtcgag
    19381 aatgccctcg agagcctgcc agtccatgtc tgcgtaagcc atcgaatcca cgcggcgcag
    19441 gctcaaaccg atccgatgca cgctgggatc gatcttgatg atgcgcaggg tgcaaatgtc
    19501 gccttccttg aggacttcct tggggtgttc aatgcgccgt tcgctgatct cggagatatg
    19561 gatcagtcct tcgatgtctt ccgctaacct ggcgaacgcg ccgaattttg tcaggcgggt
    19621 gatggtgcct tcaatcagtt gacccacctg gaattgcgca gccttctggt cccaggggtc
    19681 agattgcagc tgacggatgg acagcccgat acgcttcttc tcgtggtcga tattgatcac
    19741 ttttactttt acttcctggc cgactttgag aatttcgctg ggatgttgaa ttcgctccca
    19801 ggagatttct gacatatgga ccagcccgtc ggcgccgttc acgttgacaa agacgccaaa
    19861 gtccgccagg ctggtgatac gacccaggta aacttcgcct tccttgagct cttccagcac
    19921 gcgctccttg accgattcgc gggtttcagt gctggcctgg cgttcagaaa ggataaggcg
    19981 gcggcgttca cggtctactt cgatgacgca aacatcaatt tcctgcccaa tcatttttcc
    20041 ccaacgctgt tcaggggtat cgccggtgac gccggcgcgg cgcgtcaggc tgatctgaga
    20101 ggcaggcaca aagccgcgca gcccgccgat gggcacgatc agcccgcctt tgttgtaacc
    20161 gaggatggtg ctgtgagtgg cctgtttgct cgcttgcagg gcttcaactt cttgccagct
    20221 ttcttcttca cgggcgcgca tgtaggagag aaccaggttg ccgttttggt cttcaggatt
    20281 gacaacataa acagaaatct catcgccaac ttttagattt gccagttcgt cgcttgggat
    20341 cagttcgtat tccttgccgg tgattatccc ttctgatttt gtccccacac tcaccagtat
    20401 ctgccctgga gtgatgcttg cgattatccc tttgcggatt tcaccctggg tgggaaaatc
    20461 aatgcctaag ccttctttct caagcagaga agccatgttg ttgtcgttct ggttattgtc
    20521 taaatttgct cctgatccat tgtgcgtgac attctgatcc atatgatttt tgaactccgg
    20581 cgtttaagat tagccataat gagttcaatc gaatgattga taacccaagg ctacaataat
    20641 acatcattat acatcatttg aatcgatatt ttttatcgat tcaaatggtc tttcagccat
    20701 gtctttgtaa atcctcttaa tgacgttttc gtttcttttt cggcgaggac tgcgctggcg
    20761 gtatttgtgt ttgcgctgct gggctggcgc cttcaggggc tgatctttca gcaggctgtc
    20821 cgccggggcg gcgcggttga aacaggaaca tacggctgat cacaccgccg cgaatgtcgc
    20881 tgagcaggtt tttgaacagt tcagaagcct gacccttgta ctgtaccagc ggatcacgct
    20941 gcgcgtaggc ttccaatccc acggagactc tcaacgcgtc aacccgggtc agataatcaa
    21001 cccacaattc agagatcaca ctcaataaga tatgcctgta gatttgattt tgcacacgcc
    21061 gaccgagcgt tttttcgacc gcggtgcgcg cttctgccga gaggtcatct ggctgggcgg
    21121 tttgtaaact gagaaaagca gcatctccaa cttcnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnncgcgct tgacctggtt attgaccttt tcttttacct cgtcgtctcc
    21301 ctctggcagc gccctcatct cttctgttgt caggcgcagc tgggtgcggg tgagattgac
    21361 cagctcttcc agtatctctt gcggtcggcg gggcgcttct tcttctccgt ggctgctacc
    21421 gagcccatcg aaaatggcat ccacgctttc cagccgctcg gcggtgagct gatcgatggt
    21481 ttcttccgtt ttttgaagca acagactggt ggcgttcagg atatgctcgt tttcagcctg
    21541 cagcgctttt tcagtgattt ctatgatctg gtctttaatg ttgctgtcat cggtttgacc
    21601 gttgatacgc cgttgcagtt catcaacaat caggcgaatg gtgtaggaag gcgtgcggat
    21661 accttctgct tcaaaaacca tggaaggctg tacttcttcc aggtaactca ataacttcca
    21721 tggaccttct tcatctttca gcgcggctgg gatgcgttcg ttgatttctg tgcgcagaat
    21781 ttcaagcacg tcttcatgca ggctctcttt ttcgaaggcg cggttgcgtt cggcgtaaat
    21841 gcgcttgcgc tgcgaattga gcacatcatc gtattccaac aggtgctttc gcacatcaaa
    21901 gttgttgcct tccacacgct cctgcgcctg ttccaccatg cggccgagca aaccgctctc
    21961 aatgggcagg cttggatcga ccttcatgcg ggtcatcaac ccttccattt gcgcgccacc
    22021 gaacaggcgc atcaactcat cctccaggga taggtaaaaa cgagaagatc ccgggtctcc
    22081 ctggcgggcg gcgcggccgc gcagctgatt atcgatgcgc cgcgcctcgt gcctttctga
    22141 gccgatgacg tgcaaaccac ccagggcgcg cacgcgcttc atatcctcaa ggtaacgaat
    22201 aaaggtattt accgactcct cgtagatgcc gtaatcctcg agcgtaagtt tttccagttc
    22261 accgcggcgg gtatcgttgt tcaaatcgta gggatctgcc acaccggcct tctccagcac
    22321 gcggatcacg tcggccagca cttcctcgtt cagttcaccg ccgagcttga tatccacccc
    22381 acggcctgcc atattggtgg cgatggtcac cgagccaaaa gcgccggctt tggcgatgat
    22441 ctgagattct tcatcatgtt tgcgcgcgtt caatacctgg tgcggtacgc cgccttttaa
    22501 tacttcaagc acgcgctctt tgtggggcac ctccaggtcc attaccttga gcaggcgttc
    22561 caacaaatca gggtcttctg ggttgatcga agaaagcccc gcctgtcggg cgagcgggcg
    22621 catttccgcc acattgagct catgcaaggg gcggttgaga aattgcagtt cggtgatctc
    22681 gatttcggct gatttattga acttttccag ccaggcatcg cggatgacca gggtttgtgc
    22741 caggcggcgc accgattcag cgctgagccg ctgtgaaagc cgttcagaat gttccacgga
    22801 ggtcgtgccc accagttgcg gccgaccaat ggcgtaataa tggatgattt ctgcgacaat
    22861 cgctcgcagt ttaccctctt cggtgcggta aaccaggtca ggaaagtcct gacgacgcca
    22921 aaaaaccgct tcgtgatgag ggtcatcctt cttgctgtaa tactgcaggg ggtaattttg
    22981 ccggtcgcgg gtctccacca cggtcagcgg gctgtcttgc tgggcggcca cgtattccag
    23041 gttcatggga atgggcagca cttccagctt atagatcttc gaaaattcct cggcttcggt
    23101 cagggcggta ccggtcatgc cggccagttt ttcgtacatg cggaagtagt tctgaatggt
    23161 gatggtggcg taggtcacat tttcaggttc aaccttgacg ccttctttcg cctccacagc
    23221 ctggtgcaac ccttcagacc agcgcctgcc gggcataagc ctgccggtga attcgtccac
    23281 gatgataacc ttgccgccct ggacgatata atccttgttg cgtctgaaca ggtgctgggc
    23341 gcgcagcgcc tgctccaggt agcccagtaa ccgcgcctgc tcgggggcga tatcttcagg
    23401 tcggtcaggg ttgcgcagtg gctggttgag tatctcttcc acgtgggcgt cgccaatttc
    23461 agtcagtgaa acctggtggt ctttctcgtt aacctcgata tcttcggggt tgagggcgcg
    23521 caccacctgc gccatacgca catagtcttc cgtttcatct gacgccggtc ccgaaatgat
    23581 caacggtgtg cgcgcttcat cgatcaggat gttgtcaact tcatccacaa tggcataata
    23641 atgccctcgc tgcacacgtt cttccaggct catggtcagg ttgtcgcgca ggtaatcaaa
    23701 gccgaattcg ctgttggtgc cgtagacgat atccgctttg taggcttcac cccggtctac
    23761 cagcgcgagc atgtgcaggt cttcgcgcgc gttgggtttt tccaggtcca ccaaaaaggc
    23821 tttgcgacca ttttctgtgc gggcagccat ctgcagcaca cctactttca accccagcag
    23881 gttgtataca ggagccatcc agcgcgcgtc acggcgtgcg aggtaatcgt tcactgttac
    23941 caggtgaacg ctgcccccgg tgagcgcgtt aaggtataaa ggcagcgtgg cgaccagggt
    24001 tttcccctca ccggtgcgca tttccgcaat cttgccgtga tgcagggtca taccgcctat
    24061 catttgaata tcgtaatggc gcagcccgag cgtgcgcttg ctggcttcgc ggacagcagc
    24121 aaaggcttct ggcagcaggt catccagcga ctcgccttct gccaaccgtt gcttgaattc
    24181 gcttgtttta tgattcaggg catcgctgct caggccctcg aagaccgttt cgagctggtt
    24241 gatttcgtcg acgatctgcg agagatgttc aatttcgcgc ttgtgagggt cacccccaaa
    24301 gacacgggca attttttgta agaccatgtt cacctcttcc agtcaggagg tgattatagc
    24361 atagggtatt ttcgtttcag ctcagtggaa cgcctttcag tatatgctgc aggatgtcag
    24421 taaagctggc gccaagggcc aaccctatag cgacgaagat acaaccgaag acaataccga
    24481 tgagcaaatt cgccacgaag tacaagcccc acactgaaaa ggcgcgggtg agaaaattgc
    24541 tggaaaggat acccagctcg ggcatggact gacggctggc tggcggtttc tccagctctt
    24601 tttcaattag ggcaacacga gtaacgaggt tggtaatgtc ctggtctgtg tacactgttc
    24661 ctcccgataa tcctgtaatc tggtttttct atccgtgctg agcgtcacac catccgattt
    24721 ctatcaaata tgaatggctt tcccctcgac catttcggcg gcttccatca acacttcgct
    24781 taacgtgggg tgggcatgga cgttacgggc gatatcgcct gctgtcaatc cgttcagctg
    24841 agccagggtt aactctggca gcaattcaga gacctccggg ccgatgagat gggcgccaag
    24901 aagatgacca ctgtgttcat ccaccaccag cttggcaaac ccgccgtaat caccgagacc
    24961 cagggcttta ccatttggct ggaaggggaa tttccctatc ttcactgttt taccgagcgc
    25021 gcgcgcctgg gtttcggtat agccaaaaga tgccacctgc gggtgagaat aggttgcccg
    25081 cgggatcatg gtgtaatcca ggggtgtaac cgccaacccg gccagcgctt cagcacagcg
    25141 gatgccctgg gcagaagcca cgtgggccag cagcagttta ccggtgacat cgcccaccgc
    25201 ccagatattg ggcacgctgg tggtgagttg atcatttacc ctgatgtgct tgcgctctgt
    25261 caattccaca cctatttctt ctaacccaag gttctggctg ttgggtttaa acccgatcgc
    25321 caccagtgct tgctcggctt cgatgacgct ttcgccattt tgaccgctgg tgctcacccg
    25381 cacgccaccg ggagttgttt caacagccaa tacccgggtt tcggtcaggc aggtgatacc
    25441 gcgttttgaa aaggttttag ccagttccgc gctgatctct tcgtcttcca gcggcgcaat
    25501 ccgcggcagc atctccacca acgtgacctt gctgccgtag gcgttccaga tggtggcgaa
    25561 ttccaggccg atagcgcctg ctccgatgat caccacggat tgcggcagtt tctcctgcaa
    25621 gatggcttct tgataggtca gtaccttaac accgtcaatg ttcacgcctg ggagagaaaa
    25681 aggtgtgctg cccgtggcaa tgataaaatc cctggctttc aattcctgcg cggtgccgtc
    25741 cttcccggta agggtgacgc tttcacgaga gctaaacctg gcttctccca tgtacacggt
    25801 gatattgttc ttcttcatta aataagccac gccgcgtgtg agcctgtctg aaacctggcg
    25861 gctgcgttta acggcggagg tgaaatccag ctgcaagttc tccactgaaa aaccaaattc
    25921 tcgtccgcgt tcgcgcaaag taagcgctac atccgcgttc ttgagcaagg atttcgatgg
    25981 aatgcagccc acgttcaggc acacaccgcc cagccagcgc ctgtcaatga tggcggtctt
    26041 ttttcccaat tgcgcggacc gtatggcggc ggtgtacccc ccgggaccgg cgccaatcac
    26101 aataacgtca tactcttcca tggtgacact cctcgttggt tgatgagttg attatacttt
    26161 ttcccgcagc accctgaccc aggaagataa cccggtcagc catcttcgcg ggatatggga
    26221 cacggctggt gcacaagcag ggcgcaaaaa aggacaacag tcctatgatt tcctgaaaga
    26281 ggacttttta ctggtgaaga cgttgtactt gatgatgtac cataatgcca tcaagccgtc
    26341 tgtggcgttg atctttttgc cctcttcgta agtccgaccg taataagaga tgggcacttc
    26401 atacacacgg cacttcaact tggctacctt cgccaccact tccacttcga aaccgaaacc
    26461 ggtcgaagtg atgaccatat tgcggatgat gtctccgcgg aaggctttat agcaggtttc
    26521 tacatccgtg aggttaaggt cggtaaatag attagaaagg aatgtgatga gcctgttggc
    26581 catgtagtga cggaagtaca acgagcgcga ggcgcggcgc accaggaaac gcgagcccag
    26641 cacaacatcc gccttgtctt cttggatggg ggcgaccaca tcattaattt ctgccgggtc
    26701 gtattccaga tcagcgtctt gcacgatgac gatttcaccg ttggatcgtt tgaacccttc
    26761 tttcagcgct tctgtctttc ccatgttgcg gggctgctgg taataatgca ccaggggatt
    26821 ccgggcttcg taatcgcggc tgatctgacc ggtgttgtct ttcgagccat catcaatgat
    26881 gatcacttct ccaaggttgc gcactttttc cagcaccagg tccaacacct gcgagatggt
    26941 tttttcttcg ttatannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccaat
    27061 ggcgcatacc gcggccgtgc cctccaccct ttccacaggc ggcaggtcca gcgtgtaacc
    27121 gatgaaagat ttacgagaag cccccagcag cagcggatag cccagggctt tgatctcacc
    27181 caggcggttc accagctcca ggttttgctc cacggttttc ccaaatccga tcccggggtc
    27241 gaggatgatt cgccggtcat cgactcctgc cgcgcgcgcc agcgtcacgc tctccagcag
    27301 ttcggcgcag atatcttgca gaagattggc atattcgatg ccgatatagc gcccgccaag
    27361 ccgctgttgc aacttggcgt tggctggctt gctgcggttg tgcatgagaa tgatgggagc
    27421 ctgataacgg gcagcggttg cagcaatcag cgggtcagcg cgcagccccc atacatcgtt
    27481 gatccaatct gcgccttttt ccaatgctgc cgctgccaca ggcgctttat aggtatcaat
    27541 ggagagcagc acttcaccgc ctgaactcct gatagcctcg atcaccggga tgacacgagc
    27601 cagctcttct gcctcattca cagcctccgc gccggggcgc gtgctttcgc cgccgatgtc
    27661 cagaatatcc gcgccggctt caatgaagcg ctgcgcctgc tctaacgcgg cgcgtacggt
    27721 gtcgggctgt gagagcaaac cgtcacctga aaaactatcg ggggtgacgt tgagaatgcc
    27781 catgacatac gttttcttgc cgaattctgg aagggtttgt tggcgcattt ttccctcgtt
    27841 ctagggtgct ggcgtcacga agatgggtaa agcgagatgc cagttgtatc caggctggac
    27901 agcacctggt tgacggattt accgctgccg ggaatgatca gttcagggga gagctccgcc
    27961 agtgggagca tcacgaacgc ccgttcaagc atacgcggat gcggcacctg caggcgggca
    28021 gagcggatca cccgttctcc atagagcaaa atatccagat cgatcacgcg cggcccaaag
    28081 cgcagggtgg cttttctacc taggtgtttt tcgatgttct tcaggctgtc gagcagtttc
    28141 aacggcggga gggtggtgtc tccgcgcagt acctgattca agaaaagtgg ttgttcgagc
    28201 acaccccagg gaggtgtctc gtacaggcgg gagcagcgca tttcggtgat aaattcggac
    28261 agtaactggc gggcgcggta caaaaactca gcgcggtcgc ctaaatttga gcctagcgcg
    28321 atatagacgg tctctttttt tgccattgcg cgcctgtgtt cctttcaacc gccgcgcagc
    28381 gcgttaatca ggtctgccag cagcccatac gcggtggttt ccggtccggg gttgctttcc
    28441 agaatgccca gcccgggcag cgtatccatt tggaactgnn nnnnnnnnnn nnnnnnnnnn
    28501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28561 nnnnnnnnnn nnnnnnnngc ccgtacgcga cagcttcctc aaatgtccgc ccgctttcca
    28621 tcatttccag cagcaggttt gtgcaggagt tgagaatacc ttgaaaacct tgcagttcga
    28681 ccgcgggcag cgctccacgg aagagtgaaa agataggccc gccatccatg acggcggatt
    28741 caaacaggaa gcggcgttgt ttttgcgctg ccaggcgggt cagctctgtg taagcgtgca
    28801 cgaccgggcc tttgttggcg gtgatggcgt gcatgccgcg ctccagggcg gcgcgcaggt
    28861 agctggcggc aggctgaccg ctttggtggt tgaccggcga gttttcaaac agcacatccg
    28921 ccgggacgct ggcgatgaaa tctggcaccg atgcgggggc tggaactgca gacagcgcgc
    28981 tgatagcttg ccctttttgc gcgcagcgca gcgcctgcgg ggtgtcaatc ccttcggggt
    29041 caatggccac cccatggtgc ccggtggcga tacctgtcac ctgggtcgaa aaaccaaatt
    29101 cagcccgcaa gcgctcttgt ttgctttcca gcaatgccat cagcgcctgg ccgacgtgac
    29161 caaaccccac aagcgccacc cggatcactt ccattcgcgg ctcctgtgca ttcgccgggt
    29221 cttcatgcgc gctcgcatac ttcgccatct cgccagaaga ccctgccgac agggtgtttt
    29281 tgctgaagca gaagatacag ggcgtcttga aactggatgg ggtttcctga ggtgataaag
    29341 cgtggaacgc tcacttcttg accttctacc agcaggttgt gcgcttccag cattcggcgc
    29401 agctgcctgg cgatggctgg cgctggatcg atcacgcgaa cctcctcccc aacgatttgt
    29461 tgaataaggg ggatcacaaa aggatagtgg gtgcagccca tcacaatcgt atccactcct
    29521 ttttccatca ttggggttag agcgctctcc agaatgcggc gcgtagctgg accattgaga
    29581 tccccttttt ccacctgggc taccagcccc gggcaagggt cttcaaagag agtgattccc
    29641 tgaccgaagc gttcaaccac cgaggcatac atggaagtgt gaaatgtggc ttctgtagcc
    29701 agcacgccga ccttgccgct gcgggattgt tctacagctg gtttgacagc aggttccatg
    29761 cccacaaatg gaatggcggg gaactcctgc cgcagcgcgt gcagcgcggc gacagaagcg
    29821 gtattacagg cgaccacaat aagctccacg ccctgcctga gcaggtattg cgtgatgccg
    29881 cgggcaaacg cctgcacttc tgtgagctgg cggccgccgt agggcacatg tgcctggtcc
    29941 gcgaaataga tcacaggctg agccggcagc gcggtgagaa tctcacgcag aacgcttagc
    30001 ccgcccactc ccgaatcaaa aacccctacc agatgctgcg atttattcaa tcagcgaaag
    30061 tcctttttat tgtgaagccg cgtgaaggaa tgccctgaaa attgcgcggt gttcttccat
    30121 cgcctgcagc gattcaggat gccattgaac ccccaggcag aaacgctgcc ctgggtgctc
    30181 gattgcttct attaaaccat cacttgcctg cgctgtcacc tgcagcccta tgccagtttc
    30241 cctcaccgcc tggtgatgcc ggctgttcac cctgagctca ctgcaagcag tcgcccggcc
    30301 cagctgtgaa gcaggctcaa tggttaccgg gtgaatgagt ttgttgggcg gcacgtcatt
    30361 gggctggtta tgcgtgatgg atgtgtcata ctgtctggga atatccgtga ataatgttcc
    30421 gccaagcgcg gtattcaaca tttgcagccc gcggcaaatt cccagcaatg gccaatccgt
    30481 ctttactgcc agttcgacca gcgctttttc caacgcgtct cgttctttta caatgctgtt
    30541 cgcgtaaggg tcgggcctcg cctggtaaag catcgggtca atatctcctc ctccgcttat
    30601 taaaatgccc tgcagcaccc cgcgcagcac ctgcaaccga tccagcgggt attcattggg
    30661 aatcaatacg gggcagccgc cggcaaggtg tacggcgttg gtgtattcgc ttagcagcat
    30721 gttggctttg cgtttatggc tccgagagat ctggctgacg gtaatgccaa tgagaggggg
    30781 tgccatgggc gctccttggg tcatttgtat agaaaagggc gcaaccacgg cgttggttgc
    30841 acccggaagt atgcgaacga agatggatca gttaaatttt tgcttaaggc gcgcgctctt
    30901 gcctgaacga tcgcgcaaga agtacagccg cgagcggcgc actttgctgt ggcgctccac
    30961 aaccactttc tcaatgcggg gcgagcgcag caggaaggtt ctttcaacac cgataccgtt
    31021 gctggcaaca cggcgtactg taaaggttga atcattaccg ctgttgtgct ggtaaagaac
    31081 aactcctttg aactcctgga tacgctcacg attgccttct ttgattttca catgtacact
    31141 gaccatgtcc ccggttctga ggctgggaat atttggattg acgggggcat cgtatgcttt
    31201 taagatatca ctcattttac acctgctttt tttgatcatt cgcc
//
LOCUS       DAOSPM010000008        29991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1551_length_29991_cov_14.512761, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000008 DAOSPM010000000
VERSION     DAOSPM010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 29991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29991
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1551_length_29991_cov_14.512761"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLW25_01650"
     CDS             <1..732
                     /locus_tag="PLW25_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOS53224.1"
                     /translation="GGVSTPLIERNTTVPTRKSQVFSTASDSQTQVEIHVLQGERPMA
                     ADNKSLGKFNLDGIPPAPRGVPQIEVTFDIDANGILNVTALDKATNRSQHITITASSG
                     LTESEIQQMKRDAEANAEADRKRKELVEVRNNADNTIYAAEKLLRENSEKIPADTKKE
                     VEEAIASLRGLMNNEDAGAIRTATEALGQSIQKIGASMYQQQPEAGGQGAPGEQQGQP
                     GTDSNGGNPPDGDAGDVVDGEFKSV"
     gene            831..1946
                     /gene="dnaJ"
                     /locus_tag="PLW25_01655"
     CDS             831..1946
                     /gene="dnaJ"
                     /locus_tag="PLW25_01655"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOS53225.1"
                     /translation="MTKRDYYEVLGVNRNASAEELKSAFRTLARKYHPDMNKEADSEE
                     KFKEINEAYAVLSDPDKRRAYDQFGFAGVNGAGGVPDWTTMDFADIFGEFFGFATGAS
                     RARQNAPRRGQDLAYTVTLTFEEAIFGVEKEVEITRDEVCHTCGGKGAEPGTNPAKCS
                     NCAGRGEVRQVRQTFLGSMVQVTTCPVCAGRGEIIGTPCHTCSGRGLERKTLSKKVAI
                     PGGVDNGTQIRLAGEGQPGINGGPTGNLYIEIKVKSHKFFRRKENDILLDLNINIAQA
                     ALGADVEIPTVDGPTKLVIPAGTQPGKVFTLRGKGSPILRSNARGDQKVIVNVEIPTR
                     LTDEQRQHLQALAASMGTEVKPQEKGFLDALKEVFGG"
     gene            1939..>2840
                     /gene="prmA"
                     /locus_tag="PLW25_01660"
     CDS             1939..>2840
                     /gene="prmA"
                     /locus_tag="PLW25_01660"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOS53226.1"
                     /translation="MAEGKWLQVSLTVDGELAEAVAEVLNRFTSGGVVVESDVIYRNA
                     EDEGTPYGAVRVFGYLLIDENIEVNRHKLEEALWHLRAIRELPQPEYREIADENWMEA
                     WKAHYHPIPIGKKLLILPAWIKQKDQSRLAVKIDPSMAFGTGTHPSTQLCLELLEDYT
                     APGKPVIDIGCGSGILSIAALKLGASKAIGVDIDAPAIKATHENSRANGVEEKVETGL
                     GSVEEVKRGDFSARQAPLVLANILAPVLIHLFEVGMAQLVSPGGVILLAGILSEQGAS
                     VRAAGEARGLTFVEERCCGDWSALV"
     assembly_gap    2841..2940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2941..3170)
                     /locus_tag="PLW25_01665"
     CDS             complement(<2941..3170)
                     /locus_tag="PLW25_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53227.1"
                     /translation="MIHPLVAQLFFTRSEFERGLEGVSAEDAVKHLGPMNCISWIIGH
                     MTWHEQKIWHDLLHGKTHYPDFVNRFAHGAPK"
     gene            complement(3293..3697)
                     /locus_tag="PLW25_01670"
     CDS             complement(3293..3697)
                     /locus_tag="PLW25_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOS53228.1"
                     /translation="MSSASVKWIGGKQFVGMDSTRHTVVLSTPDEGVGMKPSELMLVA
                     LASCSAVDVVEILNKKRAGLTSLEISAEAEQEQDPPWTFRKIHLTYRMKGSALTEKDA
                     AQAIELSEGKYCSVAATLKGKAEITTSLIILE"
     gene            complement(3714..3986)
                     /locus_tag="PLW25_01675"
     CDS             complement(3714..3986)
                     /locus_tag="PLW25_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOS53229.1"
                     /translation="MKVTSENGKKQVINRLNRLEGQIRGINRMLAQERDCSDILQQFV
                     AARSALQAALDVYTGQIVNDCLLADDLSLEQHRKLAVEMLAVIRKT"
     gene            4397..5122
                     /gene="lexA"
                     /locus_tag="PLW25_01680"
     CDS             4397..5122
                     /gene="lexA"
                     /locus_tag="PLW25_01680"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOS53230.1"
                     /translation="MARRQTGLSERHRKIMEFLTRFQDSHGYSPSIRQIGDSINVKST
                     SLVDYYLNQLQNLGYIEREDRISRSIRVLQPIQPGVSLPARVGEAFRGAASALASMVS
                     IPIAGRIVASEPIPMPVSDLNYYDAESSVDIARSLLPAKDVNDLFALEVSGDSMIDAM
                     INDGDIVVMKKASSANNGEMVAIWLDDNNETTLKYFYKEADRIRLQPANPNMEPIFVK
                     NPKSLRIMGKVVMVIRQVHSVAG"
     gene            complement(5388..5978)
                     /locus_tag="PLW25_01685"
     CDS             complement(5388..5978)
                     /locus_tag="PLW25_01685"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOS53231.1"
                     /translation="MSRKIIPLLVILALLTLSCSRSLAGKTGSTPAVVPIGTDFLKAT
                     VEAMRPPTITPTPTHDITLGTVTPIPPTNTPIPTSTPVQDPNCYLGLEASDITVEDGS
                     EIKAGEHFTKTWKLLNTGSCYWTGDFQLVFISGEPMSANSPQLLGSTVYSKKEIEMSV
                     DMVAPNTTGNYIGYWQLQMPDGYPVGWLWVEILVVP"
     gene            complement(6114..6962)
                     /locus_tag="PLW25_01690"
     CDS             complement(6114..6962)
                     /locus_tag="PLW25_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOS53232.1"
                     /translation="MIGIITDSTCDIPDALIEQYGITIIPHYIIWGDEQFRDRVDMTP
                     LAFYKRLEEDPVYPSSSQAGIPDFQKTYEEVAAKGATEIITLTVSSAMSGAYHMACEA
                     ARLVKIPVSVVDGKGPTMSLGWQVLAAARARDAGMEVKKILTMLDDLKKKLVQYVAMD
                     SLEYLQKSGRIGNAVKWVGSLLKIQPLVSINHHSGLVEPAAMARTHKQLVEMLYQKFF
                     AQLRSHNRLHIAVLHGNSLAEAEALAERIRPEFNPVELILNITGPVLGISTGPGALAL
                     CGYSEE"
     gene            complement(7053..7214)
                     /locus_tag="PLW25_01695"
     CDS             complement(7053..7214)
                     /locus_tag="PLW25_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53233.1"
                     /translation="MMPDTSAYMVAGFLVIIGGVAIYALWLLTRFNDCAKRINTLKEL
                     EKGKRPYGE"
     gene            complement(7211..7924)
                     /gene="ccsA"
                     /locus_tag="PLW25_01700"
     CDS             complement(7211..7924)
                     /gene="ccsA"
                     /locus_tag="PLW25_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOS53234.1"
                     /translation="MKPEKTPRALIFLDFLALLALATAFALVIFYAPFELTMGSVQKV
                     FYFHVAAGWAGMLGFFVATIAAVACLKTGSLKWDVAGLAGVEIGMVLMALCIVSGSIW
                     ARPVWNTWWTWDPRLTTALIMELIYAAYLLLRSGLDDARRRAKLAAVYAIVGFISVPL
                     TFFSIRVFRTIHPVMIGSADSSANELFAMNPSMQTAFFSSLAAFSILFIALYWHRYRL
                     GNLQAEINERRAALQEEGE"
     gene            complement(7937..8611)
                     /locus_tag="PLW25_01705"
     CDS             complement(7937..8611)
                     /locus_tag="PLW25_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOS53235.1"
                     /translation="MKPFIGAILAIFWKDLLAERRTREILGTMLVFALTVILVFVFAF
                     DLSVEIRRKAAAGVIWVTLCFAGTISLNRSLTLERDREAINALLLAPVDRTAIFFGKA
                     LVNWVYLLITAAIIVPVYALLNNLSLFSWGGLGVILLGTLGYILTGTLLSTLTLQLRA
                     RDMLLPVLLFPVIIPLLLACVRASTILLQGGNPAELDTWLVMLVGYDLLFAAVGLLVF
                     DRIIEE"
     gene            complement(8608..9339)
                     /gene="ccmA"
                     /locus_tag="PLW25_01710"
     CDS             complement(8608..9339)
                     /gene="ccmA"
                     /locus_tag="PLW25_01710"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HOS53236.1"
                     /translation="MLNAVNLTRSFGNRQVLRGLSLTVEPAEIVSLVGINGAGKTTLL
                     RVLANLLRPEQGMVRFGSYTPLNAPAEYRRHIGVVMHTSMLYDNLSARENLRFFSRLY
                     QIADSDKRIRQVLEMVALNSRADELVRTFSRGMQQRLAIARALLHDPDLLLLDEPYTG
                     LDQRSSELCEMLIRQAAADGKMVLLTTHDFNQAVAISTRVDLLHDGKISWSSPSAGLS
                     GKSLRATFNELTVFSGSHKGHGVNA"
     gene            complement(9356..9655)
                     /locus_tag="PLW25_01715"
     CDS             complement(9356..9655)
                     /locus_tag="PLW25_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53237.1"
                     /translation="MMLIPIVITVILLALVFFYITRPLFTHQPLGGSTYAPDVKALKQ
                     AAYQAILERIRELDFDFGLGKMSQQEHEEQRTLLLQEAAVALRELKMVDSPPGSA"
     gene            complement(9671..10204)
                     /locus_tag="PLW25_01720"
     CDS             complement(9671..10204)
                     /locus_tag="PLW25_01720"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOS53238.1"
                     /translation="MNKAKKRFPYWMVIFGLLLVGLLITLGLWLKQSRQTGFEVGAEI
                     PDFSITTFSGEHYQKSRMDGRVILLNFWSSWCASCEEEGAILEEVWREVKDSDDIIFL
                     GVNYVDTENDAKAFLEKYGITFPNGPDMGSRISRLFKVQAVPETYLVDKDGRLAAVQI
                     GPFQSADEIRAMLELAK"
     gene            complement(10234..10713)
                     /locus_tag="PLW25_01725"
     CDS             complement(10234..10713)
                     /locus_tag="PLW25_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HOS53239.1"
                     /translation="MKRVLLVIWLFFLLIVQTPGYAQTEPPVSDDEVNRLASELYCPV
                     CENVPLEVCPTKACAQWRELIREKLALGWNDEEIKAYFATQYGDQVLAVPPRRGFNWL
                     IYLVPPLTLVAGILIAIRVIRKPKTIPAAQEVSNEGPCRMAAEDFISQIENDLEGVE"
     gene            complement(10710..12749)
                     /locus_tag="PLW25_01730"
     CDS             complement(10710..12749)
                     /locus_tag="PLW25_01730"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HOS53240.1"
                     /translation="MIGGIGYGGLVIALVTTLTAVITSLCGGRSRSDALIETARRAAL
                     ITFPVLSLSILALLYLLASGRFEYSSVYQTTDLSMPLYLKLAALWGGQEGSLLFWCWL
                     LAGFTFPVVARRWKNEADLLPWVIAATSFTLLFFIILVLFFENPFLRYFITLDGIIKR
                     AFFPPAMSILAKPTDGTGLNPLLRHFGMIIHPPALYLGFTGFVIPFAYAFAALVSGRT
                     DDHWLKSTRKWALLAWLFLSLGLVLGSRWAYDVLGWGGYWGWDPVEISALMPWLTGTA
                     YLHSAITQEKRRLFQHWNIALIVLTFCLVILGTFLTRSGMLSSVHAFSQSAIGPAFFV
                     FIILMFSISFGLLLYRWSALRSENQIHSVFSRESAFLFNNLLFTALLLICLTGVLFPI
                     FSELLTGQKVTVGPPFYESATAPLFGLLLLLMAIVPLTMWGVSTSKNLERHLWKPALA
                     ALAVPLIVFLAGVRNWFALLAVWLVTLAIFTTVLDTYRTVTLRASNRQEPVRQAFGTL
                     VRRFSRRYGGYLIHIGVALMALGIIGIEFFQTTTQGMLAAGETLSLSGYTFTYEKLEV
                     TDNADGRNSARASVMIKRDNRELKRVFPGSDTYYVSQQSVTLPGVRSTLVDDLYVILV
                     DWQPISSEGATFKIYRNPLINWLWIGSGVLVFGTLVALWPTTRRKNIPRTHQEEK"
     gene            complement(12746..13261)
                     /locus_tag="PLW25_01735"
     CDS             complement(12746..13261)
                     /locus_tag="PLW25_01735"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOS53241.1"
                     /translation="MNKSPRAVEPHPRFKIHKFAIGGMLLLAAVVLLVITSLKGNAQY
                     YLTVEELASGKGVNNANVRVSGVVLGDTIQYDPQTLDLTFTIAHIPGDNREIEAGGGL
                     ANVLHNASLDPQAARLQVRYNGVMPDLLKNEAQAILTGAMGDDGVFNAEELLLKCPSR
                     YEAEVPAQAVP"
     gene            complement(13328..13714)
                     /locus_tag="PLW25_01740"
     CDS             complement(13328..13714)
                     /locus_tag="PLW25_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53242.1"
                     /translation="MKFFNLGLPEIIFIVVLALILLGPGNMVKSAREVGAFIRRITRS
                     PYWQEVWATRREINEIPRMLAREANLDETIRDLDRQSRGVQSSLTRSVADLIKEVEEP
                     RKDNQADINRESPDLHEAPEPPAERE"
     gene            complement(13763..13939)
                     /gene="tatA"
                     /locus_tag="PLW25_01745"
     CDS             complement(13763..13939)
                     /gene="tatA"
                     /locus_tag="PLW25_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOS53243.1"
                     /translation="MFSKLGVPELIIILVIIALLFGVGKISKIGGEIGGAIRAFRKGL
                     KGEEKEEKDDSTQE"
     gene            complement(13995..14741)
                     /gene="tatC"
                     /locus_tag="PLW25_01750"
     CDS             complement(13995..14741)
                     /gene="tatC"
                     /locus_tag="PLW25_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOS53244.1"
                     /translation="MEQETSGMSILSHVNELRKRLLISVIALAVATVIGFVFSRQLAE
                     VLAGPIGGLSAMKSIDVTENVTAFMKISLLAGVVLALPIVVYEIFAFVLPGLKPNERK
                     WVWFMIPLATLFFAAGVVFAYFVLLPTALPFLLNFMGIATAPRPSTYFGFVLNLLFWV
                     GLAFELPLIVFLLARIGLVTAKQLARQWRIAIVIIAILAAFITPTPDPVNMGLMMIPL
                     CALYLISILFAVAAGHNRKKSQQADGAGAG"
     gene            complement(14995..15816)
                     /locus_tag="PLW25_01755"
     CDS             complement(14995..15816)
                     /locus_tag="PLW25_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HOS53245.1"
                     /translation="MKTHTSYEKMIQRERRKREQNLLQSPLNWFSLVGLYPLQTGENR
                     LGHGSGYTIHIPELAQGAEALITLTGDEIWVEVGSHFTINGAPAAERQLRTDNDENPD
                     LLECASLAMRVIQRGRLYFLRVWDRDSQRLKSFHGLNYFAVNPAWRVSAEFIFFDPPR
                     ETIVHTAVGSEMRVSIPGSARFQMSGVNCTLLGQAEGDELLFSFTDLTRQDATYPGGR
                     YLVTRRPVHNRLILDFNLAWNWPCAYTAYATCPLPPPENHLAMRVEAGEKRYHDE"
     gene            complement(16030..16515)
                     /locus_tag="PLW25_01760"
     CDS             complement(16030..16515)
                     /locus_tag="PLW25_01760"
                     /inference="COORDINATES: protein motif:HMM:NF023621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HOS53246.1"
                     /translation="MGVFSKILDKLGIKGKEEKAAAAAPAVKPEIPKTSALKPGGSVA
                     KDSRPVAGMVGAAGSGFKLDAFKPGAPAAVPMVDVVAKLEKLAAANPEKLDWKTSIVD
                     LMKLLGLDSSLAERKELAKELGCPEEKMADSAQMNMWLHKVVLQKIADNGGNVPKELL
                     D"
     gene            17051..18202
                     /gene="nagA"
                     /locus_tag="PLW25_01765"
     CDS             17051..18202
                     /gene="nagA"
                     /locus_tag="PLW25_01765"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOS53247.1"
                     /translation="MMKTHIRNAILLTPQQRMEHTVLTLSGGVIEKLTPEEEFSFEPG
                     NTLDAKGCYVAPGFIDLHFHGAMGVDTMDAVAAGMVTASAYCSQHGVTTFYPTTWSAS
                     QAEITRVINCVKECASHLPGARVGGVHLEGPYINPRRRGAQSLSFIRKPDAREYLPWL
                     ESGMVSILTCAPEVEGGEALIRNAVRLGARVAIGHSSASYEQVKAAANLGATQAAHLF
                     NGMDGLHHRHPGTAGGILDDDRLLAQVICDGVHLHPAVVRLVLKAKSPSRIALITDSI
                     RGAGRADAKYEGNGQFIQVKDGVARDAEGHLSGSTISMDLALRSLIAFTGCDLLEALV
                     MVTSAPAEEMGLGAKKGRLAPGYDADLVFLQKDLTVAATMVSGAFVYQC"
     gene            complement(18292..19314)
                     /gene="iolG"
                     /locus_tag="PLW25_01770"
     CDS             complement(18292..19314)
                     /gene="iolG"
                     /locus_tag="PLW25_01770"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HOS53248.1"
                     /translation="MSNQKVIRVGVIGAGRIGKIHAENLATRIPGVVVSAIADINQQA
                     AQALAEKLHVANAFSDYRKIIEDKDIDAVAVCSSTDTHADLIIAAANAGKHVFCEKPV
                     DHSLDRIDKAINAVSKAGVQCQIGFNRRFDPNFKKLHDLVQTGKIGDLHILRITSRDP
                     APPPAEYVKLSGGMFLDMTIHDFDMARFLSGSEVEEVYAIGEVRVDPAIGAAGDIDTA
                     VITLKFANGAIGTIDNSRKAVYGYDQRAEVFGSGGMAATANNTPNSSVYSNADGVVSE
                     KPLYFFLERYMESFVAEMRDFINAIREGNPTPVNVFDGRKPVVIAMAATRSMLENRPV
                     KLSEIE"
     gene            complement(19327..20229)
                     /locus_tag="PLW25_01775"
     CDS             complement(19327..20229)
                     /locus_tag="PLW25_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS53249.1"
                     /translation="MIRIANAPCSWGALEFDLQGKAAGYERVLDEMQRSGYAGTELGD
                     WGFMPTEPGKLKEELKKRELVLLGAFVPVALKDATTHPQGIESALKVARLLAAVEGSS
                     PFIVLADDNGKVPERTRNAGRVTPEMGLSDAEWQVFAEGTEKVADAVKRETGLRLVFH
                     HHCGGYVETPAEIDSLLRLTDPQLVGLCLDTGHYQFGGGDPLTFLQENAGRVWHMHFK
                     DCHPGIAERSRVLGWDYFKSVEQGVFCELGKGAVDFPAVKAELERQGFEGWIVVEQDV
                     LPGMGSPYESALRNREYLKSIGFN"
     gene            complement(20256..21272)
                     /gene="iolG"
                     /locus_tag="PLW25_01780"
     CDS             complement(20256..21272)
                     /gene="iolG"
                     /locus_tag="PLW25_01780"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HOS53250.1"
                     /translation="MAVKIGLIGAGRMGRVYASTLAFSVSEVDLVAVADPDRAALAEV
                     ADRYHIPSTYQDYRDLLDSQEIEAVVVASPTGTHAEVIAAAAQAGKHIFSEKPLSQNL
                     QRCDDALTAVRQAGVKLQMGFMRRFDAPYAAAKQKIRDGVIGKPVMFKSTSRDPKRTS
                     LEFAKRENSGGLILDMGVHDFDLARWLMDSEVARVHSEGACLAFPELKEVGDIDNVVI
                     NLRFTNDAIGNIDLSRNAVYGYDIRTEVLGTDGSLWIGYLQQTPTLVLTRAGVMHDTV
                     PYFMERFGLAYAEQVRVFVKHILEDTPPEVTGEDARAATAIGIAATLSLDEGRPVQVR
                     EVDL"
     gene            complement(21303..23168)
                     /gene="iolD"
                     /locus_tag="PLW25_01785"
     CDS             complement(21303..23168)
                     /gene="iolD"
                     /locus_tag="PLW25_01785"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HOS53251.1"
                     /translation="MTSTIRLTMAQALVRFLKNQYIERDGVETPFFAGMLGIFGHGNV
                     AGIGQALQENPDFRYIMVRNEQSAVHIASGFTKMHNRLQTFACTSSIGPGATNMVTGA
                     ALATINRLPVLLLPGDIFARRNVAPVLQQLESPNTQDISVNDCFKPVSRYWDRITRPD
                     QLLTSLPEALRVLTSPADTGAVTLSLPQDVQAEAYDYPLEFFRKRVWMVARPLADRAL
                     LKRAVEMIRSSKQPLIVAGGGVLFSGASQALDTFARQTGIPVGETQAGKGSLAYDHPQ
                     CLGAIGATGTLPANRIAHGADLVIGIGTRYSDFTTASKTAFQNLAVRFININLFEMDA
                     FKHSGLALTADARATLEELTSALAGYQVSADYATRIRELKHEWEVETDRLYHLRHQPL
                     PAQSEIIGAIWETIADGDVMMSAAGSQPGDLHKLWRARTPNSYHMEYGYSCMGYEIPA
                     SLGVKLAAPEREVYVWVGDGTYLMNPTEILTAAQEGLKVTIIVVDNAGFGSIGGLSAS
                     LGSSGFGTRFRKRDPQTGQLNGPALEVDFAANAASLGAEVFTPGSIEDFRKALAHTRS
                     LDHTSVIVIKADREVRVPGYESWWDVAVAEVSTMPEVQAARKDYEEHLKDEKYFL"
     gene            complement(23187..>23709)
                     /locus_tag="PLW25_01790"
     CDS             complement(23187..>23709)
                     /locus_tag="PLW25_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOS53252.1"
                     /translation="AEGKSIAEMSPVCTIMVGRTDDWMKVAAKKNAVDIDPAFLDWAG
                     IACFKRAYEIFKEKGYRPRLLAAAYRHLGHWSEFIGGDVILTIPYEWQLKINASDIEV
                     RPRMADPVDPVIVKALLSKIPEFRKAYEPDGMTVAQFDTYGATVRTLRGFISSYHELQ
                     GVVRDFMLPNPDL"
     assembly_gap    23710..23809
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23810..24072)
                     /locus_tag="PLW25_01795"
     CDS             complement(<23810..24072)
                     /locus_tag="PLW25_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOS53253.1"
                     /translation="MTTYKSKLHETVSTTPTDYWNDSCSIEELTYAVEHGAVGATTNP
                     TIVANVLKKEMNLWEERIHQIIAENPIWSEVEITWKLIEEMAVR"
     gene            complement(24084..24962)
                     /gene="iolB"
                     /locus_tag="PLW25_01800"
     CDS             complement(24084..24962)
                     /gene="iolB"
                     /locus_tag="PLW25_01800"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOS53254.1"
                     /translation="MKSTESSLLVKPDLTRAEYIRVTPQSVGWEHLSFCAVRLKQGEE
                     WHFNTENNELALVILGGTLDVHSNRGAWRSIGSRTDVFHGMPTTLYLSRDTDFTVTAA
                     YGAVDFACGWAAATKDFPARLVSPEEVKVELRGGENASRQINQMILPGFPCERLVVVE
                     VYTPSGNWSSYPPHKHDVRRVDENGRLLEADLEEIYFYKIDKPEGYAYQRCYTDDRRI
                     DEIMLVQNDHLVLSPEGYHPVVAAHGYTAYYLNILAGSDQSLASSDDPAYAWIKDTWK
                     SKDPRLPLVQLSMNYK"
     assembly_gap    25032..25131
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25132..25859)
                     /locus_tag="PLW25_01805"
     CDS             complement(<25132..25859)
                     /locus_tag="PLW25_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOS53255.1"
                     /translation="MGVTIKDIAKVAGVSHTTVSRALNGNPVIPPKTAAMIRNIAGDL
                     GYLPSAAARGLKTRRSGVLGVLVSRIDNPYFGEIIQGIEDAIRNSGYSIFIASSSLDP
                     QKEKEIVQAFSEHRVDGVIIGSVPVERSNLNLLNQYGIPAVVINNQSARSQKYSISHD
                     DEYGARQVTRHLLDLGHRRIAYLGNACAPRINDNRLQGLKAECESSGFTVAENWITHQ
                     PGGEIENGAAGMTALLGAGVPFSAV"
     gene            26095..26838
                     /locus_tag="PLW25_01810"
     CDS             26095..26838
                     /locus_tag="PLW25_01810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOS53256.1"
                     /translation="MNPDIEAIFLDVGNTLRIVLEEPDFQQNAMQELMRLVGTLESQE
                     AFFEKLETRWKAYRKASKASLLESPEEILWTKHLLPDYPAEKIAPLHGRLTRLWRDRD
                     GRRVPRHDAKETIIELHRRGYLLGIIANTITETEIPDWMEADGVTQYFKTVILSSKVG
                     IRKPDPEIYWMAARQIGVEPAKCAYLGDNPVRDVEGTRAAGFGMMILFDEPATLAKEP
                     PTGEHTPDYTIKEIRELLDIFPPRNRQVN"
     gene            26848..27582
                     /locus_tag="PLW25_01815"
     CDS             26848..27582
                     /locus_tag="PLW25_01815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOS53257.1"
                     /translation="MDIDAIFLDIGNTLRILMKEEPYREEARQKIAALVGTSQPPQSF
                     SEELDRRYKEYRKWAFEHLVEASEEELWTRWLAPEFPAQHIAPLAHELTYQFRQSMGR
                     RVMADGCKEVIHELHSRGYTLGIISNVITTSEIPDWLAAENLSRYFKSVLLSSTFGRR
                     KPDPSIYLEAARRAEVAPAKCAYVGDNFSRDVAGTRAAGFGMVIILPDPAEKDLRVAP
                     EQQPDLVIHSLWDLLDHFPPRNGHQE"
     gene            27585..29192
                     /locus_tag="PLW25_01820"
     CDS             27585..29192
                     /locus_tag="PLW25_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53258.1"
                     /translation="MPKSAGISLENTNRARGSAALAKTVKDLYERGQTDYNMEPIILQ
                     DAAGNAVGRIKDGDAVVFCCRRGEREIQLTEAFTDPQLNQFPREVFTHLPFVILTLYH
                     DKFKDLPVAFAPSKVQATLGEVVSQAGFSQVRIAESEKFAHVTFFLNGGNNQPYPQEE
                     DIRIPSLKGVPFDQYPELSLEKVAQAVIEAIHRGTGLVVTNFANGDVIGHTSNKAAKI
                     ACAQFVDRHLREVVEKANAAGYVVIITADHGNLEVLENADGSAHVSHTTNPVSCLIID
                     PAAAETVTPREGKLADIAPTVLSILRVTPPAQMTGKNLAPEHDFGVDRKVLLVILDGW
                     GISGVPENDPIAMAETPFWDSLLARYQHSLLEASGEAVGLKAGKAGNSEAGHINIGAG
                     RVVLQDDVRLDRAMQDGSFYTNEVICDVIEGVKARGKNLHLIGLLTEKSSHGSMEYPL
                     AVLKMAKDAGLQHVFLHMILDGRSTEPGSAPALLKKLEDLMNEIGVGQIVTGIGRGIA
                     LDRDGNYNKTQIAYDALVYGKGRGAGK"
     gene            29189..>29991
                     /locus_tag="PLW25_01825"
     CDS             29189..>29991
                     /locus_tag="PLW25_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53259.1"
                     /translation="MNYEFLKKLIQPADTKIVMLIMDGLGGLPKEPGGKTELETAHTP
                     HMDALAARSALGFSSPAGAGIAVGSGPGHLAIFGFDPIEHEIGRGALEALGVDIDLGA
                     EDIAARGNFCKVDANGILTDRRAGRLATPEAFRLAKMLNSIAIDGVQIQVEPIKEHRF
                     ALVMRGEGLAAKISDTDPLVEGVAPRQAVALDQASEKAARLVNAYIQKAQEILRSEEP
                     ANMVLLRGFDKLPVLPKYGELFKLCAASIAVNGMYKGVSRLVGMQVLPV"
BASE COUNT         7010 a         8106 c         8082 g         6493 t
ORIGIN      
        1 ggcggcgtat ccaccccgct catcgagcgc aacaccaccg ttcccacgcg gaaaagccag
       61 gtgttttcca ccgcgtctga tagccagacc caggtggaga tccacgtgct gcaaggagag
      121 cgccccatgg ccgcggacaa caagtcgttg ggcaagttca acctcgacgg cattcctcca
      181 gcgccacgcg gcgtcccgca gatcgaagtt accttcgata tcgatgccaa cggcatcctc
      241 aacgttaccg cgctggataa agcgaccaac cggtctcagc acatcaccat taccgcttcc
      301 agcggtctta cagaaagcga aatccagcaa atgaagcgtg acgcggaagc caacgctgag
      361 gctgatcgca aacgaaaaga actggtagaa gtgcgtaaca acgcggataa caccatttac
      421 gccgcggaaa aattgctccg tgaaaacagc gagaagattc ccgctgatac gaaaaaggaa
      481 gtcgaagagg ccattgccag cctgcgcggc ttgatgaaca atgaggatgc aggcgccatt
      541 cgcaccgcca ccgaagcact gggacagtcg atccagaaga ttggcgccag catgtaccag
      601 cagcagcctg aagccggtgg gcagggcgcg ccaggtgaac agcaggggca gcccggaact
      661 gacagcaacg gcggcaaccc tccagatgga gatgctggcg acgtggtgga tggcgaattt
      721 aagtctgttt agaccaggcg actgccatcc gttataatct gacggggcgc atggagaaat
      781 tttctccgcg ccccaggttc accttaaacc gcccacagga ataatgatgt atgaccaagc
      841 gtgattatta tgaagtactg ggtgtcaacc gtaacgcttc cgcggaggag cttaaatccg
      901 cgttccgcac cctggcgcgc aagtaccacc cggacatgaa caaagaagcc gactcagaag
      961 agaaattcaa ggaaatcaac gaggcttacg ccgtcctttc agaccccgat aagcgccgcg
     1021 cttatgacca gttcggtttc gctggcgtga atggcgcggg cggcgtgcct gattggacga
     1081 ccatggattt cgctgatatt tttggcgaat ttttcggctt cgccaccggt gcatcacgcg
     1141 cgcgtcagaa cgccccgcgg cgcggtcagg accttgctta tactgtcacc ctgacctttg
     1201 aagaagccat tttcggggtt gaaaaagagg tcgagattac ccgcgatgaa gtgtgccaca
     1261 cctgcggcgg taagggggct gaacccggca ccaacccggc gaaatgcagc aattgcgcgg
     1321 ggcgcggaga agtgcgccag gtgcggcaga cctttttggg gtccatggtg caggtaacca
     1381 cctgccctgt gtgcgccggg cgtggagaga tcatcggcac gccctgccac acctgcagcg
     1441 gccgggggct ggaacgcaag acgctcagca aaaaggttgc cattcccggg ggcgtggata
     1501 acggtacaca gatccggctg gctggtgaag gacagcccgg tatcaacggt ggacccactg
     1561 gcaacctcta tattgaaatc aaggtcaaaa gccacaagtt cttccgccgt aaagagaacg
     1621 acatcctgct cgacctgaac attaatatcg cccaggctgc gctcggcgcg gatgtcgaaa
     1681 tccccactgt ggatggtcca accaagctgg taattcccgc tggcacgcag ccaggcaaag
     1741 tgttcactct gcgcggcaaa ggcagcccca tcttgcgcag caacgcccgc ggtgatcaaa
     1801 aagtgatcgt caatgttgaa atccccacac gcctgacgga cgaacaacgc cagcacctcc
     1861 aggcgttggc agccagtatg ggcacggaag taaaaccgca ggaaaagggt tttctggacg
     1921 cgttgaagga ggtttttggt ggctgagggt aaatggctgc aggtttcgct caccgtggat
     1981 ggtgaactgg ctgaagccgt ggcagaagtg ttaaaccgtt ttaccagcgg cggagtggtg
     2041 gtggaatctg acgtgattta ccgcaacgct gaagatgaag gcacccctta cggcgcggtc
     2101 agagtgttcg gttatctcct catagacgag aatatcgagg taaaccgtca caagctggaa
     2161 gaagcgttgt ggcacctgcg cgccatacgt gaacttccgc agcccgaata ccgtgaaatc
     2221 gccgatgaaa actggatgga agcctggaaa gcgcattatc accctattcc cattggaaaa
     2281 aaactgttga ttttaccagc atggatcaag cagaaagatc aaagccggct ggctgtgaag
     2341 attgacccga gcatggcttt tggcaccggc acacatccct ccactcagct gtgcctggag
     2401 ctgcttgaag actacacagc gcccggaaaa ccggtcatcg atatcggctg cgggtctggc
     2461 atcctctcca tcgcggcgct caaactcggg gcgtctaagg ccatcggcgt ggatatcgac
     2521 gcgccagcca tcaaagccac acacgagaac agccgcgcca acggcgtgga agaaaaagta
     2581 gaaaccggac tggggtctgt tgaggaagta aaaagggggg atttttccgc gcgtcaggcg
     2641 ccgctggtgc tggcgaacat cctcgcgcct gtgctcatcc acctgtttga agtcggtatg
     2701 gcacagctgg tgtcgcctgg cggtgtcatc ctgctggccg gcatcctgtc tgaacagggc
     2761 gccagcgtgc gcgccgcggg tgaagcgcgc ggtctcactt ttgttgaaga gcgctgttgc
     2821 ggcgattggt cagcgctggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 cttttcggcg cgccgtgagc gaaacgattc acaaagtcag gataatgggt tttaccgtgc
     3001 aacaggtcat gccagatttt ctgctcatgc caggtcatat gtccgatgat ccagctgatg
     3061 cagttcatcg ggcccaggtg tttcaccgcg tcctcagcgg aaactccttc aagaccgcgc
     3121 tcaaactcac tgcgggtgaa gaacaattgt gcgaccagtg gatgtatcat ctgcaacctc
     3181 cttgctcaca gccctgctga accttttctt taatcataca actttcaacg tacgaacgaa
     3241 aacaaccctc agcataaaaa atggcggaga aacctggttc tctcgcaaag ccctattcaa
     3301 ggataatcaa cgaagtggtg atctcggctt tgcctttaag cgtagccgcc accgagcagt
     3361 acttcccttc tgaaagttcg atggcctgcg ctgcatcttt ttcggtgagc gcgctgccct
     3421 tcatgcggta agtgaggtgt atcttgcgaa aggtccaggg aggatcttgc tcctgctccg
     3481 cttcggcgct tatttccagg ctggtgagcc cggcgcgctt tttgttaagg atctccacca
     3541 catccaccgc gctgcaggaa gccagcgcca ccagcatcaa ctcggaaggc ttcattccca
     3601 ccccttcatc gggcgtggaa agcaccaccg tgtgcctggt ggagtccatg cccacgaact
     3661 gtttaccgcc aatccatttg actgaagctg aactcatgct gtcctcctgt tgctcatgtc
     3721 ttgcggatta ccgccagcat ttcgacggcc agttttcggt gttgctcaag gctgaggtca
     3781 tccgcgagca ggcagtcatt gacgatttgc ccggtataga catcaagggc ggcttgcaaa
     3841 gcagagcggg cggcgacaaa ctgctgcagg atgtcgctgc aatcgcgttc ttgcgcgagc
     3901 atgcggttga tgccgcggat ttgtccctcc agacggttga ggcggttgat cacctgcttt
     3961 ttgccattct ctgatgttac tttcatcggt atcctgtcac gcagggaggt ttaccccgct
     4021 caggtgaacc cgccagagcc tgagccacag ctgccggttg atactgcccc tcccgaagcg
     4081 ttgccggcgg atgagaacaa cgaaagcttt tttcgcgtat ctttgccggc gcagcgtggg
     4141 cagggagggc gctcgccagc ctggtcaaag cgcatcctct tttcaaattc atgtccacaa
     4201 tcagcgcaaa tgtactcgta taaaggcatg catcgctcct gaaacaatat actccagggg
     4261 agtattttat cacgcttcgc caccagtgca agggccgcct ggctaactgt aaaaggaagt
     4321 ttccctcttg acattgaaca tatgttcgtg tatgataata gtacaaatga ccgataaatc
     4381 gagcaggagg cagccaatgg cacgcagaca aaccggctta agtgagaggc accgcaagat
     4441 catggagttt ctgaccaggt ttcaggattc tcatggttat tccccctcca tccgccagat
     4501 cggggacagc attaacgtca aatcaacctc gctggtggat tactacctca accaactgca
     4561 aaacctgggc tacattgagc gagaagaccg catttcgcgc agcatccgcg tgctgcagcc
     4621 aattcaaccc ggggtctccc tgcccgcccg ggtaggagaa gctttccgcg gcgccgccag
     4681 cgcccttgcc agcatggtga gcatccctat cgccgggcgc atcgtggcca gcgaacctat
     4741 ccctatgcct gtttcagacc tcaactatta tgacgctgaa tccagcgtgg atatcgcgcg
     4801 cagtctgctg ccggcgaaag atgtcaacga tctcttcgcc ctcgaggtga gcggagattc
     4861 catgatcgat gccatgatca acgacggcga tatcgtcgtg atgaaaaagg cctcctcagc
     4921 caataatggc gagatggtag cgatctggct ggatgacaat aacgaaacca ccctgaagta
     4981 tttttacaaa gaagcagacc gtatccgcct gcagcccgcc aatcccaaca tggagcccat
     5041 tttcgtaaag aaccccaaga gcctgcgcat catgggcaag gtggtaatgg tcatccgcca
     5101 ggtgcattcg gtagcgggat aagccaatca gatacgcctg ctgctgccct gtttaaatta
     5161 actgcgtcca acctgcgttt ttatagatgt ggagcgcttt ccgctctacc catcattagc
     5221 agccggtagg ctgttggccc aaccccgtta tcctttctta tcaaccctcg ccctttcagc
     5281 cctggcgggt gatgaatcag gtgttaatat cagcagcggc ctcatcgtcc gggcaaaggg
     5341 ttggttgcaa gcccgcgcat ccacgttggt acacccgtgc caacaggcta tggaacgacc
     5401 aggatttcca cccacaacca acctaccggg taaccatccg gcatttgcag ctgccagtag
     5461 ccaatataat tgccggtcgt gtttggagcg accatatcca ccgacatttc gatttctttt
     5521 tttgaataaa cggtcgatcc caggagttgc ggcgaattgg cgctcatcgg ttccccgctg
     5581 ataaacacca gttgaaagtc acctgtccag taacaggatc cggtgttgag cagcttccac
     5641 gtcttggtaa aatgttcgcc cgcttttatt tcactgccgt cttcaaccgt aatatccgaa
     5701 gcctccaaac ccaggtaaca attcggatcc tggactgggg ttgaggtggg gatgggcgtg
     5761 ttggtgggcg gtatgggggt aaccgtaccc agggtgatgt cgtgtgtcgg ggtgggggta
     5821 atggtgggcg ggcgcatggc ctcaaccgtg gctttcagaa aatcagtgcc gattggtacg
     5881 accgccgggg tcgagccggt ctttccagcc agtgaacggg agcagcttaa ggttaggaga
     5941 gccagaataa ccagcagcgg gatgattttt cgggacattt tatttactcc gatggcgcga
     6001 ggatggcttg agaaacttgc caataaaacg tgtcaacagt ttaagtatac cggttttcaa
     6061 ttaccctgtg ggtcttgggc tgaaaggtta cacaaagaga ggacaatgcg ggcttattct
     6121 tctgaatatc cgcaaagcgc caaagcgcct ggcccggtac tgatacccag cacagggccg
     6181 gtgatgttca ggatcaattc tacagggttg aactcggggc ggatgcgttc tgccagcgcc
     6241 tcagcctctg ccagtgaatt tccgtgcaac accgcaatat gcaggcggtt gtggcttcgc
     6301 agctgggcga aaaatttctg atacaacatt tctaccagct gcttgtgagt cctcgccatg
     6361 gcggcgggtt ccaccaagcc ggaatgatga ttgatcgaaa ccagcggttg gatcttgagc
     6421 aggctgccca cccatttcac cgcgttgccg atgcggccac ttttctgcag gtactcaagt
     6481 gaatccatgg cgacatactg cactaacttt ttctttaagt catccagcat ggtcagtatc
     6541 tttttcactt ccatgcccgc gtcgcgagcg cgtgcagcag ccagcacttg ccagccgaga
     6601 ctcatggtcg gtccttttcc atccactacc gacacgggaa tcttcaccaa cctggctgct
     6661 tcgcacgcca tatggtatgc gccgctcatc gcgctgctga cggtgagggt gataatttca
     6721 gtagcccctt tggcggccac ttcttcatag gttttttgaa aatccgggat tcccgcctgg
     6781 gatgaagaag ggtagacagg gtcttcttcc aggcgtttgt aaaacgccaa cggagtcata
     6841 tccacccggt cgcggaactg ctcatctccc cagataatgt agtgagggat aatggttatt
     6901 ccatactgct cgatgagagc gtctgggatg tcacaggtgc tgtcagtgat aatgccgatc
     6961 atttcattcc ctcgcgattg tgaattgatt aatcataccc ccaaaagggc agccacgcac
     7021 gtttctaaac atcgagagtg tagaaacttg gatcattcac catacggcct tttccctttt
     7081 tccaactcct tcagggtatt tattcttttt gcacagtcat taaagcgcgt gagcaaccac
     7141 agggcataga tagctacccc gccaatgata accaaaaaac cggcaaccat ataggcgctg
     7201 gtatccggca tcattcacct tcttcctgga gcgcggcgcg cctctcgttg atttccgcct
     7261 gaaggttgcc caggcggtag cggtgccagt acagggcaat gaaaaggatg gaaaaggctg
     7321 ccaggctgct aaagaaggca gtctgcatgc tggggttcat ggcgaaaagc tcgttggcag
     7381 aagaatctgc cgagccaatc attaccgggt gaatggtccg aaaaacgcgg atggaaaaga
     7441 acgtaagcgg tacgctgatg aagccaacaa tcgcgtacac tgcagctagt ttcgcccgcc
     7501 gccgcgcatc atccaagcca ctgcgcaaca gcaggtaggc ggcgtagatc aattccatga
     7561 tgagcgcggt ggtcaggcgc gggtcccagg tccaccaggt attccacacc gggcgtgccc
     7621 aaatggagcc gctgacgatg catagcgcca tcaacaccat gccaatttca acccccgcca
     7681 accccgcgac atcccatttc aggctgcctg tcttcagaca ggctacagca gcgatagtcg
     7741 ccacaaaaaa tcccagcatc cccgcccaac cagcagccac gtgaaagtag aaaaccttct
     7801 gcacgctgcc catggtcaat tcaaaaggcg cgtagaatat caccagggcg aaagcggtag
     7861 ccaaagccag cagggcgaga aagtcaagaa aaattaaagc gcgaggagtc ttttcgggtt
     7921 tcattggtag ccaggtctat tcttcgataa ttctatcaaa caccagcaaa cccacagcag
     7981 caaaaagcag atcgtagccc accaacataa ccagccaggt atccagttcg gctgggttac
     8041 caccctgcaa gagaatcgtg gaagcgcgca cacaagccaa caacaagggg atgatcacag
     8101 ggaacagcaa taccggcagc agcatgtcac gcgcgcgcaa ttgcagggtc aacgtggaga
     8161 gcaacgtgcc tgtgaggatg taccccaggg ttcccagcaa gatcactccc aacccgcccc
     8221 aagagaaaag ggagaggtta ttcaacaatg catacaccgg cacgatgatg gcagcggtga
     8281 tgagcaggta aacccagttg accagcgctt tgccaaaaaa aatggctgtg cggtccaccg
     8341 gcgccaacag cagagcgttg atcgcttctc ggtctctttc caatgtcaaa gagcgattaa
     8401 ggctgatggt gccggcaaag cacaaagtca cccagatgac tccggctgct gccttgcggc
     8461 gtatctccac agagaggtcg aaagcgaaca caaacaccag tatgacggtc agagcgaaaa
     8521 ccagcatggt gccgagtatc tcgcgcgtgc gccgctccgc gagcaagtcc ttccagaata
     8581 tcgccaggat ggcgccgatg aagggcttca cgcgttcacc ccgtgccctt tgtggctgcc
     8641 agaaaagacg gtgagttcat taaaagttgc cctgagcgat ttcccgctga gtcccgcgct
     8701 ggggctcgac caggagattt ttccgtcatg caacaggtcg acgcgcgtgc tgatggcgac
     8761 tgcctgatta aaatcatggg tcgtcagcag caccatcttc ccgtcggcgg ctgcctggcg
     8821 aatgagcatt tcacacaact cggatgaacg ctggtcaagc ccggtatagg gttcatctaa
     8881 taacagcagg tcggggtcat gcagcagggc gcgggcgatc gccagtcgct gctgcattcc
     8941 gcgtgagaag gtgcgcacca gttcatccgc acggctgttc aaagcgacca tttccagcac
     9001 ctggcgaatt cgtttgtcgc tatcagcaat ctgatagaga cggctgaaaa agcgcagatt
     9061 ttcgcgcgca ctgaggttgt cataaagcat ggaggtatgc attaccacgc cgatgtggcg
     9121 acggtattca gcgggcgcgt tgagaggtgt gtatgagccg aagcgcacca tgccctgctc
     9181 cggccgcaag aggttggcca gcacgcgcag cagggtggtt ttaccagcgc cgttgatccc
     9241 gaccagtgaa acaatttcgg cgggttcaac cgtgagtgac agcccgcgta aaacttggcg
     9301 gttgccgaaa gacctggtga ggttgactgc gttgagcatc gcgcggcgcg tttcttcagg
     9361 cagagcctgg gggagaatca accattttca actcgcgcaa ggcaacagcc gcttcctgca
     9421 gcagcaaggt tcgctgttcc tcgtgctcct gctggctcat tttacccagc ccgaaatcga
     9481 aatccagctc gcggatgcgc tcgaggatgg cctggtaagc cgcctgtttg agcgctttca
     9541 catcaggcgc gtaggtgctg ccgcctaatg gttgatgggt gaagagcggg cgggtgatgt
     9601 aaaaaaacac cagcgcgagg aggataaccg tgataacaat tggtatgagc atcatcgcat
     9661 tttctgtgaa ctattttgcc agttccagca tggcgcggat ttcatcagcg gactgaaagg
     9721 gaccaatctg aaccgcagcc agcctgccat ctttgtcaac gaggtaagtc tcaggtacag
     9781 cctgcacctt gaaaagacgg gatattcgcg accccatgtc tggcccgttt ggaaacgtaa
     9841 tgccatattt ttcgagaaag gccttagcgt cattttccgt atccacataa ttcacgccca
     9901 ggaaaatgat atcatctgaa tccttcactt cccgccagac ttcttccagg atggcgcctt
     9961 cttcctcaca ggaagcgcac catgaagacc aaaagttgag caggatgacg cgcccatcca
    10021 tcctggattt ttgataatgt tcgcctgaga acgtcgtgat actgaaatca gggatctctg
    10081 cgccgacctc aaaaccggtc tgtcgtgact gtttcaacca caaccccagc gttataagca
    10141 atccaaccag taacaaccca aagatcacca tccagtacgg aaagcgtttt ttcgctttgt
    10201 tcacaggatc ctgcgtgaaa accgggagtg ttgtcactca acaccttcca ggtcattttc
    10261 aatctggctg atgaagtcct ctgcagccat tctacacggt ccttcatttg atacctcttg
    10321 agccgctgga attgtcttcg gcttgcggat gacccggatg gcgatcagaa tgcccgcgac
    10381 cagggtgagg ggtggaacca ggtaaatgag ccagttgaat ccgcgccgcg gcggaactgc
    10441 caacacctgg tctccatact gagtggcaaa ataggctttg atttcttcat cattccagcc
    10501 aagcgcgagc ttctcgcgga tcagttcgcg ccactgcgcg caggctttgg tgggacacac
    10561 ctccagcggc acgttttcgc acaccgggca atagagctca gaagccaggc ggttgacctc
    10621 atcatcactc accggcggtt cagtttgcgc gtatcccggc gtttgtacga tcaacaggaa
    10681 aaacagccat atcaccagaa gaacccgttt catttttcct cctggtgggt acgagggata
    10741 ttttttcgcc tggtcgtcgg ccacaacgcc accaacgtgc cgaaaaccag cacaccggag
    10801 ccaatccaca accagttgat caacgggttg cggtaaatct tgaaggtcgc tccttctgat
    10861 gaaattggct gccagtcaac caggatgaca tacaggtcat ctaccagggt gctgcgaact
    10921 cctggcaggg tgacagattg ctgtgatacg tagtaggtat cgctgcccgg gaaaacccgt
    10981 ttcaattccc ggttgtctct tttaatcatc accgaggctc gcgcgctgtt acgtccatct
    11041 gcattatcgg tcacctccag cttctcgtag gtgaaagtgt agccggaaag agacagcgtt
    11101 tcgcctgctg cgagcattcc ctgggtggtc gtctggaaaa actcgatgcc aataatgccg
    11161 agcgccatca aggcaacgcc gatatgaatg aggtatccac cgtagcgcct tgagaaacgt
    11221 ctgactaggg tgccaaatgc ctgcctcaca ggttcctggc ggttagatgc cctcagggtc
    11281 accgtccggt acgtatccag taccgtggtg aaaatggcca aggtgaccag ccacaccgcc
    11341 agcagtgcga accagttacg tacacccgcg agaaaaacga taagcgggac cgccagcgca
    11401 gccaaagcgg gtttccacag gtgccgctcc aggtttttgc tggttgagac accccacatg
    11461 gtaagcggaa cgatggccat caatagcaat aacaatccaa acagtggggc agtggcgctt
    11521 tcataaaaag gcggcccaac tgtgaccttc tgacctgtaa gcagttctga gaagataggg
    11581 aaaagcacgc cggtaaggca gataagaagg agcgcggtga acagcaggtt attgaaaaga
    11641 aaagccgatt cgcgcgagaa aaccgagtgt atctggttct ccgaacgcag cgcagaccag
    11701 cgataaagca gcaacccgaa agaaatgctg aacatgagaa tgataaacac gaagaacgcc
    11761 gggccaatcg cagattgaga aaaagcatgc actgatgaaa gcatcccaga acgggtgaga
    11821 aaagtaccca aaatgaccag gcaaaaagtg agaacaatga gggcgatgtt ccaatgctga
    11881 aaaaggcggc gcttttcctg ggtgatggct gaatgcaggt aagcagtgcc cgttagccag
    11941 ggcatcagcg ctgaaatttc cacaggatcc cagccccagt accctcccca accaagcaca
    12001 tcataagccc agcggctgcc cagcaccagc cccagtgaaa ggaagagcca cgccagcaaa
    12061 gcccatttgc gcgtggattt gagccagtga tcatcggtac gaccgcttac caatgcggcg
    12121 aaggcgtaag caaagggaat cacaaacccg gtgaaaccga ggtaaagcgc cggcgggtga
    12181 atgatcatgc cgaaatgccg taacaacggg ttcagccccg tgccgtctgt gggcttagcc
    12241 aatatggaca tcgccggtgg gaagaacgcg cgttttatga tcccgtcaag ggtaatgaaa
    12301 taacgcaaaa aaggattttc aaaaaagagt acaagaatga taaagaacag caacgtaaag
    12361 gaagtggccg caatgaccca gggcaacagg tctgcctcgt ttttccagcg gcgcgccaca
    12421 actggaaagg taaatcctgc gagcagccag caccaaaaca gcaacgaccc ttcctgcccg
    12481 ccccaaagcg cggcgagctt gaggtagagc ggcatggata ggtcggtggt ttggtaaacg
    12541 gaagagtatt cgaaacgccc gctggcgagc aggtaaagca gcgccagtat ggaaagagac
    12601 agcaccggga aggtgattag cgcagcacgc cgcgcagttt caatcagtgc atcagagcgc
    12661 gaacgacctc cacagagaga ggttatcacc gccgtcagtg tagttacaag ggcgataact
    12721 aacccgccat acccgatacc tccaatcacg gcaccgcctg tgcaggtact tccgcttcat
    12781 agcgagaggg acatttaagc aataactcct cagcgttgaa caccccatca tcgcccatgg
    12841 cgccggtcaa tattgcctgg gcttcatttt tcaacaggtc aggcataacg ccgttatagc
    12901 gcacctgtaa gcgagctgcc tggggatcga gcgaggcgtt atgcagcaca ttggccaacc
    12961 ctccacctgc ttcgatttcg cgattgtcac cgggaatgtg cgcgatggtg aaggtaaggt
    13021 caagagtttg agggtcatat tggatggtat cacccagcac cacacccgaa acccgcacgt
    13081 tggcgttatt cacccctttg ccggatgcca gttcttctac cgtgaggtaa tactgggcgt
    13141 tgcctttaag gctggtaatc accagcagca ccaccgccgc caggagcagc ataccgccga
    13201 tggcgaactt atggatcttg aagcgagggt gaggttcaac cgcgcgcggg cttttgttca
    13261 agcgttctcc taagggttat gtttgaaaaa accaagccac tgtgagggcg gtttttgttc
    13321 ggtgcattta ttcgcgctcc gcaggcggtt cgggcgcctc gtggagatct ggagactcgc
    13381 gatttatatc tgcctgattg tcttttctgg gttcttctac ctctttgatt aaatccgcta
    13441 ctgagcgggt cagagagctt tggacccccc tggattgacg gtctaagtct ctgatcgttt
    13501 catcgaggtt ggcttccctg gccagcattc tgggaatttc gtttatttca cgcctggtcg
    13561 cccatacttc ctgccagtat ggtgacctgg tgatacggcg aataaatgcg cccacttccc
    13621 gggcggactt caccatattg ccaggaccca ggagtatcaa cgccaggacg acaatgaaga
    13681 tgatctcagg cagaccaagg ttgaagaatt tcatatcact tttgctcttg tccctgtaaa
    13741 gggttgtgag agggcgggcg tatcattcct gcgttgagtc gtctttttct tccttttctt
    13801 cacctttcag gcctttccga aaagcccgga tggccccgcc aatctcgccg ccaatcttac
    13861 tgatcttgcc aacaccgaac agcagcgcga tgatgaccag gatgataatg agttctggta
    13921 ctccgagttt ggaaaacata tttcgctcct tgtcttgcga ttattttact attagttgct
    13981 tcctggtttg ctggttaacc agcaccggcg ccgtcagcct gctgagattt ttttcggtta
    14041 tgcccggcgg cgaccgcaaa caggatactg atcagataga gggcgcaaag aggaatcatc
    14101 atcaacccca tgttcaccgg atctggggtg ggggtgatga acgcagccag aatagcaatg
    14161 ataacgatgg caatgcgcca ttgcctggcc agttgtttgg cagtgaccag cccgatgcgc
    14221 gccagtagaa aaacgataag cggcagttca aaagccaatc ccacccaaaa gagcaggttt
    14281 aaaacaaacc caaagtacgt gctgggccgg ggagcagtgg cgatgcccat aaaattcagt
    14341 aaaaaaggta gagcagtggg aagtaaaacg aagtaggcaa aaaccacacc tgcagcgaaa
    14401 aagagggtcg ccagcgggat catgaaccat acccacttgc gttcgtttgg tttcaatccc
    14461 ggcaaaacaa atgcgaagat ttcgtacacc acgattggta gcgccagcac cacgccagca
    14521 agcagcgaga ttttcataaa ggcggtcacg ttttcggtga catcgataga tttcatcgcc
    14581 gagagtccgc caattggacc agccagaacc tctgccagtt ggcggctgaa gacaaagcca
    14641 attacagtgg ccaccgccaa ggcgatcacg ctgatgagca ggcgcttgcg cagctcattc
    14701 acatggctca atatgctcat tccagaagtt tcttgttcca tttatccaat aaccttcgtt
    14761 tagctgttaa ttgtaacagg gctatagggc agaacaagga cacttccctg tatttagcgt
    14821 atcagcgcta gcctgtggaa gtggatggtg aattcggtgg cacgcccgtt acaacagacg
    14881 aacaggctgg ttttatgggt tgtcattatt ttaatggttt cgcgtcttgc aggcgcggtg
    14941 aattcagtag tgggtgcaac tggcaatcaa acagggtgcg gtcaggtgag tgtgttattc
    15001 atcatgatag cgtttttcgc cggcttcaac gcgcatcgcc aggtgattct ctggcggcgg
    15061 cagggggcag gtggcgtaag cagtgtacgc gcagggccag ttccacgcta ggttgaaatc
    15121 cagtatcagc cggttgtgaa ctggccgccg ggtgaccagg tagcgcccgc cggggtaggt
    15181 ggcgtcttgc ctggtgaggt ctgtgaaact gaagagcagt tcatccccct ctgcttggcc
    15241 cagcagcgtg caattgacgc cgctcatttg aaagcgggca ctccctggga tgctgaccct
    15301 catttcacta ccaacggcgg tgtgaacgat cgtctctctg ggcgggtcaa aaaagatgaa
    15361 ttctgctgag actcgccagg cggggttgac tgcgaaataa ttcaagccat gaaaactctt
    15421 cagccgctgc gaatcacggt cccaaacacg taaaaaatac agcctgccgc gctgaatgac
    15481 cctcatggct agcgaagcgc attccagcaa gtcaggattt tcgtcgttat cggtgcgcag
    15541 ctggcgttca gcagcaggag cgccattaat cgtaaagtgg ctgcccacct ctacccagat
    15601 ttcatctcct gtgagggtga tcaaagcttc ggcgccctgg gcaagttccg ggatgtggat
    15661 agtgtagcct gagccatgac ccaatctgtt ttcgcccgtt tgcaacgggt acagcccgac
    15721 cagtgaaaac cagttcaagg gggattgcag caggttctgt tcacgcttcc ggcgttctct
    15781 ctgaatcatc ttctcatacg aggtgtgtgt tttcatgggt aaccgttcag gcgtattatt
    15841 tttagcgtta acttttagga gtgatcgaaa aggaagcagc aggatggtta agtcctgctg
    15901 cttgtaccga tcactcgtat tcaataggct tgtggggttc ttcgggttct gtttacccca
    15961 attgaatact ggttccgggt catttgataa aaatcaatag aaccggatcg tgggatgggt
    16021 atacttcact cagtcgagca gttccttcgg cacgttgccg ccgttgtccg cgatcttctg
    16081 aaggacaact ttgtgcagcc acatgttcat ttgggctgaa tcagccatct tttcctctgg
    16141 gcagccaagt tccttggcca gttccttgcg ttcagcaagg ctgctgtcaa ggcccaagag
    16201 tttcatcagg tctacgatcg acgtcttcca atcaagtttc tctggatttg cggcggccag
    16261 tttttcaagt ttggcgacca catccaccat ggggactgcc gcgggggcac ccggcttgaa
    16321 agcatcgagc ttaaagccgg agcctgcggc tccaaccatc cctgccactg ggcgggaatc
    16381 cttcgccact gagccgcctg gtttaagcgc gctggtcttc gggatttcag gttttacggc
    16441 aggtgcagca gcggctgctt tttcctcctt acccttgatg cccaacttgt cgagaatttt
    16501 agagaatact cccattttta ttcctttcca ctcattttaa tcagaacccc aactagggtt
    16561 aattatagac ggtttttgca agaaaagtta atattaacca gccaattttc atctgttatt
    16621 ttataaaatc attcatacca taaagcccac ttcaccatcc accctgtgta ttctcctgga
    16681 atttcattgc aacgctaccg cggttcaagc gtatactcat ggtatagatg ataaaatcgt
    16741 ttcgaaaatc acagcaataa ggagaagaaa agatgggaga ggtaggcaag aaagacaagg
    16801 gcaaaaaaga acaaaagaaa aaaccgaagt taaatatcaa ggaaaagaga aaacagaaac
    16861 aggagaaaaa gagcggtacc gcttaatgaa cttaagcgca gcgctggctt ttaacctgct
    16921 ttgcgggcaa ctcaatcatc tggtaattcc ggtcatcctc atggctggag tagtattccg
    16981 ttgcctgata ttcctgcacg attcttgaat cagtccccat ccgtttttca cccgcttaac
    17041 tgaccggctt atgatgaaga cccatatccg taacgccatc ctgctgaccc ctcagcaacg
    17101 aatggaacat accgttctca ctctgagcgg tggggttatc gagaaactga ccccggagga
    17161 ggaattctca tttgagcccg ggaatacgct cgacgcgaag ggctgttatg tcgcgccggg
    17221 gttcattgac ttacattttc atggcgccat gggggttgat acaatggacg cagtagctgc
    17281 gggtatggtg accgcatcag cgtattgctc tcaacacggc gtaaccactt tttaccccac
    17341 cacctggagc gccagccagg cagaaattac acgtgtgatc aactgtgtga aggagtgcgc
    17401 cagtcactta cccggcgcgc gtgttggcgg ggttcacctg gaaggtccct acatcaaccc
    17461 acgcaggcgg ggagcgcagt cactctcttt tatccgtaaa ccagacgcgc gtgagtacct
    17521 gccctggctg gagtccggta tggtcagcat tctcacctgc gcgccagaag tggaaggtgg
    17581 cgaagcgttg attcggaacg cggtgcggtt gggggcgcgt gtggccatcg ggcattccag
    17641 cgccagctac gagcaggtga aagccgccgc gaacctcggc gcgacgcagg cagctcacct
    17701 gttcaacggt atggatggac tgcatcaccg ccacccaggc acagccggag gtattctgga
    17761 tgatgaccgt ttgctggcgc aggtgatttg tgatggcgtt catcttcacc ccgcggtagt
    17821 gaggcttgtt ctcaaagcca aatcaccttc ccgcatcgcg ttgatcacgg attccatcag
    17881 gggagcaggg cgcgctgacg caaagtatga gggtaacggg cagttcatcc aggtgaaaga
    17941 cggggtcgcc cgtgatgctg agggtcacct gtccggcagt acaatctcta tggacttggc
    18001 gctgcgcagc ctgatcgcct tcacgggctg tgatttgctg gaggctttgg tgatggttac
    18061 cagcgccccg gcggaagaga tgggcttagg cgcgaaaaaa ggaaggttgg caccgggtta
    18121 tgacgcggac ctggttttct tgcaaaaaga cttaacggtt gccgcgacga tggtctcggg
    18181 cgctttcgtt taccagtgct gaacaacaat caatacaccc gcaagatagg cggcgggaag
    18241 acatcctcaa ccccgggagg tctgctacct cccgaggtta ttttatgcct gctattcgat
    18301 ctccgatagt ttgaccggtc ggttttccaa catcgagcgg gtggctgcca tggcaatgac
    18361 caccggtttg cgcccatcga acacgttcac cggggtcggg ttgccctcgc ggatagcgtt
    18421 aataaaatcg cgcatttccg ccacgaacga ttccatgtag cgctccagga agaaatagag
    18481 tggtttttct gaaaccacac catccgcatt gctgtacacg ctggagttgg gggtattgtt
    18541 ggcagtagcg gccatgccac cagaaccaaa aacttccgcg cgctggtcgt aaccataaac
    18601 cgctttgcgg ctgttatcaa tcgtgccgat ggcgccgttg gcaaacttga gcgtgataac
    18661 agcggtatcg atatcacccg ccgcgccaat agccgggtcg accctcacct cgccgatcgc
    18721 atacacttct tctacctcgc tgccggagag aaaacgcgcc atgtcaaaat catgaatggt
    18781 catatccagg aacatgccgc cggagagctt cacgtactcc gctggagggg gggccgggtc
    18841 gcggctggtg atacgcagga tatgcaggtc gccgattttg cctgtttgaa ccaggtcgtg
    18901 cagcttttta aagttggggt caaatcggcg attgaagcca atttggcatt gtacccccgc
    18961 cttactgacc gcgttgatgg ctttatcgat cctatccagg ctgtggtcta ccggcttctc
    19021 gcaaaacacg tgcttgccgg cattggcagc ggcgatgatg agatcagcgt gggtgtcggt
    19081 agaagagcac accgccactg catctatgtc cttgtcttcg atgatctttc gataatctga
    19141 aaaagcgttg gcgacgtgca gcttctccgc cagcgcctga gccgcctgct ggtttatatc
    19201 agcaatcgcg gaaacgacca cgccagggat gcgcgtggcc aggttttcag cgtgaatttt
    19261 tccgatgcgc ccggcgccaa tgacccctac tcgaattacc ttttgattgc tcattcttct
    19321 ccttgatcaa ttgaacccga tggatttgag gtattcacga tttctaagcg ctgattcgta
    19381 gggactgccc atgccgggta acacatcctg ttccaccacg atccagcctt caaatccctg
    19441 ccgttccagc tcagccttca ccgccgggaa gtcaaccgcg cctttgccca gttcgcagaa
    19501 aacaccttgc tcgacggact taaaataatc ccaccccagc acgcgtgatc gttcagcaat
    19561 accgggatgg caatccttaa agtgcatgtg ccataccctg ccagcgtttt cttgtaaaaa
    19621 agtcagcggg tcaccaccgc cgaattgata atgaccggta tccaggcaaa gcccaaccag
    19681 ttgtgggtct gtgagcctta gcagcgagtc aatctcagcc ggggtctcaa cgtatccgcc
    19741 gcagtgatga tgaaagacca accgcaaccc agtctctctt ttgacagcat ctgcgacctt
    19801 ctcagtgcct tcagcaaaca cctgccattc agcatcgctg agccccatct caggggtcac
    19861 acgcccagca ttgcgtgtgc gctccggcac cttgccattg tcatccgcca gcacgatgaa
    19921 aggggaactg ccttccaccg ctgccagcag cctggcaacc tttaaggcgc tttcaatgcc
    19981 ctgtgggtgg gtagtagcgt ctttaagcgc caccggtaca aatgcgccca gaagcaccag
    20041 ctcacgcttt ttcaattctt cttttaattt tccaggctct gtcggcataa aaccccagtc
    20101 acccagttca gtaccggcgt aaccggagcg ctgcatttca tcgagcacgc gttcatagcc
    20161 ggcagctttg ccctgcaggt cgaattccag tgctccccag ctgcatggtg cgtttgctat
    20221 tctgatcata cttcctccaa aaaagataag gtgaattaca ggtccacttc acgcacctgc
    20281 accgggcgtc cttcatccaa cgaaagggtg gcggcaatgc caatagcggt agccgcgcgc
    20341 gcgtcttctc cggtaacctc gggcggtgtg tcttccaaaa tgtgcttcac gaacacccgc
    20401 acctgctcgg cgtaggccaa gccaaaccgc tccataaagt aaggcactgt atcgtgcatg
    20461 acccctgcgc gggtcagcac cagggttggg gtttgttgca ggtagccgat ccaaaggctg
    20521 ccatctgtgc ccagtacctc ggtgcggatg tcgtaaccat aaacggcgtt gcggcttaaa
    20581 tcgatgttgc caatcgcatc attggtaaag cgcagattga tgacaacatt gtcgatatct
    20641 cccacctctt taagctctgg aaaagccagg caggcgcctt cgctatgcac acgggcgact
    20701 tcgctgtcca tcagccaacg cgctaaatcg aaatcgtgca cgcccatatc caggatcaac
    20761 ccgccgctat tctcacgttt ggcaaactcc aggctggtgc gcttggggtc gcggctggtg
    20821 gatttgaaca tcaccggttt accgatgacg ccatcgcgta ttttttgttt cgctgcggcg
    20881 taaggggcgt caaagcggcg cataaagccc atctgcagtt taaccccggc ctgcctgacc
    20941 gctgtcagcg catcatcgca acgctgaagg ttctgcgaaa gcggtttttc gctaaagata
    21001 tgcttgcccg cctgcgccgc ggcggcgatc acttcggcgt gcgtgccggt gggagaggcc
    21061 acaacaacgg cctcgatctc ctggctgtct aacaggtcgc gataatcttg gtaagtggaa
    21121 gggatgtggt agcgatccgc cacctcggcg agcgcagcgc ggtcaggatc agccactgca
    21181 accaggtcca cttcggatac tgagaacgcc agcgtgctgg cataaaccct gcccattctt
    21241 cctgcgccga tcaatcctat cttcaccgcc atcgttcacc tcgcgctttt tctatctcca
    21301 tatcaaagga aatatttctc gtctttcagg tgctcctcgt aatctttacg cgccgcctga
    21361 acctcaggca tggttgaaac ctctgccaca gccacgtccc accaggattc gtaacccggt
    21421 accctcacct cgcggtctgc tttaatgaca atcaccgagg tatggtcaag tgacctggta
    21481 tgagcgagag ctttacgaaa gtcctcgatg gaaccaggtg taaatacctc agcgcccagg
    21541 ctggcggcgt tggcagcaaa atccacctcc agcgcagggc cattaagctg accggtttgc
    21601 gggtcacgtt tcctaaaacg agttccgaag ccagagctgc ccaaagaggc actcaagccg
    21661 cctatcgacc caaaaccagc gttatcgacc acgatgatgg ttaccttcaa tccctcctgc
    21721 gcagcggtga ggatctcggt gggattcatc aggtaggtgc catcgcccac ccacacgtac
    21781 acctcgcgct caggcgctgc cagcttcaca cccagcgagg caggaatttc atatcccatg
    21841 caggagtaac catactccat gtgataggag ttgggcgtgc gcgcacgcca caacttgtgc
    21901 aggtcgccgg gttggctgcc cgcggccgac atcatcacat cgccgtcggc aatcgtttcc
    21961 catatggcgc caatgatctc gctctgagcg ggtaaaggct ggtgacggag gtgatacaaa
    22021 cggtcggttt caacttccca ttcgtgtttg agttcgcgaa tgcgggtggc gtaatcggct
    22081 gatacctggt agccagcgag agccgaggtc aattcttcca gcgtcgcacg cgcgtctgcg
    22141 gtgagcgcca accctgaatg cttaaaagcg tccatctcaa acaggttgat attgatgaag
    22201 cgcactgcga gattctgaaa agcggtttta ctggcagtgg tgaaatcaga gtatcgtgtg
    22261 ccaatgccaa tcaccaagtc ggcgccatgc gcgatgcggt tggcgggcaa cgtgccagtg
    22321 gcgccaatcg cacccaggca ctgggggtgg tcataagcca gtgaaccctt gcccgcctga
    22381 gtctctccca cgggaatgcc ggtttgcctg gcgaaggtgt caagcgcctg gctggcgccg
    22441 ctgaacagca ccccaccgcc ggcgacgatc agtggctgtt tggatgaacg aatcatttca
    22501 accgcgcgtt ttaaaagcgc gcggtctgcc agcgggcgcg cgaccatcca aacacgtttg
    22561 cggaagaatt caagcgggta gtcgtaggct tcagcctgca catcctgcgg cagcgagagc
    22621 gtcaccgctc cggtatccgc aggggaggtg agcacgcgca gggcttcagg taaagaggtg
    22681 agcagttggt cagggcgggt gatacggtcc cagtagcgcg atacaggttt gaagcaatca
    22741 ttcacgctga tgtcctgcgt attgggagat tccagctgtt gcagcactgg ggccacgtta
    22801 cgacgcgcaa aaatatctcc gggcagcagc agcacaggca ggcggttgat ggtggccaac
    22861 gccgccccgg tgaccatgtt ggtggcgcca ggaccaatgg acgatgtgca ggcgaaggtt
    22921 tgcaggcggt tgtgcatttt ggtaaagcca ctggcgatat gcacagcgga ttgttcgtta
    22981 cgtaccatga tgtagcgaaa atcagggttt tcttgcagcg cctggccgat acctgccacg
    23041 ttgccgtgac caaagattcc cagcatacca gcgaagaaag gggtttcaac tccgtcccgt
    23101 tcgatatact ggttcttaag gaagcggaca agggcttgcg ccattgtaag tcggatggtg
    23161 gaggtcattt ctctcctttc aacgggctac agatcggggt tggggagcat gaaatcgcgt
    23221 actactccct gcagttcatg gtaagaactg ataaatccgc gcaaggttct cacagtggca
    23281 ccgtaggtgt caaattgcgc tactgtcatc ccatccggct cgtacgcctt gcggaattca
    23341 ggtattttgc tgagcagcgc cttaacgatg acggggtcga ctggatctgc catgcgcgga
    23401 cgaacttcga tatcgctggc gttaatcttg agctgccatt cataagggat ggtgaggatg
    23461 acatcaccgc caatgaactc agaccagtgt cccaggtggc gataagctgc tgccagcagc
    23521 ctggggcggt accctttttc cttgaaaatt tcataagcgc gtttaaagca ggcgataccc
    23581 gcccaatcga ggaaagcagg gtcaatatca accgcgttct ttttggccgc taccttcatc
    23641 cagtcatccg tgcgccccac cattatggtg catacgggcg acatctcagc gatggatttc
    23701 ccttcagcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gcacagccat
    23821 ttcctcgatc aatttccagg tgatctccac ctcagaccag atcgggtttt ccgcaatgat
    23881 ctgatgaata cgctcttccc acaggttcat ttccttttta agcacattgg ccacgattgt
    23941 ggggttggta gtggcaccaa ccgcgccgtg ttcaaccgcg taagttaatt cttctatgga
    24001 acaggagtca ttccagtaat cggtgggggt cgttgagact gtctcgtgca atttgctctt
    24061 ataggtggtc atttttcact cctttacttg tagttcatgg ataattggac aagcggcagg
    24121 cgcggatcct tgctcttcca ggtgtctttg atccaggcgt atgccgggtc gtcgctgctg
    24181 gcaagagact ggtctgagcc cgcgagaatg ttcaggtagt aagccgtgta accgtgggca
    24241 gccaccacgg ggtgatatcc ctcgggtgat aagacgaggt ggtcattttg caccagcata
    24301 atttcgtcga tgcggcggtc atctgtgtaa cagcgctggt aagcgtaacc ttcaggttta
    24361 tcgattttgt aaaaataaat ttcttccagg tcagcctcca gcagccggcc attttcatcc
    24421 acgcgacgca catcgtgctt gtgcggtggg taagagctcc agttgccaga tggcgtgtaa
    24481 acctctacca ccaccaggcg ctcacacgga aaacccggaa gaatcatctg gttgatctgg
    24541 cgtgaagcgt tctcgccgcc gcgcagttca actttcacct cttcagggct gaccaaccgc
    24601 gccggaaaat cttttgtagc ggcagcccag ccgcaggcga aatcgaccgc accgtaagcg
    24661 gccgtaacgg tgaaatcggt atcgcgggat aggtaaaggg tggtaggcat gccatgaaac
    24721 acatctgtcc ggctgccaat ggatcgccac gctccgcggt tcgagtgcac atcaagcgtt
    24781 ccgccaagga tgaccagcgc cagttcgttg ttttcagtat taaaatgcca ttcctcgcct
    24841 tgtttaagcc tgacagcgca gaaagacaaa tgttcccagc caacgctttg cggggtcacc
    24901 cggatatact ctgcacgggt gagatctggt ttaaccagta aagaagattc ggtagatttc
    24961 actttttact cctttgcaaa ttgagcggtc gattttctta ccagcaattc accgcgcatc
    25021 atcttattga gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacggctgaa
    25141 aagggaacac cggcgccgag gagggcggtc ataccggccg cgccgttttc aatttcacca
    25201 ccaggttggt gggtaatcca gttttctgcc accgtaaaac ccgatgactc gcattcagcc
    25261 tttaatccct gtaagcggtt atcgttgatg cgcggagcgc aggcgtttcc cagataagcg
    25321 atgcgtcggt ggccgaggtc aagcaggtgg cgcgtgacct gcctggcgcc atattcatca
    25381 tcgtgcgaaa tcgagtattt ttgcgaacgc gcagactggt tattgatgac cactgccggg
    25441 attccatact ggttaagcag gttgagattg ctgcgctcaa ccggtaccga accaatgatg
    25501 accccgtcca cccgatgttc actgaaggct tgaacaatct ctttttcttt ttgtgggtcg
    25561 agagaagacg aggcaatgaa aatgctgtat ccgctatttc tgatggcgtc ttcaatgccc
    25621 tggatgatct cgccaaaata gggattgtca atgcggctga ccagcacccc taacaccccg
    25681 gaacgcctgg ttttaagccc ccgggcagcg gcactgggca ggtagcccag gtctccggcg
    25741 atattccgga tcatggcggc ggttttgggc gggatgacag ggttgccatt gagcgcccgc
    25801 gagactgtgg tgtgtgaaac gccagcgacc tttgcaatat cttttatcgt gacacccata
    25861 acactccatt agcgcacacg tgtgcataaa catcatagca aattgcctta tgaatgtcaa
    25921 cttaaaaaat gtaagaatac ttgcaaaatt aatttaaatg ttgtattatc atacatgtaa
    25981 gtaatcctgc aaaaaacatt gaaatgttag gccagcaatg catttcgcct gagcctcacc
    26041 gccctggaca cgcctgaact tctataagaa tcgacctcgt gaaaggatat ggatatgaac
    26101 cctgacatcg aagccatctt tctggacgta ggcaatacgt tacgcatcgt gctggaagag
    26161 cctgatttcc aacagaacgc catgcaggag ttaatgcgcc tggttggaac gctagaatcc
    26221 caggaagcct tctttgaaaa actggagacc cgctggaaag cttaccgtaa agcctccaag
    26281 gcgtctttgc tggaatcgcc cgaggaaatt ttatggacaa aacatctgct gcccgactac
    26341 ccggcggaaa agatagcccc cctgcacggc aggctaacgc gcctttggcg cgaccgggat
    26401 ggacggcgcg ttccgcggca cgacgccaaa gagaccatca ttgagctgca ccgccggggt
    26461 tacctgctgg gcattatcgc caacaccatc actgaaacgg agattcccga ttggatggaa
    26521 gcggatggtg tgacccagta tttcaaaacc gtcatccttt cctccaaagt gggcattcgc
    26581 aaaccagacc ccgaaatcta ttggatggca gcgcgccaaa ttggcgtgga acccgccaaa
    26641 tgcgcctacc tgggcgacaa cccggtgcgc gatgtggaag gaacgcgcgc ggccgggttc
    26701 ggcatgatga tccttttcga tgaacccgcc acactggcaa aagaaccacc cacgggtgag
    26761 cacactcccg attacaccat caaagagatc agggaactat tagatatctt cccgccgcga
    26821 aaccgccagg tgaattaaag cacccacatg gatatcgacg ccatttttct cgacatcggc
    26881 aacaccttac ggatattgat gaaagaagag ccgtatcgtg aagaagcgcg tcagaaaatt
    26941 gctgcgctgg ttggcacaag ccaaccgccg caatcgttca gcgaggagct ggatcggcgt
    27001 tacaaggaat accgcaagtg ggcttttgaa cacctggttg aggcttcaga agaagaattg
    27061 tggacgcgct ggctggcgcc tgagttccca gcccaacaca tcgcgccact ggcgcacgaa
    27121 ctcacctacc agttccgcca atccatgggg cggcgggtga tggcggatgg ctgcaaggaa
    27181 gtcattcatg aattgcacag ccgcggttac acgctgggaa tcatcagcaa tgtcatcact
    27241 accagcgaga ttcccgattg gctcgccgct gagaatcttt cgcggtattt caaatccgtc
    27301 cttctctcct ctaccttcgg gcggcgtaaa ccagatccat ccatctatct ggaagccgcc
    27361 cgaagggctg aagttgcacc agcaaaatgc gcctacgtgg gcgacaattt ctctcgtgat
    27421 gtagctggaa ccagggcagc cggctttggc atggtgatca tcctgccaga cccggcggaa
    27481 aaagacctca gggttgcgcc ggagcaacaa ccagatctgg tgattcacag cctgtgggac
    27541 ttgctggatc actttccgcc cagaaatgga caccaggagt aaatatgccg aaatcagcag
    27601 gtatttcact cgaaaacaca aatcgcgcca gaggaagtgc ggcgctggca aaaacggtga
    27661 aggacctgta cgagcggggg caaaccgatt acaacatgga gccgatcatc ctgcaagacg
    27721 cggcaggaaa tgcggtcggt cggatcaaag atggcgatgc ggtggtcttc tgctgccgcc
    27781 gtggagagcg tgaaatccaa ctgaccgaag cctttactga cccccagctg aaccaattcc
    27841 cgcgtgaggt gttcacccat ctcccctttg tgattctcac tctgtatcat gacaagttta
    27901 aagacctgcc ggtggcgttc gcgccttcaa aagtccaggc gaccctgggc gaggtagtga
    27961 gccaggcggg tttttcacag gtgcgcattg ctgaatcaga aaaattcgcc cacgtgacct
    28021 tttttttgaa cggcggcaac aaccagccat acccacaaga agaagatatc cgtatacctt
    28081 ctttgaaagg cgttcctttt gatcaatacc cggaattaag cctggagaag gttgcacagg
    28141 ctgtcataga agccattcac agggggacag gcctggtggt gaccaatttt gcgaatgggg
    28201 atgtcatcgg gcacacttcc aataaagcag cgaagatcgc ctgcgcgcag tttgtagacc
    28261 ggcacctgcg cgaagtggta gaaaaggcta acgcggcggg ttatgtggtc atcatcaccg
    28321 cggaccacgg caacctggaa gtgttggaaa acgcggacgg ttcagcgcat gtttctcaca
    28381 ccaccaaccc ggtctcttgt ctgatcatcg atccggcagc cgcggagacg gtcaccccgc
    28441 gtgagggcaa gctggcggac atcgctccga cagtgttgag catcctgcgg gtaaccccgc
    28501 cggcgcaaat gacaggaaag aacctggcgc cggagcatga ctttggggtt gaccgtaaag
    28561 tcttgctggt catcctggac ggttggggaa tcagcggcgt tcctgagaac gatccaattg
    28621 ctatggcgga gacgcctttc tgggatagcc tgctggcgcg ttaccagcat tcgctcctgg
    28681 aggcctcagg tgaagccgtg gggctgaaag ccggcaaagc aggaaactca gaagctggac
    28741 acatcaacat tggcgccggc agggtcgttt tgcaggatga tgtgcgcctg gatagggcga
    28801 tgcaggatgg ctctttctac accaacgagg tcatctgtga cgtcattgaa ggcgtaaaag
    28861 cgcgcgggaa gaacctccac ctcatcgggc ttttgactga aaaaagctcg cacggctcca
    28921 tggaataccc cctggcggtt ttgaaaatgg ccaaagacgc cggcttgcag catgttttcc
    28981 tgcatatgat attggatgga cgcagcactg agcccggaag cgcgcccgcg cttttgaaga
    29041 aactggaaga tttaatgaat gaaatcggcg tcgggcagat cgtcacgggt attggacgcg
    29101 gtatcgccct cgaccgcgac ggtaattaca ataagacgca aatcgcctac gacgcgctgg
    29161 tctatggaaa aggcagggga gccggtaaat gaactacgaa ttcctcaaga aactgatcca
    29221 accggcggat accaaaatcg ttatgctcat catggatggg ctaggagggc tacctaaaga
    29281 acccggcggt aaaactgaac tggaaacagc tcatacccct catatggatg ccctggctgc
    29341 tcggtcggca ttgggctttt ccagccctgc gggggcgggc atcgcagtgg gcagcgggcc
    29401 tgggcacctg gccatcttcg gttttgaccc gatagagcac gagattggcc gcggcgcgct
    29461 ggaagcctta ggggttgata ttgacctggg ggcggaagat atcgctgccc gcggcaactt
    29521 ctgcaaggtg gacgccaatg gcatactaac agaccgaaga gcggggcggc tcgcaacccc
    29581 tgaagccttc aggctggcaa agatgctaaa cagcatcgcc atcgacggcg ttcagataca
    29641 ggttgagcca atcaaagaac atcgtttcgc gctggtcatg cggggcgagg gtttggcggc
    29701 gaagatttca gacaccgacc cgttggtgga gggcgttgct cccaggcaag cggttgcgct
    29761 ggaccaggcc tctgaaaaag ccgcgcggtt ggtcaatgcg tacatccaaa aagcgcaaga
    29821 aatcctccgt tctgaagaac cggcaaatat ggtgctttta cgcgggtttg acaaactgcc
    29881 cgtgctccca aagtacgggg agcttttcaa gttgtgcgcc gcttctatag ccgtgaacgg
    29941 catgtataaa ggcgtttcca ggctggtagg catgcaggta ttaccggtgg a
//
LOCUS       DAOSPM010000009        29838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1561_length_29838_cov_16.316959, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000009 DAOSPM010000000
VERSION     DAOSPM010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 29838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29838
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1561_length_29838_cov_16.316959"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1889
                     /locus_tag="PLW25_01830"
     CDS             222..1889
                     /locus_tag="PLW25_01830"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOS53260.1"
                     /translation="MNKQDKKDSHRKGALKIIPLGGLGEVGANMMVYEYDGQILVVDT
                     GLMFPENDMLGIDYIIPDISYLVQRRKQVKGIIITHGHEDHTGAIRHVLEELQAPIYA
                     TPLTRGLLEVKLARNGGASKAQIQTVNAGENRKIGPFSVDFFHVCHSIPDGVGLGIEC
                     PAGLVVHTGDYKFDHTPVDNWPTDYAKLAEFQRRGVLVLLADSTNSERQGWTPSERVI
                     DDAFDKVFREADGRILIATFASLISRMQQVGNAALKHNRKTAFAGLSMVDNAKIAREL
                     GYLQLPDNLIVSLDEALHMKDSDVVIMCTGSQGEPTSILGRLSRGTHRVFDVKNGDTI
                     VLSSHPIPGNEENVYGTINRLLERGANVIYEAIAPVHVSGHASQEEMKLLLHLTQPEY
                     FIPIHGELRQLKQHARLARQIGIPEENIAVILNGQSVELSHGKMRMAEKVPASYVYVD
                     GSGVGDVSDAVMHDRELLSEEGLMIVNLTMDKYTDRPKKEPDFITRGFIAQQDGNELM
                     TGLKARVMSVADRANGHLQQDVEQAVSNYLYRETRRKPMVLVNIIKS"
     gene            complement(1927..2583)
                     /locus_tag="PLW25_01835"
     CDS             complement(1927..2583)
                     /locus_tag="PLW25_01835"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOS53261.1"
                     /translation="MPPDFAHEREQMVRHQIEKRGITNSGVLNAMRTVPRHLFVPLEM
                     REQAYADYPLPIGSDQTISQPYIVAFMTALLNLTGSEKVLEIGTGSGYQAAVLSLLAR
                     EVITVEYLPALARSAGDTLRDLGYLNVTVHQGDGSLGWVDQAPYDAIMVTASAPKAPE
                     PLLKQLNPEGRMVLPVGGRGVQWLELWQQENGIWLPEVSLPVAFVPLRGAFGWQDDHE
                     "
     gene            complement(2573..3652)
                     /gene="mutY"
                     /locus_tag="PLW25_01840"
     CDS             complement(2573..3652)
                     /gene="mutY"
                     /locus_tag="PLW25_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOS53262.1"
                     /translation="MKDELARLLLNWYRQNARSLPWRGHSDPYAIWVSEIMLQQTRVD
                     TVLPYYLRWMRDFPDISTLANASEHAVLLVWEGLGYYNRARCMRRAAQQMMQLHDGKI
                     PADYEQLLKLPGIGPYTAAAIASMAFGLDRAVVDGNVKRVLARILPYTLAVNTPTAES
                     ALRHAAQELLPEGNAGDFNQALMELGALVCLPRKPLCDRCPLPSICAAFQQGAQAELP
                     LMKEKKPVPHLVVTAAILRQDDRVLIAKRPARGLLGGLWEFPGGKVKENETHADALAR
                     ELMEELGVSTQVGNRFGTYRHAYTHFSVTLHAYEIISQKNTAKPLHHAELRWVALAEL
                     ESYPMGKIDRMISRDLVQAELANAA"
     gene            complement(3661..3957)
                     /locus_tag="PLW25_01845"
     CDS             complement(3661..3957)
                     /locus_tag="PLW25_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53263.1"
                     /translation="MKLIMSWDITPEHEQDYFEFVIRDYIPGVQRMGCELTDAWATVY
                     GNKPQITVSVIIGSPRKARQLLFSEDWLSLNNRLQDFIQNYTMKLVYARNRFQL"
     gene            complement(3978..4643)
                     /locus_tag="PLW25_01850"
     CDS             complement(3978..4643)
                     /locus_tag="PLW25_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS53264.1"
                     /translation="MEITWYGHSCFRLTERSMATVVTDPYDCKQVGYEPLKVKGDVVT
                     VSHNTPGHNLISAVKGYSHLIDGPGEFEIGGVFITGIQTNGHSRKAENEPRNTMYLFD
                     YNGINVLHLGDLNRVPSQAEVEAIGPVHVALVPVGAGGSLTAIKATEVISLLEPNIVI
                     PMHYATPHTASTPQKLEPLSRFLKEMGLSNVEPVPTLKVTGISSLPDETRVVILQYQM
                     NNS"
     gene            4642..4923
                     /locus_tag="PLW25_01855"
     CDS             4642..4923
                     /locus_tag="PLW25_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53265.1"
                     /translation="MQPPQTDYTAKARRRIAIEEISSGANAHSLDGNPGLSSMRTADA
                     GKQLFYLNGASASISFLGANTCFIWFYEIQLPVLTSARFCGDLLIAFFT"
     gene            complement(4916..6013)
                     /locus_tag="PLW25_01860"
     CDS             complement(4916..6013)
                     /locus_tag="PLW25_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53266.1"
                     /translation="MTSVYTIQNPEAFTSPPATPTTGTGCTPSVRGLNPAKPGDTISP
                     GASYADAAGKETSVIQDRWFFNGQESSSMIWDGKPVTVELQWTCLDHSGHSKTFTIPA
                     YQGEAAAPLTQPQTGQPAETSQEAQPKRPKTVAPWGIAAIISSIVGLVGAASVGIGYA
                     LKKKPAAPAPPAPPAQSPPPNIPVQYPPIPDPAGFSKIGAPTRYTPPATAGRQFTADV
                     PVQNTPPQVEPSSHQPAGMTPAQREELLSIRGQMQIEAANLKNKYYDNKEKIDRLKGI
                     LKNNMLKFITKKGFDVQEWMLEGPTDVVNKIIVDPIMEKAFQKHDTSQDGKIIVDMYN
                     RIKQMENEMHELSKEFEYLQNEMNKIDQKLK"
     gene            complement(6080..6466)
                     /locus_tag="PLW25_01865"
     CDS             complement(6080..6466)
                     /locus_tag="PLW25_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53267.1"
                     /translation="MESRIQRTLLSFFFASFFFMFYFLMYLPNDVEAQGEVEPQRVIA
                     TLYDSDCFPNGVENRIEFFNVGSQGGEAYSKAVYVYAITHIGNHPDGGLACFVDGYAE
                     LIGTFSGGPNGTITFPKGNYQEITTC"
     gene            complement(6680..8392)
                     /locus_tag="PLW25_01870"
     CDS             complement(6680..8392)
                     /locus_tag="PLW25_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53268.1"
                     /translation="MERTVPKSATDEIDLYLRTIYSLLRSTTEVQVRTLEEVHAGMNS
                     SLHPDARKVSLDLSALIYSSLRLPSCIMRTRSIVLGQSVQLFSRHGYPEVENWLPVTA
                     RARRRSSYFDGNSTLAVFIASRSDIEDIVPTLTAFQIEWNKMHQFLQGWNPPSWEETS
                     LEGSPSFVELSHVLGTPLEDLQRLVSVWGSDFSSNLETIASRRCSFGIRLLSGSLSEY
                     WRATRLWWQNIERSAPLFSERPVYLISSNTHSIPNLLSGYALTRKEQILTFLQQQGNQ
                     ELLAEWHEIQERGNCASTENFLYYALKKYQQTAQGAAENAQMAREEQRHGITRVPPAH
                     SFDIEAQVVEINRLDPQHFDPRLLSDGENTVFLQKSNALIVNIDYPLGLGAYNVLTKI
                     AEHISEILGVYIMGKAATLNGVRGDIMIPGVVQDEHSQNTYLFQNVFTSPDVAPWLVF
                     GTVLDNQKALTVLGTFLQNASLMDVMYREGYTDIEMEAGPYLSAVYEMTRPKRHPVNE
                     IVNLQGVNFDIGILHYASDTPLSKGKNLGAGTLSYRGMDSTYAASVAILRRIFQQEKK
                     RLLG"
     gene            complement(8516..9298)
                     /locus_tag="PLW25_01875"
     CDS             complement(8516..9298)
                     /locus_tag="PLW25_01875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOS53269.1"
                     /translation="MNFIEGNLGILPASYTTQAVFFDLGFTLIHFQGDYPSVLLDSYY
                     SLARALKREGYLFEVDEFVRRFKNALADYYQSRDIDLIERPVETYLTRVLNTFGHIEP
                     PESVTRNALAEMYQTTEAYWQLMPDTLATLRQLQRTGYRLGLISNAANADNVNRLIDR
                     CGLRGFFEVLLISAVEKIRKPDRRIYARALNLLHLTPDRAVMVGDTLTADIIGAQNAG
                     LRAVWVTSRASHPENLRARPHVNPDAVIDQLSELPYALQQFH"
     gene            complement(9338..10363)
                     /locus_tag="PLW25_01880"
     CDS             complement(9338..10363)
                     /locus_tag="PLW25_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOS53270.1"
                     /translation="MASAQNIEVLTTVPFPEAVMGQLRELSPRIKITCIPASKIDEIP
                     REVWQRSEVLYTDILLPDLNLSPNLRWIQFHYAGIDFLQGSELLKHKDLKLTSMSGAS
                     AIQEGEYILMMLLALGHHMRGLMKNQEKAEWPDDRWEKFSPRELNGSTVGLVGYGSIG
                     REVARLLRPFDVTVLAVKRDVMHPQDNGYVMDGHGDPEGDYFNRLYPIEALKSMLREC
                     DFVVVTLPLTPQTRGMIGEEEFNAMKPGAYLVHISRGGIVDEEALLRALNEKRLAGAA
                     VDVFSTEPLPADHPLWKAPRLIITPHVSGFSQAYKERAGRMFAANLKRYLRGEPLLNE
                     YLSDRHY"
     gene            complement(10374..11150)
                     /locus_tag="PLW25_01885"
     CDS             complement(10374..11150)
                     /locus_tag="PLW25_01885"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOS53271.1"
                     /translation="MSDLTYTASELMCINAARLLRDGDVVFVGVGLPNLACNLARRTH
                     APNLQLIYEAGVIGARPSRLPLSIGDPTLVTGAVSVCGMFDIFSQYLQRGNIDVGFLG
                     GAQIDRFGNINATIIGGDYQHPKVRLPGSGGSQEIAGWANRCYIITPHQKRRFPEKVD
                     FITSAGFLNGRAQRELTGARGGGPLVVVTNLGLLEPDANGELVLSALHPGCTLEQVRE
                     NTGWQVRTSGQVSTTEPPLPSELQLLREELDPGGIYLKGS"
     gene            complement(11168..12070)
                     /locus_tag="PLW25_01890"
     CDS             complement(11168..12070)
                     /locus_tag="PLW25_01890"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOS53272.1"
                     /translation="MSKICSLAEAISSYVHDGDTVFCAGFTHLIPFAAGHEIIRQGKK
                     NLTLARATPDLIYDQMVAAGCAKKLIFSYIGNPGVGSLRIVREELEAGRLEWEEYSHF
                     GMISRLQAGAAGLPFMPMNQTAAPDLERSNPNIRRVKDPYTGQDVVTVPALNPDVGII
                     HVQRADEHGNAQVWGILGEQKEAAFASRDVIITAEEIVSEDVIRSDPNRTLIPGLIVK
                     AVCQVPHCAHPSYTQGYYDRDNVFYLNWDKISEDRATVNAWLDEWVHGVQGAEEYWQK
                     LGSETHARLAVKPRYAAPVNYGDY"
     gene            complement(12079..13662)
                     /locus_tag="PLW25_01895"
     CDS             complement(12079..13662)
                     /locus_tag="PLW25_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOS53273.1"
                     /translation="MAADQVFKLTYATMFNPPEELHTRYDQALAKVKSELGRDHGMLI
                     DGKEVFAEEKFDNRSPANTDVLLGTFQKGNAEHAKAALASARKAARGWAHTPWQERVA
                     LLRKAAAIMDRRTFEMGVVLSLEVGKNRMEGLGDVAETADLIRYACDRMEASDGFVAP
                     MGRDPLSGYESRNVSVLKPYGVWLVISPFNFPGALTGGPSGAALVAGNTLVMKPATDT
                     PWTARLIAECFQEAGLPEGVFNYVTGPGSTLGQALIESPEVDGITFTGSYDVGMKIFR
                     DFAQGRYVRPAILELGGKNPAIVTKNADLEAAAIGIVRSAFGLQGQKCSANSRVLIHS
                     SVYDQLVARLVELTGKLTIGDPTERANYLGPVVNKGSYRDYQGFVGELAAGGKILTGG
                     KVLTDGDLAKGYFCAPTIAEAPYTHRLWKYEMFVPITTVGKFDDLETAMKIANDVEYG
                     LTAGFYGTEEEAAWFFDNIEAGVTYANRPMGSTTGAWPGFQPFGGWKGSGSSGKNAGG
                     LHYLQLYMHEQIQNLVRKV"
     gene            13909..14856
                     /locus_tag="PLW25_01900"
     CDS             13909..14856
                     /locus_tag="PLW25_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOS53274.1"
                     /translation="MIPAAQPVKTAFVVINPVAGIVNAALLQRNIESRFKHKGWQTRF
                     HLTAAGEDLKTIVQQQILSGVDLVVAAGGDGTVAAVAAGMVHAPVPLGIIPTGTWNAI
                     ARNLMIPFSSTRAVNLMTGRHRIKQLDLMAIGETVHAMNLGIGFSSMMIENTARAEKR
                     KFGSLAYFSQGFKQIFGLQQRRYMIEVDGKRYRGRAAEIFVANYGMVGLNVIEASLDI
                     KPDDGKVDVLILRARNLFDLPGMFWQMFVQRKRRTPKYRQLSAAESLFIRTNPPAAVQ
                     ADGELIGKTPVTVQVLPRCVKVIVPLEPPLSFPLQPRRV"
     gene            complement(14838..15569)
                     /locus_tag="PLW25_01905"
     CDS             complement(14838..15569)
                     /locus_tag="PLW25_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53275.1"
                     /translation="MPAADRFEILLLIARPAAGKSEIIDYLKKTTLRQRLQRFHIGKL
                     EEIDDFPMLWTWFEEDALLERMGYPRLHTDSAGYFLGNHLWNLLIERICLEYQKRLRD
                     VPGLAREYTTLIEFSRGSEHGGYRSAFCHLSPDMLERMAVLYVDVSWEESLRKNRLRF
                     NPEKPDSILEHGIPDDKLERLYRQSDWEELSAADPEYLEIQGRRVPYAVFDNSDDVTT
                     ARGTTLGERLREVTFLLWERYTRRG"
     gene            15687..17126
                     /locus_tag="PLW25_01910"
     CDS             15687..17126
                     /locus_tag="PLW25_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS53276.1"
                     /translation="MIESETAHRNKWLILLAIGMGVFLATIDGGIVNIGLNTLVKDFG
                     TPLAVTEWVVLAYMLTISSLMLSIGRLGDMIGKKYLYIAGLAIFTVGSVLCGMSETIY
                     WLIAFRVLQAVGASITMALGTAMITEAFPDHQRGKALGIMGTLVSIGIIAGPTIGGLI
                     LEHLTWHWLFFVNLPVGILGVLMVWHYVPLDRPGKKQSFDFLGAGLLFITLSSLLLAL
                     SRIQSAGLTDPLIPGLLFLFLIGLIVFIRVEKRSAEPMIDLSMFRNRMFSVNLITGFL
                     TFVVGAGTMILFPLYLQNVLGFGPQQSGLMMAVTPLTVAIVAPFSGALSDRVSSRVLT
                     SIGLGVMLVGHSLISTLSTDTSVTGYLLRFIPLGIGIGLFQAPNNNAIMGSAARERSG
                     VASSLLSLTRTVGQTTGIALLGAFWERRATTLAGDDALHSALAAPLKAQVGGLQQTMY
                     LVIAVIIAALGLSLWALAAWLRERSPQNA"
     gene            17249..18343
                     /locus_tag="PLW25_01915"
     CDS             17249..18343
                     /locus_tag="PLW25_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53277.1"
                     /translation="MLASFMLVSCSLPEVTPPAAGETAPVVSTPSEQTVPLPAQVITA
                     TPAQTRLIKASEVGRLEISQRVAVSNIQDISWSPDSNTLALVTQNTDAAQNQVFSVTL
                     LNAKDLSTQAVYSATGNRVTSVAADGKTAAVINQDQMSFSLVDTSVKDGASRSRVTDF
                     LIGNVTISPDMRYVAVTKAEFWEVILYDFNTLEEVRFLSGFSTAAPVFDAGFNHSPQW
                     LVWHARATLQLQEVETGMLSNLYSHEDFVTAYTLSPDGMILASASFRSTNDASVPVVI
                     LWDTLNGTELKVLEISQPVNALQFSPDGKLLAVAAGSDLQIWDAAGGNLLTTLTGHSD
                     FILQLAFSPDQHTIASAGQDKQLYLWQILE"
     gene            18445..>18628
                     /locus_tag="PLW25_01920"
     CDS             18445..>18628
                     /locus_tag="PLW25_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS53278.1"
                     /translation="MPLLTAANLSKSYGALDIFPEISFSIPHRARIGLVGANGVGKTT
                     LLRVLMGEEEPSSGTIH"
     assembly_gap    18629..18728
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18729..20507
                     /locus_tag="PLW25_01925"
     CDS             <18729..20507
                     /locus_tag="PLW25_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOS53279.1"
                     /translation="TLLRVLMGEEEPSSGTIHKARGIRIGYLPQEAALDSSRTLWGEC
                     MTVFEPLLALQQELHAMEEALSANPDSTDLMQVYGERQREFEHLGGYEFEARTRITLS
                     GLGFKRADEKRPVRQLSGGQRTRVLLAKLLLSEPELLLLDEPTNHLDISAVEWLEGFF
                     KEWQGAVLIVSHDRYFLDQTTTTIWEMTPALEEYRGNYSAYLAQREERYQRRLQEYQA
                     QQEFIEKEEEYIRRNIAGQNTRQAQGRRTRLERLLSEARLTPPLQRRTLRMQLVSSGR
                     SGDLVLRTRSLSVGYADEGKPLFEVDDLTLVRGECAAIIGPNGAGKTTFLKTILEQIP
                     PYAGETVLGASLKVGYFAQAHEGLHPDWTLMDEIQAMMPKWLPGEIRDYLAKFLFTGE
                     DVFKTVHLLSGGERGRLALACLALQGANLLLLDEPTNHLDLPSQEILQNILGQFQGTI
                     LLVSHDRYLIDALATQVWEVLPEQRKLAVFEGSYSEYRALKQANLMIPDGRPQQKVGL
                     TRAKKPAVGKSKDQLQRMKARLRALEEEIARLEIDARGVEQRLSDRGVDPGEVQQLAS
                     EYARLQAQLEAKIELWSDLGDELSEP"
     gene            complement(20555..21376)
                     /locus_tag="PLW25_01930"
     CDS             complement(20555..21376)
                     /locus_tag="PLW25_01930"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOS53280.1"
                     /translation="MPDLTIYTNLARKILTDFIHTEITRAGFSRAVLGLSGGLDSALA
                     CYLAAEALQPENVLAVRMPYATSSADSLAHAQLVIDALGVRTLTIPITDMVEPLFNRY
                     PDMGNRRRGNVMARQRMIILFDQSEVFKGLVLGTSNKTEILLGYTTMHGDAACALNPL
                     GDLYKTQLRQLAAAVGVPEPLLQKPPSADLWAGQTDEDELGFTYAAVDQLLYLLVDQR
                     YTPQECVDSGFEVKFVHAVIERVRKNQFKRVMPPIAKLSGRTVGYDFLYLRDWGT"
     gene            complement(21397..22275)
                     /locus_tag="PLW25_01935"
     CDS             complement(21397..22275)
                     /locus_tag="PLW25_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOS53281.1"
                     /translation="MKLNVAMAQINTVLGNVEQNLEKHLDLIEQARRAGADLITFPEL
                     SLTGYALQDLTPTVACRPLPDDPVFNPLLVASQDIDIQVGFVEEDERNLFYIATAFLS
                     GGAVRHIHHKVYLPTYGFFDEGRFFARGESVRAFDTRFGRVGMLICEDFWHASSPYML
                     WLDGADLFLFTSASPGRGITPTPHNTAPGRTESARWVNDIIRAYAGMFTTFVVHTNRA
                     GFEDGIHFWGGSSAFSPDGEPLVNGVEHAEALVLAELDLSQLRRTRTRLPLLRDERTS
                     LVQREITRIITRNADG"
     gene            complement(22292..23026)
                     /locus_tag="PLW25_01940"
     CDS             complement(22292..23026)
                     /locus_tag="PLW25_01940"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOS53282.1"
                     /translation="MLTLRLFSILAGFGAAIGLWRVYRSIPAGVESRQWICAVFALLG
                     VLTGARVGYVLAHPFYYSLHPEQAGQFWLGGFNAFGAFAGAVFFALLGSAALRLNVLS
                     ALDSTSRMLLPTAALIWLGLWYEGVAYGQTLPSGAFLSYPALDETGMLSNRFPLQLVA
                     ALSLLLLLGLIEHITQFKRPGFRFSLVSLVFFTHTLVFSLFRADVVFPIRGIRSDGVL
                     SLIFAVAFALLSVLLLLTSDQKKRIK"
     gene            complement(23076..23330)
                     /locus_tag="PLW25_01945"
     CDS             complement(23076..23330)
                     /locus_tag="PLW25_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOS53283.1"
                     /translation="MMSDNCCSYSELELLRGLIEQLSAYINTYHGGEVEFVSFDGKVV
                     KVRMGGACVGCPHSPATIKGWVEGTVRQFFPEITGVEAIE"
     gene            complement(23330..23815)
                     /locus_tag="PLW25_01950"
     CDS             complement(23330..23815)
                     /locus_tag="PLW25_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOS53284.1"
                     /translation="MLNGSPLPVQIFEGFSLDEQDELRSIIEVFTYPAGETILRQSQQ
                     AVHLFILVTGEVEITHVPYDGPELTIGKLTSGGVFGWSSILGRKTYSSSVFATTDSRV
                     YRIAAYKLQRFCEQHQESGMVLLEKIAMSVAQQPAKAHDQIMHMLRAVIACPEGKNPE
                     E"
     gene            complement(24188..25000)
                     /locus_tag="PLW25_01955"
     CDS             complement(24188..25000)
                     /locus_tag="PLW25_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53285.1"
                     /translation="MLEVNALSAGYYHELVLEDISLQLPQGSVLALIGPNGSGKTTFI
                     RAISGVLPLRAGSVKVAGQDIYSLSPQQRARIIAVVPQARSIPPAFSAEEVVALGRTP
                     HLNWLGKVNGKDMLIIEEALEKMDLLALRSRAVSELSGGEQQRLFLARALAQESPLLL
                     LDEPTTHLDLQYQVSLMQRIHQLAHPTKEELVKGMQPKTVVVAIHDLNLVSRFADQVG
                     LLVGGKLAALGKPEEVLNAGLLSVAYNLPLEVLKEHGYTLVIPAHTPARNNS"
     gene            complement(25006..>25415)
                     /locus_tag="PLW25_01960"
     CDS             complement(25006..>25415)
                     /locus_tag="PLW25_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOS53286.1"
                     /translation="YMLIALAGLVLIGHPLNVLQFGEEQALQLGIRVQRARWITIALA
                     TLSTAAAVAFAGIIGFVGLVVPHIIRLLWGTDYRRLIPLSMLGGASLLLLTDVVARSL
                     IAPQEIPVGIVTALLGAPFFLWVLKLAKAQNYW"
     assembly_gap    25416..25515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25516..26195)
                     /locus_tag="PLW25_01965"
     CDS             complement(<25516..26195)
                     /locus_tag="PLW25_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOS53287.1"
                     /translation="MRKKNTRSPFLVLALALAASALLSVAVGSVFIPLPTLWDVVTSA
                     LRGATSTLDVNGVYLKIISDLRLPRMLMLTMVGAALAGSGAAYQGLFRNPLADPFLIG
                     VSSGAGLGAVLAMLARSPYTRGGLLSVPLAAFCGALLTVLIVTLLARVGKTVPTTNLI
                     LAGVAVSSFTSAVTSALMIHSTGELRRALVWLMGGAIMTGWSPILAVLPYMLIALAGL
                     VLIGHPLNV"
     gene            complement(26195..27118)
                     /locus_tag="PLW25_01970"
     CDS             complement(26195..27118)
                     /locus_tag="PLW25_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOS53288.1"
                     /translation="MKHARITRIAVFLLLLMLAACSAIPAAKDGGKIQLQDGLGREVL
                     LEKPAARIISLSPSVTEILFAVGAGSQVVGRDTFSNYPEEVTSIQDVGGSMGSYSLET
                     IASLNPDLVIAAEINTPEQVSALEGLGVKVYYLSNPTDLEGIYGLLQTVGALSGHTKE
                     AAALTSSLKERVQLVQQAIKKAETKPVVFYELDGTDPAKPWTPGPGSFLDLLINEAGG
                     RNAAAAMQSQWAQISIEELLVQNPDIILLGDSIYGMTPEQVAARPGWDGLRAVQEGRV
                     YPFNDDLVSRPGPRLVDGLEELARLIHPELY"
     regulatory      complement(27223..27362)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            27485..28015
                     /locus_tag="PLW25_01975"
     CDS             27485..28015
                     /locus_tag="PLW25_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOS53289.1"
                     /translation="MTRVPYEIRVGELLKEKQKKLVLAESCTGGLVGNLITNIAGSSD
                     YYLGSVTAYAYEAKEALLGVRHETLLQYGAVSRQTVLEMAAGARRSLAGGIPLDSIIG
                     VSISGIAGPGGGLPDKPVGTVWFGMSTADGDWAWRCMFKGNRVQNKRSAARTALKLVI
                     AYLAGSLPPETRSQRH"
     gene            28025..29236
                     /locus_tag="PLW25_01980"
     CDS             28025..29236
                     /locus_tag="PLW25_01980"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS53290.1"
                     /translation="MAKRLIRILILTADAGFGHRSAANAVAKALKLKYQSAVEVRVEN
                     PLDLESAPFFLREAQTDYDRWVKQVPELYKLGYQASDAAIPTRLLEDSLAVLLFEAIR
                     DTFSGPLPDVVLTTYPMYQSAIMLLYRLRKIHIPLFTVVTDLSTVHRLWFNRNVDGCL
                     VPNEIVANLAAVNGVPQDKVHITGIPVSPDILYETRPAEKIRAELGWHPELTTILAVG
                     STRTEKLLEAASVINHYGSELQLVVVAGKNEELYQELNQIGWHIPVQLFDFVDKVPLL
                     MKASDLVICKAGGLIVTEALACGLPMVLTDIIPGQETGNAELIQRLQAGVVAETPLMM
                     MEALHHFLMNDQKMLRQYADNARTIGKPAAACDVAEILFEAGKASTPPKASYRAHGFR
                     SAEQPLEGDSE"
     gene            29233..>29838
                     /locus_tag="PLW25_01985"
     CDS             29233..>29838
                     /locus_tag="PLW25_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOS53291.1"
                     /translation="MKENGEVEVAILFSAHSEWQTAQSLFLDTGNENSPFGDYFLTCV
                     AEKRCVFFHGGWGKISAAASTQFVIDRWQPKLLVNLGTCGGFEGHTAVGEVVLVDETM
                     VYDIHERMNDPFEALQFYTTRIDLSFLRRPYPQAVRIGRLVSADEDIDPAKINRLRQE
                     FGAVVADWESAAIAWTAQRNGVRVLILRAVSDLVSESGGELY"
BASE COUNT         6716 a         7892 c         8390 g         6640 t
ORIGIN      
        1 tggattgaag aaccattttg ttcaaacatt gcgtttttgc tctgctcaag cagagatgaa
       61 gtgccacaaa aataaaggaa gtcactcgca aaccctggct tgaacaaccg ccgggatttg
      121 ttttttatga accatatccg cggttttaag gggcgacctt tcgacgcggt tattccaatc
      181 aaaattcctt aaatggaaca ggaaaggtat aaaagtaatt aatgaataag caagataaaa
      241 aagatagcca ccgcaagggt gcattaaaga tcatccccct cggtgggctg ggagaagtcg
      301 gcgccaatat gatggtgtac gaatacgatg ggcagatcct ggtggtcgat accggtttga
      361 tgttcccgga aaacgatatg ctgggaatcg attacatcat cccggatatt tcttacctgg
      421 tgcagcggcg caagcaggtg aagggtatca tcatcaccca tggacatgaa gaccacaccg
      481 gcgccatccg ccatgtgctg gaagaattac aggcgccgat ttatgccact cctctgacgc
      541 gcggcttgtt ggaagtaaaa ctcgcccgca atggaggcgc ttcaaaagcg cagattcaaa
      601 cggtcaacgc gggtgagaac cgaaaaattg gcccgttcag cgtggatttc ttccacgtgt
      661 gtcactctat tcctgatggc gtggggctgg gaatcgagtg cccggctgga ctggtggtgc
      721 acaccggcga ttacaagttt gaccacacgc cggtggacaa ctggcccacc gactacgcca
      781 aactggcgga gtttcagcgc cgcggggtgc tggtgctgct ggcagactcc accaactccg
      841 agcgccaggg atggacccct tcggagcgcg tcatcgatga cgccttcgac aaagttttcc
      901 gcgaagccga cgggcggatt ctcattgcca cgtttgcctc tctcatctcg agaatgcagc
      961 aggtgggcaa cgccgcgctt aagcacaacc gaaaaacggc tttcgcgggt ctcagcatgg
     1021 tagataatgc caaaattgcc cgtgaactag gctaccttca actgccagat aacctgattg
     1081 tcagcttgga tgaagccctt catatgaagg acagcgacgt ggtgatcatg tgcaccggtt
     1141 cgcagggcga gccaacttct attcttgggc gtctttcgcg tgggacgcac cgggttttcg
     1201 atgtgaagaa tggtgatacc atcgtgcttt cctctcaccc catcccgggc aatgaagaaa
     1261 acgtgtacgg cacgatcaac cgcctgctgg agcgcggcgc taacgtgatt tatgaagcca
     1321 ttgcgccggt gcatgtttct ggtcacgcca gccaggaaga aatgaaattg ctgctgcacc
     1381 tgacacagcc cgagtatttt attcccatcc acggcgaact gcgccagctc aagcaacacg
     1441 cccgcctggc caggcaaatc ggcattcccg aggaaaacat cgcggtcatt ctgaatggac
     1501 agagcgtgga actgagccat ggcaagatgc gaatggctga gaaagttccg gcgtcgtacg
     1561 tttatgttga cggatcaggg gttggagacg tgagcgacgc ggtgatgcac gaccgtgagc
     1621 tgctttccga agaaggcttg atgatcgtga acctcactat ggataagtac acagaccgac
     1681 cgaaaaaaga gcctgatttc atcacgcgcg ggttcattgc ccagcaggac ggcaacgaat
     1741 tgatgaccgg gctgaaagcc cgggtgatga gcgtggcgga tagggcgaat ggtcacctgc
     1801 agcaagatgt tgaacaggcg gtttccaatt acctgtaccg tgaaaccagg cgcaagccga
     1861 tggtgttggt aaatattatt aaaagttaag caagtgaaat caaaacgctc ctcaaaggag
     1921 cgttttttat tcgtggtcgt cctgccagcc gaaagcgcca cgcagcggca caaatgccac
     1981 cggcagcgaa acctctggca accagatgcc attctcctgc tgccaaagtt ccagccattg
     2041 cacaccccga ccacctacag gcagcaccat tctgccttca gggttcagct gtttcaacag
     2101 cggttccggc gccttggggg cgctggcggt gaccataatg gcatcatacg gcgcctggtc
     2161 aacccatccc agcgacccgt ctccctggtg gacggtgaca ttcaaatagc cgagatcccg
     2221 caaggtatcg cctgcgctgc gagcaagcgc gggcagatac tcgaccgtga tcacttcgcg
     2281 cgccagcaac gaaagcaccg ccgcctggta acccgaacct gtaccgattt ccagcacttt
     2341 ttcagacccg gtgaggttta acagagcagt cataaaggcc actatatagg gctgggaaat
     2401 ggtctggtcg ctgccgatgg gcagcgggta atcagcgtaa gcctgctcgc gcatctccag
     2461 ggggacgaaa aggtggcggg gcactgtccg catggcgttg agcactccgc tgttggtaat
     2521 gccgcgcttc tcaatctggt ggcgcaccat ctgctcacgt tcgtgggcga agtcaggcgg
     2581 catttgcaag ttccgcttga accaaatctc gcgagatcat acggtctatc ttccccattg
     2641 ggtagctctc cagttcagcc agcgcgaccc agcgcagctc agcgtgatga agcggtttgg
     2701 ctgtattttt ttgagagata atttcataag cgtgcagggt gacgctgaag tgtgtgtaag
     2761 cgtggcggta ggttccaaac ctgttgccaa cctgagtaga aacacccaac tcttccatca
     2821 actcgcgggc gagcgcgtca gcgtgggttt cattctcttt cactttccca ccagggaatt
     2881 cccacaagcc gcccagcagc cccctggcag ggcgtttggc gatgagcacc cggtcatcct
     2941 ggcgcaagat ggcagcggtg acgaccaggt gtgggacggg ctttttttct ttcatgagcg
     3001 gcagctcagc ttgggcaccc tgttggaaag cggcgcaaat ggacggaagc ggacagcggt
     3061 cacagagcgg cttacgcggc aggcagacga gcgcgccaag ctccataagc gcctggttga
     3121 aatcgccagc gttcccttct ggcagcagct cctgggcggc gtgccgcagc gcgctctccg
     3181 ccgtgggggt gttcaccgcc agggtgtaag gcaaaattcg agccagcacg cgcttgacgt
     3241 tgccatccac caccgcgcgg tccaaaccga atgccatgga ggcaatggcg gcggcagtgt
     3301 acggaccgat ccccggcagc ttcagcagtt gttcataatc ggcgggaatt tttccatcat
     3361 gcagctgcat catttgctgc gcggcgcgcc gcatgcaccg ggcgcgattg tagtagccca
     3421 gaccttccca gacgagtaac accgcgtgtt cactggcgtt cgccagcgtg gaaatatccg
     3481 ggaagtcgcg catccagcgc agatagtaag gcaacactgt atccacccgg gtttgctgca
     3541 acattatttc agatacccag atggcgtaag gatcggaatg cccgcgccag ggaagggagc
     3601 gggcattttg gcggtaccag tttaacagaa gccgggcaag ctcatctttc atggggtgga
     3661 tcacagttga aaacggttgc gcgcgtagac cagcttcatc gtgtaattct gaatgaaatc
     3721 ctgcaggcgg ttgttcaaag aaagccagtc ttcagagaac aacaactggc gggctttacg
     3781 cgggctgcca atgatcacgc tcaccgtgat ctgtggtttg ttgccgtaaa cagtagccca
     3841 ggcatccgta agctcacagc ccatgcgctg cacgccgggg atgtaatcac ggatgacaaa
     3901 ctcaaaataa tcctgttcgt gctcaggcgt gatgtcccac gacatgatta atttcacaag
     3961 attctgttct tccacggtca cgagttgttc atctggtatt gcagtatcac cacccgtgtc
     4021 tcatcgggca gcgagctgat cccggttact tttaaggttg gaacaggctc aacattggag
     4081 agccccattt ccttgagaaa cctggagaga ggttcgagtt tttgcggggt tgaggcggta
     4141 tgcggggtgg cgtaatgcat agggatgacg atattgggct ccaacaggct gatcacctca
     4201 gtggctttga tggcggtcag gctgcccccg gctcccaccg gtaccagcgc cacatgcacc
     4261 gggccaatgg cttctacctc cgcctggctg ggtacacggt tgaggtcgcc caggtgcaaa
     4321 acattgatgc cgttgtaatc aaagaggtac atggtattgc gcggctcatt ttcagccttg
     4381 cgggagtgcc cattggtttg aatgccggtg atgaagaccc cgccaatttc aaactcaccc
     4441 ggtccgtcaa tgaggtgaga ataacctttc accgcgctga tgagattgtg accgggcgta
     4501 ttatggctga cggtaacgac atcccctttg acctttaagg gctcataacc gacctgctta
     4561 caatcgtaag ggtcggtgac cactgttgcc atactgcgtt ctgttaagcg aaaacaggaa
     4621 tgtccgtacc aggtgatttc catgcaacct ccacagaccg attataccgc aaaggctcgc
     4681 aggaggattg ccatcgagga gataagctca ggagccaacg cgcactcgct ggatggtaac
     4741 ccggggttga gttccatgcg cacggcagat gcaggaaaac agctttttta tctcaatggt
     4801 gcatcagcat ccatttcgtt cttgggggcg aacacctgtt tcatttggtt ctacgaaatt
     4861 caactacccg tattgacgag cgcacggttc tgcggtgacc ttcttattgc tttttttact
     4921 taagtttttg atcgatcttg ttcatctcgt tttgcagata ctcgaactct ttcgatagtt
     4981 cgtgcatttc gttttccatc tgtttgatgc ggttgtacat atccacgatg atctttccgt
     5041 cctgtgaggt gtcgtgcttc tgaaacgctt tttccataat ggggtccacg atgatcttgt
     5101 tcaccacatc ggttgggcct tccagcatcc attcctgaac atcaaagcct tttttagtaa
     5161 tgaatttgag catgttgttc ttcaaaattc ctttaagccg gtcgattttt tccttgttgt
     5221 cataatattt atttttcaag ttcgcggctt caatttgcat ctgaccgcgg atgcttaata
     5281 gttcctcccg ctgggcaggc gtcatacctg ccggctgatg tgatgagggc tcaacttgtg
     5341 gtggggtgtt ctgcaccgga acatctgcag taaattgcct gcccgcggtt gccgggggag
     5401 tataccgagt tggagcgcca atcttcgaga accctgccgg atcgggtatt ggaggatatt
     5461 gaactgggat gttcggtgga gggctttgcg cgggtggtgc tggtggagct ggcgcagcag
     5521 gctttttctt gagcgcatag ccaattccaa cactggctgc gcccactagg ccaacaatgc
     5581 tgctgatgat ggccgcgatc ccccagggcg ccacagtttt tggccgtttg ggctgtgctt
     5641 cctgcgaggt ttcagcgggc tggcctgtct gtggctgggt cagcggtgct gcggcttcgc
     5701 cctggtaagc agggatggtg aaggtctttg aatgaccgct gtgatccagg cacgtccact
     5761 gcagttccac tgtcacaggt ttaccgtccc aaatcattga ggagctttcc tgaccgttga
     5821 agaaccaacg atcctgaatg acgctggttt cctttcctgc cgcatccgca taactggctc
     5881 ctggcgagat cgtgtcgccc ggtttggcgg gattcaaacc gcgcacactg ggtgtgcagc
     5941 ccgtgcctgt ggttggggtg gcaggtgggg aagtaaaagc ctcagggttt tggatcgtat
     6001 atactgaggt catttcttgg attccaaaat tgggtaaatc catcacaaac agacactcga
     6061 tcgtttttcc atcgattacc tagcaagtgg taatctcttg ataatttcct ttagggaatg
     6121 tgatcgtgcc attgggtccg ccggaaaagg tgccaattaa ttccgcataa ccatccacaa
     6181 aacaagcaag ccctccatcc gggtggtttc cgatatgggt aatcgcataa acatagacag
     6241 cctttgaata ggcttctcct ccttgagaac ccacattgaa aaattcaatt cgattttcca
     6301 ccccattcgg gaagcaatca ctatcgtaca aggttgcaat gacgcgctga ggttccacct
     6361 cgccctgtgc ttcgacatcg ttgggcagat acatcaaaaa ataaaacata aagaaaaagg
     6421 aagcgaaaaa aaatgagagt agcgtgcgtt ggatgcgaga ttccatgtga acatcctttc
     6481 ttgcacaacc tcattagaaa gttcttaatc cctgtcttta ttttagcacg tatcgtaaac
     6541 agctaacccc tgtgcgcatt cattcaaatt ggagacaaaa gggagacaag ggctgactca
     6601 cctcgttgat aagaatattt ctgcggggac gatagaaggg ctccggattc gggtttttgc
     6661 tgcccagtta tgctgagcat cagcccagca ggcgcttttt ttcctgctgg aaaatacgcc
     6721 tgaggatggc cacagaagcg gcgtaagtgg agtccatgcc gcggtatgaa agagtgcccg
     6781 cgccaaggtt tttccccttg ctcaacggcg tatccgaggc gtaatgcaaa atgccgatat
     6841 caaaattgac gccttgcagg ttgacaattt cattcaccgg gtggcgcttg ggacgggtca
     6901 tctcgtacac cgctgaaaga tatggaccag cctccatttc aatgtcggtg taaccctcgc
     6961 ggtacatcac atccatcaaa ctggcattct gtaagaaagt ccccagtacc gtgagcgcct
     7021 tctgattatc gagcacagtg ccaaaaacca accatggagc cacatcaggg gaggtaaaga
     7081 cattctggaa aaggtaagtg ttctgagaat gctcgtcttg caccacgccg gggatcatta
     7141 tgtcaccgcg cacgccattc agcgtggcgg ctttgcccat aatatacacc ccgaggatct
     7201 ctgagatgtg ttcggcgatt ttggtaagca cattgtaggc gccaagcccc agcgggtaat
     7261 ctatgttgac aatcagcgcg ttgctttttt gcaaaaaaac cgtattttca ccatccgaca
     7321 gcaggcgcgg gtcgaagtgt tgggggtcaa gcctgttgat ctccaccacc tgggcttcga
     7381 tatcgaagga gtgggcggga ggtacgcgcg tgatgccgtg gcgctgttct tcgcgcgcca
     7441 tttgagcgtt ttccgcggcg ccttgtgcgg tctgctggta tttcttcaac gcgtaataga
     7501 gaaagttctc ggttgaagcg cagttgccgc gctcctggat ctcgtgccat tctgccagta
     7561 attcctggtt tccctgttgc tgcaggaagg tcaaaatttg ctctttacgc gtgagcgcgt
     7621 acccactgag caggttggga atactgtggg tgttactgga gatgagatac accgggcgct
     7681 cactgaacaa aggggcgctg cgctcaatat tttgccacca caggcgcgtg gcgcgccagt
     7741 attcgctcag cgaaccgctg agcaaccgga tgccaaaaga acaccgtctg ctggcgatag
     7801 tctccaggtt actggaaaag tcactccccc acactgatac cagccgctgc aggtcttcga
     7861 gaggggtgcc taaaacgtgg ctgagttcca cgaatgatgg gctgccctca aggctggttt
     7921 cttcccagga aggcggattc cagccttgca ggaactggtg catcttattc cactctatct
     7981 ggaaagcggt gagggttggc acaatatctt cgatatcaga acggctggcg atgaacaccg
     8041 ccagggtgct gtttccatca aaataactgc ttcgccgccg cgcccgcgcg gtaaccggca
     8101 gccagttctc cacttcaggg tagccatggc gcgaaaacaa ctgaacgctc tgacccaaca
     8161 cgatagaacg cgttcgcatg atgcaggatg gcaggcgcag gctggaataa ataagggctg
     8221 aaagatcgag agatacttta cgcgcgtctg ggtgcagcga cgaattcatg ccggcgtgta
     8281 cttcttccag cgtgcgcacc tgcacctcgg tggtggagcg cagtaaagag taaatcgttc
     8341 tcaggtacag gtctatttca tctgtggcgc tctttggcac agttctttcc attcttttgt
     8401 tctcctggct gtattgtagc acaggcaaag cggagcaaaa acagtcagca cttcagcgcg
     8461 atagcgggct ttcaccagta acatgcaggc gaacttttgc tatgctgatg cgcagttaat
     8521 ggaattgttg cagcgcgtat ggaagttcac taagctgatc gataaccgcg tctgggttaa
     8581 cgtgggggcg tgcgcgcagg ttctctggat ggctggcgcg ggaagtgacc cataccgcgc
     8641 gtaaaccggc gttctgcgca ccgatgatat cagccgttaa agtgtctcca accatcaccg
     8701 cgcggtcagg ggtcaagtgt aagaggttga gcgccctggc gtaaattcgc ctgtcaggtt
     8761 tacggatttt ttccacggcg gagataagga gtacctcaaa aaaaccgcgc agcccgcagc
     8821 gatcgatcag ccggttgacg ttatcagcgt tagcggcgtt gctgatcaga cccaggcggt
     8881 agcctgttcg ctgcaactgc ctcaatgttg ccagcgtgtc gggcatcaac tgccagtaag
     8941 cctcagtggt ttggtacatt tcagccagcg cattgcgcgt gaccgattcg ggtggttcaa
     9001 tgtgaccgaa ggtgttcaac accctggtaa ggtaggtttc tactggtctt tcgatgaggt
     9061 ctatgtcacg tgattggtaa taatctgcaa gagcgttttt aaaacgcctg acaaactcat
     9121 ctacttcaaa caggtagccc tctcgtttaa gcgcgcgtgc cagggaataa tacgaatcaa
     9181 gcaaaacaga tgggtaatca ccctggaagt gaatcagcgt gaaacccaaa tcaaaaaaaa
     9241 ccgcctgcgt tgtataggag gcggggagaa tgcccaggtt cccctctatg aaattcatca
     9301 ggtgttcacc atctacctga tttaacccat ccaaagctca atagtgacgg tcgctgaggt
     9361 attcattcaa caagggttcc ccccgtaaat agcgcttcag gttggcggcg aacatcctgc
     9421 cggcgcgttc cttgtaagcc tggctgaaac ccgaaacatg cggggtgatg atcaggcgcg
     9481 gcgctttcca gagcgggtgg tcggcaggga gcggctccgt gctgaacaca tccacagccg
     9541 ccccggctag gcgtttttcg tttaacgccc gcaacagcgc ttcttcatcc acgatacctc
     9601 cgcgtgaaat gtgcaccagg taagcgcccg gcttcatggc gttaaattct tcttcgccga
     9661 tcattccgcg ggtttggggt gtgagcggca gggtgacgac cacaaaatcg cattccctga
     9721 gcattgattt gagggcttcg atagggtaaa ggcggttgaa ataatcacct tctgggtctc
     9781 cgtgaccatc catcacgtaa ccgttatcct gcgggtgcat gacatcacgc ttaacggcga
     9841 gcacagtcac gtcaaacggg cgcagcaggc gggctacttc acgcccaatg ctgccataac
     9901 caaccaaccc cacggttgag ccgtttaatt cacgcggtga gaatttttcc cagcggtcat
     9961 cgggccattc ggctttttcc tggtttttca tcagtccccg catatgatga ccaagcgcca
    10021 acaacatcat caggatatac tcgccttcct gaatggctga agcaccgctc atggaggtta
    10081 atttgagatc cttgtgttta agcagttctg acccctgcaa aaagtcaata ccagcgtagt
    10141 gaaactgaat ccacctgaga ttgggagaca ggttgaggtc tggcagcagg atatccgtat
    10201 agagaacttc ggagcgctgc cagacctcgc gcggtatctc gtcgatctta ctggcgggga
    10261 tgcaggtgat tttgatgcgt ggagaaagct cgcgcagttg acccattacc gcttcgggga
    10321 agggcactgt ggtgagaact tcgatgtttt gcgctgacgc catgatgaac tgctcaagaa
    10381 cctttcaggt aaatcccgcc tggatcgagc tcctcgcgca acaactgaag ctcagatggc
    10441 agtggaggtt cagtggtgga tacttgcccg gaggtgcgta cctgccagcc ggtgttctcc
    10501 cgcacctgtt caagggtgca gcccgggtgc agggcgctga gcacaagttc gccgtttgcg
    10561 tccggttcca acaagcccag gttggtcacc accaccagcg gcccgccacc cctggcgccg
    10621 gtcagttcgc gctgcgcgcg cccgttcagg aatcctgctg aggtgataaa atccactttt
    10681 tcgggaaagc ggcgtttttg atgcggcgtg atgatataac accggttggc ccagccggcg
    10741 atttcctgcg atccgccgga acccggcagg cgaaccttgg ggtgttgata atctccgccg
    10801 atgatggtgg cgttgatatt accaaaacga tcaatctgcg cgccgcccag aaagcccacg
    10861 tcgatgttgc cgcgctgcag gtactgggaa aagatatcga acataccgca gacggatacc
    10921 gccccggtta ccagcgtggg gtcaccgatg gaaagaggta accgcgaagg ccgggcgccg
    10981 ataacgccgg cttcgtaaat taactgcagg ttgggggcgt gggtacggcg cgccaggttg
    11041 cacgccaggt tgggcagccc aaccccgaca aagaccacat cgccatctcg cagcaggcgg
    11101 gcggcgttga tgcacatcag ttctgaagct gtataagtga gatcgctcat accatttcca
    11161 ccaggtgcta ataatccccg tagtttacgg gagctgcgta acgaggtttt accgccaggc
    11221 gggcgtgagt ttcgctcccc agcttctgcc agtattcttc cgcaccctgc acgccgtgca
    11281 cccattcatc gagccaggcg ttgaccgtcg cgcggtcttc actgatctta tcccagttga
    11341 gatagaacac attgtcgcgg tcgtaatatc cctgggtgta agagggatgc gcgcagtgcg
    11401 gtacctggca gacggcttta acaatgagac caggaatcag cgtgcggttg gggtctgaac
    11461 ggatgacatc ttcgctcacg atttcttcag cggtgataat gacatcgcgc gaagcgaagg
    11521 cggcttcttt ttgttcacca aggatgcccc aaacctgcgc gttaccgtgt tcgtcagcgc
    11581 gctgcacgtg gatgatgccg acatcagggt tgagggcagg cacggtaacc acatcctggc
    11641 cggtgtaagg gtctttcacg cggcgaatat tcggattgct gcgttccagg tctggagcgg
    11701 cggtctgatt catgggcatg aaaggcaggc cagcggctcc agcctgcaag cgcgagatca
    11761 tgccgaaatg ggaatactct tcccactcca gacgcccggc ttcaagctct tcgcgcacaa
    11821 tgcgcagtga gcctacgccg gggttaccga tgtaagaaaa gattagcttt ttcgcgcagc
    11881 cagctgctac catctgatca tagatcaaat caggggtcgc gcgtgccagg gtaaggttct
    11941 ttttaccctg gcggatgatc tcgtgaccgg cggcgaaggg gatgagatgg gtaaacccgg
    12001 cgcaaaagac cgtgtcgcca tcatgaacat aactggaaat agcctcagcg agcgagcaga
    12061 ttttggacat atcgcttttt acaccttgcg aaccaggttc tggatctgtt cgtgcatata
    12121 gagttgcagg taatgtaaac cgccggcatt cttaccgctg gaacctgagc ctttccagcc
    12181 gccgaaaggt tggaagccag gccaggcccc ggtggtggaa cccattggac ggttggcgta
    12241 agtgaccccg gcttcaatat tatcgaagaa ccacgcggct tcttcttctg taccgtagaa
    12301 cccggccgtc agaccgtact ccacgtcgtt ggcaattttc atggcggttt ccaggtcatc
    12361 gaacttgccg acggtggtga tgggtacgaa catctcgtac ttccacaggc ggtgggtgta
    12421 aggggcttca gcgatggtcg gcgcgcagaa gtagcctttg gcgagatccc catcggtgag
    12481 caccttgccg ccggttagaa ttttgccgcc tgccgccagt tcgccaacaa aaccctggta
    12541 gtcgcggtag ctgcccttgt tgacgacagg acccaggtaa ttggcgcgct cggtggggtc
    12601 gccgatggta agcttgccgg tcaattccac caggcgggcg accagctggt cgtaaaccga
    12661 agaatggatg agcacgcgcg agttggcaga gcatttttgc ccctgcagcc caaaggcgga
    12721 gcgcacgatg ccaatggcgg ctgcttcaag gtcagcgttt ttggtgacga tggcggggtt
    12781 cttgccaccc agctcgagga tggcagggcg cacgtagcga ccctgagcga aatcgcgaaa
    12841 gatcttcatg cccacatcat aagaaccggt gaaggtgatc ccgtccactt cggggctctc
    12901 aatcagcgcc tggccgagcg tggagccagg cccggtgacg tagttaaaca caccttccgg
    12961 gagaccggct tcctggaagc attcagcgat caggcgggcg gtccaggggg tatcggtggc
    13021 gggtttcatc accagggtat tgccggctac cagcgcggcg cctgaagggc cgccggtgag
    13081 cgcgccgggg aagttgaagg ggctgatgac cagccacacc ccgtaaggtt tgagcacgga
    13141 aacattgcgg ctttcatagc cgctgagcgg gtcgcgtccc ataggggcaa caaagccatc
    13201 gctggcttcc atacgatcgc aggcgtaacg gatgagatct gcggtttccg ccacgtctcc
    13261 cagcccttcc atccggttct tgcccacttc aagcgataaa accacaccca tttcaaaagt
    13321 gcgccggtcc atgatggcgg cggctttgcg caacagcgcg acgcgttctt gccatggcgt
    13381 atgcgcccat cccctggcgg ctttgcgcgc agaggcgagc gcggctttgg cgtgttcagc
    13441 gttgcctttt tgaaaggttc ccagcagaac gtcggtgttg gcgggcgagc ggttatcgaa
    13501 cttctcttcg gcaaacacct ctttgccatc gatcaacatt ccgtgatctc gaccgagttc
    13561 tgatttgact ttggcgagcg cctggtcata acgggtgtga agttcctcgg gcgggttgaa
    13621 catggtggcg taagtgagct tgaagacttg atctgcagcc atctgaaaat gcctcctgta
    13681 taaggtcaga gattgcccgt gggttttacg cgggatgttt taagtatagc aaatgagaag
    13741 aaaggcgtca aagtaaaagc gctaactgag cccaaaaggt tatgacttcc ctttcaagat
    13801 ccgcgctgac gaacgctcaa ttgaacacag tacagattca atgaaaacga gtttctcaaa
    13861 cgatggcgat tcttttataa ttatcaaaga tcctggaaaa gagggtgttt gatacctgca
    13921 gcgcaacctg taaagacggc ttttgttgtc attaatccgg tggccggcat tgtcaacgcg
    13981 gcattgctgc aacggaacat tgaaagccgt tttaagcaca aaggctggca aacccgcttt
    14041 catctgacgg cggcgggtga agatttgaag actatcgtgc agcagcaaat tctttcgggc
    14101 gtggacctgg tggtagcggc aggcggagac ggcactgtcg cggcggtggc agcggggatg
    14161 gtgcatgcgc ctgtcccgtt gggtatcatc cccacgggca cctggaatgc catcgcgcgc
    14221 aacctgatga tcccctttag cagcacgcgc gcggttaatt taatgaccgg caggcatcgc
    14281 atcaagcagc ttgatctaat ggcgattggt gaaacggtgc acgctatgaa cctggggatc
    14341 ggattcagct cgatgatgat cgaaaatacc gcccgcgcgg aaaaaaggaa atttggcagc
    14401 cttgcgtatt tttcacaggg gtttaaacaa atttttggat tacaacagcg ccgttatatg
    14461 atcgaagtgg atgggaagcg ctaccgcgga agggcggcgg aaatttttgt ggcgaactat
    14521 ggcatggtgg ggttgaacgt gatcgaagca tcgctggata tcaagccgga tgacggcaag
    14581 gtggatgtgc tcatcttgcg ggcgcgcaac ctgtttgacc tgcctgggat gttctggcag
    14641 atgtttgttc agcgcaagcg ccgaacgccc aagtaccggc agctctccgc ggctgaatcg
    14701 ctgtttattc gcactaaccc accagcggct gtacaggcgg atggcgagtt gattggtaaa
    14761 accccggtca cggtgcaggt gttaccccgt tgcgtgaagg tgattgtgcc gcttgaacct
    14821 cccctctcat ttccccttca accgcgacgg gtataacgct cccacaacaa aaaggtgacc
    14881 tccctgagcc gttcaccgag cgttgttcca cgcgctgtgg tcacgtcatc cgaattatcg
    14941 aaaaccgcgt agggcacacg ccgaccctga atctccaggt attccgggtc agcggcgctt
    15001 agctcttccc aatctgattg gcggtagagc ctttccaatt tatcatcggg gataccgtgc
    15061 tcaaggatgc tgtctggttt ttctgggttg aagcgcaacc tgttcttgcg cagtgattct
    15121 tcccaactta cgtcaacata cagcaccgcc atacgctcca gcatatccgg ggagaggtgg
    15181 cagaaggcag agcggtatcc accgtgttca ctgccgcggg agaattcaat aagcgtggtg
    15241 tattctctcg ccagccctgg cacgtcgcgc aggcgctttt gatactctaa acagatacgc
    15301 tcgatcaaca ggttccataa atggttccct aaaaaatacc cggcggaatc agtatgcagg
    15361 cgggggtagc ccatgcgttc cagcaacgcg tcttcttcga accaggtcca caacatgggg
    15421 aagtcatcga tttcttccag tttaccaata tgaaagcgct gcaggcgctg gcggagagtg
    15481 gttttcttta aataatcaat gatctcgctc ttgcccgctg ccgggcgggc gatgagcagc
    15541 aaaatttcaa atcggtcagc ggcgggcatg ttttcctcct gtagatgcat cttgatgatt
    15601 gtgggcgaga tgtgacatac tgtcaagacc ccggttttgg caaatgatat aatcgagaac
    15661 accctccgcg ggggattttt tcaaccatga tcgaatctga aaccgctcac cgtaataaat
    15721 ggctcatcct gctggcgatc ggtatgggcg tgtttttagc caccatcgac ggtgggattg
    15781 tgaacattgg attgaacacg ctggtgaaag atttcggcac gccgttggca gttaccgagt
    15841 gggtcgtgtt ggcttatatg cttacaatct ccagcctcat gctttcaatt gggcggctag
    15901 gagacatgat tggcaagaaa tacctttata ttgccgggct ggccattttt actgttgggt
    15961 ctgtgctttg cgggatgtcg gagaccattt actggctgat cgcttttcgg gtactgcagg
    16021 cggtgggggc ttcgattacc atggcattgg gcacagcgat gattacagag gcttttcctg
    16081 accatcagcg cggcaaagcc ctgggcatta tgggaacgct ggtctctatt ggtatcattg
    16141 ccggcccaac gattggcgga ttgatactgg aacatttaac ctggcactgg cttttctttg
    16201 tgaacctacc ggtgggcatc ctgggggtct tgatggtgtg gcactatgta ccgcttgacc
    16261 gcccgggaaa gaagcagtct ttcgattttt taggggctgg gttgttattc atcacgctca
    16321 gctcgttgtt attggccttg tccaggatcc agagcgcagg tttgaccgac cctttgatcc
    16381 ctggcctgtt attccttttt ttgatcgggc tgattgtttt tatccgcgtg gaaaagcgca
    16441 gcgctgaacc gatgatcgat ctctcgatgt ttcgcaaccg tatgttttct gtgaacctta
    16501 tcaccgggtt cttgaccttt gtcgttgggg caggaaccat gattctcttt cctttatatc
    16561 ttcaaaatgt attggggttt ggcccacagc aatcaggctt aatgatggct gtcacccctc
    16621 ttacagtggc tatcgtcgct cccttctctg gggcgttatc agaccgggtg agttcacggg
    16681 tgttaacttc cattgggttg ggggtaatgc ttgtcgggca tagcctgatt tcaacgttga
    16741 gcacggatac cagtgtaacg gggtacctgc tgcggtttat cccgctgggc attggcatcg
    16801 gcctgtttca ggcgccgaat aacaacgcga tcatgggatc ggccgcgaga gaacgctctg
    16861 gcgtggcttc aagcctgctg agccttacgc gcaccgtggg ccagaccacg ggaattgcgc
    16921 ttttgggggc attttgggag aggcgcgcca ccacgctggc aggagacgac gcgttgcaca
    16981 gcgcgcttgc ggcgccactt aaggcgcagg tgggagggct gcagcagacc atgtacctgg
    17041 tcattgccgt catcatcgcg gcgctggggc tctctctatg ggcgcttgct gcctggttga
    17101 gagagcgcag cccccaaaac gcctgaccag cgaacccagg gagaaaaggc agccatgaga
    17161 gcaagtataa ttaattgggt tgattccatt taaagacgaa aggacgctta tgatgcgtaa
    17221 atatttgatt tggattacga cgatcttcat gcttgcctca tttatgctgg tatcctgcag
    17281 tctgcctgag gttacccccc cggcggcagg tgaaacagcg ccagtggtct cgacccccag
    17341 tgagcagaca gtgccactgc ctgcccaggt gataacagcc acccctgctc aaacccggtt
    17401 gatcaaagcc agcgaggttg gcaggttgga gatttcacag cgcgtggcgg tgagcaatat
    17461 ccaggatatt tcctggtcgc ctgacagcaa caccctggcg ctggtcactc aaaatacgga
    17521 tgctgcccag aatcaggttt ttagcgttac cctgttaaac gcgaaggatc tctccaccca
    17581 ggccgtttac tctgcaaccg gcaaccgggt gacctccgtt gccgcggatg gaaaaaccgc
    17641 agccgtgatc aaccaggacc agatgtcttt ctcactggtg gataccagtg tgaaggacgg
    17701 cgcttcgcgc agccgcgtta ccgattttct gattggcaac gtgacgatat cacccgatat
    17761 gcgttacgtg gcggtgacca aagctgaatt ttgggaagtg atcctttatg attttaacac
    17821 gctggaagag gtgcgctttt tatccgggtt ctcaacggct gcccctgttt ttgacgcggg
    17881 ttttaaccac agcccacaat ggctggtgtg gcacgcccgc gccaccctgc agctgcagga
    17941 ggtggagacc gggatgttga gcaacctcta ctcgcacgag gatttcgtta ctgcttacac
    18001 gttatctccc gacgggatga tccttgccag tgcttccttc agatctacca acgacgcttc
    18061 agtacctgtg gtgatccttt gggatacctt gaatggaacg gaattgaagg tgctggaaat
    18121 cagccaaccg gtgaacgctc tgcagttctc gccagatggg aaattgctgg ctgtggcggc
    18181 agggagtgac ctgcagattt gggacgccgc tggcggaaac ttgctcacca cgctcactgg
    18241 gcattcagat ttcatcctgc aactggcttt ttcaccagac caacacacca ttgcttctgc
    18301 cgggcaggat aagcagcttt atttgtggca gattctggaa tagacaaggt ctgcatgact
    18361 gacccggcca taaacgaccg ggttttttct tgaaataatc cttgtggtat actaccagcg
    18421 actgttccag gctcaggatc gcatatgccg ctgctgaccg ctgccaatct ctccaagtct
    18481 tacggtgctc tggatatttt tccagagatt tctttcagta ttcctcaccg ggcgcgcatc
    18541 gggctggtag gcgccaacgg ggtcggcaag acgaccctgt tgcgcgttct gatgggcgaa
    18601 gaggaacctt ccagcggcac cattcacann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnac cctgttgcgc gttctgatgg gcgaagagga accttccagc ggcaccattc
    18781 acaaggcgcg cggtattcgc atcggttacc tgccgcagga agcagctctg gattcgtccc
    18841 ggacgttatg gggggaatgc atgactgttt ttgaaccgct gctcgcccta cagcaggagc
    18901 tgcacgccat ggaagaggct ctttcagcca acccggattc gactgacctg atgcaggtct
    18961 acggcgaacg gcaaagggaa tttgagcacc tgggcgggta tgaatttgaa gcccgcaccc
    19021 gcatcacgct ttcgggattg ggatttaagc gcgcggatga aaaacgcccg gtcaggcagc
    19081 tttcaggcgg gcagcgcaca cgggtgctgc tggctaaatt gctgctttca gaacccgagc
    19141 tgctgctgct ggacgagccc accaatcact tggatatttc tgcggttgaa tggctggagg
    19201 ggttttttaa agaatggcag ggagcggtgc tcattgtttc gcacgaccgt tacttcttag
    19261 accagaccac caccacgatt tgggaaatga cccccgcgct tgaagagtat cgcggtaatt
    19321 actccgcata cctggcgcaa cgcgaagagc gctaccagcg gcgactgcaa gaataccagg
    19381 ctcagcaaga atttattgaa aaagaagagg aatatatccg ccgcaatatc gctgggcaga
    19441 acacccgcca ggcgcaagga agacgcaccc gcttggaacg cctgttaagc gaagccaggt
    19501 taaccccgcc tttgcagcgc cgcacactgc gcatgcagct ggtttcttcc ggcagaagcg
    19561 gtgacctggt gctgcgcacg cgttctctca gcgtgggtta tgcggatgaa ggcaagccct
    19621 tgtttgaagt ggatgacctg accctcgttc gtggagaatg cgctgccatc attggcccca
    19681 acggcgcagg aaagaccacc ttccttaaaa cgattcttga acaaatcccc ccctacgcgg
    19741 gggaaaccgt attaggcgcc agcttgaagg tgggttactt tgcacaggcg cacgaagggc
    19801 tgcaccctga ttggacatta atggatgaga tacaggcgat gatgccgaaa tggctgccgg
    19861 gcgaaattag agactacctg gcgaagttcc tgtttaccgg agaagatgtg ttcaagacgg
    19921 ttcacctgct gtcaggtgga gaacgagggc gcctggcgct ggcctgcctg gcgttacagg
    19981 gcgccaacct gttgctgctg gacgaaccca ccaaccacct tgacctgcct tcacaggaga
    20041 tcctgcagaa catcctggga caatttcagg gcaccattct actggtttcg cacgaccgct
    20101 accttattga cgcgctcgcc acccaggtgt gggaggtgct gccggaacag cgcaaactgg
    20161 cggtatttga aggcagttat agcgaatacc gcgctttaaa gcaggcaaac ctgatgatac
    20221 ctgatgggag accgcagcag aaagtggggt taacccgggc gaaaaaaccc gctgtgggga
    20281 aaagtaaaga ccagttgcaa cgcatgaagg cgcgcctgcg cgcgctggaa gaagagatcg
    20341 cccgcctgga gatcgatgcg cgcggcgttg aacaacgttt aagcgataga ggggttgatc
    20401 ctggcgaggt gcagcagctc gccagcgaat acgcgcgcct gcaggcgcag cttgaagcca
    20461 aaattgaatt atggagcgat ttgggagatg agttgagcga gccataaacc ctggcattcc
    20521 tccgggtttt atcagcgcta tcgaaacaac ccgttcaggt gccccaatcc ctcaggtaca
    20581 ggaagtcata accgacagtg cgaccgctca gtttggcgat gggcggcatg acgcgtttga
    20641 actggttctt gcgcacgcgc tcgatgacag cgtgaacaaa tttcacctca aatccactgt
    20701 caacacattc ctgcggcgtg taacgctgat ctaccagcaa gtagagcagc tgatcaaccg
    20761 cagcgtaagt gaagcccagc tcatcttcat ctgtctgccc ggcccacagg tcggctgaag
    20821 ggggtttttg cagcagtggt tcagggacac ccaccgccgc ggcgagctgt cgtaactggg
    20881 ttttatacag gtcacccagg gggttgagcg cacaggcggc atcgccgtgc atggtggtat
    20941 accccagcag aatctctgtt ttattgctgg tgcccagcac cagacctttg aagacctcgg
    21001 attggtcaaa aaggatgatc atgcgctggc gcgccatcac attccccctg cggcggtttc
    21061 ccatatccgg atagcggttg aacaatggct cgaccatatc ggtgattgga atcgtgagtg
    21121 ttcgcactcc gagcgcgtca atcaccagct gagcgtgagc cagtgaatct gctgaggagg
    21181 tggcataagg catacgcacc gccagcacat tctctggttg taacgcttct gccgccagat
    21241 aacaggcgag ggctgaatcg agaccgcccg aaagccccag cacggcgcgg ctgaaaccgg
    21301 cacgcgtgat ctcagtatga atgaagtcag ttagaatctt gcgcgccagg ttggtataaa
    21361 tcgtcaggtc gggcatttgc tcctcctcgt gcttcttcag ccatctgcat tacgggtgat
    21421 aatccgcgtg atttcgcgct gcaccaggct ggtgcgttca tcacgcagca gcggcaggcg
    21481 ggtacgcgta cggcgcagct ggcttaggtc cagctcggcc agcaccaggg cttcggcgtg
    21541 ttccacacca ttcaccaggg gttctccatc tggtgaaaaa gcggaagaac cgccccaaaa
    21601 atggatacca tcttcgaaac cagcgcggtt cgtgtgcacc acgaaagtgg tgaacatgcc
    21661 ggcatacgcg cggatgatat cattcaccca acgagcggat tctgttctac caggggcagt
    21721 attgtgaggt gtgggggtga taccgcgccc aggagaggcg gaagtaaaaa gaaaaaggtc
    21781 agcgccatcc agccaaagca tatatggaga agaggcgtgc cagaagtcct cgcagatgag
    21841 catgccgacc ctgccgaaac gggtatcaaa ggcgcgcaca ctttcgccgc gggcgaagaa
    21901 acgaccttca tcgaaaaacc cataggttgg caggtaaact ttgtgatgga tatggcgcac
    21961 cgctcctccg gagaggaagg cggttgcgat atagaaaagg ttgcgttcgt cttcttctac
    22021 aaaacctacc tgaatatcaa tatcctggct ggcaacgagc aatgggttga aaaccgggtc
    22081 atctggcaat ggacgacagg cgacagttgg tgtaaggtct tgcaaagcgt aaccggtgag
    22141 agaaagctca gggaaggtga tgaggtcagc ccccgcgcgc ctggcttgtt cgattaagtc
    22201 caggtgtttt tccaggttct gttctacatt ccccagcacg gtgtttattt gcgccatcgc
    22261 cacgttcagt ttcatacatt ttcctctgac ttcattttat cctttttttt tgatcagatg
    22321 tcagcaacaa cagcacagac agaagcgcga aagccaccgc gaagattaaa gacagaaccc
    22381 catcgctcct gatacctcga atcgggaaaa ccacatctgc cctgaaaaga gaaaaaacaa
    22441 gcgtgtgggt aaaaaaaacc aggctgacca gcgaaaaacg aaaaccgggt cgtttgaatt
    22501 gggtgatatg ttcaatcaaa cccagcaaga ggagcaagga aagcgcggct accagctgca
    22561 gaggaaagcg gttgctcagc ataccagttt catcaagggc ggggtacgaa agaaatgcgc
    22621 cggaggggag cgtctgaccg taggccacgc cttcatacca cagacccagc cagatgagcg
    22681 ctgccgtagg cagcagcatg cggctggtgc tatcaagcgc tgacagtaca ttcaggcgca
    22741 gcgcagcact gcccaacaga gcgaaaaaca cagcgccagc gaacgcgcca aacgcgttga
    22801 acccgcccag ccagaattgc cccgcctgtt cggggtgcag cgagtagtaa aagggatgag
    22861 cgagcacata accaacccgg gcgcctgtga gcactcccag cagcgcgaac acggcgcaaa
    22921 tccattgcct gctctccaca ccagcgggaa tggagcggta aacacgccac agaccgatgg
    22981 cggcgccaaa accagccagg atggaaaaca agcgtaatgt gagcatgtga gtttggggtt
    23041 gaggagctaa cgctgtggca aaaaaataaa gcccgtcatt cgatggcttc tacccctgtg
    23101 atttctggga agaactggcg aacggtcccc tcgacccagc ctttaatggt ggcgggcgaa
    23161 tgcggacagc caacacaagc gccgcccatg cgcactttta ccactttccc gtcgaaagaa
    23221 acgaactcca cctcgccacc gtggtaggta ttgatatatg cgctcaattg ctcgatcaaa
    23281 ccgcgcagta gctcaagctc agaataagaa cagcaattgt cagacatcat cactcctcag
    23341 ggtttttacc ttccggacag gctatcacgg cgcgcagcat gtgcatgatc tggtcgtgcg
    23401 ccttggccgg ctgctgcgcc acagacatgg cgattttttc caataatacc atgcctgatt
    23461 cctgatgttg ttcgcaaaaa cgctgaagtt tataagcggc gatacggtat acccgggagt
    23521 cagtggtggc aaacactgag gaggaatagg tcttccggcc gagaatggaa gaccaaccaa
    23581 acacaccccc ggaagtcaat ttgccaatgg tcagctccgg accatcgtaa ggcacgtggg
    23641 tgatttccac ttccccggtt accaggatga aaagatgaac tgcttgctga ctctgacgca
    23701 gaatggtttc gccagcaggg taggtaaaaa cctcgatgat ggatctcaat tcatcctgtt
    23761 catccagcga aaaaccctcg aaaatctgta caggcagcgg gctaccatta agcatattta
    23821 catcatatcg attgtatgat atgccagata gtggagaatt tcccttatat cttgataaac
    23881 ctcaccccgt aaaatgggca gtgatgatac attgaaccct gacctttttt gcaagaacaa
    23941 cttgaccgta gggcgctcga ttcgaaaagg gtgtcgtttt ttcccgctgt ggcagggttc
    24001 aagtacgttt gggtaaatct atggtgtgga tgaaacgaat tcccacgtag aggaaggcgg
    24061 gcctggtaca gcaataaaca aggagttcct tccctcgcgc tggtgtcggc tgcaagctga
    24121 atggtttacc ccggcaccac cttctggccg ggagttcctt catgcattgc ctccacgtcg
    24181 ttcgttttta gctgttgttt cgtgcgggag tatgagcggg gatgaccagg gtgtagccgt
    24241 gttcttttag cacctccagc ggcaggttat aggcgacgct gagcaacccg gcatttaaca
    24301 cctcttctgg tttgcccaag gctgccagct ttccacccac cagcaggccg acctggtcag
    24361 caaaacgaga aacaaggtta aggtcatgaa tggcaacgac caccgttttg ggctgcatcc
    24421 ctttcacaag ctcctctttc gtgggatgcg ccaactgatg gatgcgctgc atcaggctca
    24481 cctggtactg gaggtcgagg tgcgtggtgg gctcgtccag cagcaacagc ggcgactctt
    24541 gcgccagagc gcgtgccagg aaaagccgct gctgctctcc gccagagagc tctgaaacgg
    24601 cgcgcgagcg cagcgccagc aggtccatct tctccagcgc ctcttcaatg atcagcatat
    24661 ctttcccgtt gaccttgcca agccaattta aatgaggtgt ccttcccagc gccaccactt
    24721 cttcagcgct gaacgccggc gggatgctgc gcgcctgcgg taccaccgcg ataatgcgcg
    24781 cgcgctgctg cggagacagg ctgtagatat cctgaccagc cacttttact gacccggcgc
    24841 gcaagggtaa aacgccgctg atggcccgta taaacgtggt cttgcctgag ccgttggggc
    24901 caatcagcgc cagcacactt ccctggggaa gttgtaatga gatatcctca aggacaagct
    24961 cgtgatagta acctgcgctg agcgcgttaa cttccagcat ttttttcacc aatagttctg
    25021 tgctttcgcg agttttagca cccagagaaa aaatggcgcg cccagcagag cggtgacaat
    25081 tccgacgggt atctcttgag gtgcgataag cgaacgcgcc accacgtccg tcagcagcaa
    25141 caggctggca ccgcccagca tggaaagggg gatcagccgc cggtaatcgg ttccccaaag
    25201 caggcggatg atatgcggca ctaccagtcc cacaaaacca atgatgccgg caaaagctac
    25261 agcggcagct gttgaaagcg tggccagggc gatggtgatc cagcgcgcgc gttgcacccg
    25321 gatacctaat tgcagcgcct gttcctctcc gaactgcagc acgttaagcg ggtgaccgat
    25381 gagcaccaac ccggcgagcg cgatgagcat gtacgnnnnn nnnnnnnnnn nnnnnnnnnn
    25441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25501 nnnnnnnnnn nnnnnacgtt aagcgggtga ccgatgagca ccaacccggc gagcgcgatg
    25561 agcatgtacg gcagcacagc gaggatgggt gaccagcctg tcattattgc gccgcccatc
    25621 agccatacca gcgcgcggcg cagctcgcca gtggaatgga tcatcaacgc ggaggttact
    25681 gccgaggtga acgaactcac cgccacaccc gccaggatga ggttggtagt ggggacggtt
    25741 ttacccacac gcgctaacag ggtcacaatg agcacggtga gcagcgcgcc gcaaaaggct
    25801 gccagcggaa cggaaagcag cccgccccgg gtataaggcg aacgtgccag catggccagc
    25861 acggccccca acccggcgcc tgaagaaacg ccgattaaaa acggatcagc caacggattg
    25921 cggaacaacc cctggtaggc ggcgccgctg ccggcgagcg ccgcgcccac catcgttaac
    25981 atgagcatac gaggcaggcg caggtcgctg ataattttca ggtaaacgcc gttcacatcc
    26041 agggttgagg tcgcgccgcg cagggcggat gtcaccacat cccacagagt cggtagaggg
    26101 atgaacacgc tgcccaccgc cacactgagc agcgccgacg cagcaagcgc cagtgcgagg
    26161 accaggaaag gtgagcgcgt gttcttttta cgcatcaata aagctcgggg tgaatcaacc
    26221 tggccaattc ttccaggccg tccaccagcc ggggtccggg gcggctgacc aggtcatcat
    26281 tgaaggggta aacccggccc tcttgaaccg cgcgcaaacc atcccaaccc ggtcgggcag
    26341 ccacctgctc aggggtcatg ccgtagatgg aatcgccgag gaggatgata tcggggttct
    26401 gcaccagcag ttcttcgatg ctgatctgtg cccattgaga ttgcatcgcc gccgcggcgt
    26461 tacgaccgcc cgcttcgttg atcagaagat ccagaaagga tcctggtccc ggcgtccagg
    26521 gtttggcagg gtcagttccg tccagctcat aaaacaccac tggttttgtc tccgcctttt
    26581 ttatcgcctg ttgtaccagt tgaactcttt ctttaagtga ggaggtgagc gccgcggcct
    26641 ctttcgtgtg accgctgagc gcgcctaccg tttgcagcaa accgtaaata ccctcgaggt
    26701 cagtggggtt ggagagatag taaaccttca cccccaagcc ctcaagtgcg ctcacctgtt
    26761 caggggtatt gatctccgcg gcgatcacca ggtcaggatt aaggctggcg atggtctcaa
    26821 gtgaatagct tcccattgaa ccgccaacat cctggatgga tgtgacttct tcagggtagt
    26881 tggagaaagt gtcgcgcccc accacctggc tgcccgcgcc cacggcgaac agtatttcag
    26941 tgaccgaggg agaaagagaa atgatacggg cggcgggttt ttccaggaga acctcgcgcc
    27001 ccagtccatc ttgcagttgg atcttgccgc cgtcctttgc cgcgggtatg gcggaacagg
    27061 cagccagcat cagcaacaag agaaaaacag cgattcgtgt aattcgtgcg tgtttcattt
    27121 ggtcattcac ctctactatg attagattta gtaaaaaaac ccaaccgcta gggttgggtt
    27181 tcgcgtgtat tgctgcgagt gcccacctcc tttcagcgag agcgtggtaa cacccttttt
    27241 ggcggtcgac ctggcttatc aggaggtcct gattcacagt tgcgcgacag caccggaata
    27301 tgcaccggtt tcgcctttga gccgccctgt tcgggttagg caccaaaaag gtcagattga
    27361 ttactaagga ttctacccgt gattcaaaga agggtcaagt gatttttaac aggtgatggg
    27421 gcatttcaaa caaccggtca atcacagggg gcggctgtat aattaatgta tatatggagg
    27481 agccgtgaca cgtgtacctt acgaaattcg agtgggtgaa ttgttaaaag agaaacagaa
    27541 gaagctggtg ctggcggaat cttgcaccgg cgggctggtg ggaaacctga ttaccaacat
    27601 cgcgggctcc tcggattatt acctgggcag cgtgaccgcc tacgcttacg aggcgaaaga
    27661 agcgctgctg ggcgtacggc acgaaacgct gctgcaatat ggcgcggtca gccgccagac
    27721 ggtgctggaa atggctgccg gcgcacggcg ctcgttggcg ggcggcattc cgctggattc
    27781 aattatcggg gtctcgatca gcggtatcgc cggacccggc ggcggactgc ccgataaacc
    27841 ggtgggtacc gtgtggtttg gcatgagcac agcagatggt gattgggcct ggcgctgcat
    27901 gttcaaaggc aatcgtgtgc agaacaagcg ctctgccgcg cgcaccgcgc ttaaattggt
    27961 gattgcctac ctggcgggca gcctgccgcc tgaaaccaga tcacagcgtc attaagggtg
    28021 tccgatggct aaacgactga tcaggatact catcttaacc gcggatgccg gttttggtca
    28081 ccgcagcgct gccaacgcag tagccaaagc cttgaagctc aagtatcaat ccgcggttga
    28141 agtgagggta gaaaacccgc tggacctcga gagcgcgcca tttttcttgc gtgaagcgca
    28201 aacagattat gaccgctggg taaaacaggt tcccgagctt tataaactcg gttaccaggc
    28261 gagtgacgca gcgattccaa cacgactgct tgaagattct ctggccgttc tactgtttga
    28321 agcgattcgc gacacatttt ctgggccact gcctgatgtt gtgctcacca cctaccctat
    28381 gtaccaatcc gcgatcatgc tgctttaccg gttaagaaag atacatatcc cgctttttac
    28441 cgttgtgact gacctttcca ccgttcaccg attgtggttt aaccgcaacg tagacggctg
    28501 cctggtgcct aatgagattg tcgccaacct ggcggcagtg aatggggtcc ctcaggataa
    28561 agtgcacatc actggcatac cggtttcacc agatatcctt tatgaaacca gaccggcaga
    28621 gaaaatccgc gctgaacttg ggtggcaccc cgagctgacc accattctgg cggtgggcag
    28681 tacgcgcaca gaaaaattgc tggaggcggc gagcgtgatc aaccactacg gctcagaatt
    28741 acagctggtg gtggtcgcag ggaaaaatga agagctctac caggagctca accagatcgg
    28801 ctggcacatc ccggtacagc tgtttgattt tgtggacaaa gtaccgctct tgatgaaggc
    28861 atctgatttg gtcatttgca aggcgggagg actgattgtc acagaagcgc tggcctgtgg
    28921 attaccgatg gtactcacag atattatccc gggccaggaa acgggcaacg ctgaacttat
    28981 ccagaggctg caggcgggcg tggtggccga aacccccctg atgatgatgg aagccctgca
    29041 tcattttctg atgaatgatc aaaaaatgct tcggcaatac gctgacaatg ccagaaccat
    29101 tggcaaacca gcggccgcct gcgatgttgc ggagatcctt tttgaagctg gaaaggcgag
    29161 cacacctccg aaagcgtctt acagagcgca cggtttccgc tctgcggagc aacccctgga
    29221 aggcgatagc gaatgaagga gaatggtgag gttgaggtcg ccatcctatt ttcggcccat
    29281 tctgagtggc aaaccgcgca gtctctcttc ttggataccg ggaatgaaaa ctcaccattt
    29341 ggcgattatt ttttaacctg cgttgcagaa aagcgctgtg tctttttcca cggaggctgg
    29401 ggcaagattt cagccgcggc ttcaactcaa tttgtgattg atcgctggca gccgaaatta
    29461 ctggtcaacc tgggtacctg cggcgggttt gaaggacata ccgctgtcgg cgaggtggtg
    29521 ctggtagacg agacaatggt ctatgatatc cacgagcgga tgaatgaccc gtttgaagcg
    29581 cttcagttct ataccacgcg catcgacctg tccttcttgc gcaggcctta cccgcaggct
    29641 gtgcgcatcg gtcgtttggt atcagcggat gaggatatcg acccggccaa gatcaaccgc
    29701 ctgcgccagg agtttggcgc ggtagtggct gattgggaat ccgctgccat cgcatggacg
    29761 gctcagcgca acggggtgag ggtgctcatc ctgcgcgctg tgagcgacct ggtgagcgag
    29821 agcggcggcg aactctat
//
LOCUS       DAOSPM010000010        28275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1675_length_28275_cov_13.384089, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000010 DAOSPM010000000
VERSION     DAOSPM010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 28275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28275
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1675_length_28275_cov_13.384089"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLW25_01990"
     CDS             <1..465
                     /locus_tag="PLW25_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOS53292.1"
                     /translation="EADSVVALSGDTGDRVAEAARLYQKQYASYLFITYTDEPARDAL
                     IRAAVIEGIPADRVIVTEMQVSNTVDEARAIKALAKERAQDSLIIITDPFHTLRTRII
                     FRNVFRGSGIDVQVRPVGGHWYRSTTWWRTAEGRRYTLEEYLKILLYFFGRY"
     gene            505..1146
                     /gene="udk"
                     /locus_tag="PLW25_01995"
     CDS             505..1146
                     /gene="udk"
                     /locus_tag="PLW25_01995"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOS53293.1"
                     /translation="MTKHKKPLVIGIAGGSGSGKTTAADIILKKVGSHRIAYLQHDSY
                     YRELTNLPINQRREVNFDHPDSLESDLMREHIMALKEWKAIEVPVYDFTTHSRTSQTL
                     HVEPRRVILVEGILIFAEPALRSLFDVKIFVDTDADIRFIRRLKRDLEERGRTTEMVI
                     KQYLTTVRPMHLDFVEPSKRYADVIIPEGGLNEVAMDMVIARIESMLRDDSNP"
     gene            1143..>1662
                     /locus_tag="PLW25_02000"
     CDS             1143..>1662
                     /locus_tag="PLW25_02000"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOS53294.1"
                     /translation="MSKLSGWKLTFVRVLVLLAVITLTIFLILNRDRVQELEALGYPG
                     IFLISLFSNATLILPVPGVLFTSAMGAVFNPYWVALAAGCGAALGELTGYVAGFSGQG
                     IIENKQWYARVTEWMKKFGGLTVLLLAFIPNPIFDIAGWWRARCGCRCGSFSSLAGLE
                     RPAKCWFLRWVER"
     assembly_gap    1663..1762
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1763..4420
                     /locus_tag="PLW25_02005"
     CDS             <1763..4420
                     /locus_tag="PLW25_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOS53295.1"
                     /translation="AEVLRVQLGLIGTKVGCNEGHCGSCSVIMDGKLTRSCIVRMNKV
                     PDWACITTVEGIGTPKDMHPIQKALVFHGAAQCGFCMPGFVVSSKALLDVNPSPTRDD
                     VRAWFQKNLNACRCTGYKPIVDAVMDAAAVMRGEQPMASLEFKMPEDGRIWGKSYPRP
                     SAVAKVTGTADYGADLGLKLPTSTLHLALVQAKVSHANILEIDTTEAEKMPGVFKVVT
                     YKDIKGKNRINGLVTWETNRCDGWDRPILCDQKVFQYGDAIAIVCADTKAQALAAAEK
                     VRVELEELPAYMNAPEAMADDAIEIHPGTPNVYFTQGVVKGEDTKPLMASAAYTAEGS
                     FYTQRQPHLTIEPDMGFAYYDDEGRLTIHSKSIALYLHALMIADGLGVDASNIRMVQN
                     TAGGTFGYKFCPTIEALVGVACIATQRPVYLEYDYEQFITYTGKRSPFFTWLKLGADK
                     DGRLIAMETDWIVDHGAYSEFGDLLTHKGAQFIGAGYDIKNIRGRGRTVCTNHAWGAP
                     FRGYGAPESEFPSESLMDELSEKIGMDPLEFRYLNVYRPGATTPTGQVPEVFTLPELI
                     DLIRPKYQEAKKRAAAESTAEKKKGVGVAIGIYGAGGDGPDTAEAWIERTPYGVLVST
                     TWSDHGQGADAGALGTAYEALRPLGLRPEQIRLELNDTGKAPDGGAAGGSRSQVVTGN
                     AIKVCCENFLAAIRKPDGPYLSYDEMKEDDMPMRYSGVWTSPTEIGDPDTGQGNPIAN
                     YMYGVFLAEVVVDIKTGKVTVEKMTLAADIGKINNKAVVDGQLYGGIAQGIGFALSED
                     FEDIHKHTNMVKCGIPFAKDVPDEIELIYLETPRPSGPFGASGVGELPNTAPHAAILN
                     AVYAACGARIRELPAYPAKVLAALQQTSQ"
     gene            4503..6812
                     /locus_tag="PLW25_02010"
     CDS             4503..6812
                     /locus_tag="PLW25_02010"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS53296.1"
                     /translation="MDQRRLHELEEQCIQDCDPPCTSRCPVHVDVRQMCKEISAGDFK
                     HALITFQKSVPFPEIIARTCDQPCQALCNREPLGGAIRMADLELACCQLGMEESLLSP
                     VTGRKNALVSVIGGGLCGMTAAWELARKGYRVVLFDQAEQLGGQLWGLTDAQLPKEVI
                     LRESAKLKNKGIDLRLKTRVDLNAFDWAQYDAVLIATGVDQESVNWLEKNRQGNTVID
                     PVTFESGMSGVFCGGSLLHPPSTITSVADGKRTATSIDRYLQKVSLTASRSSEGTQDT
                     RLYTNLAGVLPVFAVPPADALQGYTMEEAIAEASRCLVCECMECVKVCTYLSELGAYP
                     RKYVRDIYNNLSIIMRQRRGNKFINSCTLCGLCGEVCPTDLNFAEVIVQARKTMVETK
                     KMPISAHDFALRDMNFSNGPRFALTYPAPGEGKCDYLLFPGCQLAASNPEYISLIYDD
                     LVRLVGNLGIMLRCCGAPAQWSGEETLFAESGAVIRSVWESLGKPKMILACSSCHQSL
                     KQLIPGLETVSLWEMLLKNEALGTTRGGCTQEYKVHDPCTSRYEPDWQHYARAVIERL
                     GVRYSELPMSRTLTECCGYGGVAWLANPDLVHKMIQRRINEDNANYITYCVMCRDLFV
                     SEGKPTLHLLDFIYGRDISALAIRKPPDYSQRHENRIRVKQRMEKRLLVKGDWKMETY
                     EKINLQLSSEMRDLLEARLILVEDLQKVIEHAEASGEFFFNKNNGHSIASFRPNLITY
                     WVEYEKQENSYTVFDAYSHRMVVGGDTSI"
     gene            7018..7782
                     /locus_tag="PLW25_02015"
     CDS             7018..7782
                     /locus_tag="PLW25_02015"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS53297.1"
                     /translation="MIADCATCGLFSFQPNPGICVHTHRPGGLVITERALALCDLPER
                     ARLLDVACGAGATVNFLSSKAGLKVTGLDRSGKALSGIQGKPILLNLVRSDLLNIPMA
                     PEYFDGVFMECALSLTGEPLNSLLEIRRVLKPGGKIVLTDIYLREVMTETIQRVLAGS
                     ACLAGAMTQDGVEEVIAAAGLTRLVWEDHSPLLKQWLVEHIFRLGSVSGLFQQLTREN
                     EQNERAAQALSRVRLGYYLAILEKPLSFKSQEAYDG"
     gene            7772..8212
                     /locus_tag="PLW25_02020"
     CDS             7772..8212
                     /locus_tag="PLW25_02020"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOS53298.1"
                     /translation="MMDDLSKIRRFGEQGFYCSQVILLMGLEWQGKEDPALVRSMHSL
                     AGGIGFSGHNCGALTGGACLLGYFAGRGSTSEEEDDRLFLMLNELVDWFTRKYGVEYG
                     GIDCENILSGGKDQYVARCPGIVQSTLQKCKELLVEYGYELNGE"
     gene            8187..9575
                     /locus_tag="PLW25_02025"
     CDS             8187..9575
                     /locus_tag="PLW25_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS53299.1"
                     /translation="MAMSSMVSEIPVEGCVICRTQSICPVCLNRLEAKRVEYEDGVYL
                     EKHCREHGFFRTILWRGRPDYGSWRREKLPNHPRHPFTQVERGCPYDCGLCAEHRQEP
                     CCVLLEVTSRCDLDCPVCFASSGEKDATDVPLSTISLWYERMLKAGGPFNIQLSGGEP
                     CVRDDLAQIIAMGKKAGFSYFQLNTNGLRIARERDYLASLKEAGLGVVYLQFDGTTDD
                     IYRTIRGREILDAKKKVILNCRALELGVVLVPTVVPGVNDKDLGNIVRFALENYPVVR
                     GIHFQPISYFGRFPDQPRNEDRITIPEILRNLQEQTEGLVKTEDFHPKGSENSYCSFH
                     CTYILMPGGELLAVKNQAGKTCGCREPEDSSKALLRTRSFVARNWIYPTPSAPCSCGE
                     SASMGEWDVILERSKTHLFSVSGMAFQDAWNLDLDLLQDCCINVAADDGKLIPFCAYN
                     LTNTLGEGLYRL"
     gene            complement(9900..10193)
                     /locus_tag="PLW25_02030"
     CDS             complement(9900..10193)
                     /locus_tag="PLW25_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53300.1"
                     /translation="MVKTGHPVQMNWGTNQYVHFFLPNDLQYTPFIINRAIFGGQAAQ
                     FTQGSEKQVSYAARPLARQQDQHSVPCTCQRGHARSKKVNGQRLVFLREKNPF"
     gene            complement(10907..11311)
                     /gene="rnpB"
                     /locus_tag="PLW25_02035"
     ncRNA           complement(10907..11311)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLW25_02035"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            11444..12202
                     /gene="tpiA"
                     /locus_tag="PLW25_02040"
     CDS             11444..12202
                     /gene="tpiA"
                     /locus_tag="PLW25_02040"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOS53301.1"
                     /translation="MRKPLVAGNWKMNKTVEQASLLARELLTGLQQVPEVERVLCPPF
                     TSLMILSAQLSGTGVGIGAQNMHWAESGAYTGEISPQMVREFCEYVIIGHSERRTYFG
                     ETDESVNRKLKSALALGLVPIVCIGETLEEYEAGVTGEVITRQVLEGLKDIGTASASI
                     IVVAYEPVWAIGTGRAATAEGANAVLRDHIRPALAQLFGSGLAQNLRILYGGSVTVAN
                     ATEFFRQPEIDGALVGGASLKVDEFVKIVEAARP"
     gene            complement(12254..12880)
                     /gene="upp"
                     /locus_tag="PLW25_02045"
     CDS             complement(12254..12880)
                     /gene="upp"
                     /locus_tag="PLW25_02045"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOS53302.1"
                     /translation="MSNLHVSAHPLVAHKLAKLRDARTEPKKFRELVREIAGLLAYEA
                     TADLVMEDIEVTTPLATASGKILKDKIGLIPILRAGLGMVEGFWDLMPGAEVWHIGIY
                     RDENTLKPVEYYNKLPTEPTVSVCLILDPMLATGGSATATVEVLKRWGVKRIKFVGII
                     GAPEGIAAMTKAHPEVPIHLAAVDERLNNVGYILPGLGDAGDREFGTA"
     gene            12979..14016
                     /gene="ruvB"
                     /locus_tag="PLW25_02050"
     CDS             12979..14016
                     /gene="ruvB"
                     /locus_tag="PLW25_02050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOS53303.1"
                     /translation="MGFMTADERIVNPVPKVDDRVDNVLRPSKLKELIGQDQVRQNLE
                     ILIAAAKGRKECLDHVLFYGPPGLGKTTLAYILANEMGVNIKVTSGPAIERTGDLAAI
                     LTNLREGDILFVDEIHRLGRSIEEVLYPAMEDFSLDIIIGKGPSARSLRLKLPRFTTI
                     GATTRLALVTAPLRARFGAVYRLDYYDLTAMRKIVERAASLLNVTSDDTGIEEIARRA
                     RGTPRVALRLLRRVRDFTQVRANGLITRETAINALDLLAVDAMGLDDVDRRVLKTIIE
                     KYNGGPVGLATIAASISEEADTIMDVVEPYLLQLGYIDRTPQGRVATPLAYEHLQLPY
                     NQKDNQPRLIP"
     gene            14019..15038
                     /gene="queA"
                     /locus_tag="PLW25_02055"
     CDS             14019..15038
                     /gene="queA"
                     /locus_tag="PLW25_02055"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOS53304.1"
                     /translation="MQTADFDYHLPPEYIAQTPIEPRHASRLLVLRRSSATLEDSHFC
                     QLPDFLNPGDLLVINQTKVIPARLHARKTSGGKVELLLLKQREPGLWEALAGGKGLRK
                     GVRVQLENGPYVDIEEELTGSERLIRFSEPVEQLLPTIGEMPLPPYIHEKLLRPDRYQ
                     TVYATETGSAAAPTAGLHFTPWLMDEIERKGVRIAKVTLHVGLDTFAPVTELDPVEHK
                     IHTEWCELTEEAALLINRTKASGGRVVAVGTTSVRTLESAAATSNQPSWVAPYRGQTG
                     LFILPGFEFKVVDALVTNFHLPKSTLLMLVSAFAGRERVLDAYQAAMERGYRFYSFGD
                     AMLIL"
     gene            15047..15772
                     /locus_tag="PLW25_02060"
     CDS             15047..15772
                     /locus_tag="PLW25_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOS53305.1"
                     /translation="MTCPRSLKLLDNQIIACRTCPRLVAWREKIGQEKRRAYLGQTYW
                     AKPLPGFGDPLARVLVVGLAPGAHGGNRTGRMFTGDDSGDFLFSALHRAGFANQPEAK
                     ALNDGLMLNDVYITAVCRCVPPDNKPLPAEISNCLPYLQCEFELLEKVEGVVVLGRIA
                     FDTVSKLLYHQRKLATSAIFGHGVLQPSTDGAPWLLCSYHPSRHNTQTGRLTTTMFDQ
                     IWEQVNRLLGKDELLSCQEPGSS"
     gene            complement(15740..16498)
                     /locus_tag="PLW25_02065"
     CDS             complement(15740..16498)
                     /locus_tag="PLW25_02065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS53306.1"
                     /translation="MKLYDSFASVYASGDYPNLSQRMAAILPGVMKHYKILTNNRKKL
                     LDVACGEGSFCVEMAKNGWSVTGVDQSEEMLRLAKHRAKNDQVDVKFEKQDMRFIDYH
                     NEFDLATCWFDSLNYLISNNDLQSTFNSIARSLKPNGWFLFDMNTIYGLAVEWQKNRC
                     YVRQETKDLLELHRTCYDFEKNLGCIKITWFVKVDDKWEKFEEKHIERGFSIAEIESC
                     LEYAGLHVVDKVSSLDPISPLGSQSGRAWFLTRK"
     gene            complement(16651..17937)
                     /gene="eno"
                     /locus_tag="PLW25_02070"
     CDS             complement(16651..17937)
                     /gene="eno"
                     /locus_tag="PLW25_02070"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOS53307.1"
                     /translation="MEIASVEALEILDSRGNPTVEVRVILEDGSIGTAAVPSGASTGI
                     HEALEMRDGDKNRYNGKGVLQAVEHVNGEIAENLIWWDAIDQKAIDQMLIELDGTPNK
                     SRLGANAILGVSLAVAKAAAASLDLPLYRYIGGVYAHVLPVPMMNILNGGAHTGWQST
                     DAQEFMVMPLGAPSFAEGLRCGDEIYQALKGVLKSRGYPTLVGDEGGYAPALKANSEA
                     VEVILEAIEKAGYKAGEEVAIALDPAASEFYEEETKTYNLRKEGKKLSGEQMVEFWLN
                     WVRQYPIVSIEDGLAQDDWDSWKLLTKEIGHKVQIVGDDLLVTNTSRVRRAIKEEACN
                     ALLVKVNQIGSLTETIEAVEACHRAGWRAVTSHRSGETEDSTIADLAVALNTGQIKTG
                     APARSDRVAKYNQLLRIEAELGETAAYAGWSVLKAY"
     gene            complement(18025..19419)
                     /locus_tag="PLW25_02075"
     CDS             complement(18025..19419)
                     /locus_tag="PLW25_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOS53308.1"
                     /translation="MPKPRLLIPVFTHFSVRYILRTGMLTALTEFAQPIVALSWQDPA
                     LENEFSQAGAEILHVPNFTFSPQFKETRELVNFWHKQVRQTNTTEIDIRREASLLGLR
                     ERVNYEVQILKDRIKTALPARIPHLLQQEESLFWSETNYQEFDAILDQSKPDAVFSIT
                     PFVNKEEPLLRCAQKRGVLRITSILSFDNVTSRTRLPVSFEHYLVWNRYNHAELLRAY
                     PDITPEQITISGPPQFDFYYDSANVIPEEKWRSRLGLPSARPVILFGGGTYKIVPNEP
                     LFLKQLDDAISNGEVPAHPIILFRRHPGDIASRWQEVLSGVKNTFTDQSWQANESRGQ
                     INITHADIEQLCSTLAHCAVQINSSSTLTVDGAVFDRPQIGPAYDPGSSRKYDRVLKE
                     LYIREHYIPITNSGGLDLVYSRPDFIRAVNDGFEKPAERTEGRKKIVEEICTYNDGQS
                     TLRVVKKLRQLLGG"
     gene            complement(19498..21141)
                     /locus_tag="PLW25_02080"
     CDS             complement(19498..21141)
                     /locus_tag="PLW25_02080"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOS53309.1"
                     /translation="MTTKYIFVTGGVVSSVGKGVTAAAIGLILKERGYKVAVQKLDPY
                     INVDPGTMSPYQHGEVYVLDDGAETDLDLGHYERFIDIRLNRVCNITTGQVYAEVIAK
                     ERRGDYLGGTIQVIPHITNEIKRRINLVSKSTSAEIVIVEVGGTVGDIESLPFLEALR
                     QLRSDLGRENTVNVHVTWLPYIGATDEIKTKPTQHSVAELRSIGIAPDMIVARSDHPV
                     AENLREKIAQFCDVEKRAVIPMVTSPILYEIPLLLEKEKVGEYLLHRLNLDSKQQPDM
                     SDWEKLVSETAREKPRVSVALVGKYVELHDAYMSVREALKHAALSLGLELDLRWVHSA
                     DLEKAKHVNALEDAHGIIVPGGFGSRGIEGKIWAANYARVNLIPYFGLCLGMQLMCVE
                     FGRYALGSDQVNSAEFDLSTPHPVIDLMPDQQNIKDVGGTMRLGLYPCILQPGTIAAN
                     AYGAKKVEERHRHRFEFNNAYRERMAQRGMVFSGLSPDKNLVEIVELKDHPFMLGTQF
                     HPEFLSRPTRPHPLFLAFMRVAAVKAGVAYRDPPLLYKR"
     gene            complement(21331..22158)
                     /locus_tag="PLW25_02085"
     CDS             complement(21331..22158)
                     /locus_tag="PLW25_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOS53310.1"
                     /translation="MNAETQDNQQLEPKMPFKARVIKVSLAGAVLDIGIGKPALLHVS
                     QIIAPDNTQNKKVDELLKEGEEIDVWVKKLTRKDSEERIEMTMFKPLDLEWREIKVGE
                     TVKGKVVRLEKFGAFVEIGAERPGLVHISEMAHGYVKLPSDVVQEGDEVEAQIIEVNR
                     RKKQIKLSMKAIQPEPEKVVEEKPAPAASGAEGARREKKSARKPRRKSEVESSGVLEV
                     KNETVEPEPTYMELAMRAAMEKAKQKKKKQEVHRTKPGSTEQDEILARTLENKVQTN"
     gene            complement(22259..23527)
                     /locus_tag="PLW25_02090"
     CDS             complement(22259..23527)
                     /locus_tag="PLW25_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOS53311.1"
                     /translation="MNTNHTGRIILVIMISLVLLAGAFSGGVILGWLIPSGSPLETLA
                     PDTTKPAETPPLPDSTPPSSTPADLRKLFQPFWEAWDIIHEQYVDQPVDDLQLMRGAI
                     QGMLNSLGDIHTGYMTPEDYDQATTTLDGSYGGIGAWVDTAGEYLTIIRSMSGSPAEA
                     AGLKPGDEIIGVDGQSVIGKDPSAVLQTVKGPEGTTVVLTVQRHAPEEILDFTIVRAQ
                     ITVSSVESRMLDQGIGYLALNTFGSESAQEMETALAGLLAQHPVGLILDLRDNSGGYV
                     DAAIDIISQFIGSGVIMIERFGDGKEVTFEAHPGGLATEIPLVVLVNQYSASASEITA
                     GAIQDYQRGKIVGTTTYGKGSVQNWVPLADEQGAVKVTIARWLTPKGRLIHGIGLTPD
                     YIVERTENDIEAELDPQLEKAIDLLLNANK"
     gene            complement(23809..24462)
                     /locus_tag="PLW25_02095"
     CDS             complement(23809..24462)
                     /locus_tag="PLW25_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HOS53312.1"
                     /translation="MSFAVMLFDLDDTLYPSTSGVWDAIGDRMDSYIIKTLGVAANEV
                     KQIRNGLFHEYGTTLRGLKTLYHIDECEFLDYVHDIPLTQFLRKDEALIETISAYDSR
                     KVIFTNANQRHAERVLEVLGLVDFFPEIVDVLQISPYCKPLPQAYQKAMEIIQIEDPE
                     HCVFIDDSPRNLKTAHEMGFYTIQVGTENRCEFADAAILSLLDLPDVIPSSHRTMER"
     gene            24537..26288
                     /gene="mutL"
                     /locus_tag="PLW25_02100"
     CDS             24537..26288
                     /gene="mutL"
                     /locus_tag="PLW25_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOS53313.1"
                     /translation="MSIQVLSEELASQIAAGEVVERPASVVKELLENAIDAGASRISI
                     RVEDAGKKKIEVSDDGTGIPIDELPLAVSRHATSKINTAEDLFHIRTLGFRGEALASI
                     GSVSRLMVLSRNADSQVGARIMVEGGRIVSKEMTGSVTGTIVRVSDLFYNTPARLKFL
                     KRDVTERQQIDTLVSRYAMAYAGKAIHLEHDGKAVLRTSGNGERREVLAQLYGIELAR
                     QLLEVVFEEEGMKIEGYISPISISRSNRKDMTFFINGRWVQDVTLVSALLKAYQTYLM
                     VGRYPLSILFIEIPPEAVDVNVHPAKAEVRFREPDRVFTAVQRAARRALMAYVPIPPL
                     AAASWSSRGWNPSPELDGTWMNARPVQPQSTGQPQPMAETPLSSSVEPAPFAAGIPLL
                     RLVGQIGATYLVAEGPDGLYLIDQHAAHERVLYEKMQRQKENIPTQPLLEPLLIQLPP
                     QSAQIICDNLQVLRKIGFHLEEFGRNSFRLSAIPALFTQKDPRAAINSVVEDFEEDEE
                     PLKEVHEDRLIARICKRMAVKGGSILSTEEQQALLRDLESCSSPRTCPHGRPTMIHLS
                     VELLERQFGRRGARSAF"
     gene            complement(26285..27058)
                     /locus_tag="PLW25_02105"
     CDS             complement(26285..27058)
                     /locus_tag="PLW25_02105"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOS53314.1"
                     /translation="MTTPVEHELRRAADLIASSKYFVAFTGAGISTPSGIPDFRSAGT
                     GLWQRDDPMKVASATAFRQSPRNFYNWLRPLLRVSYSAHANDAHLALATLEKAGKLQA
                     VITQNIDELHQKAGSVNVVELHGSIHRFYCPANKEHGVDFAAVVTSILSGEIPLCATC
                     GTVLRPDITLYEEELAADDWLKAENEIALADLLLVAGSSLEVTPAALLPFKACRNGCK
                     IILVNHSATSIDPYAELVMRSDVAECIPAMVALLNQRCG"
     gene            complement(27055..28209)
                     /locus_tag="PLW25_02110"
     CDS             complement(27055..28209)
                     /locus_tag="PLW25_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS53315.1"
                     /translation="MPEKILIVDDDMETLRLVGMMLQRQGYEIAAANTGELALETAVK
                     VKPSLIILDVVMPGMDGYQIARELRKNPLCTHIPILMFTAKNQMGDKIAGYGAGADDY
                     LTKPVHPAELNAHVKALLDRMKKIKNTPEESGSGCIIGVLGCKGGLGVSTLVLNLAVS
                     LSGMTSKEVVAVELKPGQGSWGVELDIANSYGLEKLLALPVDEITAAAVEKLAVHTSF
                     NVRLLMASEKSQKISLPDLPEKTLAIIKSLNSSIPVLVLDIGNPYLAGFSKVHKLCDQ
                     LIVLTDAFPATVLRTRKLLGTLQFEVPKKNRYIDTVFYNHIRSEAGLTAEQITKLLPG
                     TTIKFMIPPVPEQVFQANQKYIPLYHIQPEGLFSQQVNELSRAICQRFQL"
BASE COUNT         6629 a         6946 c         7689 g         6911 t
ORIGIN      
        1 gaggcggata gcgtggtagc actgagcggc gataccggcg accgggtggc agaggcagcc
       61 cggttatatc aaaaacaata tgccagttat ctcttcataa cctatactga cgaaccagcg
      121 cgggatgccc tgatacgggc tgcggtgata gaaggaattc cggctgaccg cgttattgtg
      181 acggaaatgc aggtatccaa cacggtagat gaagcacgcg ccatcaaagc gctggcaaag
      241 gaacgcgcgc aggattcact gatcatcatc actgatccct ttcatacgct gcgaacgcgc
      301 atcatcttcc gcaacgtatt tcgcggaagc gggatagatg ttcaggtgcg gccggttggc
      361 gggcattggt accgttccac cacctggtgg cgcactgctg agggtcggcg ttacacattg
      421 gaagaatacc tgaaaatttt gctgtacttt tttggcaggt attaaattag gatgctttcc
      481 gatggattct aatataatta ttccatgacc aaacataaaa aaccacttgt tatcggaatc
      541 gccggtggat cgggctcagg gaaaaccact gcagcggaca tcatcctgaa gaaggttggc
      601 tctcaccgca ttgcgtatct gcagcacgat tcatactacc gggaactgac caacctaccc
      661 atcaaccagc gccgggaagt gaactttgat cacccggatt cacttgagtc cgacttaatg
      721 cgggagcata tcatggcgct caaagaatgg aaagccattg aggtgccagt atatgacttt
      781 accacccaca gccgaaccag ccagacactg cacgttgagc ccaggagggt gatccttgta
      841 gaaggaatct tgatctttgc cgagccggct ttacgctcgc tttttgatgt gaagatcttt
      901 gtagatactg acgcggatat tcgctttatc cgccgtttga agcgtgacct tgaagagcgc
      961 gggcgcacca ccgagatggt aattaaacag tacctcacta cggtgcgacc gatgcacctg
     1021 gattttgtgg agccctcaaa acgctatgcg gatgtgatca tccctgaggg gggactgaac
     1081 gaagtggcga tggatatggt cattgcccga attgaatcta tgctgcgtga cgacagcaac
     1141 ccatgagcaa attaagcggc tggaaactta cttttgtcag ggtgctggtg cttctggcgg
     1201 ttattacatt aaccatattt ctcatcctca atcgtgacag agtgcaggaa ctggaagcgt
     1261 tgggataccc ggggatattt ctgatttccc tgttttccaa tgccacgctc atccttccgg
     1321 tgcctggcgt gctgttcacc tcggcgatgg gcgcggtttt taacccgtac tgggtggcgc
     1381 tggcagccgg gtgcggagcg gcgctcggcg aactgacggg ttatgtggct gggtttagtg
     1441 gacaaggcat tatagagaac aagcaatggt acgccagggt tacggaatgg atgaaaaaat
     1501 tcgggggact gactgtgttg ttgctggctt ttatacccaa cccgattttt gatattgccg
     1561 gatggtggcg ggcgcgctgc ggatgccgct gtggaagttt ctcctcttta gctgggttgg
     1621 aaagaccggc aaaatgttgg tttttgcgtt gggtggagcg ggnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nngctgaggt tctgcgtgtt cagctggggt taatcggtac
     1801 caaggttggg tgtaatgaag ggcattgcgg ctcttgttcg gtgattatgg atggcaagct
     1861 gacacgttcc tgtatcgtca ggatgaacaa ggttcctgat tgggcctgca ttaccacggt
     1921 tgaaggaatc ggcactccta aagatatgca tccaatccag aaagccttgg tctttcatgg
     1981 ggcggcgcag tgtgggtttt gcatgcccgg ttttgtggtt tcgtccaagg cgctgcttga
     2041 tgttaaccct tcgcctaccc gtgatgatgt gcgcgcctgg ttccaaaaga acctgaacgc
     2101 gtgccgttgt acaggctaca agcccattgt ggacgcggtg atggacgccg cggctgtgat
     2161 gcgcggcgaa caaccgatgg caagcctgga gtttaaaatg ccggaagatg gaaggatctg
     2221 ggggaagtcc tatccgcggc cttcagctgt tgccaaggtg accggaacgg ccgattacgg
     2281 cgccgacctt ggcttaaaat taccgacatc caccttacac ctggcgctgg ttcaggcgaa
     2341 agtgagccac gcgaatattc tggagataga tactactgaa gcggagaaaa tgcccggtgt
     2401 gttcaaggta gtcacttata aagatatcaa ggggaaaaat cgtatcaacg ggttggtaac
     2461 ctgggaaacc aatcggtgtg acggctggga ccgccccatc ctgtgcgacc agaaagtatt
     2521 tcaatatgga gacgcgatcg ctatcgtctg cgcggatacc aaagcgcagg cgctcgccgc
     2581 ggcagaaaag gtcagggttg aactggaaga gctgcccgcg tatatgaacg cgcctgaagc
     2641 catggcggat gatgcgatcg agattcaccc cggaacaccg aatgtttatt ttacgcaagg
     2701 cgtggtgaaa ggagaagaca ccaaaccgct tatggcatcc gcggcttaca cagccgaagg
     2761 gtctttttac acgcagcggc aacctcatct caccatcgaa ccggatatgg gttttgccta
     2821 ttatgatgat gaaggcaggc ttaccatcca ctctaagagc attgctttgt acctgcacgc
     2881 tttaatgatc gcggatggtt tgggtgtaga tgcctcgaat attcggatgg ttcaaaacac
     2941 tgccggcggc acttttggtt acaagttttg ccccaccatc gaagcgttgg ttggcgtggc
     3001 ctgcatcgcg acacaacgac cggtttacct ggaatatgat tatgaacagt tcattaccta
     3061 tactggaaag aggtcgcctt tctttacctg gttgaaactg ggagcggata aagatggaag
     3121 gcttattgcc atggaaactg attggattgt ggaccatggc gcttattctg aatttggaga
     3181 cctgttaacc cacaagggcg ctcagtttat cggggcggga tacgacatta agaacatcag
     3241 gggaagaggg cgaactgtgt gcaccaacca tgcctggggt gccccgttca ggggttacgg
     3301 cgcgcctgaa agtgagttcc cttccgagtc tttgatggat gaactctcgg aaaaaatcgg
     3361 catggatcca ttggaatttc gatatctcaa cgtgtatcgt cctggtgcaa caactccaac
     3421 cgggcaggtg cctgaagtat tcacgttacc ggaactgatc gatctcatcc gccctaaata
     3481 ccaggaagcc aaaaaacggg cggcggccga atctactgcg gaaaagaaaa aaggcgtcgg
     3541 cgtcgccatc ggtatttatg gcgcaggagg agacggtccg gatacagcag aagcctggat
     3601 cgaaagaacg ccatacgggg tgttggtttc caccacatgg agcgaccatg gacaaggcgc
     3661 tgacgctggc gcgctgggga ctgcctacga agcgctgcgg ccattggggc tgcgacccga
     3721 gcagatcagg ctggaactga atgataccgg taaagccccg gatggtgggg cggctggagg
     3781 aagccgcagc caggtggtaa ccggcaatgc cattaaggtg tgctgcgaga acttcctcgc
     3841 tgccatccgt aaacctgatg gaccttacct ctcttatgac gagatgaaag aagacgacat
     3901 gcccatgcgc tattctggcg tttggacttc accgaccgag attggtgacc cggatacagg
     3961 gcagggcaac ccaattgcga actacatgta tggcgtgttc ctggctgagg ttgttgttga
     4021 catcaagaca ggcaaagtga cggtagagaa gatgactctg gctgctgaca ttgggaagat
     4081 caataacaaa gcggtggtgg atgggcagct gtatggcggc atcgcgcaag ggattgggtt
     4141 tgctcttagc gaagattttg aagatatcca taagcacacg aacatggtga aatgtggtat
     4201 tcctttcgcc aaggatgtcc cggatgagat cgagctgatt tatttggaga cgccgcgccc
     4261 ttccggtccg ttcggcgcat caggggttgg ggaattgccc aacacagcgc cgcatgcagc
     4321 catccttaat gcggtttacg cggcctgcgg ggcgcgcatc cgcgagctgc ccgcgtaccc
     4381 tgccaaggta ctggcagcct tgcagcaaac cagtcaataa ccgccgggaa aaacctgtag
     4441 gtatggcgtg gaggtcaatc atcgtaccca tacttgctca aagcatctgg ttgggttgaa
     4501 aaatggatca acgccgttta catgaacttg aagagcagtg catccaggat tgtgacccgc
     4561 cttgcacttc ccgctgcccg gttcacgttg atgtacggca aatgtgcaag gaaatttccg
     4621 ctggggattt caagcatgcg ctgatcactt tccagaaatc agtccccttt cctgaaatca
     4681 tcgcccgcac gtgcgaccaa ccctgccagg cgttatgcaa ccgtgaacct ttgggcgggg
     4741 cgatacggat ggcagacctg gaattggctt gctgccaatt gggaatggag gagagcctgc
     4801 tttcgcctgt gactgggcgg aaaaatgccc tggtctcggt gattggaggc gggttgtgcg
     4861 gcatgacagc ggcttgggag ttagcccgca aaggatacag ggttgtgctt tttgaccagg
     4921 cagagcaact gggggggcag ctttggggct tgacggatgc acaattacct aaggaagtca
     4981 tcctgcgcga atctgcgaaa ctaaaaaata aaggcattga cctgcgttta aaaacacggg
     5041 ttgatctaaa tgctttcgat tgggctcaat atgacgcggt gttgatcgct accggcgttg
     5101 atcaagaatc ggtcaattgg ctggagaaaa accgtcaggg caatactgtg attgacccgg
     5161 tgacatttga gtcgggtatg agcggagtgt tttgcggcgg cagcctcttg catccgcctt
     5221 ctacgataac ctccgttgcg gatggtaaac ggacagccac cagtattgac cgctatttac
     5281 agaaggtgtc gctgacggct tcccggtcat ccgaaggaac gcaagacacc cgcctgtaca
     5341 ctaacctggc aggtgtgctt ccggtctttg ctgtgcctcc tgcggatgcg ttacaaggct
     5401 acacaatgga ggaagcgatt gctgaagcca gtcgatgtct tgtatgcgaa tgcatggaat
     5461 gtgtgaaagt ttgcacttat ttatctgaat taggcgctta cccacggaaa tacgtcaggg
     5521 atatttacaa caacctttcc ataattatgc gccagaggcg ggggaataaa tttatcaact
     5581 cctgtacgtt gtgcgggctt tgcggcgagg tgtgccccac tgatttgaat ttcgctgaag
     5641 tcatcgtgca agcgcgaaaa accatggtgg aaaccaaaaa aatgcccatc tcagcgcatg
     5701 attttgcgtt acgtgatatg aacttcagca atggcccgcg gttcgccctg acctaccccg
     5761 cgcccgggga aggaaaatgc gattatttgc tgtttccagg ctgtcagctg gccgcgtcga
     5821 acccagagta catctccttg atctatgatg acctggtcag gctggtgggt aacctgggaa
     5881 tcatgctgcg ttgttgcggc gcgccagccc aatggtcagg ggaagagact ctgtttgccg
     5941 aaagcggcgc tgtcatccgc agcgtatggg aatccctggg taaaccaaaa atgatcctgg
     6001 cctgttccag ctgccatcaa tcgcttaaac aactgatacc ggggttggaa accgtctctt
     6061 tatgggaaat gttattaaaa aatgaagcgc tcggaactac gcggggcggg tgcacgcagg
     6121 aatacaaggt ccacgatcca tgcaccagcc gatacgagcc agattggcag cattatgcca
     6181 gggcggtcat cgagcgatta ggtgtccgtt attcagagct gcccatgtcc cgcactctaa
     6241 ctgaatgctg cggctatgga ggggttgctt ggttggccaa ccctgacctg gtgcataaaa
     6301 tgatccagcg taggatcaat gaagacaacg cgaactatat tacctattgt gtgatgtgcc
     6361 gggatctttt tgtatctgaa gggaagccta cgcttcatct attggatttc atctatgggc
     6421 gggatatttc ggcgctggca ataagaaaac caccggatta ttctcaaagg cacgaaaatc
     6481 gcatacgtgt aaaacagcgg atggagaagc gtttgctcgt gaaaggagac tggaagatgg
     6541 aaacttatga aaagatcaac ctgcaattga gcagcgagat gcgggatctt ctggaagcgc
     6601 gattgatctt ggtggaagat cttcagaagg taatcgaaca cgctgaagcc agcggggaat
     6661 tttttttcaa taaaaacaat ggtcattcca ttgcctcttt caggcccaac ctcatcactt
     6721 attgggtgga atatgagaaa caggagaatt cttataccgt gtttgacgcg tacagccacc
     6781 ggatggtggt gggaggagat acaagtatat gacagcgtct gagatgaaca aaaactcgac
     6841 tgaatggatc tgtggcaagt gcggagaacc attacgcttg aacaaggtta atgtgagtta
     6901 cctcgggtca ggatatcccg tggatttatt gacgtgcccc aaatgcaacc gcgcgttggt
     6961 ccctcctgaa atggctctgg gtaagatgct cgaagtcgag aggttgctgg aagacaaatg
     7021 attgcagatt gcgccacctg cggtttgttc tcctttcagc cgaaccctgg aatttgcgtg
     7081 catacccacc gccctggagg attggtcatc accgaacggg ctctcgcatt atgcgattta
     7141 ccagagcggg cgcgattact ggatgttgct tgcggcgcag gcgcgacggt gaattttctg
     7201 tcaagtaagg ctgggttgaa agtgacagga ctggacaggt ctggcaaagc gctctccggt
     7261 attcaaggta agccaatcct gttgaacctg gtgaggagcg atttactgaa catcccgatg
     7321 gcgccagagt attttgatgg cgttttcatg gaatgcgcgc tttccttgac gggtgaaccc
     7381 ctcaatagcc tgttggagat caggcgagtg ttgaagcccg gcggaaagat cgtccttaca
     7441 gatatctatc ttagagaagt catgaccgaa accattcagc gtgttcttgc gggcagcgct
     7501 tgcctggctg gcgccatgac ccaggacgga gttgaagagg tgatcgcggc cgcggggtta
     7561 acaaggttgg tttgggaaga ccattcacca ttgctgaaac aatggttggt tgaacatatc
     7621 tttcgcctgg gatcggtgtc cgggcttttt cagcagctca ccagggagaa tgagcaaaac
     7681 gagagggcag cgcaagcctt aagccgcgtg cgcctgggat attacctggc gatacttgaa
     7741 aaacctttgt cttttaagag ccaggaggct tatgatggat gatctgtcta aaatccgcag
     7801 gtttggcgaa cagggatttt actgcagcca ggtcatccta ttaatggggc ttgaatggca
     7861 gggaaaggaa gaccccgctc ttgtgcgcag tatgcactcg ctggcaggtg gaattggatt
     7921 tagcgggcat aactgcggcg cgttgaccgg tggcgcctgc ctgctgggat acttcgcagg
     7981 cagaggttct accagtgaag aagaggacga ccgtcttttt ctgatgctga atgaactggt
     8041 ggactggttt accaggaaat atggagtcga atatggcggg atagactgcg agaacattct
     8101 atcaggtggt aaggatcagt atgtggctcg ctgcccggga attgtgcaat ctacactgca
     8161 gaagtgcaag gagttattgg tggaatatgg ctatgagctc aatggtgagt gaaatccctg
     8221 tcgagggatg tgtgatttgt cgaacacaga gtatctgccc tgtctgttta aaccggcttg
     8281 aagcgaaacg ggttgaatac gaggatggcg tatacctgga gaagcactgc cgcgaacatg
     8341 gtttttttcg caccatcctg tggcgcggaa ggccagatta tggcagttgg aggcgcgaaa
     8401 agctccccaa tcaccccagg caccctttta cacaggtgga gagaggttgc ccttatgatt
     8461 gcgggttatg cgcagaacac cgccaggaac cctgctgtgt gctgctggag gtgaccagcc
     8521 gatgcgacct ggactgcccg gtgtgcttcg cttcttctgg agagaaagac gctactgatg
     8581 tgccgttatc cacaatctcc ttgtggtatg aacgtatgct caaggcggga ggacctttca
     8641 atattcagct ttcaggaggt gaaccctgtg ttcgtgatga cctggcgcag atcattgcca
     8701 tgggaaagaa agcgggattt tcctattttc agcttaacac caatgggctg cgcatagcca
     8761 gggagagaga ttatctcgcc agcttgaaag aggctggtct cggcgtggtt tatctgcaat
     8821 ttgatggcac aacagacgac atttaccgaa ccattcgagg aagagaaatt ctggacgcga
     8881 aaaagaaggt tatcctcaat tgccgcgcgc tcgaattggg cgttgtactg gtgccgactg
     8941 ttgtgccggg agtcaacgac aaagacttgg gaaatattgt gagattcgcc ctggagaact
     9001 acccggtcgt ccgcggtatt cattttcaac cgatcagtta ttttgggcgc tttcctgacc
     9061 agccgcggaa tgaagatcgt atcaccatcc ctgaaatcct gcgcaatttg caagaacaga
     9121 ctgaaggatt agtcaaaact gaagattttc accccaaggg cagcgagaac tcatactgtt
     9181 cttttcactg cacctacatt cttatgcctg ggggcgagct actggcggtg aaaaaccagg
     9241 cgggtaaaac ctgcggctgt cgagagcctg aggattccag taaggcttta ttacgaacca
     9301 ggtcatttgt cgccaggaac tggatatatc ccacgcctag cgctccttgt tcttgtggcg
     9361 agtcggcttc catgggagag tgggatgtca ttctggagcg ctcgaaaacg catctttttt
     9421 ctgtttcagg aatggctttt caggacgcct ggaaccttga cctggatttg ctacaagact
     9481 gctgtatcaa tgtggctgct gatgatggaa aattgattcc tttctgcgcg tacaacctca
     9541 cgaacacgct tggagagggt ttataccgcc tgtaaggcca catggaattg aagattactc
     9601 ccctacataa ctggatttca gcgcgtattg gcacccctcg ggccgcgttt gaccgccagg
     9661 cgctggagcg atggcagttt agcagcctgc tgcgaatggt cgcatatacc cggcaaaaaa
     9721 gcccttttta ccgcgagcat tttgcagggc tgggaccaat caattcattt ccggattttt
     9781 tcaggctacc gttcacaact gccgagaatt tgatggaaaa cccccattcg ttcgtatgtg
     9841 tctctcaaga gcaagtccat cgtattgtgt cattgcccac ctccggcacc acagcgaaat
     9901 caaaaagggt ttttttctct caggaagacc aggcgctgac cattgacttt tttcgaacgg
     9961 gcatgtccac gctggcaggt gcaggggaca gagtgctgat cttgctgcct ggcgagaggc
    10021 cgggcagcgt aggagacctg cttttcactg ccttgcgtga actgggctgc ttgcccacca
    10081 aatatggccc ggttgatgat gaaaggagtg tattgcaggt cattcggcaa gaaaaagtga
    10141 acatactggt tggtgcccca gttcatctgt accgggtggc ctgttttgac catgtgacta
    10201 attttctttc gcaagggcaa ataaaagcgg tattgacctc cacagatgtg ctgcctgtgg
    10261 ttgtggcgga gaggttgaag gctctctggg gatgtgaaat atttgaccac tacggcatga
    10321 ccgaaaccgg gttgggcgga ggagtcgagt gcagcgcgca ttgtggttat cacatgcgtg
    10381 aagcagacct ttacattgag gtcattgacc ccctcaccgg ggaggtacta ccagacggag
    10441 aagaaggaga ggtggtgatt accacgctta cacgcaacgc catgcccttg atacgctacc
    10501 gtaccggcga cctcagccac gtgatacccg gggattgtgc ctgtggttct tttataaaaa
    10561 gaatagcacc catcaagcgc cgctggcgtg cgggtattca gataggcgag tgtactcttt
    10621 tccaaaacga cctggatgag gttatgtttc aactgaatgg cgttggtgat tttgaagcat
    10681 ctgtctttgt ggatgaacaa ggccgagaca tgatgaggtt gtctctacgc ataatgggtg
    10741 gaaaaaggga gcgtgttcaa gaagaagccc gggaagtggt atcgcgtatc ccgggcatgg
    10801 aggaattatc atgcaatggc aggttgttaa ttgaagtggt tgatgcgctg aacccggtat
    10861 ttgccaaaag aagaattatt gacctgcgcc caaacgtaca acatcccaca caggccagcc
    10921 gataagccgc attctgttcc ctgaaaaggt atgcgcgtcg cacggtttcc aggtagatgg
    10981 tcatctctct aggccgggtg ttgccaccag gctcgtgcag cctacccggg actcataagg
    11041 aaacgggccg tttcccgccg ccgtagcgga catggtccct gcttggcctt gctccggttg
    11101 ggggttacct ggccgctgca tttcggcagc gccggtggtc tcttacacca ccttttcacc
    11161 cttgccctta cgggcggtat gtttctgtgg ccctgtccgg cgggttctcc cgcaggatcc
    11221 ccgccccggg tgttacccgg caacctgccc tgtggagtgc ggactttcct cgacgctgat
    11281 cgctcaacgc cgcgaccatc tggctggcct gatacttcca tcataccttc tcttaatccc
    11341 cccgtcaatc ttaaaaaaac gtccaggagg tttaataaga gtaaaataac agggcaagga
    11401 tgtaccagac ccttgctgga aggtagagga taaagaagag attatgcgaa aaccattggt
    11461 cgccggtaac tggaaaatga ataaaacggt tgaacaggca agtttgctgg ctcgagagct
    11521 gttaacgggt ttacagcagg tgcctgaagt tgagcgggtg ctttgcccgc catttacctc
    11581 gctgatgatc ctttcggccc aattgagcgg taccggggta ggtattggcg cacaaaatat
    11641 gcattgggca gaatcaggcg cgtacaccgg agagatatca ccacaaatgg tgcgtgagtt
    11701 ttgcgaatat gtcattatcg ggcattctga acgaagaacc tattttggcg agacggatga
    11761 gagcgtgaac cgtaagctga aatctgctct ggctttggga ctggttccca ttgtgtgtat
    11821 tggcgaaacg ctggaagaat acgaggcggg tgtaaccggc gaggtaatta cccgccaggt
    11881 acttgaaggg cttaaggata taggaacggc atcggcttca atcatcgtgg tggcttatga
    11941 accggtatgg gccattggca ccggcagagc agccaccgct gaaggagcaa acgcggtact
    12001 gcgggatcat atacgaccgg cgctggcaca gttatttggc tcagggctgg cacagaatct
    12061 gcgcatcctg tacggtggtt cggttacggt ggccaatgct accgaatttt tccgtcaacc
    12121 ggaaattgat ggcgcgctgg ttggcggtgc cagcctcaag gtagatgaat ttgtgaaaat
    12181 cgtggaagcc gcaaggccat aattaagaac agaacaacag aagacctggc tgttggctgg
    12241 gtctttttgt attttacgcg gtgccaaatt ctcgatcgcc ggcgtctccc aaaccgggaa
    12301 gaatataacc gacgttattt aagcgttcat ctaccgcagc caggtggatg ggtacctcgg
    12361 gatgtgcctt ggtcatcgca gcaatgccct cgggagcccc gataatgccc acgaacttga
    12421 tcctcttcac gccccagcgt ttcagcacct caaccgtcgc tgtggctgaa cctccagtcg
    12481 caagcatggg gtctaagatc aggcacacgg agacggttgg ctcagttggc aatttgttgt
    12541 aatactcgac aggcttgagt gtgttctcat cgcggtaaat gccgatatgc cagacctcgg
    12601 cgccgggcat tagatcccaa aaaccttcca ccatccccag tccggcgcgt aagataggga
    12661 tgagaccaat cttgtctttt aaaatcttcc cagaagccgt ggcgagagga gtggttacct
    12721 ctatatcttc catcaccaag tcagcggtag cttcgtaagc cagcaagccg gcaatctcac
    12781 gcaccagctc gcgaaacttc ttgggttcgg tgcgggcatc acgtaatttt gctaatttat
    12841 gagcgaccag gggatgagcg gacacatgaa ggttggacat agcggctcct tgaaaattta
    12901 tatcattata gcgccaaata aaaacagcgt tcaacaaaat ggctcagttg atcccttgca
    12961 ggcaggggag cgtgtataat ggggtttatg acagcagacg aacgcatcgt gaaccctgtg
    13021 ccaaaggtgg acgatcgggt agataacgtg ttgcgccctt ccaaattgaa ggaactcatc
    13081 ggccaggacc aggtacgcca aaatctggag attttaattg cggctgcgaa agggcggaag
    13141 gaatgcctgg accacgtgct gttttacggc cctcctgggc tgggaaaaac taccctggcg
    13201 tacattctgg caaatgaaat gggggtcaac atcaaggtca cttcaggtcc ggctattgag
    13261 cgcaccggtg acctggcagc gatcctgacc aacttgaggg aaggtgacat cctgtttgtg
    13321 gatgagatac accggctggg tcgctctatt gaagaggttc tttacccggc gatggaagat
    13381 ttctcattgg atatcatcat tggcaaagga ccttctgcac gctcgctgcg ccttaaattg
    13441 ccgcgcttta ccaccattgg cgccaccacg cggctggcgt tggtcaccgc gccgctgcgc
    13501 gcgcgctttg gcgctgttta ccgccttgat tactacgacc tgacagccat gcgtaagatc
    13561 gtagaacgcg ctgcgagttt gttgaatgtg accagtgatg ataccggaat agaagaaatc
    13621 gcccgccggg cgcgaggaac gcccagggtg gcgctgcgct tgttgcgccg cgtgcgcgac
    13681 ttcacccagg tgcgtgcaaa tggtctcatt acccgagaaa cggccatcaa tgcgctggat
    13741 ttgctggcgg tcgacgcgat ggggttggat gatgtggatc gcagagtatt aaagaccatt
    13801 attgaaaaat ataatggcgg tcctgtaggc ctggcaacaa tcgccgcttc gataagcgaa
    13861 gaggcagaca caatcatgga tgtggttgag ccatatttac ttcaactggg atatatcgac
    13921 cgcactcccc agggtcgagt tgccacgccg ctggcttacg aacacttgca attgccttat
    13981 aaccagaagg ataaccagcc gcggctgatt ccttgagcat gcagaccgct gattttgatt
    14041 atcacctgcc gccagagtac attgcccaaa cgccaatcga accgcgacac gcgtcacgtt
    14101 tgttggtgtt acggcgttct tctgccaccc tggaagacag ccacttttgc cagctgcctg
    14161 attttctaaa tcctggcgac ctcctggtga tcaatcaaac aaaggtgata ccggcgcgtt
    14221 tacacgcccg caagaccagc ggcggcaaag tggaattgtt gctgctcaaa cagcgggagc
    14281 cgggtttatg ggaagccctg gcgggaggta aaggattgcg caaaggggtg cgcgttcagc
    14341 tggagaatgg accttatgtg gacattgaag aggaactgac gggaagcgaa aggttgattc
    14401 gcttttctga gccagtggag caattactgc caaccatcgg ggagatgccg ttaccgcctt
    14461 atatccatga gaaattgctg agacctgacc gctaccagac ggtgtacgca actgaaaccg
    14521 gttcagcagc tgcgccgacc gctgggctgc attttacccc ctggttgatg gatgaaattg
    14581 aaagaaaagg ggttcgtatt gccaaagtga cgctgcatgt cgggttggat acttttgccc
    14641 cggtaacaga gttggacccg gttgaacaca aaatacacac ggaatggtgc gagctcaccg
    14701 aagaagcggc gctgctgatc aaccgcacga aagcatctgg tgggagagtg gtggcagtgg
    14761 gaaccacgag cgtgcgcacg cttgagagcg ccgcggctac cagcaaccag ccctcttggg
    14821 tagcgcccta ccgtggacaa acagggcttt tcatccttcc gggatttgaa ttcaaagtgg
    14881 tagacgcgct ggtcacgaac tttcatttgc caaaatccac tctgctgatg ctggtgagcg
    14941 ccttcgcagg gcgggagcga gtgctggatg cctaccaggc ggcgatggag cgcgggtacc
    15001 gcttctattc ctttggcgac gccatgctca ttctttagga gactcattga cctgtccgcg
    15061 cagcctgaaa ttattggata accaaatcat cgcctgcaga acctgtccac ggctggttgc
    15121 atggcgggaa aaaattggtc aggagaaacg gcgcgcctac ctggggcaaa cttactgggc
    15181 aaaaccatta ccaggttttg gcgaccccct ggcgcgtgta ctggtggtag gcctggcgcc
    15241 gggagcgcat ggaggaaacc gcaccggacg aatgtttaca ggtgacgatt caggcgactt
    15301 tcttttctca gcgttgcacc gggcagggtt tgccaaccaa cctgaagcca aggcattgaa
    15361 cgacggctta atgctcaatg atgtttacat cacagcggtt tgccgttgtg tgccaccgga
    15421 taataagccc ttgccagctg agatctcaaa ttgccttcca tacctgcagt gcgagtttga
    15481 attactggag aaagtagaag gcgtggttgt cctgggcagg atagcgtttg acacagtcag
    15541 taaattgctt tatcaccagc gcaaattggc cacctcggcg atttttgggc atggggtcct
    15601 gcagccatcc actgatggtg ccccctggtt gttgtgctcc tatcacccca gccggcataa
    15661 cacccagacc ggcaggttaa ctaccaccat gttcgatcag atctgggaac aggtgaaccg
    15721 gctgttggga aaagatgagt tactttcttg tcaggaacca ggctcgtcct gactggcttc
    15781 ctaacgggga gatgggatcc aggctgctga ctttatcaac cacgtgaagc cccgcatatt
    15841 ccagacagga ttcgatctca gcgattgaaa aaccgcgttc gatgtgcttt tcttcaaatt
    15901 tttcccactt atcatccact ttgacaaacc aggtaatctt tatacagccc aggttctttt
    15961 cgaaatcata acaagtgcga tgaagttcga gcaagtcctt ggtttcttgt cgaacataac
    16021 agcggttctt ttgccactcc accgctaacc cataaattgt gttcatatca aaaaggaacc
    16081 aaccattggg ttttaaggag cgcgcaatgc tgttgaaggt ggattgcagg tcgttgttgg
    16141 aaatgaggta gttgagacta tcaaaccagc aagtcgccag gtcaaattcg ttgtgatagt
    16201 caatgaagcg catatcctgt ttttcaaatt tcacatcgac ctggtcattt tttgcccgat
    16261 gttttgccag acgcagcatt tcttcagatt ggtccacgcc ggtgactgac cagccattct
    16321 ttgccatttc cacgcaaaag ctgccctcac cgcaggcgac atccagtaat ttctttctgt
    16381 tattcgtgag tattttgtag tgcttcatca cccctggaag aatagcagcc atccgttgtg
    16441 agaggttcgg ataatcaccg ctcgcgtaga cactggcaaa actgtcatat agtttcatct
    16501 ctctccttgt ggggcctccc aaactggcag gttcatcaat tagtaccaca cttggcggga
    16561 attgaaaatg ttacggattc tggatgtgat tacaactctg caaacaaaaa gacagcggtg
    16621 gaggccgctg tctttgaatg aatgaaaaaa ttaataggct ttgagcaccg accacccagc
    16681 gtaagcggcg gtttcaccca gttcagcctc gatgcgcaat aactggttgt attttgccac
    16741 acggtctgag cgggcggggg cgccggtttt aatctgccca gtgttgagcg cgacagccag
    16801 gtcagcaatg gtagaatctt cggtctcacc tgagcggtga gaggtaaccg cccgccagcc
    16861 ggcacggtgg cacgcttcga cggcttcgat tgtttcggtc agcgagccga tctggtttac
    16921 tttgacgagc agggcgttgc aggcctcttc ctttatagcg cgccttaccc gtgacgtatt
    16981 ggtaaccagc aggtcatctc ccacaatttg caccttgtga ccgatttctt tggtcaacaa
    17041 cttccatgaa tcccaatcat cctgcgccag cccatcttca atagacacga tgggatactg
    17101 tctgacccag ttcaaccaga actccaccat ctgttcgcca cttaattttt tgccctcctt
    17161 acgcaggtta tacgttttgg tttcttcctc atagaattct gatgcggcag ggtcgagcgc
    17221 gatggccact tcttcaccag ccttgtaacc ggccttttca atggcttcaa ggataacctc
    17281 aaccgcctcg ctgttggctt tgagagctgg cgcgtagccg ccctcatctc caaccagggt
    17341 gggatatcca cgagatttca acacaccttt aagcgcctgg taaatttcat ccccgcaacg
    17401 aaggccttca gcaaaagatg gcgcccccag cggcatcacc atgaactcct gcgcatcggt
    17461 agattgccat ccagtgtgag cgccgccatt cagaatgttc atcatcggta ctggcagtac
    17521 atgcgcgtat accccaccaa tatagcggta gagcggcaga tcgagtgacg ctgctgcagc
    17581 tttggcgacc gccaggctca cacccaggat ggcattggct cccaggcgag atttattggg
    17641 cgtaccatcc aattcaatga gcatctggtc gatggctttc tggtcgatgg catcccacca
    17701 gatcaggttt tcagcgattt caccgttcac atgctcaacc gcttgcagca cgcccttgcc
    17761 gttatagcgg tttttatcgc cgtcacgcat ttcaagcgct tcgtgaatcc ctgttgacgc
    17821 gccggaggga accgcagccg tgcctattga cccatcttcc agtatcaccc tcacttctac
    17881 agtggggttc ccgcgtgaat cgagtatctc taatgcttca actgatgcga tttccatttt
    17941 ttatcctttt ccctttttca ggtgtggtta attgattttg agcccaaacg gctgtaagta
    18001 taacgaaaaa tctctatttg tgggttaccc accgaggagt tgccgaagtt ttttaaccac
    18061 ccggagtgtc gattgaccgt cattgtatgt gcatatttct tcaacgatct tcttgcgccc
    18121 ttcggttctt tcagctggct tttcgaagcc atcattgacc gcgcggatga aatcgggcct
    18181 ggagtaaacc aggtccaacc ccccggaatt ggtgatggga atgtagtgtt cgcggatata
    18241 cagttccttc agcacgcggt catacttccg gctgctgccg gggtcatagg cgggtccgat
    18301 ctgagggcgg tcgaatacag ccccatccac cgtgagggta gaagaagagt tgatctgcac
    18361 cgcgcaatgc gcaagtgtgg aacagagttg ttcgatatcg gcatgggtga tgttgatctg
    18421 cccacggctt tcattggctt gccagctttg gtcagtaaag gtgtttttta ccccgctcaa
    18481 cacctcttgc cagcggctgg cgatatcccc tgggtgacgg cggaacagaa tgataggatg
    18541 tgcaggaacc tctccattcg aaatggcgtc atccagttgc tttaagaaga gcggctcatt
    18601 gggaacgatt ttatatgtac cgcccccgaa aagaataacc ggtcgcgcag atgggagccc
    18661 gagcctggaa cgccattttt cttccggtat gacgtttgca gaatcgtagt aaaaatcaaa
    18721 ctgcggagga ccggaaatgg tgatctgttc aggagtgata tcagggtatg cgcgcaacag
    18781 ttctgcgtgg ttgtagcggt tccaaaccag gtaatgctca aaagaaacag gcaggcgggt
    18841 acgactggtg acgttatcaa acgaaaggat ggaggtaatt cgcaaaacgc cgcgcttttg
    18901 cgcgcaacgc aataacggtt cttctttgtt gacaaaagga gtaatcgaaa agaccgcatc
    18961 gggtttggac tggtcaagaa tggcatcaaa ttcctggtaa ttcgtctccg accagaacag
    19021 ggattcttcc tgctgcagga ggtgcgggat gcgcgccggg agggctgttt tgattctatc
    19081 cttcagaatt tgaacctcgt agttcaccct ttcccgcaaa cccagcagag aggcttcacg
    19141 gcggatatca atctcagtgg tgttggtctg ccgcacctgt ttatgccaga agtttacaag
    19201 ttcccgggtt tctttgaatt gggggctgaa ggtgaagttg ggaacatgaa gaatttcagc
    19261 gccagcctgg ctgaattcat tttcaagcgc cgggtcttgc caacttaaag ccacgatcgg
    19321 ttgagcgaat tcggtgagcg ccgtcaacat accggttctg agaatgtagc gcacggaaaa
    19381 gtgggtgaag acagggatta ataacctggg tttgggcata ccgactccgg gacttggctt
    19441 tgttaccttg tccatgtaaa ttgaataacc tggatattat atccgcttat gcgaggatta
    19501 acgtttgtac aacagcggcg ggtcgcgata ggccactccg gctttcacag ccgcgacgcg
    19561 catgaaagcg aggaaaagcg ggtgcggtcg ggtaggtcgg gagaggaatt ccgggtgaaa
    19621 ctgcgtgccc agcataaatg gatgatcttt cagttccaca atttcgacca ggtttttatc
    19681 aggcgaaaga cccgaaaaaa ccatgccgcg ttgcgccatc cgttctcggt aggcgttatt
    19741 gaattcaaag cggtggcggt ggcgttcttc gacctttttc gcaccatacg cattggcagc
    19801 gatggtgcct ggctgcagga tacagggata gagccccagg cgcatggtgc cgccaacatc
    19861 cttaatgttc tgctggtcag gcatcagatc gattacgggg tgcggggtgc tcaggtcaaa
    19921 ttcggcagag ttcacctggt cagaacccag agcgtacctg ccgaactcca cgcacattaa
    19981 ctgcatgccc aggcaaagcc caaagtacgg aatcaagttt acacgagcgt aattggcagc
    20041 ccaaatcttc ccttcaatgc cccggctgcc gaatcctccc ggcacaatga ttccgtgggc
    20101 gtcttccaga gcatttacat gttttgcttt ttcaagatca gccgaatgaa cccacctcaa
    20161 atccagttcc agacccagag agagcgcggc gtgttttaag gcttcacgca cactcatgta
    20221 ggcgtcatga agctctacgt acttacccac cagggcgacg gaaaccctgg gtttttcacg
    20281 cgctgtttcg ctgaccagtt tttcccagtc actcatatcc ggctgttgtt tggaatccag
    20341 gttcaggcgg tgcagcaggt attcgccgac tttttctttt tccagcagca gagggatttc
    20401 gtaaaggatg ggcgaagtga ccattgggat cacggcgcgt ttttcaacat cacaaaactg
    20461 cgcgatcttt tcccgcaaat tttcagcaac cgggtgatca ctgcgggcaa cgatcatatc
    20521 tggggcgata ccaatggagc gcaattcagc cacggagtgc tgggtgggct tggttttaat
    20581 ttcatcagtg gcaccaatat aaggcagcca ggtaacgtga acattgacgg tgttttctct
    20641 tcccaggtct gaacgcagct ggcgaagagc ttctaaaaag ggtaggcttt caatgtcgcc
    20701 cacggtgccg ccaacttcta caatgacaat ttcggcgctg gttgatttgg acaccaggtt
    20761 gatccggcgt ttgatctcgt tggtgatgtg agggatgacc tggatcgtgc cacccaggta
    20821 atcaccgcgg cgttctttcg cgatgacttc agcataaacc tgaccagtgg tgatgttgca
    20881 cacccggttt aggcggatat caataaagcg ttcgtaatga cccaggtcga ggtcagtttc
    20941 agccccgtca tccagtacat aaacttcgcc atgctgatat gggctcatcg ttccagggtc
    21001 gacattaata tacgggtcga gtttttgaac ggcgacttta tacccgcgtt ctttcaggat
    21061 caatccgatg gctgcggcgg tcacgccttt tcccactgaa ctcaccactc cgccggtgac
    21121 aaagatatat tttgtggtca tggtctgctc ctgaatgaga aatataaaag aagcgaggag
    21181 ggcggtttat ccttgtcctc ctcggaactc acttgatgta acccaatatc tcgcgtgaaa
    21241 tatcagcatt tgtcttttct caataaaagt gtatcacaac tcgattttcc tgacaaaaga
    21301 aaaaccatcc agaaggatgg tttttttgaa tcagttggtt tgaactttgt tttccagcgt
    21361 gcgggcgagg atttcatcct gctcggtgga gccgggtttc gtcctatgga cttcctgttt
    21421 ctttttcttt tgtttggctt tttccattgc ggcacgcatc gccagctcca tataggtggg
    21481 ttcaggctct acagtctcgt tcttcacttc gagaaccccg gagctttcaa cttctgattt
    21541 tcgacgaggt ttcctggcgg atttcttctc gcgccgcgct ccctctgcgc cggaagcagc
    21601 gggagctggt ttttcttcaa cgaccttttc aggctctggt tgaatggcct tcatacttag
    21661 tttgatctgt ttcttgcgcc gattgacctc gataatttgg gcttcgacct catcgccttc
    21721 ctgtaccaca tcagatggca gcttcacata accatgcgcc atctcgctga tgtggacgag
    21781 cccgggtctt tcagcgccaa tttccacgaa ggctccgaac ttctccaggc gaacgacctt
    21841 tcctttgaca gtttcgccca ccttgatttc acgccactca agatctaagg gcttgaacat
    21901 ggtcatttca atacgttctt cgctatcttt tcgggtgagc tttttgaccc acacgtcgat
    21961 ttcttctccc tctttgagga gctcgtcgac cttcttgttc tgtgtgttat ctggagcaat
    22021 gatctgcgaa acgtggagca aagcaggttt gccgatgccg atatccaata cagcgccggc
    22081 caggctcacc ttgattaccc gtgctttgaa cggcattttt ggttcaagtt gctgattgtc
    22141 ttgagtttct gcgttcattt ccatcaatat tctctttcat ctcggtagtt agcgaggaaa
    22201 aattataccc cctgcaggga ggaatgtcaa ttttttctca ctgttttgag aaaccaattt
    22261 acttgttcgc gttcaacagc aggtcaatcg ccttttccag ttgaggatca agttctgctt
    22321 ctatatcgtt ctctgtgcgc tccacaatgt aatcaggtgt gagcccaatt ccgtggatga
    22381 gcctgccctt aggggtcaac caacgagcga tggtcacttt tacggcaccc tgttcatcag
    22441 cgaggggcac ccagttttgc acagatcctt tgccgtaggt ggttgtgccg acaatttttc
    22501 cgcgctgata gtcttgtatc gcgcccgcgg tgatttcaga ggcggaagcg gaatactggt
    22561 taaccagtac caccagtggt atctcggtag ccaacccgcc tggatgcgct tcgaatgtga
    22621 cctctttacc atcgccgaac ctttctatca tgatcacccc tgacccaatg aactgcgaga
    22681 tgatatcgat ggcggcgtcg acataaccac cgctgttatc gcgcaggtct aagatcagcc
    22741 cgaccgggtg ttgtgccaac aatcctgcca gcgccgtttc catttcttgt gcgctttcgc
    22801 ttccgaatgt gttcagggcg agataaccga tgccctggtc aagcatgcgg ctttcaacac
    22861 tggaaaccgt gatctgtgca cgcacgatgg tgaaatcaag gatttcttct ggagcatggc
    22921 gctgaacggt taagactacg gtggtgcctt caggtccttt cacggtttgg agtaccgcgc
    22981 ttgggtcctt accaatcaca ctttgaccat caaccccgat gatctcatcg cccggcttca
    23041 accccgcggc ttcggcagga gaaccagaca tggagcggat gatggtaagg tactcaccgg
    23101 ctgtgtccac ccaggcgcca atccctccat acgaaccgtc caaagttgtg gtcgcctggt
    23161 cgtaatcttc cggggtcatg tacccggtat gaatgtcacc gagcgaattg agcatgcctt
    23221 gaatggcgcc gcgcatcagt tgaaggtcgt ctaccggttg atcaacatac tgctcgtgaa
    23281 tgatgtccca cgcttcccag aaaggttgga acaattttct gagatccgcg ggtgtagatg
    23341 aaggcggtgt tgaatcaggt aaggggggcg tttctgcagg ctttgttgta tcaggcgcga
    23401 gcgtctcgag cggagaacca gagggaatca accagccaag gataacaccg ccggaaaagg
    23461 caccggctaa caacacaagt gaaatcataa tgaccaggat gatcctaccg gtgtgattgg
    23521 tgttcatttt tttactcctg cttcatggtt accattcttt gaatgttcga cgggataagt
    23581 tttggcctta agatgctgaa cgcgctttga aagttcgtgt attttcttgt tttcttcggc
    23641 gaaagggtcg cgcagcgtgt cgcctgcttt tttcactgtg tttgcccaat ttgagcgctg
    23701 ccctttctct ttcaacctgg aaaataacga gatgaacaaa ctgatgatta acatgaccgt
    23761 aaaaacaatc aggatccaac ccaggagtgg tattggtttt gccaaggttc agcgctccat
    23821 tgtcctgtgc gaagagggga tcacatcagg caggtcaagg agagatagta tcgctgcgtc
    23881 agcaaattcg cacctgtttt ccgttcctac ctgtatcgtg taaaagccca tttcatgggc
    23941 ggtctttaaa tttcgcggcg agtcatcaat gaatacacag tgttcagggt cttcaatttg
    24001 aatgatttcc atggctttct ggtatgcctg gggcagcggt ttacaatacg gtgatatctg
    24061 aagaacatcg acaatctcag ggaagaaatc cacaagtccc aggacctcta aaacccgctc
    24121 agcatgcctt tggttggcgt tggtaaagat aaccttacgg ctgtcatagg cggaaatcgt
    24181 ttcaataagc gcttcatctt tgcgcaagaa ttgcgtaaga gggatatcgt gcacgtaatc
    24241 caggaattca cattcgtcga tgtgataaag ggtctttaac ccgcgtaacg tggtgccata
    24301 ttcgtgaaac agcccattgc ggatttgttt aacctcgttt gcggccactc cgagtgtttt
    24361 tatgatgtac gaatccatac gatcgccgat ggcatcccac acgccactag tagaagggta
    24421 aagggtatca tccagatcga agagcattac agcaaaactc atagcggtta gtataccctc
    24481 aaattgattc cccggtctgg attaaccccc gataggggtc agttataatt agaccgatga
    24541 gcatacaggt gctttctgaa gaactggctt cacaaattgc ggctggagaa gtggtggaaa
    24601 gaccggcttc agtggtgaaa gaattgctgg agaacgccat tgatgccggc gcgtcgcgca
    24661 tttcaattcg ggtagaagac gccggtaaaa aaaagataga agtaagtgat gatggaactg
    24721 gcatccctat cgatgaatta ccactggccg tctcaaggca cgccaccagt aaaatcaata
    24781 ccgcggagga tctttttcat attcgcacgt tgggcttccg tggagaggcg ttggcatcta
    24841 tcggctctgt ctcccgcctg atggtcttgt cgcgtaacgc agattcccag gttggcgcgc
    24901 gcatcatggt tgaaggaggg agaatcgtca gcaaggaaat gactggcagc gtaaccggta
    24961 caatcgtgcg cgtcagcgac ctgttttaca acactcccgc cagattgaaa tttctcaagc
    25021 gggatgtgac ggaaagacag cagatcgata ccctggtgag caggtatgcg atggcgtatg
    25081 ccggcaaagc gattcattta gagcatgatg gcaaagcggt attacggact tcggggaatg
    25141 gtgagcggcg ggaagtatta gcgcagctgt atggaattga actggcgagg cagcttctag
    25201 aagtggtctt tgaagaagaa gggatgaaga tcgagggtta tatcagcccc atctcgatca
    25261 gccgttcaaa ccgtaaagat atgacctttt ttattaatgg gcgctgggtt caggacgtca
    25321 ccctggtgag cgcgttgttg aaggcttatc aaacctacct gatggttgga cgatacccgc
    25381 tttccatttt atttattgaa attcctccag aagcggtgga tgtaaatgtg cacccggcca
    25441 aggctgaggt gcgctttaga gagccagacc gtgtatttac cgctgtgcaa cgcgcggcca
    25501 ggagagcgtt gatggcttac gttcctatcc cgccactggc ggctgcttcc tggagcagcc
    25561 gggggtggaa cccttcgcct gaactggatg gcacgtggat gaatgccaga cccgtgcagc
    25621 cgcagagtac tggtcaaccc caaccgatgg ctgaaacacc tttatcatca tcggtagaac
    25681 cagcgccttt tgcagcaggg ataccattgc tgcgtttggt cggccagatt ggcgccactt
    25741 acctggtggc agaaggccca gatgggcttt acctgattga ccagcacgca gcgcatgaac
    25801 gtgtgctata tgaaaaaatg cagcgacaaa aagagaacat tcctacgcaa cctttgcttg
    25861 aaccattgct gattcagtta cctccccaat ccgctcaaat catctgtgat aaccttcagg
    25921 tgttaaggaa gattggtttt catcttgaag agttcgggcg caattccttt cggctgagcg
    25981 cgattcccgc tttgttcacc caaaaagatc cccgggcagc catcaatagc gtggtggaag
    26041 attttgagga agatgaagaa cctctcaagg aggtgcacga agacaggctc atcgcgcgaa
    26101 tttgtaaacg catggcagtt aaaggcggct ccatcttgtc caccgaggag cagcaggcgt
    26161 tgttgcgcga cctcgagagt tgttcttctc cgcgcacctg cccgcatggt cgaccgacca
    26221 tgattcacct ttcagttgag ctgcttgaaa gacagttcgg acgccgggga gcccgttccg
    26281 ctttttaacc acaacgctgg ttgagcaatg ctaccatcgc cgggatgcat tccgccacat
    26341 ctgaacgcat caccaactcc gcgtatggat caatggaagt ggcagaatga ttaaccagga
    26401 taatcttgca tccattgcgg cacgccttga agggcagtag cgcggcggga gtaacctcca
    26461 acgaagaacc cgccaccaac aacaaatcgg ctagcgcgat ttcattttca gccttcagcc
    26521 agtcatccgc cgcgagctct tcttcataaa gggtaatatc agggcgaagg acagtgccac
    26581 aggtagcgca aagcgggatt tcacccgaga ggattgacgt gacgactgcg gcgaaatcaa
    26641 caccgtgttc cttgtttgcg gggcagtaaa aacgatgaat tgaaccatgc agttcaacta
    26701 cgttgaccga gccggctttt tgatggagtt catcaatatt ttgggtgatg acagcctgta
    26761 atttgccggc tttttccagc gtagcaaggg cgaggtgggc atcattcgca tgggcactat
    26821 aagagacccg cagcaagggg cgcagccagt tataaaaatt tcgcggcgat tggcggaatg
    26881 ccgtggcaga ggcgactttc attgggtcat cacgctgcca caaacccgtc cccgcgctgc
    26941 gaaaatccgg aatccctgaa ggagtggaaa tacccgcgcc ggtgaacgcc acaaaatatt
    27001 ttgaactggc gatcaaatca gcagccctgc gcagttcgtg ctcaactggt gtggtcacaa
    27061 ttgaaaccgc tggcaaatcg cgcgggagag ctcattgacc tgctgagaaa acaacccttc
    27121 aggttggata tgatacagcg ggatatattt ttgattcgct tgaaaaacct gctctggaac
    27181 aggtggaatc atgaacttga tcgtggtacc tggcagtaac ttggttatct gttcagcagt
    27241 aaggcccgcc tcagaacgga tgtgattata gaagaccgtg tcgatgtacc ggtttttctt
    27301 gggtacctca aactgcaatg ttccaagcaa ttttctggta cgtaacaccg ttgcggggaa
    27361 tgcgtctgtc agcacgatca gctggtcaca aagcttatgg accttgctga aacctgccag
    27421 gtagggattg ccgatatcca ataccaacac aggaattgag gaattcaatg atttgatgat
    27481 ggcgagggtt ttttctggca ggtcaggcag gctgattttt tgactctttt ctgaagccat
    27541 cagcaggcgg acattaaatg aggtatgcac ggctaatttt tcaaccgctg ctgctgtgat
    27601 ctcgtcaact ggcagtgcga gaagcttctc caacccatag gaattggcaa tatccagttc
    27661 gaccccccaa ctgccctgac ctggcttcag ctccacggct accacctctt tgctggtcat
    27721 gccactcagg ctgacagcca ggttcaatac cagtgttgat acgccaagcc cgcctttgca
    27781 tccaagtacc ccgatgatac aacctgaacc gctttcttcg ggcgtatttt ttattttttt
    27841 catacgatcg agcaaggctt tgacatgggc gttgagttca gcggggtgca ctggtttggt
    27901 caggtaatca tctgcgccgg cgccgtaacc agcgattttg tcacccatct ggtttttcgc
    27961 ggtaaacatc aggatgggga tatgggtgca cagggggttt ttccgcagtt cgcgggcaat
    28021 ctgatagcca tccatgcccg gcatcacaac atccagtatg atcaaagaag gttttacttt
    28081 gaccgcagtc tccagcgcaa gctcgccggt gtttgcagca gcaatttcgt agccctgacg
    28141 ctgcaacatc atcccgacga gacgcagtgt ctccatatca tcatcaacga tgagaatttt
    28201 ttccggcatg ccgaccaccc ctcctgaact tataagccag tctagcatat tccctgacaa
    28261 gattcaagtg attat
//
LOCUS       DAOSPM010000011        27458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1752_length_27458_cov_15.355253, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000011 DAOSPM010000000
VERSION     DAOSPM010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 27458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27458
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1752_length_27458_cov_15.355253"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLW25_02115"
     CDS             <1..396
                     /locus_tag="PLW25_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HOS53316.1"
                     /translation="KHLREELGMAIIWITHDLGVVASLAHRVMVMYGGYIIEEANVND
                     LFADPRHPYTVGLLGSLPKIHEDVHKKLFSIEGMPPVLYKKPNACPFAPRCNWVIERC
                     WQENPTLKTVMDGHSVACWVDIRNGSKKR"
     gene            393..1373
                     /locus_tag="PLW25_02120"
     CDS             393..1373
                     /locus_tag="PLW25_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS53317.1"
                     /translation="MTTNNNVLIKVENLVMHFPIYRGVIRRQVGVVHAVDGISFTIQR
                     GETLGLVGESGCGKSTTGRAILQLYKPTAGNVYYKDTNLVKLRPEPMRLMRREMQMIF
                     QDPYASLNPRMTVRDIIGEPLMAFGENNSKQIDERVASLLDMVQLNPDFSSRYPHEFS
                     GGQRQRIGVARALALNPSFIVCDEPISALDVSIQAQVVNLLEELQQELNLTYLFIAHD
                     LSMVRHISNRVAVMYLGTFVELADRTTLYRKPLHPYTQALLSAIPVADPTYNEQRRRI
                     ILEGDVPSPVNPPSGCRFRTRCPLADTICAEQSPEWREVEPGHFAACHKI"
     gene            1483..3507
                     /locus_tag="PLW25_02125"
     CDS             1483..3507
                     /locus_tag="PLW25_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS53318.1"
                     /translation="MRKVYVLLALLMVVSLILTACAQPTKPPVTEPPAGEEPPVAEPG
                     EVVEVVFNPYLGSNKLDGNGIPPTFFDDVHVRKAMAYCFDYQTVIDDVYMGEAVQSLT
                     LSLPGMPGYSNDTEHYTYDLAKCEEEFKLADVDKDGIPAGEDPDDIWEMGFRFQMLYN
                     TGNTTREIYTEIVNSNLSKVNKKFVVETLGLPWPSYLAAQRAHKVPMMTGGWLEDIHD
                     PHNWYQPYTTGTYGGRQNLPEDLRNQFKALLDKGVGLVKFEDRAEVYKEFNKLYYDQA
                     VGIPLVLSTSHAFEQKWVQGRVMNPIFSSIYYYPISKAAGAKNPDVFTVMTTGSPDTF
                     DPALSYDTASGEIVQNVYETLVFYDGVSTSEYVPQLASEYSVSDDGTVWTFKIREGVK
                     FHEGGDLTASDVAYSFQRGLLQGGYSSPQWMLAEPFLGVGNDDITMIVDEGASADDRE
                     ALMANDPAVLKAACETVMSKIVADDATGTVTMTLAQPWGPFLGTIANGWGSIMDKEWV
                     IEKGGWDGSCDTWQNYYGMVSADDPFTTIANGTGPFKLDYWTQGEEVSLLRNDNYWGT
                     PAALKQVLIKDVPEFGTRFAALKAGDADVIAVPRENATQVDPMVSVAKFYDAETNKYL
                     DDQNICTIDTTILGKGRFEVCAEANDRPLRLYYGLPALSQDVLLFTFLVE"
     gene            3641..4705
                     /locus_tag="PLW25_02130"
     CDS             3641..4705
                     /locus_tag="PLW25_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS53319.1"
                     /translation="MTNYIIRRLLLVPVLLFGITLIIFGMLQFLGPVERSALYVKDFP
                     KNDKQIQSVIRHYGLDKPFYQQYWIWLNGVIDPVTGERSGGILHGDFGYSRSQSQPVA
                     NLITNRFPNTLDLALWTVFPMLSVGIWLGVQAAVHQNGVIDQAARIFSIVGTSFPVFV
                     FGLLILMFFYAELGWFPPGKNSNWVTQEIMQGNFHQYTKLLTIDALLNGRLDILWDAL
                     RHMVLPILTLSYITWATFVRVTRSSMLETLRMDYVVTARAKGLKEKDVINKHARPNAM
                     LTIATMAGMSVVGLLGGVVITETVFNYPGIGSAAAEAAQQLDVVTTLGFAIFNGLILI
                     VANLIVDVLYGVIDPRVRLD"
     gene            4716..5756
                     /locus_tag="PLW25_02135"
     CDS             4716..5756
                     /locus_tag="PLW25_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS53320.1"
                     /translation="MTETPRPTGDNTLLKEVVPDRKISRAERLLGPENYRILKGLLKT
                     PASIIGFILIGIFIFVAVFAPLIAPPVNDDPFKIPRDGFSGQPRPPGTEWKQNVPEVP
                     FWYKPLTGKDEWVHLMGTAQGQYDIFYSVIWGSRTAFRTGLIVVLATVMIGIILGSVS
                     AYYGKWVDQLIMRIVDVFMTVPYIMMALIMAAVLTPVIGRSLVPALLAMITFGWMGYA
                     RIIRSDILSVKQRDFILAARVCGVKDGRILFKHILPNAIFPTLVLASLGIGDVVLSFA
                     ALSFLGIGTPMGYADWGQVLSFARNWITNLADYWYIVVYPGLTLVFFVMGWNLVGDAL
                     RDVLDPKMRGRS"
     gene            5847..7814
                     /locus_tag="PLW25_02140"
     CDS             5847..7814
                     /locus_tag="PLW25_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS53321.1"
                     /translation="MRMRTRLTSIIALLSVMLAALFLLSACHKEQIAGNPPVNTPTVE
                     PPTPTSPPPKTLVVCLANEPGDLYIYGDTSRTKWSVLEALYDGPVDTLNYEPSAVILA
                     SLPSQENGAVSLRSVAVSEGDPVANVNGDIVALKKGTRVFPAGCTSPGCAVEWDGTTA
                     LELTQMVVNFTLLDGVTWSDGEALKAQDSVFSFSVSADEATRVLKTNIERTFSYTALD
                     DHTVEWIGQPGYLTLNPAAFFWSPLPEHALAGLTPTQMNEDPLTTRQPLGWGAYKMDE
                     WVSGDHIRLVKNSHYFRAAEGLPYFDVLVYRFIPSTPEVDLSMMLTGECDIIDTSAGL
                     ESQILRVRELEGQGKLKAYFGMGPEWEGLYFGIKPATYDDVYNPYEDRQDFFGDVRVR
                     QAIAYCVDREYIQTYITLNQSVIPSTFLPPNHPFAAAETRSYTHNPQLGIQLLEEAGW
                     QDSDGNHSTPRVAADIKNIFYNTELSLNYYVTESELHAAVTEAVVKSLGECGIKVTPT
                     FLSVGEMFAPGPEGRIFGRNFDLAELAWSTGRQPPCFLFSSSEIPSAKNKWLGTRYGG
                     VNLSGYASSEYDAACEQALSAGLDRDLLTAQSANAQQILMEDLPALPLFFHVKAMVSR
                     PDLCGLQLDVSARSPLKNIEALTLGETCPGK"
     gene            7885..7955
                     /locus_tag="PLW25_02145"
     tRNA            7885..7955
                     /locus_tag="PLW25_02145"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7917..7919,aa:Gln,seq:ttg)
     gene            8107..9450
                     /gene="glmU"
                     /locus_tag="PLW25_02150"
     CDS             8107..9450
                     /gene="glmU"
                     /locus_tag="PLW25_02150"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOS53322.1"
                     /translation="MNLSTIILAAGQGTRMNSTLPKVLHPLNGKLLVKYSIDLADALS
                     SSRTVVVIGNGAEQVRSALGESVAYALQPQRLGTANAVQAAEGLLADASGLVLVISAD
                     MPLLTADTLQRLVTTQSQNTGPMALLTVRSANPRGFGRILRGVDGGVTGIVEEAQATA
                     EQLKIDELNVGAYCFNSSWLWQALRKIQLSPKGEYYLTDIVAVAVAEGSPVSSLTVTD
                     EEETLGINNRVHLAEAERVIRRRINQAHMLAGVSMTDPELVYIEESVKIGRDTILLPN
                     TWLSGSTIIGEGCEIGPNTFINDSTIGNHCHLLASVVEGAVVEDEVGMGPFCHLRKGA
                     HLGRGVHMGNFGEVKDSYLAAGVKMGHFSYIGNATIGENVNIGAGTITCNFDGVNKNP
                     TEIGENTFIGSDTMLVAPVVIGKNAKTAAGAVVTHDVADNEVVAGVPARPMKKKE"
     gene            9452..10714
                     /locus_tag="PLW25_02155"
     CDS             9452..10714
                     /locus_tag="PLW25_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOS53323.1"
                     /translation="MTLIAWLLLGVFVALDIFICIIRAALFNARLPQLIDLGTEDREE
                     LERTIKVLETTRLRATMRLLVAVLHALVLASLWLLLKTTYTQLSIGGLLGIFALAIFT
                     LLCFEFLAERFPLKDPETWAMRLAPLANFLDRLFSPFTSLLVGLQGSGDTVQRNLGAV
                     SEDELKTWVEVGEPEGGLEPDERKMIYSIFQFGDTLCREIMVPRIEVLALDVDTPLND
                     AINQFITTGHSRAPVYEEVIDNVIGLLYAKDLLKLLTSPAKNQTIRKFLRPAYFVPES
                     KKADELLAEMQARGIHLAVVVDEYGGMAGLVTLEDIVEEIVGEIRDEYDQSEERLVEK
                     ISDNEFVFLGRVSLDDFNEALDTELESDQTDTLGGYFYSQEGRVPVEGDELVFDGWRL
                     TVEGVHHRRVGRIRAVKMEKQDEDNDTN"
     gene            10692..11108
                     /gene="cdd"
                     /locus_tag="PLW25_02160"
     CDS             10692..11108
                     /gene="cdd"
                     /locus_tag="PLW25_02160"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOS53324.1"
                     /translation="MKTTTQIDGEMLEKLIETARNARNWAYAPYSHYAVGAALLTASG
                     KIYDGVNIENAAFPAGICAERVAMFIAVSEGERDFTFLVVMTDNGGSPCGSCRQVMAE
                     FGLSTRVLIVDTNGKTHIDTTVEGLLPWAFRPEDLL"
     gene            11115..>11491
                     /gene="recO"
                     /locus_tag="PLW25_02165"
     CDS             11115..>11491
                     /gene="recO"
                     /locus_tag="PLW25_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOS53325.1"
                     /translation="MPRSERNIRVEAVVLRHSDWGEADRLLVIYSREQGKLRAIAKGL
                     RKIQSRKAGHLEPFTRAAIMLAKGHDLWIVTQAEAIDLFQPLRENLQKMGQAAYVVEL
                     LDRFTYEEGQNWQLYQLLTETLT"
     assembly_gap    11492..11591
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11799..14795
                     /gene="glyS"
                     /locus_tag="PLW25_02170"
     CDS             11799..14795
                     /gene="glyS"
                     /locus_tag="PLW25_02170"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOS53326.1"
                     /translation="MSKAFAFQSVIMSLQKFWADQGCLIWQPYYSQVGAGTMNPATFL
                     RVLGPEPWKVAYVEPSIRPDDGRYGENPNRFQQHYQFQVILKPDPGNPQEIYLESLKA
                     IGIDPREHDIRFVEDNWQQPALGAWGLGWEVWLDGQEITQFTYFQQAGGLMLDPVSVE
                     ITYGLERITMPLQRVHHFREMRWNDTLTDGDVNLQAEVEHSKYYFEVADVERMRQLYQ
                     LYQAEAAEALGKGLVLPAYDYLLKCSHTFNILDTRGAMGVTERQAMFGKMREMARAIS
                     EAYVAQRQELGFPWMRGEKKTSAVEVKPIETHVFPDAPTDLLLEIGTEELPVADLQSA
                     IEQLIERVPLMLDELRLTHGQVSVAGTPRRLVVIVEDLAARQEAKETLVKGPPASRGY
                     DANGLPTKAAEGFAQSKGVRVQDLRAREIDGGTYLVALVKEDTRPTHQVLGEALPGLL
                     ESIKFEKTMRWNHTNVPFSRPVRWLTALLGGSTIPFEFAGLRATNTTHGLRFNEPTEI
                     TLTSLADYQAFLSSQGIILDPLRRKKTIQQQVNERCEQVGGRPLLEEELLEEVSRLVE
                     APTALLGRFDPAHLELPPEVLISVMKKHQRYFPVCNDAGKLLPFFVVVRNGDGHGADV
                     VTDGNEQVIKARFADAQFFIKEDMKHKLEDMLVRLGSLTFQHKLGSMLDKSRRVTLLA
                     DPLSGALGLSAAEKLTALRAAELCKADLVTHMVVEMTSLQGIMGQYYALHSGETQAVA
                     DAIREHYLPRYTGDSLPGSKAGLVVGLADRLDSLVGLFGAGLAPTGTHDPFAQRRTAI
                     GLVQLLTAFDIEFDLRQWLELAAQNLPLTISVENMQTCADFITARLRNILIEQGARYD
                     VVDAVLAEQANNPASAQRGVKELGNWVEREDWNSILPAYARCVRITRDQKQIYPVNPA
                     AFADPTEEALHEALVKAEQTPRRAGSVRDSLNVFLPMIPAVNAFFDSVLVMAEGPGLR
                     TNRLGLLQRVSAIMRGVADLSKLEGF"
     gene            14844..16739
                     /gene="dnaG"
                     /locus_tag="PLW25_02175"
     CDS             14844..16739
                     /gene="dnaG"
                     /locus_tag="PLW25_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOS53327.1"
                     /translation="MTAIDEIKARLDIVDIVSETVKLRRSGRSYSGFCPFHPNSKTPA
                     FAVFPDNGTWHCFGACNEGGDIFKFVMKREGWDFAETLKYLAERAGVTLTPMTPERKE
                     KEDQYEVMRQLLEESVKYYQFQLTKTPAGRAAYDYLIQRGLTPASIEAFELGYAPQSY
                     EALLGHLSGKGHALEQMLEVGLLVEKDSGGYYDKFRNRIMFPIRDGAGKMAGFGARVL
                     NPEDVPKFLNSPQTPLFNKGRLLYGLNKAKRAIRDADQVVLVEGYLDVIALHQNGFTN
                     SVSPMGTALNEDQLRYLKKYTRRIVLALDADAAGEKATLRGVEIARQALDREAELTYD
                     PRGLLKHEARLQADVRVTTLPPGLDPDDVVLKDRQAWQQVLESAKPIVVHVMETLAAG
                     QNLEDAKVKSAIAAQVTPLIEDVPSAVERDTYRQQLARLLKVDERSLMGLQKVRGRTR
                     RASAEAAQGTQMARSTPGARTVDSRTLQMEKHILSLLLNDTDRYYELERRIKSAGVEP
                     LSVADFDDAQNHELIKLLMKAMRQDAQEALDYLRENTTGDFTERLDELLSETNIKNTQ
                     TETILSDLYRTLISLRLIRVNQVINQLRFLQEEESADRDDQALQSMILSHTIARGKLD
                     LALAKTQ"
     gene            17035..17199
                     /locus_tag="PLW25_02180"
                     /pseudo
     CDS             17035..17199
                     /locus_tag="PLW25_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012367.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     gene            17671..18480
                     /locus_tag="PLW25_02185"
                     /pseudo
     CDS             17671..18480
                     /locus_tag="PLW25_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     assembly_gap    18616..18715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18716..19074)
                     /locus_tag="PLW25_02190"
     CDS             complement(<18716..19074)
                     /locus_tag="PLW25_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53328.1"
                     /translation="MRSFFNVGLLVGLCFAILLSSCAPRLAAAPLIVDPPSTPIPTLT
                     STPPATATVEIPAPVEPTFTPTSAACQINRVKAIPVFDQNGLLKSFDTPDKFIVLTID
                     DGYNNEVLNQMLDILEE"
     gene            19320..20591
                     /gene="rho"
                     /locus_tag="PLW25_02195"
     CDS             19320..20591
                     /gene="rho"
                     /locus_tag="PLW25_02195"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOS53329.1"
                     /translation="MKIIEMEKASLSSLRAIAEELKVPNAKRLKKENLIINIRRAEAE
                     QEGLEVRGGVLEITPEGVGFLRTNYSIGAEDVYVSQAQLRRHELRMGDLVIGVVRPPR
                     DAERHYGLLKVESVNGLAPDEMRNRKTFESLTPIFPDKRFDLETDKSILSSRLINLIA
                     PIGRGQRGMIVSPPKAGKTTILKQLANSITANNPEIHLIIALIGERPEEVTDMDRSVD
                     AEVVASTFDEPVSAHVRTAEITLERAKRLVEIGRDVVILMDSLTRLARAYNLVVNPSG
                     RTLSGGMDPSALYPPKKFFGAARNIEEGGSLTIIATALVDTGSRLDDVVYEEFKGTGN
                     MELHLSRRLQERRVFPAIDIERSSTRREDLLLGPDILPRVWLMRRMYLQMIATPPQGA
                     GMDPAVATEAILQRMARTKDNMEFLENLTED"
     gene            20592..21704
                     /locus_tag="PLW25_02200"
     CDS             20592..21704
                     /locus_tag="PLW25_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOS53330.1"
                     /translation="MTTKKTAPKVAVEPEEQTTPVKPAQPAPSWTVILSWVGTLFLVA
                     ILLAVLFRFNPADREKKSHEPLNVASDAQVELPALTSAQADYSLVRAASLDTTIPEGV
                     RHFAIKHTVEEGDSIFAIAKKYNLKPESILWANYDLLHDDPTYLNPGWQLTIPPTDGI
                     YYKLKEGDTLEKLAEKYYANVDDIISWPSNHMDITTPSVAGLEYVMIPGGYREIQSWI
                     VPLAYTSGSGATREIAGPGGCGSTNYVLIGSTAFIWPAVQYDLSGFDFSSYHLAIDIA
                     AGVGDAVYASDSGTVVYAGWNDSGYGNLVVIDHGNGYETLYAHLSQVYVSCGQGVYRG
                     GVIGAAGNTGRSFGSHLHFEVRLNGGFVNPWSVLGY"
     gene            complement(21756..24251)
                     /gene="priA"
                     /locus_tag="PLW25_02205"
     CDS             complement(21756..24251)
                     /gene="priA"
                     /locus_tag="PLW25_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOS53331.1"
                     /translation="MSNYARVSVNIAPLTGLFDYQVPDEYAPHIQPGSLVTVPFGRQT
                     AQGVVISLLETPAVDQTRPIATVVDDTPVLTSWQMRLAEQMARDNLATLPQCLDLMVP
                     PGLSQHADTLVTRSKQARTTALSSVQQRLLDLLEKRGPLRGRQVDTALPRVNWRESLS
                     ALVKAQLVVTQSVLPSPSVRPKVVRTALFSAPRGWRDDPGILVGRASSQAAQRRVAAL
                     EFLERQAIPVNISWVYAESGASSADLNRLAEMGLIILGETEIWRDPLEKLQPVLIHPP
                     VLTNHQQKVWEEIHHQLVNPSTPVPNLLLGVTGSGKTELYLRAAAAVVEKGRGVFILV
                     PEISLTPQTVKRFFARFPGKVGLIHSKLSPGERYDTWRRIRAGLLPIVIGPRSALFSP
                     LSDPGLIVIDEAHDPSYRQEDTQPHYHTVQCALSLSQINGACLLLGSATPDVETLYRF
                     TENHWPIHHLPQRILAHTSPDGQQPPSTQPNFTDLPQVEVVDMRKELSAGNRSAISQS
                     LHTALEEVLQRNEQAILFLNRRGSASYVFCRDCGFVLRCPRCETQLTWHADENQLICH
                     TCNYRRALPSQCPSCGGMNIRQFGMGTESLEKYLQGQFPSARLLRWDADTARYRGAHD
                     LILDHFIQHRADVLIGTQMLAKGLDLPLVTLVGVVLADISLNLPDFRAAERTYQLLTQ
                     VAGRAGRSARGGKVIFQTFQPGHYAIQAAAAYDAEGFYRTELAYRERTGYPPFSRLLK
                     FEFRHTDAAKAQHSAQTAGEQLKHWIDTENYRNTSLIGPVPCFYQKRAGYYRWQVIIR
                     GPDPRVVLEQHTFPDWKDPGLLVDLNLDPLNLL"
     gene            complement(24260..25102)
                     /gene="amrB"
                     /locus_tag="PLW25_02210"
     CDS             complement(24260..25102)
                     /gene="amrB"
                     /locus_tag="PLW25_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOS53332.1"
                     /translation="MNPHPPIRRSPIAGAWYSDNPHILRNEIKAYIDAATPPALPGEV
                     TGLIAPHAGYFYSGATAGHAYRCVKGRAFDVCVVFSPLHDYLPSQLLTSSHLAYATPL
                     GEVPINQHLVEQCSANIAKATGEILQPIANDREHSLEIQLPFLQVALSAPFSLVPIMM
                     RSQDPALLTAAAAAIAPALKEQNALLVASTDLSHFYTQSEAIQLDTSMLQQISAFSPE
                     GVLRAERCGEGFACGAGAVALMLWTEKELGATRVTLLHHSTSADSSGDASRVVGYGAL
                     AVTR"
     gene            complement(25188..26705)
                     /gene="lysS"
                     /locus_tag="PLW25_02215"
     CDS             complement(25188..26705)
                     /gene="lysS"
                     /locus_tag="PLW25_02215"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOS53333.1"
                     /translation="MELNELERIRCEKIQQMRDSGFEPYPTRTEVTSTTADAIKAFEN
                     AEKAGKPETLTTVLGGRLRAVRLMGKLAFAHIEDGSGRIQLFFRVNELGEAEMQRFKD
                     HFDLGDFIQAGGDLVRTRTGEVSLLVRNFKMLAKAITPLPAAKDEVVDGQVVRHATLS
                     DPETRYRQRYADLAVNQEVRQIFRTRAAVVRALRQFLDSRGFLEVETPILQPIYGGAA
                     AKPFVTHHNQLKQDLYLRISFELYLKRLTVGGFDRVYEIGRDFRNEGVSYKHNPEFTQ
                     LEFYWAYADYLKVMEMTEEMIAYVCDSVFGTRKLTYDGVELDFNPPWQRVNLRQSLIE
                     KANIDISLYPTAEALEEMMVSLDMKVKPGEARGKLIEQLLEEFVEPTLVQPTFMIDYP
                     RDISPLAKNLPGDPSTVERFEGFVCGMELCNAFTELNDPLEQEKRFLEMGRDYKADDE
                     EQHPMDEDYLKAMSYGMPPQGGFGMGIDRLVMLLTDQKNIREVLLFPPLRERDDQ"
     gene            complement(26814..27284)
                     /gene="greA"
                     /locus_tag="PLW25_02220"
     CDS             complement(26814..27284)
                     /gene="greA"
                     /locus_tag="PLW25_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOS53334.1"
                     /translation="MPTPVLTTEGYQKLQDELEYLRTTRREQIAQRLHEAIEGGELLD
                     NAELEAAKNEQAFVEGSIKELEILLATARVVNEAEIDKETVQVGNRVTIQEIGATQKE
                     EYVIVGAAEADPTLGKISNESPMGKALLGKRVGDKVQIEAPAGAYTVKVLKVDL"
BASE COUNT         6307 a         7110 c         7617 g         6224 t
ORIGIN      
        1 aaacacttgc gcgaagaact gggaatggct atcatctgga tcacccatga ccttggggtg
       61 gtggcgagcc tggctcaccg ggtgatggtg atgtacggcg gatacataat cgaagaagcc
      121 aatgtgaatg atctttttgc tgacccgcga cacccgtata cggtgggtct gctgggcagc
      181 ctgccgaaaa tccacgaaga tgtgcacaaa aaactatttt caattgaggg gatgccccct
      241 gttttgtaca agaaaccaaa cgcatgcccg ttcgcaccac gctgtaactg ggtgattgaa
      301 cgctgctggc aagaaaaccc caccctgaaa acagtaatgg atggacactc tgtagcctgc
      361 tgggtcgaca tcaggaacgg gagtaagaaa cgatgaccac caacaacaac gtattgatta
      421 aggttgaaaa cctggtaatg cacttcccaa tctatcgtgg tgtcatccgc cgccaggtag
      481 gtgtggttca cgctgtagat ggcatttctt tcacaattca acgcggagaa acgcttggcc
      541 tggtcggcga atcaggctgc ggtaagtcca ccacggggcg cgcgatctta caactttata
      601 agccaaccgc tggcaatgtc tactataaag ataccaacct ggttaagtta cgccctgaac
      661 ccatgcgctt gatgcgccga gaaatgcaga tgatctttca agacccttac gccagcctta
      721 acccgcgcat gaccgtgcgc gacattatcg gtgaaccatt gatggcgttt ggagaaaata
      781 acagcaagca aattgatgaa agagttgctt ctctgctgga catggtacaa ctcaaccccg
      841 atttttccag ccgttatcct cacgagtttt caggtggtca gcgccagcgc attggggtgg
      901 cccgggcact ggccttaaac ccttctttca tcgtctgcga cgaacccatt tcggcgctgg
      961 atgtttccat ccaggcgcag gtggtgaacc tgttggaaga actccagcaa gaattgaacc
     1021 tgacctatct cttcatcgcg cacgatcttt caatggtgcg ccacatcagc aaccgtgtag
     1081 cggtgatgta cctgggtacg tttgtggagc tggcggatcg caccacttta tatcgcaaac
     1141 ccctccaccc ttatacccag gccttacttt ctgcgatacc ggtggccgac cctacctaca
     1201 acgagcaacg ccgccgtatc atactggaag gcgatgtacc ttcccctgtc aaccccccgt
     1261 caggatgccg cttccgcacc cgctgccccc ttgcggacac gatttgcgcg gagcagtcac
     1321 ctgaatggcg ggaagtcgag ccgggacatt tcgcggcgtg tcataaaatt taaccgaagg
     1381 gtaaacacga ggaggtgatg acaattctgg tgaagatgct gcccataccg tatttgttgt
     1441 ctgtaaccga attttcaaaa gttctcacaa ggaggagaaa aaatgcgtaa ggtatatgta
     1501 ctgcttgctt tgttgatggt cgtaagcttg attcttacag cctgcgccca gccaaccaag
     1561 ccccctgtga ccgagccccc tgcgggtgaa gaaccccctg tggcagaacc tggtgaggtc
     1621 gtcgaggttg tttttaaccc ttacctgggt tccaataagt tggatggaaa tggcattccg
     1681 cccacattct tcgatgatgt ccatgtccgc aaggcgatgg cttactgctt cgattatcag
     1741 acagtcatcg acgatgtcta catgggcgaa gccgttcaat ccttaaccct ctctctgcct
     1801 ggcatgccgg ggtattcaaa cgataccgag cattacacgt atgacctggc aaaatgcgag
     1861 gaagagttca aactggctga tgtggacaaa gacggcatcc cggctggtga agatcccgat
     1921 gatatctggg aaatgggttt tcgcttccag atgctttaca acaccggtaa caccacccgc
     1981 gagatctaca cagagatcgt gaactcaaac ctcagcaaag tgaacaaaaa gttcgtggtt
     2041 gagaccctcg gtttaccgtg gccttcctat ctggctgccc aacgcgccca caaggtgccc
     2101 atgatgactg gcggctggct tgaagatatc cacgacccgc acaactggta ccagccttac
     2161 accaccggca cttatggcgg tcggcagaat ttacctgaag acctgaggaa tcagttcaaa
     2221 gcgctgttgg ataagggtgt gggcctggtg aaattcgagg atcgcgccga ggtttacaaa
     2281 gaatttaaca agctgtatta tgatcaggca gttggtattc ccctggtact ctccacttcc
     2341 cacgctttcg agcagaagtg ggtgcagggt cgcgtgatga acccgatctt ctccagcatt
     2401 tactactacc cgatctcaaa agcggcaggc gccaagaacc ccgatgtctt cactgtgatg
     2461 accaccggtt cacccgatac cttcgacccg gcgctgtctt atgacaccgc cagcggagag
     2521 atcgtccaga acgtgtacga aaccctggtc ttctatgatg gcgtttccac cagcgaatat
     2581 gttccccagc tggcatctga atactcagta tcagatgatg gaactgtctg gaccttcaaa
     2641 atccgtgaag gcgtcaaatt ccacgagggc ggcgacctga ccgcttctga tgtggcatat
     2701 tccttccagc gtggacttct acagggagga tattcctcac cgcagtggat gttggcggag
     2761 cctttcctcg gtgtcggcaa cgacgatatc accatgatcg tcgatgaagg cgcttctgct
     2821 gatgatcgcg aagcactgat ggccaatgat ccggctgttc tgaaagccgc ctgcgagact
     2881 gtaatgtcta aaatcgtggc tgatgatgcc accggtacgg taacgatgac gcttgcacag
     2941 ccctggggtc ccttccttgg caccatcgcc aatggctggg gttccattat ggataaagaa
     3001 tgggtcatcg aaaaaggtgg ttgggatggc tcctgcgata cctggcagaa ctactatggt
     3061 atggtgtctg ctgacgatcc gtttaccacg atcgccaatg gtaccggccc gttcaaattg
     3121 gattactgga cacagggcga agaagtatcc ctgctccgca atgacaatta ctggggtacc
     3181 cccgctgcgt taaagcaggt gcttatcaaa gacgttcccg aattcggtac ccgcttcgcc
     3241 gcgcttaagg ctggtgacgc tgatgtcatc gctgtgccga gagaaaatgc aacccaggtg
     3301 gatccaatgg tgagcgttgc caagttctac gacgcagaga ccaacaaata ccttgatgat
     3361 cagaacatct gcaccatcga taccactatc ctgggcaaag gacgctttga ggtttgcgct
     3421 gaagccaatg accgcccgct gcgcttgtac tacggtctgc cggcgctgtc acaggatgta
     3481 ctgctgttca cgttcttagt cgagtaagcg agaaccgctc tgtaagttat ttgcgggagc
     3541 ctttaaaagg ctcccgcaaa aaccttgatt tttctttgcg caatgcattg aattgcattc
     3601 aatccattcc gcacagaaaa acctgatttg aggtgtttcg atgaccaact acataatccg
     3661 ccgtttatta ctggtcccgg tgctgctatt tggcattaca ctgatcatct ttggtatgct
     3721 tcagttcctg ggcccggtgg agcggtcagc cttatatgta aaagacttcc ccaagaatga
     3781 taaacagatt caaagtgtta tccggcatta tggtttagac aaacccttct accaacaata
     3841 ttggatctgg ttgaacggcg tgattgaccc ggttacagga gaacgtagtg gtggtatttt
     3901 gcacggtgat tttggctatt cacgttctca atctcaaccg gtggctaacc ttattaccaa
     3961 caggtttcct aacacgcttg acctggcgct gtggacggtc ttccctatgc tttcagtagg
     4021 gatttggttg ggggtgcagg cagccgttca ccagaatggc gtcatcgacc aggccgccag
     4081 gatattcagt attgtgggca cttctttccc ggtctttgtg tttgggctgc tgatcttgat
     4141 gtttttctac gcggaactgg gttggtttcc gccggggaag aattcaaact gggtgacgca
     4201 ggaaattatg caggggaatt ttcaccaata caccaaattg ctcaccatcg acgcgctgct
     4261 gaatggacgg ctggatattc tttgggacgc gctgcgccat atggtgctgc caatcctgac
     4321 cctttcgtac atcacctggg caacttttgt gcgcgttacc cgttcttcga tgcttgaaac
     4381 cctgcgtatg gattacgtgg tcaccgcacg cgctaaaggg cttaaagaaa aagatgtgat
     4441 caataaacac gctcgcccaa acgccatgct taccattgct acgatggctg gtatgagcgt
     4501 ggtgggtttg ctgggcggcg tggtgattac agaaaccgta tttaattacc ccggcatcgg
     4561 gtccgcggca gccgaagcgg ctcaacaact ggatgtggtc accacacttg ggttcgcgat
     4621 ctttaacggt ctgatcttaa ttgtggctaa cctgatcgtc gatgtattgt acggcgtcat
     4681 agacccgcgc gtgcgcctgg actgaggaga acaacatgac tgaaacacca agaccgacag
     4741 gtgacaacac cctgctaaaa gaagtggtac cagaccgcaa aatcagccgg gcggaaagac
     4801 tccttgggcc tgaaaactat cgcatcctta agggtttgtt aaaaaccccc gcgtcgatta
     4861 tcggcttcat cctgattgga atatttattt tcgtcgcggt ttttgcgccg ctgatcgctc
     4921 caccggtgaa tgatgaccct ttcaaaatcc cgcgagatgg cttcagcggt caaccccgcc
     4981 caccgggcac tgagtggaag caaaatgtac cagaggtgcc tttttggtac aaaccactga
     5041 ccggtaagga tgaatgggtg cacctgatgg gaaccgccca agggcagtat gatatattct
     5101 acagcgtcat ctggggttcc cggactgctt tccgcactgg attgatcgtt gtattggcta
     5161 cagtgatgat tggtatcatc cttggttcag tctctgcgta ttacggcaaa tgggttgacc
     5221 agctgatcat gcgcattgtg gatgttttta tgactgtacc ctatattatg atggccttga
     5281 tcatggcggc ggtactgacc ccggtcattg gcaggagcct ggttcctgcc ttactggcga
     5341 tgatcacctt tggctggatg ggttacgcgc gtattattcg cagcgatatc ctctcggtca
     5401 aacagcgcga ttttattctg gcggcgcgtg tgtgcggggt gaaagacggt cgtatcttgt
     5461 ttaagcatat cctgccaaac gccatcttcc ccaccctggt actggcttcg cttggtattg
     5521 gcgatgtggt gctttccttc gcggcgctgt cctttctcgg tatcggcact cccatgggtt
     5581 acgcggattg ggggcaggtg ctcagttttg cgcgcaactg gatcaccaac ctggctgact
     5641 actggtatat cgtcgtttac cctggtttga cgctggtgtt tttcgtgatg ggctggaacc
     5701 tggtggggga tgccctgcgc gatgtgttag accccaaaat gcgcggacgg tcataaagcg
     5761 cgcactcgaa cctcaacggc ggcagctcac agccgccgtt ttttgttccc ggtctatgct
     5821 ttataattga cacgaggata atcgatatga gaatgagaac aaggcttaca tctattatag
     5881 ccctgctgtc ggtgatgctg gcagcgctgt ttttactgag cgcctgccac aaagaacaaa
     5941 tagctggcaa cccgcctgtc aatacgccca ccgtggaacc gcccactccc acttcgccgc
     6001 cccccaagac gctggtcgtg tgcctggcca atgaacccgg tgacctgtac atctatggcg
     6061 atacttcgcg taccaagtgg agcgtgctgg aggcgcttta tgatggaccg gtcgacacgc
     6121 tcaattatga accctccgcg gtaatccttg cttctctccc ttcgcaggaa aatggcgcgg
     6181 tgagcctgcg ctctgtggcg gtcagtgagg gtgacccggt cgcaaatgtg aacggagaca
     6241 tcgtcgcgct taagaagggc acgcgcgttt tcccggcggg ctgcacctcc cctggctgcg
     6301 cggtagagtg ggacggcacg accgcgctcg aattgactca gatggtggtg aacttcaccc
     6361 tgctggacgg cgtcacctgg tcagatggcg aagcgcttaa agcgcaagat tcggttttct
     6421 ccttcagcgt cagcgccgac gaggcaacca gggtccttaa aaccaacatc gagcgtactt
     6481 tctcatatac tgcgctggat gatcacacgg tggaatggat cggtcagcca ggctatctta
     6541 cgcttaaccc cgcggctttt ttctggtcgc cgctgccaga acacgcgctc gccgggctca
     6601 ctcctaccca gatgaacgaa gaccccctga ccacgcgcca gccccttggt tggggcgcgt
     6661 ataagatgga cgaatgggtg agcggcgacc acatccgcct ggtgaagaac tctcattact
     6721 tcagagcggc tgaaggtttg ccctatttcg atgtgcttgt gtaccgcttc attccctcca
     6781 cgcctgaggt tgacctgagc atgatgttga ccggtgaatg cgacatcata gatacctccg
     6841 ccgggttgga gtcacagatc ctgcgcgtgc gtgaactgga aggacaggga aagctcaaag
     6901 cttactttgg catgggtcca gaatgggagg ggctgtactt cggtattaaa ccggcaactt
     6961 acgatgatgt ttacaacccg tatgaagacc gtcaggattt cttcggagat gtgagagtgc
     7021 gccaggcgat cgcctactgt gtggaccgcg agtacatcca aacgtatatc accctgaacc
     7081 aatcggtcat ccccagcact ttcctgccgc ccaatcatcc tttcgcggcg gcagagacgc
     7141 ggagctacac gcacaacccc cagttgggca tccagctgct cgaagaagca ggctggcagg
     7201 atagcgacgg caaccactca acccccaggg tggcggcaga tattaagaat atcttctaca
     7261 acaccgaact atctctgaat tactacgtta ccgagagtga actgcacgcg gctgtgaccg
     7321 aagcggtggt gaaatcgctg ggtgagtgcg gcatcaaggt cacgcccaca ttcctttctg
     7381 tgggcgaaat gttcgcgcct ggccccgaag ggcggatctt tggccgcaat tttgacctgg
     7441 cggagctggc gtggtcaact ggcaggcagc cgccctgttt cctgttctct tcttcagaga
     7501 tcccttcagc aaagaacaag tggttgggca cgcgctatgg aggggtcaac ctcagcggat
     7561 acgccagcag cgaatacgac gctgcctgtg aacaggcgct ttcagccggg ttggaccgcg
     7621 atttgctcac cgcccaatcc gctaacgcgc aacagatcct gatggaagac ctgcccgcgc
     7681 tgcccctgtt ctttcacgtc aaagccatgg tttcacgacc tgacctgtgc gggttgcaac
     7741 tggatgtgag cgcccgcagc cctttgaaga acattgaagc tctcacgctc ggagagacct
     7801 gcccgggtaa atgagagggc aggcgggaga cgccattcca acaggttttg tgttagaatc
     7861 taacccactg cggatggagt gtattggggc gtggtgcaat ggcagcacac cagactttgg
     7921 atctgtggat cttggttcga atccaggcgc cccagcctgt gcagtaagtg aatgaaaaag
     7981 aaatcagaac ctcccagatc tcaatccgtg tttttcggct cagctgccac aagttggatg
     8041 gcgcgcggct gtgtttaatc cggtgtagaa taaaatgact cgctggtatc tttcgtggag
     8101 agaaatatga atctttctac catcatcctc gctgccgggc agggaacgcg tatgaactca
     8161 accctgccta aagtgctgca tcctttaaat gggaaactgc ttgtaaaata ttccatcgat
     8221 ctggcggacg cgctctcttc cagtagaaca gtggtggtga ttggaaacgg cgctgagcag
     8281 gtccgcagtg ctctgggaga atctgtagcc tacgcgctgc agccgcagcg cctgggtacc
     8341 gccaacgcgg tgcaggcggc tgaagggttg ttggctgacg ccagtgggtt ggtgcttgtc
     8401 atttcagcgg acatgccgct gctcaccgcg gataccctac agaggctggt caccacacaa
     8461 tcacaaaaca ccggcccaat ggccctgctc accgtgcgct cagccaatcc acgcggcttt
     8521 gggcgcatcc tccgcggcgt ggatggagga gtgacaggca ttgttgaaga agcccaggcc
     8581 acggctgagc aattgaaaat tgatgaatta aatgtcggcg cttattgttt caattcttcc
     8641 tggttgtggc aggcgctgcg gaagatccag ctttcaccga agggtgaata ttacctcacg
     8701 gacatcgtag ccgtggctgt ggcagaaggt tcgcctgttt ccagccttac agtgacagac
     8761 gaggaagaaa cccttggcat caacaaccgc gtgcacctgg cggaagccga gcgcgtcatc
     8821 cgacgacgca tcaatcaagc gcatatgctc gccggcgtaa gcatgactga ccctgaactt
     8881 gtttatatag aagaaagcgt gaaaattggt cgagacacca ttctgctgcc caacacctgg
     8941 cttagcggca gcaccatcat cggcgaaggc tgcgaaattg gaccgaacac cttcatcaat
     9001 gactccacaa ttggcaatca ctgccacctg ctggcgtcgg tggttgaagg cgcggtagta
     9061 gaagacgagg ttgggatggg tccgttctgc cacctgcgca aaggggcgca cctggggcgt
     9121 ggtgtgcaca tgggtaattt cggtgaggtg aaagactctt acctggcggc aggggtcaag
     9181 atgggtcatt tttcgtacat tggcaacgcc acgatcggcg aaaacgtcaa tatcggcgcg
     9241 ggcaccatca cctgtaattt tgacggcgtg aacaagaacc ccaccgaaat tggtgaaaac
     9301 accttcattg gctccgatac catgctggta gcccccgtgg tgattgggaa aaacgccaaa
     9361 accgccgccg gcgcggtggt gacgcatgat gtcgccgaca atgaagttgt tgcgggtgtt
     9421 cctgcccgcc caatgaagaa aaaggaatga agtgaccttg atcgcctggt tgttacttgg
     9481 cgtgtttgtc gcgctggata tttttatctg catcatacgg gcggcgcttt ttaatgcgcg
     9541 cctgccgcag ctgatcgacc tgggcacaga agaccgggaa gaactggagc gcaccatcaa
     9601 ggtgctggaa accacccggc tgcgcgcaac catgcgctta ttggtagccg tgctgcacgc
     9661 cctggtactc gcctccctct ggttattact gaaaaccaca tatacccagc tttcgatcgg
     9721 cggcttgttg ggcatcttcg ccttagccat attcacgctg ctctgcttcg aattcctggc
     9781 agagcgtttt ccactgaaag accccgaaac ctgggcgatg cgcctggcgc cgctggcgaa
     9841 ttttcttgac cgacttttca gcccgttcac ctcgctgctg gtgggtctgc agggctccgg
     9901 tgatacggta cagcgcaacc tgggggctgt ttcagaagat gagctgaaga cctgggtgga
     9961 ggttggcgaa ccagaaggcg ggctggaacc tgatgaacgc aagatgattt actccatctt
    10021 ccagtttggt gatacgcttt gccgtgaaat aatggtgccg cgcattgaag tactggcgtt
    10081 ggatgtggac acgccattga acgacgccat taatcaattc atcaccacag ggcattctcg
    10141 cgcgccagtg tacgaagagg tgattgataa tgtgatcggg ttgctctacg ccaaagacct
    10201 gttgaaactg cttacttcac cagccaagaa tcagaccatc cgcaaattcc tgcgcccggc
    10261 ctattttgtg cctgaatcaa aaaaagcgga tgagctgctg gcggaaatgc aggccagggg
    10321 gattcacctg gcggtggtgg tggatgaata cggcggcatg gcgggattgg taacccttga
    10381 agatatcgtc gaagaaattg tgggcgagat ccgtgacgag tacgatcaaa gcgaagaacg
    10441 gctggtagaa aagatatcgg ataacgagtt tgtcttcctg ggcagggttt cgttagatga
    10501 ctttaatgaa gcgctggata ctgaactgga gtctgaccaa acggatacgc tgggagggta
    10561 cttctacagc caggaaggca gggtacccgt tgaaggggac gagctggtct ttgatggctg
    10621 gcgtttaacg gtggaaggag tgcatcaccg ccgggttggc cggatacgcg cggtgaagat
    10681 ggagaaacag gatgaagaca acgacacaaa ttgatgggga gatgctcgaa aagttaatcg
    10741 aaaccgcccg aaacgcgcgc aattgggctt acgcgcctta ttctcactat gcggttggcg
    10801 cagcgctgct caccgccagc ggtaaaatct acgatggcgt caacatagaa aatgctgctt
    10861 tcccggcagg tatctgcgcc gagcgagtgg cgatgttcat agccgtttca gagggagagc
    10921 gtgatttcac ttttctcgtg gtgatgaccg ataacggcgg ttcaccctgt ggttcctgcc
    10981 gccaggtgat ggctgagttt ggtctttcta cccgtgtgct gattgtggat accaacggca
    11041 aaacgcatat agataccact gttgaggggt tattaccctg ggcgttcagg cctgaagacc
    11101 tgctttgagg ttctatgccc aggagcgagc gcaatatccg ggtggaggcg gtggtgctgc
    11161 gccattcaga ttggggcgaa gctgaccggc tgctggtaat ctattcgcgt gaacagggca
    11221 aactacgcgc catcgccaaa gggctgcgca aaatccaatc gcgtaaagcc ggtcacctgg
    11281 aaccatttac ccgcgctgcc atcatgctgg caaagggaca cgacctgtgg attgtgaccc
    11341 aggcagaagc catcgacctt tttcaaccct tgcgtgaaaa cctgcaaaag atggggcaag
    11401 cggcttatgt ggtggaactt cttgatcgtt tcacatatga agagggacaa aactggcaac
    11461 tgtaccagct cctgaccgaa acacttacca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn ntgcgcgccg ctccgccgcc tgccattcgc caggaaatcg atgccctgca
    11641 gcattactat ctcacctatt tactcgaacg cagcttgaac tcgccagaat tccttaaaca
    11701 aattcgcaag caaatgtgac cccgcccgcc accagcggcg ccccttgctc tcggggtata
    11761 attggaacac ttcaaagtca caccttcccg gagaattaat gagcaaagca tttgcgtttc
    11821 aatccgtgat catgtcgttg cagaaattct gggcagacca ggggtgcctg atttggcagc
    11881 catattacag ccaggtgggc gccggtacga tgaacccggc gaccttcttg cgcgtgctgg
    11941 gcccggagcc ctggaaagtg gcgtatgtgg aaccatccat caggccggat gacggacgtt
    12001 atggggagaa cccgaaccgc tttcagcagc attaccagtt ccaggtgatt ctcaaacctg
    12061 atccgggtaa cccgcaagaa atctacctgg aatcattaaa ggctatcggg attgacccga
    12121 gagaacacga tatccgcttt gtggaagaca actggcagca accggcgctg ggcgcctggg
    12181 ggctgggctg ggaagtttgg ctggatggtc aggaaattac ccagttcacc tattttcagc
    12241 aagcgggtgg gttgatgctc gacccggtct ccgtggagat cacctacggg ctcgagcgca
    12301 ttaccatgcc cttacagcgt gtgcaccatt tccgcgagat gcgctggaac gacaccctca
    12361 cggatggtga tgtgaacctg caggctgaag tggagcatag taaatattac tttgaagtgg
    12421 ccgatgtgga gcgcatgcgc cagctctacc agctgtatca ggcagaagcc gcagaagcgc
    12481 tggggaaggg attggtgttg cctgcttacg attacctgct gaaatgctca cacaccttca
    12541 atatattgga tacccgcggc gcgatgggtg tgaccgagcg ccaggcgatg tttggcaaga
    12601 tgcgcgagat ggcacgtgcg atctcggagg cttatgttgc ccagcgtcag gaattaggct
    12661 ttccctggat gagaggtgaa aaaaagacat cggctgtaga ggtgaagcca atagaaaccc
    12721 atgttttccc cgatgcaccc accgatctat tattggaaat tggcaccgaa gaactgccgg
    12781 tagctgacct gcagagcgcg attgagcagt tgattgaacg cgtaccgttg atgctggatg
    12841 aattgaggtt gacccatggg caggtgtctg tggcggggac gcctcgccgg ctggtggtaa
    12901 tagtggagga cctggcggcg cgccaggaag ccaaagaaac cctggtcaaa ggtccaccag
    12961 ccagccgtgg ttatgatgcc aatggattac ccacaaaggc agctgaaggc tttgcccaaa
    13021 gcaagggcgt gagggtgcaa gacctgcgcg cgcgcgagat tgacgggggt acctacctgg
    13081 tagccctggt aaaggaagat acccgcccaa ctcaccaggt actgggcgaa gcgcttccag
    13141 ggttgctgga aagtattaag ttcgaaaaga ccatgcgctg gaatcatacc aatgtgccat
    13201 tttcgcgccc ggtgcgctgg ttgaccgcgc tcttaggcgg ttctaccatt ccttttgaat
    13261 tcgctggttt gcgggccacg aataccacgc atggcttgcg gtttaatgaa cctactgaaa
    13321 taactctcac ctctctggcg gattatcaag cctttctttc gagccaggga atcattctcg
    13381 acccactacg gcggaaaaag acgatccagc agcaggtgaa tgagcgctgt gaacaggtgg
    13441 gcggcaggcc attactggaa gaagaactgt tggaggaagt gagccggctg gtagaagccc
    13501 ccacagcgct attgggccgg tttgaccccg cgcatctcga gttgccaccg gaagtcctca
    13561 tttcagtgat gaagaaacat cagcgatact tcccggtgtg caatgacgcg ggcaaattac
    13621 tgccgttctt tgttgttgtg cgtaatgggg atggtcacgg cgcggacgtg gtcacagatg
    13681 ggaatgagca ggtcatcaag gcgcgttttg cggacgcgca gttcttcatt aaggaagata
    13741 tgaaacacaa acttgaagac atgctggttc gcctgggctc gctcacgttt cagcataagc
    13801 tgggctcaat gctggacaaa tctcgccggg ttacccttct ggcagacccc ctgtcaggcg
    13861 cgttgggtct ctccgcggcg gaaaagttga ccgctttgcg cgccgctgaa ttatgcaagg
    13921 ccgacctggt cacgcacatg gtggtggaaa tgacctcgct gcagggcatc atggggcagt
    13981 attatgcgct gcattctgga gaaacgcagg ctgtcgctga tgccatccga gagcactacc
    14041 ttccacgcta caccggagac agtcttcccg gctcaaaggc aggactggtc gttggattgg
    14101 ctgaccggtt ggattcgctg gtgggtctct ttggcgctgg tttggcacct accggcaccc
    14161 atgatccctt cgcgcagcgc cgcaccgcca ttgggcttgt tcagttactg acggcgtttg
    14221 atattgaatt tgacctgcgc caatggttgg aactggctgc ccaaaacctg ccgctcacga
    14281 ttagcgtgga aaacatgcag acctgcgctg atttcatcac cgctcgcctg cgcaatatct
    14341 taattgagca gggcgcgcgt tatgatgtgg tggatgcggt tctggcggaa caagccaata
    14401 accccgccag cgcgcagcgc ggggtgaagg aactggggaa ttgggttgaa cgggaggatt
    14461 ggaacagcat cctgccggca tatgcccgct gtgttcgcat aacccgggat cagaagcaga
    14521 tctatccggt taatccggcg gcgttcgcag accccacaga ggaagctctg cacgaagcgt
    14581 tggtgaaggc tgaacaaaca ccacgccgcg cgggttctgt cagggattcg ctgaatgttt
    14641 tcctacctat gatcccggcc gtcaacgcct tttttgattc tgtactggta atggctgaag
    14701 gccctggttt gcgcaccaac cgcttagggc tgttgcagcg tgtttccgca ataatgcgcg
    14761 gagttgctga cctgtcgaag ttggagggct tttagtcatt cgctcaacag agccgaaccg
    14821 ttttctgtac tacaattgat cctatgaccg cgattgatga gataaaagcc aggctggaca
    14881 tcgtggacat cgtatccgaa acggtgaaat tgcgccgttc cggcaggagt tattctgggt
    14941 tttgcccttt ccacccaaat tctaaaaccc cggctttcgc ggttttccct gataatggca
    15001 cctggcattg ctttggcgcc tgcaatgaag gcggcgatat attcaaattc gtgatgaagc
    15061 gtgaaggctg ggattttgcc gaaaccctca aatatttggc ggaaagggcg ggcgtgaccc
    15121 tgacgcccat gacgcctgaa cgcaaggaaa aagaagacca gtacgaggtt atgcgccagc
    15181 tcctcgaaga aagcgttaaa tactaccaat ttcaattaac gaagacacct gcggggcgag
    15241 cggcgtatga ttacttgata caacgcggtc ttacacccgc gtccatagaa gcctttgaac
    15301 ttgggtatgc cccgcaatct tatgaagcgc tgctgggtca tctgagcggc aaggggcacg
    15361 cgcttgagca aatgctggaa gtggggctgc tggtggagaa ggactctggc ggttattatg
    15421 ataaattccg caatcgtatc atgttcccca ttcgcgatgg cgccggaaag atggccggct
    15481 ttggcgcgcg ggtgcttaac cccgaggatg ttcccaaatt tctaaactca ccgcagactc
    15541 ctttgttcaa caaagggcgg ctgctctacg gtttgaacaa ggcgaaaaga gctattcgtg
    15601 atgccgacca ggtggtgctg gttgagggct acctggatgt gatcgccctg caccaaaacg
    15661 gttttaccaa cagtgtttct ccgatgggca cagcgctcaa cgaggatcaa ttgcgctacc
    15721 tgaagaaata tacccggcgc atcgttcttg cgctggatgc cgatgcggcg ggcgagaaag
    15781 ccacactgcg cggtgttgaa attgcccgcc aggcgcttga ccgtgaagcc gaattgactt
    15841 atgaccctcg cggtttgttg aagcatgaag cccggctgca ggctgatgtg cgtgtgacca
    15901 ccctgccccc ggggttggat ccagatgatg tggtgcttaa ggacaggcag gcctggcagc
    15961 aggtgctgga aagcgccaag cctatcgtgg ttcacgtgat ggaaacgctg gcggcgggtc
    16021 aaaaccttga agatgccaaa gtgaaatctg ccatcgcggc gcaggtgacc cctttgatcg
    16081 aagatgtccc ttccgccgtc gaaagggata cttaccggca acagctggcg cgccttttga
    16141 aagttgatga acgttcatta atgggcctgc aaaaggttcg tgggcgaact cgccgcgcca
    16201 gcgcagaagc ggcgcaaggc actcagatgg caagatcaac gcctggcgcg agaactgtgg
    16261 atagccgaac cctgcaaatg gagaaacaca ttttatcgct cctgctgaac gatactgacc
    16321 gttattacga gttggagagg cgtataaagt cggcgggggt tgaacctctt tcggttgcag
    16381 attttgatga cgcgcaaaat catgaattga tcaagctgct gatgaaagcc atgcggcagg
    16441 atgctcaaga ggccctggat tacctgagag agaacaccac gggtgatttt actgaacgac
    16501 tggatgaact gctctcagaa acgaacatta aaaacacgca aaccgaaacg atcctgtctg
    16561 acctgtaccg cacgctcatc tcgttacgcc tgatccgcgt aaaccaggtc atcaaccagc
    16621 tgcgcttcct ccaggaagag gagagcgcag accgcgacga ccaggcatta cagtccatga
    16681 ttttgagtca cacgattgct cggggaaaat tggaccttgc cctggctaaa acgcaataaa
    16741 aaagagacaa aacacttatg gtatagttaa cccatgtcct ccggtcaaaa aaaatccgca
    16801 caaagccctt cgtttcagca ggatgctcct gctgaagtaa acgcggtggt gaagacggct
    16861 gtcaaacctc acatgaaagc aaaaaaacca caacgcccat cagcgcgcgc gaaaattgaa
    16921 cacaaaacca tcggcgcagc gggcaaacca ggttctcaac cgggcagcca aaaagttgtg
    16981 aatgctacgg tgaaaacccc tgaaattcaa gatacagaag aacccggaac cgcgatggga
    17041 gcggctgaat ggaacgagat tgagattgaa gacccggctg agatccttga agacccggct
    17101 atcgcgctgg agctgaccga agatcctgtc cgcctgtatc tgaaagaaat cggtcaaatc
    17161 aacctgctgg acgcggatgc tgaattcagg ctggcagcca ggatcgaagc ctgtgtgaga
    17221 gtaaattctg tgcgtcaaca cgtccgaccc tccccgggaa aagaaaaaca ctaccgcgcc
    17281 ctgtttaccg ccctgctttc agaattgacg acttcgtgga ggcgtttgca ggaggacaca
    17341 gaacgtatcg ggttgatcga atgtcctgat ttcgcgctga ccctcgctga ggcgcagctc
    17401 ctcaggcact cgtggcaatc ggacgaaccc tcctacctgc gcaaccacct ggaccacgcg
    17461 ctttggggca aagaccccgt ttgggaagga ctggtcaggc aggcttttac ggtttttata
    17521 tgcatgtatc tgttgccaga acgagtatcg aacgctctat ttgaatatgt catccagcac
    17581 gacaccattc ctgatatggc ctggctgaga agacgattac cggatgaaaa agcgctggaa
    17641 accgaggttg aaggggtctt caaccgggct gaagaagcca atcagacttt gattcgagcc
    17701 aatttacggc tggtggtgag catcgccaag cgctaccttg ggcggggtat ttcgtttctc
    17761 gatttgattc aagaaggcaa cctgggttta ctgcgcgcgg tgacgaaatt tgattctacc
    17821 cgtggattta aattcagcac ctacgcgacc tggtggatca ggcagtccat cagccgttat
    17881 atcgctgagc acgcccgcac gatccgtata ccggtgcacc tcttcgaagc cattacgcgt
    17941 attttacggg tgcagcgcag cctggtgcag gagcttggca gggaaccaac gcttgaagag
    18001 ctggctcttg aatctgacat tatctcaccg gaagacgcag cggcaatcaa aatggcacgg
    18061 tttaccaatc agccgcttga gggagaaatg cagcggcgct ggtcctgggc gactttgaag
    18121 gtgcagcgca ttcttcaatc cgccgaggaa ccgatctctt tggaacggcc agtgggtgat
    18181 gaagaaagca gccaactggg ggatttcatt gaagatgatg aggcgttgga acccatggat
    18241 gcggcagccc gcgagatgtt gcgcgaacag gtgcaaaacg cgctggctgc cttatcagag
    18301 cgtgaacgtc aggtgctgga actgcggttt gggttgatcg atgggaaaga ccacacgctg
    18361 gaagaagtga gccgttattt caacgtgaca agagaacgca tacgccagat agaagccaaa
    18421 gccttgcgta aactgcgcca tcccacacgc agccgttttc taaaagaata cctgggataa
    18481 caaacaccct ggcagcagtt aattgcaagt ttcgctcagt gcttcgcgca ggctgatgat
    18541 ctgccaatcc gttttcataa gttgctgcaa ctcatccagg ttttccggga tgatgtgaag
    18601 gatcagaatg ccaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgctc
    18721 ttccaggatg tccagcatct ggttgagcac ttcgttattg tagccatcgt cgatggttaa
    18781 gacgataaat ttatcagggg tatcaaacga tttcaacagg ccgttttgat caaataccgg
    18841 gatggctttc acgcggttta tctggcaggc cgcgctggtt ggtgtgaagg ttggttcaac
    18901 cggcgcgggt atctccacag tggccgtggc tggcggcgtg gaggtgagcg tgggtatagg
    18961 tgttgaaggc gggtctacaa tcagtggggc agcggccaac cgtggggcgc aagacgaaag
    19021 cagaatggca aaacacaaac ctaccaataa accaacgtta aagaatgaac gcattgacac
    19081 tcgctttgaa tgaatttgac aacagggtaa ttttactcaa tacccttgac ttgcgtcagt
    19141 tttttttcca tgatatactc attttacttg agtaatcaca ataatgaaaa ggttatttcc
    19201 ggcttcacga gtgaatccgg aagacaaaaa atcccgtcac taaaaaacct atcagctttc
    19261 ccgagcctga ccccaggtcg gggtgccaat ctccaaccta tactgatgga catgaaccta
    19321 tgaaaataat tgaaatggaa aaagcgtccc tatcctcgct gcgcgcgatt gcggaagagc
    19381 tcaaagtccc caacgcaaaa agactgaaaa aagaaaatct gatcatcaat attcgcaggg
    19441 cggaagccga gcaagaaggg cttgaagtgc gcggcggcgt gcttgagatc accccggaag
    19501 gcgtgggttt cttacgcacc aattattcaa tcggtgcgga agatgtttac gtctcacagg
    19561 cgcagctgcg gcggcatgaa ctgcgcatgg gagacctggt cattggcgtg gtgagaccgc
    19621 cgcgagacgc cgagcggcat tacggcttgc ttaaggtgga gagtgttaac ggtctcgccc
    19681 cggatgaaat gcgcaaccgg aagacctttg agagccttac tcccatcttt ccagacaagc
    19741 gctttgacct ggaaaccgat aagagcattc tttcttcgcg actcatcaac ttgatcgctc
    19801 ctattggacg tggtcagcgt ggaatgattg tatcgccccc aaaggcggga aaaacgacca
    19861 tccttaaaca gctggccaac tctatcaccg ctaacaaccc cgagatacac ctgatcatcg
    19921 cccttatcgg tgaacgtcct gaagaggtaa ctgatatgga ccgctcggtg gacgcagaag
    19981 tagtggcttc aacttttgat gaaccggtca gcgctcatgt gcgcacagct gaaattacgc
    20041 tggaacgcgc caaacgcctg gtggaaattg gccgcgacgt ggtcatcttg atggattctc
    20101 tcacccgcct ggcgcgcgct tataacctgg tcgtcaatcc gtcgggtcgt acactctcag
    20161 gtggaatgga cccctcggcg ctttacccac caaaaaaatt cttcggtgct gcgcgcaata
    20221 tcgaagaagg aggttccctc accatcatcg ctaccgcgct ggtagatacc ggctcgcgat
    20281 tggatgatgt ggtttacgaa gagttcaaag gcaccggcaa catggagctg catttatcgc
    20341 gcaggctgca ggaacggcgc gtcttcccgg ccattgatat tgaacgctca tccacccgcc
    20401 gtgaagacct gctgttggga ccagatattc tgccgcgcgt gtggttgatg cggcgtatgt
    20461 acctgcagat gatcgccacc ccgccgcagg gcgcgggtat ggacccggca gtggcaaccg
    20521 aagcgatctt acagcgtatg gcgcgcacca aagacaatat ggaattcctg gagaatttaa
    20581 cggaggatta attgacaacg aaaaaaacag cccccaaggt ggctgtagag ccggaagaac
    20641 agacgacgcc tgtaaaacct gctcaaccag cgccttcctg gactgtcatt ctctcatggg
    20701 tgggaactct tttcctggtg gcaatcttgc tggcagtgct gtttaggttt aaccctgctg
    20761 atagagagaa aaagagccat gaacctttga acgtagcctc tgacgcgcag gtggaactac
    20821 cggctttaac ttcggcccag gcagactatt ctttggtgag agccgccagc ctggacacga
    20881 ccatacctga aggggtgcgc catttcgcca tcaagcacac cgttgaagag ggtgattcaa
    20941 ttttcgccat tgccaaaaaa tacaacctta aaccggaatc aattttgtgg gcgaattatg
    21001 acctgctgca tgacgaccct acgtatctaa atcccggctg gcagttgacc atcccaccca
    21061 cggatggcat ctactataag ttgaaggagg gagacaccct agagaaattg gcagagaagt
    21121 actacgcgaa tgttgatgac attatcagct ggccatctaa ccatatggat attaccaccc
    21181 catctgtcgc ggggttggaa tatgtgatga ttcccggcgg atatcgtgaa atccaatcct
    21241 ggatcgtgcc cctggcatac acctctggtt caggcgcaac gcgcgagatc gccgggccgg
    21301 gcgggtgcgg ttcaaccaat tatgtgctta tcggttcaac tgcgttcata tggccggctg
    21361 tgcaatatga cctttcaggg tttgatttct ccagttacca cctcgcgatt gatattgccg
    21421 ctggtgtagg agatgcggtc tacgcgtctg atagtggcac agtggtgtac gctggttgga
    21481 atgactcagg ttatgggaac ctggtggtga tcgatcacgg taacggctac gaaacgcttt
    21541 acgcccacct ttcgcaggta tatgtctctt gcggtcaggg agtttacaga gggggtgtta
    21601 tcggcgccgc gggaaatacc ggtcgctcct tcggttcaca ccttcatttt gaagtgcgct
    21661 tgaatggcgg ttttgtcaat ccctggtcgg tgttgggtta ttagattata aaaaaaaaca
    21721 agtgaggcgg gagggatttc cctcccgctg ggcttttaaa gaaggttcaa tgggtcgaga
    21781 ttcaggtcta ccagcaaccc ggggtccttc caatcaggaa aagtgtgttg ctcaagcacc
    21841 acgcgcgggt cagggccgcg gatgatcacc tgccagcggt aataaccagc gcgtttctga
    21901 taaaagcagg gcactggccc gatgagcgag gtgtttcggt agttctctgt gtcaatccag
    21961 tgtttgagct gttcgccggc ggtctgcgcg ctgtgttggg ctttggctgc gtctgtgtgc
    22021 ctgaattcaa attttaaaag gcgggaaaat ggcggatacc cggtgcgctc tcggtaggcc
    22081 aactcggttc tgtagaaccc ctccgcgtca taagccgcgg cagcctggat ggcgtaatgc
    22141 ccgggttgga aggtttgaaa aatgactttg ccgccgcgag cgctgcgccc cgccctgcct
    22201 gctacctggg tgagcaactg ataggtgcgt tctgccgcgc gaaaatcagg aagattgagg
    22261 ctgatatctg ccagaaccac ccctaccagg gtcaccagcg gcaggtcaag ccccttggcg
    22321 agcatttgtg taccgataag cacgtctgcc cggtgctgga taaaatggtc cagaatgaga
    22381 tcgtgggctc cccggtaacg ggcagtgtcg gcgtcccagc gcagcaggcg cgcggatggg
    22441 aattggccct gtaaatactt ctccaggctc tctgttccca tcccaaattg gcggatgttc
    22501 atcccgccac aggaagggca ttgcgaaggc agcgcgcggc ggtaattgca ggtatggcaa
    22561 atcaactggt tttcatcagc gtgccaggta agctgagttt cacaacgcgg acagcgtaat
    22621 acaaaaccgc aatcacggca aaaaacatac gaggcgctgc cgcggcggtt gaggaaaagg
    22681 atcgcctgtt cattacgctg cagcacttcc tccagtgcgg tgtgtaacga ttggctgatg
    22741 gctgagcgat tgccagcgct taattccttc cgcatatcca ccacttcaac ctgcggcaga
    22801 tcggtaaagt tcggctgggt ggatggtggt tgttgaccgt ccggcgaggt atgtgccaaa
    22861 attcgctggg gaaggtggtg tatgggccag tggttctctg taaaccggta aagcgtttcc
    22921 acatctggcg tggcgctgcc cagtaacaga caggcgccgt tgatttgact gagcgaaagc
    22981 gcgcattgca cggtgtgata atgcggttgc gtgtcttcct ggcggtaaga gggatcgtgc
    23041 gcttcatcga tgacgatcaa cccggggtcg gagagcgggg aaaagagcgc gctgcgcgga
    23101 ccgatgacaa tgggcagcaa gccagcgcgg atacgacgcc aggtatcgta gcgctcgcca
    23161 ggcgaaagct tggaatggat cagaccaacc ttcccaggga agcgcgcgaa aaagcgcttc
    23221 accgtttgcg gggtgagcga aatctccggg acgaggatga aaacccctcg ccctttttcc
    23281 accacagcgg cagcagcccg caggtaaagc tcggtctttc ctgatccggt gacgccaagc
    23341 agcaggttgg gtacgggcgt ggatgggttc accagctgat gatgaatctc ctcccaaacc
    23401 ttttgttgat gattggtgag cacaggtgga tggatgagca cgggttgtag tttttccagc
    23461 gggtcgcgcc agatttccgt ctcaccgaga atgataagcc ccatctcggc aaggcggtta
    23521 aggtccgcgg aggaagctcc gctctccgca taaacccacg aaatatttac cgggattgcc
    23581 tgccgctcga ggaactcaag cgcggcaaca cggcgctggg ctgcttgtga gcttgcccga
    23641 ccgacaagaa taccaggatc atcgcgccat cctcgcgggg cgctgaacag cgcggtgcgc
    23701 acgacctttg ggcggacaga cggcgagggc agaacggatt gagtgactac cagctgcgcc
    23761 ttaaccaatg ctgacaggct ctcacgccaa ttcacccggg gcagcgccgt gtctacctgg
    23821 cgcccgcgca aggggccgcg cttttccagc agatcaagca ggcgctgctg cacagatgag
    23881 agtgcggttg ttcgcgcttg tttgctgcgc gttaccagag tgtcagcgtg ctggctcagc
    23941 ccgggcggca ccatcaggtc aaggcattgc ggcagggtgg ccaggttatc acgcgccatc
    24001 tgttcagcca ggcgcatttg ccaggaagtc agtactggtg tatcgtcaac aacagtcgcg
    24061 atgggacggg tttgatcaac agccggggtt tcaagcagag agatcacaac cccctgcgcg
    24121 gtttgcctgc caaatggcac ggtgaccaag ctgcccggtt gaatatgggg cgcgtactca
    24181 tcaggcacct ggtaatcaaa cagaccggtt aatggagcga tgttgacaga aacacgggcg
    24241 taattggaca ttgaaaaact tagcgggtga ccgcgagtgc gccataaccg accacgcggc
    24301 tggcatcgcc tgaggaatca gcagaggtac tgtggtgcag cagcgtaacc ctggtagcgc
    24361 cgagctcttt ttccgtccac aacatcaaag ccaccgcacc tgcgccgcag gcaaaccctt
    24421 cgccgcagcg ctccgctctc agcacaccct caggtgaaaa agcgctgatt tgttgcagca
    24481 tgctggtgtc cagttgaatt gcttcactct gagtatagaa atgtgaaagg tcggtactgg
    24541 ctaccagcag cgcattctgt tctttgagtg caggagcgat tgccgccgcc gcagccgtta
    24601 aaagagccgg gtcctgactg cgcatcatga ttggtaccag gctaaagggt gcgcttaacg
    24661 ccacctgcag gaagggaagc tggatttcaa gcgaatgctc acggtcattg gcgatgggct
    24721 gcaggatttc gcccgttgct ttggcgatgt tcgcagaaca ctgttctacc agatgctggt
    24781 taatgggcac ctcgccaagg ggggtggcat aggcaaggtg agagctggtg agcagctggc
    24841 tgggcaggta atcatgcagg ggtgagaaaa caacacaaac atcaaacgcg cgccctttga
    24901 cacaacggta ggcatgccct gcggttgcgc cggaataaaa atacccggcg tgaggagcga
    24961 taagacctgt gacttcaccc ggcagcgctg gcggtgttgc tgcgtcaatg tacgctttga
    25021 tctcattacg cagtatatgc gggttgtctg agtaccaggc accggcgatt ggtgaacggc
    25081 ggatgggggg atgggggttc atacacacct cgctgtctat attatacaat cgcgcgccat
    25141 aaaaaaaccg gtcacgtatt ggtgaccggg aagggaggtg agtaccatta ttggtcatcg
    25201 cgttcgcgca aaggtgggaa gagcagtacc tcgcggatat ttttctgatc ggtcaacagc
    25261 atcaccaggc ggtcgatacc catgccgaac ccgccctggg gcggcatgcc ataagacatg
    25321 gccttaaggt aatcttcatc catggggtgc tgttcttcat cgtccgcttt gtaatcacgg
    25381 cccatttcga gaaaacgctt ttcttgctcc agcgggtcgt ttagttctgt aaatgcgttg
    25441 cacagttcca ttccgcacac aaaaccctca aagcgttcca cggtcgatgg gtcacccggc
    25501 aggttcttgg ccaatgggga aatatctcgt gggtaatcaa tcataaaggt cggctgcacc
    25561 agggtgggct ctacaaattc ttccagcagc tgttcgataa gcttgccccg agcttccccg
    25621 ggtttgacct tcatgtcaag tgaaaccatc atttcttcaa gtgcttcggc ggtggggtaa
    25681 agtgagatgt caatattggc tttctctatc aaactttggc gcaggttcac ccgttgccag
    25741 ggcgggttga aatccagttc tacgccgtcg taagtcaatt tgcgggtgcc aaaaaccgaa
    25801 tcgcaaacat aggcgatcat ctcctcagtc atttccatta ccttgaggta gtctgcgtag
    25861 gcccaataaa actccagttg ggtaaactct gggttatgct tataagacac tccttcattg
    25921 cggaagtcgc ggccgatttc atacacccgg tcaaaccctc ccacggtaag gcgcttgagg
    25981 taaagctcaa aagagatgcg caggtaaaga tcctgcttta actggttatg atgcgtaacg
    26041 aagggcttgg ctgctgctcc gccatagata ggctgtaaga tgggggtttc aacctccaga
    26101 aaaccacggc tgtcgaggaa ctggcgcagc gcgcgcacca cggctgcgcg cgtgcggaag
    26161 atctggcgaa cctcctggtt cacagccagg tccgcatagc gctgacggta acgggtttcg
    26221 ggatctgaaa gggtggcatg gcgcaccacc tgcccatcca ccacttcatc tttagccgcg
    26281 ggcagggggg tgatggcttt cgccagcatt ttaaagttgc gcaccagcag gcttacctct
    26341 cctgtacgcg tacgcaccag gtctccgccg gcttgaatga aatcacccag gtcaaagtgg
    26401 tccttaaacc gctgcatttc ggcttccccc agttcgttta cccggaagaa aagttgaatg
    26461 cgaccgctgc catcttcaat gtgggcgaat gccagcttac ccatcaggcg caccgcgcgc
    26521 agcctgccgc ccagcacggt ggtcagcgtt tctggttttc cagccttctc ggcattttca
    26581 aaggctttga tcgcgtctgc tgttgtgctg gttacctcgg tgcgcgttgg atagggttcg
    26641 aaaccgctat cgcgcatttg ctggattttt tcacaccgta ttctttcaag ctcgttcagt
    26701 tccatggttt gttccttttc aaatcaagaa gcccgccctt gtttgcgggg caggcctcgg
    26761 tttcttcaga gaacgaatcg ctctggtgtt gctgcggcgt agcacccggt tgattatagg
    26821 tccaccttga gcactttgac cgtgtaagca cctgcggggg cctcaatttg gaccttgtca
    26881 cccacgcgtt tacccaacag cgctttcccc attggagatt cgtttgaaat ttttccgagg
    26941 gttgggtcag cttctgctgc gcccacgata acgtactctt ctttttgtgt cgcgcctatc
    27001 tcctggatag ttacacggtt accgacctga acggtttctt tatcaatctc cgcctcattc
    27061 acaacacgag cggtggcgag aagaatttct aattccttga tcgaaccctc aacaaaagcc
    27121 tgttcgtttt ttgccgcttc aagctcggcg ttatccagta actctccgcc ttcaatggct
    27181 tcgtgcaatc tttgggcaat ctgttcgcgc ctggtagtgc gcaggtactc aagttcatcc
    27241 tgtagttttt gatatccttc ggtcgtcagg actggtgttg gcatatccat ttccttccca
    27301 tattggttcg tgatagggga attccctaag aagatgaaaa ataatttaga tagaaaaaaa
    27361 ccactccggc gggagcgatt taatattgag agaaaattgt ctctttcctc tctgaagtag
    27421 ccatactata acataaaaaa agaacctttg caagcatt
//
LOCUS       DAOSPM010000012        27350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1758_length_27350_cov_14.697747, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000012 DAOSPM010000000
VERSION     DAOSPM010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 27350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27350
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1758_length_27350_cov_14.697747"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PLW25_02225"
     CDS             <1..1239
                     /locus_tag="PLW25_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOS53335.1"
                     /translation="LKNGQARSLLEGVPLAIKDELDQVPYPTTVGTQFLGARPAAKDA
                     TVTARLRAAGAVLVGKTNMHEIGIATNGENVHHGRIANPYDLQHDPGGSSSGSGAAVA
                     AGIVPAALGADGGGSIRVPASLNGVVGLKPTFGRVSESGAAPLCWSVAHIGPLGASVE
                     DVALIYQVIAGPDPAEPNTLQQPPPTLRGWHTPDLKGVRIGIYADWFNHATQEVVALN
                     YAVVSAFEKAGAQLIEVTIAELDAMRVAHAITILSEMAACMRPYPQHWKDFAASTKLN
                     LVLGQELTAYDYIQAQRMRTRALNIFAEIYREVDVILTPGTAMTAPIVPPQALSAGWS
                     DLGTDTEMMRFVYPGNLTGLPAITFPVGYDANGMPVSMQAMGRHWEEHLLLRIAYNAE
                     LYTTRKLPQTYYGSRILGIS"
     gene            complement(1415..2860)
                     /locus_tag="PLW25_02230"
     CDS             complement(1415..2860)
                     /locus_tag="PLW25_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOS53336.1"
                     /translation="MPILTPGRRWQPIFYPFKKDKLGNRLLIRLEMLYKGPLWGCRQC
                     GNCVLQETAFICPMECPKGLRNGPCGGSTPEYCYVDETRPCIWYKIYERAFQSGREEL
                     LMEVLPPLDWDLVGGEQLGRLAGQVKKNGVLRVFTGLVSLDKAKRQRTWDGIFRPVRQ
                     PAWWRGDSVYHAPKYEGDASELERRLKAGEFVITAEVQPPQTASTNRLKQNIEMVSKH
                     ASAVNFTDGASATPRMSSWACSAIASQMGVEAVLQIAARDITRVSLQGTALGAAALGI
                     KNILCVTGDSNRLSPSPRGRMDIYDLDAIQMLWILREMRDEGRYLDERNIKYPPKYFL
                     GAVASPSASEVKFQALREHKKVNAGAQFFQTNILFDLGRFEEWLNALAARNILDKVYI
                     LAGISPLRSLRSAQFLNKEVPGVRVPDHILRRMEKADAAGNTQEEGIQVALELIEKLR
                     QYKEVNGLHLMTVGWEEIIPRLVHDAGLSQS"
     gene            complement(2981..>4567)
                     /locus_tag="PLW25_02235"
     CDS             complement(2981..>4567)
                     /locus_tag="PLW25_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOS53337.1"
                     /translation="AALAAKEAGLDPIIIESTGKLGGSSAMSGGGAWIPNNPLMRRAG
                     VFDSYEEARKYMDLTIGDVGPASSPERREAYLREGPQMVEWLQSLGMRLHYGKGYADY
                     YPELPGGSPLGRLVEPEPFDLKKLGQWKDKLNISISIPVYTLDGVKIALSMRSLSTFL
                     HTAYVIGIRTMGSMLLGKKLAGIGGALIGQLTFLALQRNIPIWLNTPLVELISEGGEI
                     KGVIVEKEGKRTELRARAVILAAGGFPHNNEMRQKYHPHPITTDWTVAHKGNIGTAIQ
                     AGMAVGAATALMDDAWWGPCFIDSKGVSQFMIWERSFPYSIIVDKSGKRFMNESASYV
                     DCGHWQYERNKEVPAIPAYLIFDQHHHRYYPLGVGLPGAEPKEYFESGMLTKANTLAE
                     LAQKIGVDPQGLEETVTRFNQFALNGKDEDFKRGDSAYDRIYSDPKVKPNPNLAPLTQ
                     PPFYSVKVWPGDLGTKGGLLTNEHAQVLREDGSIIKGLYAAGNTSASVMGHTYPGPGS
                     TIGPAMVFGMLAGRHAATLE"
     assembly_gap    4568..4667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4679..5032)
                     /gene="trxA"
                     /locus_tag="PLW25_02240"
     CDS             complement(4679..5032)
                     /gene="trxA"
                     /locus_tag="PLW25_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOS53338.1"
                     /translation="MNEPVHVTDAAFEKAVLQSTLPVLVDFWAPWCGPCRMVAPILDK
                     IAKDYEGKVVVAKVNTDENNQWAMKYGVQGIPTMLFVANGKVVHQQVGALPEEMLRGT
                     LDQFLEVVAEDKKAG"
     gene            complement(5066..6022)
                     /locus_tag="PLW25_02245"
     CDS             complement(5066..6022)
                     /locus_tag="PLW25_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS53339.1"
                     /translation="MVNEGQQYEVIIIGGGPAGATAAIYTARAGLKTLVLDKGLTAGA
                     LGITSKIANYPGLNAEVSGAQLLENMRAQARSFGAEFITDKVIGVNLLAEQKSVYGNN
                     GTYTGKAVIIATGSMGRGNRIKGEDELLGRGVSYCATCDAAFFRGQDVIVAGNSDEAI
                     EEALFLTRFVNRVHFLCPTPELKAPQPLIDEISANPKVQLYKGAALKEVIGTGKVQAV
                     RFTQRGLPEQELAVSGAFIYLQGGKPITDFLVGQLKLNDGGCLLVDEEYQTSIPGVFA
                     VGDILCNHIKQAVVAAADGAIAGMAAEKTLRGRKQMTVDWSK"
     gene            complement(6246..7787)
                     /locus_tag="PLW25_02250"
     CDS             complement(6246..7787)
                     /locus_tag="PLW25_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOS53340.1"
                     /translation="MDEKTLNGHINHLRETFKSGITQPLAWRRRQLCMLNKMLKERSQ
                     EFETALSTDLRKSPYETYLTEIGLLQMEIDEALRELSNWSKPKSVPTPVFLHPARSYT
                     TFEPLGVMLIIGAWNYPLLLTIGPLVYAIAAGNAAVIKPPRTASAAFMAMANLLPLYL
                     DTDAFFVIGDDTPNELILEQRFDKIFFTGSSATARIVMTAAAKHLTPVTLELGGKSPA
                     LVDRTADLKVAARRIAQGKFFNAGQTCVAPDYVLADEGILDAFVAELRSALYDFFGPS
                     PRESADFSRIINQKQFDQLLQYLSDGEVVVGGEWDREARYIAPTILKNVQLDAPVMQA
                     EIFGPILPVLPVKDMDAAIHFVQERDKPLALYIFSKDQATIDHVLSRTTSGGACVNEA
                     VNHVVVTGLPFGGVGNSGMGKYHGEWGFRELSNARAVLDHGTGFDPLLRYPPYNTAKM
                     AKLKTLMEMRTPAFLDGAMRLLIGKFGNLILRLVRPKAVEHSPLSSASAKKPQPAVQP
                     DCVET"
     gene            complement(8047..9690)
                     /locus_tag="PLW25_02255"
     CDS             complement(8047..9690)
                     /locus_tag="PLW25_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOS53341.1"
                     /translation="MTTLGNMEKWTLRFAVVALLFLALSGFEGMLMRSQQYNIDSLHG
                     MEALLNNLRPGDVEPTTAELYYAMLSAHPIVGIYGFVYMAVFGAFYFLVPYLIQKPVK
                     HPKLVVANFWLQIIGVMTCWAAGFFGLFNAMYTLYWPLPVAFDRVPVVGSIFFMLGAA
                     AIMVNILLFSYNIFKTVLSKNGEGSYTMGQFLRAAFGIPRLLKLFGKEDKTAVNLDYN
                     GLPPFIVAVARGSIDTVINAIVLLSAGVLILVYGLFALFGHPLNPLAVDALIYKNIYW
                     WGLDMVADGNALIYTAGVWYLLVPLLTGRKLFGAGVVKTVIMADLLVSMGVWSHHLLG
                     DTSQPMWMKILSGQFITWGEYFTMGLTIFVSLMTIWKARPVKMSPAMKFTLGSMFGFV
                     MGGIAGLIQANVGLNMVFHNTQWVVALHAHTFLLTGVGTMLFSVIYTLVPLLTKLEFK
                     HPKLVDWHLWLWLIGSVSMAYAMGMAGSNGMIRRTLYTGGEFSTNTLAAFIGGSVVSI
                     GFVLFLINLVSTIGLKNVLSLVLPDRKTRQPDPLQAPAQ"
     gene            complement(9703..10119)
                     /locus_tag="PLW25_02260"
     CDS             complement(9703..10119)
                     /locus_tag="PLW25_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53342.1"
                     /translation="MEKAEKRWLYVLIIVFVIVNVVTLSSLIPWQSWQLWQNPEPAQV
                     VKVAYNDYTITMDTPVQIKAGEFVQFSATSADVTYGFGVFREDNSMVFQMQVLPGREN
                     QIIWKFDETGTFDVRSTEYSGPKHSDMVVRDAIVVK"
     gene            complement(10658..12034)
                     /locus_tag="PLW25_02265"
     CDS             complement(10658..12034)
                     /locus_tag="PLW25_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOS53343.1"
                     /translation="MTEKVTTHTKLLYGIADSGIAMLTSIVQFFLLFYYTDVALLDPA
                     MVGTALMVGKLTWDAINDVLFGYISDRTRSRWGRRRPYLLLLAIPLALSTWLMFSIPA
                     GLTGIPAFLIVLFTFLLFDSVHTFIAVAYTAITPELTSDYDERTSVTTVREVFTVVGY
                     ILGAALTTVVAGLYQKNLGFSLQGSYSAMGLTFGIYAAVVVLITAFGVKGKPASEIQP
                     AKMPAVKAFLVTFKNKPFVHLVTAFLIANVSFTLLTTLLPYYLKYQVQMEDQMPFVMI
                     SLLVTIGVFLYPMKMVVDRIGKGKGYALGLGIASLAILSIFFFPKGPTPLVYVASIVA
                     GMGLSAQWVCPWSMVPDVIEIDEIATGERREGIYYGMWNFITKLANAFAIAAAGWMLA
                     SFKYIPDAPQSDFTLLGIRILFSLVPVLMFAITIPLLIKYPITRESHARLVAELRGKI
                     SAGKETTT"
     gene            complement(12072..13772)
                     /locus_tag="PLW25_02270"
     CDS             complement(12072..13772)
                     /locus_tag="PLW25_02270"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOS53344.1"
                     /translation="MTETIKIFVLDVSHMSYAEQVTAACLQGLANRDQPRLFLDYGIY
                     DDPESRKTNQDALGEDLWVSKFRDAMGRQDEHNLNAYQKLYPIHVTKLNDLSSAVHQF
                     QDLCRGFVVWDPQQEDTVNLAVMFCSVENWLPIAPDLVEWSKQFGLKIEKDLRGCWQD
                     HVALHAWALEQLFARCRPDRVANIEPHWHRPELLDYVVKEKIFTCNLSSKGSGRAFNF
                     GWKLLTLCLGGPTWLRNALYNVGLFRWVKNFALQKMSSDPETGLLISLLRKVARHPEG
                     IIFGWHTNRDDEFAFLALLSAIGLRLVPSHLAANFSFHSALPDPGPLKQFHVSEADVA
                     LEPEKDYLTFTYSDGDQLLLMSTAQLGGWRRKERGLVPFNWEMQPLLAELAPALLGLF
                     YSTLTPNDCLIAGPSGAGYIIPPLHDNLPEYLRRSAEVCAKADINVITSYYPDPPSKV
                     IQQHLQAPDNLIGYLSGYFYLKHRPVKFGHNKVFLCNLWPHLSQIKDSSDEVMAGVKK
                     ILDTPSSTPRFIGVHLFAYCTTVSDVYDFVRTLDPSKVKVVRADEFLLAAKKYSLQSK
                     "
     gene            complement(13781..15112)
                     /locus_tag="PLW25_02275"
     CDS             complement(13781..15112)
                     /locus_tag="PLW25_02275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOS53345.1"
                     /translation="MSEHWNADVVIVGGGPTGSYLGWKLAQAGSDVLILETLPLEKVG
                     AHIEVIHLDQVRFDEFEIPHPLPPERMHLVPANKVWAVDKSGYFEVRYPFYVVNMPAF
                     VQRLHGYARESGGRVVGGAQVKEVIMEDGYCRGVRGTFNQQPFEARARIVVDASGLIS
                     AVRTKLPDDFGVENCPVPAEQTFYCTLELRDEIPAGYPVDNNSYLGSPGYWNKSYGDG
                     AVLGIIAPASVKNAWTALTQWRQATFGEPGKLVAKRAGCSPYRRSPNSYVGNGFVGVG
                     DCVYQNKAFSGEGITSGFTACKIASEVMVKALKNGDVSREGLWEYNVRYFHGQGAKFA
                     GVMMFLFPVNGLPNHEIDYLNRHNIIFCQDDYEYMNLNYELNLPAERWAAICEQLSDG
                     MKKGLFSENSLRLMNALYQKAGQVSAHYRAYPVNPSGIEAWSKVTREYWGY"
     gene            complement(15266..16027)
                     /locus_tag="PLW25_02280"
     CDS             complement(15266..16027)
                     /locus_tag="PLW25_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOS53346.1"
                     /translation="MKNRILFSLLVLALVLSACGGNGSAVTPSLTGTHWVLEQINGKP
                     VIENVIPTLSFRGDQEVGGNASCNNFNGTYTGKDGALTIGPLATTMMACLEPAGLMEQ
                     EAAYLKALESAVEYRIEKGKLLIMDSAKAVVLTFAAQDMSLEGKTWNLTSFNDGQSLV
                     SLVPDTQITAEFKDGALSGSSGCNSYHGLFHQQEQKLSFDTFAVTMMACPEEGVMEQE
                     AAYLSSLAKVTHFSVEGNRLFLFDANDLIMAQFSQ"
     gene            complement(16221..17423)
                     /gene="larC"
                     /locus_tag="PLW25_02285"
     CDS             complement(16221..17423)
                     /gene="larC"
                     /locus_tag="PLW25_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOS53347.1"
                     /translation="MTCLYCDCFSGISGDMFLGALLDAGLPVETLSTQLKKLNLPEFQ
                     EAGKTKVFKGSLQATLLKLSIHQHAEDDQHHPHTHHRHFRDICQLIESSGLSEQVQQT
                     SKKIFGKLAEAEARVHGSTIEEVHFHEVGAVDSILDIVGAAIGLEYFGVQQVFSSALP
                     LGSGQVNTQHGLLPLPAPATMELIRMANAPVTGSKATSELVTPTGAAILSALAVFRQP
                     EMKIQRVGIGAGQRDLEWPNILRVFIGEAGDDFCTHLEIETNIDDMNPQVYGHVITKL
                     FQAGALDVFFTPIFMKKNRPATKISVIARKEDEQNISDLILRETSTLGVRVKPIWRHE
                     SGREFRNVETHYGIVPVKLKVSGDDILQAAPEFDRCVQLAEQNQVPVLQVLKEVEAQV
                     TAHFKGQC"
     gene            complement(17420..18274)
                     /gene="larE"
                     /locus_tag="PLW25_02290"
     CDS             complement(17420..18274)
                     /gene="larE"
                     /locus_tag="PLW25_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOS53348.1"
                     /translation="MNPLEQANQRLENAGITHPLKAKWQSLITELSSYPSAVIAYSGG
                     VDSSFLAYAAAQAMGGPMIAVTVFSPLEPAGLQQDVFRFVEQYGIKHDFLTHDPLQNA
                     KFQNNPPERCYLCKLGILNILWEYARDNHYAVLLEGQNADDQHEYRPGRQAVKETGTF
                     SPLAQNALTKAEIRWLARALGLPIWNQPSSPCLATRIPYGMTITEERLDQIASAENYL
                     HQRGFPVVRVRHLQHYASIEVEPDHLAKLLEMREDVVSYFKEIGFHHVALDLQGYRSG
                     SMDEGLSL"
     gene            complement(18261..19040)
                     /gene="larB"
                     /locus_tag="PLW25_02295"
     CDS             complement(18261..19040)
                     /gene="larB"
                     /locus_tag="PLW25_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOS53349.1"
                     /translation="MDQETLRKLLENLIKGELTVPQVIDQLKTLPYEGLNDYAKLDHH
                     RPLRTGLPEVIFAQGKTPQQVLDIFTRLQKSHHQVLATRVSPGLYAEIQDHLSGVTYD
                     STARLLFTSPDTRQVKRPGIVVLTAGTSDIPVAEEAAITAELMGNKVERIYDVGVAGL
                     HRLISHLPVLQKARVVITVAGMEGALPSVVAGLVSAPLIAVPTSVGYGASFQGLAALL
                     AMLNSCASGVAVVNIDNGFGAGCMAAKINRLSSNESGNEPS"
     gene            complement(19116..19820)
                     /locus_tag="PLW25_02300"
     CDS             complement(19116..19820)
                     /locus_tag="PLW25_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53350.1"
                     /translation="MEPVIEIRNLSFSYPDGKQALAGINLLVQPGEKVALVGANGAGK
                     STLLYHLNGIFTGSGMIRVNQLELNKNNLPRVRAMVGLVFQNPDDQLFSPTVYEDVAY
                     GPTYQGFDPHIVREKVALALKAVHMEDYTLRNPYHLSSGEKKRIAIATVLSMQPQILA
                     FDEPTAGLDPRARRELIDLLLELQQTMIIATHDMNMVEQLTPRTVLLNHGKIAADGPS
                     LDILRNDRLLMTNGLI"
     gene            complement(19824..20675)
                     /gene="cbiQ"
                     /locus_tag="PLW25_02305"
     CDS             complement(19824..20675)
                     /gene="cbiQ"
                     /locus_tag="PLW25_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOS53351.1"
                     /translation="MASPALKSAHPLKTTPKGRRLRELYTPNHSAVHRIDARVKVIFT
                     LAFIVFLNLTPSGAWPAYILFFTWMFSLSLSSRLGIAFVLKRSLLALPFVLAALPLVF
                     TGPAPLGQIPVLNTPSLTYSVVGFYRFASIAIKAWVSVMAAVLLAATTRFPDLLTAFS
                     QLKVPALFLTIIGLMWRYLFVISDEVTRMLRARTSRSATAPGSNRSGGSLFWRARVTG
                     GMAGNLFLRSIERSDRVYAAMLSRGYTGQPAAQKTTRLQKKDLLVLAAGVILLVILWL
                     LGVITGG"
     gene            complement(20620..21588)
                     /locus_tag="PLW25_02310"
     CDS             complement(20620..21588)
                     /locus_tag="PLW25_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOS53352.1"
                     /translation="MLFSPINPMHIPDGFLSVLLSIVLWVISVVLIGIALRKTGQTIG
                     ERQVPLMGVLAAAIFAGQMLNFSVTGGTSGHLLGAAIAAILLGPWPAVLVMTSVVAVQ
                     ALLFQDGGILALGANLFNMAIIGPFVAYALFTLLMKLFKKQTWGLFVAGFVAAWSSIF
                     IASLACALQLALSGTSPANIAVPAMGAIHALIGIGEGLITIGALAFIFAARKDLLKAD
                     ANKPARNIGVVVGGLLLSLVLVVLSPLASSHPDGLEWVAEKHGFIEAAREAFYNIVPD
                     YSMPGISNPALATIVAGILGAVIVFGVAYGIARAEKRPSAQDDAQR"
     assembly_gap    21854..21953
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21954..>23131
                     /locus_tag="PLW25_02315"
     CDS             <21954..>23131
                     /locus_tag="PLW25_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOS53353.1"
                     /translation="VDAQQSLAESSFQKPYDALTLPEQQAVQLQVTDLLKENRYDPES
                     GVLILTAAEAASFQRQIQVWTDYFASTTSSSGLKQDLISHPEEIKQLTAFFAWSAWAS
                     VAQRPDKPYSYTNNFPYDPSVGNTATADTVLWSALSLIALLGGLGLVLFVVGRWNFLG
                     WRGKQEGELEEPKPIASEIATPAQRATIKYFAVVVLLLLLQVAMGALVAHYRADPSTF
                     YGLDLSKILPSNLDRSWHLQIAVFWIATSYVAGALFLAPILSKKDPKHQVAGINILFG
                     ALVLVVAGSLIGEWLGINNKMGNLWFWLGHQGWEYLELGRIWQILLAIGLLLWLFLLV
                     RALKNASQNPDFKQMASLFIITAIGIPIFYVPAMFINSMTNYAVVETWRFWIIHLWVE
                     G"
     assembly_gap    23132..23231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23232..23789
                     /locus_tag="PLW25_02320"
     CDS             <23232..23789
                     /locus_tag="PLW25_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOS53354.1"
                     /translation="ISQGKWEIVKRHKWTFYFLMAVGFWNFLGAGVFGFLINLPIVSY
                     YEVGTTLTPNHGHTSFMGVFGMLSLALIVFGLRQVLDDQKWKKVDKFVGVGFWGLNIG
                     LLLMSLLSLFPGGVLQLLDVLKNGYWHARSQAYMGQPLVKTIEWIRMGPDLIFVLAGC
                     IPLAIAVLFTLWQVIKHKKSADANH"
     gene            24226..24813
                     /locus_tag="PLW25_02325"
     CDS             24226..24813
                     /locus_tag="PLW25_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOS53355.1"
                     /translation="MANKPKPEHENANRILATAWRLFQQKGYKGVTIDEICSGCGLTK
                     PTLYYYYRDKEGLFVSVLQHQLALFNRVLTQPGNIEQQLQRLAVAILENFQIEYSVLM
                     RDRAHIKKPENQAAVREAFRSGLFNPLLLLMETGLKNGDLQARNPEVLTLAFLGIINN
                     FINRGAESGTSTAELAAELTQLFLQGAKGQRAGST"
     gene            24934..>25125
                     /locus_tag="PLW25_02330"
     CDS             24934..>25125
                     /locus_tag="PLW25_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53356.1"
                     /translation="MFEQQRSIEDRTKNSWWSYLKPTLPKRWLLIVAGVMWSAVGIML
                     LNYAVTWLTLSFSIPAILLG"
     assembly_gap    25126..25225
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25226..25434)
                     /locus_tag="PLW25_02335"
     CDS             complement(<25226..25434)
                     /locus_tag="PLW25_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOS53357.1"
                     /translation="MVKIKRVYERPQESDGVRFLVERLWPRGMKKEELKIDSWVKDVA
                     PSTDLRRWFGHDPLKWDEFQKRYLA"
     gene            complement(25773..>27350)
                     /locus_tag="PLW25_02340"
     CDS             complement(25773..>27350)
                     /locus_tag="PLW25_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53358.1"
                     /translation="YWSNKLGISSTSKWWLETSAEYMVMLYEPKYIDQNLLSYGTTSL
                     GADKRTPFQFAPNQWNDDLYLHAQLLKVFTCDNPSACPISPQQFVNAINNGTFPIDSS
                     AVEKISANLDDFARYLLGKSPQKANSMIYLLNSVKNGTGYGESVTPVDNKGDLNFKKT
                     GYEPQMKQTSGGTGTELDIQAVIEKGGVYPLMLETPRNAELAGYPVEIEVSPGCPFYY
                     TEEGGEIQYNDGTAKTVIGPLHSKTGLSGIRIVAVATDGAKTFTAKIRPLDISGDWIF
                     VFDSIVSNNMVCDSSGNESNDNNDPTTPEEMAQLTSYINSYIPAIPGEYVKDAGANTY
                     TWTLSPGGDLTFGGDTAVVVNGMALLDSKGITVQTFVSIPQPESRLPGNKAIPVAMAS
                     TCFFVFPLLFRKNKRVFLISIISAMLLISGCSAFFLMYGDLTTSTVVDKLLPASPENT
                     VKVFGESASTQVNELTPKYIASGITTADVNWTIGGGTVTIEGESSSTTVCTGKIVYKV
                     NGVLLNDGVITDFPGSE"
BASE COUNT         6362 a         7116 c         7116 g         6356 t
ORIGIN      
        1 cttaagaacg gtcaggcgcg cagcctgctg gagggcgtgc cgctggcgat caaagacgaa
       61 ctggaccagg tgccttatcc caccactgtg ggcacccaat tcctcggcgc acggcctgcc
      121 gcgaaagacg caactgtgac cgcgcgcctg cgcgcggccg gtgctgtatt ggtgggtaaa
      181 accaatatgc atgaaattgg cattgccaca aacggagaaa acgtgcacca cgggcgcatc
      241 gccaacccgt atgacctgca gcatgatccg ggaggttcct ccagcggttc gggcgcggcg
      301 gttgcagcgg gaattgtgcc agccgcgctg ggcgctgacg gcggcggttc gatacgtgtg
      361 ccggccagcc ttaacggtgt ggtgggactg aaacccactt ttggcagggt gagcgagagt
      421 ggcgccgcgc cgctatgctg gagcgtcgca catatcggcc cgctgggcgc gtctgtggag
      481 gatgtggccc taatctacca ggtcatcgct ggcccagacc cggcggagcc caacacgctg
      541 caacaacctc cgcccaccct gcgtggctgg catacacccg acctgaaagg cgtgcgcatc
      601 ggcatatacg ctgactggtt caaccacgcc acccaggagg ttgtggcgtt gaattacgcc
      661 gtggtatcag cgtttgagaa agccggcgcg cagttgatag aagtgaccat cgcggagctt
      721 gacgcgatgc gtgtagctca cgccattacc atattaagtg agatggccgc ctgcatgcgg
      781 ccttacccgc agcactggaa agatttcgcc gccagtacga agctgaatct ggtgttgggg
      841 caggagttga ccgcttacga ttatatccag gcgcagcgta tgcgcacccg cgccttgaac
      901 atcttcgccg agatataccg tgaggtggat gtcatcctca ctcctggcac agcgatgacc
      961 gcgccaatcg ttcctcctca ggcgctcagc gcgggctggt cggatctggg cacggacact
     1021 gaaatgatgc gctttgttta ccctggcaac ctcactggtt tgccggcgat cactttcccg
     1081 gtgggttatg atgccaacgg gatgccggtg agcatgcagg cgatgggtcg gcactgggaa
     1141 gagcacctgt tgctgcgtat agcctataac gcggagctgt acaccacgcg caaactcccg
     1201 caaacctatt acggctcgcg catactgggt atttcctgaa ttccagggca aataatgaag
     1261 atcccgcgat tgactcgcgg gatgcttgat tcccggttgc ggtcgaccgc gaagtgttag
     1321 attcttttaa gaatgtgcgg gcgaacagcg ctgctctgat ctggccacga tatccttctg
     1381 tcttcaagca cgcctgagat gatctctccg atcgttacga ttgtgagagg cccgcgtcat
     1441 gaaccagcct gggtatgatt tcctcccagc ccacggtcat gaggtgcaag ccgttcacct
     1501 ctttgtactg acggagcttt tcaatcaatt caagcgcaac ctgaatgccc tcttcctggg
     1561 tgttgccagc cgcgtctgct ttttccatgc gccggaggat atggtcaggc acacgcacac
     1621 cagggacttc tttgttcaag aattgggcgc tgcgcagcga gcgtaaggga gaaattcccg
     1681 ccaggatata caccttgtca agaatatttc gcgctgccaa ggcgtttaac cattcttcaa
     1741 aacgacccag gtcaaataga atattggtct gaaagaactg cgcgccagcg ttgaccttct
     1801 tatgttcccg cagcgcttga aatttgacct cagatgccga gggtgaagcc acagcaccga
     1861 ggaaatattt cggcggatac ttgatattgc gttcatccag gtacctgcct tcgtcccgca
     1921 tttcgcgcaa tatccacaac atctggatcg cgtccaggtc gtatatatcc attctgccgc
     1981 gcggagaggg tgacaggcgg ttgctatctc cggtgacaca aaggatgttc ttaatgccca
     2041 gggccgctgc ccccagtgct gtgccctgca ggctcacacg ggttatatcg cgcgccgcaa
     2101 tttgcaacac cgcttccacg cccatctgcg acgcgatggc tgagcatgcc cacgaagaca
     2161 tgcgcggcgt ggctgaggcg ccatcggtga aattcaccgc gctggcgtgt ttgctgacca
     2221 tttcgatgtt ctgtttaagc cggttggtag aagccgtttg cggcggctga acctcggcgg
     2281 tgatcacaaa ttcgcctgct ttcaaccggc gttccagctc tgaagcatcc ccctcgtatt
     2341 ttggcgcatg gtacacagaa tcgccgcgcc accaggcggg ctgacgcacc ggacggaaaa
     2401 taccatccca ggtgcgctgc cgtttggctt tatcgagtga aaccaggccg gtgaacaccc
     2461 gcagcactcc gttctttttt acctggccgg caaggcgccc caactgttct ccgcccacca
     2521 ggtcccaatc cagcggcggc agcacctcca tcagcagctc ttcacgaccg ctttggaaag
     2581 cgcgttcgta gattttatac cagatgcatg ggcgggtttc atccacgtag cagtactcgg
     2641 gtgtggaacc gccgcatggg ccattacgca gccccttcgg gcattccatc gggcatatga
     2701 aggctgtttc ctgaagcacg caattaccgc actgccggca gccccataag gggcctttgt
     2761 acagcatctc caggcggatg agcagccggt tgcccagttt atctttttta aacgggtaaa
     2821 aaatcggctg ccagcggcgt ccaggggtca aaatgggcat agtggatttc ctttaccgtt
     2881 ctcctgatta tattggggtt tccagtcacg tcaagaagaa aagaaggagc atgccacgca
     2941 tgctccaatg tgcgcacatc acccttattg gcgctggcgt ttattctaat gtggcggcgt
     3001 gcctgccggc gagcatgccg aaaaccatgg ccgggccgat ggtggaaccg ggtcctggat
     3061 aggtgtgccc catgaccgaa gcggaagtgt tgcctgccgc gtacaggccc ttgatgatgc
     3121 tgccatcctc tctcagtacc tgcgcgtgtt cattggtaag caggccgccc ttggtgccca
     3181 ggtctccggg ccacactttg actgaataga atggtggttg cgtcagcggc gccaggttgg
     3241 ggttgggttt gaccttggga tcgctgtaaa tacgatcgta agcgctgtcc ccgcgtttga
     3301 aatcctcatc ctttccattc agcgcgaact gattgaagcg agtcaccgtt tcttcaagcc
     3361 cctgcgggtc gacgccaatc ttctgcgcca gctcggccag cgtgtttgct ttggtcagca
     3421 tcccagactc aaagtactct ttgggttccg cgcctggcag ccccaccccc agcgggtagt
     3481 agcggtgatg atgctggtcg aagatgaggt acgcggggat ggcaggaact tccttattgc
     3541 gctcgtactg ccagtgacca caatcgacat aagaagcaga ctcgttcata aagcgcttgc
     3601 cggatttatc tacaatgatg gaatagggga aggaacgctc ccagatcatg aactgcgata
     3661 cgcccttgct gtcgataaag cagggtcccc accaggcatc atccatcaac gcggtcgctg
     3721 cgcccacggc catgccagcc tggatggcgg taccgatatt gcctttgtgc gcgactgtcc
     3781 agtcagtggt gatggggtgt gggtggtact tctggcgcat ttcgttattg tgtgggaatc
     3841 cacctgccgc caggatgacc gctctggcgc gcaattcagt gcgcttgccc tctttttcga
     3901 caatcacgcc tttgatctcg ccgccttcac tgatgagttc gaccaggggg gtattcaacc
     3961 agatgggaat gttgcgctgc agggccagga atgtgagctg cccaatcaac gcgccgccga
     4021 tacccgcgag cttcttgccc agaagcatgc tgcccatggt gcggatgcca atcacataag
     4081 cggtgtgcaa aaaagtggag agcgaacgca tggaaagcgc gatcttgacg ccatccagcg
     4141 tgtaaactgg gatcgatatg gagatattga gcttgtcttt ccactggccc agtttcttca
     4201 ggtcaaaagg ttctggttcc accagccgac caaggggcga accgccgggc agctcggggt
     4261 agtaatctgc gtagcccttg ccgtagtgca ggcgcatacc caggctctgg agccactcca
     4321 ccatttgcgg tccttcgcgc agatacgcct cacgcctttc aggcgagctg gctggcccta
     4381 catcgccgat ggtcaggtcc atatatttgc gggcctcttc gtacgagtca aagactccgg
     4441 ctctgcgcat cagcgggttg ttggggatcc acgcccctcc gccagacatg gcggatgaac
     4501 ctcccagttt gccggtgctc tcgatgatga tgggatcaag accggcttct ttggcagcca
     4561 gcgctgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngga ttgcctgctt
     4681 acccggcttt tttatcctcg gcgacgactt caaggaactg gtccagcgtg ccccgtagca
     4741 tctcttcggg caaagcgccg acctgctgat gcaccacttt accattggcg acaaaaagca
     4801 tggtagggat accctgcacg ccgtatttca tcgcccactg gttgttctca tctgtgttaa
     4861 ccttggcgac cacgaccttg ccttcgtaat ccttggcaat tttgtccagg atgggggcga
     4921 ccatgcggca gggaccacac cagggcgccc agaaatccac cagtaccggc agagtagatt
     4981 gtaaaacggc tttctcaaat gcagcatcgg taacatgtac aggttcgttc attttttttt
     5041 ctcctccagg ataggtaata ttaatctatt ttgaccaatc gacagtcatc tgtttgcgtc
     5101 cgcgcagggt tttctctgcc gccatcccgg cgatcgcccc atcagcagcc gcgaccaccg
     5161 cctgtttgat gtgattgcac aggatgtctc ccacagcgaa cacaccagga atactggttt
     5221 gatattcctc atctaccagc aggcaccccc cgtcgttgag ctttaactgc ccaaccagga
     5281 aatcggtgat cggttttccg ccttgcaggt aaatgaaggc gccgctcacg gcgagttctt
     5341 gttcaggcag ccctctttgg gtgaatctca cagcctgcac tttacccgta ccaatcactt
     5401 cttttagggc tgcccccttg tatagctgaa ccttcgggtt ggcggagatt tcatcgatga
     5461 ggggttgcgg cgccttaagc tctggcgtgg ggcaaaggaa gtgcacgcgg ttgacaaacc
     5521 gggtgaggaa aagcgcctct tcgatggctt catcgctgtt gcccgccact atcacatcct
     5581 gaccacggaa gaaggccgcg tcacaggtgg cgcagtaaga aacaccgcgc cccagcagtt
     5641 catcttcgcc tttgatgcgg ttgccgcgtc ccattgagcc ggtggcgatg atcactgctt
     5701 taccggtgta ggtgccgttg ttgccgtaaa cacttttctg ctctgcgagc aggttgacgc
     5761 cgatcacttt atctgtgatg aactctgcac cgaacgagcg cgcctgggcg cgcatgtttt
     5821 caagcaactg cgctccgctc acctccgcgt tcaatcccgg gtaattagcg attttgctgg
     5881 tgatccccaa cgcaccggcg gtcaaacctt tatccagcac cagcgtcttt aacccggcgc
     5941 gggctgtgta aattgccgcg gttgccccgg cgggcccgcc gccgatgatg attacttcgt
     6001 attgttgacc ttcatttacc ataaatagtt ccacaattaa aaagtgttgt tatttgcatc
     6061 catccttcag atgcggaaga cgcgaaaaag tgacatgtga atgtgaataa aatctcaaat
     6121 gcaaaaaaaa cccagcagta caaggtcagg aagaagcggt tgaaggtcac cagaccggca
     6181 gccctgttgg cccgctcgtt ctggatgcag cctgactggt agaccaatga gaacgggtga
     6241 gagaattacg tttcaacaca atcaggctgt acagcaggtt gaggtttctt tgcggaagcc
     6301 gaagaaagcg gcgagtgttc taccgccttt ggccgtacca gtcgcaaaat gaggttgcca
     6361 aacttcccga tcagcaaacg catcgcccca tccagaaagg cgggcgttct catttccatc
     6421 agggttttca acttggccat ttttgctgtg ttgtacggcg gatagcgcag caaagggtca
     6481 aagccggttc catgatcgag cactgcacgc gcgttggata attctctgaa accccattca
     6541 ccgtggtatt tccccatacc gctgttgcca accccaccaa atggcaaacc ggtgaccact
     6601 acgtgattga ccgcttcatt cacacaggcg ccgcctgagg tggtgcgcga caggacatgg
     6661 tcaatggttg cctggtcttt tgagaagata tataacgcca ggggtttatc acgttcttgc
     6721 acgaagtgaa tcgccgcgtc catatccttc actggtagca caggcagaat tggaccaaaa
     6781 atctccgcct gcatcaccgg ggcatcaagc tgaacgttct tcaaaatagt gggagcgata
     6841 tagcgcgctt ctcggtccca ttcaccaccc acaaccactt cgccatcgct caggtattga
     6901 agcagctggt cgaattgttt ctgattgata atgcgggaaa aatcggcgct ctcgcggggc
     6961 gaaggtccga aaaagtcata cagggcgctg cgcaactcag ccacgaacgc gtccaggatt
     7021 ccttcatcgg caagaacata gtcaggcgcc acgcaggttt ggccagcgtt gaagaatttt
     7081 ccctgggcga tacgccgcgc ggcgactttg agatcggctg tgcggtcaac cagggcgggg
     7141 cttttaccgc cgagttccag ggtgaccggg gtgagatgct tcgccgccgc tgtcatcaca
     7201 atcctggcag tggctgagct gccggtgaaa aagatcttgt caaaccgctg ttccagaatc
     7261 agctcattgg gggtgtcgtc accaatcaca aaaaaagcgt cggtgtccag gtagagtggc
     7321 aggagattgg ccatggccat gaaggcggcg ctggcggtac gaggaggttt aatgaccgcg
     7381 gcgttaccgg cggcgatcgc gtagaccaac gggccaatgg tcagcagcag ggggtaattc
     7441 cacgctccta tgatcaacat cactcccagc ggttcaaagg tggtgtagct gcgcgctgga
     7501 tggaggaaca ccggcgtggg gacgcttttt ggcttggacc agttgctcag ttcgcgcagc
     7561 gcttcatcaa tttccatttg cagcagcccg atttctgtga ggtaagtttc ataagggctt
     7621 ttacggaggt ctgtggaaag cgcggtttca aattcctggc tgcgttcttt gagcattttg
     7681 ttgagcatgc acaattgccg gcggcgccac gcgagcggct gggttatccc gcttttaaaa
     7741 gtttcgcgca gatggttgat atgcccgttc agtgtttttt cgtccatgat ggttgccagg
     7801 cgctactgca tcaggcggag cgcccatact ccttaatgaa tgattgctgc agcgaggaaa
     7861 tggcagttga ggtatcaccc cttgcagcga tgctaagcca gagggaatta tacccgttcg
     7921 ttggctgcgg ctcacgcttc agtcgtgatt ggctgccctg atccctggcg cgataactca
     7981 cccatatgga ccgaccgccg caggtttgcg cggcagtcgg tttcttatcc tttaattggg
     8041 tgcaaattat tgggccggtg cctgaagcgg gtcaggctgc ctggtttttc tatccggcag
     8101 gaccagggaa agcacattct ttaagccgat ggtgctcacc aggttgatca agaagagcac
     8161 aaacccgatg ctgaccaccg aaccgccaat gaacgccgcc agcgtattgg tgctgaactc
     8221 ccctccggta tagagggtgc ggcggatcat accgtttgat cccgccatgc ccatggcgta
     8281 ggccattgaa accgagccga tgagccataa ccacaggtgc cagtcaacca gtttggggtg
     8341 tttaaactcc agtttggtga gcaggggtac cagcgtgtag atcactgaga acagcatcgt
     8401 tcccacgcca gtcaacagga aggtgtgcgc gtgcagcgcc accacccact gcgtgttatg
     8461 aaagaccatg ttcaaaccga cgttcgcctg tatcagcccg gcgattccgc ccatcacaaa
     8521 accgaacatg gaacccaggg tgaatttcat cgctgggctc atcttcaccg ggcgcgcttt
     8581 ccagatggtc atgagcgaca cgaagatggt cagccccatg gtgaagtact cgccccaggt
     8641 gataaattga ccggagagga ttttcatcca catcggctgc gaggtatctc ccagcaaatg
     8701 gtggctccat acccccattg agaccagcag gtctgccatg atgaccgttt tgaccacacc
     8761 ggcgccaaaa agtttgcgcc cggtgagcag cggcaccagc aggtaccaca ccccggcggt
     8821 gtagatgagc gcgttgccgt cggcgaccat atccaacccc caccaataaa tgttcttgta
     8881 aatgagcgcg tccactgcca gcgggttgag gggatgaccg aagagcgcga acagaccata
     8941 aaccaggatg agtaccccgg ctgagagcag cacgatggcg ttgatgaccg tatcaatgga
     9001 gccacgcgcc accgccacga taaagggggg cagcccgttg taatccaggt tgacggcggt
     9061 tttatcctct ttgccgaaca gtttcagtaa acgcggtata ccgaaagcgg cgcgcaggaa
     9121 ctgccccatg gtgtaagagc cctcaccgtt cttgctcagc accgtcttga atatgttata
     9181 tgaaaagagc aggatgttca ccataatggc tgccgcgccc aacatgaaga agatgctgcc
     9241 gacgaccggt acgcggtcaa acgccactgg cagcggccag taaagtgtgt acatggcgtt
     9301 aaacaagccg aagaatcccg ccgcccagca ggtcatcaca ccgatgatct gcagccagaa
     9361 attggcaacc accagcttag gatgtttgac cggcttttgg atgagatacg gaacgagaaa
     9421 ataaaacgcc ccgaagactg ccatatacac gaagccataa atacccacga tcggatgagc
     9481 ggagagcatg gcgtaataca gttcggcggt ggtaggttcg acatcgccgg gtctgaggtt
     9541 gttgagcagc gcctccatac cgtgcagcga atcaatattg tattgctgag aacgcatcag
     9601 catgccctcg aaacctgaaa gcgccaggaa gaggagagcc accactgcga aacgcagcgt
     9661 ccatttttcc atgttgccaa gagtggtcat ttttcttgct ccttatttca ccacaatcgc
     9721 gtcgcgcacg accatatcgg aatgtttggg cccggagtat tctgtggaac ggacgtcaaa
     9781 ggtgcccgtt tcatcgaact tccaaatgat ctggttctcc ctgccgggca gcacctgcat
     9841 ttgaaagacc atactgttgt cttcgcggaa cacgccgaag ccgtaggtca catccgctga
     9901 ggtcgcggag aattgcacga actcgccagc cttgatctgt accggtgtat ccatggtgat
     9961 ggtgtagtcg ttataggcaa ccttcaccac ctgcgcgggt tcagggtttt gccagagctg
    10021 ccacgactgc cacgggataa gagaagaaag ggtgacgaca ttgacgataa caaacacaat
    10081 gatcaaaacg tacaaccatc ttttttcggc cttttccatt ttatttgcct ttctccttca
    10141 ccaggtgggc aaagataaga aaaaacaaag cggccaacgc gatcagtgca gtatagatcc
    10201 accacaccac ttgagaaaga tgcatcttat cctccttatt gaaaatcagc ggatttcgtg
    10261 taaataatat aacaaaaatg aaacaaaaaa gatatggatt ttgggaaacc tttcatgcat
    10321 acgaacgttc gatttcaccc atccgaaaat tcgggaatgt gttctgcctg aacggtcaat
    10381 gatcaagcct gggattaccc ggtagtaaaa aaagcgctcc tgttgattgg tatattcagc
    10441 caaaagaagt agttttattc tatgtaggga tgttacccaa gaaaagaaag gttattttaa
    10501 ggtatttaac aatccgaaca ctgctcttat taaaagatta gacaggaaaa caaccagtca
    10561 cgtccactgg ctgtttggaa cctgcttgta aaccgtcggc ttgaggttgg ataacccggc
    10621 cggctttggc ttgccgccgc cgcatgagaa cggtgtttca ggtggtggtt tctttccccg
    10681 cgctgatctt gccccgcagc tccgcgacca gccgggcgtg actttcgcgc gtgatggggt
    10741 acttgattaa cagggggatg gtgatggcga acatgagaac gggcaccagg ctgaacagga
    10801 tgcggatacc cagcagggtg aaatcggatt gaggggcatc aggaatatat ttgaacgacg
    10861 ccagcatcca accagccgcg gcgatggcga acgcgttggc taacttggtg atgaagttcc
    10921 acatcccgta gtaaattcct tctcttcgct cgccggtggc gatctcgtca atctcgatca
    10981 catcaggcac catgctccac gggcagaccc actgcgcgga gagccccatt cccgcgacga
    11041 tcgacgcaac atacaccagg ggggttggac ctttcgggaa aaagaagata ctgagaattg
    11101 ccagggaagc gatacccagc cccaaggcat accctttgcc ctttccaatc cggtccacga
    11161 ccattttcat cgggtagagg aacaccccga tggtaaccag gagtgaaatc atcacgaaag
    11221 gcatttgatc ttccatttga acctggtatt tcaggtagta tggcagcagg gtggtcaaga
    11281 gtgtgaaact cacattggca atcaaaaagg cggtcaccag gtgcacaaaa ggtttatttt
    11341 taaatgtgac caggaaggct tttacggcag gcattttcgc gggctgtatt tctgacgctg
    11401 gttttccttt gacgccgaaa gcagtaatta gaaccaccac agcagcgtaa atgccaaagg
    11461 tcaaccccat tgcgctataa ctgccctgaa gagaaaaccc caggttcttt tggtacaggc
    11521 ctgccaccac cgtggtcaga gccgcgccaa ggatataccc gacaacggtg aaaacttcgc
    11581 ggaccgtggt gacggatgtg cgttcatcat aatcagaggt caactcgggg gtgatggcgg
    11641 tgtacgccac cgcgataaag gtatgaactg aatcgaacaa taagaaggtg aacaatacga
    11701 ttagaaaagc cggtattccg gtgagtccgg ctggaattga gaacatcagc caggtggata
    11761 gcgccagggg aatcgccagt aagagaaggt agggtctgcg ccgcccccaa cgagacctgg
    11821 tgcgatcgga aatatagcca aagaggacat cattgatcgc gtcccaggtg agtttaccca
    11881 ccatcagcgc tgtccctacc atggccgggt ccagcagggc gacatccgtg taataaaaaa
    11941 ggagaaagaa ttgaacgatc gacgtcagca tcgcgatacc tgaatccgcg ataccgtata
    12001 aaagcttggt gtgcgtagta actttttctg tcatgttggc ttccttttgg ttcatttgta
    12061 attaagatgg tttatttcga ttgaagggaa tattttttcg ctgccagtaa gaactcgtca
    12121 gcccggacga ctttaacttt gcttgggtcc agggttcgca caaaatcgta aacatcgctt
    12181 actgtggtgc agtaggcaaa aaggtgcacc ccgatgaatc tcggagtact ggatggcgta
    12241 tccaggattt tcttaacgcc tgccatcacc tcatcgcttg aatccttgat ctgcgataga
    12301 tgcggccaaa ggttgcagag aaagaccttg ttatgaccga acttcaccgg ccgatgcttg
    12361 agataaaaat accctgagag ataccctatc aggttgtctg gcgcttgcag gtgctgctga
    12421 atgactttgc taggcgggtc aggataatat gacgtgatga cattgatatc cgccttcgcg
    12481 caaacttcgg ctgagcggcg taaatactcc ggcaggttgt catgcagggg agggatgatg
    12541 taccctgctc cagacggacc ggcaatcagg caatcgttgg gtgtcagtgt gctgtaaaac
    12601 agccccaaca aagccggggc cagctccgcc aggagaggct gcatttccca attgaaagga
    12661 acgagaccgc gctcctttct gcgccacccg cccagttggg cggtgctcat taataacaat
    12721 tggtcaccat ccgagtaggt gaaggtcagg taatctttct caggctcgag cgccacatcg
    12781 gcttcagaaa catggaattg ctttaatggc cccgggtcgg gcaacgcgct atggaaagag
    12841 aaattcgccg ccaggtgcga gggaacgagc cgcagaccga tggcgctcag caacgccaga
    12901 aaagcgaatt cgtcatcacg attggtgtgc cagccaaaaa tgatgccctc cgggtgccgg
    12961 gcaacttttc gtaatagcga gatcagcaga cccgtttcgg ggtcggaaga cattttctgt
    13021 aaggcgaaat tcttcaccca gcgaaaaagg ccaacgttat acaacgcgtt ccgcagccag
    13081 gttggacctc ccaggcaaag ggtgagcagc ttccagccga agttgaaagc tcggccactg
    13141 cctttgctgg ataggttaca ggtgaagatt ttctctttca ccacataatc caatagctcc
    13201 gggcggtgcc agtgtggttc aatgttggcc acccgatcgg gtcggcagcg ggcaaagagc
    13261 tgctcaagcg cccaggcgtg gagcgcgacg tggtcttgcc aacagccgcg caggtctttt
    13321 tcaattttga gcccaaactg cttcgaccat tccacaaggt caggagcgat ggggagccag
    13381 ttctccacac tgcagaacat caccgccagg ttcacagtat cctcctgctg ggggtcccaa
    13441 acgacaaacc cacggcagag gtcctggaac tgatgaacgg cgctggagag atcattcagt
    13501 ttcgtaacat gaatggggta aagtttctgg taagcgttca ggttgtgctc atcctgcctg
    13561 cccatcgcat cccgaaattt gcttacccac aggtcttcgc ccagtgcatc ctggttggtc
    13621 tttctggatt cggggtcatc gtagatacca taatcaagga acaggcgcgg ctggtcacgg
    13681 ttggctaatc cttgcaggca ggcggcagtc acctgctcgg cgtagctcat gtggctgaca
    13741 tctaaaacga atatcttgat tgtctcggtc atggcagtgg tcagtatccc cagtattcgc
    13801 gcgtgacttt gctccacgct tcaataccgg aggggttgac gggatatgcc cggtaatgcg
    13861 cgctaacctg gccagctttc tgatacaggg cgttcattaa ccgcagactg ttttctgaaa
    13921 ataacccttt cttcatgcca tctgataatt gctcacaaat ggcggcccat ctttctgccg
    13981 ggaggttcag ttcataattg agattcatgt actcgtagtc atcctggcaa aagatgatgt
    14041 tatgccgatt aagataatcg atctcgtggt tgggcagccc gttcaccggg aacaggaaca
    14101 tcatcacgcc ggcgaatttt gcgccctgac cgtggaagta gcgaacattg tattcccaaa
    14161 gcccttcacg gctaacgtca ccatttttta acgctttcac catcacttcg ctggcgattt
    14221 tacacgcggt gaacccgctg gtgataccct ctccggagaa cgccttgttc tggtaaacac
    14281 agtcgccgac gccaacaaag ccattcccta cataagaatt gggggaacgg cggtacggag
    14341 agcagccagc gcgtttggct accagcttgc ctggttcgcc aaaagtcgcc tgccgccatt
    14401 gggttaaggc cgtccacgcg tttttcacgc tggcgggggc gataattccc aacactgcac
    14461 catcaccata agacttgttc cagtagcctg gggagccaag gtaggaatta ttgtctacag
    14521 ggtaaccggc gggtatttcg tcacgcagtt ccaatgtgca ataaaaagtc tgctccgcgg
    14581 gcaccgggca attctccacc ccgaaatcat ctgggagctt ggtgcgcacg gcgctgatga
    14641 gacctgaagc atcgacaacg attctggcgc gggcttcaaa cggctgttga ttgaaagtcc
    14701 cccgcacacc gcggcaatac ccgtcttcca tgatcacctc ttttacctgg gctccgccta
    14761 cgacccgtcc gccagattcg cgcgcgtagc catgcagccg ctgcacaaaa gcaggcatat
    14821 tgaccacgta gaacgggtag cgcacttcaa aatacccgct cttatccacc gcccaaactt
    14881 tgttggctgg taccaggtgc attctttcag gaggcagcgg gtgagggatt tcgaactcat
    14941 cgaaacgcac ctggtcgaga tgaatgacct cgatatgcgc gccaaccttt tcgagaggca
    15001 gcgtttctag aatgagaaca tcgcttcctg cctgcgccag tttccacccc aggtaagacc
    15061 ctgtaggtcc tccccccaca attaccacat ccgcgttcca gtgctcagac attgtgaacc
    15121 tccatgcgtt gatatagagt tgatcaattg aggtgttata tctggagatg agtataagag
    15181 aaaccgggaa atagcacaac catcaaacga tggaaataaa aaaacctgcc gcgaggggca
    15241 ggtttttgtg attccaggca cgaccctact ggctgaattg agccatgatc aggtcattgg
    15301 catcgaacag gaacaagcgg ttgccttcga cgctgaaatg cgtcactttg gccaggctgc
    15361 tcaggtacgc agcttcttgt tccatcacac cttcctcagg acaagccatc attgttaccg
    15421 caaacgtgtc gaaggacagt ttctgctcct gctgatggaa caacccatgg taactattgc
    15481 agccgcttga acctgagagc gcaccatctt tgaactcggc tgttatttgt gtatctggca
    15541 ccaaactcac caggctctgt ccgtcgttga aagaggtcag gttccaggtc ttcccttcca
    15601 ggctcatgtc ttgcgcagca aaggtcagca caaccgcttt cgcgctatcc atgatcagta
    15661 atttgccttt ctcaatccgg tattccacgg cggattccag ggctttgagg taagcggctt
    15721 cctgctccat caacccggcg ggctcaaggc aggccatcat ggtagtggcc agcgggccaa
    15781 tggtcagggc tccatcttta cctgtatacg tgccgttgaa attgttgcat gaggcattac
    15841 cgcccacttc ttggtcacca cgaaaagaca gggtcgggat tacattctca atcacgggct
    15901 tgccgttgat ctgttctaaa acccagtgag taccggtcaa agaaggcgtg acagcgctcc
    15961 catttccacc gcaggcggat aaaaccaacg caagcaccag taaagaaaac aagattctat
    16021 ttttcatcta actactcctt atgttgtttt gttgtgaata agacgatttt ggtaaggaat
    16081 tagttcccgg ccgatcgcct tagtgacccc attgtttttc ctctgcaggc tggttcaaga
    16141 aatggatgag aagaaattcc tccttttaaa aaaactcaat ctaaattgcg gataagtcca
    16201 tcgcgcaaac acagaaaaca tcaacattga cctttgaagt gagcggtaac ctgtgcttct
    16261 acttctttca gcacctgtag gaccggcacc tggttttgtt cagccagttg aacacatcta
    16321 tcaaactccg gagcagcctg caggatatca tccccactaa ccttgagctt caccggcact
    16381 atcccgtaat gggtttcaac attgcgaaat tcgcgtcctg actcgtgccg ccagatgggc
    16441 ttaaccctga cgcctaatgt gctggtttct cggaggataa ggtcgctgat gttctgctca
    16501 tcttctttac gcgcgatgac tgagattttt gtcgcagggc ggtttttttt cataaagatg
    16561 ggcgtgaaaa aaacgtccag cgccccagcc tggaacagtt tcgtgatcac gtgcccgtaa
    16621 acctgcgggt tcatgtcgtc gatattcgtt tcaatttcca ggtgggtgca gaaatcatct
    16681 cctgcttcgc cgatgaaaac tcgtaagatg tttggccact ccaaatctct ttgccctgcg
    16741 ccaattccca cacgttgaat tttcatttca ggctgacgga aaacagccag agccgaaagg
    16801 atagccgcgc ctgtaggagt gaccagttcg cttgtggctt tagaacccgt gaccggcgcg
    16861 ttagccatac gaatcaactc cattgtggct ggcgcgggca gcgggagcag cccatgctgg
    16921 gtattgacct gcccgcttcc cagcggtagt gcggaagaaa aaacctgttg aacaccgaaa
    16981 tattccagcc ctattgccgc gccgacgata tccaaaatgg aatcaaccgc gccgacttca
    17041 tggaaatgaa cctcttcgat ggtcgagcca tgaaccctgg cttcggcttc agccagcttg
    17101 ccaaagattt ttttactggt ttgctgcacc tgttctgaca acccgctgga ttcgatcaat
    17161 tgacagatgt cgcgaaaatg ccgatggtgc gtgtgaggat ggtgttggtc gtcttcagcg
    17221 tgttgatgga tactcaattt taaaagcgtt gcctgcaaag aacccttgaa cactttcgtt
    17281 ttacccgctt cttgaaactc agggaggtta agctttttca actgcgtaga tagagtctca
    17341 accggtaaac ccgcgtcgag caacgcgccc aggaacatat ccccgctgat tccagaaaaa
    17401 caatcgcaat ataaacaggt catagagaca atccttcgtc catgctgcca gatcggtatc
    17461 cctgcagatc taaagcaaca tgatggaaac cgatttcttt aaaatagctt accacatcct
    17521 ctcgcatctc caacagcttc gccaggtggt caggttctac ttcaatactc gcgtaatgct
    17581 gaagatggcg gacccgaaca acgggaaaac cgcgttgatg caagtaattt tcagcgctgg
    17641 caatttgatc aagcctctcc tccgtgatgg tcatgccgta ggggattcgg gtagccaggc
    17701 agggagaact tggctggttc catatgggta gccctaacgc cctggcaagc cagcggattt
    17761 ccgctttggt gagcgcgttc tgcgccaggg ggctgaatgt gcctgtttct ttgacggcct
    17821 ggcgtcccgg tctgtattca tgctggtcgt cggcgttttg accttcaagt agcacggcgt
    17881 aatggttatc ccgggcgtac tcccacaaaa tattgaggat gccgagttta caaaggtaac
    17941 agcgttcagg agggttgttt tggaattttg cattttgcag tgggtcatgc gtgaggaaat
    18001 catgtttgat cccatactgt tctacaaacc ggaacacatc ttgttgaaga ccagcgggtt
    18061 cgagcggtga aaaaacggta accgcgatca tcggaccgcc catggcttgg gcggctgcat
    18121 acgccaggaa actgctatcc acgccgcctg aataagcgat aaccgctgac ggataagagc
    18181 tcaattcagt tatcagagac tgccatttgg ctttgagggg gtgtgtaatt ccggcgtttt
    18241 ccaggcgctg gttggcttgt tcaagagggt tcatttccgc tctcgttgct ggataaacgg
    18301 ttgattttgg cagccatgca gccggcgcca aaaccgttat cgatattaac caccgccaca
    18361 ccgctggcac agctgttcaa cattgccagc aacgcggcta atccttgaaa gcttgcgcca
    18421 taaccaacgc tggtgggaac cgcgatcagc ggagcggaaa ccaacccggc gaccacgctg
    18481 ggaagcgcgc cttccatccc ggcgacggtg atcaccaccc gggctttctg caagacaggt
    18541 aaatgactga tcaaacggtg aagccctgcc acgccgacat cataaatgcg ttcgacctta
    18601 tttcccatca gctctgcggt gatggcggct tcttcggcaa cggggatatc tgatgtgccg
    18661 gcagtaagca ccacaatgcc ggggcgcttc acctgcctgg tatcagggct ggtgaacaat
    18721 aggcgcgccg ttgaatcata ggttacaccg ctcagatgat cttgaatttc agcatataaa
    18781 ccagggctta ctcgtgtcgc cagcacctgg tggtgactct tttgtaaccg tgtaaatata
    18841 tccaacacct gttgcggcgt tttaccctgg gcgaagatca cctcaggtaa ccccgttcgc
    18901 aaggggcggt gatggtccag ttttgcataa tcgttcagcc cttcgtaggg aagcgttttc
    18961 aactgatcaa ttacctgggg cacagtgagc tctcctttga tcagattctc caggagtttg
    19021 cgcagcgttt cttgatccat tcaacctctt atccggtcgt tcttcttgcc tgagatattc
    19081 gcataggatg gtagatcttc gaccgattga aatgattata tcaacccatt ggtcatcagc
    19141 aaccggtcat ttctgaggat gtccaggctt ggcccgtcag cggcgatttt tccatgattg
    19201 aggagaacgg ttctgggggt gagttgctcg accatgttca tatcatgtgt ggcgatgatc
    19261 atggtttgct gaagttccaa cagcaggtca atcaattcgc gcctggcgcg cgggtccaac
    19321 ccggcggtgg gttcgtcgaa cgccaggatc tggggctgca tcgatagaac ggtggcgatt
    19381 gcgattctct tcttctcacc gctgctcagg tgataggggt tacgcagcgt atagtcttcc
    19441 atgtgaaccg ctttcaacgc cagcgcgact ttttcccgaa cgatgtgcgg gtcaaagccc
    19501 tggtaggttg gcccgtaagc gacgtcttcg taaacggttg gagagaaaag ctgatcatcc
    19561 gggttttgaa aaaccagacc aaccattgcc ctgaccctgg gcaggttgtt tttattcaac
    19621 tcaagttggt ttacgcgaat catcccgctg cccgtaaaaa tgccattcag gtgatacaag
    19681 agggttgatt taccagcccc attggctcct acaagggcga ccttctcacc cggctgcacc
    19741 aacaaattga tccccgcgag ggcctgtttt ccatcaggat aagaaaagga gagatttctg
    19801 atttcgatga ctggttccaa tcctcaacct ccggtgatta cacccaacaa ccataatatg
    19861 accagaagaa taacccctgc agcgagtaca agcaggtctt ttttctgtaa ccgcgttgtt
    19921 ttctgagcag caggttgacc tgtataccct ctggataaca tcgcggcgta tacccggtca
    19981 ctgcgttcaa tcgagcgcaa gaacaaattc ccagccatgc ccccggtcac gcgcgctcgc
    20041 caaaacagag accctcctga gcggttcgac cctggtgctg ttgcgctgcg gctggttcgc
    20101 gctctcaaca tgcgggtgac ctcgtcactg atgacaaata gatagcgcca catcaacccg
    20161 atgatggtca aaaaaagcgc gggaaccttg agctgggaga acgctgtaag taggtcagga
    20221 aagcgtgtgg ttgcggcgag cagtaccgcg gccattaccg aaacccacgc tttgatggca
    20281 atactggcga agcggtaaaa ccctaccacg ctataagtta aggaaggggt gttcagtaca
    20341 ggaatttgac ccagcggggc aggaccagta aagaccagcg gtaaggccgc gagcacaaaa
    20401 ggtaacgcga gcaaagacct tttcagcacg aaggcaatcc ccaggcggga agacagagat
    20461 agagaaaaca tccaggtgaa aaagaggata tacgctggcc acgcgccgga aggggtcaga
    20521 tttaaaaaaa cgataaacgc aagggtgaag atgaccttca cccttgcatc gattcgatgt
    20581 acagcactgt ggtttggtgt gtaaagttct cgcaaccgtc tacctttggg cgtcgtcttg
    20641 agcggatggg cgcttttcag cgcgggcgat gccataagca acaccaaaga cgatcaccgc
    20701 ccccagtata ccagccacaa tggttgccag cgccgggttt gaaattccag gcatgctgta
    20761 atcggggacg atattataaa aagcttccct ggcggcttca ataaaaccat gtttttccgc
    20821 gacccattcc aggccatctg ggtggctgga cgccagcgga gaaagcacaa ccagcaccaa
    20881 tgatagaagc aaacccccga ccacgacacc aatattgcga gcgggcttgt tcgcgtcggc
    20941 tttaagcaga tctttacggg cagcgaagat gaacgccagc gctccaatgg tgatcaatcc
    21001 ctcaccaatc ccgatgagcg cgtggatggc gcccatcgcg ggaacagcga tattcgcggg
    21061 agaggtgccc gatagcgcca gttgcagcgc gcaggcaagc gaagcgataa aaatggacga
    21121 ccacgcggct acgaaaccag caacgaacag cccccaggtt tgttttttga agagcttcat
    21181 cagcaaggtg aagagcgcgt aagccacgaa gggaccgatg atggccatgt taaagaggtt
    21241 ggcacccagg gccaaaatgc cgccatcctg aaaaagcaag gcttgaacgg caacgacgga
    21301 agtcattacc agaacggcag gccagggacc taaaagaatg gcggcgatag ccgcgcctaa
    21361 taaatggcct gaagtaccgc cggtgacact gaagttcagc atctgaccgg caaaaatcgc
    21421 tgcagccaat acgcccatca acggaacctg gcgttcgccg atagtttggc cggtcttgcg
    21481 cagggcaatc ccaatcaaga ccacagaaat gacccataag acaattgaaa gcaggacaga
    21541 gaggaatcca tctggaatat gcatggggtt tatcggtgaa aacaacattg tgagcctccg
    21601 gtgaaagaat gttagaatgg agcgtaaggt aatttcagga aaattataaa tcaataattt
    21661 gcatatgcaa agatttgcat tatacgtttt ctgtcggagg gacaatgcgc gcctcttcag
    21721 tagaacaagc cattctggaa actttatcga aagaacattc tcacctgacc tctctacagg
    21781 tatttgaaga aatccgcggg cgcctgccag cggtaaatca atctactgtg taccgtgctt
    21841 tagaacggct ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcgatg
    21961 cgcagcaaag cctggctgaa tccagcttcc aaaaacccta tgacgcgttg accctgcctg
    22021 agcagcaggc ggtgcagtta caggtaaccg acctgctgaa agagaaccgc tatgaccctg
    22081 aaagcggcgt attaattctt accgcggcgg aagcggcttc tttccaaaga cagatacagg
    22141 tatggactga ttactttgct tcaaccacct cgagctctgg gctgaagcaa gacctgatct
    22201 cccacccgga agaaataaaa caactgaccg ccttcttcgc ctggtctgcc tgggcgtcgg
    22261 tggcgcaacg gcctgacaaa ccctactcgt ataccaataa cttcccttat gaccccagcg
    22321 tgggcaatac cgccactgct gacacggtac tttggagcgc gttgagtttg atcgccctgc
    22381 ttggcggatt gggcctggtg ctgttcgtcg tcgggcgctg gaacttcctg ggttggcggg
    22441 gaaagcaaga aggtgaactg gaagagccga agccgattgc ctcagaaatc gccacgcctg
    22501 cccagcgcgc cacgatcaag tattttgcgg tcgtcgtgct tctattactg ttacaggtgg
    22561 ctatgggggc tctcgtagcg cactacaggg cagacccttc tactttttat ggccttgatc
    22621 tttccaagat cctgcccagc aaccttgacc gttcttggca tttacagatc gccgttttct
    22681 ggattgccac ttcgtatgtg gctggcgctc tatttttagc gccgattttg agcaagaaag
    22741 atccaaaaca ccaggtcgcg ggcatcaaca ttctttttgg tgcccttgtg ctggtggtgg
    22801 ctggcagttt gatcggcgaa tggctgggta tcaataacaa gatgggaaac ctctggttct
    22861 ggttgggaca tcaaggctgg gaatacctgg aactgggccg tatctggcaa attctacttg
    22921 ccattggttt gctgctgtgg cttttcttgc tggtccgcgc gcttaagaac gccagccaaa
    22981 acccagattt caagcagatg gcttcactgt tcatcattac ggcgataggc atcccgattt
    23041 tttatgtgcc ggccatgttc atcaatagca tgaccaacta cgccgtggtt gaaacctggc
    23101 ggttttggat catccacctg tgggtggaag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23221 nnnnnnnnnn natctcccag gggaaatggg aaattgtcaa acgccacaag tggactttct
    23281 acttcttgat ggctgttggc ttctggaact tcctgggcgc gggagtgttc ggcttcctca
    23341 tcaacttacc gatcgtcagt tactatgaag ttggcacaac gctcacgcca aatcacgggc
    23401 acacctcttt tatgggcgtg tttggcatgt tgagcctggc gttgatcgtt tttggcctac
    23461 gccaggtgct ggatgatcaa aagtggaaga aagtcgataa gttcgtcgga gtgggatttt
    23521 ggggcctgaa tatcggtttg ttactgatgt cgcttctctc gttgttcccc ggtggggtgc
    23581 ttcaactgct ggacgtgctt aagaacggct actggcatgc ccgcagccag gcgtacatgg
    23641 gtcaaccgct ggtaaaaacg attgagtgga tcagaatggg accggacctg atctttgtgt
    23701 tggccgggtg cattccgctg gcgatcgctg ttctgttcac cctctggcag gtgataaagc
    23761 acaagaagtc cgctgatgcc aaccactaac ttcgagtgaa ctcacctggc agcacctgga
    23821 acagggacag aataatttcc agtacaggtg gtgccaattc atcagaccga gggcatttga
    23881 ccctcggttt ttttttgaat acaatgaaag aacctcatga atgaagcagc gcgcgggttt
    23941 ctcacagatt tgttcaagat gcataaacgg caggtgaggc catgaccagg ttgaactcaa
    24001 cggggcaacc aacctgactg agccggtttt cattattgaa gcaggtatgg gagttcttta
    24061 ggcggtttac cttcctttga gcaacagaaa ccaaggggaa ttatttgcgt ttctcgcaag
    24121 aggggaagag ttgcacgcgt gccctggaaa ggagaacaac tcgtacttga cttttgtata
    24181 ctgaaaagta taatatatac cgttcagtat agaatgggtt gaaaaatggc aaataaacca
    24241 aaaccagaac acgagaatgc caaccgaatc ctcgccacgg cctggcggct ctttcaacag
    24301 aagggttaca aaggagttac catcgatgaa atctgctctg gctgcgggct gaccaagccg
    24361 accctctact attattatcg agataaagaa ggcctgtttg tctccgtctt gcagcaccaa
    24421 cttgccttat tcaatcgtgt attgacgcag ccaggcaata ttgaacagca gctgcagcgc
    24481 ctggcggtgg ccattttgga gaacttccag attgaataca gcgtattgat gcgcgatcgg
    24541 gctcatatta aaaaaccaga aaaccaggcg gcggtgcgag aagctttccg ctctggtctc
    24601 ttcaaccctt tgttattgtt aatggagacc ggcctgaaaa atggagactt gcaagcccgt
    24661 aaccctgaag tgttaacgct tgcctttttg ggcataatca ataactttat taaccgcggt
    24721 gcagaatcag gtaccagcac agcggagttg gcggctgaat taacgcagtt gtttttacaa
    24781 ggagcaaaag ggcagagggc aggttccacg tgattgaagg tcagatgcgt ggtcttgaaa
    24841 gcacggtgac tttttttgat ttgctgtgcg catgttaatc gaatattggt tcatgaacga
    24901 taaaaacaac ctaatcaggc aaggagaacg ttagtgtttg aacaacaacg atcgatagaa
    24961 gatagaacaa aaaacagttg gtggtcatac ctgaaaccta ctttaccgaa aaggtggctg
    25021 ctcatcgtgg caggcgtaat gtggagcgct gtgggaatca tgttactgaa ttacgccgtt
    25081 acctggttaa ccctctcttt ttcaattcct gccattctgc tgggcnnnnn nnnnnnnnnn
    25141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25201 nnnnnnnnnn nnnnnnnnnn nnnnntcagc caggtagcgc ttctggaact catcccattt
    25261 cagcgggtca tgcccaaacc agcgccgcaa atcggtactg ggggcgacgt ctttcaccca
    25321 ggaatcaatt ttcagctctt ctttcttcat cccacgcggc cacaggcgtt ccactagaaa
    25381 acgaaccccg tcactttcct gaggtctttc ataaactcgt ttaatcttca ccatagttgc
    25441 tcctttccat taaggctgac ctctttgcac tgcagacaga cgcgcagcgt ttgaacatca
    25501 cccacagcgg tggcgtctac ccagcgcgct tacccattac gttttccatt gtaattcaaa
    25561 tctccgggca aatgctgcca acacttagag ggcaaatccc tatcagggcg ccggcactga
    25621 gtccatgctg ctggtaaagg attattctct caggtaaagg tgttttttga atacctctgg
    25681 gtcggaaagg accaaatatt caagcgtttg ggcttttatc gtttcccgcc agtgcgcggg
    25741 tggaaagaat acccttttaa tcctttcgcg ctctattcgc tgccgggaaa atcggtgatc
    25801 acaccatcgt tgagcagaac cccatttact ttatagacaa ttttcccagt gcagactgta
    25861 gtggaagaac tttcaccttc aatcgttacc gttcccccgc caattgtcca gttaacgtct
    25921 gcggtggtta ttcctgaagc gatatatttg ggagtaagtt catttacttg agtacttgca
    25981 ctttcgccaa agactttaac ggtattttca gggctggctg gcagaagctt gtcgaccact
    26041 gtcgatgtgg tgagatcgcc atacattaag aagaatgcgg agcatccact gataagaagc
    26101 atggcagaga tgatcgatat caggaatacg cgtttgtttt ttctaaatag caatgggaaa
    26161 acgaaaaagc acgtagatgc catggcaaca gggattgctt tgttgcctgg taacctgctc
    26221 tcgggttgtg ggatggaaac aaaggtttga acagtgatcc cttttgaatc taacaatgcc
    26281 ataccgttta ctacaactgc ggtgtcacct ccaaatgtta ggtcaccgcc ggggctgagc
    26341 gtccaggtgt atgtgtttgc tccagcatct tttacatact ctccggggat ggctggtata
    26401 taagaattga tgtatgaggt cagttgcgcc atttcttccg gtgttgtggg gtcattatta
    26461 tcgttagatt cattaccgct tgagtcacaa accatgttgt tggatacgat gctatcgaat
    26521 acaaatatcc aatcaccact aatatcgaga gggcgaattt tcgcggtaaa ggttttcgca
    26581 ccatcggtag ccactgcaac gatgcgaatg ccggataaac ctgttttcga gtgtagcggt
    26641 ccgatcaccg ttttggctgt tccatcattg tattggatct ctccaccctc ttctgtatag
    26701 taaaaaggac atccaggtga gacttcaatt tcaaccggat atcccgcaag ctcggcatta
    26761 cgcggcgttt caagcatcaa cggataaacc ccacctttct ctatgactgc ctggatatcc
    26821 agttcagttc ctgtcccgcc gctggtttgc ttcatctggg gttcataacc cgttttctta
    26881 aaattcaaat cgcctttgtt gtctacaggg gtgactgatt cgccgtaccc tgtcccattt
    26941 ttcacagaat tcaagagata gatcattgaa ttcgcctttt gaggggattt tcccaacagg
    27001 taccgcgcga aatcatcgag gttggcactg atcttttcca ctgctgagga atctattggg
    27061 aatgtcccat tattgatcgc gttaacgaat tgctgcggac taatagggca tgctgatgga
    27121 ttgtcacagg taaatacttt caataattgt gcatggaggt aaagatcatc attccattga
    27181 ttgggcgcga attgaaaagg ggtgcgctta tcagccccaa ggctcgtggt cccgtaacta
    27241 agcaaatttt gatcgatata ttttggctcg taaagcatca ccatgtattc agccgatgtt
    27301 tcaagccacc attttgaagt gctcgaaata cccagcttat tactccaata
//
LOCUS       DAOSPM010000013        26924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1796_length_26924_cov_14.109941, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000013 DAOSPM010000000
VERSION     DAOSPM010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 26924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26924
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1796_length_26924_cov_14.109941"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLW25_02345"
     CDS             <1..557
                     /locus_tag="PLW25_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOS53359.1"
                     /translation="VFQKHLYFRKVYLYLLYISAIFRSKLIKIDAIDKKISDLLLQDG
                     RMSCAQIAKRIGGEVSERAVRYRINRLVENGIISVRGNVNAENIGLVVCADVFIEVEP
                     SQIKTIAMKLAEYESISYIAYATGDTDISIQLFTPDNSTMFDFVTEVIGKIPGVRKTH
                     ISFVPIKIKDDHVWPIPSFIVTQD"
     gene            659..2260
                     /locus_tag="PLW25_02350"
     CDS             659..2260
                     /locus_tag="PLW25_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOS53360.1"
                     /translation="MAPEIDKATLVRSEKQKLKRELTILPLFALIYFTVCGGAFGAEP
                     MVGLSGPGLALLLMVITPLVFSIPNMLMVREMQSMMPVEGGYYHWVKQAFGPFIGFMS
                     GWMNWVVSWVDVSIYPVWTAWYLSYFIPALSDGATIGGIHFSSDLLSWLVSAALIITI
                     SLLNVRGAKLSGLTASWLGVAMIIPIIIMSGFGIYAWIVNGTPSNIPFLPGGAEVSGR
                     NLLGALSVGIYVAMWNFMGWELPTAAGDEIKNPKKTYPRAMVLVLIATILSYSVPTVA
                     GLYGGAGEDGQYLAWGLEESAPGTGIGPILNDYGIADEKMLTWGIDPGSDAGWEFPTI
                     AHRIGDKVAGKDSPLSYFLGAIVGISAIFSMVGLFIGNGLGGTRVPYAMAEDGMMPTW
                     LNKVHPKYGTPWVSILICGAFYLIFSTSAFQNLVVIDVFLNMLVLMAEIFALVVLRYK
                     KPDIPRKRVPGGIWGLIYIVVAPLTIIIIAIVSQIIDYGWQGAVGLALIAMAIGAVLY
                     FPIRKWVKKDIPDVDPYVLEAAEEA"
     gene            2286..3491
                     /locus_tag="PLW25_02355"
     CDS             2286..3491
                     /locus_tag="PLW25_02355"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOS53361.1"
                     /translation="MKVLLVGVGGVGEAIAIMAAKRPWLEKMVLADFNKERALEVQAK
                     IKDNERFPVEFVDARNRGMIEELAKKHKVDLIMNACDPSFNETIFDAAYNVGTNYMDM
                     AMTLSHPHPETPYTKTGIKLGDYQFARADKWKEKGILALLGLGVEPGMADVFAKYAAT
                     HLFDEIDEVNVRDGANLIVKGYDFAPNFSIWTTIEECLNPPVIWEKGKGWFTTEPFSE
                     KEVFDFPEGIGPNECVNVEHEEVLLVPRYIDCKRVTFKYGLGDEFIGILQTLHLLGLD
                     NKEPIKVGKVEVAPRDVVAACLPDPAHLGPLMEGQTCAGTWVKGKKDGKERNVYIYQV
                     ADNKESMDKFDCQVVVTQTAFNAVIGMELLAEGIWKATGVEGPEFFDPLPFMDRMAGM
                     GFPWGMKEW"
     gene            3710..4981
                     /locus_tag="PLW25_02360"
     CDS             3710..4981
                     /locus_tag="PLW25_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS53362.1"
                     /translation="MVNYQENLKHLSPVWTHLTDIVVDHAEGCYLYATDGRKMLDFTC
                     GIGVTNTGHCHPKVVEAIRQQAGLLIHGQVNLVVHKPLLELVDELRTVLPSTLDGFFF
                     SNSGAEAVEGALKLARHATGRPNVIVVQGSFHGRTNATMAMTTSKNSYKIGYQPLMPG
                     VYVTPFPYALRYGWDEEKASQWCLDELELLLMTQLAPQETAAIFIEPIIGEGGYLVPP
                     KTFMKGLRELTQKHGILLVADEVQSGFGRTGKWFGYQHFEIEPDIMTVAKGIASGMPI
                     SGVISRLELMSKWIPGSHGGTYGGNAVAAAAAVATIRTIKEEKIIENVVARGAQLKAG
                     LAHLQEKYPQIADVRGLGLMVGTEFRDAHGKPDKKTTKAVQAECLSRQMMLLTAGPWD
                     NTIRWIPPLVVTESQMNEALNIFESALAAVI"
     gene            5018..6403
                     /locus_tag="PLW25_02365"
     CDS             5018..6403
                     /locus_tag="PLW25_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOS53363.1"
                     /translation="MSELIDHLLAKAREAQKVIEFWPQEKVDEMILAVGWEAYKRETA
                     ETVARLAVEETGLGVYEDKVLKHQKKTIGVLRDLQGEKTVGVIERVPEKGLVKIAKPM
                     GVIGAITPMTNSSSTMPCNGLMILKARNAVIFAPHPRAKKTCAVTCEEMRKGLAKVGA
                     PLDLVQYIEEPSIELTQELMSKVDLVLATGGAGVVKPAYSSGKPAYGVGAGNATVVVD
                     ETADLPVTAAKICKGKIFDNATSCSAENNIVIQESIFDDLLEELKKTGGHLVTGEDRA
                     KLKAAMWPDGVHLSGKIVCKSVKFIAEEAGIHVPDGTKFIMVLGEHIGPEDMFSAEKL
                     SPVLTLWKYKTFDQAVQMVIDITGFSGYGHSCGIHSVNEEHILELATKAKVSRMMVRQ
                     AQSVGNSGDWENGMPFSMSLGCGTWGGNITSENITVKHFLNVTWVSYPIPAEIPDPEV
                     IFAPHFAKYGK"
     gene            complement(6448..6765)
                     /locus_tag="PLW25_02370"
     CDS             complement(6448..6765)
                     /locus_tag="PLW25_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53364.1"
                     /translation="MDKETQMLTIFFRHDQSKSLDEIQAHLRANGFWRDFPPEGMEIV
                     SWVVMMGIGHVVTLRLPQSRLRELNMIVEKKAWKAFTTEFYPTYDFLPHYQQFHAEAA
                     ARD"
     gene            7001..7726
                     /locus_tag="PLW25_02375"
     CDS             7001..7726
                     /locus_tag="PLW25_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53365.1"
                     /translation="MKIEFPTPVSTEEMKIDLREIEGILQGAPMDMPPIPEATVTLKD
                     GRIVYIRGIKDSDIDPMLGYLEKVMKVDKDFYDIVGVRVYGEVLALRRKRLKDPFTMV
                     GLVDGEWLGFANGRAWDKDIAISLHTLTFSRKARLGWPMYYAKTYYAMELLKVKEWWS
                     TFESYNGWRMAGLSMAQPTKPWPEYQHELGGAKVFYLNQDMWHSRVKDYVREIVGQDM
                     DFNVTDEVRKANENFRVPETVDL"
     gene            8029..9765
                     /gene="fdrA"
                     /locus_tag="PLW25_02380"
     CDS             8029..9765
                     /gene="fdrA"
                     /locus_tag="PLW25_02380"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HOS53366.1"
                     /translation="MATFKTEVRSGVYYDSAKLMSLQRSLAGLPGVLDAGVVMGTDSN
                     KELLAHIGLDTPEVKATKPDDLAIVIKAENEKAALDALAQVDGLLAARKGGSEQEYQP
                     RSIESAADMLPDAEWVIVSVAGRYATAVTREALRLRKHVHLFSDNVSVENEIELKKEA
                     RDLGLLVMGPDCGTAMVDGYGLAFANKIRKGPIGIVAAAGTGLQQVATRIHQLNSGIT
                     FGIGTGGRDLKAEVGAITFLMALDVLARDDDTKVIVLTSKPPSPKVAEEVLKVARKAG
                     KPVVVNFIARPITTTRDGNLFFATGLDDAARLAVELAHNPPKLVSDADLPIKKFTAKQ
                     KYLRGLFSGGTLAYEAQHILYGYIPRVLANSPINKEFQLPDAFVSQENCIVDLGEDEF
                     TQGRLHPMMDNELRIRRLYEEAKDPEVAVILLDVVIGYGSHPDPASEIAPAIAKCIAD
                     AKKDGRHLEVVCVVCGTDEDPQNLNNQMAQLKAAGALVDTSNEVICRYVGRILRALAE
                     RDEPKAHALGKPVDLEKIKKPLAAINVGLESFAANLKDQGAPYVQVDWKPPAGGNEKL
                     MNILQKMKSPRK"
     gene            9801..11048
                     /locus_tag="PLW25_02385"
     CDS             9801..11048
                     /locus_tag="PLW25_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOS53367.1"
                     /translation="MVDIEKANALAVEKFMEARPVAITLAKALEIVPGMHENMLLHAG
                     PPVTFERMSGPTKGAMIGALLFEGKAKNPEEAEKLLKSGKIEFSPCHEHMCAAPMAGV
                     ISPSQLVYVIENQTHGIKCYSNLNEGRGKVLRMGAYSEDVLEKLRWMEAVLGPTIKTA
                     LEAMGGIDVRAILAKALHSGDDGHNRLDTASLLWTTQLAPYIAKTAKNTETAFEVTKF
                     LGENALSILNPVMAGCKTMTAAGHNIEGSTIVTIMSRNGTDWGIKVSGLGDKWFTAPS
                     PVVKALYFPGFTEKDACPDIGDSVITETAGIGGFAMATAPALVTFVGGEPRDAINTTL
                     DMYEITEGEHKQFTIPFLDFRGTPTGVDIRKVVEKQITPRVNTGVAHKDPGVGQVGAG
                     VVSAPMKCFEDALIAFADKYGYN"
     gene            11077..12744
                     /locus_tag="PLW25_02390"
     CDS             11077..12744
                     /locus_tag="PLW25_02390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOS53368.1"
                     /translation="MNREDFIKMLTDAELYDLTQDCSIFTPPHPHEKGLEVHFFKRVT
                     GAYGGGQGANGQILNWSNTVGTHLVGERAFNSGGRAIADIPLTDLCGPGVVADISDLV
                     SDYSIYTPEMITSKVEVKKGDILIVNTGYHKYSWDQPAVRNKTAQGGVESMEFGFLVR
                     HPGPSMDFYKWAMDMELKVIGVDCGSAEHPMNTPIRRWHEDHFKLAEAKLLKETGKTW
                     DETFPQGPYYQLTHIDMPKKHLLLAEAIVGDIDKLKNKRAWISIMPIPYMEVETAWSR
                     VFAWTAPKHMSEEEFFAAMKKSEMIDMTVPFSVQTPQWLNYVPLTVTYTKRVGGQYFG
                     MGRNGSICNASIHLGTHMDGEKHFFPNGRTIGQVPLKDWVGPGVIADISKLVSDSSVY
                     TPQMIMDQVEVREGDILVIKTGWNKYGWNSKDSDEFRYMVKHPGPSPDFSEWAIKMKI
                     KWIGVDAVSADHPMNTIMRIWHPKTFAEANAKLIKDFGKDWDELYPLKDFYQDMHLNL
                     FPHTIVHAENLAGQLATAKSGRYYIGCYLQKAMEAESMWGRFVAFKE"
     gene            12882..13766
                     /locus_tag="PLW25_02395"
     CDS             12882..13766
                     /locus_tag="PLW25_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOS53369.1"
                     /translation="MKPVNFDYYAPSSVDEALDTLAELGYDGKVLAGGQSLIAAMNFR
                     MARPSALVDLNNVAELSYIKPTADGGLAIGTMTRVSTVEHNQDVIQRFPLLVEVAKFI
                     AHPQIRRRGTFGGAIAHADPAGQLPSISMVMNMQALIKGKGTERWVAAPDLIIGPFMT
                     VIEPNEMLAELVIKPHPPRTGAKYVQVSRQSGGYAQSAVASIVTLDENEICKDVHMVL
                     MSVAEVPILSEKAREILIGNKPSKDAIEAVAEAASTSEIDPASDLHASADYRRSITRV
                     LIVRSLTEAFERAKRGGK"
     gene            13766..14248
                     /locus_tag="PLW25_02400"
     CDS             13766..14248
                     /locus_tag="PLW25_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOS53370.1"
                     /translation="MKKLFPITVTVNGKVYERQVEARLLLSDFLRHDLGLLGTHVGCE
                     HGICGACTILFDGEAVRSCLIFAVQANGHEIRTVESLGTPENLHPLQQSFTEKHALQC
                     GFCTPGFLMTLVPFLEENPTPTEDEIREAIDGNICRCTGYEKIVEAVHDAAEKMSQKK
                     "
     gene            14263..16617
                     /locus_tag="PLW25_02405"
     CDS             14263..16617
                     /locus_tag="PLW25_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOS53371.1"
                     /translation="MTGKYFGNPIKRLEDKQLLTGNAKYIDDIEVPGQLHAAFLRSDF
                     AHARILKIDVSEALKRPGVVAVYTAEDFGDFWKPGPLQVPPPTAIPGSKFNARTLVPI
                     AKDKIRYSGEVLAVVIAESRYIAEDAFDDIIVDVESLPAVVDLEKAWDDSSVLVHDDL
                     PSNMAALVTQEVGNYEEAKKKADVVVARRMLIEHVAGAAMENRGFLASWDDQNQVMTI
                     WANTQSPLTVRGSVSRALGLAESQVHVITPAIGGGFGPKVMTSLTDDVLLPWISKQLK
                     RPIKWVEDRRENFLATTSERDQVHHCEIALKKDGTILGFKDVFYHNTGAYDSYGMTVP
                     LNTQTHIISNYRVPNFYTQIRMVFTNQMVVTPVRGAGRSEGVFAMERMLDFAAKEMGL
                     SPIEIRRKNLLRADELPLATGITGQDFVKNTLDSGDYLGNFEKCLQAIEYDKFIKETQ
                     PKAQKEGKRLGIGVVAFTEGTAVGPYEGCKVTVSTSGKVTMTTGYSSQGQAHYTVFAQ
                     IIADQLNVKVEDVKVITGDTNHFAWGAGTFASRGATLVGTAALLASQKVRAKIFSTAS
                     KALGVPEDQVELGEGSVSIKGKPDSAIPLGTLAAMANPMRGVIEPGVEPGLEATAYYG
                     PPHGSTGCGAMGVILSIDEETCKPVLEKMVIVDDCGTMINPLIVDGQLQGGISMGIGN
                     TLYERLVYDENGQLLTASFMDYLMPLATDMPKKLEIIHNHAESSLKLNPLGIKGVGEA
                     GAIPPGPAIVQAIEDAFADKGVEILETNLSPSSIYGYLKKVEHK"
     gene            16680..17135
                     /locus_tag="PLW25_02410"
     CDS             16680..17135
                     /locus_tag="PLW25_02410"
                     /inference="COORDINATES: protein motif:HMM:NF017999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HOS53372.1"
                     /translation="MKVEGEHLFKAPREVVYEMFNDPDALGAAVPGMQKMIKLDESHY
                     EAVLGIRVGPVSANFSGNLSLTDEVPPESVTLVIEGKGGAGFVKGVGYVKFEDLGDET
                     TLLKYTGEANIGGTLASVGQRMIDSVAKSMFKTVFEKLDKILEERWQKN"
     gene            17120..17272
                     /locus_tag="PLW25_02415"
     CDS             17120..17272
                     /locus_tag="PLW25_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53373.1"
                     /translation="MAEELKEAATSAEKPAEPKKKFMEVAEYRMILYVVPVALVLLVL
                     AIILGK"
     gene            17523..18941
                     /locus_tag="PLW25_02420"
     CDS             17523..18941
                     /locus_tag="PLW25_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOS53374.1"
                     /translation="MATTSKVLGYLPQDTPPFGRMLLLGFQHVLTMFPATVLCALLMH
                     FDVSTVLTITGLGTVVALLGSKFAINTYIPLYYGSSFSYIAAVASIIGVLEPDLAFGA
                     PAKPSTIAVATAGFIVTGILNILVGLLIRATGGKKGLDKVLPAEITGSVAVTIGIGLA
                     FTALTMASGTCCGVEPNMPPTLKWWLAAALTFLSAVIFSVYLQGKGFIGMLPILLAMI
                     FGYIVAIPIGLVDFSTIGQSGLLTVPKIVFPVFNNALTATAMIGVGVMAIATIPESTA
                     HLYQISLYVDHLAEEMGREKPYFAKHIGLNLMLDGLNDLVNGLFGSTAGTNYGENNSL
                     MVITRNYSGPALITAGTIAVVLGFIGPLRDAIYSIPTAVTGGLAIYLYGVIGVQGIAL
                     MMAEKVDLYDPGKLAIVALILVVGIGGNIGYGGNLPIPLLRGVFPFGWPAIAAGAVFG
                     MIVNLLFLVWKPPKVRSTDVLE"
     gene            19053..19961
                     /locus_tag="PLW25_02425"
     CDS             19053..19961
                     /locus_tag="PLW25_02425"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HOS53375.1"
                     /translation="MILLTGAAGKTGKTILNRLIPHGVHVRSLVRNEQQAQEIRQLGS
                     TDVAIADLRDQQALSQALDGIHKIYFICPNMTPDEVEIGRSLITLAKQKGIRRFVYHS
                     VLHPQVEEMPHHWQKMRMEECLFKSGLDFTILQPCAYMQNILANWTSIIDEGIYPVPY
                     DTSARISVVDLEDVAAAAEVVLTQMNHSHAIYELAGPQALSQDEMAEVLTQEIGRPVK
                     AVAIDRSDWAVNAQRSGLDENSIDTLLKMFEYYEHYGFQGNPNVLNSLLKKPATKFVD
                     FVKRTHESRLRYGSNGQNGQNHHHRK"
     gene            19965..20456
                     /locus_tag="PLW25_02430"
     CDS             19965..20456
                     /locus_tag="PLW25_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53376.1"
                     /translation="MDKKKSHQWNFAFDAMPALFHSQTDGFVKYLDKDGVKFLKFYWD
                     LLGDKLPEEKRISAAGLTFTKEVIDSKNHLIIITLPSPKEDGDPYFIGLVPKPERRFA
                     IVRFYNSTMFVLLRKDDTDHANRTSFGEVTPKGNYHERGIGLKPTQQDFKRVIKNRLE
                     RKK"
     gene            20453..20860
                     /locus_tag="PLW25_02435"
     CDS             20453..20860
                     /locus_tag="PLW25_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53377.1"
                     /translation="MNSKISTELIVVIVAIVIFYLRVAMLRGQKKRYERDLALKRRKV
                     KGRSKGSPLPQQPKGTPPFTVRSWVLVGISMLLMLAGVVAYNKFYFLGMQLVPDPAFV
                     EAYAKFWYILVSAGVILLAFTVTIRKPIEDSVE"
     assembly_gap    20967..21066
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21150..21419)
                     /locus_tag="PLW25_02440"
     CDS             complement(21150..21419)
                     /locus_tag="PLW25_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOS53378.1"
                     /translation="MILAKVVGTVVTTISHPHYKNRRLLVVQPLVTEGESSEEDFIAI
                     DNTHAGIGDTVLVNREGNGARQVLNNPDACIISVIVGIVDSVTIN"
     gene            complement(21427..21747)
                     /locus_tag="PLW25_02445"
     CDS             complement(21427..21747)
                     /locus_tag="PLW25_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOS53379.1"
                     /translation="MLFGRVHGTAVCTIRVPGTEGLKFLIVQPLNCKLEPLGPLQVAV
                     DVVQAGVGDLCVMVRSREASLAMPEVKFVPVDLALVGIVDELSVKPDGVFDLVMPRGT
                     CEYT"
     gene            complement(21751..22104)
                     /locus_tag="PLW25_02450"
     CDS             complement(21751..22104)
                     /locus_tag="PLW25_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53380.1"
                     /translation="MPKIFFTERDIEEMAARGEMFLEVSDEVVLTELAYEKAQRLGVR
                     LHQTNSLPPAAPIRPYLAQAVNYCPECSTSQKPAEAELRQRIRDAVAARLGTQVDPAL
                     LETIITRVLNNVGMK"
     gene            complement(22130..22756)
                     /locus_tag="PLW25_02455"
     CDS             complement(22130..22756)
                     /locus_tag="PLW25_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53381.1"
                     /translation="MAEDFSLRCYCFIDRMQAQYAAFVGTITQGDLPVEGMAALYVEM
                     APGNEVFRVVDIAVKATEAKPGAQIVEREFGMFEVHSLNQAAVIEAGQVVLDRLGLNK
                     EDRIKPEVVSTQIITRIDPYQSQLINRFRRGSMLVPGETMLVLECAPAAYINYAVNEA
                     EKKANIKIIHVSSVGRFGRMWLSGSEAEIINARNAATSALESLEGIKG"
     gene            complement(22749..23636)
                     /gene="eutJ"
                     /locus_tag="PLW25_02460"
     CDS             complement(22749..23636)
                     /gene="eutJ"
                     /locus_tag="PLW25_02460"
                     /inference="COORDINATES: protein motif:HMM:NF011660.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HOS53382.1"
                     /translation="MTTLKWQTAGADPEGWLSRAEKVIHLNPDGRAPSLTAPPEGNLR
                     VGVDLGTAYTVLVVLDEAGNPLAGEYQFAQVVRDGLVVDFFGAVNLLKTLKDKAERRI
                     GRPLTRAASGYPPGVPRAEVRATTHVVEAAGMVCERLVAEPEAANQVLGIHNGVVVDV
                     GGGTTGIAVLENGKVVYTADEATGGTHFSLVIAGATGLSFDQAEALKKTTSEQAGLFP
                     LVRPVMEKVGTIIARHLRGSAPESITLVGGTALFPGMAEVVQQVTGIPTRVPAHPMFI
                     TPLGIALSDNDTDPRRQDG"
     gene            complement(23652..>23918)
                     /locus_tag="PLW25_02465"
     CDS             complement(23652..>23918)
                     /locus_tag="PLW25_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOS53383.1"
                     /translation="VEAADAMVKAANVVLIGSEYVGGGFVTVMVRGDVGAVKAATDAG
                     AAAAKRVGELVSVHVIPRPHQDVEMILPQSTKGSIGGRGSETKK"
     assembly_gap    23919..24018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24019..25244)
                     /locus_tag="PLW25_02470"
     CDS             complement(<24019..25244)
                     /locus_tag="PLW25_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOS53384.1"
                     /translation="MPDLDQDLLSIQEARNLATRAFEAWKTWAKASQQDVDRVCAAMA
                     AAAFNAADRLGQMAHEETGYGIPAHKRLKNEFAARNVWESIKDIKTAGVIHHDPEKRI
                     YQIAWPMGVIAGLTPSTNPTSTVIYKILIAVKARNAIVIAPHPSAARCSYEAAALMVK
                     AGEENGAPPGLIACMQHVSLAGTQELMGHKYTALILATGGTPMVRAAHSTGKPAYGVG
                     PGNVPAYVDRSADLEKAARYITASKAFDCSTICATEQAVVADRPIARSLQRLMEQEGA
                     YFVDEAQTDLLRRLLFNSEGVMVPATVGKPAPYLAALAGFDVPAGTRVLVTRLNRVGK
                     EEALSREKLTTVLGWYEADGWEAGCERCIELIQFGGRGHSLVIHATDEKVIMAFGLEK
                     PVFRIAVNTMSTLGAIG"
     gene            complement(25252..25542)
                     /locus_tag="PLW25_02475"
     CDS             complement(25252..25542)
                     /locus_tag="PLW25_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOS53385.1"
                     /translation="MLIARVIGTTVSTVKDEKLTGRKLLIVRQTDEHGEAVGKPFVAV
                     DTVDAGVGDLVLTAAGSSARQTAITKDTPVDTVIMAMIDTLEVDGVVTFRKS"
     gene            complement(25552..26478)
                     /gene="deoC"
                     /locus_tag="PLW25_02480"
     CDS             complement(25552..26478)
                     /gene="deoC"
                     /locus_tag="PLW25_02480"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOS53386.1"
                     /translation="MTPDIKTVETLVEIITREVLLAMIEQEERQKNPPGATCVVEVAN
                     GVKVKTCFDNAGHVISAGAERLTSTVGVIPGDSALAGMIDHTLLKPDATSDKIAQLCF
                     EAKKHHFASVCVNPTHVKLCADLLKDSEVKVCTVIGFPLGATSPEVKAFETRNALENG
                     ATEIDMVLNIGALKAGDTNLAARDIRGVVEVAHAANALVKVIIETALLTDEEKVIACL
                     LAKEAGADYVKTSTGFSGGGATVHDVALMRRTVGPSLGVKASGGIHTHEEAEALVAAG
                     ATRIGASAGVKIIRADQSETKPQPAAPEVKAY"
     gene            complement(26475..>26924)
                     /gene="rpiB"
                     /locus_tag="PLW25_02485"
     CDS             complement(26475..>26924)
                     /gene="rpiB"
                     /locus_tag="PLW25_02485"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOS53387.1"
                     /translation="IGTDHGGVELKKVLIQEISHMGFKIMDCGTNTSDPVDYPDIALA
                     VALQVAQGAAWRGVVIDGAGIGSCMAANKVPGVRAALCYDYATAVNSREHNDANVLTL
                     GAGLIGVNLAKQVLKTWLTTEFGGDRHARRVDKIMEIEKKYLSGKTA"
BASE COUNT         6377 a         7379 c         6942 g         6026 t
ORIGIN      
        1 cggtttttca aaaacacttg tattttcgga aagtttatct atacttattg tatatttcag
       61 caattttcag gtcgaaattg ataaaaatcg acgcgattga taagaagata tctgacctct
      121 tactgcaaga cggcaggatg agttgcgcgc aaatcgccaa acgcatcggt ggcgaagttt
      181 ctgaacgcgc ggtacgctac cgcattaacc gcctggtaga aaacggcatc atcagcgtgc
      241 gcggcaatgt caacgctgaa aatattgggc tggtggtctg cgcggatgtg ttcattgaag
      301 tcgaaccgtc gcaaatcaaa actatcgcca tgaaactggc agagtatgag tcaataagtt
      361 acatcgccta cgccactggt gatactgata tcagcataca gctttttacc cctgataaca
      421 gcacgatgtt cgattttgtc actgaggtca tcgggaaaat ccccggggta cgtaaaaccc
      481 acatttcctt tgtgccgatt aagataaaag acgaccacgt ctggccaatc ccttccttta
      541 ttgtgacgca agactgagcc ttaacatcca ttatgggtat gtatttgcca ggactatcta
      601 acgcaggctc cctgcgtttt gattgatttt attcttcagg atcttcacag gaggatacat
      661 ggcaccagag atcgataagg caacgctcgt cagatcagag aagcaaaaat taaaaaggga
      721 gctgaccatt ctcccgttat tcgcgctcat ttacttcacc gtttgcggcg gcgcgtttgg
      781 cgcagaaccc atggtaggtc tctctggccc tgggttggcc ttgctgttga tggtcatcac
      841 cccgctcgtc tttagcatcc ccaacatgtt gatggtgcgt gaaatgcaat ccatgatgcc
      901 agtagaggga ggttattatc actgggtcaa gcaggctttc ggaccattca tcggttttat
      961 gtcaggatgg atgaactggg tggtctcgtg ggtggatgta tccatctacc cggtgtggac
     1021 cgcctggtac ctgagctact ttattcccgc gctcagcgac ggcgccacca tcggcgggat
     1081 tcatttttca tccgaccttc tctcctggct ggtatcagcc gcgctcatca tcaccatttc
     1141 tcttttgaac gttcgcggcg ccaagctctc tggtctcacc gccagttggc tgggcgtggc
     1201 gatgatcatc ccgatcatta tcatgtctgg ttttggcatt tacgcttgga tcgtcaatgg
     1261 cacccccagc aacatcccat tcctcccggg tggcgctgag gtaagtgggc gcaacctgct
     1321 gggggcattg agcgtgggta tttatgtggc catgtggaac ttcatgggtt gggagcttcc
     1381 cactgccgcg ggcgatgaaa ttaaaaaccc caagaagacc tacccgcgtg ccatggtgct
     1441 ggtgcttatc gccaccatcc tttcttactc cgtgccaacg gtagccggcc tgtatggcgg
     1501 agctggagaa gacgggcaat atctggcctg ggggctcgaa gaatccgccc cgggcacagg
     1561 cattggtccc attcttaatg actatggtat cgcggatgaa aaaatgctca cctggggtat
     1621 cgaccccggg tcagatgcgg gctgggagtt tccaacgatt gcccaccgaa ttggcgacaa
     1681 ggtcgcaggc aaagacagcc cgctttccta tttcctcggg gcgattgtag gaatttctgc
     1741 catcttctcc atggttggct tgttcattgg caacggctta ggaggtacgc gggtgcctta
     1801 cgcgatggca gaggacggta tgatgccaac ctggctgaat aaagttcatc caaagtacgg
     1861 cacgccgtgg gtttctattc tcatctgcgg cgcattttac ctgatattct ccacctccgc
     1921 attccagaat ctggtcgtca tcgatgtgtt tctcaacatg ctggtgctga tggctgagat
     1981 tttcgcgctg gtcgttttga ggtacaaaaa accagatatt ccgcgcaaac gggttcctgg
     2041 gggtatatgg gggttgatct acattgttgt ggcgccatta accatcatca tcattgccat
     2101 cgtcagccag attatcgatt acggctggca aggggctgtt gggctggctt taatcgccat
     2161 ggcgattggt gcggtattgt atttcccaat ccgcaaatgg gtaaagaaag acatccctga
     2221 tgtggatccg tatgtgttag aagcagcaga agaagcataa aatcactagt acacaggagg
     2281 ataatatgaa agtattgctg gttggagtgg gtggtgttgg cgaagccatc gcgataatgg
     2341 cggccaaaag accctggttg gaaaaaatgg tgctggctga cttcaacaaa gaacgcgcgc
     2401 tggaagtaca ggccaaaatc aaggacaatg aacgcttccc cgtggaattc gtagatgcgc
     2461 gcaaccgcgg gatgatcgag gagctggcga aaaagcacaa agtagacctg atcatgaacg
     2521 cctgcgaccc gagctttaat gaaaccatct ttgacgccgc gtataatgtg ggcaccaact
     2581 atatggatat ggcgatgacg ctctctcatc cccaccctga aaccccttac acgaagacag
     2641 gcatcaaact gggagattac cagttcgcgc gcgcggataa atggaaagaa aaaggcatcc
     2701 tcgccctgtt ggggttgggt gtagaacccg gcatggcgga cgtctttgcc aagtacgcgg
     2761 ctacccacct ctttgatgaa atcgatgaag tgaatgtgcg cgatggcgcc aacctcattg
     2821 tgaaaggcta tgacttcgcc cccaacttct ccatttggac gaccatcgaa gaatgcctga
     2881 atccgcctgt tatctgggaa aaaggcaagg gctggttcac taccgagcca ttttccgaaa
     2941 aagaggtctt cgacttccca gaaggcattg gccccaacga gtgtgtcaac gtggagcacg
     3001 aggaagtgct gctcgtaccg cgctatatcg attgcaaacg ggtaaccttc aaatacgggt
     3061 taggtgatga attcattggc attctgcaga ccttacacct gctggggctg gacaacaaag
     3121 aacccatcaa ggttgggaaa gtagaggttg ccccgcgcga tgtggtggct gcctgcctgc
     3181 ccgaccccgc ccacctgggc ccgttgatgg aaggacagac ctgcgctgga acctgggtca
     3241 agggaaagaa agacggtaaa gaaaggaatg tgtatattta ccaggtcgcc gataacaaag
     3301 aaagcatgga caaattcgat tgccaggttg tggtgacaca aacagccttc aacgccgtca
     3361 tcggcatgga actgctggca gaaggcatct ggaaagccac cggtgtagaa ggtcctgaat
     3421 tctttgaccc gctgcccttt atggatcgca tggctggaat gggcttcccg tggggcatga
     3481 aggagtggtg agtaatttca tagaatagtg atgatttcta tgaattcccc agccgtaact
     3541 tttgagtcat aataaaaaag tggtttggtt ggggaattct ttttttgccc ggcatccgtt
     3601 tcaaatcgca ggagcgggat gagcatgatt ccatcttcag aaatcaggct caacgcctgt
     3661 ggtcaaactc aaccatctta tcaattccac tcacacaaag gattgtctta tggtcaatta
     3721 tcaagaaaac ctcaaacacc tatcacccgt ttggacgcat cttactgaca tcgttgttga
     3781 ccacgccgaa ggctgttacc tgtacgccac ggatgggcgc aagatgttgg atttcacctg
     3841 cgggattggc gtgaccaaca cgggtcactg ccatcccaaa gtggtagaag ccatccggca
     3901 gcaggccggg ttgctcattc atgggcaggt taacctggtg gtgcataagc cgctgcttga
     3961 actggtagac gagttgcgta cagttcttcc ctccacgctg gacggcttct tcttcagcaa
     4021 ctcaggggct gaagcggttg aaggcgcgct caaacttgcc cgtcatgcca ccgggcgccc
     4081 gaacgtcatc gtcgttcagg gcagcttcca tgggcgtacc aacgccacca tggccatgac
     4141 cacatccaag aatagttaca aaattggcta tcaaccgctg atgccggggg tttacgtcac
     4201 gcccttccca tacgcgctgc gctatggttg ggacgaagaa aaagccagtc agtggtgcct
     4261 ggacgaactg gagctcttgt taatgaccca gctcgctccg caggagacag ccgcgatctt
     4321 catcgagcct attatcggtg aaggcggcta cctggttccg ccaaaaactt ttatgaaagg
     4381 tctgcgcgaa ctgacccaaa aacacggcat cctgttggtg gctgatgaag ttcaatctgg
     4441 ttttggtcgt acaggcaaat ggttcggcta tcagcacttt gaaatcgaac cggatatcat
     4501 gaccgttgcc aaaggcatcg cctcaggcat gcctatttct ggcgtcatta gccggcttga
     4561 gttgatgtcc aaatggatcc ctggatcaca cgggggcacc tatggaggaa acgccgtcgc
     4621 ggctgccgct gcggtggcaa ccatccgcac gataaaagaa gagaaaataa ttgagaacgt
     4681 cgttgcccgc ggcgcccagt taaaagcggg tttggctcac ttgcaggaga aataccccca
     4741 aatcgctgat gtgcgcgggt tggggttgat ggtggggact gaattccgtg atgcgcacgg
     4801 caagcctgat aaaaaaacca ccaaagccgt gcaggctgaa tgcctcagcc ggcagatgat
     4861 gctgctcacc gccggcccct gggacaacac cattcgctgg atacctccac tggtggtcac
     4921 agaatcccag atgaatgaag ccttaaacat ctttgaatca gcactggcgg ctgtcatata
     4981 ataacagtac gtatacatta acaataggag aggttcaatg agtgaattaa tcgaccattt
     5041 gttggcgaaa gcgcgtgaag cgcaaaaagt gatcgagttt tggccacaag aaaaagtaga
     5101 tgagatgatt ctggcagtgg gatgggaagc ctacaaacgc gagaccgctg aaaccgtcgc
     5161 ccgccttgcg gtagaggaaa ccggattggg ggtttatgaa gataaagtcc tcaaacacca
     5221 gaagaaaacc atcggcgtgc tgcgtgattt acaaggagaa aaaacggtag gcgtgatcga
     5281 acgcgtaccc gaaaaaggat tggtgaaaat cgccaaaccc atgggcgtta tcggcgccat
     5341 cacccccatg accaattctt cctccaccat gccctgtaac gggttgatga tcctcaaagc
     5401 cagaaacgcg gtcattttcg ccccgcaccc cagagcaaaa aaaacctgcg cggtgacctg
     5461 tgaagaaatg cgcaaaggtc tggctaaagt cggcgcgccg ctcgacctgg ttcagtacat
     5521 tgaagaaccc agcatcgaac tgacacaaga actcatgagc aaggttgacc tggtgctggc
     5581 tacagggggc gccggggtag tgaaacccgc ctactccagc ggaaaacccg cttacggcgt
     5641 gggcgctggc aacgccactg tggttgtgga tgagaccgct gaccttcctg tcaccgccgc
     5701 gaaaatctgc aaaggcaaga tttttgacaa cgcgacatct tgctcagccg aaaacaatat
     5761 cgtcatccag gaaagcatct tcgatgacct gctggaagaa ttaaagaaaa ccggcggtca
     5821 ccttgtaaca ggagaagacc gcgccaaatt gaaagcggcc atgtggccag atggtgtgca
     5881 tctaagcggc aagatcgtgt gtaaaagcgt caagttcatc gctgaagaag ccggcatcca
     5941 cgtaccagat ggcaccaagt tcatcatggt gctcggggaa cacatcggcc ccgaggatat
     6001 gttctctgcc gaaaaacttt cacctgtgct gaccctgtgg aagtacaaga ccttcgatca
     6061 ggcagtgcaa atggtcatcg atatcaccgg cttcagcggt tacggccatt cttgcggtat
     6121 tcattcagtc aatgaagaac acatcctgga gctggcgacc aaagccaaag tcagccgcat
     6181 gatggtgaga caggctcaga gcgtgggtaa cagcggtgac tgggagaacg gcatgccctt
     6241 cagcatgtcg ctcggttgcg gcacctgggg cggcaacatt accagcgaga acatcacggt
     6301 caaacacttc cttaatgtga cctgggtttc ttaccctatc ccggctgaaa tacctgaccc
     6361 cgaggtcatt ttcgccccgc atttcgcaaa gtacggaaaa tagaacttta ccctcgtaaa
     6421 aaaaagcagc ctttgcggct gcttttttta atccctggct gctgcctcgg cgtggaactg
     6481 ctgataatgg gggagaaagt catacgtggg gtagaactca gtcgtaaagg ctttccaggc
     6541 tttcttctcg acgatcatat ttaattcgcg taagcgagat tgtggaagcc tcagggtgac
     6601 cacgtgcccg atacccatca tcaccaccca ggagacaatt tccattccct ccggcgggaa
     6661 atccctccag aagccatttg ccctcaggtg agcctgtatc tcatccaggc tcttggattg
     6721 gtcatgcctg aaaaagatgg tgagcatctg tgtctcttta tccattatgt ctcctttttt
     6781 ctggtcttgt agtatgagag gggttttcat accgttactg tatcacggtt ttttgaatga
     6841 ctcagccatt tctgcttcgc cgtacggaaa aggtcataat tttcacccga acgatgttac
     6901 attaatcctg ttttcttctt aatcatccgg caattgttga cttcaattgt ccggaatgct
     6961 atactctaag caacaaaata ttataagaaa aggatgcaaa atgaaaatag aattcccgac
     7021 tcctgtttca actgaagaaa tgaaaatcga tctgcgcgag atcgaaggca ttcttcaggg
     7081 cgccccaatg gatatgcccc ccatcccgga agccacagtt accttaaaag acggtcgtat
     7141 cgtttacatc cgcggtatca aggatagcga catcgatccc atgcttggct acctcgaaaa
     7201 ggtcatgaaa gttgataagg acttctatga tattgtgggt gtacgtgttt atggtgaggt
     7261 tctcgccctg cgccgcaaac gcctcaaaga tcctttcacc atggtcggcc tggtcgatgg
     7321 cgaatggctc ggtttcgcca atggtcgcgc ttgggataaa gacatcgcca tcagcctgca
     7381 cacactcacc ttttctcgta aagcccgcct gggctggccg atgtactacg ccaagaccta
     7441 ttacgccatg gaattgctca aggtcaagga atggtggtca actttcgaaa gctacaacgg
     7501 ctggcgtatg gcaggccttt caatggctca gcctaccaaa ccctggccag agtaccagca
     7561 cgagctgggc ggcgccaagg tcttctatct taaccaggat atgtggcata gccgcgtaaa
     7621 agactatgtc cgcgaaatcg tcggtcagga tatggacttc aacgtaactg atgaagttcg
     7681 caaagccaac gaaaacttcc gcgtaccgga gactgtcgac ctgtaagtta ggagtcttat
     7741 gaatttgaag aacctcctct gtgcagcgta tcaaatgagg aggttctttt atttgggaaa
     7801 taatgttatt tgtcattttt tttacgatta aggtaagttg actggcttga ttttttcgtg
     7861 atatattgaa gaaggtgaac catggggtca gattgttttt ccccatccac tcgccgttca
     7921 aagcaagttc tttatcatcc aagggaacgg tgcacaaatc gatcgcggtt ttctgaatac
     7981 gcatctgcgt gtgtcacccc ctaataccca ttctatgaag gagcgattat ggcaacattt
     8041 aaaacagaag tccgtagtgg tgtttattat gactcggcca aactcatgtc tttgcaacgt
     8101 tcgcttgccg ggttgcctgg cgttttagat gccggcgtgg tgatgggaac agattcaaac
     8161 aaagaattac tggcccatat cggtttagac acccctgaag taaaggccac caaacctgac
     8221 gacctggcca ttgtgatcaa agctgagaat gaaaaagccg ctttggacgc gctcgcccag
     8281 gtggatggat tgctggctgc ccgtaaaggc ggttcagaac aggaatatca gccgcggagc
     8341 attgaaagcg ccgctgatat gctgcccgac gcagaatggg tcatcgtttc agtcgctggt
     8401 cgctatgcga ccgccgtcac ccgcgaagcg ctgcggctgc gcaagcatgt gcacctcttc
     8461 agtgacaatg tcagcgttga aaatgagatc gaattaaaga aagaagcccg cgacctgggt
     8521 ctattagtga tgggacctga ctgcggcacc gccatggtcg atggttatgg gctggctttt
     8581 gccaacaaga ttcgcaaagg acccattggc attgttgccg ctgctggcac cggtttacaa
     8641 caggtagcca cccgtattca ccagttgaac agcggtatca ctttcggcat cggcaccggc
     8701 ggtcgcgacc ttaaagcaga agtgggcgcg atcacctttt taatggcact ggacgtgctg
     8761 gcgagagacg acgataccaa ggtgatcgtt cttacctcca aaccaccttc acccaaagtg
     8821 gctgaagaag tgctgaaggt cgcccgcaaa gccggcaaac cagtcgtggt gaacttcatc
     8881 gcgcggccaa tcaccaccac gcgtgacggc aacctgttct tcgccaccgg cttggatgac
     8941 gccgcccgac tggcggtgga gctggcacac aacccgccca agctcgtcag tgatgctgac
     9001 ctgccgatta aaaagttcac cgccaaacag aaatacctgc gcggtttatt ctctggcggc
     9061 acactggctt acgaagccca gcacatcctt tacggctaca tccccagggt gctggcaaac
     9121 tcacccatta ataaagaatt ccagctgccc gacgcgttcg tgagccagga gaactgcatc
     9181 gtcgacctcg gcgaagacga attcacacag ggccgcctgc acccgatgat ggataacgaa
     9241 ctgcgcattc gccggttgta tgaagaagcc aaagaccccg aagtggcggt tattttgttg
     9301 gatgtggtga ttggttacgg ctcacatccc gaccccgcca gcgagatcgc tccagccatc
     9361 gccaaatgca tcgctgatgc caagaaagat ggacggcacc tggaagtggt ttgcgtggtc
     9421 tgcggcacag atgaagaccc ccagaacctg aacaaccaga tggcccagct aaaagccgcg
     9481 ggcgccctgg tagataccag caacgaggtc atctgccgct acgtcggtcg catcttgcgc
     9541 gcgttggctg agcgcgatga gcccaaagcc cacgcgcttg ggaaaccggt tgacctcgaa
     9601 aaaatcaaaa aaccgttagc tgccattaat gtgggtcttg aatccttcgc tgccaacctg
     9661 aaagatcaag gcgcgcctta tgtgcaggtg gattggaagc cgcccgccgg tggtaacgaa
     9721 aaattgatga atattctgca aaagatgaaa tcacccagaa agtagttcct gataatccat
     9781 aatgaccctg gaggaagcaa atggttgata tcgaaaaagc aaacgcacta gcagtagaaa
     9841 aattcatgga agcccgcccc gtcgccatca cgctggcgaa ggcgctggaa atcgtaccgg
     9901 gaatgcacga aaacatgctc ttgcacgccg gcccgcccgt gacctttgaa agaatgtccg
     9961 gcccgaccaa gggagccatg attggcgcct tgctctttga aggcaaagcc aaaaaccccg
    10021 aagaagccga aaaattatta aaatccggca agatcgaatt ctccccctgc catgagcata
    10081 tgtgcgccgc accaatggca ggcgtcattt caccttcaca gctggtgtat gtgatcgaaa
    10141 accagaccca cggcattaaa tgctactcca acctcaacga aggccgtgga aaagtgctgc
    10201 gcatgggcgc gtacagcgag gatgtgctgg aaaaacttcg ctggatggag gctgttctgg
    10261 gcccaaccat taaaaccgcg ctcgaagcga tgggcggtat cgatgtgcgc gccatccttg
    10321 ccaaagcgct gcacagcggt gatgatgggc acaaccgcct ggataccgct tcacttttat
    10381 ggacaaccca actggcgcct tacatcgcca aaacagccaa gaatacagaa accgccttcg
    10441 aggtcacaaa attcctgggc gagaacgcgc tgagcattct caacccggtg atggctggat
    10501 gtaaaaccat gaccgctgcc ggtcacaaca ttgaaggcag caccatcgtc accatcatgt
    10561 cgcgcaacgg caccgattgg ggcatcaaag tgagcggcct gggagacaaa tggttcaccg
    10621 cgccctcgcc tgtggtcaaa gccctttact tccctggctt cactgaaaaa gatgcctgcc
    10681 cggatatcgg cgacagcgtg atcaccgaaa cagccggcat tggtggcttt gccatggcaa
    10741 ccgcgcccgc ccttgtcacc tttgttggcg gtgaaccgcg cgatgcgatc aacaccacgc
    10801 tggatatgta cgaaatcact gaaggagaac acaagcagtt caccattccc ttccttgatt
    10861 tccgcggcac ccccactggc gtggacatcc gcaaagtggt cgagaaacaa atcaccccgc
    10921 gagtcaacac cggtgtggcg cataaagacc caggtgttgg gcaggttggc gctggcgtcg
    10981 tttcagcccc gatgaaatgt ttcgaagacg cgctaattgc cttcgcagac aaatacgggt
    11041 ataactaatc caaacatatt aaaaaatgga ggaaaaatga atcgagaaga ttttatcaaa
    11101 atgctcaccg atgccgagct ttatgacctc acgcaggact gcagcatttt caccccccct
    11161 cacccgcatg aaaaagggct tgaggtgcat ttcttcaagc gtgttaccgg cgcctatggt
    11221 ggcggtcagg gcgccaatgg tcaaatcctc aactggagca ataccgttgg cacccacctg
    11281 gtgggcgaac gggcgttcaa ttccggcggt cgcgccattg cggatatccc gctcaccgac
    11341 ctgtgcgggc ccggcgtagt ggcagatatc tctgacctgg tttctgatta cagtatttac
    11401 acccctgaaa tgatcacttc caaggtcgag gtcaaaaagg gtgacatcct catcgtcaac
    11461 accggttacc acaagtattc ctgggaccag ccggcggtgc gcaataaaac cgcccagggc
    11521 ggtgttgaaa gcatggagtt cggtttcctc gtccgccacc cgggtccttc aatggacttt
    11581 tacaagtggg ccatggatat ggaactcaag gtcatcggcg tggactgcgg ttcagccgag
    11641 caccccatga acacacccat ccgccgctgg catgaagatc atttcaaact ggctgaagcc
    11701 aaattactca aagaaaccgg caaaacctgg gacgagacat tcccacaagg accttattac
    11761 cagctgaccc acatagacat gccgaaaaag caccttttgc tggcagaagc tattgtcggc
    11821 gatatcgaca aactcaagaa taaacgcgcc tggatcagca tcatgccgat cccttacatg
    11881 gaagtcgaga ccgcctggag ccgtgttttc gcctggaccg cccccaagca catgagcgag
    11941 gaagaattct ttgccgcgat gaagaaatcg gaaatgatcg acatgaccgt gcccttcagc
    12001 gtgcaaaccc cgcagtggct caactacgtg cctctcacag ttacctatac caagcgcgtc
    12061 ggcgggcaat actttggcat gggccgcaac ggctccatct gcaacgccag catccacctc
    12121 ggcacgcata tggatggtga aaaacacttc ttccccaacg gtcgcaccat tggccaggtt
    12181 ccccttaagg attgggtcgg ccccggcgtg atcgccgaca tttcaaagct tgtgtccgat
    12241 tcaagtgttt acaccccgca gatgatcatg gaccaggtgg aagttcgtga aggcgacatc
    12301 cttgtcatca agactgggtg gaacaaatat ggctggaaca gcaaggattc tgacgagttc
    12361 cgctacatgg tcaagcaccc gggtccttca cctgatttct ccgaatgggc gatcaagatg
    12421 aaaatcaagt ggatcggcgt ggacgcagta tcggcggacc accccatgaa caccatcatg
    12481 cgcatctggc accccaagac ctttgctgaa gccaatgcca agctgattaa ggacttcggc
    12541 aaggattggg atgagctcta cccgcttaaa gacttctacc aagatatgca cctcaacctc
    12601 ttcccgcaca ccattgtgca cgcggagaac ctggctggtc agctcgccac cgccaagagc
    12661 gggcgctact acatcggttg ctacctgcag aaagccatgg aagcagaatc catgtggggc
    12721 cgctttgtag cctttaagga atagcagttt gacgatcgaa ccttcagggg agtaactccc
    12781 ctgaaggttc caacttttca tgtgatacac tttttaccgt caaaatacct ctttgaaacc
    12841 ccaatatttt gaatcctcaa tttaacagga ggttgtaatt aatgaagcca gttaattttg
    12901 attattatgc accctccagc gttgatgaag cgttggatac actggcagaa cttggttacg
    12961 atggaaaagt cctcgccggc ggtcaaagtt tgatcgcggc gatgaacttc cgtatggcga
    13021 gaccaagcgc gctggtggac ctgaataatg tagcggaact ttcctacatt aaaccaactg
    13081 cggatggcgg tttggcgatt ggcactatga cccgcgtcag cactgtggaa cacaaccagg
    13141 atgtcatcca gcgtttcccg ctgctggtcg aagtcgccaa gttcatcgcc catcctcaga
    13201 ttcgccgccg cggtactttc ggcggggcaa tcgcccacgc tgaccccgca gggcagctgc
    13261 ccagcatttc aatggtcatg aacatgcagg cgctcattaa gggcaagggt actgaacgct
    13321 gggtcgcagc gccagacctg atcatcggac cgttcatgac cgtcattgag cccaacgaga
    13381 tgctggcaga actggtcatc aaacctcatc ctccgcgcac cggcgccaag tacgtccagg
    13441 tatcccgaca gagcggcggt tacgcgcaat ctgcggtcgc atctatcgtc accctggatg
    13501 aaaacgaaat ctgcaaggac gtgcacatgg tgttgatgag tgtggctgag gtccccatcc
    13561 tatcagaaaa agcgcgcgaa atcctcatcg gcaataaacc cagcaaagat gcgattgaag
    13621 cagtggctga agccgcctca accagcgaaa tcgacccggc ctcagacctg cacgccagcg
    13681 ctgattatcg tcgcagcatc acccgtgtat tgatcgtacg cagccttact gaagccttcg
    13741 aacgcgcgaa aagaggaggt aaataatgaa gaaactcttt cccattaccg taactgttaa
    13801 cggaaaagtc tatgaacgcc aggttgaagc ccgcctgctt ttaagtgatt tcttgcgcca
    13861 tgacctgggt ctgctgggaa ctcatgtggg ctgcgaacat ggcatttgcg gtgcctgcac
    13921 catcctcttc gatggcgaag ccgtccgctc ctgccttatt ttcgcggttc aggctaacgg
    13981 tcacgaaatt cgcaccgtgg aatcgcttgg aactcctgaa aacctgcacc cgctgcagca
    14041 gtccttcacc gaaaaacacg cgctgcagtg cggtttttgc acccctggtt tcttgatgac
    14101 cctcgtcccc ttcctcgaag aaaaccccac acccacagaa gacgagatcc gcgaggccat
    14161 tgatggcaac atctgccggt gcacaggata tgagaaaatc gtcgaagccg tgcatgacgc
    14221 ggccgagaag atgtctcaga aaaagtgaag cgaggtgata ctgtgactgg aaaatatttc
    14281 ggtaatccaa tcaagcgctt ggaagataag caactgctga caggcaacgc caagtacata
    14341 gacgatattg aagtccctgg tcagcttcac gcggctttct tgcgcagtga tttcgcccac
    14401 gccagaattc taaaaattga cgtgagtgaa gcattgaagc ggccaggggt tgtggctgtt
    14461 tacaccgccg aggattttgg tgatttctgg aagcctggcc ccttacaggt gcccccgcct
    14521 accgccatcc ctggttccaa gtttaacgcg cgcacgctgg tgcccatcgc caaagacaag
    14581 atccgctatt caggtgaagt actggcggtg gtgatcgcag aatcgcgcta tatcgccgaa
    14641 gacgccttcg acgatatcat cgtggatgtt gaatctctgc ctgccgtggt cgatctggaa
    14701 aaagcctggg atgacagctc tgtactggtt catgatgacc tgccctccaa catggctgcc
    14761 ctggtaaccc aagaagtggg caattacgaa gaggcgaaga aaaaggccga tgtggtggtt
    14821 gccaggcgaa tgctgatcga acacgttgcc ggcgctgcca tggaaaaccg cggtttcctc
    14881 gccagctggg atgaccagaa ccaggtgatg accatttggg cgaatacgca gtctccgctg
    14941 accgtgcgcg gctctgtatc ccgcgccctg ggtctggctg agagccaggt gcacgtgatc
    15001 accccggcaa ttggcggcgg ttttggccct aaggtgatga catcgctgac tgatgatgtg
    15061 ctgctgccct ggatatccaa acaactgaaa cgcccaatta aatgggtcga agatcgccgc
    15121 gagaacttcc tcgccaccac ttctgaacgc gaccaggtgc atcactgcga aatcgccctc
    15181 aagaaagacg gcaccatcct ggggttcaaa gatgtcttct accacaatac cggggcttat
    15241 gactcctacg gtatgactgt gccgctgaac acccaaacgc acattatcag caactaccgg
    15301 gtgcctaatt tttacactca aatccggatg gtctttacca accagatggt agtcaccccc
    15361 gtgcgcggcg ccggacgttc agaaggcgtg ttcgccatgg agcgtatgct ggatttcgcc
    15421 gcgaaggaaa tgggcctttc gccaatcgaa attcgccgga aaaacctttt acgggcggac
    15481 gaacttcccc tggcaaccgg tatcaccgga caggattttg tgaagaacac gctggatagc
    15541 ggtgattacc tcggcaactt tgaaaaatgc ctgcaggcga ttgagtacga taaattcatt
    15601 aaagaaaccc agcccaaagc ccaaaaagag ggaaagagac tgggcatcgg tgttgtggct
    15661 tttaccgaag gaaccgcggt tggtccttac gaaggctgca aggtaacggt gagcaccagt
    15721 ggaaaggtca ccatgaccac cggttacagc tctcaaggtc aggcgcacta cactgtgttt
    15781 gctcaaatca tcgcggacca gttaaatgtg aaggttgaag atgtgaaggt catcaccggc
    15841 gatacaaacc acttcgcctg gggcgcgggt acgttcgcca gccgtggcgc caccctggtg
    15901 ggcacggccg ccctgctggc cagccagaaa gtacgcgcaa agatcttctc caccgccagt
    15961 aaagccctag gggtacctga agaccaggtc gagctgggtg aaggcagcgt cagcatcaag
    16021 gggaaacccg actccgcaat tcccctgggc accctggcag ccatggccaa ccccatgcgc
    16081 ggtgtcattg agcctggcgt tgaaccaggg cttgaagcca ccgcgtacta tggtcctccg
    16141 catggctcca ctggttgcgg cgctatggga gtgatcctga gcatcgatga agagacctgc
    16201 aaaccagtcc ttgagaagat ggttatcgtg gatgactgcg ggaccatgat caaccccttg
    16261 atcgtggatg gtcagcttca gggcggcatc tccatgggta tcggcaacac tctgtacgaa
    16321 aggctggtct atgacgagaa tggtcaattg ctgaccgcat ccttcatgga ctacctgatg
    16381 cccctggcaa cagacatgcc caagaaactg gagatcatcc acaatcatgc tgaaagttcg
    16441 ctgaaattaa accccctggg catcaagggt gttggagagg cgggtgcaat tccgccgggt
    16501 ccggcgatcg tccaggcgat tgaagacgcg ttcgccgaca aaggtgttga aattctcgaa
    16561 acaaacctga gtccaagcag catctacggc tatcttaaaa aagttgagca caagtaattc
    16621 aggtgtacaa ttaagtatta cctgtaagtg aagcgaaacc aaataggagg aagtgtgtta
    16681 tgaaagttga aggtgaacat ctgtttaaag cgccccgtga agttgtgtac gagatgttca
    16741 atgatcctga cgcgctggga gccgcggttc ctggcatgca aaaaatgatc aagcttgacg
    16801 aatcccatta cgaagccgtg ctgggcattc gggttggtcc ggtttctgcc aacttctccg
    16861 gtaatctcag cctcaccgat gaggttcccc ctgagagtgt caccctcgtc atcgagggca
    16921 aaggcggagc gggttttgtc aaaggggtcg gttatgtgaa gtttgaagac ctcggcgacg
    16981 aaaccacctt gttgaaatac acaggtgagg ccaatattgg tggtactctt gccagtgtgg
    17041 gacagcggat gatcgacagc gtcgcgaagt ccatgttcaa aactgtgttt gagaaactcg
    17101 acaaaatttt ggaggaaaga tggcagaaga actgaaagaa gcagcaacaa gcgcagaaaa
    17161 accagcggaa ccaaagaaaa agttcatgga agtcgctgaa taccgcatga tcttgtacgt
    17221 tgtgccagtt gcgctggtgc tccttgtgct ggcgataatt ttaggcaaat aagaaaacca
    17281 gcccagtggg ggagagcaat ctcccccttt caccatcttg acaaaatgat gtattattaa
    17341 catatgtagt tttcttcaag atggaataga taacttaata aggaggtgcg tatcggagag
    17401 cgtattttgc gtttacgggg cgagcgaaaa gacatttatc ttttttcgtt ttgagctcaa
    17461 tccaggtaag tagtaatcga tcgaagaaaa ccaattaatc aaaggaaaga aggataaaac
    17521 ctatggcaac tacttctaaa gtgctgggtt atctaccaca ggataccccg ccgtttggca
    17581 ggatgttatt gctcggtttt cagcacgtgc tgaccatgtt cccggccacc gtgctgtgcg
    17641 ccctgctcat gcactttgat gtgtcaaccg ttttaaccat caccggtctt ggcactgtcg
    17701 tcgcattact tggatcaaaa ttcgcgatta atacctatat tcccctgtac tacggctcaa
    17761 gcttcagcta tatcgcagcc gtggcctcca ttatcggcgt cttggaacct gacctggctt
    17821 ttggcgcccc cgccaaacct tcaacgatcg ccgtcgctac cgccggcttc atcgtcactg
    17881 gtattctcaa catcctggtc ggtctgctca tccgcgcaac cggcgggaag aaaggcctcg
    17941 ataaggtttt gcccgctgaa atcaccggtt cggtcgccgt caccatcggt atcggcctcg
    18001 cctttaccgc cctgaccatg gcctccggca cctgctgcgg tgttgaaccc aatatgcccc
    18061 caacgctcaa gtggtggcta gcagccgccc tcaccttcct gtcagcggtc atcttctccg
    18121 tctacctgca gggtaagggt ttcatcggca tgctccccat cctcctggcc atgatcttcg
    18181 gctacattgt cgccattccc attggcctgg tcgatttctc gaccattgga caaagcggtc
    18241 tgctgactgt acccaagatc gtgttcccgg tctttaataa cgcgcttacg gcaacagcga
    18301 tgatcggggt aggtgtgatg gccatcgcca ccattccaga atccaccgcc cacctctatc
    18361 agatcagcct gtatgttgac cacctggcgg aggaaatggg acgcgaaaaa ccgtatttcg
    18421 ccaaacatat tggcctcaac ctgatgctcg atgggctgaa cgacctggta aacggtctct
    18481 ttggctccac cgctggcacc aactacggtg agaacaactc gctgatggtg atcacccgca
    18541 actactccgg cccggcgctt atcaccgctg gcaccatcgc cgttgtcctc ggcttcatcg
    18601 gcccgctgcg cgatgccatt tacagtatcc cgactgcggt caccggcggc ctcgccatct
    18661 acctgtatgg tgtcattggc gtgcagggca tcgccctgat gatggcagaa aaagttgacc
    18721 tgtatgatcc gggtaaactg gcgattgtcg cgctcatcct cgtcgtcggt atcggcggca
    18781 acatcggtta cggcggcaac ctgcccatcc cgctgctcag gggtgtcttc cccttcggct
    18841 ggcccgcgat cgcggctggc gccgtcttcg gcatgatcgt caacctgctc ttcctggttt
    18901 ggaaaccgcc gaaggtgcgc tcaactgacg ttctggagta accctccgaa aacagcacga
    18961 taaaaaactc cccggggaaa aataccgggg agtttttcta tttcatcgat tataatcagg
    19021 aaatcatcct tcattggcga aaagagaaac cattgatctt actcactggc gcagccggga
    19081 aaaccggtaa aaccattcta aacaggctca ttcctcacgg tgtgcacgtg cgcagcctgg
    19141 ttcgtaatga gcaacaagct caggaaattc gccaactggg cagcaccgat gttgccattg
    19201 ctgaccttcg cgaccaacag gcgctttcac aggcgctcga tggcattcac aagatctact
    19261 ttatctgccc aaatatgacc cctgacgagg tggaaatcgg tcgttcgctc attaccctgg
    19321 ccaagcaaaa gggcatcagg cgctttgttt atcattctgt gctgcatccg caggtggaag
    19381 agatgcccca tcattggcaa aaaatgcgca tggaggagtg tctcttcaaa tctggcctgg
    19441 atttcactat cctgcaaccc tgcgcctata tgcagaacat cctggcaaac tggacatcta
    19501 tcattgatga agggatctac ccggtacctt acgacacctc tgcccgcatc agcgttgttg
    19561 acctggagga tgtagccgcc gcagccgaag tagtgctcac ccagatgaac cattcccacg
    19621 ccatctatga gctggcagga ccgcaagcgc tcagccagga cgagatggcg gaagtgctga
    19681 ctcaggagat cggtagaccg gtgaaggcgg tcgccattga tcgatcagac tgggcggtca
    19741 acgcgcaacg ttcaggtctt gatgaaaact cgatcgacac cttgctgaaa atgttcgagt
    19801 attacgagca ttacgggttt cagggcaacc caaacgtgct caactccctg cttaagaaac
    19861 ccgcgacaaa atttgttgat tttgttaaac gcacccacga gtcacgatta cgctatggtt
    19921 ctaacggtca gaacggtcaa aatcatcatc acaggaagta ggaaatggat aaaaagaaaa
    19981 gccatcaatg gaattttgcc ttcgacgcta tgcccgccct gtttcacagt cagacagacg
    20041 ggtttgtaaa ataccttgac aaagacgggg tgaaattcct gaagttctac tgggacctcc
    20101 tgggggataa attaccggaa gagaaacgca tctcagcggc cgggctcacc tttacaaagg
    20161 aagtgatcga ctccaagaac cacctgatca tcatcacctt gccttctccc aaagaggatg
    20221 gcgaccctta ttttatcggg cttgtcccca aacctgagcg gcggttcgct attgtgaggt
    20281 tttataactc caccatgttc gtgctgctgc gtaaggatga tacagaccat gccaatcgca
    20341 ccagtttcgg cgaggtaacg ccaaaaggga attaccatga acgcgggatt gggttgaaac
    20401 ccacccagca agactttaag cgggtcatca aaaatagatt ggagcgtaaa aaatgaatag
    20461 taaaatttcg actgaactga tcgtcgttat cgtcgccatt gtcatttttt acctgcgcgt
    20521 ggccatgctc aggggtcaaa aaaagcgcta tgaaagggat ttggcgctca agcgccgaaa
    20581 agtgaaaggc agatcaaaag gttctccgct gccgcagcag cccaaaggca ccccgccgtt
    20641 taccgtgcgc agttgggtgt tggtggggat ctccatgctg ctgatgctcg ctggggtcgt
    20701 cgcctataat aaattttatt tcctgggtat gcagctggtg ccagaccccg cgttcgtaga
    20761 agcctatgcg aaattctggt acattctcgt atctgccggc gttattctgc tggcatttac
    20821 cgtcaccatc cgtaaaccca tcgaagattc ggtagagtga tacgatgaag gttcgtgttg
    20881 tctctttcat cctgatggtt ctcctgctca cctctttgaa cgcctgcgca aaggcagccg
    20941 ttcacacgcc cacggctgca gttgaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21061 nnnnnnagcc gtctggtcgc ctggcatgtc agcgccatcg accaatcgac tcgcgcatac
    21121 ccgctcttct gttgttgtaa accttggggt cagttgatgg tcactgaatc cacaatcccc
    21181 acgatcaccg agatgatgca ggcatcaggg ttgttcagca cctggcgcgc cccgtttcct
    21241 tcgcggttga caagcacagt gtcgccaata ccagcgtgcg tgttgtcaat ggcgataaaa
    21301 tcttcctcgc tgctctcgcc ctccgtgacc aatggctgaa ccaccaacag cctgcgattc
    21361 ttgtagtgtg ggtggctgat ggtggtaacc acagttccga cgacctttgc cagaatcata
    21421 atccgcctaa gtgtactcgc aagtgcctct cggcatcacc aggtcaaata cgccatcagg
    21481 ttttacgctc aactcatcca caatgcccac cagcgccagg tcaaccggca caaacttcac
    21541 ctccggcatc gccagagagg cttcacgcga gcgcaccatc acgcacaggt ctcccactcc
    21601 cgcctgcacc acgtcgaccg ccacctgtaa ggggcccagc ggttcaagct tacagtttaa
    21661 gggttggact atcaggaact tcaagccctc cgtgcccgga actctgatcg tgcaaacagc
    21721 cgtaccgtgg acgcgaccaa aaagcatggg ttacttcatc cccacattgt tcagcacacg
    21781 ggtaataatc gtctccagca gcgccgggtc tacctgtgtg cccagccggg cggccaccgc
    21841 atcgcggatg cgctgcctca gttcagcttc ggcgggtttc tgcgatgtgc tgcattcagg
    21901 gcagtaattt actgcctgcg ccaggtaagg acgtatcggc gcggcgggag ggagcgaatt
    21961 tgtctggtgc aggcgcacgc ccaggcgctg agctttttca tacgccagtt cggttaacac
    22021 cacttcgtcg ctgacctcca ggaacatttc gccgcgggca gccatttctt ctatatcacg
    22081 ttctgtaaaa aatattttcg gcatcactcg ctcctgcttt ttttctacgc taccctttga
    22141 tcccttcaag gctttcgagc gcgctggtgg cggcatttcg ggcgttaatg atctccgcct
    22201 cgctgccgct cagccacatc cggccaaatc ggccgaccga agagacatgg atgatcttaa
    22261 tattcgcctt tttttcagcc tcattaactg cataattaat gtaggcagcc ggagcacatt
    22321 ccagcacaag catcgtttct cccggcacca gcatactgcc gcgccgaaag cggttgataa
    22381 gttgcgactg gtagggatca atgcgcgtga tgatttgcgt cgaaaccact tcaggtttaa
    22441 tgcggtcttc tttgttcaat cccagcctgt ccagcaccac ctgcccggct tcaatcaccg
    22501 ccgcctggtt gagagaatgc acttcgaaca tgccaaattc gcgttccaca atttgcgcgc
    22561 ctggtttggc ttccgtggcc ttcaccgcga tatccacaac gcggaatact tcgttgccag
    22621 gcgccatctc aacataaagc gcagccatcc cctccaccgg caggtctcct tgggtgatgg
    22681 tgcccacaaa agcggcgtat tgtgcctgca tgcggtcaat gaaacaataa catcgtaatg
    22741 aaaaatcctc agccatcctg cctccttgga tcagtatcgt tatccgaaag cgcgattccc
    22801 agcggggtaa tgaacatggg gtgcgctggc acccgggtcg gaatgcctgt aacctgctga
    22861 acaacctcag ccatgcccgg gaagagcgcc gtgccaccta ccagggtgat ggactcaggc
    22921 gcgctgccac ggagatggcg tgcaataatg gtgcccactt tttccattac tggccgcacc
    22981 aaggggaaaa ggccggcctg ttcagaggtt gtctttttta gagcctctgc ctggtcaaag
    23041 ctcaaaccgg tggccccggc aatgaccaat gaaaaatgcg tcccgccggt agcctcatct
    23101 gcggtataca ccaccttacc attttccagc accgcgatac ccgttgtacc gccgccaaca
    23161 tccaccacca cgccattgtg gattcccaaa acctggttgg ctgcctcagg ttccgctacc
    23221 agacgctcac agaccatacc ggcagcctcc accacatggg tggtcgcgcg cacctcggca
    23281 cgcggcactc ccggtgggta gccgctggcg gcccgggtga gcggtcggcc aatcctccgt
    23341 tcggctttgt ctttcagtgt tttcagcaga ttcaccgcgc caaaaaaatc caccaccaat
    23401 ccatcgcgca ctacctgggc gaactggtat tcaccggcaa gcggatttcc cgcttcgtcc
    23461 agtaccacca gcacggtata cgcggttccc aggtccacgc ccacgcgcag gttcccttca
    23521 ggcggcgcgg tcagagatgg ggctctgcca tccgggttga gatgaatgac tttttccgcg
    23581 cgcgaaagcc aaccctccgg gtcagccccc gcggtctgcc attttaaggt tgtcacaggt
    23641 tgaaacccgg tttacttttt ggtctcagac ccgcgaccgc caatgctgcc tttggtgctc
    23701 tgtggcagga tcatttcaac atcctgatgc gggcgtggaa tgacatgcac cgaaaccagc
    23761 tcgcccacgc gtttggcagc cgctgcgccg gcatctgtag ccgcttttac cgcacctacg
    23821 tctccgcgca ccattacggt cacaaaaccg ccgcccacgt attccgaacc gatgaggacg
    23881 acattcgctg ccttgaccat ggcgtcggca gcttccacnn nnnnnnnnnn nnnnnnnnnn
    23941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24001 nnnnnnnnnn nnnnnnnncc aatggcgccc aacgtcgaca tggtgttcac cgcgatgcgg
    24061 aaaaccggct tttcgagccc aaacgccata atgacctttt catcagtggc gtgaatcaca
    24121 aggctgtgcc ccctgccgcc aaactggatc aattcgatgc agcgttcaca gccggcttcc
    24181 cagccatccg cttcatacca gccaaggacg gtggtgagct tttcacgaga aagcgcttct
    24241 tctttgccca cgcggttcag cctggtcacc agcacacgcg ttcctgcggg tacatcaaag
    24301 cctgccagcg ctgccaggta aggcgctggt ttacccaccg tggcaggaac catgacgcct
    24361 tctgaattga agagcagcct tcgcagcagg tcagtctgcg cctcatcaac aaaatacgcg
    24421 ccttcctgct ccatcaaccg ttgcaaagaa cgcgcgatcg gccggtctgc caccacagcc
    24481 tgttcagtgg cacaaatggt ggagcaatcg aaagctttac tggcggtaat atagcgggcg
    24541 gctttctcca ggtcagcgga gcggtccaca taagccggca cattgccggg tcctacgcca
    24601 tatgcaggtt tgccggttga atgcgccgcg cgcaccattg gcgtgccgcc ggtagccagg
    24661 ataagcgctg tatacttgtg acccatgagt tcctgcgttc ctgccaggct gacgtgctgc
    24721 atacaggcga tcaaacccgg cggcgcgccg ttctcttcac ccgcttttac cattaaagcg
    24781 gcggcctcat aactgcaccg cgccgcggag gggtgcgggg caatgacaat ggcattgcgc
    24841 gctttaaccg cgatgagtat tttatagatg accgtagaag tcggattggt actgggggtc
    24901 aaaccagcaa tcacgcccat cggccaggca atctggtaaa tacgtttttc cgggtcatgg
    24961 tgaatgacgc cggctgtctt gatatcctta atactttccc atacattcct ggcagcgaat
    25021 tcattcttaa ggcgcttatg cgccggaatg ccgtaaccgg tctcttcgtg tgccatttga
    25081 cccagacggt cagcggcgtt aaaagcagcg gcggccatgg cagcacaaac cctgtccaca
    25141 tcctgttgtg aagctttcgc ccaggtcttc caggcttcga acgcccgggt ggcgaggtta
    25201 cgcgcttctt gaatcgagag aaggtcctgg tcaaggtccg gcattgttcc ctcagctctt
    25261 gcggaaagta accaccccgt cgacctccaa tgtgtcgatc atcgccatga tcaccgtgtc
    25321 taccggggta tctttggtga tcgcagtctg gcgggcgctt gagcctgccg cggtgagcac
    25381 caggtcgccc accccggcgt caacagtatc caccgccaca aatggtttgc caactgcttc
    25441 gccatgctcg tctgtctggc ggacaatgag cagcttgcgc ccggtcagtt tttcatcctt
    25501 caccgttgag acggttgtgc cgatcactct ggcaataagc atcagttcct cctaataggc
    25561 cttcacctcg ggcgcggcag gctgcggttt ggtctcgctt tggtccgccc ggataatctt
    25621 caccccggcg ctggcgccga tgcgcgtggc tccagccgcc accagggctt cagcctcctc
    25681 gtgggtgtga atgccccctg aagccttgac ccccagcgat ggtccaactg tgcggcgcat
    25741 cagcgcgaca tcatgcacgg tggcgccccc gcccgagaac ccggttgagg tttttacata
    25801 atctgccccg gcttccttgg ccaacaggca ggcaatcacc ttttcttcat cggtgagcag
    25861 cgcggtttcg atgatcacct tcaccagcgc gttggccgcg tgcgccacct ccaccacacc
    25921 gcgtatatct ctggcagcca ggttggtatc gcctgctttt aacgcgccta tgttcaacac
    25981 catatcgatc tcggtcgcgc cattttccag cgcgttgcgg gtttcaaatg ccttgacctc
    26041 gggtgaggtc gcgcccagtg gaaagccgat gactgtgcac accttcacct cgctatcttt
    26101 tagcaggtca gcgcatagtt ttacatgcgt ggggttgaca cacacggagg cgaaatgatg
    26161 ctttttggct tcaaaacaca actgcgcgat cttgtcgctg gtggcatccg gtttaagcag
    26221 ggtatgatcg atcatccccg ccagcgcgct atccccaggg atgacaccga ccgtcgaggt
    26281 aaggcgttcg gcgccagcgc tgatcacatg cccggcatta tcaaaacagg ttttcacttt
    26341 taccccgttg gcgacctcca ccacgcaggt ggcgccagga ggattttttt gtctctcctc
    26401 ctgttcgatc atcgccagca gcacttcgcg ggtgatgatc tcaaccaggg tttcaacggt
    26461 cttgatatca ggtgtcatgc tgttttcccg ctaagatatt tcttctcgat ctccatgatc
    26521 ttatccacac gtctggcgtg cctgtcgccg ccgaactcag tggtcagcca ggttttgagc
    26581 acctgtttgg caaggttgac cccgatcaac ccggcgccaa gcgtgagcac gttggcgtca
    26641 ttatgctcgc ggctgttcac cgcggtagcg taatcatagc ataaagccgc tctcaccccc
    26701 ggcactttat tggccgccat gcaagaacca atgcccgcgc catcaatcac cacgccacgc
    26761 caggctgcgc cttgcgcaac ctgcagcgcc actgccaggg caatatccgg gtaatccaca
    26821 gggtcgctcg tgttcgtgcc gcaatccata attttaaaac ccatgtgtga aatttcctgg
    26881 atgagaacct tttttaactc cacgccgcca tgatcggttc caat
//
LOCUS       DAOSPM010000014        26152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1870_length_26152_cov_15.226961, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000014 DAOSPM010000000
VERSION     DAOSPM010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 26152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26152
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1870_length_26152_cov_15.226961"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLW25_02490"
     CDS             complement(<1..692)
                     /locus_tag="PLW25_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53388.1"
                     /translation="MTNNFRKKSILIVVLAVFLMACSLTGLLPGRDKDTASSGEYRSE
                     VGGYSFKPIEGYELIEFYGITQMMAPGGNDETGPMIAMMGAKQEEKLDNQKLLGNMKE
                     SLPDAKFSKPKKVKVDGKAGLDIKLEDYEDEDVMGRLVIVMVEPTQHFVLLGVAPKAE
                     WKALNKNYEKVLKSIKFFEPKEMSFDEDIYDDGYIVSPDDFDFEPGQFSTYDEDGQLH
                     QWAVSAVASSQYG"
     gene            929..1028
                     /gene="ffs"
                     /locus_tag="PLW25_02495"
     ncRNA           929..1028
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLW25_02495"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1054..1953
                     /locus_tag="PLW25_02500"
     CDS             1054..1953
                     /locus_tag="PLW25_02500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS53389.1"
                     /translation="MQTPPVCNYEGSDYQTSFWEKGGRDYEDGCEAVALKRLLPAAGS
                     RLLELGAGAGRNTSRYSGYAQVTLLDFSRTQLEQAKARLGTGERYRYIAADIYRLPFV
                     KGLFDGATMIRTLHHMAEPALALAQVRRVLEPEATFILEYANKRNLKSILRYLLGRQK
                     WSPYTREAVEYIPLNFDFHPAAIRDWMDGAGFILERALTVSHFRVSFLKKTLPVRLLV
                     AVDALLQPTGGLFQLTPSVFLRARALKTGVKAPQGAFFACPACAAPLDDTPPLIHCPA
                     CEKQYPVIDDIYDFRLNPPAEGD"
     gene            1957..>2570
                     /locus_tag="PLW25_02505"
     CDS             1957..>2570
                     /locus_tag="PLW25_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOS53390.1"
                     /translation="MPDALLDDLNPQQRRAVTAGDGAVLVLAGPGSGKTRVLTRRLAY
                     LVERRGVPACDVIAVTFTNKAAREMENRVLNLVQQNLDGIWLGTFHAMCARLLRREVN
                     YLPYRSNFVIFDADDQESLVKRAIRELNIDDKTHRPAGVHAAISAAKNDLLLPEQMPL
                     RTYRDEVVARVYKRYQEMLVANNALDFDDLLLQAARLLEQNPGVA"
     assembly_gap    2571..2670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2671..4340
                     /locus_tag="PLW25_02510"
     CDS             <2671..4340
                     /locus_tag="PLW25_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOS53391.1"
                     /translation="DDLLLQAARLLEQNPGVAERYARRFQHVLVDEFQDTNLAQYTLL
                     QQICAYHKNIFVVGDEDQSIYRWRGADYRNVLRFQQDFKDCATILLEQNYRSTQNVLD
                     AARQVIDRNGNRTPKHLFTERGTGSKLVQFTAVDEVAEAAFVVDTIQQYLSSMRATAS
                     DFAVMYRTNAQSRLVEEALIRARLPYRLVGALRFYGRREIKDLIAFLRLAENPADEIS
                     LARVIAVPPRGIGQKTIEMLQSAAFQANISSGEVLLDLGSKGDSSPFWAIIGRSASLL
                     ADFGALLADWHSLKGRVSLVTLFDRIISDTGYEPYINDQSDEGNDRWENIQELRRLAY
                     DYDEKGLTEFLQNLALVSDQDTLPPEADQPATARQKAVTLLTLHAAKGLEFNQVFITG
                     LDEGLLPHSRSRDDPEEMAEERRLFYVGMTRARDQLYLLRSERRSSFGNWEYSEPSRF
                     LADISDELLTYQGKRGGLPRAAQFEELRWTTTGLPSTYSRRQRKPELPVETRFKPGMR
                     VRTAAWGEGLVLESRVDSDGEETVDVHFESVGFKRLLASLAKLEIIP"
     gene            4439..5947
                     /locus_tag="PLW25_02515"
     CDS             4439..5947
                     /locus_tag="PLW25_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HOS53392.1"
                     /translation="MSPIVAALPILLVLVFMLFLRRGSHEAGFGGWLVGIAVAFLFFG
                     LNWNVLWVSQLKALLLSLNVLTVLWPALLMYHIVEMSGGVRAIAYALQEMIPEPGWLL
                     LVLAWMLSALVENLAGFGVPIAVIAPMLILLGVIPVRAVAAAAIGHSWTVSLGGMALA
                     FRTMTDITAMNESEVFPFAAVLLGVTVLLTGLGSAYVLKQLHYWKRILILSLVVALTQ
                     YFIGITGVIPLSSFSAAIVGIGGGLLLTRRPPGQKIRASTSPQLRGGLFSYGTLFGSI
                     FIISIIKPLNRWLAGARVSSWFPQVVTNQGFTTAAGPGFVFKPFTHPGVWILFSALVA
                     ALVLPRLTRSGTAHMKESLTATWRSALPASLGTLFMIGLSSLMEHSGMTQLIAQGLSQ
                     ALSSYYPLVAPLVGTLGAFATGSNTNSNILFGQMQKGVAALLGISPLVLLAAQTTGGA
                     LGSMISPAKLAVGGSTNAMKGKEGAALRLTLPIGLASTLFIGLLAWILLKVL"
     gene            complement(6072..7109)
                     /locus_tag="PLW25_02520"
     CDS             complement(6072..7109)
                     /locus_tag="PLW25_02520"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOS53393.1"
                     /translation="MITEKQVYDALSGVMDPELGRSLVELGMIRNVTISRGGVVAFTL
                     AFTIAGCPLKQKIERDAREAVMKLPGVKQVNITQSVMSEEERKALFPPGATELPQLNK
                     FNKIDRVIAVMSGKGGVGKSSITALLACELARQGKKIGILDADITGPSIPRLFGLPPG
                     GLRGSEQGILPAISGLGIRVMSSNLLVPTEEAAVIWRGPLIAGSIQQFWRDVLWGRLD
                     VLLVDLPPGTSDAALTVMQTLPVNGVVLVSTPQQLAGMVVRKADSMLKQLNIPVLGVV
                     ENMSYYPCPDGGGNHEIFGPSHVEELAAAVGAQRWLKLPLDPRVALLGDTGRIEEYRA
                     ENLQEILTLFS"
     gene            7181..7783
                     /locus_tag="PLW25_02525"
     CDS             7181..7783
                     /locus_tag="PLW25_02525"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOS53394.1"
                     /translation="MKRSLFLSLLFVVAAVLMACSASVDLFGNKVKPTLNIPPTQPPA
                     IATSSTPLQPTVIIALPPPPSLETSITVYFMDEDRFINGTEPYEIAVSRTTASVNLPL
                     AVLQAYFAGPTVDEYNQGLRMLGSGFSYVRDLTVENGIARVYLGGECANNGAAYSVAA
                     LIFKNLQQFPEITAIKIYDENNSTLDPDSAHSSLPYCLEP"
     gene            7874..8575
                     /locus_tag="PLW25_02530"
     CDS             7874..8575
                     /locus_tag="PLW25_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOS53395.1"
                     /translation="MTITILSVLFAVTLVFLALSRMAVERIETHHYLQRADFFQRYPV
                     QQGDIVFLGDSITDGGCWEELFPGVPLKNRGINADNTRGVLERLDDILRPQPRAIFLL
                     IGTNDLPWYTYLNDRAILHTYNQILDRCRALAPDAVVHVQSLLPRQRIYAARILRINA
                     HLETMASRHGYPFINLFPAFADRQGALRADLTNDHLHLMAEGYALWVSILKPYIEQLH
                     NENVNSALPSPGFNP"
     gene            8755..9459
                     /locus_tag="PLW25_02535"
     CDS             8755..9459
                     /locus_tag="PLW25_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOS53396.1"
                     /translation="MSHFTQKSLFWISKPVVKTYTNAMLKMDVHQKTPFVSGAKIIAP
                     NHPSTTDPFFVAAMLGQQSYIMIKDVLFNVPVLGEYLKRSGHIKVAAGQGQEAIDQAL
                     AHLAVGHTIMIFPEGKVSPVKGGFNQVRSGVARLAIASGAPVYPVGIHLLKERIHAIS
                     SRVKGRDEMGYWYLRGPYNISVGKPMRFNGDVDDHEFVRETARLVMLKIMRLAYESEQ
                     RWYRKTRAVVGSLETA"
     gene            9575..10612
                     /locus_tag="PLW25_02540"
     CDS             9575..10612
                     /locus_tag="PLW25_02540"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOS53397.1"
                     /translation="MKPQQILAGPGMLLDGQGNLIQAGWATQPVLDANLENVHFYAFR
                     PLQKLRLKLWDYYAITTPTHFFSFTVSDIGYLGMVFAYVIDFKTGAFKEETLALPFGG
                     GVKLPRNSTEGVTEYSKKDLYLRFRAEGEVRQLSIRWPGFGGSALNAEVALTCPADHE
                     SMNIVIPIPGKRFYYNRKVNCMPAEGWVEYQGTRHLIEPTTCLGSLDWGRGVWEYSSF
                     WVWASASGFLPDRRRIGLNMGCGFGDTSAATENCFILEGKVHKLSELKFTYDNRNFKA
                     PWTMSSPDGRLELVFTPFFERVAKIDALVLKSEVHQLIGRYNGKVITDTGEKLEIVDL
                     IGWAEEHNAKW"
     gene            10903..11778
                     /locus_tag="PLW25_02545"
     CDS             10903..11778
                     /locus_tag="PLW25_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS53398.1"
                     /translation="MKKLTIIMPVYNEEESLPLIMNNWLELCAASHCRLVVVNDGSTD
                     GSRRILDNLQSQHDFTVIHHKVNKGYGGAIKTGFRAVKTEYLVTIDGDGQHLPASVIH
                     MYQKMIEEDCDLLIGARENETKFTARGLGKWIIRSISKLLLPNKIRDLNSGLKMYRAE
                     LAQRYIKVCPDSMAFSDIIALTFIAEKCKVMETPIQISPRAGGKSKINLGSAVDTVIE
                     IINIVVFFNPLRVFLPLSAFFLVAGLGWGIPIVLKGRGVSTGSLLAFTISGIILLLGL
                     IAEQLSQIRRILIEE"
     gene            complement(11849..13564)
                     /locus_tag="PLW25_02550"
     CDS             complement(11849..13564)
                     /locus_tag="PLW25_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53399.1"
                     /translation="MPFTSGIKKCRLNEVFLLAEIIISAAVFILLGIFLAKYAGGPIY
                     SDEVMYINHGLLNVKDPFIINRYTHIFFQKLFVEIADRPLEGVKAYWGFLVAGSAALT
                     YVISRLTTKTSNILHAGISTLLYLTIPLLSVYSGNTSVDLTAGIFLGLTVVLYILCFR
                     QPGKPVYLLSLMGLAFFLAFRSKETTLASAVLLIGFLFDGEGGINVRLCLKRLGYFMI
                     GLLAGVVLFMLLNGLLLKDAWFGLRLSEYFQFKKTYIDRNIGEESKAVANWFTGFFLE
                     STPYIFLAYLFSGIKLLKKQPPSMRLPWLIPLKLIVILSVIIGFSSWGVIGRHVFPIL
                     PLVCAFAPQLIVFDFPRNKKEFSWLAFSMLAAALLFIAGRKILVVVSQAGPYNYPDFL
                     ASTVYPITLIALIILVFLVRHYSNKTVFAPLLVFVIFIAYPLYDNTLNYFFEQTNVRK
                     MNERLQAIEVFDSANIKEEKIKLLVFEDALSDLNYSSRDVEFIDLFNMYYDERLTWSD
                     VRVVNFDTFKPQTIVEWQPTFILMSADEYSSSEASVIIQRYIESDYEMIDSGNIRFAL
                     FNRLK"
     gene            complement(13765..14040)
                     /locus_tag="PLW25_02555"
     CDS             complement(13765..14040)
                     /locus_tag="PLW25_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53400.1"
                     /translation="MSSNLCPFPFLIPDFLCNAGGVTVSYFEGVQNDMNYYWTKEEVL
                     EKLDTKMTIAFAGVLDMSQKSKVYTRDAAYMVAIDRVVKAMQLRGWV"
     gene            14030..14284
                     /locus_tag="PLW25_02560"
     CDS             14030..14284
                     /locus_tag="PLW25_02560"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HOS53401.1"
                     /translation="MDDIIFTCDDQNLKSLYTMTLPDGRLALVFTPFYERVEKSDTLV
                     LKSGSQQMFGRCKVKRLTISGEKLDIIDLIGWVEEYNAKW"
     gene            complement(14295..16331)
                     /locus_tag="PLW25_02565"
     CDS             complement(14295..16331)
                     /locus_tag="PLW25_02565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOS53402.1"
                     /translation="MLIFSALVYLFLALLLSLRIFKSVSFSHSVIIFFLSSFTLNVLI
                     AEVLSLLGMLNSRWMYLLLQLLFCLALAMLIWDPPKKVFKNPIPKLKFEFRRLYGWEI
                     FLVALVSGILLLCLYIGALAPINNSDSLHTHLPRIYYWLQHGSLASWDAVTVTQLNYP
                     VNISIQGASLFLLGRSEKLFYLVPWLALLAVVLLIYEIAMLLGASRRAALFAGLLGLS
                     FPVVLLQTFSYQGDVLVATLVLAAVWFLLLYVRQQESFFLYLSFVPLAAALGSKQTAF
                     LFLPFFLLALLILFIKNHTRVEVILGAAGVFLAFFMLLASYKFIQNAVERDHLESSMF
                     AGYRYRLPFSKPGDGLHYATNSGRYLYQAVSLDGLTGRLKLAAQEARAHAFRAKSEWL
                     NLDLEVRDYISEGDDEYFSFAEAPALNEDSAWFGPLSFVLFPIAVVLVLTGKCKLCKR
                     YQWLSLVFMLTVFLMVAVLITGWSPTNGRYLILPMLVFTPLFAVLIPQKRAWSGLVTL
                     ALSIASFYLAISTLLINDARPLVTQQSLYSFQAKKVEKWGDSGFIGGIAAKHIANRVI
                     EDLVLTSPDRKDIRQQSYYENLFHQSTSAIADVEFVNSTVKPHEKLYLFIDKDIIEYA
                     LFGVNKTRDLVPVVSLQQLPDGALVLVDKRRIPSITSGFTLLAENDHFSIYQKP"
     gene            complement(16360..16755)
                     /locus_tag="PLW25_02570"
     CDS             complement(16360..16755)
                     /locus_tag="PLW25_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53403.1"
                     /translation="MLGEIHKENVANASPPLIAVFIPAYVSFFLIASLMLVFLGGIWG
                     TVKWLEPDLSEVFTTTGIVFIAVPSIIQAMQFLIQTITLDTSSVSVEPLVRRMPKKPS
                     MKGPLMMWLHERIERGGVRVPLSPVLHPA"
     gene            complement(16846..18132)
                     /locus_tag="PLW25_02575"
     CDS             complement(16846..18132)
                     /locus_tag="PLW25_02575"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOS53404.1"
                     /translation="MATSINAFEMAQKQFDNVAELLKLDPQVREILRWPLREFSFRIP
                     VRMDDGSLRMFQGFRVQHNDARGPNKGGIRFHPAETLDTVRALATWMTWKCAVADIPL
                     GGGKGGVVVDPSTLSTGEKERLVRGWVDVMWRNIGNRQDVPAPDVGTTAQMMGWMMDE
                     YSKLVGEFTPGVITGKPLGGGGSSGRTEATGFGVIINVREAMKHLKLDGAKCTASIQG
                     FGNVAQYASIGFTEMLKGKVICISYYDREDKVSYTVSKADGIDPRFLMTITDQYGTVD
                     KVKAREAGYVIEDGGAWISKDVDVLIPAALEGQINGETVKQISPRVKILAEGANGPTT
                     PEADEVLKQSDIFVIPDFLCNAGGVTVSYFEGVQNDMNYYWTKEEVLEKLDTKMTIAF
                     AGVLDMSQKSKVYTRDAAYMVAIDRVVKAMQLRGWV"
     gene            complement(18361..19857)
                     /gene="gatB"
                     /locus_tag="PLW25_02580"
     CDS             complement(18361..19857)
                     /gene="gatB"
                     /locus_tag="PLW25_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOS53405.1"
                     /translation="MDGTGCDKIHYIMQYEAVIGLEVHAELATQTKMFCGCRVVDTTT
                     SRPNSSVCPVCAGMPGTLPVLNRRAVEYGVRVALALECEVNHTSIFARKNYFYPDLPK
                     GYQISQYEQPLAENGRLVIDTSEGERVIRVRRAHLEEDTGKLTHITDDGGDCSLVDLN
                     RAGVPLLEIVSEPDMHSVEEVRAYATGLRAILRALGVNSGNMEKGVMRFEANVSIRPA
                     GSDQLGTRVEIKNLNSFRAMERAIAYELARQASLLENGETVSQETLGWDEPNQRTYVQ
                     RSKEEAHDYRYFPEPDIPPLVVEADWIAQIRQRMPELPRDKTRRLQQQYGLIPADAAI
                     LTSESDIAAYFEDCAAALKQASARSAANWITGELFAHLNSTGAEITHIRVSPQSLAEL
                     LDIIEAGGINQNTAKTVLAEMLASGSSAQQVISEKGLAQVTDTGFISDLVKEVLTTNQ
                     KELASYLAGKETLANWFFGQVMRAAGGKASPAVLKEELEKQLTEARRG"
     gene            19928..20698
                     /locus_tag="PLW25_02585"
     CDS             19928..20698
                     /locus_tag="PLW25_02585"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOS53406.1"
                     /translation="MLLCIDIGNTNIKLGLYEGEKLVTHWRIYTDEHKLADEYAVLLL
                     SLFKSEDIKNEAISGCAISSVVPELTIAFRDLVRRHLNLEPVIVGNVKRPVIHINTSN
                     PKEVGPDLIANAVGAVALYGAPCIVIGFGTATTFCAISASGDLEGVVIAPGVITAASS
                     LFQAGAMLPAVDLHKPEKVIGKNTILSLQSGLIYGFAGLVEGLSARIKAELGGDAKVV
                     ATGGLARLMADEVDCIDAVEPELTLLGVRIIYEKNQKG"
     gene            complement(20975..22135)
                     /locus_tag="PLW25_02590"
     CDS             complement(20975..22135)
                     /locus_tag="PLW25_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS53407.1"
                     /translation="MTDRYLSRRVAGLKPSGIRKFFDITATMKDVISLGIGEPDFTTP
                     EPILMAGIESLQRGETHYTSNYGLIQLRRALAAHFSTLYNVEYDPEKEIIVTVGGSEA
                     LNLAAIALVNPGDEVIIPTPCFVSYQAAVILAGGVAVEVPSHMESNFDIDPDALRAAI
                     TPRTKAILIGFPNNPTGAVASRENLLEVARIAEEHDLVVISDEIYDRLVYGVKHVCFT
                     ALPGMRERSLLLGGFSKDYAMTGWRIGYAAGPEHLITGLLRVHQYMVMSAPTMSQFAA
                     LEGLLSGEPHVLKMLAEYDRRRKLIVSGLNQIGLPTFEPKGAFYAFPKVSVTGLDDDT
                     FCTRLLHEQRVAMVPGNAFGMGGEGFARCSYATGYNQLEEALERIERFVRKI"
     assembly_gap    22228..22327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22328..22523)
                     /locus_tag="PLW25_02595"
     CDS             complement(<22328..22523)
                     /locus_tag="PLW25_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53408.1"
                     /translation="MRCRAEYAYPGRPLEVWDGRRWQKVIEVLDEIQTPLDKRFKVVC
                     ENGNEYWLVYDPDRDGWQVTA"
     gene            complement(22532..23545)
                     /locus_tag="PLW25_02600"
     CDS             complement(22532..23545)
                     /locus_tag="PLW25_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOS53409.1"
                     /translation="MSQVFKIIIPMAGYGSRMRPLTWSRPKPLLPLAGKTVLDYALEQ
                     FSSLPGVENAEYVFITSPNQGDAIEQYMRHAHPQKKVHFVIQAEMRGQSHALWCAKEY
                     LAGPALIAFSDTLIETDLQFIPFEEMDAVAFVKAVEDPRRFGVIEIGKNGIATRLVEK
                     PQDTSNNQVLVGFYYFKDTAALLRAIEEQMRLGKSLKGEYFLADAINIFIQMGGRMRK
                     ENIDTWLDAGIPSAVLETNRHLLDQGRVATQPDSGIPGATIVPPVFIAPGAKIEACVI
                     GPYVSVGKGCTLKNVVINNSIVDDNTVIENMVIEGSIIGREVVYKGQPARLFVGDHTQ
                     VQS"
     gene            complement(23710..24150)
                     /locus_tag="PLW25_02605"
     CDS             complement(23710..24150)
                     /locus_tag="PLW25_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOS53410.1"
                     /translation="MTQQNTRVELIERGPGCLVRGLYFLFIGLWLGLIWVVVAWFFNL
                     TIVGLPIGLLMINRIPQVMTLRPQRVQTTVEIREGSPVIRQTTIRQNPFLLRALYFLV
                     IGFWFSLVWMLLAWLLTAVTFGLAFPLTFWMFDQVPAITTLARY"
     gene            complement(24243..25721)
                     /gene="gatA"
                     /locus_tag="PLW25_02610"
     CDS             complement(24243..25721)
                     /gene="gatA"
                     /locus_tag="PLW25_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOS53411.1"
                     /translation="MNTSLTDLSVIQILDGLKKAEFSCRELVQAYLERIAILEPRVHA
                     FVTLAEEDALSLADKADARYASARHSGSETPPLLGLPIAVKDLITVAGMRCCCGSAIL
                     KDFVPPFTASAVQRLLDAGAIVVGKTNTDEFAMGSSTETSAFGPTFNPWDVTRVPGGS
                     SGGSAAAVAAGMVPAALGSDTGGSIRQPASFCGVCGIKPTYGRVSRYGLVAYGSSLDS
                     MGVFTRNAEDAALLFSLMAGIDPLDSTTVDVPVPVLNLREKERLDGLRVGIPQEYFIE
                     GIQPQVRQCVLAAIDILRLLGAELVEVSLPHTEYALPVYYLIAPAEASANLARYDSVR
                     FGLREPAATLWEMFKRSRGVGFGAEVKRRIMLGTYALSAGYYDAYYGQAQKVRTLIRR
                     DFDQAFEHVDLIACPVAPSTAFKLGEHAGDPLAMYLEDVFTLPTNLAGIPGLALPVGF
                     DDNHLPIGIQFLGAHFREDVLFRAAHAYQGVTDWHQQVPALD"
     gene            complement(25718..26005)
                     /gene="gatC"
                     /locus_tag="PLW25_02615"
     CDS             complement(25718..26005)
                     /gene="gatC"
                     /locus_tag="PLW25_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOS53412.1"
                     /translation="MKLSLVEVEHIAELARLDLTAEEKEQYRQQLSAILEHVARLQAL
                     DTEGVPPTSSVLPANGRLRVDEPRPGLTVESVLANAPEKMKDQFRVPPVLE"
BASE COUNT         6078 a         7113 c         6776 g         5985 t
ORIGIN      
        1 ccgtactgtg aactggctac cgcgctcacc gcccactgat gcagttggcc atcctcatca
       61 taggtggaga actgaccggg ttcaaagtca aagtcatctg gcgagacgat atacccatca
      121 tcgtagatgt cttcgtcaaa actcatttcc ttaggttcaa agaatttaat tgactttagc
      181 accttttcat aatttttgtt gagcgctttc cattctgcct ttggcgcaac cccaagcagg
      241 acaaaatgtt gcgtcggttc aaccatcaca atcaccaagc gccccataac atcttcatct
      301 tcgtaatctt caagcttaat atccaggcca gccttgccat ccactttcac cttttttggc
      361 tttgaaaatt ttgcgtctgg taaagattct ttcatgttcc ccagaagctt ctggttatcc
      421 aatttttctt cttgttttgc ccccatcatg gcgatcatcg gtcctgtttc atcgttccca
      481 cctggggcca tcatctgggt gatgccataa aactcgatca gttcgtagcc ttcaatgggc
      541 ttgaatgagt acccgccgac ttcagagcga tattcgcctg aagacgccgt gtccttgtct
      601 cgccctggca acagcccggt caggctgcag gccattagaa agaccgccag aaccacgatt
      661 aaaatgctct tttttctgaa gttatttgtc atgctctcct ccttgtgatt attctatttc
      721 aattggtgtt tgctggcagc aggtatctcc gcctgactac ccaagagcaa ctttgataga
      781 attatagcag aacctaactt tttcctgcct tcattttcgc aaacctcccg catcgcttat
      841 tgaacgcaaa ggagatcaac tcttaaggat tggattgata atccgttgac gcagggcgct
      901 ggatagggta tccttaaccc cgcgctccgg atgggaccgg cgcggcagag ccctgcgaac
      961 cccgtcaggt ccgagaggaa gcagcggtaa ggaggatacc ctgggcgccg ccggcaaacc
     1021 tgtccggacg cacttttcac actctggaga atgatgcaga cacccccagt atgcaattat
     1081 gaaggctccg attaccagac cagtttctgg gaaaaaggtg ggcgcgatta tgaagatggc
     1141 tgtgaagccg tggcgctcaa gcgcttgctg ccagccgccg gtagccgctt gctggagctg
     1201 ggcgcgggtg ccggtcgtaa cacctcgcgc tacagtggtt acgcccaggt gaccctgctg
     1261 gatttcagcc gtactcaact tgaacaagct aaagccaggt tgggaacagg tgaacgctac
     1321 cgctacatcg ccgcggatat ttaccgcctg ccgttcgtga agggactgtt cgatggcgcc
     1381 accatgattc gcacgctgca tcacatggct gaacctgcct tggcgctcgc gcaagtgcgc
     1441 cgcgtgcttg agcctgaagc cacgttcatc ctggaatacg ccaataaacg caacctcaaa
     1501 tcgatactgc gttatctgct ggggcggcaa aaatggtcgc cttatacgcg agaggcggtg
     1561 gaatacatcc cgctcaattt tgattttcac cccgccgcca tccgggattg gatggatggc
     1621 gcgggattca tcctcgaacg cgctcttact gtcagccact ttcgtgtgag ttttctcaaa
     1681 aaaaccctgc ccgtccggct gctggtggca gtagacgcct tgctgcagcc taccgggggg
     1741 ctgttccaat tgactccgag tgtgtttttg cgcgcacggg ctttaaaaac gggcgtaaaa
     1801 gcgccacaag gcgcattctt cgcctgcccg gcctgtgccg ctccattgga cgataccccc
     1861 ccattgattc actgccccgc ctgtgagaag caataccccg tgatcgatga tatttacgac
     1921 tttaggctca acccgccggc ggagggggat taactcatgc ctgatgcgct gctggatgac
     1981 ctgaatccac agcaacgccg cgcggtaacc gcgggtgacg gcgctgtgct ggtgctggct
     2041 ggaccaggct ccggcaagac gcgcgtgctc acgcgccggc tggcttacct ggttgagcgc
     2101 cgcggcgtgc ccgcttgtga tgtcattgcc gtcaccttta ccaataaggc agcgcgcgag
     2161 atggaaaacc gtgtgctcaa cctggtacag caaaaccttg atggcatctg gctgggcacc
     2221 tttcacgcca tgtgcgctcg tctgctgcgc cgcgaggtca attacctgcc ttacaggtcc
     2281 aacttcgtca ttttcgatgc tgatgaccag gagtcgctgg tcaagcgcgc gatcagggaa
     2341 ttaaatatcg acgataaaac gcatcgcccg gcaggcgttc acgctgccat ctccgccgcc
     2401 aagaacgacc tgctgctgcc ggaacaaatg cccctgcgca cctaccgcga tgaggtggtg
     2461 gcgcgtgtgt acaagcgcta ccaggaaatg ctggtcgcca ataacgcgct ggatttcgat
     2521 gacctgctgc tgcaggcggc tcgcctgttg gagcaaaacc ccggcgtggc nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcgatgacct gctgctgcag gcggctcgcc
     2701 tgttggagca aaaccccggc gtggcagagc gttacgcgcg gcgcttccag catgtgctgg
     2761 tcgacgagtt ccaggatacc aacctggcac agtacaccct gctgcagcaa atatgcgctt
     2821 atcacaaaaa catctttgtg gtgggcgatg aagaccagtc catctatcgc tggcgcggcg
     2881 cagattaccg caatgtgttg cgttttcagc aagattttaa agattgcgcg accatcctgt
     2941 tggagcagaa ctaccgctcc acccaaaatg tgcttgatgc cgcgcgccag gtgatcgacc
     3001 gtaacggcaa ccgcaccccc aaacaccttt tcaccgagcg cggcacgggc agcaagctgg
     3061 tgcagttcac cgcggttgac gaggtggctg aagccgcctt cgtggtggat actattcagc
     3121 aatacttatc ctccatgcgg gcaaccgcca gtgacttcgc tgtgatgtac cgcaccaacg
     3181 cccagtcgcg cctggtggaa gaagccttga ttcgcgcccg cctgccttat cggctggtgg
     3241 gggcactgcg attctacggg cgccgcgaaa tcaaagattt gatcgccttc ctgcgcctgg
     3301 cagagaaccc cgctgacgaa atcagcctgg cgcgcgtgat cgccgtccct cccaggggaa
     3361 tcggtcaaaa gaccatcgag atgctgcaaa gcgccgcctt ccaggccaac atcagctctg
     3421 gtgaggtact gctcgacctg gggagcaaag gagactcttc tcccttctgg gctatcatcg
     3481 ggcgctcagc ctctttattg gcggattttg gcgcgcttct cgcggattgg cactctctta
     3541 aagggcgggt ctcgctggtc actctctttg accgcattat cagtgacaca ggttacgagc
     3601 cttatatcaa cgaccagtcg gatgaaggca atgaccgctg ggaaaacata caagagctgc
     3661 gccgcctggc ctatgattat gacgaaaaag ggttgactga atttttacaa aatttagccc
     3721 tggtctcaga tcaggatacg ctgccgccag aagctgacca accagccacg gcacgccaaa
     3781 aagcggtgac gctgctcacc ctgcacgctg ccaaagggtt ggagttcaac caggtgttca
     3841 tcacaggttt ggatgaaggg ctgctaccac acagccgcag ccgtgatgac cccgaggaaa
     3901 tggcagagga gcggcgcctg ttctatgtgg gtatgacacg cgcccgtgac cagctgtacc
     3961 tgttgcgttc tgagcgccgt tccagttttg gcaattggga gtacagcgaa ccatctcgct
     4021 ttttggctga catcagcgat gagctgttaa cctaccaggg caaacgcggg ggtttgccgc
     4081 gtgcggcgca atttgaggag ttgcgttgga caacaactgg cctgccttca acttattcca
     4141 ggcggcaaag aaagccagaa cttcctgtgg aaacgcgttt caaacccggc atgcgcgtgc
     4201 gcaccgccgc ctggggtgag gggctggtgc tcgaaagccg cgtcgattcc gatggggaag
     4261 agacagtgga tgtgcatttt gaatccgtag gttttaagcg cctgctggct tcgctggcga
     4321 aactggaaat aattccatga cgaatattga cacgtgttgg aacctggggg gatagaaatt
     4381 acttaccttc gttccatgag attcgatcgc actcattaca agcctggata attgaccaat
     4441 gtcacccatt gttgctgcgc tccctatttt actggttctc gtgttcatgc tcttccttcg
     4501 ccgcggcagc cacgaggcgg gctttggcgg ctggctggta ggcatcgcgg tagccttctt
     4561 gttttttggg ctcaactgga acgtactgtg ggtttcgcag ctgaaggctt tgctgctctc
     4621 actgaacgtg ctcaccgtgt tgtggcccgc cctgctgatg taccacatag tcgaaatgtc
     4681 cggaggggtg cgcgccatcg cctatgccct gcaagagatg atccctgaac ccggctggct
     4741 gctgttggtg ctggcgtgga tgctttccgc tctggtggaa aacctggctg gctttggggt
     4801 gcctatcgcc gtcatcgccc ctatgcttat tctgctgggg gtcatcccgg tgcgcgcggt
     4861 ggctgccgct gcaatcggcc atagctggac ggtgagcctt ggcggtatgg cgttggcctt
     4921 ccgcaccatg acggatataa ccgccatgaa tgaaagcgag gttttcccct ttgcggctgt
     4981 gttgctgggg gtgacggtgc tgctcaccgg gttgggttcg gcgtatgtgc taaaacaact
     5041 gcattactgg aagcgcatct taatattgtc gctggtggta gcgcttacgc aatattttat
     5101 cggtatcacc ggggtcatcc cacttagttc ttttagcgcc gccattgtgg gtatcggcgg
     5161 aggattgttg ctcacgcggc gcccgccggg gcagaaaatc cgcgccagta ccagcccaca
     5221 attacggggt gggctgttct cctacggcac cctctttggt agtatcttca tcatctccat
     5281 catcaaaccg ctcaaccgat ggctggcggg cgcacgggtg agttcctggt ttccgcaggt
     5341 ggtcaccaac caagggttca ccactgctgc cggtcccggg ttcgttttca aaccttttac
     5401 ccatcctggg gtgtggattt tattcagcgc gctggtagca gcgcttgtct tgccgcgcct
     5461 gactcgttca ggcaccgccc atatgaagga gtctttgacc gccacctggc gctcagcgct
     5521 gccggccagc ctgggcaccc tctttatgat cggtctatca tcgttgatgg agcacagcgg
     5581 tatgacccag ctcatcgcac aggggctcag ccaggcgctc agcagttact acccgctggt
     5641 ggcaccgctg gtgggcacgc tgggcgcctt cgccacaggc agcaatacca attccaacat
     5701 cctcttcggg cagatgcaga agggtgtggc agcgctgttg ggcatcagcc ctctcgttct
     5761 gctggcggcg cagaccaccg gcggcgcttt gggcagcatg atctcccccg caaaattggc
     5821 cgtgggcggc agcaccaacg ccatgaaagg gaaagaaggc gctgcgttgc gtcttacttt
     5881 acccatcggc ctggcaagca cgctgttcat cggcttgctt gcatggatcc tgttaaaggt
     5941 attgtaagaa ggccccaggg tctgtccatc tcgtaaattg gctgcctgta aaggggaacg
     6001 accgggcgtt tcgtgcaagc ggtttacaaa ccatcccgtt ctcgcgccca atccacccag
     6061 gcgtgggagt ttcaactgaa cagagtgaga atctcttgca ggttctccgc gcggtattct
     6121 tcgatgcgcc cggtgtcacc cagcagcgcc acgcgcgggt cgagcggcag cttgagccag
     6181 cgctgcgctc ccaccgccgc cgccagttct tccacgtggc tgggtccgaa aatctcgtga
     6241 ttgcccccgc catccgggca ggggtagtag ctcatgttct ccaccacccc cagcacggga
     6301 atgttgagct gtttgagcat ggaatccgcc ttgcgcacca ccatgcccgc cagctgctga
     6361 ggtgtagaaa ccagcaccac cccgttcact ggcaaggtct gcatcaccgt cagcgccgcg
     6421 tcagaggtac cgggcggcag gtccaccagc agcacgtcca gcctgcccca cagcacgtcg
     6481 cgccagaact gctgaatcga acccgcgatg agcggcccgc gccagataac ggcggcttct
     6541 tcggtgggca ccaacaggtt ggaagacatg accctgatgc ccagcccgct gattgccggc
     6601 aggataccct gctcgctgcc gcgcaacccg cctggcggta acccaaagag cctgggaatg
     6661 ctgggcccgg taatatcagc atccaaaatg ccgatctttt tgccctgtcg cgccagctcg
     6721 caagccagta aggctgtaat ggaggattta ccaacccctc ctttaccgct catcacggcg
     6781 atgacgcgat cgattttatt gaacttgttc aattgcggta actccgtcgc tcccggaggg
     6841 aagagcgctt tgcgctcctc ttcgctcatc accgactggg tgatgttcac ctgcttcacg
     6901 cccggcagct tcatcacggc ttcgcgtgcg tcgcgctcga tcttttgttt taacgggcag
     6961 ccggcaatcg tgaaggccag ggtaaaggcc accaccccgc cccgcgaaat ggtcacattc
     7021 ctgatcatgc ccagttccac caggctgcgc cccagttctg gatccatcac ccctgataaa
     7081 gcgtcatata cttgtttctc agtgatcata ttgctccaat acacaccatt cattatctgc
     7141 tggggatgat tataatggat acaaagaagg aggttagtca atgaaacgct cgcttttctt
     7201 atcgctgctc ttcgtggttg ctgctgtatt aatggcctgc agcgcgtcgg ttgacctgtt
     7261 cggaaacaag gtcaaaccaa ccctgaacat ccctcctacc caaccgcccg ccattgccac
     7321 ctcctccaca cctctgcagc ccacagtgat catcgcgctg cctcctccac cgtcgctcga
     7381 aacatcaatc actgtttact ttatggatga ggaccgtttt atcaatggca ccgaacccta
     7441 cgaaattgcg gtctcacgca ccaccgccag cgtcaacctt ccattggcag tgctgcaggc
     7501 ttacttcgcc ggtccgactg tggatgaata caaccagggt ctgcgtatgc ttggcagcgg
     7561 cttttcttat gtgcgtgacc tgactgtcga gaacggcatc gcgcgcgtgt acttgggcgg
     7621 cgagtgcgcc aacaacggcg cggcttactc tgtggcggcg ttgatcttca agaacctgca
     7681 gcaattcccc gagatcaccg cgatcaagat ttatgacgag aacaactcaa ctcttgaccc
     7741 cgattccgct cacagttcgc tgccttattg cctggagccg taaacccgtt tctatcataa
     7801 aaatgtgtca gccgcggctg gcagccggtt tttgcgactc aaccgccagt acgtaattgg
     7861 tataatcctg ctgatgacca tcaccatcct atccgttctg ttcgccgtca ccctggtttt
     7921 tttagcgctt agccgcatgg ctgtggaacg cattgaaact caccattatc tgcagcgggc
     7981 ggatttcttt caacgttacc cggtgcagca gggcgacatt gtctttttgg gggacagtat
     8041 caccgacggt ggctgctggg aggagttgtt cccgggcgtg ccgctcaaga atcgcggcat
     8101 caacgcggat aacacgcgcg gcgttctgga gcgcctggat gacatcttgc gcccccaacc
     8161 ccgcgccatc tttttgctca tcggcaccaa tgacctgccc tggtacacct accttaatga
     8221 ccgggcgatt cttcacactt acaaccagat cctcgaccgt tgccgcgccc tggctcctga
     8281 tgcggttgtg cacgtgcaat ctttactgcc aaggcaaaga atttacgccg ccaggatact
     8341 gcgcatcaat gcccatcttg aaaccatggc ttcacgacac ggctacccct tcattaacct
     8401 tttcccggct tttgctgacc ggcaaggcgc gcttcgcgct gacctgacca acgaccacct
     8461 gcacttgatg gcagaaggtt atgcgctctg ggtaagtatc ctcaaaccct atatcgaaca
     8521 attgcataac gaaaatgtaa actcagcatt acccagccct gggtttaacc cgtaaccgag
     8581 ccaatcaaac tgggtttaac tggttacgtt cctggtcata ttcattcgca tcatcgccgc
     8641 cgcaaaaata tgccctggtg caccggcagg aatatgcctt ttgggcggta cgcctggcat
     8701 aacaggatgg tattctttga cggtatcctg atctattaaa ggtaggttta ttctatgtct
     8761 catttcaccc aaaaatcgct cttctggatc tcgaagccag tggtcaagac ttacactaac
     8821 gccatgctca agatggatgt gcaccaaaaa acgccctttg tctccggtgc caagatcatc
     8881 gcgcctaacc atcccagcac cacagatcct ttctttgtcg ccgccatgtt ggggcagcaa
     8941 tcttacatta tgattaaaga cgtgctcttc aacgtgcctg tgttaggaga gtatctcaag
     9001 cgctcaggtc acatcaaagt ggctgccggg caagggcagg aagccatcga ccaggcgctg
     9061 gcgcacctgg cggtgggtca taccatcatg atctttcctg aaggcaaggt cagcccggtg
     9121 aagggtggct tcaatcaggt tcgttcaggc gtcgcccggc tggcgattgc cagcggcgcg
     9181 ccggtttacc cggttgggat tcacctgctc aaggaacgca tccacgcgat ttcatccaga
     9241 gtgaaaggtc gggatgaaat gggttactgg tacctgcgcg ggccatacaa tatcagcgtg
     9301 ggcaaaccca tgcgttttaa tggggatgtg gacgatcacg aatttgtgcg cgaaaccgcc
     9361 cgactggtga tgcttaaaat tatgcgcctg gcttacgaaa gcgagcaacg ctggtaccgt
     9421 aaaactagag cagtggtggg ttcgctggaa accgcctgaa cccgtaacaa ccataaagaa
     9481 aaaccgcgcc tttttttgcg cggtttttca tttgtcagag caaggtatat gagtacaata
     9541 acattagatc aatcaacagc aagcaggaga gaaaatgaaa ccccaacaaa tcctcgccgg
     9601 cccgggcatg cttttagatg gacagggaaa cctcatacag gcaggttggg ctacccaacc
     9661 agttttggat gccaacctgg agaacgttca tttttacgca ttccgcccgc tgcaaaaatt
     9721 acggctgaag ctttgggatt attacgccat caccacgccc acccatttct tctcttttac
     9781 cgtcagcgat attggttacc tgggcatggt ctttgcctac gtgatcgatt ttaaaaccgg
     9841 cgccttcaaa gaagaaaccc tggcgttgcc cttcggcggc ggggtcaagt tacctcgcaa
     9901 cagcacggaa ggggtgactg aatacagtaa gaaggacctg taccttcgtt ttcgggctga
     9961 aggagaagtg cggcagcttt caattcgttg gccaggcttt ggcggcagcg cgctcaacgc
    10021 tgaagtggcg ctcacctgtc cggctgacca tgaatcaatg aacattgtta tccccatccc
    10081 tggtaagcgc ttctactata accgcaaggt caactgcatg ccggctgaag gctgggtgga
    10141 gtaccagggg acacgccatc tcattgaacc aaccacctgc ctgggcagcc tggattgggg
    10201 gcgcggtgtg tgggagtaca gctctttttg ggtgtgggcc agcgcttcag gttttttgcc
    10261 tgacaggcgg cgcatcgggc tcaatatggg ttgcggcttc ggagatacca gcgccgccac
    10321 ggaaaattgc ttcatcctgg agggcaaggt gcataaacta agcgagctca aattcaccta
    10381 tgataaccgc aacttcaaag cgccatggac catgagctct ccggacggca ggcttgagct
    10441 ggtatttacg cccttctttg aacgtgttgc aaaaatcgac gccctggtat taaagagcga
    10501 agtgcatcaa ttgatcgggc gttataacgg aaaggtgatc actgacacgg gtgagaaact
    10561 ggaaatcgtc gacctgatcg gctgggctga agagcataac gccaaatggt gatttgttaa
    10621 aatgatggtc tttgaaaaat ggaaaagtga gcgttgtctg ctctctttgt gaagccgttg
    10681 gtgcacgcca acggcgttcg accctgatct accagcacag tccggcagag ggcgcttaca
    10741 gcgctggcag gcggttgggc ttcttctccc gagcgcgcaa agtgaaatat caagtgggtt
    10801 ttttattgta aggtgagaac agaaaaggtg atttcaggtc tctctgaaca tagtgcccaa
    10861 ttcatttata attagttcgt tcatgtttgt tcaaggagaa aattgaaaaa actcaccatc
    10921 atcatgccgg tttataacga agaagagtca ctgccgttga tcatgaacaa ctggctggaa
    10981 ctgtgtgccg catctcattg caggctggtg gtggttaacg atggctcaac cgatgggagc
    11041 cggcggatcc tggataacct tcaatctcag catgatttta ctgtcattca tcacaaggtc
    11101 aataaagggt acggcggtgc cattaaaacc ggcttcaggg cggtgaagac agagtacctc
    11161 gtcacgattg atggagatgg acagcatctt cccgcaagcg tgatccatat gtatcagaag
    11221 atgattgaag aagattgcga ccttctgatt ggcgcccgtg aaaacgaaac gaagttcacc
    11281 gctcgtggac tgggaaaatg gatcattcgc agtatatcta aactgctttt gccgaacaaa
    11341 attcgagatt tgaactcagg gttgaagatg taccgtgctg aattggcgca gcgttacatc
    11401 aaagtctgcc ccgattcaat ggctttcagt gacattatcg ccctcacttt tatcgcggaa
    11461 aaatgtaaag tgatggaaac ccccattcag ataagccccc gcgcgggcgg taaaagtaag
    11521 ataaaccttg ggtcagcagt ggacacggta atagagatta tcaacattgt tgttttcttc
    11581 aaccccttgc gtgtttttct ccccctctct gctttcttcc tcgttgcggg gttagggtgg
    11641 ggtataccga ttgtgttaaa agggagaggt gtaagcactg gctctttgct ggcctttacc
    11701 atcagcggga taattctcct tttaggctta atcgccgagc aattgtcaca gattcgcaga
    11761 atactgatag aggaatgaac cttctttaat cagcccgttc cgccagcgat cacctcccgc
    11821 tggcgctgcg gtgtgttttt cctggaaatt actttaatcg attaaaaagg gcaaaacgga
    11881 tgttcccgct atcaatcatt tcatagtcgc tctcgatgta acgctgaatg atcacggacg
    11941 cttcacttga agaatactca tccgcgctca ttaggatgaa agtgggttgc cattcaacaa
    12001 tcgtttgcgg tttgaatgta tcaaaattca caacgcgcac atctgaccag gttaaccgtt
    12061 catcataata catgttgaac aggtcaatga actcaacatc acgactgctg tagttaaggt
    12121 cgctgagcgc atcctcaaag accaagagct ttattttttc ctctttaata ttggcgctat
    12181 cgaatacttc aattgcctgt aatcgctcat tcatttttcg cacattggtc tgctcgaaga
    12241 aataattgag agtgttgtca tataacgggt atgcgataaa aataacaaag accaacaaag
    12301 gggcaaagac cgttttattc gaatagtgtc gaacgagaaa aaccaggatg attaaggcaa
    12361 ttaacgtaat cgggtaaacg gtcgacgcga gaaaatcagg ataattatag ggaccagcct
    12421 gactcaccac tacaaggatt ttccttcctg ctataaataa cagggcagcg gcgagcattg
    12481 agaatgccag ccaggagaat tcttttttgt tcctaggaaa gtcaaaaaca atcagttgcg
    12541 gggcaaaggc gcacaccagg ggcagaatgg gaaaaacatg ccgccctatc actccccagg
    12601 agctgaagcc aattatcacg cttaaaatta caatcaactt gagcggaata agccagggaa
    12661 gccgcattga cggaggttgt ttctttagca atttgatccc agaaaaaagg taggctaaaa
    12721 aaatgtaagg ggtgctttcc aaaaaaaagc cagtgaacca gttggcaaca gctttcgact
    12781 cctcgccaat attgcggtcg atgtaggttt ttttaaattg aaagtactcg ctaagtcgaa
    12841 gaccaaacca ggcgtccttt aacaaaaggc cgtttaagag catgaacaac acaacccctg
    12901 caagcagacc aatcataaag taacccaacc gttttaagca gagcctgacg tttataccgc
    12961 cttctccatc gaacaagaac ccaatcaaca aaacggctga agcaagggtg gtctcttttg
    13021 agcgaaaagc cagaaagaaa gccagcccca ttaaagaaag aaggtaaaca ggtttaccag
    13081 gctgcctgaa gcacagaatg taaagcacca cggtcaaccc aaggaatata cccgcggtaa
    13141 ggtcaaccga ggtatttccg gaatacaccg agaggagcgg tatggtgaga tacaacaaag
    13201 tcgaaatacc ggcgtgcaag atgttgctgg ttttcgtggt cagccttgaa atcacatagg
    13261 tcaaggctgc cgaacccgcc actaaaaaac cccagtaggc ctttacccct tccaagggtc
    13321 gatcagcgat ctctacaaaa agtttctgaa aaaagatgtg cgtatagcgg ttaataatga
    13381 atgggtcttt gacgttcagc aagccatgat taatgtacat gacttcatca gaatagatcg
    13441 gtccaccagc gtatttggcg aggaagatac ccagtaaaat aaaaaccgcg gcgctgatga
    13501 tgatttctgc cagtaaaaaa acctcattta atcgacattt ttttattccg ctcgtgaatg
    13561 gcatgtgttg ttcaaaaatc ctttcagggt ttatctcaat ttaaaaaccc gccgaagcag
    13621 ctgggagaac gaccttgcgg ttagattata ccttctgttt cagcggacaa aaaacccatc
    13681 caaccccctt tgtgagattg gatggatcag cgagtaatac ggttcgataa caagaattgg
    13741 ctttcttaat taccctttcg ctttttacac ccaacctctt aactgcatgg ccttgacgac
    13801 gcggtcgata gccaccatgt aagcggcgtc gcgggtatat accttgcttt tctggctcat
    13861 gtccagcaca ccagcaaagg cgattgtcat cttggtatcc aacttctcaa gtacttcttc
    13921 tttggtccag tagtagttca tatcattctg taccccttca aagtaagaaa cggtcacacc
    13981 accagcgttg cacagaaagt ccgggattaa aaatggaaaa ggacacaaat tggatgacat
    14041 aatattcact tgtgatgatc agaacctcaa atccctctat acaatgactt tgcccgacgg
    14101 tagactcgcc ctggtgttta cgcctttcta tgagcgcgtg gaaaaatctg acacactggt
    14161 attgaagagc gggagtcagc agatgttcgg gcgttgcaag gtgaaaagat taacaatatc
    14221 aggtgaaaag ctggatatta tcgacttgat cggttgggtt gaggagtaca acgccaaatg
    14281 gtaatctgtt cgaattacgg tttttgataa atcgaaaaat gatcgttttc cgccaggagc
    14341 gtaaacccgc tggtgattga agggattcgc ctcttatcca ccagtaccag ggcaccatcc
    14401 ggcagctgct gcagcgaaac gacgggtacc aggtcgcggg tcttgttcac gccgaacaac
    14461 gcgtactcga tgatgtcctt gtctataaaa aggtataatt tttcatgcgg tttcacagta
    14521 ctgttgacaa actcgacatc cgcaatggca ctggtgcttt gatggaacag gttttcgtaa
    14581 taagactgct gcctgatatc cttacggtcg gggctggtaa gcaccaggtc ttcgatcacc
    14641 cggttggcga tgtgcttggc ggcaatccca ccgataaaac ctgaatcgcc ccacttctcc
    14701 acctttttcg cctggaaaga gtatagcgat tgctgcgtaa ccaaaggccg ggcatcattg
    14761 ataagcaagg ttgatattgc gaggtagaag gaggcgatcg agagcgccag cgtcacaagt
    14821 ccgctccagg ctcgcttttg gggaatgagt acagcgaata gcggagtaaa taccaacatc
    14881 ggcaggataa ggtaacggcc gtttgtaggg ctccagccgg tgatgagcac cgctaccatc
    14941 aggaaaacgg tgagcataaa aacgagggat aaccactgat agcgtttgca caacttgcat
    15001 ttaccggtta aaaccaagac caccgcgata ggaaaaagca caaaagacag ggggccaaac
    15061 caggcggagt cctcgttcaa tgcgggagct tccgcaaaag aaaagtactc atcgtcgcct
    15121 tcagagatat aatcacgcac ctcaagatcg aggttgagcc attcactctt tgcgcgaaac
    15181 gcgtgcgctc tcgcttcctg ggcggcgagt tttaagcgcc ccgtcaaccc gtccaggctg
    15241 accgcctgat agagatacct gccgctattt gtggcgtagt gcaacccgtc acctggcttc
    15301 gagaacggta gccggtaacg gtagccagca aacatggaag attccaagtg gtcacgctca
    15361 accgcgttct ggatgaactt gtatgaggcc agcaacataa aaaaggccag gaacacaccc
    15421 gcggcgccga ggataacctc cacgcgagtg tgatttttta tgaacaatat cagcagagca
    15481 agcaggaaga aaggtaaaaa gagaaacgcc gtctgctttg aaccaagcgc tgccgccaag
    15541 ggtacgaacg atagatacag gaagaaagac tcttgctggc gaacgtacag aagcaggaac
    15601 caaaccgccg ccaggaccag cgtcgccacg agcacatccc cctggtacga gaaggtctgc
    15661 agcagcacca ctgggaaaga aaggccgagc agcccggcaa aaagcgctgc tctcctggag
    15721 gcgcccagga gcatggcaat ttcgtagatc agcaacacca ctgccagtag cgccagccag
    15781 ggcaccaggt aaaacaactt ctccgacctg cccagtaaaa agagcgatgc cccctgtata
    15841 gagatgttga ccgggtagtt caactgagtc accgtcaccg cgtcccatga agccaaagaa
    15901 ccgtgctgca gccagtaata aatgcgcggc aggtgagtgt gcaaagaatc agagttgttg
    15961 ataggcgcga gggcgccgat ataaagacac agcagcagga taccggagac cagggcaacc
    16021 aggaatattt cccagccgta taagcgccgg aattcaaact tgagcttcgg aatagggttt
    16081 ttgaatacct tttttggggg atcccatatc aacatcgcca gagccagaca gaagagcagt
    16141 tgcaacagca ggtacatcca gcggctattg agcattccta gcaggctcaa aacctcagcg
    16201 atcagtacgt tcagtgtaaa gcttgaaaga aagaaaatta tgactgagtg tgaaaatgaa
    16261 acgctcttga agattcgcag gctcaacagc agggcgagga atagatacac aagagcggaa
    16321 aagatcaaca tggaaaacct ctgagaaatt taccggtctt catgccgggt gaaggactgg
    16381 tgagagggga accctaaccc ctccgcgttc gatccgttca tgcagccaca tcattagcgg
    16441 tcccttcatg ctcggcttct taggcatccg ccgcaccagc ggttcaaccg acacgctgga
    16501 ggtatccaac gtgatggttt gtatcaggaa ctgcattgcc tggataatgg acggtacggc
    16561 aataaaaacg atgccagtgg tggtgaaaac ctcactaagg tcgggttcaa gccacttcac
    16621 tgtgccccaa attcccccca aaaaaaccag catcagactg gcgatcagaa aaaaagatac
    16681 ataagcagga atgaacacag cgatcagcgg cggggacgcg ttcgcaacgt tttctttgtg
    16741 aatttcaccg agcatgcact tattatactt gtgttgccta ttgaatccgg caaaaattaa
    16801 aacaacccct gttgaatggg gttgttggtg aaaattgaga gagacttaca cccaacctct
    16861 taactgcatg gccttgacga cgcggtcgat agccaccatg taagcggcgt cgcgggtata
    16921 taccttgctt ttctggctca tgtccagcac accagcaaag gcgattgtca tcttggtatc
    16981 caacttctca agtacttctt ctttggtcca gtagtagttc atatcattct gtaccccttc
    17041 aaagtaagaa acggtcacac caccagcgtt gcacagaaag tccgggataa caaagatgtc
    17101 gctctgtttc agcacctcat ctgcctcggg cgtggtgggt ccattggcgc cttctgccaa
    17161 tattttgacc cgcggtgata tctgcttgac cgtttccccg ttgatttgcc cttccagcgc
    17221 cgcgggaatg agcacatcca cgtctttgct gatccaggca ccgccgtctt cgatgacgta
    17281 tccggcttcg cgagccttga ccttgtccac cgtgccgtac tggtcagtga tggtcatcag
    17341 gaaacgcggg tctatgccgt ctgccttgct gacagtgtaa gaaaccttgt cctcgcggtc
    17401 gtagtaagaa atacagatga ctttgccttt gagcatttca gtaaacccaa tagaggcgta
    17461 ctgcgccaca ttgccaaatc cctggataga cgcggtgcat tttgcaccat ccagtttgag
    17521 gtgtttcatg gcttcgcgca cattgataat gacgccaaaa ccggtagcct cggtgcgccc
    17581 gctcgatcca ccaccgccca gcggcttgcc ggtaataacg cccggtgtga actctcccac
    17641 cagtttggaa tactcgtcca tcatccaccc catcatttgc gcggtggtgc ccacatccgg
    17701 cgctggtaca tcctggcggt tgccaatatt acgccacatc acatccaccc atccccttac
    17761 caggcgttct ttttcacctg tcgaaagggt tgaagggtca actaccacgc cgcctttgcc
    17821 gccgcccagg ggaatatccg cgaccgcgca tttccaggtc atccacgtgg ccagcgcgcg
    17881 caccgtatcg agcgtttcgg cgggatggaa gcggatgccg cctttatttg gaccgcgggc
    17941 gtcgttgtgc tgcacacgga acccctggaa catgcgcaaa ctgccgtcgt ccatgcgcac
    18001 cggtatgcgg aaagaaaatt cacgcaaggg ccagcgtaaa atttcccgca cctgcggatc
    18061 caatttaagc aactcagcca cattatcaaa ttgcttttga gccatttcaa atgcattaat
    18121 cgaggttgcc attcagttca ctcctttacc agttggaatt gaaattgatc agaagtcaaa
    18181 aataaagcgg gcacaccggt cctgtgcccg cattcaagcg tgatgattga atcgatctta
    18241 cgcacaaatc cctccgcatt tcagtgcaat aatagaatat acgagaatga aaagcgcgtc
    18301 aagatgacga acatcaggtc tttcgcaggg ggatgatgca gggtattgcc ggtcactttt
    18361 ctacccgcgc ctggcttcgg tcagttgttt ttcgagttct tctttcagca ctgcagggct
    18421 ggctttccct ccggcggcgc gcatcacctg cccgaagaac cagtttgcca gcgtctcttt
    18481 tcctgccagg taactcgcca gctccttctg gttggttgtg agcacttcct tcaccaggtc
    18541 agagataaaa ccagtatcag tgacctgcgc caggcctttc tcgctgatca cctgctgcgc
    18601 ggaggaaccg ctggccagca tctccgccag tacggtcttg gcggtgttct ggttgatgcc
    18661 gcctgcttcg attatgtcca acagttccgc caggctttgc gggctaacgc ggatatgcgt
    18721 aatttctgca ccagtcgagt tgaggtgcgc gaaaagctcg cctgtgatcc aatttgccgc
    18781 gctgcgtgct gatgcctgct taagcgccgc ggcacagtct tcaaaatatg cggcaatatc
    18841 actttccgat gtaaggatgg cagcgtcagc cggtatgagc ccgtattgtt gctgcagccg
    18901 gcgggtttta tcccgcggca gttcgggcat acgctgccga atttgcgcta tccaatccgc
    18961 ctccaccacc agcggtggaa tatccggttc agggaaatag cggtaatcgt gtgcctcttc
    19021 ctttgaacgc tgcacatacg tgcgctggtt tggttcatcc cacccaagtg tttcctggct
    19081 gaccgtttca ccgttttcca gcagagacgc ctggcgcgcc agctcatagg cgatggcgcg
    19141 ttccatcgct ctgaaggagt tgagattctt tatttccacc cgggtgccaa gctggtcgct
    19201 tccggcgggg cgaatagaca cattcgcttc gaaacgcatc accccttttt ccatattgcc
    19261 cgagttgacc cccaacgcgc gtagaatcgc ccgcagacca gttgcgtagg cacgcacttc
    19321 ttccaccgag tgcatatccg gctctgaaac gatttcaagc aggggtacac cggcgcggtt
    19381 caggtctacc agcgagcagt ctccgccgtc gtcggtgata tgggtgagtt ttccggtatc
    19441 ttcttcaaga tgcgcccgcc gcacgcggat gacacgttcg ccttcggatg tgtcaataac
    19501 cagccgacca ttctccgcca gcggttgttc gtattgcgaa atttggtacc ctttgggcag
    19561 gtcagggtag aagtagtttt ttcgagcaaa aatggaggtg tggttcacct cgcattcgag
    19621 cgccagcgcc acgcgcacgc catattctac cgcgcggcga ttgagcaccg gcagcgtgcc
    19681 gggcattcca gcgcacacag ggcatacact ggaattgggg cggctggtcg tggtatccac
    19741 aacgcggcat ccgcagaaca ttttggtctg cgttgccagt tctgcgtgca cctccagccc
    19801 gataaccgct tcgtattgca tgatgtaatg gattttatca cagccggtgc catccattac
    19861 cccagcacag atgcattagc gtgttaaaat atcagtaacc atcacgcact gccagacgag
    19921 gtgacgtatg ctgttgtgta tcgacatcgg caacacgaat atcaaacttg gtttgtatga
    19981 aggagaaaaa ctggtcactc attggcggat ctataccgat gagcataaac tggcggatga
    20041 atatgcagta ttgttactct ctttatttaa aagcgaggat atcaaaaatg aagccatcag
    20101 cggttgcgcc atttcatcgg ttgtgcctga gttgaccatc gccttcagag acctggtcag
    20161 aaggcacctc aacctcgaac cagtgattgt gggaaacgtt aaacgacccg tcattcacat
    20221 taacacctcc aaccccaaag aagtcggtcc tgacctgatt gccaacgccg tgggagcagt
    20281 ggcgctttat ggtgcgccct gtattgtcat cggctttggc accgctacca ccttctgcgc
    20341 catttcagcc agtggagacc ttgaaggtgt cgtgatcgct ccaggtgtta ttacggctgc
    20401 ttcttcgttg tttcaggctg gtgccatgct cccggcggtc gatctgcata aaccggaaaa
    20461 ggtcattggc aagaacacca ttctctcact gcaatccggt ttgatctacg gttttgccgg
    20521 attggtggaa gggcttagcg cccggataaa agcagagctg ggcggcgatg caaaagttgt
    20581 cgctactggc ggtctcgccc gtcttatggc ggacgaagta gattgcattg acgccgttga
    20641 gccggagttg accctgctcg gtgtgcgcat catctatgaa aaaaatcaaa agggctgaca
    20701 ccctggtttg ctctcaacgg taaagataaa gtctccacct tgagggttat gttgcgtttc
    20761 gtggtttttc agctgttttg gtaaattatc gttaaagttc tggtaaaagc aggcgtgatc
    20821 gcacccaatg aaccgcgcca cctgctgcga agacgaaaaa cagggtgatt cgggtgcgcg
    20881 tttatggacg ctcctgacca gattcgttga aatatttact tcgtgaacag aaaaaccgca
    20941 ggggttgagt gttgctcaac ccctgtggat ttgatcagat ttttcgtaca aacctttcaa
    21001 tgcgttcgag ggcttcctcc aactggttgt agccggtggc ataagagcag cgcgcaaatc
    21061 cttctccacc cataccaaac gcgttccccg gtaccatcgc aacacgctgt tcatgcagca
    21121 aacgggtgca aaaggtgtca tcatccaacc cggtaacact gaccttgggg aaggcgtaga
    21181 aagcgccttt cggttcaaag gttggcaacc cgatctgatt gagcccgctg acaattaact
    21241 tacggcggcg gtcgtactca gccagcattt tcagcacatg cggttctcca ctgagcaacc
    21301 cctcaagagc ggcaaactgg ctcatggtgg gcgcagacat caccatgtac tggtgaacgc
    21361 gcagcaaacc agtgatcagg tgttctggtc cagcggcgta accaatacgc cagccggtca
    21421 tggcgtaatc ttttgaaaaa ccgcccaaca gcaagctgcg ctcgcgcatc cctggcagcg
    21481 cggtgaaaca cacatgtttc acgccgtaaa cgaggcggtc gtagatttca tccgagatga
    21541 ccaccaggtc atgctcctcc gcgatcctgg ccacctcgag caggttctca cggctggcta
    21601 ccgcgccggt agggttattg ggaaagccaa tgaggatcgc cttggtgcgc ggagtgatcg
    21661 ctgcgcgcaa cgcgtcgggg tcaatatcaa aattgctttc catgtggctg ggtacttcca
    21721 ccgccacccc gccggcaagg atgaccgctg cctggtagga tacaaagcag ggcgtgggga
    21781 taatcacttc atcaccaggg ttcaccaggg caatagcggc caggttcagg gcttctgacc
    21841 cgccgacagt aacgatgatt tccttttccg ggtcatattc cacgttgtac aaagtactga
    21901 agtgcgccgc cagcgcccgg cgcagttgga taaggccata attcgaagtg tagtgggttt
    21961 caccgcgctg caatgactcg attcctgcca tcaagatggg ttccggtgtt gtaaaatcag
    22021 gctcaccgat gcccagcgag ataacgtctt tcatggtggc ggtgatatca aaaaactttc
    22081 tgatgcctga cggcttcagg ccggctactc tgcgggaaag atatcgatca gtcattgatt
    22141 ctccttttgt gtgattttgc cgtccgagcc agcggcagta acctgccagc cgtcccgatc
    22201 cgggtcatac accagccagt attcattnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22321 nnnnnnncgg cagtaacctg ccagccgtcc cgatccgggt catacaccag ccagtattca
    22381 tttccgttct cgcagaccac cttgaaacgt ttatccagcg gggtctgaat ttcatccagt
    22441 acttcgatga ccttctgcca gcgcctgccg tcccacactt ccaaagggcg cccagggtaa
    22501 gcgtattctg cgcggcagcg caccgggtct ttcagctttg cacctgggtg tggtcgccca
    22561 caaacaggcg cgcgggttgc cctttgtaca caacctcccg accgatgatc gatccctcga
    22621 tgaccatgtt ttcgatcacc gtgttgtcat ccacgatgga gttgttgatg accacattct
    22681 ttagtgtaca gcctttgcca accgagacat acgggccaat gacacaggct tcgattttcg
    22741 ctcccggtgc gatgaacacc ggcggtacaa tcgtagcacc cggtatcccg gaatctggct
    22801 gtgttgccac cctgccctga tccagcaaat ggcggtttgt ctccagcacg gcggaaggaa
    22861 taccggcatc caaccaggtg tcgatatttt ctttacgcat gcgaccgccc atttgaatga
    22921 agatgttgat ggcgtccgcc aaaaaatact cgcctttcaa gcttttccca aggcgcatct
    22981 gctcttcaat cgccctcaat aacgcggcag tatctttgaa ataataaaaa ccgaccagca
    23041 cctggttgtt gctggtatct tgcggctttt ccaccagccg tgtggcgatg ccattttttc
    23101 cgatttcgat gacgccgaaa cggcgcggat cttccactgc tttaacaaaa gccactgcgt
    23161 ccatctcttc gaagggtatg aattggaggt ctgtctcgat caatgtatct gaaaaagcga
    23221 tcaacgccgg tcctgccagg tattccttcg cgcaccataa agcgtgcgac tgtccgcgca
    23281 tctctgcctg gatgacaaag tgcaccttct tctgcgggtg cgcgtgtcgc atgtattgct
    23341 caatggcatc cccctggttg gggctggtga tgaacacata ttcggcgttt tcaacgcccg
    23401 gaagagaaga aaactgttca agcgcgtaat ccaacacggt tttaccggcc aacggcagca
    23461 gcggtttcgg acggctccag gtgagcgggc gcatgcgaga accatatccc gccatcggta
    23521 taataatctt gaatacctga ctcatggaca cctcaaccgt catctcatcc taactggtga
    23581 tcgtgtggtt acatcatctc cattttacac tgcctgcgca aattggtcga ttgaatattc
    23641 acacccaacg ggcggataaa gagcataaaa agtcccaccg cgcgcggtgg gagcaggtga
    23701 aaagcgcctt tagtatcttg ccagggtggt gatggcgggt acctgatcga acatccaaaa
    23761 agtcaaagga aacgccagcc caaaggtgac cgcggtgagc agccaggcca gcagcatcca
    23821 caccaggctg aaccagaaac caatgaccaa gaagtacagt gcgcgcagca gaaacgggtt
    23881 ttgacgtatc gttgtctgcc ggatcaccgg agaaccctcc ctgatctcca cagtggtctg
    23941 cacgcgttgc ggacgcaggg tcatcacctg cgggatacgg ttgatcatga gcagcccgat
    24001 aggcagcccg acaatggtaa ggttgaagaa ccaggcaacg accacccaaa tgagcccgag
    24061 ccaaagacca atgaacagaa aatacagacc gcgcaccagg cagcccggtc cccgctcgat
    24121 gagttccact cttgtgttct gctgagtcat gtttgatact ctccttttcc attcatatac
    24181 gcaaataaaa cactgctgtt gcaggtcagc agaaagggag agagaaaatt cacagagcgg
    24241 gttcagtcga gggcgggcac ctgttggtgc cagtctgtta caccctggta ggcatgcgcc
    24301 gcgcggaaga gcacatcttc acggaaatgc gctccaagga actggatacc aatcggcagg
    24361 tggttgtcat cgaaccccac cggtaaagcc agcccgggaa tgcctgccag gttggtaggc
    24421 aatgtgaaca cgtcctccag gtacatggct agaggatcgc cggcgtgttc acccagttta
    24481 aaggcagtgg atggcgccac cgggcaggcg ataaggtcca catgttcaaa agcctggtca
    24541 aaatcgcgcc tgatgagcgt gcgcaccttt tgcgcctgac cgtagtaggc gtcataatag
    24601 ccggcggaga gcgcgtaagt gcccagcatg atgcgccgct tgacttccgc gccaaaaccc
    24661 acgccgcgcg accgtttgaa catttcccat agcgttgcag ctggttcgcg cagaccaaag
    24721 cgcacgctgt cataacgcgc caggttggcg gaagcctcgg ctggggcgat gaggtaatag
    24781 accggcaggg cgtactcggt gtgtggcagg ctcacctcca ccagctcagc gccaagcaac
    24841 ctcaggatat caatagccgc aagcacacac tggcggacct gtggttgaat gccttcaata
    24901 aagtattcct gtgggattcc cacgcgcagc ccatccagcc gctccttttc ccgcaggtta
    24961 agaaccggaa cggggacatc aacggtggtg ctgtccagcg ggtcaatacc ggccataagg
    25021 gagaataaca gcgctgcgtc ttccgcgttg cgcgtgaaca cgcccatcga atccagagag
    25081 gagccgtagg ccaccaaccc ataacgcgag acgcgcccgt aagtcggctt gataccgcag
    25141 acgccacaga aggatgctgg ctgccgaatc gaaccgcctg tatcagagcc cagcgcagca
    25201 ggtaccatac ccgctgccac agcagctgcg ctgccgccgg aagagccccc tggtacacgc
    25261 gtgacatccc aagggttgaa ggtaggacca aacgcggagg tctctgtgga agaccccata
    25321 gcgaactcgt ctgtgttggt cttgcctact acaatggccc cggcgtctag caaccgctgc
    25381 accgcgctgg cggtgaaggg cggcacaaaa tctttgagaa tggccgagcc gcagcaacaa
    25441 cgcattcccg caaccgtgat caaatctttg actgcgatgg gcagccccag caggggcggg
    25501 gtttcgctac cgctgtggcg tgcagacgcg tagcgggcat ccgccttatc cgcaaggctg
    25561 agcgcgtctt cctccgccag ggtgacgaaa gcgtgaacac gcggctccag gatcgcgatg
    25621 cgttccaggt aagcctgtac cagctcgcgg caggaaaact ccgccttttt cagtccatcc
    25681 agaatctgta taacggaaag atcggtcaag ctcgtgttca ttccagcacc ggcggcacgc
    25741 gaaactggtc tttcattttc tcgggagcgt ttgccagcac gctttcaacg gtcagccctg
    25801 ggcggggttc atcgacgcgc agcctgccgt tagcagggag aacgcttgaa gtgggtggta
    25861 cgccttctgt gtccagcgcc tgcaaccgcg ccacgtgttc caggatggcc gaaagttgct
    25921 gccggtactg ctctttctct tccgcggtca ggtcaaggcg tgccagttcg gcgatatgtt
    25981 ctacttcaac aagggataat ttcatgcgtt gattatacct gagtgccgga ggttcattcg
    26041 actgacccag aggattaatt tgcagattga ggaaaaatag cctatactaa gtgtacattt
    26101 tttgaaggga taaggaggat agaaatgcca agcattatcg atattgaagg ga
//
LOCUS       DAOSPM010000015        26106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1872_length_26106_cov_13.485778, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000015 DAOSPM010000000
VERSION     DAOSPM010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 26106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26106
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1872_length_26106_cov_13.485778"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..76)
                     /locus_tag="PLW25_02620"
     tRNA            complement(2..76)
                     /locus_tag="PLW25_02620"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Pro,seq:cgg)
     gene            157..960
                     /locus_tag="PLW25_02625"
     CDS             157..960
                     /locus_tag="PLW25_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53413.1"
                     /translation="MKRPSLPLLLFICWLLAACAAPATPAVLPPTFTIQTAAIASTSL
                     PSPTSAPTSTATPATPAPPAVTLPQGALSAQQRQALENAARAFISPSPEEALATAIEL
                     GYLGRNANPATMCGPLALAILQQSGLVDSSFPLKSFYYLNPRPGHDERYLEKVFPADR
                     FEKIVREESIGKIDFSADPLLPGDFLYLFAGDSGSFEHMLVVSRVDDAGRAYAVTNLN
                     TARGVIVDEVLLYDPANPGVGQFYQWTDWENRMIGRTGYGGYWLWRPLP"
     gene            1053..1412
                     /locus_tag="PLW25_02630"
     CDS             1053..1412
                     /locus_tag="PLW25_02630"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOS53414.1"
                     /translation="MTSCVFCDIISGSAPSDQLYADEELLVIQDIHPVAPVHVLVIPR
                     KHIPSLNHLQPEDQALASRLLLTVPRIAHQLLGDDAAYRTIINTGAAAGQTVFHLHVH
                     IISGRPFINHLLTRGLY"
     gene            1412..2938
                     /locus_tag="PLW25_02635"
     CDS             1412..2938
                     /locus_tag="PLW25_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOS53415.1"
                     /translation="MHQSARLLFAILATLLISGCIKPAPGSVPWQISGTQELPGAQHP
                     VTSSPPSWMPATRVPGSPYFTPTPDTPRVMPTLRADQEFYTVQYGDSLGNIALLYNLD
                     LASLIAANDLANPDILEIGQVITIPAPQPGAEPSSFKIIPDSELVYGPVSSTLDIEAF
                     VNERNGYLNAYREEVEKIEMSGAQIVARVSREFSINPRLLLALLEYRSGWVTTSLIDD
                     SLRDYPMGLQDPARKGLYLQLAWAAKLLNRGYYLWKINAISFTSFANHSLALLSPTIN
                     AGTAAVQYMLGVGSYPDAWQRAVSEEGLFRTYCELFGIPFDLAFEPLIPADLQQPALT
                     LPFEPGTLWSFTSGPHAGWADGSAWAALDFAPPGEQLGCYITDEWVTAVADGLIVRSS
                     SGAVVLDLDGDGLEQTGWTILYMHIETRDRVAAGARVKTGDRIGHPSCEGGFSNGAHV
                     HIARRYNGEWISADREIPFNMSGWVSSGDGSEYSGTLTKNGKVVYSWNGRVDANQIGY
                     "
     gene            3048..4511
                     /locus_tag="PLW25_02640"
     CDS             3048..4511
                     /locus_tag="PLW25_02640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOS53416.1"
                     /translation="MADTTAAPTEETPFTANISLFQTPTQPPLPTFTDLPAFTSTPQP
                     SRTPDPNATPKPPLQYYTQAGDTLASITGRFGVSADQIIPSEPIPVDGLINPGVLLII
                     PDVLEDVYESLTLLPDSEVVYSPSAVNFDILKYVAEADGYLNRYQETVGSRQYSGAEL
                     VQRVALENSINPYLLLTLLEYKSHWVSSRPTNMAESEYPMGYVRLEYRGLYKQLSWAV
                     EQLSIGYYGWRAGSLNSLTFKDGSSVRISPGLNAGTAAIHYLFSRWYNQAEWAAALYG
                     ADNMPDLMSHMFGDLWERSRAVDPLYPADLQQPEFSLPFQPGRVWSFTGGPHSAWGEG
                     GALAALDFAPPSSTAGCVASNEWVTAAASGVIVRAADGIVIQDLDGDGVEQTGWNIMY
                     LHVETRDRVQPGTYVQTGDKIGHPSCEGGISTGTHVHVARKFNGEWILADGPLPFTLS
                     GYVAKNGGSPYQGSLESGQARIIANPLGTLRSYISLP"
     gene            4614..6053
                     /locus_tag="PLW25_02645"
     CDS             4614..6053
                     /locus_tag="PLW25_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOS53417.1"
                     /translation="MSILILSACSPQPPITVYITPFPLSATEPPAQQPTALATRPVYQ
                     PGTLVAYTARTGDNLPALAHHFNTTVKEILENNPQLSEKTTTLTPGENLQIPIYYEAL
                     WGNSFQIIPDSLYINGPAQIGFDTKSFVNAHPGWLKNYASLAGGEVRRGADLIDYVSL
                     EYSISPRLLLALAEYQAGALSVETLEPQEKVYPLGYEDEFHQGFYLQLLWAANTLNNG
                     YYSWRSGKLGTITRLDGSIEHPDPWQNAATVALQNYFSQVLPVADYHKAIYSEGLYNT
                     YAALFGDPWQHTSDHIPGDLQQPALRLPFVPGKTWSYTGGPHSAWGEGAPLAALDFAP
                     PSVVGGCAASNEFVTAMADGQIVRSEKGLAMLDLDGDGDEHTGWVILYLHLGLNDKIR
                     PGMLVKTGDLIGHPSCEGGSATGTHVHIARKYNGEWIEADSVVPFNLEGWIAKNGVVP
                     YQGSLSNYGRIITASDKGLVNSYITAGNP"
     gene            6053..7057
                     /locus_tag="PLW25_02650"
     CDS             6053..7057
                     /locus_tag="PLW25_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53418.1"
                     /translation="MHERTRSGQIRLESIRLIFGLLLLAMVTSGCVANPVPDEPAPGS
                     GQPTAASTLESNTLETSDAITAAVTATADLPATDSQTPPPQAEAEADWLSMPIVPTVS
                     PRMKDVFERGQKSGRDAARFSKIGDCQNITTYFLAMYDSGNYRLGDQYAYLQPTIDHF
                     KGSWWRQSLSVKGGMNVAAVLSPIWANPDKCLPKETPLACELRVYNPAFAVISLEESW
                     SGSIEHYDMYLREIVEYVLAQDIVPILATRAETETQERQINPTVARIAHDYQVPLWNF
                     GAAARALPNNGIRPDGFHITEGQSYFDDEAMLKTGWTQRNLTALQAIDAVYRGLTQEP
                     "
     gene            complement(7108..7524)
                     /locus_tag="PLW25_02655"
     CDS             complement(7108..7524)
                     /locus_tag="PLW25_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOS53419.1"
                     /translation="MDAKLRWKYGLSFKGVADSNFEVPLGASVDHGGAGDGVVPMELI
                     LMGLGGCTAMDVISILEKKRQQVTDFEVKLHADRAGEHPKVFTDITIEYIIKGRNIDP
                     DAVARAVDLSENKYCPGMAMLRKAANIQTKYTIEQA"
     assembly_gap    7980..8079
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8080..8363)
                     /locus_tag="PLW25_02660"
     CDS             complement(<8080..8363)
                     /locus_tag="PLW25_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS53420.1"
                     /translation="MSAARQFPSEFVDRLIAGDGQAFNELVDQTSAGIYGLGLRMLGN
                     AQDAEDMLQETFLKAHNALRGFEKRSSLYTWLYRIATNEALMMLRRRKSE"
     gene            complement(8392..9420)
                     /gene="tsaD"
                     /locus_tag="PLW25_02665"
     CDS             complement(8392..9420)
                     /gene="tsaD"
                     /locus_tag="PLW25_02665"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOS53421.1"
                     /translation="MKLKSARLLGIETSCDETAAAVVENGREILSNVVATQIDLHTKY
                     GGVFPEVASRQHIRTIYPVIDEALQKANLGLADIDAIAVTRGPGLAGSLVVGMNAAKG
                     LALGRQLPLIGVNHLEGHIYSAWLYPADTPKPPPPPEFPQLALLVSGGHSELNLVQDH
                     LQYQRLGGTLDDAAGEAFDKVARMLRLPYPGGPAIQKAAREGNPQAFPFPRSRLENTW
                     NFSFSGLKTAVLREVRALEEENRSLPVPNLAASFQEAVVSVLFEKTMKAARDYAVKSI
                     LVAGGVSANEALRKTFTSQTEFPVHIPPLSLCTDNAAMIASAGYYHFCLGHVDPLDLD
                     VLPNWPLS"
     gene            complement(9447..10682)
                     /locus_tag="PLW25_02670"
     CDS             complement(9447..10682)
                     /locus_tag="PLW25_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOS53422.1"
                     /translation="MKTDLDKLMKKHNVDAIFIIGAAQHNPSMVYFVGTAHVSDAILI
                     KQRGKDAVLFHYPMEREEAAKSGLKLVSMTKYPLHDLLKQAGGNYLEAFVLRLKLMLE
                     EMKLTRGRVALYGKDDIGPFYSILNRLQELMPELQFAGFVRDEILMNAMMTKDAGEIE
                     HIRAMGRVTTDVVAKTADYITGHKTKGGVVVHKDGTPVTIGEVKNLIQLWLAERGAEN
                     PEGTIFAIGRDAGIPHSVGNPSDALQLGQTIVYDIYPCEAGGGYYYDFTRTWSLGYVT
                     DEAQQLYDQVRQVYDTISAELKVNAPFYKFQERACALFEEMGHPTIQSDPTVERGYVH
                     SLGHGIGLRVHEKPWASAVNPSPTDILAPGSVFTLEPGLYYPERGMGVRIEDSWWVTP
                     DGEFEILAEFPKDLLLPMK"
     gene            complement(10679..12037)
                     /gene="selA"
                     /locus_tag="PLW25_02675"
     CDS             complement(10679..12037)
                     /gene="selA"
                     /locus_tag="PLW25_02675"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOS53423.1"
                     /translation="MIDLRKLPSVDRLLLHAHAAPMLDCYGRSLVTTNIQNVLESIRQ
                     NPQAETFEPTEEAILERVKNQLIQALTPTLTRVINASGVVLHTNLGRAPLSRSAQQAM
                     SEIAGGYATLEYDLEKGKRGSRSVHASGLLRRLTGAEDALVVNNNAAAVLLCLSALAS
                     RRAVVISRSQLVEIGGGFRIPEVMKQSGARLVEIGTTNRVNLGDYESALRDGAALLMR
                     AHTSNFKLVGFTSQPELKELTALAHQYEVPMLDDLGSGALLDTSRFGLAHEPMVQESL
                     AAGVDLVCFSGDKLLGGPQAGIILGKAHLVAKIKRHPLARAVRADKLCLAGLEATLLH
                     YLKGEAEKEIPIWRMISASANDLKERATRWQQQVGGEVVEEHSTVGGGSLPEETLPTF
                     ALSFNIPGLEQMAARLRQSTPPLIARVQGGRLYLDPRTVLPEEDEDLVRILRTHITGA
                     HR"
     gene            complement(12034..12444)
                     /locus_tag="PLW25_02680"
     CDS             complement(12034..12444)
                     /locus_tag="PLW25_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53424.1"
                     /translation="MKDTQPPRMLFMPVLTTSIILVLTSTVGLMGLFLFTVPTLGPRW
                     LLFFFVTLLASGLALPAIFYLHLRFPSNPPVTSKVIIREAAWWGIYADILLWMQFGRV
                     LNFALAIFIAIGFIAIELLIRMRELNRFPSRMKP"
     regulatory      12613..12713
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            12787..>15202
                     /locus_tag="PLW25_02685"
     CDS             12787..>15202
                     /locus_tag="PLW25_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOS53425.1"
                     /translation="MDRTFTNRQYLDALQSKVMVFDGAMGTNLQAQNLTADHFGGEKT
                     NGCNDYLVITYPQAVEKVHRSFLSVGVDVIETCTFRSNRLTLSEYGLSQEVTAINQAA
                     AALARRLADEYSQTGQPRYVAGSIGPSGKLPSMSDAELSNITFDELTSIFKQQAEGLI
                     LGGVDLILIETSQDILEVKAAIHGIQQAFSSSGVFLPIQAQVTLDTTGRMLLGTDITA
                     VLAILEGLPIDVIGLNCSTGPDFMREPIRFLGEHSTLPVSCIPNAGLPLNVNGQAVYP
                     LEPEPFAQALYNFVVNHGVSVVGGCCGTTAAHLARLVEKVRAIQPGPRLRISSARLSS
                     AMQAQPMQQEPPPFFIAERLNTQGSHVFKNMVLENDFESILTLAQQQMESGAHGLDLC
                     VALTERADEKETMQRLIKTIAPVINLPLVIDTTEVDVLETALKTAPGRCLINSTHLEG
                     GRQKADRIFGLAKTHNAAVIVLTIDEQGMAKTRERKLEVARRIYDIAVKEHGLAPHDL
                     VFDDLTFTLATGADEFTNSAIETLEGIRLIKAALPGVLTSLGVSNVSFGLAANARKVI
                     NSVMLYHCVQAGLDMAIVNPATITPYPDLSPEERELAEDLIFNRKPEALQRLIEFFQA
                     AGPSSAQQNSQQEALSMMTPGERLHWKIVHRAKEDLETDIDTLLQMREKEVSRNDAAV
                     GILNHVLLPAMKDVGDKFGRGELILPFVLQSAEVMKKAVSYLENFLERKEGASKGKLL
                     LATVYGDVHDIGKNLVKTILSNNGYNVVDLGKQVPADTIINRAVEEEADAIGLSALLV
                     STSKQMP"
     assembly_gap    15203..15302
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15303..16166
                     /locus_tag="PLW25_02690"
     CDS             <15303..16166
                     /locus_tag="PLW25_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOS53426.1"
                     /translation="LERAAAEVKPISVQAEKTVEPAALIPSPPGYGVRVVEDMPLEIV
                     FKHLALNELFRLSWGAKNAHGAEWEKIKTEYEKRLALMQKQAIAEKWLKPRGVYGYWP
                     AQSDGNDLLIYDGDSVEKGNPIVINRFTFPRQSSGEFLCLADYFASVDSGRMDVVAFQ
                     VVTVGKEASERFDRLQAVADYAEAYFSHGLSVQTAEATAEYLHRHIRHELGLSENQGK
                     RYSWGYPAIPELEDHKKVFALLPAEAALGMSLTSACQLVPEQSTAAIILHHPQAKYYS
                     LGETRVEQLMK"
     gene            16177..18051
                     /locus_tag="PLW25_02695"
     CDS             16177..18051
                     /locus_tag="PLW25_02695"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HOS53427.1"
                     /translation="MINQATLLNLLAKKRALFSDGAMGTMLSQNLPTGLTCLDYANVE
                     YPRVVESIHREYILAGSDMIQTNTFGANRYKLAQHHLENQLEQINTAGAQIARQAVSA
                     SGREVLVAGDIGPLGVRIAPFGRVQLPEAHEAFSQQATALIKAGVDLIIIETMTDLYE
                     TVEALRAVREISPQLPVIASMTFTRDNRTLLGDPPTRVSQQLHAAGADVIGVNCSGGP
                     NQILNILRQMRRAVPDAIFSVMPNAGWPEQVEGRIMYPAAPEYFGTYALSFWQSGASI
                     IGGCCGTTPAHIAAMVQAFQQSPGLERSAIEEIALSATRQPEDEGALPPTKLAQKLRD
                     GRFVIAVEMDPPRGLSTHKLIAGASLLSEVGADVIDVADSPMARMRMSPWAVCSLIQR
                     NVGIETVLHFPTRGRNVLRVQGDLLAAHALDVRNIFVVMGDPTSIGEYPDAMDHYDLV
                     PSGLIKLVKQGFNAGVDHAGSGIGQPTSFFVGCALNLKAADMEAELKNLRRKLKAGAD
                     FILTQPIYHPALVTDFLQQYESQFDKLPVPLIVGLLPLASARHAAFLQAEVPGIEIPQ
                     SIRDLMAVSGDQGAQTGIRLTIELIQNIKPMVQGIYLMPAFQRYDYAAEIIEKTRTAE
                     "
     gene            18099..18830
                     /locus_tag="PLW25_02700"
     CDS             18099..18830
                     /locus_tag="PLW25_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOS53428.1"
                     /translation="MKILVISDVHANLTALEAVLADAGNNYEVVWCLGDLVGYGPDGN
                     QCVQRIRELPNLLCIRGNHDLAICGDADLSIFNHEAGEAIIISRRMMSPETLQYLRSL
                     PEKQVSGQATLIHGSPRNPVWEYILDVSIAAENFASFDTQLAFVGHSHLPLMFTADPQ
                     SGLVERAYRYPGVTTPVTTRAILNPGSVGQPRDNDPRASYGIFDPQNMTWEIHRVPYD
                     IPSVQQRIIAAGLPEKHARRLSAGW"
     gene            18904..19896
                     /locus_tag="PLW25_02705"
     CDS             18904..19896
                     /locus_tag="PLW25_02705"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOS53429.1"
                     /translation="MKTKSVLIGIVILSLTLSGCASKSYTANNLRSGGVEDYAMKAAP
                     MVEPMEPMLDSYAESAGSPGIAAAGVESVQMIMMNASLSIAVDDPAATLRDVQQLAKG
                     MGGYTVSSNLYKSYTSSGVEVPQANLIIRVPAERLDEAMQKIKDMTGDPKKYTLNESV
                     SGQDVTKEYTDLKSRLRNLEEADAKLSEFYENAANTEDALSIYSQKMQVTEQIEIIKG
                     QIKYYDESVSTSSITLQIQSKETIAPITVAGWQPSGVARDALQALLDFGKGLVNFLIW
                     LVILVVPIVLLFGLPIFFLVRWLKRRSTARKLAALERRAQENKSEPTPPIPTKK"
     gene            complement(19943..22285)
                     /locus_tag="PLW25_02710"
     CDS             complement(19943..22285)
                     /locus_tag="PLW25_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS53430.1"
                     /translation="MEQLNSVSLMLQVNPDSGTFSIQARDPRFPRLENIQLGCEYTFA
                     GKQQRCGQDGWQVNGLAHHLERAQAHGEMDCLTVALEPDANKVSLRLSFALMREYPLV
                     LWKIQVTNHGKEGVEINRITLLRVVDNTHYLKVRRPDDLGFFSNGWQSWSPSRWYSGN
                     QRMTISRLYALQRPMIYNPGTPTPVKKGVFSSDMFAVVGDRATRAGYLLGFLSQLNHF
                     GSILADFNTGEISMWANGDGACLEAGKSMQTDWAVFTPVLLDHRAPLETYIEAVGREN
                     HARVPAQTPVGWCSWYHFYTSVTARNIIENLDTILERQEELPIELVQIDDGFEKQVGD
                     WLEFRSTFPEGVAPLAASISGGGLLPGLWLAPFIVHPGADLLHKHPDWMLRKANGRLV
                     NAGLGWNALTTALDLTKPPALQYAAEVVHTAVQKWGFPYLKLDFLYAAALKGHYYDRS
                     KTRAQVMRMGMEALRQAAGEQTTLLGCGAPLGSVIGVVDTMRIGPDVSEEWLPVFKGV
                     QIGLRREPSMPSTRNSIHNILTRANLNRRWWVNDPDCLLVRADTSLTQEEVRSLATVI
                     ALTGGSLLLSDDLPGVPPERIRLAEALIPVMGEEVRVLDWFDNATPSRLRVDMDNETG
                     GWHLLGGFNWSEQTADVMISASEYGLEEGEYLFREFWSGKTGMISGTGGHVFPGIPPH
                     APVLLAVRRFVGEKALYLGSDLHFTQGAEVAEWLEKDGSLEMTLRLPRTAIGQVFFHL
                     PGKPQAAFVDGKPVYLHPMEQGIYRLPVVVEGFCNVRIMR"
     gene            22383..25175
                     /locus_tag="PLW25_02715"
     CDS             22383..25175
                     /locus_tag="PLW25_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOS53431.1"
                     /translation="MPPMVAIDIETTGMDAQRDAIIEIAAVRFNGHRVEGEWTKLINP
                     GRPIPRQITQLTGITDQMVLHAPPLQAVIQELVDFVGNDIVVGHNVRFDLSFLQRQKV
                     LLYNKVIDTYEMAAIALPGNSRYNLGTLGVTCNILIPNSHRALDDARLAQAVYYSLSQ
                     KLLDLPIEILDEIVRMSEGIEWDGEVAFHLALQAKGRLPIEARKASQDDFGLLFAGTQ
                     SFPPLVPVETPVRIDPDEIAARIEHGGPFSRYFDNFESRPQQLEMLRSVANALNNSQH
                     FIVEAGTGIGKSFAYLIPAVEWAVRNSMRVVISTNTITLQDQLIKKDIPDLASALGSE
                     VRATVVKGRGNYLCPRRFSAMRKIGPQNAEELRILAKTLVWLYQGGSGDRGEINLNGP
                     LEREVWQRLSAEDEGCKTEVCVSRMGGACPFYRTHLTAQSAHILVVNHALLLADVVTG
                     NRVLPEYNYLIVDEAHHLESATTNALSFRLTQNDFTRMLREIGGISSGLLGRLMSLVS
                     HALPPSDFAALNMAVHKVSDLLFQLENEFSELIFSLDAFMDEQREGQPVSSYGQQCRV
                     LPATRTLPCWSNVEIAWDSCQDALGAALKMIADLQKAIGDSLDEVPQEVEDTLGSLAS
                     VYRRLSEAQNYLEALVNHPDANYVYWVEIMPNTQRLALQAAPLHVGPLMEKYLWHDKA
                     SIILTSATLTANNEFEYIRSRLNADEADELVLGSPFDYENSTLLYLVNDIPEPAEASA
                     YQREVESAIIRLAKATGGRMLVLFTSYAQLRKTAQAIGPAMADAEIQVYEQGEGASTN
                     ILLENFRGADKAVLLGTRAFWEGVDIPGEALSVLVIVKLPFDVPSDPIIAARAETFED
                     PFNEYNLPEAILRFRQGFGRLIRSQSDRGVVAILDKRVLTKKYGRMFLDSLPECTRRV
                     ASSLELPAAAARWLNI"
     gene            25229..>26106
                     /locus_tag="PLW25_02720"
     CDS             25229..>26106
                     /locus_tag="PLW25_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOS53432.1"
                     /translation="MMRYVFSSMRPRFLLLALACVFLGTAAAIWAVGKVNPWYVLLAF
                     IGGLAAHACVNAFNDYNDIRSGLDFRTNRTPFSGGSGTFVEEPEKVHLALWTTIGTAL
                     ITVVIGIYFALAVGWIILPIGILGLIVILTYTQWLNKQPLLCLLAPGFGFGTLMVLGT
                     FYVLTGRITWSAVLTSSIPFFLVSNLLLLNQFPDVEADTTVGRKHYPILIGRKTSAII
                     YGCFLAATYLAIILGVMLRITPAWTLLGLLTAAIALPTALKVLQHAENIPALVPSMIQ
                     NVLLNLVTPLLMGVGF"
BASE COUNT         5793 a         7593 c         6790 g         5730 t
ORIGIN      
        1 gtcggggcgg gcggatttga accgccgacc tctcggtccc gaaccgagtg ctctagccgg
       61 actgagccac gccccgaacg cctgcattat accctaaccc ggaaaaatca gagagggttc
      121 gcgcttccgg cttttattcg ttacaattaa cctgttgtga aacgaccttc cctgcccttg
      181 ctgctgttca tctgctggct gcttgccgcc tgcgccgcgc ccgcaacgcc cgccgtcctg
      241 ccgccgacgt ttaccatcca gacggctgcc atcgcctcca cttcactgcc cagcccgacc
      301 agcgcgccca cctccaccgc cacgcccgca acgcccgctc cccccgctgt aactctgccc
      361 cagggcgcgc tcagcgcgca gcagcgccag gcgctggaaa acgccgctcg cgctttcatc
      421 agcccctctc ccgaggaagc gctcgctacc gctattgagc tcggttacct ggggcgcaac
      481 gccaatcccg ccaccatgtg cggcccgttg gcgttggcga ttttgcagca atccggtctt
      541 gtcgattctt ctttcccttt gaagagcttt tactacctca atccacgccc cggtcatgac
      601 gaacgctacc tggaaaaggt ctttccagcg gatcgatttg agaagatcgt gcgcgaagaa
      661 tccattggca aaatcgactt cagcgcagac ccacttttgc cgggtgattt cctctatctg
      721 tttgccggcg acagcggcag ttttgagcac atgctggtgg tctcgcgcgt agatgatgcg
      781 gggcgggcgt acgctgtgac caacctcaac actgccaggg gggtgattgt ggatgaggtg
      841 ctgttgtatg atcccgccaa ccccggcgtt ggacagtttt accagtggac agattgggag
      901 aaccgcatga tcggacgaac cggttatggc ggttactggc tctggcgtcc gctgccctga
      961 ttttccccct tcaccccccc acacacacac ttgttgccgc tttctattca tggataaaat
     1021 tcaggcctga atattgcaac cagatacaag atatgaccag ttgcgtgttt tgcgatatca
     1081 tctccggcag cgccccttca gaccagttat acgcggatga agagctgctc gtcatccagg
     1141 atatccaccc ggttgcgccg gtgcatgtcc tggtcatccc gcgcaagcac atcccctcgc
     1201 tcaaccatct ccaaccggaa gaccaggcgc tggcgtcccg tctgctgctc accgtgccgc
     1261 gcatcgccca tcaattgctg ggagatgacg ccgcttaccg tacgatcatc aataccggcg
     1321 ctgccgcggg gcaaacggtc ttccacctgc atgtgcacat catctccggc cgtcctttca
     1381 ttaatcatct gctcacccgg gggttgtatt gatgcatcaa tccgcccgcc tgttgttcgc
     1441 gatactcgct acgctgctca tcagcggctg catcaaacca gcgccggggt cagtgccctg
     1501 gcaaatttcg ggcacgcagg aacttccggg tgcgcagcat cccgtgacct cctccccacc
     1561 ttcctggatg cctgctaccc gcgttcccgg atcgccttat ttcacaccca cgccagatac
     1621 gccgcgggtt atgcccacct tgcgcgctga ccaggagttt tacacggtgc agtatggcga
     1681 cagcctgggg aacatcgccc tcctttacaa cctcgatctg gcgtcgctca tcgcggcaaa
     1741 cgacctggcg aacccggata tcctggaaat tggtcaggtc atcaccatcc ccgcaccgca
     1801 acccggggcg gaaccttcat cctttaagat aatcccggat tccgagctgg tttacggtcc
     1861 ggtcagttcc acgctggaca ttgaagcgtt cgtgaacgaa cggaatggct accttaacgc
     1921 ctaccgcgaa gaagtggaaa aaatagaaat gagcggggca caaattgtgg cgcgggtaag
     1981 ccgggagttt tctatcaacc cccgcctgct gctggcgctg ctggaatacc gttctggttg
     2041 ggtgactact tccttaatcg atgattcgtt gcgcgattac ccgatggggc tgcaagaccc
     2101 ggcgcgcaag gggctttacc tgcagctggc atgggcggcc aaactactca accgcggtta
     2161 ttacctttgg aagatcaacg cgatatcttt caccagtttt gctaaccaca gcctggcgct
     2221 gctttcaccg accatcaacg ctggtacagc agcggtgcaa tacatgctgg gggttggctc
     2281 gtatcctgat gcctggcaaa gagcggtctc agaagaaggg ttattccgca cctactgcga
     2341 attattcggc atcccatttg acctggcttt cgagcctttg atccccgctg accttcaaca
     2401 gccagcgctc acgctgccct ttgaacccgg taccctgtgg tctttcacca gtggccctca
     2461 cgccggttgg gcagacggct cagcctgggc cgccttagac ttcgcccctc cgggtgagca
     2521 gctcggctgc tacatcacag acgaatgggt aaccgcggtt gctgacgggc tcatcgttcg
     2581 ttccagtagc ggcgcggtgg tgctggacct ggatggtgac ggcctggagc agaccggttg
     2641 gaccatcctc tatatgcaca tcgaaacccg cgaccgcgtc gcggcaggcg cacgggtaaa
     2701 aacgggggac cgtattggcc acccttcgtg cgaagggggt ttctccaatg gcgcacatgt
     2761 tcacattgcc aggcgctaca acggcgaatg gatttccgct gatagagaaa tcccattcaa
     2821 tatgagtggc tgggtctctt cgggtgatgg cagtgaatac agcgggacgt tgaccaaaaa
     2881 cggtaaggtg gtttattcct ggaacggacg ggtagatgcg aaccaaatcg gttactaggc
     2941 tctttcagat agtcgccgcc tgggcggtat tagccggctc aatgctagct tgctcgcagg
     3001 gttacgtgag cccggcggag cttacagcta ccgctggcct ccgccccatg gcagacacaa
     3061 ccgcggcgcc tacagaagaa acccctttta ccgccaacat ctcgctgttt caaacgccaa
     3121 cccaaccacc cctgcccacg ttcactgacc tgccggcgtt taccagcaca ccgcaaccct
     3181 cacgcacgcc agatcctaac gctacgccca agccgcccct ccaatattac actcaggcgg
     3241 gtgatacact ggcttctatc accggtcgtt ttggcgtcag cgccgaccag ataattccca
     3301 gcgaacccat cccggtggat gggttgatca accccggggt gctgctcatc atccccgacg
     3361 tgctggaaga tgtgtatgaa tcgctgacct tgctgcctga cagtgaagtg gtttattctc
     3421 cttctgcggt gaatttcgat attctgaagt acgttgcaga agcggatggt tacctcaacc
     3481 gttaccagga gacagttggt tcacggcagt acagcggcgc ggagctggta cagcgcgtag
     3541 cactggaaaa ttcgatcaac ccctaccttt tgttgacctt actggaatac aaaagtcact
     3601 gggtcagcag ccgacccact aatatggcag aaagcgaata ccccatggga tacgtgaggt
     3661 tggaataccg cggtttgtac aagcaactct cctgggcggt agagcagctt tccatcgggt
     3721 attacggctg gcgggcgggc agcctgaact cgcttacttt taaagatggt tccagcgtgc
     3781 gcatcagccc gggtttaaac gccggcaccg ccgccattca ttacctgttc tctcgctggt
     3841 acaaccaggc tgagtgggca gccgcgctct atggcgctga taacatgccc gatttgatga
     3901 gccacatgtt tggtgacctg tgggaacgct cacgcgcggt tgaccccctt tatccagcag
     3961 acctacagca gcctgagttc agcctgccct tccagcccgg ccgcgtgtgg agcttcactg
     4021 gcggaccgca ctccgcctgg ggtgaaggtg gcgcgctggc ggcgcttgat ttcgccccgc
     4081 catcaagcac cgcgggctgc gtggcctcaa atgagtgggt gacagcggct gcctccggcg
     4141 tcatcgtgcg tgcggcggat gggattgtga ttcaggacct ggatggagac ggcgtcgagc
     4201 agaccgggtg gaatatcatg tatctgcatg tcgaaacacg tgaccgcgtt cagcccggta
     4261 catacgttca aactggtgac aaaatcgggc atcccagttg cgaaggcggc atatccaccg
     4321 gaacccatgt gcacgtggcg cgcaaattta atggagagtg gatcctggcg gacggtccgc
     4381 tgccctttac gttaagcggt tacgtggcga aaaacggcgg ctccccttac cagggcagtc
     4441 ttgaaagcgg gcaggcaagg atcatcgcca atccattagg aacgcttcgg tcttacatct
     4501 cccttcccta gcgcaatatc ctcccatctg gtataaaatt taccggtatg aaatcaacag
     4561 gaagtcgcat aaataaccac cgcggcggtt tcggttcgct gttcagcgcg atcatgagta
     4621 tcctgatcct gtctgcctgt tcgccgcagc ctcccataac agtgtacatt actccgtttc
     4681 cgctaagtgc caccgaaccc ccggctcagc agcccaccgc gctggccacg cgcccggttt
     4741 atcaacccgg cacgctggtg gcgtataccg cgcgtacggg tgacaacctg ccggcgctgg
     4801 cgcaccattt caacacaacg gtgaaggaaa tccttgaaaa caaccctcaa ctctctgaaa
     4861 aaacgaccac gctcactccc ggcgagaacc tgcagattcc gatttattac gaagcgcttt
     4921 gggggaactc cttccagata attcctgact ctctctacat caatggtcca gcccaaattg
     4981 gctttgatac caagtcattc gtcaacgctc atcccggctg gttgaagaac tacgcctcgc
     5041 tcgccggcgg cgaagtgcgc cgtggcgctg acctgattga ttacgtctcg ctcgaatatt
     5101 ccatcagccc gcgcctgcta ttggccttgg ccgagtacca ggcaggagcg ctaagcgtag
     5161 aaacccttga accgcaagaa aaagtttatc cgctcggcta tgaagatgaa ttccaccagg
     5221 gtttttacct tcaacttctg tgggcagcca acactttaaa taacggctac tacagctggc
     5281 gctccggcaa gctgggcacc atcaccaggc tggatggctc cattgaacac cctgacccct
     5341 ggcagaacgc cgcgacggtg gcactgcaga attacttctc acaggtgctt cccgttgccg
     5401 attatcacaa agccatttac agcgaagggt tatacaatac atacgctgcg ctgtttggcg
     5461 acccctggca acacacctct gaccacattc ccggtgattt acagcagcca gcgctgaggt
     5521 tgccattcgt gcctggaaag acctggtctt acaccggcgg accgcactcc gcctggggtg
     5581 aaggcgcacc attggctgcc cttgactttg cccctccatc tgtcgtcggc ggctgcgccg
     5641 ccagcaacga gtttgtcacc gctatggcgg atggtcagat cgtgcgttct gaaaaaggcc
     5701 tggctatgct ggacctcgac ggcgacggcg atgagcatac cggctgggtg atcctttacc
     5761 tccacctcgg gctgaacgat aagattcgcc ccggtatgct ggtgaaaaca ggtgacctca
     5821 tcgggcaccc ttcatgcgaa ggcggttccg ccaccggcac acatgtgcat atcgcccgta
     5881 aatacaatgg cgaatggatt gaagcggata gcgtggtgcc attcaacctg gaagggtgga
     5941 tcgcgaaaaa cggggtggtc ccttaccagg gaagcctgag caattatggg cgtatcatca
     6001 ccgctagcga caagggcctg gtaaattcct atatcaccgc ggggaatcca taatgcacga
     6061 acgaacccgt tctggtcaga ttcggctgga atcgatacgc ttgattttcg gcttgttact
     6121 gctagctatg gtcaccagcg gctgcgtggc caaccctgtg cctgatgagc ccgcgcctgg
     6181 ttccgggcaa ccaactgccg ccagtacact ggaaagcaac acgttggaaa cgtcggacgc
     6241 gatcaccgcg gcggttactg caaccgctga cttgccagcc acggattcac aaacgccgcc
     6301 cccccaggcg gaagctgaag cagactggct ctccatgccc atcgtgccga cagtatcccc
     6361 ccggatgaaa gacgtttttg aacgcggaca gaaaagcggt cgcgatgccg cgcgtttttc
     6421 aaaaattggc gattgtcaaa acatcacgac ctacttcctg gcgatgtatg acagcggtaa
     6481 ttaccggctg ggagatcaat atgcttacct tcagccaacg attgatcatt ttaaaggctc
     6541 ctggtggcgc cagagtctct cagtgaaagg cgggatgaac gtagcagcgg tgctcagccc
     6601 gatttgggcg aacccggata agtgcctgcc caaagaaact cccctggcct gtgagctgcg
     6661 cgtgtacaac ccggcgtttg cggtcatcag cctggaagaa tcctggtcag gcagtattga
     6721 gcattacgac atgtatctgc gcgaaatcgt ggaatacgtg ctggcgcagg acatcgttcc
     6781 catcctcgcc acgcgcgcgg aaactgaaac ccaggaaagg caaatcaacc ctaccgtggc
     6841 gcgcatcgcg catgattacc aggtgccttt gtggaacttc ggcgccgcag ccagagctct
     6901 acctaataat gggattcgac cggacgggtt ccatattacc gaggggcaat cttactttga
     6961 tgacgaggcc atgttaaaaa caggctggac ccaacgcaac ctcaccgctc tgcaggcgat
     7021 cgatgccgtc tatcgtggac tgacccagga gccataacct tacgcggttt ttaaggtaag
     7081 gtgcgcaagc ctgttttccc aggcaggtta ggcttgttca atcgtatatt tggtttgaat
     7141 attggctgcc tttcgcagca tcgccatacc ggggcaatat ttgttttcac ttaaatcgac
     7201 tgcccgcgcc accgcgtcgg gatcaatatt ccgtcccttg atgatatatt caatggtgat
     7261 atccgtgaac accttggggt gttcgcccgc gcggtcagcg tgcagcttca cctcgaaatc
     7321 agttacctgc tggcgtttct tttcaaggat tgagatgaca tccatcgccg tgcagcctcc
     7381 caatcccatt aaaatcagct ccatcggtac cacgccatct cccgctccgc cgtgatcaac
     7441 cgaagctcca agcggcactt caaaatttga atccgcgact cctttgaaag acaacccgta
     7501 tttccatctc agctttgcat ccattcttgc ttcctcctgg ttatgattgt gataattcag
     7561 ttctcatcaa ctttacaaaa cgaacccaac tctccaaact ggagaaggat ttctgttatc
     7621 aagtgaaact gccaccgggt tctttaaacc cgcacaccgc gcagcctaac accagccaac
     7681 cgtcatagtc taaaacgccc tttccacata ccgggcacgg ctgaccaggt ttaccagtaa
     7741 gacagcgctc gcctttaagc gggggtgccg cagccggggc atttatgcta tacgggcgtt
     7801 tcccgggttg ctgctggttc atcccgctgg tttcactcac caggtttctt ccctgttgat
     7861 tgagcgattt gcaatctggc gaacaggcgc gagcgcaccc cttctggcag cgtctcttcg
     7921 ccttctgcaa cctggtacag gtcgatcgtc ttttgcagcg tattcattac cacctggcan
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna attctgattt ccgcctgcgc
     8101 agcatcatta acgcttcatt cgtcgcgatc cggtaaagcc aggtataaag cgaggaacgc
     8161 ttttcaaacc ctcgcagcgc gttgtgcgct ttgagaaagg tttcctgcag catatcctcc
     8221 gcgtcttgcg cgtttcccag catgcgtaac cccagcccat aaatgcctgc ggaagtctgg
     8281 tcgaccagtt cattgaaagc ctgcccatct cccgcaatca aacggtcaac aaactcagaa
     8341 gggaactgtc tcgctgcact catatagatg atctttcaaa gaaaaagtga cttaactcaa
     8401 cggccagttt ggtagcacat ccagatccag cgggtctacg tgtcccaaac aaaaatggta
     8461 ataacccgcg ctggcaatca tcgcggcgtt gtcagtgcaa agtgaaagcg gtgggatatg
     8521 caccggaaat tcagtttgcg aggtgaaggt cttgcgtaaa gcttcgttcg ccgaaacacc
     8581 cccagcgacc aggatggact tcacggcgta atcacgcgcc gccttcattg ttttttcgaa
     8641 cagcacgctc accaccgctt cttgaaagct ggctgccagg tttggcaccg gcaaagaacg
     8701 gttttcctct tccagcgcgc ggacttcgcg taatacagcg gttttcaacc cgctgaacga
     8761 aaaattccat gtgttttcaa gccttgaacg cgggaaggga aacgcctggg gattgccttc
     8821 tcgggcagcc ttttggatgg ccgggccgcc tggataaggc aaccttagca tgcgcgcgac
     8881 cttgtcaaag gcttcgccag ccgcatcatc cagtgtgccg ccaaggcgct ggtattgaag
     8941 atggtcttgt accaggttga gttcgctatg cccgccagaa acgagcaacg ccaattgcgg
     9001 gaactccggc ggtggaggtg gttttggcgt atccgcaggg tagagccacg cggaataaat
     9061 atgcccttcc aggtgattca cgccaatcaa tggcagctgt ctgcccagcg ccaatccctt
     9121 ggcagcattc atccccacca ccagcgatcc tgccaggccg ggtcccctgg taacggcaat
     9181 ggcgtcgata tccgccaggc caaggttggc cttttgcaag gcttcatcga tcacagggta
     9241 aatggtgcgg atatgctggc gcgatgcgac ttcaggaaat acaccgccat atttggtgtg
     9301 caggtcgatt tgagtggcga ccacattgga aagtatctct cgtccgttct caaccaccgc
     9361 tgcggcggtt tcatcacagc tggtctcaat acccagtaag cgggctgatt taagtttcac
     9421 tgaacacgcc ctttctgcac gtcacctcat ttcattggca gcagcaggtc tttagggaat
     9481 tccgccagta tttcgaattc gccatccggg gtcacccacc acgaatcttc gatgcgcaca
     9541 cccataccgc gttcagggta atacaaccct ggttcaagcg taaacacaga cccgggagcc
     9601 aatatatctg ttggtgaagg gttgaccgca gatgcccagg gcttttcatg cactctcaag
     9661 ccgatgccgt ggcccaggct gtgcacatac ccccgttcca cagtcggatc cgactggata
     9721 gtcgggtgcc ccatctcctc gaacaacgcg caggcacgtt cttgaaattt ataaaaaggt
     9781 gcgttcactt ttaactcggc tgagatggta tcgtaaacct gcctgacctg gtcatagagc
     9841 tgctgcgctt catcagtcac gtagcccagc gaccaggtgc gggtaaagtc ataatagtaa
     9901 cctccgccag cctcacaggg atagatatcg tagacgatgg tctgccccag ttgcagcgcg
     9961 tcgctcgggt ttcccaccga atgggggata cccgcgtccc tgccaatagc aaaaatggtc
    10021 ccctcggggt tctccgcccc ccgctctgcc agccacagtt gaatgaggtt ctttacctcg
    10081 ccaatggtca ccggcgtacc atctttgtgt accaccacac caccctttgt tttatgcccg
    10141 gtgatgtaat cagcggtctt cgccaccaca tcggtggtca ccctccccat ggcgcggata
    10201 tgttcgattt cacccgcgtc tttggtcatc atggcgttca tcaagatctc gtcccgcaca
    10261 aagccagcga actgtagttc aggcatcaat tcctgtaaac gattcaggat tgaataaaaa
    10321 ggaccgatat cgtcttttcc ataaagagcc actcggccgc gggtgagctt catttcttca
    10381 agcattaatt taagccgtaa aacaaacgcc tccaggtaat tgccccctgc ctgcttgagc
    10441 aggtcgtgaa gagggtattt ggtcatactc acaagcttta atcctgattt ggcggcctct
    10501 tcgcgttcca tcgggtaatg aaaaagcacc gcatcctttc cccgctgttt gatcaatatc
    10561 gcgtcagaaa catgcgcggt tcccacgaaa tagaccatcg atgggttgtg ctgcgctgca
    10621 ccaataatga agattgcgtc aacattgtgc tttttcatca atttatcgag atctgttttc
    10681 atctgtgcgc tcctgtgata tgggttctga gtatcctgac cagatcctca tcttcttccg
    10741 gcaaaacggt acgcgggtca aggtaaagcc tgccgccctg caccctggcg atcaacggcg
    10801 gggtagactg gcgtaaccgc gcagccattt gttccaaccc tggtatgtta aaagatagcg
    10861 cgaaggtggg caaggtttcc tctggcaggc ttcctccccc cacggtggag tgttcttcaa
    10921 cgacctcccc acccacttgc tgctgccagc gtgtcgcccg ttccttcaag tcattcgcgc
    10981 tggctgaaat catgcgccag atggggatct ccttctccgc ttcgcctttc agataatgca
    11041 gcaaggtggc ttccaaccct gccaggcaca atttatccgc ccggacggca cgcgccaatg
    11101 gatgtcgttt aattttggca accaggtgcg ctttgcctaa aatgatgccc gcctgcggtc
    11161 ctcctaacag cttgtctcct gaaaaacaca ccaggtctac cccggctgcc agtgactcct
    11221 gcaccatcgg ttcatgcgcc aacccgaacc tggatgtatc cagcagtgcc cctgaaccca
    11281 ggtcatccag catgggcacc tcgtactggt gggcaagggc agtcagttct ttaagttcag
    11341 gctgtgaggt aaaccccacc agtttaaaat ttgaagtgtg cgcgcgcatc aacaacgctg
    11401 cgccgtcacg caacgcgctt tcatagtccc ccaggttgac ccggttggtg gtgccaattt
    11461 caaccagtcg ggcgcctgac tgcttcatca cttccgggat gcggaatcct ccgccaattt
    11521 caaccagctg cgaacgcgag ataaccaccg cccgcctgct ggccagggca gataaacaca
    11581 acaaaaccgc ggcggcgttg ttgtttacca ccagcgcgtc ctccgctccg gtaagccggc
    11641 gcaacagccc gctggcgtgc acgctccggg agccgcgttt tcctttctcc aggtcatact
    11701 ccagggtcgc atatcccccg gcgatctcgc tcatcgcttg ctgcgcgctg cggctaagcg
    11761 gagcgcgacc caggttggtg tgtaaaacca ccccgctggc attgatcacc cgggtaagcg
    11821 tgggagtgag cgcttgtatc aactggtttt taacccgttc caaaatggct tcctcggttg
    11881 gttcaaaggt ttctgcctgc ggattctggc gtatgctttc gagcacattc tggatgttcg
    11941 tggtaaccag tgatcgtcca tagcaatcca gcatcggggc agcgtgcgcg tgcagcaaga
    12001 gcctgtcaac agagggcagt tttcgcagat cgatcatggt ttcattcgag aagggaagcg
    12061 gttcagttcg cgcatacgaa tgagtaactc aatcgcaatg aacccgattg cgatgaagat
    12121 cgccagcgca aaattcaaca ctctgccaaa ttgcatccat agcagaatat ctgcgtaaat
    12181 gccccaccag gcggcttcgc ggatgatcac cttggaggtt acaggcgggt tggaaggaaa
    12241 tcgcagatgc aggtaaaaaa tcgccggaag cgccaacccg cttgccagca gagtgacgaa
    12301 aaagaacaac aaccacctgg ggccaagcgt cggcacggtg aaaaggaaca aacccatcaa
    12361 cccgactgtc gaggtcagca ccaggatgat gctggtggtg agcaccggca taaaaagcat
    12421 tcttggaggt tgagtgtctt tcatgcgcag atgaggttcc ttttcaatta ttgataatcc
    12481 attatatcaa ttgcgccggt taatgaacaa cttctgcgcg aaaaaaccta atgcctgttg
    12541 ttgttcaggc tcttgacctg gaagcgcatt tgattcatgg taaaatctag ccattacagg
    12601 ggattagtga cccgctcatc aagagaggtg gagggaccgg cccgctgaaa cctcggcaac
    12661 cgctcacgca gactggtgcc aactccggca gaagggattc tgaaagatga gagagaaagc
    12721 accaccttca aacaacctct ctcgcgagag gttgttttta tcagtaaatc gctcaaggag
    12781 accataatgg atcgtacatt caccaaccgt caatacctgg acgcgctgca aagcaaggta
    12841 atggtgtttg atggagcaat gggcaccaac ctgcaagccc agaatttaac tgccgaccat
    12901 ttcggcggcg aaaagaccaa cggctgcaac gattacctgg tcatcactta cccccaggcg
    12961 gttgaaaaag ttcatcgctc tttcctctca gtcggcgtgg atgtgattga gacctgtacc
    13021 ttccgttcaa accgtttaac cttaagtgaa tacgggttat cacaagaggt gaccgccatc
    13081 aaccaggctg cggctgcttt ggcacggcgg ctggcagacg aatattctca aaccgggcaa
    13141 ccacgctatg tagccggatc catcggtccc tctggcaaac tgccctctat gagcgacgct
    13201 gaactatcca atatcacttt cgatgagctc acctcaatct tcaagcagca ggcagaagga
    13261 ttgatcctcg gtggggttga tttgatctta attgaaacct cgcaggatat cctcgaggtc
    13321 aaagccgcca tccatggtat ccagcaggct ttttccagca gcggggtatt tctgccgatt
    13381 caagcccagg tgaccctgga tacaaccggg cgtatgctgc tgggaacgga catcacggct
    13441 gtgttggcga tactggaagg cctgcccatc gatgtgatcg gattaaactg ctccaccgga
    13501 ccggatttta tgcgtgaacc cattcgcttc ttgggcgagc actccaccct gccggtttcc
    13561 tgcattccaa acgctggttt gcctttgaac gtgaacggtc aggcggttta tccgctggaa
    13621 ccggagccgt tcgctcaggc gctttataat ttcgtggtca accatggcgt cagtgtggtc
    13681 ggagggtgct gcggtacaac agcagcgcat ctcgcccgcc tggtagaaaa agttcgcgca
    13741 atccagcccg gccctcgact tcgtatttcc agcgcgcggc tctcctcagc catgcaggcg
    13801 cagccgatgc aacaggaacc accgcctttt ttcatcgcgg aacgattgaa cacacagggc
    13861 agccacgtat ttaaaaatat ggtgctggag aatgatttcg aaagtatcct taccctggct
    13921 caacaacaaa tggagagcgg cgcccacggg cttgacctgt gcgtggcatt gactgaacga
    13981 gccgacgaaa aagaaaccat gcagcggctg atcaagacca tcgctccggt catcaatctt
    14041 ccgctggtca tcgataccac cgaagtggac gtgctggaaa cagcgcttaa aacagcgcca
    14101 ggacgctgcc ttatcaactc aacccacctg gaaggcgggc gtcaaaaagc tgaccgcatc
    14161 ttcgggttgg cgaaaacaca taacgcggcg gtgatcgtgc tcacgattga tgagcagggc
    14221 atggcgaaaa ccagagagcg caaactggag gtcgccaggc gcatctacga tatcgctgtt
    14281 aaggaacacg ggctggcccc tcacgacctg gtttttgatg atctcacctt caccctcgcg
    14341 actggagcag atgagttcac caactcagcg attgaaaccc ttgagggcat ccgcctgatc
    14401 aaggctgcgc tgcccggtgt gctgacctct cttggcgtca gcaatgtgtc tttcgggctg
    14461 gcagcgaatg cgcgtaaggt catcaacagc gtgatgcttt accactgcgt tcaggcggga
    14521 ttggatatgg ccattgtaaa cccggcgacc atcacgcctt accccgacct cagcccagaa
    14581 gaacgggagc tcgctgaaga tttgatcttc aaccgcaaac ctgaagcgct tcagcgcctg
    14641 attgaattct tccaggccgc gggccccagc agcgcccagc aaaattcaca gcaagaagcg
    14701 ctttccatga tgacacctgg cgaacgtctt cactggaaga tcgtgcaccg cgcgaaagaa
    14761 gacctcgaga ccgacattga caccctgctg cagatgaggg aaaaggaggt cagccgcaac
    14821 gacgccgcgg taggcatctt gaaccatgtt ctgcttccag cgatgaagga tgtgggtgat
    14881 aagtttggca ggggcgagct gatcctgccg tttgtgctgc aatccgccga ggtgatgaaa
    14941 aaggcggttt cctatttaga gaacttcctt gaacgaaagg aaggtgccag taaaggtaag
    15001 ctcctcctgg cgacggttta cggcgatgtg catgatattg gcaaaaacct ggtcaagacc
    15061 attctctcca acaatggcta taacgtggtc gacctcggaa aacaggtacc ggcggatacc
    15121 atcatcaacc gtgcagttga agaagaagcg gacgccattg gtttgagcgc gctgctggtc
    15181 agcaccagta aacagatgcc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnctggagcg ggccgcggcg gaggtcaagc caatcagcgt gcaggcggag aaaaccgttg
    15361 agcccgccgc gctcatcccc tctccaccgg gctatggtgt gagggttgtg gaggatatgc
    15421 ccctggaaat cgtttttaaa cacctggcgc tcaacgaact gttccgttta tcctggggag
    15481 cgaagaacgc gcatggcgcg gaatgggaaa agataaaaac ggaatacgaa aagcgtctgg
    15541 ccctgatgca gaaacaggcg atcgctgaga aatggctgaa acccagggga gtgtacggtt
    15601 actggccagc gcaatcggat gggaatgacc tcctcattta tgatggagat tctgtggaaa
    15661 agggaaaccc cattgtgatc aaccgcttca ctttcccccg gcagtccagc ggagagttcc
    15721 tctgccttgc agattatttc gccagcgtgg attctggcag gatggacgtg gttgctttcc
    15781 aggttgtgac cgtcggcaaa gaagccagtg aacgtttcga ccgtctgcag gctgtagcag
    15841 actatgccga agcctacttt tcgcatgggt tatcggtgca gaccgcagaa gcgaccgccg
    15901 agtacctcca ccgccatatc cgccatgaac ttgggctcag cgaaaaccag ggcaagcgtt
    15961 attcctgggg gtatccggcc attcccgagc tggaagatca caagaaggtg tttgccttat
    16021 tgcccgcgga agcagccctg ggcatgagcc tgacctccgc ctgccagctg gttccagaac
    16081 aatccaccgc ggctattatc ctccatcacc cccaggcgaa gtattacagc cttggtgaaa
    16141 cccgcgtaga acaattgatg aagtaaacgg tgtcgaatga tcaatcaagc aacccttctg
    16201 aacctcctgg caaaaaaacg cgccctgttt tctgacggcg ccatgggcac catgctgagc
    16261 cagaacctgc ccaccggctt aacctgtctg gattacgcca acgtggaata ccccagggtg
    16321 gtcgaatcga tccaccgtga atacatcctg gccggttctg acatgataca gaccaacact
    16381 ttcggcgcga acaggtacaa attggcccaa caccacttgg agaaccagct ggaacagatc
    16441 aacaccgcgg gcgcgcagat tgcccgccaa gcggtgtctg ccagcggcag ggaggtgctg
    16501 gtcgctggtg atatcggccc actgggggtt cgcattgcgc cttttggtag ggttcaactg
    16561 ccagaagccc acgaagcctt ttcacagcaa gccacggcgt taatcaaagc cggcgtcgac
    16621 ctgatcatca ttgaaacgat gaccgacctt tacgaaactg ttgaagcact gcgggcggtg
    16681 cgtgaaattt caccgcagtt accagtcatc gcctcgatga ccttcacccg cgataaccgc
    16741 actctgctgg gcgatccccc caccagggtg tcgcaacagt tacacgctgc cggcgcggat
    16801 gtgatcggcg tcaactgctc tggcggtccc aaccagattc tcaacattct caggcagatg
    16861 cgccgcgctg tgccggatgc gattttttca gtgatgccca acgccggttg gcctgaacag
    16921 gtggagggtc gcatcatgta ccccgccgct cccgagtatt tcggcactta cgcgctctcc
    16981 ttctggcagt ccggcgccag catcattggc ggctgctgcg gcaccactcc tgcccacatc
    17041 gccgccatgg tacaggcttt tcaacaatcg ccaggattgg aacgctcagc aatcgaagag
    17101 atcgcgctat ccgcaaccag gcagccggaa gatgaaggcg ccctgcctcc cacaaaactg
    17161 gcgcaaaaac tccgcgatgg gcgattcgtc atcgctgttg aaatggatcc accccgcggg
    17221 ctttccaccc acaagttaat tgccggcgcg agcctgctgt ctgaagtcgg cgcggatgtc
    17281 atcgatgtgg cagacagccc catggcgcgc atgcgcatga gcccctgggc ggtttgcagc
    17341 ctgatccagc gcaatgtggg gatcgagacc gtgttgcact ttccaacgcg cgggcgcaat
    17401 gtgctgcgcg tgcagggtga cctgctggca gcccacgcgc tggatgtgcg caatattttc
    17461 gtggtgatgg gtgatccgac ttctattggt gagtaccctg acgcgatgga tcattatgat
    17521 ctggttcctt ccgggttgat caaactggtc aaacaggggt tcaacgccgg tgtggaccat
    17581 gccggaagcg gtattgggca gccgaccagc ttttttgtcg gctgtgcgct taacctgaaa
    17641 gccgcggata tggaagcaga actgaagaat ctgcgccgaa aactgaaagc cggagcggat
    17701 tttatcctca cccagccgat ctatcacccc gctttggtaa ctgatttttt gcagcaatac
    17761 gagtctcagt ttgataaact acccgttcca ttaattgtgg ggctgctgcc gctggccagc
    17821 gcgcgccacg ccgctttcct gcaggcggaa gttcccggaa tcgaaattcc gcaaagcatc
    17881 cgtgacctga tggcagtcag cggtgaccag ggagcgcaga ccggtatccg ccttaccatc
    17941 gaactcatcc agaatatcaa accaatggtg cagggcatct acctgatgcc cgcattccaa
    18001 cgctacgact acgccgccga gatcatcgaa aaaacaagaa cggctgaata atgtaaaata
    18061 aacccattga accatcaaaa aaccgaccaa gggaaaaaat gaaaatcctg gtgatttctg
    18121 atgtgcacgc caacctgact gcgcttgaag ccgtcctcgc ggatgccggc aataattatg
    18181 aggttgtgtg gtgcctggga gacctggttg gttacggacc agacggcaac caatgcgtac
    18241 agcggatacg cgagttgccc aacctgcttt gcatcagggg caatcacgac ctcgctatct
    18301 gcggtgacgc tgacctgagc atcttcaatc acgaagccgg agaagcgatt atcatttccc
    18361 ggcgcatgat gtcgccggaa acgctgcaat acctgcgcag tctgcctgaa aaacaggtgt
    18421 caggtcaggc aacgcttatc catggcagcc cgcgaaaccc ggtttgggaa tacattttag
    18481 atgtcagcat tgcggctgag aattttgcca gttttgatac acaactcgcc ttcgtcggac
    18541 actctcacct gccgctgatg ttcactgctg accctcaaag tgggcttgtc gaacgcgctt
    18601 accgataccc cggcgttacc acgcctgtta ccacccgcgc aatcctcaac cccggatcgg
    18661 tcggtcaacc acgggataat gaccccagag cgtcttacgg tatttttgat cctcagaaca
    18721 tgacctggga aatccatcgc gttccttatg atatcccctc tgtacaacaa cgaatcatcg
    18781 ccgctggttt accggaaaag catgcccgtc gcctgagcgc tggctggtga gcaggaacca
    18841 atcttgctcc tctggcgtct attaaacagt tgcacacatc ggtttatttc aaggaggaat
    18901 ggtatgaaaa cgaaaagcgt gttgattggc atcgtcattc tgtcgctcac cctcagtggt
    18961 tgcgcgtcca aatcctacac tgctaacaat ctgcggtcag gcggggtgga agattacgcc
    19021 atgaaagcag cccccatggt agaacccatg gagcccatgc tggatagtta cgcagaatct
    19081 gccggcagcc ctggtatcgc agccgcaggg gtggaatccg tgcagatgat catgatgaac
    19141 gccagtctgt cgatcgcggt tgatgaccct gccgccaccc tgcgtgacgt gcagcagctc
    19201 gccaaaggta tgggtggtta cacagtttct tccaacctgt ataaaagcta tacgtcttct
    19261 ggggtcgaag tacctcaagc caacctcatt atcagagtgc ccgctgagcg cctggacgaa
    19321 gccatgcaga agatcaagga catgactggt gaccccaaaa aatatacttt gaatgagagt
    19381 gtctccgggc aggatgtgac caaggaatac accgacctga aatcacgttt acgcaacctg
    19441 gaagaagccg atgccaaatt gagcgagttt tacgaaaacg ccgccaacac agaagatgcc
    19501 ctttccatct attcgcagaa aatgcaggtg accgaacaaa ttgaaataat caaagggcag
    19561 atcaagtatt acgatgaatc ggtttctacc tcgtccatca cattgcagat tcaatccaaa
    19621 gaaactatcg ctcctatcac ggtggctggt tggcagcctt caggggttgc gcgcgacgcg
    19681 cttcaggcgc tgctggattt cggcaaaggt ttggtcaact tcctgatctg gctggtgatc
    19741 ctggtggttc ccattgtcct gttatttggc ctccccatct tcttcctggt gcgctggttg
    19801 aaacgccgca gcacagcgcg caagctggca gcgctggaac gccgcgcgca agagaataaa
    19861 tcagaaccaa ccccaccgat cccgacgaaa aagtgaatcc acgtccacaa ataggcaagg
    19921 agcggtcaac ccgctccttt ttctatctca taatacgcac attgcaaaaa ccttccacga
    19981 cgactggcag acgataaatt ccttgctcca tagggtgcaa ataaactggc ttgccgtcca
    20041 caaacgctgc ctgtggcttg cctggcaggt ggaaaaacac ctgccctatt gccgtccttg
    20101 gcaggcgcag cgtcatctcc aggctgccgt ccttttcgag ccactcagcc acctcagcgc
    20161 cttgagtgaa atgcaggtcg ctgcccaggt acagcgcttt ttcgcccaca aacctgcgga
    20221 cagccagtaa aactggcgcg tgcggcggta tgcctggaaa tacgtgcccg cctgtcccgg
    20281 aaatcatgcc tgtcttcccg ctccaaaact cgcggaatag atattccccc tcttcaagcc
    20341 catactcgct ggctgaaatc attacatccg cggtctgttc agaccagttg aacccgccca
    20401 gcagatgcca gccgccagtc tcattgtcca tatctacacg aagtctggac ggcgtggcgt
    20461 tgtcgaacca atccaagaca cgcacttctt cacccataac tggaattaac gcttctgcca
    20521 accgtatacg ctcgggtggc actccaggca ggtcatctga aagcagcaat gagccgccag
    20581 tgagtgcgat gaccgttgcc agtgaacgca cctcttcctg cgtcaggctc gtgtcagcgc
    20641 gcacaagcag gcagtctggg tcatttaccc accagcgccg gttgaggttg gcccgggtaa
    20701 ggatattgtg aatggaattg cgcgtgctgg gcatcgaggg ttcccggcgg aggcctatct
    20761 gcacaccttt gaagaccggc agccactcct cgctgacatc tggcccaatg cgcatggtat
    20821 cgaccacgcc gatgaccgaa cccaacggtg ctccgcagcc aagcagggtc gtttgttcgc
    20881 cagctgcctg ccgcaacgct tccatgccca tgcgcatcac ctgcgccctg gtcttactgc
    20941 ggtcgtagta gtgacccttc agcgcggcag catagagaaa gtccagcttc aaatacggga
    21001 acccccattt ctgcactgcc gtatgaacca cctccgcggc atactgtaaa gccggcggtt
    21061 tggtcaggtc caaggcggtg gttaacgcgt tccagcctaa ccctgcattc accagcctgc
    21121 cgttggcttt gcgcagcatc caatccgggt gtttatgcag caggtcagct ccagggtgca
    21181 cgatgaaagg cgccagccac aatcccggca gcagtccacc gccactgatt gatgcagcca
    21241 gcggggctac cccctctgga aaggtcgatc tgaactccag ccagtctcca acctgttttt
    21301 caaacccgtc atcgatctgc accagttcaa tgggcagctc ctcctggcgc tcaaggatcg
    21361 tgtccaggtt ctcgataata ttcctggcgg tcaccgatgt gtaaaaatga taccaggagc
    21421 accaccccac cggggtttgc gcgggcacgc gcgcgtggtt ttcccgccct accgcttcga
    21481 tataagtctc cagtggggcg cgatgatcca gcaaaacagg cgtgaacacc gcccaatccg
    21541 tttgcattga cttccccgct tcaaggcagg caccatctcc attcgcccac atggagattt
    21601 caccggtatt gaagtcagcc aggatcgagc caaagtggtt caactgagaa aggaaaccga
    21661 gaaggtaacc agcacgggtg gcgcggtctc cgaccaccgc gaacatatca ctggagaaaa
    21721 cacctttttt aacgggcgtt ggcgttcccg ggttataaat catcgggcgc tgcaatgcgt
    21781 acagccggct gatggtcatt ctttgattcc cactgtacca tcggctggga gaccaggatt
    21841 gccagccatt ggagaaaaaa cccaggtcat ccggccgtct gaccttcagg taatgggtgt
    21901 tatccaccac gcgcagcagg gtgatgcggt ttatctccac accttctttc ccgtggttgg
    21961 tgacctgaat cttccacaat accagcgggt attctcgcat aagcgcgaaa gacaggcgca
    22021 ggctgacctt gttggcatcc ggctcaagcg caactgtcag gcaatccatt tcgccatgcg
    22081 cctgcgcgcg ctccaggtga tgcgccaagc cattcacctg ccagccatct tgaccgcagc
    22141 gctgctgctt ccccgcgaac gtgtactcgc agcccagttg aatattctcc aagcggggaa
    22201 aacgagggtc cctggcctgg atcgagaaag tccctgaatc agggttgacc tgaagcatta
    22261 atgaaacaga attgagctgc tccaattaaa tcctccacac aggcaatgaa taaagtaatc
    22321 ttatcacgag gctctaaaac ccgcttccag tccccttaaa acacgcgtga tataattgcc
    22381 gcatgccccc catggtcgcc attgatatcg aaacaaccgg aatggacgcg cagcgtgacg
    22441 cgatcattga aattgccgct gtgcgtttta atgggcacag ggtggaagga gaatggacga
    22501 agctgatcaa ccccggccgc cccattcccc ggcaaatcac gcaactcacc ggcatcactg
    22561 accaaatggt gctgcacgcg ccaccactgc aagcggtcat tcaggaactg gtggattttg
    22621 taggcaacga catcgtggtc ggtcacaatg tgcgtttcga cctgagtttt ttacaaaggc
    22681 agaaggtcct cctttacaac aaagtgatcg acacctacga gatggccgct attgcgctgc
    22741 ccggtaacag ccgttacaac ctcggcaccc tcggggttac ctgcaacatt ctgattccca
    22801 actcgcaccg cgccctggac gatgcccggc tggcgcaggc agtctattat tcactctcac
    22861 aaaagttgct ggatttaccc atcgaaatcc ttgatgagat cgtgcgcatg tcagaaggca
    22921 ttgaatggga tggcgaggtt gcttttcacc tggctctgca ggcaaaaggc cgtttaccca
    22981 ttgaagcccg gaaggcttct caagacgatt tcggcttgct ttttgcagga acgcagagct
    23041 ttcctccgtt ggttccagtc gaaacacctg tgcggataga ccctgatgag atcgcggcgc
    23101 gaattgaaca cggcggacca ttttcgcgct acttcgataa tttcgagagc cgcccccaac
    23161 agcttgaaat gctgcgatca gtggcgaacg cgcttaataa cagccagcat ttcatcgtcg
    23221 aagccggtac cggaatcggt aaatcctttg cctatctcat ccctgccgtg gaatgggctg
    23281 tgcgcaattc aatgcgcgtg gtgatttcga ccaataccat cactttacaa gaccagctga
    23341 ttaaaaaaga tattcctgac ctggcgtcag cgttaggctc agaggtgcgc gccacagtcg
    23401 tcaaaggacg cggtaattat ctctgcccgc gccgcttcag cgcaatgcgt aagatcggac
    23461 cgcagaacgc cgaggagctg agaatactgg ctaaaacgct ggtctggctt taccagggcg
    23521 gcagcgggga ccgcggcgaa atcaatctaa atggtccgct ggagcgcgaa gtctggcagc
    23581 ggctttcagc cgaagatgaa ggctgtaaaa cagaagtgtg cgtctcccgt atgggcggcg
    23641 cctgtccttt ttatcgcact catctgacgg cgcaatccgc gcacatcctg gtagtgaacc
    23701 atgccctgct gttggcagat gtggtcactg gaaaccgtgt gctgccagaa tacaattacc
    23761 tgattgtaga tgaagcccat cacttagaat ccgccacgac caacgccctc tcctttcggt
    23821 tgacccaaaa cgattttacg cgcatgctgc gcgagattgg cggcatctcc agcggcttgc
    23881 tgggcagatt gatgagcctg gtttctcacg ccctgccgcc atctgatttt gctgcgctga
    23941 acatggcagt gcataaggtc tctgacctgc tcttccagtt ggaaaatgaa ttttctgaac
    24001 tgattttcag tttagacgca tttatggatg aacaacgcga agggcagccc gtcagcagtt
    24061 atggtcagca atgccgggtg ttgccggcta cgcgcacttt accctgctgg tctaatgttg
    24121 aaatcgcctg ggatagctgt caggatgccc tgggcgctgc gctaaaaatg atcgccgacc
    24181 tgcaaaaagc cattggcgat agcctggacg aagttccgca agaggtggag gatacactgg
    24241 gttcgttggc cagtgtgtac cgccgcttga gcgaagcgca gaattacctc gaggcgctgg
    24301 tgaaccatcc agacgccaac tatgtgtact gggtggaaat tatgcccaat acccagcgcc
    24361 tggcgctgca ggcagctccg ttgcatgtgg gtccgctcat ggagaaatac ctgtggcacg
    24421 ataaagcctc catcatcctc acctccgcta cgctcaccgc caataacgag tttgaataca
    24481 tccgcagccg cctcaacgcc gatgaagcgg atgaactggt gctgggttct ccgtttgatt
    24541 acgaaaactc caccctgctc tacctggtga atgacatccc cgaaccggct gaagccagcg
    24601 cgtatcaacg agaagtggag agcgccatca tccgcctggc taaagccacc ggcgggcgta
    24661 tgctggtgct gttcacctcg tacgctcaat tgagaaagac cgcccaggcg atcggacccg
    24721 cgatggcgga cgcggagata caggtctatg aacagggcga gggtgcttcc accaacatcc
    24781 tgctggaaaa cttccgtgga gccgataaag ccgttttgct gggtacgcgc gcattctggg
    24841 aaggcgtcga tattccgggc gaagcgcttt ctgtgctggt aatcgtaaaa ctgccctttg
    24901 atgtcccttc tgaccccatc atcgctgccc gcgcagaaac ctttgaagac ccattcaacg
    24961 agtacaacct gccagaagcc atcctgcgtt tcaggcaggg ctttggtcgt ttaatccgct
    25021 cccaatctga ccgcggcgtg gtggccattt tggataagcg agtgctcacc aagaagtatg
    25081 gtcgtatgtt cctcgattca ctcccggaat gtacccgcag ggtggcttcc agcctggaac
    25141 tacccgccgc ggctgcccgt tggttgaaca tctaaaccct ttttccggtg ttattgttac
    25201 acgaccacaa tccctttatt ggagcaatat gatgagatac gttttcagtt ccatgcgtcc
    25261 gcggtttctc ttgctcgccc tcgcctgcgt tttcctggga actgcggcag ccatctgggc
    25321 agttggtaag gtgaacccct ggtatgttct actggcgttc atcggcggtc tggcagccca
    25381 cgcctgcgtg aacgccttta acgattacaa tgatattcgc agcggtttgg attttcgcac
    25441 caaccgcacc ccattcagcg gtggcagcgg cactttcgtt gaagaacccg aaaaggtgca
    25501 cctggcgtta tggaccacca tcggcactgc gctgattacc gtggtgattg gcatttattt
    25561 cgcgctggcg gtcggctgga tcatcctgcc cattggcatc ctggggctga ttgtcatctt
    25621 aacctatacc caatggctca acaaacaacc cctcttatgc ctgctcgcgc cagggttcgg
    25681 ttttggcacc ctcatggtgt tgggaacctt ctacgtgcta acggggcgta ttacctggtc
    25741 ggcggtactt acatcttcta ttcctttttt cctggtcagc aacctgctcc tgttgaatca
    25801 attccctgat gtagaagcgg ataccacggt gggtagaaag cattacccca tcctcattgg
    25861 cagaaaaacc agcgccatca tctacggctg tttcttagca gccacgtatc ttgccatcat
    25921 cctgggtgtg atgctcagaa tcactccggc atggacgctg ctcggcttgc tgacagccgc
    25981 catcgcgttg ccaaccgcgc tgaaggtgct ccaacacgca gagaatatcc ccgctctggt
    26041 gccatcgatg attcaaaatg tgctgctcaa cctggtaacg ccgctgttga tgggtgttgg
    26101 tttctt
//
LOCUS       DAOSPM010000016        25736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1912_length_25736_cov_16.278766, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000016 DAOSPM010000000
VERSION     DAOSPM010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 25736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25736
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1912_length_25736_cov_16.278766"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..763)
                     /locus_tag="PLW25_02725"
     CDS             complement(245..763)
                     /locus_tag="PLW25_02725"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOS53433.1"
                     /translation="MSDQTKQWSTPPEMSIDPEKSYTATFKTDLGSIICSLHAKKTPR
                     TVNNFVFLAREGFYDNTIFHRVIPDFMAQGGDPTGTGMGGPGYKFKDEFASSLRHNAP
                     GILSMANAGPNTNGSQFFITHVPTPWLDNKHSVFGQVIKGLDVLLSIPARDPSQRNSP
                     AVKLLTVEIAEA"
     gene            complement(801..1661)
                     /locus_tag="PLW25_02730"
     CDS             complement(801..1661)
                     /locus_tag="PLW25_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOS53434.1"
                     /translation="MIDWLWTPLQYAFMVRGMLAAVIVGMVCAVLGTYVVLRGMAFFG
                     DALAHAVLPGVAVGYLLGGGARGPVFWWAMATALLSAFGIGAVSRAARIKEDTSIGII
                     FAGMFALGVALISMVRNYTADLTHFLFGDVLGVRDTDLLLTAVFGALIMLFILTFYKE
                     FLVISFDPTLAATLRLPIRWLDYLLIALIAMSIVVSLQTVGVTLMVAMLITPAAAASL
                     LTRRLSRMMLIAALIAIFSGISGLYLSYYLNLASGSAIVLTCTAVFAIIWGITALARR
                     YSRARATENM"
     gene            complement(1658..2500)
                     /locus_tag="PLW25_02735"
     CDS             complement(1658..2500)
                     /locus_tag="PLW25_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOS53435.1"
                     /translation="MNSVAQPTANTITPRSHPVHIPGEPILRVEHLSLAYEGVVALQN
                     VTFQLITGERMAVIGPNGAGKSTLFKIIAGLLKPGSGTVSVFGAEPSGHICISYLPQH
                     NHVDWNFPVTVGEVVMMGRTRKIGFFRWPSAGDREIVSRALRTVGLHAHAHRQISQLS
                     GGQQQRMFIARALAQEADLMLMDEPVTGLDTPSQEELFTVLDTLREEKVTVMFALHDL
                     KLAATCFDRVMLLNRRLVGIGKPADVFTREHLSKAYGDRAQVLENGHGLSMVDDTCCG
                     GREE"
     gene            complement(2506..3444)
                     /locus_tag="PLW25_02740"
     CDS             complement(2506..3444)
                     /locus_tag="PLW25_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOS53436.1"
                     /translation="MHKKKSLLLLLSFIILLASACTGQITPTAKQLPVVATTSILADV
                     VSRVGGNLVSVTTLVPPGVNEHEYQPSPRDIAAVSDAALVFEVGLGLEQFMDAIIRNA
                     SDDITPIEVSSGISTQELLIPHDANTAEQQHEHTTDPHVWLDPANVIVWTENIAAALS
                     ARDPAGSGTYQANAAAYIAELKALDEWIRSETARIPQEKRLIVTDHMLFNYFAEKYGF
                     TVVGALIPSYSSLAQPSAQEIAALEDAIRQYNVRVILVGNTVNPTLAARVAEDTGTAM
                     VQFYTGSLSNADGPAATYLDYMRYNVKTIVNALSGK"
     gene            complement(3437..3895)
                     /locus_tag="PLW25_02745"
     CDS             complement(3437..3895)
                     /locus_tag="PLW25_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOS53437.1"
                     /translation="MNKLEHWLKTLKEDGCRITSPRRVILEVLIDSPRALQPAEVHAL
                     CRQTHPGIGLVTVYRTLERLESLGLIQRVHREDGCHLLMPAADGHEHYLVCTTCRRTI
                     LFKGDNLAPLFTKVESRTGYQVSEHWLQLFGTCPDCQLNSQQVTPKVNHA"
     gene            complement(3993..4988)
                     /locus_tag="PLW25_02750"
     CDS             complement(3993..4988)
                     /locus_tag="PLW25_02750"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOS53438.1"
                     /translation="MSVNQKRVSLFLPVILIGAGVILLLRNLGFLPAFNWSVLLRLWP
                     LALVVLGLDLLFGYRAPWAGGLIGLLTVAAVAVFLFFSPVMENNPPAGIKTEVVSKPL
                     ENTAHVQYYLDTSYPPVTISALPDSTDLVKATIVHRGQVHFDVKGDTDKTVTLSTSTE
                     PGPWFSYDLGLGGQKWDIGLNPSVPTTLNLDGGSGALDLQLEGITLEALRADFGSGAS
                     KITLPDSPQPYQVDIESGSGSVELALPTDTDVTFTLESGSGAVTVFLPLTSAVKIEVM
                     DNGSGTVHVPAGYIAAAGNTADHGTWQSSNFSSAAHTILIRVKDRGSGNLFFKTR"
     gene            complement(5012..5758)
                     /locus_tag="PLW25_02755"
     CDS             complement(5012..5758)
                     /locus_tag="PLW25_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOS53439.1"
                     /translation="MRELSLPGRAKPYVMAHRGNRVLCPENTLSAFLKAFEEGADILE
                     TDLHLSADGVFMCIHDATVDRTTNGKGAVNSLTLDELKQLSASYNFKGFENERIPTLA
                     ETAGILPPGVALALELKTDRFLEEEVCRRLVAELRSSGILERSLMLSFSLPRLQAVKA
                     VDPSIPIGWITMSQLTPNPLPEVLGPFWPGVFLNPFFVRRAHQRGQLVCPLDPTPDRR
                     LWYYKLLNCDALVTDNPGATLEVLKRISFK"
     gene            complement(5751..7763)
                     /locus_tag="PLW25_02760"
     CDS             complement(5751..7763)
                     /locus_tag="PLW25_02760"
                     /inference="COORDINATES: protein motif:HMM:NF027792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5054 domain-containing protein"
                     /protein_id="HOS53440.1"
                     /translation="MERSVHLIFKTHLDVGFTDYAAVVVRNYFKKYIPAALRLARELR
                     QSGSDRFIWTTGSWLIYEYLEQASSAERGQMEEAILAGDIAWHALPFTTHTELMDAEL
                     FRFGLSLSRTLDQRFGRHTIAAKWTDVPGHTRAIIPLLAEAGVVFLHIGVNPGSTVPS
                     VPSLFRWQDPGGAELLVMYESGYGSVFEIPGLEHSLAFGHTMDNMGPQSHEQVLDVYR
                     EVRSRQPGARVFASTLSDFAAQLVRVRENLPVLSQEIGDTWIHGVGSDPIKVARFREL
                     LRLRAEWLASGRVTRSEPSLINLNRWLLMVPEHTWGMDEKTWLNDHENYTVKDFNLAR
                     GQPNFQRFEASWVEKRAYPQLAVNALGGGSLADEARARLESIRPRVPDLSAWQPIEPA
                     QAKLENPSLAVTFEQQKGSLDQLVDRTTGQSLVVRPGEFSVFSYQTFSSEDYERFFNT
                     YVLPQQRHGWALEDFCKPGLRPEDSPSRCWLPLVKGIFKRSTPGVEHILFYLTFEDEA
                     LHQFGAPRNGYLQYSLPSQEQRLHIDLQWFNKQASRMPEALWMTFRPEHHPDAEWSIE
                     KLGREIPALSVVENGNRHLHGSGRYVTCRALGSELRISALDSPLVALGARSLLDFNND
                     QPDTRQGAHFCLFNNLWGTNFPMWFEEDCRFRFDITLNPTKEERNA"
     gene            complement(7768..9246)
                     /locus_tag="PLW25_02765"
     CDS             complement(7768..9246)
                     /locus_tag="PLW25_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS53441.1"
                     /translation="MKRLLKWYELLFLNSYQLGLSIASNVISPLLLPALVLVFMPPEK
                     KNTSTALVYVTGLAVAMFIQPVMGMLSDRSTSKMGRRRPFIIVGALLNILFLGVMGAS
                     LWFKSSPLNAFLQSSFGITASLAVLLLGIILLQFSSNISQAGQQGLIPDIVPEQQRGR
                     ASGIKSVMEMVPAALVLLISPLIDRQKFWLVILILMLSFLVTMLITVAGTREEPLQEK
                     PPRAEDRPVLRMLLLTVIFVGITQLALLLVKGIGTWLPQDSTSLALRIVLVGLAGLVG
                     MAGSIFIGVYFGARVGIGKEANTHKSFIWWVVTRLMFLAAISSVRNFAMFFVKDVLQV
                     PNPATITTYLTAVIFIFLLISSIVGGILADKIGRKKLLAAAGALAVLGTIILVFSRTI
                     PMVMFAGVFLGLGTGTFMASNWALGTDLVPQKDAGRYLGISNLAGAGAGIIGSSIGGP
                     LADYFNAIQPGLGYPVIFALYGGLFLLSVLLLPRIQAETSLS"
     gene            9559..10176
                     /locus_tag="PLW25_02770"
     CDS             9559..10176
                     /locus_tag="PLW25_02770"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOS53442.1"
                     /translation="MNKVFELPQLKFAFDALEPAIDARTMEIHHGKHHAAYVANLNKA
                     LEASPEFFSMPIEDILKGINTVPETIRTAVRNNGGGHANHSLFWDVLNPGGKSAPSGA
                     LLKAIESAFGSTDAFMEKFTAAATTRFGSGWAWLVLDGQKQLQVYSTANQDSPYMDHH
                     IPILGLDVWEHAYYLNYQNRRPDYIKAFWSVVNWSFVEQQYEKAR"
     gene            10437..10793
                     /gene="ndhC"
                     /locus_tag="PLW25_02775"
     CDS             10437..10793
                     /gene="ndhC"
                     /locus_tag="PLW25_02775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOS53443.1"
                     /translation="MPFEYIPIVILLALSIAVGIIVLLLGRYLGPYRPTKKKGEPYES
                     GMIPYGPGTRQMPVRFYLVAVLFILFDVEVIFFLPWAVTFQKLGLFAIIEMGIFVTIL
                     MTGFVYLWKKGAMEWE"
     gene            10784..11266
                     /locus_tag="PLW25_02780"
     CDS             10784..11266
                     /locus_tag="PLW25_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOS53444.1"
                     /translation="MGVEPKSGSSGVVTTSLDKMVNWARTGSLWPMTFGLACCAIEMM
                     AMQASNYDMSRFGMELMRASPRQSDLMIVAGRVSQKMGPVLRNLYDQMPDPKWVIAMG
                     DCASCGGVYNNYAVYQGVDEIVPVDVYVAGCPPRPEGLIHGILTLYEKIRTEKVSDWK
                     "
     gene            11274..11786
                     /locus_tag="PLW25_02785"
     CDS             11274..11786
                     /locus_tag="PLW25_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOS53445.1"
                     /translation="MNEILSPAVERIQQQFKAQTSEFRGEVTLLISADANVEAATVLR
                     DEFAFDMLSVVTAVDYCPQTNPRFHLVYIFYSTANHAYLNVRVPLDGDAPTIRTLETV
                     YPSANWREREVWDLFGIRFEGHSDLRRLLMPPDWEGHPLRKDYPLGYEEVQFTFNFDE
                     IDIRKPKGVL"
     gene            11789..13018
                     /gene="nuoD"
                     /locus_tag="PLW25_02790"
     CDS             11789..13018
                     /gene="nuoD"
                     /locus_tag="PLW25_02790"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HOS53446.1"
                     /translation="MREIQEFTLEELKHHLSEKAFSGQTVVLNMGPQHPSTHGVLRLI
                     LELDGEIIVNCIPDVGYLHTGIEKTMESKTYQKAEVLVDRMDYMYAMGNTLVYSLAVE
                     KLIGLQPPPRAEAIRVILTELSRIQSHLLWLGTSALDLAAQSTFLYTMRERELILDIF
                     ELVSGARMMTTFIRPGGLWRDVPVQFEKMVRDFTKVMPKRMAEYESMLTRNPLFLDRT
                     KGIGKIDTRTCLAYDVTGPTLRASGLAHDVRKSNPYSGYDLYDFDIPTQSEGDVYARY
                     LVRMEEMRQSVRIIEQAMDKLPYGPVRDPNYKYVPPPRSEIGVSMEALIHHFKLWTEG
                     FIPPKGAVYTAVESPRGEMGVFMESNGTPKPYRVHYRTPSGPLLHALPVIAKGHFVAD
                     MIGIIGSIDIVLGDCDR"
     gene            13015..13569
                     /locus_tag="PLW25_02795"
     CDS             13015..13569
                     /locus_tag="PLW25_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOS53447.1"
                     /translation="MNQLLKKFPQEIKKILAKYPPDQKRASVMPLLHLAQQDRGYISR
                     ETMAEIAEIAEVEVTEVASVVGFYTLFHTEPGGKIRIQVCTDIACALRGSREYLDGLC
                     AELDIKPGGTTPDGLVTVEEVKCLAACDRAPMFQSQMGDELEYHEHQTVARTMDWLRS
                     MMASLRLPESDEPLSPEPEGGDHE"
     gene            13562..14812
                     /gene="nuoF"
                     /locus_tag="PLW25_02800"
     CDS             13562..14812
                     /gene="nuoF"
                     /locus_tag="PLW25_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOS53448.1"
                     /translation="MSELWLLRHRQIPGLNQLSTYLQHGGFESWKKAVTKLTPEDLVN
                     EVKNSGLRGRGGAGFPTGAKWSFLPNTIWPHYVVANADESEPGTFKDREILEFNPYQF
                     LEGLMLASYAVRAHTAYVYLRGEFWHIAHKLDERIAELTSSGYLGDNLFGKDYSLRIH
                     THLGAGAYICGEETALLESMEGRLGQPRLRPPFPAVSGLFGYPTVINNVETLTNLPLI
                     IGKGAAYFRAQGTEKSPGTKIFSLSGRVKKPGNYELPLGTTFRELIYQHGEGIINDAQ
                     IKSIMPAGASSSLIPATEVALDTPMDYESVGALGCALGSASVIILDETVNASTVVSDT
                     IHFFKHESCGKCTPCREGTFWMARVIDRIEAGKAKEEDIDLLASIASQMQNKCLCALG
                     EFSTVSVISAIKNYRADFAARLEK"
     gene            14817..>16881
                     /gene="nuoG"
                     /locus_tag="PLW25_02805"
     CDS             14817..>16881
                     /gene="nuoG"
                     /locus_tag="PLW25_02805"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HOS53449.1"
                     /translation="MEKQVTLKIDGRSVTVPEGTLIVDAAKQLGIVIPVFCYHPKLKP
                     AGMCRVCLVDVGRPQLDRASGAPVLNEDGTPKIVFGPKLETACTTPVSEGMVVWGATE
                     KVMEARKEVLEFLLTSHPLDCPVCDKGGECPLQNLTMKFGPAESRFEFDEKSHNQKRV
                     PLGELIILDRERCIHCGRCVRFQEQIVDDPVIGFYNRGRALEIRTYSDPGFDSIFSGN
                     TTDICPVGALTTADFRFGARPWEMKDKASICTQCPVGCNITYNVRREARKDGRTVIKR
                     VMPRQNEAVNEIWICDKGRFTYHYYESPERLTAPLIRRGDELVQTSWDEAIRYAAQQV
                     KAAGSRLAMVTSGRLSNEDLFALKQLADVTGGKAILHGTISGGEAVSLAGMSPGSDLG
                     TLAQGSTILVFACDLHEEAPIWWLRVKQAAERGATLIVVNPRTTRLDKFATHRLSYAY
                     GNELDMLQTLQADTGDIGQAVKEAENLVFIFGSEGMGLSASAAVAEQLALWLERSGHR
                     GKPSSGLIAVWQKGNEQGAWEIGYTTDERLAETISAAMGVYICAADPASEDPELKIAL
                     QRAGFMIVQDLFLTETAKLADVVLPAQAVMEREGTAVSGERRLQYYSPAVPAPAGTRP
                     DYAIIRAIAEEAGIQLKGENTADLFEQLTDSVEVFKGITLKEVRAVREQKPALGRSHM
                     YYGGTS"
     assembly_gap    16882..16981
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16982..17449
                     /locus_tag="PLW25_02810"
     CDS             <16982..17449
                     /locus_tag="PLW25_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53450.1"
                     /translation="REQKPALGRSHMYYGGTSYENREGLGVTLPLRAGALRSSAEHYQ
                     PQALTLESGSLLAVPITRLYDRGGTVATSGMLQQRIVRSLFSLHPADAELFQVKAGER
                     ISLRLDSREIEGEVDINIGQPQGVVLVTRSMGALLSAPVVVKLTVPQRSAVNS"
     gene            17462..18466
                     /gene="nuoH"
                     /locus_tag="PLW25_02815"
     CDS             17462..18466
                     /gene="nuoH"
                     /locus_tag="PLW25_02815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOS53451.1"
                     /translation="MTFAMFLEWFLKALILVLGLTVGFAYVTLFERRLLARMQVRIGP
                     NRVGPAGLLQPVADAIKLIFKEELIPAKADKILFVLAPVLTVIPALIITGVIPLGPPI
                     RLFGREIALQVANLNIGILYLLTVTAISIYGIALAGWSSNNKYSTMGALRATASMISY
                     ELALGLAYVGPVLLAGSMNINDIVTAQKPLWFVVMQPLGAIIYLIGANAEINRTPFDM
                     PEAEHELTAGFLTEYSGMKFALFYMAEYIKMIAVSMIGASLFLGGYWGPGVSTYPWLG
                     PIYLAIKVILLLFWFIWVRATWPRFRYDRMMAFGWKILFPLALLNVLVTALVIVLIGG
                     "
     gene            18470..18961
                     /gene="nuoI"
                     /locus_tag="PLW25_02820"
     CDS             18470..18961
                     /gene="nuoI"
                     /locus_tag="PLW25_02820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOS53452.1"
                     /translation="MLRGLITTFKSLFEKPVTIQYPEQRRPVFERYRGRHELKRYDNG
                     LEKCIGCALCAAACPSDAIFVEAAENTDDERYSPGERYASTYEINMLRCIYCGFCEDA
                     CPTEAIVLEKIYEMSYSDRHSAIFTKDMLLVKVPDGGQPTPQKTEPGLYTRSVPEMKD
                     PTD"
     gene            18972..19490
                     /locus_tag="PLW25_02825"
     CDS             18972..19490
                     /locus_tag="PLW25_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOS53453.1"
                     /translation="MTPALLIFILLGIIAVASALAMLLSRNTVYSALFLILNFLAVAV
                     IYILMGASFVALSQIAVYAGAIMVLVLFVIMLLGTQQLDPRETLKWQRPLAIVLTLAL
                     IIQGAYALITRYGFQYATLAASPEFVDPKNLGMVLFQKHSLPFEVTSVILLVAAIGAV
                     VLTHKQKKDGGA"
     gene            19490..19792
                     /gene="nuoK"
                     /locus_tag="PLW25_02830"
     CDS             19490..19792
                     /gene="nuoK"
                     /locus_tag="PLW25_02830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOS53454.1"
                     /translation="MFPVIYPIILAVILFSLGVAGVLLRKNAIVILMSLELMFNAANL
                     VFITFANYHQNLDGQVFIIFIMVTAAAEVAVGLALIVNIFRAKKTISIDEMHSLKG"
     gene            19819..21774
                     /gene="nuoL"
                     /locus_tag="PLW25_02835"
     CDS             19819..21774
                     /gene="nuoL"
                     /locus_tag="PLW25_02835"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOS53455.1"
                     /translation="MESGQIFNLVPWVVFLPVIGLVVNLLLGRRLGEVFSGVVASLAS
                     GLTFVVAVLLAAALASAPNARVVSPWEWISIGELTVRWAFRVDTLSVVMMLAVSLVGT
                     LIHIYSIGYMHSDVRHNGDPGRYTRFFVFMNLFIASMMLLVSADNFLMMFVGWEGVGL
                     CSYLLIGFWYEKGKNGIGNAEAAKKAFITNRIGDFGLLIALFLIFGYFGTLQFDEVFR
                     QAASVAAAHPGAMLLITLAMLLGVSGKSAQIPLFVWLPDAMAGPTPVSALIHAATMVT
                     AGVYLVARSTAFYSVVPVAQTVVMWVGALTALLAATIALGQYDIKKVLAYSTISQLGF
                     MVAAVGMGAMVAGMFHLVTHAFFKGLLFLSAGSVIQGMERGMHGGDDSAEFDPQDMRN
                     MGGLRKKMPLTFWVYLIGGLALSGIAPLAGFFSKDEILGAAYTGHNAVFVLLVLTAFL
                     TAFYMGRQLVMVFFGKPLSAAAEHASESPRVMTIPLVILALLSLLGGALNLPGVHTLE
                     HWLAHTLGEVKPAEFIWLVAGLSFLLALLGLGLAWLIYTRREQKSGADDPLKKPLGAL
                     YTGMERKWWVDEFYQALILNPYKAFAQFIAEPVDLGIIDRVGGGLAAGTRALAEGLRQ
                     LENGYVRSYALWMVVGLLAIMMFLFLR"
     gene            21785..23290
                     /locus_tag="PLW25_02840"
     CDS             21785..23290
                     /locus_tag="PLW25_02840"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOS53456.1"
                     /translation="MNSAINPLLLVTFFPLVGVLLILLLGSTGKNTARWIALLTSLVT
                     FALSLWLLMQFDSTKGGIQMEINLPWLTLGGNTVGFHLGVDGISILMVLLTALLTPIA
                     ILSTWKAVEERVSGFMSFFLLLETGMMGVFLSLDMVLFYIFWEFTLVPMYFLIGLWGS
                     QRRVYAAVKFVLYTMAGSLLMLAGILWVGLTYGGFDLTKLTDSAIPFTAQLWLFGAFG
                     LAFAIKVPMWPLHSWLPDAHTEAPTAGSVILAGVLLKMGAYGFLRFNLPLFPQATLTL
                     APWMAGLAVIGILYGAIVAFRQKDVKRLVAYSSISHLGFVVLGTFALTAQGVQGAILQ
                     MVNHGLSTGALFLIVGMLYERRHTRLMADFGGLWRVTPLLGSLTLVTVLSSVGLPGLN
                     GFVGEFTILMGAFHSTILASPWFAVVATLGVILAAVYLLHMFQQVFLGPVRHPENEKL
                     ADLSPREVILLAPLLLLAFWIGLYPAPFFRLMSPAVEQLLALIQTVVQTAH"
     gene            23305..24750
                     /locus_tag="PLW25_02845"
     CDS             23305..24750
                     /locus_tag="PLW25_02845"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOS53457.1"
                     /translation="MTLIDFNSILPVILLVVWALVMLLLDVWFGKRVPLLTPVMAVAG
                     LVLSLAASLYQIGQQASAFNGMIRVDGFSIFLNNLFALSGIVAIGLGYDYLKRVGISE
                     GEYYVLLLFSISGMMLMGSAGDLIIVFLSLELLSIPLYVLAGIDHKRDASREASLKYF
                     LLGTMASAFVLFGIAFVYGAAGTTSLYGITEAVSQGKVQQFLLICGSALLLAGFGFKV
                     AAVPFHSWTPDVYHGSPSSVTAFMSVGAKAAGFAALIRVFSLNFTAIAIELTPLFWVL
                     SALTMIVGNVVAVAQTNLKRMLAYSSIAHAGYILMAFVGFGDAAVRQNSIASALFYLA
                     AYALTSLGAWSVLVAVEQQDGSGNEISDLAGLGKTAPSLAAAMVVFMLSFTGIPLTLG
                     FWGKFYLFRTAIEGGFTYLAVAGLLTSLVSAFYYLRVVVKMYFQPGEALAHKGRLTSL
                     IATILAILVVGLAFVPGYLFNLAAAGLIAGA"
     gene            complement(24817..25470)
                     /locus_tag="PLW25_02850"
     CDS             complement(24817..25470)
                     /locus_tag="PLW25_02850"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HOS53458.1"
                     /translation="MWYYTLNNQQVGPVEEAEIKKLVEAGTITHGTLVWTNGMANWLP
                     IGQTPLAGLLGSVPPPVAPPPMAVVYEDPEVASMKKLFMWFWISLIGILLSGIGLIPA
                     GVLFFVIFYKAWKQIEHEGIRANADQATSRCFIPGWNYYWLFVAFRGLAKEQGDKLDS
                     LNVSLEKIDPKLPTWMLICLLGSAVTFGVSLIAFLVLWIMYTNKVKNNYLAIHQASK"
BASE COUNT         5607 a         6836 c         7363 g         5830 t
ORIGIN      
        1 ttgagcgcct gaaatatacc ccaacccacc agaaacgtca aggaaaacgc caactcatca
       61 ggctgatctc aaaagggctg gtcacctgcc ctttaaaagc acctaatgcc tgggtgaggg
      121 ggcacccaag catttgatag aagcagtata tcactcattt gcacaaatga cttaacaaat
      181 atgcaaactc cgcggtctcc ctgtttaccg ttacgattca ccaggggcca ggccgctgac
      241 cggttcaggc ttcggcaatt tccaccgtca gcagcttcac agccgggctg ttgcgctggg
      301 aggggtcgcg cgccgggatg gaaagcagca cgtccaatcc cttgatcacc tggccaaaga
      361 cagaatgctt attgtccagc cagggcgtgg gcacgtgggt aataaaaaat tgcgagccgt
      421 tggtgttcgg gccggcgttg gccatggaaa gtattcccgg ggcattatga cgaagcgagg
      481 aggcgaactc atctttgaac ttgtaaccag gtccgcccat accggttcct gtgggatccc
      541 ctccctgagc catgaagtca gggatgaccc ggtgaaagat ggtgttgtcg taaaaaccct
      601 cgcgcgccag gaagacaaag ttattgaccg tgcgcggggt ttttttggcg tgcaggctgc
      661 atatgatgct gcccaggtct gtcttgaagg tcgcggtgta ggatttctca gggtctatgc
      721 tcatttcggg aggagtagac cattgtttgg tttgatcgct catgctctac ctttctatag
      781 tggtgtggga aaaaattgta tcacatgttt tcagtagctc gcgcgcgtga gtagcgcctg
      841 gcgagagcgg taatgcccca aataattgca aaaaccgctg tacaggtcag cacgatggca
      901 gaaccggaag ccaggttgag gtaatacgaa aggtacaacc cgctgatacc ggagaaaatc
      961 gcgataagcg cggcaatcaa catcatgcgg gaaagccggc gcgtcagcag cgaagccgcg
     1021 gccgccgggg tgatgagcat cgccaccatc agggtaacgc ctaccgtctg cagagatacc
     1081 acgatgctca tggcgatcag ggcaatgagc aggtaatcga gccaacgaat aggcaggcgc
     1141 agcgtggctg ccagtgttgg gtcaaaggag atgaccagga attctttata aaaggtcagg
     1201 atgaagagca ttatcagcgc gccgaataca gcggtgagca gcaggtcagt gtcgcggacg
     1261 ccaagcacgt ctccaaaaag aaagtgcgtc aggtcagcgg tgtaattgcg taccattgaa
     1321 atgagcgcga ccccgagggc aaacatcccg gcgaagatga taccgattga agtatcctct
     1381 ttaatgcggg cggcgcggct gaccgcgccg ataccaaaag cgctgagcag ggcggtggcc
     1441 atggcccacc aaaataccgg ccctctcgcg ccgccgccca ggaggtaacc taccgccacc
     1501 ccggggagaa ctgcgtgcgc cagggcatcg ccaaagaagg ccattccacg cagcaccacg
     1561 taggtaccca acaccgcgca caccatgccc acaatcacgg ccgccagcat cccgcgaacc
     1621 ataaaggcgt actgcagcgg ggtccataac cagtctatca ttcttccctt ccgccgcagc
     1681 aggtgtcatc caccatggat agaccatgac cgttctccag cacctgagca cggtcaccat
     1741 acgccttgct gagatgctct ctggtaaata catcggcggg tttgccaata cccaccaggc
     1801 ggcggttgag gagcatgacg cggtcaaagc aggtggcagc cagtttaagg tcatgcagcg
     1861 cgaacatcac cgtcaccttc tcttctctta aagtatccag aacagtgaaa agttcttcct
     1921 gcgaaggcgt gtccagcccg gtcacgggct cgtccatcaa catcaggtcg gcttcctgcg
     1981 ccagggcgcg ggcgatgaac atgcgttgct gctgcccgcc cgaaagttgg ctgatctgac
     2041 ggtgggcatg agcgtgaagc ccaaccgtgc gcagggcgcg gctgacgatc tcgcgatcgc
     2101 cggcagaagg ccagcggaag aagccgattt tccgggtgcg ccccatcatt accacttcgc
     2161 ccacggttac cgggaaattc cagtccacgt ggttgtgctg cggaaggtag gaaatgcaga
     2221 tatgaccgct gggctcggct ccgaacacgc tcactgtgcc gcttccaggt ttcaacaagc
     2281 ccgcgatgat tttaaacaag gtgctcttac cggcgccgtt ggggccgatg accgccatac
     2341 gttcaccggt gatgagctga aaggtcacat tctgcagcgc caccacgcct tcataagcca
     2401 ggctgaggtg ctccacccgc aaaatgggtt caccgggaat atgcaccggg tgactgcgcg
     2461 gtgtgatggt gtttgcagtg ggttgggcaa ccgaattcat gtgtactatt ttccactcag
     2521 ggcgttgaca atggtcttca cgttgtagcg catgtaatcc aggtaagtag cagccggtcc
     2581 atcggcgttg gagagggaac cggtgtaaaa ctgcaccatt gccgtgcccg tatcttcggc
     2641 aacacgcgcg gccaatgtgg ggttgactgt gttacctacc agtatcaccc gcacgttgta
     2701 ctggcggatg gcgtcttcaa gcgcggcgat ttcctgcgcg gagggttggg ccagagagct
     2761 ataggaaggg atgagcgcac cgaccacagt gaaaccgtat ttttcagcga agtaattaaa
     2821 aagcatgtgg tccgtgacga tcaggcgttt ttcctgtgga atgcgcgcgg tctcacttct
     2881 aatccactcg tccaatgctt ttaattcagc gatgtaagcc gcggcattcg cctggtaggt
     2941 gccgctgccg gctgggtcac gcgcgctcaa cgcggcagcg atgttctccg tccaaacgat
     3001 cacgttggcg gggtccagcc acacatgcgg gtcggttgtg tgttcgtgct gctgttccgc
     3061 ggtgtttgca tcatgcggga ttaacaactc ctgggtggag atgccactgg atacctcaat
     3121 gggcgtaata tcgtcggacg cgttacggat aatggcgtcc ataaattgct ccaatcccag
     3181 tccaacttca aaaaccaagg cagcgtcaga taccgcagcg atgtcgcgcg gcgagggctg
     3241 gtattcatgc tcgtttacgc caggggggac aagggtggtg acggaaacaa ggttaccgcc
     3301 cacccggctg acaacatctg ccaggatgga agtggttgcc acaaccggca gctgtttggc
     3361 tgttggtgtg atctggccag tgcaggcact cgccagcagg atgataaacg agagcaagag
     3421 caggagcgat ttttttttat gcatggttga ccttcggtgt aacctgctgg ctattcaact
     3481 ggcagtcagg gcaggtacca aatagctgca gccagtgctc actcacctgg taaccggtgc
     3541 gtgattccac cttggtgaac aagggcgcca ggttgtctcc tttaaagaga atggtgcgcc
     3601 tgcaggtggt acaaacaaga taatgttcat gcccgtctgc agcgggcatt aaaaggtggc
     3661 agccgtcttc gcggtgcacg cgttgaatca accccagcga ttccaggcgt tccagtgtgc
     3721 gataaacggt caccagtcca atgccggggt gggtttgcct gcacagagcg tgcacctccg
     3781 ctggctgtaa agcacgggga ctgtcgatga gcacttcaag gataacccgc cgcggggagg
     3841 tgatgcggca gccatcttct ttcagggttt tcaaccagtg ttcgagcttg ttcaaggttc
     3901 ctcttattga aaacgttttt caataagagt ttaccacaaa acagaatccc gtcacggcgg
     3961 aattcttgaa aaaaatgatg ggttgcgttg aattaccttg ttttaaagaa aagattgccc
     4021 gatccgcggt ctttcacgcg gatgaggatg gtgtgggcgg cggaggaaaa attgctgctt
     4081 tgccaggtcc catggtcggc tgtgttgcct gcagcggcga tatagccggc tgggacgtgc
     4141 accgtgcctg aaccattgtc cataacctcg attttcaccg ctgaagtcaa cggcagaaaa
     4201 acggttactg ccccgctgcc gctttcgagc gtgaaggtca catcggtatc tgttggcaga
     4261 gcgagttcga ccgaacctga accactttca atatccacct ggtagggttg cggcgaatcc
     4321 ggcagggtga ttttggacgc cccagaaccg aaatccgctc gcagcgcctc aagggtgatg
     4381 ccttccagtt gcagatcgag cgcgccggag ccgccatcga gattaagggt ggtaggaacg
     4441 ctggggttca acccaatatc ccacttttgt ccgcccaacc ccagatcata cgagaaccag
     4501 gggccaggct cagtgcttgt gctcagggta acggtcttat ctgtatcacc tttaacgtca
     4561 aaatgcacct gaccgcggtg cacaatggtc gccttgacca ggtcggtgga atcgggcaag
     4621 gcgctgatgg tcactggcgg ataagaggta tccaagtaat attgcacgtg cgctgtgttc
     4681 tctagcggtt tcgaaaccac ttcagtctta atgcccgccg gcgggttatt ctccattaca
     4741 gggctgaaga agaggaaaac ggctaccgcc gcgactgtga gcaaaccgat cagtccgccc
     4801 gcccaggggg cccggtagcc aaaaagcagg tcaagcccta acactaccag cgccagcggc
     4861 cagagcctga gcaaaacact ccagttgaac gcaggtagaa aaccaaggtt gcgcagtaaa
     4921 agaataacac ccgcgccaat caggatcacc ggtaagaaca aagaaactcg cttttgattc
     4981 acagacatgg ctgcacctcg cagtgaaagg tttacttaaa ggatatacgc tttaatacct
     5041 cgagggttgc gcccgggttg tctgttacca gcgcgtcaca gttgagcagt ttgtaatacc
     5101 acaggcgcct gtcgggcgtg gggtccagcg ggcaaaccag ttgtccgcgc tggtgggcgc
     5161 ggcggacaaa gaaggggttg aggaagactc ctggccagaa cggaccgagc acctcgggca
     5221 gggggttggg ggtcaactgg gacatggtga tccaaccaat gggaatggaa gggtcaacag
     5281 ccttcaccgc ctgcaggcgc ggcagcgaga aggaaagcat caggctgcgc tcgagaatac
     5341 cgctggagcg cagttcagcg accaagcggc ggcacacctc ttcttcgagg aagcggtctg
     5401 tcttgagctc cagcgccagc gccacccccg gcggcaagat tcccgcggtt tctgccaggg
     5461 tgggaatacg ctcgttttca aagcctttga agttgtaaga cgcgctcaac tgcttgagct
     5521 catccagggt gagagaattc accgcgcctt taccgttggt ggtacggtct accgtggcgt
     5581 catgaataca catgaacacg ccgtcggcgc tgaggtgcag gtcagtctcg aggatgtcag
     5641 cgccttcttc gaatgccttc agaaaggcag atagggtgtt ctccgggcac agcacgcggt
     5701 tgccgcggtg agccattaca taaggcttcg ccctaccggg caggcttaat tcacgcattt
     5761 cgctcctcct tggttgggtt aagggtgatg tcaaatctaa agcggcagtc ttcttcgaac
     5821 cacataggaa agttggtgcc ccacaggttg ttgaacaggc agaagtgcgc gccctggcgg
     5881 gtatccggct ggtcattatt gaaatccagc aggctgcggg cgcccaacgc caccagcgga
     5941 gaatcgaggg cggagatgcg caattcgctg cctagggcgc ggcatgtcac atagcgcccg
     6001 ctaccatgca ggtggcggtt gccgttttcc accacgctca gcgctggaat ctcccgcccc
     6061 agtttctcga tcgaccactc ggcgtcaggg tgatgctccg gtctaaaagt catccacagg
     6121 gcttcgggca tacgcgaggc ttgtttgttg aaccattgca ggtcgatgtg caaacgctgc
     6181 tcctgcgacg gtagagagta ctgcagataa ccattgcgcg gcgcgccgaa ttggtgcaaa
     6241 gcctcgtctt caaaggtgag ataaaagaga atgtgttcaa ccccaggcgt ggagcgcttg
     6301 aatattcctt ttaccagcgg caaccagcag cggctggggc tgtcttcggg gcgcaggccg
     6361 ggtttacaga aatcttccag cgcccagccg tggcgctgct gcggcaatac ataggtgttg
     6421 aagaaacgct cataatcctc gctggaaaag gtctgatatg agaacacact gaactcacct
     6481 gggcgcacaa ccaggctttg cccggttgtg cgatccacca gctggtcgag gctgcctttc
     6541 tgttgttcaa atgtcacagc gagggatggg ttttccagct ttgcctgggc gggttcgatt
     6601 ggctgccagg cgctcaggtc tggcactcgc gggcgaatgg attccaggcg ggcgcgggct
     6661 tcatctgcca gcgagccacc tcctaacgcg ttgactgcca gttgcgggta agcgcgcttc
     6721 tcaacccagg aggcttcgaa gcgctgaaag ttcggctgcc cgcgcgcgag gttgaaatct
     6781 ttcacagtgt agttttcatg gtcattcagc caggtctttt catccatccc ccaggtgtgt
     6841 tcgggtacca tgagcagcca gcggtttaag ttaatgaggg atggttcact ccgcgttact
     6901 cgaccgctgg cgagccattc agcccgtaag cgcagcaact cgcgaaaacg cgcaaccttg
     6961 atggggtcgc tgccaacacc gtggatccag gtatcaccaa tctcctgcga tagcacgggc
     7021 aggttttcgc gcactcgcac caactgcgcg gcaaaatcac tgagtgtgga agcgaacaca
     7081 cgcgctcctg gctggcggga gcgcacctct cgatacacat ccagcacctg ctcgtgagat
     7141 tgcgggccca tgttatccat ggtatgacca aacgccaggg agtgctccaa cccgggtatt
     7201 tcgaaaacgg agccgtaacc gctctcgtac attaccagca actccgcgcc gccagggtct
     7261 tgccagcgga acaggcttgg cacggagggc acggtggagc ccggattcac accgatgtgt
     7321 aaaaatacca cacctgcttc tgccagcagc ggaatgatcg cccgtgtatg acccggcaca
     7381 tcggtccatt tggcggcgat ggtatggcgc ccaaagcgtt gatccagcgt gcgcgaaagc
     7441 gacaggccaa agcggaaaag ctcggcgtcc atcagctcgg tgtgagtggt gaagggtagc
     7501 gcgtgccagg cgatatcccc cgccagaata gcctcttcca tctgcccgcg ttccgcggac
     7561 gaagcctgtt cgagatactc gtagataagc caggaaccgg tggtccagat gaacctgtct
     7621 gagcctgatt ggcgcagttc gcgcgccagg cgcagcgccg ccgggatata tttcttgaaa
     7681 taattccgca ccaccaccgc ggcgtaatcg gtaaaaccca catccaaatg agttttaaag
     7741 atcaggtgaa cgctgcgctc catcgattca gctcaacgaa gtctcggctt ggatgcgcgg
     7801 tagtaagagc acggaaagca ggaacaaccc gccgtagagg gcaaaaataa cgggataacc
     7861 cagccctggc tggatggcgt tgaagtagtc cgccagcgga ccgccgatac tggagccaat
     7921 tatgcccgcg cccgccccgg ccaggttgga aatacccagg tagcgcccag cgtctttctg
     7981 tggcaccagg tcagtaccca gcgcccagtt ggaagccatg aaagtgccgg tgcccaaacc
     8041 caggaaaacg ccggcaaaca tcaccatggg aatggtacgc gaaaagacca ggatgatggt
     8101 gcccagcacc gccagcgcgc cggcggcagc cagcaacttc ttgcgcccga ttttgtccgc
     8161 caggatgccg ccgacaatgg aggaaatgag caggaaaatg aaaatcaccg ccgtcaggta
     8221 ggtggtaatg gtggccgggt tgggcacctg cagcacatcc tttacaaaaa acatggcgaa
     8281 atttcgcact gagctgatgg ctgcaaggaa catcaggcgc gtgaccaccc accagatgaa
     8341 ggatttatgg gtgttggctt ctttgccgat gcccacccgc gcgccgaagt aaacgccgat
     8401 gaagatggag cctgccatac ccacaagccc cgccaaaccg accaggacga tacgcagcgc
     8461 caggctggta ctgtcctgcg gcagccaggt gccgatgcct ttaaccagca gaagagccag
     8521 ctgggtgatg cccacgaaga tgacggtcag cagcagcatc cgcaggacag gccggtcttc
     8581 ggcgcgcgga ggtttttctt ggagaggttc ttcgcgcgtg cctgctaccg tgatgagcat
     8641 ggtgaccagg aaggaaagca taaggatgag aatgaccagc cagaatttct gtcgatcaat
     8701 gagcggcgag atgagcaaca ccagcgcggc aggaaccatt tccatcactg atttaatgcc
     8761 cgaggcgcga ccgcgctgct gttctggaac aatgtcgggt atcaacccct gctgcccggc
     8821 ctggctgatg ttggaagaaa actgcagcag aataatgccc agtaatagca ctgccaggga
     8881 agccgtgatg ccgaaactgg attgtaagaa agcgttgaga ggtgagcttt taaaccacaa
     8941 agaagcgccc atcacgccta aaaaaagaat gttcaacaac gcacccacga tgatgaaagg
     9001 cctacgccgc cccatttttg aagtggaacg gtcgctgagc atacccatca cgggttgaat
     9061 gaacatggca accgccagac cggtgacgta taccagcgcg gtcgaggtat tcttcttttc
     9121 cggaggcata aagaccagca ccagcgctgg cagcagcagc gggctgatga cgttggaagc
     9181 gatactcaac cccaactgat agctattgag gaagagaagt tcgtaccatt tcagcaggcg
     9241 tttcatgggt accctccgca ggcaggattt cttcttaaat aacggatgag ttcagctaaa
     9301 ccagatcttc tttatagtat agttgcggag aaggcttgtc aagcattaga gagccgaaaa
     9361 gaacggttgg ttcgggaaac cacaggcgct aaataggttg tggtgaaagg gagcaactcc
     9421 ttcaacgtgt tttcttttta accgatcagc caagtgaatc ccatttctaa tattcttctt
     9481 ataaaaacct cttgactttt gttgtaaata tgattattat tatcttaata gtacgcaata
     9541 acaatgggaa gggaaatcat gaacaaggtt tttgaactac cgcaactcaa attcgccttc
     9601 gacgcgctgg aaccagccat tgacgcgcgc acgatggaga ttcaccacgg caaacaccat
     9661 gccgcctatg tcgccaacct taacaaggca ttagaagctt cgcctgaatt tttctccatg
     9721 ccaatcgaag acattttaaa aggcatcaat accgtccccg aaaccatccg caccgcggtg
     9781 cgtaataacg gcggaggtca tgccaatcac tccctgtttt gggacgtgct caacccgggt
     9841 ggaaaaagcg caccctccgg cgcgctgcta aaagccattg aaagcgcttt cggctctacc
     9901 gatgccttta tggaaaagtt cactgctgct gcgaccaccc gcttcggctc tggttgggcc
     9961 tggctggtgc tcgacggtca gaaacaattg caggtatact ctactgccaa tcaagatagc
    10021 ccatatatgg accatcacat tcccatcctc gggttggatg tgtgggagca cgcctactat
    10081 ttgaattacc agaaccgccg cccagattac atcaaagcct tctggtcagt ggttaactgg
    10141 agttttgtgg aacagcaata cgagaaagcc aggtaacacc ttgagacagc ctgaccaacg
    10201 gcctggcgca atagcgcttt atgattccgg taccgtctaa aaagggggaa gcggacagac
    10261 caaggcaagc gaataaagcc gcgccacaac cgtaaacagg gcaactcaat ttgctctacc
    10321 cccgccaaac ggcgggggtt tttttattga tatccatttt tcaggtaaaa tagagggaac
    10381 tgggaaagca gcatcatccc gggttgattg attctctcca acaggagatt gttcccatgc
    10441 cttttgaata tatacctatt gttatactgc ttgctctctc cattgcagtg ggtatcatcg
    10501 tactgctgct ggggaggtac ctgggaccgt accgcccgac gaaaaagaaa ggcgagcctt
    10561 acgaatctgg tatgatccct tatggccccg gtacgcgcca gatgccggtg cgattttacc
    10621 tggtggcagt gctgttcatc ctctttgatg tcgaagtgat cttctttctc ccctgggcgg
    10681 tcacttttca aaaacttggg ctgttcgcca tcattgaaat gggcatcttc gtgaccatcc
    10741 tgatgaccgg ctttgtttat ctgtggaaaa aaggagcgat ggaatgggag tagagcctaa
    10801 gagcggaagt tctggagtgg taaccacctc gctggataaa atggtcaatt gggcgcgcac
    10861 cggctcgcta tggcccatga cctttggctt ggcctgctgc gccattgaga tgatggccat
    10921 gcaggcttca aattacgata tgagccgctt cggtatggaa ctgatgcgcg ccagcccccg
    10981 gcaatcagac ctgatgatcg tggcggggcg cgtttcgcaa aaaatgggcc cggtgctgcg
    11041 caatctgtat gaccagatgc ccgatcccaa gtgggtcatc gccatgggtg actgcgcttc
    11101 ttgtggcgga gtatataaca actatgccgt ttaccagggg gtggatgaaa tcgtacccgt
    11161 ggatgtgtac gtggctggct gcccgccacg acctgaagga ctgatccacg gtattctcac
    11221 cctgtacgaa aaaattcgca ctgaaaaagt ctccgactgg aagtagaggc cgcatgaacg
    11281 agattctctc cccggcagtt gaaagaatcc agcagcagtt caaggcgcaa accagcgaat
    11341 tccgtggcga ggtgaccttg cttatttctg cagatgccaa tgtagaagcc gccacggttt
    11401 tgcgtgatga attcgccttt gacatgctca gtgtggtgac ggcagtggat tactgcccgc
    11461 aaaccaatcc acgctttcac ctggtgtata tcttttattc caccgccaac catgcctacc
    11521 tgaatgttcg agttccgttg gatggtgatg cccccaccat ccggacgctc gagacggtgt
    11581 accccagcgc caactggcgc gaacgcgagg tatgggacct gttcggtatc cgcttcgagg
    11641 ggcattcaga cctgcgccgg ttgttgatgc cgcctgattg ggaggggcat ccgctacgca
    11701 aagattaccc gctgggctac gaggaagtgc agttcacttt caactttgac gaaatcgaca
    11761 tccgcaagcc caagggggtg ctctaaacat gcgcgagatc caggaattca ccttagaaga
    11821 actcaagcac catctgagtg aaaaagcgtt cagcgggcag actgtggtgc tcaatatggg
    11881 acctcaacac cccagtacgc acggtgtgct gcgcctgatc ctcgagcttg acggcgagat
    11941 tatcgtcaat tgcattcccg atgtcggcta cctgcacact ggcattgaga agacgatgga
    12001 gagcaagacc taccagaaag cggaagtgct tgttgaccgc atggactaca tgtatgccat
    12061 gggcaacacg ctggtgtact cactggcggt ggaaaaactg atcgggctgc aaccgccgcc
    12121 gcgcgccgag gcgatccgtg tgattttgac ggagctttcg cgtatccagt cacacctgtt
    12181 atggctgggt acctccgcct tggacctggc agcccagtcc actttccttt acaccatgcg
    12241 cgaacgcgag ctgatattgg atatttttga actggtctcc ggtgcgcgta tgatgactac
    12301 tttcatccgt cccggcgggt tgtggcgtga tgtaccggtg cagtttgaaa aaatggtgcg
    12361 cgattttacc aaggtgatgc ccaaacgcat ggctgaatac gaatccatgc tcacgcgtaa
    12421 cccgcttttc cttgaccgca ccaagggcat tggcaaaatt gatacccgca cctgcctggc
    12481 ttacgatgta acaggaccca cgctgcgagc cagcggactg gcgcatgatg tgcgcaagtc
    12541 aaacccttac agcgggtacg acctgtatga ttttgacatt cccacgcaga gcgaaggcga
    12601 cgtgtacgcg cgctacctgg tgcgcatgga agaaatgcgc cagtcggtgc gcattatcga
    12661 gcaggcgatg gataaattgc cctacggccc ggtgcgtgat ccgaattaca aatacgtacc
    12721 acccccccgc tcagaaatcg gggtaagcat ggaggcgttg atccatcact tcaaactatg
    12781 gacggagggc tttatccccc cgaaaggcgc ggtttacacc gcggtagaaa gcccgcgcgg
    12841 tgaaatgggc gtgttcatgg aatcaaacgg cacccccaaa ccttatcgtg tacattaccg
    12901 cacgccctct ggtccgttgc tgcatgcctt gcctgtgatc gcgaaaggcc acttcgtggc
    12961 agatatgatc ggtatcattg gcagcatcga tatcgtactg ggagactgtg accggtgaac
    13021 caactgctta aaaaattccc gcaagaaatt aaaaagatcc tggcgaaata cccgccagat
    13081 cagaagcgcg ccagcgtgat gccgctgctg cacctggcgc agcaagacag gggctatatc
    13141 tcccgagaga ccatggctga aattgccgaa atcgccgaag tggaagtcac cgaggtggct
    13201 tcggtagtgg gtttttacac cctgtttcac actgagccag gcgggaaaat ccgcatccag
    13261 gtgtgcaccg acatcgcctg cgcgttacgc ggttcaagag aatatctgga tgggttgtgc
    13321 gccgaactgg atatcaagcc cggtggtaca acacccgatg ggctggtgac cgttgaagag
    13381 gtgaaatgcc tggcagcctg cgatcgcgcg cccatgtttc aatcgcaaat gggagatgaa
    13441 ctcgaatatc acgaacacca gacagtcgcc cgcacaatgg attggctgcg ttcgatgatg
    13501 gcttccttgc gcctgcctga gagcgatgaa ccgctatcac cagaaccaga aggaggcgac
    13561 catgagtgag ttatggctct tgcgccatcg ccagatacca ggtctgaacc agctctctac
    13621 ttacctacag catggcggtt ttgagtcctg gaaaaaagcg gtcacgaaat taacgcctga
    13681 agacctggtg aatgaagtca aaaattctgg tctgcgcgga cgcggcgggg cgggttttcc
    13741 aacaggcgcc aaatggtctt ttttacccaa cactatttgg ccgcattacg tggtggccaa
    13801 cgctgatgaa tctgaacccg gcacattcaa agaccgcgag atactggaat tcaaccccta
    13861 tcagtttctg gagggcctga tgttggcatc ttacgcggtg cgcgcccaca ccgcgtatgt
    13921 gtacctgcgc ggagagttct ggcatatcgc gcataagctg gatgagcgca tcgctgaact
    13981 gaccagcagc ggctacctgg gtgacaacct ctttggcaag gattactctc tgcgcatcca
    14041 cactcatctg ggcgcgggcg cctatatctg cggggaagaa accgcattat tagagtcaat
    14101 ggaagggcgc ctcgggcagc cgcggttgcg cccgcctttc ccggcggtct cggggctatt
    14161 cggctacccc acggtgatta acaacgtgga aacgcttacc aacttgccgt tgatcatcgg
    14221 caaaggggcg gcgtactttc gcgcacaggg aacagagaaa agccccggca caaaaatatt
    14281 cagcctatcc ggaagagtaa aaaagcctgg taattacgag ttgccgctgg gcaccacctt
    14341 cagagaattg atctaccaac acggcgaggg catcattaat gacgcgcaga ttaagtccat
    14401 catgccggca ggggcttctt cctcactcat ccccgccact gaggttgcgc tggatacccc
    14461 catggattac gagtcagtgg gcgctctggg ctgcgcgctc ggttccgctt cagtcatcat
    14521 ccttgatgaa acggtcaatg ccagcaccgt ggtgagcgat acgattcatt tcttcaagca
    14581 cgaatcctgc ggtaaatgca ctccttgccg cgaaggcacc ttctggatgg cgagggtgat
    14641 cgatcgaatc gaagccggca aggcgaaaga agaagatatt gacctgctcg ccagtattgc
    14701 cagccagatg caaaacaaat gcctgtgcgc gcttggagag ttctccactg tatccgtaat
    14761 ttcagcgatt aaaaactacc gggctgactt cgcggcccgc ctggagaaat aaacggatgg
    14821 aaaaacaagt cacactgaag atagacggcc gcagcgtcac tgtccctgaa gggaccctga
    14881 ttgtggacgc ggcaaaacaa ctgggaattg tcatcccggt gttctgctac catcccaagc
    14941 tcaagcccgc gggaatgtgc cgcgtttgcc tggtggatgt gggaagaccg caacttgatc
    15001 gcgctagcgg cgcgcccgtg ttgaatgaag acggcacacc caaaattgta ttcggcccca
    15061 agctggagac tgcctgcacc acgcctgtat cagaaggcat ggtggtgtgg ggcgcaactg
    15121 aaaaggtgat ggaagcccgt aaagaggtgc ttgagtttct ccttacctcg catccattgg
    15181 attgcccggt gtgcgataaa ggcggcgaat gcccgctgca gaacctgacc atgaagtttg
    15241 gccctgctga aagccgtttc gaatttgatg agaagagcca taaccagaaa agggtaccgt
    15301 tgggcgaact gatcatcctc gaccgcgaac gctgcatcca ttgcgggcgt tgtgtgcgct
    15361 tccaggagca gatcgttgac gaccccgtga tcggttttta taaccgcgga cgggcgctgg
    15421 agatcagaac ttattcagac cccgggtttg actcaatatt ctcaggcaac accaccgaca
    15481 tttgcccggt cggcgccctc accaccgctg atttccgctt tggcgcgcgt ccctgggaga
    15541 tgaaggacaa agcgtcaatc tgcacccagt gcccggttgg ctgcaacatc acctacaatg
    15601 tgcgccgaga ggcgcgcaaa gacgggcgca cagtgatcaa acgggtaatg ccgcgccaga
    15661 acgaagccgt gaacgaaata tggatttgcg ataaaggcag gttcacttac cattattatg
    15721 aaagccctga gcgcttgacc gccccgctca ttcggcgcgg tgacgaactg gtacaaacca
    15781 gctgggatga agccatccgg tacgcggcgc agcaggtaaa agccgctggt tccaggctgg
    15841 cgatggttac cagcggacgt ttaagcaacg aagacctgtt tgcgctcaaa cagctcgctg
    15901 atgtaaccgg aggaaaagcc attttgcacg gtaccatcag tgggggagaa gccgtctctt
    15961 tagcgggtat gtcccccggg tcagatctgg ggacactcgc gcagggttca accatcctgg
    16021 tgttcgcctg cgacctgcat gaagaagcgc ccatctggtg gttgcgtgtg aagcaggcgg
    16081 cagaacgcgg cgccacgctc attgtggtta accctcgcac tacccgactg gataaattcg
    16141 ccacccaccg gctgagctat gcttacggga atgagctgga catgctgcag acgctgcaag
    16201 ccgacaccgg tgatatcggg caggcggtga aagaagcgga gaacctggtc tttatttttg
    16261 gcagtgaagg catggggttg agcgcttcag cagcagtcgc tgagcagctc gctttatggc
    16321 tggagcgcag cggacaccgg gggaagccca gcagcgggtt gatcgccgtc tggcagaaag
    16381 ggaacgagca gggcgcctgg gagattggat acactacgga tgagcgcctt gccgagacga
    16441 tcagcgccgc gatgggggtg tatatctgcg cggctgaccc ggcgagcgaa gatccagaat
    16501 tgaaaatcgc cttgcaacgc gcgggcttta tgattgtcca ggacctgttc ctgaccgaaa
    16561 cagccaaact ggcggatgtg gtgctgccgg cgcaggcggt tatggaacgg gaaggcacgg
    16621 cagtgtcagg ggagcgccgc ctgcagtatt acagcccggc agttcccgcc ccggcgggca
    16681 cccggcctga ttacgccatc atccgcgcta tcgctgaaga ggcgggaatt caactgaaag
    16741 gtgaaaatac ggctgacttg tttgaacaac tgacggacag cgtagaggtc ttcaaaggta
    16801 ttaccctgaa agaggttcgg gcggtgcgcg agcaaaagcc cgcgctcggg cgcagtcata
    16861 tgtattacgg cggaaccagc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16981 ncgcgagcaa aagcccgcgc tcgggcgcag tcatatgtat tacggcggaa ccagctatga
    17041 gaacagggaa ggattggggg taaccctgcc gctcagggca ggcgcgcttc gttcatctgc
    17101 tgaacattac caaccccaag cgctgaccct tgaaagcggc agcctgctgg cggttcccat
    17161 cacccggctg tatgaccgcg gcggcacggt tgccaccagc ggtatgttgc aacaacgcat
    17221 tgtgcgcagt ttgttcagcc tgcatccggc tgacgctgaa ctcttccagg taaaggcggg
    17281 cgagcgaatt tcgctcaggc tggatagcag ggagatcgaa ggcgaagtgg atattaacat
    17341 cggtcagccg caaggggtgg tgctggtcac acgcagcatg ggggcgctgc tttctgctcc
    17401 ggtagtggtt aagcttaccg taccgcagcg aagcgcggtt aattcgtagg cgagggtagc
    17461 tatgacattt gcgatgtttc ttgaatggtt cttgaaagcg ctcatcctgg tcctcggctt
    17521 gacagtgggt ttcgcctatg tcacgctctt cgaacgtagg ctgttggcgc gcatgcaggt
    17581 tcgcattggg cccaaccgcg ttggaccggc gggtttgctg caacccgtcg cagacgccat
    17641 taagctgata tttaaggaag aattaattcc agccaaagcc gataagatcc tttttgtttt
    17701 ggcgccggtg ctcacggtca tcccggctct catcatcacc ggtgtcatcc cgctgggacc
    17761 acctatccgc ctttttggga gagagattgc actgcaggtt gccaatctca acattggtat
    17821 tctttatctg ctcaccgtga cggccatctc catttatggg atcgcgctcg cgggctggtc
    17881 atcgaacaat aaatactcca ccatgggcgc gttgcgcgcc actgcttcga tgatcagtta
    17941 cgaactggcg ctggggctgg cgtatgtagg gccggtgctg ttggcaggat cgatgaatat
    18001 aaatgatatt gtcactgccc aaaaaccgct gtggtttgta gtgatgcagc cgctgggcgc
    18061 gatcatttat ctcattggtg ccaatgctga aatcaaccgt accccgtttg atatgcctga
    18121 agcggagcac gaattgaccg ctggatttct caccgagtat tctggtatga agtttgccct
    18181 cttctacatg gcggaatata tcaagatgat agcggtttcc atgattgggg cttcgctgtt
    18241 tctgggtggc tattggggcc cgggcgtgag cacttacccc tggttaggac cgatttattt
    18301 ggctatcaag gtcatcctcc tgcttttctg gttcatctgg gtgagggcaa cctggccgcg
    18361 cttccgctat gaccgaatga tggcttttgg ctggaagatc ctctttccgc tggcgctgtt
    18421 gaacgtgtta gtgaccgcgc tggtgatcgt attgatcgga ggttgagaga tgctgcgcgg
    18481 attgatcact acttttaaat cactctttga aaaaccggtc accatacagt atcctgaaca
    18541 gcggcggcca gtgttcgaac gctaccgcgg caggcatgag ctgaagcggt atgacaatgg
    18601 gcttgaaaaa tgcattggct gcgctctatg cgcggcagcc tgcccgtcag atgccatctt
    18661 tgtggaggcc gctgaaaaca cggatgatga gcgctactca ccaggagagc gttatgccag
    18721 cacgtatgag attaacatgc tgcgctgcat ctactgcggg ttctgcgaag acgcctgccc
    18781 tacggaagcc attgtgcttg aaaagatcta cgaaatgtct tatagcgacc gccacagcgc
    18841 gattttcacc aaagacatgt tactggtgaa agtaccggat ggcggacagc ccacaccgca
    18901 gaaaactgaa cccggcttgt acacgcgttc ggtgcctgag atgaaagacc ccacagattg
    18961 aggaggaagg gatgacgcct gctttgctga tttttatctt gctcgggatc attgcggtgg
    19021 cttcagccct ggccatgctg ctcagccgca acacggttta ttccgcgtta ttcctgatcc
    19081 tgaattttct cgccgtcgcg gttatttaca tcctcatggg cgcgtccttc gtcgcccttt
    19141 cgcagattgc cgtatatgcc ggggcgatta tggtgctggt gctttttgtc atcatgctgc
    19201 tgggcactca acagcttgat ccgcgagaaa cgctcaaatg gcagcgcccg ctggcaattg
    19261 tgttgaccct cgccttgatc atccagggcg cttacgcgct gatcacacgc tatgggttcc
    19321 agtacgccac tcttgcggct agccctgaat ttgtagatcc aaagaacctg ggtatggtgt
    19381 tgttccaaaa acactcgctg ccttttgaag ttacctcggt catcctgctg gtagctgcca
    19441 tcggtgccgt ggtactgaca cataagcaaa agaaggatgg aggcgcctaa tgttcccggt
    19501 gatttatccc attatcctcg cggtcatcct cttcagcctg ggggttgctg gcgttttgct
    19561 gcgtaaaaac gcgattgtca tccttatgtc actggagttg atgttcaacg ccgccaacct
    19621 ggtgtttatc accttcgcaa actaccatca aaacctggat ggacaggtgt tcatcatttt
    19681 tatcatggtc acggccgccg ccgaggtggc tgtggggctg gcattaatcg tgaatatttt
    19741 ccgcgccaag aagaccatca gcatcgatga gatgcacagc ctgaaaggct gaccgacccg
    19801 caaaagggag aatattttat ggaatctgga caaatcttca atcttgttcc ctgggttgtc
    19861 ttcctgccgg taattggcct ggtagtcaat ttgctgctgg ggaggcgtct gggagaggtt
    19921 ttcagcgggg tcgttgccag cctggcttct gggcttacct ttgtggtggc tgtactgtta
    19981 gcagcggcac tggcttctgc gccaaacgcc agagtagtga gcccgtggga atggatttcc
    20041 attggagagt tgacggtacg ctgggctttt cgcgtggaca cactctcggt ggtaatgatg
    20101 ctggcggtat cgttggtcgg caccttgatc catatctatt ccatcggcta tatgcacagc
    20161 gacgtgcgcc acaatgggga tcctggcagg tatacacgct tctttgtctt catgaatctg
    20221 tttatcgctt cgatgatgct cctggtcagc gcggataact ttttaatgat gttcgtgggc
    20281 tgggagggag tggggttgtg ctcttacctg ctcattggtt tttggtacga gaaaggaaag
    20341 aacggcatcg gcaatgccga ggcagccaag aaggctttca tcaccaaccg catcggcgat
    20401 tttgggctgc ttatcgcgct gttcctgata ttcggttatt ttggcaccct gcaattcgat
    20461 gaggttttcc gccaggccgc cagcgtggca gctgcgcacc caggagcgat gctgttgatc
    20521 acgttggcca tgctgctggg ggtgagcgga aaatcagcgc agatacctct gttcgtgtgg
    20581 ctgcccgatg caatggcagg gcccacgccg gtttccgcgc tcatccacgc ggcaaccatg
    20641 gtcaccgccg gtgtgtatct ggtggcgcgc agcacagcct tttacagtgt ggttcccgtt
    20701 gcccagaccg tggtgatgtg ggtgggcgcg ctcaccgcgc tcctggccgc gaccatcgcc
    20761 ttggggcaat acgatatcaa gaaagtgctg gcttattcca ccatcagcca gctgggcttt
    20821 atggtggccg ccgtgggcat gggggcgatg gtcgccggga tgttccatct ggtgacccat
    20881 gcctttttta aagggctgct cttcctgtct gcgggttcgg tcatccaggg gatggaacgc
    20941 ggtatgcacg gtggcgacga cagcgctgag ttcgatccgc aggatatgcg caatatgggc
    21001 ggcttgcgca agaaaatgcc gcttaccttt tgggtctatc tgattggcgg tctggccctt
    21061 tcaggtatcg cgccgcttgc gggcttcttc tcgaaggacg aaatcctcgg cgcagcatac
    21121 accgggcaca acgccgtgtt tgtgctgctg gtcctcacgg ctttccttac cgcgttttat
    21181 atggggcggc agctggtgat ggtcttcttc ggaaagccgc tctcagcagc agccgagcat
    21241 gccagcgaaa gcccgcgcgt aatgaccatc ccactggtca ttctggcgct gctttcattg
    21301 ctgggcgggg ctctcaacct gccgggcgtg cacacgctgg aacactggct cgctcacacg
    21361 cttggcgagg taaagcccgc agagttcatc tggctggtgg cggggctctc gttcttgttg
    21421 gcgttgctag ggctgggttt ggcctggctc atctacacgc gcagagagca gaagtccggg
    21481 gcggatgacc cgctgaaaaa gcccctcggc gcgttataca ccggtatgga acgcaagtgg
    21541 tgggtagacg aattttacca ggcgctgatc ctcaatcctt acaaagcctt cgcccaattc
    21601 atcgctgaac cggtggatct gggcatcatt gaccgtgtgg gcggtgggct ggcagctggt
    21661 acgcgcgcgc tggccgaagg gttacgccaa ctggaaaatg gctatgtgcg ctcttatgct
    21721 ttgtggatgg tggttggtct gctggcaatc atgatgtttc tattcttaag gtaaaggacg
    21781 ggcgatgaat tcagccatta atccccttct gctggtcacc ttctttccgc ttgtgggcgt
    21841 cttgctgata ttgttattgg gaagcacagg caaaaacact gcgcgctgga tcgccctttt
    21901 gacctcgctg gtcacctttg cgctttcact gtggctgctg atgcagtttg acagcacgaa
    21961 aggcggcatc cagatggaga tcaacctgcc gtggttgacc ctgggcggca acacggttgg
    22021 ctttcacctg ggtgtggatg gtatcagcat tctcatggtc ttgctgaccg ctttactgac
    22081 gccaattgcc atcctttcca cctggaaggc ggtggaggaa cgcgtaagtg gttttatgag
    22141 cttcttcttg ctgcttgaaa cgggcatgat gggcgtgttc ctctccctgg atatggtatt
    22201 gttttatatc ttctgggagt ttacgctggt gcccatgtac tttcttattg ggctgtgggg
    22261 cagccagcgc cgggtttacg cggcggtcaa gttcgtgctc tacaccatgg ccggttcatt
    22321 gctgatgctg gcgggtatcc tgtgggtggg gttaacctac ggcggctttg acctgacaaa
    22381 actgacggac tcggcgattc cattcaccgc acaactatgg ttgtttgggg cgttcgggct
    22441 ggcgtttgcc atcaaagtgc ccatgtggcc gctgcattcc tggctgccag acgcccacac
    22501 tgaagcccct accgccgggt ctgtcatcct ggcgggtgtg ctgctcaaga tgggtgctta
    22561 tgggttcctg cgtttcaacc tgccgttatt cccacaggcg acgctcacac tggctccgtg
    22621 gatggctggg ctggcggtaa tcggcatcct ctatggcgcg attgtggctt tcaggcagaa
    22681 ggacgtaaaa cggttggttg cctattcttc catcagccac cttggttttg ttgtactggg
    22741 cacatttgcc ctcacagcgc agggcgtgca gggagccatt ttgcagatgg tcaatcatgg
    22801 gctttccacc ggcgcgctgt tcctcattgt agggatgctg tatgaaaggc ggcacacacg
    22861 cctgatggct gattttggcg ggttgtggcg cgtcactccg ctgttgggca gtctcacact
    22921 ggttaccgtc ctctcctcgg tagggttgcc tggactgaat ggttttgtgg gagaatttac
    22981 cattctgatg ggcgcttttc attcaactat cctggcttcg ccatggtttg ccgtggtagc
    23041 cacgcttggt gttatcctgg cagcggtgta cctgctgcat atgttccagc aggtattttt
    23101 gggaccggta cgccatccgg aaaacgaaaa attggccgat ctcagcccgc gcgaggtcat
    23161 ccttctggcg ccgctcctgt tgttggcttt ctggataggg ttgtatccgg ccccgttctt
    23221 caggctcatg tcgccagcgg ttgagcagtt gctggcgctg attcagactg ttgttcagac
    23281 tgcgcattaa cggaaggtga aaatatgact ttaattgatt tcaactccat acttccggtc
    23341 atcctgctgg tggtctgggc attggtcatg ctgctgctgg atgtgtggtt tggaaaacgc
    23401 gttcccctgc ttacacctgt tatggctgta gctggactgg tgctgtcttt agcggcgagc
    23461 ctgtaccaaa ttggacaaca ggcttcagcc tttaacggca tgatccgtgt tgacggtttc
    23521 agcattttcc tcaataactt gttcgccctc agcggcattg tggcgattgg attggggtac
    23581 gattacctca agcgtgttgg catcagcgaa ggtgaatatt acgtcctgct gcttttcagt
    23641 atcagcggca tgatgctgat gggcagcgca ggcgacctga ttatcgtttt cctctcgctg
    23701 gaattgcttt ccattcctct ttatgtgttg gcaggtattg accataaacg cgacgcttca
    23761 cgggaagcat cgctgaagta ctttttatta ggaacaatgg cttccgcttt tgtgctcttt
    23821 gggattgctt ttgtttacgg cgcggctgga acgaccagcc tgtacggcat caccgaagcc
    23881 gtcagccagg gtaaggtgca gcagttcttg ttgatttgcg gtagcgcgct gttactggct
    23941 ggtttcgggt tcaaggtggc cgcggtgcct tttcatagct ggacgcccga tgtgtaccac
    24001 ggttcgcctt cttccgttac ggcttttatg tcagttggcg ctaaggctgc agggttcgct
    24061 gcactcatac gcgtcttttc gctcaatttc accgccattg ccattgagtt aacgccgctg
    24121 ttctgggtgt tatcggcatt gacgatgatc gttggcaacg tggtcgcggt ggcgcagacc
    24181 aatctcaaac gcatgctggc ttattccagc attgcgcacg ccggttacat cctgatggct
    24241 tttgtaggct ttggagatgc ggcagtacgc cagaattcta tcgcttccgc gctgttttac
    24301 ctggctgcgt acgcgctcac ttcacttggc gcctggtcgg tgctggtggc ggtagagcaa
    24361 caagatggca gcgggaatga gatcagtgac ctggcggggt tggggaagac cgcgccttcc
    24421 ctggctgcag cgatggttgt gttcatgctc tcgttcactg gaatcccgct caccttgggg
    24481 ttctggggca aattctacct gttccgcacc gccattgaag gcggctttac ctatttggcg
    24541 gtcgccgggt tgctcacttc gctggtctcg gcgttctatt acctgcgcgt ggtggtgaaa
    24601 atgtacttcc agcctggtga agccctggcg cacaaagggc gcctgaccag tttgatcgct
    24661 acgattctcg ccatcctggt tgttggcctg gctttcgtcc ctggttacct ttttaacctc
    24721 gctgccgccg gtctaatcgc aggggcgtaa atacttctac atcacaaaaa agtcccgcca
    24781 tgtggaggcg ggactttttt tatctattga attaagttac ttgcttgcct ggtgaatggc
    24841 gaggtaattg ttcttgactt tattggtgta cataatccaa aggacgagga aggcgatcag
    24901 agatacacca aacgtgaccg cgctccccaa caggcagatc agcatccagg tgggtagttt
    24961 cgggtcgatc ttttccagag aaacgttcag gctgtcaagc ttatcgccct gttctttggc
    25021 gaggccgcgg aaggcgacaa acaaccagta ataattccag ccggggataa agcaccgcga
    25081 ggttgcctgg tcggcgttag cgcggatacc ctcatgctcg atttgtttcc aggctttgta
    25141 gaaaatgacg aagaacagta ccccggcagg gatgagtcca atcccagaca gcagtatgcc
    25201 aatcaggctg atccagaacc acatgaagag ctttttcatc gaggcaactt cagggtcttc
    25261 atacacaacc gccattggtg gcggagccac aggaggtggt acagaaccta aaagccctgc
    25321 gaggggggtc tgtccgattg gtagccagtt ggccatgccg ttggtccaca cgagggtgcc
    25381 gtgtgtgatt gtgcccgctt caaccagttt cttgatctcc gcttcttcca cggggccgac
    25441 ctgttgattg ttgagcgtgt agtaccacat atctcttctc cttgtaaagt gtatttatac
    25501 gtatgatcga ctacatcatt ataatttatt tttgagcaac tcaaccttaa aacaacctga
    25561 caaagccgtt ttttgggcgt tattcttctc tttccggcct gaaatgacgc aggaatttaa
    25621 ttaacgcccg ccttttcttg gcttcaaggc gtttcgtgat gtcgctggca cgcggcctgg
    25681 taggaatgcg gttttgcggg gtttgttgcg cctgttcgac caggcgaaaa agacgg
//
LOCUS       DAOSPM010000017        24989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_1999_length_24989_cov_15.422355, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000017 DAOSPM010000000
VERSION     DAOSPM010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24989
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_24989_cov_15.422355"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLW25_02855"
     CDS             complement(<1..753)
                     /locus_tag="PLW25_02855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS53459.1"
                     /translation="MTLQRHTPVEASQAGKDHLWLQISSLPYFRALLRAVEARFYENI
                     DLPAPALDLGCGDGHFASLAFNRALEVGLDPWEKPLREAEARGVYQQAVLTKASEIPY
                     PDAYFASAVSNSVLEHIPALEPVLAELGRVLRPGAAFVFCVPNQRFLGTLSIGRFFDN
                     LRLRFLGDLYRRFFNRISRHHHCDDPSTWRQRLEGAGFELVDCWDYFSPRALRVLEWG
                     HYFGLPSLLTRLLWGRWIVAPWKWSLFFTRLIT"
     gene            complement(911..1741)
                     /locus_tag="PLW25_02860"
     CDS             complement(911..1741)
                     /locus_tag="PLW25_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOS53460.1"
                     /translation="MTNQSIDLAQLFGSVVSTLDQHKAALNQADTYNHNHGDNMVEIF
                     EVITQAMKAKSNATPAEQLAYASQLLQTKSQSSSAKLYAQGLQDASAQLGGKKTLTTN
                     NALTLLQALMGTQKKVTPQTLQTQARKTFEQQPSGQSAGGDLLGSLLGGLAGAQQTPS
                     GAGAGGDLLGSLLGGLAGAQQAPAGAGTGDDLLSSLLGGLTGAAAPSGSKPVKIDTAQ
                     LLKAGMAYMQAKQSGSSDINALLSALMAGSRMGTQNYRTQSGTLVANSLLKALAQMSQ
                     "
     gene            1853..2422
                     /locus_tag="PLW25_02865"
     CDS             1853..2422
                     /locus_tag="PLW25_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOS53461.1"
                     /translation="MNDKKLPLNAAEYALLGFLVAGPCHGYELHRKLTEPDGAGMIWG
                     IKISNMYAQLDKLERRGLISGRLLENEQRPARTQYSLTAAGKQLFEEWLHQPVQHPRD
                     FRHFFFLQLFFLSQYEPDALESLVERQLELCQAWQQNLLAAQERSSTSVDFADLTNRF
                     RHSQIQSMIDWLFWIKSEKIKIKPKRGNQ"
     gene            2419..3582
                     /locus_tag="PLW25_02870"
     CDS             2419..3582
                     /locus_tag="PLW25_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS53462.1"
                     /translation="MKRPLQFLVVLVLFSSLLAACAAPTPAAAPSLTEPPATEAPAAV
                     STPAPTEPPEMEKITVVDALGREVVFDQPPSRIMIVGKAAFMLLDAAFLFPEAPEKII
                     GYELRSQTGLDFIHTAFPQSHNMIVLEMDAGPEQIAPANPDLVLMKSYLKEQLGDQVE
                     LLGIKVVYLDLETPETINQDIRTLGKLFGNTERAEQLVTLYDQARAKIADITNVIPEE
                     EKPSTLLLQYSDKGGEVAFSVPTGGFLQTQLVEMAGGAPVWKEVTGKGWSVITLEQIA
                     AWNPEIIIVVDYRGKAMEAVDRLKADENWQQLDAVKNEKLYAFPLDFQSWDQPDSRWT
                     LGLTWLATIMQPERFASVKMHEEIPAFYTDFYGLSESVIEQSILPLVKGSFLE"
     gene            3621..4613
                     /locus_tag="PLW25_02875"
     CDS             3621..4613
                     /locus_tag="PLW25_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOS53463.1"
                     /translation="MKNNKNLTAFVLVVTPLIVLGISLFIGRYPKPLLMPLSVLLHDQ
                     LGQNLVYNLRLPRLITSALLGMSLATAGGVMQMLFRNPLVEPGFLGVTQGAGFGAALS
                     ILFLTTAPLVVELSAAGFAIIGLLLSYFIARRIRFGGWVLRLVLSGIAVSAIFSAGLG
                     ILKYMADPTSQLPEIVFWLLGGLWSVTWTDLWYILPVVVIGLVIIYLMRWRLNLLSLS
                     DETAFSLGVAVGRERTLLLAASVAITAVVVSVAGIVGWIGLIIPHIARRIAGANAASF
                     IPVSMSLGAVFGIICDDLARVLTTGEVPLGIITSLIGAVIFLIMMTSRSMRIAR"
     gene            4610..5434
                     /locus_tag="PLW25_02880"
     CDS             4610..5434
                     /locus_tag="PLW25_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53464.1"
                     /translation="MNPAVIQVNDLSFSYNPAGNAAVHQVSFEILPQTVTAILGPNGA
                     GKTTLLHLLLRLKTPLTGSIHLQGRPLNHYSRHELSQNIGLVPQTEYIPFEYTALEYV
                     TLGRAPYLGPLEIPAEKDTEIARQSLKEVGVSHLENRPIPALSGGELQLVLLARSLCQ
                     QPRVLLLDEPTSHLDLANRNITLQIMDRLRRAGTTLVFSTHDPEAAALIADKLVLMRA
                     GQVLETGSLEEVFTTETLSRTYGTRVEVLQVDGLRVVKSIERLGGEQNGQNNPAER"
     gene            5406..5933
                     /locus_tag="PLW25_02885"
     CDS             5406..5933
                     /locus_tag="PLW25_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOS53465.1"
                     /translation="MAKIILLSAEKGSGKTTALLKLREKLTGLTLNSRGVLSPPVMVN
                     GEKIAIELMDARSAERKRLAVPAALDSPEASGLRWHFFEDTLDWGNHLLSEAVPCDIL
                     FVDEMGPLEFDRNEGLTKGFFAIDSRRFLVALVTIRPALLERALARWSDAQVCKVTIK
                     DQPHLVNNLFRLITG"
     gene            5996..6817
                     /locus_tag="PLW25_02890"
     CDS             5996..6817
                     /locus_tag="PLW25_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOS53466.1"
                     /translation="MNPISELLSKVKTLFPKKVIGLALSGGATFGAAHVGVLQVLEEN
                     GIRPHLVAGTSAGALIGAAYCAGIPLSEIETLFRTMNWPTLLRLSIRNSLSIFDTQPM
                     EEFLRKKIGDIEFQELRIPFAAVACDIHTGARVILDRGALAPAIRASAAIPGLFSPVE
                     VNGRLLVDGGIVDNLPVEQVRDMGARYVISSDVSHRGPNSKKPENLFEIMLSMIYIMQ
                     SRAALPLEDASDCYIRPQVSQYSSWGFKDVPQVMEAGRKAARLVIPQLRRQLRIR"
     gene            complement(6924..8075)
                     /locus_tag="PLW25_02895"
     CDS             complement(6924..8075)
                     /locus_tag="PLW25_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOS53467.1"
                     /translation="MAQTIDAFARYDRRRERGVPTYLQEKWNGLSELNRFLAGSENLE
                     ELAVSASRRIVEILEVDFCQILLMDLDGQCNCRARYSAREMLIKKKASLDPIEIENIC
                     HRFLDAENNTRLFLGMEHLSERERKSLGISNQDISWIIPMRVETNPIGVLILGQSASQ
                     QEQILTPDSYYLVDLIADQLASAVFRAQMNERIESVSIETVIVLSKALETRDLHSASH
                     SKRMAALSEQLAKEFGFSARETRELCWAALLHDIGKIGVKDTILKKKGPLTEEEWAII
                     KTHPEIGAQIVRGVTGLEKIAPLILAHHERMDGKGYPNGLRGDEIPLGARILAVTDAY
                     TTMTDGRPYQKKRKHQEALQELLEQAGSAYDREVVQLFIQLFNKKDSLE"
     gene            complement(8152..8436)
                     /locus_tag="PLW25_02900"
     CDS             complement(8152..8436)
                     /locus_tag="PLW25_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53468.1"
                     /translation="MSNIFHDYHHHDETVVQKEREYSRILTAAVVNEKFRRLLLTNPN
                     MAIKKGFAGEAFHLGAEETRRISAIRVSTLAEFARQMNAEMTRPSLNLAE"
     gene            complement(8446..9021)
                     /locus_tag="PLW25_02905"
     CDS             complement(8446..9021)
                     /locus_tag="PLW25_02905"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HOS53469.1"
                     /translation="MILVVAVLVGLAAGLCRAKYGKREYRGYDLKAAGLVLLAFIPQG
                     FIFFLPSTRTRIPDETASFLFIISLVVLIIFSLFNIRKPSFWPITGGFLLNALVILLN
                     GGWMPISPEMVQRLLPDSTAGDWMVGERLGYSKDIVLLPENTRLWFLSDRFSLPDWIN
                     YQVAFSPGDIILFVGIVWLLWSLGGPKSVNV"
     gene            complement(9528..12764)
                     /locus_tag="PLW25_02910"
     CDS             complement(9528..12764)
                     /locus_tag="PLW25_02910"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOS53470.1"
                     /translation="MPPDLPVIRTKTLIPRRRSEILSRPRLLAALDNLLDLKLLIVAA
                     PAGYGKTSLLVDFSHHTQIPVCWYALDSLDSDPKRFIAHFIAAIQGKFPAFGQMASAA
                     LAELSQDNLNLDLVISAIVNDAYENISEHFIFVLDDYHLVRDVKPIEVFINRIIQESA
                     ENCHYVIASRTLLTLPDLSLLVARSEVDGLSFEELAFLPEEIKQLMAVNYHQSISDQK
                     AEEMITRTEGWITGLLLTAQVSPKGPYERSRIEKVSGVGIYEYLTQQVFDRQPLAMQS
                     FLLRTSLLEEFDANLCEKIVGKALGLEGQNWDEVIEKIQRDNLFVLPVGDETLFLRYH
                     HLFRDFLQNRMRVEKPEETLRIETELVAYYESVQEWERAIAILLRNGTPEQRVEMVRK
                     AAPAMILSGRLVTLTDWLNALPFDLVENKPELVSIRGTIAMLRGDYEVSLELLSRAIK
                     ELRAVGFAEELAAALIRRSAVNRHTGHYENALKDAQDAIELTRPPFGAPQKYAEALRA
                     EGLVYLQTGELNDAQWKLRESYRRFQDLGLESDAAKVLMDLGVIYSARADFEQSEKCY
                     STSLEYWQAVQNPLWLANVLNNIGMIQYLRNQFEQAVITLERSLSYSRLASPRIEGYV
                     LTSLGDLYRDVRALEEARQAYRQAEEVLQKTSDLSLDVYLTLALATLDRITGYLSSAQ
                     EQISAALEKAQKSGSKYEHHLCLLEQAILDFKNQKPADLAHTFEQLEDYFTAAGYQME
                     RFKAAVYRNLASLVETARTHTKLVIDPEMQKSEGSMRQVLIQILMEFGSYLELINPED
                     EQYPVVTQLKSEVAAFEKHLAQIHRVVRRHSSAVDFSNPKLSISGLGRMQVRLGNRVV
                     TNKDWKTHTVRDLFFFLLSHPEGATKEEVGAAFWPDADRDTVKLRFKNSIYRLRRAAG
                     SDCIAFDDDIYRFNRSMDYEYDLDTFMNELENAKKAQSTEEKIEHLRAAVSCYHGPFL
                     PKLDQEWVLIAREKFHTTFLNAAQALINLYMKVGKYSQAATLANRALEEDNYNESAYR
                     SAMLAYSAMNDRSAVARQFERCKLVLKKELDIEPSPQTVKLFHSLMQQ"
     gene            complement(12922..13299)
                     /locus_tag="PLW25_02915"
     CDS             complement(12922..13299)
                     /locus_tag="PLW25_02915"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HOS53471.1"
                     /translation="MDYNYDGGLGFGYYLFLLLVSVFSIVCLWKVFVKAGKPGWAAII
                     PIYNIITELEIIGRPWWWLLLLFIPLVNIVIGIIVIFDLAKVFGKSTGFGFGLLFLGV
                     IFMAILAFGDAKYLGPIAGQPAV"
     gene            13392..14186
                     /locus_tag="PLW25_02920"
     CDS             13392..14186
                     /locus_tag="PLW25_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOS53472.1"
                     /translation="MSIFLSKFLPLFLYPAGLVTLLILLTLVFWKKRRAAFTFLVLAF
                     FILLLAGNKYVATAFARTLEWRYPPLAENTTADVIVVLGGGTEPAIEPRRMVELNAAA
                     DRVLYGIKLYQEKAAPMLLLSGGDIDFIGDSPSTPADDMATLMEMLGIPREKMIIQNA
                     SLNTQQDAEFSCELIKKNGFKKVILVTSAFHMPRSVALFEKQGCPVVPAPVDFSITQL
                     SWDNLTHPTTAEFILDLLPSYSHISTVTKTLKEYFGIWYYKLSGVL"
     gene            14216..14941
                     /locus_tag="PLW25_02925"
     CDS             14216..14941
                     /locus_tag="PLW25_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53473.1"
                     /translation="MKPKFIALALISLLLVACSLPKSLTSIIQRFRSQPAAVAESNSL
                     LNLVENGKVSVVGIHGNVGLGTFTGRTIDLQLQNNTDSKLDVLIPCGLVFIADDEKSS
                     RMMVVQPTVTSLNKGKTAVVKPFVLSIDALKGLPSPEKTYSVAELEEGKNKDFATCLC
                     EQDLPAETETKDLLDLQLAAWMVSGEGLLSNIPVDLNDLFKDFSGLPITIPGLDEVMK
                     DVAGNVAPNAQAWLDKCGISFGK"
     gene            complement(15001..16146)
                     /gene="phnE"
                     /locus_tag="PLW25_02930"
     CDS             complement(15001..16146)
                     /gene="phnE"
                     /locus_tag="PLW25_02930"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HOS53474.1"
                     /translation="METLKSLLHSLKRVLIIIAVIALLVLSIRVTKPDLVKLVSSFPK
                     AKSIMGQLLRPEILGRESEKITVAQLVPVPCGSVANPEVPASGPRITVDPPCGNPKDK
                     VQVRGYELPKNAELSIRWILPDGGLLSIKNAVTNANGELTETMELRPITAAVDGKPAQ
                     LAFEITLPEGKVVASQTLKDVLDAMFVTIFMALIATTIGTIFAVPLSFLAASNITRKG
                     WLGSAVYYIVRAFLNIIRSYEPLVLATIFAMIVGFGSPFAGVIALSITTAASLGKMFS
                     ESVEGIDSGPIEAVTATGANKLQVVVYAVVPQIIPDFLSFTIYHWDINVRISTIIGFV
                     GGGGIGYFLSQRINTLQYSQSGTALLAIILVVWALDFLSSEIRKQLV"
     gene            complement(16148..17509)
                     /gene="phnE"
                     /locus_tag="PLW25_02935"
     CDS             complement(16148..17509)
                     /gene="phnE"
                     /locus_tag="PLW25_02935"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HOS53475.1"
                     /translation="MKEKTSRSRLVSIFLSALVPGLGQLIQKKYRAAIAIFLAVATSI
                     AVVAWYGKPAWYILPALIWLWNIWDASSLPGGKNAVLPILLWLLMAYGIGGQVTEFNL
                     RALVENSKRSKDIVSAMFQLDFTQPKTEKKEGFTDIQSPCSATPPVPDRTVNGVRVYV
                     VEPCATVNQTITVKGEGFWPNTPTLIYWQNPIGGKAMDAQVKSDAQGNVSLEWVVSPY
                     VHSTAPDPTVPQIHRIKVSQSRPIGGIELSDVGGYVVQGIYETLALAFLSTIVGALIA
                     IPFGFLAARNLMTGNPVTTAIFLVTRTILNIVRSIESLIIAIIFVIIVGLGPFPGMIA
                     ITIHTVAALGKLYSEVIEGIDSGPIDAVKATGASWPQVVRYAVIPQVVPTFTALTIYR
                     WDINIRSSTIIGFVGGGGIGFFLWQWIVLQDYRAVGTAFVAIAVVVIILDFFSARIRE
                     RLV"
     gene            complement(17515..18282)
                     /gene="phnC"
                     /locus_tag="PLW25_02940"
     CDS             complement(17515..18282)
                     /gene="phnC"
                     /locus_tag="PLW25_02940"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HOS53476.1"
                     /translation="MLKVEHLTKVYPNGTVALKDLSFEVPDGEFLVIIGLSGSGKSTL
                     LRCINRLIDPTEGKITMDGIDITAAKGAELRHIRRQIGMIFQHFNLVKRSSVMTNVLS
                     GRLGYLNSWSSTLGYFPHEEKERALAAMTRVGIADKVHSRADALSGGQQQRVGIARTL
                     MQEPKLILADEPVASLDPVLAHSIMQYIEKLNEEDNVSVICSLHYLDLIQRYAERVIG
                     LKDGVKVFEGLPSEINRAKFKEIYGEEAQDMRATALV"
     gene            complement(18355..19407)
                     /gene="phnD"
                     /locus_tag="PLW25_02945"
     CDS             complement(18355..19407)
                     /gene="phnD"
                     /locus_tag="PLW25_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOS53477.1"
                     /translation="MKKAMFVLISVLVLSTLVLSACAQPTAAPTKAPVATEAPAATEP
                     PAPQPTEAPKELGTAENPIIIALAPSATAEQLTVGGEAIAAKMKELTGLTYSVIIPNS
                     YTALIEAMASGNAHIGFMPTLAYLLGKQAGAMDVKLVVVRNGSDFYGAQFIAHKDMGF
                     TPYFDPNTNTDTADAATALAQFEGKRPCFTDPLSVSGYVIPFGVLKEQGVNFKTPAAV
                     QSHPAVVQALYAGVTVSGDKKVGAICDFGATYIDARTSDKIHTDVNEKIVVIWRTDNI
                     IPNDNISFSSAMPEEIGAKVYDGFVAMAADEAGKEIFKNAGYEIEDLKPAEDSFYDAF
                     RSALQASGVDITTMVK"
     gene            19953..20846
                     /locus_tag="PLW25_02950"
     CDS             19953..20846
                     /locus_tag="PLW25_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOS53478.1"
                     /translation="MINPHPGLPELEYVKPGTLAEACKFLEQHEGKARPFSGGTDCFV
                     RLRDGVLKLEYLVDIKGLDGTKELSFDPKKGLTIGAAITMNQVIAHKDVVKHYPNLVE
                     AAKSVASYQLRNRATIIGNICNASPAGDTIGTCLVLDGVLNVHGTGGKRTIPLNTFFL
                     APGRTVLKPGEVVTSITLPPPPAGMAGVYKKLGRNKMSDLSIVGVTVMGYPNSQSPSG
                     FSFRIALASVAPVPMEALKAEAILAEKKITAESIALAAEAAMEAVTPIDDVRGSARYR
                     KYMVRNLTREAITEVWNTLTK"
     gene            20879..21343
                     /locus_tag="PLW25_02955"
     CDS             20879..21343
                     /locus_tag="PLW25_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOS53479.1"
                     /translation="MSYSKITLTVNDVVETVDVPHHMTLLQMLREKLSLTGTKNGCSA
                     GECGACTVLMNDEPVNSCMVLAAECDGARIVTVEGLAKSGKLSKLQEAIIKTGGVQCG
                     FCTPGMLISATALLKRNPNPTEKEIREALVGNLCRCTGYFRIVEAVKEAAHS"
     gene            21401..23758
                     /locus_tag="PLW25_02960"
     CDS             21401..23758
                     /locus_tag="PLW25_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOS53480.1"
                     /translation="MANAKSTEKTTAVVGTSVIRNDVLEKVTGAATYVDDIQFGNKLL
                     YARAVRSPHPHALIKHIDTSKAEKLPGVKAVVTGKDFPERIGLYLKDKTIFATDRVRF
                     LGEPVAAVAAVSEEIAEKACSLVEVKYEPLEGVFDAEYGASEKAPVIHPDLGKYDCVN
                     FIFPKPGTNISNHFKIRKGDVEAAWKQCAHIVEKTFKVPHIQHVPIETHGAVAMMDEK
                     GKVTVWAASQSPFAQRNLIAKALHISHSDLRVIAPLVGGGFGSKAGLTMEAIPVALAT
                     KVKGYPVKFILTREEEFYTNFVRQGLTIHVKVGCDKDGNILGLKNVMYWDGGASTEYG
                     TNITRAGGYSSSGPYDIPNVWTDSICVYTNHPIGGAYRGFGMSELHTGIDQAIDELAE
                     VAGFDRVEFLKKNAISEGDIVVTGMTMHATGIQQCIDNVAKAIEFSKKEEPSSPDRLR
                     GKGIAIMWKAPAMPPNAGSSAWVELSEDGKATVGLGGQEIGQGTFTVMAQIAAETLGV
                     KYEDVRIAGPVDTQYSPYEWQTVASRLTWSMGNAVRLAALDARKQILDVVAEAWNETP
                     EDLDIVNGMVVSYKSEQETPLANLVIYGLPKPNDEGWMGGPIVGRGSFMPKYVTNLDA
                     ETGQGARAVVHYTVGCEGLDIEIEKNTGRIHVIKAAASFDVGKAINPALVKAQVEGGF
                     VQGLSSAMFEEIRLRNGIMTNPSFVDYRIATAADADFKLDTQYVEVPQDDGPWGARGV
                     GEHPMVPTIAALGNAIYDATGVRLQGPPYSAESIYLGMLNAGVVK"
     gene            23820..>24989
                     /locus_tag="PLW25_02965"
     CDS             23820..>24989
                     /locus_tag="PLW25_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS53481.1"
                     /translation="MTDHYDLIIRNATLRNEPKSTQDIAIQDGKVALIANKIEAKAAS
                     ELDARGNLVTESYVNTHLHMCKVYTLMMMDEAALKDYHGADMGKAMTAIELAARVKEK
                     YDEKWIIKNVRKAAALAARYGCTHIRAFADVDSKGKLEGIKALIKARDEFKGIVDIQT
                     VAFAQDGLVREPGADKLMRQAMDLGADVVGGIPWIEYTDADIAEHVRICYQLAKEYDK
                     PVSMLVDDAGDNGLRSLEVMALETIRTGWQGRALAHHARAMALYPKPYLQKLAAILKK
                     ADMGVVSDPHTGPLHARVRELLEEGVLVCLGQDDISDAYYPYGRNNMLEVAFLNSHLL
                     WFTTREDMEVLYDMITTKPARAIGLKDFELKKGAPANLVVLDQPNVLEALRYHEKP"
BASE COUNT         6072 a         6693 c         6393 g         5831 t
ORIGIN      
        1 ggtgatcaaa cgggtgaaga acaggctcca tttccagggc gcaactatcc acctgcccca
       61 taacaggcgg gtgagcagcg atggtagacc gaaataatgt ccccattcca gcacgcgcag
      121 cgcgcgcggg gagaaataat cccagcagtc caccagctca aaccctgcac cttccaggcg
      181 ttgtcgccag gtagagggat catcacagtg atgatgccgt gaaatgcggt taaaaaaacg
      241 ccggtaaagg tcacctaaaa atcgcaggcg caggttatcg aagaaacggc caatggagag
      301 cgtacccaaa aagcgctgat tgggcacaca aaaaacgaac gccgctcccg ggcgtaacac
      361 cctccccagt tccgcgagca caggttcaag cgccgggata tgctccagca cggaattgct
      421 caccgcgctg gcaaaatacg catcagggta cgggatctcg ctcgctttcg tcagtaccgc
      481 ctgctgatac acaccgcgcg cttcggcttc acgtaaaggc ttctcccagg ggtccaggcc
      541 aacctcgagc gcacggttaa aagccagaga ggcgaagtgc ccatcaccgc agcccaggtc
      601 cagcgctggg gcaggcaggt caatgttctc atagaatctc gcttccaccg cccttaaaag
      661 agcgcgaaag tacggcaacg agctgatttg cagccacagg tggtctttac cggcctggct
      721 ggcttcaacg ggtgtgtgtc gctgcaaggt cacgcttgct cctgaaaaat aacctcatca
      781 ttcttgaaac acggcgctta tgtattaccg cgcctattat aaacgttttg aaatggaacg
      841 aaagttcccg tcaactcaat gagctggcgg gaaccagtat ttgggcgcaa agatagaaca
      901 ggtctgtaaa ttattggctc atttgggcga gggcttttaa gagagagttc gccaccagag
      961 tgccagattg ggtgcgataa ttttgcgtgc ccatacgact tccagccatt aaggcgctta
     1021 aaagggcgtt gatgtcactg ctgccggatt gtttggcctg catgtaagcc ataccggctt
     1081 tcagcagctg cgcggtgtcg atcttaaccg gttttgaacc gcttggtgca gcagcgcccg
     1141 ttaatccgcc gaggaggctg cttaacaggt catcacctgt cccggcgcct gcgggcgcct
     1201 gttgcgcgcc ggcaagtccg ccgagcagcg agcctaagag gtcgccgcct gcgccagcgc
     1261 ctgaaggagt ctgctgcgcg ccggcaagcc cgccgagcag cgaacccaac aggtcgccgc
     1321 ctgctgattg cccggagggt tgctgttcaa aggtttttct ggcttgcgtt tgcagtgttt
     1381 gcggggtgac ctttttctgg gttcccatta acgcctgaag caaggtcagc gcgttgttgg
     1441 tggtgagggt cttcttgccc cccagctgag cagaggcgtc ttgcaatccc tgagcataaa
     1501 gttttgcgga gctgctctga ctcttggtct gcagcagctg gctggcgtaa gccagttgtt
     1561 cagctggtgt ggcgttgctc ttcgctttca tcgcctgggt gattacctcg aagatctcca
     1621 ccatgttatc gccgtgattg tggttgtagg tatccgcctg gtttaaagcc gccttatgct
     1681 ggtccaatgt ggaaaccacg ctgccaaaaa gttgcgccag atcaatactc tggtttgtca
     1741 tgagaatttc ctttccctgt ttgtttttgt ctgcacatta tatcctggtt ttgttgacaa
     1801 acgaaattca gtatgttatg atttacttat tcaaatattg aatattcatc ctatgaacga
     1861 taaaaaactc ccactcaacg ctgctgaata cgccctgctt ggtttcctgg tggctggacc
     1921 gtgccatggg tacgagctgc accgaaagct cacagaacca gacggcgcgg ggatgatctg
     1981 gggaatcaag atttcaaata tgtacgccca actggataaa ttggagcggc gcgggttgat
     2041 cagcggtcgt ttgttggaaa acgaacagcg cccagcgaga acgcagtact cgctcaccgc
     2101 tgccggaaaa caactgttcg aagagtggct gcatcaacca gtgcagcacc cgcgtgattt
     2161 ccgccacttt ttttttctgc aattattctt tctctctcag tacgagcctg acgcgcttga
     2221 atctctggtg gagcgccagc tggagctctg ccaggcgtgg cagcaaaatt tgttggcagc
     2281 gcaggagagg tcttccacct ccgttgattt cgctgacctg accaatcgtt ttcgccactc
     2341 acagatccaa tccatgatcg attggttgtt ctggatcaag agtgaaaaaa tcaaaatcaa
     2401 accaaagcga ggtaatcaat gaaacgtccc cttcaattct tagtggtact tgttctgttc
     2461 agcagtttac tggcagcatg cgcggcgccc acgcccgccg ccgcaccttc tctcactgaa
     2521 cccccagcca cagaagctcc ggcagccgtg agcacgccag ccccaaccga gccaccggag
     2581 atggaaaaaa tcacggtcgt agacgcgctt ggacgggaag ttgtatttga ccaacccccc
     2641 tcgcgcatca tgatcgtggg caaggcagcg ttcatgttgc ttgacgcagc tttcctcttc
     2701 cctgaggctc ctgaaaaaat catcgggtat gagctgcgtt ctcaaaccgg gctggatttc
     2761 atccacacgg cttttcctca aagccataac atgatcgtgc ttgagatgga cgctggaccg
     2821 gaacagatcg ccccggcaaa ccccgacctg gtattgatga agtcatacct taaagaacaa
     2881 ctgggagacc aggttgagct gcttggcatc aaggtggtgt accttgatct ggaaacgccc
     2941 gaaacgatca accaggatat ccgcaccctt ggaaaactct tcggtaacac tgagcgcgct
     3001 gagcaattgg tgaccctcta tgaccaggcg cgcgcgaaaa tcgccgatat caccaacgtc
     3061 atccctgaag aagaaaaacc ttccaccctg cttctccaat attccgataa aggtggcgaa
     3121 gtcgctttta gtgttcctac aggcggcttc ttgcaaacac aattggtcga aatggccggc
     3181 ggggcgcctg tctggaagga agtgacggga aagggctggt ctgtcattac actggagcaa
     3241 atcgccgcct ggaacccgga gatcatcatc gtcgttgatt accgcggaaa agccatggag
     3301 gccgttgacc ggctgaaagc agatgaaaac tggcagcaac tggacgcggt aaagaatgag
     3361 aaactctacg cctttccatt ggactttcag agctgggatc aaccagattc acgctggacc
     3421 ttgggcctga cctggctcgc aaccattatg cagccagagc gttttgcatc cgtgaaaatg
     3481 cacgaagaaa ttccggcctt ctacacggat ttctacgggt taagcgaatc tgtgatcgag
     3541 cagagcattc tgccgttggt gaagggcagt tttttagagt aagcgggtaa taaaaaatca
     3601 cccggtttgt gcaggcagca ttgaaaaaca acaaaaacct caccgccttc gtgctcgtgg
     3661 ttacgccgct gattgttctt ggtatctctt tattcatcgg cagataccca aaaccgctct
     3721 tgatgcccct gtctgtgttg ctgcacgacc agctggggca aaacctggtt tacaatctga
     3781 ggttaccacg cctgatcacc tccgccctgc tgggcatgtc actggccacc gccggaggtg
     3841 tgatgcaaat gcttttccgt aacccgttgg tagaaccggg gttcctgggg gttactcagg
     3901 gggcagggtt tggcgccgcc ttgagcatcc tcttccttac aaccgcgccg ctggtggtag
     3961 aactgagcgc cgctggtttc gccatcatcg gcttgctgct ctcttatttc atcgcccggc
     4021 gaatccgttt tggcgggtgg gtgctgcggc ttgtgctctc aggtatcgcg gtttcagcca
     4081 tcttttcagc ggggcttggt atccttaaat atatggcaga ccccacctct cagctgccag
     4141 agatcgtgtt ctggctgctg ggaggtttgt ggtctgtcac ctggacggat ctatggtata
     4201 tcctgcctgt ggtggttatc ggtctggtca ttatctacct catgcgctgg cgcttgaacc
     4261 tgctttcgtt gagcgacgag accgcttttt cgttaggagt ggcggtaggg cgcgaacgca
     4321 ccttgctgct ggcagccagc gtggcgatta ccgctgtggt ggtttccgta gcgggcatcg
     4381 tgggttggat cggtttgatc atcccccaca ttgccaggcg catcgccggt gccaacgcgg
     4441 cctccttcat accggtgagc atgtcattag gcgcagtctt tggcatcatc tgcgatgacc
     4501 tggccagggt gctgaccacc ggcgaagtgc cgctgggaat tatcacctcg ctcatcggcg
     4561 ctgtcatctt cttgatcatg atgacttctc gttccatgag gatcgcaaga tgaacccagc
     4621 ggtgatccag gtcaacgatc tgtccttttc gtacaacccg gcaggcaacg cggctgttca
     4681 ccaggtttcg tttgaaatcc tcccccaaac ggttaccgct atcctggggc ccaatggcgc
     4741 aggaaaaacc actttattgc acctgctctt gcggcttaaa actccgctca caggctccat
     4801 ccacctgcaa ggcagaccac tcaatcatta ttcccgtcat gaactcagcc agaatatcgg
     4861 cttggtgccg caaacagagt acatcccatt tgaatatacc gccctggagt atgtaacgct
     4921 cggcagggct ccttacctgg gcccgctaga aattcccgct gaaaaagata ctgaaatcgc
     4981 ccgtcaatcc ttgaaagagg taggggtgtc gcatcttgaa aaccgcccca tccccgccct
     5041 gagcggtggt gaactgcaac tggtgttgct cgcccggtct ctatgccagc agccccgcgt
     5101 gctattgctg gatgaaccca cctctcacct ggatctcgcc aatcgaaata tcaccctgca
     5161 aataatggac cgcttgcgca gggcaggcac gaccctggtc ttcagcacgc atgaccccga
     5221 agccgctgcc ttgattgccg acaagctggt tttaatgcgc gccgggcaag tcctggaaac
     5281 cgggtcgttg gaagaggtgt tcaccacaga aacgctgagc cgcacatacg gtacgcgggt
     5341 ggaggtgctc caggtggatg ggttgcgtgt tgtaaaatcc attgaacgtc ttggtggaga
     5401 acagaatggc caaaataatc ctgctgagcg ctgaaaaagg cagcggtaaa accaccgcgc
     5461 tccttaaact gagggagaaa cttaccggcc tgaccttaaa ctcacgcggg gtgctgagcc
     5521 ccccggtgat ggttaacggg gagaagatcg ccatcgaatt aatggatgcc cggagcgctg
     5581 aacgcaagcg actggcagtg ccagccgcgc ttgattctcc agaagcatct ggactaagat
     5641 ggcatttttt cgaagatacg ctggattggg gaaatcacct tctttcagag gcggttccct
     5701 gcgacatcct ttttgttgac gaaatgggcc cgctcgagtt cgaccgaaac gaggggctga
     5761 ccaaggggtt cttcgccatc gactcgcggc gttttctggt ggcgctggtg accattcgcc
     5821 ccgcgctgct ggaaagagcc cttgcgcgct ggtcagatgc ccaggtctgt aaggtgacta
     5881 ttaaggacca acctcacctg gtcaacaacc tgttccgcct gatcactggt taaccgtatt
     5941 ttgccccagc cgcagtttga aatttttacg ccaatcccac gtatacaata ttgatatgaa
     6001 cccaatcagc gagttgttga gtaaggtaaa aaccttgttt ccgaaaaagg tcatcggcct
     6061 ggccttgagc ggcggcgcca cctttggcgc tgcgcacgtg ggcgtgctgc aagtacttga
     6121 agaaaacgga attcgcccgc acctggtcgc aggtaccagt gccggggcgc tgatcggcgc
     6181 ggcttactgt gcgggtatcc cgttgagcga gattgaaacc ctctttcgca cgatgaactg
     6241 gccgacattg ctccgccttt ccatccggaa ttcactctct attttcgata cacagcccat
     6301 ggaagaattt ctgcgcaaga agattggtga tatcgaattt caagaactca ggatcccgtt
     6361 cgccgcggtc gcctgtgaca ttcacaccgg cgcaagggtc atcttagacc gtggcgcgct
     6421 cgcgcccgcc atccgtgcca gcgctgccat tccaggcttg ttttcaccag ttgaggtcaa
     6481 tggccgtctg ctggtagatg ggggaatcgt ggataattta ccagttgaac aggtgagaga
     6541 catgggcgcg agatacgtga tttcaagcga tgtatctcac cgtggaccaa actccaagaa
     6601 accggaaaat ctcttcgaaa tcatgctgtc gatgatctac attatgcaat ctcgcgccgc
     6661 cctcccgctt gaagacgcat cggattgtta cattcgtccc caggtgtctc aatactcatc
     6721 ctggggcttc aaggatgtcc cgcaagttat ggaggctgga agaaaagccg ccaggctggt
     6781 tatccctcaa ctgcgccggc agttgcgtat ccgctaacac gcgcgccgga taaagaaaat
     6841 ggatggccca agcctacggc catccatttc agatttcccc ctttagcgaa gaacaggtat
     6901 gccttgcaaa ggcaataaag aaattactcc agactgtctt tcttattaaa taattgaatg
     6961 aataattgca ccacttcacg gtcataggcg cttcctgcct gttcaagcag ttcctgcagt
     7021 gcctcttgat gcttgcgctt cttttgatat ggccgtccat ccgtcatggt tgtataagcg
     7081 tcagtcacag ccagtatacg cgcccccaaa gggatctcgt caccacgcag accattgggg
     7141 taaccctttc catccatgcg ctcgtggtga gcgaggatga gcggcgctat cttttcaagc
     7201 cctgtcaccc cgcggacaat ttgcgcgcca atttcaggat gggtcttaat gatcgcccat
     7261 tcttcttcgg tgaggggtcc tttctttttc aaaattgtgt ctttcacgcc aatttttcca
     7321 atatcgtgca gcaacgctgc ccagcacaat tctcttgtct cacgggcaga aaaaccaaac
     7381 tctttggcca gctgctcaga aagagctgcc atgcgcttgc tatgagacgc gctatgcaga
     7441 tctcgggttt caagcgcctt tgataaaacg atgacggtct caatagatac agactcgatg
     7501 cgctcattca tttgggcgcg aaagaccgcg ctcgccagct ggtcggcaat cagatccacc
     7561 aggtaataag aatcaggcgt cagtatctgt tcttgctggc tggcagactg ccctaaaatg
     7621 agcacaccga ttgggttcgt ttctacccgc atgggaataa tccaggatat atcctggttg
     7681 gatattccca ggctctttcg ttctctttcc gataagtgtt ccatccccag gaacagcctg
     7741 gtattgtttt cagcatccaa aaacctatgg cagatgtttt ctatctcaat cgggtccaga
     7801 gaggctttct ttttgatcaa catttcacgt gcggaataac gcgcgcggca attacattgc
     7861 ccgtcaaggt ccattaaaag aatctggcaa aaatccactt ccaggatctc aacgatacgc
     7921 ctggaagcgc ttactgccag ttcttccaga ttctctgaac ccgccaggaa gcggttcagt
     7981 tctgaaagac cattccattt ttcttgtaga taagttggga cccccctctc cctgcgtctg
     8041 tcataccgcg caaatgcatc aattgtctgt gccatgtctc ttaccctgtt gatggggagt
     8101 caataatgac gaacgatcgt ttcgttcgta gaaaattagt tggttgtcta tctactccgc
     8161 taaattgagc gagggccggg tcatttctgc gttcatctgg cgggcgaact cagccagggt
     8221 ggaaacgcgg atggcagaaa tcctgcgggt ttcttcggcg ccaaggtgaa acgcctcacc
     8281 ggcaaaaccc ttcttaatcg ccatgttggg gtttgttaat agcaatctgc ggaatttttc
     8341 attcacaact gctgcagtga gaatgcggct gtattcgcgt tctttttgca caacggtttc
     8401 atcgtgatga tggtaatcgt gaaatatgtt actcatttta ttctcctata catttacact
     8461 ctttggtcca cccagcgacc agagcagcca gacgattccc acaaagagga tgatatcgcc
     8521 tgggctgaac gccacctggt agttgatcca atcgggtaaa gagaagcggt cgctcagaaa
     8581 ccacaagcgg gtgttctcag gcagcaagac gatatctttg ctgtagccga ggcgctcgcc
     8641 caccatccag tcgcctgctg ttgaatctgg cagcagccgt tgcaccatct ctgggcttat
     8701 cggcatccag ccgccattga gcaagatgac cagcgcgttc agcaaaaaac cgccggtgat
     8761 tggccaaaaa ctgggtttac ggatattaaa gagcgaaaat atgatcagta caaccagtga
     8821 aatgatgaaa aggaaggaag ccgtttcatc ggggatgcgg gttcgggtag atggcaaaaa
     8881 gaaaatgaag ccctggggaa tgaaagccag taagaccaat ccagccgctt taaggtcata
     8941 gcccctgtat tcccttttac catatttcgc cctgcacagc ccggcggcta aaccaaccag
     9001 tactgcgacg accaggatca tttattgtcg gctatccgcc aacagtaccg gtggcgaaag
     9061 gagcgcccgc cgccagggtg aacagcacca gcacgagcac gaagaagacg aaccttgctt
     9121 tgttggaaat ggttttcatg agagactcct tttttactct aatcattttg tcaggacggc
     9181 gctaaaagca cacaggcttg cagcagccag gacgattgag atggatctat ccgccaacag
     9241 tgccggtggc gaaaggagcg cccgccgcca gggtgaacag caccagcacg agcacgaaga
     9301 agacgaacct tgctttgttg gaaatggttt tcatgagaga ctccttaaat gattagtcgt
     9361 tttttttact ttacctgatg attatgcctg caggcaggat gcaaaacagg atgcataatc
     9421 gactcttaac ttcagggagc tatcctgact tgttaaacac caaaattcag agatggtctt
     9481 atattcacct ctttctcatt tgatcttcaa tttggctgga taaatgctta ctgttgcatt
     9541 aatgaatgga ataacttgac ggtctgtggc gagggttcaa tgtcaagttc ttttttcaat
     9601 acaagcttgc acctttcaaa ttgcctggcc accgcagaac gatcattcat ggcagagtag
     9661 gccaacatcg cagaacggta cgcggattcg ttgtaattat cttcctcaag cgccctgttg
     9721 gcaagggtgg ctgcctgtga atacttgcca actttcatat agagattgat gagcgcctgc
     9781 gctgcgttca agaaggttgt gtgaaatttt tcgcgcgcga tgagcaccca ttcctggtct
     9841 aatttgggca agaaaggacc gtgatagcaa gagaccgccg cgcgcaaatg ttcgattttt
     9901 tcttccgtgc tttgcgcctt tttggcgttt tccagctcat tcataaacgt atccaaatca
     9961 tattcataat ccattgagcg attgaaccgg tagatatcat catcaaaggc aatgcaatcg
    10021 ctgcccgctg cccggcgcaa ccggtagatc gagttcttaa accgcagttt caccgtatca
    10081 cggtctgcgt ctggccaaaa tgcggcccca acctcttctt tggtcgctcc ctcagggtga
    10141 gaaaggagga agaaaaacaa atcgcgtact gtatgtgttt tccaatcctt gttggtaaca
    10201 acccggttcc ccagccgcac ctgcatgcgt cccaaaccac tgatgcttaa cttggggttg
    10261 gagaaatcca ccgctgagga atgccggcgc accacacgat gtatttgcgc taaatgtttt
    10321 tcaaaagccg ccacttctga ctttaattgc gttaccacag ggtactgttc atcctccggg
    10381 ttgatgagct ccagatagct gccaaattcc atcagtattt ggattaaaac ctggcgcatg
    10441 ctcccttctg atttttgcat ttcagggtct ataacaagct ttgtgtgtgt tctggctgtt
    10501 tcaaccaaag acgcgaggtt acggtacacc gccgccttaa atctctccat ttggtagcct
    10561 gccgcggtga agtaatcttc cagttgttcg aaagtatgcg ccaaatccgc tggtttttgg
    10621 ttcttaaaat ccagtatcgc ctgttccagc aggcagagat ggtgttcata cttcgagccg
    10681 cttttttgcg ctttttctaa tgccgctgat atttgttcct gggcggaaga aagatagccg
    10741 gttatgcggt caagcgtcgc cagcgccagt gtgaggtaaa catccaaaga aaggtcgctg
    10801 gttttctgca acacctcttc tgcctggcga taggcctgcc tggcttcttc aagcgcgcgc
    10861 acatcacgat acagatcacc caggctggtc agtacatacc cttcgatcct ggggctggcc
    10921 aacctggaat aagatagcga gcgttccagg gtaatgacgg cctgttcaaa ctggttgcgc
    10981 aggtactgga tcatgccgat gttgtttaag acattggcca gccatagagg gttttgcacc
    11041 gcctgccagt attccagtga agtggagtaa catttttcgc tttgctcaaa atccgctcgg
    11101 gcggaataaa tcaccccaag atccatcaat actttcgcgg cgtccgattc cagtcccagg
    11161 tcttgaaaac ggcggtaaga ctcgcgtaat ttccactgcg catcattcaa ttcaccggtt
    11221 tgcaaataca ccaacccttc cgctctcaat gcctcggcgt atttttgagg agcgccaaag
    11281 ggtggtctgg tcaattcaat cgcgtcctgg gcgtctttaa gcgcgttttc ataatgcccg
    11341 gtatggcggt tgacagcgct gcgccggatc agcgcggcag ctaattcttc tgcgaagccc
    11401 actgcgcgca actccttgat cgcccggctc agcaattcca ggctgacctc ataatcacct
    11461 cgcagcatgg caatcgttcc cctgatagac accaactccg gcttattttc caccaggtca
    11521 aagggaagcg cgttcaacca atccgtcagc gttaccaacc gcccgctgag gatcatcgct
    11581 ggagcagctt tgcgcaccat ttcaacccgt tgttccgggg ttccattcct cagcaaaatg
    11641 gcaatcgctc tttcccattc ctgcacactt tcgtaataag ccaccagttc agtttcgatg
    11701 cggagtgttt cctccggctt ctctacgcgc atgcggttct gcaggaagtc gcggaagagg
    11761 tgatggtaac gcaggaaaag cgtttcatcc cctaccggca gcacaaacag gttgtcgcgc
    11821 tggattttct caatgacctc gtcccagttt tgcccctcta accccagcgc tttacccacg
    11881 attttttcac aaaggttggc atcaaattct tccagcaggg atgtgcgcaa tagaaagctt
    11941 tgcatggcca gcggctgccg gtcaaagacc tgctgggtaa gatactcata gatacccacg
    12001 cccgacacct tctctatgcg gctgcgttcg taaggtcctt taggcgaaac ctgcgcggtc
    12061 aacagcaggc cggtgatcca gccttcagta cgcgtgatca tttcctcggc tttttggtcc
    12121 gaaatggact gatgataatt caccgccatc aactgcttga tctcttcagg cagaaaagcc
    12181 agttcttcaa agctcaagcc atccacctca gagcgcgcca ccagcaggct caagtctggc
    12241 agagtgagca gcgtgcgaga ggcgatgaca taatggcaat tctccgctga ctcttgaatg
    12301 atgcggttga taaacacttc gataggttta acatcacgaa ccaggtggta atcgtccaga
    12361 acgaagataa agtgctcgct gatgttttca taggcgtcat tgacgatcgc tgaaatcacc
    12421 aggtccaggt tcaaattatc ctggctcaat tcagcgagcg ctgctgaagc catctgcccg
    12481 aaggcgggga acttcccctg gatggcagcg ataaaatgcg cgatgaagcg cttggggtcg
    12541 ctgtccagtg aatcgagcgc gtaccagcag actggtattt gagtgtgatg tgaaaaatca
    12601 accagcagcg atgttttacc ataaccagcc ggagcggcga caattaagag cttaaggtcc
    12661 agcaaattat caagcgcggc gagcagcctg gggcgcgaga ggatttcgct gcggcgtcga
    12721 ggtataagcg tttttgtgcg gatgaccgga agatctggag gcatatgtat ctactataat
    12781 ccagtctttg tcatgctggg cttaacgaat atgtctactt gaattatata caaaataaga
    12841 gtcctcttcg gactcttatt ttgtacgatt cgagaaatat taatttggct acattggtat
    12901 tggtgggttt atcggagggg tttacacggc cggttggccg gcgattgggc ccaggtactt
    12961 ggcatcacca aaggctaaaa tcgccatgaa aatgacaccc aagaagagca agccaaaccc
    13021 aaaacccgtg ctttttccaa atactttagc caggtcgaaa atcacgataa tcccaatcac
    13081 gatattcacc aggggtatga ataagagcaa aagccaccac cagggacggc cgatgatttc
    13141 caattcagtt attatgttat agattggaat gatcgcagcc caacctggtt ttccagcttt
    13201 tacaaatact ttccacaaac acacaatgga aaaaacgctg acaagcaaaa gaaagagata
    13261 gtacccaaag cctaaaccac catcatagtt gtaatccata cgtcctcctt tttaggttat
    13321 gattacgttg aattaatcat aacataattg tcaatatatt ggatatatat tatccatttt
    13381 gaaagggcaa aatgagcatc ttcttatcca agttccttcc cctcttcctg tacccagcgg
    13441 ggctggtcac gctgcttata ctcctgaccc ttgttttctg gaagaaacgc cgggcggcct
    13501 tcacatttct tgtcctggct ttcttcattc tgctgctcgc cggcaacaaa tacgtcgcca
    13561 cagccttcgc gcggacttta gaatggcgtt acccgccgct ggcggaaaac accactgccg
    13621 atgtgatcgt tgttctgggc ggcggcactg aaccagcaat agaaccgcgc cgcatggtgg
    13681 agttaaacgc cgccgccgac cgggtgcttt acggtatcaa gctgtaccag gaaaaagcag
    13741 caccaatgct cttgctcagc ggtggagaca ttgactttat cggcgattct ccctccactc
    13801 ccgctgatga catggcaacg ttgatggaga tgctcggcat cccgcgagag aaaatgatta
    13861 tccagaacgc ctcgctcaat acacagcaag acgctgagtt cagctgcgag cttattaaaa
    13921 aaaatggctt taaaaaagtc atcctggtga cctcggcgtt tcatatgccg cgctcggtcg
    13981 ccctttttga aaaacaaggc tgtccggtcg tcccagcacc ggtagatttc agcatcaccc
    14041 agctaagttg ggataacctc acccacccaa ccactgctga attcatcctc gacttgctgc
    14101 cttcgtactc gcatatttcc acggtgacca agactttaaa ggaatacttt ggcatatggt
    14161 actataagct ttccggggta ttgtaaacag cccgggttga aagaaggaga agataatgaa
    14221 acctaagttc atcgcgcttg cgcttatcag tctactcctg gtggcttgtt cgctgccaaa
    14281 atcattaact tccattattc agcgatttag atctcaacca gcagcggtgg cagaatccaa
    14341 ttccttgctt aacctggttg aaaacgggaa agtgagtgtg gtcggcatcc acggtaacgt
    14401 cggactgggc accttcactg gcaggacgat cgacttacag ctccaaaaca acaccgacag
    14461 caaactcgat gtgctcatcc cttgtgggct ggtattcatc gctgatgacg agaaaagcag
    14521 ccgcatgatg gttgttcaac ccaccgtgac cagtctgaac aaagggaaga cagcggttgt
    14581 aaagccgttc gtcctcagca ttgacgccct aaaaggttta ccctcgcccg aaaagacata
    14641 cagtgtcgct gaactggaag aagggaaaaa caaagatttc gccacatgct tatgcgaaca
    14701 ggacctgccc gcggaaaccg agaccaaaga ccttctcgac cttcagctcg ccgcctggat
    14761 ggtcagcggt gaagggcttc tgtccaatat cccggtggac ctcaacgacc tgttcaaaga
    14821 cttctccggg ctgcccatca ccatccctgg gctggatgaa gtgatgaagg atgtggccgg
    14881 gaatgtcgcg cccaatgcgc aagcctggct ggataaatgc ggaatatcct ttggcaagtg
    14941 attcacaaga ggctggtcgg taaccctgac cagcctcttt gatattcatt taaagaaggt
    15001 ctataccagc tgtttgcgta tctcagagct cagaaagtcc agcgcccaca ccaccaagat
    15061 gatcgccaac agggctgtgc cagattggga atactgcagc gtgttgatgc gctgcgagag
    15121 gaaatatccg attccacccc ccccgacaaa accgatgatg gtagagatgc gcacattgat
    15181 atcccaatgg taaatggtaa aagaaagaaa gtccgggatg atctgcggca ccacggcata
    15241 taccaccacc tgcagtttgt ttgcgccggt cgcggtgacc gcttcgatag ggccagagtc
    15301 gatgccttcc actgattctg aaaacatctt tcccagagaa gcggctgtcg taatggaaag
    15361 cgcgatgaca ccagcgaacg ggctgccaaa ccccacgatc atggcaaaga tggtggccaa
    15421 caccaacggc tcgtatgaac gtatgatatt caagaaagcc ctgacaatat agtaaacagc
    15481 cgagccaagc catcccttgc gggtgatatt ggaggctgcc aagaaactga gcggcacggc
    15541 aaagatggtg ccgatggtag tggcgatcaa ggccataaag atggtcacaa acatcgcgtc
    15601 cagtacatcc tttaaggtct gagatgccac aaccttgcct tccgggaggg taatttcaaa
    15661 cgccagttgg gcgggcttcc cgtcaaccgc cgctgtgatc ggtcgcaatt ccattgtttc
    15721 tgtcaattca ccattggcat tcgtcaccgc gttcttaata cttaacagcc cgccatccgg
    15781 caatatccag cgaatggaca gttcagcgtt cttaggcagt tcatatcccc ttacctgaac
    15841 tttgtccttg gggttaccgc agggcggatc gacggtgatt cgcggcccgc tggccggcac
    15901 ttcagggttc gcgacgctgc cgcagggtac cggcaccagc tgtgccacgg tgattttttc
    15961 cgattcgcgc cccaggatct caggtcttaa caactgaccc ataatcgatt tcgccttggg
    16021 gaaagaagaa accagcttaa ccaggtcggg tttggtcaca cggatactga gcaccagcag
    16081 cgcgatcacg gcgatgatga tgagcacccg tttgagtgaa tgcagtaacg atttcagcgt
    16141 ctccatgtca caccagccgt tccctgatac gggcgctaaa gaagtccagg atgatcacaa
    16201 ccaccgcgat ggccacaaac gcggtgccca cagcgcgata atcctgcagc acgatccact
    16261 gccacaggaa gaacccaata ccgccgccac cgacaaaacc gatgatcgtg gaagaacgga
    16321 tattgatatc ccatcggtaa atggtcaacg cggtaaatgt cggcaccacc tgaggaataa
    16381 ccgcgtagcg taccacctgc ggccagctgg ctccggttgc cttcaccgca tcgatcggtc
    16441 cgctgtcaat cccttcaatt acctctgagt agagcttgcc cagcgccgcg acggtgtgaa
    16501 tggtgatggc aatcatgccc ggaaaaggtc caagacccac gataatgaca aagatgatgg
    16561 cgataatcag agactcgatc gaacgcacga tattgaggat tgtcctggtg accaggaaga
    16621 tcgccgtggt cactgggttg ccggtcatca agttgcgggc tgccaggaag ccaaacggaa
    16681 tggcaatgag cgcgccgaca atggtggaca gaaaagccag cgccagcgtc tcgtaaatac
    16741 cctgcacgac atagccgccc acgtctgaaa gctcgatacc gccaatgggg cgagactgtg
    16801 agactttgat gcgatgaatc tgcggcactg tggggtccgg cgcggtgctg tgaacatagg
    16861 gagagaccac ccattccagg ctcacattcc cctgcgcatc tgatttaacc tgcgcgtcca
    16921 tcgctttacc gccaataggg ttctgccagt aaataagagt gggcgtgttc ggccagaaac
    16981 cttcgccttt gaccgtgatc gtttggttca cagtggcgca aggttcaacc acgtacaccc
    17041 gcaccccgtt caccgtcctg tctggaacag gaggcgtggc tgaacagggt gactgaatat
    17101 cggtaaaccc ttcctttttt tcggttttgg gctgggtaaa atccagctgg aacatagcgc
    17161 tgacgatatc cttcgaacgt tttgagtttt cgaccagcgc gcgtaaatta aactcggtta
    17221 cctgcccgcc aattccgtac gccatgagta gccacaggag tatcgggaga acagcgttct
    17281 ttcccccggg cagcgaagat gcgtcccaaa tgttccacaa ccatataagc gcgggcagaa
    17341 tgtaccaggc gggtttacca taccacgcca caacagcaat cgaagtggca accgccagga
    17401 aaatggcgat cgcagcacga tattttttct ggatgagttg tcccagtcca ggtaccaggg
    17461 cagaaagaaa aatcgagacc aatctggagc gagatgtttt ttctttcatg agtgtcaaac
    17521 aagagcggtg gcgcgcatat cctgcgcttc ttcgccataa atctctttga acttagcgcg
    17581 gttgatttca gatggcaatc cctcaaaaac cttgacccca tctttcaagc cgatcacccg
    17641 ttccgcgtag cgctgaatga gatccagata atggaggctg caaataacac ttacattgtc
    17701 ttcttcattt aacttttcga tatattgcat gatcgaatgc gccagcaccg ggtcaaggct
    17761 ggcaaccggt tcatccgcca ggatcagctt gggttcctgc atgagcgtac gggcaatgcc
    17821 aacgcgctgc tgctgccctc ctgaaagcgc gtcagcccgg ctgtgaactt tatccgcaat
    17881 accgacccgc gtcatcgcgg caagcgcgcg ctccttctct tcatggggga aatatcccag
    17941 tgtggagctc caggagttta gataacccaa cctgccgctt aacacgttgg tcataaccga
    18001 agagcgtttc accaggttga aatgttgaaa gatcatacca atttgccgtc ggatatgacg
    18061 caactcagcc cctttggcgg cggtaatatc aatcccgtcc atggtgatct tcccttctgt
    18121 ggggtcaatc aaccggttga tacagcgcag cagggtggat ttacctgaac cggatagtcc
    18181 aatgatgacc agaaattcgc catccggcac ttcaaaactg aggtctttga gtgccaccgt
    18241 accattggga taaaccttcg tcaaatgttc gactttaagc acggttaatt cctttttaac
    18301 tatttagggg aaaaactgga ggggggaaac ccccctccag cgaaacagaa gaacttattt
    18361 caccatggtg gtgatatcca cacctgaagc ctgcagagcg gagcggaaag cgtcatagaa
    18421 cgaatcttca gcgggtttga gatcctcgat ttcatagcca gcatttttga agatctcttt
    18481 accagcctca tcagcggcca tggcaacaaa gccatcatag actttggcgc cgatctcttc
    18541 tggcatggca gaggagaagg agatattgtc attgggaatg atgttgtcag ttctccagat
    18601 gaccacgatc ttctcgttga catccgtatg aatcttatca ctggtgcggg cgtcaatata
    18661 ggtggcaccg aagtcgcaga tagcgccaac ttttttatca ccagaaacgg tcacgccagc
    18721 gtaaagggcc tgcaccaccg cggggtgaga ttgcacggct gcgggggtct taaaattaac
    18781 cccttgttct ttgagaacac caaagggaat cacataaccg gatactgaca gaggatcggt
    18841 aaaacatggg cgtttgcctt cgaactgggc gagcgcggtg gcggcgtcag cggtatcggt
    18901 attggtgttg gggtcaaagt acggggtgaa gcccatatct ttgtgggcga tgaattgggc
    18961 accgtagaaa tcagagccat tgcgaacaac gaccagcttg acgtccattg cgcccgcttg
    19021 cttgcccagc aggtatgcca aggtgggcat aaagccaata tgggcgttgc cggaagccat
    19081 ggcttcaatg agggcggtgt agctgttggg aataatcacc gaataggtca gtccggtgag
    19141 ttctttcatc ttcgctgcga tcgcctcacc gccaacggtc aactgttcag cggtggcaga
    19201 aggcgccagg gcgatgatga tggggttttc cgcagttccc agttcctttg gcgcttcagt
    19261 cggttggggg gccgggggtt cggtcgctgc tggtgcttcg gtcgccacgg gtgcttttgt
    19321 gggagccgcg gtcggctggg cgcaggcact cagaacaagg gtgctcaaca ccagcactga
    19381 aatgagaaca aacattgctt ttttcacttc tctcctcctt ggattaaacg tacaggtttg
    19441 gcgtctttga taaaaacgca atatcaatca tataccacat atttttaaaa agcaagcaac
    19501 tagccttggg ttcaacctta atattttccc gttcataagc catcgcaata gaaccccgca
    19561 caataccaag agagaaattc ggactgtagt cagttttttt ggtgcaattt tccaggtcct
    19621 gcggcgcaaa cctatattac atttatcctc atttgtccga caaggacaac tttttccttg
    19681 tattcatttt tttttcatgc taatatcaat atggcaaaga tcctcaattg tttggtagta
    19741 aaaaataaac tgcgctcttt tatgcgtcag gtttttgctg ttcaaaatta cggttgccaa
    19801 gaaagcgagg tgaaaaaagc ccgataagaa gaattcagaa atcgattcct tcaaatgtag
    19861 ttctattctc aacagcggtc aaaaaaccgt gcaacacagg gtgagaaaaa gccctcaggc
    19921 ttgaatctaa aaacaaaaag ataggacata ctatgatcaa tcctcatcca ggacttccag
    19981 aactagagta tgtaaaaccc ggtaccctcg ccgaggcgtg taagttctta gagcaacacg
    20041 aagggaaagc ccgtcctttc tccggcggaa cagattgttt cgtccgcctg cgcgatggtg
    20101 tactgaaact agagtatctc gttgacataa aaggtctcga cggtacaaaa gagctttcgt
    20161 ttgaccccaa aaaagggtta acgatcgggg ccgctattac aatgaaccag gttatcgccc
    20221 acaaagacgt ggtgaagcat tatcctaacc tggttgaggc ggcaaaatcg gtggcaagtt
    20281 accagttgcg caaccgcgcc accatcatcg gtaacatctg caacgcctcg cctgccggcg
    20341 ataccatcgg cacctgcctg gtgttggatg gcgtcctcaa cgtgcacggc acaggcggaa
    20401 aacgcactat tccattaaac actttcttcc tggctccagg acgcaccgtt ttgaaaccgg
    20461 gcgaagtggt cacctccatt acccttcctc ctccaccagc cggcatggcg ggtgtttaca
    20521 agaagttggg caggaataaa atgagcgatc tctccatcgt cggtgttacc gtcatggggt
    20581 atcccaacag tcaatcacca tcaggcttct ctttccgtat cgctctggca tcggtcgccc
    20641 cggttcctat ggaagcattg aaagcggaag ccattctcgc tgagaaaaag atcacagccg
    20701 aatcgatcgc gctagctgct gaagcggcga tggaagccgt caccccaatt gatgatgtgc
    20761 gcggctcagc ccgttatcgc aagtacatgg tacgcaacct cacacgcgaa gcgatcacgg
    20821 aagtgtggaa cacattaacc aaatagctcc ggctgtttga cctttacagg aggaatgaat
    20881 gtcttattca aaaatcacac tcaccgttaa tgatgtcgtc gaaacggttg atgtccccca
    20941 tcacatgacc ctgctgcaaa tgctgcgcga aaaactctcc ctcaccggta ccaagaacgg
    21001 ttgttcggct ggtgagtgcg gagcctgcac tgttctgatg aatgatgaac ctgtcaatag
    21061 ttgtatggtt ttggctgcag aatgcgatgg tgcgcgcatc gtcactgttg aagggctggc
    21121 aaaaagtggt aaattaagca agttacagga agccatcatt aagaccggcg gcgtgcagtg
    21181 cggtttttgc acccccggca tgcttatttc agccactgct ttgctcaaac gcaacccaaa
    21241 cccaaccgaa aaagaaatca gggaagcgct ggttggcaac ctgtgccgat gcacgggtta
    21301 tttcagaatt gtcgaagcgg tcaaagaagc tgcgcattca tagaccttaa gacgcaataa
    21361 ccaactttag acaggagaaa tcctttaaag gagccgaaat atggcaaacg cgaaatcaac
    21421 tgaaaaaacc acggcggtcg tcggtacgag tgttatccgt aatgatgtgt tggaaaaagt
    21481 gaccggcgcg gccacttacg tcgacgacat ccagtttggt aataaattgc tatacgcccg
    21541 cgctgtacgc agcccacatc cgcacgcgct gatcaagcat attgatacca gcaaagcgga
    21601 aaaactccct ggtgtcaaag ccgttgtgac tggtaaagac ttccctgaac gcatcggtct
    21661 ttaccttaaa gataagacca tttttgccac cgaccgtgtg cgcttcttgg gagaaccggt
    21721 agcggctgtg gctgccgtta gcgaagaaat cgccgaaaaa gcctgcagcc tggtggaagt
    21781 aaaatacgaa ccgctcgagg gtgtttttga cgctgaatat ggcgcttcag aaaaagcccc
    21841 cgtcattcac cctgaccttg gcaaatatga ttgtgtcaat ttcatcttcc ccaagcccgg
    21901 gaccaacatt tccaaccact ttaaaattcg caagggcgat gtggaagccg cctggaagca
    21961 gtgcgcgcac atagtggaaa agaccttcaa agtcccacat attcaacacg tgcctattga
    22021 aacgcatggc gcggtagcca tgatggatga aaaagggaaa gtcactgtgt gggcggcgtc
    22081 acaatcaccg ttcgcccaac gcaatctcat cgccaaagcc ctgcacattt cacacagcga
    22141 cctgcgcgtt atcgcgccgc ttgtaggcgg agggtttggc tccaaagccg gtctcaccat
    22201 ggaggccatc cccgtagccc ttgccaccaa ggtcaaaggc taccccgtga aatttatact
    22261 cacccgcgaa gaggaattct atactaactt cgtgcgccag ggtctcacca tccacgtgaa
    22321 agtcggctgc gataaagatg gcaatatcct cggtctcaag aatgtgatgt actgggatgg
    22381 cggcgcctcg actgagtacg gcacgaacat tacccgcgct ggtggttatt ccagttccgg
    22441 tccttatgat atccccaatg tgtggacaga cagtatctgc gtttacacca accaccccat
    22501 cggcggcgcc taccgtggtt ttggtatgtc tgagctgcac accgggatcg accaggcgat
    22561 tgacgaactg gcagaagtag ccggttttga ccgtgtcgag ttcctcaaaa agaacgccat
    22621 ctcagaaggc gacattgtgg tcactggtat gaccatgcat gccactggca tccagcaatg
    22681 catcgataat gtagccaaag ccatcgaatt ttcaaagaaa gaagaacctt cttctcctga
    22741 ccgtttgcgt ggtaagggca tcgccatcat gtggaaagcc cccgccatgc cccccaacgc
    22801 cggctcgagc gcctgggtgg aactctctga agacggtaaa gcgaccgtcg gacttggcgg
    22861 gcaagaaatt gggcagggta ctttcaccgt gatggcccag attgctgctg aaaccctggg
    22921 cgtcaaatat gaagacgtgc gcatcgccgg accggtagac acgcaataca gcccttacga
    22981 atggcaaacc gtcgccagcc gcctcacctg gagcatgggg aacgcagtac gcctggcggc
    23041 gctcgacgcc cgtaaacaga ttttggacgt ggtggctgaa gcctggaatg aaacaccaga
    23101 agacctggat atcgtcaacg gcatggtcgt ctcttacaag agcgaacagg aaaccccgtt
    23161 ggccaacctg gtcatctatg gtcttcccaa gcccaatgat gaaggctgga tgggcggccc
    23221 gattgttggt cgcggttcct tcatgcccaa gtacgtcacc aacctggacg cggagaccgg
    23281 tcagggcgcg cgcgctgtgg tacattacac cgtgggctgt gaagggctcg acatcgaaat
    23341 tgaaaagaac accggccgca tccacgtgat caaagctgcc gcttcctttg acgtgggcaa
    23401 ggccatcaac ccggcgctgg tcaaagcaca ggttgaaggc ggctttgtgc aggggctcag
    23461 ctccgccatg ttcgaagaaa tccgcctgcg caacggcatc atgaccaacc ccagctttgt
    23521 ggattaccgt atcgccacgg cggccgacgc cgacttcaaa ctggatactc agtatgtcga
    23581 ggtgccgcag gatgacggac cctggggcgc gcgcggcgtg ggagagcacc ccatggtgcc
    23641 caccatcgca gcactgggta acgccatcta tgacgccact ggcgtccgcc tgcaaggccc
    23701 gccgtactca gccgaaagca tctacctggg tatgctcaac gccggcgttg tcaagtagaa
    23761 taacctttga atgccggatg ttctcatccg gcatttcact gaaaaccaca aggagaatta
    23821 tgactgacca ttacgacctg atcatccgta acgcgacact gcgcaacgag ccaaaaagca
    23881 cccaagatat cgccattcaa gatgggaagg ttgcgctcat cgccaacaag atagaagcca
    23941 aagccgccag cgaactggat gcccgcggta acctggtgac agaatcctat gtcaataccc
    24001 atctgcacat gtgcaaggtg tatacgctga tgatgatgga cgaagccgcc ttgaaagact
    24061 atcacggcgc cgacatgggc aaagccatga ccgccatcga actggctgcg cgtgtgaaag
    24121 aaaaatatga cgaaaaatgg attatcaaga acgtcagaaa ggccgctgcc ctggctgccc
    24181 gttacggctg cacccacatc cgcgctttcg ccgatgtaga ttcaaagggt aagctcgaag
    24241 gaataaaagc gcttatcaaa gcgcgcgacg aattcaaggg catcgtggat attcagactg
    24301 tcgcttttgc ccaggatgga ttggttcgcg agcccggtgc tgacaaactg atgcgccagg
    24361 caatggatct tggcgctgat gtggttggcg gcattccctg gatcgaatac accgatgctg
    24421 atatcgccga gcatgtgcgc atctgctatc aactggcaaa agagtatgat aaacccgtct
    24481 ccatgctggt ggatgatgct ggtgataacg ggttgcgctc gcttgaggtg atggcgctgg
    24541 aaaccatccg tactggctgg cagggacgcg cgctggcaca ccacgcccgc gcgatggcgc
    24601 tctaccccaa gccgtacctg caaaaactcg ccgcaatcct caagaaagcg gatatgggcg
    24661 tggtgagcga tccgcatacc gggccgctac acgcgcgtgt acgtgaactg ctggaagaag
    24721 gtgtgctcgt ttgccttggg caagatgata tctccgacgc ctactaccct tatgggcgca
    24781 ataacatgct tgaggtcgcc ttcctcaatt ctcacttgct ctggttcacc acccgcgaag
    24841 acatggaagt gctctatgat atgatcacca ccaaaccggc tcgcgcgatt ggtctcaaag
    24901 atttcgagtt aaagaagggt gcccccgcca acctggtggt ccttgaccag cccaacgtgc
    24961 ttgaagcgct gcgctaccat gaaaaaccg
//
LOCUS       DAOSPM010000018        24927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2008_length_24927_cov_17.515519, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000018 DAOSPM010000000
VERSION     DAOSPM010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24927
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2008_length_24927_cov_17.515519"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLW25_02970"
     CDS             <1..769
                     /locus_tag="PLW25_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53482.1"
                     /translation="SQTAAVPPTAEPTATVAIPTAAPEVLSPPNTPEPAFSPTRGGVW
                     LTVQENTNCRSGPGSLFDFMGLINAGQQVEAVARSTVGEYYYIKNPTSYSDYCWLWSR
                     YTTIAGDTGTLPVFTPQPSPTPKVTPTFTKAPADFFVKYLDTQTCSGLYGIQLYIENT
                     GSTTWQSIRVRLDDNTSAVTTNHKNNYFFGTDGCVKDLSNRQGDLTPGEYSRVALVDD
                     GELSYNPSGHSFTAQVTLYSGDDQTGVSVTKTITFTP"
     gene            802..>1679
                     /locus_tag="PLW25_02975"
     CDS             802..>1679
                     /locus_tag="PLW25_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOS53483.1"
                     /translation="MMIKRISLTVALLFIAAFTLSACQGGTTPVAPIEQSDLQTLVAG
                     TLTAAAVEMQQTMTAAVTPTETIAPTLTETATLPPTDTPTKEVVSITLSGNTNCRRGA
                     STYFPVITVIPAGSVVEVIARNPESDYFFVRAAESDTGGCWIWKEFASVTGNLESLAV
                     YTPVPTPLPTATNTPPPGANFTAQFVGLTACGTGYAANFNISNTGTSTLQSIRIKITS
                     TSWNFTFIDNTGFSQWSHGSKYATLAEITSGKSMIVSTCPGGFDSSPSGQDIKAEITV
                     CLNDNLTGACSTQSLE"
     assembly_gap    1680..1779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1780..3462)
                     /locus_tag="PLW25_02980"
     CDS             complement(<1780..3462)
                     /locus_tag="PLW25_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53484.1"
                     /translation="MTWALVKRVFAYARPYRLPLLVILLLISASSGLNLLNPLILREM
                     IDRTIPQGNVRRLVLLAVTLLLVALLNGAISVVNRRLNASVGEGVIRDLRVSLYAHMQ
                     RMSLRFFTNNKVGELMSRLNNDVVGAQNAVTNSVISIVTSLVQSIAVLVVMFTLDWRL
                     TLISMLVAPFFAVVAALLSRRLRSIVRKQLEANASMNAMMNETLNIGGTLLVKIFGRQ
                     AQEVERFSRRANQVKEYGINRAQTGSIFGAIIGLLVAVGTALVYGLGGYFVISGVFTV
                     GTIVAFSSFMLSLYGSVQSLVNAPVDFASSFVSFERVFEVLDQPVEISEKPDAIALTE
                     VEGEIAFENITFAYQAGDGVILSDVHRYGNMDSIVTTLSDGESELEPEERRSQARGNA
                     LENVSFHVKPGQLVALVGPSGAGKTTLTYLIPRLYDPSSGRITLDGTDLRDVTLESLT
                     RQVGMVTQETYLFHDTIRTNLLYSRPDASQAELEEACMAANIHDFVRELPDGYDTVVG
                     ERGYRLSGGEKQRLSIARVILKDPRILVLDEATSSLDSEAEALIQEALKRVMR"
     gene            3831..4466
                     /locus_tag="PLW25_02985"
     CDS             3831..4466
                     /locus_tag="PLW25_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOS53485.1"
                     /translation="MQSKPKLAIFDFDGTLADTRPFMLSILDHLAERFNTRRVDIHSL
                     EQMRGYTARQLMELYRVPMWKFILMARESQRLLYQNITSIQLFPGVESALCAIARRNV
                     AIAVVTSNQLRNVLAVLGEELAGLVSVFECEAGFFTKARKLRSALRQAGVKPGEAVSF
                     GDESRDIEAARKVGIPCAAVTWGYAHSEVLRHHQPDYLLTSVDQIMGIVAE"
     gene            4635..>4816
                     /locus_tag="PLW25_02990"
     CDS             4635..>4816
                     /locus_tag="PLW25_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOS53486.1"
                     /translation="MGYIMEALHYLLSGLGAFAAGAVNALAGGGTLITFPLLTALGLP
                     AVSANITSTVSLFPGY"
     assembly_gap    4817..4916
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4917..5289
                     /locus_tag="PLW25_02995"
     CDS             <4917..5289
                     /locus_tag="PLW25_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOS53487.1"
                     /translation="SPRLWLISLPVCLAAIYGGYFGAGLSVILLAILALFLDDSLTRL
                     NGLKQFLAFAANSGAAIFFLFSGRVNWRVAGVMAISALLGGIAGGKLAGRVSPVVLRW
                     VVVSIGVVVAVIFFIQIYLKP"
     gene            5304..6491
                     /locus_tag="PLW25_03000"
     CDS             5304..6491
                     /locus_tag="PLW25_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS53488.1"
                     /translation="MLNTKPRLALIIACSMYLAFGMFNAAIGPVLGELAENTHSTLVM
                     VGGVITFLFLGSLATQLIAGPIIDKFGLRIVGLVSLLLVAFGLPAFTNVHSYPLMLAL
                     VLLTGLGQGGMDLTANLLVADAYPNKSTPMLNLLHFFFGLGAFFGPALVGLAIAATGS
                     GMIIHWVAAGMFLLLALILLRVPAGKAPAPDAAKKPDAKVKDEKSIYLSPFLWTIGVL
                     ILIYVGTEYGLGSWSTRYMGLTTGMADQNGALVTSAYWGALTLGRLAGAAASRRLSNT
                     RLLGVSLGGSLLGTLGLVLSRGSVLSTVVFIVITGFSLGTVYPTSVAVTVEKFPKDQG
                     KTVGLLAAMGSIGGLALPWLAGFLLETTSALVFALFLCATTVAMLLLLFVLGQYKKGT
                     SNG"
     gene            complement(6562..7896)
                     /locus_tag="PLW25_03005"
     CDS             complement(6562..7896)
                     /locus_tag="PLW25_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOS53489.1"
                     /translation="MKTRWFLQVSLILSILVSFFQPLQLVSGQVDAPEAVVVQLKGPL
                     TPVWSGYLQRGVNRAVSTGAGLLIIELNTPGGSVDLMNHLVQQILASPVPVVVYVTPR
                     GAMAASAGTLLVLSGHFAAMAPESTIGAASPVGMQGEDIGSTLEEKTKEILKASARSL
                     AARRGADAVKLAEESIEKARAANSTEALQAGLIDFIARDVEDLLAQMDGSKVLMNGNE
                     VVIRSKGALQVPVSTTMVEDVLGLLTNPNIVFLLLSIGVQAILIEISSPGGWVAGFIG
                     AVLLALAIYGLGILPVNWFGMIFLVIAFVLFILDIKAPTHGALTVAGTGSFIAGALIL
                     FNSLSIPGFPRVSVGLVIGMGVFLGLSFFAVVMIALRAMKKPVITGRESLEGKDGYVV
                     TRLAPIGIVQVAGEQWSAVLAPGEPPLKKGEKVIVEKVEGVRLVVRHIETKT"
     gene            8044..9936
                     /gene="dnaK"
                     /locus_tag="PLW25_03010"
     CDS             8044..9936
                     /gene="dnaK"
                     /locus_tag="PLW25_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOS53490.1"
                     /translation="MGKIIGIDLGTTNSVVAVMQGGDAQVINTAEGARLCPSVVAFNK
                     NGERLVGQTAKRQAVINPENTVFSIKRFMGRRFDEVESERKMVPFEVISGPMKDARVR
                     IPVTGRDYSPQEISAMILSKLKADAEAYLGEKVSQAVITVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTASALAYGLDKKKNEKILVFDLGGGTFDVSVLEVGEGVFEVKSTSG
                     DTHLGGDDWDEVIVNWAADQFQADQGIDLRKDRQALQRLREAAEKAKIELSTVMETEI
                     NLPYITADANGPKHLVTKLSRSRFEQMTAHLLDRCKKPFESALKDAGLKASDINEMVL
                     VGGSTRMPQVVDMVRGLINKEPNKGVNPDEVVAIGAAIQGGVLGGEVKDILLLDVTPL
                     SLGVETMGAVMTPIIERNTTIPIRKSQIFSTAEDGQTAVDIHVLQGERPMAADNMTLG
                     RFRLEGIPTAPRGIPQIEVTFDIDANGILNVTAQDKATGREQKVTITASTNLQTSDIE
                     RMVEEAKAHKSEDDRRKELAEARNIADNAAYQVEKAVKEAGEKVTASDRSSIEARVQE
                     LRSAMTGEDVSRIKSLTETLQQDYARVMQASAQAQTAPGGGDAGGSAPPDDGGDVVDG
                     EFTEKN"
     assembly_gap    9944..10043
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10221..11579
                     /locus_tag="PLW25_03015"
     CDS             10221..11579
                     /locus_tag="PLW25_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HOS53491.1"
                     /translation="MLEFSHSYYSQANMSASVPSTFTPTPIVRVAERLLTAFLKVAAK
                     QYLSLQPTNLSSVPPPQPGHRYTLYAHVPFCESLCPYCSFNRFVLDREKAAAYFKSLR
                     AEMRLVANLGYRFSSLYLGGGTPTIVPDELARTIDLARELFSINEVSCETNPNHLNPE
                     MVTLLKERVQRLSVGVQSFNDGLLKQMNRYQKFGSGASILESIRRTAPHFKSLNVDMI
                     FNFPSQTPQILRDDLHAVIDSGAQQVTFYPLMSSPSVRRSMASTVGELIPAREADYYA
                     IISQGLAQDFSALSAWTFARNGSNLIDEYIVDNEEYVGVGSGVFSYLNGCLYASTFSL
                     QEYSDLVSQGRMALSGAQPFSRRQQMRYRMMMDMFGLRFDRAAFRQRFGVPASRGLWL
                     EMLFFRLLGAFEKKNPAIPTPVGRYLSLVIMREFFTGVNQVRDIARAALSPYDRGECY
                     AP"
     gene            11980..13320
                     /locus_tag="PLW25_03020"
     CDS             11980..13320
                     /locus_tag="PLW25_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOS53492.1"
                     /translation="MRYRRKWLLLILVVLIVAGLTLSLPPVWSRITYHTHNLFADIKY
                     RLNPPQAEVFVPSGAEEGNLIATASAATLTALVPTPTSPILTTEETRLPSSTPTLTPV
                     PLPIKVYLPGVKPEPQLWNNCGPATLSMNLSFYQWGKTQVEAAEVLKPNPRDKNVMPY
                     EMVTFINTQTSLRALSRMGGDVETLKRLLNAGFPVLVEKGFEPGDLKKEGWMGHFNLV
                     IGYNDDAQSFITQDSYLLSHPPGGGEIKDLETFPGFTVPYEDMQKDWRAFNYVLIVVY
                     PPEKENDVLNALGPLMDENGAYRLAYERAKMEATTLSDIRERFFAWFNAGTSLVYLAD
                     YNGAAAAYDSAFNLYAQIPENARPWRMMWYQTGPYFAYYYSARYTDVINLADQTLKRM
                     SAEPILEESYYWRGMAYLALGDNERARAEFRDSLKYHPGFGPSLVALEQMGETP"
     gene            13357..14595
                     /locus_tag="PLW25_03025"
     CDS             13357..14595
                     /locus_tag="PLW25_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOS53493.1"
                     /translation="MIKILHFADAHIDIATHGKHDPETGLPIRALDFLKALDTIIDHA
                     ISEKVDLVIFAGDAYKDRTPSPTFQREWGKRIIRLPQADIPALLLVGNHDISPASGRA
                     HTLQEFDTLQVPKVRVLYKPELLKPADLWGLQLQVLSLPWVFRSSLVAALGLSAVEED
                     TVHEEIEKRLTTILQARMDELDPALPTVLVGHGSVQGAVYGNERTVMLGKDLVLPGSL
                     VKDPRLDYTALGHIHKYQDLNPGLHPPVVYAGSIERVDFGEVADDKGFVIAEVEKGNT
                     SYRFVKLHGRAFFDRLVRLSMEDRAMEKILASSPNEAEAENAIFRLVIEYPRELEADI
                     DEPALRQKFASALEFHLVRRPQAEARLRLPADQNIASYSPLELLSTYWKSTHAQPRDL
                     AGLQTLAQSIFQSVTGSKPG"
     gene            14616..15944
                     /locus_tag="PLW25_03030"
     CDS             14616..15944
                     /locus_tag="PLW25_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOS53494.1"
                     /translation="MSNTLIEILVIFVLILFNGFFSMSEIAIVSARKVRLEQRADEGD
                     AGAGRALQLSKETGQFLSSVQVGITLVAMLTGAFGGATLAERLTQSLARVPWLATSAA
                     TLSLVIVVLVTTYFSLVIGELIPKKIALNNPEKIASKVSGIMVFFSRLTSPLTRLLSS
                     STDLGMRLLGIKPSGEPPITEEEIKVLIDQGTQVGVFGEAEQDMVEGIFRLNDRSINA
                     IMTPRTDVTWLDADDSIEEILKTILQSPYSRFPVSRESLDNVLGILKSRDLLEKQLSG
                     EPFNLLDLLQKPLFIPENTPVMRVLEMEREHGVHEALVIDEYGGVLGFVTLFDVLEAI
                     IGELPSQDQASEPEIVMREDGSYLIDGLLPIDEFKDLFDLDLLPEEDKVGFQTAGGFV
                     MNQVGSIPHAGQTFQWNDLLFEVLDMDGLRIDKILVSRNSEKTPPAGGDK"
     gene            15941..18505
                     /locus_tag="PLW25_03035"
     CDS             15941..18505
                     /locus_tag="PLW25_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOS53495.1"
                     /translation="MIIDRLQISGFLSYKEKVDIDFSGFALACISGQNGAGKSSLLEA
                     ITWALFGEARRKDDAIIHMGTDTAEVLLEFDYESTRYRVQRSKTRDKSTQLEFQLRNA
                     EGNWVPLTEATMRGTEELIRRTLHLDYETFTNASFFLQGKADQFAQQRPGDRKRILSG
                     ILGLDIWEDYREEAARRRKLLENDLALVISHLQEIETELQQEAERKERLARLEKEVST
                     QTELFEARKAYLEQQRQVAERILADQKQVEKQRAEINRLRAEDETMRSDLDLRTQERQ
                     THLSLLQQEKEIRQEVDAWEQARKTLEEWDTLAQDFHQYDSQRQAPLQAIAVEESRLQ
                     AELNSLQQKQAEIFSIEQELPEIQRLVEDSSKAVEDCSAKLATRAQLEKEMRALLDEI
                     ARARAENTRLKMEMDEIVEHRTHLENISGANCPTCEKPLSDDERQRLVDEINARGKEK
                     GDAHRENKRIIEQSEAQYREIETALLNLQRVDAELKLQQRIFDTKAQELRMKEAAFTD
                     WQQKGAVRMLELEETLRSQSFALEARKQLAMMDAQLKKLGYDAAAHEQARRMELDGRA
                     SQDKKIQLEKARSALAPLEQNITSLQKNMENNQKRLSALLDELRQDEEKLEIETSNLP
                     DLDQLERDYYAAQEQMNQANRDLGYTRNQVEVLEKQRELKVEKLEEKDQLNNQVTDLK
                     TLERAFGKDGIPALLIEQALPEIERHANEILDRLSSGSMSISFETQRDFKDKKREERR
                     ETLDILIRDPAGERPYELFSGGEAFRVNFAIRLALSRVLAHRAGARLQTLVIDEGFGS
                     QDADGRQRLVEAINLVSTEFEKILVITHLEELKDAFPARIEVTKTPAGSKVEVIAV"
     gene            18502..19602
                     /locus_tag="PLW25_03040"
     CDS             18502..19602
                     /locus_tag="PLW25_03040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS53496.1"
                     /translation="MNMIHARVGLQQRVLPEYRAEFFDTLATACLQGLGVFAGEPCQG
                     EAVEVVDQLSIAYYTHARNLHFFRGSAYFYWQAGVLGWLQRWQPDVLIMEINPRNRSN
                     PLAARWMKERGRPVLGWGLGLPGAGKIYRLIYHSLLRSCDAVIAYSTTAALQYRHAGV
                     DPSRVFVAANAVTRRPTDLVVARPPSFHENTPQLLFVGRLQPRKRVDTLLYALAMLPP
                     HQQPRLSIIGDGPDRSRLEMLAWQVYPRAVFHGAKRGQELDPYYQAADLFILPGTGGL
                     AIQQAMAHALPVIVGAADGTQIELVREENGWTLPDDSPETLARVIREALSDVERLRQK
                     GLASRRIVSEEVNLEGMVKTFCTAVESVLSKA"
     gene            19614..20792
                     /locus_tag="PLW25_03045"
     CDS             19614..20792
                     /locus_tag="PLW25_03045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS53497.1"
                     /translation="MNLLIIADGRSPITRRWIRMLQPLNYTINLVSTYPCQPVDGVNE
                     TILFPVAFAGLSGSQAGGGARGGRKVVSRYRGLAATLRHWLGPLTITHAEKDYLELLN
                     RLQPDLVHALRIPYEGMLASRTPTGIPLVISTWGNDFTYHAISNGVMGSATRRALLRA
                     DGLISDTVRDLRIARAWSFNPAKPAVSVPGNGGIDLAEIENITQGIDPAVPWQVINPR
                     GFRSGSVRNDTFFKSIPLILSLYPEVQFICPWMAGQPEALSWVNRLRILDNVSLLPMI
                     SQAELWREFARSLVSVSVSEHDGTPNSLLEAIAIGCLPVCGDIESIREWIRNGENGIL
                     VDPADAYALAEAVQRGIMDEEFRRAAAARNREIIRERAEITIVRQKVAEFYQRMTGFV
                     "
     gene            complement(20834..21940)
                     /locus_tag="PLW25_03050"
     CDS             complement(20834..21940)
                     /locus_tag="PLW25_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS53498.1"
                     /translation="MNSKVAVLKTTPETVIEDYIRLFELADGPKALDPGATTILKDNI
                     SWHFPMPSANTTPWQMEGTILALKSKGYNDITCVQNQTVVTNAFKGEDLNGYVPIFKH
                     YHIPVLYNFEDKDMTWVKYKPKAKMNALDHIYPEGIYIPDYFVGKNIVHLPTAKCHIY
                     TTTTGAIKNAFGGLLTKHRHYTHSYIHETIIDLLAIQKEIHTGLFAVMDGTTAGNGTG
                     PRVMKPEVKNVILASQDQVAIDAVAAKLMGFDPMSIKYINLGHNQGLGVGDPREIELV
                     GDDISGENWHFKVGHNLHQFMGWLTWYGPTKFLQKLVTRTPLVAIPIAFSEVYHDYVH
                     WPLKEKYVFKEWCQNTGWGQLFMRYKKEGVIHNL"
     gene            21968..23605
                     /locus_tag="PLW25_03055"
     CDS             21968..23605
                     /locus_tag="PLW25_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOS53499.1"
                     /translation="MGELYQQAVRLANPACVRATPADLVYNPSEFMNKKMPLKQPGWN
                     FRSRACGLLICGLLLLASCNFPIRDFSALPRPYATLDPAIFEYHRSATPTAGQPGAAP
                     TRTPQPPPVVDESLYHIYTIRSGDTFETVARHFSVTPAAIISMEALPQTGILPPGRYL
                     VIPKNTDNLFEQEMILPDSAVINSPCAAPFDISAYVSAAGGYLSTFSQPVAGIRSSGA
                     EIVRRVADNQSVNPMLLLAILEHRAGLVFGKQPPADINHPLGFRNMYYKGLYQELSLA
                     ARMVNTGYYGWRYGTMTMVTFTDQVSQRISPHLNAGSVALMYLFSQSYPSTDWNERLL
                     GKSGFMETYLAMFPDPWLCAAEVEPLLSDTLSAPQLELPFAPGEEWAFTAGPHYSWVE
                     GTPLGALDFAPNIKSAGCRVSPSWVRASAPGVITRADNGVVILTLEDEKGDVTGWEVM
                     YLHIAANERVPLGTRLRKDDPIGHPSCEGGSSTGTHVHIARKYKGEWIGAQGLFPFVL
                     SGWVAYPGERAYQGRLMKDGQVVIARQGGNQDSLIKR"
     gene            complement(23699..24190)
                     /locus_tag="PLW25_03060"
     CDS             complement(23699..24190)
                     /locus_tag="PLW25_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOS53500.1"
                     /translation="MNAKVYEAMNDQIKHELYSAYFYLSMSAYCETENLPGFAKWLKV
                     QAEEEQEHAMKLYDYLLDRGEKISLKAIEQPPVEFKGVKDVFTKVYEHEQKVTGLING
                     IYAKAVEANDVASQIFLQWFINEQVEEEKNASSVLAMVTKLEGSLGGLYQLDHQLGMR
                     EAD"
     gene            24319..24504
                     /locus_tag="PLW25_03065"
     CDS             24319..24504
                     /locus_tag="PLW25_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53501.1"
                     /translation="MCAFQDRLNAIALGAVLAENLCMLLEFLSTRALQDLETEMIDKT
                     LPAGASPRLGVAVCQSA"
BASE COUNT         5565 a         7002 c         6501 g         5559 t
ORIGIN      
        1 cagccagacc gccgcggtgc ccccgacggc tgaacccaca gccaccgttg ccatccctac
       61 tgccgcgcca gaagtgctat ccccacccaa cacacctgaa ccagccttca gccccacgcg
      121 aggaggggtt tggctgaccg tacaggaaaa taccaactgc cgttctggcc caggcagcct
      181 gtttgacttt atggggttga tcaacgccgg tcagcaggtt gaagcggttg ctcgcagcac
      241 ggtgggcgag tattactaca ttaaaaaccc tactagctac agcgattatt gctggctgtg
      301 gagccgttat accaccatcg cgggcgacac aggtaccctg ccggtgttca ctccccaacc
      361 cagccccacc cctaaggtca ctcccacctt taccaaagcg ccagccgatt tctttgttaa
      421 atacctggac actcagactt gttctggttt gtacgggatt cagctctaca ttgaaaacac
      481 cggcagcacc acatggcaat ctatcagagt gagactggat gacaatacca gcgcggtgac
      541 cacaaaccat aagaataatt acttcttcgg tactgatggg tgcgtgaaag acctgtccaa
      601 tcggcaaggg gatctgaccc caggcgagta ttcccgtgtc gcccttgtcg atgacggtga
      661 attatcctat aatccttcag gtcacagttt caccgctcag gtgacccttt atagcggtga
      721 cgaccaaacg ggagtatcgg taaccaagac catcactttt accccataaa cgaaagtatt
      781 tctgtactgg aaaaggaacc catgatgatc aaacgcattt ctcttacagt tgcattgctt
      841 ttcattgccg cgttcaccct cagcgcctgc cagggtggaa ccacgccagt ggcgccgatt
      901 gagcagagcg accttcagac cctcgtggcc ggtacattga ccgccgcggc agtggaaatg
      961 cagcaaacca tgaccgccgc ggtaactcca accgaaacga tcgcgccaac cctgacggaa
     1021 accgccactc tgccgcccac cgataccccc accaaggagg tcgtgtccat caccctcagc
     1081 ggcaacacga actgccgcag aggggcttcc acttacttcc ctgtcattac cgttatccct
     1141 gccgggtctg tggtagaagt gatcgcgcgc aacccggaga gcgattactt ctttgtgcgc
     1201 gcggctgaat cagatacggg aggctgctgg atctggaagg aattcgcttc ggtcactggc
     1261 aacctggaat cgctggccgt ctacaccccg gtgccgacac cattgcctac cgccaccaac
     1321 acgccgccgc ctggagcgaa tttcactgcc cagtttgtgg ggctcaccgc ttgcggtacg
     1381 ggttacgctg ctaattttaa tatcagcaac accggcacga gcacgttgca atctattcgc
     1441 ataaaaatca cctctacgag ttggaatttc accttcatag ataataccgg tttctcccag
     1501 tggagccacg gatccaagta cgccacgctg gccgagataa ccagcggaaa atcgatgatc
     1561 gtatcaacct gtcccggagg atttgattcc agcccctccg gccaggacat caaagctgaa
     1621 atcaccgtgt gcttaaacga taacctgacg ggcgcctgct ccacgcagag cctcgagttn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cgcatgacgc gcttcaacgc
     1801 ctcttgaatg agcgcttccg cttcgctgtc caggctgctg gtggcttcgt ccagaaccag
     1861 gatacgcggg tctttaagga tgacccgcgc gatggacagg cgttgttttt cgccgccgct
     1921 gagccggtat ccgcgctctc ccaccacggt atcgtaccca tcaggcagtt cgcgcacaaa
     1981 atcatgaata ttggccgcca tacaggcttc ttccagttca gcctgcgagg catccggcct
     2041 agagtaaagc aggttggtgc ggatggtgtc atgaaagaga taggtttcct gggtcaccat
     2101 gccaacctgg cgggtgagcg attccagggt tacatcgcgc aggtcggtgc catccagggt
     2161 gatgcgcccg ctggaggggt catacagccg ggggatcagg taggtgagcg tggttttccc
     2221 cgcgccgctt gggcccacca gcgccaccaa ctgcccgggt ttcacgtgaa agctgacgtt
     2281 ttcaagagcg ttacccctgg cttgagaacg ccgttcttca ggctcaagtt cagactctcc
     2341 gtcactcagg gtggtcacga tgctgtccat gttgccatag cggtgcacat cgctcaggat
     2401 gacgccatcg cccgcctggt aggcgaaggt gatgttttca aaggcgatct ctccctccac
     2461 ttctgtgagc gctatggcat cgggcttttc gctgatttca acgggctgat cgagcacctc
     2521 aaacacccgt tcaaaactga cgaaggaaga agcaaaatcc accggcgcgt taaccagaga
     2581 ctgcaccgaa ccgtacaggc tcagcatgaa agagctgaac gccacaattg tgccgacggt
     2641 gaaaaccccg ctgataacga aataaccgcc taatccatac accagcgccg tgcccaccgc
     2701 caccagcaga ccgatgatcg cgccaaagat cgatcccgtt tgggcgcgat tgatgccgta
     2761 ttcttttacc tggttggcac ggcggctaaa gcgctctacc tcttgcgcct gcctgccaaa
     2821 gatcttcacc agcagcgtcc ctccaatatt gagggtctcg ttcatcatcg cgttcatcga
     2881 agcgttggct tccaactgtt tgcgcacgat gctccgcaac cgtctgctca aaagtgcggc
     2941 aacaaccgca aagaaaggcg ctaccagcat gctgatcaag gtcaggcgcc aatccagcgt
     3001 gaacatcacc accagcaccg cgatggattg caccaggctg gtcacaattg aaatcacact
     3061 gttggtgacc gcgttctgcg cgccaaccac atcgttgttc agccggctca tcaactcacc
     3121 aaccttgttg ttggtaaaga agcgcaacga catgcgctgc atgtgagcgt agagcgagac
     3181 ccgtaaatca cggataacgc cctcgccgac cgaggcgttt aaacggcggt ttaccacgct
     3241 gatcgcgccg ttgagcaaag caaccagcag cagcgttacc gccagtaaga caagccgcct
     3301 gacgttgcct tgcgggatag tcctatcgat catttctcgt aagattaacg ggtttagcag
     3361 gttaagcccc gagctcgcgc tgattaacaa caggatgact aaaagcggga gtcgatacgg
     3421 gcgggcgtag gcaaacaccc gtttgaccaa agcccaggtc atattaggta gtttttcttc
     3481 agtggccgcg agccttctaa taccatgcat catttcgttt accctgctgt tgtatacgtc
     3541 aatgttaggt taaataaatg gattggtcaa gtttttcgcc catctctttc gcccgtttac
     3601 tggggagcga tggtggcagt gcacgtggtt caaccgcagc ggcaacccaa ctggcgccgg
     3661 cggtaccaca agttacccgt gcgatagtca cccaacatgc tggatacatc acgccgcgca
     3721 tgcttgatgg tcaaatgggt gcttggcatg gaagaaagtg ccacgaatga taagtgaatg
     3781 ataggttatc aaatggaaaa agagcaaacc atcggtataa tggaaacatt atgcagagca
     3841 aacccaaact ggcgattttc gattttgacg ggacgctcgc tgatacacgg ccgtttatgt
     3901 tgagcatcct cgatcacctg gctgaacgct ttaacaccag gcgagtggat atccactcgc
     3961 ttgaacagat gcggggttat actgccaggc agttaatgga attgtatcgc gttcccatgt
     4021 ggaaatttat tttgatggcc cgtgagtctc aaagattgct gtatcagaac atcacctcca
     4081 tccagctttt tccgggggta gaaagcgcac tgtgcgccat tgcccggcga aatgttgcca
     4141 ttgcggtggt cacctcaaac cagctgcgta acgtccttgc ggtgcttggc gaagaactcg
     4201 ccgggttggt ttcagtgttt gaatgtgagg caggcttctt caccaaggcg cgcaaactgc
     4261 gcagcgcgct gcgtcaagca ggcgtgaaac ctggcgaagc ggtcagtttt ggagacgagt
     4321 cccgggatat cgaagcggcg cgcaaggttg gtatcccctg cgccgcggtt acctggggct
     4381 atgcccatag cgaagtttta cgccatcatc aacctgatta cctgttgacc agtgtcgatc
     4441 agatcatggg cattgtggct gaataataac aggcaggtaa aagagcgtgc gcattgcttt
     4501 aagaacctga acgattgaga attttgccgc gtttcttcac aaaaaggtca cgcgttcctt
     4561 aatgtcgctc ttgctttgtg agccaaattg ggtttttgcg cgcgtattca cggcgggagc
     4621 aaaaatcaac tacaatgggg tacatcatgg aagcccttca ttacctcctt tccgggctcg
     4681 gtgctttcgc tgcaggcgcg gtcaacgctc ttgctggcgg cggcacgctg atcacctttc
     4741 ctttattaac cgccttgggg ttgccagcgg tgagcgctaa tatcaccagc acggtatccc
     4801 ttttcccagg gtacttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctct
     4921 ccacgcctgt ggctcatttc actgccggtc tgcctggcag cgatctatgg cggttatttt
     4981 ggcgctggtt tgagcgtgat tctcctggcc attctggcgc tttttttgga tgactctctg
     5041 acccgactga atgggcttaa acaatttctc gctttcgccg ccaatagtgg cgcggccatt
     5101 ttcttcctgt tttcgggcag ggttaactgg cgtgtggctg gggtgatggc gatcagcgcg
     5161 ctgttgggcg gtattgccgg agggaaactg gctggtcggg tgagtcccgt ggtactgcgt
     5221 tgggtggttg tgagcatcgg ggttgttgtg gcagtcatct tctttatcca aatttatctt
     5281 aaaccttgaa aggcggtact tttatgttga atacgaaacc gcgcctagcg ctgatcattg
     5341 cctgcagtat gtacctggca tttggtatgt tcaacgctgc tatcggaccg gtactgggtg
     5401 aactggctga aaacacccat tctacactgg taatggtagg gggtgtcatc acgtttttgt
     5461 tcctcggctc gctcgccaca cagcttatcg ccgggccaat catcgataag tttggcctgc
     5521 ggattgtggg gctggtatcc ctgttgctgg tcgcgttcgg gctgcccgcg tttaccaatg
     5581 tccactctta cccgctgatg ctggcgctgg ttttattgac cggtctgggt caggggggaa
     5641 tggatttgac cgccaacctg ctggttgcag acgcctaccc caataaatcc acgcctatgt
     5701 taaacctgct gcatttcttc ttcggcttag gcgctttctt cgggcccgcg ctggtggggc
     5761 tggctattgc tgcaaccggt tcaggcatga tcattcattg ggtggcagcg gggatgttcc
     5821 tgctgctggc cctcatcctg ctgcgtgttc ccgcaggcaa agccccagcc ccggatgctg
     5881 caaaaaaacc tgatgcaaaa gtgaaggacg agaagagcat ttacctttct cccttcttgt
     5941 ggaccatcgg cgtgctgatc ctcatttacg tcggcacgga gtatggttta ggcagttggt
     6001 cgacccgtta catgggtctc actactggca tggctgatca aaacggagca ctggtaacct
     6061 ccgcttactg gggcgcgctg acccttgggc gccttgccgg cgccgcggcc agccgccggc
     6121 tcagcaacac gcgcctgctc ggtgtttcac ttggtggcag tttactcggc accttgggtt
     6181 tggttctcag ccgcggttcc gttctgagca ccgttgtttt catcgtcatc accggtttct
     6241 cgctcggcac ggtttatccc acatctgtag cggtcaccgt ggagaagttc ccgaaagacc
     6301 aggggaaaac tgtggggctg ttggctgcca tgggcagcat tggcggcctc gccctcccct
     6361 ggctggcagg gttcttactt gaaaccactt cagccttggt tttcgccctc ttcttatgcg
     6421 ccaccacagt tgccatgttg ctgctgcttt ttgtgctggg tcaatacaaa aaggggacat
     6481 cgaacgggta aaacagtgaa caagaggaaa tgaagtacgc tcttgccact gtgagggaga
     6541 aggcaagggc tttacgcgga tttatgtttt cgtctcgata tgccgaacga ccagcctgac
     6601 gccttcgacc ttttcaacga tcactttctc cccttttttt agggggggtt ctccgggtgc
     6661 caacaccgcg ctccactgtt cacccgccac ctgtacgatc ccgatcggag ccaggcgtgt
     6721 aactacatag ccatcttttc cttcgagcga ttcccgcccg gtgatcacag gttttttcat
     6781 cgcccgcagg gcgatcatca caaccgcgaa gaaagaaagc cccaggaaaa ctcccattcc
     6841 gatgaccaac ccgaccgaaa ccctgggaaa ccccggtatg gaaagcgaat taaacaggat
     6901 cagcgcgccg gcgatgaacg aacccgtacc agccacagtg agcgcgccgt gcgtcggcgc
     6961 tttgatatcc aggatgaaca gaacaaaagc aataaccaag aaaatcatgc caaaccagtt
     7021 caccggcagg atacccagcc cataaatcgc cagcgccagc agcaccgccc caataaaccc
     7081 cgccacccat ccaccggggc tggaaatctc aatcaggatg gcctgcaccc caatcgaaag
     7141 tagcaagaaa acgatattgg ggtttgtgag caaacccagc acatcttcta ccatggtcgt
     7201 gctcactggt acctgaagcg cgcctttcga gcggatgacc acctcgttgc cattcatcag
     7261 caccttgctg ccgtccattt gggccagcag gtcctccacg tcgcgggcga tgaaatcgat
     7321 caaccctgcc tgcagcgctt ccgttgagtt ggccgctctg gctttttcaa tggattcttc
     7381 cgccagcttc accgcgtcag ccccgcgccg ggctgccagt gagcgcgcgc tggctttaag
     7441 tatttccttg gtcttttctt ccagggtgga cccgatatct tcgccctgca tgcctaccgg
     7501 gctggctgcg ccgatggtcg attcaggcgc catggcggcg aaatgccctg agagcaccaa
     7561 cagcgtgccc gcggatgccg ccatcgcccc gcgtggggta acatacacca ccaccggcac
     7621 cgggctggcg aggatctgct gcaccaggtg gttcatcaaa tcgacgctgc caccaggggt
     7681 gttcaattca ataatcagta acccggctcc agtgctcacc gcccggttta ccccgcgctg
     7741 cagataaccg ctccaaaccg gggtgagcgg tcctttaagt tgcaccacaa ccgcttctgg
     7801 cgcgtccacc tgcccgctga ccagctgaag cggttgaaag aagctgacca aaatggacag
     7861 aatgagactg acctgtaaaa accatcgcgt tttcatgaag tcgcgctcct ttaaagatta
     7921 tagcacgcgc ccacccccgc tgtgcctctc caatagtcct cctatgaaat gttgatactt
     7981 tattaataat cacctgctat tctagacctg tatgaaaagt atctggttac aaggagaaat
     8041 acaatgggaa aaataattgg tatcgatctg ggaacaacca actcagtggt agccgttatg
     8101 cagggtggag acgcccaggt catcaacacc gctgaaggcg cgcgcctgtg cccttctgtg
     8161 gttgctttta ataaaaatgg cgagcgcctt gtggggcaaa ccgccaaacg acaggcagtg
     8221 atcaacccgg aaaacacagt gttctcgatt aaacgcttta tgggacgccg attcgatgag
     8281 gtggaaagcg aacgcaagat ggttccattt gaggtgatct ccggtccgat gaaagacgcg
     8341 cgtgtgcgca tccctgtgac aggccgcgat tactcacctc aagagatttc tgccatgatc
     8401 ctgtccaagc tcaaagcaga cgccgaagcc tacctggggg agaaggtcag ccaggcggtt
     8461 atcaccgttc cggcgtattt taatgacagt cagcgccagg ccaccaaaga tgccggtaag
     8521 attgccgggc tggaagtgct gcgcatcatc aatgagccta ccgcttcagc cctggcctac
     8581 gggctggata aaaagaagaa cgaaaagatc ctggtctttg acctgggcgg gggtacgttt
     8641 gatgtatccg tgctggaggt aggtgaaggt gttttcgaag tcaaatccac cagcggtgac
     8701 acccacctgg gtggcgacga ctgggacgag gttatcgtca actgggcggc tgatcagttc
     8761 caggcggacc agggaattga tctgcgtaaa gatcgccagg ctctgcagag attgcgagag
     8821 gctgccgaaa aagccaagat cgagctctcc acagtaatgg aaaccgagat caacctgccc
     8881 tatatcaccg ctgacgcaaa cggtcccaaa cacctggtca ccaaactcag ccgttctcgc
     8941 ttcgagcaaa tgaccgcgca tctgctggat cgctgcaaaa aaccgtttga gagcgcgctg
     9001 aaagacgccg ggcttaaagc atctgacatt aacgaaatgg tgctggtggg cgggtccacg
     9061 cgtatgccgc aagtggtgga tatggtgcga gggctgatca acaaggaacc taacaagggc
     9121 gtcaacccgg atgaagtggt cgccatcggc gctgccattc agggcggcgt gctcggcggc
     9181 gaggtgaaag acatcttgct tctcgacgtg actccgctct cgctgggcgt ggaaacaatg
     9241 ggcgcggtga tgacacccat cattgagcgc aacaccacca tccccatccg caagtctcaa
     9301 atcttctcaa cagcggaaga tgggcagacg gcagtggata ttcacgtact gcagggtgaa
     9361 agacccatgg cggcggataa tatgaccctc gggcgcttcc ggcttgaggg gatccccacc
     9421 gctccgcgcg gtatcccgca aattgaagtg acctttgata tcgatgctaa cggtatcctc
     9481 aacgtcaccg cgcaggataa agccacgggt cgtgagcaga aagtgaccat caccgcctcc
     9541 accaacctgc agacgtcaga tatcgagcgc atggtggaag aagccaaagc gcacaaatct
     9601 gaagatgacc gccgtaaaga gctggccgaa gctcgcaaca ttgccgacaa cgccgcctac
     9661 caggtcgaaa aggcggtcaa ggaagccggc gaaaaggtaa cagccagcga tcgatcgtcc
     9721 atcgaagccc gggttcagga actgcgcagc gcgatgaccg gagaggatgt ttcgcgcatc
     9781 aagagcctga cagaaaccct gcagcaggat tacgcccgcg tgatgcaggc ttctgcccag
     9841 gcgcagaccg cgcccggcgg tggtgatgcc ggcggcagcg cgccacctga cgacggcggc
     9901 gacgtggtgg acggcgaatt caccgaaaag aattaacctg tttnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnngacgacg gcggcgacgt ggtggacggc gaattcaccg
    10081 aaaagaatta acctgtttaa cagaatcaag ccaaaaggcg gccagttggc cgccttttgc
    10141 ttcgcgccag gtgccattga ccaaccagcc catcccccag cgcaacccaa caacgctgcg
    10201 ggctaacgcg cagtatatac atgttagaat tcagccattc ttattattct caggccaaca
    10261 tgagcgcttc tgtcccctca acctttactc ctacccccat cgtccgagtc gcggagcgcc
    10321 tgctcaccgc cttcctcaag gtggccgcga agcagtatct ttctcttcaa ccaaccaacc
    10381 tgtcctctgt cccgccgccg cagcccggac atcgttacac tttatacgcc cacgtgcctt
    10441 tctgcgaaag cctctgtcct tactgctctt tcaaccgctt tgtgttagac cgggagaaag
    10501 ccgcggctta cttcaaatct ttgcgcgcag aaatgcgcct ggtcgccaac ctgggctacc
    10561 gtttttcttc gctttaccta ggcggcggca cccccactat cgtcccagat gaactggcgc
    10621 gcaccattga cctggcgcgg gagctcttca gtatcaacga ggtcagctgc gaaaccaacc
    10681 ccaaccactt gaacccggag atggtcacgc tattgaaaga gcgtgtacaa cgcctttcag
    10741 ttggcgttca gagtttcaat gacgggctgc tcaagcaaat gaaccgctat caaaaattcg
    10801 gttccggcgc cagcatcctc gaaagcatcc gccgtaccgc tccacacttc aaatccctca
    10861 atgtggatat gattttcaac ttccccagcc agacgccgca aattttgcgc gatgatctgc
    10921 acgcggtcat cgactcaggc gcgcagcagg tgacctttta cccgctgatg tcttcaccct
    10981 cggtcaggcg ttccatggcc agcacagtcg gtgaactcat ccctgcccgc gaagccgatt
    11041 attacgccat catcagccag ggattagcac aggatttctc cgcgctctcc gcctggactt
    11101 tcgcgcgtaa tggcagcaac ctgattgacg agtatattgt ggataacgaa gaatacgtgg
    11161 gggtgggatc cggcgtattt tcatacctga atggctgcct ttacgccagt acattttcct
    11221 tacaggaata cagcgacctg gtttctcaag gccggatggc gctcagcggg gcacagcctt
    11281 tcagccggcg ccagcaaatg cgttacagga tgatgatgga catgttcggc ctgcgcttcg
    11341 atcgcgccgc cttcaggcag cgctttggtg ttcctgccag ccgcgggcta tggctggaaa
    11401 tgctcttctt ccgcctgctg ggagcgtttg agaagaagaa tcctgccatc cctactcccg
    11461 ttgggcgcta cctctcgctg gtgatcatgc gcgaattctt caccggtgtc aaccaggtgc
    11521 gtgatatcgc gcgcgcagcc ctttccccgt acgaccgcgg ggaatgttac gcgccctgaa
    11581 agttgagaaa ccccgccgga aagccgcagc gagcgcacag gcttacccag cgcctgccgc
    11641 cagcctttga cgcgccgggc ttcaccatcg ccgagcacgc caaagggagg ttctatcttt
    11701 ccaatccatt caataaagag ctggaaagaa ggttgaccat tgatggtgct ggcgcatttt
    11761 ttcctgcctc cctcgggttc acagttaacc agcaagaagc acaacaaacg gcaagacccg
    11821 cccagcaatc agtacgccga aagcaacagg gtgttgctcg attaaccatc gttgagacaa
    11881 tcatctgact gtttgactgt ttgttcgccc tgttcctggt ataagccctg gccgtaataa
    11941 ctgaagagag agtgcatatt cagatgttat aatgctcgca tgcgttacag gcgaaaatgg
    12001 ctactcctta tactggttgt gttaatcgtc gccggtctaa ccctcagcct tcctcccgtt
    12061 tggtcgcgca tcacctatca tacccacaac ctgttcgcgg acattaaata tcgtctcaat
    12121 cctcctcagg ctgaggtttt tgttccttct ggcgctgaag aaggaaattt gattgcgact
    12181 gcttccgcag caacactgac ggcgctcgtt cccacaccca cttcacccat tctgacaact
    12241 gaagaaactc gtctcccctc ttccacgcct actctcacac ctgtacccct gccaatcaaa
    12301 gtctacctgc ctggagttaa acctgaaccc cagttatgga acaactgcgg accagcaacc
    12361 ctttcaatga atctctcctt ttatcaatgg ggcaaaactc aggtggaggc tgcggaggtg
    12421 ctcaaaccca atccgcgcga taaaaacgtg atgccttacg agatggtcac ttttatcaac
    12481 acacagacct cgctgcgcgc gctttcacgt atggggggcg atgtggagac cctcaaacgc
    12541 ctgctcaacg cgggcttccc ggtgttggtg gaaaaaggtt ttgagcccgg cgatctgaaa
    12601 aaagaaggct ggatggggca ttttaatctg gtcattggtt ataacgatga tgctcagtcc
    12661 ttcattaccc aagattctta cctgctcagc caccccccgg ggggaggcga gatcaaagac
    12721 ctcgaaacct tcccgggttt cactgtgcca tacgaagata tgcaaaagga ctggcgcgcg
    12781 ttcaattacg ttctgattgt tgtttacccg cccgaaaaag agaatgatgt gctcaatgcc
    12841 cttggtccgc tgatggacga gaatggtgct tatcggttgg cctatgaacg cgcaaaaatg
    12901 gaagcgacca cactgagcga tatacgcgaa cgctttttcg cctggttcaa cgccggcacc
    12961 agcctggtgt acctggcgga ttacaatggt gcggctgcgg cttatgattc cgctttcaac
    13021 ctttacgcgc agataccaga gaacgcccgc ccctggcgta tgatgtggta ccagaccggc
    13081 ccttacttcg cctattacta ttccgcccgc tacacagatg tgatcaacct ggctgaccag
    13141 acgctgaaac gcatgagcgc ggaacctatc cttgaagaaa gttactactg gcgcggcatg
    13201 gcttacctgg cgctgggaga taacgagcgc gcgcgcgccg agtttcgcga cagtcttaag
    13261 tatcacccgg ggtttggccc cagcctggtc gcgcttgaac aaatggggga aactccctga
    13321 gattttcggc tatacttgta atggttagaa aatttcatga tcaagatcct gcattttgct
    13381 gacgctcata ttgatattgc cacacacgga aaacatgacc ctgaaaccgg cttgcccatc
    13441 cgcgccctcg actttttaaa agccctggat accatcattg accacgccat ttccgaaaaa
    13501 gtggatctgg tcatattcgc cggagacgcc tacaaagacc gaaccccatc gcccactttc
    13561 caacgtgaat ggggaaaacg catcatccgc ctgccacagg cggatatccc cgcactttta
    13621 ctggtaggga atcacgatat cagtcccgcc agcggacgcg cgcacacctt gcaggaattc
    13681 gacaccctgc aggtgccgaa agtgcgggtg ctttacaaac ccgagctgct taaacccgcg
    13741 gacctgtggg ggctgcagtt gcaggtgctc tcgctgccat gggtgttccg ctccagcctg
    13801 gtggccgcgc tggggctttc cgctgtagaa gaagacactg ttcacgagga gatcgaaaaa
    13861 cgcctcacca ccatcctgca ggcgcggatg gacgaacttg accccgcgct gcccactgtg
    13921 ctggtggggc acggctccgt gcagggggca gtttatggca atgaacgcac cgtcatgctg
    13981 ggcaaagacc tggttctgcc tggctcactg gtgaaagacc ctcgcctgga ttacaccgcg
    14041 ctcggtcata ttcacaaata ccaggacctc aaccctgggc tgcacccgcc agtcgtatac
    14101 gcaggttcca ttgaacgggt tgatttcggc gaggtcgcag atgacaaggg ctttgtcatc
    14161 gctgaggtgg agaaaggaaa cacctcgtac cggtttgtaa aattgcacgg tcgcgccttc
    14221 tttgaccggc tggtcaggct ttccatggaa gacagggcga tggaaaaaat cctcgcttcc
    14281 tcaccgaacg aagctgaagc cgagaacgcc atcttcagac tggtcattga gtatccgcgt
    14341 gagcttgaag ccgatattga tgagcctgcc ttacgccaaa aattcgcctc cgcgctcgaa
    14401 tttcacctgg tccgccgccc gcaagcagag gcccggctgc gcctgcccgc tgatcagaac
    14461 atcgccagct acagtccgtt ggaattgctc tcgacttact ggaaatccac tcacgcccaa
    14521 cctcgtgatc ttgcagggct gcaaacactt gctcaatcga tctttcaatc cgtaaccgga
    14581 tcaaagcccg ggtaaggacg gcaaggttat ccgacatgtc caatacattg attgaaatcc
    14641 tggtcatttt cgtcctgatc ttgtttaacg gttttttttc tatgtcggaa attgccattg
    14701 tttcagcccg caaagtgcgc ctggaacaac gcgcggatga aggagatgcg ggcgccggtc
    14761 gcgcgctgca gctctccaag gagaccgggc aatttttgtc ctccgtgcag gtgggcatca
    14821 ccctggtggc gatgctcacg ggcgcttttg gcggcgccac tctggcagag cggctgaccc
    14881 agtcgctcgc ccgcgttccc tggttggcca cgtcggccgc cacgctctcc ctggtcattg
    14941 tcgtgctggt caccacttat ttctcgttgg tcattggcga gcttattccc aagaagatcg
    15001 cgctcaacaa ccctgaaaaa atcgcttcaa aagtctccgg tatcatggtg tttttctccc
    15061 ggctcacatc accgctcacg cgcctgcttt cttcctccac tgacctgggc atgcgcctgc
    15121 tgggcatcaa gccctccggc gaacccccca ttaccgaaga agagatcaaa gtactcatcg
    15181 accagggaac acaggtgggg gtatttggtg aagcagaaca ggacatggtg gaaggcattt
    15241 tccgtttgaa cgaccgttca atcaatgcca ttatgacccc gcgcacggat gtcacctggt
    15301 tggacgcgga cgacagtatc gaagaaatac tgaagaccat cctccaatct ccctattccc
    15361 gctttccagt ttccagggag tcattggaca atgtgctggg aattctcaaa tccagagact
    15421 tgcttgaaaa gcagctttca ggcgaacctt ttaacctgtt ggacctgctg caaaagccac
    15481 ttttcatccc agaaaacacg cccgtcatgc gcgttcttga aatggagcgt gagcacgggg
    15541 tacatgaagc cctggtgatc gatgagtacg gtggcgtgct cggattcgtc accctttttg
    15601 acgtgctcga agccattatc ggtgaactcc ccagccagga ccaggcttca gaacccgaaa
    15661 tcgtgatgcg cgaagatggt tcttacctga ttgatggttt actgcccatt gacgagttta
    15721 aggacctgtt cgatctggac ctgctccctg aagaagataa agtgggattc caaaccgctg
    15781 gcggattcgt gatgaaccag gtgggttcca ttccccacgc cggtcagact ttccaatgga
    15841 atgatctgct atttgaggtg ctggatatgg acggcctgcg tatcgacaag atcttggtca
    15901 gccgcaatag cgaaaaaacg ccgcctgctg gaggggataa atgatcatcg accgcctgca
    15961 aatcagcggg tttctttcat ataaagagaa agtggatatc gacttctcag gtttcgccct
    16021 ggcctgcatt tcaggtcaaa acggcgccgg taaatccagt ctgcttgaag ccatcacctg
    16081 ggcgctcttc ggtgaagcgc gccgtaaaga cgacgccatc atccacatgg gtactgatac
    16141 cgccgaggtg ttgcttgagt tcgattacga gtccacgcgt taccgggtac agcgcagcaa
    16201 aactcgcgac aagagcacac agcttgagtt tcaactgcgc aacgctgaag gcaactgggt
    16261 gccgctcact gaagccacca tgcgcggcac tgaagagctc atccgccgca cccttcacct
    16321 ggactatgaa acatttacca atgcctcgtt tttcctgcag ggcaaagcag accagttcgc
    16381 tcagcagcgc ccgggtgacc gtaagcgtat cctatcaggc atcctcggtt tggacatttg
    16441 ggaggactac cgcgaggaag cagcgcggcg ccgtaagctt ttggaaaatg accttgccct
    16501 ggttatcagc catttacagg aaattgagac tgaactgcaa caggaagcag aacgaaagga
    16561 acgtctcgcc cgcctggaaa aggaagtgag cacccagacg gaactctttg aggcgcgaaa
    16621 agcctatctt gagcagcagc gccaggttgc cgagcgtatt ctcgccgacc aaaaacaggt
    16681 agaaaaacaa cgcgcggaga ttaatcgctt gcgcgcagag gatgaaacga tgcgcagcga
    16741 tctcgacctg cgcacgcaag aacgtcagac ccatctttcc ctgttacagc aggaaaaaga
    16801 gatcaggcag gaggtggacg cctgggaaca ggcgcgtaaa accctcgaag agtgggacac
    16861 gctggcgcag gattttcacc agtacgattc ccagcgtcag gccccgctgc aggccatcgc
    16921 ggtagaagaa agccgcctcc aggccgagtt aaactcgctt caacagaaac aggctgaaat
    16981 cttctcaata gaacaggaac tgcctgagat ccagcggctg gttgaagact ccagcaaagc
    17041 agtcgaagac tgcagcgcaa agctggcaac ccgagcacaa ctggaaaaag aaatgcgcgc
    17101 tctcctggat gaaatcgccc gcgcccgggc tgaaaacacg cgcctaaaaa tggagatgga
    17161 tgaaattgtt gagcatcgca ctcacctgga gaatatcagc ggcgctaact gccccacctg
    17221 tgaaaaaccc ctctccgacg atgagcgcca aaggctggtg gatgagatca acgcgcgcgg
    17281 caaagaaaag ggcgacgcgc atcgcgaaaa taaaaggata atcgaacaaa gcgaagccca
    17341 atatcgtgag atcgaaaccg cgctcctcaa cctgcagcgg gtggacgccg agttgaaact
    17401 gcaacaacgc atcttcgaca cgaaagcgca ggaattacgc atgaaggaag ccgcgttcac
    17461 tgactggcag caaaaaggcg ccgtgcgcat gttggagctg gaagagacgt tgcgctctca
    17521 atcctttgcg ctggaagcgc gcaagcagtt agccatgatg gatgctcaat tgaagaaact
    17581 gggttacgac gccgccgctc acgaacaggc gcgccgtatg gaactggatg gccgcgccag
    17641 ccaggacaaa aaaatacagc tggagaaggc caggtccgcc ctcgcccccc tcgaacaaaa
    17701 tatcacctcg ttgcaaaaga acatggagaa caaccaaaag cgcctttcag cgctgcttga
    17761 tgagctcagg caggacgagg aaaaactgga gatcgaaact tccaacctgc cggatctcga
    17821 tcaattggaa agagattact acgcagccca ggagcagatg aaccaggcga acagggattt
    17881 agggtacacg cgcaatcagg tggaagtgct ggaaaagcag cgcgaactaa aggtggagaa
    17941 actcgaagag aaagaccagc ttaacaacca ggtcactgat ctcaaaacgc tggaacgcgc
    18001 ttttggcaaa gacggcattc ccgcgctgct catcgaacag gcgctgccag aaattgagcg
    18061 tcacgcgaac gagatcctcg atcggctaag ctcagggagt atgtctatca gcttcgaaac
    18121 ccagcgtgat ttcaaagata agaagcgcga agaacgtcgt gagacgctgg acattctcat
    18181 ccgcgacccc gccggtgaac gcccctacga gctcttctct ggtggcgaag cctttcgggt
    18241 caattttgcc atccgcctgg cactcagccg tgtgctggca catcgcgccg gggcgcgcct
    18301 gcagaccctc gtgattgatg aaggttttgg cagccaggac gctgacgggc gtcaacgcct
    18361 ggtagaagcc atcaacctgg taagcacgga atttgagaag atcctggtca tcacccacct
    18421 cgaagaattg aaagacgcct tcccggcgcg tattgaagtg accaaaacgc cggctggttc
    18481 taaggttgag gtaatcgctg tatgaacatg attcacgccc gtgtcgggtt gcagcagcgt
    18541 gtgctgccgg agtaccgcgc ggaattcttc gacacgctgg ctaccgcctg cctgcagggg
    18601 ttgggggttt tcgcgggtga gccctgccag ggtgaagccg tagaagttgt tgaccagctc
    18661 tctattgcct actacacaca cgcacgtaac ctgcattttt ttcgcggcag cgcctatttt
    18721 tactggcagg caggggtttt aggctggctg cagcgctggc agcctgacgt gttaataatg
    18781 gaaatcaacc cgcgtaatcg ttccaacccg ctggcggcgc gctggatgaa agaacgcgga
    18841 cgcccggtgc ttggctgggg gttgggttta cccggcgccg gaaagatcta ccggcttatc
    18901 taccatagcc ttttacgttc ctgcgacgct gtgatcgcct actccaccac cgccgccctc
    18961 cagtaccgtc atgccggagt ggacccatcc cgcgtatttg tcgccgctaa cgcggtcacc
    19021 cgccgaccaa cggacctggt cgttgcacga ccgccgtcct tccatgaaaa caccccgcag
    19081 ctcctcttcg ttgggcgcct gcagccgcgc aaacgcgttg atacgctgct gtacgctttg
    19141 gccatgttgc cccctcacca acaaccgcgg ctatccatta ttggagacgg acctgaccgc
    19201 tcacgcctgg agatgctcgc ctggcaggtc tatccacgag ccgtgttcca tggtgccaaa
    19261 cgcggtcagg aactggaccc ttattaccag gccgctgatc tattcatcct tccgggaacc
    19321 gggggactgg ccattcaaca ggctatggcg catgccttac ccgtcatcgt aggcgcggcc
    19381 gacggcaccc aaattgaact ggtgcgtgag gaaaacggct ggacgctgcc ggatgacagc
    19441 ccggaaaccc tcgcccgtgt tattcgagaa gcgctgtcag atgttgagcg cctgcgtcaa
    19501 aaagggctgg catctcgtcg tattgtttct gaagaggtca acctggaagg catggtaaag
    19561 accttttgca cagcggttga atccgttctc tcaaaggctt agaggaggaa agcgtgaacc
    19621 tgttgatcat tgcagatggg cgcagcccaa ttacccggcg ctggatacgc atgctgcagc
    19681 cgctgaatta cactatcaac ctggtctcta cctacccctg tcaaccggtc gacggagtga
    19741 acgaaaccat cctcttcccg gtggcgttcg ccggactatc cggcagccag gcgggaggcg
    19801 gcgccagggg cgggcgaaaa gtagtttcgc gttaccgtgg actcgctgcc accctgcgcc
    19861 actggttggg tccattgacc atcacccatg cggagaaaga ttatctcgag ctgctgaacc
    19921 gcctgcagcc agacctcgtg catgcgctgc gcatccctta tgaaggcatg cttgccagcc
    19981 gcaccccaac gggcattccg ctggtcatct ccacctgggg caacgacttt acctatcatg
    20041 cgatctcaaa tggggtgatg ggcagcgcca ctcgccgcgc gctgctgcgc gcggatgggc
    20101 tgatttctga taccgtccgc gacctgcgca tcgcgcgcgc ctggtccttc aaccccgcca
    20161 agccggctgt atctgtaccg ggtaacggag ggatcgacct ggctgagatc gagaacatca
    20221 cccaggggat cgacccagct gtgccctggc aagtgatcaa cccgcgcggg ttccgcagcg
    20281 gctccgtgcg caacgacact ttttttaaat ccatcccgct cattcttagc ctgtacccag
    20341 aagtgcagtt catttgtcca tggatggcgg gacaacccga agccctgagc tgggtgaatc
    20401 gtctcaggat actggataac gtttccttgt taccgatgat ctctcaggct gaattatggc
    20461 gcgagttcgc tcgctctctg gtatccgtct cggtcagtga gcacgacggc acgcccaact
    20521 cgctgcttga ggccatagcc atcggctgcc tgcccgtgtg cggggatatc gaatcaatcc
    20581 gcgagtggat ccgcaatggc gagaatggta tcctggtcga tcccgctgat gcttacgcgc
    20641 tggccgaggc tgtgcagcgc ggaataatgg atgaagaatt ccgccgcgct gccgctgctc
    20701 gcaaccgtga gatcatccgt gagcgtgccg aaattaccat cgtgaggcaa aaggttgccg
    20761 agttttacca acgcatgacc gggttcgtgt aataaaaaca ggctggcatt ttgccagcct
    20821 gttttttgtc tcgctaaagg ttgtggatga ccccctcttt tttatacctc ataaataact
    20881 gcccccagcc ggtgttttgg caccattcct taaagacata tttttctttt aatggccagt
    20941 gcacataatc atgatacacc tctgaaaagg cgatggggat ggccaccagc ggtgtacggg
    21001 taaccagctt ttgcagaaac ttggtcggac cataccaggt aagccagccc atgaactgat
    21061 gcagattgtg acccactttg aagtgccagt tctctcctga aatatcatcc cccaccaact
    21121 cgatctcacg aggatcgccc actcccaacc cctggttgtg accaaggttg atgtacttga
    21181 tgctcatcgg gtcaaaaccc atcagtttgg ctgccactgc atcaatggcc acctggtctt
    21241 gtgatgccaa aatgacattc ttcacttccg gcttcatcac ccgcggaccg gtgccattgc
    21301 ccgcggtggt gccgtccatc accgcgaaca atcctgtgtg gatttctttc tggatagcca
    21361 gtaggtcgat gatggtctcg tggatatatg aatgggtgta atgtcgatgc ttggtgagca
    21421 gacctccaaa tgcattctta atggcgccgg tggtggtggt gtagatatgg catttcgccg
    21481 tcggcagatg cacgatgttc tttcctacaa agtaatctgg aatgtagata ccttccggat
    21541 agatatggtc gagggcattc atcttcgcct tgggcttgta cttaacccag gtcatatctt
    21601 tgtcttcaaa gttatacagc accgggatat ggtagtgttt gaagatggga acgtaaccgt
    21661 tgaggtcttc tcctttgaaa gcgtttgtca ccacggtctg gttttgcaca caggtaatat
    21721 cattgtagcc tttacttttc agcgccagga tggtgccttc catctgccag ggtgtggtgt
    21781 tggcgctggg catcggaaag tgccaagaga tattatcttt gaggatggta gtagcaccag
    21841 ggtccagcgc tttaggccca tcagccagtt cgaaaagacg aatgtagtct tcgataacgg
    21901 tttctggcgt agtcttgagt acagcgactt ttgaattcat tctccacgtc ccttcacacg
    21961 tccttttatg ggtgaattat accaacaggc agttcgcctg gccaacccag cctgtgtacg
    22021 cgcaaccccg gctgatttgg tctataatcc ttctgagttc atgaacaaga agatgccttt
    22081 gaaacaaccc ggctggaatt tccgcagtcg cgcctgcggc ctgttgattt gcggtctgtt
    22141 actgttggcg tcctgtaact ttccaatacg cgacttttcc gctttgcccc gaccttacgc
    22201 gaccttggat ccagcgatct tcgaatacca tcgttctgcc acacctactg ccgggcagcc
    22261 tggcgctgct cccacccgca cacctcagcc tccgcccgtg gtggatgaat cactgtacca
    22321 tatttacacc atccgttctg gtgacacatt cgaaacggtt gccaggcact tcagtgtcac
    22381 tcccgctgct atcatcagca tggaagcgct gccccaaacc ggaatcctcc ctccaggccg
    22441 ttacctggtc attcccaaga acaccgacaa ccttttcgag caagagatga ttttgcctga
    22501 cagcgcggtg atcaactcgc cttgcgccgc gccttttgat atcagcgcct atgtaagcgc
    22561 tgccggcggc tacctgagca ctttcagcca gcccgtggcg ggcattcgct caagtggggc
    22621 ggaaatcgtg cgccgtgtcg ctgataatca atccgtaaat ccaatgctgc tgctggcaat
    22681 ccttgagcac cgtgcgggat tggtttttgg aaaacaacca cctgcagata ttaatcaccc
    22741 tttgggtttc cgtaacatgt actacaaggg gctttaccag gagttgtcgc tggcggctcg
    22801 tatggtaaac accgggtact acggctggcg ttatgggacg atgaccatgg tcacttttac
    22861 tgaccaggtt tcgcagcgga tctccccgca cttgaacgca ggctcagttg ccctcatgta
    22921 tcttttctcg caatcgtatc cctcaacaga ttggaacgag agattgcttg gaaaaagcgg
    22981 atttatggaa acctacctcg ccatgtttcc agacccctgg ttgtgtgccg cagaagttga
    23041 gccgttgtta agtgacacgc tcagcgcgcc gcagttagag cttcccttcg ccccgggtga
    23101 agaatgggca tttaccgccg ggccgcatta cagctgggtg gagggcaccc ctttgggcgc
    23161 gcttgatttc gcgcccaata ttaaaagcgc tggctgccgg gtctcgcctt cgtgggtgcg
    23221 cgcttcagcc ccaggggtga tcacccgggc ggataacggc gtggtgatat tgacccttga
    23281 ggatgaaaag ggtgacgtca ccggctggga agtgatgtat ctgcacatcg ccgcaaacga
    23341 gcgcgttcca ttgggaacac gcctgcgaaa agacgacccc attggtcatc catcctgtga
    23401 aggcggctct tccacgggaa cacatgtgca tatcgcccgc aagtacaagg gcgagtggat
    23461 tggcgcgcaa ggactgttcc catttgtatt aagcggctgg gtggcttacc ccggggaacg
    23521 cgcctaccag ggccggttga tgaaagacgg gcaggtggtc atcgcccgcc aggggggcaa
    23581 ccaggactct ctcatcaaac gttagtattc caggtgtgga tggttggttg gggaaattaa
    23641 aataaaaaag gatggtttcg cgtctccgac catcctttca aataaccaat gcgactgttt
    23701 aatcggcttc gcgcatgcca agctggtgat caagctggta cagacctccc aggctgcctt
    23761 ccagtttcgt caccatcgcg agaacagagg aagcattctt ttcttcttcg acttgttcat
    23821 tgatgaacca ctgtaagaag atctggctgg ccacatcatt ggcttcaact gccttggcgt
    23881 aaatgccatt aatcagcccg gtgactttct gctcgtgctc atacaccttg gtaaatacat
    23941 ctttcacgcc tttaaattcc actggtggtt gttcaatggc tttcaggctg atcttctcgc
    24001 cacggtccag caggtagtcg tagagcttca tggcgtgctc ctgctcctct tctgcctgta
    24061 ctttcagcca tttggcgaaa cctggcaagt tttcggtttc gcaataagcc gacattgaga
    24121 gatagaaata ggcagaatac agctcgtgtt taatctggtc gttcatcgct tcatatactt
    24181 ttgcattcat aagacaccct ctctgacgaa gaattattat ttttacctgt taagaataac
    24241 acaactctgt ttgaaatttg ttaatttttt atgaccctgt aacgtttgag tttttaagta
    24301 ttcggcgaaa acctatatat gtgcgccttt caagaccgct taaacgcgat tgctttaggc
    24361 gcagtgttgg cagaaaacct ctgcatgctg ctggagttcc tctcgacgcg tgctttacaa
    24421 gacctcgaaa ccgagatgat agacaagaca ctgccagcgg gtgcttctcc gcgcctgggt
    24481 gtggcagtat gccaatccgc ataaaatgga tggtaagcag gctgattgga taatccgcta
    24541 gaaaaaaaat ggtctcttct gctatcttta agtttaaaga agggtgggct caacaggggt
    24601 taagacccag tcctactcac cgtacaggac cgacgcctga atcgatctgg tttccatgag
    24661 atctctgtat tggaggccct tttttgtcga gtaaggacta tctcctgtct ctcatcgttc
    24721 cctggctgtt ttctgaccag cgctcttcta gtgatgctga tttggcctgc tttgtctgtc
    24781 tttgagagcc ctttcactgg tggcttgcca gctttaagca ctcacttgca cccccctttt
    24841 tcggatattc gtgatggtga cttttgtacg aatagttgtg taatacgcaa ttaagttgta
    24901 ttatatgcaa atatcctata cactcta
//
LOCUS       DAOSPM010000019        24844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2014_length_24844_cov_12.723587, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000019 DAOSPM010000000
VERSION     DAOSPM010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24844
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2014_length_24844_cov_12.723587"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1193)
                     /locus_tag="PLW25_03070"
     CDS             complement(18..1193)
                     /locus_tag="PLW25_03070"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOS53502.1"
                     /translation="MFEKKTIRDIDIKGKKVLVRVDFNVPLNETGVGDDTRIRAALPT
                     IQYLLDGGAAVILCSHLGRPKGGPDPKYSLRPVAEHLSSLIGKPVKFSSDCIGPEAKE
                     AAEELKPGEVLMLENTRFHAEEEKNDVEMARQLASLADLYVNDAFGTAHRAHASTEGV
                     THFLPAAAGFLLEKEIKYLGQVMANPQRPFVAIMGGAKISDKIGVIKNLLTRADAILI
                     GGGMANTFMKAQGLAVADSLVEDDSLGIARDLFEEAGGKIHLPVDMVVANQFAADAES
                     KTLKVQDVPDGWRILDIGPETVKAFRAIIATARTVVWNGPMGVFEFPKFAEGTFGVAK
                     AVADSAAVSVIGGGESVAAIQQSGLADKITHISTGGGASLEMLEGLELPGVAALLDK"
     gene            complement(1296..2348)
                     /gene="gap"
                     /locus_tag="PLW25_03075"
     CDS             complement(1296..2348)
                     /gene="gap"
                     /locus_tag="PLW25_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOS53503.1"
                     /translation="MTTKVGINGFGRIGRQVLKAINEYHPGEVEVVAVNDLFDTETNA
                     HLFKYDSNYGILPVHIEAKENELIVDGKRIQVFAEKDPGMLPWKDLGVDIVVESTGVF
                     TDAKGDPAKDKPGAVVHIEKGGAKKVIITAPAKNEDLTLVLGVNDGWYDPAKHHVISN
                     ASCTTNCLAPAAKVVHEKFGILYGMMTTIHSYTNDQVILDQGHKKELRRSRAAGLNII
                     PTTTGAAKALALVIPELKGKFDGFSLRVPTPTVSIVDFVATIEKTITKDDLNAAFIEA
                     ANGSLKGILGYTTGEYGDPLVSMDFKGDPRSSIVDLPTNMVMGGNLVKVVAWYDNEWG
                     YSCRTADLAAMLGKKL"
     gene            complement(2410..3762)
                     /locus_tag="PLW25_03080"
     CDS             complement(2410..3762)
                     /locus_tag="PLW25_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOS53504.1"
                     /translation="MRRRRSKKFGRFIQSVLMQLRWLVPGIGIKRWVLVILAGTTLLG
                     LGLAVYLLDVYRTAPEGWWLPFIKIVALQFMPNRILRAFIFGGLGLVLIGIGITGLNR
                     ALLRPFLRPGKDVIDTVAEFRRKEKGPRIVVIGGGTGLTSILRGLKSYSSNITAIVTV
                     ADDGGSSGEIRKSIGILPPGDLRNCLAALSNDEEMMTQLFQYRFASGAGLNGHSLGNL
                     LITALTEITGSFEAAISESARVLAVQGSVLPATLKNINLVGEVELAENKTIIQVRGES
                     EISQAGGRVRRVWLDPGNPTSYPPTIQALLNADLILVGPGSLYTSIIANLLVPDLLEA
                     FKASKAYKFYICNIATQRGETDGYTCEDHVRAIEKHAGHRLFDVVLANNRYDHHMPEG
                     TEWVKVCEDDNRYPYYRADFVNVEKPWRHDPSKVAKTIIDLYMERTGPLVSKEEKSSI
                     "
     gene            3989..5299
                     /locus_tag="PLW25_03085"
     CDS             3989..5299
                     /locus_tag="PLW25_03085"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HOS53505.1"
                     /translation="MNPTRLPGQKALEIIQRDQQVISPSYPRGYPFVMDHGKGVYVWD
                     VDGNRFLDFAAGIAVNSTGHSHPDVVKAIQDQAEKFIHISSDFYHAKWVELAEYLNRI
                     APFKEDAVIFQTNSGTESVESALKLARYHTGRSQFIAFMGGFHGRTYGAVTLTASKAK
                     YHQGFFPLMNGVTHVPFPDPYRPMLHMLPGEDIGDATIRFIEKVFIHNLPPQDVAGII
                     IEPIQGEGGYVVPPDTFIPQLRELCSRHGILLIADEVQSGVGRTGRWWAIENFGVEPD
                     IVTTAKGIASGMPLGAMIARKSITTWPKGAHGNTYGGNPLACVASLETLQLIENGYMQ
                     NAKHVGSYCLDRLAEMQVRHPSIGAVRGMGLMIGIEFVMDRESREPAEKMRDAVVDKA
                     FELGLLTLGCGTSTIRISPPLCITREEIDEGLTILDEAITFAEK"
     gene            5344..6903
                     /locus_tag="PLW25_03090"
     CDS             5344..6903
                     /locus_tag="PLW25_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS53506.1"
                     /translation="MLPDFRVRQRDYLLEISRALTQELDLVKLLDRILHISLEMLAGQ
                     AGLIALRVPSEGWQIQVSTGLPPAFLRFLEPQLAQISEEHDSSDLELKTINHILGNFT
                     QSISLGRLSSVGLPLITGQKVLGAIFVFREFAAFSANDRALLASFADQAAIAVQNAQL
                     YTQVENDRRRVTGLLDSAADGILILTRALRVERVNPSLAHLVGIQPQDLTGKEHDEVI
                     RWVKPPHGMTLTQAIADGWPLTPHAALYVEGDLERTGGTRPVPVGITYAPLLSEEGKL
                     VNIITTVRDITRFREADELKATFISVVSHELKTPVALIKGYVSTLRRDDAHWEKAVIN
                     DSLQIIEEEADRLSEYIENLLDVTRLQANGFNLKRNDTQLAEIIKRVAERLQTQTIKH
                     TIIVEIPQPLPIIVADDSRINQVISNLVANAIKYAPGGEIRIKAVQRESNIIICVSDE
                     GPGINPNDVPFIFDRFYRGSDASRLTKGAGLGLFLAKSIVEAHGGRIWIDSKIGQGTR
                     ICFCLPIASTG"
     gene            6991..8343
                     /locus_tag="PLW25_03095"
     CDS             6991..8343
                     /locus_tag="PLW25_03095"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOS53507.1"
                     /translation="MPKIQTACPRCKQPITAEVQMLFDMNTDPTAKQKLLGRANNFAH
                     CQACGYEGLISTPIVYHDPNKELLLTYFPGELGFPLNEQEKQIGPLVNQVVNALPAEK
                     RKGYLFQPNSMFTYQSLIDRILAADGITKEMIEAQQKRVNLIERLLSLPNEEDRVTVV
                     RQEANLVDGETFTLLSTLIQSASAQGDEASAKLLSQIQAVLLNETDFGKKLLADSQET
                     QHALKELDKAGKEGLTREKLLEVLSSLKSETSLTTLVSLTRSGLDYQFFQILSEKIEQ
                     STDENKQALISLRDKLLDLTRKIDNEVKKRLENAASLLENILKEADVQAAIEKHAEEI
                     DEFFTQAVQVEFEKAHSENNLERMEKIQKVVSILDKMTAPPPEVVLIQKLLDASDDET
                     RKKILQENETLVNDQLLQTINAIITESSTKSQAPELIDTLKSIYKMALRAHMEKNLKS
                     "
     gene            complement(8436..9113)
                     /locus_tag="PLW25_03100"
     CDS             complement(8436..9113)
                     /locus_tag="PLW25_03100"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOS53508.1"
                     /translation="MRNSSFGNSITHEFPVLVALQGPLEGRRWIIREAIDIGRDEDCA
                     IRIEERQVSRHHARLRNENGICYIEDLGSKNGTFLSGAPLIESTRLGDGDTFQIALIQ
                     KFAFFSSDATMPIEDLDPTFLKGSGKLVLEERSRRVWIGREELLPPLSAAQFRLLATL
                     YNQAGEVVTREALIRKTWEEDEATGVSDQALDALIRRLRERLAELDQKHQYIVTVRGH
                     GLRLENP"
     gene            9209..10192
                     /locus_tag="PLW25_03105"
     CDS             9209..10192
                     /locus_tag="PLW25_03105"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOS53509.1"
                     /translation="MISPLKKGNILRERYTIRRVIGQGGMGCIYLADDLRLEGRQCAV
                     KEVEHDRTLPKNLLKEARDQFQREATVLARLDHPNLPKVSDYFSIENSDYLVMDFVPG
                     EDLRTLMLKQREKNQFIPEKDVLNWAFQLADALEYMHNQNPPILHRDIKPSNLKVTPA
                     GLLKLVDFGLVKVLAPGEVTITILQGQGTALYTPLEQYGGDIGHTDARSDIYAFAATL
                     YHLLTAKPPVDARQRFISPNALQPIRETNQAVTQKTENAILWGMALHPDDRPSTIGQF
                     REALIGSRPVAIPSGNRLSVKIPTFYNRKIGKQWYILMGLSLLTLLVTLLR"
     gene            complement(10196..12937)
                     /locus_tag="PLW25_03110"
     CDS             complement(10196..12937)
                     /locus_tag="PLW25_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOS53510.1"
                     /translation="MQKIFLLFLALSLAAPTATAQAGDRAQTNARLAAAQVRLAQMTT
                     EEKVGQVFLISFQGSSAQEGSRIVRLIQEKHIGGVVLRKDNDNFIGPENTIGVLKELV
                     TDLQAAAQMVENQESYAPLLVAITQNGYLDVDGQILHGVTNLPNLMTIGASWKTEQAS
                     AVGSVLGSELEALGINMLIGPSLDVLDIIHTEGRDDLGVKTFGGNPYWVGKLGKAYIE
                     GVQAGSANRVAVIATHFPGSGSSDRQPDEEIATVRKSLDQLMRVELAPFFNVTKIDQG
                     HIGVVDGLLLSHIRYQGFQGNIRSSTKPISFDPVAVEQIMNLPELSPWRKMGGLIVSD
                     DLGSESIRKFVDPLNTGFDARQVARSALMAGNDLLYMGNILATGDEDNYSTVVRTIDY
                     FVQKYEEDSAFHNRVDEAVLKILQLKMKLYPSFDPADVMTPRNQLETVGKAQATVMEI
                     AQSAVTLISPEMEDLNTVLPDPPGANDRIVFISNVQTNTQCSSCPVETDFPAARLMNS
                     IIRLYGSGAGDPIPASHLTAYSYENLRMVLEQADRTEDILVSLNAATWIIFSFAEFGA
                     ERVEATTYKRLFDERPDLVRNKKLIGLAFNAPYFLDSTDISKLTAYYALYSNTPEFYE
                     IASRVVMQELTPSGKLPVSVPGIGYDLVYVLSPDPTQMIPLVIETPVEIDEPQAPPLA
                     GYSQPLLYKAGDTIPIKAGVIVDHNGNPVPDGTLVRFIIDTRSTSGSVEQLEARTIDG
                     FARVMYVIPSIGSLQLSVTADPAFISQILRLDITDTGGVVTSFEPTPIPVSSDIATPT
                     EASPSPTPESKVIQLHKQGKVAFWDWLLANILIVGFCLGLSYFTHNHLSAKWNFLTIL
                     FSGAGGYAGYLWAALGLPGSKSPLNEVASGQILILVGIGLVCGFGSGFLFYWWKTRK"
     gene            complement(12965..13909)
                     /locus_tag="PLW25_03115"
     CDS             complement(12965..13909)
                     /locus_tag="PLW25_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOS53511.1"
                     /translation="MITAEWVIDAAKRIQPIIEQTPVTKVPQLDILCKWENYQKTGSF
                     KLRGASNKVLSLEKSALKGGLVACSAGNHGQGVAFAAASRGSACMVFVPENTSASKID
                     AMRQFGADVRLVRGGYVEAEKQALAHAQTSGKTFISPYNDAQVIAGQGTIGPELVEQL
                     GGLDGIRSLVVPVGGGGLISGIGVYLSNMKERPQLIGVQSETSPYAHQVYYSGTQTGV
                     TETESIAEGLAGEIAHDSITIPLMRRFVDDIVLVSEEEIRSAIRFCWKKYGERIEGSA
                     AVGLAARLAGKIQLLPAALILTGGNIQTEIFNEIIHSK"
     gene            complement(13906..14652)
                     /locus_tag="PLW25_03120"
     CDS             complement(13906..14652)
                     /locus_tag="PLW25_03120"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOS53512.1"
                     /translation="MLDDLPKPVVFAHRGASQYAPENTLAAFELAVSQGADAIELDVK
                     LSADGKAVVIHDQTVDRTTDGSGRVNQMTLASLKGLNAQYRFDAYAGEQIPTLDEVFE
                     SVGKRIFINVELTNYASRHDNLVPIVAEIVKRHQLEKEVLFSSFIPGNLKKMKILLPD
                     VPVALLASQGLLGVASRSALFIQLSPKIIHPYLDDVTPQLMNTERRRGRRVHVWTVNE
                     DNDLRRMIDYGVDGFFTDDPAKALRILERK"
     gene            complement(14653..>15936)
                     /locus_tag="PLW25_03125"
     CDS             complement(14653..>15936)
                     /locus_tag="PLW25_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS53513.1"
                     /translation="SSHFSPNPINTIEDTGLSALFLQDLALKILYFQGFMTGYKVAEA
                     LCLPFTGMVDQLLDALKRDKLVEVKSSQMGLGEGAYLYAITGAGITHAREALDRCQYA
                     GPAPVPLEAYNESVLRQSRGRMFVTSRNMRQIMGDLVFGDEVFHRLGPAVNSGTSIFI
                     YGAPGNGKTSVARAIGNQVLTQTMYIPYAIFVDGQVITMFDSMNHQLAAEDETGPQNR
                     GMKGRKDQRWVKIQRPFIMVGGELTLTGLDLVFNETLKFYEAPFQVKANGGILLIDDF
                     GRQQVRPRDLLNRWIVPLENRIDYLSLHTGRKIEVPFDVLVMFSTNLPPKDLVDEAFL
                     RRLRHKIEITDPDAVHYREIFMRVAAQKGVVFSDEGLNYLLKEWYAKHNRKLRASHPR
                     DLCDQIIDISRYFSIEPVMSREMIDQAASSYFVEL"
     assembly_gap    15937..16036
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16037..16515)
                     /locus_tag="PLW25_03130"
     CDS             complement(<16037..16515)
                     /locus_tag="PLW25_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOS53514.1"
                     /translation="MEKTPSRIKAILLNFLVVIIWASSWVLIKIGLKDIPALTFAGLR
                     YFLAFLFLLPFIFQKKVRQQIRDFNKSDWRLIILMGVFSYFVAQGTQFLSLDYLPATT
                     LSLLLNLTSILVTISAIFLIKELPTKVQVLGVLINLAGMFVFFYPDGFSGNSTLGFV"
     gene            complement(16517..18229)
                     /gene="recJ"
                     /locus_tag="PLW25_03135"
     CDS             complement(16517..18229)
                     /gene="recJ"
                     /locus_tag="PLW25_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOS53515.1"
                     /translation="MAERNLTKRWEIAPKIDGMADEALADYPPFFRQLLYNRGIRTAQ
                     GAQQYLSNDGELADPFLLLGMESAVDRLVAAIDNGEKIVVYGDYDVDGVTATVMMVEV
                     LQRLGGNVEPYIPNRFEEGYGLNDEAIQTLASEHSAKVILTVDCGIRSPREAITASNL
                     GIDLIISDHHFPKGDLPAGYAVLCAKQERDPYPYKDLAGVGVAYKIAQALFIKKGMDV
                     GKADEWLDLVALGTVADMLPLTGENRIMVARGLNHLRNGSRVGLNALIGVSGRNVQQV
                     TATDIGFILGPRLNAAGRMESALQAYELLVSKSAVDAVKAAQVLDNQNSERQKATLAA
                     QEKAQGAVTHLESTYLITAFDSEFSSGIVGLVASKLVEKFHRPAIVGERLGDTIRASC
                     RSINGFHITKALDECADLLVRHGGHELAAGFTVKMENEDQLIARLQEIAARELSAQDL
                     RPTLRADMEIELSRFKPEYYRMIDQLQPTGMGNPAPLFISRGVVVDRMQVMGKESTHL
                     SFSVRGSQINRAVAFNQAAWYQTWYEEKPRFDLAYTVEVNHYNGMDTLQLNIRDMKIS
                     AVSF"
     gene            18388..20184
                     /locus_tag="PLW25_03140"
     CDS             18388..20184
                     /locus_tag="PLW25_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOS53516.1"
                     /translation="MTQDQTFTLIILLAAVILFASNRVRADMIALIVMVSLGLTGVTS
                     ADEAFSGFSSSAVMTILGISIISIALQITGATNSLGNILHTAGKGNEKLVILLVTLFG
                     ALVSLFMNNIAAVGVLLPAVMTLSRRSKISPSRLLLPLAYGTILGGMATLLTTSNIIV
                     SGALKDAGLPYFGLLDYFPIGGPVMLMGILYLSFIGFRLLPAKSPTNKIEDRREFTEK
                     LSDFYQVGKNLVKIELLPQSEMAGKTIANGHWYNRVHVNILAVLREKENIFSPDPCTM
                     LKPGDILIARGKVTEQSAKDLNLRVSAINNQEIKITDEAHPLAEVIISPHSRFIGKSL
                     QQMDFRERYQLNVVGVWRGGKPVEGEYADLSLHFGDALLVQGPSIQIRNLKKSEDLVV
                     LEEDPDAVLLPRKGLLTIFITLLTLTIAGLGFLPVALVVMGGAVLLILTGCVGLNDAF
                     RSIEWKAILLIAGLWPMSIALQSTGLAEMAVNSLLRLSGGLPPLQLAALFLFVAALLT
                     QLMSGQVTAIVMIPLALVSAQRMGIDPRPFAMAVAMGCSLAFISPLGHPVNIIVMNPG
                     GYTLKDYVKVGLPLTIAAFLCVIFAIKLYWGL"
     gene            20194..21315
                     /gene="tgt"
                     /locus_tag="PLW25_03145"
     CDS             20194..21315
                     /gene="tgt"
                     /locus_tag="PLW25_03145"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOS53517.1"
                     /translation="MEQIDFSYTLLKEQGSARAGIFFTPHGPIETPVFAPVGTQATVK
                     AVTPAQLEQTGATLVLANTYHLYLRPGAELVAAHGGLHSFMQWQKPLLTDSGGFQVFS
                     LSGSRKVDEDGVTFKSHIDGSMHRFTPESSIQIQELLGADIIMAFDECAVPDDLLYSR
                     VALERTHAWLERCIRQKKRPDQALFGIVQGGVFAELRRESARFVSSFDLPGVAIGGLS
                     VGETKAQMHAALEEVTPLLPRTKPRYLMGVGSPEDLVNGILRGVDIFDCVLPTRLARH
                     QAAFTPNGRVNLMNRMFAHDFNPIDESCHCYTCQHFSRAYLRHLIVAKEMLSATLISI
                     HNIQFLVDLVKRARKAILEGSFPLFASSFLEAYQIKEPS"
     gene            21312..22892
                     /gene="uppP"
                     /locus_tag="PLW25_03150"
     CDS             21312..22892
                     /gene="uppP"
                     /locus_tag="PLW25_03150"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HOS53518.1"
                     /translation="MSLIQSLILGIIQGFTEFLPISSSAHLVLVPFLFNWAIPENQVF
                     PFDVLVQLGTLAAVILYFWKDLWKIIKAFVTGLIRRQPFAEEDARMGWYLLLATLPAG
                     FAGIFLKERVEAAFNNPRLTAIFLFVTAVFLVGAEFFGRRSRQLDKMTWKDALWVGVF
                     QAFSIFPGVSRSGSTIAGGMTRNFKRPAAARFAFLMSIPIMLAAGIFSLPDLFAVPDL
                     PAFLPIVLVGFIAAGVVGYLSIRWLLSFLVKRSLIIFAVYCILLGSAVLIISGIRQTS
                     PNVAGTAPGAPAAESAEYPPQTPISIQETLPLEISPALDWLRPRISGCADAIPGLALV
                     TEGTVPANGEPMQLSWGEPPNLKRKAVIIGEDDLVFIVNPRNPLRELPLALLQPLLSG
                     ELNSWGEVQVACPECFSAGQLDELIDQPVEALLYPANVDARRVMDEALSSAPVRPNAS
                     SVLVPSAEMMKQEVSSALNAFGTLPSRAVDETVKVIPIQNGAETLKITRPLLATSAAE
                     AQPLTLQFLTCLQAAITP"
     gene            complement(22971..24308)
                     /gene="glnA"
                     /locus_tag="PLW25_03155"
     CDS             complement(22971..24308)
                     /gene="glnA"
                     /locus_tag="PLW25_03155"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOS53519.1"
                     /translation="MDKKELLERVAEDRVRFISFQFSDVSGTVKSVDAPISRLEGAVN
                     QGIWFDGSSVEGFARIQESDMRLKPDIDTYAVLPWSPENMRRARLFCNIYNPDGTPFA
                     GDPRGNLSRQLAALEERGMRLMVGSEPEFFLFRRNGSSDIHPVPHDVGGYFDFSPNDE
                     AVRVRTELMQALDSMGLEVEVGHHECALGQHEIDFHFSNALRSADNVLTLKYTVKAIA
                     AQNGLIASFMPKPIFGINGSGMHCHQSLFDLRGNNLFYDAADEYHLSPLAYGFIAGQL
                     AHARAFSAVTAPTVNSYKRLVPGYEAPVYVGWAQTNRSALIRIPRPLEGMTTSVRAEL
                     RCPDPSCNPYLAYSSMLAAALDGMKQQLTPPEPLNDVNVYELSREERQNLNIVELPGS
                     LREALVELEKDSVIQQALGETLYNAFRRAKWAEWERYRLDVTDWELARYLETA"
BASE COUNT         5886 a         6885 c         6000 g         5973 t
ORIGIN      
        1 gttgagagtt gagagattta cttatccaac agggcagcca cacccggcag ttccagccct
       61 tccagcatct ccagcgatgc tccacctccg gtggagatgt gcgtgatctt gtctgccaac
      121 ccagattgtt ggatggcagc cactgattct cccccgccta tgacgctcac cgcagcgcta
      181 tccgcaactg ccttcgccac tccaaaggta ccttccgcga acttcggaaa ttcaaaaacg
      241 cccatcgggc cgttccaaac gacagttcgc gctgtggcaa tgatagcccg aaaagcctta
      301 accgtctcgg ggccaatatc cagtatgcgc cagccatccg gaacatcctg gactttcaac
      361 gttttgcttt cagcatccgc ggcaaattga ttcgccacca ccatgtctac cggtaggtga
      421 atttttccgc cggcctcttc gaataaatca cgggcaattc ctaacgagtc atcttccacc
      481 agcgaatcgg ccacagccaa cccctgcgct ttcataaaag tattcgccat tcctccacca
      541 ataagaatgg cgtctgccct ggtcagcaga ttcttgatga cgccaatctt atcgctgatc
      601 ttcgcgccgc ccatgatcgc cacaaagggc cgctgcgggt tggccatcac ctgaccgaga
      661 tatttaattt ccttctccag taagaatcca gccgccgctg gcaagaaatg cgtgactccc
      721 tcagtagaag catgcgcacg gtgcgctgtg ccgaatgcat cgttcacgta aaggtccgcc
      781 aggctggcca attgcctggc catttcaaca tcattcttct cttcttccgc gtggaagcgt
      841 gtgttctcca gcatcaacac ctccccgggt ttcagttctt cggcagcctc cttggcctcc
      901 gggccaatgc aatctgagga aaactttact ggtttcccta ttaacgaaga aagatgctct
      961 gccacgggtc ttaatgagta cttcgggtcc ggccctcctt tgggtcgtcc caggtgcgag
     1021 cacaaaatca cagccgctcc cccatctaaa aggtactgga tagtgggcag cgcggctctg
     1081 atgcgggtat catcaccaac accagtctca ttaagaggaa cattaaaatc cactcttacc
     1141 agaactttct tcccttttat gtcaatatcc cgaattgtct ttttttcaaa catcgttcat
     1201 tctcctggtt aaggtaagat gcgcaatccg ttggtttttc ggttgcgcat cgtattcatc
     1261 aagcttaccc gggaaacatc accggatgac ttcttctata acttttttcc caacatcgct
     1321 gccaggtcag cggtacggca ggagtagccc cattcgttat cgtaccaggc gaccaccttg
     1381 accaaattac cgcccatgac catgttcgta ggcaggtcca cgatcgaaga gcgcggatcc
     1441 cctttgaaat ccatcgacac gagagggtct ccatactcgc cagtggtgta cccgaggatt
     1501 cctttcaaag aaccgttggc tgcttcaatg aaagcagcgt tcagatcatc tttcgtgatc
     1561 gttttttcaa tggtcgcaac aaaatcaaca atggaaacgg tcggcgtcgg aacgcgcagc
     1621 gagaagccat caaattttcc tttgagctcc ggaatcacca gtgccaaagc cttggcagcg
     1681 cctgtggtgg tgggaatgat attcaagcca gccgcgcgag agcgccgcag ttctttttta
     1741 tgtccttgat caagaatgac ctggtcattg gtgtaagaat ggattgtagt catcatgcca
     1801 taaaggatgc cgaacttttc gtgaaccacc ttcgcggctg gcgccaggca gttggtggtg
     1861 caagacgcat tggagatcac gtggtgcttc gcggggtcat accagccatc attcacacca
     1921 agtaccaatg tgaggtcttc attcttggcg ggtgcggtga taatcacctt cttcgcaccg
     1981 cctttctcga tgtggaccac tgcgccaggt ttgtccttgg ctgggtcgcc cttggcgtcg
     2041 gtaaacacgc ccgtagattc cacgacaata tccactccca ggtctttcca aggcaacatt
     2101 ccagggtctt tttccgcaaa aacctgaatc ctctttccat ccacgattaa ttcgttttct
     2161 ttcgcctcga tatgcacagg caggataccg taatttgaat cgtatttgaa taaatgggcg
     2221 tttgtttctg tatcaaacaa atcattgacg gccaccactt ccacttcccc ggggtggtac
     2281 tcattgatcg ctttgagaac ctgacggccg atacggccaa aaccattaat accaactttg
     2341 gttgtcatct acttcctcca aaatttaaaa tatcgatgaa ttttaccatg tccagacaag
     2401 attaaataat taaatggatg atttttcttc tttagagaca agcggcccgg tacgttccat
     2461 atagagatca atgatggttt tcgccacttt cgaagggtcg tgccgccacg gtttttcgac
     2521 attcacaaag tcagctcggt agtaaggata gcggttgtca tcctcacaaa ccttaaccca
     2581 ctccgtccct tctggcatat gatggtcata ccggttgtta gcaagcacta cgtcaaatag
     2641 cctgtgaccc gcgtgttttt caatcgctcg cacgtgatcc tcgcaggtat acccgtctgt
     2701 ttcacctcgc tgtgtggcga tattgcaaat atagaacttg taggctttgc tcgctttgaa
     2761 tgcttccaga aggtcaggaa ccaacagatt ggcgataata ctggtgtaaa ggctcccggg
     2821 gccaaccagg atgaggtcag cgtttagcaa cgcctgaatt gtcggcgggt aggatgtcgg
     2881 gttgcctgga tccagccaaa cccggcgcac ccgcccgcca gcctggctga tctcgctttc
     2941 tcccctcacc tggataatgg ttttattttc cgccagttca acttcaccaa caagattgat
     3001 atttttaaga gttgccggca agacacttcc ctgcactgcc agcacccggg cggattccga
     3061 gatggccgct tcaaaacttc cagtgatttc tgttaatgcc gtaatcagca gattgcccaa
     3121 tgaatgaccg tttaagcccg ctccggaggc aaatcgatat tgaaaaagct gggtcatcat
     3181 ctcttcgtcg ttagataagg ccgcaaggca attacgcaag tctccgggag gtaaaatacc
     3241 gatggattta cgaatttctc ctgacgaacc gccatcatcc gccacggtaa caatcgcggt
     3301 gatgttgctg gagtagcttt tcaatcccct caaaatggac gtcaaccccg tgccgccgcc
     3361 aatgaccaca attctcgggc ctttttcttt gcgtctgaac tcagctacag tgtcgatgac
     3421 atccttgccg gggcgtaaga agggccgcaa cagggcgcgg tttaatcccg ttataccaat
     3481 gccgataagc accagaccaa gcccgccaaa aatgaacgcg cgcaggatac ggttgggcat
     3541 gaattgtagt gcaacaatct tgatgaatgg cagccaccaa ccctcaggcg ctgtgcggta
     3601 aacatccagc aggtacaccg ccaagcccaa ccccagcaat gtcgtgccgg cgagaatcac
     3661 cagtacccag cgcttgatgc caatacctgg gaccagccaa cgcaactgca ttagcacaga
     3721 ttgaataaat cgaccaaatt tcttcgagcg tcgtctacgc attatgctag ataatagcag
     3781 ggtttaagag gtgcgtcaat aaatttcttg gcagcggttg tgagagacaa aaggctgttt
     3841 ttccacttac catggttgcc accatgaacg ataacctaga taaaaactgc aatttgagcg
     3901 ctcatgttat aatctcttta aggtcgaatg caaggcgcaa taaatccgca gtagtacgaa
     3961 aaaaattcaa taaccccagg agggcggtgt gaaccccaca agattacccg gccagaaagc
     4021 actcgagatt attcagcgcg accagcaggt gatatctcct tcttacccgc gtggttaccc
     4081 gttcgtgatg gaccacggga aaggcgttta tgtctgggat gtggatggca atcggtttct
     4141 ggattttgca gccggtattg cggtcaattc tactggccac agccacccgg acgtggtaaa
     4201 agccatccag gaccaggctg aaaaattcat ccacatttct tcggactttt atcacgctaa
     4261 gtgggtggaa ctggcagagt atctcaatcg tattgcccct tttaaagaag acgccgttat
     4321 tttccagacc aattcaggca cagaaagcgt cgagtcagcc ttaaaactgg cgcgttatca
     4381 cacgggacgc agccaattca tcgccttcat gggagggttt catggacgca catatggcgc
     4441 ggtcacgctg acagccagta aagccaaata ccaccaaggc tttttcccgc tcatgaatgg
     4501 cgtcacgcat gtgccattcc ctgatcccta ccgaccgatg cttcatatgc tgccgggtga
     4561 agatatcggc gatgcgacca tccgttttat tgagaaggtt ttcattcata acctccctcc
     4621 tcaggacgtg gcggggataa tcattgaacc catacagggc gaaggcggtt acgttgttcc
     4681 gccagacact ttcatccctc agttacgtga actgtgcagc cggcatggca tcctcctcat
     4741 tgcggatgaa gttcaatcag gtgttgggcg taccggtcga tggtgggcga tagaaaactt
     4801 tggtgttgaa ccagacattg tcaccactgc caaaggaatc gcttctggca tgccgctggg
     4861 cgcgatgatc gcgcgcaaga gcattacaac ctggccaaaa ggcgcgcatg ggaataccta
     4921 cggcggaaat cccctggctt gtgtcgccag ccttgaaaca ctccaactga tcgaaaacgg
     4981 ctacatgcaa aacgccaaac acgtgggcag ctactgcctt gatcgacttg ctgaaatgca
     5041 ggtcaggcac ccatccatag gggctgtgcg cggcatgggc ctcatgatcg gcattgaatt
     5101 tgtgatggat cgtgaatctc gtgaaccggc cgagaaaatg cgcgacgccg tggtggacaa
     5161 agcctttgaa ctcggtctgc tcacgttggg ctgtggcacc agcaccatcc gcatctctcc
     5221 cccgctgtgc atcacgcgtg aagaaatcga tgaaggactt actatccttg acgaagcgat
     5281 caccttcgct gaaaagtaac accaaaacca atcggcttca ttcgaaagcc tgcagaaacc
     5341 gccatgttac ctgattttcg agtccgccag cgggattatc tgttagagat ttcaagagct
     5401 ctcacccagg agctggatct ggtcaagctc ctggatcgca tattgcatat ttcattggag
     5461 atgctcgccg gtcaggcggg gctcatcgcg ctgcgtgttc cttcagaagg atggcagata
     5521 caggtctcca ccggccttcc gcccgcattt ttaaggtttc ttgaacctca actggctcaa
     5581 atttccgagg aacatgattc atccgatttg gaattaaaga ccatcaacca catccttggc
     5641 aacttcaccc agtccatcag cctgggtcga ttgagcagcg tgggtctgcc actgatcacg
     5701 ggtcaaaaag tgctgggggc gatctttgtt tttcgtgaat ttgcggcgtt ttcagccaat
     5761 gaccgggcgc tgctggccag ctttgcagat caggcagcca ttgccgtgca gaatgcgcag
     5821 ttatacaccc aggttgagaa tgaccgtcgc cgtgttaccg ggttgttgga ctcggccgct
     5881 gatggtatcc tgattctcac cagagccctg cgggtggaaa gggtaaaccc ttctctggcg
     5941 cacctggttg gaatacagcc ccaggatttg accggtaaag agcatgatga agtgattcgt
     6001 tgggtcaaac cgccgcatgg aatgaccctg actcaagcca tcgcggatgg ttggccgctc
     6061 acccctcatg ccgcgctcta tgtggaaggc gatttagaaa gaacaggcgg tacgcgccct
     6121 gttccagtgg gcattaccta cgcccctctg ctttctgaag agggaaagct cgtgaatatc
     6181 atcaccaccg tccgggatat cacccgtttc cgtgaagcag atgaacttaa agcaactttc
     6241 atttcagtgg taagccatga gcttaaaacg cctgtcgcgc ttattaaagg atatgtcagc
     6301 acgctcaggc gcgatgatgc ccactgggaa aaagcggtga ttaatgacag cctgcagatt
     6361 attgaagaag aagcagatcg tttgagtgaa tacattgaaa acctgttgga tgtgacccgc
     6421 ttgcaggcaa acggattcaa ccttaagcgc aacgacaccc aactggctga aattatcaag
     6481 cgcgtggcag agcgcctgca aacccaaacc atcaagcaca ccattattgt ggagattcct
     6541 cagcccctgc ccatcatcgt tgcagatgat tcacgcatca accaggtcat ctccaacctg
     6601 gtggcaaacg caatcaaata tgcccctggg ggtgagattc gcattaaagc agtccagcgc
     6661 gaatccaata tcatcatctg cgtcagcgat gaagggccgg gcatcaaccc caatgatgtg
     6721 ccgtttattt ttgaccgctt ttaccgtggt tcagatgcca gccgtctaac gaaaggcgca
     6781 gggttaggtt tgtttttagc caaatcgata gtcgaagcgc acgggggaag aatttggatc
     6841 gattcaaaaa ttggtcaggg tacgcgcatc tgtttttgcc tgcctattgc cagcaccggg
     6901 taaccagcac aaaacaaccg tttttcatca aaaaccgcag tggtataatc accctgttaa
     6961 attgatcttt ttgaatgaaa gagtggaact atgccaaaaa ttcaaaccgc atgccctcgc
     7021 tgtaaacagc caatcaccgc tgaggttcaa atgctcttcg atatgaacac agaccctacc
     7081 gcgaagcaaa aactacttgg cagagccaat aacttcgctc actgccaggc ttgtggttac
     7141 gaaggtctga tcagcacgcc aatcgtctat catgatccca ataaagagtt gctgctcact
     7201 tattttcctg gtgagctcgg gtttcccttg aatgaacagg agaagcagat tggacctttg
     7261 gtcaaccagg tagtaaatgc gctgccagcc gaaaaacgca aaggctattt atttcaacct
     7321 aattcgatgt ttacatacca gtcgctgatt gatcgcattc tggcggcaga cggcattacc
     7381 aaagaaatga tcgaagccca gcagaaacgt gttaacctga ttgaacggtt gttgagtttg
     7441 cccaatgaag aagaccgcgt taccgtggtc agacaggaag ccaacctcgt ggacggcgag
     7501 actttcacac tgctttcaac gctgatccaa tctgcctctg cgcagggcga tgaagccagc
     7561 gccaaacttc tcagccagat tcaggcagtg ctgctgaacg aaaccgattt cggaaagaaa
     7621 ctgctcgctg acagccagga aactcaacac gccctcaagg aactggataa agcgggcaaa
     7681 gaagggctca cgcgcgaaaa acttttagag gtgttgtcgt ccttaaaatc tgaaaccagc
     7741 ctgacaacat tggtgagcct tacccgctcc ggcttggatt accagttctt ccaaatcctg
     7801 agtgaaaaaa tcgaacagtc aacagatgaa aataagcagg cgctcatctc actcagagac
     7861 aagcttctcg accttaccag gaagatcgac aatgaagtta aaaagaggct tgaaaatgcc
     7921 gcttccttgc ttgaaaacat cctgaaagaa gcagatgtcc aggcagccat tgaaaaacac
     7981 gcggaagaaa ttgacgagtt ctttacgcag gctgtccagg ttgagtttga aaaagcgcac
     8041 agcgaaaaca acctcgaaag aatggaaaaa atccagaaag tcgtctcgat attggataag
     8101 atgaccgccc cgccgccaga agtagtgctc attcagaaac tgttagatgc cagtgatgac
     8161 gaaacccgca aaaaaatctt gcaagaaaat gaaactttgg tgaatgacca gcttcttcaa
     8221 accatcaacg ccatcatcac cgaaagctca accaaaagcc aagcgccgga acttatcgat
     8281 acattaaaaa gcatctataa gatggcttta cgtgcccata tggaaaagaa cctgaaatcc
     8341 tgatttttcc aaccttaaca aacaactccc cgaggctacc ccgtggctgc cttggggagt
     8401 tgttttacca gtgctgaatg gtcaagcagg agtcttcagg gattctccag cctgaggcca
     8461 tgaccgcgta ccgtgacgat atattgatgt ttttggtcga gttctgccag ccgctcgcgt
     8521 aagcgccgga tgagcgcatc aagcgcctgg tcggacacac cggtcgcttc atcctcttcc
     8581 caggtttttc gaatcaacgc ttctcgtgta accacctcgc ccgcctggtt atacaacgtc
     8641 gcgagaagac ggaattgggc agcggataaa ggaggcagca attcttctct gccaatccaa
     8701 acgcgccttg agcgttcctc aagcacgagt ttcccgctac cctttagaaa ggtggggtcc
     8761 aaatcctcaa tgggcattgt ggcgtctgaa ctgaagaagg cgaatttctg gatcaaggcg
     8821 atttgaaagg tatctccgtc acccaggcgt gttgattcaa tgaggggtgc acctgaaaga
     8881 aaagtgccat ttttacttcc gaggtcctca atataacaga taccgttttc gtttcgcaac
     8941 cgggcgtgat ggcgggaaac ctgtcgctct tcaatacgga tggcgcaatc ttcatcacga
     9001 cctatatcta tcgcctcacg gatgatccac cgcctgccct caagtggccc ctgcagtgct
     9061 actaaaacgg gaaattcatg agtgatagag ttgccgaatg aagaatttct catgcattcc
     9121 tcaaacatga ttatacaaga ttgtcctcag gtataatagc aatatgggta accaatcctt
     9181 ctctttcatt aattcggtaa gttcataaat gatatcaccc ctaaaaaaag gcaacatcct
     9241 gcgtgaaagg tatactatcc gccgcgttat cggtcagggt ggcatgggtt gtatctatct
     9301 ggcggatgac ctcaggctgg agggccggca gtgcgcggtt aaggaagtcg aacacgatcg
     9361 aacacttccc aaaaacctgt taaaagaagc tcgcgaccaa tttcaacgag aagccacagt
     9421 attggcgcgt cttgaccatc ccaacctgcc aaaagtttcc gattacttct caattgagaa
     9481 cagtgattat ctcgtgatgg attttgtacc cggagaggat ctacgcacgc tgatgctaaa
     9541 acagcgtgaa aaaaatcagt tcatacctga aaaagacgtg ctcaactggg catttcagct
     9601 ggcagacgcg ctcgaataca tgcacaatca aaacccgcca atcctgcacc gtgatataaa
     9661 acccagcaac cttaaagtga ctccagcagg gcttctcaaa ctggttgatt tcggcctggt
     9721 gaaagtgctg gcccccggcg aagtcaccat taccatcctt caaggtcaag gaacggcact
     9781 ttataccccg ttggagcagt acggcggtga tattggtcat accgacgcgc gcagtgatat
     9841 ctacgctttt gctgcaacgt tgtaccattt gctcactgcc aaacctccag tagatgcccg
     9901 ccagagattc atcagcccaa acgctttaca gccaatccgt gaaaccaatc aggcggtcac
     9961 ccagaaaact gaaaatgcta ttttatgggg aatggccttg catccggatg atcgcccatc
    10021 caccatcggt caatttcgcg aagccctgat tggcagccgc cctgtcgcga ttccgtcagg
    10081 aaacaggttg tcagtgaaaa ttcccacctt ctacaaccgt aagattggga aacaatggta
    10141 cattctgatg ggcttgagcc tattgaccct gctggtgaca ctactgcgct gagtgctatt
    10201 tgcgcgtttt ccaccagtaa aacaaaaaac cactcccaaa tccgcagacc aacccgatac
    10261 ctacgagaat taatatttgc ccagatgcaa cctcgttgag cggtgatttt gagccaggta
    10321 agcccagtgc agcccaaagg taccccgcat accctcccgc accagaaaac aagatggtga
    10381 gaaaattcca ttttgcgctg aggtgattgt gggtaaaata actcaagcct aggcaaaaac
    10441 caacgatcaa gatattcgcc agtaaccaat cccaaaaagc cacttttccc tgtttgtgca
    10501 attgtataac cttgctctct ggtgtaggtg agggcgaagc ttccgtcggg gtggctatat
    10561 ctgaacttac tggaattgga gttggctcaa aagaagtaac gactcctccg gtgtctgtaa
    10621 tatcaagcct caggatctga gagatgaatg cagggtctgc agtcacgctt aattgcaggc
    10681 tgccaatact gggaatcaca tacatcacac gagcaaagcc atcgatcgtc ctggcttcaa
    10741 gctgttcaac agagccactg gttgaccggg tatcaataat gaagcgcacc agggtcccat
    10801 ctggcacagg attgccattg tgatcaacaa tcacgccagc cttgatgggt atcgtatctc
    10861 ccgccttata gagtaacggt tggctgtaac ccgccaaagg tggtgcctgc ggttcatcta
    10921 tttcgacggg cgtctcgatc accaaaggga tcatttgggt tgggtctggt gagagcacgt
    10981 aaaccaggtc atatccgatt ccgggcacag acacgggcag cttgcctgag ggcgtcagct
    11041 cctgcatcac cacacgcgaa gcgatctcat aaaactccgg cgtattgcta taaagagcgt
    11101 aataagccgt taattttgaa atgtcagtgg agtcaaggaa gtagggcgcg ttaaaggcca
    11161 gcccaatcag ctttttattt cttaccagat caggtctctc gtcgaacaag cgtttatagg
    11221 tggttgcttc tactctttca gcgccaaatt cagcgaatga aaagatgatc catgttgcgg
    11281 cgtttaaaga gaccagtata tcttctgtcc tatctgcctg ttccagtacc atcctgaggt
    11341 tctcatagct gtaggcggtg aggtgactcg ccggaatggg atcacctgcg cccgacccgt
    11401 aaagccggat gattgaattc atcaatcggg cagcaggaaa atcagtttca accgggcaac
    11461 tgctgcattg cgtgttggtc tgcacatttg agataaacac aatgcggtca tttgctcctg
    11521 gggggtccgg aagaacagta ttaagatcct ccatctcagg gctgatcagc gtaacggcag
    11581 attgcgcgat ttccatcacg gttgcttggg cttttcctac agtctccagc tggttgcggg
    11641 gtgtcatcac atcagccgga tcaaatgaag ggtacaattt catcttcaat tgcaagattt
    11701 ttagaactgc ctcatcaaca cgattgtgaa acgcgctgtc ttcttcatac ttttgaacaa
    11761 aatagtcaat ggttcttacc accgttgagt aattatcttc atctcctgtt gccaggatgt
    11821 ttcccatata gagcaggtcg tttccagcca tgagcgcgct gcgagcaacc tgtcttgcat
    11881 caaacccggt gttcaaaggg tctacaaatt tacgaataga ctcgctcccc aaatcgtcac
    11941 tgacgatcaa cccgcccatt ttcctccagg ggcttagttc tggcaggttc atgatttgtt
    12001 ccacagctac tgggtcaaat gagatgggtt tggttgaaga gcgaatattg ccctggaacc
    12061 cctggtaacg aatgtgagaa agcagcaacc catccaccac cccgatgtga ccttgatcga
    12121 tttttgttac attgaaaaaa ggcgctaatt ctacacgcat caattggtca agtgattttc
    12181 tgaccgtggc gatttcttca tccggttgcc tgtctgagct gccgctgccc ggaaagtgtg
    12241 tggcgatcac agccacacgg ttcgcgctgc cagcttgaac cccctcaatg taggcttttc
    12301 ccagcttacc cacccagtaa gggtttccgc caaatgtttt tacgcccagg tcgtctctgc
    12361 cctcggtatg gatgatatcc agcacgtcca atgaaggccc aatcaacata ttgatcccca
    12421 acgcttcgag ttctgagccc aataccgacc caactgcgct ggcctgttca gttttccaac
    12481 tggcaccaat tgtcatcaag ttgggaaggt tggtaacgcc atgcaggatt tgtccatcta
    12541 catccaggta tccattttgc gtgatcgcaa ccaacagcgg cgcgtaagac tcctggtttt
    12601 ccaccatctg agcggccgct tgcaggtctg taaccagttc ttttaaaacc ccaattgtgt
    12661 tttccggacc gataaaatta tcattatcct ttctcagtac cactccgcca atatgctttt
    12721 cttgaataag tctgactatc cggctgccct cctgcgctga ggacccctgg aaagagatga
    12781 gaaaaacctg ccctactttt tcctcagtgg tcatttgcgc tagccgaacc tgcgcagcgg
    12841 ccaggcgcgc attcgtttgc gcgcggtctc ccgcctgggc ggttgcggta ggcgctgcca
    12901 gacttagggc aagaaacagc aggaaaattt tttgcaaggt tttcttcaag cgatttagcc
    12961 agccttactt tgaatgaata atttcattaa atatttcggt ttgaatattg ccgccggtga
    13021 ggatcagcgc tgcaggaagc aactgaattt taccagcaag tcgcgcagcc aacccaactg
    13081 ctgctgaacc ttctattctt tctccatatt tcttccagca aaaccggatg gctgaacgga
    13141 tttcttcctc gcttaccagt acgatatcat caacaaatcg acgcatcaat gggatggtga
    13201 ttgaatcgtg ggctatctcc cctgccagcc cttctgcgat actttctgtt tcggttacac
    13261 cagtttgcgt gcctgaataa taaacctggt gagcataagg agaggtttct gactgcacgc
    13321 caattaattg gggtcgctcc ttcatattgc ttaaatacac accaatgcct gagatcagtc
    13381 caccccctcc aaccggaaca accagtgagc ggatgccatc caatcccccc aattgttcta
    13441 ccagttctgg ccctattgtc ccctgccccg caatcacctg cgcgtcgtta taaggagaaa
    13501 tgaatgtttt cccgctagtt tgagcatgag ccaaggcctg tttttcagcc tcaacatacc
    13561 cacctctcac caggcgaaca tcagcgccaa actggcgcat cgcgtcaatc ttactcgcgc
    13621 tggtgttttc aggcacaaat accatgcagg cgctcccccg gctggcagca gcaaaggcaa
    13681 ctccttgccc atggttccct gccgaacagg cgaccaaccc ccctttcaac gcactctttt
    13741 ccaaactcaa tactttattc gaggctcctc tcaatttaaa agagcccgtc ttttgataat
    13801 tctcccattt acatagaatg tctaattgtg ggactttggt aacaggggtc tgctcgatga
    13861 ttggctggat gcgcttcgct gcgtcgatca cccattcagc cgttatcact ttcgctccaa
    13921 aatcctcaac gcctttgcag ggtcatcagt aaagaaacca tccaccccat agtcgatcat
    13981 ccttcgcaaa tcattatcct cgttgaccgt ccaaacgtgc acccttcttc ctcgtcttct
    14041 ttctgtattc atcaactgcg gagtaacatc atccaggtag gggtggatga tttttggaga
    14101 caactggatg aataaggcgg aacgagaagc aacgccaagt agaccctgcg aagccagcag
    14161 cgcaaccgga acatctggga gcagtatctt catcttcttc aggtttccag gaatgaagga
    14221 ggagaataat acttctttct ctaactggtg tcgcttaacg atctcagcca cgatcggcac
    14281 caggttatca tgacgggaag cgtaattcgt caattccacg ttgataaaaa tgcgtttacc
    14341 gacactctca aatacttcat ccagtgtggg tatttgttcc cctgcgtaag catcaaagcg
    14401 gtattgcgcg ttcaatcctt tcagagaggc taaggtcatc tggtttaccc gtccagaacc
    14461 atctgtggta cggtcaacag tttgatcgtg gatgaccacc gccttcccat cagcggaaag
    14521 ctttacgtcc aactcgatcg catccgcccc ctggcttacc gccagttcaa aggctgcgag
    14581 ggtattttcc ggggcgtatt gactggcacc gcggtgagca aaaacaacag gctttggcaa
    14641 atcgtcgagc atttacaact ccacaaaata ggatgaggct gcttgatcga tcatttcacg
    14701 cgacatgacc ggttcaatag agaaatagcg tgaaatatca atgatctgat cgcaaagatc
    14761 tcgagggtgc gaggctcgca gtttgcggtt gtgtttggca taccattcct tcaagaggta
    14821 gttcaatccc tcgtcagaaa acacaacccc tttctgcgcg gccacccgca tgaatatctc
    14881 gcggtaatgc actgcatcgg ggtctgtgat ctcaattttg tgccgcagtc gccgcaagaa
    14941 cgcttcatcc actaaatcct ttggcggcag gttggtggaa aacatcacca gcacatcaaa
    15001 aggcacttcg atctttcgcc ccgtgtgtaa tgaaaggtag tcaatgcgat tttcaagagg
    15061 aacaatccag cggttcagca agtcgcgcgg gcgcacctgc tgccttccaa agtcatcaat
    15121 taagaggatt ccgccgttcg cttttacctg aaaaggagct tcgtaaaact tcagggtttc
    15181 gttaaaaacc aggtccagtc cggtcagcgt caattctccg cccaccataa taaagggtct
    15241 ttgaatcttc acccagcgct gatctttcct gcctttcatc cccctgtttt gcgggcctgt
    15301 ttcgtcttcg gcagccagct ggtggttcat agaatcgaac atggtaatga cctggccgtc
    15361 gacaaatata gcgtagggaa tatacattgt ttgcgtgagc acctggttgc caatcgcgcg
    15421 ggctacactg gttttcccgt tccctggtgc gccgtaaatg aaaatggaag tgccagaatt
    15481 aactgccggg cctaatcgat ggaatacctc gtcgccaaag accaggtcgc ccataatttg
    15541 acgcatatta cggctggtca caaacatacg gccgcggctt tgccggagca cggattcatt
    15601 ataggcctca agcggcacag gcgctggtcc ggcgtattgg cagcgatcga gcgcttcgcg
    15661 cgcgtgggta atacccgcgc cggtgatcgc atacaaataa gcgccttccc ctaaccccat
    15721 ctgagaagat ttgacctcta caagcttatc ccgtttgaga gcatccagca gttggtccac
    15781 catccctgta aaaggcaggc ataaagcttc tgccaccttg tagcctgtca tgaatccctg
    15841 gaaatacaaa attttcaaag ccaggtcctg aaggaacagc gcgctgagac cagtatcttc
    15901 tattgtatta atggggtttg gggagaaatg gctgctnnnn nnnnnnnnnn nnnnnnnnnn
    15961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnaaaa caaacccgag ggtggaattg cctgagaagc catcagggta
    16081 aaagaaaacg aacataccag ccaggttgat cagcacaccc agcacctgaa ccttcgttgg
    16141 aagctcctta atgagaaaaa tcgctgaaat cgtgaccaat atcgaggtca ggttgagtaa
    16201 caggctgagt gtggtcgccg gcaggtaatc aaggctcaag aattgagttc cctgagcaac
    16261 aaaatacgaa aagacaccca tcaggatgat cagccgccag tcactcttgt tgaaatcacg
    16321 gatctgttgt ctcacctttt tctggaagat gaaaggcagt aaaaatagaa aagccaaaaa
    16381 ataccttagc ccggcaaaag tgagcgcagg gatgtcttta aggccaattt tgatcagcac
    16441 ccaggaagaa gcccagatga tcaccaccag aaaattcaac agaatggctt tgattctgct
    16501 cggtgttttt tccatatcag aaactgaccg ctgaaatctt catatctcgg atgttaagct
    16561 gcaaagtgtc cataccgttg tagtggttaa cctcaacagt ataggcaaga tcaaagcgcg
    16621 gtttttcttc ataccaggtc tggtaccagg cggcctggtt aaacgccacc gcccgattga
    16681 tctggcttcc tcgaaccgaa aagctgaggt gcgtgctttc ctttcccatc acctgcatgc
    16741 ggtccaccac taccccgcgg gagataaaca atggcgcggg gtttcccatt ccggttggtt
    16801 gcaactggtc gatcatcctg tagtattcag gtttgaaccg gcttaactcg atttccatat
    16861 cagccctgag tgttgggcgt aaatcttgcg cgctcaattc gcgggccgcg atctcttgta
    16921 agcgcgcgat cagctggtcc tcgttctcca tcttcacggt gaaacccgct gctagctcat
    16981 gtccgccgtg ccgcacgagc aagtcagcgc attcatccaa tgctttggtg atatgaaagc
    17041 cattaatact tcgacaggaa gcacggatgg tatcccctaa ccgttctccc acaatggcgg
    17101 ggcggtgaaa tttttcaacc agcttcgatg ccaccaagcc aacgatccct gagctgaact
    17161 cggaatcaaa cgcggtgata aggtaagtcg attccaggtg ggtcacagcg ccctgcgctt
    17221 tttcttgtgc tgccagcgtg gctttttgtc tttctgaatt ctgattatcc aatacctgag
    17281 ccgctttgac cgcgtctacg gctgatttcg aaaccaacaa ctcataagcc tgcagggcag
    17341 attccatcct gccagcagcg ttcaatcttg gtcccaggat gaagccaata tccgtggcgg
    17401 tgacctgctg tacatttcgt cccgataccc cgatcaaggc gtttaaaccc actcggctgc
    17461 cattcctgag gtgattaagt cccctcgcga ccataatacg attttcaccg gttaacggga
    17521 gcatatcagc aaccgttccc agcgccacca ggtctaacca ctcatctgcc ttgccaacgt
    17581 ccatcccttt tttgataaat aaagcctggg cgattttgta ggccactccc accccagcca
    17641 ggtctttgta cgggtacgga tcacgttcct gtttggcaca aagaaccgcg taccccgcgg
    17701 gcagatctcc ttttggaaag tggtggtcgc tgatgatcaa atcgataccc aaattgctgg
    17761 cggtaatggc ttctcgcggt gagcggatac cacaatcaac ggttaaaata actttcgcgc
    17821 tatgctcact ggccagcgtt tgaatggcct cgtcattgag cccgtaacct tcttcaaacc
    17881 gattagggat gtagggttcc acgtttccgc ccaaccgctg cagcacctct accatcatca
    17941 cggtcgcagt gacaccatcc acatcgtaat ccccatagac aacaattttt tcaccgttgt
    18001 ctattgccgc gaccagtcga tcaactgctg actccattcc gagcaataaa aaaggatctg
    18061 ccaattcgcc atcattggaa agatactgct gggcgccctg agcggtacga atgccgcggt
    18121 tgtaaagcag ttgccgaaaa aatggcgggt aatctgcgag cgcttcatcc gccattccat
    18181 cgatttttgg agcaatttcc caccttttcg ttagatttct ttcagccatc gaaaagtcct
    18241 tttttatcta gcgatctctt ccatcaataa atgatcaatt aggccaggta aattccgctc
    18301 aagtataaac gagcagccaa tcacccgcaa ggcggttatc gtcttcagcc ccatcaagta
    18361 aaatagaagg atggatagga tgaaaaaatg acccaggacc aaaccttcac gcttattatc
    18421 ctgctcgccg cggtcattct gttcgcgtcg aaccgtgttc gcgcggacat gatcgctctc
    18481 atcgtgatgg tatcccttgg tctaacagga gtgaccagcg ctgatgaggc tttttctggc
    18541 ttcagcagtt ccgctgtgat gaccatcctt ggcatctcga tcatctccat cgccctacaa
    18601 atcaccgggg caaccaattc acttggcaat attctccaca cggctggtaa aggcaatgag
    18661 aaattggtca ttctcctcgt taccttgttt ggcgccctgg tatctctttt tatgaacaac
    18721 atcgccgctg tcggagtgct gctcccggca gtgatgaccc tttctcgccg cagtaagatc
    18781 tcgccctcac gcctgctgct gccgctggca tatggcacca ttttgggggg tatggcgacg
    18841 ctcttaacta cttcgaatat tatcgtcagc ggcgccctca aagacgcggg gctgccgtat
    18901 ttcgggctgc tggattattt tccgattggc gggcctgtaa tgctgatggg aattctttac
    18961 ctgtccttca tcggcttccg tttgctcccg gcgaaatctc caaccaataa aattgaggat
    19021 agacgtgagt tcacagagaa attaagcgat ttttaccagg tggggaagaa cctggtgaaa
    19081 attgaactcc tgccccagtc tgaaatggcc ggcaagacca tcgccaacgg gcactggtat
    19141 aaccgggtgc atgtgaatat tctcgcagta ctgagagaaa aagaaaatat cttttccccc
    19201 gacccctgca cgatgcttaa accaggcgat attttaattg cccgcggaaa agtcacagag
    19261 caatccgcaa aggacctgaa cctgcgggtt tccgcaatca ataaccagga aatcaagatc
    19321 acagatgaag cccacccact ggcagaggtc attatttctc ctcattcgcg ctttattggc
    19381 aaatcgctcc agcagatgga ctttcgcgag cgttatcagt taaatgtggt gggagtctgg
    19441 cgcggcggca aaccggtaga aggagagtat gctgacctga gcctccactt tggtgatgcg
    19501 cttctcgttc aaggcccttc cattcaaatc cgcaacctca agaaaagcga agaccttgtt
    19561 gttctcgagg aagatccgga cgcggtgctt cttccccgta aaggcctgtt gaccatcttc
    19621 atcactctcc tgaccctcac catcgctggg ctgggttttc tgccggtggc gctggttgta
    19681 atgggagggg ctgtgctgct catcctcact ggctgcgtcg ggttgaacga tgcgttccgc
    19741 agtattgaat ggaaagccat tctgcttatc gccggtttgt ggccaatgag catcgcgctt
    19801 cagtccaccg ggttggctga aatggccgta aacagccttc tacgcctaag cggcggctta
    19861 cctcccttgc agctggccgc actgttcctg ttcgtggctg cattgctcac acaactgatg
    19921 agcggacagg tcaccgccat cgtcatgatc ccgctggcgc tcgtttcagc tcagcgcatg
    19981 gggatcgacc cgcgaccttt cgccatggcg gttgctatgg gctgttcgct ggcttttatt
    20041 tctcctctgg gacatcccgt caacattatc gtgatgaatc caggcgggta cacgcttaaa
    20101 gactatgtga aggtgggtct tccgctcaca atcgcggctt ttttgtgcgt tattttcgcg
    20161 atcaagctct attgggggct ataatacaac tccatggaac aaatcgactt ctcttatacc
    20221 ctgctcaagg agcagggatc ggccagggct gggattttct tcaccccgca cgggccgatt
    20281 gagacacctg ttttcgcccc cgtaggcacg caggctacag tcaaagccgt tacccccgcg
    20341 cagctggagc aaaccggcgc cacactggta ctggccaata cctaccacct ttatctgcgc
    20401 cccggcgcgg agttggtggc agcccacggc ggtttgcact cgttcatgca atggcaaaaa
    20461 cccctgctca cagactccgg cgggttccag gtattttcac tctccggctc gcggaaggtg
    20521 gatgaggacg gcgtgacctt taaaagccac atcgacggtt ccatgcaccg gtttacgcct
    20581 gaaagctcca tacagattca agaactgctc ggcgcggata tcatcatggc tttcgacgag
    20641 tgcgccgtgc ctgacgacct gctttacagc cgcgtcgcgc tcgaacgaac ccacgcctgg
    20701 ctggagcgct gcatccgtca gaaaaaacgc cccgaccagg cgctgttcgg catcgtccag
    20761 ggtggtgttt tcgcggaact gcgccgcgaa tcagcccgct tcgtctcttc gtttgaccta
    20821 cccggcgtcg ccatcggcgg gctttccgtc ggcgaaacca aggcgcagat gcacgcggcg
    20881 ctcgaagagg tcacacctct cttgccgcga accaaacccc gctacctgat gggcgtcggc
    20941 tctcccgaag acctggtgaa cggcatcctg cgcggcgtgg atatcttcga ctgcgtcttg
    21001 cccacccggc tggcccgcca ccaggcggcg ttcacgccga acggacgcgt caacctgatg
    21061 aacaggatgt tcgcgcatga tttcaacccc atcgatgaga gctgccattg ttacacctgc
    21121 cagcactttt cacgcgccta cctgcgtcat ctgatcgtag ccaaagaaat gctctcagcc
    21181 accctcattt caattcacaa cattcagttc ctcgtagacc tggtgaagcg tgcccgcaag
    21241 gcgatcctgg agggcagttt cccgcttttc gcctcctcct tcctggaagc ctatcaaatc
    21301 aaggagcctt catgagcctt atccaatctc tcatcctggg aatcatccag ggtttcactg
    21361 agtttttacc catctcttca tccgcgcacc tggtgcttgt gcccttcctc ttcaactggg
    21421 ccatccctga gaaccaggtt ttcccctttg acgtgttggt tcagctgggt accctggcag
    21481 cggtgattct ctatttctgg aaagacctgt ggaagatcat caaagccttt gtgaccgggc
    21541 tcatccggcg ccagcctttc gcggaggaag acgcgcgcat gggctggtac ctgttgctcg
    21601 ccacgctccc ggctggcttc gcgggtattt tcctcaaaga gagggttgaa gccgccttca
    21661 acaatcccag gctgaccgct attttcctgt tcgtcactgc cgtcttcctg gtcggagctg
    21721 agttcttcgg gcgccgcagc cgccagctgg acaaaatgac ctggaaagac gcgttatggg
    21781 tgggcgtctt tcaggcgttc tccattttcc ccggcgtttc acgctccggt tcaaccattg
    21841 ccggcggaat gacgcgcaat ttcaaacgcc ctgcggcggc gcgtttcgcc ttcctcatgt
    21901 ccattccaat catgctcgct gcgggcatct tcagtctgcc agatctcttt gccgttcctg
    21961 atttgcccgc ctttctgccc atcgtgctgg ttgggtttat cgccgccggt gtggttgggt
    22021 acctttcaat ccgctggctg ctgtccttcc tggtcaaacg ctcgctgatt attttcgctg
    22081 tgtattgcat tttgttgggt tccgccgtac tcattatcag cggtattcgt caaaccagcc
    22141 ccaatgtggc tggcactgcg ccaggcgcgc ctgccgcgga aagcgcggag tatcctccgc
    22201 agacgccaat ttccatccaa gaaacactgc cgctggaaat ctcacccgcg ttggactggc
    22261 tgcgaccgcg catcagcggc tgcgctgacg ccatccccgg cctggcgctc gtgaccgaag
    22321 gcacggttcc agccaacggc gaacccatgc agctgagctg gggtgagccg ccaaacctga
    22381 aaaggaaagc agtgatcatc ggagaagacg acctggtttt catcgtcaac ccgcgcaacc
    22441 cgctgagaga gctgcccctc gcgctgctgc agcccctgct ttctggtgaa ctcaattcct
    22501 ggggagaagt tcaggttgcc tgccctgagt gtttttccgc cggtcaactg gatgagctca
    22561 tcgaccagcc tgtggaagcg ctgctctacc ctgccaatgt ggatgcgcgc cgcgtgatgg
    22621 acgaagccct ttcatccgcg cctgtgcgcc caaatgcctc ttccgtcctc gtgccttcag
    22681 cggagatgat gaagcaagaa gtctcctccg ccctcaacgc ctttggcacc ctaccctcgc
    22741 gggcggttga cgaaacggtc aaggtcatcc ccatccaaaa cggcgccgaa acgctcaaaa
    22801 ttacccgccc gctgctcgcc accagcgccg ccgaagcaca gcccctgacc cttcagtttc
    22861 tcacctgcct gcaggcggct attacgccct gagctcgcgc caccttcatc gctcgttcct
    22921 ctgcatcctc aggagataac ctggtcgcct ggtaaatgga tctgtcgcgc ttacgcggtt
    22981 tccaggtaac gcgccagttc ccaatcggtc acatccaggc ggtagcgctc ccattctgcc
    23041 catttcgcgc gtctgaaagc gttgtagagg gtctcaccca aagcctgctg gatgacggaa
    23101 tccttttcaa gttccaccaa cgcctcgcgc agcgaccccg gcagttccac aatgttcagg
    23161 ttctggcgtt cttcgcgtga tagttcgtac acattcacat cgttcagcgg ttcaggaggg
    23221 gtaagttgct gcttcatccc gtccagggct gccgccagca tgctggaata cgccaggtaa
    23281 gggttgcagg atggatcggg gcagcgtagc tccgcacgca ccgatgtggt catcccctcc
    23341 agcggacggg ggatgcggat gagcgcggaa cggttggttt gcgcccagcc cacgtacacc
    23401 ggcgcttcat accccggcac caggcgcttg tacgagttga ccgtgggcgc agtcaccgcc
    23461 gaaaaagctc gcgcgtgcgc cagctgcccg gcgatgaacc cataagccag cggcgagagg
    23521 tgatactcgt ccgctgcgtc gtagaacagg ttgttgcccc gcaggtcaaa gagcgactgg
    23581 tggcagtgca tccccgaacc gttaatgcca aaaatcggct tgggcatgaa ggaagcgatc
    23641 aagccgttct gcgcggcgat ggctttgacc gtgtatttga gcgtgagcac gttgtccgcg
    23701 gagcgcaggg cgttgctgaa gtgaaaatca atttcgtgct gccccagggc gcactcgtga
    23761 tgtcccactt ccacctccaa ccccatggag tccaacgcct gcatcaattc ggtacgcacg
    23821 cgcaccgcct catcgttggg cgaaaagtcg aagtaaccgc ccacatcatg cggcacaggg
    23881 tggatatcgc tgctgccgtt gcggcggaag aggaagaact cgggttcaga accgaccatt
    23941 aaacgcatgc cgcgctcttc aagcgccgcc agctgccggc tcaggttacc gcgagggtcg
    24001 ccagcgaacg gcgtgccatc cgggttgtag atgttgcaga aaaggcgcgc gcggcgcatg
    24061 ttctctggcg accagggcag caccgcgtag gtgtcaatat ctggtttcag gcgcatatca
    24121 ctttcctgga tgcgcgcgaa tccctcgacc gaagagccgt caaaccagat gccctgattg
    24181 accgcgccct ccagccgtga gatcggcgcg tctacgcttt ttaccgtacc actgacatcg
    24241 gaaaactgaa aggagatgaa ccgcacccgg tcttccgcca cccgctctaa aagttctttc
    24301 ttgtccattg ttgcgctcct atttaatgaa ttcaggcatg cgtgggggca tgcctttttt
    24361 cagacgcgct tacagcagcc cttccgctta aaaccacagg cacgccatcc tgcctggccg
    24421 gtatcagcta tctgtgccgc gcgtcgccgc gtggttttgt tcgctgactt gcatgggaag
    24481 ggagatggaa gcccctggca gggctccgga ggcatccgct gatcgttcga ttttccccta
    24541 tgcgatcccc ccagggttaa catccttctc gcgaaggaga acctcctcct cgtcatctcg
    24601 gcgaaccgag atgcagccgc tgtttctctc tttattcaat tgtgaatggc gcctatcata
    24661 cagaggtcgc gaggaaatgt caatacccgg gtttgctgct taagcaaaga gcaaggtcaa
    24721 cctccagcgg cgggtatggc gtggtgcgcg gagcctcctc atccgcgata ttcatcgcgc
    24781 accccgccgc ttgcctttct tgaacaaatg gtctataatc agcgtggcgc atccatacta
    24841 taat
//
LOCUS       DAOSPM010000020        24650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2043_length_24650_cov_16.035983, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000020 DAOSPM010000000
VERSION     DAOSPM010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24650
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2043_length_24650_cov_16.035983"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PLW25_03160"
     CDS             complement(<1..652)
                     /locus_tag="PLW25_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53520.1"
                     /translation="MGEPLLKVKGLETHFKTPEGTVHAVNGVSFHIKEGETLGFVGES
                     GCGKSVTMLSCLRLIPAPPGKIIAGEAFFKGNDLLKMTPDEIRHVRGGQISMVFQDPM
                     TSLNPVMSIGKQMAEPLMLHLNLTKEQARKRSIELLGLVGIPNAADRLKDYPHQYSGG
                     MRQRVMIAMALSCNPQILIADEPTTALDVTIQAQIVDLVKHLREELGMAIIWITHDL"
     gene            complement(777..1667)
                     /locus_tag="PLW25_03165"
     CDS             complement(777..1667)
                     /locus_tag="PLW25_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53521.1"
                     /translation="MKPAVSKRKQKAGLLYGLAAGLAFAIFTWGVDAFLLACAHGVFP
                     WVNFVPGLVIGLVSSALVGWLTARVERAWFSLLLWLLQALLFAHLVVWLPIKVAPRII
                     FIFNPALGDFLQYPFHHELYQNLWISFAVIALVSLIIGLIENILIEQALFSSGTFSLI
                     VPLVVCFLSFSLVGNFSDSILNKRFRESVQQVDEVIDFILAHEGQEVPQETALKMRLF
                     AFDHVKDVVSQKHGLIMSNYDEMLGQVDVLVDFNGQWVKCPVIYNQLVTCSRIFENPA
                     IRLGSTDKHMSTSVNEDPPI"
     gene            complement(1840..2751)
                     /gene="rsgA"
                     /locus_tag="PLW25_03170"
     CDS             complement(1840..2751)
                     /gene="rsgA"
                     /locus_tag="PLW25_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOS53522.1"
                     /translation="MSAETLKGIVTRFQSGFYNVQTAGGMIVCKLRGRLKRRSFQGDV
                     ISIGDNVAITRLPDGSGVIEAIEPRARELARLDPTPRGTYRQILLANPNQIVAVFACA
                     SPEPHLRMLDRFLVIAEKQGLGAVVVANKLDLVGKRNARRIFSIYPPLGYRVIYTSAV
                     TGEGVDELREALSGKVSALTGPSGVGKSSLLNRVQPGLELAVQEVSARKDRGRHTTVV
                     RELFPLDGGGYVADLPGLRKLSLWDTEPEELDGYFPELRELVKHCQFNDCAHYEEPGC
                     AVIKAVEAGKVSAERYESYLRMRVGDE"
     gene            complement(2754..4934)
                     /locus_tag="PLW25_03175"
     CDS             complement(2754..4934)
                     /locus_tag="PLW25_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOS53523.1"
                     /translation="MKINHFKFPWQILVTALLVLTACSNGAQPTIEPGGELPSPIVQT
                     TQLPDVSLTVLEYLEKWNQQDYSGMYAMLAPTSRDAISEADFVKTYTDVALNMTLREI
                     EYGILTTLVNPTSAKVGYQVSFKTSLFGDIIRRMEMGLLADHGKWWIQWESGLIMPEL
                     SGGKKLALSLTSPTRGDIYDNDGNIIAAEAEAVAIGVVPDEIADDQVGNLAGILTVMT
                     GIPSNMIRDTIERGGGSYIPLGEVSAQTYNRYANYLNSFTGVAVNPYTSRYYYDGGIA
                     PQVIGYLLSISPEQYEDYRRKGYAGDEKVGAAGIEKWGEEYLKGKPAGDLYVVNADGS
                     YDTLISKTDPKPPSMIYTTLDKDLQILVQKALYGFSGAIVVLEVDTGRVLAMASSPSF
                     DPNYFNPQNYNSQWALQDLVNDHARPLWNRAAQSAYPLGSVFKLVTAAAALESGLYTP
                     DSRYECTSQYTELPGFVGNDWTFDKKKPPSGNLTLVQGIMRSCNPWFYHLGLDLYRQK
                     GATYLSDWAREFGLGEATGIGAVAEEVGQINDPTTEGAAVQMGIGQGDMLVSPLQAAN
                     MVAAIANGGTLYRPQIIEKITSVDGAVLKQFEPEVLRKVPVSQETLQALKDGMELVVR
                     DQRGTAYTRFTGLRTPIFGKTGTATTSIEDPHSWFAGFTDANNPQKPDIAVAVILENA
                     GDGSVYAAPVFRRVIEAYFTGEISRYYWWETDYYVTRTPEPDTE"
     gene            complement(4944..5612)
                     /locus_tag="PLW25_03180"
     CDS             complement(4944..5612)
                     /locus_tag="PLW25_03180"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOS53524.1"
                     /translation="MSEPMIVLASNSPRRRQMLAWMEVPFTTTMAAIDETPRAGENPS
                     AYASRLAQEKARACERQAPFNALILAADTTVTDGSQIIGKPLNADDAARMLRQLRSRH
                     HMVHTAIVLALPSRGISELELCSTRVKMRAYTEEEIVNYIESGDPLDKAGAYAIQNET
                     FNPVEKISGCYASVMGLPLCHIERALRRLGFGVRKRVPYRCQKELAYACPVFKRILAG
                     ENVG"
     gene            5701..6273
                     /gene="xpt"
                     /locus_tag="PLW25_03185"
     CDS             5701..6273
                     /gene="xpt"
                     /locus_tag="PLW25_03185"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HOS53525.1"
                     /translation="MQALKERVAAEGKNLGDGILKVDSLINHQVDPKLMDLCGLEFAR
                     RFALTNPTRILTAEISGIAPALMTARHLNLPVVYARKSRPVTMPDTVYLTIAPSHTKG
                     NKVELIISPEYLKPGEKVLIIDDFLASGATILGLVRLTQAAGASIVGIGTLIEKTFEG
                     GREALQYLKVPIEALVQITSMEDGKIIFAP"
     gene            6288..7844
                     /gene="guaA"
                     /locus_tag="PLW25_03190"
     CDS             6288..7844
                     /gene="guaA"
                     /locus_tag="PLW25_03190"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOS53526.1"
                     /translation="MKTAIAILDFGSQYTQLIARRVRELQVYCEIYPWHTQPEAIHAL
                     NPAGFILSGGPSSVYEENAPTLPLFILRSGLPVLGICYGMQLLAEALGGMVSASPQKE
                     FGLAEIEVTSSNPLLKTGRQTVWMSHGDQVKEMPPGFYRLASSASTFIAAMADPARGI
                     YGVQFHPEVHHTPRGIEILRAFVNDICGLQPEWTPENIIASSVERIRRQVGGANVLSA
                     VSGGVDSTVATTLVHRAIGNQLRTVFVNTGLLRLNEAEQVEKVLKQHLGIPLVTVDAS
                     AGYFDALRGITDPEEKRRVIGAKFIEVFEQEAARAGNPPFLVQGTIYPDVVESSSAQG
                     SQAQRIKSHHNVGGLPESMKFELIEPLRYLFKDEVRRVGEALGIPSQLVWRQPFPGPG
                     LAVRCLGEVTPERVRRLQLADAIFREELETANLLNQKPAPGCALLAQAFVVLLPVRSV
                     GVMGDRRTYQETAALRAVTSEDFMTADWAHLPYDLLGRTANRIVNEVPGINRVVYDIT
                     SKPPSTIEWE"
     gene            7900..9792
                     /locus_tag="PLW25_03195"
     CDS             7900..9792
                     /locus_tag="PLW25_03195"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOS53527.1"
                     /translation="MRLPSRFITFTLVIMLLAVVFSSAAGQNPGSITVKKLDTSAYPL
                     ISATFTALDPSGGFVKDLKTGELWLSENSAAVTSYTLEMEQVGVRFFVAINEGPTLDN
                     RFSGVMRIDRIKTALFNWVAAQPAPALDEFHLVVNEGALQFNPLEPSSWYAALDHYQP
                     DLKTATPGMASLSAAVDNALNNSLQSPKATALLFITPLPTEVQLAAMQEIIARAKSNN
                     LHIFIWLIGPQDYGGSDTAVQLERIARDTGGQFIIFSGAEELPAIADLLDPFEFVYHL
                     SYKTANNISGEYSLVLEIKRGELVLASEPVTYELAVSPPNPIFLSPPSTIERSWSETK
                     KKSASVLIPEKVNLKILVEFPDGMQRELVNSRLFVDGGLADERSAPPFDTFTWDLSKY
                     TESGLHNLQVIIEDAAGLTGQTIQLPVEVKVAERKITAAERLLEQLTLVNGIIMGVIL
                     LVLLIGLVLLVKTLKKRIKKRPVKKPADPVTQPVNIEGEYTLAPPQPETTIQWPVIRG
                     VGLAPARLIQKRSAAPVVDYLPEIPLGNDEVLIGSERRKADYVLVHSTVSPLHARIYK
                     DADGNYRIADSGSAAGTWVNYAPVSTRGVQLEHGDLVQFGRLAYIFELHSAAPRRVKV
                     LPYQED"
     gene            complement(9846..10742)
                     /locus_tag="PLW25_03200"
     CDS             complement(9846..10742)
                     /locus_tag="PLW25_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOS53528.1"
                     /translation="MPISSPVTRSRRAMMYVPGSDENKIVKSATLGLDCAILDLEDGV
                     AFNRKDEARGVIRQALERVDFGRTERLVRINPLYSGRAEQDLAAVLPGKPDAILIPKA
                     DSAAIVVAVEKIISAFEQSQGWQEGEIALSLLIESASAFVNLAEICRASARVQALVFG
                     AEDFCADTGVTRTNSARELLYARSALVLHAAAFQLQAIDMVQINYRDTALLEAECRDA
                     VELGFSGKTVVHPSQVGPVQRAFTPSDSLIAYAREIVHGAELAQRGGSGVFTHQGKLV
                     DLPVVKRAEQILARARAAGIEV"
     gene            10806..11408
                     /locus_tag="PLW25_03205"
     CDS             10806..11408
                     /locus_tag="PLW25_03205"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HOS53529.1"
                     /translation="MNKPVKTLPAGYTLAGSVTLTRTADLLPMLIWSLVLPALLLLLV
                     VTVLCLTHQIADWNLFSLHSVGQIAAFFGGMLLVTLVMVIAHEGLHGLVFWCATGNLP
                     RFEFKFYYASASPGGWYLPRRVFMLATLVPLVTISLVCLALIPFASPFLRCLLILLAV
                     FNASGSAGDLVVALRLFRLPAETLSLDNGTSVYFFIPAPG"
     gene            11487..11574
                     /locus_tag="PLW25_03210"
     tRNA            11487..11574
                     /locus_tag="PLW25_03210"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11521..11523,aa:Ser,seq:tga)
     gene            11606..11698
                     /locus_tag="PLW25_03215"
     tRNA            11606..11698
                     /locus_tag="PLW25_03215"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11640..11642,aa:Ser,seq:gct)
     gene            complement(11761..12147)
                     /locus_tag="PLW25_03220"
     CDS             complement(11761..12147)
                     /locus_tag="PLW25_03220"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOS53530.1"
                     /translation="MKKRFVVVVFVVILLALAACSAPAAKLKLADSGSTLDLKNRQTF
                     TVALEGAPTTGYIWELKEIDPAVVELVGEPEYKSDSILIGSGGVFTYKFKTIAAGTSN
                     LKLVYYRPWKDNVEPLKVFMVQVNVK"
     gene            complement(12207..12863)
                     /locus_tag="PLW25_03225"
     CDS             complement(12207..12863)
                     /locus_tag="PLW25_03225"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HOS53531.1"
                     /translation="MLAWLIQSYQRLGLAGSLVILAGVLLSALFYRGKEGERYSITRH
                     FISELGELGVSRMAPLFNASLILAGLLFILMLIGVGFDMNTPWGYIAMSAGIVTAAAS
                     MCVGIFPMNQINPHTIAAMTYFRSGLVTVLLFSVAIILQPAARRVIPLYVLFFGVLSL
                     AAYTAFLLYAGRAAKKQAQNVLDTEKVRARPRFWWMAFLEWLVLISTIVWFLVVCSAR
                     "
     gene            13010..13783
                     /locus_tag="PLW25_03230"
     CDS             13010..13783
                     /locus_tag="PLW25_03230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS53532.1"
                     /translation="MYETLSSDYDRFVNWHNRLAYEMPFIERIVNQIQTGSPRSLHIL
                     DCACGTGMHAIALAQRGQQVSGADLFPQMIEISRQNAANAGVQVRFEVAGFDQLAAQF
                     GQAQFDLLLCLGNSLPHLLSPADLADALDDFALCLRPGGMLLIQNRNFDLVMQQKERW
                     MEPQVHREGNIEWLFQRFYDFEEDGLIRFNIVTLKNENGAGWQSSVASTRLRPQLHAE
                     LQSALSLAGFESIHPYGSLAGDPFSAASSGNLVLTAVRT"
     gene            13799..14911
                     /locus_tag="PLW25_03235"
     CDS             13799..14911
                     /locus_tag="PLW25_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53533.1"
                     /translation="MKIEPVSSKCDLQAFINLPYQLYRRDPTWVPPLRSEQRAQFSPQ
                     RNPMLRHCEYQLFLAREGDEVVGRISAFIDHLALEHWGQPIGLFGSFECVNDPRVSEG
                     LLASARAWLRAKDMRRMRGPWSFASQEWGLVVEGYQPEPVIMAPYNPPYYNDLLTDFG
                     LTAVKDLLVYVIDARENYVIPERILQLTQKVQQRYRVRVRPVNMKRLEEDVLTITRLA
                     NASICDNWGFYPVTEEEARAMARDMKQIVNPAAILIAEEENGSPIGFAMSLPDINQVL
                     KGLNGRLLPFGFFKMLLLLPRLHQYRMWALGVIPQWQGRAIDTLLYRATYDALYTDKL
                     RLEINYVLEDNDRMNNAISKLGARPLRRYRIYEAGI"
     gene            15062..15256
                     /locus_tag="PLW25_03240"
     CDS             15062..15256
                     /locus_tag="PLW25_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53534.1"
                     /translation="MYLHLDEEEKKILLDLLDTCISDLRQEIAGTDNINYKDMLKQRK
                     QVLIKLFQALQSDQQTQSMT"
     gene            complement(15305..16471)
                     /locus_tag="PLW25_03245"
     CDS             complement(15305..16471)
                     /locus_tag="PLW25_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS53535.1"
                     /translation="MSATVFYGSPRQARLEASESLPAKLDLILDRLHLRERVKGEWVC
                     LKLHVGNNIGYSTIHPVFVRKVVSAIKEGGGEPFIVDVDWDVAGSESRGYTREVLGCP
                     VYPSAGLHERYHYDHEWQYKNMKAWKVAGMVEDASFLVNFAHVKGHPMCGFGAAFKNI
                     ALGCMVGETRSQIHDTSHFDRYWFKGRCPDRSLMQQIVDSCPFGAVVFDKKDPEELHL
                     HFESCNQCMRCQQVAPPGSLLIQPENFHAFMEAAAISTAITLSTFEKGKTTHLALATH
                     MTPMCDCFGFTSMPILQDAGIFGSDDIVALEKAVLDKTTDLPLILENIPSSLEPQPHD
                     GHPFAQLHGRFKDPYVLLHYAEKLGLGSQDYQLVDVLPLDDLSPRTKGFYISAQ"
     gene            17118..18014
                     /locus_tag="PLW25_03250"
     CDS             17118..18014
                     /locus_tag="PLW25_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS53536.1"
                     /translation="MRYFVIKAEKVTKKYSHRPGIENITFEVEPGEIFGLLGNDHAGK
                     STLVSLLAGYFRPNSGHLMVFGLDSQRESRQIRERSGFFPGNYPAPSRHTVAELLRSQ
                     GHQPGGMLWTDIQKLTDQLDLDLAQPAATLSSHDRQVFDLLQAFIPRPELLVLDEPTY
                     ELKPAIQDVFYRLISEARQDGRTVFLTSRSLAEMERICDRVAIMHRGKLVAVERGIQL
                     RSRAIRVVEMRFADSISKDGFADIPNIRNLTIEGNKLRCVLQGEPDRLIKTACQHHIT
                     DLLSTQPSLEQVLADTYGIKTI"
     gene            18144..18923
                     /gene="udp"
                     /locus_tag="PLW25_03255"
     CDS             18144..18923
                     /gene="udp"
                     /locus_tag="PLW25_03255"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HOS53537.1"
                     /translation="MRNEEGLAFHLHIQQGDIGRYVLLPGDPARCEKIARYFKDAHFV
                     AQNREYVTWTGSLLGEKVSVISTGIGCPSAAIAVEEAIEAGADTLIRVGSSGSMQPHI
                     HTGDVVVVSAAIRDEGTTPYYLPIEFPAVADIEVVNALREAAERLGYKSHTGIAQSKD
                     SFYGEVERARMPMPARLAERWNAFITGGAACSEMESSALFILSSIYRKRAGGVMLVIN
                     ESDLAEVDEDNPAKHMAEFDADRVIKVAVEALKILIQSDRR"
     gene            18936..20165
                     /locus_tag="PLW25_03260"
     CDS             18936..20165
                     /locus_tag="PLW25_03260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS53538.1"
                     /translation="MKPTTLHSRITSLSRELALFITASFAVGMAASLVDSTLNNFLND
                     TFSLNGFQRSFLEFPREFPGVIVLFVSAALWFLGSRRLGAFAMLLAALGIFLIGFSSS
                     SYGLMVIFLFIYSNGNHLFMPVATTIGMELAADGRTGRRLGQLNAIRNLATIAGSFLV
                     FIGFKFLGFTYQHSFTIAALVFTAAAVLMFLMKPEKSAPPKTFLKLRKEYTLFYALAV
                     LAGSRKQIFITFAPWVIVTIFNKPTQTIATLVTIGGVIGILFQPFLGKAIDRYGERLI
                     LSAEALVLIFVCLGYGFSRSVFAENVAFLIVCVCYLLDQMLFSVSMARSTYIKKIALD
                     PSDIQPALSASISIDHVFSISVALLGGVIWNNFGFQYVFLLGMLIAMVNFMVARRIVI
                     PKPSITEKPVPQPIERD"
     gene            20270..21328
                     /locus_tag="PLW25_03265"
     CDS             20270..21328
                     /locus_tag="PLW25_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOS53539.1"
                     /translation="MKVNFSMWKKALSTLVKMDSKEEWDELDVVSKWLIATRSAVTIV
                     TLYSCAIGGLLAIRHGYFNDNWGMSILAWVIVTLGLFIAHGTNNFLNDYTDFARGVDT
                     ESYFRTQYSVHPLIQKFWSKEQQFRWFAISGTLAMLAGLFALFYTHFSLDIIILFAVG
                     ALVLLFYTWPLKHIALGELSIFLIWGPIIVAAVFLVLAKGSTEHVWPVALAAVPWGLS
                     VVSINLGKHIDKAPEDKKKMVGTLPVVIGEKAARITNIILLALIYLFILYLVFISRYF
                     SPILLIVFFAGQRMLYAIGVHTKPRPAEPPQDWPYWPTWFAGFTFYHNRLFSNLLLIA
                     ILIDTLLRLFLPGFWPAH"
     gene            21360..22754
                     /locus_tag="PLW25_03270"
     CDS             21360..22754
                     /locus_tag="PLW25_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOS53540.1"
                     /translation="MDIVLLSRWQFALTTIYHFFFVPLTLGLGWFVAILETMYARSGN
                     LLYKRLTKFWGKLFLINYAMGVVTGIVMEFQFGMNWSEYSRFVGDVFGAPLAVEGLLA
                     FFLESTFLGVWIFGWERLSKKLHAAVMWLVAIGGNLSAFWILAANGFMQHPVGYAIVN
                     GRAQMTDFGALLGNPRALYLFLHTFFAGLVTASFFMLGFSIYHLVKKQEVEAFRKSFV
                     LSALIGLLSTVAVGGAGHIQGVYLREVQPMAAAASEAHYETANPADFHLIAGIDQDGR
                     KTTWSVTIPRALSFLYFFKWEGEVEGLNQVQARYEQLYGPGNYIPNIALTFWSFRVMV
                     GVGLLMGLLSLGALVIAWKKYPEKWIERSGWLVRIILLPYLANTTGWVLTETGRQPWV
                     VHGLMQTKDAVSPNLTPATVLISLIGFVLIYAVLMVIDLYLLVKFAKKGLGAGDLDSV
                     PIAGEVEAKKKGGK"
     gene            22751..23770
                     /gene="cydB"
                     /locus_tag="PLW25_03275"
     CDS             22751..23770
                     /gene="cydB"
                     /locus_tag="PLW25_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOS53541.1"
                     /translation="MMNLNILWFVLVAVLYAGFFFLEGFDFGVGMLLPALGRKDVERR
                     AIINTIGPHWDGNEVWLIVAGGATFAAFPQWYATLFSGFYLGFFLLLFALIVRGVGFE
                     FRSKLSAPRWRYGWDWAIAVSSFVAAFLLGVVFTNLVRGVPINAAMQYTGGFFTLLNP
                     ISLLGGLTVVMLFLFHGANFLTLKLSDGLRERARNATRTYFTAVLLFALGLFAALLFT
                     PSAFSTKGVFSAVLPVLAAASLVVSRVMMGKKKEGWAFICSSLAVVLTVAALFTALFP
                     NVMVSTTNPANNLTIYNASSSDYTLKVLSIIAAVLVPVVLAYQTWTYVIFRKRVKADT
                     KTLTY"
     gene            complement(23804..24508)
                     /locus_tag="PLW25_03280"
     CDS             complement(23804..24508)
                     /locus_tag="PLW25_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOS53542.1"
                     /translation="MQTIGLIGGMSWESTLEYYRILNLETKKRLGGFHSARCVLYSVD
                     FAEIEEWQRQGNWQEAARCLLAAGRALQAAGADFILLCTNTMHKLAPSLEAELGIPLL
                     HIADATADAVKAAGITQVGLLGTRFTMEQDFYRDRLAKKHGLNVLVPGEAERQIIHEV
                     IYQELCMGVVKASSREKFSKVISNLADAGAQGVILGCTEIENLVRPQDSVLPIFATTK
                     IHAEAAVNLAIKNRGN"
BASE COUNT         5384 a         6865 c         6527 g         5874 t
ORIGIN      
        1 caaggtcatg ggtgatccag atgatagcca ttcccagttc ttcgcgcaag tgtttgacca
       61 ggtcaacgat ttgcgcctgg atggtcacat ccagcgcggt ggttggctcg tcagcgatga
      121 ggatttgcgg gttgcaggaa agcgccatgg cgatcatcac gcgttggcgc atgccgccag
      181 aatactggtg agggtaatct ttcagtcggt cagccgcgtt tggaattcct accagcccga
      241 gaagttcgat ggaacgttta cgcgcctgtt ctttggtgag attcaggtga agcatcaagg
      301 gttcagccat ctgcttgcca atgctcatca ccgggttgag agatgtcatc gggtcctgaa
      361 agaccatgct gatctgccct ccccttacat ggcggatttc atcaggggtc atttttaaca
      421 ggtcgttccc tttaaagaat gcctcgccgg ctatgatctt cccgggtggt gcagggatga
      481 gacgcaggca cgatagcatg gtaacgctct tcccgcatcc gctttcaccg acaaaaccca
      541 gggtttctcc ctctttaatg tgaaaggaaa caccgttgac agcgtgcacg gtgccctcag
      601 gggtcttgaa gtgagtttct aaacctttga cttttaataa tggctcaccc atttacagat
      661 ccttgcaaca aaaatgatcg catcttaaaa agcaggtatc ttgttagttt accataaaca
      721 gatggttttt tggctggaaa aattaatcgg gaaaaatgat ggtttttcat aagaaattaa
      781 attggcggat cttcattcac agaagtgctc atatgcttgt ctgtgctccc cagccggatg
      841 gctgggtttt caaagatgcg gctgcaggta accagctggt tataaatgac agggcatttc
      901 acccactgtc cgttaaagtc caccaacaca tccacctggc ccagcatctc atcgtaattg
      961 ctcataatca aaccatgttt ctggcttacc acgtctttca catggtcgaa agcgaacagg
     1021 cgcattttaa gcgctgtttc ttgcggcacc tcctgtcctt catgcgccag tatgaaatcg
     1081 atcacttcat ccacctgctg aacggactct ctgaaccttt tgttgaggat tgaatcgcta
     1141 aagttaccca ccaggctaaa tgaaagaaaa caaacaacca gtggaacgat taaggagaag
     1201 gtacctgatg aaaacagcgc ctgttcgatg agaatattct cgattagacc gataatcagc
     1261 gaaaccaaag cgatgaccgc aaaactgatc cacaggttct ggtataactc gtgatggaat
     1321 ggatactgta aaaaatcgcc aagagcaggg ttaaatataa aaatgatccg gggagccacc
     1381 ttgatcggca gccagaccac caggtgggca aataaaagcg cctgcaataa ccaaagcaag
     1441 aggctgaacc aggcgcgctc aaccctcgcg gtcaaccagc cgaccagcgc actgctcacc
     1501 agcccaatga ctaaacccgg aacaaaattc acccacggaa agactccgtg cgcgcaggcc
     1561 aataaaaacg cgtccactcc ccaggtaaaa atggcgaacg ccagcccggc agcaaggccg
     1621 taaagcagcc ctgctttttg ttttcgtttg ctcactgctg gtttcatctt taccaacctc
     1681 aaacccgtgc aaccggtgac cctggttcgc tatcgatgtg tggaggagag attttcatca
     1741 tacaccggtt tttaaaaaag tcaacatttc ctggtttgca acaggcgtca cccgggcagg
     1801 gggagcctct ttctggtgat tgatgtcgaa tgttgcgggt tattcatcgc ctacgcgcat
     1861 gcgcaggtaa gattcgtatc tttctgcgct aaccttgccc gcttcgaccg cctttattac
     1921 agcgcagccg ggttcttcat aatgggcgca gtcgttgaac tggcagtgtt tcaccagttc
     1981 gcgcagctcc ggaaaatacc catccagctc ttcaggctcg gtatcccaca gcgagagttt
     2041 gcgcagccct ggcaggtcag ccacgtaccc gccgccatcc agagggaata attctcgcac
     2101 caccgtggtg tgcctgcccc tgtctttacg cgcgctgacc tcctgcaccg ccagctccaa
     2161 gccgggttgt acgcggttta aaaggctaga cttgcccaca cctgaaggac cggtgagcgc
     2221 tgatactttg ccggatagcg cctcgcgcaa ttcatctact ccttcaccgg tgaccgcgga
     2281 agtgtagata acccggtaac ccaacggcgg gtagatgctg aatatccgcc gggcgttgcg
     2341 cttacctacg aggtccagtt tgttggcaac aacaaccgcg cccaacccct gtttttcagc
     2401 gatgaccagg aaacggtcga gcatgcgtaa atgcggttca ggtgaagcgc aggcgaaaac
     2461 cgctacgatc tggtttgggt tggccagtaa gatctgccgg tatgttccgc gcggggtggg
     2521 atccaggcgc gccagttctc gcgcgcgagg ttcgatggct tcaatcaccc cgctgccgtc
     2581 gggaaggcgg gtaatggcaa cgttatcacc gatgctgatc acgtctccct ggaaagagcg
     2641 gcgcttcagc cgtccgcgca gtttacacac aatcatcccc ccggctgttt gtacgttgta
     2701 gaagccggat tggaaacggg tgacaatgcc tttcagggtt tccgcgctca catttattcg
     2761 gtatcaggct cgggggtgcg ggtcacataa taatctgttt cccaccaata gtacctgctg
     2821 atctcgccgg taaagtaagc ctcgataaca cgcctgaaaa ccggcgcagc gtaaactgag
     2881 ccatcacccg cgttttccag aatgactgcc accgcgatat ctggtttttg cgggttgttc
     2941 gcatcagtaa agccggcaaa ccaggagtgg gggtcttcta ttgaagtggt agcagtaccg
     3001 gttttaccga aaatcggggt cctcaagcca gtgaagcggg tgtaagccgt gccgcgctgg
     3061 tcacgcacca ccagttccat tccatctttc aacgcctgca gggtttcttg gctgaccggg
     3121 accttgcgaa gcacttctgg ctcgaactgt ttcaggacag cgccatccac gctggtgatc
     3181 ttttcgatga tttgcggtcg gtaaagcgtg ccaccattgg caattgccgc caccatgttg
     3241 gccgcctgca gcgggctcac cagcatatct ccctggccaa ttcccatttg caccgctgcg
     3301 ccttcagtgg tgggatcatt gatctgcccc acctcttccg cgacagcgcc aatcccggtg
     3361 gcttcgccaa gtccaaactc gcgcgcccaa tcagaaagat aggtcgcgcc cttctgtcgg
     3421 taaaggtcca gccccaggtg ataaaaccag gggttgcagg aacgcatgat tccctgcacc
     3481 agcgtgaggt tgcctgaagg tggtttcttt ttatcgaacg tccagtcatt gcccacaaag
     3541 cccggcagct cggtgtattg gctggtacat tcatacctcg aatctggtgt atacaacccc
     3601 gattcgagcg ctgcggctgc cgtgaccagt ttaaacacag accccagcgg gtaagcggat
     3661 tgtgcggcgc gattccacag cgggcgtgcg tggtcattca ccaggtcctg cagcgcccac
     3721 tggctgttat agttttgcgg attaaaataa tttgggtcga aactggggga agaagccatg
     3781 gcaagtacac ggccggtatc cacttccagt acaacaatgg cgcctgagaa gccatagagc
     3841 gctttttgca ccaggatttg caggtcttta tccagggtgg tgtagatcat actgggtggt
     3901 tttgggtctg tcttactgat caaagtatcg taggaaccat ccgcgttgac cacataaagg
     3961 tcacccgcgg gtttgccttt gaggtattct tcaccccatt tttcaatccc ggctgcgcca
     4021 accttctcgt ctccggcgta gcctttgcgg cggtaatcct catattgttc tggtgaaatg
     4081 ctcagcaggt acccgattac ctgaggggca attccgccgt cgtaataata acgcgaggtg
     4141 tagggattga ccgccacccc ggtgaaggaa ttcaaataat ttgcgtagcg gttataagtt
     4201 tgcgctgaaa cttcgcccag tggaatatag gagcctcctc cacgctcaat ggtatcgcgg
     4261 atcatatttg aaggaatgcc cgtcataacc gtcaggatcc cagccaggtt gcccacctgg
     4321 tcatccgcga tttcatccgg aaccaccccg attgccacag cttcggcttc agcagcgatg
     4381 atattgccgt cattatcata aatatcacct cgcgtggggg aggtgagact gagcgcgagc
     4441 tttttccctc cgctcagttc aggcatgatc aatccgcttt cccactgtat ccaccattta
     4501 ccgtgatctg ccagcaggcc catttccatg cggcggatga tatcgccgaa caaactggtt
     4561 ttaaaagaca cctggtaacc caccttcgcc gaggtgggat tgaccagcgt ggtgaggatg
     4621 ccgtattcaa tttcccgtaa ggtcatattg agagcgacat ccgtataggt tttcacgaaa
     4681 tccgcttcag aaatggcatc gcggctggta ggcgccagca tggcgtacat gccgctgtaa
     4741 tcctgctgat tccatttttc caggtattcc agcacggtca gtgaaacgtc aggcaattga
     4801 gtggtttgca cgataggcga aggcagctcg ccgcctggtt caatggtggg ttgcgcgcca
     4861 ttgctacaag cggttaaaac caaaagagct gtgaccagaa tctgccaggg aaatttaaaa
     4921 tggtttattt tcatagtttc tctttaaccc acattctcgc ctgctaaaat gcgtttgaat
     4981 accgggcacg cataagccag ttccttctgg caccgataag gcacgcgctt gcgtaccccg
     5041 aaccccagtc gcctgagcgc ccgctcgata tggcacagcg gcaggcccat gacgctggca
     5101 taacaaccag agattttttc aacgggattg aacgtttcat tctgaatggc gtaagcaccc
     5161 gctttgtcca gtgggtctcc tgattcgata taattgacaa tttcttcttc ggtgtaagcc
     5221 cgcattttta cccgcgtgga gcaaagttcc agttctgata taccgcgtga gggcagcgcc
     5281 aaaacgatgg ctgtgtgaac catgtggtgg cggctgcgta actggcgcag catgcgggca
     5341 gcgtcatcag cgttgagcgg tttaccaata atttggctgc catctgtcac ggtggtatcc
     5401 gctgccagga taagcgcatt aaaaggtgcc tggcgttcac aggcacgggc tttttcctgc
     5461 gccaggcgcg aggcataggc tgaagggttc tcaccagcac gtggtgtttc atcaatagca
     5521 gccatggttg ttgtaaaagg cacctccatc cacgccagca tttgccgccg tcgcggtgaa
     5581 ttggatgcca ggacgatcat tggttcactc atagaaaata ttctaacaca gcccctgccg
     5641 gtggggcgtt tacagtctta tcgtgtaaaa tggactatat catttttccg ggagggatga
     5701 atgcaagcac ttaaagagcg tgtggccgcg gaaggaaaaa acctggggga tggtattctc
     5761 aaggtggata gtcttatcaa tcaccaggtg gatcccaagt taatggacct ttgcggattg
     5821 gagttcgcgc gccgcttcgc gctcaccaac ccgacgcgca ttttaaccgc tgaaatttcc
     5881 ggcattgcgc cagcattgat gaccgcccgt cacctcaacc tgccggtggt gtacgcgcgt
     5941 aagagccgcc cggtaaccat gcccgatacg gtgtacctca ccatcgctcc ttctcacacc
     6001 aaaggtaaca aggtagagct gatcatctca ccggaatacc tgaaacccgg agagaaagtg
     6061 ctgatcattg atgattttct cgcctctggg gctaccattt tggggctggt acggctcacc
     6121 caggctgccg gcgccagcat cgtgggaatc ggcaccctca tcgaaaaaac tttcgagggc
     6181 ggaagggaag cgctgcagta cctcaaagtc cccatcgaag cgctggtgca aatcaccagc
     6241 atggaagatg ggaaaatcat ctttgctccg tagtctatcg ttcgcttatg aaaacagcca
     6301 tcgccatact ggattttggt tcacagtaca cacagctaat cgcgcgaagg gtccgagaat
     6361 tgcaggtgta ttgtgaaatt tacccatggc atactcagcc agaagccatt catgcattaa
     6421 accccgccgg gtttattctt tccggcggac cgtcttctgt atatgaagag aacgcgccaa
     6481 cgttgccgct gttcatcctg cgctccgggc tgccggtgct gggcatctgt tacggtatgc
     6541 aattgctggc tgaggcttta ggcggtatgg tgagcgcatc tccgcagaaa gagttcggtc
     6601 tggcagaaat cgaagtcacc agttctaacc cgctgcttaa aactggcaga cagactgtgt
     6661 ggatgtcgca cggcgaccag gtgaaagaaa tgcccccggg tttttaccgg ctggcttcct
     6721 ccgccagcac tttcatcgcc gcgatggctg atcctgcgcg cggtatctat ggtgtgcagt
     6781 tccaccccga ggtgcatcac accccgcgcg ggatagaaat actgcgcgct ttcgtgaacg
     6841 atatctgcgg gttgcagcca gagtggacgc cggagaacat catcgcgtca tctgttgagc
     6901 gtatccgccg ccaggtgggc ggcgcgaacg tgctttccgc ggtcagcggc ggagtcgatt
     6961 ccaccgtggc aaccacgctg gtacatcgcg ccatcggcaa ccagttgcgc accgtctttg
     7021 tcaacaccgg cttactgcgg ttgaatgaag ccgaacaggt ggagaaggta ctcaaacaac
     7081 atcttggtat cccgctcgtt acagtagacg ccagcgccgg ttattttgac gctctgcggg
     7141 gtatcacgga tcctgaagaa aagcgccgtg tcattggcgc gaagttcatc gaggtgttcg
     7201 agcaagaggc cgcccgcgcc ggcaacccgc cgttcttagt gcagggcaca atttacccgg
     7261 atgtggtgga atcttcttcg gcgcagggca gccaggcgca gcgcatcaag tctcatcata
     7321 atgtcggcgg gctgcccgaa agtatgaaat tcgagttgat tgagccgctg cgctatcttt
     7381 ttaaagacga agtgcggcgc gtcggcgaag ctctgggtat cccttcgcag ctcgtctggc
     7441 gccagccttt ccctgggcca ggtttggcgg tgcgctgcct gggcgaggtg acccctgaac
     7501 gggtgcggcg tttacagcta gctgacgcga tcttccgcga agagctggaa accgccaacc
     7561 tgttaaacca aaaacccgcg ccgggctgcg cgcttttggc gcaggctttc gtggtgttgt
     7621 tgcctgttcg ttcagtggga gtgatgggag atcggcgcac ctaccaggaa accgccgccc
     7681 tgcgcgcggt gacttcagaa gattttatga ccgcggattg ggcacacctg ccttacgacc
     7741 tgttggggcg cacagccaac cgcatcgtca acgaggtgcc cgggatcaac cgggttgttt
     7801 acgacatcac ctctaaaccg ccatccacca tcgagtggga gtgattctta ttaacagcgg
     7861 ggttttcgct tacaatagtt gataaggaac attctgagca tgcgcttacc aagcaggttc
     7921 atcaccttca ccctggtcat tatgctgctg gcggtggtgt tttcttccgc cgcggggcaa
     7981 aaccctggca gcatcacggt taaaaaattg gataccagcg cgtacccgtt gatcagcgcc
     8041 actttcacag cccttgaccc cagcggcggg tttgtgaaag acctcaaaac cggtgaactg
     8101 tggttaagcg aaaacagcgc tgctgtcacc tcttacaccc tcgagatgga acaggtgggc
     8161 gtgcgttttt ttgtcgccat caacgaaggc cccacgctgg ataaccgttt ttctggagtg
     8221 atgcgcatcg accgcatcaa gaccgcgttg ttcaattggg ttgcggctca gccagcccct
     8281 gcgctggacg aatttcacct ggtggtcaac gagggggcgc ttcaattcaa cccgcttgaa
     8341 ccctccagct ggtacgccgc gctcgatcac tatcagccag acctgaagac cgccactccc
     8401 gggatggcga gcctttctgc cgcagtggat aacgcgttga acaacagcct gcaatctccc
     8461 aaggccacag cactgctctt catcaccccc ctgcccacgg aagtgcagct cgccgccatg
     8521 caagagatca ttgcccgcgc caaaagcaac aacctgcaca ttttcatctg gttgattggc
     8581 ccacaggatt acggtggcag tgatacggct gtgcagttgg agcgtatcgc gcgggataca
     8641 gggggacagt ttattatttt ttctggggct gaagaactgc ctgccattgc tgatttgctc
     8701 gatccattcg aattcgtgta tcacctcagc tataaaaccg ccaacaacat cagcggagag
     8761 tacagcctgg tgcttgaaat caagaggggc gagctggtgc tggcttcaga accggtaaca
     8821 tacgagctgg cggtatcacc gcccaacccc atcttccttt cgccacccag cactatcgaa
     8881 cgtagttgga gcgagaccaa aaaaaaatct gcttctgtgt tgatcccgga gaaggtcaac
     8941 ctgaaaatcc tggtcgagtt tccagacggg atgcagcggg agctggtgaa cagtcgtctt
     9001 ttcgtggatg gtggcttggc agatgaaagg agcgccccgc cctttgacac attcacctgg
     9061 gatctctcaa agtacaccga aagcggcctg cacaacctgc aggtcatcat cgaagacgct
     9121 gccgggttaa caggtcaaac catccagcta ccagtagaag tgaaggtcgc cgagcgaaaa
     9181 atcaccgctg cggagcgcct gctcgagcag cttacgctgg tcaatggcat catcatgggc
     9241 gttatcctgc tggtgcttct catcgggctg gtattgttgg taaaaaccct gaaaaagaga
     9301 attaaaaaga gacccgtcaa aaaacccgct gatccggtca cgcagccggt aaatattgaa
     9361 ggagaataca ccctcgcgcc gccacaaccg gagactacga tccaatggcc ggtcatccgc
     9421 ggggttgggc tggcgcccgc tcgattgatc cagaagcgct ctgctgcgcc ggtggtggat
     9481 tatctgcctg aaattccgct gggaaacgat gaggtgctta tcggctctga acgccgtaaa
     9541 gcggattacg tcctggtgca cagtacggtt agcccgctgc acgcgcgcat ctataaagac
     9601 gctgacggca attatcgcat cgcggattca ggttccgccg cgggcacatg ggtcaactac
     9661 gcgccggtat ccaccagggg ggtacagttg gagcatggag acctggtgca gttcggccgg
     9721 ctggcgtata tttttgaatt gcacagcgcc gcgccgcggc gggtgaaagt gctgccatat
     9781 caagaagact gatcaataat cattgtaacc gcagcagagg gcagcgccct ccggctggtc
     9841 tattttcaca cctcgattcc agctgccctg gcgcgcgcca ggatttgctc cgcccgtttt
     9901 accaccggca ggtcaaccag ttttccctgg tgagtgaaca caccgctgcc gccgcgctgg
     9961 gcgagctcag ccccatgcac aatttcacgc gcgtaagcga tgagagaatc cgagggcgtg
    10021 aacgcgcgct gcaccggccc aacctgcgaa gggtgcacca ctgttttacc gctgaatccc
    10081 agctccaccg cgtcgcggca ttccgcctcc agcagcgctg tatcccggta gttgatctgc
    10141 accatgtcga tggcttgcag ttgaaaagcc gccgcgtgca gcaccagcgc gctgcgcgcg
    10201 tacaacagct cgcgcgccga atttgtacgc gtcaccccgg tatccgcgca aaaatcctct
    10261 gccccgaaga ccagtgcctg cacgcgcgcg ctcgcccggc agatctccgc caggttcaca
    10321 aacgcagacg cgctttcaat gagcagcgac agggcgatct cgccctcctg ccagccttgc
    10381 gactgttcga acgcgctgat gatcttctcc accgccacga cgatcgccgc gctatctgct
    10441 ttgggaatga gaatagcatc cggtttgccc ggtaacaccg ccgccaggtc ttgctcggcc
    10501 ctgccgctgt acagcggatt aatccgcacc aggcgctcag ttcgcccgaa atccactcgt
    10561 tccagcgcct ggcgaatcac accgcgggct tcatctttgc ggttgaaagc caccccgtct
    10621 tccaggtcaa ggatggcgca gtccagccca agcgtggctg atttcacgat tttattttca
    10681 tcactgccgg gcacatacat cattgccctg cgcgagcggg tcacaggtga agatattggc
    10741 atggtcgctg ctcctgttag aaatattggc ttgcgataaa atgatactac atggaggaaa
    10801 acccgttgaa taagcccgtg aaaacgcttc cagcaggtta caccctggcc ggctccgtca
    10861 ctctcaccag aaccgctgat ctccttccta tgcttatctg gagcctggtg ctgcccgcgc
    10921 ttttactcct gctggtggtg acggtgttgt gcctcaccca tcaaatcgcc gattggaacc
    10981 tgttcagcct ccattcagtt ggtcaaatcg ccgccttttt tggtggaatg ttgctggtga
    11041 cgctggtgat ggtcatcgcg catgaagggt tgcacgggct ggtattctgg tgtgccactg
    11101 gcaatctacc caggtttgag ttcaagtttt attatgcttc agccagcccc ggcggctggt
    11161 acctgccacg gcgggtgttc atgctggcca cgctggtccc cctggtcacc atcagtctgg
    11221 tatgcctggc gctgattcca tttgcttccc cattcttacg ctgcctgctc attttgttag
    11281 cggtgttcaa cgcgtcaggc tcggctggtg acctggtggt tgcgctgcgt ctgtttaggc
    11341 tgccggcaga aacgctcagc ctggataatg gcacttcggt gtactttttt atccccgcgc
    11401 ctggttgaga gggttgattc acaacccggt tttgcttgcc cgcgggtgtc actcgtgata
    11461 aaatgacaat ccctgcgcag gaacaaggaa gggtggccga gtggtttaag gcgcctgtct
    11521 tgaaaacagg tttggtgaaa gccaacgtgg gttcgaatcc ctccccttcc gccagttact
    11581 tttcgccggg aacaaccgca tacacgggga ggtgccggag tggctgaacg ggatcgcctg
    11641 ctaagtgatt gtcgggcttt aaactcgacc gggggttcga atccccccct ccccgccatt
    11701 tttacacagg aggttttcca gcctgtgttt tttgttcatt cgcttatttt gtttgcgaaa
    11761 ttatttcaca ttcacctgca ccataaacac ttttaacggc tcaacattat ccttccatgg
    11821 acggtaatag accagcttga ggttactcgt tcccgcggcg atggttttaa atttataggt
    11881 aaacacgccg cctgaaccga taagtattga atctgacttg tactctggtt cgccaaccag
    11941 ctccaccacg gctggatcga tctccttcaa ttcccagatg tagccggtgg tgggtgcccc
    12001 ctccagagcc actgtgaatg tttgcctgtt tttcaggtca agcgtggagc cactatccgc
    12061 cagttttaat ttcgcggccg gcgcgctgca tgccgccaag gcaagcaata tcaccacaaa
    12121 cacgacaacc acgaaacgtt ttttcatctt tacctccgga gtattcctta tcaccaagac
    12181 gggttgggtc gtattttcgt tccacgctag cgggcgctgc aaaccaccag gaaccagacg
    12241 atggtggaga ttagtaccag ccactccaaa aacgccatcc accagaagcg tggacgtgcg
    12301 cgcacttttt ctgtgtccag cacgttttga gcctgttttt tcgccgctct gccggcgtaa
    12361 agcaagaaag ccgtgtaggc agccagagat aaaacaccaa aaaagagcac atacagcgga
    12421 atcaccctgc gagcagccgg ctgcaggatg atggccacgc tgaacagcag caccgtcacc
    12481 aatccggagc ggaaatacgt catcgccgcg attgtgtgcg ggttgatctg gttcatgggg
    12541 aaaatgccaa cgcacatact ggcagccgcg gtgacaatgc cggcgctcat ggcaatgtac
    12601 ccccaggggg tattcatatc aaaacccaca ccgataagca tcaagatgaa gagcagcccc
    12661 gcgaggatca gagaggcgtt gaaaagcggc gccatgcgag aaacacccag ctccccaagt
    12721 tcagaaatga aatgacgtgt aatcgagtaa cgctcaccct ctttaccgcg ataaaaaaga
    12781 gccgagagca gcacccctgc caaaatcacc aggctgcccg ccagccccag cctctggtaa
    12841 gattgtatca accatgcaag cattgctgaa gtccctccca agaacagctc gtgaaattga
    12901 cccttgtttc tggtattcta aaatagatgg tttgccagta tcataaccca taaactggct
    12961 ctgacttctt ttttaagttc cttaaaaatt cgcatgcacg gagtgttcta tgtacgaaac
    13021 cttatcatca gattatgacc gttttgttaa ctggcataac cgcctggcct atgaaatgcc
    13081 tttcattgaa agaatagtca atcaaataca aacgggcagc ccgcgttcgc tccatatcct
    13141 cgactgcgcc tgcggcaccg gtatgcacgc catcgcactg gcgcagcgtg gacagcaggt
    13201 cagcggggct gacctgttcc cgcaaatgat agaaataagc cgccaaaacg ctgccaatgc
    13261 cggcgttcag gttcgtttcg aggttgccgg gttcgatcag ctggcagcgc agtttgggca
    13321 ggctcaattc gacctgctgc tttgcttggg gaactcgctt cctcacctgc tcagcccagc
    13381 agatctggct gacgcgttgg acgatttcgc cctctgtctg cgccctggag ggatgctgct
    13441 catccaaaac cgcaattttg acctggtcat gcagcagaag gagcgctgga tggagccgca
    13501 ggttcaccgc gaaggaaaca tcgaatggct tttccagcgg ttttacgatt ttgaagagga
    13561 tggactgatc cgcttcaata tcgtgaccct taaaaatgaa aatggcgctg gctggcagtc
    13621 atccgttgcc agcacccgtc tgcgcccgca attacacgcc gagttgcaaa gcgctttgag
    13681 ccttgccggt tttgaaagca tacaccccta tggaagcctg gcaggcgacc cgttctcagc
    13741 tgcttccagt ggtaacctgg tgctgacggc ggtgcgcact taaaaaaagg gaggcagtat
    13801 gaagatcgag cccgtcagca gtaagtgcga cctgcaagcc tttatcaacc tgccttatca
    13861 gttataccgc cgcgacccta cctgggtgcc gccactgcgc tcagaacagc gcgctcagtt
    13921 ttcgccgcag cgcaacccca tgctcaggca ttgcgaatac cagctctttc tggccaggga
    13981 gggggatgag gtagtggggc gaatctccgc tttcatcgac cacctggcgc tggaacactg
    14041 gggacagccc attgggctgt ttggttcttt tgagtgcgtg aacgacccga gagtctcaga
    14101 agggttactc gccagcgcgc gcgcctggct gcgcgcgaag gatatgcgcc ggatgcgcgg
    14161 tccgtggagt tttgcttcac aggagtgggg gctggtggta gaaggctacc aaccagaacc
    14221 ggtcatcatg gcgccttaca atccgcctta ttacaatgac ctgctcaccg atttcgggct
    14281 tactgcggtt aaagacctgc tggtatacgt gatcgatgcc cgggaaaatt acgtcatccc
    14341 cgagcgtatt ctacagttga cgcaaaaagt gcaacagcgt taccgcgtgc gtgtgcgccc
    14401 agtgaatatg aaacgcctcg aagaagatgt gcttaccatt acccggctgg caaacgcctc
    14461 catctgtgac aactgggggt tctatccagt aaccgaggaa gaagcgcggg caatggcgcg
    14521 cgacatgaag caaattgtca accccgccgc catcctcatc gccgaagagg aaaatggcag
    14581 cccaattggc ttcgccatga gcctgcctga tatcaaccag gtgctcaaag ggcttaatgg
    14641 gcgcctgttg ccttttgggt tctttaagat gcttttactg ctgccgcgct tgcaccagta
    14701 ccgtatgtgg gcgctggggg taattccaca gtggcagggc agggccattg acaccctgct
    14761 ttaccgcgcc acttacgacg cgctctatac cgataaactc cgcctggaga tcaattacgt
    14821 cttggaagac aacgaccgta tgaataacgc catcagcaag cttggtgcca gacctctacg
    14881 gcgttatcgc atttacgaag caggtattta agctcgcccc caatcttgac ttatgccggt
    14941 gaagttgcta tgctgaaata aacagcaccc ttcccgaatt catcgtggta tgttcgccag
    15001 gttaatacat cgcgcagctg ttccatactg aacggtcttt atttatgaaa ggaggagcaa
    15061 gatgtatctc cacctcgatg aagaagaaaa gaagatcctg cttgatttat tggatacctg
    15121 tatttcggac ctgcgccagg aaatcgccgg cacagataat atcaactata aagatatgct
    15181 caagcaacgc aagcaggtgc tcatcaagct atttcaggcg cttcaatctg accagcagac
    15241 ccagtcaatg acctgaaaca ccagtgtaag cggcttgccc cggtgttcat aaccttcacc
    15301 gcagtcactg cgctgagata taaaaacctt tggtcctcgg gctgaggtca tccagcggca
    15361 gcacatctac cagctggtaa tcctggctgc cgagccctaa tttttcagcg taatgcagca
    15421 gcacataagg gtctttgaac ctgccatgca gctgcgcgaa aggatggccg tcatgtggtt
    15481 ggggctccag gctgctgggg atgttctcca atatcagcgg caggtctgtg gtcttatcca
    15541 ataccgcttt ttccagcgcc acgatatcgt ctgagccaaa aatgccagcg tcttgcagga
    15601 tgggcataga ggtaaagccg aaacaatcgc acatgggcgt catgtgtgtg gccagcgcca
    15661 gatgagtggt ctttcccttc tcaaaagtgg aaagggtgat ggctgtcgaa atggcggcgg
    15721 cttccataaa agcatgaaaa ttttccggct gaatgagcag gcttcctgga ggtgccacct
    15781 gctggcagcg catgcactgg ttgcaggatt caaaatgcag gtgaagttcc tcggggtctt
    15841 ttttatcaaa aaccaccgcg ccaaaggggc aggaatcaac aatctgctgc atcagcgacc
    15901 ggtcagggca acgccctttg aaccagtagc ggtcgaaatg gctggtatcg tgaatttgac
    15961 tgcgggtttc gcctaccata caaccaagcg cgatgttctt gaaggcagcg ccgaagccgc
    16021 acatggggtg acccttgacg tgagcaaaat tgaccaagaa actggcgtcc tccaccatac
    16081 ccgcaacttt ccaggccttc atatttttgt attgccattc atgatcgtaa tggtagcgtt
    16141 cgtgtaagcc agcgcttgga tacaccgggc agcccagcac ttcgcgcgtg tagccgcgcg
    16201 actcgctgcc cgccacatcc caatccacat ccacgatgaa aggctctcca ccaccttctt
    16261 tgatggcgct taccactttg cgcacgaata ccgggtggat ggtggaatag ccgatattgt
    16321 ttcccacgtg cagtttcagg catacccatt cacctttcac ccgttcgcgc aggtgcagcc
    16381 tatccagtat gagatcaagt ttcgccggta atgattcgct ggcttccagc cgtgcctggc
    16441 ggggtgaacc ataaaaaaca gtggctgaca tggcagaatt cctttcttcc ttgaggataa
    16501 tgaaattata tcaacgaccc aaatcgcgtg ccagactctt atgcccggcg ggtgggtaag
    16561 cgattacaaa gaagcgatac cgcacactgt tccaccaggt tgaacctgcc ttttagcgcc
    16621 gctgtgccag taagcaagtt acccgcgcgg tgaagacaaa agcggtacgc tcggtttcgt
    16681 atctgatgcg cccttcgccg ggggcatttt aacttggcgc taacaaactg ctgcaaacct
    16741 cttgacttta ccgttttgag agaataaaat gggctcattg aagtcacact gaagttagag
    16801 cctctctcca tacagaaagg gttccagatg caatcggaag aaacgggcag taccaaggat
    16861 gtacctcctc tcagtaccaa gaggatctta gtcctgtcca cgtacgttga aaagaataag
    16921 gcgcctgaga gaccacaacg gtcggggcgc gcaaagggag tacccgcctg tctcgtatga
    16981 gctgattgat caaggcacgc ttacgctgcc ggccggtgct tccgccacca gccggttttt
    17041 gtttccggcg ttaagaccac aaatcatcca gtggcaaacc gcgaccacgg tgaaccactt
    17101 catttttgga ggccggcatg cgctattttg tcatcaaagc ggaaaaggta acaaaaaaat
    17161 acagtcaccg cccaggtatt gagaatatta ccttcgaagt ggaaccaggt gagatcttcg
    17221 ggctgctggg caacgaccac gccggaaaaa gtaccctggt gagcctgctg gcggggtatt
    17281 ttcgccctaa cagcggtcac ttgatggttt ttggcctgga cagccagcgc gagtcccgtc
    17341 aaattcgcga acgcagtgga ttttttcctg gcaattaccc cgctcccagc cgccacacgg
    17401 tggctgaatt actgcgttca caggggcacc aaccaggcgg catgctgtgg acggatatcc
    17461 agaagctgac cgaccagttg gacctggacc tggcacagcc agctgccacc ctttccagcc
    17521 acgaccgaca ggtgttcgac ctgctgcagg cgttcattcc acgccctgaa ttgctggtac
    17581 tggatgaacc tacttatgaa cttaaacccg ccatacagga tgtgttttac cggttgattt
    17641 ctgaggcgcg ccaggacggg cgcaccgtct ttctcacctc gcgctcactg gctgagatgg
    17701 aacgcatttg cgaccgcgtg gccattatgc accgcggtaa gctggtggcg gtagagcgcg
    17761 gcattcagtt gcgttcgcga gccatccgcg tggttgaaat gcgttttgcc gatagcatct
    17821 caaaagatgg ctttgccgat attccgaaca tccgtaacct gaccattgag ggcaacaagc
    17881 ttcgttgcgt gctgcaggga gaacccgaca ggctgatcaa gacagcctgt cagcatcaca
    17941 tcactgacct gctcagcacc cagcccagcc tggagcaggt gctcgcggat acgtacggga
    18001 taaagaccat ctaacaagct tgttattgat aaccgctcta ttaccagagc caatgagtga
    18061 atatcgcagc ctgctgaaag gttcgatcga ttttaagagg gatgctataa ttcgagttca
    18121 ttcactcatc agcagaggta gacatgcgaa acgaagaagg cctggctttc cacctgcata
    18181 ttcaacaagg cgacattggg cgttacgtgt tgctgcccgg agacccagcg cgctgtgaaa
    18241 agatcgcgcg ttattttaaa gacgctcatt tcgtggcgca gaaccgggag tacgtcacct
    18301 ggaccggcag cctgctgggc gagaaggtct cggtaatttc caccggcatc ggctgcccct
    18361 cagcggccat cgccgtggag gaagccatcg aagccggcgc ggacacgctg ataagagttg
    18421 gctcttctgg ttcgatgcaa cctcatatcc acactggcga tgtggtggtg gtcagcgccg
    18481 ccatccgtga tgaaggcacc acgccgtact acctgcctat cgaattcccg gctgtggcag
    18541 atattgaggt ggtgaacgcg ctgcgcgaag cagccgaaag gctcggctac aaatctcaca
    18601 cgggtatcgc gcaatctaaa gattctttct acggcgaggt agagcgcgcg cgtatgccca
    18661 tgccggctcg cctggcggaa cgctggaacg cctttatcac cggcggcgcc gcctgctctg
    18721 aaatggaatc ttcagcgctt ttcattctct cgtccattta ccgcaagcgt gccggcggtg
    18781 tgatgctggt catcaacgaa tctgacctcg cagaagttga cgaggacaac cccgccaagc
    18841 acatggctga gttcgatgct gaccgcgtca ttaaagtggc ggtcgaagcg ctaaaaatcc
    18901 tcatccagtc tgaccgccgt taggtacctt ccgctttgaa acccacgaca ctgcactcgc
    18961 gcatcaccag cctctcacgt gaactggccc tgttcatcac cgcttctttc gccgtgggca
    19021 tggccgccag cctggtggat tcaacgctca ataattttct gaacgacact ttttcgctga
    19081 acggctttca gcgttctttc ttggagttcc cgcgcgaatt cccgggggtg attgtgctct
    19141 ttgtttcggc ggcattgtgg ttcctgggca gccgccggtt gggcgccttc gccatgctgc
    19201 tggcagccct cggtatattc cttatcggct tttcttcctc aagttacggc ttgatggtga
    19261 tcttcctgtt catctacagc aacggcaatc acctctttat gcctgttgcc actaccattg
    19321 gcatggagct ggctgctgac gggcgcaccg ggcgtcgact ggggcaattg aacgccatcc
    19381 gcaacctggc taccattgcg ggcagcttcc tcgtcttcat cggtttcaaa ttcctggggt
    19441 tcacttacca acatagtttt accatcgccg cattggtctt caccgcggca gcggtgctga
    19501 tgttcttaat gaagccggag aaatccgcgc cgccgaagac cttcctcaag ctgcgcaaag
    19561 aatacacact gttttatgcg ttggctgttt tagcggggtc gcgcaagcaa atctttatca
    19621 cattcgcccc gtgggtcatc gtcactatct tcaataaacc cacccagacc atcgccacgc
    19681 tggtgaccat tggtggggtt atcggcatcc tctttcaacc cttcctgggc aaagcgatcg
    19741 atcgctatgg cgaacggctt atcttgagtg cagaagccct ggtgctaatc tttgtgtgcc
    19801 tggggtacgg attctcgcgc tctgtctttg ctgaaaacgt ggcgtttctc attgtgtgtg
    19861 tttgctacct gctcgatcag atgctttttt cggtcagcat ggcgcgttcc acttacatca
    19921 aaaagatcgc gcttgacccg tcggatatcc agccagcgct ttccgcatcc atcagtatcg
    19981 accatgtttt ttccatcagc gtggccctgc tgggcggcgt catctggaac aactttggct
    20041 ttcagtacgt tttcttgctc ggtatgctca tcgccatggt caatttcatg gtggcgcggc
    20101 gtatcgtcat ccccaaaccc tctataaccg aaaaaccggt cccccagccg atcgagcggg
    20161 attaaagcgc gcagaaccct ctccatcgct caaacgaaat ttggatataa tcaagttaca
    20221 gtatcccaaa aaaaacgctg ggtaagcgtt gactgaaaga agggtaaaaa tgaaagtgaa
    20281 tttttccatg tggaaaaaag ccctttccac cctggtaaag atggacagca aggaggagtg
    20341 ggatgagctg gatgtggttt ccaagtggct gattgctacc cgctccgcgg tgactatcgt
    20401 cactttgtac tcctgtgcta ttggcggttt gttggctatc aggcacggct acttcaacga
    20461 caactgggga atgtccattc tcgcctgggt catcgttacg ctggggttgt tcatcgctca
    20521 cggcacgaac aactttctca acgattacac tgacttcgct cgcggagttg acacggaatc
    20581 ctacttccgt acacagtaca gcgtgcaccc gctcatccaa aagttctgga gcaaagaaca
    20641 acaattccgc tggttcgcca tcagcggcac tctcgccatg ttagccggtc tctttgccct
    20701 cttttacacc cacttctcgc tcgacatcat tattctcttc gctgtgggcg cgctggtgct
    20761 gctgttttat acctggccac ttaagcatat cgctttggga gagctttcca tttttcttat
    20821 ctggggaccg ataatagtgg cagccgtttt cctggtgctg gcgaaaggtt caaccgagca
    20881 tgtgtggccg gtcgcactgg ctgctgtgcc atgggggttg agcgtggtaa gcatcaatct
    20941 cggtaaacac atcgacaaag cgcccgagga taaaaagaaa atggtcggca cgctgccagt
    21001 ggtcattggc gagaaagccg cgcgcattac caatatcatc ctgcttgccc ttatttatct
    21061 ttttattctc tacctggttt tcatcagccg ctatttctct cccatcctgt taattgtgtt
    21121 ctttgccggt caacgcatgc tgtacgccat cggcgtgcat accaagccgc gcccggctga
    21181 gccccctcaa gattggccgt actggccaac ctggttcgcg ggtttcactt tctatcacaa
    21241 ccgcctgttc agtaatctgc tgctgatcgc cattctcatt gacaccttat tacgcttgtt
    21301 tttacctggt ttttggccgg cgcattaaag cgccgcacct ctcctaaaag gagcatgtta
    21361 tggatattgt ccttctttcg cgctggcagt ttgccctcac caccatttat catttcttct
    21421 ttgtgccgct cacgcttggg cttggctggt tcgtcgccat cctcgaaacg atgtacgcgc
    21481 gcagcggcaa cctcttgtat aagcgcctca cgaaattctg gggcaagctc tttctcatta
    21541 attacgccat gggcgtggtc acgggcatcg tgatggagtt tcagttcggt atgaactggt
    21601 cggagtattc acgctttgta ggagatgtat tcggcgctcc attggcggtg gaagggttgt
    21661 tagcgttctt cctggaatcc actttcctgg gggtgtggat ctttggctgg gaacgccttt
    21721 ccaagaagct gcacgccgcg gtgatgtggc tcgtggctat cggcggtaac ctgagcgcgt
    21781 tttggatcct ggcggcaaac ggtttcatgc agcaccctgt gggatacgcc attgtgaacg
    21841 gtcgcgcgca gatgaccgac tttggcgcgc tgctgggcaa cccgcgcgcg ctttacctct
    21901 ttctgcacac cttctttgcc ggtttggtga ccgcttcatt ctttatgttg gggttcagta
    21961 tctaccacct ggtcaagaaa caagaagtgg aggcttttag aaaatccttt gtgctttccg
    22021 ccttgattgg cctgctctcc acagtagcgg tggggggcgc tggacatatt cagggcgttt
    22081 acctgcgtga agtgcagccc atggctgctg ccgcttcaga agcccattat gaaacagcca
    22141 acccggcgga ctttcaccta atcgccggaa ttgaccagga tgggcgcaaa accacctggt
    22201 cagtcaccat tcccagggcg ttaagctttt tgtatttctt taaatgggaa ggcgaggtgg
    22261 aggggctcaa ccaggtgcag gcgcgctatg agcaacttta cggtccagga aattacatcc
    22321 ccaacattgc cctgaccttc tggtctttca gagtgatggt gggggtgggg ttgctgatgg
    22381 gattactcag ccttggcgcg ctggtcatcg cttggaaaaa gtaccccgaa aagtggattg
    22441 agcgctctgg ctggctggtt cggatcattc tcctgcctta tctcgccaac accaccgggt
    22501 gggtgttgac ggagaccggc cggcagccct gggtggtgca tggattgatg caaaccaaag
    22561 acgccgtcag ccctaacctg acacccgcca cagtgctcat cagtttgatc ggttttgtgt
    22621 tgatctacgc ggttttaatg gtgattgacc tttacctgct ggtgaaattc gccaaaaaag
    22681 ggctcggcgc cggagattta gattcggttc ccattgcggg tgaagttgaa gcaaagaaaa
    22741 aaggaggaaa atgatgaacc tcaatatcct gtggtttgtg ctggttgccg ttttgtatgc
    22801 tggtttcttt ttcctcgagg ggtttgattt cggcgtcggc atgctgctgc ccgcgctggg
    22861 gcgtaaggat gtggaacgcc gcgccatcat caacaccatc ggtccgcact gggacggcaa
    22921 cgaagtgtgg ctcatcgtgg cgggcggcgc caccttcgcg gcttttccgc aatggtacgc
    22981 caccctgttc agtggttttt acctgggatt tttcctattg ctcttcgccc tcatcgtgcg
    23041 tggtgtgggg ttcgagtttc gcagcaaact ttccgcacct cgctggcgct atggctggga
    23101 ctgggcgatc gccgtctcca gctttgtggc tgccttcttg ctgggtgtgg tctttaccaa
    23161 cctggtgcgc ggcgtgccga ttaacgctgc catgcaatac accggcggtt tcttcacact
    23221 gctcaaccct atcagcctgt tgggcgggtt gacggtagtg atgctctttc tgttccacgg
    23281 ggcgaacttc ctcaccctta aactgagcga tggactgcgc gagagagcgc gtaacgccac
    23341 gagaacctat ttcaccgccg tgctgttgtt cgcgctgggg ctgtttgctg cgctgctatt
    23401 caccccttcc gccttttcca caaagggcgt tttcagcgct gttctaccag tgctggccgc
    23461 cgcttcgctg gtcgtttcgc gggtaatgat ggggaaaaag aaggaaggct gggcgttcat
    23521 ttgctccagc ctggcggttg tgctcaccgt ggcggcattg ttcaccgcgc tcttccccaa
    23581 cgtgatggtg tccaccacaa acccggcgaa taacctgacg atttataacg cttcctcgtc
    23641 cgattacacg cttaaagtgc tctccatcat cgccgctgtc ttggtgccgg tggtgctggc
    23701 gtatcaaacc tggacctatg tgatcttcag gaagcgcgtg aaagcagaca ccaagacact
    23761 gacgtattaa cctgtctgtt cgcttgagcg caggggcaat ggatcagttg cccctgtttt
    23821 ttattgccag gttcaccgcc gcctcagcgt ggatcttcgt ggtagcgaag attggcaata
    23881 cgctgtcttg cgggcgcacc aggttttcaa tttcagtgca gcccaaaatc accccctgcg
    23941 cccccgcgtc ggctaaattg ctgatcacct tgctgaattt ctccctggac gacgctttca
    24001 ccactcccat gcagagttct tgataaatca cctcgtggat gatctgccgt tcagcttcgc
    24061 ctggaacgag cacgttcagc ccatgttttt tcgccaggcg gtctctgtaa aaatcctgtt
    24121 ccatagtaaa acgcgtgccc aataacccca cctgcgtgat tccagcggct ttcacagcat
    24181 cagccgtggc gtcagcgatg tgcagcagcg ggatgcccag ttcggcttcc aggcttggcg
    24241 ccagtttgtg catggtgttg gtgcataaca ggatgaagtc cgcgcctgcc gcttgcagag
    24301 cgcgtccagc agcaagtaaa caccgcgccg cttcctgcca gttgccctgg cgctgccatt
    24361 cttcgatctc agcaaaatca acggagtaca gcacgcatct ggcggaatgg aaaccgccaa
    24421 gccgtttttt cgtttccagg ttaaggatgc ggtaatactc gagggttgat tcccagctca
    24481 taccgccaat cagaccgatg gtttgcatag gctgtctcct gacgacctct gttacctcat
    24541 tttacatcag catttgtggt tgtatttttc gtaaattttt gtacaataca attacctggt
    24601 aaaaccacca gccaagcatg cgtacaacct ttgaaccgtt gcaagtcacc
//
LOCUS       DAOSPM010000021        24000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2125_length_24000_cov_13.371810, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000021 DAOSPM010000000
VERSION     DAOSPM010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24000
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2125_length_24000_cov_13.371810"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="PLW25_03285"
     CDS             complement(<1..1468)
                     /locus_tag="PLW25_03285"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOS53543.1"
                     /translation="MKSGLAQPMSVLGIEIGDVHTRGHLFDIVENSYRLIASSSAPTT
                     SFYPILDIGDAIYEVLSRIQEICGRTLIEKSGELILPALPTGDGVDQFYITTSCFSSL
                     QVVAVGLLNDVSLDSARKLCNATYANLLESIGINDRRSYSQQLDTLLTIRPDLVIFTG
                     GTDGGASRSLLRTAELVSTALQLLPRGTRPQILYCGNQVLSAQLKDVFERYTTVRSAP
                     NIRPSFEGENLEPALTELDALVMEKMNEEIGGLKRTSPLCTTPPQLKNQGFMRMIKFL
                     GRQYDPLKGVLGLDVGMNYSMAAYSNDRVSILNTFNFGIGSGLEAFLRKGDIKDISRW
                     LTFPVPDTVVSDALWNRSLFPLSVAATKEEIAIEQAVLRQMLQAMMIDLESREIMPSL
                     RFEPIVLSGCTVSHVADPVRSLLLALDGIQPLGISPLILDKHSLLPLLGTIAAHNSLL
                     AVQLLESTAFSNLATVVNITSKTRAGSPLLYARLDYADG"
     gene            complement(1474..2145)
                     /locus_tag="PLW25_03290"
     CDS             complement(1474..2145)
                     /locus_tag="PLW25_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53544.1"
                     /translation="MSFNTEVIWVVISFILTLVVFSFLLGDIPFFRFVSAVLIGMTAG
                     YMTIVIIYQVLIARLAVPLLQGSRVALIPLLLSGMLLMKLSPRLSRLGNPSMALLVGV
                     GAAVAVGGAVLGTLFGQVKGALVFVDLARAQKGPTSLLLLVEGIFFLLGTLATLFYFH
                     FSARKVKGKEPQAHWSIRLLSSVGRVFIAITLGAVFAGVITAGLTALVERADFLLGTL
                     LFWVR"
     gene            complement(2145..3815)
                     /locus_tag="PLW25_03295"
     CDS             complement(2145..3815)
                     /locus_tag="PLW25_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53545.1"
                     /translation="MAEISGRVEPTEESGESWEGEAPEWLARIRMLKEQDEQKTRETS
                     GLDTEDSGLPEWIINLRAAEPPVMAEKTAEEALLEDSDAKATTPADTYADSRFEETGG
                     RASGEFTGEPVEGETTSPAANKPFETAELRDGQPAHPIFEDDLPEWLSADAGMVETHS
                     SQKDQTARPFISKEENKLADGVLPAWLKAIRPLDAVSPATPDALYSPGEEGEGILAGI
                     SGTLRSQRLEDREVKMPSRPHELVVTPMQQKNAELLYRLSNPDIEETSPVAITKKSRP
                     QRKTLKIFAAVILFLAVLLPYGFRSLPVIIPVLFPKEVVDTLGVIEGIPAGKPVLIAA
                     QFEGGLAGELTLSSEPVFQHLVSHGVPLALMSTNASGYAILEEQLRRASAGVSGYLFS
                     ERVVNLGYLPGGTIGLISLVGDIRAALPYSTALEPAWETTPIAGARTLADFAAILLLT
                     DNPEIVRAWVEQTGRIEKPPILLGVVSAQAAPLAQPYYDSGQVQGLVAGSSGALAYQM
                     IRQVPGKASTTFASYQFALFIAALLIFLGGVVSLVTTTGAAAHKKGRG"
     gene            complement(3965..4582)
                     /locus_tag="PLW25_03300"
     CDS             complement(3965..4582)
                     /locus_tag="PLW25_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53546.1"
                     /translation="MKVLNYLIAAITGLIVLLGYFFPGTGISLARSPLLDWAVTLSGV
                     AGLVAILNLVFHVHWKRIQDKHPRRLYSGALIVTFLLTVVAGVFLDPGSAGFQKLVTR
                     VQVPVESTLMAVLAITLVFASLKLLQRQRNWMGFLFFLSVIIFLVLNSGVLAFSTEIP
                     ILTNLLSAFHRVPMAGARGMLLGIALGSLATGIRVLIGSEKPYNG"
     gene            complement(4579..5331)
                     /locus_tag="PLW25_03305"
     CDS             complement(4579..5331)
                     /locus_tag="PLW25_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53547.1"
                     /translation="MKAEPILGLLVVIFAAGWMIFLSYRKKSSEGRATFRRITAINRL
                     RSAVGLSVEDGSRVHISLGNGDLNEASNPSGLAGVDTLQRIGQLSSASDQPPVCTSGN
                     GGFTLLSKDVLRMVAGETQSTEMFDADHGRMAGATPFAYVLGTLEVMNEPAVRTNVFM
                     GSYGAEVGFLTTAAEENGAFTLAASDSIVAQSVFLATAREVMLGEELYAVPAYLVHRS
                     SHVASLKTQDLLRLIISGALLLGLLLKLAGIA"
     gene            complement(5328..5507)
                     /locus_tag="PLW25_03310"
     CDS             complement(5328..5507)
                     /locus_tag="PLW25_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53548.1"
                     /translation="MMLSLLEDGGIFRHFISQAMLAVAPFFNHSAASWQAFADMLEDP
                     AIALSFAEHLREEDA"
     gene            5719..6027
                     /locus_tag="PLW25_03315"
     CDS             5719..6027
                     /locus_tag="PLW25_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53549.1"
                     /translation="MPFFFTWFKPRPYSAANSREVHSLLEELIRIGIKEDYLSEIPGY
                     GYNSQCRHIRTREIGKRLHELGGNELMSWAFARVRKQAGKVPASHLEYAWNDIGGWQP
                     "
     gene            complement(6051..7190)
                     /locus_tag="PLW25_03320"
     CDS             complement(6051..7190)
                     /locus_tag="PLW25_03320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS53550.1"
                     /translation="MKIACISTAQVPSTRAHSIQVMKVTQALAQNGEEVVLYLPGKEP
                     PAWQELADQYGLIEPFEIITLPTRPGLKRLDFSLAGVKSARRWGAQLVYTRMLWVALF
                     AGLRAMPFVLEMHDLPSGTFGPHIYRRILRMKAPKLVVYITSALKTLADQRFGVAARV
                     GEYLIAPDGVDLERYSDLPKPAAARKKLALEEAMTAGYSGGFYPGRGLELMQQLARAF
                     PQVRFLWIGGNDDQVEWWRARLKEADIHNVQLTGFIPNSQLPLYQAAADILVMPYSRA
                     FGGSGGGDISAVSSPMKLFEYMAAGRCILASDLPVLREVLNDQTAAFFEIENFSDLRE
                     KFAELLTDGTRREKLASAAKSAVNAYSWKTRMTGIMRVMNNTLNH"
     gene            complement(7192..8544)
                     /locus_tag="PLW25_03325"
     CDS             complement(7192..8544)
                     /locus_tag="PLW25_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOS53551.1"
                     /translation="MNSLFARIRKLLVEDHILTRVLKNTGYLFSSTSFGLLLSLVQSV
                     FAARLLGVAAFGLVGIITSFVSNVNRVFSFRMGEFIIRYLGKELTENNRQKAGAVVKV
                     AMLTEGFTSVVAFFVLLLLAPLGAKYIAKDMQALPMIQLFAVSILANMVSETSIGVLQ
                     ITNHFRTQAMINVIQSVVTALLILLAYLLHGGIIFVLSAYLVGKILLGISPAILAFYY
                     LPRHLQPGWWKTPLSLLPPFKELAHFTLSTNLSGTVKMIASESEPLWVGFFLDQQSVG
                     LYKLALSIVNPLMMPITPFVTTTYPEMTRSIVSRAWAQLRRLLRRVTLISASWTGLVL
                     LVMLIAGRWLIQLFYGAAFVPAFPATMVLLLGFGFANVFFWNRSLLLSFGQANIPLYV
                     LFSAALVKIALAFWVVPRWGIVGESALLSAYLIISTALLVFIGLRKARQGEKADLKGE
                     "
     gene            complement(8541..9518)
                     /locus_tag="PLW25_03330"
     CDS             complement(8541..9518)
                     /locus_tag="PLW25_03330"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOS53552.1"
                     /translation="MARYLVTGAAGFIARRVIEQLIAQGHEVVGVDNLNDSYDPRLKE
                     WRLQQLARLPGFRFFRDDICRPEFYQSLSGEFTSFQGVIHLAARAGVRQAVEIPQVFL
                     QTNAAGTLNLLEWCRRSGTPKLVLASTSSIYGANPPLPTPENADSCHPLQIYAASKKA
                     AEVMAYTYHYLYGLDTTVVRFFTVYGPAGRPDMVMYRFTRWIVENEPVCVSGDGNQMR
                     GFTYLDDIADGVIKALKPLGYEIVNLGGHETITINELLQRIEHLVGKKANVHYIPGHP
                     ADVNANWADVTKARSLLGWEPKVLLAEGIPQLVNWYLEQRAWARDIPLP"
     assembly_gap    9647..9746
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9747..10684)
                     /locus_tag="PLW25_03335"
     CDS             complement(<9747..10684)
                     /locus_tag="PLW25_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOS53553.1"
                     /translation="MDYAQELIEKFKKKTAKVAVLGMGYVGLPFSTVFAQAGFAVTGI
                     DPVEQKMEMLNRGESYILDVPSLVVRQLVADGKLRGTTDYAILKEIDAVAICVPTPLR
                     HTGDPDLSFIISAAEGIAPYVHPGMVVVLESSTYPGTTRELVLPALTEKSGLKIGEEL
                     FLAFSPERVDPGREDWTTKNTPKVIGGMTEKCTEVSTAWYSMALDTVVPVSSTEVAEM
                     AKLLENTFRMINIGFVNELTLMCDRLGVDVWEVIDAAATKPFGFMRFNPGPGLGGHCI
                     PIDPMYLSWKMRSLNYTARFIELASEINTNMPRFVIG"
     gene            complement(10701..11351)
                     /locus_tag="PLW25_03340"
     CDS             complement(10701..11351)
                     /locus_tag="PLW25_03340"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOS53554.1"
                     /translation="MDTAEILREIAAQVATCQACTLYHSRKNAVPGEGSPTSEIMFIG
                     EGPGFHENEQGRPFVGQAGKFLDELLGEAGYTREQVFIANVVKCRPPNNRDPLPDELA
                     ACARFLDRQIETINPHVIVTLGRFSMAKFLPNARISEVHAKTYWVRGRLIVPMYHPAA
                     ALHQPSLKSTLINDFRTLPAAIASARAAAKRQPAQARTSAEGVKEIEEKPEQLSLF"
     gene            complement(11402..11911)
                     /gene="rimI"
                     /locus_tag="PLW25_03345"
     CDS             complement(11402..11911)
                     /gene="rimI"
                     /locus_tag="PLW25_03345"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOS53555.1"
                     /translation="MSETEVSGTLRIRRMQSEDLPQVFELDRSSFSLPWPERSFRFEL
                     ENNEVSRCWVAELLSESGAPLLVAIIVVWMIADEVHVATLAVSPAYRRQKIAQRLLAH
                     TLIDAYHSGANSSFLEVRRSNQAAITLYQRFGYREVAVRRNYYKDNQEDAILMDLAPL
                     NLENLKRFQ"
     gene            complement(11908..12576)
                     /gene="tsaB"
                     /locus_tag="PLW25_03350"
     CDS             complement(11908..12576)
                     /gene="tsaB"
                     /locus_tag="PLW25_03350"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOS53556.1"
                     /translation="MLLAIDTSTQTSGIALYDEFVLIGEMIWRTASHHTVELAPAVQD
                     LMNRCMVSPAELKAVAVALGPGSFTSLRIGLALAKGLALSLNIPLLGIPTFEFQAAQQ
                     PLAAEPLLTVLPAGRSRLAVQEFSSSSGCWQPQDEARVMTAEEISDRISGPTRICGEM
                     SPSERQVIGRKWKNAMIAPPALAMRRPSMLAELAWKRWNEGEVDDPALLAPIYLHVAG
                     MIQE"
     gene            complement(12577..13071)
                     /gene="tsaE"
                     /locus_tag="PLW25_03355"
     CDS             complement(12577..13071)
                     /gene="tsaE"
                     /locus_tag="PLW25_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOS53557.1"
                     /translation="MPILPAYSIDFVSRSPEQTRRLGMRMGTLLKSGDLLCLEGDLGS
                     GKTTFVQGLAHGWGSVDAVTSPTFVIVNQYRRAGGGLMHHLDAYRLTSIDEAIDLDLD
                     DMLSGGILVAEWAERIESVLPADRLVIKYAWLNEEQRNLLFIPVGERYKKMLDDFKRL
                     AFGG"
     gene            complement(13101..13397)
                     /locus_tag="PLW25_03360"
     CDS             complement(13101..13397)
                     /locus_tag="PLW25_03360"
                     /inference="COORDINATES: protein motif:HMM:NF017879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOS53558.1"
                     /translation="MGNFLLSPASCGEFENYNMGYNRSMLPELKLGDRVRLRKPHPCG
                     GYTWKIVRLGADIGLECDTCSHRVMLTRRELTRRMKTYLPIDAPQENPPSDVYQ"
     gene            13398..14048
                     /locus_tag="PLW25_03365"
     CDS             13398..14048
                     /locus_tag="PLW25_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOS53559.1"
                     /translation="MLNLAPSLLFSRILTLIIALTIHEFSHALVADHFGDVTARNAGR
                     LTLNPLKHLDVFGSLMLLVVGFGWAKPVPVNPYALRRRSESALMWVSLAGPASNLLLA
                     ILAGLVLRFRMVPWRASAAGSMLPSPAEFLFTFLSINLLLMLFNLIPLSPLDGEKVLS
                     YLLPYRLQPGFESIQRYGPLLLMVLLFVLPLVGIDLIRWVMSPALTQLQKFIIGVG"
     gene            14048..15022
                     /locus_tag="PLW25_03370"
     CDS             14048..15022
                     /locus_tag="PLW25_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOS53560.1"
                     /translation="MSPQIALIGLNQVGASIGLALGAYKENITRVGHDADSARMRRLE
                     KEGVCDKTHTRLPDAVRGADMVILSLPTDLLRDALGLIAGEVFPQAVVVCISAVARVA
                     YQWARELLPPQQPFIVLQPVLHPARLRDWEENSSNPYADLFENTHMLVVTDSNTQEKA
                     FQLANDLCALLKAKPYFSEPLEADGITARIEQLPKLTAVALLSMLVEKPGWDDARKMT
                     NRAFFRFASLNTLFDEQEFFGVSNLVNRENITRALDELSATLAEIRQMIDAGDEEGLQ
                     KMYQTARQGYETWLTQRMSGEWDKVEKPPEALERGILTRLFGGRPVKK"
     gene            15032..15307
                     /locus_tag="PLW25_03375"
     CDS             15032..15307
                     /locus_tag="PLW25_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOS53561.1"
                     /translation="MSFYCYIVECADGSYYTGWTLDPARRERQHNLGKGAAYTRVHRP
                     VRLVYIEEQPDRSTAMKRERAIKALNHTQKARLISKFTEMGDDRGDP"
     gene            15294..16427
                     /gene="galK"
                     /locus_tag="PLW25_03380"
     CDS             15294..16427
                     /gene="galK"
                     /locus_tag="PLW25_03380"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOS53562.1"
                     /translation="MATPEGTVISPGRVNLLGEHVDYNDGVVLPAAIDRSVKIIFKKN
                     DSRLVSLKAVDLQDQCVIHLSHLEKKTSQEGKPLPDWALYPAGVMWSLQRHGLDLIGF
                     DAQFSSNIPIGAGLSSSAAIEVGFAVVCMSLCGWQLERLELAKLCQQAEREYVGVNCG
                     LMDQFACACGVEDHVVMFDTRSLASQTLPLPPATTLVIADSSLRRSLLTSAYNDRRRD
                     CESVVAYFNQLDPSIRSLRDLGEERLLAHEHALPPAAFRHARHVVEEISRVNQAIDLL
                     KEKDARGFGRLMFSSHDSLRDLFEVSRPELDILVDIAAGFDGCLGSRLTGAGFGGSTV
                     SLVLDEKSDAFIHQLRDLYQKQTGLQAVVFSTRASRGAHLNPD"
     gene            16447..17442
                     /locus_tag="PLW25_03385"
     CDS             16447..17442
                     /locus_tag="PLW25_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53563.1"
                     /translation="MLTSTNDRAIETHSLVKTFHTKHGFLTRTTRSTEAVRGITFHVN
                     KGELFGLLGPNGAGKTTTVKMLSTLLIPTSGSIHIFGQDILQHTRDIRKHIGFTFGGA
                     HGLYGRLTAIENLRYFAELYALPPRETHNRITELLELVGLRDRGNDRVETYSSGMQQR
                     LHLARVLLHDPELIFLDEPTVGIDPVGARELRSTIKLLKNQGKTILLTTHYMAEADEL
                     CDRLAIINHGQIIAMDSPSSLKSKLTSDAFVELKTSGENLPSVKNVLAQLDPDSEVTY
                     NQHAFVWEVNIRTRQPGVVMELLAPLLSSNLIQTLGVRNQTMEDVYVSIIQNGGA"
     gene            17439..18230
                     /locus_tag="PLW25_03390"
     CDS             17439..18230
                     /locus_tag="PLW25_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS53564.1"
                     /translation="MIGNFLRLGIRQMNVQLRMRVLSLWSLALFFTQPAIFSAIGMLL
                     ARAVGNTRPDLVYTVLGAGIMGMWSGLVFTSTYDIRADRREGTLELIVGSPTPLRRVV
                     GFRSLLNILAGSTSMLAAVAAALFIFDYSFSGINIPGVVISFSLLLLALWCMGIFLAN
                     ILAWSRLSGTFVDLLEMPIALLCGFMYPISVLPAWMQQISAVFPIRWGLETLQDALKG
                     SLIDSALVNKWLIALGVSFVYYLLARWFDRKVHDHIRITGELHSI"
     gene            18227..19027
                     /locus_tag="PLW25_03395"
     CDS             18227..19027
                     /locus_tag="PLW25_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS53565.1"
                     /translation="MIKRLGHLFRLYFFQAWFSYRSLFAWSTPFSYATSKFGFPFFAM
                     LFFVFMGKFVGFLDPMFIVIGNILMMPTTNGLYGISMTIGNERQFGAMSYLLGSPAPR
                     APLFLGRAFFHILDGFVTVLVALPIAMLIFHIDFAEVNLFMLACCLVLLSITTTGLGF
                     IMGTVSLLSRDGWMFTNTLGQLLYILIGVNIPIERLPGFLQPLSGYLPMTRGILATRL
                     ALGGAGWSEVASLLAGEILVAAVYMVFGYTLFRLVERASLSTGSLDSI"
     gene            complement(19056..19607)
                     /locus_tag="PLW25_03400"
     CDS             complement(19056..19607)
                     /locus_tag="PLW25_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOS53566.1"
                     /translation="MKKRETYPIEIAGVHRDLTLFEIKPGLKIAILNILGDMELVQSC
                     AAALAEKLTAVDYDLLLTAEAKSIPIAHALAAETMKPYVVLRKVYKPYMGDALKAETL
                     SITTGEPQYLYLDEKDRELMMGKKVVIVDDVISTGSTLQGMRLLVQKAGAEVVAEAAI
                     LTEGERARWHEIISLGHLPVFTD"
     gene            20096..20437
                     /locus_tag="PLW25_03405"
     CDS             20096..20437
                     /locus_tag="PLW25_03405"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOS53567.1"
                     /translation="MGEYFAGQRKAFDLPLDWQSIRGFQREALLELLKTPYGEVRTYG
                     EISRALGKPAAARAVGMALAKNPLPILIPCHRVVAASGHLTGFSAGDGLNSKAWLLQL
                     EGHRVVTEKLA"
     gene            20415..21326
                     /locus_tag="PLW25_03410"
     CDS             20415..21326
                     /locus_tag="PLW25_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOS53568.1"
                     /translation="MSLKSWLKFILLGIAWGSSFFWIKIGLQEVGPFTVVFFRIFFAL
                     IGLTVFFLLNRRKPLLKAWRLYLFLGVFYVALPFALVAWSEKYITSGLASILNSMQPL
                     ATSLIAAVFIQEERLNARRIVGLLLGFGGVLVLMSDKLSGEAINQIYGIIAMVAAVFC
                     YGGSSVFARMHNQGVKPEDQAIGQFVFGILFIFPTMLAVESPFIMPVRPVSYLAFAWL
                     GLIGSFFAAIAWYSLLDEIGPSRVSMTTYLFPLVGVLLGAILLHEAVDWRVIVGGLMI
                     LGGIVIVNSGKRNNQTPAQVQADPFEG"
     gene            21328..22242
                     /gene="era"
                     /locus_tag="PLW25_03415"
     CDS             21328..22242
                     /gene="era"
                     /locus_tag="PLW25_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOS53569.1"
                     /translation="MMEKQEFRAGYVAVVGRPNVGKSTLINALLGQKIAAVSPKPQTT
                     RRNQLGILSLPEAQLIFVDTPGLHDPQHKLGDFMNQAAGEALRDADILLWLLDASAPF
                     TSEDHLIAEKLGGMRSLPPVWMGLTKTDLVNEKTLEERKRQAVELYPAQDVLTLSVKN
                     KASLDALLEVMISRLPLGEAFYESDTVTDLYERDIAAELIRESVLTFVQEEVPHCVAV
                     KIDAYEERPGEKAYIEATLFVERDSQKGILIGKGGEMIKKIGTHSRQEIEEMSGCKVF
                     LELKVRVNKNWRNSPDALELLGYKQVAG"
     gene            22246..22917
                     /locus_tag="PLW25_03420"
     CDS             22246..22917
                     /locus_tag="PLW25_03420"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOS53570.1"
                     /translation="MLTWAFWLAGFFALLDWAAVWKGWRKRLYLAKPAVMVFLIFWTL
                     QLTGWRNEMTWFGAGLVFSLIGDIALMLNPRFFMVGGTSFLFAQVAYFIGFNTSPAPL
                     SGGVFLVAVLVGAAAAVIFKRIRPGILRVPRGRRFLAALCLYGLTLTLMLLSALLTLF
                     RPEWQYQPAILAAIGALLFFSSDTMLVYDRFVRRFNHAQSYVHLTYHLAQFGIISGAV
                     LHCLR"
     gene            complement(22923..23840)
                     /locus_tag="PLW25_03425"
     CDS             complement(22923..23840)
                     /locus_tag="PLW25_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOS53571.1"
                     /translation="MTAQKGTVTALWPRFTSAIGPLEFTWKTAREYLVVLLGALIQAL
                     AMRLFLVPGQLVSGGISGAAQIINHFAPIPIGLMVFIGNVPLFFLGWRFLGGPRFALR
                     TAIAVGAFSLFTDLLVFIIPAQGVTRDNVLNAVFGAVLLGVGLGLVYRGGGTSGGSDI
                     LGRILNHRLGISISTAYLITDSIVVLAGGFAFSWEKALYGMVVIYISGLAAEMISEGS
                     GIFRTAIIISPYHEKISSEIMSVLSRGVTVLSGKGAYTGTDKPVLYCVITRAEVNQLK
                     QVVKEIDPQAFMVIGVTHEALGEGFRPLK"
BASE COUNT         5522 a         6662 c         6318 g         5398 t
ORIGIN      
        1 tgccatccgc gtaatccagc cgcgcgtaaa gcagcgggct gcccgcgcgc gttttgctgg
       61 taatattgac aactgtcgcc agattggaaa aagcggttga ttccagcagc tgcactgcca
      121 ggagcgagtt gtgagcagcg atcgtcccta ataagggtaa aaggctgtgc ttatcgagga
      181 tcaaggggct gatgcccagg ggttggatgc catcaagtgc cagcaacaaa gaacgcaccg
      241 ggtcggccac atggctgacg gtgcaaccac tcagcacaat gggctcaaac cgtaaagatg
      301 gcattatttc ccgcgattcc aggtcaatca tcatcgcctg taacatctgc cttaataccg
      361 cctgttcaat ggcgatttct tctttggtcg cagccacaga aagggggaat aaagagcgat
      421 tccaaagggc atcgcttacc actgtgtcag gcacaggaaa ggtcaaccag cgggagatat
      481 ctttgatatc gccttttcgc aggaatgctt caagaccgct gcctatgcca aagttgaacg
      541 tattaaggat agacacccgg tcattactgt aggcagccat agaatagttc atgccgacat
      601 ccaggcctaa aacccctttc agagggtcgt actgccgacc cagaaacttg atcatccgca
      661 tgaatccctg gttcttcaac tgaggtgggg ttgtgcagag aggggatgtg cgtttgagcc
      721 ctcctatctc ttcattcatc ttttccatga ccaacgcatc cagttcagtc aaggcaggtt
      781 caagattctc cccttcaaag gagggccgaa tgttcggtgc gctgcgtacg gttgtataac
      841 gctcaaacac atccttcagc tgagcgctca acacctggtt tccgcagtaa agaatctgcg
      901 gacgggtgcc gcgcggaagc aattgcagcg cagtgctgac aagttctgct gttcgcaaga
      961 gagagcggga tgcgccgcca tccgttcctc ccgtgaagat gaccagatcc ggcctgatag
     1021 tcagcagcgt atctaactgc tgagagtaag accgcctgtc attgatcccg atggattcca
     1081 acaggttagc gtatgtggcg ttacaaagtt tcctggctga atcgagagac acatcgttca
     1141 gtaatccgac tgccaccacc tggagcgatg agaaacaaga ggtggtgata taaaattggt
     1201 cgaccccatc gcccgtgggc agcgcgggta aaatcagctc gccacttttt tcaatcaatg
     1261 tacgcccgca aatctcctgg attcggctca agacttcgta aatagcgtct ccaatgtcca
     1321 gaatggggta aaaagacgtg gtcggcgcag aacttgaagc aatcagccga taactgtttt
     1381 caacaatatc gaacaggtgt ccgcgcgtat gaacgtcgcc gatttcaatc cctaaaactg
     1441 acattggttg cgcaagcccg cttttcattt tttctatctc acccaaaaca acagtgttcc
     1501 gagaagaaaa tcagcccgtt caacaagcgc agtaagtccg gcggttatca caccggcgaa
     1561 caccgcccca agcgtgatgg caatgaacac cctgccaacg gatgacagca gcctgatgct
     1621 ccaatgcgcc tgtggttctt tgcctttcac ttttcgcgcc gaaaaatgaa aataaaacaa
     1681 agtcgccagg gtgcccagca ggaaaaagat gccctcgacg agcaataaca ggctcgtcgg
     1741 tcctttttga gccctggcga gatcaacaaa aaccaatgcc ccttttacct gcccaaaaag
     1801 ggtgccaagc actgctccgc ctaccgccac cgccgcccca acccctacca gcaatgccat
     1861 agaggggttc cccagccggc tcaggcgggg cgagagcttc atgagtaaca tgccggaaag
     1921 caacaaaggt atcaacgcta ctcgagaacc ttgcagcaat ggcaccgcga ggcgcgcgat
     1981 cagcacctgg taaatgatca caattgtcat ataccctgcg gtcatgccaa tcagcacggc
     2041 agaaacaaat ctgaagaagg gaatatctcc caataagaaa ctaaacacga ccagcgtcag
     2101 gatgaagctg atgaccaccc agataacctc tgtgttgaag gacatcaacc cctccctttc
     2161 ttatgcgcgg cggcaccggt tgtggtgacg agactgacaa cccctcccag gaagatcagc
     2221 aacgcggcaa tgaaaagcgc aaattggtac gaagcgaagg ttgtggaggc tttaccgggc
     2281 acctggcgta tcatctgata ggcgagcgcg ccggaagaac ccgccaccaa cccttgaacc
     2341 tgtccgctat cataataagg ttgagcgagt ggtgcggcct gcgcgctcac cacaccaagc
     2401 aggattggtg gtttttcaat tcgtccggtt tgttccaccc aggcgcgaac gatttcagga
     2461 ttatcggtaa gcagcaagat cgcggcgaaa tcagccaggg ttcgcgcgcc agcgatgggg
     2521 gtggtctccc aggcgggttc aagagcagtt gaataaggca gcgccgctcg aatatcgcca
     2581 accaatgaaa tcagcccaat cgttccgcca ggcaaatagc caagattgac cacccgctca
     2641 gaaaacaggt agcctgagac gcccgccgaa gcgcgccgca actgttcttc gaggatggca
     2701 tatccactgg cgttggtgga catcagcgcg agcggaacgc cgtgggaaac cagatgctgg
     2761 aacactggtt cagagctcaa ggtaagttcg cccgccagcc ctccttcaaa ctgcgcggct
     2821 attaataccg gctttccagc ggggattccc tcgatgacgc caagcgtgtc caccacctct
     2881 ttagggaata aaactgggat gataacaggc agcgagcgaa aaccataggg gaggagcacc
     2941 gccaagaata aaatgaccgc cgcaaagatc ttgagggttt tcctttgagg acggcttttt
     3001 tttgttatgg ctacagggga ggtttcctca atatctgggt tactcagccg gtaaagcaac
     3061 tcagcgttct tctgctgcat gggggtcacg actaattcat gggggcggga aggcattttt
     3121 acctccctgt cttccagccg ctgcgagcgc agtgtgccgg aaatacccgc gaggatacct
     3181 tcgccctctt caccaggaga ataaagcgca tctggcgtag ccggcgatac ggcgtcaaga
     3241 gggcggatgg ctttcaacca ggcgggcagc acgccatccg caagcttgtt ttcttccttg
     3301 gagataaagg gtctggctgt ttggtctttc tgagaagaat gtgtttccac catgcccgca
     3361 tcagcagaca accattcagg cagatcatcc tcgaagattg gatgagccgg ttgaccatcg
     3421 cgcagctcag cggtttcaaa cggtttattc gccgcgggtg aagtggtttc accctctacc
     3481 ggttcacccg taaattcgcc actcgcccgg ccaccagttt cttcgaaccg tgaatcagca
     3541 taagtatctg ccggggttgt tgccttcgcg tcagaatcct ccagcaaggc ttcctcggct
     3601 gtcttttctg ccattactgg cggttcagca gcgcgcaggt ttataatcca ttctggcagc
     3661 ccggagtctt cggtatccaa cccagaggtt tcacgggttt tttgctcatc ttgctctttc
     3721 agcatccgga tgcgggccag ccattccggc gcttccccct cccaactttc tccgctttct
     3781 tcagtgggtt caaccctgcc agaaatttca gccaatggtt ccggttcagg gatctcacct
     3841 tcacctggtt catccggagc aggtggttct ttatagcctg ggagtggtga acgacaatac
     3901 caacagcgtg gtgagctggc atcgttcatt cgattacaaa taaggcagcg aatatccgcc
     3961 ataatcaacc gttatagggc ttttctgagc caatcaaaac gcgaattccc gttgccaggc
     4021 taccaagagc gatgcccagc agcatccctc tggctccggc catcggaacg cgatgaaagg
     4081 ctgagagcag attggtaagg atggggatct ccgtagaaaa ggccagcact ccgctgttta
     4141 gtaccagaaa tatgatcacg cttaaaaaga agagaaaacc catccagttg cgttgacgtt
     4201 gtaacaactt cagactggcg aaaaccagcg tgatcgccag cactgccatc agtgtggatt
     4261 caacaggaac ctgaacccgg gtgacgagct tttgaaaacc agcgctgcca gggtcgagaa
     4321 agacccccgc aaccactgtc agcaggaagg tcacaatcaa tgccccgctg tacagtctgc
     4381 gcgggtgctt gtcctgaatc cgcttccagt ggacatgaaa caccaggttt aaaatcgcca
     4441 ctaacccggc cacgccgcta agggtgactg cccaatccag cagtggagag cgggccagtg
     4501 aaattcctgt gccaggaaag aaatacccca ataacacgat cagaccagta attgcggcga
     4561 tcaggtaatt cagtactttc acgcgatacc tgccagtttg agcagaagac ctaaaagcaa
     4621 tgctccgcta atgatcaggc gcagcaggtc ctgcgttttt aggctggcca cgtgtgaaga
     4681 gcggtgaacc aggtaagccg gaacggcgta cagctcttcc ccaagcatga cctcacgggc
     4741 agtggccaag aaaactgact gcgcgacaat tgagtcactg gctgccagtg taaatgcccc
     4801 attttcctca gccgctgtgg tcaaaaaacc gacctcagcc ccataactgc ccataaacac
     4861 attggtcctg acggcgggtt cattcattac ctccagggtg cccagcacat aagcgaacgg
     4921 cgtggcgccc gccattcgcc catgatctgc atcaaacatc tcagtagatt gggtttctcc
     4981 agcaaccatg cgcaacacat ctttgctgag caaagtgaac ccgccattac cgctggtgca
     5041 aacaggaggt tggtcgctgg cggaacttaa ttgaccgata cgctgcagcg tatctactcc
     5101 ggccagccca gaagggttgc tggcttcgtt gagatcgccg ttccccagtg atatgtgcac
     5161 ccggctgcca tcttccacag aaagccccac cgcactcctc aagcgattga tggcagtgat
     5221 acggcgaaag gtcgcccgtc cttcggatga tttcttccgg taagagagaa aaatcatcca
     5281 tccggcggcg aaaatcacca ccagtaaccc caggataggt tcagccttca cgcgtcctcc
     5341 tcgcgcaggt gctccgcgaa ggatagcgca atggccgggt cttccagcat gtcggcgaac
     5401 gcctgccagg aagcagcact gtggttgaag aatggcgcaa ccgctagcat tgcctgcgag
     5461 atgaagtggc gaaatattcc tccatcttca agcaggctta acatcaaagg tgtcagcccg
     5521 tgacggcgaa acgtatcttg ccattgtgac cagaatcgag gatccatcgc taacaataga
     5581 gtatagcccc tttcatggct gggtcatttt ttagatgcag gattcaggcc agattttcgt
     5641 tcaagcgaca gcgcttcaaa aaaagttgta caattaatgt tgacggaaag cttaagactc
     5701 attcttcagg agcctgctat gccctttttc ttcacctggt tcaagccccg tccttattct
     5761 gcggcgaaca gccgggaagt gcattctctt ctggaagaat tgatccgtat tgggattaag
     5821 gaagattacc tttcagaaat acccggatac ggttacaaca gccagtgccg gcacatccgc
     5881 acccgtgaaa ttggaaaaag actgcatgag cttggcggaa acgagttgat gtcatgggca
     5941 ttcgccaggg tgcgcaagca agccggcaaa gtaccggctt ctcacctcga gtacgcctgg
     6001 aacgatatcg gcggctggca gccctaaaca gaccgtcagc ccaatcgtaa tcagtgattg
     6061 agggtgttgt tcatcacccg catgatgccc gtcatgcggg ttttccagct gtaagcgttc
     6121 acggcgctct tcgccgcgct ggctaatttc tcccgccggg tgccatccgt gagcaattcc
     6181 gcgaacttct cgcgcaggtc agaaaaattc tcgatttcga agaaagccgc ggtctggtca
     6241 tttaaaacct cacgcaatac cggcaggtcg ctggcaagaa tgcaacgtcc cgcggccatg
     6301 tactcaaaga gcttcattgg gctgctgacc gctgaaatat caccgccacc gctaccgccg
     6361 aaagcccgac tgtatggcat caccaagata tccgcggccg cctggtagag gggtaactgg
     6421 ctgttaggga tgaaaccggt gagttgcacg ttatgaatat cagcctcttt caaccttgcg
     6481 cgccaccact ccacctggtc atcatttcca ccaatccata agaaacgcac ctgcgggaaa
     6541 gcgcgcgcca gttgttgcat cagctcgagc ccgcgcccag ggtaaaaccc accggaatag
     6601 ccggccgtca tcgcttcttc gagggctaac ttttttcgcg cagccgccgg tttgggcagg
     6661 tcagaatacc gctcaagatc cactccgtca ggagcaataa ggtattcacc aaccctggca
     6721 gccaccccaa agcgttgatc agcaagtgtt tttaacgccg aggtaatgta aacgaccagt
     6781 tttggggctt tcatccgcag gatgcgccga taaatatgcg ggccgaaagt tcccgacggg
     6841 aggtcgtgca tctccaaaac gaaaggcatc gcccgtaacc cggcgaacaa agccacccat
     6901 aacatacgcg tgtacaccag ctgcgcgccc caacgtcgcg ctgatttgac ccctgccagg
     6961 gagaagtcca gccgtttcaa cccggggcgg gtcggcagcg tgatgatttc gaagggttct
     7021 atcaatccat actggtcagc gagttcctgc caggcaggag gttcctttcc cgggaggtaa
     7081 agaaccactt cctcgccatt ttgcgccagc gcctgtgtca ccttcatcac ctggatgctg
     7141 tgggcgcggg tagacgggac ctgcgcggtg gagatacagg cgatcttcat ctcattctcc
     7201 tttgagatcc gctttttcac cctgcctggc ttttcgtaaa ccgatgaaca ccagaagagc
     7261 agtggaaatg atcaggtaag cagataagag agcgctttcg ccgacgatcc cccagcgcgg
     7321 caccacccaa aaagccaatg cgatcttaac caacgccgcg ctgaacaaga catagagcgg
     7381 gatgttggct tgtccgaaag acagcagcaa ggagcggttc cagaagaaga cattggcgaa
     7441 gccaaagccc agcagcagaa ccatcgtcgc cgggaatgcg gggacaaacg cagcgccata
     7501 gaacagctgg atcaaccacc tgcctgcaat taacatcacc agcagtacca acccggtcca
     7561 tgaagcggaa atcagggtaa cgcgcctgag cagcctgcgc aactgcgccc atgcccgtga
     7621 gacaatactg cgggtcatct cggggtaagt cgtggtaaca aatggggtga tgggcatcat
     7681 taaagggttg acgatagaaa gagccagttt atacagccct acagactgct gatccaggaa
     7741 aaacccgacc cacagcggtt cgctttcact ggcaatcatt ttaactgtgc cgctgagatt
     7801 ggtgctaagg gtgaaatgag ccagttcttt aaatgggggc agtagcgaga gaggggtttt
     7861 ccaccatcca ggctgtaaat ggcggggcag ataatagaaa gcgaggatag ccgggctgat
     7921 acccagcagg atcttcccta ccaggtaagc gctgagcacg aagatgattc cgccatgcag
     7981 caagtaagcc agcagaatga gcagcgctgt gaccacactt tgaatcacat tgatcatggc
     8041 ctgggtgcga aaatggttgg taatttgcag cactccgata gaggtctctg agaccatatt
     8101 cgccaatatg gacaccgcga aaagctggat catcggcaag gcctgcatat ctttggcaat
     8161 atatttggcg cccagtggag cgagcaaaag caagacaaaa aacgccacaa ccgaagtgaa
     8221 cccttcggtc agcatcgcca ctttaacaac cgcgccggct ttttggcggt tattttcagt
     8281 caactcctta cccaggtagc ggatgatgaa ctcgcccatg cggaaagaaa aaacccggtt
     8341 cacattgctg acaaaagagg tgatgattcc caccaacccg aaggcagcca cccccagcag
     8401 gcgggcggca aaaacgcttt gcacaagcga aagcaacaaa ccaaatgaag ttgaactgaa
     8461 caggtacccg gtatttttta aaacccgggt gagaatgtgg tcttcaacaa ggagtttacg
     8521 gatcctggca aacagcgagt tcatgggagg gggatgtcac gagcccacgc gcgctgctca
     8581 aggtaccagt tcaccagctg agggatgcct tctgccagca gaaccttggg ttcccagccg
     8641 agcagactgc gcgccttggt cacatccgcc cagttggcat tcacatccgc cgggtgccct
     8701 gggatgtaat gcacgtttgc cttttttcct accaggtgct ctatccgctg taaaagttcg
     8761 ttgatggtga tggtttcgtg tccgccaagg ttcacaatct cgtaccccag cggttttaac
     8821 gccttaatca caccatcagc gatgtcgtcc agatacgtga aaccgcgcat ctggttgccg
     8881 tcaccagaaa cacacacagg ttcattctcc acgatccaac gcgtaaagcg atacatcacc
     8941 atatcaggcc ggcctgccgg gccgtaaacc gtgaaaaaac gcacaacggt cgtgtctaac
     9001 ccatacagat aatgataggt atacgccatc acttcggcag ccttcttact ggcagcgtag
     9061 atttgcagcg gatgacagga atccgcgttc tcaggcgtgg gcagcggagg gttggcgccg
     9121 taaatcgaag acgtggacgc cagcacgagt tttggcgtcc cgctgcggcg gcaccattca
     9181 agaaggttca gcgtcccggc ggcgttcgtt tgcaggaaca cctgggggat ttcaaccgcc
     9241 tgccgtacac ccgctcgagc cgctaggtgg atcaccccct ggaatgatgt aaattcgccc
     9301 gaaagcgatt gatagaactc cggtctgcag atatcatcgc ggaaaaagcg gaaacccggc
     9361 agccgggcaa gttgctgcaa acgccattcc ttcaggcgcg gatcataaga gtcgttaagg
     9421 ttatcgaccc ccaccacctc gtgtccctgg gcgataagct gctcgatgac cctgcgggcg
     9481 ataaacccgg cagcgcctgt aaccaggtag cgcgccatta gagttttatc accttgggag
     9541 atttcacgcc cagcttaccc aacgcgttac gggtatcgat aattaacttt gcggaattaa
     9601 ggatagccgc gtaatcgtaa accttatggt tcgtaatgat gaccacnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnccaa tgacaaaacg cggcatgttg gtgttaattt
     9781 cagaggccag ttcgataaag cgtgcggtgt aattgagaga gcgcattttc catgagaggt
     9841 acatcgggtc aatcgggata caatgccctc ccagaccagg cccggggtta aagcgcataa
     9901 acccaaatgg cttggtggcg gctgcgtcaa tcacctccca tacatccact cccaatcggt
     9961 cgcacatcag ggtcaattca ttgacaaagc caatgttgat catgcggaaa gtgttttcaa
    10021 gcagttttgc catttccgca acctcggtgg aagaaaccgg cacgacggta tccagcgcca
    10081 tgctgtacca ggccgtagaa acctcggtgc atttttcggt catgccgccg ataaccttgg
    10141 gggtattttt ggttgtccaa tcttcccgcc caggatcaac gcgttcaggc gaaaaagcca
    10201 ggaacagttc ctcgccgatc ttaagcccgc ttttctccgt cagcgcaggt aaaaccagct
    10261 cacgggtggt accagggtat gtgctggatt ccagcaccac caccatacca gggtgcacat
    10321 acggggcgat tccctccgcc gcgctgatga tgaaggacag gtcagggtcg cccgtgtggc
    10381 gtaacggggt ggggacgcaa atcgctaccg cgtcgatctc tttcaggatg gcgtaatccg
    10441 tggtgccgcg taacttgcca tcggccacca gctgcctgac caccagcgag ggtacatcca
    10501 ggatatagct ttctccgcgg ttcaacatct ccatcttttg ttcaaccggg tcgatccccg
    10561 tcacggcaaa ccctgcctgg gcaaaaaccg ttgagaaggg cagccctaca taccccatcc
    10621 ccagtacagc cactttggca gtctttttct taaacttttc aatcaactct tgcgcgtaat
    10681 ccatatacca ccttgagaat tcaaaatagc gataactgtt caggtttttc ttcgatctct
    10741 ttcacgcctt ctgccgaggt gcgcgcctgc gcgggttggc gtttcgctgc agctctggcg
    10801 ctggcaatcg cggctggtag tgtccgaaaa tcgttgatca gtgttgattt gagcgaaggc
    10861 tggtgcagcg cggctgccgg gtgatacatt ggtacaatga gacgaccacg cacccagtaa
    10921 gttttcgcgt gaacctcgct aatcctggcg ttcggtaaaa atttcgccat tgaaaacctg
    10981 cccagggtca caattacatg cggattaatc gtttcgatct ggcggtcgag aaagcgcgca
    11041 caggccgcca actcatcagg caaggggtca cgattgttag gaggtcggca ttttaccaca
    11101 ttggcgatga aaacctgctc ccgcgtgtag cccgcttctc ctaacagttc atcaaggaat
    11161 ttgcccgcct gacccacaaa cgggcgcccc tgttcattct catgaaaacc ggggccttcg
    11221 ccgataaaca ttatttcaga agtgggcgaa ccttcaccag ggacagcgtt cttacgtgaa
    11281 tggtacagcg tgcatgcctg gcaggtagcc acctgggcgg ctatttctcg aagtatctca
    11341 gcggtgtcca ctcacttcct ccacttggaa aatcaccaaa aagtataaac cttattttac
    11401 cttactggaa gcgcttcagg ttctccaggt tcaatggcgc aagatccatc agtatcgcgt
    11461 cttcctggtt gtctttgtag taattacggc gcacggctac ctcacggtaa ccgaaacgtt
    11521 gatacagcgt gatggctgcc tggttgctcc tgcgcacttc caaaaacgaa ctgttcgctc
    11581 ctgaatgata ggcatcgatt aatgtgtgcg ccagcaggcg ttgggcgatc ttctgacggc
    11641 gatacgcagg cgacaccgcc agcgtggcca catgcacttc atctgcgatc atccaaacca
    11701 cgataatggc gaccagcagc ggcgcgccgc tctcggagag cagttctgcc acccagcagc
    11761 gggaaacctc gttgttctca agttcaaacc ggaaagaccg ttcaggccag ggaagagaaa
    11821 acgaggagcg gtccagttca aaaacctgcg gcaggtcttc gctctgcatc cggcggatgc
    11881 gcaaagtgcc agacacttct gtttcgctca ctcctgaatc atccctgcca cgtgcagata
    11941 gatgggcgcc aacagcgcag ggtcgtccac ctctccttca ttccagcgtt tccaggcgag
    12001 ttctgccagc atggaagggc gccgcattgc cagcgcgggg ggcgcgatca tggcgttttt
    12061 ccacttgcgg ccaatcacct gacgctctga agggctcatt tcaccgcaaa tgcgggtcgg
    12121 tccgctgatg cggtcagata tttcttcagc ggtcatcacg cgcgcctcat cctggggctg
    12181 ccagcagccg ctgctggaag aaaactcctg cactgccagg cgcgaccttc ccgcgggcaa
    12241 cacagtgagc aggggttcag ctgccagggg ttgctgtgct gcttgaaatt caaaggttgg
    12301 gatgccaagc aggggaatat ttaaagacag agccaaccct ttggccagtg ccaaaccaat
    12361 gcgcaggctg gtgaaagacc ccggtcccag cgccaccgca acagccttaa gttcagcggg
    12421 gctcaccatg cagcggttca tcaggtcctg caccgctggc gccagctcta ctgtgtgatg
    12481 agaagccgtg cgccaaatca tttcgccgat aaggacaaat tcatcgtaca acgcgatgcc
    12541 gctggtttgt gttgaggtat caatcgcgag cagcatctac cctccaaacg ccaatcgctt
    12601 aaagtcgtcc agcatctttt tatatcgctc gccgacaggg ataaacaaca ggttgcgctg
    12661 ctcttcgttc aaccaggcgt atttgatcac cagcctatcg gctggcagca ctgactcaat
    12721 ccgctcagcc cattctgcca ccagaattcc gccgctgagc atatcatcca ggtcaagatc
    12781 aatggcttca tcaatagaag taagccggta agcatccagg tggtgcatca ggccgccgcc
    12841 agctcggcga tattgattaa caatcacgaa tgtcgggctg gtcactgcgt ccaccgagcc
    12901 ccagccatgg gcgagcccct gcacaaaagt ggttttaccc gatcccaggt cgccttccag
    12961 gcagagcaaa tcaccacttt taagaagggt gcccatgcgc atgcccaacc gacgggtttg
    13021 ctcggggctg cggctgacaa aatcaatact ataagcgggt aagataggca tcggtttaat
    13081 gatcagattg cgcgataggc tcactggtaa acatcggatg gcgggttttc ttgcggggca
    13141 tcaatcggga ggtaagtttt catacggcgg gtcaattctc ggcgcgtcag catcacgcga
    13201 tgagaacagg tatcacactc caacccaata tccgcgccta acctcacgat tttccaggta
    13261 taacccccgc atggatgggg tttccgcagc ctgaccctgt ctcctaattt caactcagga
    13321 agcatgctgc gattataacc catgttataa ttttcaaact caccacaact ggctgggctt
    13381 aagaggaaat tacccatatg ttgaacctgg caccaagcct gttattttcc cgcatactca
    13441 cattgatcat cgcgttaacc atccacgaat tttcccacgc gctggtggct gaccacttcg
    13501 gtgacgttac cgcgcgcaac gccggcaggc tgacgcttaa cccattaaag cacctggatg
    13561 tattcggttc tctgatgctg ctggtggtgg gttttggctg ggcaaaaccc gtgccggtca
    13621 acccttacgc gctgcgccgc aggtcagagt cggcgctgat gtgggtatcg ctggccggtc
    13681 ccgcgtccaa tctgcttctg gctatcctcg ccgggctggt gctgcgcttt cggatggtcc
    13741 cctggcgcgc atctgccgcg ggctccatgc tcccatcccc ggctgaattc ctcttcacct
    13801 tcctttccat caacctgctg ttgatgttgt tcaatctcat ccccctttca ccgctggatg
    13861 gtgaaaaggt gctctcctac ctgcttccct atcgtctaca gccagggttt gaaagcatcc
    13921 agcgctacgg cccgctcttg ttaatggtgc tgttatttgt tcttccgctg gtgggaatcg
    13981 accttatccg ttgggtgatg tcgcccgccc taacccagct tcaaaagttc attataggag
    14041 tcggttgatg agcccgcaaa tcgcactcat tggtctcaac caggttggcg cctcgatcgg
    14101 cctggcgctg ggcgcttaca aggaaaatat tacgcgggtg ggtcatgacg ctgacagcgc
    14161 ccggatgcgc aggctggaaa aagaaggcgt gtgcgataaa acccataccc gactgcccga
    14221 cgcggtacgt ggtgccgata tggtcatcct ctctctgccc actgacctgc tgcgcgatgc
    14281 gctggggctc atcgcgggcg aggtttttcc acaagctgtg gtggtgtgca tcagcgcggt
    14341 tgccagggtc gcttaccagt gggcgcgtga acttctgcct ccacagcagc cctttatcgt
    14401 tctgcaacct gtgttgcatc ctgccaggct gcgagactgg gaagagaatt cgagcaaccc
    14461 gtatgccgac ctttttgaaa acacacacat gctggtggta accgattcaa acacccagga
    14521 aaaagccttt caactggcaa atgacctgtg cgcgttactg aaagccaaac cgtattttag
    14581 tgaaccgctg gaagctgatg gcatcaccgc gcgcatcgag caactgccaa aactgaccgc
    14641 ggtagccctg ctttccatgc tggtggaaaa acccggatgg gatgacgccc gcaagatgac
    14701 caacagggct tttttccgct tcgcttctct caataccttg tttgacgaac aggagttctt
    14761 cggtgtttcc aatttggtga acagagaaaa catcacccgc gcgctcgatg aattgtccgc
    14821 gaccctggct gagattcgcc agatgatcga tgctggtgat gaagaggggt tgcaaaaaat
    14881 gtatcaaacc gccaggcagg ggtacgaaac ctggctcaca caacgcatga gcggggaatg
    14941 ggataaggtg gaaaaacccc ccgaagcgct ggaacgcggc atccttaccc gcctctttgg
    15001 cggcagacct gtcaagaaat aaaaattgga gatgagcttc tattgttaca ttgtcgaatg
    15061 cgctgacggc agttattaca ccggttggac ccttgaccct gcccgtcgtg aacggcagca
    15121 taacctgggg aaaggagcgg cgtacacgag agtacaccgt ccagttcggt tggtgtatat
    15181 cgaagaacag cctgaccgct caacagcgat gaagcgtgag cgcgccatca aggcgctaaa
    15241 tcacacgcaa aaggccaggc tgatcagcaa gtttactgag atgggcgatg accgtggcga
    15301 cccctgaagg taccgtcatc tcccctggca gagtgaacct gctgggtgag catgttgatt
    15361 ataacgacgg cgttgtgctg ccagccgcta ttgaccgttc ggtgaagatc atcttcaaga
    15421 agaatgacag ccgcctggta tcgctcaaag ccgttgacct gcaagaccaa tgcgtcatcc
    15481 acttgagcca tcttgaaaag aaaacgtcgc aagaaggaaa accattaccc gattgggcgt
    15541 tataccccgc cggcgtgatg tggagcctac aacgccacgg gcttgatcta atcggattcg
    15601 acgcccaatt cagttccaat attcccatcg gcgcaggcct gagcagctcc gcggccatcg
    15661 aagtggggtt cgcggtggtc tgtatgagcc tgtgcggctg gcagcttgaa cgcctggaac
    15721 tggcgaagct ctgccaacag gctgaacgcg agtatgtggg tgtaaattgc ggtttgatgg
    15781 accagttcgc ctgcgcttgc ggagtggaag atcacgtggt aatgttcgac acgcgcagcc
    15841 tggcttctca aacactgccg ctccctcccg ctactacgct ggttatcgcg gattcctcat
    15901 tacggcgttc acttctcacc tccgcatata acgaccgccg ccgcgactgc gaaagcgtgg
    15961 tcgcttattt taatcaactt gacccatcaa tccgctcact gcgcgacctg ggggaggaac
    16021 gcctgctcgc gcatgagcac gcgctcccgc cagccgcgtt caggcacgcc cggcatgtgg
    16081 tggaagaaat ctcgcgcgtc aatcaggcga ttgatctatt aaaggaaaaa gacgccaggg
    16141 gatttggtag attgatgttt tcctcgcatg attcgctgcg cgaccttttt gaagtttcgc
    16201 gccccgaact ggacattctg gttgatattg ccgccgggtt cgatggctgc ctggggtcgc
    16261 gtctaacggg ggcaggcttt ggcggcagca cagtcagcct ggtgcttgat gagaaaagtg
    16321 atgccttcat tcatcagtta agagacctct accaaaaaca gaccggattg caggcggtcg
    16381 ttttttccac ccgcgccagc cgcggtgctc acctcaatcc tgattagaaa ccaccggata
    16441 tacgccatgc tgacatctac aaatgatcgc gccattgaaa cccactcgct ggtgaagacc
    16501 tttcacacca aacatggctt cctcacacgc accacgcgct ctaccgaggc ggtgcgcggt
    16561 atcaccttcc atgtgaacaa aggagaactc ttcggtctgc tcggtccaaa cggcgccggc
    16621 aagaccacca cggtaaaaat gctctccact ctgctcattc ccaccagcgg cagcattcat
    16681 attttcggtc aggacattct gcagcacacc agggatatac ggaagcacat cggtttcacc
    16741 tttggcgggg ctcacgggct gtatgggcgg ctgaccgcaa tcgaaaattt gcgatatttc
    16801 gctgaactgt acgccttgcc cccccgtgaa acccataacc gcatcacgga gcttctggag
    16861 ctggtgggtt tgcgcgatcg cgggaatgac cgggtcgaaa catattccag cggcatgcag
    16921 cagcgcctgc acctggcgcg tgttttactg cacgaccctg agctgatctt cttagacgaa
    16981 cccactgtgg gcattgaccc ggttggagca cgtgaactgc gctcgactat caaactgtta
    17041 aaaaatcaag gcaagaccat tctgctcacc acacactaca tggcagaagc agatgagttg
    17101 tgtgatcgtc tcgccatcat caatcacggt caaattatcg ctatggatag tccttcttca
    17161 ttgaagagca aattgaccag cgatgccttt gttgagttaa aaaccagcgg cgaaaacctg
    17221 ccatccgtaa aaaacgtgct cgcccagctc gacccggatt cagaggtcac ctataaccag
    17281 cacgctttcg tctgggaagt gaatatccgc actcggcaac caggtgtggt gatggaactg
    17341 ctcgctcccc tgctcagcag caacctgata cagacgctgg gggtccgcaa ccagaccatg
    17401 gaggatgttt acgtgtccat cattcaaaat ggaggggcat gatagggaat tttctcaggt
    17461 tgggaattcg ccagatgaat gtgcaactgc gcatgcgtgt gctcagcctg tggagcctgg
    17521 ctttattttt cacacagccg gccattttca gcgccatcgg catgctgctg gcgcgcgccg
    17581 ttggaaacac ccgtcctgac ctggtgtaca ccgtgcttgg cgccggcatc atgggtatgt
    17641 ggagcggctt ggtgttcact tccacctacg atatccgcgc tgacaggcgc gagggcactt
    17701 tggagttgat cgtgggcagc cccactccgc tgcggcgcgt ggtgggattt cgctcgctgc
    17761 tcaacatcct ggcggggtca accagcatgc tcgccgcggt tgccgcggct ttatttatct
    17821 tcgattattc attcagcggt atcaatatcc ctggtgtcgt catctccttc tccctgcttt
    17881 tactggcact gtggtgcatg ggcatcttcc tggcgaatat actcgcctgg tcgcgccttt
    17941 ccggcacttt cgtcgattta ctggagatgc ccatcgcgct tttgtgcggg tttatgtacc
    18001 cgatcagcgt tttgcctgct tggatgcagc agatttccgc tgtcttcccc atccgctggg
    18061 ggctggagac tttgcaggat gcgctaaaag gctcgcttat cgacagcgcc ttggtaaaca
    18121 aatggctgat cgcgctgggg gtttcattcg tctactatct gctggcgcgc tggtttgata
    18181 ggaaagtgca cgatcatatc cgcatcaccg gggagctgca ttcgatatga tcaagcggct
    18241 tgggcacctc ttccggctgt actttttcca ggcatggttt tcataccgct ccctttttgc
    18301 ctggagcacg ccattcagtt acgcgaccag caaattcggc tttcccttct tcgccatgtt
    18361 gttcttcgtg ttcatgggta agtttgtcgg ctttctggat ccaatgttca tcgttattgg
    18421 caatatcttg atgatgccta ccactaacgg gttatacggt atttcgatga ccatcgggaa
    18481 cgagcggcag tttggcgcca tgtcttacct gttgggcagc ccggcgccgc gcgctccgct
    18541 ctttttgggg cgtgcctttt ttcacatcct cgacgggttt gtgaccgtgc tggtagccct
    18601 gcccatcgca atgctcatct tccatatcga cttcgccgag gtcaacctgt tcatgctcgc
    18661 ctgctgcctg gtgctgctat cgataaccac caccgggctg ggattcatca tgggcacggt
    18721 cagcctgctc agccgcgacg gctggatgtt taccaacacc ctcggtcagt tgctgtacat
    18781 tttgattggg gtcaacattc ccattgaaag actgcctggt tttttacagc cgctttcggg
    18841 atacctgccc atgacgcgcg gaattttagc cacccggctg gctctgggtg gagcgggttg
    18901 gtcagaagta gccagcctgt tggcggggga gattctggtg gctgctgtgt atatggtatt
    18961 cggctacacg cttttccggc tggtggaaag agccagtctg tcgactggct ctctggactc
    19021 catttaatga ggatgcgcag gcaaacagcc tgtgttcaat ccgtaaacac cggcaggtga
    19081 cccagggaaa taatttcgtg ccacctggcc cgctcccctt ctgtgagaat ggcggcttcc
    19141 gccaccacct cagcgccggc tttctgcacc agcaggcgca tcccctgcaa agttgaaccg
    19201 gtgctgatca catcatcaac gattacgacc ttcttcccca tcatcagctc gcggtctttt
    19261 tcatccaggt acaggtattg cggctcaccg gtagtgatcg aaagggtttc agccttaagc
    19321 gcatctccca tatagggttt ataaaccttg cgcagcacca cgtaaggttt catcgtctcc
    19381 gccgccagcg cgtgggcgat ggggatgctt ttcgcttcgg ctgtgagcag caaatcataa
    19441 tccacagcgg tcagtttttc agccagggcc gccgcgcagg attgtacaag ttccatatcg
    19501 cccaggatat tcagaatcgc gatttttagc ccgggtttga tctcaaacaa ggtcaggtca
    19561 cggtgcacgc cggcaatttc gatggggtag gtttctctct tcttcatatg acatctccaa
    19621 cagcctgata tgggaactga tgcgagcaaa agtttatcac aaatttttat gtaaaaacaa
    19681 gggattcccg cgcttcggtt tacgggaatc cttataaagg ggtgacctat ggtagaatca
    19741 tcgagtgccg gggacgccga ccccggcact ctttactaag gaggagaaga aaagaagtcg
    19801 ccctgtaaat agtgtaccag tgaccctctc gcactgcttg actcttaccc tacgattacc
    19861 ctacgaaaat gatggtcgca aaaaagcgcc attttcgctg taagaaacca tatttttcaa
    19921 cgaatgatat aaacttataa acatgcctga tagaattcaa tttcaaagcc ctattggaat
    19981 ggtcactgtt ctggtttgca gagatgctgt ctcagccgtt caaatcagca taaacaccgg
    20041 gaaacctcac cctgcaatga cgacaaaccc ggttttatgc gcatgccgac aacaaatggg
    20101 agaatatttc gctggtcagc gtaaggcttt cgaccttcct ctggattggc agtccatccg
    20161 ggggttccag cgtgaggctc ttctggagtt attgaagacc ccgtatggtg aagtgcgcac
    20221 ttacggtgag atttccaggg cattggggaa gccagccgct gcccgcgcag tggggatggc
    20281 actggcgaaa aacccgttac cgattctcat cccttgccac cgcgtggttg ctgcttcagg
    20341 gcatcttacc ggtttctctg ccggtgatgg cctgaactcg aaagcgtggt tgctacaact
    20401 ggaaggacat cgcgttgtca ctgaaaagct ggcttaaatt tattttgctt ggcatcgcct
    20461 ggggctcctc ttttttctgg attaagatag ggctgcagga agttggtcct tttactgtgg
    20521 tatttttccg cattttcttc gcgctaatcg ggctcaccgt ttttttcttg ctcaaccgtc
    20581 gtaaacccct gctaaaagcc tggcggttgt atctgttcct gggggtgttc tatgtagccc
    20641 tgccctttgc gctggttgcg tggagtgaaa aatacatcac ctcgggtctg gcctccatcc
    20701 tgaactcgat gcagccgctt gccacctccc tcattgccgc ggtgttcatt caagaagaaa
    20761 ggctcaacgc gcggcgcatc gttggtttgt tacttggctt tggcggcgta ctggtactga
    20821 tgtcagataa actcagcgga gaagccatca atcaaatata cggtatcatc gccatggtgg
    20881 cagccgtttt ctgctacggg ggcagcagcg tatttgcccg catgcacaat cagggggtga
    20941 aacctgaaga tcaggcgata gggcaattcg tctttggtat cctcttcatc ttcccaacca
    21001 tgttagcggt tgaatctccg ttcattatgc cagtgcgacc agtctcgtat ctggcattcg
    21061 cctggctggg cttgatagga tctttcttcg ccgccatcgc ctggtactca ctgctggacg
    21121 agattggtcc ctcgcgggtg agcatgacca cttacctgtt ccccctggtg ggtgttctgc
    21181 tgggggcgat cctcctgcac gaagcggtag actggcgggt gatcgtaggg ggtctgatga
    21241 tcctgggcgg gatcgttatc gtcaacagcg gaaaaagaaa caatcaaacc ccagcccagg
    21301 ttcaggccga cccatttgaa ggataaaatg atggaaaaac aagaattccg cgctgggtat
    21361 gtagccgtgg tcggcaggcc gaatgtcggt aagtccacgc tcatcaacgc gctgctcggt
    21421 cagaagatcg ccgccgtctc gccaaaaccg cagaccaccc gccgcaacca gctgggcatc
    21481 ctcagtctgc ctgaagcgca gcttatcttc gtggatactc ccggtttgca tgatccccag
    21541 cacaagctcg gcgatttcat gaaccaggct gcgggcgagg cgctgcgaga cgcggatatc
    21601 ctcctctggc tgttagacgc cagcgcgcca ttcaccagtg aagaccatct catcgcggaa
    21661 aaactgggcg gtatgcgcag ccttcctccg gtatggatgg ggttaaccaa gaccgacctg
    21721 gtgaacgaaa aaacccttga agaacgcaaa cgccaggcgg tagaactgta tccagcgcag
    21781 gacgtattga ctttgagcgt taaaaataaa gccagccttg acgccctgct cgaagtgatg
    21841 atcagccgcc ttcccctggg tgaagccttt tatgaaagcg atactgtgac ggatctctat
    21901 gaacgcgata tcgccgcaga gctgatccgt gagtcagtgt tgactttcgt gcaggaggaa
    21961 gtgccgcact gcgtggcggt gaagatagat gcttatgaag aacgaccggg agaaaaggct
    22021 tacatcgaag ccacgctttt tgttgaacgg gattcgcaaa aaggcatcct catcgggaaa
    22081 ggcggggaaa tgattaaaaa gatcggtact cacagccgcc aggagatcga agaaatgagc
    22141 ggttgtaagg tctttttaga attaaaggtg agagtgaaca agaactggcg gaattcgcca
    22201 gacgcgcttg aattattagg gtataaacag gttgcaggtt aaaacatgtt aacctgggcg
    22261 ttctggctcg cgggtttctt cgccctgctc gactgggccg ctgtctggaa aggttggagg
    22321 aaacgccttt acctggccaa gcccgcggtg atggtttttc ttatcttttg gacgctgcag
    22381 ctgaccggat ggcgaaatga gatgacctgg ttcggcgcgg gtttggtttt ctccttgatt
    22441 ggagatatag cgttgatgct taacccccgt ttcttcatgg tcgggggaac ttcgtttctg
    22501 ttcgcccagg tggcgtattt tattggcttc aatacttctc ccgcgccgct ctccggcggc
    22561 gtgttcctgg tggctgtgct ggtaggcgcg gctgctgctg tgatcttcaa acgcatccgc
    22621 ccgggcatcc tgcgggttcc gcgcggcagg cgttttcttg ctgctctctg cctttacggc
    22681 ttaacgctca cgctgatgct gctttcagcg ctgctgacgc ttttccgacc agaatggcaa
    22741 taccagccag ccatcctggc tgccattggc gcgctgctct ttttttcttc agataccatg
    22801 ctggtttacg accgttttgt acgccggttc aatcacgctc agagctatgt gcacctcacc
    22861 tatcacctgg cgcaattcgg catcatcagc ggtgcagtgc tgcactgctt gcgctaggtg
    22921 gtctacttca aaggtctgaa cccctcccct aacgcctcgt gcgtgacccc gatgaccata
    22981 aacgcctgcg ggtcgatctc tttgaccacc tgttttaatt gattgacttc agcgcgggtg
    23041 atgacgcagt acaaaacagg tttatctgtg ccggtataag cgcccttgcc gctgagcacc
    23101 gtcactcctc gtgagagcac agacatgatc tcgctggaaa tcttttcatg gtatggtgag
    23161 atgatgatgg cagtgcggaa tatcccggac ccctctgaga tcatttccgc tgccagcccg
    23221 ctgatataaa tgaccaccat gccataaagc gctttttccc aagagaacgc gaacccgccc
    23281 gcgagtacaa cgatggaatc ggtaatgaga tacgccgtcg agatcgatat ccccagtctg
    23341 tggttgagga tgcgccccag gatatcagag ccgccactgg tgccccctcc gcggtagacc
    23401 agccccagcc ccacacccag caacaccgct ccgaaaaccg cgttgagcac gttgtctcgc
    23461 gttacgcctt gcgcggggat gataaatacc agcaggtctg tgaacaaaga gaaagcgcct
    23521 accgcgatgg cggtgcgcaa cgcaaagcgc ggaccgccca aaaaccgcca tcctaaaaag
    23581 aagagcggga cattgccgat gaacaccatc aacccaatgg gaataggcgc gaaatgattg
    23641 atgatctggg cggccccgct gattccgccg ctgaccaatt gacctggcac caaaaaaagc
    23701 cgcatcgcca gcgcctggat cagcgcgccc agcagcacta ccaggtactc gcgcgcggtc
    23761 ttccacgtga attcgagcgg cccgatcgca gaagtgaagc gaggccataa ggcagttaca
    23821 gttccttttt gagcagtcat tagaaacctc ttctatgaag ccgcacaatt gtaacagata
    23881 gacgatgaaa aaatgggggt ttcgcttgac ggtagttgga ggtagtggta ttattgggtt
    23941 tcttgatgcg gggtagagca gtggcagctc gtcgggctca taacccggag gtcagtggtt
//
LOCUS       DAOSPM010000022        23869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2137_length_23869_cov_12.900983, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000022 DAOSPM010000000
VERSION     DAOSPM010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 23869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23869
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2137_length_23869_cov_12.900983"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..2149)
                     /gene="fusA"
                     /locus_tag="PLW25_03430"
     CDS             complement(80..2149)
                     /gene="fusA"
                     /locus_tag="PLW25_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOS53572.1"
                     /translation="MSRNFPLDKCRNIGIIAHIDAGKTTTTERILFYSGRTYRLGNVD
                     EGTTVTDWMVQERERGITIVSAAVTAEWKGYQINVIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDAVQGVEPQSETVWRQADRYSVPRICFVNKMDRVGASLEYTLGTIRERLGAQPI
                     QMQLPIGSEAGFKGVVDLLTMQAVYFEDDLGKEPKYAPIPEDLRKQAEDLREQMVEQI
                     VELNDGLTVKYLEGEKISIDELKQALRAAVINTSATPVFCGSSLRNKGVQLVLDAIVD
                     YLPSPVDIPPVIAHSPDGEKEMELPTEDNAPLSALVFKIVSDPYVGRLAYFRVYSGTL
                     KTGDTVLNPIKGRRERIGRLLRMYADRREDITEVTAGDIGAVLGLKESFTGDTLCSVN
                     KPLILESITFPEPVISIAVEPKSTADQDKMALALQRLAEEDPTFKVHTDESSGQTIIG
                     GMGELHLDVLVDRMLREFRVQANVGKPRVSYRETITREVKEIDYKYVKQTGGHGQYGH
                     VVLNLAPLDRGAGVKFESKITGGVIPREYIPAVEKGVKEAVESGVLAGYPVTDLQVQL
                     VDGSFHEVDSSEMAFKMAAILGMREGLQKGASVLLEPIMRTEIVVPEEYLGDVLGQVT
                     SRRGVVLGMDVRPGNAQAIHALLPLSDMFGYATELRSATQGRGVFTMEFDHYAPVSAA
                     VAEKIIN"
     gene            complement(2173..2640)
                     /gene="rpsG"
                     /locus_tag="PLW25_03435"
     CDS             complement(2173..2640)
                     /gene="rpsG"
                     /locus_tag="PLW25_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOS53573.1"
                     /translation="MRRSKPEPREVTPDVRYNSVPVTYFIHRIMKRGKKSVATTIMYD
                     ALEMIKEKTGKSPVEVLDLALKNVGPVMEVRPRRVGGATYQVPMEVPAHRRMTLAMRW
                     ILDAATSRTGNSYAEKLSTELIEASNNQGNAVRKREETHKMAEANRAFSHYRM"
     gene            complement(2666..3085)
                     /gene="rpsL"
                     /locus_tag="PLW25_03440"
     CDS             complement(2666..3085)
                     /gene="rpsL"
                     /locus_tag="PLW25_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOS53574.1"
                     /translation="MPTINQLIRKGRETKRAKSKAPALQYTLNSMKQRRSRLEKGAPQ
                     KRGVCTVVRTMTPKKPNSALRKIARVRLSNGIEVTAYIPGEGHQLQEHSVVLVRGGRV
                     KDLPGVRYHIVRGTLDTSGVAKRSQARSKYGSKKPKA"
     gene            complement(3290..7207)
                     /locus_tag="PLW25_03445"
     CDS             complement(3290..7207)
                     /locus_tag="PLW25_03445"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOS53575.1"
                     /translation="MASVLPTKNYARIPTIFDLPNLIEVQLESFIRLKEEGLEDLFHE
                     ISPIESYNKGMKLYFPGRSPEAKQWGLKYWFEPPKHSIEECVERDLTYASPLYVSVLL
                     AGPDIPEPVKSDIFLGDFPEMTEKGTFIINGTERVVVSQLIRSPGVYFEAPVDRATGH
                     RLAMAKLIPDRGAWMEFETRKNDYLILKFNRKRTVPITIFLRALAAVDDLLPGESPVK
                     TGSDEELIALFSDIDNNPDRQFIPSTIRQEPEWDLTHYQTIAEAALIEFFKRMRPGDP
                     ANLENARQFLQEQLFDSRHYDLERVGRYKLNQKLDLMSRIPVSHRSVTKWDIVYLVRR
                     MILINNEIEDKDDIDHLGNRRVKTNGELIQNKLRIGLRRMERVIKERMSIRDQDQVSP
                     VSLINIRPVVAALREFFGSSQLSQFMDQTNPLAELRSKRTLSALGPGGLRRERAGFDV
                     RDVHHSHYGRICPIETPEGPNIGLIGRLASYARVNEYGFIETPYRKVLKTLAYDDPRL
                     AGRLLRESLVDEKSGEVFAPEGERVTAKMLSAIKKTKKEEIHVAPFVTEEVEYLSADA
                     EDKYVIAQANTILNENNEFAQNQISCRHHSDFILSTPESVQYMDVSPHQVVGISAALI
                     PFLEHDDANRALMGSNMQAQAVPLVNPDSPMISTGMERYAALDSGQVVVAHEDGDVIS
                     VTGQQIVVKNAEGKNDVYHLRKYQRSNQSTCIDQRPAVVKGQRIRKGDILADSSSTVG
                     GKLALGQNVVVAFLSWEGGNFEDAILISERLVQEDKFTSVHIEKYEVEARDTKLGPEE
                     ITRDIPNVGEDAVKDLDEQGIVRIGAEVGPNDILVGKITPKGEKELTPEERLLRAIFG
                     EKSRDVKDTSLRMPHGERGKVVDVKVFTREDNVDLSAGVDMMVRVSVAQRRKITAGDK
                     MAGRHGNKGVVSRVVPVENMPFLKDGTPVDIILNPLGVPGRMNIGQVLETHLGWAAKE
                     LGFEAITPVFDGANEYEIEAELARAWLMDYTWTEAGKRAWEWLNGIDHDPESIKDDDE
                     VRLLYLEQTLDHKYDMGRIATDPTYARQITLESWLNERGFDLAPILDSADPNTEAERK
                     ERDTQAINTCLRVWLEVNGTDPSKIKDEKIREVADKVALETRQPVPTLGKQILRDGKT
                     GQAYDQPVTVGVMTMLKLHHLVEDKVHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEV
                     WALEAYGAAYTLQEMLTVKSDDVQGRVNTYESIVKGEPIEDPSLPASFRVLVKELQSL
                     GLAVEAVMDTKEIIRFGKDEERVRPPRLETGLLGLGEELQGLL"
     gene            complement(7426..8022)
                     /locus_tag="PLW25_03450"
     CDS             complement(7426..8022)
                     /locus_tag="PLW25_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOS53576.1"
                     /translation="MSDPEEIRIQSEKRIVAAVLADTHVIDRVGRLHPHLIPLIKNSM
                     ADLIIHAGDISHPRVLEQLGELAPVYSVRGNRDLYFLRSLPVMQRLNLNGARVVVTHG
                     HLGVAGYWRDKLENTLHGYQFARYYRRLHSLAVNADVVIFGHSHHAENQLREHVLFFN
                     PGSCSVAEKPDYRLSFGVISVSPQAEVKAEIVWLEGHP"
     gene            complement(8015..8623)
                     /locus_tag="PLW25_03455"
     CDS             complement(8015..8623)
                     /locus_tag="PLW25_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOS53577.1"
                     /translation="MPGTIKAVIWDMGGVLLQLVDDRPRQELAKKFSVTLGELDKLVF
                     NSETARLATLGAITEEEHWQQVGKTLGVPPADLRDFQLQFWTGTRVDDRLIEFIQKLR
                     GSYQTALLSNAWSGARKALTTYFDCLHVFDHIVISAEVKMAKPDPAIYLDVLKRLGAE
                     PYQAVFVDDLQENIDTANWLGMHGIVFENSEQAMADVESKLV"
     gene            complement(9161..9745)
                     /locus_tag="PLW25_03460"
     CDS             complement(9161..9745)
                     /locus_tag="PLW25_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOS53578.1"
                     /translation="MIGTQKALVPGIIFSCLLLFLLQQLVKPIGAASAAAEPLRTMET
                     DQATGAEISPQACQFHDRYPAGVVEWCGLIEANAQTYGIDPLLIAAVMAIESGGQPEV
                     ISVSGAVGLMQVMPSDGIAAGFQCINGPCFSSRPTIAELKDPDFNVMYGTRMLAGLIE
                     RAGNARDGLKSYGPADVDYWYADKVIQLYESLNW"
     gene            complement(9836..10906)
                     /gene="gmd"
                     /locus_tag="PLW25_03465"
     CDS             complement(9836..10906)
                     /gene="gmd"
                     /locus_tag="PLW25_03465"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOS53579.1"
                     /translation="MKKALITGITGQDGSYLAELLLSKGYEVHGIIRRASTFNTRRID
                     HIYHDPHRNGEEVKLYLHYGDIAAIESLMDVIYNVRPDEVYNLAAQSHVRVSFDMPEY
                     TGDVTGLGTIRLLEAIRRSGNPERFYQASSSEMFGAAKPPQNELTPFEPRSPYAAAKV
                     YSYWVTRNYREGYGIFAANGILFNHESPRRGETFVTRKITRALAAIKAGTQSKLYLGN
                     LDSKRDWGYAPEYVEAMWLMLQQDTPDDYVIGTGEAHSPREFLEEAFGYVGMDWKKYV
                     EIDPRYFRPTEVDYLLSDPAKARARLGWEPKIKFSELVRIMVDADLELSGQKSPGEGK
                     KILTERFNGWHRWDHQVVSMER"
     gene            complement(11086..13527)
                     /locus_tag="PLW25_03470"
     CDS             complement(11086..13527)
                     /locus_tag="PLW25_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS53580.1"
                     /translation="MMRFDRFTERAQEAAQRAAEITQRYGHNQIDTEHILLALIEQPQ
                     GVVPQILALIKVDPQILEERLDYILKTSPKASIYGQGAGQVFITPRVKRIMDLANEEA
                     SKLKDEYISTEHLFLAILSERNTPAAKLLETTGLTRDRVMEAIVELRGGQRVTDPQTE
                     SRYRTLDKYSRDLTQQAKEGKLDPVIGRDNEILRVMQILSRRTKNNPVLIGEAGVGKT
                     AIVEGLAQKIASNDVPEILMGKRVISLDLGAMIAGSRFRGEFEERLKASIEEVQRSSG
                     EIILFIDELHTVVGAGAAQGAMDASNMLKPALSRGELQCVGATTLDEYQKYIEKDSAL
                     ERRFAPVFVDEPSPEDTLKMLMGLRDRYEAHHKVHFSDEALSAAVKLSSRYVSDRRLP
                     DKAIDLMDEAAAKLRVALYSMPPELKQAKSEIERLKAEEELAGVERDYERAARMKAER
                     LRLEEQFNQDRDKWESDHKLDEVVDVDDIAQVLAQWTGIPVSQMLEEESERLLKMEDR
                     LHERIIGQDEAIRAVSDAIRRARSGLKDPRRPIGSFIFIGPSGVGKTELAKALAEFMF
                     DDEDALVRLDMSEYREQHTVSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVILFDEIEK
                     AHPEVWNSLLQILDDGRLTDGQGRVVDFRNTVLIMTSNLGTEFVRQGGALGFLQKSDT
                     DEDRQAQEKIEKALKSTFRPEFLNRIDEIIMFSALTPEQMLEIVDLQMNEVRSRLEER
                     GLKVELTDEARTWLAKEGYDPSFGARPLKRALQKYVENPLSVTLLSGEYGDGDTVLVD
                     LDKEKQEITFRKQEK"
     gene            13717..14703
                     /locus_tag="PLW25_03475"
     CDS             13717..14703
                     /locus_tag="PLW25_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS53581.1"
                     /translation="MDERKTDPRIRPIEIQRDLPAIADLIETAFAEQMDDEGRDYLRH
                     LRHIAAGAGAFILDGSTPESSTLPFHGYLWEENGRVIGNLTLIPLRKRDHKSYIIANV
                     SVYPEWRGKGIARQLTDRAIAHVREHDGRHIYLQVRADNPPAIHIYQHAGFEEFTRRT
                     SWVFSKNNLHPPILPPGVDVRRRKRADWAQQKDWLQLTYPPEITWNLPYSLPRLRPDF
                     WSWVENLLSARVCRTWSAQKAGRLIGSASYENGYTGSDYLWLASSPAWEDIAIQALLP
                     HVYTRVLKPQRIVVNYPAGRGINGFTACGMKELHTLIWMKMEIPSRLYSLDG"
     gene            14750..15124
                     /locus_tag="PLW25_03480"
     CDS             14750..15124
                     /locus_tag="PLW25_03480"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOS53582.1"
                     /translation="MKRLLLKVLINAGALFLAVWLLRPHIVMQNDAWYAYIVLGAIFG
                     LVNALIKPVVMFVSCPLVVLSLGLATLLVNTLMFLFAGWFGTVLNIGFTIPQQQFYYA
                     FLGALIVSVVSFILSRILDSSQ"
     gene            complement(15189..18902)
                     /locus_tag="PLW25_03485"
     CDS             complement(15189..18902)
                     /locus_tag="PLW25_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HOS53583.1"
                     /translation="MNSAENVESKPGWLDRPLYTWIPRITIEHIVIALIILMAVLSRF
                     YAVGLRVMSHDEVNHVVPSYELYQGRGYAHDPVTHGPLQFHLVAGSYFLFGDSDFSSR
                     IPAALFSIATIIFVIFGFRRYLGRVGALLAGAFFLVSPYMLFYGRYTRNEAFVALFGV
                     VMLYAVLRYLEDGRHRHLYLFTVALALHFTAKETAFIYTAQILIFLAVLFIRDVVKHE
                     WPQGGRRDFFIFSMMAGLLFVGTALGAAVMDASIKPGNAEAVAAPAVYHTVMLISLAL
                     AALGMILAIVVLLGSMGWRVVREIRSFDLLILTSTMILPQLIAFPINLLGWNPLDYSQ
                     TGMIRTLLFLLGAVVISVTLGLLWKPRLWLINVGVFYAIFTIFYTTFFTNGQGFFTGM
                     VGSLGYWLSQQGVNRGSQPWYFFAFLQVPMYEYLPLVGTLIAVVIGVKHRLFSSLPGV
                     APALQTAEQVEVPSQLPLPALEGSEETPAEKPGKLPVLSLLVFWGLTSLVAFTLAGEK
                     MPWLTVHVTLPLLLCAGFGFGYLVDSTPFKQLANKKGLLVLALLPVFITSLGGTIAAL
                     VGTQKPFAGKELEQLKATSTFLFALIATLASGGLLWKLLLRWSQRAILKLLTLTLSAI
                     LMVLTARSAYQASFINYDTGKEFLVYAHAARGPKDVLEQVEDISQRTTGGKDIRVAYI
                     GDALYPYWWYFRDYPNKVWLKDDLTRDLLNYPVVISDDEHYSKTQAILKDQYFETKYK
                     RLVWPMQDYFNMTWERFWKGFTNPQMRQAIINIWLNKDYRLYASLANNSGLTLENWQP
                     SANIYLFIQKDIVERIWTYGAIPQQQETAETDPYAGKYFELTPDRVFGSSGSAEGQLT
                     MPRGLAVAADGSVYVADANNHRIQKFSADGAFLLSWGSYATAEGGNAPGGTFNEPWGV
                     AVAPDGSVYVADTWNHRIQKFTRDGKFLTMWGVPGLAEEPDRFWGPRGIAVDREGTVY
                     VTDTGNNRVVIFDENGNYQQQFGRSGINPGEFDEPVGIAVDGNGRVYVADTWNQRIQV
                     FEPAGKEGYSVLRSWDVHGWFGQSINNKPFLALDAENHIFVTDPDGFRVLEFDQMGNF
                     LRGWGEVSSGIDGFGVPSGIAVAGDERVWVSDAENNFTLGFLLPPLAIPVEEGDVEFP
                     SESSLPQIPRGLSYQAESGLVVNYVEIPVYRLSDDGTEWVPLIPESISAQLAVEVLPE
                     KDANGDWLLRKDGKTQYKWDATGFKWVPADPVP"
     gene            complement(19021..20538)
                     /locus_tag="PLW25_03490"
     CDS             complement(19021..20538)
                     /locus_tag="PLW25_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOS53584.1"
                     /translation="MACTETDLMMKRVGHFSIFRWLSLAFILAALVLTVFQLVVFSRL
                     RSSFSTGTSIAGVDVTGLSMEQAASRVTQAYSIPVELRYNNSVIQVKPGTLGFSLDMT
                     AMMTAADQLRSAEPFWPSFIAYLFNQQPSASEIPLRAEIDDKQLRAYLVNEIASRYDE
                     EPAAYLPVPGSVTFEAGTPGKKLDVDRSALLVSNALRSPAARIVNLALITGKLNRPSM
                     DNLRILLQQIVDVNNFDGVVELYLQDLKNGSEMQMAYQNGESLTPGIAFSADSTIKIA
                     VMVTAYQEVAEPASTKVVQLLQSMIAMSDNTSTDNLMREVLSPTLGPLTVTSTMRKLG
                     LQSTFLDGMFYIGAPLLTGNIVTPANSRRDVFTDPDPYNQTTPTEMGLLLADIYQCAQ
                     NDGGSLVAVFPGEITPAECRSMISLLTQNRIGVLIEAGLPDGTQIGHKHGWAIDAMDG
                     VMHTVGDAGLVYTPGGNYVLVIFIYDQNQIVWDNANQLFADLSRAVYNYYNLTAE"
     gene            20537..22261
                     /locus_tag="PLW25_03495"
     CDS             20537..22261
                     /locus_tag="PLW25_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOS53585.1"
                     /translation="MEILPDFGTPLFAEKPRFFLSFSYTYSMETVEPATVSLLAGLFP
                     LTLLEGNDLSQVAAQFEVLNYPRGSRLYAADDIPDYFYWIISGNVRLAGGAMRRGKAE
                     QVLGAGDYFGAEVLSRSDYRFSDAVCLSEVKLVRVNEQDLIQLCANLPTIENAFSLIY
                     QTHKFRARLFFPWLEKGEKVNLVCRRHPFFLALRVVLIGGAVIASFAGLLSLALSSKS
                     VALILLAGTLLLIGTLLTAWSALEWGNDFFILTSDRVLVQKKLTGFFDNRQEAPYTAI
                     LSTGFETSFWGRLLRFGTINLRTYNGKLSFKRLPVPEIIFDLLEYQRRSIAQKKHQSD
                     YAEMRETLQHRLEGATVRTKNAAAGKHTGGLKTTYKSGSFLDLAARFFGLRHESAGAI
                     IYRTHWWRLLGKTFLPTLLLLIVVLLVLLKLAGFLPSIAADHLYIGSIILAVTSWAWW
                     LYQYQDWYNDVYIITSEQLVDVNRKPLGKEERRSAPVQNIQTVEYKRKGIIGLILNFG
                     TVHIQIGNQELSFDDVYNPAAVQAEIFSRFKHRDQKTLRLEQEKLVDWIKAYDEMKKE
                     DKDPFDSQ"
     gene            22305..22811
                     /gene="def"
                     /locus_tag="PLW25_03500"
     CDS             22305..22811
                     /gene="def"
                     /locus_tag="PLW25_03500"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOS53586.1"
                     /translation="MALREIITIPHLILRKKARKVTDFGRDFQVLVTDMIDTMREAPG
                     VGLAAPQVAESQRVIVVEYGDEEDEEKPKKLFVVANPEIISSSEDKVIGIEGCLSVPE
                     LVGEVERYHAIIVKGQNRFGKPTKIKASGWLARIFQHEIDHLDGVLYPDRALRVWKPA
                     PEEDIPLD"
     gene            22815..>23869
                     /gene="recF"
                     /locus_tag="PLW25_03505"
     CDS             22815..>23869
                     /gene="recF"
                     /locus_tag="PLW25_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOS53587.1"
                     /translation="MHLTHISLTNFRLFSRLDMNVPRRILLLLGANAQGKTSLLEAIY
                     YLATFTSFHAQNDRQLLNFIATRENLAVARLVAQYTRAERAHRLEVRLIQDSNGNGGA
                     RLRKEVLLDGVKTPPAQVIGQFNAVIFLPQMTGIIEDGPEERRRYLNLTISQSNPVYT
                     RALADYNQALTQRNALLKLLNERGGDRSQLDYWDEILAARAALLITTRAVVIQELEEI
                     AKRVHERLTGSKEVLRLNYQPSYDPLPAPQGQFALPMQVQVQRSGLSQEKVKQGFIAR
                     LAELRAEEIARGITTIGPHRDEMRVLSNGIDLTDFGSRGQIRTALLSLKLAEVDWLKE
                     KSGQWPVLLLDETLAEL"
BASE COUNT         5550 a         6624 c         5870 g         5825 t
ORIGIN      
        1 attcaaatgc ggcttactcc tgtcaaactt ctgcttcgcc atgtttcctc tccttagaaa
       61 ctaagttcca atgattgcat taattgatga ttttttccgc tacagcagcc gacaccggcg
      121 cgtagtggtc aaattccatc gtgaacacgc cacgcccctg cgtggcagaa cgcagctcag
      181 ttgcgtaccc gaacatatca gacaaaggta ataatgcatg aatcgcctgg gcattgcccg
      241 ggcgcacgtc catccccagc acaaccccgc ggcgcgaagt cacctgaccg agcacatcgc
      301 caagatattc ttcaggcaca acgatctcag ttcgcatgat tggttcaagc aatactgacg
      361 cgcctttttg caggccttct cgcatgccca atatcgccgc catcttaaac gccatttcgc
      421 tcgagtcaac ttcatggaaa gaaccatcca ccagctgtac ttgcaggtct gtcacgggat
      481 accccgccaa aacgcctgac tccacagcct ctttgacgcc tttttcaact gcgggaatat
      541 attcacgcgg aataacgcca ccggtaattt tgctttcaaa tttcacacca gccccgcgat
      601 ccaggggcgc caaattcaat accacgtgcc catattgacc atgaccaccg gtttgcttta
      661 catatttgta atcaatttcc ttgacctctc gcgtgatggt ctctcggtaa gaaaccctcg
      721 gtttacctac gtttgcctgc acgcggaatt cgcgcagcat acggtccacc agcacatcca
      781 ggtgcagttc gcccatgccg ccaataattg tttgccccga actttcgtcc gtgtgaacct
      841 taaaggttgg gtcttcctcc gctaaacgtt gcagcgccaa cgccatttta tcctggtctg
      901 cggtagattt tggctcaacc gcaatcgaga tgaccggttc agggaaggta atgctttcaa
      961 ggattaaggg tttgttaacg ctgcagagag tgtctcctgt aaaggactct ttcagcccca
     1021 acactgcgcc gatatctccc gcggtaacct cggtgatgtc ctcgcggcgg tcggcgtaca
     1081 tacgcagcaa tcgaccgatg cgttccctgc ggcctttaat cgggttcaac accgtatccc
     1141 cggttttgag cgtgcctgaa taaaccctga agtaagccag gcggccaaca taagggtcgc
     1201 tgacaatctt gaacaccagc gcgctcaatg gcgcgttgtc ttctgttggc agctccattt
     1261 ctttttcacc gtcaggcgaa tgcgcgataa cgggggggat atctacagga gacggcaggt
     1321 aatccacgat ggcgtccaga acaagctgaa cgcctttgtt gcgtaatgag gagccacaaa
     1381 atacaggtgt cgcgctggtg tttatgactg ccgcgcgcaa agcctgtttt aactcatcga
     1441 tagagatttt ttcaccttcg aggtacttca ccgttaagcc gtcgtttaat tctacgattt
     1501 gctccaccat ttgctcacgc aggtcttcgg cttgcttgcg gaggtcctct ggaatggggg
     1561 cgtacttggg ctctttgcca aggtcatctt caaaatagac cgcttgcatc gttaaaagat
     1621 cgaccacacc tttaaaaccg gcttcgctgc cgataggcag ctgcatctga attggctgcg
     1681 cgcccagtct ttcgcggatc gtgcccaaag tgtattccaa ggacgcgcca acgcggtcca
     1741 ttttattcac gaaacaaata cggggcacac tgtatcggtc agcctgacgc cagacggttt
     1801 cgctctgcgg ctcaacgccc tgaacagcat caaacacaac caccccgcca tccaacacgc
     1861 gcagcgagcg ctgcacttca gcggtaaagt ctatgtgtcc cggagtatca atcacattga
     1921 tctgatatcc cttccattcc gcggttaccg cggcagaaac gatcgtaatc ccacgctccc
     1981 gttcttgaac catccaatcg gtaactgtgg ttccttcatc cacgtttccc agccgatagg
     2041 ttctgccgga atagaagagg atacgctcag tcgtcgtggt tttacccgcg tcgatatgag
     2101 caataatccc aatatttcgg cacttgtcta acggaaaatt gcgtgacatc gtttcctggt
     2161 atcagaaatg gtttacatcc ggtaatggga aaaggcgcgg ttagcctcag ccattttatg
     2221 agtctcttcg cgcttgcgca ctgcgttacc ctggttattt gaagcttcga tcagttcagt
     2281 cgagagcttt tccgcgtagg aattgccggt gcggctcgtc gcggcatcca gaatccagcg
     2341 catggccagc gtcatacgtc ggtgcgcggg aacctccata ggcacctggt aagtggcacc
     2401 gcccacacgc cgcgggcgta cttccatcac aggaccaacg tttttaagcg caaggtccag
     2461 cacttccaca gggctcttgc cggttttctc tttaatcatt tccagcgcat catacataat
     2521 agtggtggca acactctttt tgccgcgttt catgatgcgg tggatgaaat aggtcacggg
     2581 aacgctgtta taacgcacat ccggggtgac ttcacgtggt tcgggttttg atcttcgcat
     2641 gcaaatactc catcaaatcc tatggttatg ctttcggttt ctttgaaccg tatttcgatc
     2701 gcgcctgtga acgtttcgcg accccagagg tgtccagcgt gccgcgcaca atgtggtagc
     2761 gcacaccagg caggtctttt acacgaccgc ctcgaacgag caccaccgag tgttcctgca
     2821 gctgatgacc ttcgccaggg atataggcag tgacctcgat cccgtttgaa agcctgacgc
     2881 gggcgatctt tcgcagcgct gagttcggtt tcttgggggt catcgtcctc accactgtgc
     2941 acacgccacg cttctgcgga gcgccttttt caaggcggga acggcgttgc ttcattgagt
     3001 ttaaagtgta ctgcaacgca ggcgccttgc ttttggccct tttcgtctca cgaccttttc
     3061 tgatcaactg attgattgtt ggcacttctt ctttgcctcc gtaaatttga tattgcctgt
     3121 tcttttctaa ttaagatgag accatcgact gcaatgatgg cctcaccggt tcacactgat
     3181 ggcagaggtg cacacatcac cacgtaacta gagcaacggc gaaaaattat aacacgaggg
     3241 tttttcatgg tcaagagaaa tccataaaaa acggcccgcg actgccctgt tacagcagtc
     3301 cctgcaactc ctcgccgagg cccagtaacc cggtttcaag gcgtggaggt cgtacacgtt
     3361 cttcatcttt tccaaagcgg atgatttctt tggtgtccat caccgcttca acagccaaac
     3421 ccaggctttg cagctctttc accaaaactc tgaaggaagc cggtaggctg ggatcttcta
     3481 tgggctcacc tttgacaatt gactcgtagg tgttcacgcg accctgaaca tcatccgatt
     3541 tgaccgtaag catctcttgc agcgtataag ccgcgccata tgcttccagc gcccaaactt
     3601 ccatttcacc gaaacgctgt ccgccaaact gcgccttgcc gccaagaggt tgctgtgtca
     3661 ccaggctata cgggccggtt gaacgggcat gcaccttgtc ttctaccagg tgatgcagtt
     3721 tcaacatggt cattacgcca acggtaaccg gctggtcata agcctggcct gttttgccgt
     3781 cgcgtaatat ctgctttccg agcgtgggaa ccggctgccg ggtttcaagc gcaactttat
     3841 ctgccacttc tcgaattttc tcgtctttta tttttgacgg gtctgtgcca tttacctcaa
     3901 gccaaacacg gaggcaggtg ttaatcgcct gggtgtctcg ttctttcctt tctgcttccg
     3961 tgttggggtc agcagaatca agaatggggg caaggtcgaa gcctctttca ttaagccagg
     4021 attccaacgt gatctggcgg gcgtaggttg ggtccgtggc aatgcggccc atgtcgtact
     4081 tgtggtcgag ggtctgctcc aggtacagca gccgcacttc gtcgtcatct ttaatcgatt
     4141 cagggtcgtg gtcaatgccg ttcagccact cccaggctct ttttcccgct tctgtccatg
     4201 tgtaatccat caaccaggct ctggcgagtt cagcttcaat ttcgtattca ttggcgccgt
     4261 caaatactgg cgtaatcgct tcaaaaccca gttctttggc cgcccaaccc aggtgcgttt
     4321 ccagcacctg gccgatattc atacggcccg gcacccccag cggattgaga atgatatcca
     4381 ctggcgtgcc atctttaaga aacggcatat tttccaccgg aacaacgcga gaaacaacac
     4441 ccttgttacc atggcgtcct gccattttgt cgccggcggt gatcttacgg cgctgcgcga
     4501 ccgagacgcg caccatcata tccactccag cggagaggtc cacattatcc tcccgggtga
     4561 agactttcac atccacaact tttccgcgct ctccgtgggg catgcgcagc gaggtgtcct
     4621 tcacatctcg agatttttct ccgaaaatgg cgcgcagcag gcgctcttcg ggagttaatt
     4681 ctttttcacc tttgggagtg atcttgccga ctagaatatc gttagggcct acctccgcgc
     4741 cgatgcggac gattccctgt tcatccaggt ctttaaccgc atcctcaccc acattgggaa
     4801 tgtcgcgggt aatttcctcc ggtcctagct tggtatcacg cgcttccact tcatattttt
     4861 caatgtgcac tgaagtgaac ttatcctctt gcaccagtct ttcagaaatg aggatggcgt
     4921 cttcaaaatt cccgccttcc caggaaagga aagccaccac cacgttctga cccaacgcca
     4981 gcttgccccc gacagtggag gaagaatctg ccaggatatc tcccttgcga atacgctggc
     5041 ctttgaccac agccgggcgc tggtcaatac aggtgctctg gttggaacgc tgatacttgc
     5101 gtagatggta cacgtcattt tttccttcag cgtttttcac aacgatttgt tgcccggtga
     5161 ccgaaatcac atcgccgtct tcatgagcaa ccacaacctg accagaatcg agcgcggcgt
     5221 aacgctccat gccggtagaa atcatggggc tgtctgggtt gacaagcggc accgcctgag
     5281 cctgcatgtt cgagcccatt aatgcacggt tcgcgtcatc atgctccagg aaaggaatca
     5341 acgcggcgga aatacccacc acctgatgcg gcgagacatc catgtattga acgctttcag
     5401 gggttgaaag aataaaatca ctgtgatgcc ggcaggaaat ctggttctgc gcgaattcgt
     5461 tgttttcatt caaaatagtg ttcgcctgcg cgatcacgta tttatcttcc gcgtcagccg
     5521 acaggtattc aacctcttca gtaacaaaag gtgcaacgtg aatctcttct tttttcgtct
     5581 tcttaatcgc tgaaagcatc ttggcggtaa cgcgctcacc ctccggggcg aagacctcgc
     5641 ctgatttttc atccaccagg ctctcgcgca ataaccgtcc tgcaagcctg gggtcatcat
     5701 atgccaacgt ttttaatacc ttacgataag gggtttcgat gaacccgtat tcattcacgc
     5761 gcgcgtaact cgccagacgc ccgataagcc cgatgttcgg gccttcaggt gtttcaatgg
     5821 gacaaatacg cccatagtgc gagtggtgaa catctcgcac gtcaaagcca gcgcgctcgc
     5881 ggcgcaagcc gccagggccc agggcagaga gcgtgcgttt gctgcgtaac tcagccagtg
     5941 ggttggtctg gtccataaac tgcgagagtt gtgaagagcc gaagaactct ctcagcgcgg
     6001 cgaccaccgg gcggatgttg atgaggctga caggtgaaac ctggtcctga tcgcggattg
     6061 acattctttc tttgatcacc cgctccatcc ggcgcagccc gatgcgcagt ttattttgta
     6121 tcagttcgcc attggtcttt acccgacggt tccccaggtg gtcgatatca tccttgtcct
     6181 ctatttcatt gttgatcaga atcatccggc ggacaaggta gacgatatcc cacttggtca
     6241 cactgcggtg agaaaccggg atgcggctca tcaggtcaag cttctggttc aacttatagc
     6301 ggccaactcg ttcaaggtca taatggcggc tgtcaaacag ttgctcttgg agaaattgcc
     6361 tggcattctc aaggttagcc gggtcgccag ggcgcatacg tttaaagaac tcaatcagcg
     6421 cagcctctgc aatcgtctga taatgcgtca gatcccattc aggttcctgg cggatagtgg
     6481 aagggataaa ttgccggtct gggttattgt cgatgtcact gaacaatgca atcagctcct
     6541 catcagagcc agttttgacc ggtgattcac caggaagcag atcatcaacc gctgccagcg
     6601 cgcgtaagaa aatcgtgata ggaacggtgc gcttgcggtt gaactttaaa atcaaataat
     6661 cattcttgcg cgtctcaaac tccatccagg caccgcggtc tgggataagc ttggccatcg
     6721 ccaggcgatg accggtagcc cggtctaccg gggcttcaaa gtaaacgccc ggcgaacgaa
     6781 taagctgcga tacaaccaca cgttcagtac cattgatgat gaaggttcct ttttcggtca
     6841 tctcggggaa atcgcccagg aagatatctg acttcaccgg ttctgggata tcagggccag
     6901 ccagaagcac ggaaacgtac agcgggctgg cgtacgtcaa gtcacgttcg acacactctt
     6961 caatggaatg cttgggcggt tcaaaccagt atttcagtcc ccattgtttc gcctcagggc
     7021 tgcggccggg gaagtacagt ttcatcccct tgttatagga ttcgattggc gagatctcgt
     7081 gaaagagatc ctccagccct tcttccttta atcttatgaa agattcaagt tggacttcaa
     7141 tcaaattggg caggtcaaag atggttggta ttcttgcgta atttttagtg ggcaacacag
     7201 aagccattgg tttctcctaa aatgtcaaaa ccgacgataa tcgaataaac tcccagccca
     7261 ttccagagcc gggatgcgca acttgtcatt ataccaacgc gtttttcaat ggtcaaggaa
     7321 ttcgtgttag ttcatttagg ggggtcagca catcgtctac agtttatatg cgctttgcct
     7381 ttgcgtatta acgcgtattt cacctctccc gctcccgtcg gctgatcacg ggtgaccctc
     7441 aagccaaaca atttctgctt tcacctcagc ctgaggggag acacttatca cgccaaacga
     7501 cagccggtaa tcaggttttt ccgctacgct gcacgaaccc gggttaaaga acaaaacatg
     7561 ctcccgcagt tggttctctg catggtgtga atgaccgaag atgaccacgt ctgcgttcac
     7621 tgccaacgaa tggagtcgcc tgtagtaacg agcaaactgg taaccgtgca aggtattttc
     7681 cagcttatca cgccagtaac ctgccacgcc caggtgcccg tgggtgacaa cgacccgggc
     7741 accatttaaa ttgagccgct gcatcacggg cagtgaacgg agaaagtaaa ggtcgcggtt
     7801 tcctctcaca gaatacaccg gcgccaattc gcccagctgc tccagaacac gagggtgtga
     7861 aatatcaccg gcgtggatga tgaggtccgc catgctgttc ttaatcaatg ggatcaggtg
     7921 tggatgcagt ctgcccacac gatcgatcac atgtgtatcc gccagcaccg ccgcgacaat
     7981 ccttttttct gattgaattc gtatttcctc agggtcagac aagtttgctt tccacatccg
     8041 ccatcgcctg ttctgaattt tcaaaaacga ttccgtgcat acccaaccag ttggcggtat
     8101 caatattttc ttgcaggtca tccacaaaaa ccgcttgata tggctcagct cccaagcgtt
     8161 tgagtacatc caaatagatt gcggggtcgg gtttcgccat cttcacttca gccgaaatga
     8221 cgatgtggtc aaaaacatgc aggcaatcaa aataggttgt caaggccttg cgcgcgccag
     8281 accaggcgtt gcttagcaac gcggtctggt aactgccgcg taacttctgg atgaactcaa
     8341 ttaaccggtc atccacccgg gtgccggtcc aaaattgaag ctgaaaatcg cgtaaatcag
     8401 caggcgggac gccgagggtt ttccctacct gttgccagtg ttcctcctcg gttatcgcgc
     8461 cgagggtcgc aaggcgggct gtttcactgt tgaaaaccag tttatccagt tcacccaggg
     8521 tcacgctgaa tttctttgcc agttcctgcc gcggacggtc gtcgaccagc tgcaacagca
     8581 ctccccccat atcccaaatg acggctttga tcgtaccagg catttttctc ctctttatta
     8641 gatattgaaa aagagtataa cacgggaaaa tcggagagct gcgggaacaa cgtaacaacc
     8701 tctatctcca attgttcact cgttcgccac gtttttcctg gtttattcca acgagaaccg
     8761 ttataacaat gcccaatcgc gcaggaagac tcccaggtca atcaagccca ggtgctcatt
     8821 taggaaaagt cacggaaccg cattgcaccc gtaaccactg agcgcagtga tatggcggct
     8881 atccaatagc aggcgcatct caattcgaaa agacagcatc gtctgcgctg cgaagcacgc
     8941 aaaaaaatgg tttgtttgca gggtgataaa ccatactggc acgcacatct cttgaggttt
     9001 ttttggcact tgcagttgcc ttctctttta agtgacaaca ctcctaaatg cctgagcgcc
     9061 ttcactgaag aggtatgtta ctgttctaaa tcaataacca ttcaatctct tgaatatttg
     9121 acagcctgat aatgatcctc ctcaatagtc agccttcagc tcaccagtta aggctttcat
     9181 atagttgtat aaccttatcc gcgtaccagt aatccacgtc ggcagggccg taggatttca
     9241 atccatcgcg agcgttcccc gcgcgctcga tcaaaccagc cagcatacgt gttccgtaca
     9301 tcacgttaaa atcagggtct ttcaattcag caatagtggg cctggaggaa aaacaaggac
     9361 cgttaatgca ctggaaaccc gcagcgatgc cgtcgctggg catcacctgc atcagcccca
     9421 ccgcgccaga aacggagata acctccggct gcccgccgga ttcaatcgcc atcaccgccg
     9481 ctatcagcaa cgggtctata ccatacgttt gcgcgttggc ttcaatcagc ccacaccact
     9541 caaccacccc tgcgggataa cgatcatgaa attgacaggc ttggggggaa atctctgcgc
     9601 cagtagcctg gtcggtctcc atggtcctta agggttctgc tgcggcgcta gctgcgccaa
     9661 tcggttttac cagttgctgc agtaagaaca gaagcaggca actgaaaatg atgccaggaa
     9721 ccagcgcctt ttgcgtgcca atcataatga acctccgctg aactttttct ttatgatagc
     9781 acttttgttc tattttgtca aaattaaaaa ggcgggggtt cccgcctggc ggtgctcatc
     9841 tttccatgct gacaacctgg tgatcccagc ggtgccagcc gttgaacctc tccgtcaata
     9901 tcttcttgcc ttctcccggg ctcttttgcc cgctcaactc caggtcggca tccaccatga
     9961 tacgtaccag ttcagagaac ttgatcttgg gctcccaccc cagcctggcg cgcgcttttg
    10021 ccggatccga aagcagatag tcaacttcgg ttggtctgaa gtagcgtggg tcgatctcca
    10081 cgtatttctt ccaatccatg cccacatagc caaaggcttc ttctaaaaac tcgcgcgggc
    10141 tgtgcgcttc gcccgtaccg atgacgtaat catcaggggt gtcctgttgc agcatcagcc
    10201 acatggcttc aacgtattca ggcgcgtaac cccagtctcg ttttgaatcc agattgccca
    10261 ggtagagctt actctgcgta cccgctttga tcgcagccag cgcgcgggtt atcttacggg
    10321 tgacaaaggt ttcgccgcgt cgcggggatt catgattgaa aagtatgcca ttggcagcga
    10381 aaatcccata accttcgcga tagttgcgcg tgacccagta agaatacacc ttggctgccg
    10441 cgtaagggct gcgcggctca aacggggtta attcattttg cggcggtttg gcagccccga
    10501 acatttcgct ggaggacgcc tggtagaacc tttccgggtt gccgctgcgg cggatggctt
    10561 caagcaggcg aattgtgccc aatccggtca catccccggt gtattccggc atgtcaaaac
    10621 ttacccgcac gtggctctgc gccgccaaat tatagacctc gtctgggcga acattataga
    10681 tcacgtccat caggctctct atagccgcaa tgtctccgta atgtagatag agcttcacct
    10741 cttcaccgtt tcgatgcgga tcatggtaga tgtgatcaat acggcgggtg ttgaaggtgc
    10801 tcgccctgcg gatgataccg tgaacttcgt atccttttga gagcaacagc tcagccaggt
    10861 aggaaccatc ctgcccggtg atgccagtga tcaatgcctt tttcatgctt cgcctccatg
    10921 gcgtcgagtt attattcttg ctctatctgg cttgccagtg ggtaattata accgaaagaa
    10981 agtcggtttc tttgtggtaa gccgcctggc gcgccatcag ctttcagatg ctttttaaat
    11041 agaaagcccc gggtctctat cacctgcccg gggctttttc ggttgtcact tttcttgctt
    11101 acggaaagtg atttcttgtt tttctttgtc cagatccacc aacacggtgt caccgtcccc
    11161 atattcacct gaaagcaatg ttaccgatag cggattttct acgtatttct gcagcgctcg
    11221 tttcagtggg cgggcgccaa acgaaggatc gtagccttct ttggcgagcc aggtgcgcgc
    11281 ttcgtcagtc aattccacct tcagcccgcg ctcttctagc cgggatcgca cttcattcat
    11341 ctgcaggtcc acgatctcca gcatctgctc aggtgtaagc gctgagaaca tgataatctc
    11401 gtcgatgcgg ttcagaaatt ccggcctgaa agtgctcttg agcgcttttt cgatcttttc
    11461 ctgcgcctga cggtcctcat ccgtatccga tttctgtaaa aagccaagcg ctccaccctg
    11521 tcggacaaat tcagttccca gattgctggt cataatgagc accgtgttgc ggaagtcaac
    11581 caccctgccc tgcccatccg tcagtcgtcc atcatccaga atctgcagca atgagttcca
    11641 cacttctgga tgggcctttt caatttcatc aaacaggatt acccggtatg ggcggcggcg
    11701 aaccgcctcg gtaagttgac caccctcctc ataacccaca taacccggag gggcaccgaa
    11761 caggcgtgaa accgtatgtt gttcacgata ctcgctcatg tccaggcgaa ccagcgcgtc
    11821 ttcatcatca aacatgaact cagccaacgc ttttgccagt tcagttttac cgacacccga
    11881 tggaccaatg aaaatgaatg atccgatcgg gcggcgggga tcttttagcc ctgaacgcgc
    11941 ccgccggatc gcatccgata ccgcgcgaat ggcctcatcc tgcccgatga tgcgctcatg
    12001 caggcgatct tccatcttca gcagtctctc cgattcttct tccagcatct ggctaaccgg
    12061 gataccagtc cactgcgcca gcacctgggc gatgtcatcc acatccacca cttcatccag
    12121 tttatggtct gattcccatt tatcccggtc ttggttgaat tgttcttcaa ggcgcaggcg
    12181 ctcagccttc atgcgcgcgg cgcgctcgta atcacgctct acacccgcca gctcttcttc
    12241 ggctttcaac cgttcaattt cagacttggc ttgtttcagc tcaggcggca tagagtacaa
    12301 cgccactctt aatttcgctg ccgcttcatc catcaggtca atcgccttgt ctggcagacg
    12361 cctgtcgctg acgtaacgcg aactcagttt tactgccgct gaaagcgctt catcagaaaa
    12421 gtgcaccttg tgatgtgcct cgtaccgatc gcgcagcccc atcagcattt tcaacgtatc
    12481 ttctggagaa ggctcgtcta caaacaccgg ggcaaagcgc ctttctagag ccgagtcttt
    12541 ttcgatatac ttttgatatt catccagcgt agtagcaccc acacattgca gttcgccacg
    12601 cgacagcgcc ggtttgagca tgtttgaagc gtccatggcg ccctgggcag cccccgctcc
    12661 aacaacggtg tgcagctcat caataaacag gatgatctcg ccggaggaac gctgtacttc
    12721 ttcgattgac gctttaagcc tttcttcgaa ctcgccgcga aaacgcgaac ccgcgatcat
    12781 tgcaccaaga tcaagagaga ttacgcgctt ccccattaaa atttcaggca catcattgct
    12841 ggcgattttt tgcgccaacc cttctacaat ggctgtttta cccacacctg cttcaccaat
    12901 caacaccggg ttgtttttag tgcgccggga gagaatttgc atcacacgca aaatctcatt
    12961 atcgcgacca ataacggggt caagtttgcc ttctttcgcc tgctgtgtca ggtcgcgaga
    13021 gtatttatcc agtgtgcggt aacgactttc ggtctgagga tcggtaacac gttgcccgcc
    13081 gcgcagttca acaatggctt ccattacccg gtcacgggtg aggcctgtcg tttcaagtag
    13141 cttggcagcc ggcgtattcc tttcactcag tatcgccagg aacaggtgtt cggttgagat
    13201 gtattcatct ttcaatttgc tggcttcttc attcgccagg tccatgatcc tttttacgcg
    13261 cggggtgatg aatacctgcc cggcgccctg accgtaaata ctggcttttg ggctggtttt
    13321 aaggatataa tccagccttt cttccaggat ttgcggatcc acttttataa gagccaggat
    13381 ttgaggtact actccttgcg gttgttcgat cagcgccaac aggatatgct cagtatcaat
    13441 ctgattgtgt ccatagcgct gggtgatctc ggcagccctc tgagcagcct cctgcgctct
    13501 ttctgtaaac ctatcaaatc gcatcataat tcactccgaa tcaatcgtcc ttcctcgagg
    13561 tcattatagc atcgaggata tcaaacaaat caaagatttt tataagaatt cggttaggct
    13621 ttttcaccca tagtaattcg cattgttctg tgcagccctg atagaaccag gtgcaaggtt
    13681 cacgccgttc tttaaagaca gtgtaaaatt tatggcatgg atgaacgcaa gacagaccca
    13741 cgaatccggc caattgaaat ccagcgcgac ttgccagcga tcgctgacct gatcgagacc
    13801 gctttcgccg agcagatgga tgatgagggc agagactatc ttcgccacct ccgtcacatc
    13861 gcggcaggag ccggcgcgtt catcctggac ggaagtacac ccgagagctc taccctgcct
    13921 ttccacggct acttgtggga ggagaacggc cgcgttatcg gcaacctcac gctcattccc
    13981 ctccgaaaaa gagaccataa atcttacatt atcgccaatg tatctgtata tcccgagtgg
    14041 cgcgggaaag gtattgcccg ccaactgacc gatagagcca ttgcccatgt gcgtgaacat
    14101 gatggtcggc atatttacct gcaggtgagg gcagataacc cgcctgccat tcatatttat
    14161 caacacgcag gttttgaaga attcacccgg cgcacgtcct gggtcttttc aaaaaacaat
    14221 cttcaccctc caatccttcc gccgggggtt gatgtgagaa gaagaaaacg cgcggactgg
    14281 gcgcagcaga aagactggct gcagctcact tacccgccag agatcacctg gaacctgcct
    14341 tacagcctgc cgcgtctgcg ccctgatttc tggtcttggg ttgaaaacct gttgagcgcg
    14401 cgtgtgtgcc gcacctggtc agcccaaaaa gccggccgct tgatcggcag tgcctcctac
    14461 gagaacggtt ataccggctc tgattaccta tggctggcga gttcgccggc atgggaagat
    14521 atcgcgattc aggcgttact gccacatgta tacaccaggg tattgaagcc gcaacgcatc
    14581 gtggtaaatt accccgcggg cagaggaata aacggattta ccgcctgcgg catgaaagaa
    14641 ctacacaccc taatctggat gaaaatggag attccctcca ggttgtacag tctggatgga
    14701 tgaaactttt ttccctcgcc tgcgtatgta tgggcgaaag gaaactcata tgaaaagatt
    14761 actgctgaag gtactcatca acgctggcgc tcttttcctc gctgtgtggt tattgcgtcc
    14821 gcatattgtg atgcaaaatg atgcctggta tgcgtacatc gtcctcggtg caatattcgg
    14881 tcttgtcaac gcgctcatta aacccgtggt aatgttcgtg agttgcccgc ttgtggttct
    14941 ttcgctcggg ctggcaacac tgctggtgaa cacgttgatg ttccttttcg ccggctggtt
    15001 tggtacggtc ctcaacatcg gcttcaccat tccgcagcag caattctatt acgcgtttct
    15061 tggagcgctt atcgtcagcg tggtcagctt cattctcagc cgcattctag acagttctca
    15121 ataggctgat cttcatcatg ataaaaacag cgccagtgag gcgctgttaa tttttcgttt
    15181 cttatccatt aaggaacagg gtcagccgga acccacttaa aacctgtcgc gtcccattta
    15241 tattgcgttt tcccatcttt ccgcagcagc cagtcaccat tggcatcttt ctctggtaag
    15301 acctctacag ccagttgagc agagatactt tctggaatca ggggcaccca ctctgttcca
    15361 tcgtcgctta gccgataaac cgggatttca acataattca ccaccagccc gctctctgct
    15421 tgataagata gcccacgcgg aatctgcggg aggctggatt cagatggaaa ttcaacatcg
    15481 ccctcctcga cagggatcgc gagtggcggc aggagaaaac caagggtgaa gttattttcg
    15541 gcatcgctca cccaaacccg ttcatcacca gccacggcga tgccactggg tacgccaaac
    15601 ccatcgatgc ccgagctaac ctctccccag ccgcgcaaga agttgcccat ctggtcgaac
    15661 tccagcacac gaaagccgtc cgggtctgtc acaaaaatat ggttttccgc gtccagcgcc
    15721 aggaacggtt tattgttgat ggactgccca aaccagccat gcacatccca ggacctgagc
    15781 acgctgtaac cttctttgcc ggcgggttca aaaacctgga tgcgctgatt ccaggtatcg
    15841 gccacataaa cccgtccgtt gccatccacc gcgataccga ctggttcatc gaactcaccg
    15901 gggttgatgc cgctgcggcc aaactgttgc tggtaattcc cgttttcatc aaaaatcacc
    15961 acgcggttat tacccgtatc tgtcacatac accgtccctt cgcgatccac cgcgatgccg
    16021 cgcggacccc agaagcggtc cggttcttcc gccaagccgg gaactcccca catggtgagg
    16081 aatttgccat cacgggtaaa cttctgaatg cggtgattcc aggtatccgc cacgtacact
    16141 gagccatcgg gggcaactgc cacgccccac ggctcattga aggtaccgcc tggggcgttg
    16201 ccgccttccg ctgtggcgta acttccccac gagagcagaa acgcgccatc cgcgctgaat
    16261 ttttgaatgc ggtgattatt ggcgtctgcc acataaaccg atccatccgc tgccaccgcc
    16321 aaccctcgcg gcatggtcag ttgtccttca gcgctgccgc tgctgccaaa aaccctatca
    16381 ggcgttaatt caaaatactt ccccgcgtac gggtcggttt ctgctgtttc ctgctgctgg
    16441 gggatagcgc catacgtcca gatgcgctcg acaatgtcct tttggataaa aaggtagata
    16501 tttgccgaag gctgccagtt ctccagcgtc aaaccactat tattggcaag cgacgcgtac
    16561 agtcggtaat ctttgttcag ccagatatta ataattgcct ggcgcatctg gggattggtg
    16621 aaacccttcc agaaccgttc ccaggtcata ttgaagtaat cttgcatcgg ccagaccagc
    16681 cgcttgtatt tggtttcaaa atactggtct ttcagtatgg cctgagtttt tgaataatgc
    16741 tcatcgtcag agataaccac cgggtaattc aacaggtccc gcgtcaggtc atccttcagc
    16801 cagaccttgt tgggataatc gcggaagtac caccagtaag ggtagagcgc gtccccgata
    16861 taagccacgc ggatgtcctt ccctccagtg gtgcgctgcg aaatatcctc cacctgctcc
    16921 agcacatcct tcggtcccct ggcggcgtgt gcgtaaacga gaaactcttt gccagtgtca
    16981 tagttgataa aactcgcctg gtacgcggag cgcgcggtaa gcaccatcag gatcgcgctc
    17041 aaagtcagcg tcaataactt gagaatcgcc cgctggctcc agcgcagcag caatttccac
    17101 aagagcccgc cagatgccag agtggcgatc aaggcaaaaa ggaaggtgct cgttgccttc
    17161 aactgctcca gctctttgcc ggcgaagggt ttttgggtgc ccaccagcgc ggcaatcgtc
    17221 ccgccgaggc tggtgatgaa caccggcagc agcgcaagga caagcagccc ttttttgttg
    17281 gccagttgtt tgaagggagt ggaatccacc aggtagccaa agccaaaccc ggcgcacaat
    17341 aacaaaggca gggttacgtg cacggtcagc cagggcattt tttcacctgc gagcgtgaat
    17401 gccaccaggc tggtcaatcc ccagaacacc aataaactca acaccggtaa tttgcctggc
    17461 ttttctgctg gtgtttcttc actgccttcc agtgctggca gtgggagctg actgggaacc
    17521 tcaacctgct ccgcggtttg aagcgccggc gcaacaccgg gcaggctgga aaaaagcctg
    17581 tgcttgaccc caatgacgac cgcgattagc gtacccacca acggcaggta ttcgtacatg
    17641 ggcacctgca gaaaggcgaa aaaataccag ggttgcgaac cacggttaac cccctgttgg
    17701 ctcagccagt aacccaacga tcctaccatc ccggtgaaaa atccctgccc gttggtgaaa
    17761 aaagttgtgt aaaaaatcgt gaagatggca tagaaaacac ccacgttgat cagccacagg
    17821 cgcggtttcc agagcaatcc cagcgtcact gaaatgacca ctgctcccag caagaataac
    17881 aaggtgcgaa tcatccctgt ttgagaataa tccagcgggt tccaacccag caggttgata
    17941 ggaaaagcga tcaattgcgg caggatcatt gtggaggtca gaatgagcag atcaaaagaa
    18001 cggatctccc tgaccacgcg ccagcccatc gacccaagca agaccacaat ggccaagatc
    18061 atgcccagcg ccgccagcgc caggctgatt aacatgactg tgtgatacac cgcaggagca
    18121 gccacagcct ccgcgttacc aggtttgata ctggcatcca tcaccgccgc gcccaacgcg
    18181 gtaccaacaa agagcagccc tgccatcatt gaaaaaatga agaaatcgcg ccttcctcct
    18241 tgcggccatt catgcttcac cacgtctcga atgaacagca ccgccaggaa gatgaggatt
    18301 tgggcggtgt aaataaaagc ggtttccttc gcggtgaaat gaagcgccag agccactgta
    18361 aataagtaga gatggcggtg gcggccgtct tccaggtaac gcagcacggc gtaaagcatc
    18421 acgacgccga acaacgccac aaaagcctcg ttacgcgtgt agcgcccata gaacagcatg
    18481 taaggtgata ccaggaagaa ggcgcctgcc agcagcgcgc caaccctgcc caggtacctg
    18541 cggaatccaa atatcacaaa aatgatcgtg gcaatactga acaaagccgc cgggatgcgc
    18601 gaagaaaaat cagaatcgcc aaaaaggaaa taggagcccg ccaccaggtg aaattgcagc
    18661 ggaccatgcg tcaccgggtc gtgtgcgtac ccccgcccct gataaagttc atacgaaggc
    18721 accacatggt taacctcgtc atggctcatc acgcgtaaac caactgcgta aaaacgagac
    18781 agcacagcca tcaggatgat cagcgcaatt acaatgtgtt caatggtaat tctgggtatc
    18841 caggtgtaga gcggacggtc taaccagccg ggtttgcttt ccacattttc agcgctgttc
    18901 atattcaaaa ctgatcctct gtctgcaaaa aatatacctc acaacatttg tttcagaaat
    18961 tactgacaag acgtgttttt cattcgcttt ttttttataa cgtctgaaac aatatccagt
    19021 tcattctgct gttaaattgt aataattgta aaccgccctt gacaggtctg cgaatagctg
    19081 gttcgcatta tcccaaacga tctggttctg gtcgtaaatg aagatcacca gcacataatt
    19141 cccgccaggg gtgtacacca ggccagcatc acccaccgta tgcattacgc catccattgc
    19201 atcaattgcc cagccatgtt tgtgcccgat ctgggtgccg tccggaagcc cggcttcaat
    19261 taacacgcca attcggttct gtgtgagtaa gctgatcatc gaccggcatt cagcgggggt
    19321 aatttcaccc gggaataccg ccaccaggct gccgccgtca ttctgcgcgc actgatagat
    19381 atccgccaat aacaaaccca tttcagtagg ggtggtctgg ttatatggat cagggtcggt
    19441 aaaaacgtca cgccgagaat tagcgggggt gacaatgttt cctgtaagca gcggcgcgcc
    19501 aatgtaaaac atgccatcca gaaaagtgct ttgcagtccc agtttgcgca tcgtgctggt
    19561 cacagtcagt ggaccaaggg ttggcgagag gacttcacgc atcaggttgt ctgtagaggt
    19621 gttgtctgac atggcaatca tagactggag cagttgtacc actttcgtgc tggcaggttc
    19681 agccacctct tggtaggcgg tgaccattac ggcgattttg atagtgctat ccgctgaaaa
    19741 cgctatgcca ggagtgaggg attcgccatt ctggtaagcc atttgcatct cgctaccgtt
    19801 tttcaagtcc tgcaggtaaa gttccaccac gccgtcaaaa ttattcacat ctacaatttg
    19861 ctgcaacagg atacgtagat tatccataga agggcggttc agcttaccgg taatcagcgc
    19921 gaggttgacg atacgtgcag caggagagcg cagcgcgttc gataccagca gcgcagaacg
    19981 gtccacatcc agtttcttgc ctggcgtgcc ggcttcaaag gtgacgctcc ccggcacagg
    20041 taaataagcc gcaggttctt catcgtaacg gctggcgatc tcattcacca ggtaagcgcg
    20101 cagctgcttg tcatcgattt ctgctcgcaa tggtatctcg ctggccgaag gctgctggtt
    20161 aaaaaggtaa gcgatgaagg acggccagaa aggttccgcc gagcgcaact gatctgccgc
    20221 ggtcatcatc gcagtcatat ctaaagaaaa tcccagcgtg ccaggcttaa cttggatgac
    20281 gctgttgtta taacgcagtt ctactggaat ggaatacgcc tgtgtcacgc gtgaagctgc
    20341 ctgttccatc gacaagccgg taacatccac acctgcaatg ctggttcccg tggaaaaaga
    20401 tgagcgcaac cggctgaaaa ccacaagttg aaatacggtc aacaccagcg cagccaaaat
    20461 aaaagccagc gacagccagc gaaatatcga aaaatgccca acgcgcttca tcattaaatc
    20521 agtttccgta catgccatgg aaattttacc agactttggc accccccttt ttgctgaaaa
    20581 accacgcttt tttttgagct ttagttatac ttatagtatg gaaacagttg agcctgcaac
    20641 ggtttcgctt ttagcggggt tatttcccct cacattgttg gaagggaacg accttagcca
    20701 ggtggctgcc caatttgagg tactgaatta cccgcgcggc agccggctat acgctgctga
    20761 tgatattcca gattattttt attggatcat ttccggtaat gtacggctgg cagggggcgc
    20821 catgcggcgc ggtaaagctg agcaggtgct gggcgccggc gattatttcg gcgcagaagt
    20881 gctttctcgc agcgattatc gcttttcaga tgctgtctgt ctcagcgagg taaagctggt
    20941 cagagttaat gagcaagacc tcattcagtt gtgcgctaac cttccaacca tagaaaacgc
    21001 gttttcgctg atctaccaga cccacaaatt ccgcgcgcgc ctctttttcc cctggctgga
    21061 gaaaggggaa aaagtcaacc tggtttgccg tcggcaccca ttctttctcg cactgcgcgt
    21121 ggtactcatt ggcggcgcgg taattgcaag tttcgctggt ttgctatcgc tggcgctgtc
    21181 cagcaaatcg gtggccttaa tcctgctcgc cggcacgctg ctcttgatcg gcactttgct
    21241 gaccgcgtgg tctgccctgg aatggggcaa tgactttttt atactgactt ctgaccgggt
    21301 gctcgtgcaa aaaaaactaa ctggtttttt cgacaaccgc caggaagctc cctacactgc
    21361 catcctttct accggctttg aaacatcttt ctggggcagg ctactgagat tcggcacgat
    21421 caacttgaga acctacaatg gtaagctcag ctttaaacgt ctgcctgtcc ccgagattat
    21481 tttcgatttg ctggaatatc aacgcaggag cattgcccag aaaaagcatc agagcgatta
    21541 cgctgaaatg cgcgaaacct tgcagcatcg gctggaagga gcaacggtca ggacgaaaaa
    21601 cgctgccgct ggtaagcata caggaggatt aaaaacgact tataaaagcg gttcttttct
    21661 ggatctggca gcgcggtttt ttggattgcg ccacgagagc gcgggggcga ttatttaccg
    21721 cacacactgg tggcggctgc tgggaaaaac ttttttaccc accctcctgt tgctgattgt
    21781 ggtcttactg gtgttgttga aactggctgg ctttcttcct tccattgcag cagaccacct
    21841 gtacataggg agcatcatcc tcgccgtcac cagctgggct tggtggctgt atcaatacca
    21901 ggattggtac aacgacgttt acatcatcac ctctgagcag ctcgtggatg ttaaccgaaa
    21961 gccacttggc aaagaagagc ggcgttcggc gccggtgcag aatatccaga ctgtggagta
    22021 taaacgcaaa ggcatcattg gcctgatact gaacttcggc acggtacata ttcaaattgg
    22081 caatcaggaa ctgtctttcg atgacgtcta caatccggca gccgtccagg cggaaatctt
    22141 ctcacggttt aagcatcgag accaaaaaac actgcgcttg gaacaggaaa agcttgtgga
    22201 ttggatcaag gcctatgatg aaatgaaaaa ggaagataaa gatcctttcg atagccagta
    22261 ggttaacggt aaaattgctc aggaacaggt agaaaggatt ttacatggca ttacgagaaa
    22321 tcatcaccat ccctcatctc attttgcgca agaaagcccg caaggtgacc gattttggca
    22381 gggattttca ggtgctggta accgatatga tcgacaccat gagagaagct ccaggcgtag
    22441 gcttggcggc tccgcaggtg gcggaatctc agcgcgtcat tgtggttgag tacggcgatg
    22501 aagaagatga agaaaaaccc aagaagctct tcgtggtggc caatcccgaa attatcagtt
    22561 cttcagaaga taaggtcatc ggtatcgaag gctgcctttc tgttcctgag ctggtcggtg
    22621 aagttgaacg ctatcacgcc attattgtaa aaggtcagaa ccgctttgga aaaccaacaa
    22681 agatcaaagc cagcggctgg ctggcgagga tttttcagca tgaaatcgat cacctggacg
    22741 gagttttata ccctgaccgc gcgctgcggg tctggaaacc cgcgccggag gaagacatcc
    22801 cgcttgatta ggttatgcac cttacccata tttcgctgac gaatttccgc cttttttccc
    22861 gcctggatat gaacgtcccc cggcggattc tcttgttgtt gggcgccaat gcccagggga
    22921 agacctcgct ccttgaagcg atttattatc tggctacctt cacctcattt cacgcccaaa
    22981 acgaccgtca actgcttaac tttatcgcca cgcgagaaaa cctggccgtt gcgcgactgg
    23041 tggctcaata tacacgagcg gaacgcgcgc accggttgga agtgcgcctg atccaggatt
    23101 cgaacgggaa cggcggtgcg cggctgcgca aagaagtgct gttggatggc gtgaagactc
    23161 ctcccgctca ggtgattggg caatttaacg cggtcatctt tctgccgcaa atgaccggca
    23221 tcatcgaaga tggaccggag gagcgacgca ggtatttaaa cctgaccatc tcccaatcca
    23281 acccggttta tacccgtgcc cttgccgatt acaaccaggc gcttacccaa cgcaacgctc
    23341 tgctgaaact cctcaacgag cgcggcggcg atcgctcgca gttggattat tgggatgaaa
    23401 ttttggcggc gcgcgctgcg ctgctgatta ccacgcgcgc ggttgtcatt caagagctgg
    23461 aagaaatagc caagcgcgtg cacgaacgcc tgactggttc gaaagaagtg ctgcgcctga
    23521 attatcaacc ttcatacgac cctctccccg cgccgcaagg tcaattcgcg ttgcccatgc
    23581 aggtgcaggt tcaaaggagc ggtttgagcc aggaaaaagt gaaacagggg ttcatcgccc
    23641 gcctggccga attgcgcgcc gaggagatcg cccgcggtat caccacaatc gggccgcacc
    23701 gcgacgaaat gcgtgtgttg agtaacggca ttgacctcac cgattttggt tcacgtggtc
    23761 aaatacgcac cgcgctgctt tcactcaagc tggccgaggt ggattggtta aaagagaaat
    23821 ctggtcaatg gccggtgttg ctgttggatg aaaccctcgc ggaactgga
//
LOCUS       DAOSPM010000023        23254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2219_length_23254_cov_12.779689, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000023 DAOSPM010000000
VERSION     DAOSPM010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 23254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23254
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2219_length_23254_cov_12.779689"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="PLW25_03510"
     CDS             complement(<1..1039)
                     /locus_tag="PLW25_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53588.1"
                     /translation="MNWLKKGAQMNRSEKLRQKRQPAAQTRSAVPAAAASRLAADRVV
                     ARRASTSAENRHSVGTQPRRKVVYSVGANGVETRLPALPILRFSWQWVSGILTILLLM
                     FIIVLVNTPIFKVNSFEVHGLSTYSSADFNPLLNGRRDSIFLVNTNEIFDSILVAFPE
                     LVGSQVEIGLPNRIVITAAERKPVVLWHSSTGDYWIDAEGVILAPRGEGSGLLTIESA
                     SLPPLLVERHDPRNFTEYVTMVFERKERPSNPSERAAHIRPEVLEAALQMNSLLPQGA
                     TLLYDSVSGMGWRDPGGWEVYFGNDLSNIQMKLVMYQVILGRLQELGISPEMISVEHV
                     DAPYYRMEYALW"
     gene            complement(1036..2142)
                     /locus_tag="PLW25_03515"
     CDS             complement(1036..2142)
                     /locus_tag="PLW25_03515"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOS53589.1"
                     /translation="MTGKIRLAVIFGGRSGEHEVSLMSARSLLAVLDADRYAITQIGV
                     TPAGKWLHGDDVIGRFEKHDYTTLSPVALLPEPLNGALFAVKNGQMERLPDIDVVFPL
                     IHGTFGEDGTLQGLLELADLAYVGGGVLASAVGMDKSLCKDVLRAHQIPVVDSLLLNR
                     SSIRTDINASIEQIEADFAYPVFVKPVNLGSSVGITRCSNRSDLMKGLLEAARFDRRI
                     LVERAINARELEVSVLGNENPIASVPGEIQPQEDFYTYSAKYLTNQSRLIIPADLDED
                     TSRLVSDTAVSAFRAIDGAGLARVDFLLDRQTGEVFVSEINTIPGFTAISMYPKLWQA
                     SGISYTELINRLVELALERKHDRDITVRTYGRDG"
     gene            complement(2139..3059)
                     /gene="murB"
                     /locus_tag="PLW25_03520"
     CDS             complement(2139..3059)
                     /gene="murB"
                     /locus_tag="PLW25_03520"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOS53590.1"
                     /translation="MDEKVLLKRFAQRLKKDVPLANLTTAKVGGAARYFLNAVNAAQL
                     AEDVAFLWAEEIPFFILGSGSNILVSDAGVDCVVIHNKANHIHLVEKGDSSYVQAESG
                     AILITAARQAALRGYTGLEWATTIPGTVGGAVYGNAGAYGASMQQHLLLVDILHRGKG
                     CLSLNTEQMGYSYRSSALKREPGMAVILSARLAIKKGDKERIKELINEFSARRKQTQP
                     PGPCIGSTFKNPAGDSAGRIIDSLGLKGTRVGGVQVSPVHANFFVNEGSASARDYYDL
                     IHLVQRIVEEKTGIKLEPEFELIGDWKEQA"
     gene            complement(3081..3272)
                     /locus_tag="PLW25_03525"
     CDS             complement(3081..3272)
                     /locus_tag="PLW25_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53591.1"
                     /translation="MNPPRKNSYENVNHDFDTPRYFSEFPRIQVKRQINQPVADFRAL
                     SLQDRVELMEKLIERVREL"
     gene            complement(3272..4651)
                     /gene="murC"
                     /locus_tag="PLW25_03530"
     CDS             complement(3272..4651)
                     /gene="murC"
                     /locus_tag="PLW25_03530"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOS53592.1"
                     /translation="MMKRIHLIGIGGSGMSSIARVLLEQGLTVSGSDRTLSPLALNLR
                     AAGAIVYEGHDPQHIEGVDMVVRSSAIADDNPEVAAALALGIPVLKRQDFMRDLTRER
                     LCIAIAGSHGKTTTSTMMAWSLVQLGLDPGYILGGVSRNLANNAHAGSGELFVVEADE
                     YDYMFLGLNPAVILLTNVEYDHPDCFPTAEKYQAAFSQFVHQLEPGGLLVTNADDPGA
                     AAKRHDIPESARVVTYGLNGPADYSAIDWRVNAAGGYDFKISALQIHAPVAQVSLAVP
                     GMHNVRNALGVLSALHALGFPVQQAADALSFYSGSSRRFEVIGEVAGITIIDDYAHHP
                     SKISATLSAARVRYPRRRLVVVWQPHTYSRTQALESDFIRAFDDADLLVVTAIYAARE
                     QARAYSAEQMVEKMKAPSVSYIPQIPDVVDFLLKTLQKGDVLIVLSAGDADQVCREVR
                     TRLQERKGE"
     gene            complement(4648..5190)
                     /locus_tag="PLW25_03535"
     CDS             complement(4648..5190)
                     /locus_tag="PLW25_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOS53593.1"
                     /translation="MISLDVLLWVFIVLFALVGAMRGWAKELLVTFSIILAMFSLTVL
                     EAFLPFFKETIQQSQPSTVFWLKSGILMALVFFGYQTPKIPRLAESERFIRHVLQDTM
                     LGAVLGGINGYLIFGTVWYYLHSTGYPYPFVLPPDALTQSGQTALRWIENLPPAWLMT
                     TPTIYIAVAVCFVFVLVVFI"
     gene            complement(5192..6298)
                     /locus_tag="PLW25_03540"
     CDS             complement(5192..6298)
                     /locus_tag="PLW25_03540"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOS53594.1"
                     /translation="MRLLICAGGTGGGVYPALAVLQTVADRVEQTLWVGGQGGMEAAL
                     VARTGVPFAEISAAGVHGVGLAALPGNLIKLGRGYSQARRILRDFQPHVLFFTGGYVA
                     VPMALAGAGVRKLLYVPDIEPGLALKALAKFANIIALTDESSTRYFSKKKQVVVTGYP
                     LRGELQKVPREQALQTFKFTAELPVLLVIGGSKGAHSINQAVFDNLPTLLHKTQVLHL
                     TGQQDWEEANSIRLALEVHLGARYQPYPYLHEEMSAAFSCANLVLSRAGASTLGELPF
                     YGLPAILVPYPYAWRYQKVNANHLTQSGAALTVENNELSDRLGALVDELLSDPQRLTD
                     MGKKMGVMAAPDAARKIADLLVLLAEEQGKERRA"
     gene            complement(6222..7466)
                     /locus_tag="PLW25_03545"
     CDS             complement(6222..7466)
                     /locus_tag="PLW25_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOS53595.1"
                     /translation="MTTTGLNPRPRRTHFRRLSLRESFDLPLAVVVLTLFAVGLLMIY
                     SASWQYASVQGFSEYQMVMRQMLIGLAGLGGIVIITLIDYHQYQRFVVPGMLLIVSLC
                     LLVLLFGSETQFGARRGLFSGSIQPSEFAKLGVIIYLSFWLYNKRGRLHMWSYGLIPL
                     LAILGVTAGLILMQPDISAAATIVLLGGVLFFIAGGKMSQILVVVVATALLGFLLVKV
                     SSTASTRLSDYWNGLQDPKNASDHVKWSLEAIINGGLFGVGIGRSTTKFIGLPVATTD
                     SIFAVITEETGLLGAFAVIILYVLLLWRGLTIADRAPDTLGKYLASGITLWIFIEAII
                     NIGVLVNLLPFAGNALPFISSGGSSLITTLAAVGILMGISRQSKLAKLSAERSPRNAV
                     VDLRRRNRRGSVSRAGSAADGR"
     gene            complement(7463..8842)
                     /gene="murD"
                     /locus_tag="PLW25_03550"
     CDS             complement(7463..8842)
                     /gene="murD"
                     /locus_tag="PLW25_03550"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOS53596.1"
                     /translation="MNRFNGKRVLIIGAARQGLALARYLINQQALVTLNDQQTPEQLQ
                     QVMQPFAGSGVEWALGGHPLHLLDGKDLVCVSGGVPLTLPLVQAALERGIPLTNDSQI
                     FMETVKAPVVGITGSAGKTTTTSIFGRMAAAALKSPCKAWVGGNIGNPLIEFSEEIDA
                     DDLVIQEFSSFQLELMTTSPHIAAVLNITPNHLDRHADMDEYTRAKSRILFHQSTADI
                     AVLNREDMGAWNLRPLVRGALLSFGMERSPSVGNQVYLKDPNIHAEVEGKATELFPVS
                     LISLRGKHNLMNVLAACALALACDFPVEAMVRGIEGFNGVKHRLQFVREVNGVRWYND
                     SIATAPERSLAALQSFQEPIILLLGGRDKNLPWQPLAQEVKKRARHAVLFGEAGSKIA
                     AALGTPTEVDYLQSISQVGTLQEAVLQAARLASQGDVVLLSPGCTSYDAFKDFEERGE
                     AFIEWVNHL"
     gene            complement(8850..9842)
                     /gene="mraY"
                     /locus_tag="PLW25_03555"
     CDS             complement(8850..9842)
                     /gene="mraY"
                     /locus_tag="PLW25_03555"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOS53597.1"
                     /translation="MKETALALSLSGLGFILSVIWGGPLLRVLKHFKMGKIIRVEGPE
                     SHITKMGTPTMGGLVIVGTVVLFTVLLNAASILGLHLSGRSVLLPVVVMVSFSLLGAI
                     DDWEGIRGPRRGLGMRARTKFLLQVILALIAAFILKNVLDVPDMYWPGLKINIELGVW
                     YIPLATFIIVAMSNAVNFTDGLDGLAGLICATAFAAYGGIALMQGNIFVARFCFTVVG
                     ALFGFLWFNVHPAMMIMGDTGSLSIGAVLGVVALMTGQWVLLPIIAIIPVSEAASDVL
                     QILYFKATKGKRLFKMAPLHHHFELLGWSETQIVQRFWLIGLLAAMIGMALALG"
     gene            complement(9839..11257)
                     /gene="murF"
                     /locus_tag="PLW25_03560"
     CDS             complement(9839..11257)
                     /gene="murF"
                     /locus_tag="PLW25_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOS53598.1"
                     /translation="MLTLADVIEALTGVRPIGATPGISEGVVDSRLAIPASLFVAIKG
                     NRTDGHQYVSHAFANGARFALVQHEIDPSIPVVDLGKPISADHAFPPLPFALCVKDTT
                     KALQTVAAFWRRKFTTRVIGITGSVGKSTTKELTAEILSQRYRTLKNQGNFNNEVGLP
                     LTLLRLGPGYDRVVLEMGFYEPGEIASLCEIAKPQVGILTNIGTVHASRAGSQETIAH
                     GKSELVRALPPAPTGSAILNIDDPFIREMANLTQAKVIYYGLDSKADLWADEIESRGL
                     DGIHFKIHHGADAFNLQAPLIGRHSIHTILAAAAAALNEGLTWQEIILGLQHATSQLR
                     LVAVKTRQGALILDDTYNASPESMLAALNLLNELPGRKIAVLGGMNELGQYEQLGHNK
                     VGIRAAEVVDKLIAFQEKAQLIATAAQKAGLPGEFIEIIDDENEVIQALNLTLRPGDV
                     VLVKGAHSLRMDRIVQAMEVEG"
     gene            complement(11266..13038)
                     /locus_tag="PLW25_03565"
     CDS             complement(11266..13038)
                     /locus_tag="PLW25_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOS53599.1"
                     /translation="MRKRFMRYHFLGISLSVFAVVIAVQMVRIQSSTHADYLNDWAKD
                     YGYELRTIESERGYIYDRYGHLLAGNKEVYEVGIELQFVRNPSAIASTLANVLGLDYQ
                     EVFNAANTKYVPGQQVYVTLADFVESYKIKEINDLKTQFENNNPYGLDTALPSLRGLT
                     WTAHLRRIYPEGNLAANILGFFTYYDREGGAGYFGVEEKYNTLLAGIKRNVVIPLDPY
                     ELQEIPTAPAGASLVLTIDREMQRTVEGILDKAVKTNGAAGGTIIVMDPNNGEILAMA
                     SNPRLDPNAYWENTKVFPPGVPYNKAVNQVYEPGSVFKILTMAAALDAGVVTSDTPFT
                     DTGQIEVGGWMIYNWDRGAWGNQNMIGCMQHSLNVCLSWIATQLGPTRFYDYLERFGI
                     GHRTNIDLAGEEVYPLSVPGDPMWYTVNLATNSFGQGVAVTPVQLLMAASAIANEGQM
                     VAPHVLKSYIQDGQQFDTAPQVVGRPITAETAKTLTDMLAISLEEEASTALVDGYRVA
                     GKTGTAEIAVNGQYSSNKTNASFVGWGPVDDPQFIVFVWLEKPSTSIWGSIVAAPVFS
                     NVVQEIVVLMNLPPDEIRMSLKEE"
     gene            complement(13038..13505)
                     /locus_tag="PLW25_03570"
     CDS             complement(13038..13505)
                     /locus_tag="PLW25_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53600.1"
                     /translation="MRLIQALKQLPWKIHARTVGFFLLSLVVVLVVASVYLSISGLAA
                     NAGLDAYRLDLKRQDLEREIAHRKATLALITAAPAMEERALSMGFKKIAPEDALYVIV
                     PGYSGRRTMVAAPPPGINETSRQLIKSVYRESLWDWLFTGVNSLGTGFLGDGS"
     gene            complement(13571..14515)
                     /gene="rsmH"
                     /locus_tag="PLW25_03575"
     CDS             complement(13571..14515)
                     /gene="rsmH"
                     /locus_tag="PLW25_03575"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOS53601.1"
                     /translation="MKNAHDSAAKPPHVSVLYHQIITALRPVSPGLYVDATIGAAGHA
                     WGILEKSSPDGQLLGLDTDPQALALSHQRLSIFHQRFHLLHSSYTELRNALLTLGWDH
                     VDGIVADLGVSSMQIDSPERGFSFLNDGPLDMRFDPSQPVSAETLVNHSTESELADLI
                     WRYGEERRSRHIARVIVQNRPFTSTLQLAETIKKASYGIRSEIHPATRTFQALRIAVN
                     HELDSLQIFLPQAVESLAPGGRLAVISFHSLEDRIVKQFFRRESRDCICPADQPVCIC
                     GHKASLIEVTRHPITPDGIEVSSNVRARSAKLRVAEKR"
     gene            complement(14515..14958)
                     /locus_tag="PLW25_03580"
     CDS             complement(14515..14958)
                     /locus_tag="PLW25_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOS53602.1"
                     /translation="MFKGFTGCYLHNIDEKGRLTFPVSFRDLLGENPFILKGFDQNLL
                     VMNANRFELLYERINNMNMANAQTRQLRRYIFSTAAQIEFDKAGRFIIPQPLRRIAGL
                     DGTALIVGIGKDIEIWNPDTYKIQEDQGEGPSSAAELVSSFDLTI"
     gene            15149..16096
                     /locus_tag="PLW25_03585"
     CDS             15149..16096
                     /locus_tag="PLW25_03585"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HOS53603.1"
                     /translation="MRSFFARFSILVVVLLGFTMLNGCITINLGGATPDSGQTLEPAP
                     TTPPPAAVQTPRQVLNEERLLNADYLSPLFQVPITLVGGVFSGVVDGSELKVTLQPGI
                     QYGDLNDDGVEDAAFILAESTGGTGVFSSLVVVFSEAGRFVQAPGVMIDDRPVIESLT
                     IENGVVKLQALVHSFNDTMVNPSTRIRAEYSLLDGKLVQTRLSSAFSGSEEHVITIDS
                     PRDGDTISGSVRLIGSMPIGPFENTLLLRITDLSGGELLHEGFMVQTEDLGAPATFDN
                     TVLIPGIAPGTRILLTLSELSMADGSPIALDSVLLVVGE"
     gene            complement(16208..16747)
                     /locus_tag="PLW25_03590"
     CDS             complement(16208..16747)
                     /locus_tag="PLW25_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53604.1"
                     /translation="MRFLIFSISIMLLLVSCNPTLSDTPDFISPLSTATARPVINDEN
                     PSPPAPTIAKPDQPVDSLTPLKTPNSFEPGPGDELKQRSAAFVDNAQLMILESDPIQV
                     NLQIKGSLPTPCHILRVAISPPDKENHIAISVYSLVDPAVVCIQVLQEFETSVPLGAY
                     PYGHYLVYVNDMLVGEFDG"
     gene            complement(16841..17224)
                     /locus_tag="PLW25_03595"
     CDS             complement(16841..17224)
                     /locus_tag="PLW25_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOS53605.1"
                     /translation="MRKVKLAVNGTLMRGLELNHNLLSVGAVFLCQTVTAPTYRLWSI
                     GDLYPAMLRDDHLGAAIHVEIWSLSASALVAVLQQEPPGLCLGRVELQGGELVFGVLA
                     EPYLISGQQEITRWGGWREYRQSQP"
     gene            complement(17235..18758)
                     /locus_tag="PLW25_03600"
     CDS             complement(17235..18758)
                     /locus_tag="PLW25_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOS53606.1"
                     /translation="MLAKVNACAVIGLDGVVVDVEVDLTPGIKPPIIIVGLPDVSVQE
                     SRERVQSAIRNTGLEYPRKRVRVNLAPATVRKEGSAYDLPIALGILIANRQLPPQCLQ
                     DALVMGELSLDGSVRHIRGVLPMAAVARAQGYKRVYVPAEDAPEAAIFPDLEVIPVSS
                     LADLFQHLVGQVIIDPQPALAVEQVSALVQTDFREIKGQEHVKRALEVAAAGAHNLLM
                     IGPPGAGKTLMARALPSILPSMTLEEALDVTRIYSVADALPEGIPLLKSRPFRAPHHT
                     ISHAGLVGGGNWPHPGEISLAHRGVLFLDEFPEFGSRTLEVLRQPLEDKIITISRAQG
                     TLTFPANFQLVAAMNPCPCGYYGDPTRACTCAPGIVTKYQRRISGPLMDRIDIHVEVP
                     RVDYEKLSDARLGEPSAVIRTRVESARQIQRERFADTSLTSNADMHPAQVRQYCSLDE
                     SCRGLMRTAMNQLQLSARAYHRILKLARTIADLAGSPHISTIHLAEALQYRPRVSDV"
     gene            complement(19093..20022)
                     /locus_tag="PLW25_03605"
     CDS             complement(19093..20022)
                     /locus_tag="PLW25_03605"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HOS53607.1"
                     /translation="MPERRFPFAHTFSIVARDPKNGELGVAVQSHWFSTGSIVAWAEP
                     GVGAIATQSMAEVSYGPLGLSAMREGKPASVVLANLLAKDANREVRQVAMVDGKGTVA
                     VHTGQRCIAEAGHQTGYQYSVQANMMLRNTVWPAMAAAYEAAKGSLADRLLAALDAAQ
                     AQGGDIRGQQSAALLVVSGESHNEPWKGVLVDLRVEDHSEPLVELRRLLTVQHAYEHM
                     NRGDELLSQGLIDQAFSAYEQAAALAPHLHELPFWQAVTLLDSGREQEALPILKRVFQ
                     ANPNWLLLLERLPAAGLLKDDRRMLRRVRKLLG"
     gene            complement(20051..20959)
                     /locus_tag="PLW25_03610"
     CDS             complement(20051..20959)
                     /locus_tag="PLW25_03610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS53608.1"
                     /translation="MTIPDLSLLAATSWQDYELLDSGAGMKLERFGAYRLIRPEAEAV
                     WQRALPENEWRAADALYKPAPEENGGHWEFRKKMPDQWKLSYGQLAFWVKTSSSRHLG
                     VFPEQSCQWDWAEKVIHLSGGKPRVLVLFGYTGIASLSAAAAGAQVTHLDASRKVITW
                     ARENQTLSRLDSLPIRWILDDALKFVQREQRRGSCYDALILDPPKFGRGPKGEIWDFY
                     KLLPDLLAACRAILSPDPLFVQLTAYAVKASAATMYNAILEMTARFKGITQAGEIGLL
                     ERSAKRFLSTAIFARWCRADIHQEMG"
     gene            21067..>21251
                     /locus_tag="PLW25_03615"
     CDS             21067..>21251
                     /locus_tag="PLW25_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative C-S lyase"
                     /protein_id="HOS53609.1"
                     /translation="MFQAPERRNTDSVKWGLFTEDVLPLWVADMDFTSPPVVIEALQQ
                     RVSHGVFGYALESQELK"
     assembly_gap    21252..21351
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21352..21915
                     /locus_tag="PLW25_03620"
     CDS             <21352..21915
                     /locus_tag="PLW25_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative C-S lyase"
                     /protein_id="HOS53610.1"
                     /translation="DEIHCDLVYKGYQHIPIASLDDEIAASTVTLIAPSKTFNIAGLD
                     CSILICQNEELRKRILHSKRGLMGGVNLLGMTAAAAAYREGASWLSEVLAFLESNREH
                     LMAYIEHNLPRIHMVKPEATYLAWLDCRELELAPSPREYFFQRARVALNDGRDFGEPG
                     TGFVRLNFGCSRETLDNALERMCHSLS"
     gene            complement(21969..22181)
                     /locus_tag="PLW25_03625"
     CDS             complement(21969..22181)
                     /locus_tag="PLW25_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOS53611.1"
                     /translation="MAERFVGTVKWFNPAKGYGFIGRENAEDVFVHFSAIEMEGYKVL
                     KEGQQVEFSIEKGPKGLQAAEVKPVE"
     gene            22332..>23254
                     /locus_tag="PLW25_03630"
     CDS             22332..>23254
                     /locus_tag="PLW25_03630"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS53612.1"
                     /translation="MRNERKLWLLFFLLLPLGFMAFPKAGAAPENLTLSLQNDRAALV
                     NGDAQMELTAIQQALEFQTWRGDLWQRAGRLYMDQGDYQNALHSFEKAQELGQLEPLG
                     QVWLSDMLIANGYSDAAKYVLQSIHTDDVFILLQAAALMAPLNDHEGLRQLLQKAYAS
                     EPTNSEVNYQMGIYLMTEDPGRAVAHFELAKRDQSRKAAAAYLVENIQQYGALEDHGE
                     WYLYAGQALARVNEWNAAFQAFSKAVQSLPDNATALALLGESQFQLGLDGGQNLEKAW
                     ELDPEGEITNGVLGIYYQRQGNLEKALEHLR"
BASE COUNT         5439 a         6092 c         6460 g         5163 t
ORIGIN      
        1 tccatagtgc atactccatc cggtaatatg gcgcgtcaac gtgttccacg ctgatcatct
       61 cgggagaaat acccagctcc tgaaggcgcc caagaatcac ctgatacatc accagtttca
      121 tctggatatt gctcaggtcg ttgccgaaat aaacttccca accaccggga tccctccagc
      181 ccatacctga aactgaatca taaagaagcg tcgctccttg aggaaggaga gaattcattt
      241 gcagcgccgc ttcaagtact tccgggcgaa tatgggcagc gcgctcggaa gggttcgatg
      301 gtctttcctt gcgttcaaaa accatggtga cgtactcggt gaagtttcgc gggtcatgcc
      361 gctcaacgag cagcggaggt aaggacgcgc tctcaatcgt cagcaaaccg ctgccctctc
      421 cacgcggagc gaggatgaca ccctcggcgt caatccagta atctccggtg ctggaatgcc
      481 ataaaacgac cggttttcgt tcggcggcgg tgatgacaat gcggttgggc aatccgatct
      541 caacctggct gccgaccagt tcaggaaaag cgaccagaat ggaatcaaat atctcattgg
      601 tgttgacgag gaagatcgaa tcacgcctgc cgttgagcag cggattgaaa tcagcgctgg
      661 agtaggtact caaaccatgt acctcaaagc tattgacctt gaagatgggt gtgttgacta
      721 agacgatgat gaacatcaat aacaggatgg tgaggatgcc agacacccat tgccagctga
      781 atcgcagaat gggcagcgcg ggcaggcggg tttcgacgcc attggcgccg acgctgtaca
      841 ccaccttgcg gcggggctgc gtcccgactg aatggcggtt ttccgctgag gtgctggcgc
      901 ggcgagcgac aacccgatct gctgccagcc gggaagcggc tgcagccggc accgcagaac
      961 gcgtttgcgc tgccggctgg cgcttctgcc gcagcttttc agaacggttc atctgggcgc
     1021 ctttctttaa ccagttcatc catctctccc gtaggtacgc acggtgatat cgcggtcgtg
     1081 ttttctctca agcgccaatt cgaccaggcg attgataagt tcggtatatg aaatgccact
     1141 tgcctgccaa agttttggat acatgctgat ggcggtgaaa ccaggaatgg tattgatttc
     1201 gctgacgaaa acctctccag tctggcggtc gagcaagaag tccacgcgcg ccagcccagc
     1261 gccatcaatc gcgcgaaagg cgctgaccgc ggtgtcgctg accagcctac tggtgtcctc
     1321 gtccaggtca gccgggatta tcaggcggga ctgattggtg aggtatttgg ctgaatatgt
     1381 ataaaaatct tcctgcggtt ggatttctcc gggaacggag gcgatggggt tctcgttgcc
     1441 cagcacgctc acttcgagtt cacgggcgtt aatggcacgc tccaccagaa tccggcggtc
     1501 aaaacgagcc gcctccaata atcctttcat taaatcagag cgattggagc agcgggtaat
     1561 tcccaccgag gagcccaaat tgaccggttt cacaaagaca gggtaagcaa agtcggcttc
     1621 gatttgttct atgctggcgt tgatatccgt gcgaatggaa gagcggttga ggagcaacga
     1681 atcgaccacg ggaatttgat gagcgcgcag cacatctttg caaaggcttt tatccatacc
     1741 gaccgcagac gccagcacgc cgccgccaac ataagccagg tcagccagtt ccagtaagcc
     1801 ttgcagcgtg ccgtcttcac cgaaagtacc atgaatgagc gggaagacta catcgatatc
     1861 aggcaggcgc tccatctgcc cgttttttac cgcgaacaac gcgccattga gcggctcagg
     1921 cagaagggcg accggtgaga gcgtggtgta atcgtgtttt tcgaagcggc caatcacatc
     1981 atcaccatgt agccattttc cagcaggagt gacgccaatt tgggtaattg cgtaacggtc
     2041 agcatcaagg acggcaagaa gcgagcgggc gctcatcagg gagacttcat gctcacctga
     2101 tctaccgccg aagatcactg ccaagcgaat tttcccagtc atgcctgttc cttccagtca
     2161 ccaatcagtt caaattcggg ttccagtttg ataccggttt tttcttccac gattcgctgc
     2221 acgagatgaa tcagatcgta gtaatcacgg gcgctggcgc tgccttcgtt gacgaaaaag
     2281 ttcgcgtgca cggggcttac ctgcacgcca cccacacggg tgcctttgag gccgagcgaa
     2341 tcaatgatgc gcccggcgga atcacccgcg gggttcttga aagtagagcc tatgcatggg
     2401 ccgggaggct gggtttgctt cctcctggct gaaaattcgt ttataagttc ttttatcctt
     2461 tctttgtcgc cttttttgat ggcaagccgg gcggagagga taacagccat gcccggttcg
     2521 cgtttgagcg cgctgctacg ataggaataa cccatttgct cagtgttgag cgacaggcag
     2581 cccttaccac ggtgcaagat atctaccagg agaagatgtt gctgcattga agcgccatac
     2641 gccccggcgt tgccgtaaac cgctccgccg accgtgccgg ggatagtggt tgcccactcc
     2701 agcccggtgt acccgcgtaa cgccgcctga cgggcagcgg tgatgaggat ggcgccggat
     2761 tcagcctgta catacgaaga atcgcctttt tcaaccagat gaatgtggtt tgctttattg
     2821 tggatgacca cgcaatcgac cccggcatca cttaccagga tgttggaacc gcttcccaga
     2881 atgaagaagg ggatttcttc agcccacagg aaggccacat cttccgcgag ttgagctgca
     2941 tttaccgcgt tcaggaaata gcgcgccgca ccacccactt tggcggtggt caggttcgcc
     3001 agaggaacat ccttcttcag gcgttgggca aaacgtttga gaagtacctt ctcatccatg
     3061 ggcgcggtca tgctcctgtc ttagagttct ctcactcttt caatcagctt ctccatcaac
     3121 tccacgcggt cttgcaggct caaagcgcga aaatcagcca caggttggtt gatctggcgt
     3181 ttcacctgaa tacgggggaa ctcagagaag tagcggggcg tatcaaaatc gtgattgaca
     3241 ttctcataac tattttttct gggcgggttc attattcacc tttcctttcc tgcagacggg
     3301 ttctcacttc acgacaaacc tggtcagcat caccggctga cagaacaatc aacacatctc
     3361 ccttttgcag cgtctttaat aaaaaatcca ccacatcggg aatttgaggg atataactca
     3421 cagagggagc tttcattttt tcaaccattt gctcggcgct gtacgcacgc gcctgttcgc
     3481 gcgctgcata gatggcggtg accaccagca ggtcagcatc atcaaaggcg cgaataaaat
     3541 cactttccag cgcctgcgtg cgagagtagg tgtgcggttg ccaaacgaca acaaggcgac
     3601 ggcgggggta gcgtacacgc gcagcgctga gtgtggcgct tatcttgctg gggtgatgcg
     3661 catagtcatc aataattgtg atgcctgcta cttcaccgat aacctcgaag cgccggctgg
     3721 agccgctata aaaggagagg gcgtccgcgg cctgctgcac tgggaaaccg agggcgtgaa
     3781 gtgcagaaag cacaccgaga gcgttgcgaa cgttgtgcat tccgggaaca gccaaactga
     3841 cctgggctac gggggcgtga atctgaagcg cactgatttt aaaatcatac ccaccggcgg
     3901 cgttgacgcg ccaatcaatg gcggaataat cggcgggccc gttcagcccg taggtcacta
     3961 cgcgggcaga ttcgggaatg tcgtgacgtt tcgcggctgc accagggtca tcggcgttgg
     4021 tcacgagcag tccacccggt tcaagctggt gaacaaactg cgaaaacgcc gcctggtatt
     4081 tttctgctgt cgggaagcaa tcgggatggt catattcgac gttggtaagg aggatgaccg
     4141 ccgggttgag accgaggaac atatagtcgt attcatccgc ttcaacgacg aataactccc
     4201 cgctgcccgc gtgcgcgtta tttgcgaggt tcctggagac gcccccgagg atgtacccgg
     4261 gatccagccc gagttgtacc aggctccagg ccatcattgt ggaggtggtt gtttttccgt
     4321 gagaaccggc aatcgcaata cacaggcgct cccgggtcag gtctcgcatg aaatcctggc
     4381 gcttgagcac agggataccc aatgcgaggg ctgcggcaac ttcagggtta tcatcagcga
     4441 tggcggaaga acgcaccacc atatccacac cctcgatatg ttgtggatcg tgcccctcat
     4501 atacgatcgc gccggcagcg cgcaggttaa gcgcaagcgg agaaagggtg cggtcagagc
     4561 cgctcacagt aagaccctgc tccaacaaaa cacgcgcaat ggaggacatg ccgctaccgc
     4621 caataccgat caggtgtatg cgcttcatca tatgaacacc accagtacga agacaaagca
     4681 aaccgcgacc gcgatataaa tggttggagt ggtcatcagc caggcgggcg gaaggttttc
     4741 gatccagcgc agggcagttt gtccagattg cgtgagcgcg tcgggcggca gcacaaatgg
     4801 gtaaggataa ccagtggaat gcaggtagta ccaaactgtt ccaaaaatca ggtatccatt
     4861 tataccgcca agaacagcac ccagcatggt atcttgaagc acatgacgga taaagcgctc
     4921 gctttcagcc aacctgggaa tcttgggcgt ctggtagcca aagaagacca gcgccatcag
     4981 gatgccgctt ttgagccaga agacggttga tggttgggat tgttgaatgg tctctttaaa
     5041 aaagggcagg aatgcttcaa gcacggtaag cgaaaacatg gccaggatga tgctaaaagt
     5101 taccagcagc tctttagccc agccgcgcat ggcgccaaca agcgcgaaaa gaacgataaa
     5161 cacccagagc agtacgtcca gactgatcat atcaggcgcg cctttctttg ccctgttcct
     5221 ctgcgagcaa aaccaggagg tcagcaattt tccgtgctgc atccggggca gccatcacgc
     5281 ccatcttttt gcccatatcg gtaaggcgct gtgggtcaga aagcaattca tccaccagcg
     5341 cgcccaacct gtctgaaagc tcattatttt caacggtcag cgcggcaccg gactgtgtga
     5401 ggtggttggc gttcaccttt tgatagcgcc aggcgtaagg gtagggtacc aggatggcgg
     5461 gaagcccata aaaaggcagc tcaccgagtg tggaggctcc agcccgtgaa agcacaaggt
     5521 tggcgcaaga aaaggcagcg ctcatttctt cgtgtagata tgggtaaggt tgataacgcg
     5581 ctccaagatg cacttcaagc gcgagcctta tggagtttgc ttcttcccaa tcctgctgac
     5641 ccgtgagatg cagcacctgg gttttgtgta acaaggtggg gaggttatca aacacagcct
     5701 ggttgatgga atgggcgcct ttgcttccgc cgatgaccag cagcactggt agttctgcgg
     5761 tgaatttaaa tgtttgcaac gcctgctctc gcggtacctt ttgcagctct ccacgtaatg
     5821 ggtagcctgt aacgactacc tgttttttct tgctgaaata cctggtagaa ctttcatcgg
     5881 tcagggcgat gatattggca aacttggcca aggctttaag cgccaaaccg ggttcaatat
     5941 caggaacata gagcagttta cgcacaccag ctccagccag cgccataggc accgccacgt
     6001 aaccacccgt gaagaacaac acgtgcggtt gaaaatcgcg cagaatgcgc ctggcttgag
     6061 aataaccgcg cccgagtttg atcaggttgc ctggcaaggc agccaaccca acgccgtgaa
     6121 caccagcggc gctgatttca gcaaacggaa caccggtgcg cgcgactaga gcggcttcca
     6181 taccgccctg accgcccacc cacagcgtct gctcaacacg atcagcgacc gtctgcagca
     6241 ctgccagcgc gggatacact cccccgccgg ttccgccggc gcaaatcaac aaccgcattc
     6301 ctcgggctcc tttctgcgct taatttcgca agcttagatt gacgtgaaat tcccattaaa
     6361 atacccactg ctgccaaagt agtgatcagg ctggatccgc ctgaactgat aaacggtagc
     6421 gcgttgccgg caaaaggcaa caggtttacc aaaacaccta tgttgatgat ggcttcaata
     6481 aagatccaca gggtaatacc cgatgccaga tattttccaa gcgtatcagg tgcgcggtca
     6541 gcgatggtga gaccacgcca cagaaggagc acatataaaa taatgaccgc aaaggctccg
     6601 agcagcccgg tttcttcggt gatgaccgcg aaaatggaat cagtggtagc aacgggcagg
     6661 ccgatgaact tggtagttga ccgaccgata cctaccccga acaagccgcc gttgataatt
     6721 gcttcgagag accatttcac atgatctgat gcgttcttgg ggtcttgcag accattccag
     6781 taatccgaaa gacgggtgga ggcggttgaa cttaccttta ctaataagaa ccccaataac
     6841 gctgtggcga caaccactac cagaatttgg ctcatcttac cgcctgcgat aaagaaaagc
     6901 accccaccca gcaggacaat ggtggcggcg gcggaaatat ctggctgcat caaaatcaag
     6961 cctgcggtaa cacctaggat ggcaagcagc gggattaacc catagctcca catgtggagc
     7021 cgaccccgtt tgttgtacaa ccagaaactg aggtagataa tgacgcccaa tttggcaaat
     7081 tcagaaggct gaatggaacc ggagaagaga ccgcggcggg cgccaaactg agtttcagaa
     7141 ccaaaaagca acaccagaag gcataagctg acaatcagca gcattccggg cacaacaaac
     7201 ctctggtact ggtggtaatc gatcaaagtg ataatgacga taccgcccag cccggctaac
     7261 ccgatgagca tttggcgcat caccatttgg tattctgaga acccctgcac gctggcatat
     7321 tgccaactgg cggaatagat catcagcaga cccaccgcaa aaagggtgag aacgaccact
     7381 gccaagggta aatcaaaact ttcacgcaga ctcagtcggc ggaagtgggt gcggcgtggt
     7441 cggggattca atcctgttgt cgtcataagt ggttcaccca ttcaataaaa gcctcaccgc
     7501 gttcttcaaa atccttgaat gcatcgtagc ttgtgcagcc cggggagaga agcaccacat
     7561 cgccttgtga agccaggcgc gcagcttgta agacggcttc ctgcagtgta cctacctgac
     7621 tgatgctctg caggtaatcc acttcggtcg gggtccccag cgcagcggcg atttttgaac
     7681 cggcttctcc aaacagcacc gcatggcgtg cccgtttctt tacttcttgg gcgagcggct
     7741 gccagggcag gtttttgtct ctgccgccga gaaggagaat aatgggttcc tggaaagact
     7801 gaagcgccgc gagtgacctt tcaggggcag tggcgataga gtcgttgtac cagcgcacgc
     7861 cgttcacttc gcgaacgaac tgcagacggt gctttacccc gttaaaccct tcaattcccc
     7921 tgaccattgc ctctaccggg aagtcgcagg cgagcgcgag agcgcaggcg gcgaggacgt
     7981 tcatcaggtt atgcttcccg cgtagcgaaa tcaggctgac cgggaatagt tcagtggctt
     8041 ttccttccac ttcggcgtga atgttggggt ctttaaggta aacctggtta cccacgcttg
     8101 gagacctctc catgccgaaa ctcagcagcg cgccgcgaac caatggacga aggttccacg
     8161 cccccatatc ttcacggttc agtacagcga tgtcagcggt agactgatgg aagagaatgc
     8221 gggatttggc acgggtgtat tcatccatat ccgcatgtcg gtcaagatgg ttgggggtaa
     8281 tattcagcac agcagcgata tgcgggctgg tggtcatcaa ttccagctgg aaactggaga
     8341 actcctgaat caccaggtca tcggcatcga tttcttctga aaactcaatg agcggattcc
     8401 cgatgttgcc gccgacccag gctttacacg gggatttcag cgcagcggca gccatgcgcc
     8461 caaaaattga agtggtggtg gttttcccag ccgaaccggt aattcccacc actggggctt
     8521 tgactgtttc catgaaaatt tgcgaatcat tcgtgagcgg gatgccgcgc tcgagcgccg
     8581 cctgtaccag cggcagtgtt aatggaacac cgcctgaaac acaaaccagg tcttttccat
     8641 cgagcagatg aagaggatgt ccgccgagcg cccattcaac gccgctgcca gcaaacggtt
     8701 gcatcacttg ctggagctgc tcaggggttt gttggtcgtt gagggtgaca agcgcctgct
     8761 ggttgatcaa atatcgggca agcgccagac cctgcctggc ggcgccgatg atcagcacgc
     8821 gtttcccatt aaagcggttc atcgtctcct tatcccagtg ccagcgccat tccgatcatg
     8881 gcggccagca agccaatcag ccaaaaacgt tgcacaattt gcgtttcgct ccagcccagg
     8941 agttcaaagt ggtggtgcag tggagccatc ttgaacaggc gtttgccctt ggttgccttg
     9001 aagtacaaaa tctgcagcac atcgctggcg gcttcactca ccggaatgat ggcgatgatt
     9061 ggcagcagga cccactgacc ggtcatcagg gctaccactc ccaataccgc gccaattgac
     9121 agagagcctg tgtctcccat aatcatcatc gccgggtgta cattgaacca aagaaaacca
     9181 aaaagcgcgc caacaacggt gaagcagaag cgggcgacaa atatgttgcc ctgcatcagc
     9241 gctataccac cgtaggcagc gaaggcagtc gcgcaaatca acccggccag accgtccaga
     9301 ccgtctgtga agttcactgc gttagacatg gcgacgatga taaaggtcgc cagggggata
     9361 taccatactc ccagctcgat attaattttt aatcccggcc agtacatatc aggtacgtca
     9421 aggacattct tgaggatgaa agccgcgatg agggcgagga tcacctgtag taaaaacttg
     9481 gtgcgcgcgc gcatgcccag cccgcgccgg gggccgcgaa ttccttccca atcatcgatg
     9541 gcgccgagga ggctgaatga aaccatcacc accacgggca gcaagacaga acgaccgctg
     9601 aggtgcagac ccagtatcga agccgcattc aataaaacgg taaaaagcac cactgttccc
     9661 acgatgacaa gcccgcccat ggtgggtgtg cccattttcg taatgtggct ttcgggccct
     9721 tcgacgcgga tgatcttacc catcttgaag tgcttcagca cgcgcaataa tggaccgccc
     9781 cagataactg acagaataaa tcccaatccg ctcagggata aggctaaggc ggtctctttc
     9841 atccttccac ctccatcgct tgcacaatgc ggtccatacg caggctgtga gctcctttga
     9901 ccagaaccac atcacccggg cgcagggtca aattcagagc ttggatgacc tcgttttcat
     9961 catcaataat ttcgataaac tctccaggga ggcctgcctt ttgtgcggcg gtggcgatca
    10021 gctgcgcttt ttcctgaaag gcgatcaact tatccaccac ctcagcggcg cggatgccaa
    10081 ctttgttgtg accaagttgt tcgtattgac ccagttcgtt catccctccc aacacagcga
    10141 tcttcctgcc gggaagttcg ttcagcaggt ttaacgcggc cagcatagac tctggtgaag
    10201 cgttgtacgt gtcatctaat atcagggcac cctggcgagt tttcacagct acaaggcgca
    10261 actggctggt ggcatgttgc aggcctaaaa tgatctcctg ccaggtcaac ccttcgttaa
    10321 gcgcagcagc agctgcagcc aggatggtat ggatggaatg ccgaccgatt aacggcgcct
    10381 gaaggttaaa cgcgtccgcg ccgtggtgga tcttgaagtg aataccatca agcccacgag
    10441 actctatctc atccgcccac aagtctgcct ttgagtccaa cccataataa ataacttttg
    10501 cctgggtcag gttcgccatc tctctgatga aggggtcatc gatattgagg atggcgctgc
    10561 ccgtaggggc gggcggcagg gcgcgtacaa gttcgctttt tccatgggcg atggtttcct
    10621 gcgaaccggc gcgagatgcg tgtacagtgc caatattcgt aaggatgccg acctgcggtt
    10681 tggcaatttc acacaggctg gcaatctcgc cgggttcata gaaccccatc tccaatacca
    10741 cacggtcata gcccggcccg aggcgcagca gcgtgagagg aagaccaact tcgttattaa
    10801 aattgccctg gtttttaagg gtgcggtagc gctgcgagag aatttcagcg gttagttctt
    10861 tggtggtgga ttttccgaca ctgccggtaa tgccaatgac gcgggtggtg aacttgcgcc
    10921 gccagaatgc cgccacggtt tgcagcgctt tggtggtgtc cttaacgcac aaagcaaatg
    10981 gcagcggcgg aaacgcatgg tcagctgaaa taggctttcc aaggtcaaca acagggatgg
    11041 aagggtcgat ctcatgctgc accagggcaa agcgcgcgcc attggcaaag gcgtgcgata
    11101 catattgatg cccatcagta cggtttcctt taatggcgac aaacagggag gcaggaatgg
    11161 caagccgcga atctacaact ccttcgctga tacctggagt tgctccaatg ggccttacgc
    11221 ctgtcaacgc ttcaatcaca tccgccagtg tcagcataat ttccttcact cttccttcag
    11281 gctcatcctg atttcatcag gcggtaaatt catcagcacg acaatctcct gcacgacatt
    11341 gctgaatacc ggtgctgcga cgatcgaacc ccaaatggag gtgctgggtt tttccaacca
    11401 gacgaaaacg ataaattgcg gatcatccac cggtccccaa ccgacgaagg aggcgttggt
    11461 tttattgctg gaatattgcc cattgacagc aatttctgcg gtccctgtct ttccggcaac
    11521 gcgatatcca tccaccagtg cggtggaggc ttcttcttca agggagatag ccagcatatc
    11581 ggtgagggtt tttgctgttt ctgccgtgat gggtcgacct acgacctggg gcgcggtatc
    11641 aaattgctgt ccatcctgga tataagattt gagcacatga ggtgccacca tttgcccttc
    11701 gttggcgatt gcggacgccg ccatgagcaa ctgcactggg gtaacggcaa cgccctgacc
    11761 gaatgagtta gtcgcaaggt tgactgtgta ccacattggg tcaccgggaa cactgagggg
    11821 atagacttct tcccctgcaa ggtcgatgtt ggtgcgatga ccaatcccga aacgttcgag
    11881 ataatcataa aacctggttg ggccaagctg cgtggcgatc caggaaaggc atacgttgag
    11941 cgagtgctgc atgcagccga tcatattctg atttccccag gcaccgcggt cccagttgta
    12001 gatcatccag ccgcccactt cgatctgacc cgtatcggta aagggggtgt cagatgtgac
    12061 cacaccagca tcgagggcgg ctgccatggt caaaatttta aagactgaac ctggttcgta
    12121 gacctggttg acagctttgt tgtagggtac ccctggcggg aaaaccttgg tgttttccca
    12181 gtaggcgttg gggtcaagac ggggattgct ggccatggcc aatatctcgc cgttattcgg
    12241 gtccatcacg atgatcgtcc cgccggctgc cccattggtt tttaccgcct tatccaaaat
    12301 tccttcgacc gtgcgctgca tctcacggtc tattgtgagc accaggctgg cgccagcagg
    12361 agcggtaggt atctcttgca actcgtaggg gtccagggga atcacaacgt tgcgctttat
    12421 gcctgccagc agcgtgttgt atttctcttc aaccccaaaa taaccagccc cgccttctcg
    12481 gtcataatag gtgaaaaacc cgagtatgtt ggctgcgagg ttgccttcag ggtagatgcg
    12541 gcgaaggtgc gctgtccagg ttaacccgcg cagcgaaggc aatgcggtat ccaaaccata
    12601 agggttgtta ttttcaaatt gtgtttttaa gtcgttaatc tctttgatct tgtaggattc
    12661 gacaaaatcc gcgagggtga cataaacctg ttgaccagga acatacttcg tattcgccgc
    12721 gttgaaaact tcttgataat ccaatcccaa tacgttggcg agcgtcgaag cgatagccga
    12781 ggggtttcta acgaactgca actcgatacc aacttcataa acctctttat tgcctgccag
    12841 caggtgaccg taacggtcat aaatgtaacc gcgttcggat tcgatggtgc gaagttcgta
    12901 accgtagtct tttgcccaat cattcaggta gtcggcgtga gtgctggatt ggatacgaac
    12961 catctgcacc gcaatgacaa ctgcaaacac ggagagggag atcccgagaa agtggtagcg
    13021 catgaacctt tttctcatca gcttccgtct cccaagaaac cagtacccag agaattgaca
    13081 ccagtgaaga gccagtccca aagagattcg cgatacacgg atttaattaa ttgccgggac
    13141 gtttcattga tgcctggagg aggggcggcg accatcgtcc tgcgtcctga gtatcctggc
    13201 acgatgacat agagagcatc ttcaggggca atctttttaa aacccatgct cagcgctctc
    13261 tcttccatcg cgggtgcggc ggtgatgagc gccagggtcg ctttacgatg cgcgatttcg
    13321 cgttcaaggt cctgccgctt gagatcaagt cggtaggcat cgagcccggc gttggcagcc
    13381 aggccgctga tgctgagata gacactggca actacgagaa caaccacaag gctcaacaga
    13441 aaaaagccaa cagtgcgcgc gtgaattttc cagggcaact gcttaagcgc ctggattaac
    13501 cgcatcctct tcgcggttct tgctttccgc ctgttggctc tatctcgagg ttccaccatg
    13561 ctcggtatgc ctatctcttt tctgccacgc gcaatttcgc gcttctggcc cgaacgttgc
    13621 tgcttacttc tatcccgtca ggggtgatgg ggtgacgggt gacttcaatg agcgaagctt
    13681 tgtgcccgca gatacaaaca ggttggtctg cggggcagat acaatctctg ctttcacgcc
    13741 tgaaaaactg cttgacaatg cggtcttcca gcgagtgaaa agaaatgacc gcgagtcgac
    13801 cgccgggtgc cagcgactct accgcctggg gaaggaaaat ctgcagcgag tccagctcat
    13861 ggtttacggc gatgcgcagc gcctggaaag tccgagtggc gggatgaatt tcgcttcgta
    13921 tgccgtagct ggcttttttt atggtttctg ccagctgcaa ggtgctggtg aagggtcggt
    13981 tttgaacgat gacacgcgcg atgtggcgtg aacgccgttc ttcaccgtaa cgccagatca
    14041 ggtctgccag ttcggattca gtggagtggt tgaccagtgt ctccgcgctg acgggctggg
    14101 acggatcgaa gcgcatatcc aatgggccat cgttgagaaa agaaaaaccg cgttcgggag
    14161 aatcgatttg cattgaagac acgcccagat cggcgacgat gccatctacg tggtcccagc
    14221 ctaacgtcag tagcgcgttg cggagctcgg tgtaggagga gtgtagcaaa tgaaagcgct
    14281 ggtgaaaaat ggagaggcgc tggtgagaaa gcgccagcgc ctgagggtca gtgtctaagc
    14341 ctagcaactg tccgtctggc gagctcttct ccaggattcc ccaggcatgt ccagcggcgc
    14401 caatggtcgc atccacgtat aaccccgggc ttacaggacg taaagcagtt ataatttgat
    14461 ggtaaagaac tgagacatgc ggtggcttgg ccgcgctgtc gtgtgcgttt ttcatcagat
    14521 ggtcaggtca aaactcgaga cgagttctgc tgcagaagaa ggtccttcac cctggtcctc
    14581 ctgaatcttg taggtgtcgg ggttccagat ttcaatgtct ttaccaatac cgacgatcaa
    14641 agccgtacca tccaacccag caattcggcg taagggttga ggaatgataa aacggccggc
    14701 cttatcgaac tcgatttgag cggcggtaga gaagatatat cggcgtaatt ggcgggtttg
    14761 ggcgtttgcc atattcatat tgttaatgcg ttcatagagg agttcaaaac ggttggcatt
    14821 catcacgagc aggttctggt caaatccctt gagaatgaac gggttttctc ccaggaggtc
    14881 gcgaaaagag acagggaacg ttaagcgccc tttttcgtct atattatgaa gataacaacc
    14941 ggtgaaaccc ttgaacataa tttctacctt tttttattat ggcgaacgcc ggaaagaaat
    15001 ccggcgctcc acttattccc acttcctacc actttcctcc acattataca gattattttg
    15061 tacaaatgca agtgtttttt acgtttttga tatgcaatta agataaattg gtgcaggttt
    15121 tttgccaaaa atagacaagg agcagacaat gagatcattt tttgcacgat tttcaatcct
    15181 ggtggtcgtt ttactggggt ttactatgct gaacgggtgt attacgatta atctgggtgg
    15241 agcaacgccc gactccggac aaacactcga gccagccccc acaacacctc cgccggctgc
    15301 tgtgcaaact ccccggcaag ttttaaatga agagcggctg ctgaacgcag attatctttc
    15361 cccgttgttc caggtaccta ttaccctggt tggcggggtg ttttcgggag tggtggacgg
    15421 ctccgagctg aaagtgactc tgcaaccggg tattcagtat ggagacctga atgatgatgg
    15481 ggtcgaagac gcggccttca tcctggctga aagcactggc ggcactgggg ttttctcctc
    15541 tttggtggtg gtcttcagcg aggcggggcg atttgtgcag gcgccaggcg tgatgatcga
    15601 tgaccgcccg gtgatcgagt cgctgaccat tgaaaacggg gtggtcaagt tgcaggcgtt
    15661 ggttcattca tttaatgaca caatggtcaa tcccagcacg cgcatccgcg cagagtactc
    15721 actgttggac ggcaagctgg tgcaaacccg gctctcttct gctttctctg gcagcgagga
    15781 gcacgtgatt accattgatt caccgcgtga tggtgatact ataagtggca gtgtgcgcct
    15841 gatcggttcg atgccgattg gaccgttcga gaacactctc ttgttgagga tcactgacct
    15901 atcaggtggg gagctgctgc atgaaggatt catggtgcaa acggaagacc tgggcgctcc
    15961 ggcgactttt gataacaccg tgctcatccc ggggattgcc cccggaacgc ggattttgtt
    16021 gacgttgagc gagttgagca tggcggatgg ctcgccgatt gcactggatt cggtattgct
    16081 ggtggtggga gaataactcg cgactggcag catattcgct gataatcccc tgtaagatac
    16141 tggaggtcag acgaaaaaaa acagggcgcg atatgccgcg ccctgttgaa gactgttggc
    16201 ataagtgtca accatcaaat tcaccaacca gcatgtcatt tacgtagaca aggtaatgtc
    16261 cataaggata agcccccagc ggtacgctgg tttcaaattc ttgaagcacc tggatacaca
    16321 cgaccgctgg atcaacaagc gaatacacac tgatggcgat atggttttcc ttgtcaggcg
    16381 gggagatggc gacgcgcaat atgtgacagg gcgtaggcag gctgcctttt atttgaaggt
    16441 tgacctggat ggggtcgctt tcaagaatca tgagctgggc gttgtcgacg aacgctgcgg
    16501 aacgctgctt caactcatca ccaggaccag gttcaaaaga gttgggcgtt ttcaatggtg
    16561 tcaaagaatc aacgggttgg tctggcttgg cgatcgtagg ggccggaggt gaggggtttt
    16621 catcattgat tactgggcga gccgttgcgg tagacagtgg tgaaataaag tcaggggtgt
    16681 cagacagcgt cggattgcag gatacaagaa gcagcattat tgagatggaa aaaatcaaaa
    16741 agcgcatggt ccctcctggt caatcaagtt atagagttga cgctttctca gttgcgattg
    16801 ttccgcgttg aggttgtgag gttttcaaca agcgccaggc ttatggctgt gattgacggt
    16861 attcccgcca gcccccccaa cgggtgattt cctgctggcc agaaataaga taaggctctg
    16921 cgagcacacc aaagaccaat tcaccaccct gcagctcgac gcgccccagg cataacccgg
    16981 gcggttcctg ttgcagcacg gcaacgagcg cgcttgctga gagcgaccat atttccacgt
    17041 ggatggcagc gcccaaatga tcatcacgca gcatggcagg gtagagatcg ccaatcgacc
    17101 atagacggta agtgggtgcg gtgacagttt ggcacaggaa gaccgctccg acggagagca
    17161 ggttgtggtt cagttccaac ccgcgcatca gggtgccgtt gactgccagt tttacttttc
    17221 tcattagcga aatcctaaac atcgctcaca cgcggccggt attgcagcgc ttcagccagg
    17281 tggatggtag agatgtgcgg gctgccagcc aggtcggcga tagttcgagc cagcttgagg
    17341 atgcggtgat aggcgcgggc ggaaagctgc agctggttca tagcggtgcg catcagtccg
    17401 cggcaggatt catcgagaga gcagtactgc cgcacctgcg ccggatgcat atccgcgtta
    17461 gaggtcaatg acgtatcagc gaaacgctcg cgttggattt gacgcgcgct ctccacccgg
    17521 gtgcgaatga cagcggaggg ttcacccagg cgtgcgtcgc tgagcttctc gtaatccacg
    17581 cgcggtactt ccacgtgaat atcgatgcgg tccatcagcg ggccggagat gcgccgctga
    17641 tacttggtga cgatgcccgg cgcgcaggtg caggcccggg tagggtctcc gtaataaccg
    17701 cacgggcagg ggttcatggc ggcgacgagc tgaaaattgg ccgggaaggt gagcgtgccc
    17761 tgcgcgcggc tgatggtaat tattttgtct tccagcggct ggcgcagcac ttcaagggtg
    17821 cggctgccaa attcaggaaa ttcatcgagg aagagcactc cacggtgggc caatgaaatc
    17881 tcgccggggt gcggccagtt gccaccgccc accagacctg cgtggctgat ggtatggtgc
    17941 ggcgcgcgga aggggcggct cttgagcagt gggataccct caggcagtgc atctgccact
    18001 gaatagatgc gggtaacatc gagggcttct tcgagcgtca tggagggcag gatagatggc
    18061 agggcacggg ccatcagcgt tttgcctgcg cctggcggac caatcatcag caggttgtgc
    18121 gcaccagcag cggcgacttc gagggcgcgt ttgacgtgtt cctgtccctt gatctcgcgg
    18181 aaatcagtct gtaccagcgc gctgacctgt tccactgcca aggcaggctg tgggtcgatg
    18241 atcacctgac caacaagatg ctggaaaagg tcagccagag aactgaccgg gatgacttcc
    18301 aggtcgggga agatagcggc ttccggcgcg tcctccgcgg ggacataaac gcgcttataa
    18361 ccctgggcgc gggcgacggc ggccatgggc agcactccgc ggatgtggcg cacgctgcca
    18421 tccagcgaga gttcgcccat caccagggcg tcttgcaggc actgcggagg tagttggcgg
    18481 ttggcgatga ggatacccag cgcgatgggc agatcgtagg ctgagccttc tttgcgcacg
    18541 gtggctggag ccaggttgac gcgtacgcgt ttgcgcggat actccagccc agtgttgcga
    18601 atggcggatt ggacgcgctc gcggctttcc tgcaccgaga catccggtaa gccaacgatg
    18661 atgatgggcg gtttgatgcc gggtgtgaga tcgacctcaa catccaccac gaccccatcc
    18721 aaaccgatga cagcgcaggc gttcactttg gcaagcatgg attaagtgta gaagtggaga
    18781 gggaaaaagt caatcatctt tttactggtt aatccctttt catcagccct tgtgaaagga
    18841 ggattgccct ggcaggcggt cttggcaata tctcgacaag atgcgaacca caggaaaaat
    18901 cgcgtagcct gatatggtga aaagcatgtg agcgaggaga aaccaggaca cccttgcctg
    18961 agttggaaag gctagcgggc tggctgactg actgaccagc tggtggtcgt tttatgtagt
    19021 atttattatg cagctgctgc cttaaaagat atctgcacag ttggactggc agcaagggta
    19081 gccgccactc ggtcaaccga gcagtttcct cacccgccgc agcatgcgcc ggtcatcttt
    19141 cagtagaccg gcggcgggca ggcgttcgag cagcagcagc cagttgggat tggcttgaaa
    19201 cacacgtttg agaatgggca gggcttcctg ctccctgccg ctatccagca aggtgaccgc
    19261 ctgccagaag ggaagttcat gcaaatgcgg agcgagcgcg gcagcctgct cgtaggcgct
    19321 aaaagcctga tcgataagcc cctggctgag cagctcatca ccgcggttca tatgttcata
    19381 agcatgttga acggtgagca ggcggcgtaa ttcaaccagg ggttcggaat ggtcttcaac
    19441 acgcaggtct accagtaccc ctttccatgg ttcgttatgg ctctctcctg ataccaccag
    19501 taaagcggcg gattgttgac cgcgaatatc tcctccctga gcctgggcag catccagcgc
    19561 ggcgagcagg cggtctgcaa gcgaaccctt cgccgcttca tatgcggcgg ccatcgccgg
    19621 ccagaccgta ttacgcagca tcatattcgc ttgcacagaa tactggtaac cagtttggtg
    19681 accagcttcg gcgatgcaac gttgccctgt atgcaccgct actgtgcctt ttccatctac
    19741 catggccacc tggcgcactt cacggttggc gtcttttgcc agcaggtttg caagaacaac
    19801 ggaggctggt ttgccttcgc gcatcgcgct caatcccagc gggccatagc tgacttcagc
    19861 catggattgt gtagcgatgg cgcctacacc cggctctgcc caggcaacga tggagcccgt
    19921 tgaaaaccaa tgcgattgca cagcaacgcc caactcaccg ttcttggggt ctcgggcgac
    19981 aatggaaaag gtgtgcgcga aaggaaaacg tctctctggc atggaaactc cgattgcggc
    20041 tggttctgat tcagcccatt tcctggtgaa tatccgcgcg gcaccatcgc gcgaatatcg
    20101 cggtggagag gaagcgtttc gctgaccgct ccagcaagcc aatctctcca gcctgggtaa
    20161 tgcctttaaa cctggcggtc atttcgagaa tggcgttgta cattgttgcg gcagaagcct
    20221 taacggcgta cgcggtgagc tgaacaaaaa gcgggtcagg ggagaggatg gcgcggcagg
    20281 cggcgagcag gtctggcagc agcttgtaaa aatcccatat ttctcctttg ggtccacgcc
    20341 cgaacttggg aggatcaaga atgagagcat cgtagcaact gccgcggcgt tgttcgcgct
    20401 gaacaaactt aagcgcatca tccaagatcc aacgaatcgg gagtgaatca agcctgctga
    20461 gggtttggtt ttcacgtgcc catgtgataa ccttgcgcga ggcgtccaga tgggtcacct
    20521 gtgcgccagc cgccgccgct gagagagaag cgatgcccgt gtagccgaag aggaccagca
    20581 cgcgcggctt cccgcctgac aggtggatga ctttttctgc ccagtcccat tggcaggatt
    20641 gttcggggaa gacacccagg tggcgggagg aagaggtttt aacccaaaaa gcgagttgcc
    20701 catagctcag cttccattga tcgggcattt tcttacggaa ttcccaatga ccaccatttt
    20761 cttcgggggc gggtttataa agcgcatcgg ctgcccgcca ctcattttct ggtaaggctc
    20821 gttgccaaac tgcttctgct tcgggacgga tcaagcgata cgctccgaag cgttccagct
    20881 tcatcccggc gccgctatcc aacagttcat aatcctgcca gctggtcgcc gctaacaagc
    20941 tcaggtcagg aatcgtcatc gggtgttcca tataaaaaag tatatcagac ttgaccattt
    21001 gagcgcgtat caatgcgggg tggactataa tccatcattg accaagaaag gagcagcgaa
    21061 acgatcatgt tccaagcacc tgaacgcagg aataccgata gtgttaaatg gggattgttc
    21121 accgaagacg tattgccctt atgggtggcg gacatggatt ttacctcgcc cccggtcgtt
    21181 attgaagcgt tgcagcaacg ggtaagccat ggcgttttcg gctacgcact ggaatcacaa
    21241 gagcttaagg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngatgaaatc
    21361 cactgcgacc tggtgtacaa agggtaccag catattccga tcgcttcgct ggatgatgag
    21421 attgctgcca gcacggtgac actcatcgca ccaagcaaga ctttcaatat cgccgggttg
    21481 gattgttcga tcctaatttg ccagaacgaa gagttacgca agcgtattct ccattccaaa
    21541 cgcggactga tgggcggggt gaacctactg ggaatgactg cggctgcagc agcctatcgt
    21601 gaaggggctt cctggctgag cgaggtgctt gccttcctcg agagcaaccg agagcacttg
    21661 atggcttaca ttgagcacaa cctgccgcgc atacatatgg tcaaacctga agcgacgtac
    21721 cttgcctggt tggattgccg ggagctggaa ctcgcaccaa gcccgcgcga atattttttc
    21781 caacgtgccc gggtagccct caatgacggt cgagattttg gagaacccgg taccggcttt
    21841 gtgcgcttga acttcggttg ttcgcgtgag acactggata acgcgctcga acgaatgtgt
    21901 cactcactct cttaaaagaa aaaagcagag tcgcaaggct ctgcttttaa gtggtattcg
    21961 atttttgatt attctacagg tttaacttcg gcagcctgca aacctttagg acctttctcg
    22021 atggagaatt ccacctgttg cccttcctta agaactttgt atccctccat ttctattgcg
    22081 gaaaaatgca cgaagacatc ttcggcgttt tctcgcccga tgaaaccgta cccctttgcg
    22141 gggttaaacc acttgacagt tccaacaaaa cgttcagcca tcctaatact ctccatctta
    22201 aatagtgtta gcggaagggc gaactcccct cggcgccttt gatatggtga atttaccata
    22261 cgcataaact ggtgtcaagc aaatgaggag cagcaaaatt ttgtgcgaag cgggtggcat
    22321 ataataaagc tatgaggaat gaacgtaaat tatggttgct gttctttttg ctcctgccat
    22381 tgggatttat ggctttccca aaggcaggag cagcgcctga aaaccttacc ctgtctttgc
    22441 aaaacgaccg ggcagcgctt gtcaatggag atgcgcagat ggaactcacg gcgattcaac
    22501 aggcgctgga attccagacc tggcggggag acttgtggca gcgcgcagga cgattataca
    22561 tggatcaagg ggattaccag aacgcgctgc attcgtttga gaaggctcaa gaacttggtc
    22621 agcttgaacc gttagggcag gtttggctat ctgacatgtt gatcgccaac ggttatagcg
    22681 acgcagcaaa atatgtattg caatccatcc atacagacga tgtttttatc ctgcttcaag
    22741 ccgctgctct gatggcgccg ttaaacgatc acgagggatt gcgccaactg ctgcaaaagg
    22801 cgtacgcgtc tgaaccaacc aacagtgaag tgaattacca gatgggtatc tatttaatga
    22861 cagaagaccc aggccgtgcg gttgcgcact ttgaactggc taaaagagac cagtcccgta
    22921 aggcggctgc ggcttacctg gttgagaaca ttcaacaata tggcgctctt gaggaccacg
    22981 gagaatggta tctctacgct gggcaagcgc tggcgcgggt taatgaatgg aacgcggctt
    23041 ttcaagcctt ctccaaagcg gtgcagtcgc tgccagacaa tgccactgct ctggcgttgt
    23101 taggagaatc ccagttccaa cttggattgg atggggggca aaacctcgaa aaagcctggg
    23161 agctggaccc tgaaggagaa ataaccaacg gagtgttagg gatctattac cagcgccagg
    23221 gcaacctgga aaaagcgctg gagcacctgc gaat
//
LOCUS       DAOSPM010000024        21316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2475_length_21316_cov_15.830394, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000024 DAOSPM010000000
VERSION     DAOSPM010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 21316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21316
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2475_length_21316_cov_15.830394"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..377
                     /locus_tag="PLW25_03635"
     CDS             12..377
                     /locus_tag="PLW25_03635"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HOS53613.1"
                     /translation="MKTRPAQFIDEPIQVEFDSPPTFDKKPPCPQRFRWRGQVFTITS
                     VVEERVDYARRGRMSRNMAPAHLASASRSGSWGVGRFYFCVMVDSGQIFDLYYDRAPK
                     DADNRMGGWFLKGERLSQS"
     gene            581..1009
                     /locus_tag="PLW25_03640"
     CDS             581..1009
                     /locus_tag="PLW25_03640"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOS53614.1"
                     /translation="MINKTLDQCPVCESELMITRLYCVNCDTTIEGHFAAEHTPFGNL
                     TPEQMQFLLTFVKCEGRLNRMEDELNLSYPTLRSRLTEIVRALGYEPGKDEGPAQLSP
                     EGRRDILEKLDRGEISWEEARAILRGEKFIPQHNAAEGGR"
     gene            1009..1404
                     /locus_tag="PLW25_03645"
     CDS             1009..1404
                     /locus_tag="PLW25_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53615.1"
                     /translation="MATGEERLKVLKMVQEGKITAEAGVELLKALDAASKKAEVDERA
                     AVNFPGKGGGKFFRVRVTDTDTGRTRVNIRLPLGMVNAGLRMGMRFSPEVEGLDAARL
                     VEALASGETGKIVDVLDDEDGEHVEVYIE"
     gene            complement(1701..3206)
                     /locus_tag="PLW25_03650"
     CDS             complement(1701..3206)
                     /locus_tag="PLW25_03650"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOS53616.1"
                     /translation="MMQKKVERFKRMIMEVTDLGHAEAVLGWDQQVYMPRGGGEDRGD
                     ILETIASLAHQKFTCNEMGELLAELKPYAETLDADSDDACLIQRVAHRYDKETRVPTE
                     WVVDFTRVTTLAQMAWEEARAKSDFTIFQPHLEKVVDLRRAYASFFQPYDHVYDPLLD
                     GFEPGLKTAEVQQIFDALRPQQVALLKEIAAKPEIEDAFMHVPYDEQGQWDFGVEVIT
                     RFGYDWNRGRQDKSVHPFTTSFGINDVRITTRFDPSRAASALFSTMHEAGHAMYEQGF
                     SASLRRLPLADGASMAVHESQSRMWENLVGRSKAFWKFFYPRLVQKFPQQLKGVSLEQ
                     YYRGINKVEPSLIRVEADEATYNLHIMLRLELEIGLMDGSVRVADLPGLWNKKMQEYL
                     GVTPPNDRLGVLQDVHWSGGMIGYFPTYALGNLVSAQLWEKIMENIPDLEDQISRGKF
                     DDLLAWLRTHIHRHGAKYEPQQLIKKVTGTGIRPDPYMRYLRAKYAEIYNL"
     gene            complement(3221..4162)
                     /locus_tag="PLW25_03655"
     CDS             complement(3221..4162)
                     /locus_tag="PLW25_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOS53617.1"
                     /translation="MAAWKVLLTDGLEENGKEILRSAAEVTDQSGISAEDLLKVVGQY
                     DALIVRGRTKVTESVLAAAPHLKVVGRAGVGVDNIDLAAAKAYQVTVVNSPLATTVAV
                     AELTLSLMLSLVRELPRADASMKAGKWLKKEFEGRELFGKTLGVIGFGRIGSAVAARA
                     KAFEMKILAYDPLVSAEEIKKRGGEPVSLDELLTAADMITMHMPLTADSRNLLNAEAL
                     AKTKAGVYIVCAARGGVIDEEALLAALNSGHVAGAALDVFANEPPGATELVAHPHVID
                     SPHIGAQTVEAQARAANDIAEEILNALAGKPLRWKVA"
     gene            4285..4899
                     /locus_tag="PLW25_03660"
     CDS             4285..4899
                     /locus_tag="PLW25_03660"
                     /inference="COORDINATES: protein motif:HMM:NF022751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOS53618.1"
                     /translation="MDATTKKALSRVNPRLLQSEAIQRCSLADCKGTCCVFGVWVDLR
                     EVDDIMRNAAIILPHMPPDCRVPGEWFAAVEDRDKRSPSSRVIHTAVENRAEHYQGTA
                     CVFCLADGKCALQVAALANNLHPWRFKPFYCVLHPLDLDEEGRITLDAAEDMVNEQGS
                     CLRRAPKPIPLLDTFEPELKYLLGEQTFSCLKQFTSRNTEDEPE"
     gene            5002..6171
                     /locus_tag="PLW25_03665"
     CDS             5002..6171
                     /locus_tag="PLW25_03665"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOS53619.1"
                     /translation="MKPRLSLAVLILTAVFAALFARVQGVSALTSPGKSEADSALCLP
                     ETSEELMNGCLKAGPAARLEELAARGIPPQGRQLFASRTPYELGATPFSYAKLVDDQE
                     IPLYATLADVESGKPSNVLPKSRTKYVALMNSAETSRGRFYQIATTEWVSVELAKKVA
                     ATNFQGYILKESPDFSFGWIINETTSRAGPGYEYPETGVNYWRLDPVMVYDSAVVNET
                     EWVMIGYNEWVEHRVISRVIPNYQRPAGVPVDKWIEINLYENTLTAYENGQMVFATLI
                     ASGVNPFFTQPGVFQIYKKLESDPMSGSFEVDRSDYYYLEEVPYIMYYDKARAIHGAY
                     WQPFLGYQNSHGCVNLSVADAHWMFNWANDGDYVYVWDPSGKIPTDPSFYGDGGF"
     gene            6265..7047
                     /locus_tag="PLW25_03670"
     CDS             6265..7047
                     /locus_tag="PLW25_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53620.1"
                     /translation="MKIKKWIFIAATTALMSVAGSACAPAAAIESTPQPTQTPYVIVV
                     TPTAAEVSATPEPTEPLAAPDAETPRGEEPPKYFREEFDGGLDDFEQILWTGRFGKDK
                     IYTEDGKLKVRLNAMYLAPYLTYDPYTYTDVRLEVSAENLAYNDNHITLVCRYDEELG
                     WYEFNISSNGLFRINYYDNTLVKGHVKLWNGGSNDIKMGRQINKITAECIGTQLSLYV
                     NDVLLKTVEHKDLREGRVGLGVSSFNQFPVNVDFEYFDISQP"
     gene            7175..8074
                     /locus_tag="PLW25_03675"
     CDS             7175..8074
                     /locus_tag="PLW25_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53621.1"
                     /translation="MNKKAFISLMMIALALTLLVSACGNSKGAATTEPPQVIIITATP
                     ESPQVIIITATSEEMAPPIVATEPPAAPPTEPPDAATRPTPPFGYEPTPFIAEDSAQG
                     ERYFLDEFDNGLDQYDHFIYDITVKVNKIIKENEELEKKIKVELEDGTIEFNIERYYL
                     NYYMIYRPQVYEDVKISVEVENLGFPASQAALVCRYDEELGWYELIVSNTGRWRLYYF
                     DKPLVKWNFLSDGGAEKFHYGKDTNKYTLECKGKELSVYINDVFSQTVTNKNLKKGKV
                     GFSIQAPKSWTTMNVPWFEVSEP"
     gene            complement(8143..8919)
                     /locus_tag="PLW25_03680"
     CDS             complement(8143..8919)
                     /locus_tag="PLW25_03680"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOS53622.1"
                     /translation="MERFAGSQEEQQPQPPPVGTPVTVRLPVRKPWVTYGLIGITIAV
                     FLLQLLSQSTSHDGVDWPFLLGGKINAYILRGELWRLVTPALLHGSLLHIAFNMYALF
                     SLGSSLERYYGHGRFLVLYIIGSFCGNSLSFLLSPNPSLGASTAVFGLVAAEGVFIYR
                     NRRLFGSRARAMLSNLVLIVVVNLLIGLQPGIDNWGHLGGLAGGALFAWMAGPLLLVQ
                     QTSTGYELQDSRLSGGLLWGILLSAGLFSAMVISRFITGD"
     gene            complement(8919..9542)
                     /gene="gmk"
                     /locus_tag="PLW25_03685"
     CDS             complement(8919..9542)
                     /gene="gmk"
                     /locus_tag="PLW25_03685"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOS53623.1"
                     /translation="MSSEEVSFDLIHPQPLFIVISGTSGVGKDSVIRGLKQRDLPLHV
                     VITATTRAPRAGEVDGKDYFFFTPDEFKTRQAEGWFAEHAVVYSDLKGIPRHQIEEAL
                     ASGKDVVARVDVQGARTLRKQYPQAVLIFIVPGSFDEWYQRLTRRGTESADDLRLRVA
                     KAREEVKQIGLFDYVVVNPDGQIEKAVDDVVSIIKTEHMAVKHRRIL"
     gene            9626..9991
                     /locus_tag="PLW25_03690"
     CDS             9626..9991
                     /locus_tag="PLW25_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOS53624.1"
                     /translation="MPIYDFRCSSCKQRFEVFLTYAEYDHHQTACPSCGSTDLQRVIR
                     KVRVARGDAGRLESLANDSDLDMIDHDPKALGRMMREMKNEVGADDLPGEFDEVVSRL
                     EKGQTPEEIERDLPELGEE"
     gene            complement(10030..11325)
                     /locus_tag="PLW25_03695"
     CDS             complement(10030..11325)
                     /locus_tag="PLW25_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53625.1"
                     /translation="MPSLNDNALSVNRYNLRMTWRKYTGIAVPLLMMMVLLCGSVVSL
                     ADPLDQARLFTADIEYDYIAWLANAAGQKASQALLNPVQRLPQEQQTRLVKRYFALVQ
                     ALEIVQAKVELVYADPAISDAAAAAAELLRQQHALQQSLDGLSPLVEAILQFQVARVM
                     ADEGIGFLAQPLPPVLFHSSPLPKALIVSPRDSIRQEVNISLLADLGLEDITRLEENV
                     SSYMNASALVVDVGGIGVYPTMVQRSSNIEWTLDTIAHEWTHNYLTLHPLGWNYDTTA
                     ELRTMNETAASIVGKEISREVMRRYYADDDTGENKTPKAWLKRTAVNEFDFRQEMHKT
                     RVRVDELLAQGKVEEAEEYMEQRRVVFVEHGYMIRKLNQAYFAFHGAYADMPGGAAGE
                     DPVGPAVRALREQAGSLALFLRRIAWMNSFTDLQKALAH"
     gene            11347..12756
                     /locus_tag="PLW25_03700"
     CDS             11347..12756
                     /locus_tag="PLW25_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS53626.1"
                     /translation="MTKKTILFILLGLAAAGVIAYLVLNTLSARKAAVAELQTVEVTR
                     GELVAIIGATGTVRANQSAVLVWQTSGRVGTIHVKTGEKVAGETILAELIESSLPQNI
                     ILAQADLVEAQRALDNLLDSNLSQARAQLNLANAKDAYERAVWSSWHGDTARTTNQNK
                     IDAAQAAVTLARDRVNTAEENYDKVDERDDDDPLKANALSTLANARESLDKAIKDLDY
                     LVQNPNEKEIAISEGKVAVALAEYEDAQREWERLKDGADPNDIAAAKARIAAIKATMA
                     LAHISAPFGGTVTEVNIMPGDLVTSGGVAFRLDDLSHLLVDVQVPEVDINRVVVGQPV
                     ELTFDAINNQAYQGKVNRVARVGTIESGLVNFKVTVEILNPDEQVLPGMTAGVNIIVS
                     QLEDVLTVPNRAIRLVNNHPVVYILKNNIPTEVSITIGASSDSMSEILSGDLKEGDKV
                     ILNPPSSFMDVHFSGRPPF"
     gene            12766..13446
                     /locus_tag="PLW25_03705"
     CDS             12766..13446
                     /locus_tag="PLW25_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53627.1"
                     /translation="MEPVIQTHELTKIYQLGEVSVHALAGVSITIDRGEIVAIMGPSG
                     SGKSTLMNLLGCLDRPSSGTYTLDGEEVAQLDDDHLANIRNRKVGFVFQSFNLLPRQS
                     AIENVELPMRYAGVTKEKRERAIAALESVGLADRMHHRPPELSGGQQQRVAIARALVN
                     NPAILMADEPTGNLDSRSGQEIMELLLKLNQNSGTTLIIVTHDQGVASNTQRVIRLKD
                     GLVEGDTP"
     gene            13443..14669
                     /locus_tag="PLW25_03710"
     CDS             13443..14669
                     /locus_tag="PLW25_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS53628.1"
                     /translation="MNLWQAIVEALESLYSNKLRSFLTILGIVIGVGAVIAMMAVGAG
                     AQETITGSISGIGSNLLFVIRGNPTVELTRIEPLTSEDAEAFLDQFQAPSVAGVAPVV
                     NGSLVVSTAGESTRTTVYGVTPAYQQIRNYGLTEGVFISEEQLLGRSSVALIGPDTAE
                     KLFGRREGLVGETLRIEGQPFRVIGLLETKGGSSFGSQDDLLIIPLSTAQARLIRHPD
                     NRLDMILVQVISPELTTQATEEIAQILRSRHRTRIGQDDFTIFSQDDFVDLAKTITGV
                     LTIFLGGIAGISLLVGGIGIMNIMLVSVTERTREIGLRKALGARKKDILIQFLTESSL
                     LSLFGGIIGIGLGWLISYVVGRIAVANNTPFTPQVSLDAILLATLFSTAVGLFFGLYP
                     ANRAANLEPVEALRHE"
     gene            complement(14745..14942)
                     /locus_tag="PLW25_03715"
     CDS             complement(14745..14942)
                     /locus_tag="PLW25_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOS53629.1"
                     /translation="MDNNTKKFYRSRVDRKIGGVCGGLGEYFGIDPTLVRLLFVLGLL
                     FVGGTLLAYLILLIVIPEEPL"
     gene            15069..15344
                     /locus_tag="PLW25_03720"
     CDS             15069..15344
                     /locus_tag="PLW25_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53630.1"
                     /translation="MAEKTQAEKHQIIHLLQQIPFDETEKALWMENVEENGVTEELLD
                     EMHTKLLALPQEKFASEWVKAKLSTDLARVARQWRMQRASRQFKHNR"
     gene            15417..16316
                     /locus_tag="PLW25_03725"
     CDS             15417..16316
                     /locus_tag="PLW25_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOS53631.1"
                     /translation="MNQDTEKINLRERLVSDTYLLLAAIIWGAGFVAQRMASFHLGFN
                     AFNGIRFLLGGLVLLLFVWRRFRAFKGGWGWVLLAGGVLFSASSLQQIGIGTTSASTA
                     GFITGTYVVLVPVLLALFWRQRTPVVTWVAALVALAGTYLLSTGGTVLIPSTGSLILL
                     AGSVVWALHVIVVGLAVKKIDVLVFSVGQFLVCGLIHLVMSMFIEPVSMPALRASWLP
                     LLYSSLFSVVLGFTLQAIGQKKAPSADAALLLSLEAVFAAITGVLFLNEQLNLVQVIG
                     CVVILGAILFAQLIVLKQNRKPL"
     gene            16407..16799
                     /locus_tag="PLW25_03730"
     CDS             16407..16799
                     /locus_tag="PLW25_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOS53632.1"
                     /translation="MELIDRQALIDRIRVLQHSLPEVQAAAVISLDGLIMASALPEGM
                     SEDRISAMSAAMLSLGEQINKETGLGTLEQLYTRGSNGYVVLLAVGKEAVFTTLVQPE
                     AKLGILFLELRKTAEDLARLLNGNGLNT"
     gene            16905..17639
                     /locus_tag="PLW25_03735"
     CDS             16905..17639
                     /locus_tag="PLW25_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOS53633.1"
                     /translation="MSICVIIPTYNEVENLPLIVARLRALPLEDLSILVIDDNSPDGT
                     GALADELAERYKGQISVMHRKGKLGLGTAYISGFKQVLQTNTAYIAHLDADFSHQPEK
                     LLEFMKSIEGSDVVFGSRYTPGGSLDRHWPAWRKWLSNFGNTYARTILRIPTKDLTGG
                     FRLWRRETLAAMPWDRVRSNGYVFMVETAYLAHRLGFKIKEVPIYFAERTRGKSKMNF
                     SIQVEAALRVWSLISRYRDIQPLPRG"
     gene            complement(17636..18472)
                     /gene="rsmA"
                     /locus_tag="PLW25_03740"
     CDS             complement(17636..18472)
                     /gene="rsmA"
                     /locus_tag="PLW25_03740"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOS53634.1"
                     /translation="MKRHHLDPRKGLGQNFLVDHHALRRIVKSAELTGGEEVLEIGAG
                     LGSLTRLLAAAAGVVRAVEIDHRLMPVLREVLSSYANVELVQGDILALDPARLMNEPG
                     YIAVANIPYYITSAIVRHLLECPKRPARLVLTMQKEVAERICAEPGDLSLLALSVQVF
                     GQPSISAVIPAGAFYPVPGVDSATLRVDLHPQPYFTDEQRKMFFSLIHMGFAQKRKTL
                     RNNLAAGLHLSGEEAARYLVDADIDPQRRAQTLSLVEWKTLVNELKDLLGAKREQETP
                     PA"
     gene            complement(18502..19893)
                     /locus_tag="PLW25_03745"
     CDS             complement(18502..19893)
                     /locus_tag="PLW25_03745"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HOS53635.1"
                     /translation="MKRKHWPMLMIAVPIIGLVVGYLLMTRNVPATIDGTPLTIRTHA
                     LTVGGALRSAGFAVDEGDAVFPAAGTWLSKADEIRLDHARLVRVYDTTQKQPIELSTS
                     ALTAAEVFKQAGLTLGSLDSLTLNGLPVGPNEKLPFGREMTLNYRPAVQVRLNVDGRD
                     TTITSSAGTLGEALWQAGFRLREGDRVTPGMDTALTENLAVEIHTARPLSISVDGTIL
                     EGYSAAPTVAAALAENGVTLQDIDTSQPGAGDPLPEDGVINVTRVTEEIHAEQRVIPF
                     EIESLADDTMFINETNITQAGQNGLAATLVKVRYEDGLEVSRQTQNEVILIPPVAQVE
                     YRGTKMLENYIDTPEGALQYYLVIDAVATSFSPCRQGIEGYCSNGTASGRTIQKGVVA
                     FKRNWFNLFRDTRVYVPGYGIGVVGDTGSYPYSDSWIDLGYSDADYVPWGAVKVKIYF
                     LSPPPSGFTGVLP"
     gene            complement(19966..21144)
                     /locus_tag="PLW25_03750"
     CDS             complement(19966..21144)
                     /locus_tag="PLW25_03750"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOS53636.1"
                     /translation="MITRIKQGMLIFLVACLLISCTNTAKISASDKVNTSVAQTVAAA
                     QPTGGLPLPDGAVQPTNPAPPPVEQPTTTPPPLPTLAPSIPTLPPSPTTDTCNQAKFV
                     QDVTIPDGTKITPGLAFTKTWRIQNTGTCSWNTAYAVVFDSGHSSGAPTVTPLPANVP
                     PGGTIDVSVVITAPTTNGDYTWKFMLRSDTGRIFGFGAGYAYPMTAVIKVEPLILLTA
                     LPHFTLIPIVFPKYDFTANYCSATWSNGHQDLPCPGTKNDSTGFVVQQDKPRLQDGNT
                     RDTKSLLTHPEWVDGGAIVGIFPAVSIESGNRFKTTIGCGYSGTSCDVLFRVNYKKGS
                     DPFVELGTWSMKYSNAPLDIDIDLSSLAGSDVKFQFIVFANGSSSQDWAHWVFPRIAK
                     "
BASE COUNT         4953 a         5937 c         5648 g         4778 t
ORIGIN      
        1 aaaggtgaag aatgaaaacc cgcccggccc aattcatcga tgagcccatc caggtcgaat
       61 tcgattcgcc gcccactttt gataaaaaac cgccctgccc tcagcgcttt cgctggcgtg
      121 gtcaggtgtt taccatcacc tcggtcgtgg aagagcgggt ggattacgcg cgccgcgggc
      181 gcatgtcgcg caatatggcg ccggctcacc tggccagcgc ttcgcgctct ggttcttggg
      241 gtgtggggcg gttttacttc tgcgtgatgg tggattcagg gcaaattttt gatctctact
      301 acgaccgcgc ccctaaagac gcggataacc gtatgggagg ttggttcctc aaaggcgaac
      361 gcctctccca gtcataagca gaacaaaagc gcctgcttgg ttatcgtcca atattaatta
      421 ttcttattca tatattgaca ttattgatta tcatggtaga atatacttaa tattattaag
      481 gtttgattgg tattttagaa aacaaagtcg tgaacaactg gcgagaaccc ccccccgcag
      541 tgcgcgtttc tttctatcaa accaatcgaa ggaaaatacc atgatcaata aaacattgga
      601 tcaatgcccc gtatgtgaat ctgaactgat gatcacccgc ctgtattgtg tcaattgcga
      661 caccaccatt gagggtcact tcgctgctga acacacccct tttggcaacc tcacacctga
      721 gcagatgcaa tttttgctta ccttcgttaa gtgcgagggg cgccttaacc gcatggaaga
      781 cgaattaaac ctctcttacc ccaccctgcg cagtcgcctc actgaaatcg tgcgcgcgct
      841 cggttacgaa ccaggcaaag atgaagggcc cgctcaacta tcaccagagg gtaggcggga
      901 tatcctggaa aagcttgacc gcggcgagat ctcttgggag gaagcacggg ccatattgcg
      961 cggcgagaag ttcatccctc agcacaacgc ggcagaaggg ggcaggtaat ggcaactggc
     1021 gaagaaagac tgaaagtatt aaaaatggtg caggagggaa agatcaccgc tgaagcgggc
     1081 gttgagctgc tcaaagccct ggacgccgcc agcaagaaag ctgaagttga cgaacgcgct
     1141 gcggtgaatt ttcctggcaa gggcggcgga aaatttttca gggtgcgtgt gaccgatact
     1201 gacaccgggc gcacccgggt gaacattcgc ctgcctttgg gcatggtcaa tgccggcttg
     1261 cgcatgggta tgcgcttttc tcccgaagtg gagggtctcg acgccgcgcg cctggttgag
     1321 gctttggcct ccggcgaaac cggaaagatc gtcgatgtgt tagatgatga agacggcgag
     1381 catgtcgagg tttatatcga gtaatctgta agcagcccgg gttcgccttg atcccgtttg
     1441 ttgcccggca gtagcctggc gcagcccgcg cggctgaagc gcctttagcg gtgggctggt
     1501 tccatcatca ctctgatggc ggtacttcct taagcactgc tgtcattctc cacttatttt
     1561 ccttggctat caattcgcct gacctggtca accccttcca ttcaggccgt tattggtttc
     1621 atactgccga ctggttggca aaactttaaa tacaaacggg acggcagctg gccgtcccgc
     1681 gcatccatct tctttaccgg ttacaggttg taaatttctg catatttcgc gcgcaggtag
     1741 cgcatatatg ggtctgggcg gatgcctgtg ccagtgacct ttttgatcaa ctgttgcggc
     1801 tcatacttgg caccatgccg gtggatatgg gtgcgcaacc aggcaagcag gtcatcaaac
     1861 ttgccccggc tgatctggtc ttcaagatct gggatgtttt ccatgatttt ttcccacaat
     1921 tgcgcggata ccaggttacc caaggcatag gtggggaagt acccgatcat cccgcctgac
     1981 cagtgcacat cctgcagcac gcctaggcga tcattgggcg gtgtaacacc caggtattct
     2041 tgcatctttt tattccacaa ccccggcaga tccgccaccc gcacgctgcc gtccatcagg
     2101 ccgatttcaa gttcaagccg cagcatgatg tgcaggttgt atgtggcttc atccgcttcg
     2161 acgcggataa gggaaggctc aactttgttg atgccacggt aatactgttc caggctgacg
     2221 cccttaagct gctgcgggaa tttttgcacc aacctgggat agaagaactt ccaaaaggct
     2281 ttcgagcgcc ccaccaggtt ttcccacatg cgcgactgcg attcatgcac ggccatggaa
     2341 gcgccgtctg ccagcggtaa tcgccgcaaa ctggcggaga acccctgttc gtacatggcg
     2401 tgaccagcct cgtgcatggt gctgaagagc gctgacgcgg cgcgcgacgg gtcaaaccgt
     2461 gtggtgatgc gcacatcgtt aatcccaaaa ctggtggtga atggatgaac cgatttatcc
     2521 tgccgcccgc ggttccaatc gtaaccaaag cgggtgatta cctccacgcc aaaatcccac
     2581 tgaccctgtt catcgtaagg gacgtgcatg aaggcatctt cgatctcggg tttggcggcg
     2641 atttctttca gcaacgccac ctgctgcggt cgcagcgcgt caaagatctg ttgcacctcc
     2701 gctgtcttca acccgggctc aaacccgtcc agcagcgggt cataaacgtg gtcgtaaggc
     2761 tggaaaaaag aagcgtatgc gcggcgcagg tcaaccactt tttcgagatg gggttggaag
     2821 atggtaaagt cgctctttgc tcgcgcctct tcccacgcca tttgcgccag cgtggtcacg
     2881 cgggtgaaat ccaccaccca ttccgtcggt actctggttt ctttatcata acggtgtgcc
     2941 acgcgctgga tcaggcaggc gtcatctgaa tccgcgtcca atgtttctgc gtagggtttc
     3001 agttctgcca gcagttctcc catctcgttg caagtgaact tctgatgcgc caggctggcg
     3061 atcgtttcca ggatatctcc gcggtcttct ccgccaccgc gcggcatata cacctgctgg
     3121 tcccacccca acacggcttc cgcgtgtccc aggtcggtga cttccatgat catgcgctta
     3181 aaccgctcta cctttttttg catcatctct cctgtcatgt tcaggcgact ttccagcgca
     3241 ggggtttgcc ggcaagcgcg ttgaggattt cttccgcgat atcgttggcc gcgcgcgcct
     3301 gggcttcaac agtctgtgcg ccaatatggg gtgaatcgat cacgtgcggg tgcgccacca
     3361 gctcggtcgc cccagggggt tcgttcgcga acacatccag cgcagcgccc gcaacgtgac
     3421 ctgagtttaa agcagccagc agcgcttctt catcaataac cccgccgcgc gcggcgcaca
     3481 caatgtaaac gcccgccttg gttttggcaa gggcttcagc gttcagtaaa ttgcgtgaat
     3541 cagctgtgag aggcatgtgc atggtgatca tatcagcagc agtgagcaat tcatccaggc
     3601 tcaccggttc accaccgcgc ttcttgattt cttcggctga aaccagcggg tcataagcca
     3661 ggattttcat ttcgaaggct ttggcgcgcg ctgccacggc tgagccaatg cgcccgaaac
     3721 caatcacacc gagggttttg ccaaatagct cacgcccttc gaattctttt ttcaaccatt
     3781 tacccgcttt cattgaagca tcagcgcgtg gaagttcacg gacaaggctc aacatcaggc
     3841 tcagggtcag ctcggcaact gccacggtag tggccaacgg cgaattcaca acggtcacct
     3901 ggtacgcttt cgcggcagcc aggtcgatgt tatccacccc aacgcctgcc ctgcccacca
     3961 cttttaaatg gggcgcggca gccaacaccg actccgtcac cttggtgcga ccgcgaacga
     4021 tcagggcgtc atactgcccc acgaccttta agaggtcctc cgcgctgatc cctgattggt
     4081 ctgttacctc tgccgcagac cgcaagatct cttttccgtt ctcttccaat ccatccgtca
     4141 acagcacctt ccaggcagcc attcttactc cttgtttttg tatagactgt tgggggaaaa
     4201 cccccatcca ttttacctta tttcattaac agcgcctttt tttgatgaga atttcgcgca
     4261 ttttggaggt ggtacaatct gctcatggac gctaccacga aaaaagcgct cagccgcgtt
     4321 aacccgcggt tgcttcaatc ggaagccatt cagcgctgca gccttgcaga ttgcaaaggc
     4381 acctgctgcg tctttggcgt gtgggtagac ctgcgcgagg tcgatgatat tatgcgcaac
     4441 gctgccatca tcctgccgca tatgccgccg gactgccgcg tacccggcga gtggtttgcc
     4501 gcggtggaag acagagataa gcgcagccca agcagtcggg tcatccatac agcggtagaa
     4561 aaccgggctg agcattacca gggaaccgcc tgcgttttct gcctcgcgga tggcaaatgc
     4621 gcgctgcagg tggcggcgct ggcaaataac ctgcatccct ggcgttttaa accattttat
     4681 tgtgtgctgc accccctcga cctggatgaa gaaggcagga tcacgttaga cgccgcagaa
     4741 gatatggtga acgagcaggg aagttgcctg agacgcgcgc ccaaacccat cccgttactc
     4801 gataccttcg agccggagct gaaatatctg ctgggtgaac agactttttc ctgccttaag
     4861 cagttcactt cacgaaacac cgaagatgag ccggagtaag accgccaagc tttgccatgg
     4921 gtgggggaaa aaccgtgata ggttgaacag atctttttaa gataaaatat atctgaatat
     4981 gatgattcga ggaattgtgc attgaaacca agactgagcc ttgccgtgtt gatcctcacc
     5041 gccgttttcg ctgctttgtt tgcgcgggta cagggggttt ccgcgctcac ttcccccggt
     5101 aaaagtgaag ctgatagcgc cctgtgcctg cccgagacaa gcgaagaact gatgaacggc
     5161 tgcctgaaag ccggcccggc cgcgcgcctt gaagaactgg ccgccagggg aatccccccg
     5221 cagggacggc agctgttcgc ctcgcggacg ccgtatgaac tcggcgccac acccttctct
     5281 tacgccaaac tggtcgatga ccaggaaatt cccctgtacg ccacgctcgc tgacgtcgaa
     5341 agtggtaagc ccagcaacgt cctgccaaaa agccgtacca aatacgtggc gttaatgaac
     5401 agcgctgaga cctcgcgtgg tcgtttctat cagatcgcca ctaccgaatg ggtgagcgtg
     5461 gagctcgcca agaaagtcgc ggcgaccaac ttccagggat acattcttaa agaaagtccg
     5521 gatttttcct ttggctggat catcaatgaa acgaccagcc gcgctggtcc tggttacgaa
     5581 taccccgaaa ccggtgtgaa ttactggcgg ctggaccccg ttatggttta tgactctgca
     5641 gtggtcaacg agaccgagtg ggtgatgatc ggctataacg agtgggtgga acaccgggtt
     5701 atatcgcgcg tcattcccaa ctatcagcgc cccgcaggcg tgccagttga caagtggatt
     5761 gagatcaacc tgtatgaaaa cacgctgacc gcatatgaaa atggtcagat ggtttttgcc
     5821 accctcatcg cctcaggagt caaccctttc ttcacccagc ctggggtgtt ccagatttac
     5881 aaaaaactgg aatccgaccc catgagcggt tctttcgaag tcgaccgctc cgattactac
     5941 tacctggaag aagtacctta catcatgtat tatgacaaag cccgcgcgat ccacggtgct
     6001 tactggcagc cgttcctcgg ctaccagaac tcgcatggat gcgtcaatct cagcgtggca
     6061 gacgcgcact ggatgttcaa ctgggcgaac gatggcgact atgtgtacgt gtgggatcca
     6121 tcagggaaaa tcccgaccga ccccagcttc tacggagacg gtggatttta gggctttctt
     6181 ctttagtgat gactcatttc ttcaaagaac agtcagccga cgttcaacct cataaagtcc
     6241 tatcttgaac acaggaagga taaaatgaaa ataaaaaaat ggatttttat cgccgcgacc
     6301 accgccctga tgagtgtagc cggcagcgcc tgcgcaccag ctgcagcaat cgaaagcact
     6361 cctcaaccaa cacaaacccc ttatgtgatc gtggtaactc ctacagcagc ggaggtttct
     6421 gccacacctg agcctaccga accccttgca gcgcccgatg cggaaacacc ccggggtgaa
     6481 gaaccgccca agtacttccg tgaagaattc gatggaggtc ttgacgattt tgaacagata
     6541 ttatggacag ggcgtttcgg taaagataag atttacaccg aagatggaaa attaaaggtg
     6601 cgcctcaacg cgatgtacct tgcgccttac ctcacctatg acccgtacac ttacacggat
     6661 gtgcgcctgg aagtaagcgc tgaaaacctg gcatataacg acaaccacat caccctggtg
     6721 tgccgctacg atgaagaatt aggctggtac gagttcaata tcagcagcaa tgggctcttc
     6781 cgtatcaatt actacgacaa cacgctcgtt aaaggtcacg tgaagttatg gaacggcggt
     6841 tccaacgaca ttaaaatggg caggcagatc aacaagatca ccgcagagtg cattggtact
     6901 caactctcgt tgtatgtaaa cgatgtgctc ctcaaaaccg tagagcacaa agacctgaga
     6961 gaagggcgcg tagggctggg cgtatcctct tttaaccaat tcccggtgaa cgtggacttt
     7021 gagtatttcg atatcagtca accgtaattg tccagcacct cagaagacct gacatgccaa
     7081 tgcatggtgc ggatggggct tgaaataaaa gaaatctcgt gtactatata agtaccatcc
     7141 aaaattagat aagtaagaca acaaggagaa agcgatgaat aaaaaagcgt tcatctcatt
     7201 gatgatgatt gccctggctt tgaccttgct cgtctccgcc tgtggcaatt ccaaaggagc
     7261 agccacaacc gaaccccctc aggtcatcat catcactgcc acacccgaat ctcctcaggt
     7321 cattataatc actgccacat ccgaggaaat ggccccgccg atagtggcaa ctgaacctcc
     7381 cgccgccccg ccaacggagc caccagatgc ggcaactaga ccaacgccgc catttggata
     7441 cgaaccaacg ccgttcatag cagaagacag cgcgcaagga gaacggtact tcctggatga
     7501 atttgataat gggttggacc agtacgacca cttcatttac gacatcactg taaaagtaaa
     7561 taaaattatt aaagaaaacg aagaacttga aaagaaaatc aaagtcgagc ttgaagacgg
     7621 tactattgaa ttcaatatcg agcggtacta tctcaactac tatatgattt atcgacccca
     7681 ggtttatgag gatgtgaaaa tctctgttga ggtcgaaaac ctgggtttcc ctgccagcca
     7741 agcagcattg gtctgccggt atgatgagga acttggctgg tatgaactca ttgtgagcaa
     7801 cacagggaga tggaggctgt attattttga taaacccctg gtgaaatgga acttcttatc
     7861 ggatggcggc gctgaaaagt tccactacgg caaagacacc aataaataca cccttgaatg
     7921 caagggcaaa gagctctcag tttacatcaa tgatgtcttt tctcaaaccg tgaccaataa
     7981 aaacctcaag aagggcaagg tgggtttttc aatccaggca cccaagtctt ggaccaccat
     8041 gaacgttccc tggtttgagg tcagcgaacc ataagaccag cgtatcaacc aaaaaagcgg
     8101 ggagtccaac tccccgcttt ttgttgcaaa tgttcccgct tctcagtctc cggtaatgaa
     8161 cctgctaatc accatagcgc taaacaggcc cgcggaaagc aggatccccc acagtaagcc
     8221 gccgctcaac ctggaatctt ggagttcgta accggtggac gtttgttgta ccagcagcag
     8281 cggcccagcc atccaggcaa acaacgcccc tccagccagc ccgcccaggt gcccccagtt
     8341 atcaatccct ggttgcaatc cgatgagcag attcacaacc acgatcagca ccaggtttga
     8401 aagcatggcg cgcgcccgcg aaccgaacaa cctcctgttg cggtaaataa aaacgccttc
     8461 ggcagccacc agcccgaaca ccgccgttga agcgcccagc gaagggtttg gcgagagcag
     8521 gaaagagagc gaattgccgc aaaaagaccc gatgatatag agcaccagaa acctgccatg
     8581 gccgtagtaa cgctccaatg aactgcccag cgagaacaag gcgtacatat taaaagcgat
     8641 gtgcagcagg ctgccgtgca gcagcgcggg ggtcaccagc cgccacagct cgccgcgcag
     8701 gatataggca ttgatcttcc cgccgagcag gaaaggccaa tcaacgccgt cgtggcttgt
     8761 ggattgagag agcaactgta acaggaacac cgcaatcgtg atgccaatga gcccataggt
     8821 cacccatggt tttctaaccg gcagccttac tgtaacaggc gtccccaccg gcggtggctg
     8881 aggctgctgt tcttcctgag agcccgcgaa gcgttccatc agaggatacg gcggtgtttc
     8941 accgccatat gttcggtttt aataatggaa accacgtcat ccaccgcctt ttcgatctgt
     9001 ccatccgggt tcaccaccac ataatcgaac aacccgatct gttttacttc ttcacgggct
     9061 ttcgccacgc gcagccgcag gtcatctgct gactcagtgc cgcgccgcgt tagccgttgg
     9121 taccattcat caaaagaccc aggaacgata aaaatcagca cggcctgcgg atactgcttg
     9181 cgcaaggttc tggcgccttg cacgtccacg cgtgccacca catccttgcc gctcgccaac
     9241 gcttcttcta tttgatggcg cggaatcccc ttaagatcag aatataccac ggcgtgctcg
     9301 gcgaaccagc cttcggcttg ccgggtttta aattcatctg gggtgaagaa aaaatagtcc
     9361 ttcccatcca cctctcctgc gcgtggcgcg cgtgtggtgg cagtgatcac cacatgcagc
     9421 ggtaagtcgc gctgcttcag ccctctgatc acgctgtctt taccgacccc tgaagtgccg
     9481 gatataacga tgaacaaggg ctgcgggtgg attaggtcaa aagacacctc ttcagaagac
     9541 atgctgaacc tctcagggga atttttacac agttctgata tgctgtgatt ataatggata
     9601 atgtcagtaa tcaactgagg tagctatgcc gatctatgat tttcgttgtt ccagttgcaa
     9661 gcagcgtttt gaagtgttcc tcacctacgc ggaatatgac caccatcaga ccgcctgccc
     9721 cagttgcggt tccactgacc tgcaacgtgt gatccgtaag gtgcgcgtcg cgcgcggaga
     9781 tgcaggacgc ctggagtcgt tggccaacga cagcgacctg gatatgattg accatgatcc
     9841 caaagcgctg gggcgcatga tgcgggagat gaaaaacgag gtaggggcag atgaccttcc
     9901 gggtgagttc gatgaggtgg tcagccggtt ggaaaaaggg caaacccccg aggagatcga
     9961 gcgcgattta ccagaactgg gggaagaatg atcacgcctt tcagtctttc cctgcgaggc
    10021 acgtagcgct cagtgcgcga gcgctttctg caggtcagta aaagagttca tccaggcgat
    10081 gcggcgcaaa aagagcgcca gtgaacccgc ctgctctctc aacgcgcgca ctgccggacc
    10141 taccgggtct tctccggctg ccccgccagg catatccgca taagcgccat gaaaggcaaa
    10201 gtaagcctgg ttcagtttgc ggatcatata gccgtgctcc acgaagacca cgcggcgttg
    10261 ctccatatat tcttcggctt cttccacctt gccctgcgcc agtaattcat ccacccttac
    10321 gcgggttttg tgcatctcct gacgaaaatc aaactcgttc acggcagttc tttttaacca
    10381 cgctttcggc gttttgtttt cgcccgtgtc atcatcagca tagtagcgcc gcatcacttc
    10441 gcggctgatc tccttcccaa caatcgaagc agcggtctcg ttcatggtgc gcagctcagc
    10501 cgtggtgtca tagttccaac ccaggggatg cagggtcagg taattatgcg tccactcgtg
    10561 ggcgatggtg tcaagcgtcc attcgatatt cgaagaacgc tgcaccatgg tggggtatac
    10621 gccaatcccg cctacatcca ccaccagcgc cgaagcattc atataagacg acacattttc
    10681 ttcaagcctg gtgatgtctt ccaatcccag gtcagccagc agggagatgt tgacttcctg
    10741 gcgaatcgaa tcgcgcggag atacgatcag ggcttttggc agcggcgaag aatgaaaaag
    10801 caccgggggc agcggctgcg ccagaaaacc aatcccctcg tctgccatca ccctggcaac
    10861 ctggaattgc aagatggctt ccaccaatgg ggatagacca tccagcgact gttgcagcgc
    10921 gtgctgctgc ctgagcagtt cagcggcggc tgccgcggca tcactgatgg cagggtctgc
    10981 gtataccagt tccaccttcg cctgcacaat ttccaacgcc tgcacaagcg caaagtagcg
    11041 cttcaccaac cgggtttgct gttcctgcgg caatctttga acggggttta acagcgcctg
    11101 agatgccttt tgccctgccg cgtttgccaa ccaggcgata taatcatatt cgatatcggc
    11161 agtgaacagc cgcgcctggt ccagcggatc cgctaaagaa acaaccgagc cgcataacaa
    11221 caccatcatc atcaatagcg ggaccgcaat cccggtatat ttacgccagg tcatgcgcaa
    11281 attatacctg ttcacgctga gcgcattatc attaagagaa ggcattaagt tcctatgaaa
    11341 aaaagtttga caaaaaaaac catcctgttc atcctcctcg gtctcgcggc tgccggtgtg
    11401 attgcctacc tggtgctcaa taccctcagc gcccgcaaag ctgccgtcgc cgaactccag
    11461 acagttgaag tgaccagggg tgaactggtt gccattatcg gagccaccgg cacggttcgc
    11521 gccaaccaga gcgcggtgct cgtctggcaa accagcggcc gggttggtac cattcacgtg
    11581 aagacaggtg aaaaagttgc tggcgagacc atcctggcag aacttatcga atcctcactg
    11641 ccgcaaaata tcattttggc ccaggcagac ctggtggaag cccaacgcgc actcgataac
    11701 ctgctggatt ccaacctgag ccaggcgcgg gcgcaattaa acctggccaa cgccaaagac
    11761 gcttacgaac gcgccgtatg gtcaagctgg catggagata ctgcccgtac caccaaccag
    11821 aataagatcg acgcagccca ggctgcagtg accctggctc gcgaccgggt gaacaccgct
    11881 gaggaaaact acgacaaagt ggatgaacgc gatgatgacg acccgctgaa ggccaacgcg
    11941 ctcagcacgc tggccaacgc gcgtgaaagc ctggataaag ccatcaaaga cctcgattac
    12001 ctggtgcaga accccaatga aaaagaaatc gccatatcag agggcaaggt tgcggtagcg
    12061 ctggcggaat atgaagacgc ccaacgcgaa tgggaacgcc tgaaggacgg ggcggacccc
    12121 aacgatatcg ccgcggccaa ggctcgtatc gcggcgatta aggccaccat ggcgctggcg
    12181 catatttccg ccccctttgg cggcacggtc accgaggtca atatcatgcc cggtgacctg
    12241 gttaccagcg gaggggtggc tttcagactg gatgacctct ctcatctgct ggtggacgtg
    12301 caggttcccg aggtggacat caaccgtgtc gtggtcggtc agcctgttga gctgaccttc
    12361 gacgccatta acaaccaggc ttaccagggc aaggtgaacc gtgtggcccg ggtaggcacg
    12421 attgaatccg gactggtcaa ttttaaggtc acggtcgaga tactcaaccc tgatgaacag
    12481 gtgctgccgg ggatgaccgc tggggtgaac atcatcgtca gccagctgga ggatgtattg
    12541 accgtcccta accgcgcaat ccgcctggta aataaccatc cagtggtcta catcctgaag
    12601 aacaacatcc ccactgaagt gagcatcacc attggcgcct cttcagattc gatgagcgaa
    12661 atcctctcag gggaccttaa agaaggggat aaggtcattc tcaacccgcc ttcctccttt
    12721 atggatgtgc atttcagcgg aagaccgcct ttctaaggca cctttatgga acccgtcatt
    12781 caaacgcatg aactgaccaa aatttaccag cttggcgaag tgtccgtgca cgccctcgcg
    12841 ggggtcagta tcaccatcga ccgcggcgag atcgtcgcca ttatgggtcc ctcgggctca
    12901 ggcaagtcca cgttgatgaa cctgctgggc tgccttgacc gacccagcag cggcacttac
    12961 acgctggatg gagaagaggt tgcccaactg gatgacgacc atctggcaaa catccgcaac
    13021 cgcaaagtag ggtttgtctt tcagagcttt aacctgctgc cccggcaaag cgcgatcgaa
    13081 aatgtagagc tgcccatgcg ttacgcgggg gtgacaaaag agaagcgcga gcgggcgatc
    13141 gccgcgcttg aatcggttgg cttggctgac cgtatgcacc accgcccgcc cgaactctcc
    13201 ggcgggcagc agcaacgcgt ggccatcgcc agggcgctgg tgaacaaccc cgccatcttg
    13261 atggctgacg agcctaccgg caaccttgat tctcgttccg gtcaggagat catggaattg
    13321 ctgttgaaat tgaaccagaa ttcaggcaca acgttgatca tcgtcacgca cgatcagggc
    13381 gtggcttcca acactcagcg tgtcatccgc ttaaaagatg gcctggtaga gggagatacg
    13441 ccatgaacct ctggcaggcg atcgtggagg cgcttgaaag cctgtattcc aacaagctgc
    13501 gctcatttct gaccatactg ggaattgtca ttggcgtcgg cgcggtcatc gctatgatgg
    13561 cagttggcgc tggcgcgcag gaaaccatca ccggttccat cagcggcatc ggcagcaacc
    13621 tgctattcgt gattcgcggt aatcctactg tagagctcac ccgcatcgag cccctcacca
    13681 gtgaagacgc agaagccttc cttgaccagt tccaggcgcc atccgtagcg ggtgtggcgc
    13741 ctgtggtaaa tgggtcactc gtggtcagca cggctggtga atccacccga acgaccgttt
    13801 acggggtgac ccctgcctat caacagataa gaaattacgg gttaacagaa ggcgttttta
    13861 tttctgaaga acagctgctg ggcaggtctt ctgtggcgct catcggtcca gacaccgctg
    13921 aaaaactctt cgggcggcgt gaagggttgg tcggcgaaac cctgcgcatc gaagggcagc
    13981 ccttccgcgt gataggttta ttagagacga aaggcggctc atctttcggc tcacaagatg
    14041 acctgctcat catccctctc agcaccgcgc aggcgcgcct catccgccac cctgataacc
    14101 gcctggatat gatcctcgtg caggttatct cgcccgaact gaccacgcag gcaactgaag
    14161 agatcgctca aatcctgcgc agccgccacc gtacgcgtat tgggcaggat gacttcacca
    14221 ttttttcgca agatgatttt gtagacctgg ccaagaccat caccggtgtg ctcactatct
    14281 tcttgggtgg tattgccggc atctcgctgc tagtcggcgg catcggcatt atgaatataa
    14341 tgctggtttc ggtaacagaa cgcacccgtg aaattgggct gcgcaaagcc ctcggcgcgc
    14401 gtaaaaagga tatcctgatc cagttcctca ctgaatcctc gttgttgagc ctttttggcg
    14461 gcattatcgg catcgggctc gggtggctga tttcttatgt ggtcgggcgt atcgcggtgg
    14521 ccaacaacac ccctttcacc ccccaggtca gcctggatgc catcctgctg gcgacgcttt
    14581 tctccaccgc ggtgggatta ttcttcggtc tttatccggc aaaccgcgcg gccaacctgg
    14641 agccggtaga agccctgcgc cacgaataac gccgcagact gagccagcga acgaagcctg
    14701 gatcggtaac agcctggttc ttttcttctg gctgggctaa cagtttataa aggctcttca
    14761 gggatcacga tcaacaggat gaggtacgcc agcagcgtgc cgcccacaaa gagtaaaccc
    14821 agcacaaaca acagccgcac aagcgtgggg tcaataccaa aatattcgcc cagcccgccg
    14881 cagaccccgc caatcttgcg gtcaaccctc gagcggtaaa acttttttgt gttgttatcc
    14941 atattcatct cctgtgatat ccttcatgtt gtttacgctt caccggttag aaagttgcag
    15001 cgctgaagag agggttgaat cgtgtacaat agaatagccc ggtgaagatg cccgctgaag
    15061 gaggaaatat ggctgaaaaa acacaggctg agaaacatca gatcattcat ctacttcaac
    15121 aaattccgtt cgacgagact gaaaaagctt tatggatgga aaacgtggaa gaaaatggcg
    15181 ttacagagga acttctggac gaaatgcaca caaagctgtt ggcgctcccg caggaaaagt
    15241 ttgccagcga atgggtgaaa gccaaattat cgaccgatct cgccagggtc gcccgtcaat
    15301 ggcgcatgca gcgcgccagc cggcaattca agcacaaccg ctaaagaacc tgccctgcca
    15361 cccgggcgca aaatcccggg tggctttttc attttttggg tacaatcaac aaatcgatga
    15421 accaagacac tgaaaaaatc aatttgcgcg aaaggctggt atcagatacc tatctactgc
    15481 tggccgccat catctggggt gctggttttg tcgcccagcg tatggcttca tttcacctgg
    15541 gctttaacgc gtttaatggt attcgattct tgctcggcgg gttggtgctg ctgttatttg
    15601 tttggcggcg ttttcgcgcc ttcaaaggtg gttggggttg ggtattgctt gccggaggcg
    15661 tgctgttttc tgccagctcg ctgcaacaga ttggcattgg caccacttct gccagcactg
    15721 ccggcttcat caccgggacc tatgtggtgc tcgttcctgt cctgctggcg ttgttctggc
    15781 gtcaacgcac gccagtggtc acctgggttg ctgcgctggt agcgctcgct ggcacctacc
    15841 tgttaagcac tggcgggact gtgctgatcc cctccacggg cagcctcatc ctgctggctg
    15901 gctcggtggt atgggcgctg cacgtgatcg tggtggggct ggcagtgaaa aaaatcgacg
    15961 tgcttgtctt ttctgtgggt cagtttttgg tttgcggttt gatccacctg gtaatgagca
    16021 tgttcattga accggtgagc atgcccgcac tgcgcgccag ctggctgcca ttgctatact
    16081 catctttgtt ctcggtcgtg ctgggtttca ccttgcaggc catcgggcaa aagaaagccc
    16141 cttcagcgga tgccgcgctc ttattgagcc tggaagcggt gttcgccgcc atcacgggtg
    16201 tgcttttctt gaacgaacaa ctaaacctgg ttcaggtgat tggatgcgtg gtcatcctgg
    16261 gcgcgatcct ttttgcacaa ctgatcgtgc tcaaacaaaa tcgaaagcct ttgtgatggg
    16321 cttgaacgat tttcttgaca tacgccagtg gctgtgtcaa aataggaacc agcgggaaat
    16381 atcagcaacg aaaaagaggg acgcttttgg agttaattga ccggcaagcc ctgatcgatc
    16441 gtatacgcgt tctgcagcac agtttgccag aagtgcaggc cgcggctgtg atcagcctgg
    16501 atggcttgat catggcatcc gctctgccag aaggaatgag cgaagaccgc atctctgcca
    16561 tgtctgctgc catgctttct ctcggagagc aaatcaataa agagacgggg ctgggcacgc
    16621 ttgagcagct ttacaccagg ggaagcaacg gttacgtggt tttactagca gtggggaaag
    16681 aagccgtttt taccacgctg gtgcagccgg aagccaagct gggcatcctt tttttggagc
    16741 tgcgcaaaac cgccgaagac ctggcgcgcc tgcttaatgg aaacggccta aacacgtaag
    16801 cccggtaaaa gaagcattca cgcctgattt tccctgcccc aatcttccct tcaactggta
    16861 gaattacgct gtttgcccac cttggcgtta attcaggaga atccttgagc atctgcgtca
    16921 tcatcccgac ttataatgag gttgaaaatt taccgctcat cgtcgcacgc ctgcgcgccc
    16981 tgccgctgga ggatctttcc atcctggtga tcgatgacaa cagccctgat ggtaccggcg
    17041 cgctggcaga tgaactggca gagagataca aagggcaaat ctcagtgatg caccgcaaag
    17101 gcaaacttgg cctgggcacc gcttatatct ccgggttcaa gcaggttttg caaacaaaca
    17161 ccgcgtacat cgcccacttg gacgctgatt tttctcacca gccagaaaaa ctgctggagt
    17221 ttatgaaatc catcgagggt agcgatgtgg ttttcggctc aaggtacacc ccgggcggca
    17281 gcctggaccg ccactggccg gcctggcgaa aatggctctc caacttcggt aatacttacg
    17341 cgcgcaccat tctgcgtata cccaccaaag acctcaccgg agggttcagg ttgtggcggc
    17401 gagagacact ggcggcgatg ccatgggata gggtgcggtc caatggttac gttttcatgg
    17461 tcgaaaccgc ctacctggcg cacagacttg ggtttaaaat caaagaagtg cctatttatt
    17521 tcgctgaacg tactcgcgga aaatccaaga tgaatttttc aattcaggtg gaagccgcct
    17581 tgcgggtgtg gagcttgatc tctcgttacc gtgatatcca gcccctgccg cggggttaag
    17641 caggcggggt ttcctgctct cttttcgctc ccagcaggtc tttcaactcg ttcaccagcg
    17701 tcttccattc caccaggctg agcgtttgcg cgcgccgctg cgggtcaata tccgcgtcta
    17761 ccaggtatcg cgcggcttct tcgccgctaa gatgtaagcc agccgccagg ttattccgca
    17821 gcgttttgcg cttttgagca aaccccatgt ggataagaga gaaaaacatc ttccgctgtt
    17881 catctgtaaa gtatggctgc gggtgcaggt ccacccgcag cgtggcagag tcaaccccgg
    17941 gaaccgggta gaaagccccc gccgggatga cagcgctgat gcttggttga ccaaaaacct
    18001 gcacgcttaa cgccagcagc gagaggtctc cgggttctgc gcaaatgcgc tcagccacct
    18061 ctttttgcat ggtgagcacc agccgcgccg ggcgctttgg gcattccagc aagtggcgca
    18121 caatggcgga agtgatgtaa taggggatgt tcgcgacggc gatatacccg ggttcattca
    18181 tcagcctggc ggggtcgagc gccagaatat ccccctgcac cagctcgaca tttgcgtaac
    18241 tggaaaggac ctcgcgtaaa acaggcatca acctgtggtc gatttctacc gcccgcacca
    18301 ccccggccgc ggcggccagc aggcgggtga ggctgcccaa cccggcaccg atttccagca
    18361 cttcttctcc accagtcagt tcagcgcttt ttactatcct gcgcagcgcg tgatggtcga
    18421 ccagaaaatt ctggcccaaa cctttacgcg ggtcaagatg atggcgtttc agcagtgagt
    18481 gaatatccag cggcgcgggt tctatggcag caccccggtg aaccctgacg ggggtggaga
    18541 gagaaagtag attttaacct tcaccgctcc ccagggaaca taatccgcgt cactgtaacc
    18601 caggtcgatc cagctatcgc tgtacgggta agaccccgtg tctcccacaa ccccaatgcc
    18661 ataaccaggc acgtagaccc tggtatcgcg aaacaggtta aaccagttgc gtttgaacgc
    18721 cacaacgcct ttttgaatgg tgcgccccga agcggtgccg tttgagcaat atccttcaat
    18781 gccttgccgg cacggagaaa aagaagtggc aacagcatca atgaccaaat aatactgaag
    18841 cgctccttct ggtgtgtcga tgtaattttc aagcatcttc gttccacggt actccacctg
    18901 ggctaccggt gggataagga tcacctcgtt ttgggtctgc cgcgaaacct ccagaccgtc
    18961 ttcatagcgc actttcacaa gcgtagccgc caacccgttc tgcccggcct gtgtgatgtt
    19021 ggtttcatta atgaacattg tgtcatcagc caacgactca atttcaaagg ggatcacgcg
    19081 ctgttcggcg tgaatttctt ctgtcacccg ggtgacattg atgacgccat cctctgggag
    19141 cggatcacca gcgcctggct ggctggtatc gatatcctgc agggtcaccc cgttttctgc
    19201 cagggctgcg gcaaccgtgg gcgcggcgct gtatccttcc agtatcgtcc catccacaga
    19261 aatggaaagc gggcgggcgg tgtgaatttc aactgcgagg ttctcggtaa gcgccgtatc
    19321 catccctgga gtgacccggt ctccttcacg cagtctgaat cccgcctgcc acagcgcttc
    19381 tccgagagtg cccgcgctgg aagtgatggt cgtgtctctg ccatccacat tcagcctgac
    19441 ctgtaccgcc gggcgatagt tcagtgtcat ttcgcgcccg aaggggagtt tttcgtttgg
    19501 cccaaccggc aacccattga gcgtgaggct atcaaggctg ccgagcgtga gcccggcctg
    19561 tttgaagacc tcagcggcag tcagcgcgga agtagacagc tcaatgggtt gtttttgggt
    19621 tgtgtcataa acccgcacca acctggcgtg gtcaaggcgg atctcatccg cctttgaaag
    19681 ccaggtgcca gcagcaggga acaccgcatc cccctcatcc acggcgaaac ccgctgagcg
    19741 cagcgcgccg ccaactgtga gcgcgtgtgt gcggatggtc agcggcgtgc catcaatggt
    19801 cgccggcaca ttgcgcgtca tcaacaggta accgaccaca aggccaataa tcggaacagc
    19861 gatcatcagc atgggccagt gtttgcgttt catgccaaga attctatcag attaaaccgg
    19921 ctacctcaca tcagatgatt tcctaaaaac ctcccggttt tccggttact tggcgatcct
    19981 tgggaacacc caatgtgccc aatcctgtga ggaagaccca ttagcaaaca caataaactg
    20041 gaatttcaca tcgctgcccg cgagagagga tagatcaata tcaatgtcta agggtgcgtt
    20101 ggaatacttc atgctccagg ttcccaattc aacaaacgga tcagaaccct ttttataatt
    20161 caccctgaaa agaacatcac aacttgtacc gctgtaaccg caacctatag tggttttaaa
    20221 ccgattccca ctttcgatcg acacagccgg gaaaatccca acaatcgccc caccatccac
    20281 ccactctggg tgagtgagaa gtgactttgt atcacgcgta ttgccatcct gtaatcttgg
    20341 tttatcctgt tggacaacga atcccgttga gtcgttctta gtgcccgggc aaggaagatc
    20401 ctgatgtcca ttactccagg ttgcgctgca gtaattcgcc gtgaaatcat acttcggaaa
    20461 aacaataggt atcaatgtaa aatgcggtaa agccgtcagc aggattagcg gttcaacctt
    20521 gattaccgcg gtcatcgggt aggcgtaacc agcgccaaaa ccgaagatgc ggccggtgtc
    20581 actgcgcagc atgaatttcc aggtgtaatc cccgttcgtc gtgggcgcgg tgatgaccac
    20641 cgaaacatcg atcgtcccac caggcggcac attggctggg agaggcgtga cggttggcgc
    20701 gccgctactg tgcccgctat cgaacaccac ggcgtaggct gtgttccagg agcaggtgcc
    20761 ggtgttctga atacgccagg tcttggtaaa ggccaaacct ggagtaatct tggtgccatc
    20821 ggggatcgtc acatcctgta cgaacttggc ctggttacag gtatcagtag tgggactggg
    20881 gggcagtgta gggattgatg gcgccagcgt gggtaacggc ggcggtgttg ttgtgggctg
    20941 ctcgaccggg ggaggggcgg gattggttgg ttgtaccgct ccgtcaggca agggcagtcc
    21001 acctgtgggc tgcgcggcag ccactgtttg cgccacggag gtgttgacct tgtcactcgc
    21061 gctgattttg gcagtgttgg tgcaagaaat cagcaaacag gcgaccagaa aaatgagcat
    21121 tccttgttta atccgggtga tcatttcaag ctccttttga atcagatttc tctgttttga
    21181 ttatacggga ttatccccta tattcatcac tgcaatttac tcatccagaa aacccgtttt
    21241 caggttttca gggtgtggtc acctgtcaat ccaaaagaaa atgttaccga aggtgcatcg
    21301 ttggttcaaa tgataa
//
LOCUS       DAOSPM010000025        20224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2655_length_20224_cov_13.450840, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000025 DAOSPM010000000
VERSION     DAOSPM010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 20224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20224
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2655_length_20224_cov_13.450840"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLW25_03755"
     CDS             <1..820
                     /locus_tag="PLW25_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53637.1"
                     /translation="DNHRNGYTRYTELSKPAAQSIGVGSTIITGTLFMLKTSFHKWRL
                     LLVSLLALANSSPMFYSLWSYSGGEIHRLTGTETKLKVTSGGMQATSTNSGELCVLFQ
                     EDHVKVDGCDKDSPITPWQSPPEWEVREVIVSDLNWDNQEEIAMLVWRPFKPWPVDRF
                     MPSAGRINTFHDKNDMSCHLILIGLRNGNIKELWAGSAMADPLREIHAADLDGDGRQE
                     LVALEHAYNGNPRASSITVWAWHGFGFSLQARQNGRFSSLLVLPAEQGKVILTQ"
     gene            840..3077
                     /locus_tag="PLW25_03760"
     CDS             840..3077
                     /locus_tag="PLW25_03760"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOS53638.1"
                     /translation="MNRLFKVLSVLLIVAFLLSACQLPFNLVERIKNALIEKGQSGEV
                     TEPAAQGTETTTTQPPVVYPAEGMAPVFAAYQQRPYTMPEVYQGGYTLPLKASELVNL
                     DLFDLTDSQVEALLNNGFVVQPPNNDPSRMYNEFYQAYESIRYEETPVFVTTDAIFHV
                     YHLIFDKMLRDLERTFFIDTLKELTTAMVTESVAQYEKLQGSPLEEAAIRNVAFFTVP
                     AMILDLPVSVPAEAKKLAEEELALINEGGGISASPIWKTEGQATDNVLLEDYSQYIPR
                     GHYTRDEALKTYFKAMMWFGRMTFRLMDPIETQRALLVVQAMRNASTTSGKPALELWQ
                     NIYDPTVFIVGKADDLSIYEYGKLSDEVFGVHPELTSFADPLLAEKFTQAVKQLPPPQ
                     INSMWVYIWQERDAVTQGFRFMGQRFTLDQYVFGKLIWRKVGTTENPRDLPKSLDFLA
                     AQGSEEAYNLLAEMGETKFENYDSQLAKVKEEVAGFGIDSWTQNLYWSWLYALQPIFA
                     PKGEQYPPFMRTQAWLRKDMQTALSSWTELKHDTILYAKQVMAEMGGGALDKPPRGYV
                     EPNLEAYARLLALTEMTRSGLENREILDDVTRGNLDNLIEELVFLLDVSQAELNGVKL
                     TEEQYWRIQYFGGWLESMTIAASDPADVALGGGNLSDQKSALVADVATGMGRVLEEGV
                     GYPTKIYVVHPEAPYEIAVGAVYTYYEFDVPLAERLTDEAWQKMLETGKAPAAPEWTG
                     MFIVK"
     gene            3077..4081
                     /locus_tag="PLW25_03765"
     CDS             3077..4081
                     /locus_tag="PLW25_03765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOS53639.1"
                     /translation="MKLRSSVQGALILALLVLTGCHRASDSSSLFLGGDVLLYRAGKP
                     LFPVEDEAISPWGDFIKVKQEEHADLFAVNLESPFGLRVVEHGLENPSMNLCADDDAL
                     QHLQQAGVNLITNHNNHVNDCGFSGQQYTDELLDRAGILHQRNHSSVQFVPAGGAVIA
                     VLSINDYERDYEIVTVVNELKKARGASDLVLVSVHWGQEYQAGPSQHQQELAQLLVDA
                     GADVIWGHHPHVLQRMEWLRSSADGHTGLVMYSLGNALSDQWMLPDAQRTALIRLTFS
                     HHQVKDILVVPLKMDMSSRTLLLEKDPQLFNWYVERLSLESLEKKGTTIRLFEFAQTP
                     "
     gene            complement(4085..4624)
                     /locus_tag="PLW25_03770"
     CDS             complement(4085..4624)
                     /locus_tag="PLW25_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS53640.1"
                     /translation="MTDLSTDHPSRAPLDEILVRELKKKDLPALEWDGEYTHFRNVYR
                     GVYQRTRLGTAAAWVAESPVDGIVGQVFLQLDCDRPELANGWNRAYLYSFRIKPPYRG
                     LGLGTRMVKTLEAYLVARHYSRLTLNVARDNLEAIRLYHRLNFQIVGEEPGIWSYIDH
                     LGKWHTVEEPSWRMEKHLL"
     gene            complement(4621..>5122)
                     /locus_tag="PLW25_03775"
     CDS             complement(4621..>5122)
                     /locus_tag="PLW25_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53641.1"
                     /translation="YAQFSDWNLPDLIGNTTTRHALIYLTWLASLDGNTGLEVANRLR
                     LPRQLQDTLAHIISARQQLDQLAAGLPSQLTLELEKIPLYGVFALYIVCEHSSLKDSI
                     NLYATKWRFIFPGVDGNTLLARGIPPGPKYTSILTAIRDAWLDGRVTNEKEEALLLER
                     LLKEIQ"
     assembly_gap    5123..5222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5223..7421)
                     /locus_tag="PLW25_03780"
     CDS             complement(<5223..7421)
                     /locus_tag="PLW25_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOS53642.1"
                     /translation="MNVVLTHEQADFDALAALLCAHLLNEHAIPVLPNRLNRNVRHFL
                     NLYGDKFPFVEARDLGADSIQQVTLVDTQSLVTLKGINAKTAVNIVDHHDPRTDLPAQ
                     WHTRFEKVGATTTMLVDLLREHARPISIIEATLLLLGIYEDTGSLTYASTTVRDVNAA
                     AYLMEKGASLKIAAEFLNPALSEGQKKLADRLLENAQTINIHGKTIILSCANAVDFNE
                     EIASIAHKLRDLLDPDALFLFVRTVEGIRLVARSTSDQVDVARIAQHFSGGGHERAAA
                     ALIQAAEDAEEVALDVFCHEVIEELERSIEPPLTLNKIMSKKPHLISPSTSLNEAHLL
                     MQRYGYEGYPVVDNGKVVGLLTRRAVDRALSHKLNLNAGSLMEAGEHFLKNTDTIDKL
                     QQLMAATGWGQVPVVDAENGKIIGIVTRTDLLKTLPGHNGIIPGQRNHASMLEKALPA
                     SRLALLKLVAGASEKGQHAIYIVGGFVRDLLLERPSIDFDIVVEGDALQIAQALSQQY
                     GGRVIPHKRFSTAKWFIKPIRANLLKRMGIENGRATDLPEALDFISARTEFYDHPTAL
                     PTVERSSIKLDLHRRDFTINTLALRLDGRHYGELYDYWGGLQDLEKKQVRVLHSLSFV
                     DDPTRLLRAVRFEQRFHFQIEKRTLQLMTEALELLHQLSGERIHHELDLIFNDHDPVS
                     IMRRLEELGLLRHIHPGLRWQGQNDAFFAQAINYAQFSDWNLPDLIGNTTT"
     gene            7483..8259
                     /gene="moeB"
                     /locus_tag="PLW25_03785"
     CDS             7483..8259
                     /gene="moeB"
                     /locus_tag="PLW25_03785"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HOS53643.1"
                     /translation="MELQLCSMELSDLERQRYKKHLQLESIGVEGQLKLKHGRVLVIG
                     AGGLGSPVLIYLAAAGVGNLSILDDDLVTLSNLPRQVIHITNKIGTPKVNSAWERLEQ
                     MNPEISFRPIQERLQPANAESIISGHQVVVDCTDNLDARLLINETCVKASIPFVYGAV
                     FEYEGQVSVFDAKRGPCLRCMLPEQLSAGTIPNPENHGLLSTVPGVIGLLQAAEVIKL
                     LTGIGDTLVGVLALYDALAVNLTKIRIAKRENCPVCGTAA"
     gene            8289..9167
                     /locus_tag="PLW25_03790"
     CDS             8289..9167
                     /locus_tag="PLW25_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOS53644.1"
                     /translation="MTEKKVLLAVLAHPDDETFGTGGSLAHYARAGVEVHLVCATRGE
                     VGEVDPLLMGEFKSIGELRENELLCAAGYLGLAGVHFLNYRDSGMAGSPENHHPQSLV
                     AADQQKVIGEIVHYIRLLKPQVVITFDPIGGYMHPDHIAIHQAADKAFYAAADPALFP
                     SNLPAYQPQKLYYHTFPRGFLRTTVWLMKIAGKDPSRFGKNKDIDLLKISQARFPIHA
                     VVDYRDVIDQKHQAVACHASQGGMRITGGVLDKLRKVFGVSDQYMRAYPPPTRRRERD
                     LFDGVILTDAASENRL"
     gene            complement(9463..10512)
                     /locus_tag="PLW25_03795"
     CDS             complement(9463..10512)
                     /locus_tag="PLW25_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53645.1"
                     /translation="MASTQLTPAEQLNRDIASTRSEITRLVDEVKLTSVRNTYSTLDS
                     AIANLPLRVQKVRARKYAFNKILETQAEEFQKQWALKRSHVLNQITTESNALQSQLRV
                     LEGRAAGIANSAAVKMLQSDITNFKARVSASKRAVTDLFDKLNNEVNKVQSQMSQIEK
                     TLDLAESACFGFLPTESVVRAVKALWTRDEQEDKTDPKGILFLTDQRLLFEQREEIAT
                     KKVLFVTTERKKVQELLFEVPVFSITSVQATKQGMLKNEDWLTLEFESGAFARGAKIH
                     LDGQNCDEWQKLITQVKSRELDSDRAIEIDSQAVEKAKAAPVICPFCGGSITKPVLRG
                     MDSIKCDFCGNVIKL"
     gene            complement(10620..11876)
                     /locus_tag="PLW25_03800"
     CDS             complement(10620..11876)
                     /locus_tag="PLW25_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS53646.1"
                     /translation="MQNDKQEKISSMHILLIHQAFAALDEPGGTRHYEMAKFFAEKGH
                     RVTIITSPVSYLTGKASQARQRWVTRENPEPGIEILRAYTYPALHRSFFHRLISFVSF
                     MFSSFCIGLGVKNVDVVWGTSPPIFQGWTAWLLARLKGRKFLFEVRDLWPAFAVAMKV
                     IRNPLIIRLSNWLERFLYHHADTLVVNSPGFIPHITSRGGSNPVLIPNGVDPAMFDPE
                     DNGSEFRQQLKLEHAFIVLYSGAHGLSNDLGTVLKVAQLTAQDSRIVYVLVGDGKEKA
                     ALQQLAHDLGLQNVIFVPSVAKTEMKKVLAAADACLAILKPIELYKTTYPNKVFDYLA
                     AGRPVLCAIDGVIREVIEEAHAGIFVPPGDPAALADAVIRLSSAPDKGKKMGLNGRAY
                     VVEHFNRRDIAARMEKVLFECANNPR"
     gene            complement(11863..>12827)
                     /locus_tag="PLW25_03805"
     CDS             complement(11863..>12827)
                     /locus_tag="PLW25_03805"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HOS53647.1"
                     /translation="GRLPNLGHNDGTLLLPFGCDEFRDYRPTAQAAAIAFLGEPCLPA
                     GLWDELANWLGLTVKPTQPSLDFHSSPAVRRVGKGTGWGTLRGVRFRSRPAHADQLHV
                     DLWWDGHNIARDAGTYSYNLPPPWQNALDTTSVHNTITVNHQDQMQRMSRFLWLEQAQ
                     ARWLEHNQEGYITANHNGYRRFGITHQRTLQSIHGSGFLVTDRLLKKGENVQLVEYCL
                     HWLLPDWNWQLKDQTLVLHGQPFTLRLIINARQMAPIEALEPKDVSLVRAGHTLVGTR
                     ADIRLGWESDTYSEKHPALSFSLVYNSTRSIELITRWEISDAER"
     assembly_gap    12828..12927
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12928..13964)
                     /locus_tag="PLW25_03810"
     CDS             complement(<12928..13964)
                     /locus_tag="PLW25_03810"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOS53648.1"
                     /translation="MNQLRKLFLLIKELGVADLVSYSLYQAQLRSGYTRKLTPLGGLP
                     APEQFLVSAAPEFCWRSCWERLSGKFSARLMIEEARLLKDGYYRPFYATSQILHLEPP
                     SQSSVHWIDVNEERFDDIKMVWEPARFFWSLTLAGVYRGKAQESAAELFWQKFDEFNH
                     NNTVNAGPNWVSAQEVGMRAMMWIIAISAFHSSSFSTSQRLERLTTAIVHHVERILPT
                     LNYARSQHNNHILSESLCLMLAGDFLANLHPRAREWSRQGESYFNHALLRQVEEDGTY
                     AQHSANYHRLMLQLSLLYHARMRQQNRELPPAVMRKLAAASRWMLAQLDPLSGRLPNL
                     GHNDGTLLLPFG"
     gene            complement(13990..16140)
                     /locus_tag="PLW25_03815"
     CDS             complement(13990..16140)
                     /locus_tag="PLW25_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HOS53649.1"
                     /translation="MKQILQYLNNGKTVVEEVPVPAVRRGMVLVQTACSLVSAGTERM
                     VVDFASKNLVGKATSRPDLVKQVVQKAMREGLFPTVEAAFNKLDQPMPLGYSSSGIVV
                     KAGEDALDFKAGDRVACAGGGYAVHAEYALVPVNLAARLPQNVDFESAAFTTLGAIAL
                     HGFHLASPQLGESVCVIGLGLLGLLSAQIARAAGCLVFGIDLSEERVSLAQALGFSAA
                     QRQQCANLAAGVTNGLGFDHVLICADTPSQDPVELAGDIARDRGTVVAVGAVGMQIPR
                     KRYYEKELVFKISRSYGPGRYDPVYEEGGIDYPPGYVRWTEGRNLQAFIDLMHTGKVD
                     VRPLISHRIPIEDAVSAYDLISGKRSEPFMGILLTYPVKEDFSNQQKVLVTPSAANVP
                     AAIKLGVLGAGNYAQAVFLPVIKKTPGAQLVGIATASGMSAQQAARKFGFEFATTSEE
                     EILDNADINTVVLLTRHQHHARQVLSALRHHKAVYCEKPLALNAEELSEIEAELARAD
                     APLLSVGYNRRFAPLTLTLKNFIAQRTEPLYAHYRVNAGFLPLNHWLHDPEQGGGRII
                     GEACHFIDLLTFLIGAVPVSVSAAALADQAKYRQDNVQLTFRYADGSIGTVAYLANGN
                     KNFPKERIEVFSSGKIGVLEDFRSLALVSENERKTSRSVLRQDKGHAQSWAAFLAAVK
                     KGGTPPIPYDELLNTSRASFAALQALRTATEMKV"
     gene            16276..16941
                     /locus_tag="PLW25_03820"
     CDS             16276..16941
                     /locus_tag="PLW25_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53650.1"
                     /translation="MLKKILWILMLLIMTACTILFKDGVNIVLGELTPTMVATSTPVD
                     IGVKITPTETVEPTMEPTATEEPVNLPSPTIVTTAEPEPTPTPEATATPFVAVYEVQK
                     GSPAYLDNFAHLSDGCAWQGIAGQVFNSKKEPVTNLVVKVSGEWNGKKIASVGVTGMV
                     AGLPYGPGSYEIVLGDKAVNSNATLYVQLFDEGQKPLTEPIAVSTQADCQKNLVILNF
                     VKK"
     gene            17014..17829
                     /locus_tag="PLW25_03825"
     CDS             17014..17829
                     /locus_tag="PLW25_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53651.1"
                     /translation="MAEQNQETSKDLLLSDVFIRAARYWWLVTILMIAGGVAGLLIAL
                     LQKPLYESTSQVTTVIDFAYANRLKDYEEDFLLSAIGDIITSDEVIQATATAAVTLGL
                     FTNPDEVTRGMTASRQGYRWQLSTRFNDAHASQKVNQLWLDSSMRALENLRQQSIKAL
                     AQINTEAQVQACFQQSVVLEPVSSYCTVDEIRALLTQPESSNNATSSLSLLNRLLISR
                     ISFQATREAELPDQPVHIGLNTATLAGAAIGLLIAIILFVLGYPRVLRTGCQK"
     gene            17826..19274
                     /locus_tag="PLW25_03830"
     CDS             17826..19274
                     /locus_tag="PLW25_03830"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOS53652.1"
                     /translation="MSSRFWLKSNWYCWALLVITLPITTFPVVMKLTGSASVAPAALL
                     PLIPLVLFFLPAYFWQKKALPVQAKTVLLFFLFALFTIALAFLRPLPLYKAQSPLAAG
                     MKGVVTLLLGFLFYLVAVSVPSSPEKIDKTLRLVNWSGLVVVLWSILQIVLEPLFPAS
                     KVALNQIQSFISPTRLLDDRMQGFASEPSWLAHMLNLVFLAYWLAASYNRTSVHLFRI
                     WRFSFENLLALLGIVALAGTLSRGGILAFLLVLAVIFVLLNIRMVKSLIRKWGQPKNG
                     FRIAGITLGLVLVYLSLIAAALWGWSRLDARMEKVFLLSTKGENPLIEYADNLRFGER
                     VIYWQTGWNIFNDHPLIGVGVGFSGFYFPEYLPDAGWGLSETRKLMFRSEGLLNIKNL
                     WSRLLAETGIIGFALFLTFLVLFAFTSLEMVVKGDGRRKTLGFMGFFMLAALVIEEFS
                     VDSFALPYLWFSMGLVTAGWRWYTFKNGGEHG"
     gene            19267..>20224
                     /locus_tag="PLW25_03835"
     CDS             19267..>20224
                     /locus_tag="PLW25_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53653.1"
                     /translation="MDKSEKLVLLVFLVILVSACCLLVACGGMALLLSSVSGFLSPTA
                     DTVTFADGTPTPGSPSSVIQPSPMATKLSSEGFERIYHTLSTLENTFIPSADLIYLAE
                     RFEGKKGIPRQLTTPPVAYEVGDRLDFYKINTDTNVSSVTTAVLRYASDTLYFWVEEG
                     VKIDQKELQKTLDVFESKIYPTNQEFFGTEWIPGVDNDPHLYILYAGDLGYSLAGYNA
                     SSDGVLPLAYKNSNAHEMFYINSDVQTLSDPYTLSVMAHEFQHLIHGYHDPNEELWIN
                     EGFSELATLLNGYSAGGFDYLFSYDTDVQLNDWSTESGKNDVH"
BASE COUNT         4704 a         5025 c         5446 g         4849 t
ORIGIN      
        1 agacaatcac cggaacggtt atacacggta tactgaactc agtaagccag ccgctcaatc
       61 tattggggta ggtagtacaa tcattacagg aactcttttt atgctgaaaa cgtcttttca
      121 taaatggcgt ttactcctgg tttctctgct ggctttggca aacagctctc cgatgtttta
      181 ttcgttatgg agttatagcg gaggagagat tcaccgatta acgggaactg agacgaaact
      241 taaagttaca agcggtggga tgcaggcaac cagcacgaac agcggagagt tgtgtgtgct
      301 ttttcaagag gatcatgtga aggttgacgg ctgtgataag gatagcccca tcaccccctg
      361 gcaaagcccc ccggaatggg aggtgagaga ggtgatcgtt tctgacctca attgggataa
      421 ccaggaggag atcgcaatgc tggtgtggcg tcctttcaag ccctggccag ttgaccgctt
      481 tatgccaagc gcgggacgca ttaacacctt tcatgataag aacgatatga gctgtcacct
      541 gattctcatt gggctgcgaa acgggaatat taaagaattg tgggctgggt cagcgatggc
      601 tgacccgcta agggaaattc acgcagccga cctggatgga gatggacggc aggaactcgt
      661 tgctcttgaa catgcctata acggaaaccc tcgtgcatct tctatcacgg tgtgggcatg
      721 gcatgggttt ggtttttctt tgcaagccag gcagaatgga aggttttcat ctctgcttgt
      781 actaccagcc gaacaaggga aagtgatatt gactcaatag gaccctcaag gaggaaaaaa
      841 tgaaccgctt gtttaaagta ctctctgtgt tgctcatcgt cgcatttcta ctcagtgcct
      901 gccagctgcc attcaacctg gtggaaagga ttaaaaatgc attgattgag aaagggcaat
      961 cgggcgaggt taccgaaccc gccgcccagg gcactgaaac gacgaccacc cagccgccgg
     1021 tggtataccc agcagaagga atggctcccg tgttcgcagc ctatcaacag cgaccgtata
     1081 ccatgcctga ggtttaccag gggggttaca cccttccttt aaaagccagc gagttggtca
     1141 accttgactt gtttgatctg actgactctc aggttgaggc gctgctcaac aacggttttg
     1201 tcgttcagcc gccgaacaat gaccctagcc gcatgtacaa cgagttctat caggcatatg
     1261 agtccatccg ttatgaggaa actccggttt ttgtcaccac ggatgctatc tttcacgtat
     1321 atcatcttat ctttgataag atgctgcgcg atttggaacg cacttttttc attgacacct
     1381 tgaaagaact gacaaccgcc atggtcactg aatctgtggc tcaatacgaa aaactgcaag
     1441 gtagtcctct ggaagaagcc gcgattcgta atgtggcttt cttcacggtt cccgcgatga
     1501 ttcttgacct gccggtgagc gtaccagctg aggctaaaaa attggccgaa gaggagctcg
     1561 cgttgatcaa tgaaggtggt ggcatttcag cttcaccgat ttggaagaca gagggacagg
     1621 cgactgacaa tgtcctttta gaggattact ctcagtacat cccgcgtggg cattacacgc
     1681 gcgacgaggc gctgaaaacc tactttaaag cgatgatgtg gtttgggcgt atgacctttc
     1741 gcctgatgga cccgattgaa acgcaacgcg cactgctggt ggtgcaggcc atgcgtaacg
     1801 cgtctacaac ttcaggcaaa ccagcgcttg agctctggca aaacatttac gaccccactg
     1861 tttttatcgt gggaaaagcg gatgacctat caatctacga gtatggaaag ctatccgatg
     1921 aggtctttgg tgttcaccca gagctgacct cttttgcaga ccccttactg gcagagaagt
     1981 tcacccaggc agtgaagcag ctacctcctc cgcagattaa ttccatgtgg gtctacatct
     2041 ggcaggaacg cgacgcagtc acccagggat tccgctttat ggggcagcgc tttacgctgg
     2101 accaatatgt gtttggaaaa ttgatctggc gaaaggtggg cacgacggaa aacccgcgcg
     2161 acctgcctaa aagcctggat ttcctcgctg cgcagggttc agaagaagcc tacaatctgc
     2221 ttgccgaaat gggtgaaacg aaatttgaga actacgattc gcagctagcc aaagtgaaag
     2281 aagaggtggc cgggtttggt atcgattcct ggacgcagaa cctttactgg tcatggctgt
     2341 atgcgctgca gccaatcttt gcccccaaag gcgagcaata cccgccattt atgcgcaccc
     2401 aggcctggct gcgcaaggat atgcagaccg cgctcagttc gtggactgaa ttgaagcacg
     2461 ataccatcct ctatgccaaa caggtgatgg ctgagatggg cggaggggca ttggacaagc
     2521 cacccagggg gtacgtggaa cccaacctcg aggcttacgc gcgcttgctg gcgttaaccg
     2581 aaatgacgcg cagcggcctg gaaaaccggg agattttgga tgatgtgacc cgcgggaatc
     2641 tggataacct gatcgaagaa ctggtcttct tgctggacgt ttcacaggcc gagctgaatg
     2701 gggtgaagct gacagaagag caatattggc gcatccaata ttttggcggc tggttagaat
     2761 ccatgaccat cgctgcttca gaccctgctg atgttgcgtt ggggggcggc aacctttcgg
     2821 accagaagtc tgccctggta gcggatgtgg cgaccgggat gggtcgggtg ttagaggaag
     2881 gagtgggata cccaaccaag atttacgtgg tgcaccccga agcaccatac gaaattgctg
     2941 ttggcgcagt ttacacttat tatgagttcg atgtcccgct ggctgaacgc cttacagacg
     3001 aagcctggca gaaaatgctt gagacaggca aggctccagc ggctccggaa tggacaggta
     3061 tgttcatcgt gaaatgatga aattgcgttc gagcgtacaa ggagcgctga tactggcgct
     3121 ccttgtctta acaggatgcc atcgcgctag cgacagttct tcgctgttct tgggcggaga
     3181 tgtgttgctc tatagggcag ggaagcctct ttttccggta gaggatgaag ccatttctcc
     3241 atggggggat ttcataaagg tcaaacagga ggaacatgca gacctgtttg cggtcaacct
     3301 tgaatctcct tttggattga gggtggttga gcatggtttg gaaaatccat cgatgaacct
     3361 ttgcgcggat gatgacgcgc tgcagcattt gcagcaggct ggggttaacc tcatcacaaa
     3421 tcacaacaat catgtcaatg attgcggatt ctccggtcaa cagtatacag atgaattact
     3481 ggaccgggct ggcattcttc accagagaaa tcatagttca gtccagtttg tcccagcggg
     3541 gggcgcggtt atcgccgtgt tgagcataaa tgactacgaa agagattacg aaattgtcac
     3601 ggtggttaac gagttgaaaa aagcccgcgg agcaagcgat ttggtgctcg tttcggtgca
     3661 ctggggacag gagtaccagg caggaccatc gcaacaccag caggaactgg cacagttgct
     3721 ggtagatgcc ggagcggatg tgatttgggg gcaccacccg catgtgctgc agcgtatgga
     3781 atggctgcgt tcttcggcag acgggcacac gggattggtg atgtacagcc ttggcaacgc
     3841 gttaagcgac cagtggatgc tgccagacgc acagcgcacg gctctaattc gattgacatt
     3901 ttctcatcac caggtaaaag acatcctggt tgttccactg aagatggata tgtcctcgcg
     3961 aacacttctg ctggaaaaag acccacaatt attcaactgg tatgttgaac gcctgtcgct
     4021 ggagtctttg gaaaagaaag gaacgaccat ccgattgttt gaattcgcgc aaaccccgta
     4081 gccttcaaag aaggtgtttc tccatgcgcc atgaaggttc ttcaacggtg tgccatttgc
     4141 ccagatggtc gatataagac cagattcccg gctcttctcc cacgatttgg aaattcaggc
     4201 ggtgatacaa gcggatcgcc tccaggttat cacgcgcaac attcaaagta aggcgcgaat
     4261 aatgacgggc aaccaggtaa gcttcaagag tcttaaccat tctggttccc aatcccaacc
     4321 cgcggtaagg cggtttaata cggaacgaat aaagataagc cctgttccag ccgttggcaa
     4381 gctcaggtcg gtcgcaatcc agttgcaaaa aaacctgtcc cacgatgcca tccacaggtg
     4441 attctgccac ccaggctgcc gcggtgccaa gccgggtgcg ctggtaaacc ccgcggtaca
     4501 cattacggaa gtgtgtgtac tcgccatccc attccaacgc gggcagatcc ttctttttca
     4561 gctcacgaac cagaatctca tccagcggcg cccgggaggg gtggtcagtt gagagatccg
     4621 tcattgtatc tctttgagga gcctttccag taataatgct tcttccttct cgttagttac
     4681 ccgcccatcc agccaggcgt cacgaatggc ggtaagaatg ctggtatatt tcggtccagg
     4741 cggaataccg cgcgccagca aggtgttgcc atcaaccccg gggaagatga accgccattt
     4801 cgtggcatac aggttgatgc tatcttttag tgaagaatgc tcacacacga tgtaaagagc
     4861 gaaaactcca taaaggggta ttttttccag ttcaagcgtt agctggctgg gtaaccctgc
     4921 agccaactgg tccagctgct gccgcgcgct gatgatatgc gccagcgtat cctgcaactg
     4981 gcgcggcagg cgcaacctgt ttgcaacttc aagccctgtg tttccgtcga ggcttgccag
     5041 ccaggtgaga taaatgagcg cgtggcgggt ggttgtgttg ccgatgaggt caggtagatt
     5101 ccaatctgaa aattgggcgt agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnggtggttg tgttgccgat gaggtcaggt agattccaat ctgaaaattg ggcgtagttg
     5281 attgcctgag cgaaaaaagc gtcattttgc ccctgccagc gcaagccagg gtggatgtgg
     5341 cgcagcaggc ccagttcttc aagccgacgc ataatggata ccgggtcgtg atcattaaaa
     5401 atcaggtcta attcgtgatg aatacgttcg ccgctcaatt gatgcagcag ttcaagcgct
     5461 tcagtcatca actgtagagt tcgtttttca atttgaaaat ggaaacgctg ctcaaatcgc
     5521 accgcccgca gcaggcgggt tggatcatct acgaacgaga gggaatgcag caccctgacc
     5581 tgtttctttt ccaggtcctg gagacctccc cagtaatcat aaagttcacc gtaatgacga
     5641 ccatccaatc gaagcgccag ggtatttatt gtaaagtctc tgcgatgcaa atccagcttg
     5701 atgctagacc gctcgacggt aggcagggcg gtggggtggt cgtagaactc ggtgcgcgcg
     5761 ctgataaaat ccaaggcttc ggggaggtcg gtggcgcgac cattttctat acccattcgt
     5821 ttgaggaggt ttgctcgaat tggcttaata aaccacttgg cggtactgaa gcgtttatgc
     5881 gggatcacgc gaccgccata ttgttgggaa agcgcctgcg cgatctgcag agcatcgcct
     5941 tccacgacga tgtcaaaatc aatcgaaggg cgttctaaaa gcaggtcgcg gacaaatccg
     6001 ccaacgatat aaatggcgtg ttgccccttt tctgaagcgc cagctacaag tttcagtaaa
     6061 gccaacctgg atgccggcag ggctttttcc agcatggacg cgtgatttct ctgcccgggg
     6121 atgataccgt tatgcccggg gagggttttg agcaggtcgg tgcgggtaac aatgccgatg
     6181 attttgccgt tttcggcatc taccactggc acctgccccc aacctgtagc cgccattaat
     6241 tgctgtagtt tatcgatcgt atcggtgttt tttagaaaat gctccccggc ttccatcagg
     6301 cttccggcgt taagattcaa tttatggctc aaagcgcggt caaccgctct tctggtgagc
     6361 aaccccacca ctttgccatt atcaacgaca ggataacctt cgtatccata acgctgcatt
     6421 aataaatggg cttcattcaa tgaagttgaa ggactgatga ggtgcggctt tttggacatg
     6481 attttattga gagtcagggg tggttcaatg gagcgttcaa gttcctcgat aacctcgtgg
     6541 cagaaaacat ccaaggcgac ctcttcggca tcctctgccg cctggatgag cgccgctgcg
     6601 gctctttcat gccccccacc gctaaagtgc tgggcgatac gcgctacatc tacctggtca
     6661 gaggtggagc gcgccaccag cctgatccct tcaacagtcc gcacgaaaag gaagagcgca
     6721 tcagggtcta acaggtcgcg caatttgtgg gcaatactgg caatttcctc gttaaaatca
     6781 accgcgttag cgcacgagag gatgatggtt ttgccgtgga tattgatcgt ctgggcgttt
     6841 tccaataacc ggtcagccag cttcttctga ccctcagata gcgccgggtt cagaaattca
     6901 gcagcgatct tcaggctggc gcctttttcc atcaggtagg cagccgcgtt gacatcacgc
     6961 acagtggtgg aggcgtaggt taacgacccg gtatcttcat aaattcccag cagcagcagc
     7021 gtggcttcga tgatggagat gggccgggcg tgttcgcgca acaggtcgac gagcatcgtg
     7081 gttgtggcgc cgaccttttc aaaccgggta tgccactgcg cgggcaggtc agtcctgggg
     7141 tcgtgatggt ccacaatatt gaccgcggtt ttggcgttga tgcctttcag ggtgaccagg
     7201 gattgcgtat caaccagggt cacctgctgg atgctatctg cgcctaaatc ccgcgcttca
     7261 acaaagggaa atttgtcacc atacaggttg aggaaatggc gcacgttacg gttcaggcgg
     7321 ttggggagaa caggaatggc atgttcattt aagaggtgcg cgcacagcaa agctgccagc
     7381 gcgtcaaaat ccgcctgttc atgcgtgaga accacattca taatttgatt gtaacatcaa
     7441 ggcgcatatc tgttacgatg agcagtttca gaaaattata gaatggaatt acaattgtgc
     7501 agtatggaac tgagtgacct ggaaagacag cgttataaaa aacacctcca gctggagtcg
     7561 attggggttg aaggtcagtt aaagcttaaa cacggacggg tgctggtgat cggcgcgggc
     7621 ggattgggct cgccggtatt aatttatctg gcagccgcgg gggtaggcaa cctatctatt
     7681 ctggatgatg acctggtcac gctctcgaac ctgccgcgcc aagtcattca tatcactaac
     7741 aagattggca caccgaaggt aaattctgcg tgggagcgcc tggagcagat gaacccagag
     7801 atcagctttc gaccaatcca agagaggctg caacccgcaa acgccgaatc aatcatcagc
     7861 ggtcatcaag tggttgtgga ttgcacagac aacctggatg cgcgattgct gatcaatgaa
     7921 acctgcgtca aggcctccat tccctttgtt tatggcgcgg tttttgaata tgaaggacag
     7981 gtttccgtct ttgatgcaaa acgaggacca tgcctgcgct gtatgttacc agagcagctt
     8041 tctgcgggca ccataccaaa cccggaaaat catggattgc tctctacggt ccccggagtg
     8101 attggactgt tacaggcggc ggaagtgatt aaattgctga caggaattgg cgatacgctg
     8161 gttggggtat tggcgcttta tgatgccctg gcggtaaacc tcacaaaaat caggatagcg
     8221 aaacgcgaga attgccccgt ttgcggcacg gcggcgtaat caaaccaatg gaacaggata
     8281 gagcaagaat gacagaaaag aaagtgctac tggctgtact ggctcaccca gatgatgaga
     8341 cgtttggcac cgggggatca ctggcgcatt acgccagggc aggggtggag gttcacctgg
     8401 tttgcgccac acgcggagaa gtgggagaag tggacccgct gctgatgggt gaatttaaaa
     8461 gtattggaga actgcgcgag aatgaactgc tctgcgctgc gggctattta gggctggctg
     8521 gggttcactt tttaaattat cgtgattccg ggatggctgg atcgcctgag aatcatcatc
     8581 cacaatcgtt ggtcgcggcg gaccagcaaa aagtgatcgg tgagattgtg cattacatcc
     8641 gactactgaa accccaggtg gtcatcactt ttgaccctat tggtgggtac atgcaccctg
     8701 accatatcgc catccaccag gctgcagaca aagcctttta cgcggctgca gacccggcgt
     8761 tatttccatc taacctgccg gcttaccagc cacaaaaatt gtattaccat acttttccac
     8821 gcggtttttt gcgcaccacg gtctggctga tgaaaattgc cggaaaggac cccagccggt
     8881 ttggcaagaa caaagatatt gacctgttga aaatttcaca agcaaggttt cccatccacg
     8941 cggtggtgga ttaccgggat gtgattgacc aaaaacacca ggcagttgcc tgccacgcca
     9001 gccagggggg catgcgcatc accggcggcg ttttggataa gttgcgcaag gtttttggcg
     9061 tgagcgacca gtatatgcgg gcttatccgc cgccaacccg ccggcgtgag cgggacctgt
     9121 tcgatggcgt aatattgacc gacgccgcat cagaaaacag gctgtaaaca acgagctgaa
     9181 gacaaggtgc ggtgcggatg tgctcctttc gacgagccgg ttgctcacta ttttgcttca
     9241 aacacaatgg cagacggtga gatctctcct ggcggttacg cggcggtctc accgcggtta
     9301 cccgaacaat gggacgttct ctaacccagc gcattccact ccgtttggtt gttcaaaccg
     9361 cccaaacggt ggcgggtgat ttcttactcc gttacctcta aatgatagca aacatacctc
     9421 cgcagctacg gcgggcttca gcggaatgcg aaagaaagga ggttagagtt tgattacatt
     9481 gccgcagaaa tcacacttga tgctatccat gccgcgcaga acaggtttgg taatcgagcc
     9541 gccgcaaaac gggcagataa ccggcgcggc tttggctttc tctaccgcct ggctgtctat
     9601 ttcaatggcg cggtcactat ccagctcgcg tgatttgacc tgcgtgatca gtttttgcca
     9661 ttcatcgcag ttttgaccgt caaggtgaat tttcgcgccg cgcgcaaacg cgcctgattc
     9721 gaattcgagg gtgagccaat cctcattctt caacataccc tgtttggtcg cctgcacgga
     9781 agtgatggaa aacacgggaa cttcgaacaa cagctcctga accttcttgc gctcggtggt
     9841 cacgaaaagg actttcttgg tggcgatttc ttcgcgttgt tcaaagagca agcgttggtc
     9901 agttaaaaac aggattcctt ttgggtcggt cttgtcttct tgctcatcac gagtccaaag
     9961 ggctttcacg gctcgaacca cgctttctgt gggtaaaaag ccaaaacagg ctgattccgc
    10021 caaatcgagt gtcttttcaa tctggctcat ctgagactga actttattca cttcgttatt
    10081 caacttgtca aataagtcag tgacggcgcg ttttgaagcg ctaacacggg ctttaaaatt
    10141 tgttatatct gattggagca tcttcactgc cgcgctattg gcaatacctg cagcgcgacc
    10201 ttctaaaacg cgcaactggc tttgcagcgc gttagattct gtcgtgatct ggttaagtac
    10261 atggctgcgc ttaagcgccc attgtttctg gaactcttct gcctgggttt caagtatctt
    10321 attgaaggcg tatttacgag cgcgtacttt ctgcacgcgc agcgggagat tggcgatggc
    10381 gctgtcgagg gttgaatagg tattccggac tgaggtgagt tttacctcgt caacaagcct
    10441 ggtgatttcg ctgcgtgtgg aggcgatatc tctatttaat tgttcggcag gtgtgagctg
    10501 tgttgaagcc atgggattcc tcctgtggga tgagatgcaa atgattaccc actattgtac
    10561 cttattcgat taccaatctt tttggaccgt cgtgacagag gtgtgaaaac aagccggggt
    10621 tatcttggat tgttggcgca ttcgaaaaga actttttcca tacgcgcggc gatatcgcga
    10681 cggttgaaat gctccaccac gtaagcgcgg ccatttaacc ccattttctt tcccttgtct
    10741 ggtgcggatg agagccttat gactgcgtcc gcgagcgcgg cagggtctcc cggagggaca
    10801 aaaatacctg cgtgggcttc ttcgatcacc tcgcgaatca cgccatcaat ggcgcataaa
    10861 accgggcgac cagcagcgag gtaatcaaaa accttgttgg gataggtggt tttatacaat
    10921 tcgattggtt taaggatcgc caggcaggca tcggcagcgg caagaacctt tttcatttcc
    10981 gttttcgcca ctgatgggac gaagatgaca ttctgtaaac caaggtcatg ggcgagctgt
    11041 tgtaaggcgg ctttttcttt tccgtcaccc accagcacgt aaacaattcg gctgtcttgc
    11101 gcagtaagct gcgctacctt cagcactgtg cccaggtcgt tagataaccc atgcgcgccg
    11161 gaataaagga caatgaaagc gtgctccagc ttcagctgct ggcgaaactc actgccgttg
    11221 tcttcagggt cgaacatggc tgggtcaacg ccgttgggta tcagaacagg gttgctacct
    11281 cctcggcttg tgatatgagg gataaaccct ggtgaattga ccaccagtgt gtccgcgtga
    11341 tggtaaagga agcgctcaag ccagttagac aggcgaatga tcagcgggtt gcgaatgacc
    11401 ttcatcgcca ccgcgaacgc tggccagagg tcacgcactt cgaaaaggaa ttttctgcct
    11461 ttcagccggg ccaacagcca ggcggtccat ccctgaaaaa tgggagggga ggttccccaa
    11521 acgacatcca cattttttac gccaagacca atacaaaagg atgaaaacat gaaactgacg
    11581 aagctgatca ggcggtggaa gaaactgcga tgcagcgctg gataggtgta agcgcgcaaa
    11641 atttcgatgc ctggttccgg gttttcccgt gtaacccagc gctgcctggc ctgactggct
    11701 ttgccggtga ggtagctcac cggactggtg atgatcgtga cgcgatgacc tttttcggcg
    11761 aaaaatttgg ccatctcgta gtgccgggtt ccacctggct catccaaagc ggcaaaagcc
    11821 tggtggatca ggaggatgtg catcgagctt atcttctcct gcttatcgtt ctgcatctga
    11881 gatttcccat cttgtgatca gttcaatcga cctggttgaa ttgtagacca gcgagaaaga
    11941 aagcgccggg tgtttttcag aataagtgtc cgattcccat cccaaacgga tgtccgcacg
    12001 cgtgccaacg agcgtatgac ccgcgcggac aagcgagaca tcctttggct cgagcgcttc
    12061 aatgggcgcc atctgtcgcg cgttgatgat caacctgagc gtgaacggct gcccatgtaa
    12121 gacaagcgtc tggtctttta attgccagtt ccaatccggc aacagccagt gcaggcagta
    12181 ctccacgagt tggacatttt cgcccttttt tagcagacga tcggtcacga gaaagcctga
    12241 cccgtgaatg gattgcaggg ttctctgatg ggtgatgcca aaccggcggt agccattatg
    12301 gtttgcagtg atgtagccct cctggttgtg ttccaaccat ctggcttgcg cctgttccaa
    12361 ccataaaaaa cggctcattc tttgcatctg gtcttggtgg ttgaccgtta tcgtgttgtg
    12421 cacgctggtg gtatccagcg cattttgcca cggcggcggc aggttatagg aataggtgcc
    12481 ggcatcgcgc gcgatgttgt gcccatccca ccagaggtcg acgtgcaact ggtcggcgtg
    12541 ggcgggacgg ctgcggaaac gcacgccgcg caaagtcccc cagcctgtgc cttttccgac
    12601 ccggcgcact gccggcgaag agtgaaaatc cagggaaggt tgggtgggtt ttactgttag
    12661 acccagccag ttggccagct catcccagag acctgctgga aggcaaggct caccgagaaa
    12721 tgcaatggca gcggcctggg cagtagggcg gtaatcccgg aattcatcgc agccgaaagg
    12781 caacagcaac gtaccgtcat tatgccccag gttgggcagc cggccgcnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnccg aaaggcaaca gcaacgtacc gtcattatgc
    12961 cccaggttgg gcagccggcc gctgagcggg tcaagctggg cgagcatcca acggctggcg
    13021 gcagccagtt tacgcattac agccggcggc agctctctgt tttgctgacg catccgcgcg
    13081 tggtagagca acgacagctg cagcattaaa cggtgataat tggcgctgtg ttgagcatag
    13141 gtgccatcct cttccacctg ccgcagcaag gcgtgattaa aataactttc gccctggcgg
    13201 ctccattccc gcgccctggg atgcagattg gcgaggaaat cgcccgccag catcaagcaa
    13261 agggactcgc tcaagatatg attgttgtgc tgagatctgg cgtagttcag ggtgggcaga
    13321 atacgctcca catgatgaac tatcgctgtg gtgagtcttt ccaaccgttg cgaggttgaa
    13381 aaagacgaag agtggaaggc ggagattgcg atgatccaca tcatcgcccg catgccaacc
    13441 tcttgggcgg atacccagtt aggcccggcg ttcactgtgt tgttgtggtt gaactcgtcg
    13501 aacttttgcc aaaagagctc agccgcagat tcctgcgctt taccgcggta gacgcccgcc
    13561 agggtcaggc tccaaaaaaa acgcgccggt tcccacacca tcttgatatc gtcaaagcgc
    13621 tcctcgttca cgtcgatcca gtggacactg gattgcgaag gaggttcaag gtggagaatt
    13681 tgcgaggtcg cgtagaacgg gcggtaatat ccgtctttga gaaggcgcgc ttcttcaatc
    13741 atcaaccttg cggaaaactt accggagaga cgctcccaac aggagcgcca gcaaaattca
    13801 ggcgctgcag atactaaaaa ttgttcaggc gcgggtaaac cgccgagagg agtgagtttg
    13861 cgcgtgtacc cactgcgcaa ctgcgcctgg tagagcgaat aactgaccag gtcagccacc
    13921 ccgagttctt ttatcagcag gaaaagttta cgcagttgat tcaactcgat tcgtccgctt
    13981 taaggcggct caaaccttca tttcagtggc agtgcgcagc gcttgaagcg ctgcgaagct
    14041 tgcccgcgag gtgttgagta actcatcata aggaatggga ggagtaccac cctttttaac
    14101 cgctgccagg aaagcagccc aggattgcgc gtgacctttg tcctggcgca gaacggagcg
    14161 tgatgtcttt cgttcatttt ctgaaaccag cgcaagcgaa cggaaatctt ccaatacgcc
    14221 aatcttgccg ctggaaaaca cctcgatcct ctccttggga aagttcttgt tgccgttcgc
    14281 caggtaagcc actgtgccga ttgagccatc cgcgtaacga aaggtcagct gcacgttgtc
    14341 ttgccggtac ttcgcttgat ctgccaatgc cgccgcgctc accgatacag gtacagcgcc
    14401 aattagaaag gtgagcagat cgatgaaatg gcaggcttcg ccgatgatac gcccgccgcc
    14461 ctgctctggg tcatgcaacc aatgattcaa tggcagaaag cccgcgttta cccggtaatg
    14521 ggcatataaa ggctccgtcc gctgagcgat gaagttttta agagtgaggg tcaacggggc
    14581 aaaacggcgg ttataaccca cgctgaggag tggagcgtct gccctggcga gttctgcttc
    14641 gatctctgaa agttcttcag cgttcaatgc cagcggtttc tcgcaataca cagctttgtg
    14701 atggcgtaac gcgctcaaca cctggcgggc gtgatgttga tgccgtgtga gcaacactac
    14761 cgtgttgata tccgcgttgt ccagaatctc ctcttctgag gttgtggcaa attcgaaacc
    14821 gaactttctg gcagcctgtt gggcgctcat acccgaagca gtggcaatgc ccaccagttg
    14881 cgctccgggg gtttttttga tcaccggcag aaaaaccgcc tgggcgtaat ttcccgcgcc
    14941 caacacgccg agcttgatgg cggcaggcac gttggcggct gacggcgtaa ccagcacttt
    15001 ctgctggttg ctgaagtctt cttttaccgg gtatgtgagg agaatgccca tgaaaggctc
    15061 tgatcgtttg ccagagatga ggtcataagc gctgacagcg tcttcgatag gtatgcggtg
    15121 agaaataaga gggcgcacgt ccaccttgcc agtgtgcatc aggtcaatga acgcctgtaa
    15181 atttcgtcct tctgtccagc gcacatagcc tggcggataa tcgattcctc cttcttcgta
    15241 gacagggtca tacctgccag gtccgtaaga acgggaaatc ttaaaaacca gttctttttc
    15301 atagtagcgt tttcgcggga tttgcatccc cacggcgcca acagccacca ctgtaccgcg
    15361 gtcacgagcg atatcccctg ccagttcaac tgggtcctgc gaaggcgtat ccgcgcaaat
    15421 caacacgtgg tcaaaaccga gcccgttggt cacgccggcg gccaggttcg cgcattgttg
    15481 tcgttgggcg gcggagaaac ccagcgcttg cgccaggctg acccgctcct cactgagatc
    15541 gataccaaat acgaggcagc ctgcggctcg cgcaatttgg gcggagagca aacctaacag
    15601 acctaaaccg atgacacaaa cactttctcc caattgggga gaagccaggt ggaagccatg
    15661 cagcgcgatc gcgccaagag tggtgaaggc ggcagattcg aaatctacat tttgcggcag
    15721 gcgcgctgcc aggttcaccg ggacgagcgc atactccgcg tgcacggcat aaccaccacc
    15781 tgcgcaggcc acccgatcgc cggctttaaa atccagcgca tcttcacccg ccttgacaac
    15841 tatccctgac gaggagtatc ccaacggcat tggctgatca agtttattga aagccgcttc
    15901 tactgtggga aagagtcctt cgcgcatggc tttttgaacc acctgcttaa ccaggtctgg
    15961 tcgagaggta gctttaccaa ccaggttttt tgaggcaaaa tcgacgacca tacgctctgt
    16021 tcccgcagaa acgagagaac aggcggtttg caccagcacc atgccgcgcc tgacggcggg
    16081 aacaggcact tcttcgacaa ccgtttttcc attgtttaag tactgaagta tctgtttcat
    16141 ggtttctcct atatcagatt atattcgatt atctgcagcg ttgaccttct ccctcattcg
    16201 cctttttgaa acatgcagaa tggcatggaa agtgcattaa aaacccttac agctcgtgca
    16261 aggtcggagg aaggtatgtt gaaaaagata ctgtggatat taatgctgct tatcatgacc
    16321 gcctgcacca tcctgtttaa agatggtgtg aatattgtat tgggtgagct cacacctacc
    16381 atggtcgcta catcaacccc ggtggatatt ggcgtgaaaa ttacacccac cgagacggtt
    16441 gaaccaacaa tggaaccaac ggctactgaa gaaccagtta atctgccttc accgacaata
    16501 gtaacaacgg ctgaaccaga acccaccccc acgcctgaag caacggctac cccttttgtc
    16561 gcggtttacg aagtgcaaaa gggctctcca gcctacctgg ataatttcgc tcatctctcc
    16621 gatgggtgcg cttggcaggg gatcgcgggt caggtgttca acagtaaaaa ggagccggtt
    16681 acaaacctgg ttgtgaaggt cagcggtgaa tggaatggaa agaagattgc cagtgttggt
    16741 gttacaggaa tggtcgccgg cctgccttac ggtccgggca gttatgagat tgtgctggga
    16801 gacaaggcgg tcaattcaaa cgccaccctg tacgtgcagc tttttgatga agggcaaaaa
    16861 ccattaaccg aaccgattgc tgtttcaact caggcggatt gccaaaaaaa cctggtgatc
    16921 ttaaattttg tgaagaaata atacgaaagc gcgcattgag cgcaggcccc aggtgatgcc
    16981 acgcgtgttc tgtggcagtg tataattcag ccaatggcag agcaaaatca agagaccagc
    17041 aaagacctgc tgctgagtga tgttttcata agggcagcgc gttattggtg gcttgtgacc
    17101 atcctgatga tagccggggg ggtagcaggt ctgctgattg cgcttttaca gaaaccgctc
    17161 tatgaatcca cttcccaggt caccacggtg atcgatttcg catatgccaa ccgcctgaaa
    17221 gattacgaag aggattttct tctctctgcc attggagata ttatcacctc agacgaggtg
    17281 atccaagcaa ctgccaccgc tgctgttaca cttggcctgt tcacgaaccc ggacgaagta
    17341 accaggggaa tgacggcttc aaggcagggt taccgttggc aattaagcac tcgcttcaac
    17401 gacgcgcatg cctcccaaaa agtgaaccag ctatggctgg attcctcgat gcgggcgctt
    17461 gaaaatctcc gccagcagtc catcaaagcg ctggcgcaga taaacacaga agcgcaggtg
    17521 caagcctgtt ttcaacagtc tgtagtcctc gagcccgttt cttcctattg cactgttgat
    17581 gaaatacgcg cgctgctgac ccagccagaa agttcaaaca atgccacctc gtcgctttca
    17641 ttactgaaca ggctgttaat ctctcgtatc agctttcagg caacgcgcga ggctgagctg
    17701 cctgaccaac ccgttcacat tgggttgaac accgccacac tcgctggcgc ggctattggg
    17761 ctgcttatcg ctatcatcct gttcgttttg ggttacccgc gggttcttcg cacaggctgc
    17821 cagaaatgag ttcccggttc tggttaaaaa gcaactggta ttgttgggcg ctgctggtga
    17881 tcaccctgcc catcacaact tttccagtgg tgatgaaact gactggctcc gcttcagtcg
    17941 caccggcggc gttactgcca ttaattcccc tggtgctgtt ctttttgcct gcttattttt
    18001 ggcagaaaaa ggctttgcct gtccaggcaa aaacggtgct gttgttcttc ctgttcgcgc
    18061 tgttcactat cgcgctggct tttctacgac cattacctct ctataaggcg caatcgccgc
    18121 ttgcagcggg gatgaagggc gtagtgacct tgttgttggg atttcttttt tacctggttg
    18181 cagtcagcgt tcccagttcg cctgaaaaaa ttgacaagac cctgcgcctg gtcaattgga
    18241 gcgggctggt ggtggtgttg tggtccattc ttcagattgt tctggaaccc ttgttccccg
    18301 cttccaaagt tgcccttaat cagattcagt ctttcatttc acctaccagg ctgctggatg
    18361 accgcatgca gggttttgct tctgagcctt cttggctggc ccatatgctc aacctggttt
    18421 ttctggccta ctggcttgct gccagttaca atcgcaccag cgttcacctg ttcaggatct
    18481 ggaggttttc ttttgaaaac ctgctggcgc tgctgggcat tgtggcgctc gcggggacgc
    18541 tctcgcgtgg cggaatttta gcttttctcc tggtgctggc agtgatcttt gtattgttga
    18601 atatcaggat ggtcaagtcg ctgatacgaa agtggggtca acccaaaaac ggtttcagaa
    18661 tcgctggcat cacactgggc ctggttctgg tctacctgtc attaatcgct gctgccttat
    18721 ggggatggag ccggttggac gccaggatgg agaaagtttt tctcttatcg accaaaggcg
    18781 aaaacccact catcgaatac gcggacaatc tacgtttcgg ggagcgggtg atttactggc
    18841 aaaccggctg gaatattttt aacgaccatc ccctcatcgg tgttggggtt ggtttttcgg
    18901 ggttttactt cccggaatat ctaccggatg cggggtgggg gttgagtgaa actcgcaaat
    18961 taatgttccg gtctgaaggc ttgttgaaca taaaaaacct gtggtcgcgg ctgctggctg
    19021 agacgggaat cattggtttc gcattgttcc tcacttttct ggtgctcttt gccttcacct
    19081 ctctggaaat ggtcgtaaag ggtgatggga gacgaaaaac attgggtttt atggggtttt
    19141 tcatgctggc tgccttggta attgaagaat ttagcgtgga cagtttcgcg ctgccctatt
    19201 tatggttcag tatggggttg gtcaccgcgg gttggcgttg gtacactttt aagaatggtg
    19261 gagaacatgg ataaatctga aaagttggtt ttactggttt ttctggtcat attagtctcg
    19321 gcttgctgcc tgctggtggc ttgcggcggg atggctcttc tgttatcaag cgtatcggga
    19381 ttcctttctc cgacagcaga cacggttaca ttcgcggatg gtacgccaac gccgggtagc
    19441 cccagctcag taatacaacc ttcgccaatg gcgacaaaat tatcttctga gggttttgag
    19501 aggatctacc acacgctctc cacgctggaa aacaccttca tcccaagcgc tgatttaatt
    19561 tacctggctg aacgctttga aggcaaaaaa ggcattcccc ggcagttgac cacacccccg
    19621 gtggcttacg aagtgggaga ccggctggat ttttacaaga tcaacacaga taccaacgtc
    19681 tccagtgtta ccactgccgt gttgcgctat gcttcagaca cattatattt ctgggttgaa
    19741 gagggtgtaa aaattgacca gaaagaattg cagaaaacgc tcgacgtctt tgaaagcaag
    19801 atctacccaa ccaaccagga attttttgga acagaatgga tcccgggggt tgataacgac
    19861 ccgcaccttt acattttata cgccggagat ttaggttatt ccctggcagg ctacaacgca
    19921 tcttcggatg gcgtactgcc actggcgtac aagaattcga atgcgcatga aatgttttat
    19981 atcaattcgg atgttcaaac cctttcagac ccctacaccc tgagcgtgat ggcgcatgaa
    20041 ttccaacatc tgattcatgg ctatcatgac cctaacgagg agttgtggat aaatgaaggc
    20101 ttctcggaac tggcaaccct gttgaatggg tacagcgcag gtgggtttga ttatctcttt
    20161 tcctacgaca cagatgtgca attgaacgac tggtcaacag aatctggaaa aaacgatgtg
    20221 catt
//
LOCUS       DAOSPM010000026        20122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2677_length_20122_cov_13.099367, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000026 DAOSPM010000000
VERSION     DAOSPM010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 20122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20122
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2677_length_20122_cov_13.099367"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..783
                     /locus_tag="PLW25_03840"
     CDS             139..783
                     /locus_tag="PLW25_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOS53654.1"
                     /translation="MLKVDFHTHTRYSEDSLTPIHELISAARKRGLDRVVVTDHNVLT
                     GALEAWRAAPDLVIPGEEVQTSEGELLAAFVTVVVPRGLEPLEALHRLRKQGAFISIS
                     HPFDPQRSGWSRETLDLIASQVDAIETGNGRVLTPAYNQRAAEYAASLGLPGTAGSDS
                     HHPSEVGRMYTLVPEFNDAESLRLAMRSARVCGEPSSPLVHLYSVQAKAVKRWR"
     gene            868..951
                     /locus_tag="PLW25_03845"
     tRNA            868..951
                     /locus_tag="PLW25_03845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:902..904,aa:Leu,seq:taa)
     gene            1216..3174
                     /locus_tag="PLW25_03850"
     CDS             1216..3174
                     /locus_tag="PLW25_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOS53655.1"
                     /translation="MPTLKKNSSIKELLRPVMRPAWSPTSLMVLTSAIAALYILMEWI
                     FLFTRPSFLQTIAFLQKFLALSTASGLLVLACGLALLPFLASGQVFQQAAFRKMLTLT
                     AMLLPAFLLAALALLLLDNFTYTIFQLGIVSTRDWIRGLYGVLFSFLVVYLFFKLVAF
                     HHLLAAFFSRWKPAQRLMLVGSIFLLSLASAGIPALSMGNQLLGSSRFHPAGSRSIPP
                     DILLITVDGVNAEYTSLDAYERDTTPFLRAISAESLRARNAFANAQGTIGSITTLLTG
                     KYPMDTRVIYSSDLLQGEDAYQHLPEILEDFGYYTVQLSNPVYADAYKTNFQNAFNEA
                     NGRSADQQPLAFALARIYPGINYLFQQEMTDRLSDRLGHIFFLSDMSNPYEQVTEAAV
                     KFNDWEKIDYLFRLLEESRQPVFAHLHWMGTHGPRYFPDKQVFSAGISVDEQGNNNQL
                     FYYDAILEFDQAFEEIYARLEKSNQLENTLLVVTSDHSQKWTNSRLPLLIRFPRAERQ
                     GVLDQNVSTVDIAPTLLDYLGIKTPKWMAGQSLLSPSYIERPIFTVRIPKSSKDPVTG
                     KVIYPESEPPFFQFGRISVIVCDQWYDLDLVEMSMTHGNITAYATPCSAQINESQALE
                     LIMQHLQRYDFDTDTLLPLRARLEGEHP"
     gene            complement(3215..5173)
                     /locus_tag="PLW25_03855"
     CDS             complement(3215..5173)
                     /locus_tag="PLW25_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOS53656.1"
                     /translation="MLPIEEPVVFFLVIMAIILVMPLLSERVHLPGIVGVIIGGMLIG
                     PHGFNILAANDRMEFLATIGLIYLMFSAGLEVDFHQFMRIKGKAAIFGLMTFVIPQVF
                     GMALGWMIHMPLLGCVLLGSAFASHTLLAFPILTRLGVTKNEAVAVTAGATILTDIGA
                     FIILAIVLGSNGGQLELGYFIKIFVLLVAFALATLFILPRLGKWFFQKVKGRTVEFQF
                     VIVLLFIAAMGAKLIGVHEVVGAFLAGLAVNATLPRHSPVTSHVLFMGESFFIPVFLL
                     FSGMVTDPLTLLHTPGALFLTLGILVVAYLSKFLASLVTSRLFKYSKEEFLTVFGLSH
                     AQAAVTIPTLVIGMQLNLFSTELFNAAMMMILLTSITSPLIVQKFAPGLHQEVTELEE
                     LPLFQRVLVPIANPGTQEHLLALAGLLAKSGGGEVIALNVVLDRNDQGANLSQQKELL
                     NRVPELLKDPETRYELVPRLADSYARGILQVSRERGSTLIVMGWRGKRTFRTSVLGTV
                     LDEVIWGSDTPVMVGKLSRSLNGTRRVLLLIPAGVVSPSALRRVVQANMTLARELNVP
                     LEVLAAKSYLELIKRIIGKNPNQIAITVQEMVGALRLKDLEKNVESDLLVIPGFGSRK
                     HFIASLGNLPERLADSFSGNLIILHFDR"
     gene            complement(5402..5548)
                     /locus_tag="PLW25_03860"
     CDS             complement(5402..5548)
                     /locus_tag="PLW25_03860"
                     /inference="COORDINATES: protein motif:HMM:NF039193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HOS53657.1"
                     /translation="MAVIGYCMKCKKKTEMKDTVEVTMKNERKAMKGKCSVCGTGMYK
                     ILGK"
     gene            5742..7673
                     /locus_tag="PLW25_03865"
     CDS             5742..7673
                     /locus_tag="PLW25_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOS53658.1"
                     /translation="MTTSSEKFHGRKAFTPTPLKRRTPVPSDIEIAQEATLKPIMLIA
                     EELGLLPAETEPYGDTKAKVRLEVLQRLKHIPDGKYIDVTAITPTPLGEGKSTTMVGL
                     SQALGAHLGKSVITCIRQPSQGPTFGIKGGAAGGGYSQVVPMEEFNLHLTGDIHAITA
                     ANNLLAAAIDARMFHEKEATDEQLFNRLLPKDKNGKRHFTRGMVARLEKLGISVSDPD
                     ELNEEQRRQLCRLDIDPDTITWRRVVDTSDRFLRGITIGEGPDEKGFTRRTGYDITVA
                     SEIMAILALTTSLKDMRERFGRIVIGISHGGDAVTAEDLGVAGAMTVLMRDAIKPTLM
                     QTVEGTPVFVHAGPFANIAHGNSSILADMIALKLADYVVTESGFGADMGMEKFMDIKC
                     RYSGLIPSVVVLVATIRALKMHGGGPKVVAGKPLDEAYTSENLELLKAGLPNMLHHIK
                     IARKYGIPVVVAVNRFVNDSPAELEMVRKSAVEEGGAEDAVVCEHWEKGGEGAVDLAR
                     AVIRAAEKPAKFEFLYPLDWSIKQKIECITREIYGAEGVDYLPEAEERIAEYTRLGFD
                     KLPICMAKTHLSLSHDATLKGVPKGVRIPIRDIRASVGAGFLYPLLGTMSTMPGLPTR
                     PIFMEIDIEPETGRIIGLS"
     gene            7894..8973
                     /locus_tag="PLW25_03870"
     CDS             7894..8973
                     /locus_tag="PLW25_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOS53659.1"
                     /translation="MRYYQIEDLKEFASRYFTRWGVSEEDAEITADVLVSADARGVDS
                     HGLIRLQNYYGSRLESGLTNPRAALTTLKETPTTLSLDAHNGLGHPAGVKAMRMCIEK
                     ARNAGLAMATVRNSNHYGIAGYYAMMPLSEGMIGISLTNSAPLVAPTHGRKAMLGTNP
                     IAVAVPAGGARPYVLDMATSIVPIGRITVYQKEGRSIPAGWGINSEGDVTEDPSRVVE
                     GGALMPLGGPELLRGYKGYGLALLVDIFAGVLAGAGFGERVLGPAVSGAANTGHFFAA
                     LRIDAFRDPADFAADMNTLLGALRDSPKAAGEERIYIHGEKEFEKADRALVEGVALSE
                     VAVKSLIQAGKQAGIPFDLVPLRID"
     gene            complement(8970..10586)
                     /locus_tag="PLW25_03875"
     CDS             complement(8970..10586)
                     /locus_tag="PLW25_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOS53660.1"
                     /translation="MEQSHLWWRDGVIYQIYPRSFADSNGDGIGDLIGITQRLDYLVE
                     LGVDAIWLSPINPSPDVDFGYDVADYRNIDPKHGTLADFDHLVKAAHERGLRIILDLV
                     LNHTSDQHPWFLESRSSLDNPRRDWYIWRGSPKGKREAPNQWQSVFGGKAWEFDEVTG
                     QYYYHMFCPEQPDLNWRNPEVYREMLDIFRFWAERGVDGFRLDVFNQYFVDAQFRDNP
                     RKCGIRPFDCQHHLYDTNQPEIQQVVADIRAVLDRYPERYAVGETFLPTTEMASSYSG
                     PGKLHATFDFTMLKSPWNPARYLQCVLRQEELLPEGGWPNYVLNNHDNSRTASRLRAG
                     RGDEKLKVSAAMLLTLRGTPFLYQGEEIGMRDIQVSRAELQDPVGKRYWPLPVGRDGF
                     RSPMQWNGEANAGFTTGKPWLKLHPGYKTRNVESQRKDPASLFNFYKSLLRLRRMYAA
                     LQRGMFLPLHHNPQRVLAYLRQDAAQTILVALNFARRRSKLALGGEITRHQWEMLLSN
                     KEKSQPSIDKGWLQLDGHEVCILLQKPGSG"
     gene            10846..>12146
                     /locus_tag="PLW25_03880"
     CDS             10846..>12146
                     /locus_tag="PLW25_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53661.1"
                     /translation="MTINRTVKAVCYILCAMFIASCSLLTSLSPSVHTDLTPGIDEPT
                     PVNNQAGLSPEENATQNDQDLVHLWAENSTLYPLEVYGVAVGAPEDTLGCGSEFLSGY
                     EEPEMEVTINLNYAQALIPQKVNLVLKEMNEGITRVEILNTRTGLGREIFRENLHRSF
                     SRLENQACALRLELPVEIDFEVNTVIIGFKNLAAAALLDAVELVGSPLSYEEPPVAWR
                     VALNGKPLSLTVDPLNQVWVSLEPNIILRYDVEGNLLEELPAIPSGHITDMAINTDGD
                     LVLNDEDFGEYIILSKGVEVARGGGDAPTLAVAVEPGKKNIYMLGSLDGLYYLLNYLP
                     NSFEINNPLPLVSTSYTSLAFNSANQLFTIRDQDGFLAEIDENSGLEFYSIPLSAYNQ
                     PGVLPKSMAIDKNDNFYVLYSSNPENAAVKVYDVDGFYFRS"
     assembly_gap    12147..12246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12292..13140
                     /locus_tag="PLW25_03885"
     CDS             12292..13140
                     /locus_tag="PLW25_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS53662.1"
                     /translation="MHRERVSDNVYWFQSEVYAQVTAGAVTGPNWAVVIDTLALPEEA
                     LEMRTFIEDNLGVPVRYVINTHYHADHSWGNCFFPGATVIAHSIARETMLEKGEPSLE
                     SIKKQNMAFKQVKLVLPHLTFSEGNVTLRVGKKSLVMFATPGHSADGISVLVEEDRVL
                     FAGDAFMPLPYMVDGDPEEISATIKRIGKMGLENIIQGHGDIILRGEIEEATRSNLAY
                     INAVRKAVRAAAKKRDPIEALAEIDIESCGKSRVTLGGLGVELHKRNVVYLYKTLAAE
                     ETNADQ"
     gene            complement(13165..13983)
                     /locus_tag="PLW25_03890"
     CDS             complement(13165..13983)
                     /locus_tag="PLW25_03890"
                     /inference="COORDINATES: protein motif:HMM:NF040134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53663.1"
                     /translation="MSGQKHLPDVERLSVVIALILLAYASTALISLPARSINLQLPGI
                     LLVVKVDFSTLVALVAALLAAAGVEWVLGVHPSLSVHQHWRHWHHWLVPAFSAVAIGI
                     TLGTLPVGAAWWIVFGLGGFLLAAVLTIEYISADPADARYSFAVLGLDAASFALFLII
                     ITAIAGADLRLYILLAAIVPVVFLISARSLFLRLHGSWRLGWAAGITLIIAQLAAALF
                     YLPLRPLQYGLLLLGLLYGLISLASNKEEKANPRTIWIEPTVVLLVFSLLGFLL"
     gene            14070..15098
                     /locus_tag="PLW25_03895"
     CDS             14070..15098
                     /locus_tag="PLW25_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53664.1"
                     /translation="MNTNEPMLVAAPPRIFPTILKGFNTVAGQVHLILLPVLLDLFLW
                     LGPRLRIYDLFAPAIQMLNANMLKVAPAEMLETVRNATALYTEMFQQFNLFSAIRTLP
                     VGVPSLVARMSVAQTPAVFFGNFEISSLRVGIATLGGLLTLGFLLGAIYFNALSRYSQ
                     PEVNAFNWNKLFNQYAQTLVFFLILVALLIAISIPLLLLVSILSLISAGLAQFIIVLV
                     GFLAIWLILPLVFSPHGIFALDQKVVPSMLLSLRMVRFFLPGTSFFILVCILINEGLN
                     MVWTIPGTASWLLLIGIGGHAFIVTALLAASFLYYRDGIKWMQYNVQKMTETLKKQES
                     GGTQDEQP"
     gene            15085..17007
                     /gene="gyrB"
                     /locus_tag="PLW25_03900"
     CDS             15085..17007
                     /gene="gyrB"
                     /locus_tag="PLW25_03900"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOS53665.1"
                     /translation="MNNHNPTNYGAKDIQVLEGLEAVRRRPGMYVGGTDFKALHHLVY
                     EVVDNSIDEALMGACNRIDIIIHPDSSVTVQDDGRGIPVDIHPEKQKSALEVVMTVLH
                     AGGKFGHSSYKVSGGLHGVGVSAVNALSEWCEVHVYRDGHEYFQRYERGIPQEAVKII
                     SKVPAHKTGTSTTFKFDREIFKGDVDYRFDTLAQRFREMAFVTRNVKIKLVDERPPEK
                     ETTFLFEGGIVSFVRYINRSRDVLHPVYYVEKEIDNIGIEIAVQFTDAYNESVFSFAN
                     TINTIDGGTHLTGLRTAITRVINDYARRANLLKDADANFTGDDTREGMTAIVSIKHPD
                     PQFESQTKVKLMNPEVQTYVQQVVGESFGAFLEENPSAGKAIVNKCLTSARARDAARK
                     ARDLVIRKSALESLTLPGKLADCSDKDSNRTEMYIVEGNSAGGSAKQGRDRHFQAILP
                     LRGKILNTERARLDKILGNEEIKSIISALGTGIGDNFDIKNLRYGKVIIMTDADVDGS
                     HIRTLLLTFFFRYMPQIIEEGHLYIAQPPLYRIAHKNQVQYTYSDAEKEEYLKSLGAQ
                     AEKATLQRYKGLGEMNPTQLWETTMNPESRTLLQVSIDDAASADHTFDMLMGSEVPPR
                     TRFISTHAHAVRNLDI"
     gene            17094..17927
                     /locus_tag="PLW25_03905"
     CDS             17094..17927
                     /locus_tag="PLW25_03905"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOS53666.1"
                     /translation="MREVVEIIEPQWVIEGAGVRLRRSIGSSSFDHLDPFLLLDHFGS
                     TEPADYLRGFPMHPHRGIETVTYMLAGLVRHKDSLGNKGEITSGDVQWMTAGRAILHE
                     EMPEDLGGQMEGFQLWVNLPAKLKMNPPRYQEIKAGKIPWLNPEKGVAVRLIAGKYAN
                     TTGPVTEIAVEPLYMDVAVDSGNSFTYQLTENHNAFLYVFRGEGEFCGVRTQATKLLV
                     LGKGESVSVKAGLLGTRFLLVAGKPLEEPIVRYGPIVMNTVEEIKQTLREIRDGTFIQ
                     R"
     gene            complement(18302..19507)
                     /locus_tag="PLW25_03910"
     CDS             complement(18302..19507)
                     /locus_tag="PLW25_03910"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOS53667.1"
                     /translation="MKQTNHLWVCVLMALMLLLTACAGGTAPTATQISLEEAVKQTIA
                     AMPTATNTPTPTATPQPTATPTQSIVRYGPDNFPPNVDPLTGLEVADPSILDRRPMMI
                     KVSNFPREGRPHAGLSAADIVFDYYTGEGGNRYLALFYGQDSNQIGPIRSGRLIDRYL
                     VGMYQGVLGAVYAFPSTWETILGYLGWSRVISEGTNTCPAMCRQENVRAEISVFADSA
                     EMTKYYAARPNASNTRQNLNGMAFNTLPPAGGVEARELTHQFGKNNYAGWKYNPETKK
                     YMRWIDSIKENGDVEMVPLTDRNTGEQLQFSNVLLIYAEIETLHHDDTLHEIHILNAN
                     GRAVVFRDGLKYEATYKSGWDTPIQFFNQDGSPFELQPGNTWMHFTGLHSELTEDPSG
                     VWLSRNWKP"
     gene            complement(19587..>20122)
                     /locus_tag="PLW25_03915"
     CDS             complement(19587..>20122)
                     /locus_tag="PLW25_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53668.1"
                     /translation="VVPPQEPWATSPANSSVYRISADGLSSELLFNTQMSFRPMEIAA
                     ISPDLSKLAFLVPYGSLTDNLYTLRLANIDGSGLTDYTTGKINRIPVWSPDNNKFFYR
                     DDASGAWIGQPGSAPVSIPDFNNTRDLVWIDGNRYIGASGPEGGWKLLLGTLGSPTGV
                     IYSSTSGGENLQFTVNR"
BASE COUNT         4754 a         5251 c         5440 g         4577 t
ORIGIN      
        1 tggggtttca acccgaactt gccgttactg ccaccatcct tttccgtatg tctacctact
       61 ggattagcct gctctttggt atactcttct ggtctgccgc agcgaaaccc tgcacggcgg
      121 aaccaggagt gagcggattt gctgaaagtt gattttcaca cacacacacg ctattctgaa
      181 gacagcctta cccccattca tgaactaatt tccgccgccc gaaaacgcgg gttggaccgg
      241 gtggtggtaa ctgaccacaa tgtactgaca ggcgcgctgg aagcctggcg cgcggcgcct
      301 gacctggtga ttcccggcga agaagtgcaa accagcgagg gcgagttgct ggctgctttc
      361 gtaacggtcg ttgttccgcg cgggctcgag ccgctggagg cgctgcaccg cttacgcaaa
      421 cagggcgcgt tcatcagcat ctcccatccc tttgacccgc agcgctcagg ctggagccgt
      481 gaaaccctgg acctcatcgc ctcgcaggta gacgctatcg agaccggtaa cggccgtgtg
      541 ctcacccccg cgtacaacca acgggctgcc gaatacgcgg cgagccttgg actgcccggt
      601 accgcgggtt cagactctca ccatcccagc gaggtggggc gcatgtacac gctcgttccc
      661 gaattcaacg atgctgaaag cctgcgcctg gctatgcgtt cagcgcgggt gtgcggggaa
      721 ccctcttcac cgctggttca cctttactcg gtacaggcga aagcggtcaa gagatggaga
      781 taactggcgc atggcaacgg gaaccggcac ctggcaggcg atttttcact ccgatttttg
      841 tgaattgcga taaaatagaa actgtttggg gcgatggcgg aatcggcaga cgcaacggac
      901 ttaaaatccg tcgatggtga catcgtgagg gttcgacccc ctctcgcccc acttccaact
      961 gtaacagcaa tccaaagggc agcgcttcct gctctccgaa gaaccttcct ccaatggagg
     1021 tttggcggct ggtgaatacg agctatgaag aaaaactatt tttttgatgg ctcaagcgcg
     1081 cccgctgatt tgcgttggtc ttcgcgaacc ttccgcacaa tttactttgg cgcctctgga
     1141 ccttcctcgt ttggttttcc cctgcgtgta ccttaatggt ttaaaattag ccccgtcaaa
     1201 aacggatagg tgttgatgcc aaccctcaag aaaaactcat ccatcaaaga actgctgcgc
     1261 ccggtcatgc gccccgcctg gtcaccgacc agcctgatgg tgctcaccag cgccatcgcc
     1321 gcgctttata tcttgatgga atggattttc cttttcacgc gcccttcttt tctacagact
     1381 attgctttcc tccaaaaatt cctggcgcta tccacggctt ctggtttact ggtgctggcc
     1441 tgcggcctgg ctctgttgcc tttcctggcg agcgggcagg ttttccagca ggctgcattc
     1501 agaaaaatgc tcaccttaac cgccatgctg ctgcctgcat tcctgctggc tgccctggct
     1561 ttactcctgc tggataactt cacttacact attttccaat taggcattgt ttcgaccagg
     1621 gactggatcc gcgggctgta tggggtgctg ttttcctttc tggtcgttta cctgttcttc
     1681 aagctggtgg cctttcacca ccttctggct gcgttctttt cacgctggaa gcctgctcaa
     1741 cggctaatgc ttgtgggtag tatcttcctc ctcagcctgg ccagcgccgg catccccgcg
     1801 ctgtccatgg gcaatcaact attgggttcc agccgcttcc accctgcggg gagccgctcc
     1861 atcccccctg atatcctgtt gatcacggtg gacggcgtga acgctgaata cacttctctt
     1921 gatgcctatg aaagagacac cactcctttt ctacgggcga tttctgccga atcgttaagg
     1981 gcacggaacg catttgccaa cgcgcaggga accattggtt caattaccac cctgctgacc
     2041 ggcaaatacc ccatggatac gcgagtgatc tattcttctg acctgttaca gggagaagat
     2101 gcctatcagc acctgccgga aatcctcgag gacttcggct attacacggt tcagctcagc
     2161 aacccggttt acgctgatgc ctataaaacc aacttccaga acgcttttaa tgaagccaac
     2221 ggccgttctg ctgatcaaca accgctggcg tttgcccttg ccaggatcta cccgggaatc
     2281 aactatctat ttcaacagga gatgaccgac aggctcagtg acaggctggg tcatatcttt
     2341 ttcctttcgg atatgtctaa cccctacgaa caggtcaccg aagccgcggt gaagttcaat
     2401 gactgggaaa aaatcgatta tctattccgc cttcttgaag aaagccgcca gccggttttt
     2461 gcgcacctgc actggatggg cacgcatgga ccgcgctatt ttcctgataa gcaggtgttt
     2521 tcggcgggca taagtgttga tgaacaggga aacaacaacc agttgttcta ctatgacgcg
     2581 attctggagt ttgaccaggc gtttgaagag atttacgcgc gccttgagaa aagcaatcag
     2641 ctggagaata ccctgctggt ggtcaccagc gatcacagcc agaaatggac caattccagg
     2701 ctgccgttat tgatacgctt ccctcgcgct gaacgccagg gcgttcttga ccagaacgtc
     2761 agcactgtgg atatcgcccc cacgctgctg gattaccttg gcatcaaaac gcccaaatgg
     2821 atggcggggc aatctttgct atctccttct tatattgagc gcccaatctt tactgtcagg
     2881 atcccgaaat catccaaaga ccctgtcact ggcaaggtga tttatcctga gtcagaaccg
     2941 cctttttttc agtttgggag gatttcagtc attgtctgtg atcaatggta tgacctggac
     3001 ctggtggaaa tgagtatgac ccacggaaat ataacagcat acgcaacacc ctgttcagcg
     3061 cagatcaatg aatcacaggc gctagaactg attatgcaac atctgcaacg ctatgatttt
     3121 gataccgata cgttgctgcc attaagggcc aggctcgaag gtgaacaccc ataatctatt
     3181 ttccgctcag cccaaaaaat gctggcaaac ctgttcagcg gtcaaagtgc aaaataatca
     3241 ggttgccgga gaatgaatct gccagacgtt ctggcagatt gcccaggctg gcgatgaagt
     3301 gcttgcgcga cccaaaccct ggaatgacca gcaggtcact ctccacgttt ttctcaaggt
     3361 cttttaagcg taaagccccg accatttctt gaaccgtgat ggcaatttgg ttaggatttt
     3421 tcccgataat gcgtttaatc aactccaggt aactcttcgc ggccagcact tccagcggca
     3481 cgttcagttc tcgcgccagc gtcatgttgg cctgcaccac ccggcgcagg gcagacgggg
     3541 acaccacgcc cgccgggatg agtaacagga ccctgcgcgt gccattaagt gagcggctca
     3601 gttttcctac catcacagga gtgtcggagc cccaaatcac ctcgtccaac accgtgccta
     3661 atacgctggt tctaaaagtg cgtttaccac gccagcccat cacgatcaag gtggaaccgc
     3721 gctcgcgcga gacctgcagg atgcccctgg cgtaagaatc agccaggcgc ggaactaatt
     3781 cgtacctggt ttcagggtct tttaaaagct ccggcacccg gttcagaagc tctttttgct
     3841 gcgagagatt ggcgccttgg tcgttacggt ccagcaccac attgagcgcg atcacctcac
     3901 cgccgccgct cttggctaac agccctgcta aagccaacag atgttcctgc gtgcctgggt
     3961 tggcgatggg taccagcacg cgttggaaca agggcagctc ttccagttcg gtaacttcct
     4021 ggtgcagccc tggagcaaat ttttgcacaa tcagagggga ggtgatggag gtgagcagaa
     4081 tcatcatcat tgctgcatta aaaagttccg ttgaaaacag gttcagctgc atgccaatca
     4141 ccaatgtcgg gatggtaaca gcagcctgcg cgtgagacaa cccaaacacg gtgaggaatt
     4201 cttctttact gtatttgaat aacctggagg tgaccagcga agccaggaat ttggaaagat
     4261 aagccacgac caggatgccg agcgtcagaa aaagcgctcc aggtgtatgc agtaaggtta
     4321 acggatcggt gaccatgccg ctgaagagta aaaaaacggg aataaaaaag gattcgccca
     4381 tgaataggac atggctggtg acagggctgt gccgcggaag cgtggcgttg accgccagcc
     4441 cggctagaaa ggctcctact acctcatgca ccccaatcag cttggctccc atggcagcga
     4501 tgaaaagcaa cacgatgacg aattgaaatt caaccgtgcg ccctttgacc ttctggaaga
     4561 accatttccc cagcctggga aggataaaga gagtggcgag ggcgaaagcc acaagcaaga
     4621 cgaagatttt gatgaaataa ccaagctcca gctgcccgcc gtttgaaccc aagacaattg
     4681 ccagaatgat gaaggcgccg atatcggtga gaatggtagc cccggcggtt accgccacgg
     4741 cttcgttttt ggtcaccccc agcctggtaa gaatgggaaa agccagcagc gtgtgagaag
     4801 caaaagctga gccaagaagg acacaaccga gcaagggcat gtggatcatc caccccaacg
     4861 ccatgccaaa cacctggggg atgacaaagg tcatcaaacc gaagatggcc gctttccctt
     4921 ttatgcgcat gaactggtga aaatccactt ccaaaccagc gctgaacata aggtagatca
     4981 gcccgatggt ggcaagaaat tccatacggt cgtttgccgc cagtatgttg aacccgtgcg
     5041 gcccaatcag catgccgccg atgatcaccc ctacgatccc gggtaagtga acccgctcgg
     5101 aaagcagcgg catgaccagg atgatcgcca tgatgaccag gaaaaagacg acaggctctt
     5161 caatgggcaa catcagaagt atcctctcct gggtcagcct gtttttgtgc gaactgaaag
     5221 attttttttc tcatctggag cgctattcgc ccgcggtgta tgattggatc atccctttgg
     5281 cttatgagta gtttaacgtt acgatttgag ggcaatcatg gatggcgagc agctggaatc
     5341 tgccgtaacg aaaaccgcac cagagtttag gcgcggtttt tcatggtaga aagaatccgg
     5401 cttatttacc gaggattttg tacataccgg tgccgcagac tgagcacttt cctttcatcg
     5461 ctttgcgctc gttcttcatg gtcacttcca cggtgtcttt catttcggtt ttctttttac
     5521 atttcatgca atatccgatt actgccattt ttacctcctt tggttttggc gacgattgaa
     5581 ttagaacata tctaatctga aattattata gcaggtactt atctgaatag ttcaaccagt
     5641 tttcatgaag aacaaggtgg tttaccagga ttttttcgct tgcatttcct ctctatttcg
     5701 tctttaatta acctgattag atcaattgtc aaggagaaaa aatgacaacc tcttctgaga
     5761 aattccacgg tcgcaaagcc ttcactccaa ccccgctgaa acgacgcacg cccgtaccat
     5821 ccgatatcga aatcgcccaa gaggcgactt taaaaccaat catgttaatt gcggaggagt
     5881 tgggtcttct gcctgctgaa acagaacctt acggcgatac caaggctaag gtgcgccttg
     5941 aagtgctgca acggctaaag cacataccgg acgggaagta cattgatgta acggccatca
     6001 cgccgacgcc gttgggggaa ggcaaatcca ccaccatggt ggggctcagt caggcgttgg
     6061 gggcgcacct tggcaagagc gtgatcacct gcatccgcca gccttcacag gggccgacgt
     6121 tcgggatcaa aggcggtgca gccggcggtg gttactcaca ggttgtaccg atggaagaat
     6181 tcaacctgca tctcacgggc gatatccatg ccatcaccgc tgccaacaac ctgttggcgg
     6241 cagcgatcga cgcgcgcatg ttccacgaga aggaagctac agacgagcag ctgttcaacc
     6301 gcctgctgcc aaaagataag aacggaaaac gccactttac caggggaatg gtcgccaggc
     6361 ttgaaaagct gggcatcagc gtttcagacc cggatgaact gaacgaggag caaaggcggc
     6421 aactctgccg cctggatatt gaccctgata ccatcacctg gcgccgcgtg gtggatactt
     6481 ctgatcgttt cctgcgcggc attaccattg gcgaaggacc ggatgaaaaa ggtttcaccc
     6541 gccgcacagg ttacgatatc acggtggcat cagagatcat ggccatccta gccctcacca
     6601 caagcctgaa agacatgcgc gaacgctttg gacggattgt cattggcatc agccatggcg
     6661 gagacgcggt cacggccgaa gacctgggcg tggccggggc gatgacggtg ctcatgcgcg
     6721 atgcgattaa gcctaccctg atgcaaaccg tggaaggcac cccggtcttt gtgcacgccg
     6781 gaccctttgc caatatcgcg catggaaatt cttctatact cgcggatatg atcgccttga
     6841 aactggctga ttacgtggtg accgaaagcg gttttggcgc ggacatgggc atggaaaagt
     6901 ttatggatat taaatgtcgc tattccggcc tgatcccttc cgtggtggtg ctggtagcca
     6961 cgattcgcgc gctcaagatg cacggaggtg gacccaaggt ggtggcagga aagccattgg
     7021 atgaggctta cacctctgaa aacctggaat tgttgaaggc tggtctgcca aacatgttgc
     7081 accatataaa aatcgcgcgc aaatacggca ttcccgtggt ggtggcggtc aatcgttttg
     7141 tcaatgacag cccggctgaa cttgaaatgg tgcgcaaatc cgcagtggag gagggcggtg
     7201 cggaagacgc ggtggtctgc gagcattggg aaaagggagg ggaaggcgcg gtcgacctgg
     7261 ccagggcggt catccgggcg gccgagaaac cagccaaatt tgagtttctc tacccgctgg
     7321 attggtccat caagcagaaa atcgaatgca ttacccgcga gatctatggc gcggaaggag
     7381 tagattacct gcccgaagcc gaggaacgta tcgctgaata cacccgactg gggtttgaca
     7441 aactgcccat ctgcatggcc aagacccacc tcagcctttc acatgacgcc actcttaaag
     7501 gcgtgcccaa aggcgttcgt atccccatcc gcgatatccg cgcctcggta ggcgccgggt
     7561 tcctttaccc gctgctgggc actatgtcca ccatgccagg gctgccgacc cgcccgatat
     7621 tcatggagat tgatattgag cctgaaacgg gcagaatcat cgggttgagt taggcgagaa
     7681 tctcgccctg atgccactta ctcattgatt acgcctgcat tctgacgaag gctaaacgtt
     7741 cacttctggc tccagtttgc gctggagcca gttttcaatt gagcgaacag cgacaagaga
     7801 aaaggatttt gtgattcacc aaggcgggta tgcattggct cgccggacaa tcaagtataa
     7861 tgacctctcc acttcaacat tggagaaaac cagatgcgtt attatcagat tgaggatttg
     7921 aaagaattcg ctagccgata tttcacccgc tggggtgttt cagaggaaga cgccgaaatc
     7981 accgcggatg tgctggtcag cgcggacgcg cgcggcgtgg attctcatgg ccttattcgc
     8041 ctgcaaaact attatggttc ccgcctggaa agtgggctga ccaacccgcg agcagcgctg
     8101 acaactctga aagaaacgcc aaccacattg tcgttggatg cgcacaacgg gcttgggcat
     8161 ccggctggtg tgaaggcgat gaggatgtgc atcgaaaaag cgcgcaatgc agggttggcc
     8221 atggcaactg tgcgcaacag caaccattac ggtatcgcag gttattatgc catgatgccg
     8281 ctttcagaag gcatgatcgg catcagcctt accaactccg ctccgctggt agcccccacg
     8341 catgggcgaa aggcaatgtt gggcaccaat ccaatcgcgg tggctgtgcc ggctggcggc
     8401 gcccgccctt acgtgttgga tatggcaacc agcatcgtgc ccattgggcg tatcaccgtc
     8461 tatcaaaaag aaggcaggtc aatccctgcg ggttggggga ttaactccga aggagatgtt
     8521 acagaagacc cctcccgtgt ggtcgaaggc ggcgcgctga tgccgctggg cggcccggaa
     8581 ttattgcgcg gatataaagg ttatgggttg gcgctgctgg tggatatctt tgcaggggtg
     8641 ctggcaggag ctggttttgg cgagcgcgtt ttggggccgg ctgtttctgg cgctgccaat
     8701 accggtcatt tcttcgctgc cttgcgcatc gacgcttttc gcgatcctgc ggactttgcc
     8761 gctgatatga acacgctgct tggtgcgctg cgagattccc cgaaagccgc cggcgaagag
     8821 cgcatctata tccacgggga gaaagaattt gaaaaagcag atcgcgcact tgttgaagga
     8881 gtggcgcttt ctgaggtagc ggtgaaatcg cttattcagg ctggaaaaca ggcgggcatt
     8941 ccttttgacc ttgtgccgct taggattgac taacccgacc ctggcttttg cagcaagata
     9001 caaacctcat gtccatccaa ttgaagccag cctttatcaa tcgaaggctg gcttttttct
     9061 ttgttcgaaa gcagcatctc ccattgatgc ctggttattt caccacccag cgccagtttt
     9121 gagcgccttc tggcaaaatt aagcgccacc aagatggttt gtgcggcatc ctggcgcaga
     9181 taagccagca cccgctgcgg gttgtgatgc agcggtaaga acatcccccg ctgcaacgca
     9241 gcatacatgc gccgtaacct gagcagagat ttgtagaagt tgaacaaaga cgcggggtct
     9301 ttccgctgcg attcaacatt acgggtttta tacccggggt gtaacttcaa ccagggcttg
     9361 ccggtggtaa agcctgcgtt cgcttctccg ttccattgca tgggagaacg aaagccatcc
     9421 ctgcctactg gcaggggcca gtagcgcttt cctaccgggt cctgtagctc ggcgcggctg
     9481 acctggatat cacgcatgcc gatttcttca ccctggtaga gaaacggtgt cccgcgcagt
     9541 gtgagcagca tcgccgccga gactttgagt ttttcatcac cgcgcccagc cctcaagcgg
     9601 gaggcagtcc tggaattatc gtgattattt aaaacatagt ttggccagcc gccctctgga
     9661 agaagctctt cctgccgcag aacgcactgc aagtagcgcg ccgggttcca tgggctcttc
     9721 aacatggtga agtcgaatgt ggcgtgcagt ttgcccggtc cactataact tgaagccatc
     9781 tcagtggtgg gtaaaaaggt ttcgcctacc gcgtaacgtt ccgggtaccg gtccagcaca
     9841 gcgcggatat ctgcaaccac ctgttgtatt tccggctggt tggtatcata caggtgatgt
     9901 tggcagtcaa aggggcggat accgcatttc cgcgggttgt cgcggaattg agcgtcgacg
     9961 aaatactggt tgaacacgtc cagcctgaag ccatcaacac cgcgctcagc ccaaaaacgg
    10021 aaaatatcca gcatctcgcg gtaaacttct ggattgcgcc agttcaggtc gggctgttct
    10081 gggcagaaca tgtgatagta atactgaccg gtgacctcat caaactccca cgccttgccc
    10141 ccaaagacgg actgccattg attgggcgct tcccgtttgc ccttcgggct cccgcgccat
    10201 atataccaat cacgcctggg gttgtcgagt gaggagcgtg attccaggaa ccaggggtgc
    10261 tggtcggatg tgtgattcaa gacgaggtca agaatgatac gcaaccctcg ttcatgtgcg
    10321 gctttcacca ggtggtcaaa atccgccagt gtaccgtgtt tggggtcgat atttcgataa
    10381 tccgccacat cgtaaccaaa atccacatca ggcgaggggt tgatgggaga aagccagata
    10441 gcatccacgc ctaactcaac caggtaatcc agacgttggg ttataccaat caggtctcca
    10501 atcccatcac cattactgtc cgcgaatgaa cgcgggtaga tctggtagat cacgccatcc
    10561 cgccaccata aatgagactg ttccatgtgc atctccttgt gagttgattt taacatagcc
    10621 ccgttaatca acaccaacaa cgccgaacca gccagaccgg ctgcggaaag cgacgtattg
    10681 acctatggat agtgagccct cgcgcaggca gccgatgctc ttatgctgcc tgcctgatgt
    10741 gcgggttggt tcgtcatcac gcaagaccag agtaaggtgg ttgttttcct ggatgaacta
    10801 ttatatagta agggaacctg atgaaatttt gttgaaggag taaagatgac tatcaaccgc
    10861 actgtaaagg cagtttgtta catcctgtgc gcaatgttta tcgcgtcttg ctcgttgttg
    10921 acatccctat ccccatctgt acacacggac ttgacaccgg gcattgacga acccactccg
    10981 gtgaacaacc aggctgggct cagccctgaa gaaaacgcca cgcaaaatga ccaagacctg
    11041 gtgcaccttt gggctgaaaa cagcacgctc tacccgctgg aggtgtatgg cgttgcggtt
    11101 ggagcgcctg aagacacttt gggctgcggc agcgagttcc tgtcggggta tgaagaacca
    11161 gagatggaag tcacgatcaa cctgaactac gcgcaggcct tgatcccgca aaaggtcaat
    11221 ctggtgctca aagaaatgaa cgagggtatc acacgggtgg aaattctgaa cacgcgcacc
    11281 ggccttggca gggaaatttt ccgtgaaaac ctgcaccgct ccttctcccg ccttgaaaat
    11341 caagcgtgcg ccttacgcct ggagctgcca gtggaaatcg attttgaagt gaacaccgtt
    11401 atcatcggtt ttaaaaacct ggctgccgca gcgttgctgg acgcggtcga attggtggga
    11461 tctccgctct cttacgaaga gccaccggtt gcctggcggg ttgcccttaa tggcaagccg
    11521 ctcagcctga ccgtcgaccc gttgaaccag gtttgggttt cgctcgaacc gaacatcatc
    11581 ctgagatatg atgttgaagg caacttgctg gaagaactcc ccgccattcc aagcggtcat
    11641 atcacagaca tggccattaa tactgatggt gacctggtgc tcaatgatga agattttggc
    11701 gagtacatca tcctttcaaa gggagtcgaa gtcgctcgcg gcggtggcga tgctcccacc
    11761 ctggcggttg ctgtggaacc cgggaagaaa aatatctaca tgctgggcag cctggatggg
    11821 ctgtactacc tgcttaatta ccttcccaat agttttgaaa tcaacaaccc gctgccgttg
    11881 gtgagcacct cttacacgtc gttggcgttt aattcggcca accagttatt tacgattcgt
    11941 gaccaggatg gctttctagc ggagattgat gaaaactctg gcctcgagtt ttacagcatc
    12001 ccgctttcgg catataacca gcctggggtg ctgccaaaga gcatggccat cgataaaaat
    12061 gataattttt atgtgctcta ttcatccaac ccggagaatg ccgcggtgaa ggtttacgat
    12121 gtggatgggt tttactttcg ctcgttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnaatt gaatattgaa ttagcactac gaaggcatta aggaagagag catgcatcga
    12301 gaacgggtat cagataatgt ttattggttt caaagcgagg tttatgccca ggtaactgcc
    12361 ggggcggtca ccggtcctaa ctgggcggtg gtcatcgaca cgctggcttt acccgaagaa
    12421 gcgctggaaa tgcgcacttt catcgaggat aacctcggcg tgccggtacg ctatgtgatc
    12481 aatacgcatt atcacgccga tcattcgtgg ggcaactgct tcttccccgg cgccacggtg
    12541 atcgcgcatt caatcgcccg cgaaacgatg ctggagaaag gcgagccatc actggaatcg
    12601 attaaaaaac agaatatggc ctttaagcag gtcaaacttg tactgccaca tctgaccttc
    12661 tctgaaggga acgtaacctt gcgcgtgggg aagaagtcac tggtaatgtt tgccactccg
    12721 gggcactcag cagatggcat ttctgtgctg gtagaggaag acagggtgct ctttgccgga
    12781 gacgccttca tgccgctgcc ttatatggtg gatggcgacc cggaagagat ttctgccacg
    12841 atcaagcgca ttggcaagat gggtttggaa aatattatcc aggggcatgg agacatcatc
    12901 ttgcgcggtg aaattgaaga agcgacccga tctaacctgg cttacatcaa cgcggtgcgt
    12961 aaggcggttc gcgcggctgc aaaaaaacgc gacccaatcg aggcattggc ggagattgat
    13021 attgaatcct gcgggaagag ccgcgtcacc ctgggcggtt tgggtgtaga actgcacaaa
    13081 cgcaatgttg tctatttgta caagacgctg gctgctgaag aaaccaacgc ggaccagtag
    13141 cccgctggcg aaaagccggt ttttttatag taaaaaaccc aacagcgaaa agaccagcaa
    13201 caccactgtg ggttcgatcc atatcgtccg cgggttggct ttttcttcct tattggatgc
    13261 caggctgata agcccataga gcaaacctaa caatagcaga ccgtattgca aggggcgcag
    13321 cgggaggtag aacagtgccg ccgctaactg ggcgataatg agggtgatgc cggctgccca
    13381 gcccaggcgc caggagccat ggaggcgtaa aaataatgaa cgggcgctga taaggaaaac
    13441 caccggtacg atggctgcca gcagaatata caggcgcagg tcagccccag cgatggcagt
    13501 gatgataatc aggaatagcg cgaaactagc cgcatcgaga cccagcacag cgaaagagta
    13561 acgcgcatca gccgggtcag cggagatata ctcaatggtc aacacagccg ccagcaaaaa
    13621 cccgcccaaa ccgaacacga tccaccaggc agctcccacc gggagtgtgc cgagcgtgat
    13681 gccaatggct acagcgctga acgccggcac cagccaatgg tgccagtgcc gccaatgttg
    13741 gtgaacgctc aagctgggat gaacccccag cacccattcc accccggcag ccgccagcaa
    13801 ggctgccaca agcgccacaa gtgtagaaaa atcgactttg actaccagta aaatacccgg
    13861 aagctgtaaa ttgattgacc gcgcaggtag cgaaattaat gcggtggagg cgtacgccag
    13921 caaaatcaag gcgatgacca cacttaaacg ctcaacatcc gggagatgct tctgtccgct
    13981 cacaagggta ttttacccca tctactgtgc gccaatcgga gcgcgacgag cgggagaaga
    14041 ggggcagata tggtaaaatg atagactgta tgaataccaa cgaacctatg cttgtcgccg
    14101 cccctccccg gatattccct acaatcctaa aaggtttcaa cacggttgcc gggcaggtgc
    14161 acctgatatt attgcccgtt ttactggacc ttttcctgtg gctgggccca cggttgcgta
    14221 tctacgacct gttcgcgccg gcaattcaga tgctcaacgc taacatgcta aaagtggctc
    14281 ctgcagaaat gctcgaaaca gtgcgcaacg caactgccct gtacacggag atgttccagc
    14341 agttcaattt gttttctgcc atccgcacac tgccagttgg tgtgcccagc ctggtggcac
    14401 gtatgagcgt ggcccagacc ccggctgtct tttttggaaa tttcgagatt tcatcgctgc
    14461 gagtggggat tgcaacactg ggcggattac tcacactggg ttttcttctt ggcgccattt
    14521 actttaacgc gctctcccgt tattcccagc cggaagtgaa cgcattcaac tggaacaagc
    14581 tattcaacca gtacgcccag acactggtgt tctttttgat cttggtcgcg ttgttaatcg
    14641 caatttcgat acccttgtta ttgttggttt ccattctttc attgatcagc gccggactgg
    14701 cgcagttcat catcgtgctg gttggttttt tggcgatatg gctgatactg ccgctggtgt
    14761 tttctcctca cggcattttc gcgctggatc agaaagtggt tccttcgatg ctgctcagcc
    14821 tgaggatggt gcggttcttt ctccctggca ccagtttttt catcctggta tgcatcctta
    14881 tcaatgaagg actgaatatg gtttggacga taccaggtac cgcgtcttgg ctgctgctga
    14941 ttgggatcgg tggtcacgcg ttcatcgtaa ccgctttgct ggcggcgagt ttcctgtatt
    15001 atcgggatgg gattaagtgg atgcaataca acgttcaaaa gatgacagaa acgcttaaga
    15061 aacaggaaag cggaggaaca caggatgaac aaccataacc ccaccaacta cggggcgaaa
    15121 gacatccagg tgctggaggg gcttgaggcc gtgcgccgcc gcccagggat gtatgtgggc
    15181 ggcaccgatt tcaaagcctt acatcacctt gtatacgaag ttgtcgacaa ctccattgat
    15241 gaagccctga tgggcgcctg caatcgtatt gatatcatta ttcaccctga cagcagtgtt
    15301 actgtgcaag acgacgggcg cggcatcccg gtggatattc accccgaaaa acagaaatct
    15361 gcgctggaag tggtgatgac cgtgctgcac gccgggggta agtttgggca ttcttcgtac
    15421 aaggtttcgg gcgggttgca tggtgttggg gtttcggctg tgaacgcctt atccgaatgg
    15481 tgcgaagtgc atgtataccg ggatgggcat gagtattttc agcgttatga acgcgggatt
    15541 ccgcaggagg cggtgaagat catcagcaaa gtgccagcgc ataaaaccgg cacctcaacc
    15601 acattcaagt ttgaccggga aatcttcaag ggagatgtgg actaccgctt cgacaccctg
    15661 gcgcagcgct tccgcgagat ggctttcgtc acccgcaacg tcaagattaa gctggtcgac
    15721 gagcgccccc cggagaaaga aaccactttt ctttttgaag gcggcattgt ttctttcgtg
    15781 cgctacatca accgttcccg ggatgtgctc cacccggtct actatgtgga aaaagagatt
    15841 gataatatcg gtattgaaat cgcagtgcag ttcaccgatg cgtacaacga atcggttttt
    15901 tcctttgcca atacgattaa taccattgac ggcggtacac acctcaccgg cttgcggacc
    15961 gcgattacca gggtgatcaa tgattacgct cgccgcgcaa atctgttgaa agacgcggat
    16021 gccaatttca ccggtgacga tacccgtgaa gggatgacgg cgattgtcag catcaaacac
    16081 cctgaccctc agtttgaaag tcagaccaag gttaaattga tgaacccgga ggtgcaaacc
    16141 tacgtgcagc aggttgtggg agaatctttc ggcgcttttc tggaagaaaa tccctccgcg
    16201 ggcaaggcga ttgtgaacaa atgcctgacc tccgctcgcg cccgcgatgc cgcgcgcaaa
    16261 gcgcgtgacc tggtcatccg taaatcggcg ctggaatcgc tcaccctccc aggcaagctg
    16321 gcagattgct ctgataagga tagcaatcgc acggagatgt acatcgtgga aggcaattca
    16381 gccggcggtt cagccaagca ggggcgagac cgccatttcc aggctatctt accgctgcgc
    16441 ggtaagatcc tcaacaccga gcgcgcgcgc ctggataaga tactgggcaa cgaagagatc
    16501 aagtcgatca tctcggcgtt gggcaccgga attggtgata attttgatat caaaaatctg
    16561 cgttatggca aggtgatcat catgaccgac gcggatgttg acggcagcca tatccgcacc
    16621 ctgttgctga cattcttctt ccgctatatg ccgcaaatca tcgaagaagg gcatctgtac
    16681 atcgcgcaac cgccgctata ccgcatcgcg cacaagaacc aggtgcagta cacctacagc
    16741 gatgctgaaa aagaagaata cctgaaatct ttgggagcgc aggcggaaaa ggccacgtta
    16801 cagcgttaca aaggactggg tgaaatgaac cccacccagt tgtgggaaac cacgatgaac
    16861 ccggaatcgc gcaccttgct gcaggtttca atcgatgatg ccgcgtcagc agaccacaca
    16921 tttgatatgc tgatgggttc ggaggtccct ccgcgcacgc gctttatctc aacgcacgcg
    16981 cacgctgtgc gcaacctgga tatctgataa cctggctccc gacagacaaa caccgggagc
    17041 cttttattac agacgaatga aaggtggtca aagaagaaag gcaggagcaa ttgatgagag
    17101 aagtggtgga aatcattgaa cctcaatggg tgatcgaagg cgcgggcgtc aggctgcgtc
    17161 gctccatcgg gtcttcctcc ttcgaccacc tggatccttt tttgctgctg gaccattttg
    17221 gttcgactga accggctgac tatttgcgcg gttttcccat gcatccgcac cgtggaattg
    17281 aaacggtcac ttacatgctg gctggcctgg taaggcataa agacagcctg ggaaacaagg
    17341 gcgaaatcac ctcgggtgat gtacagtgga tgaccgcggg gcgcgccata ttgcatgaag
    17401 agatgccgga ggacctgggg gggcagatgg aagggtttca gttatgggtc aatcttccgg
    17461 cgaagctgaa aatgaacccg ccgcgatacc aggaaatcaa ggctgggaag atcccctggc
    17521 tgaaccctga aaagggcgtg gctgttcgac tgatcgctgg aaaatatgcc aacaccacag
    17581 gcccagttac tgagatcgcg gttgaacccc tttatatgga tgtggcggta gacagcggga
    17641 attcctttac ctatcaactg acggagaacc acaacgcttt tctttacgtg ttcagaggtg
    17701 aaggtgagtt ttgcggggtg cgtacccagg cgacaaaact gctggtgttg ggcaaaggag
    17761 aaagcgtttc agtgaaagcc gggttgctgg gaacccggtt cctgctggtg gctggtaaac
    17821 ctctggaaga gcctatcgtg cgttatgggc caattgtgat gaacacagtc gaggaaatca
    17881 agcaaacctt gcgggagata cgcgacggga cttttatcca gcgctaagcc tgcggagttg
    17941 aaaaatgata tgggggtatt tttcactttt caagccttca ttgggctgaa cgtggttcct
    18001 tcgcgcaaaa cgtttccgaa aagagcattg ggctaagctg ggtcgaatcg cgaggttcag
    18061 gacactgccc ttcttcttat cggcatcgtt gggaggtctc cattgaggag gaagccacga
    18121 aagcatggag gggttgaagg cgcgcagggc gcacagggaa gacctggcga cagtcgtggg
    18181 tatgatgaag gatagattct gtttaccagt gtgaagccgc gcgcagttca cctggaatcc
    18241 aggcaaagaa atgaggttgc cccgcaagca gggcaacctc gactggcagt atcattttcg
    18301 atcacggctt ccagtttctc gagagccaaa ccccggaggg gtcttcggtg agttctgagt
    18361 gcaggccggt gaaatgcatc caggtattcc ccggttgcag ctcaaagggg ctaccgtcct
    18421 gattgaagaa ctggataggg gtgtcccaac cggatttgta ggttgcttcg tattttaatc
    18481 cgtcgcgaaa gaccacagcg cgcccgttgg cattgagaat atgaatctcg tgcaacgtat
    18541 catcgtggtg cagggtttcg atctcggcgt agattaacag gacgttggag aattgcagct
    18601 gttcacctgt gttccggtcg gtcaacggca ccatttctac atccccattc tccttgatac
    18661 tgtcaatcca acgcatatat tttttggttt caggattgta cttccagccc gcgtagttgt
    18721 ttttgccaaa ctggtgagtg agttcccgcg cctcaacgcc gccggctggc ggcagggtgt
    18781 taaacgccat accgttaagg ttttggcgtg tgttgctggc gttggggcgg gcggcgtaat
    18841 acttggtcat ctccgcggaa tcggcgaata cacttatctc cgctcgcaca ttttcctggc
    18901 ggcacattgc cgggcaggta ttggtgcctt cagagataac cctgctccaa cccaggtaac
    18961 ccaggatggt ttcccaggtg gagggaaagg cgtaaacagc gcccaacaca ccttgataca
    19021 ttcccaccag gtaacgatct atcagcctgc cggaacgaat gggcccgatc tgattggaat
    19081 cttgtccgta aaataacgcc agataacgat ttccgccctc gccagtgtaa taatcaaata
    19141 caatatccgc cgcagacaaa ccggcgtgcg ggcgaccttc acgcgggaag ttggaaacct
    19201 tgatcatcat cggtcgacga tcaaggatag aggggtcagc tacctcaaga ccggtgagag
    19261 ggtctacatt tggtgggaag ttatcaggtc cataacgcac aatggactgt gtaggggtag
    19321 cagtgggctg aggagtggct gtgggcgttg gggtgttggt tgcagtgggc atcgcggcga
    19381 tggtctgctt cacggcttct tccaggctga tttgagtcgc cgttggcgcc gtgccgcctg
    19441 cgcaggcggt gagcaataac atcagcgcca taagaacgca aacccacaga tggttggtct
    19501 gtttcataga atctccgagt tttccaggtt gatcaatacc cagaaattat aacccagctc
    19561 gctaacgttc caacttctaa aggagtttag cggttgacag tgaactgaag attttcaccg
    19621 ccagacgtgg atgaatagat caccccagtt ggagagccaa gtgttccgag caggagtttc
    19681 catccgcctt caggacccga tgcgccaata tagcggttgc catcaatcca gaccaggtcg
    19741 cgagtattat tgaagtcggg tatagataca ggcgcggaac ccggttgacc gatccaggcg
    19801 ccgctggcat catcgcgata gaagaacttg ttattgtcgg gcgaccatac tggaatcctg
    19861 ttaatcttgc cagtggtgta atcggtcaaa ccgctgccat cgatattggc caggcgcagg
    19921 gtatacaggt tatcggttag cgacccataa ggcaccagga aagccagttt ggaaaggtca
    19981 ggagagatgg cggcgatttc cataggtctg aaactcattt gggtgttaaa aagcaattcg
    20041 cttgacagtc catccgccga aatgcggtac acgctcgaat tggcaggcga ggttgcccag
    20101 ggctcctggg gcggaacgac tg
//
LOCUS       DAOSPM010000027        19642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2766_length_19642_cov_12.156890, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000027 DAOSPM010000000
VERSION     DAOSPM010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19642
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2766_length_19642_cov_12.156890"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLW25_03920"
     CDS             <1..426
                     /locus_tag="PLW25_03920"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOS53669.1"
                     /translation="KETERKWVLKIIEEHETGKSVRLAICLKENNQLIGYIHLSDIDY
                     QNRSCEIHTLIGSKEHQGGGFGTEARIMILRYAFLELGMERVRSRIIANNAGSIKSGI
                     KSGYTYEGTLRKAVFQDGQFFDVLLFSILKEEFLQKFNG"
     gene            453..1556
                     /locus_tag="PLW25_03925"
     CDS             453..1556
                     /locus_tag="PLW25_03925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS53670.1"
                     /translation="MRRTPTISIFLNSYNHEKFLRESIDSTLGQTFTDYELIIKDDAS
                     TDSSWDIICSYSDSRITAFRNEVNRQRQFGHFLPDLRGQYVAMHNSDDVWEPTKLEKQ
                     VAYLEAHPEVGAVFTRVVFIDENSRPISGNISFPINFFNQPNRDRFGWLNYFFYYGNA
                     LCHPSVLIRKECYEKCGCYRRGLVQLPDFDMWVRLCLKYEIHVLQEKLVNYRLLLDSA
                     NASSPTLINLIRSNFEKLQIYQNYLALTNRDELVRVLPAIIRYEVGEYYNHRYLIARA
                     ALDKEDDPVCWLFGLQVLFDLLNDPREAAALKQYYQFTPNTFSQLSGSRDVFHEEVHQ
                     QEYQLVHFIKRKIWKWIKPLRDIENLLKKIKKN"
     gene            1846..2817
                     /locus_tag="PLW25_03930"
     CDS             1846..2817
                     /locus_tag="PLW25_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS53671.1"
                     /translation="MRMIIFIPAFNEEGRITETVANIPRMYDGVTDVSVMVVDDGSTD
                     KTAELASVAGAEVVSHTRNRGVGAAFHTAVQYALQHNCDILVGIDADGQFNPLEIPTL
                     IAPLLKGKADMVIGNRFLSGKPANMPGIKFIGNKMVSRFISKISGQVFTDVSCGFRAY
                     GREALLRFNIYGEFTYTHETILSSVYQGLKVVEIPIAVTYYTDRKSRVAGSISKYAAR
                     TSQIILRVLLDYRPLRVFGVIGGGFLAIGMGFGLFLFFHYILTGSFTPYKSFGFIALG
                     FGLFGLIILVIALVADMLNRMKTNQDKILYEIRSLRYQNTTPKDKPQ"
     gene            2814..3893
                     /locus_tag="PLW25_03935"
     CDS             2814..3893
                     /locus_tag="PLW25_03935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS53672.1"
                     /translation="MTQERTLPKVLFIARNYPPIIGGLENNAYNFYQHMSAITETHLL
                     ANPQGKSRLVQFFIETFFYLIFHSSRFDIIHFNDAVMAPFIPVIRLFSRAKITFIING
                     LDIVYHHPLYQLIIPFFLKRADLLFPVSLNTKRECEKRGINPQRLQHIPNGINLKDLP
                     IIESQSAQEFLTKHSINSLHKKILVSVGRLVERKGNAWFLQEVFPLLPEDYIYIIAGN
                     GPQRQRILEIIEQKEWSDRVYLVGWITEDEKLAMFRCADLFIMPNISIKNDLEGFGIV
                     ILEAGYTNLPVVATDVDGIPSAVIPGATGILVPEGDATSFADAILNIHLDREKISLVV
                     DECYNWTKIADQYLQAFVRLTQTTN"
     gene            4145..5815
                     /locus_tag="PLW25_03940"
     CDS             4145..5815
                     /locus_tag="PLW25_03940"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS53673.1"
                     /translation="MANRFIVKQKIRKANISYRWFKKLQDNAPLPIFTGSPVLPAVTF
                     FLAVRKGTENLADRTIDSLQNLASPYWQVVPVLEDSPIPIGQSAERSADPRIKPAVQW
                     NEFALGQFITDQDGASVFCDSGDIFDKSLLNHYFAIVGSGVKKDIIYYDCEYLSSVGG
                     PKPFFKPSALTPELLISNNYFSRGVFRNASMAAVKVKIEEFSNLRALEYALALKLAAR
                     HCSIQHIPATLVSIQERNDAGKDTLQPVLKNHYTGLGFRDVEIQHDGNGRKINWQHGN
                     PSAAIIILSRNHGNWLRTLVQSIQTRTDYANYSIVIVDNQSTETELLNYYQELEDNPQ
                     VKVVPYPQEFNYSTAINLGVSSTSAEIVVLLNNDMQVIEPSWLNELVQWASHPEIGVV
                     GARLLYPNRTIQHAGFIIGMNNFSGHLYLNAPENYFGLTGSVMWYRNFHAVTGACQAM
                     CRTLFEQLGGYDERYRLVFGDVHFCLRAENAGFRNLYNPYATLIHFEGGSRGVETPRD
                     DIQLGCEEIIDNFEGGDPFFASHLSYTNIPYCLLPKKGNPITRKEERA"
     gene            complement(5963..7633)
                     /locus_tag="PLW25_03945"
     CDS             complement(5963..7633)
                     /locus_tag="PLW25_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53674.1"
                     /translation="MLKYLKISFSFIRRYGGIFLLFIPGVVILIHIFGITLSRIPGDL
                     GDSRMVWYMLEHFYAWISGKIDSYWNAGFFYPYPSTAAFSETMLGSAPLYALFRFLGF
                     SKYLSYQFWLILGFMLNYFTAAFILVKCSLKPLAAGAGAFFFAFGMPIFGQEYHLQLI
                     LRFLVPVAGYLLWRFSQKPRFVLLPLLFFAVVWQFYISVYTGFFLAIMLLVMLFLIPF
                     INGGDTSRNPFRFWSSALVLSFKSTSIPKRILHLFLMAASLFLLAKLFMPYYVVTQEY
                     GFKRSIDYIITMMPRLESFFLADGSQIWNTKTWITKFISHRHEQQLFPGLSVYLLIAA
                     GLVWNFRHANKRFALVNLFTVVVILLLTTYNNGFSPYVYLLNLPGFNSIRTVCRIHLV
                     SMWPVAVFVATVLDALLSAPVKKINFRWVSFLLIGFMLMESLYFDHPTYEKTQAAERL
                     SVLYNQIEKKGLIVKEDSVLYVGEVPGDIPYATDVDGMWLGLELGIPTLNGMTSNFPD
                     GAVVASSDCGRIDSFIRSYMKFASIKEAQFRDQLFKRLIILNMDHCNY"
     gene            complement(7754..7993)
                     /locus_tag="PLW25_03950"
     CDS             complement(7754..7993)
                     /locus_tag="PLW25_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOS53675.1"
                     /translation="MNIQERLTKVFRAVFDNESITLTPDLTADDIDEWDSLSHINLML
                     AIELEFGIEFLQSEIQDFRNVGELIECVKTKLEPS"
     gene            complement(7997..9607)
                     /locus_tag="PLW25_03955"
     CDS             complement(7997..9607)
                     /locus_tag="PLW25_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS53676.1"
                     /translation="MKKVENIAYAGLTRFIENGEGEKELLLWVENDLSKTSFSYSYFE
                     TMSNRIAGILQEIGVNPGDTVSLFIPRSPMLISCFFAVLKNAAISSILFSTLGEAGLL
                     DRLSNSNARVIITKKSLLRKIISIKHQLPTLERILVVDIDEDQNDHVLSLKRLLSKSS
                     SAFDYPHSLDAETPAFLQYTSGSTGKPKGALHVHGAIADIEKSFKEIMQLQADDLYWC
                     TADPAWITGLVYGIIAPFTTAARQLQYNGTYNADNWLKLLEQEKVTVWYTAPTALRML
                     MQEEESKFSQRDFQHLKRMYSVGEPLNPEIYHWGKRIFGVEVYDNWWQSETGSMMIAN
                     RPPMKVKPGSMGKPREGIESFICDENMRLLPPGKQGLLCLHKGWGSMFRGYFHNDTAY
                     AEKFHGDIYVAGDLAFTDSDGYFWYVSRADDVINTAGHLVGPFEVESALLEIPEIADV
                     AVIGADDPIMHKRIVAFVKLRSGTAWSKELELKCRVHVSNKVSTSAIPAEFHMIEQVP
                     KNQSGKILRRVLKAVYEGKDPGDTSTME"
     gene            10139..10879
                     /locus_tag="PLW25_03960"
     CDS             10139..10879
                     /locus_tag="PLW25_03960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS53677.1"
                     /translation="MTQKKDRRITQTEDAFIRDDLEQMKFAQNYNRWLYSLVEPFIGQ
                     RVLEIGPGIGNMTGLMLNHVELLVGMEPNPYCVDVLKDLMPKSSNYTILHTKIEDGDT
                     AALKSYRFDTILCMNVLEHIENDVSVLKTFEEILSTGGKVVLLVPAMPVAFGPIDQAV
                     GHFRRYTARTMKKALAETNLKLQTIFYTNFLGFWGWLFNAKVRRSVRQNEGQIQLFDS
                     LVPILSRLEKIIHPPIGLSLITVAIKEE"
     assembly_gap    10891..10990
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10991..11913
                     /locus_tag="PLW25_03965"
     CDS             <10991..11913
                     /locus_tag="PLW25_03965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS53678.1"
                     /translation="LIPCFNEEQTIAKVINDFRRELPHADIYVFDNNSQDATASLAAE
                     NGAIVVKEKRQGKGYVINSMFTDVVSDIYVMVDGDDTYPASSVHELIAPVVNEEADMV
                     VGQRLSSYTEGAFPPLHVLGNKLVCSLVNSVFSTNLKDPMSGYRAFNRKVTMQCPVVA
                     TGFDVETEMTLQLLYRHLKITEVEINYRERPTGSYSKLRTFSDGLKVINKIFSIIPSY
                     KPLTFFGGISLGLAVTSLFVGSFVIREYIRYQYIYSVPKAVLATGLMIFAGILLAVGI
                     ILHVLNFRLLEESNNNQKMFSLLDRALRDK"
     gene            12175..12723
                     /locus_tag="PLW25_03970"
     CDS             12175..12723
                     /locus_tag="PLW25_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOS53679.1"
                     /translation="MTISWYVMRSKPNREEFLARELESRNVEYYYPRLRVNPVNPRAR
                     RFKPYFPGYLFIHLDLEQVNVLSLEHVPGAASLVSFGGEFSHVPDNILHAIRRKVDAM
                     NEKGGQSAADLHAGDQVEIESGPFAGYMAILDATLPGSERVRVLLQMLERRELPVEMP
                     AHFVKVRHSSSGKKRIPRLPES"
     gene            12976..14424
                     /locus_tag="PLW25_03975"
     CDS             12976..14424
                     /locus_tag="PLW25_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOS53680.1"
                     /translation="MRFLKRLVDLLGAAFGLLITSPLFLYIAIRIKRESPGPVYYHGE
                     RVGRYGKPFKILKFRTMYETPQKNSGLRLTVKNDERVTPFGAWLRETKMNELPNFWNV
                     LVGEMSLVGPRPEDPEFVDNWPEEVKTEVLSVRPGMTSPASVIFRDEENMLVGSSVLD
                     DYLISILPEKLRLDQLYVRHFNFFGDIDVILMTIVMLIPGLRQRKVKEGTLFEGPLVR
                     FGRAYISWFMIDSIIAFLAISIATLLWRIQIPLDVGFGRMLLLAVGLAFILGFTNALL
                     GLKKVSWRYASLTHVFDIALSTTLALLVFALLSPPLLSIQLPINLLINIGLLSFTGFV
                     LSRYRERLLTGLASRWIRWRGQSSLIGERVLIVGAGDAAQLAIWLMEKSSLYSAFSIV
                     GLVDDDFRKLKKNINGYPVLGTTREIPEIVKQKSVGLVLLAITRLSDAERERILVTCK
                     ELQVRVVAIPDLLKVVNDYLSKQTQEVMENNA"
     gene            14417..15412
                     /locus_tag="PLW25_03980"
     CDS             14417..15412
                     /locus_tag="PLW25_03980"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOS53681.1"
                     /translation="MPEHWQRKKVLVTGAGGFIGSHLVGALVERGAEVRTFVRYNSRG
                     DPGLLRMLDARKTESIELVAGDLRDEDAVRAAVKGCEVVFHLGALISIPYSYKHPAEV
                     VSTNIMGTLNVLLACRDSGVERLVHTSTSEVYGTARVAPIGEDHPLQGQSPYSASKIG
                     ADKLAESFSCAYDLPLVTVRPFNTYGPGQSGRAVIPTIITQALCRDVVQLGNLEATRD
                     FTFIEDTVAGFIQAAENQNSLGEVFNLGTGEEIAIGELARRIIKIIGSPAAIETKTLR
                     LRPRKSEVMRLVSDNTRARELLGWQPRVSLEDGLLLTIDWVKKNLHQYRVGQYEF"
     gene            15402..16130
                     /locus_tag="PLW25_03985"
     CDS             15402..16130
                     /locus_tag="PLW25_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOS53682.1"
                     /translation="MSSKNAQVHAVVLAGGKGTRLAPYTRIIPKPLMPIGDMPILEIM
                     LRQMKRAGIRQVTLTTGYLDNLLRLFFLDGRRLGLKIDYVREEKPLGTSGPLANVKGL
                     TRTFLVTNGDVLTDLDFAELIAYHQQQGAIATIATHKRKVTINLGVVDVDKDNTVTDY
                     LEKPDFNYLVSMGLYVFEPRVLNYIAKDEYLDFPDLVRSLIAAGEKVSSFTFKGYWED
                     LGRPDDYERASADFERNRSQFLPE"
     gene            16134..17273
                     /locus_tag="PLW25_03990"
     CDS             16134..17273
                     /locus_tag="PLW25_03990"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS53683.1"
                     /translation="MNWIVPLADVKFGVEEQEAVRAVLESGWLTMGEVTAQFEADFKA
                     YTGARHAFAVSNATVGLHMACLALGIGPGDEVIVPSLTFVATANAVRYTGAQPIFADI
                     TSENDLTISLDAIKQAVTPRTKALMLMHYAGYACDMPAILQWAQEQGLMVIEDAAHAV
                     GSFLQEKHLGTWGNAGVFSFFSNKNLSTGEGGMVVTDDDALAAQFKLLRSHGMTSLTW
                     DRHQGHAWSYDVVTTGYNYRIDEIRSALGRVQLEKLDGNNARRSALSARYRELLAELC
                     PALGLPFEGHRGISAHHIMPVLLPRGAQRVRFMERLKEQGIQTSIHYPPVHTFSAYRQ
                     TATGAFNLPITDEVAAREVTLPLYPTMTEDQVQLVTLAAARALEC"
     gene            17294..18547
                     /locus_tag="PLW25_03995"
     CDS             17294..18547
                     /locus_tag="PLW25_03995"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOS53684.1"
                     /translation="MAFSQPTMSLINLLAYPWEGTLAEKTSPLAPEDWRALLEEAEKR
                     ALTLTLYYRLRVAHGEALVPEWVWEHLQTVYLAATARNMVMLHHAAGILRALQERGIE
                     VIVLKGLYLVEQVYPEIGLRTFSDLDLLVRREQLADALTLMQNQGYRLSTWYDPQAQN
                     TDIKHLPPLEKAQHPTLELHWSILEEDEPFEIETAGLWERAVETTIAGVKTRALDSED
                     FLLHLALHFTYQHRLRGGLRNLYDIAEVLEQRMARMNWQKLLATAQQWGAQRVTWLTF
                     QLVEGITRVTAPKEVMTALLPEGADSSAVGAALEQAGSLDDAWAGLTPDLAAFSAAGF
                     FGKIKLLIQRVFISHRVLAREYNINPASPLIMVYYLARVRDLWRRYARSGWRMVAGEE
                     EALAGADFEQQNTRLREWMRGTGKF"
     gene            18626..19396
                     /locus_tag="PLW25_04000"
     CDS             18626..19396
                     /locus_tag="PLW25_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53685.1"
                     /translation="MAAAVVILALIVMLTFVINGPAHMLTSKEIALEQTRISLHILIK
                     QTEVARTSTAELYLTLGVPFPTETPLIVPTSGSLGKPFGTLNPANNADGRVAEGGLGQ
                     PAQTGLPLSDSVLPQSTQPPFTATATGHPAQNRTATATPAPSANPLAGWNGKWTVYFG
                     ADWEELTIGEVNVTVSGTRLNAVGDFSHGRMTISGEISADGQQVTGDYSFNGVSAPFF
                     WQLYGPGQFGGNMAGSRQFCGSKGNLPKPEACGIYFEQ"
     gene            complement(19381..>19642)
                     /locus_tag="PLW25_04005"
     CDS             complement(19381..>19642)
                     /locus_tag="PLW25_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53686.1"
                     /translation="LLTQLRALSVVTAITSPIIPFTRAFTLTGTTKKWVEFFYDYLPM
                     KNYRAKINFVNIFSIGFPCFPPLVENLPFCLRAKPSKGYCSK"
BASE COUNT         5029 a         4642 c         5026 g         4845 t
ORIGIN      
        1 aaagaaacgg aacggaaatg ggtgcttaag ataattgagg agcatgagac cgggaaatcg
       61 gtaaggctgg ccatatgctt aaaagaaaac aatcaactca ttggttacat ccatttatct
      121 gatattgatt atcaaaaccg gtcatgtgag attcatacct taatcggaag caaagagcac
      181 cagggaggtg gcttcggcac cgaagccagg atcatgattc ttcgatacgc ttttcttgag
      241 ttgggcatgg agcgggttag atccaggatc atagcgaata acgccggttc gatcaaatca
      301 ggaataaaat ctggatatac atatgaaggc actttaagaa aggctgtttt tcaggatggg
      361 cagttctttg atgtcttgct cttttctatc ctgaaggagg agtttttaca aaaattcaat
      421 ggctgaatcc ccgggttaag gaaacagaag atatgcggcg aactccaacc atttccatct
      481 ttctcaactc atacaatcac gaaaaatttc tgcgcgaatc tatcgacagt accctcgggc
      541 aaaccttcac tgattatgaa ttgattatta aggacgacgc ttccacagat tcttcgtggg
      601 atatcatctg cagttattct gactctcgga taacggcatt cagaaacgag gtgaaccgtc
      661 agcgccaatt tggtcatttc cttcctgatt tgaggggaca atacgttgct atgcacaact
      721 cagatgacgt atgggaaccc accaagcttg aaaaacaggt cgcctacctg gaggcccacc
      781 ccgaagttgg cgcagtgttc acgcgcgtgg tgtttatcga tgaaaacagc cgcccaatca
      841 gcgggaatat ttctttcccc attaacttct tcaaccagcc caaccgtgac cgttttggct
      901 ggttgaacta ctttttttac tatggtaacg cgctctgcca tcccagcgtt ctcatccgca
      961 aggaatgtta tgaaaaatgc ggctgctacc gccgcggttt ggtgcaattg cctgattttg
     1021 atatgtgggt gcgattgtgc ctgaagtacg agatccacgt gctgcaagaa aaattggtga
     1081 attaccgctt gctgcttgac tccgctaacg ccagcagccc cacgctgatc aacctgattc
     1141 gcagcaactt tgaaaagctg caaatatacc agaattacct ggctctaacg aatcgcgatg
     1201 aactggtgcg ggtgttaccc gccattatca ggtatgaagt gggggagtac tataatcatc
     1261 gctatttgat cgcgcgcgcc gcgctggata aggaagatga tccggtatgc tggctgttcg
     1321 ggttacaggt gttgttcgac ctgctgaacg atccgcggga agcagcggct ttgaagcaat
     1381 actatcaatt taccccgaac acttttagcc aactgtcagg ttcgcgcgat gtgttccacg
     1441 aagaggtgca ccagcaagag tatcagctgg ttcacttcat caagcggaaa atctggaaat
     1501 ggataaagcc gcttcgcgac attgaaaatc tcctcaaaaa aattaaaaaa aactagccgt
     1561 tccatggttt ttcaagaaga accgctggca atataagagg ctgattcccc aggctctgac
     1621 ccttaaatca cttttacacg gcaagcctga ggaaggtcat cttgcctgag cagttgtatt
     1681 ttctggttat gatcatcggt gaataaaaaa gcagtacaac cggttttgta tgcccgtcgt
     1741 aaaatgcgat ggtcattcct gtgacacaaa aggaaaacga aaggatataa tcttgataag
     1801 ctgcacgtta tgttttgcat ataagagccc gtatcggagt ttctaatgag aatgatcatt
     1861 tttatcccgg cctttaacga ggaaggacgt atcacagaaa cggttgccaa cattccaaga
     1921 atgtatgacg gggttacaga tgtgtctgtt atggtagtgg atgatgggtc aacggataaa
     1981 actgccgaac ttgccagcgt ggcaggcgcg gaagttgtat ctcacacgcg caaccgcgga
     2041 gttggcgctg cgtttcatac cgctgtccaa tatgcgctgc agcataactg tgacatcctg
     2101 gtgggcattg acgctgatgg tcagtttaac ccgctcgaaa ttcccaccct gatagctccc
     2161 ctcctcaaag gcaaagccga catggtgatt ggaaaccgtt ttttgtctgg caagcctgcg
     2221 aacatgcctg gaattaaatt cattgggaat aagatggtct ccaggttcat cagcaaaatc
     2281 tcaggtcaag tatttaccga tgtgtcctgc ggttttcgcg cttatggccg cgaagcattg
     2341 ctgcgcttca acatctatgg agagttcaca tacacgcacg aaaccattct gtcatcggtt
     2401 taccaggggc taaaggtggt tgagattcca atcgcagtaa cctattacac cgatagaaaa
     2461 tcaagggtcg ccggatcaat ttctaagtac gctgcgcgaa ccagccagat cattcttcgc
     2521 gtgctgctgg actatcgacc gctgagagtc tttggcgtga taggcggtgg tttcctggcg
     2581 atcggcatgg gtttcggtct gtttctcttt tttcattata tcctcacggg tagtttcact
     2641 ccatataaat cttttgggtt tatcgctttg gggttcggac ttttcgggtt gattatcttg
     2701 gtgattgccc tggtggcaga catgttgaac cgtatgaaga ccaaccagga taaaatcctt
     2761 tatgaaatcc gcagcctgcg ctaccagaac acaactccga aggataaacc gcaatgaccc
     2821 aagaaagaac acttccgaag gtcctgttca tcgccaggaa ttacccgcca atcattgggg
     2881 ggctggagaa caacgcctat aacttctatc aacacatgtc ggcgatcact gaaacacatc
     2941 ttctggcgaa cccgcaggga aaaagccggc ttgtccagtt tttcatcgaa acattttttt
     3001 atttgatctt tcattcaagc cgtttcgaca taatccattt caatgatgcg gtgatggcgc
     3061 cttttatccc ggttattcgc ctcttttcca gggcaaaaat cactttcatc attaacggcc
     3121 tggatatcgt ttatcatcac cccctctacc aattgatcat tccttttttc ttaaaaaggg
     3181 ctgatttgtt attccccgta agccttaata cgaaacgtga atgtgaaaaa agagggatca
     3241 atccgcaacg gttacagcac atcccaaatg gcatcaatct caaagacctg ccaatcatag
     3301 aatcacaaag cgctcaggag tttttaacaa aacattccat taacagcctt cacaaaaaaa
     3361 tcctggtgag tgtagggagg cttgtggaaa ggaaagggaa tgcctggttc ctgcaagagg
     3421 ttttccctct tttaccggaa gattacattt atattattgc cggtaacggc ccgcagcgcc
     3481 agaggatttt ggaaatcatc gaacaaaaag agtggtcaga ccgtgtgtat cttgttggct
     3541 ggatcacaga ggatgaaaaa ttggccatgt ttcgctgcgc tgacttgttt attatgccca
     3601 acatatccat caagaacgat ctggaaggtt tcggcattgt gatcctcgaa gccggttata
     3661 ccaatttacc ggtcgtagcg actgatgtcg atggcattcc aagcgcggtc atcccaggcg
     3721 caaccgggat tttggtgccg gagggtgacg caacgagttt tgcagatgcc attttgaaca
     3781 ttcaccttga ccgggagaaa atcagcttag tggtcgatga atgttataac tggacgaaga
     3841 tcgcagatca gtatctgcag gcatttgttc gcttgacaca gacaacgaac tgagccttgt
     3901 tttattccga aagggttatt tctaaaaacc agaaaaaacg agtaaataac tgtgccagaa
     3961 caaagatatt ttcaattccc gccaaggata gagatacgac ccaaaggcat cctttcttgc
     4021 gctttggggg atttacttga atacgaatat gctgcgagat agaacaagca aaaaaatctt
     4081 cgacatattc ttccctcggg gaagccgcca cgaattgttc ttgcggaccc tttaccataa
     4141 agttatggcg aatcgattca tcgtcaagca gaaaatccgc aaggcaaaca tatcctatag
     4201 gtggttcaag aagctgcagg acaacgcccc cctgccgatt ttcactggct caccggtttt
     4261 gccagcagtc actttttttc tggctgtaag aaaaggaact gaaaacctcg ctgacagaac
     4321 gatcgattcc ttgcaaaacc tggcatcgcc ttactggcag gttgttcccg tgttggagga
     4381 ttctccaata cccattgggc aaagcgcgga gaggtcagca gaccccagga taaaaccagc
     4441 ggttcaatgg aacgagttcg cgctaggcca gttcataact gaccaggatg gcgcgagcgt
     4501 tttttgtgat tcaggcgata ttttcgacaa atcactttta aatcattatt tcgccatcgt
     4561 gggcagtggg gtaaaaaaag atatcattta ttatgactgt gaatatttga gttctgtggg
     4621 cggaccaaaa ccttttttta agccctccgc gctgacccct gaattattaa tctccaataa
     4681 ctatttttct cgcggcgtgt ttcgcaatgc aagtatggct gctgtgaagg taaagataga
     4741 agaattcagc aacttgcgcg cgctggaata cgcgctcgcg ctcaaattgg cagcgcgtca
     4801 ttgttccatc cagcacatcc cagccaccct ggtgtctatt caggaaagga atgacgccgg
     4861 caaagatacg ctgcagccag tactcaaaaa tcactacacg gggcttggct tcagagatgt
     4921 ggaaattcaa catgacggga atggcagaaa aattaactgg cagcatggca acccgtcggc
     4981 tgcgatcatt atattaagca ggaatcacgg taattggctt agaacgctcg tccaatcgat
     5041 ccaaactcgt acagattatg cgaattattc gatcgtgatt gtggataatc agagcactga
     5101 gaccgagcta cttaattact atcaggagct ggaggataac ccgcaggtta aggtggtgcc
     5161 ttatccccag gagtttaatt attcaactgc cattaatctc ggcgtatcga gcaccagcgc
     5221 tgaaattgtt gttcttctca ataatgacat gcaggtcatt gaaccttcat ggttgaacga
     5281 acttgtgcaa tgggcttcgc atcctgaaat tggggtggtt ggtgccaggc ttctctatcc
     5341 caaccgcacg atacagcacg caggttttat tatcggcatg aacaattttt ctggtcattt
     5401 gtatttgaac gcacctgaga actactttgg gttgaccggt tcggtcatgt ggtaccgcaa
     5461 ttttcacgcg gtaacaggtg cttgccaggc gatgtgccga actttgtttg agcaactcgg
     5521 aggctatgat gagcgttacc gcctggtgtt tggagatgtg catttctgtt tacgggcaga
     5581 aaatgccggc tttcgcaacc tgtacaatcc ttacgccaca ctgatccact ttgaaggcgg
     5641 ttcccgagga gttgaaacgc cccgggatga tattcagtta ggatgcgagg aaatcatcga
     5701 taattttgag ggcggcgatc ccttttttgc cagtcactta tcctacacaa acattcccta
     5761 ttgccttctg ccaaagaaag gaaatcctat cactcgtaaa gaggagcggg cgtagtgttt
     5821 tcacgctggc ttttgctgtt tatcaatcta accgatagga agttctaacc caattaagga
     5881 aataatgagt ttgcaggacg acacctgcgt ggttctttga attacgcaac tgatctaaaa
     5941 ctcgatcgtc cgttcccgca gctcaatagt tacagtggtc catgttcaag ataatcagcc
     6001 gcttgaataa ctggtcgcga aactgagctt ccttaatact ggcaaatttc atataactcc
     6061 ggatgaagga atcgatccgg ccgcaatcag aagaggcgac aacggcacca tccggaaaat
     6121 tactggtcat gccgttgagc gttgggattc ctaattccag gccaagccac atgccgtcaa
     6181 catcggtggc gtagggtata tctcctggaa cttcaccgac atacaggacg gaatcttctt
     6241 taactatcaa ccctttcttt tcaatctgat tgtacaggac gcttaaccgc tcagcagcct
     6301 gggttttttc ataagtgggg tgatcaaaat agagagattc catcaacata aacccgatca
     6361 gcaaaaatga aacccatcga aaattgattt tttttaccgg cgcgctcaat aatgcgtcga
     6421 gcacagtggc aacgaaaaca gccactggcc acattgaaac gaggtggata cgacaaacag
     6481 tgcggatact attgaacccg ggtaaattca gtaaatacac atatggtgaa aatccattgt
     6541 tgtaagttgt caacaagagg atgaccacca ctgtgaaaag attgactaaa gcgaaccgtt
     6601 tattggcatg ccggaaattc caaacaagcc ctgccgcgat caagaggtaa acggaaaggc
     6661 ctggaaacaa ttgctgttca tgacgatgag aaatgaactt ggtgatccag gttttggtgt
     6721 tccaaatctg cgagccatca gcgaggaaga aactttcgag cctgggcatc atggtgatga
     6781 tgtaatctat cgacctttta aatccatatt cctgagtaac tacgtaataa ggcatgaata
     6841 acttcgccaa aaggaacaga ctggcggcca ttaagaaaag gtgaagaata cgcttcggga
     6901 tgctggtgct cttgaaagac aatacaagcg cggaactcca aaaccgaaat ggattccggg
     6961 atgtatctcc tccattaata aaagggatga ggaataacat cacgagcagc atgatggcaa
     7021 gaaaaaaccc agtatagacc gagatataaa actgccagac aaccgcgaag aacaacaggg
     7081 gcagcaacac aaagcgaggt ttctgtgaaa aacgccacag caggtaaccc gccaccggga
     7141 caagaaaacg cagtatcagt tgcaggtggt attcctgacc aaaaatgggc attccaaagg
     7201 cgaagaaaaa cgcgcctgcg ccggcagcca gtggtttaag agagcacttc accaggataa
     7261 aagctgcggt gaagtagttc agcataaacc ccagtatcaa ccagaactgg taggaaaggt
     7321 atttgctgaa cccaaggaat cgaaaaagag cgtacagcgg agctgaacct aacatcgtct
     7381 cgctgaacgc ggcagtcgat gggtaaggat aaaagaaacc cgcgttccaa taggaatcaa
     7441 tttttccgct gatccaggcg tagaaatgct caagcatata ccagaccata cgggaatcac
     7501 ccagatcgcc tggtattctg gataaggtga taccaaaaat atggataagg atcacaaccc
     7561 ctggaatgaa caacaaaaaa atgccaccat agcggcgaat gaaagaaaag gagattttta
     7621 gatacttcag catgtgtttc ctaccttggc accgaaagga cattgcttaa aaatagcgaa
     7681 ttatcgtcca ttttcaactg ggggcaaaac cccagtgaag gtgatcttct ctctcgctgc
     7741 ggcttgttaa ggtttaagat ggctcgagtt tcgtttttac acactcaatc agttccccaa
     7801 cattgcgaaa atcttggatc tcgctctgta aaaattcaat gccaaattcg agctcgatag
     7861 cgagcatcag gttgatgtgt gagagagaat cccattcgtc aatatcatcg gcggttaaat
     7921 caggggtcag tgtgattgat tcattgtcga acacggcgcg gaaaaccttc gttaatcttt
     7981 cctgaatatt catctttcac tccatggttg aggtatcgcc agggtctttc ccttcataaa
     8041 cagccttcaa cacccggcgt agaattttcc cgctttggtt tttgggcact tgttcaatca
     8101 tgtggaattc agcggggatg gcggaagtgg atactttatt tgacacatga acgcggcatt
     8161 tgagttccag ctctttgctc caggccgtgc cgctgcgcaa tttcacaaac gcaacaatcc
     8221 gtttatgcat aatggggtca tccgccccaa ttacagccac atccgcgatt tctggaattt
     8281 ccaacagcgc gctttcaact tcaaatgggc caaccaggtg cccggctgtg ttaatcacat
     8341 catccgcgcg gctcacatac cagaaatacc catcgctatc tgtaaacgcc aggtctccgg
     8401 caacgtaaat atcgccgtgg aatttttcag cataagccgt gtcattgtgg aaataaccgc
     8461 gaaacatcga cccccatcct ttatggaggc ataacaggcc ctgcttgcca ggcggcagca
     8521 accgcatatt ctcatcgcaa atgaacgatt caatgccttc tcttggcttc cccattgacc
     8581 cgggcttgac cttcattggt ggtcggttgg caatcatcat tgacccggtc tccgattgcc
     8641 accagttatc ataaacctcc acgccgaaaa ttcgcttgcc ccaatggtag atttcaggat
     8701 ttagtggttc accaacgcta tacatgcgtt tgaggtgttg aaaatctcgc tgagagaatt
     8761 tactctcttc ctcttgcatt aacatacgca gcgctgtagg ggcggtatac caaacggtca
     8821 ccttctcttg ctccaggagc ttcagccagt tatcggcgtt ataagtgcca ttatattgaa
     8881 gctgacgcgc cgcggtagtg aatggcgcaa tgatgccata taccagcccg gtgatccatg
     8941 ctggatctgc tgtacaccag tacaggtcat ccgcctggag ctgcatgatt tctttgaacg
     9001 atttttcgat atcagcaatc gccccgtgca catgcagcgc cccttttggt tttccggttg
     9061 atccggaggt gtattgtaag aacgccgggg tttctgcatc cagggaatgg gggtaatcga
     9121 aagctgagga acttttcgaa agcaaacgtt tcaagctgag cacatggtcg ttttgatctt
     9181 cgtcgatatc gaccaccagt attcgttcaa gtgttgggag ctggtgttta attgagatga
     9241 tcttcctgag aaggctcttt tttgtgatga tgacccgggc gttactgttt gaaagcctgt
     9301 ccagtaaccc cgcttctcca agcgtgctaa agaggatact tgaaatagcc gcatttttta
     9361 gcacagcaaa aaagcaactg atcagcatcg ggctgcgcgg gataaacaag ctgacagtgt
     9421 caccggggtt gacgccaatt tcctgaagaa tcccggcgat gcggttagac atggtttcga
     9481 aatagctgta agaaaaagac gttttggaga ggtcattttc aacccacagg aggagctctt
     9541 tttctccttc tccgttttcg ataaacctgg ttagaccagc gtaagcgata ttctcgacct
     9601 ttttcatcga acctcaatat caggagatta tcgtacagtt ggaatggttg agattattca
     9661 aagccgctcc cagaccaata tatcatcaaa aacgcgagat gggtagaatt gtcatactga
     9721 aatttgtctt tatgagagtt cagtcacatc catagagctg ttgagggaac aaaaaagacg
     9781 gattctaact tcaaccgatg aggttgtaat tttccgcctg tgacgagtaa aggttaacga
     9841 taagggtaga aacaagaaat tctaagaagc cgaattttta tgcttcaatg gtcgacggaa
     9901 aaaagctgat gagaaagaga ttttcattac gggtataatc gctctggttg agcgtaaaag
     9961 gatatgttgt tagcgccagg taatggggtt gattcatatc gcccaaacgg atggtttctg
    10021 tttaatacct caggaacgta acttttccgt ttagttctcg cactcagctg gatgagtgaa
    10081 gcgggcgcag attctttgac cataaacaat cgatcatgca ggaagaattg attaacacat
    10141 gacacaaaaa aaagaccgca gaattactca aacagaagat gcgtttatca gagatgattt
    10201 agaacaaatg aaatttgccc aaaactataa tcgctggctg tattcacttg tagaaccatt
    10261 tattggacag cgagttttag agattggccc agggattggt aatatgactg gtttaatgct
    10321 caatcatgtg gaattgctgg tgggcatgga acccaatcca tactgcgtcg atgtattaaa
    10381 agatttgatg ccgaaatcat cgaattacac tatcctgcac acgaagatag aagatggtga
    10441 cactgcggct cttaaaagtt atcgttttga cacgatattg tgtatgaatg tgctggaaca
    10501 catagaaaac gatgtctctg ttcttaaaac ctttgaagaa atcctttcta cagggggaaa
    10561 ggtggttcta ctggtgccgg caatgcctgt ggcatttgga ccaatcgatc aggcggttgg
    10621 acacttccgc agatacactg cgcgcaccat gaaaaaagcc ttagcggaga cgaacctaaa
    10681 actccaaacc attttttaca cgaacttttt gggtttttgg ggctggcttt ttaatgccaa
    10741 ggtaaggcgt tctgtcaggc aaaatgaagg gcaaattcaa ctttttgatt ctctggtgcc
    10801 catcctttcc agacttgaaa aaataatcca cccgccgatc gggttgtctc tcattacggt
    10861 cgccatcaag gaagagtgac tcttcaggca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn ttctaatccc ctgttttaac gaagagcaaa cgattgccaa ggtgatcaat
    11041 gattttcgta gagaattgcc gcatgcggat atttatgtgt ttgacaacaa cagccaggac
    11101 gcaacagcga gcctggcggc tgaaaacggc gcgattgtgg taaaagaaaa acgccaggga
    11161 aaagggtatg tgatcaactc gatgttcact gatgtcgttt cggatatcta tgtgatggta
    11221 gacggcgatg atacttaccc cgccagcagc gtacacgagc tgattgcccc ggttgtaaac
    11281 gaagaagcgg acatggtcgt cgggcaaagg ctgtccagtt atacggaggg ggcttttcct
    11341 cctctccatg tcctgggtaa caagttggtt tgttctttgg tgaattctgt tttttcgacc
    11401 aacctcaaag atcccatgtc cgggtatcgc gcgtttaaca ggaaggtgac gatgcaatgc
    11461 ccggtagtcg ccacaggctt tgacgttgaa acagaaatga ccctgcaatt actttatcgc
    11521 catctgaaga tcaccgaagt tgaaatcaac taccgggaaa gaccaacggg aagttactcg
    11581 aagttgcgaa cattttcaga tggtcttaaa gtgatcaata aaatcttttc gattattccc
    11641 tcttataaac cgctcacgtt ttttggcggg atcagcctgg ggctggcggt caccagttta
    11701 tttgtgggct cttttgtgat tcgtgagtat atccggtatc aatatattta cagtgtaccc
    11761 aaagcggtgt tagctaccgg gctaatgata tttgctggaa ttttattggc ggtagggatt
    11821 atcttgcatg ttctaaactt ccgcctgctg gaagagtcca acaataacca gaaaatgttc
    11881 tcactgttag accgggcgtt acgtgataaa taggcattcg ctggtttcaa tatttctgtt
    11941 ctttcaagag gtttccgcct ttcagctggc tggaaagccg tgcttggggc tatgggttgg
    12001 gaagagttga ttttgcagcg ggcatgcggg gttggttgaa tgagacggcg gcatcgcgac
    12061 tgaatggcta tggcctgaca ggggtgggaa agaataagtg aaatataggg gttacgagga
    12121 aattggcgtg ttaaagggga aaccgcaggc gattgaaggg gatgaggaat atcgatgaca
    12181 atttcctggt atgtgatgcg cagcaaaccc aaccgcgaag agtttctagc gcgggagctt
    12241 gaatctcgga atgtggagta ttactaccca cgactgcggg tcaacccggt gaacccacgc
    12301 gcgcgccgct tcaagcctta cttccctggc taccttttta ttcaccttga ccttgagcag
    12361 gtcaacgttc tttctctgga acacgttccc ggagcagcca gcctggtttc atttggcggc
    12421 gagttttcac atgtaccgga taatatactt catgccatcc gtcgaaaagt ggacgcgatg
    12481 aatgaaaagg gggggcagtc cgcggcggac ctgcatgcgg gcgatcaggt cgaaatcgaa
    12541 agcggaccgt tcgccggtta tatggccatt ctggacgcta cgttgccggg aagcgaacgg
    12601 gtacgcgtct tgttgcaaat gcttgagcgc cgagagctgc cagtggaaat gccggcgcat
    12661 tttgtaaagg tacgccattc ttcttctgga aaaaaaagga tcccaagact ccctgagagt
    12721 tagcctgcat ggaagcatcg aggtgaatca acgaagtttt gtaaatatat ggtcattttg
    12781 aatctggagg ttcaatcaag ttttaggata tactattaaa tggattcgtt gatcagattt
    12841 cattttctat cgattatgcc acgcgtcgct ggatagagtt cagagccaga attgagccga
    12901 actgttgatt tcttagacaa gcaggcagcc aggggatttt cagcctgggt tttaccgcgt
    12961 cagcggagcg ttttgttgcg ttttcttaaa cgcctggtcg atttgctggg cgcagcgttt
    13021 ggcctgctga ttacctcgcc attgtttttg tacatcgcta ttcgtataaa acgtgaatca
    13081 cctggaccgg tgtattacca cggagagcgg gttgggcgct atggcaaacc cttcaaaatc
    13141 ctcaagttcc gcaccatgta cgaaacccct caaaaaaaca gcggtttgcg gttgacggtg
    13201 aaaaatgatg aacgcgtaac gccgtttggc gcctggctgc gcgagacaaa gatgaatgaa
    13261 ttacccaatt tttggaacgt gctggtgggg gaaatgagcc tggtggggcc gcgccctgaa
    13321 gaccctgaat ttgtggataa ctggcccgaa gaagttaaaa ccgaagtact ttccgtgcgc
    13381 cccgggatga ccagccccgc gtcggtcatc ttccgtgacg aggaaaatat gctggtaggc
    13441 agcagcgtgt tggatgatta cctcatatcc atcttgccgg aaaaattgcg cctggaccaa
    13501 ctctatgtgc ggcacttcaa tttctttggc gatatagacg taatcttgat gaccatcgta
    13561 atgctcatcc cggggctgcg gcagcgtaaa gtaaaggaag gtaccttatt tgaaggaccg
    13621 ttggttaggt ttggccgcgc ttacatctcc tggttcatga tagattcgat catcgctttc
    13681 ctggcaatct ctattgccac gctcttatgg cgcattcaga tccccctgga tgtgggattt
    13741 ggacgcatgt tgctgctggc tgttggattg gctttcatcc ttggtttcac gaacgcgctg
    13801 ttaggcctta aaaaggtcag ttggcgctat gccagcctga cacatgtgtt cgatattgcg
    13861 ctgtcaacca cgctcgcgct gctggttttt gcgctgctca gcccaccgtt gctgtctatc
    13921 caactaccga tcaacctgct catcaatatc ggattgttat cctttaccgg gttcgtgcta
    13981 tcgcgctatc gtgaacgcct gctcactggc ctggcttcgc gctggattcg ctggcgcgga
    14041 caatccagcc tgattggcga gcgtgtgctc atcgtgggcg cgggagatgc cgcgcagctg
    14101 gccatctggt tgatggaaaa gagcagcctg tattcagcgt tctcgattgt gggattggtg
    14161 gatgatgatt tcaggaaact aaaaaagaac atcaacgggt acccggttct aggcaccaca
    14221 cgcgaaatcc cagaaatcgt taaacagaag agtgtcggtt tggtcctttt ggccattacc
    14281 aggttaagcg acgcagaacg tgaacgcatt ctggtaacct gtaaagagct gcaggtgcgc
    14341 gttgtggcga tccctgacct gttaaaagta gtgaacgatt acctgagcaa gcagacccag
    14401 gaggtaatgg agaacaatgc ctgagcactg gcaaagaaag aaggtattgg tcaccggggc
    14461 gggcgggttt attggcagcc acctggtggg cgcgctggta gaaagagggg cagaggtgcg
    14521 cacctttgtc cgttataact cgcgtggaga tcccggcctg ctgcgcatgt tagatgcccg
    14581 caaaaccgaa tcaattgagc ttgttgccgg agacctgcgg gacgaggacg cggtgcgcgc
    14641 agctgtaaaa ggctgcgaag tggtttttca tctgggcgcg ttgatttcca tcccctactc
    14701 atataaacac ccggctgaag tggtatcgac caatatcatg gggacgctca acgttttact
    14761 ggcctgccgc gattcaggcg tggaacggct ggtgcacact tccaccagcg aggtgtacgg
    14821 cacagcgagg gtggcgccca ttggcgaaga ccaccccctg cagggtcagt cgccctattc
    14881 tgccagtaaa ataggcgcgg ataaactggc tgaaagcttc tcttgcgcgt atgatttgcc
    14941 gctggttacc gttcgcccat tcaacaccta tggacccgga caatccggac gggcggtaat
    15001 ccccaccatc atcacgcagg cgctttgccg cgatgttgtt caactgggca acctggaagc
    15061 cacgcgtgat ttcaccttta ttgaggatac cgtggccggg ttcatacaag cagcggagaa
    15121 tcaaaacagc ctgggagagg tgttcaacct gggcactggc gaggaaattg ccatcggtga
    15181 actggcgcgc aggatcatca agatcatcgg cagcccggct gccattgaaa ccaaaacgtt
    15241 gcgtttgcgc cctcgaaagt cagaggtaat gcgcctggtt tcggataaca cgcgcgcacg
    15301 ggaactcctg ggatggcagc cgagggtgtc ccttgaggat gggctgctgc taaccattga
    15361 ttgggtgaag aaaaaccttc accagtaccg ggtaggtcaa tatgagttct aaaaacgctc
    15421 aggtacacgc ggtagtgctg gcaggcggta agggaacccg cctggcgcct tacacgcgca
    15481 tcattcccaa gccgctcatg cccatcggtg atatgcccat tcttgagatc atgctgcggc
    15541 agatgaagcg cgctggcatt cgccaggtga cgcttaccac cggttacctc gataatctgc
    15601 tgcggctctt cttcctcgac ggcaggcgat tggggttgaa gatcgattat gtgcgggaag
    15661 agaaacccct gggcacctcc ggccctctcg ccaacgtcaa aggcctcacc cgcaccttcc
    15721 tggtaaccaa tggcgatgtg ctcacggacc ttgattttgc agaacttatc gcttaccacc
    15781 agcagcaggg cgccatcgcc actatcgcca ctcacaagcg aaaggtcact atcaacctgg
    15841 gcgtggttga tgtggataaa gacaacacgg taaccgatta cctcgagaaa ccagatttca
    15901 attacctggt gagcatgggt ttgtatgtct ttgaaccacg ggtgctcaac tatatcgcca
    15961 aagacgagta cctggacttc cctgatctgg tgcgcagcct tatcgccgcg ggagagaagg
    16021 ttagttcttt caccttcaaa ggatactggg aagacctggg acgaccggat gattacgagc
    16081 gcgccagcgc ggattttgaa agaaaccgca gccagttttt gccggagtaa aacatgaact
    16141 ggatagtgcc tttggcggat gtgaagttcg gcgttgaaga acaggaagcg gtgcgcgccg
    16201 tgctcgaaag cggctggctt accatggggg aggtgactgc gcaatttgag gctgacttta
    16261 aagcctacac gggagcccgg cacgctttcg cggtttccaa cgccaccgtg gggctgcaca
    16321 tggcctgcct ggcgctggga atcggccctg gagatgaggt cattgttcca tcattaactt
    16381 ttgtggcaac cgccaacgcg gtgcgctata ccggcgcgca accaatcttc gcggatatca
    16441 ccagtgaaaa tgacttgacc atttcgcttg atgcgattaa acaagcggta accccgcgca
    16501 ccaaggcgct gatgctcatg cattacgctg gttacgcctg cgacatgccg gcgattcttc
    16561 agtgggcgca agagcagggg ttgatggtga tcgaagacgc tgcccacgcg gtgggctcct
    16621 tcttgcagga aaaacatctg ggtacctggg gaaacgcggg cgtcttcagt ttcttttcca
    16681 acaagaatct ttcgactggt gagggcggca tggtggtgac cgacgatgac gcgctggcag
    16741 cgcagttcaa gctgctacgc tcgcacggta tgacctcgct cacctgggat cgccaccagg
    16801 ggcacgcctg gagctacgac gtggtgacca ccggttacaa ctatcgtatt gatgaaatcc
    16861 gttcggcgct cggaagggtg cagctggaga aactggatgg caacaacgcc cgccgcagcg
    16921 cgttgagcgc gcgataccgc gaactgctgg cggaactgtg tcccgcgctg gggttgcctt
    16981 ttgaaggaca ccggggaatt tcagcgcatc atatcatgcc ggtgctgctg ccgcgtggcg
    17041 cccagcgggt acgttttatg gagcgcttga aagagcaggg cattcaaacc agcatccatt
    17101 acccgccggt gcacaccttc agcgcgtatc ggcaaacggc aaccggcgcg ttcaacctgc
    17161 caatcaccga cgaggtggcg gcgcgcgaag tgactttgcc gctctatccc acaatgaccg
    17221 aggaccaggt tcagttggtg acgctggctg cggccagggc gctggagtgt tgaacgcgcc
    17281 tggcagtatg gagatggctt tctctcaacc aacgatgtcc ttaatcaacc tgctcgctta
    17341 cccctgggaa ggcacgctgg cggaaaaaac atccccgctc gcgcctgaag attggcgcgc
    17401 cctcctggag gaagccgaaa aacgcgcact gaccttaacg ctctactatc gcctgcgcgt
    17461 tgcgcatggc gaggcgcttg ttcccgaatg ggtgtgggaa catctacaga ccgtttacct
    17521 ggcggccacc gcccgcaata tggtgatgct gcaccacgcc gcggggatcc ttagagcgct
    17581 tcaagagcgc ggaatcgaag tgatcgtgct caaagggctg tacctggttg aacaggtgta
    17641 ccccgagatc gggctgcgca ccttcagtga tctggatttg ttggtgcgtc gggaacaact
    17701 ggcggacgcg ttaaccctga tgcagaacca ggggtacagg ctctctacct ggtatgaccc
    17761 gcaggcgcaa aacactgaca tcaagcattt gccgccgctg gaaaaggcgc agcatcccac
    17821 cctcgaactg cactggtcca tattggaaga ggatgaacct tttgagatcg aaacggcagg
    17881 gttatgggag cgcgcggtgg agacgacgat cgccggcgta aaaacgcggg cgctcgactc
    17941 agaagatttt cttttgcatc tggcgttgca ttttacctac cagcacaggc tgcgcggagg
    18001 gctgcgcaac ctttatgata tcgccgaagt gctggagcag cgcatggcga ggatgaactg
    18061 gcagaaactg ctggctacag cgcagcaatg gggcgcgcag cgggtgacct ggctcacatt
    18121 tcagcttgtt gagggaatca ccagggtgac tgccccgaaa gaagtgatga ccgcgctgct
    18181 ccccgaaggg gcggattctt ctgctgtggg cgctgccctt gaacaagcag gcagtttaga
    18241 tgacgcatgg gcgggcctga cgccggacct ggctgcgttc tccgcggcgg gtttttttgg
    18301 caagattaaa ttgctgatcc agcgggtgtt catctcccac agggtgttgg cgcgcgagta
    18361 taacatcaac ccggcttcac cgctgatcat ggtttattac ctggcgcgcg tgcgcgattt
    18421 gtggcgcaga tacgcgcgct ccggctggcg tatggttgca ggcgaggagg aagcccttgc
    18481 cggggcagac tttgagcagc aaaacacccg tctgagggaa tggatgaggg ggacaggtaa
    18541 gttttaagat gaccagtttg gaattttatg atacattaaa cccacgatga accaaaaatc
    18601 cacactgaag aagattggag ttgttttggc ggcggctgtt gtaatcctgg cgctcatcgt
    18661 gatgttaact tttgtcatta acggcccggc gcacatgctc accagcaaag aaatcgccct
    18721 tgagcaaacg cggatctccc tccatatctt gatcaagcag actgaagttg ccagaaccag
    18781 caccgctgag ttgtacctca ctttgggcgt tcctttcccc acagaaaccc ctctcattgt
    18841 gcccaccagt ggttctttag gaaaaccgtt tggcacttta aacccagcca ataatgcgga
    18901 tgggagagtt gctgaaggag gattgggaca gcctgcgcaa accggcttgc cattgagcga
    18961 ttcagttctt ccgcaatcca cgcaaccgcc tttcaccgcc acggcgactg ggcaccctgc
    19021 gcagaatcga acagccactg caacccctgc gccgagcgcc aacccgctcg cagggtggaa
    19081 cggcaaatgg acggtgtatt ttggcgctga ctgggaagaa ctgaccatcg gcgaagtgaa
    19141 tgtgaccgtc tcaggcacga ggttgaatgc agttggtgac ttcagtcatg ggcgcatgac
    19201 catcagcgga gagatctcag cagacgggca gcaggtgacc ggggattatt cttttaacgg
    19261 cgttagcgct cctttcttct ggcagttgta tggccccggg caatttggcg ggaatatggc
    19321 ggggtcacgg caattttgcg gctcaaaagg caacctgccg aaaccggaag cgtgcggtat
    19381 ctatttcgaa caatagcctt ttgagggttt cgctctaaga cagaaaggca agttttctac
    19441 cagcggggga aaacagggga aacctataga aaaaatgttg acaaaattta tttttgctct
    19501 ataatttttc ataggcaaat aatcataaaa aaattcaacc cacttttttg ttgtacctgt
    19561 tagagtaaat gctctagtaa aggggataat ggggcttgtt attgctgtaa cgacagatag
    19621 tgctctcaac tgtgtaagca gg
//
LOCUS       DAOSPM010000028        19582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2780_length_19582_cov_11.614483, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000028 DAOSPM010000000
VERSION     DAOSPM010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19582
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2780_length_19582_cov_11.614483"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLW25_04010"
     CDS             complement(<1..329)
                     /locus_tag="PLW25_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53687.1"
                     /translation="MTPRPSPKDKFPPKKNPAPKEFVPLVERIDAILTHFRQFGWDLL
                     GTFLILLAILTSLGLLGLSRGTLINSWITLIRRAFGLGSYLLVLVLVFVGVSVFRHAA
                     GKRFPLS"
     gene            complement(326..1273)
                     /locus_tag="PLW25_04015"
     CDS             complement(326..1273)
                     /locus_tag="PLW25_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOS53688.1"
                     /translation="MVEIVQERKQEASIPEMDEGWWASVLADEEKFFRENKEVNCKPC
                     ESKTTSQLDWDCIKAVYADDEVVLLEVQGYNRGGLLVQGAGIQGFVPVSHLVDFQAEV
                     SDEVRQQKLANYVGTSLYLKIIECDMESERVVLSERAAQAGEGKRKELFTQLTEGTIV
                     CGVVTNITDFGVFVDLGGVEGLIHVSELSWGRVEKPSHVFKVGEQVKVIVLQVNEANS
                     RIALSHKRLTPNPWERLSKIYKPGDVVSAELTSIMRFGIFARLDEGVEGLIHISAFPE
                     TLRKMNIAEHYTPGQRVQVKILHVDADRRRLGLGLISEE"
     gene            complement(1317..3221)
                     /locus_tag="PLW25_04020"
     CDS             complement(1317..3221)
                     /locus_tag="PLW25_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53689.1"
                     /translation="MMVSNWVNEIIAIARTISEILTAGIAITAFALLLYSLTFNLLER
                     VARTFATIMVCLVIVFSTESFGATSKDPYMIDFWLRAQWVGIILLPAAYLNFSDALLA
                     TTGRPSRWRRRWAIRATYLFSFILIFFLFTPWFLGELVLDQPPAPHHLPTIYTQLFIV
                     YYIIILVLAWVNFRRAYQRTLTRTSKRRMLYLAISAIGPVVGAFPFLLYSSEFASQWT
                     LLFWLLAATVSIGLDICIFIMAYAVAFFGVPWPDRVVKSRLIKWTLRGPITAVLTLGL
                     MTLVKRANIALGTPYDAFVPIVMVVTVLICEYAITLFFPAFERWLIYDRDEQDLRFVL
                     TLEQRIITRGDLKQFIEMVLAAVNDRAQAQGAYLLEKNDEGFTLVEKTPDFSFDPQLL
                     EELDYLESAGNIHEEYVECRSDILIPLHNNSNGSGEHLLGYIGITGIKDISALAAEDQ
                     AALKRLAARAAIALRDRHMLEQVFDTLERLEPQEELIQKLRAVSRFDQRGMLMEADWM
                     EKKEMTTWVKGALTHYWGGPKLTDSPLLKLEIVKEAMAKHDNNEVNALRTVLRSALEQ
                     LKPEGERKYTGEWLLYNILEMKFLEGKKVREIAVKLAVSEADLYRKQKVAIEKIADRL
                     VQMELNTRNT"
     gene            complement(3226..3765)
                     /locus_tag="PLW25_04025"
     CDS             complement(3226..3765)
                     /locus_tag="PLW25_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS53690.1"
                     /translation="MAEVEIRPAVSSDISTLEKFDHTIETTHVWRVDGSTTKDRIAIE
                     LNEVKLPRVLRLNYPRRAELLRNTWTRHLLFLIASCEGSLAGYLTLDEDNDKLLAVVH
                     DLVVNTPFRRQGIASALVFSARDWVKKRGMTRMLLEIPAKNHAMVEMAVKLRFSHAGQ
                     VDNFYQNGDMVLFYLSELK"
     gene            complement(3768..4454)
                     /locus_tag="PLW25_04030"
     CDS             complement(3768..4454)
                     /locus_tag="PLW25_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53691.1"
                     /translation="MSSILRFLEKYEILIFLVAGLIILASARKTYVAWREWSRSIFGL
                     EKEHAQQIFNSALTVLVFSVLLATGLFLVNTFITPAVPGVQQLPTPTIDLTALPPTET
                     PAPILMVTGEGLIPTITSYFSRGCIPDQIDWTDPKNGDTLSGRVELKGTVNIENLGFY
                     KYEYSPSDSDTWTTIAAGNAKIINEPFGGTWDTRALIPGEYKLRLVVNDIQNNSLPAC
                     VILVTIKASE"
     gene            complement(4493..5338)
                     /locus_tag="PLW25_04035"
     CDS             complement(4493..5338)
                     /locus_tag="PLW25_04035"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOS53692.1"
                     /translation="MDSFITIREIDSAANFITRQIVEKPVVALVLGSGLGGLAEAVND
                     ARIIPFNQIPEWPVSTIQGHSGRLVFGRLFGVPAMVMQGRVHYYEGYSMDRVTFPIRV
                     MQRMGVRNLVLTNAAGAINPDYVPGDVMLLTDHLNLIGMAGQNPLRGPNLNEFGERFP
                     DMSQPYDSLFIAAARQVAQKHHIKLHEGVYVCLAGPSFESPADLRFLKIIGADAVGMS
                     TVPENIVARHAKMRVLGLSGISNKANLDGSTVTTHEEVLEAGKVIVPKLTTIIRGVIE
                     TLAGE"
     gene            complement(5417..6460)
                     /gene="ltaE"
                     /locus_tag="PLW25_04040"
     CDS             complement(5417..6460)
                     /gene="ltaE"
                     /locus_tag="PLW25_04040"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOS53693.1"
                     /translation="MKLIDLRSDTVTTPTEEMRQAMAATEVGDDVYGEDPTVNRLQEM
                     SAALLGKEAGLFVPSGTMGNLLSVLSVCNRGDEVIMGNLGHTFLYEAGGISALGGIFA
                     HTLPNQADGSLMLEDIENAIRPDNIHDPITRMVILENTHNRCGGTVLTERYTREVAEL
                     AHRNHLHLHLDGARIFNAAAALNVDVKALTEPVDSVTFCLSKGLCAPVGSVVCGTKEF
                     IKKGLRVRKMLGGGMRQAGIIAAAGIFALENMITRLREDHQRATLLAEGLMTVPGIHF
                     EIGMPQSNMVFLSLKEEVIFSAQEVAERLARLGVKVGVVAQRRFRLVTHYWITDKDVD
                     ETIRAFRSALSGA"
     gene            complement(6474..7340)
                     /locus_tag="PLW25_04045"
     CDS             complement(6474..7340)
                     /locus_tag="PLW25_04045"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOS53694.1"
                     /translation="MDKFLARQLPGQSRSRLQAIIKDGGVTVNETVATKSGLALLTGD
                     KVSICIPEVVPVNLEPESIPLDIVFENEHLVIINKPAGMVVHPAPGHASGTLVHAALG
                     NFGDLQGIGGEVRPGIVHRLDKDTSGLIIVAKNGKAHRWLQEQFRLRKVKKTYLALVD
                     GKPPTPSGRVEAAIARDAAHRKQMTVVSDGRGRAAITEFHTVKQFNQHTLVEAHPLTG
                     RTHQIRLHMAFLGCPIVGDLVYGRKKPSLDLERHFLHAYQISIILPGESEARTFQAEL
                     PADLQTILDQLI"
     gene            complement(7388..7915)
                     /gene="lspA"
                     /locus_tag="PLW25_04050"
     CDS             complement(7388..7915)
                     /gene="lspA"
                     /locus_tag="PLW25_04050"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOS53695.1"
                     /translation="MKHFLKKTVWLFVVAASIVTLDQVLKKIVITHLPYGEVWSPWEW
                     LTPYARIVHWSNTGVAFGMLQGMNPVFIGLAVLVSFGIVYYYPKIEKKDWMIKLALMM
                     ELGGAVGNLVDRIKYGHVIDFISVGTFPVWNIADACITVGVIILLLGVWLQEKREKNG
                     KAKTTQEQADNEVSP"
     gene            complement(7999..11148)
                     /gene="ileS"
                     /locus_tag="PLW25_04055"
     CDS             complement(7999..11148)
                     /gene="ileS"
                     /locus_tag="PLW25_04055"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOS53696.1"
                     /translation="MFKPVSPKLDITDMELKVLRYWQTHEIFHKSMSSREGGPEYVFY
                     EGPPTANGKPGVHHVLARAFKDIFPRYKTMRGYHVSRRGGWDTHGLPVEIEVEKKLGF
                     TNKQQIEDYGIGKFNDLCRESAFTYIQEWEKLTDRIGYWVDLTNAYITYTNEYIESVW
                     WILKSFWEKNLLYQGYKVVPYCPRCGTPLSDHEVAQGYRDASDPSVFVRMPLVDTPGT
                     SLLVWTTTPWTLPANVAVAAGAEIDYVTVERQMEDGSKEELILAESLVERVFAGETVQ
                     VMNCFKGKRLQGLRYQPLFTFLPAEKKAHYVVLGEFVTTEDGTGLVHIAPAFGADDMQ
                     VALEHDLPILNTVAQDGTFIPQVNPWAGIFVKDADPQITQNLKNRGLLFREETYLHTY
                     PFCWRCDTPLLYYARPTWYIRTSQYKERMVKLNEEINWYPEHIRKGRFGNWLENNVDW
                     ALGRERYWGTPLPVWQCDTCHQQECIGSIAELSDRAGRNLANANLHRPEVDKIQFACS
                     ACDGNMRRVPELIDVWFDSGSMPVSQWHYPFENVEAFKSQFPADFICEAVDQTRGWFY
                     SLHAISTLLFDEKCFKNVICLGLILDGEGQKMSKSRRNVIAPWSVIEKHGADAMRWYL
                     YTASPPGQERRFSADLVGEVLRNFTLTLWNTYSFFVTYANLDKWSPVPDEKVAYSALD
                     KWMRSRLHALIRDVTIALENYDVLGATRPVQTFVEQLSNWYLRRSRRRFWKSESDADK
                     NAAYSTLYECLTALSKLLAPTMPFIADELYLNLVGSVDKKAWESVHLAPWPEFDSETI
                     DEKLNDEMELVMKLTSLGHAARNKANRKVRQPLAEVAFSVGSQEEERVIKRNAELLAD
                     ELNVKKVRTLNAAGEAVSYTLNPLPKQLGQKYGSLYPEIRKKVLALDVEASALKLLAG
                     ESLRIEAGGNVLDILPEEVEVRLQAKEGYSVASEGAYLAALVTELTPDLISEGLAREF
                     VRRVQDLRKTADLDIADRIRLYYSATPLLAQAVETYRDYIMMETLAVAFQGGGAPEGT
                     FTVTDEFDQEKVTIHIIKA"
     gene            complement(11464..12774)
                     /locus_tag="PLW25_04060"
     CDS             complement(11464..12774)
                     /locus_tag="PLW25_04060"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOS53697.1"
                     /translation="MRAVDIIIKKRDRKEMTREEIEFFVRGIVNGEIPDYQVSAWAMA
                     VLLNGMSDQETTDLTLAMAYSGEVLDLSGIVPMEVDKHSTGGVGDKTTLVVEPLVSAC
                     GLPVGKMSGRGLGFTGGTLDKIESIPGMRTNLTQEEFKRLLRDEGLVLAGQSGDLAPA
                     DGKLYALRDVTGTVQSIPLIASSVMSKKIAGGAQGIVLDVKVGSGAFMKNLEEARQLS
                     RLMVTIGTLSGRKVVALLSDMNQPLGRAVGNALELVEAIDTLKGRGTADFTEHCLVVA
                     SHMLLIGEKAQNLDAARKMAEAALADGSGFEKLKQLVKNQGGDAAYVDDCALFPPAPI
                     IRGVQSPQKGYLSRVDALEIGEASVNMGAGRNKKEDTIDHRVGIIVHHKVGDLVHTGD
                     ELFTIHARDESSWQMAANSVLSACRWQEQPCEPLPLFYEVIKQK"
     gene            complement(12877..13653)
                     /gene="jag"
                     /locus_tag="PLW25_04065"
     CDS             complement(12877..13653)
                     /gene="jag"
                     /locus_tag="PLW25_04065"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOS53698.1"
                     /translation="MSTEKTTLEIIAPTTEEAVERGLEQLGLTEDMVEIEVLDGGSRG
                     LLGLGGRQSRVRLTVKSFETLIDEHEPIFTGDADSEEITLEPSSVHAIDLADLSEEDQ
                     QNVKSVRSVVIELLERMRVKADVSARYIEPQDEKDQRVVLVDVEGKDLSILIGRRSET
                     LNALQYITGLIVSKELGRWVPLLIDIQGYRSRRERQLRQLGRRMADQAVQTGRKQTLE
                     PMPANERRIIHLELRDHPQVFTESVGEEPYRKVTIALRNP"
     gene            complement(13682..14464)
                     /locus_tag="PLW25_04070"
     CDS             complement(13682..14464)
                     /locus_tag="PLW25_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOS53699.1"
                     /translation="MWDTIIIEPFINLLLWISKLVGNFGLALILFTILTRIVLYPLTA
                     KQIKSSAAMQELQKDKRYIEIQTKYKGDKEKLAQEQMKLYQEMKINPMASCLPTLIQF
                     PIIIGLYQAMIQTMATGPLDLLNLTRHIYPKFLSIAEVLPLNSKFLWMDIGMPERLHI
                     PGLNFGIPVLAIVVVISTYISTKIMTPPQQPGAADQGAMMSKMMNLYMPVLMGWLAWS
                     LQSGLALYFVVTNFVQIAQYAISGKVHWDNVVFWKKKPEGKK"
     gene            complement(14477..15532)
                     /locus_tag="PLW25_04075"
     CDS             complement(14477..15532)
                     /locus_tag="PLW25_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOS53700.1"
                     /translation="MKIRRIGLILLFVLLAVTLTACSRAGQSNSWSGVLVTEDYVYYA
                     GGSQVTALVADSGNVAWQYPEKGSPQRLFYAEPVLIGEQLIVVDYAHQLTCLNAKTGA
                     ETWKFTQAEGRYIDSPLVVNNLILAPNTDYSLYALDLSGNLVWEFKAGHALWARPATD
                     GTTVYFPSMDKYLYALDAATGALKWKVALDTTTISRPLLENGVLYLGNLDGTFFAFNA
                     EDGTRIWQQKVGGGVWSQPVLHDGKLYFGDQSGRINILNSENGKSVQQIETDAAIVGT
                     GALLDEGLVFGNEDGQVILVGFNGERLWTRSFDGSIYASPERLGNRLVISLNKSEKPL
                     IALDTNGNENWYYGKKK"
     gene            complement(15578..15784)
                     /gene="yidD"
                     /locus_tag="PLW25_04080"
     CDS             complement(15578..15784)
                     /gene="yidD"
                     /locus_tag="PLW25_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOS53701.1"
                     /translation="MPLLGLIRLYQWLVSPALPANTCRFYPSCSHYGYQAIYKHGAIK
                     GTILAVWRVLRCNPFNPGGFDPVP"
     gene            complement(15849..16208)
                     /gene="rnpA"
                     /locus_tag="PLW25_04085"
     CDS             complement(15849..16208)
                     /gene="rnpA"
                     /locus_tag="PLW25_04085"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOS53702.1"
                     /translation="MKRKFRLTQTNDFKRVRHTGRSYAHPLAVLVCAKGLAENSRAGI
                     IATRSVGGAVERNRVKRQLRAIIAPYLSELKDPIDFLLIARERTSQVQFQEIQAAVVE
                     LLGKAGLIGSNNYRPGR"
     gene            complement(16275..16445)
                     /gene="rpmH"
                     /locus_tag="PLW25_04090"
     CDS             complement(16275..16445)
                     /gene="rpmH"
                     /locus_tag="PLW25_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOS53703.1"
                     /translation="MTKRTYQPKIRRRVRVHGFRARMATADGRATLKRRRAKGRHHLT
                     VTMNNHVKKISW"
     gene            complement(16519..17199)
                     /locus_tag="PLW25_04095"
     CDS             complement(16519..17199)
                     /locus_tag="PLW25_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOS53704.1"
                     /translation="MFVVIAGGGRTGAQLAKSLVSAGHRVHLIESRKEILARIHKEIP
                     TESIIAGNHLDTHTMELADLIHADVFAATTPVDAENLALCYVARERYDVNRTIARVNN
                     PRNAWLFNKMFNVDVAVNQAEILAHLIEEEMSMGDMMLLLKLRRGKYSLVEEKIPVNA
                     TAVGMMIKDLKLPANCVIAGIIRDGELVIPRGVTTLAAEDELLVLTDAEGARMVAELL
                     DAPRVKNL"
     gene            complement(17201..17860)
                     /locus_tag="PLW25_04100"
     CDS             complement(17201..17860)
                     /locus_tag="PLW25_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOS53705.1"
                     /translation="MKIIVIGCGRIGADLAYNLFKRGHQVSVIDENAASFKNLPSDFE
                     GKTLEGDAINQDLLTRAGIQTCDALAAVTNNDALNVVVSHVAKNEFNVKNVVARNYDT
                     HLRSIFETFNIQVISSSSWGAQRIEELLLDAEGRTVFSAGNGEVEIYEIIIPPSWNEK
                     KVSDLVTCSKCKWLAVTRAGSAFLPDQDTVIKAGDILTVAATFEGITETRENLRKRQV
                     S"
     gene            complement(17876..>19582)
                     /locus_tag="PLW25_04105"
     CDS             complement(17876..>19582)
                     /locus_tag="PLW25_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOS53706.1"
                     /translation="LSVPIAIGICALLAILIFSYEQTVHAYPNGGGSFIVARDNIGAI
                     PAQIAAAALLTDYILTVSVSISSGVAQLISAFPLFEPYKVLIAVSFVLLIMLVNLRGV
                     KESGITFAIPTYFFLGMMYINVIVGFIRLIAGTLGSVEAPPMDLILPESALQPVTLFL
                     VLKAFASGTSAVTGVEAISDGITAFKEPRSKNAGTTLIIMALILGSMMFSITFLGNHV
                     GVMPSEEETVISQMSRTIFEGRGLLYLGTIAATTIILVMAANTAFADFPRLSAIAAAD
                     GYLPRQLTYRGSRLVYSRGIAALAIVASLLIIIFQASVSRLIPLYAIGVFLSFTISQS
                     GMARRWWKSGKLEPGVEVQERGSLLKYDSGWKHKMLINGFGALITFVVMIVFAVTKFV
                     DGAYIVVFLIPALVLGFSTIHKHYYTLAKDLSLEEYAPPGRIVHQRVLLLVSGVHKGT
                     LTALRYAKTLSDDVTAVHVSIDPLETRRMLEKWEMWGEGYRMVVIESPFRLFIEPLLE
                     YIEEIDKNRPPNEIISIIVPQFIPRRGIASLLHSRTADLLRKVLLSRKDIVIMEVPYL
                     VD"
BASE COUNT         4708 a         5456 c         4483 g         4935 t
ORIGIN      
        1 atggataatg gaaaccgttt gccggcagcg tgtctgaaga cactgacccc aacaaaaacc
       61 agcaccaaaa ccagcaggta gctgcccaag ccaaatgccc ggcggatgag agtaatccag
      121 gaattgatga gggttccccg gcttaaccct aacaaaccca gggaggtcag aatggcgagc
      181 agaatcagaa atgtgccaag caggtcccaa ccaaactggc gaaagtgagt cagaatggcg
      241 tctattcgct caaccagagg aacgaattct ttcggagcgg gattcttttt tggagggaat
      301 ttatccttcg ggctgggtct tggtgtcatt cttcgctgat caatcccagt ccaagcctgc
      361 gccggtctgc atccacgtgc aaaatcttca cttgcactct ttgacccgga gtgtaatgct
      421 cggcgatatt cattttgcgc agggtctcgg ggaacgctga aatgtggatc agcccttcaa
      481 ccccttcatc taaccttgcg aaaattccaa aacgcatgat tgatgtcagt tcagcggaaa
      541 ccacatcgcc tggtttatat atttttgaca accgctccca tgggttaggc gtaagacgct
      601 tgtgactcaa tgcgatgcgt gagttggctt cattaacttg caacacgatg actttaacct
      661 gttcgcctac cttaaaaaca tgcgatggtt tttccacccg tccccaagac aactccgaca
      721 catggatcag cccttcaacc ccgcccagat cgacaaaaac accaaagtcc gtgatattgg
      781 tcaccacacc gcaaacaatg gtgccctctg taagttgggt gaacaattcc ttgcgctttc
      841 cctcccctgc ctgagcggcc ctctcagaaa gaacaacacg ctcactctcc atatcgcatt
      901 caatgatttt tagatagaga gatgtcccca cataattcgc gagtttttgc tgacggactt
      961 catcactgac ctctgcctga aagtcaacca ggtgagagac cggcacaaac ccctgaatgc
     1021 ctgcaccctg caccagcagc ccgccccggt tatagccttg cacttcgagc agtaccactt
     1081 catcatcagc ataaaccgct ttgatgcagt cccaatcgag ttggctggtt gttttcgatt
     1141 cacagggttt gcagtttacc tctttatttt cgcgaaaaaa cttttcctcg tccgcgagaa
     1201 ctgatgccca ccagccttca tccatctctg gaatgctcgc ttcctgtttc ctttcctgta
     1261 caatttccac cataaaagcc cctccgaaca ataaaaaacg ataaacagag ccgtgcttat
     1321 gtattcctgg tgttcagctc catttgcacc agccgatccg cgattttttc gattgctact
     1381 ttctgtttcc gatagaggtc tgcttccgaa acggctaatt ttacagcaat ctcgcgtact
     1441 ttctttccct ccaggaactt catttctaaa atattataca ataaccattc gccggtgtat
     1501 tttctttctc cttctggttt gagctgttca agcgcgcttc gaaggacggt tctcaaagca
     1561 ttgacttcgt tgttgtcgtg ctttgccatg gcttctttta cgatttccag ctttagcaaa
     1621 ggactatcag ttaattttgg tcctccccaa taatgggtta atgccccttt tacccaggtg
     1681 gtcatttcct tcttttccat ccaatcggct tccatcagca taccgcgttg gtcaaatctg
     1741 ctgactgccc gaagtttttg aatcaattcc tcctgcggct ccaatctctc cagtgtgtca
     1801 aaaacctgct ccagcatgtg ccgatcgcgc aaggcgatcg cagcacgcgc cgccagccgc
     1861 ttcagcgcag cctggtcttc cgcagccaac gcggagatgt ctttgatccc cgtaatacca
     1921 atgtatccca agaggtgttc cccagaaccg ttactgttgt tgtgcaaagg aatcaggata
     1981 tctgaccggc actcaacata ttcttcatgg atatttcctg cgctttccag ataatccagc
     2041 tcttccaata gttgcgggtc aaatgaaaaa tcaggagttt tttcaaccag agtaaaacct
     2101 tcatcatttt tctccagcag ataggctcct tgcgcctgcg ctctatcgtt cacagccgca
     2161 agcaccatct caataaactg tttcaggtct ccgcgagtga taatacgctg ttccagtgtt
     2221 agcacgaaac gcaaatcctg ttcgtctcgg tcataaatca gccatctttc aaaagcgggg
     2281 aaaaacaaag taatggcgta ctcacatatc agtacagtca ctaccatcac tataggaaca
     2341 aaagcgtcat acggggtacc cagtgcaata ttcgctcgct tcaccagggt cattaaaccc
     2401 agtgtcaaaa cagcggtgat cggtcctctt aaagtccatt tgatcaagcg gctctttacc
     2461 acgcgatctg gccaaggaac cccaaagaac gccacagcgt atgccatgat aaagatacag
     2521 atatccaacc cgatcgatac tgtcgccgct aaaagccaga agagcaaagt ccattgactg
     2581 gcgaactcgc tgctgtaaag caagaaaggg aaagccccca caactggacc aatcgcgctg
     2641 attgccaggt acaacatgcg ccgtttactg gtgcgtgtga gcgtgcgctg gtaggctcgc
     2701 cgaaaattaa cccaggcgag caccaaaata atgatgtaat aaacaatgaa cagctgggta
     2761 tatattgtgg gcaagtgatg aggagcaggc ggttgatcaa gcaccaactc acccagaaac
     2821 cagggcgtaa acaggaaaaa gatcaggatg aatgaaaaaa ggtaagttgc cctgatcgcc
     2881 cacctgcggc gccagcgaga ggggcgcccg gtagtggcca acagcgcatc agaaaaattc
     2941 aggtaggcgg caggcagcag gatgattccc acccactgcg cgcgcaacca gaagtcgatc
     3001 atgtacgggt ctttgcttgt cgccccgaat gattctgtag aaaacacgat gaccaggcag
     3061 accataattg tcgcgaacgt tctggctact ctttccagca gattgaacgt caggctgtat
     3121 aacagcagcg caaaagcagt aatggcaatg ccggcggtta agatttcact tattgtcctg
     3181 gctatcgcga tgatttcatt cacccagtta ctgaccatca tcttcctact ttaattcact
     3241 caaataaaac aacaccatgt ccccattttg gtagaaattg tccacctgac cggcgtgaga
     3301 gaagcgtaat tttacggcca tctcaaccat ggcatggttt tttgccggta tttcgagcaa
     3361 catccttgtc atccccctct tcttcaccca gtctctcgcg gaaaaaacca gcgcgctggc
     3421 gataccctgg cggcggaatg gggtattgac aaccaggtcg tgtacaaccg ccaacaattt
     3481 atcgttatct tcatccaacg tcaggtaccc tgccaggctg ccttcacaac tggcaattaa
     3541 gaaaagcaaa tgccgggtcc atgtgtttct gagcagttct gccctgcgcg gatagttcaa
     3601 tcgcaatacc cggggaagtt ttacctcatt gagttcaatt gcaatccggt cttttgtggt
     3661 gctcccatcc actcgccata catgcgtggt ttcaatcgta tggtcaaatt tctccaatgt
     3721 tgaaatatcg cttgataccg cggggcgaat ttcgacttct gccattttta ctccgatgct
     3781 tttatggtca ccaggatcac acatgctggt agcgaattat tttggatgtc gttcaccaca
     3841 aggcgcagtt tgtattcacc tggaattaat gcccgggtat cccacgttcc cccaaaaggt
     3901 tcattaatga tttttgcatt tcccgcagcg atggttgtcc atgtatcact atctgaaggc
     3961 gaatactcat atttataaaa gcccaggttt tcaatattta ctgtgccttt cagttcgacc
     4021 ctccccgaga gggtatctcc gtttttcggg tctgtccaat caatctggtc aggaatacat
     4081 ccacggctaa agtaagaggt aatggtgggg atcagccctt ccccagtaac catcaatatt
     4141 ggcgctgggg tttcagttgg cggtagcgcg gtcaggtcaa tggtgggtgt gggcaattgt
     4201 tgcactcccg gtacagcagg agtgataaaa gtattgacca ggaacaatcc ggttgccagt
     4261 aatacgctga aaaccagtac agttaaagcc gagttaaata tctgttgagc atgttctttt
     4321 tccagcccga aaatactcct ggaccactcc cgccacgcca cgtatgtttt cctcgcggac
     4381 gcgaggatga tcaggccggc aaccaggaaa atgaggatct catatttttc gaggaaacgt
     4441 agaatactgc tcacgataat ttgaccaata aagatgcatt caatgctaga acctattcgc
     4501 ctgccagggt ttcaatcaca ccgcgaatga tcgtggtcag tttgggtacg atcacttttc
     4561 cggcttccag gacctcttcg tgagtggtga ccgtgctgcc atccaggttg gctttattac
     4621 tgattcccga taaacccagg acacgcatct ttgcgtgtcg tgccacaatg ttttctggta
     4681 ctgttgacat cccaaccgcg tccgccccga tgatcttgag aaaccgcagg tccgccggcg
     4741 attcaaacga agggccagcc agacaaacat aaaccccttc atgcagctta atgtgatgtt
     4801 tctgcgctac ctgcctggcg gcagcgataa agagggagtc ataaggctgg ctcatatccg
     4861 gaaaacgttc gccaaattcg ttcaaattgg gtccgcgcag agggttttgc cccgccatac
     4921 cgatgaggtt aagatggtca gtcagcagca tcacatcgcc cggtacataa tccgggttaa
     4981 tggcgccagc cgcgttggtt agaaccaggt tgcgcacgcc catgcgctgc atcacccgta
     5041 tcgggaaggt gaccctgtcc atcgagtacc cttcataata atgcaccctg ccctgcatca
     5101 ccatggccgg caccccgaaa agccgcccga aaaccaggcg cccagaatga ccctgaatgg
     5161 tggataccgg ccattcaggg atctggttaa agggaatgat tctggcgtca ttcaccgcct
     5221 ctgccaagcc ccccaacccg gatcccagta ccagcgcaac cacaggcttt tcaacgattt
     5281 gcctggttat aaaattcgca gcactatcaa tttctcttat cgtgataaaa gaatccatgc
     5341 tcacctcaat gcttgcctgc aaagtggaat acctgttttt attatatcag aatgacctta
     5401 agcgattgtc agctgtttac gcgccagaaa gcgcgctgcg gaatgcccgg attgtttcgt
     5461 caacgtcttt atctgtgatc caataatgcg tcaccaggcg aaacctgcgc tgagcaacca
     5521 cacccacttt gacgcccagc cttgccaagc gctcggcaac ctcctgagca gaaaaaatga
     5581 cctcttcctt gagcgaaaga aaaaccatat tactttgcgg catgcctatt tcaaagtgaa
     5641 tgccaggcac tgtcatcaag ccttctgcca gcagagttgc cctttggtga tcctctctta
     5701 atctggtgat catattttcg agggcaaaaa tgccggcagc ggcgataatt cctgcctgcc
     5761 gcattccccc acctaacatt ttacgcacgc gcaacccttt cttaatgaac tccttcgtcc
     5821 cacagaccac gctgcccact ggtgcgcaca gccccttgct caggcaaaag gtcacgctat
     5881 ccactggctc cgtgagagcc ttgacatcaa cattcaacgc tgctgcggca ttgaagatgc
     5941 gcgcgccatc aaggtgtaaa tggagatgat tgcggtgcgc caattctgcc acttcacggg
     6001 tatacctctc agtgagcact gttcctccgc atcgattgtg cgtattttcc agtatcacca
     6061 tgcgcgtaat ggggtcatga atattatccg ggcgtatggc gttttcaata tcttcaagca
     6121 tcaggctgcc atctgcctgg ttgggcaggg tatgcgcgaa tatgccgcca agagcggaaa
     6181 tcccgcccgc ttcataaaga aaggtgtgcc caaggttgcc cataatgacc tcatctcccc
     6241 ggttgcacac gcttaaaaca gaaagaaggt tccccattgt tccactgggc acgaacaacc
     6301 cggcttcttt gcccaataaa gcagccgaca tttcctgtaa ccggttaacg gtgggatctt
     6361 ctccatatac atcatcaccc acttcagtag ccgccatcgc ctggcgcatt tcttcagtag
     6421 gagtggtgac cgtatccgag cgtaaatcga tcaatttcat caggctctcc gcgttagata
     6481 agttgatcca ggatggtttg caaatcagcg ggtaattccg cttgaaaggt tcgggcttcg
     6541 ctttcacccg gtaaaatgat gcttatctgg taggcgtgca agaaatgccg ctccaaatca
     6601 agcgagggct tttttctgcc gtagacaagg tcacccacaa tcgggcagcc cagaaacgcc
     6661 atatgcaatc tgatttggtg cgtacgcccc gtcagggggt gtgcttctac caaggtgtgt
     6721 tggttgaatt gcttgacggt gtgaaactcc gttatggctg ctcgtccccg accatcagaa
     6781 accaccgtca tttgtttgcg atgagccgca tcccgggcga ttgccgcttc cacccggcct
     6841 gatggcgttg ggggttttcc atccaccaat gccaggtaag tttttttgac cttgcgtaaa
     6901 cggaactgtt cttggagcca gcggtgggcc tttccattct tggctacaat gatcaacccc
     6961 gaagtgtcct tatccaggcg gtgcacgatt cccggccgta cttcgccgcc aatgccctgt
     7021 aaatctccaa aatttcctaa cgccgcgtgc accagtgtcc cgctggcatg gcctggggct
     7081 ggatgaacga ccatcccggc aggtttgtta atgatgacca gatgctcgtt ttcgaagact
     7141 atatcaagcg ggatactctc cggttcaagg ttaaccggta ccacctctgg aatgcatatg
     7201 ctgactttat caccagtcag cagcgccagc cctgattttg ttgccacggt ttcattaacc
     7261 gtcacaccgc catccttaat aatcgcctgc aaccgggaac gcgactgacc cggtaattgc
     7321 cgggccagga atttatccaa cctgttctca tctccgcttt gcacgataaa ctggcagcgc
     7381 tgtgagctca agggctaacc tcattgtccg cctgctcctg cgtggttttt gctttcccgt
     7441 ttttctctcg tttttcttgc agccacacac ccagcagcag gatgataacc cccacagtaa
     7501 tgcacgcgtc agcgatgttc cagaccggga aagttccaac tgagatgaag tcaatcacat
     7561 gaccatactt gatcctatcg acaaggttgc caaccgcgcc gccgagttcc atcatcaacg
     7621 ccagtttgat catccagtct tttttttcta tcttagggta ataatagaca ataccgaatg
     7681 aaaccagaac agccagtcca ataaacaccg ggttcatgcc ctgaagcatt ccaaaagcca
     7741 caccggtatt gctccaatgc acaatccgag catagggtgt caaccactcc catggagacc
     7801 acacctcgcc ataggggagg tgggtaatta caattttctt caatacctgg tccagcgtaa
     7861 caatactggc agccacaaca aataaccaga cggttttctt taaaaagtgc ttcaattaag
     7921 gctccatttg acagtggttg aattttaccc cagaatgcag atgaaaagcc tggggctcct
     7981 ccacggagcc ccagaagttc aggctttgat gatatgtatg gttacttttt cctgatcaaa
     8041 ctcatctgtg accgtaaacg ttccctctgg cgctcccccg ccctgaaaag cgactgcgag
     8101 ggtctccatc ataatgtaat cacgataagt ttcaaccgcc tgcgccagga gaggtgtggc
     8161 gctataataa agccggatac ggtcagcgat atccagatct gctgttttac gcaagtcctg
     8221 tacccggcgc acaaattctc gcgccaaccc ttcgctgatc aagtcggggg tcaattcagt
     8281 cactaacgcc gccagataag ccccttctga cgccactgag tatccttctt tcgcctgcag
     8341 tctaacctcc acctcttcag ggagaatatc caaaacattc ccgcctgctt ctatgcgcaa
     8401 actctctcct gccagcagct tcagtgcgct ggcttccaca tccagcgcca aaaccttctt
     8461 gcggatttca ggatacaggc tgccgtattt ttgtcctaac tgcttgggca acgggtttaa
     8521 tgtataactc accgcctctc ccgcggcatt caacgtcctc acctttttta cattcaactc
     8581 atccgccaat aattcagcat tacgctttat tacccgctcc tcttcctggc tgcccacaga
     8641 aaaagccact tctgcgaggg gctgcctcac cttacggttg gctttgttgc gcgcagcgtg
     8701 ccccaaagag gtcagcttca taaccagttc catctcgtca ttcaatttct cgtcaatcgt
     8761 ctcgctgtca aattcaggcc acggcgcaag gtgtaccgat tcccatgcct ttttatccac
     8821 cgaacccacc aggttcaagt acaactcatc cgcgataaaa ggcatggttg gcgcaagcaa
     8881 cttgcttaga gcggtcaggc attcataaag agtactgtaa gctgcgttct tatccgcatc
     8941 actttcgctc ttccaaaaac gtctgcgtga gcggcgcaga taccagttag agagttgctc
     9001 cacaaatgtc tgcaccggcc gggttgcccc cagtacatca taattttcaa gcgctatggt
     9061 gacgtctctg atcaacgcgt gcagcctgga gcgcatccac ttatccaggg cgctgtaagc
     9121 caccttttcg tctggtacgg ggctccattt atccagattg gcataagtca cgaagaaaga
     9181 ataggtgttc cacaaggtca atgtgaaatt acgcagtact tcacctacca ggtctgcgga
     9241 gaaacgccgc tcctgtcctg gcgggctggc ggtataaagg taccagcgca tcgcgtcagc
     9301 gccatgcttt tcaataaccg accagggagc gatgacattc ctgcgggatt ttgacatttt
     9361 ctgcccttcg ccgtctaaga tcaaacccag gcagatcaca ttcttaaaac acttctcatc
     9421 aaataacaaa gtgctgatgg cgtgcagcga gtagaaccaa ccacgtgttt ggtcgaccgc
     9481 ctcgcaaata aaatccgctg ggaactggct cttaaacgct tcgacgttct cgaacgggta
     9541 atgccactgg ctcacaggca ttgagcctga atcaaaccaa acatcgatca actcggggac
     9601 gcgccgcatg ttcccgtcgc atgcagaaca cgcaaattgg attttgtcta cttcgggtct
     9661 atgcaggtta gcgttcgcga ggtttctgcc ggcacgatct gatagctcag caattgagcc
     9721 tatgcattcc tgttgatggc aggtgtcgca ctgccaaacc ggcagcggtg tgccccaata
     9781 gcgttcccgc cccaaagccc aatcaacgtt gttttcgagc cagttgccaa accgtccttt
     9841 acggatgtgt tcaggatacc agttaatttc ttcgtttaac ttcaccattc gttctttata
     9901 ctggctggtt cggatatacc aggttgggcg cgcgtagtat agcagcggcg tgtcgcatcg
     9961 ccagcagaag gggtaagtgt gcaaataagt ctcttctcgg aacagcaatc ccctgttttt
    10021 cagattttgg gtgatttgtg ggtcagcgtc cttgacgaaa atgccggccc agggatttac
    10081 ctgcgggatg aatgtgccgt cttgagcgac cgtgttcaaa atgggcaggt catgctcgag
    10141 cgccacctgc atatcatccg cgccaaacgc aggggcaatg tgtaccaaac cggttccgtc
    10201 ttctgttgtc acaaactcac ccagcaccac gtaatgggcc tttttttccg cgggcaggaa
    10261 tgtgaacaat ggctggtagc gcagcccctg taaacgcttc cctttaaaac agttcatcac
    10321 ctgcactgtt tcaccagcaa atacgcgttc caccagtgat tcagccagga tcaactcctc
    10381 tttggatcca tcctccattt gccgctcaac agtcacgtaa tcaatttcag cgcctgccgc
    10441 caccgctacg ttcgccggta aggtccaggg agtggtcgtc cacaccagca gggaggtacc
    10501 cggggtgtca accagcggca tacgcacgaa caccgatggg tcagaggcat cacgatagcc
    10561 ctgcgccact tcatggtctg aaagcggcgt accacaacgc gggcagtacg gcaccacctt
    10621 atacccctga taaaggaggt tcttctccca gaagctcttc agaatccacc acaccgattc
    10681 aatgtattca ttggtgtaag tgatgtaagc gttggtgagg tccacccaat agccaatgcg
    10741 gtctgtgagt ttttcccact cctggatata agtgaaggct gactcgcggc aaaggtcatt
    10801 aaacttgcca atcccgtagt cttcaatttg ttgtttattg gtgaagccta attttttctc
    10861 cacctcaatt tcgactggta gtccgtgggt gtcccatccg ccgcgtcgtg acacgtggta
    10921 tccgcgcatg gtcttgtaac gcggaaagat atccttaaag gcccgcgcca gtacgtgatg
    10981 aacccctggt ttgccatttg cggttggggg tccttcgtaa aatacatatt caggaccacc
    11041 ctcgcgggaa ctcatgcttt tatgaaatat ctcgtgtgtt tgccaatagc gtaatacttt
    11101 caattccatg tcagtgatgt ccagctttgg cgatacaggt ttaaacattc ttcctccgat
    11161 aacaaaaatc ccatccctct ccagggacgg gaaaatgggc ccgcggtacc accctgcttc
    11221 tctttcttga gcgctttgcc aggtgcattc tgtcaataga cctgatccac tgtaacggtc
    11281 ggatttccgg ctcacttact tgcacaaggc tttcaggtcg cagctccgga aggattttca
    11341 gctcattcac cagcccggct ttcacctcac ccggtctcgc tgttggttaa cgcgagctta
    11401 ctcgtttcca tcgatgcttt tcttagcgga attatctcat aaacatttca aaatatcaat
    11461 cttttatttt tgcttgatca cttcgtaaaa taatggcagc ggctcgcagg gctgctcctg
    11521 ccaacggcag gcggagagca cgctgttggc tgccatttgc catgagcttt catccctggc
    11581 gtgaatggtg aacaactcgt cacccgtatg caccaggtca cctaccttgt gatgcacaat
    11641 gatgccgacc cggtggtcaa tcgtatcttc tttcttattc cgtcctgcgc ccatgttcac
    11701 agaagcctcg ccgatctcca gcgcgtccac acggctcagg taaccctttt gtggagactg
    11761 caccccgcgg atgatcggtg cgggagggaa taaagcgcag tcatccacat atgcggcgtc
    11821 gcctccctgg ttcttcacca gttgtttcag tttttcgaac ccgctcccat ctgccagcgc
    11881 cgcttctgcc attttcctcg ccgcatccag gttttgcgct ttttcaccaa tcagcagcat
    11941 gtgtgaagcc accaccaggc aatgctcagt aaaatcggct gttccccggc ctttgagggt
    12001 gtctatggct tcaactaatt ccaaggcgtt gcctaccgcc cgacccagcg gctggttcat
    12061 atccgaaagc aacgctacca ccttgcgccc gctcaatgta ccgatggtca ccattaaacg
    12121 agaaagctgc ctggcttctt ccaggttttt cataaacgcc ccactgccca ctttcacatc
    12181 cagcacaatg ccttgagcac caccagcgat tttcttactc ataacggatg aagcgatgag
    12241 aggaattgat tgcacagtgc cggtgacatc gcgcaatgca taaagcttgc catcggccgg
    12301 cgccaggtct ccgctttgcc cagccaatac cagcccttca tcgcgcagta agcgtttgaa
    12361 ttcctcctgc gtgaggttcg tgcgcatgcc tggaattgac tcaattttat ccagcgttcc
    12421 acccgtgaat cccagccccc ggcctgacat tttgccaaca ggcagcccgc aggcagaaac
    12481 aagcggctca accaccaggg tggtcttgtc tcccactccg ccggttgagt gtttatcaac
    12541 ctccatgggt acgattcctg aaaggtcaag cacctcacct gaatacgcca ttgccagtgt
    12601 caggtccgtg gtctcctggt cgctcatccc attaagcagt accgccatcg cccaggcaga
    12661 cacctggtaa tctgggattt cgccgttaac gattccgcgc acaaaaaact caatctcttc
    12721 acgggtcatt tccttgcggt cacgtttctt gataataata tccactgctc tcatgggcgc
    12781 tccgtctcgt tgatgattgg atgattattt tactatagcc atcatcgaaa aacgccaggt
    12841 cagttgacct ggcgttcatc aatcgatatt cacggattaa gggttcctga gcgcgatcgt
    12901 caccttacgg taaggttctt ctcctacgct ttcagtgaaa acctgggggt gatcgcgcag
    12961 ttcaaggtgt atgatgcgcc tttcatttgc aggcatcggt tcaagggtct gtttgcgccc
    13021 agtctgtacc gcctggtcag ccatgcgtct gccgagctgg cgtaattgcc gttctcttcg
    13081 gctacggtac ccctggatgt cgatcagcag cggtacccaa cgccccagtt ctttgctgac
    13141 gatcagcccg gtaatatatt gcagcgcgtt aagcgtttca gaccttctac caattaaaat
    13201 actgaggtct tttccctcaa catctaccaa caccaccctt tggtcttttt catcttgcgg
    13261 ttcgatatac ctggcggaga catccgcttt cacccgcatt cgctcaagaa gctcaatcac
    13321 aactgatctc accgatttga cattctgctg gtcttcttcg ctcaggtcag ccaggtctat
    13381 ggcgtgcacc gaggagggct ctaacgtgat ttcttcactg tcagcgtccc cggtgaaaat
    13441 tggctcgtgt tcatcgatca acgtttcaaa tgatttcaca gttaggcgca ccctggattg
    13501 cctgccgcct aatcccagca gcccgcggga accgccgtcc agaacctcga tttctaccat
    13561 gtcttctgtc agacccaatt gctcgagacc tctctccacc gcctcttcag tcgttggcgc
    13621 gataatttcg agcgttgtct tttcagtgct catctgtcac ctcagtatag ttgattcagc
    13681 gttacttttt tccttctggt ttctttttcc agaaaaccac attatcccaa tgtactttgc
    13741 cagaaatcgc gtactgggcg atttggacga agttcgtcac tacaaagtaa agcgccaggc
    13801 cagattgtaa ggaccacgcc agccagccca tcaagaccgg catgtacaga ttcatcatct
    13861 tggacatcat cgccccctgg tcagcagcgc caggctgctg gggaggggtc atgattttgg
    13921 ttgagatgta ggttgaaatg accacaacaa tcgccagcac agggatacca aagttcaatc
    13981 ccggaatgtg cagacgctca ggcattccaa tatccatcca taagaatttg ctgttgagtg
    14041 gaagcacctc tgcaatgcta aggaatttcg gataaatatg tcttgtcagg ttgagcagat
    14101 ccagcggacc agtggccatg gtttggatca tcgcctggta cagcccgatg ataatgggga
    14161 attggatcaa tgtaggcagg caggacgcca tcgggttgat cttcatttcc tggtacagtt
    14221 tcatctgttc ctgggcgagc ttttccttat cacccttgta cttggtttga atctcgatgt
    14281 accgtttatc cttctgaagt tcctgcatcg cggcagagct tttaatttgc ttggccgtca
    14341 aagggtacaa cacaatcctg gtcagaatgg tgaagaggat caaggcaagg ccaaaattac
    14401 ctaccagctt ggagatccac aacaacaagt taatgaatgg ttcaataatg atggtatccc
    14461 acatggttgt ccgtccttat ttctttttgc cgtaatacca gttttcatta ccgttggtat
    14521 caagagcgat caacggtttt tcgcttttat tcaggctgat aaccaggcgg tttcccagcc
    14581 gttccgggct tgcgtagatg ctgccatcaa atgagcgagt ccataaacgt tcaccattga
    14641 aacctacaag gatgacctgg ccatcttcat tcccaaacac aaggccctca tccagcagcg
    14701 ctccagtgcc cacaattgcc gcgtcggttt caatttgctg cacgcttttc ccattctcgc
    14761 tattcaaaat attaatccgc ccggattggt ctccaaaata cagcttgccg tcatggagaa
    14821 ctggctgcga ccacactccg ccgcccactt tctgctgcca gatgcgcgta ccgtcttccg
    14881 cgttgaacgc gaaaaaggtg ccatccagat tgcccaggta gagaacgcca ttctccaaca
    14941 acgggcggga gatagtggtg gtatccaggg ctaccttcca ctttaaagcg ccggttgcgg
    15001 catccaacgc gtaaaggtat ttatccatcg agggaaaata aaccgtggtt ccatcggttg
    15061 cgggtctggc ccatagagca tgcccggcct taaactccca aacgaggttg ccagaaagat
    15121 ccagcgcgta aagagaataa tcagtattcg gcgcgagaat caagttatta acgaccagag
    15181 ggctgtcaat ataacgtccc tctgcctgtg taaatttcca ggtttctgca ccagtcttcg
    15241 cgtttagaca ggtcaactgg tgcgcataat ccaccacgat caactgctca ccaatgagca
    15301 caggttcggc atagaaaagc cgttgggggc tacccttttc tggatattgc caggcgacat
    15361 ttccactatc ggcaaccaac gcggtcacct ggcttccgcc ggcataatac acgtagtctt
    15421 cggtcaccaa cacaccagac cagctgttag actgaccggc gcgtgagcat gccgtgagtg
    15481 ttactgccag cagcacaaat agcaggatca aaccaattct tcgaattttc atgctttttt
    15541 cgttccctat tattaagaag atatccttag accgaggtca cgggaccggg tcaaatccac
    15601 ctggattaaa aggattacaa cgtaaaacac gccagacagc caggatcgta cctttaatgg
    15661 cgccatgttt ataaatcgcc tgatagccgt aatgcgaaca ggaaggataa aaacggcagg
    15721 tattggcagg caacgctgga gaaaccagcc attgatacag cctgatcaac cctaacagag
    15781 gcatgcgggg tatattaacc aatcgaaatg gaagatcgcg cagacgtggc tcagccacat
    15841 gaacgtgttc atcttcctgg gcgataattg tttgagccaa tcaatcctgc ttttcccagc
    15901 agttcaacaa ctgctgcttg aatttcctga aattgcactt gtgaggttct ttcacgcgcg
    15961 atgagtagaa agtcaatagg gtctttgagt tcagagagat atggtgcaat gatagcccgt
    16021 aactgccgct tcacccggtt gcgctcaacc gctccgccta ctgaccgtgt cgcgatgatg
    16081 cctgcccgtg aattctctgc caatcctttt gcacaaacga ggactgcaag cgggtgcgca
    16141 tacgatcgac ctgtgtggcg cacccgcttg aagtcatttg tctgggttaa tcggaacttc
    16201 cgtttcaccc gatgtccagt ccgaaatcct gaaaagcatc ttgtggatac accgttcccg
    16261 gccgttcaga aaaatcacca gctgatcttc ttgacatggt tgttcattgt aaccgtcagg
    16321 tgatgacggc ccttcgcgcg ccttcttttt aatgtcgcgc gtccatccgc tgtagccatg
    16381 cgcgcgcgaa agccatgcac acgaacacgg cgtcggattt tcggttggta agtcctcttg
    16441 gtcatgccac ttcctctcaa ttcattacag aatgcttaaa ttcgcaaact tggattatac
    16501 ctcaacctcg ttattcggtc aaaggttttt cacgcgcggc gcatcgagca gttccgccac
    16561 catcctggca ccttctgcgt cggttaagac cagcaattcg tcctcagcag ccagagtggt
    16621 cactccccgt ggaatcacaa gctcaccatc ccggataatt cccgcgatga cacagttggc
    16681 gggtaatttc aggtccttga tcatcatgcc taccgcggtt gcattgacag ggattttttc
    16741 ttctaccagc gagtattttc ctcggcgcag cttgagcaat aacatcatat cgcccattga
    16801 catttcttct tcgatcaggt gagccaaaat ctccgcctgg ttcaccgcca catccacatt
    16861 gaacatcttg ttgaacaacc acgcgttacg cgggttattc accctggcaa tggttcggtt
    16921 cacgtcgtat ctttctcttg ccacgtaaca tagagcgagg ttttcggcat ccacaggagt
    16981 ggtggctgca aaaacatccg cgtggatcag gtccgctaac tccatcgtgt gggtgtccaa
    17041 atggttgcca gcgatgatgg attcagtggg gatttcttta tgaatgcgcg caaggatctc
    17101 ttttcgcgat tcgatcaaat gcactcgatg ccccgcgctg accaacgatt tcgctaattg
    17161 cgctccagtt cgtccgccgc ctgcaatcac aacaaacata ttaactcacc tgcctttttc
    17221 tgaggttctc acgcgtttct gtaattcctt caaacgtggc tgccacggtt aatatatccc
    17281 ccgccttaat gaccgtatcc tggtctggta agaatgcgct gcccgcccta gtaaccgcca
    17341 gccatttgca tttgctgcag gttaccagat cagagacttt tttctcattc cagctcggcg
    17401 gaatgataat ttcgtaaatc tcaacctcgc cgttgcccgc cgaaaaaacc gttcgccctt
    17461 ctgcgtcaag tagaagctct tcaatacgct gggctcccca acttgaggag ctgataacct
    17521 ggatattgaa tgtttcgaaa atggaacgca aatgcgtatc ataattcctg gcaacaacgt
    17581 tttttacatt aaattcgttt ttcgccacat ggctcaccac cacgttaagc gcatcattat
    17641 tcgtgacagc tgccagcgca tcgcaggttt gaattcccgc gcgcgtcaga agatcctgat
    17701 tgatcgcgtc accttcaaga gtctttcctt caaaatcaga agggaggttc ttaaaggaag
    17761 ccgcgttttc atcgataact gaaacctggt gtcccctttt aaaaagattg taagccagat
    17821 cagccccaat ccttccgcac ccaatgacaa tgattttcat aattttctaa ccctttcaat
    17881 caaccaggta tggcacttcc atgatcacaa tatccttacg tgataataac accttacgca
    17941 gtaaatccgc agtgcgcgaa tgtaaaaggc tggcaattcc acgccggggg atgaactgcg
    18001 gcacgatgat gctgatgatc tcgttcggcg gtcggttctt gtcgatttcc tcaatgtatt
    18061 ccaacaatgg ttcaatgaac aatctgaatg gcgactcaat tacaaccatg cggtagcctt
    18121 ccccccacat ctcccacttt tccagcatgc gcctggtttc cagcggatcg atggacacat
    18181 gaacagcggt cacatcatca gaaagcgtct tggcgtagcg caacgctgtg agcgtccctt
    18241 tatgcactcc gctcaccagc aacagcacgc gttggtgtac aatgcgccct ggtggggcgt
    18301 attcttccag cgaaaggtct tttgccaggg tgtaataatg cttatggatg gtggaaaaac
    18361 ccaatactaa cgccgggatg aggaaaacaa cgatataagc gccatctaca aacttggtga
    18421 cagcaaaaac gatcatgacc acgaatgtga tgagcgcgcc gaacccattg atgagcattt
    18481 tgtgtttcca gccgctgtca tacttgagca gcgaaccacg ttcctgcact tccacgccag
    18541 gttcgagctt gcctgatttc caccagcgcc gcgccatacc gctttgcgat atggtgaatg
    18601 agagaaacac gccgatcgcg tagagcggaa tcagccttga aacgctcgcc tgaaatatga
    18661 tgatcagcaa agaagccaca atggccagcg cggcaattcc tcttgaatac accaggcggc
    18721 tgccgcggta ggtcaattgc ctgggtaaat atccgtctgc tgccgcaatg gcgcttaaac
    18781 gcggaaaatc agcaaaggca gtgttggctg ccatcaccaa aataatggtg gtagcagcga
    18841 tagtgccaag gtagagcagc cctctgcctt caaagatggt tcgagacatt tgtgagatga
    18901 cggtttcttc ttcagaaggc atcaccccca cgtggttccc caaaaaagtg atagaaaaca
    18961 tcatcgatcc caaaatcagc gccatgatga tcagcgtggt gcctgcgttc ttgctgcgcg
    19021 gttctttaaa cgccgtgatg ccatctgaaa tcgcctctac accagtcacc gctgaggttc
    19081 cgctggcgaa agccttaagg acaagaaaga gcgtcaccgg ctgcaaggca gattcaggca
    19141 aaatcagatc cattgggggg gcttccacgc ttcccagcgt gccggcaatc aggcggatga
    19201 acccgacaat tacgttgata tacatcatcc cgaggaaaaa gtacgtggga atcgcgaagg
    19261 taattccaga ttccttcacc ccccgcaggt tcaccagcat gatcaagagc acaaaactga
    19321 ccgctatcag caccttatag ggctcgaaca acgggaaggc agagattaac tgcgccacac
    19381 cggatgagat agacacagaa accgtgagga tataatcagt tagcagcgcc gccgccgcga
    19441 tttgcgcagg tattgcgccg atattatccc gcgccacgat gaaggagcct ccgccgtttg
    19501 gataggcgtg gaccgtctgt tcgtatgaaa agatgaggat ggccaggagc gcgcaaattc
    19561 caatcgcgat cggtactgaa ag
//
LOCUS       DAOSPM010000029        19563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2782_length_19563_cov_12.355546, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000029 DAOSPM010000000
VERSION     DAOSPM010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19563
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2782_length_19563_cov_12.355546"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PLW25_04110"
     CDS             complement(<1..1001)
                     /locus_tag="PLW25_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53707.1"
                     /translation="MYNLELHSMYLLGGKAQSTLPGLIALSPPRKCARGREFDQLVAH
                     VDLRGKTSITPEAMQEWMLKKGALFFSTPGSTTNAMRMMADSINNELFDRNLKKAADS
                     LKVNGTLSMVVLRKDTIYSLVIGQGRIFILEDEAVIELADHDNSPGGLGMNQSLNYLF
                     SQSNVSSDCKVLLSPLPSPQWTPAFLAGGGRLPADVLGRRLLNQCPQELRGMLLLLSE
                     GSGKITNSTLIQAVPTDGAVTSSAPAMPDTKPAIQPALIDLREVAKRPAQAEPAGDPH
                     PAMKNTADSRLPAAIKSGLAKGQKTPIQRKNVSARRRRQTGLQIDPEKIKARVGSAV"
     gene            1132..3612
                     /locus_tag="PLW25_04115"
     CDS             1132..3612
                     /locus_tag="PLW25_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOS53708.1"
                     /translation="MMSSDNSNYFIGRVYDPKTKGPTQEDVLLDSANLTTHCVVTGMT
                     GSGKTGLCIGLLEEAALKKTPAIIIDPKGDLTNLVLHFPNQQPADFQPWIDPEAARRE
                     GKSVEQMAEETASRWSKGLAEWGIGKEKLQALAEAAEYTIYSPGSTSGIPVNILSSFE
                     APEIPFNENAEVLRERISTTVTAVLGLIGLSNIDPLRSREHILLSSLIENAWIHDTSL
                     SLTELILQVQNPPMDRLGAFPMESFFPEKNRFELALLLNNFLASPSFQVWQQGQPLDI
                     GALLYTADGGPRHSIFYLAHLSENERMFFVTLLFAAVESWMRGQRGTGNLRAILYFDE
                     IMGYLPPASSPNPNPPSKVVLLRLLKQARAFGVGLVIATQNPLDLDYKALSNAGTWMI
                     GRLQTSLDKQRLMDGLSSASGSTDIRTIDNLISGLEKRVFMLHSVYRSKPVLFNTRWA
                     MNFLAGPMTRDQLAAANALAGVNAHATGAPTETVKPAQKTTSTVREGSGLTSSQPAIP
                     GDIAEYFYPNNLGISEAAATAQFSGALDSEGLAYRPGIIAQAEVRFVSRQYNLEQINK
                     TCAILEDPGSGLVRWDELVSSEVDKDRLEMHPLPKAKFHPVPSWLSDARKRDELKKDF
                     LDWIYRSGGLKILANNKLQLFGTPGIPEAEFRRSVQDAVDKTVEEEIEKASKAADSKI
                     AAMERKIQSQELEVKAAESTVNQRRLETMATGGAVVIGMLTGRKRSISSTLSKNRMAS
                     AAKDKLEAKKTTLAQYTEQLAELKKAKEELVAKVKEKWDGIADEISEITIKPTKSDIF
                     PDIFAVVWLPYYIVEQEGKKIELAGFKR"
     gene            complement(3659..4267)
                     /gene="recR"
                     /locus_tag="PLW25_04120"
     CDS             complement(3659..4267)
                     /gene="recR"
                     /locus_tag="PLW25_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOS53709.1"
                     /translation="MPILPVPIQEMIGALERLPGIGPKSASRLAFFLLRAPQDLVQQL
                     TSALTNLKSATGTCQLCANITLADQELCEVCRDPQRADGTICVVEEPLDVLALERTGG
                     FHGRYHVLQGVLSPIEGVGPDDLTIRGLMERVRAGEAKEVILATNPSMEGDATALYLR
                     QQLLALGVRVTRLARGLPVGGDLEYADQHTLLRALAGRQEMT"
     gene            complement(4280..4615)
                     /locus_tag="PLW25_04125"
     CDS             complement(4280..4615)
                     /locus_tag="PLW25_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOS53710.1"
                     /translation="MAKGFNRPAGGQGSGGMMAQIKRLQEQMETAQQQLALETVTSSA
                     GGGAVKVTMTGDQRCTEVTIDPDLLKDADAEMLQDLVLSAVNLALDDSRKLAAEKLGP
                     LAGGMPGLF"
     gene            complement(4633..6309)
                     /gene="dnaX"
                     /locus_tag="PLW25_04130"
     CDS             complement(4633..6309)
                     /gene="dnaX"
                     /locus_tag="PLW25_04130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOS53711.1"
                     /translation="MTQAFYRKWRPRRWDQVVAQDHVVQTLKNAIKADRVGHAYLFSG
                     PRGTGKTTTARLLAKAVNCLNENPAERPCDSCSHCLAVNENRFLDLIEIDAASNTSVE
                     DVRDLRDRINYSPTDGKYKVYIIDEVHMLSTAAFNALLKTLEEPPPHAIFILATTEVH
                     KIPATVLSRCQRHEFRRIPVAETVKALREICKEEQIETDEEALLLIARQSTGALRDAI
                     SLLDQLASTGKHIDLALAQTVLGTATNQLVFDLIEGLLKNDPALGMQTIHKALDGGSD
                     PRQYARQVVEYLRNLLLMKMGNEKEIEATREVKNRIREHSDRVEIDALMEWIGLFNAA
                     VSDLRTTWQPSLALEVAFARALNPAGAERVPVLETGTEKQQSRSTPAAEKIATREMAA
                     AEKKAEKKNEPPAAEEVADTPAPLPEEPAARVQIEQPGKVHGKEMAGAGSAELTTQDL
                     LASWSAIRAEVKLRRIQAEALLNSQKLLQVKNGILVLGFPSEVLRSKMEIPENIEVTR
                     RVIQNLLQVDLPIQCVLVTEKGAISSSNDSEPNGLVDAAINLGGKLIHKD"
     gene            complement(6404..6886)
                     /locus_tag="PLW25_04135"
     CDS             complement(6404..6886)
                     /locus_tag="PLW25_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOS53712.1"
                     /translation="MRKILPYLLLNFVVSALAVWIVLMVWEANHQIPNIPAAEQPVTR
                     ETKSPTITQPPLDEKTITIQLVMGSGDIRSERVQLVSASASPVNLQGWELVDEDKNRY
                     QFPSVTLFPGAAIELYTKGGVNTAGELFWNREEPVYTSGERILLKDTSGNIRSEYQVP
                     "
     gene            6954..7448
                     /locus_tag="PLW25_04140"
     CDS             6954..7448
                     /locus_tag="PLW25_04140"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOS53713.1"
                     /translation="MKKILIDGHNLIPKVGGISLSDPEDEAKLTARVSEYCRLAKVKA
                     ELFFDGAPPGKNATSRLGLINVHHVRQGTTADAAIIQYLHREGYNARNLLVVSSDHEV
                     QNKARMLHAEVMSSDEFARQMRAVFSSPQASMNIKETPPSEEEVEEMLKLMTENREEN
                     STTT"
     gene            complement(7799..7885)
                     /locus_tag="PLW25_04145"
     tRNA            complement(7799..7885)
                     /locus_tag="PLW25_04145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7847..7849),aa:Ser,seq:gga)
     gene            8060..8482
                     /locus_tag="PLW25_04150"
     CDS             8060..8482
                     /locus_tag="PLW25_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53714.1"
                     /translation="MSSIVPDLELPILVVDDAHWQKVSSTGEEGEEYSIFGRDGFRLS
                     TKGYEFTIPSGVDFIAPNIIQLVIGKDQLYATAYEPDCTLYTIDPANLVPMYGSRRFT
                     GFQKGQKLIIAIGHLSPPGHDLPQPRFIVLWAGVVNIL"
     gene            8556..8632
                     /locus_tag="PLW25_04155"
     tRNA            8556..8632
                     /locus_tag="PLW25_04155"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8590..8592,aa:Val,seq:gac)
     gene            8818..9744
                     /locus_tag="PLW25_04160"
     CDS             8818..9744
                     /locus_tag="PLW25_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOS53715.1"
                     /translation="MSRYLIILNPIAGKGKALRSLTHIKESLAQYNLEFDIKVTEYPG
                     HGTEIARKAAEEKWDYVIAAGGDGTANEVLNGLMQNRAAGGFLPVMGILPVGRGNDFA
                     FGANVPTELDEACTLLASLPRKWIDIGHVSGGDAPQGMYFGNGIGVGFDAMVGFMAAK
                     MKLTGMLSYLVAAIKTIFIYFKPPNVEIKFADKTLTLPALMVSIMNGRRMGGGFMMAP
                     SSSITDGQLNICMVREVPQFSMFGLMAKFIKGTHESDPAVSVARADRITIIAKKGALP
                     VHADGNTVGEACHEITVELLPHSLEIITRLEN"
     gene            9744..10418
                     /locus_tag="PLW25_04165"
     CDS             9744..10418
                     /locus_tag="PLW25_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOS53716.1"
                     /translation="MLTAAHINPPIKTILRAFMRIDDSGIHSVPMSGPLIMATNHINW
                     LDAPVGFSHLHPRKITAFAKIETWDNYWLRLLFNAWEAIPISRGEVDFNAFRKAEEAL
                     AAHKIIAIAPEGTRSKTGKLNTGYPGIVFLALRTNAPIMPFVFYGNENIQLNLRKYRR
                     TPMVLKAGAPFRLKAQSSNPDKLMRRELTDAIMYEIARLLPEEYRGKYAEIDPGYRRH
                     LEFIDL"
     gene            10539..11156
                     /gene="infC"
                     /locus_tag="PLW25_04170"
     CDS             10539..11156
                     /gene="infC"
                     /locus_tag="PLW25_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOS53717.1"
                     /translation="MSTQNFRVNELIKVAEVRLIGPQNENIGVVPIQKALQIAREAEL
                     DLVEVAPLSEPPVCRVMDFGKFLYERTKKEKEARKAQTKIEIKEIRLRPKTNEHHRGF
                     KTRDARRWLLEGNKVKVTIRFRGREITYPEIALEDLKEIAEELADIATIEQAPNMEGR
                     TMGMVLSPSKPGKKKPSPAPEKKDAAEDKANEVPPGEPQKTAEKA"
     gene            11270..11497
                     /locus_tag="PLW25_04175"
     CDS             11270..11497
                     /locus_tag="PLW25_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HOS53718.1"
                     /translation="MPHKMKTGKFKLKTHKATSKRFRLTGSGVLLRTKGGKSHLRRRT
                     SDRTKALFTEMVPVQGDKIIQRVKRLNPNMK"
     gene            11596..11946
                     /gene="rplT"
                     /locus_tag="PLW25_04180"
     CDS             11596..11946
                     /gene="rplT"
                     /locus_tag="PLW25_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOS53719.1"
                     /translation="MARVRSGFTSHQRHKRTLKFTQGQFGSRHRLFRRANEARLKSMW
                     YASRDRRMRKRDLRRLWITRINAGSRQNGITYSQLIHGLRKANILLNRKMLADLAARD
                     PQAFSAVVEKAKAA"
     gene            12024..12827
                     /locus_tag="PLW25_04185"
     CDS             12024..12827
                     /locus_tag="PLW25_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOS53720.1"
                     /translation="MRLALEVITSFKNPKVQRLRSLISQRSYRDETGQFVIEGVRLVE
                     EAFRRHFLPQSVYFSVNLSARGIQLVDQLRQAGVEVFEVSGDILNRLSDTETSQGILA
                     VLPQELISLTGVAEPVLIIDQVRDPGNLGTILRSAAAAAAGAVLLAPGSVDPFMPKVV
                     RAGMGAHFHLPICQTSWDGIEDYCKRQAQPPLQVLLADAGSGLPIWQADLTRPLALVI
                     GGEAEGAGKEARALSDAAIHIPMPGNFESLNAGVAASLLLYEVLRQRSA"
     gene            12824..14041
                     /locus_tag="PLW25_04190"
     CDS             12824..14041
                     /locus_tag="PLW25_04190"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HOS53721.1"
                     /translation="MKVRSITSFYDPACPQAERQLKRLAEVSEALREAIQQKLEVQST
                     RLGTSPFSCYLNDRPPAEVISCATQLEQKAQSLGWQYLTMGPALPEHPWAYKLIPQLI
                     RSTKNVFFSAVITANHTLYPGAVLAGAQIIHQTAQNSTDGFANLRFAALASVEPWTPF
                     LPAAYHQAGSAPAISIAVECADAVNDAFSQDLTLEECRQSLLDSLQNASSQLEEVILP
                     IIHSGGIRFKGFDFSPAPFPEDWCSLAGAAERLGLAHIGGLGTLPAIALIADTLDRGN
                     WRKAGFNGMMLPLLEDSILAKRAAEGQLNVKDLLLYSTLCGTGLDTIPLPGDTSIEQL
                     RSVLMDIAAISIRLDKPLTARLMPIPGKSAGDFTDFHFPFFANSRVLSLEGQPLHRFF
                     TSQDPIHICPRRR"
     gene            complement(14058..>15883)
                     /locus_tag="PLW25_04195"
     CDS             complement(14058..>15883)
                     /locus_tag="PLW25_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOS53722.1"
                     /translation="TLDPHLQLEADRIVREQVEALADRHVTDGALVAIDPESGEILAM
                     TGSADFYNEDIAGQINMALQPRQPGSSIKPLTYAAAFEKGWTPATVLWDVPSEFPPSG
                     DPNDTRDPYIPVNYDGKFHGPVSVRSALANSYNVPAVKALEFVGIYDNPATVEADGLI
                     AFARRLGISTLVRNDYGLSLTLGGGEVTLFDMTSAFSVFANNGRRVVPVAITRIEDHK
                     GKIIYESNPREPEQVMRAEHAYLITSILSDAMARAPMFGSDSILNLPFPAAVKTGTTN
                     EYRDNWTIGYTPDLVTGVWVGNADNSPMVGTSGVSGAAPIWNAFMNSAIPYLTGNAPA
                     NFTRPEGIVEKEICVYSGAEPSENCTERRRELFAFDQPPPSSEDDLWKLLKVDTWTNL
                     KASSACEGFAETKLVLNVSDKWAKKWIDDTDQGRAWLEKINFSPPVIYVPSRECRADD
                     PRPTLLFVGLNDGQTITTDKLDIYAVVDATKNFKEYYLEYGEGKDPHKWTTLVEPGGA
                     TSADPQKLLTWDLLKVNNGVVTLRLFMLSTKNGHAEKLLRLNLQVPTPTPTNTPTPTE
                     TPTITPTPTSTATPTPTLTLTPMPLFPTLDAFLQTLLPPGN"
     assembly_gap    15884..15983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15984..17623)
                     /locus_tag="PLW25_04200"
     CDS             complement(<15984..17623)
                     /locus_tag="PLW25_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOS53723.1"
                     /translation="MTDDVNTPADESAVPQKPIDPSLPNIGRFQNLPNPEEPPGYTWQ
                     FIQDRSHSQATVPVTPSNEEQVADSKTEPSSPHPQDEQNSQGPSGDTEPHRLRLRTSD
                     TGGEKAESEHFEDLLTVPSVSLPSITRKEEGYPPRKVIPPAGGRDGLPRKVDEVDLSA
                     TIATPAALQVNRTGEKAARKLPAAPPARAKIPAMNRRKPTRPRQQKKPGFDYGGCLVK
                     VVVILLFMLVVGVIFAGIVFVYQYYTIASTLPSVEDIRGHASQFETTRFFDRNGQVLY
                     EMIDPNAGRRTYTPLEDISPYVLAATIATEDKEFYNHPGFDLLALVRAMVTNYTSGEV
                     VSGASTITQQLARTLFFTPEERVEISYRRKAKEIILSSEITRRYTKDEILELYLNEIN
                     YGNLAYGIEAAAETYFNTTAKELTLAQAAFLAGLPQAPSVYDIFSNRDMTLNRNKQVL
                     TAMYNVSKEKDCIYVSNSPQRICIEAQQAADAYIWIDNYEFKLKANPMVYPHWVTYIR
                     YLLETMYDPQMIYRSGFRVFTTLDPHLQLEADRIVREQV"
     gene            17709..>19563
                     /locus_tag="PLW25_04205"
     CDS             17709..>19563
                     /locus_tag="PLW25_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53724.1"
                     /translation="MLPSVIPQAICRFLCVDMRFGKPGILDDQCWAIGTELAEPKALS
                     LLTTYGLRASRMHVFPQFSMENLVIQDPARFSAYPVVSFSSTCLVKFTFSPFPSIDVE
                     YRVWVPHPQVVVGQMNCLNTGQALLKVVVDWRVHLQPLQGGSPMKHAQAGLNTIMQGE
                     CANLRPVFYLTGGAMASLTDFPGLGNQMLLMPGTSRQVTWALASLTSVESSTQQARQF
                     SSSALELEQIKIEMADKGSKVHCTSAQAALDDKLQHAQDRTHQLLMPPLQKHTQITYV
                     ANRSPDLGYHVSESILDAQPEWNGQTLPEIYLLAHNLLPGRPEALKGLLQNFLNLQQP
                     NGRIDLRASVNHTLTGHSSLPMLASLVSELQPYLDDFTWLRGIYPQLLAFLKTWVVLD
                     DSGSLVLSALTHPLQLGFNDHSPDTQADLVELWFRLAHPRNLFLVSLLYREVSELLRI
                     GRVIEMNDALDWLEQIRARLAEAGTDLLKPTDRMNHPSAPGTTRLVLASFHNDGFPRF
                     KQNLPHPGKICLRLDYQGRLPAELNGCLTGFAAGTYLEKRITAADFQPVGSSFCYVPR
                     EEFEFIESISVKRLPEGARGEISLVDQNSPSVFELMACYAGFLSEKDSRRIT"
BASE COUNT         4522 a         5351 c         4999 g         4591 t
ORIGIN      
        1 tctaccgcgc tgcctacccg ggctttaatt ttctcagggt ctatctgcag cccggtctgc
       61 ctgcgccgcc tggctgatac gttcttacgc tgaatagggg ttttttgtcc tttcgccagc
      121 ccgctcttta tcgcggcggg taaccgcgaa tccgctgtat tcttcatagc cgggtgagga
      181 tctccggctg gttctgcctg cgcagggcgc ttggctacct ccctcaggtc gatcaatgcc
      241 ggctgtatgg ctggcttcgt gtcaggcatc gcaggcgcgg aggaagtgac cgcgccatcc
      301 gtaggaaccg cctggatgag agtactgttg gtaatcttgc cgctgccttc tgacagaagc
      361 agcagcatcc ctcgcaattc ttgcgggcat tgatttaaca gcctgcgccc aagcacatcc
      421 gccggcagcc tgcctcctcc agccaggaac gcaggcgtcc actgggggct gggcagcgga
      481 gacaacagaa ccttgcagtc actactgaca ttgctttgcg aaaacaggta attcaatgac
      541 tggttcatcc ccagcccgcc cggggaatta tcatggtctg ccaactcgat gacggcttcg
      601 tcttccagga taaaaatgcg cccctgcccg ataaccagcg aatagatcgt gtctttgcgc
      661 aacaccacca tagaaagcgt gccgttcact ttcaggctat cagcagcttt ttttagattc
      721 ctgtcaaaca attcattgtt gattgaatca gccatcatgc gcatggcgtt ggtggtggag
      781 cccggagtgc taaaaaacaa ggctcctttc ttcagcatcc attcctgcat ggcttcaggc
      841 gtgatactgg ttttacctct taagtcaacg tgcgccacca gctggtcaaa ttcacgccct
      901 ctagcgcatt tgcgcggggg agacagcgca atgagtcccg gcagggtcga ttgcgcctta
      961 ccgccaagca ggtacataga atggagttcg aggttataca aaatggttcc ttgcccttgt
     1021 cgatcgattg ttgaataacg gatacaagaa acgggccaaa agagccgggg ttaccataaa
     1081 tcgattcccc gctttttcat cttataatag aaagaaaaac aggtggtggg aatgatgtct
     1141 tcagataatt caaattactt catcggaaga gtgtacgacc caaaaaccaa gggaccaacg
     1201 caagaggacg tgctgctgga ctccgcgaac ctgacaaccc attgcgttgt caccggtatg
     1261 actggttcag ggaagacagg tttatgcatc ggtttgctcg aagaagccgc cttaaagaaa
     1321 accccggcga ttatcatcga cccgaaaggc gacctgacca atctggtgct gcattttccc
     1381 aaccagcagc cggctgattt ccaaccctgg atcgaccctg aagccgcccg gcgtgagggt
     1441 aaaagcgttg agcaaatggc tgaagaaacc gccagccgct ggagcaaagg tttggcagaa
     1501 tggggaatcg gcaaagaaaa attgcaggca ttggctgaag ccgctgaata caccatttat
     1561 tctcctggct ccacatccgg aatcccggtg aacatcctct catcatttga agcgcccgag
     1621 atcccgttca atgaaaacgc cgaggtattg cgcgagcgca tctccaccac ggttactgcg
     1681 gtgctggggt taatcggtct gagcaacatt gacccgctgc gctctcgcga gcacatcttg
     1741 ctctcgagcc tgattgaaaa cgcctggatc catgacacct ccctatcgct caccgaattg
     1801 atcttgcaag tgcaaaatcc gccaatggat cgactcggcg ccttcccaat ggaatctttc
     1861 ttccccgaga aaaaccgctt cgaactggcg cttctattga ataacttcct cgcttcgcct
     1921 tccttccagg tctggcagca aggtcaacca ctggatatag gcgcattgct ttacactgct
     1981 gatggcggtc cacgccactc cattttctac ctggcgcacc tgagcgagaa tgaacgcatg
     2041 ttctttgtga cgctgctttt cgccgccgtc gaaagctgga tgcgcggaca gcgcggtaca
     2101 ggaaatctgc gtgccatcct gtactttgat gaaatcatgg gttacctgcc cccagcgtct
     2161 agcccaaacc ccaacccacc ctccaaggtt gtgttgctgc gcttgctcaa acaggcgcgc
     2221 gctttcgggg taggactggt gattgccacg cagaatccgc ttgacctgga ttacaaagcg
     2281 ctttcgaacg ccggcacctg gatgattggc cggttacaaa ccagcctgga caaacaacgc
     2341 ctgatggacg ggctgtcttc cgcctctggc agcacggaca tccgtaccat tgacaatctc
     2401 atctccgggc tggagaaacg ggtattcatg ctgcacagcg tctaccgctc caagccagtg
     2461 ctctttaata cgcgctgggc gatgaatttc ctggctggac ccatgacgcg cgaccagctg
     2521 gcagcagcca acgcgcttgc tggtgtcaac gcccacgcaa ctggcgcgcc aactgaaacg
     2581 gtcaaaccgg cgcaaaaaac gacctcaaca gtccgtgaag gctctggtct aacctccagc
     2641 caaccggcga tccccggtga tattgcggaa tacttttacc ctaacaacct gggcatcagc
     2701 gaagccgcgg caacagccca attctctggc gcgctcgact ctgaggggct ggcttatcga
     2761 ccaggcatca tagcgcaggc tgaagtgcgg tttgtttcca ggcagtacaa ccttgaacag
     2821 atcaacaaaa cttgcgcgat tctcgaagac ccaggcagcg ggctcgtccg ctgggacgag
     2881 cttgtttcca gtgaggtcga caaagacagg ctggaaatgc acccgctgcc caaggctaag
     2941 tttcatcctg ttccatcctg gctgtctgat gcgcgtaaaa gggatgaact taaaaaggac
     3001 tttctggatt ggatttaccg cagcggcggt ttaaagatat tggccaataa taagcttcaa
     3061 ctcttcggta cacccggtat acctgaagcc gagttccgcc gcagtgttca agacgcggtg
     3121 gacaaaacgg ttgaggaaga aattgaaaaa gccagcaagg cggctgacag caagattgcc
     3181 gccatggagc gcaagataca atcgcaagag ctggaggtaa aggcagctga atccactgtg
     3241 aatcaacgcc gccttgaaac gatggccacc ggtggcgcgg tggtgattgg catgctcact
     3301 gggcgaaaac gcagcatcag cagcacgctt tcaaaaaatc gaatggcttc tgcagccaaa
     3361 gacaaactgg aagcaaaaaa gaccacactg gcacaataca cggaacaatt ggcagaattg
     3421 aaaaaagcca aagaagagct ggtcgccaag gtgaaagaaa aatgggatgg gatcgcagat
     3481 gaaatcagtg aaatcaccat aaaaccaacc aagagcgata tcttccccga cattttcgcg
     3541 gttgtttggc ttccttatta catcgtagaa caagaaggaa agaaaatcga gctagccggg
     3601 tttaagagat aagtatccct ttttcaacca aaaggacctg agctgagccc aggtcctttt
     3661 acgtcatctc ctgccggcca gccagcgcgc gcagcagcgt gtgctggtcg gcgtattcca
     3721 ggtctccacc aactggcaga ccgcgagcca ggcgggtaac gcgcaccccc agcgccagca
     3781 gttgttgtct caaatagagc gcggtggcgt cgccttccat gctggggttg gtcgccagga
     3841 tcacctcttt cgcttctccg gcgcgtacac gttccatgag tccgcgaatg gtaaggtcat
     3901 cagggcccac tccctcgata ggtgacagca cgccctgcag cacgtgataa cgcccgtgga
     3961 atccccccgt gcgttccaac gccagcacat ccaagggctc ttctaccacg caaatggtgc
     4021 cgtcagcgcg ctgcgggtcg cggcacactt cgcacagttc ctggtcggcc agggtgatat
     4081 tcgcacacag ctggcaagta ccagtagccg atttaagatt ggtgagcgcg ctggtcaatt
     4141 gctgcaccag gtcctgcggg gctcgcagca aaaagaacgc caggcgcgag gctgatttgg
     4201 ggccaatacc gggcagcctt tccaacgcgc cgatcatttc ctggatgggg acaggtaaaa
     4261 tgggcatggg ctgcctggtt tagaacaggc ctggcatgcc gccagccagc gggccgagtt
     4321 tttccgctgc cagcttacgc gaatcatcca acgccaggtt cactgctgaa agcaccaggt
     4381 cttgcagcat ttctgcgtcg gcgtctttta acaggtcagg gtcaatggtc acctcggtgc
     4441 agcgttggtc gccagtcatg gtcaccttca ccgcgccgcc ccctgcggaa gaggtaacgg
     4501 tttccaacgc cagttgctgt tgtgccgttt ccatttgttc ttgcaagcgc ttgatctgtg
     4561 ccatcatccc accactgcct tgcccgcctg ccggtcgatt gaatcctttt gccataatga
     4621 ctccctctac tttcagtctt tgtgtatcag ctttccgccc aggttgatgg ctgcatccac
     4681 cagcccattt ggttctgagt cgttactgga tgaaatagcc cctttttcag ttaccagcac
     4741 gcattgaatg ggcaaatcca cctgcagcag gttctgaatg acccttcgtg tgacctcgat
     4801 attttcaggg atttccattt tggaacggag cacctcgctg ggaaaaccca ataccaagat
     4861 cccgtttttt acctgcagca atttctgcga gttgagaagc gcttcagcct gaatacggcg
     4921 caacttgacc tcggcgcgga tggcactcca ggaagccagt aagtcctggg tggtcagctc
     4981 tgcgcttccc gcgcctgcca tttcctttcc atggactttc cctggttgtt caatttgcac
     5041 tcgcgctgcg ggctcttcag gcaaaggggc tggcgtgtct gcaacctcct cagccgcagg
     5101 gggttcattt tttttctcag ccttcttctc cgcagcggcc atttcgcggg ttgcaatttt
     5161 ttctgctgct ggcgttgacc gggattgctg tttttctgtg cccgtctcca acaccggcac
     5221 ccgctcagcg ccagcggggt tcagcgcgcg cgcgaaggct acctccagcg ccaggctggg
     5281 ctgccaggtg gtgcgcaagt cgctgacagc cgcgttaaac aggccaatcc actccatcaa
     5341 cgcgtctatc tcaacccggt cactgtgttc gcgtatccgg tttttcacct cccgggtggc
     5401 ttcgatttct ttctcgttgc ccattttcat cagtaacagg ttgcgcaggt attccaccac
     5461 ctggcgcgca tactgacgtg ggtcgctgcc accatccagc gccttgtgaa tggtctgcat
     5521 gcccaacgcg ggatcgttct taagcaaccc ctcgatcagg tcaaagacca attggttggt
     5581 agcggtgccc agaaccgtct gcgccaaagc caggtcaata tgcttgccgg tgctggcaag
     5641 ctgatcaagc agcgagatgg catcccgtaa ggcgccggtg gactggcgcg cgatcaacag
     5701 cagcgcttct tcatcagttt cgatctgttc ttctttgcaa atctcccgca gcgccttcac
     5761 cgtttctgcc accgggatgc gcctgaattc gtggcgctga caacgtgaaa gcactgtggc
     5821 cggtatttta tgcacttctg tggtggccaa aatgaagatg gcgtgtggag gaggctcttc
     5881 aagcgttttc aataacgcgt tgaaagccgc ggtggagagc atatggactt catcaataat
     5941 gtagaccttg tatttaccgt ctgtgggcga gtaattgatc ctgtcacgta agtcacggac
     6001 gtcttcaacg ctggtattcg aagcggcatc gatttcaatc aaatccagaa aacggttttc
     6061 gtttaccgcc aggcagtggc tgcagctatc gcaggggcgt tcagccgggt tttcattgag
     6121 gcagttgact gccttagcca gcagccgcgc agttgtggtt tttccggtgc cacgcgggcc
     6181 agaaaaaagg tacgcatgcc cgactctgtc cgctttgatg gcattcttga gagtctgcac
     6241 cacatggtcc tgcgccacta cctgatccca cctgcgtgga cgccatttac ggtaaaaagc
     6301 ctgggtcata tgtatttatc cgatctttgc tgtctttcca ttattttaca gatattccga
     6361 ttttacccga aaccctgtct cttccttctt tactgaaggt cttttaaggt acctggtact
     6421 cggagcggat attcccgctg gtgtctttga gaaggattct ctccccgctg gtataaaccg
     6481 gttcttcgcg attccagaat aattcgccag cggtattcac tcctcctttg gtgtaaagct
     6541 ctatcgcggc gccgggaaaa agcgtcacgg aagggaactg gtagcgattt ttgtcttcat
     6601 ccaccaattc ccaaccctgc aggttcactg gagatgccga agcgctgacc agctgcaccc
     6661 tttcgctgcg gatatctccg ctgcccatca ccagttggat tgtgatggtc ttttcatcca
     6721 atggaggttg ggtgattgtg ggtgattttg tttcacgggt aactggctgt tcagccgcgg
     6781 ggatattcgg aatttgatga ttcgcttccc acaccatcag cacgatccac accgccaggg
     6841 cagagacgac aaaattaagt aacaggtaag ggagaatttt ccgcataatc caatcgtttt
     6901 tctgaaagaa tcatagcata atttcaaagt aaaatgtctg gtacaagagt gaaatgaaga
     6961 agattttaat tgacgggcac aacctcattc caaaagtcgg cggcatcagc ctttcggacc
     7021 ctgaagacga agccaaactc accgccaggg tcagcgaata ttgccgcctg gcgaaggtaa
     7081 aagccgagct gttctttgat ggcgctccac ccgggaaaaa cgctacttca cgtttggggt
     7141 tgatcaatgt tcaccacgtg cggcagggaa ccaccgccga cgcagccatc attcaatacc
     7201 tccacagaga aggctacaat gcccgtaacc tgctggtggt ctcttcggac cacgaagtgc
     7261 agaataaagc ccgtatgctg cacgcagaag tgatgagctc tgacgaattt gccaggcaaa
     7321 tgcgcgcggt tttttccagc ccacaggcga gcatgaatat caaagagaca cctccatctg
     7381 aagaagaagt tgaagagatg ctcaaattga tgacagaaaa ccgcgaagaa aattcaacaa
     7441 ccacttgatt ttaagacaat ttaggtatac aatgtcttta taacacactt ttattggtag
     7501 caagaccagt gtgttccacc aaaggtcaaa aagaccaaag gtggggttcc ctccctatag
     7561 ataggcagac atgtcccccc catgcctgcc tatcgcttta agcagaggtt cactggattc
     7621 atatagccca tatgattaaa acgaactacc cgccgggtct gcctgcgcgg gagttgatag
     7681 gcgtcactgg cccttctatt attcacttca gtctatgcgc cctggaagcc gaacgggttt
     7741 agtgaggcgg agagggctgc gctcttcgcg ccctggaagc cgaacgggtt tagtgaggcg
     7801 gagagggtgg gattcgaacc cacgatacct tgcggtatac ccgctttcca agcgggcgcg
     7861 ttaggccaga ctgcgctacc tctccgtgca tgaagcctta agcgccaata ttataccatt
     7921 tgaaatgaga agtgccagga tttcattttt cgtgctcctc tttgaggttc ctttttaaaa
     7981 tgtcctctat aattaaacaa gaaaagacag gcaggctgtc accatccatc ctctttcaat
     8041 aaggagcttg ttgcggtaaa tgtcttccat cgtacccgac ctggaattac ccatactggt
     8101 tgtggatgac gcccactggc agaaagtctc ttcaaccggg gaagaaggcg aagagtactc
     8161 catcttcggc cgtgatggat tccggctttc aaccaagggg tatgagttca ccattccgtc
     8221 tggggttgac ttcattgctc ccaacatcat ccaattggtg atcggcaaag accagctata
     8281 cgcaacagcc tacgagccgg attgcacctt gtacaccatt gaccccgcca acctggttcc
     8341 tatgtatggc tcaaggcgct tcaccggttt tcagaaagga cagaagttga tcatcgccat
     8401 tggtcatctg tctccgccag gtcatgatct accgcaaccc aggttcatcg ttttatgggc
     8461 cggggtggtg aacatccttt aaactaatcg gtcgcgccat cagctcacca atcgctattg
     8521 tcaatcaatg aaaatccatt ataataagca gcaccgggcg gttagctcag ttggttagag
     8581 cgttgcgttg acatcgcaaa ggtcgtaggt tcgagtccta taccgcccac cacaaaacac
     8641 caaaacgggg gtgttttttt taattgatgc acgattggct tccttctatc atcgatcgca
     8701 aggaaaaatc gcatcaatcg tgggattgga gtgttaacaa ggtacaacaa aggcggtatc
     8761 attgagcgca gttgacagaa accattcact tttgtttttt tagaaaaccg gagccatatg
     8821 tcacgttacc tgatcatcct taacccaatc gccggaaagg gcaaagcctt acgctccctc
     8881 acgcacatta aagagtcgct tgcgcagtat aaccttgagt tcgatatcaa ggtgactgaa
     8941 tatcctggtc acggcactga aattgcccgc aaggccgcag aagaaaaatg ggattacgtc
     9001 atcgctgctg gcggcgacgg caccgcaaac gaagtgctca acgggttgat gcaaaaccgc
     9061 gccgctggag ggtttttgcc agtgatgggt atcctcccgg ttggccgtgg caatgatttc
     9121 gccttcgggg ccaatgttcc cactgaatta gatgaagcct gcacattgct ggcatctctc
     9181 ccgcgaaaat ggatcgatat cggccatgtc agcgggggtg acgcccccca aggcatgtac
     9241 tttggcaatg gcatcggcgt aggttttgac gcgatggtgg gcttcatggc cgccaaaatg
     9301 aagttaacag gcatgctttc ttacctggtt gcagccataa agacgatttt tatttatttc
     9361 aaaccaccca acgtcgagat taagttcgca gataagacct tgacgcttcc ggctttaatg
     9421 gtttccatca tgaacggcag gcgtatgggc ggcggtttta tgatggcgcc ctcctcttcc
     9481 atcacagatg gtcaattaaa tatctgcatg gtgcgcgaag tacctcagtt ctccatgttc
     9541 ggactcatgg ccaagttcat caaaggcaca catgagtctg acccagccgt cagcgtcgcc
     9601 agggcagata gaatcaccat aatcgcaaaa aaaggtgccc tgcccgtcca tgcagatggc
     9661 aacacagtcg gcgaagcatg ccatgaaata acggtggaac tcttgcccca ttcactagaa
     9721 atcatcactc ggcttgagaa ttaatgctca ccgctgcgca catcaatccc cccattaaaa
     9781 ccatcctgcg cgccttcatg cgaattgatg acagcgggat ccactccgtg cccatgagcg
     9841 ggccactgat catggccacc aatcacatca actggcttga cgcgccggta gggttcagcc
     9901 atctgcaccc gcgtaagata accgctttcg ccaaaatcga aacctgggac aattattggc
     9961 tgcggctgct ctttaacgcc tgggaagcca tcccaatcag ccgtggcgag gttgatttta
    10021 acgcgtttcg caaggctgaa gaagcgttgg ctgcgcataa aatcatcgcc attgccccag
    10081 aaggcacccg ctcaaagact gggaaactca ataccggtta cccaggaatt gtatttttag
    10141 cgctgcgcac caacgcgccg atcatgcctt ttgttttcta cggcaacgaa aatatccaac
    10201 ttaacctgcg caagtatcgg cgtacgccga tggtgcttaa ggccggtgca ccattccggt
    10261 taaaggcgca gagcagcaac cctgataagc ttatgcgccg ggaattgacc gacgccatca
    10321 tgtacgaaat cgcccgcttg cttccggaag agtatcgcgg aaaatacgcg gaaattgacc
    10381 ctggttatcg ccggcacctg gaatttatcg acctgtaaac gtgaaaattg tcctgccaac
    10441 ccggttgact agtcattcaa aattatggta ttatcaaaat cattttttgc ccgttaacgg
    10501 aacgggcacg tcatcaatct atctaacagg agaagaccat cagtacccaa aatttccgtg
    10561 taaatgagtt gatcaaggtc gctgaagttc gtttgattgg accccaaaat gaaaatatcg
    10621 gggtcgtgcc tatccagaaa gcgttacaaa tcgcgcgtga agctgagctc gacctggtgg
    10681 aggtagcacc gctctctgag ccaccggtct gccgtgtgat ggattttggg aaattccttt
    10741 acgaacgaac caagaaggag aaggaagccc gtaaagccca aaccaagatc gagatcaaag
    10801 aaattcgttt gcgcccaaaa accaatgaac atcaccgcgg atttaaaacc cgcgatgcca
    10861 ggcgctggct gttggaaggc aacaaagtaa aagtgaccat ccgtttcaga ggtcgcgaga
    10921 tcacctaccc ggaaattgcg ctggaagacc tgaaagaaat cgcggaagaa ctggccgaca
    10981 tcgccaccat tgaacaggcg cctaatatgg aaggacgaac tatgggtatg gtgctgtcgc
    11041 catccaaacc cggcaagaaa aaaccttctc ctgctccgga aaagaaagac gccgccgagg
    11101 ataaggcgaa tgaggttccg ccaggagaac ctcagaagac cgctgaaaaa gcctgagttt
    11161 ttcgcaccga ccgctatcac gacggtgaaa attccccgga ataattgatt tctggtataa
    11221 ttcgcgttcg cgctcaaagc acgcgtaatt tgtttgagag gagtaagctg tgccacacaa
    11281 gatgaaaacc ggtaagttca agctgaagac ccataaagcg acatccaagc gatttcgcct
    11341 gacgggttca ggcgtcttgt tgcgcaccaa aggcggaaaa agccacctca ggcgccgcac
    11401 ttctgaccgc accaaggcat tgttcactga gatggtcccc gttcaaggtg ataagatcat
    11461 ccagcgtgtc aaacgtctca accccaacat gaagtaggga gaggcgctga cctgtcgtat
    11521 ccgcatcagt gcggctgttg ggtgttcaca acgggtcaca agaccggcgc ccaggggctc
    11581 gcaaaggagt aaagaatggc tcgtgtaaga agtggcttta ccagtcacca acgtcataaa
    11641 agaacactga aattcaccca ggggcagttc ggcagccggc atcgcctgtt tcgtcgcgcc
    11701 aacgaagccc gtctgaaaag catgtggtac gcctcgcgtg accgccgcat gcgcaaacgc
    11761 gatctgcgcc gtttatggat tacccgcatt aacgcaggtt cccggcaaaa cggcatcact
    11821 tacagccagt tgatccatgg tctgcgcaag gccaacatcc tgctcaaccg caagatgctc
    11881 gctgacctcg ctgcgcggga cccccaggct ttcagcgctg tggtggaaaa agccaaggcg
    11941 gcgtaaacct tccatattca actgcgggat ggctgacccc atcccgtttt gtttttaacc
    12001 tgcattcgag tagaatttat tgaatgagac ttgctttgga agtgatcacc tcgttcaaaa
    12061 acccaaaagt ccagcgcctg cgcagcttga tctcgcagcg ttcttatcgt gatgaaaccg
    12121 gtcaattcgt cattgaaggc gtgcgattgg tcgaagaagc cttcaggcgt catttcctgc
    12181 cccagtcagt ttacttcagt gtgaatctct cggcgcgggg gatacaactg gtggatcaat
    12241 tacggcaggc cggagtagag gtctttgaag tgagcggtga tattctcaac cggctttctg
    12301 acaccgaaac ctcccagggg atcctcgctg ttctcccgca agagttgata tccctcacag
    12361 gcgtggcaga acctgtgctc atcattgacc aggtgcgcga tccaggcaac ctgggaacca
    12421 ttctacgcag cgccgccgca gccgcggcgg gagcggtgtt gttggccccc ggtagcgtgg
    12481 acccctttat gccaaaggtg gtgcgcgcag gcatgggagc gcattttcat ctgcccatct
    12541 gccagacctc ctgggatgga atcgaagact actgcaagcg gcaggcgcag ccgccgttgc
    12601 aggtgctgct ggcagacgct ggcagcggtt tgcccatatg gcaggctgac ctcacccgac
    12661 cgctggcgtt ggtcattggg ggtgaggctg aaggcgctgg aaaggaagcg cgtgcgcttt
    12721 cagatgccgc cattcacatc cccatgcccg gcaactttga atcgcttaac gctggtgtgg
    12781 ctgcctctct attgctatac gaagttttac gccagagaag cgcatgaagg tccgttctat
    12841 caccagtttc tacgaccccg cttgccccca ggcagaacga cagctaaagc gcctggcaga
    12901 ggttagcgaa gcgctcaggg aggctatcca gcaaaagctg gaagtgcagt ccacccgcct
    12961 gggcacctcg ccattttcct gctacctcaa tgaccgtccg cccgcggagg ttatctcctg
    13021 cgctacacaa ttggaacaaa aggcgcaatc actgggttgg caatatctta caatgggtcc
    13081 ggcactgcca gaacatccct gggcatacaa gctcatccct caattgatcc gctcaacgaa
    13141 aaacgttttt tttagcgcgg tcattaccgc gaaccacacg ctttatcccg gcgcagtttt
    13201 agcgggggcg cagatcattc accaaaccgc gcaaaattcc accgatggct tcgccaacct
    13261 tcgcttcgct gccctcgcca gcgtggaacc ctggacgcct tttctacctg ctgcatacca
    13321 ccaggctggt tcagccccag ccatctcaat cgctgtggaa tgcgcggacg cggtaaacga
    13381 cgctttttcg caagacctca ccctggagga atgcaggcag tctcttcttg actcgttaca
    13441 aaacgcttcc agccagttgg aggaggtcat tctacccatc atccacagcg gcggaatccg
    13501 attcaaaggg ttcgatttct ctccagcgcc tttcccagaa gactggtgtt cactggcagg
    13561 cgcggcggaa cgcctgggtc tggcgcatat tggcgggttg ggcacgctgc ctgcgattgc
    13621 cctcatcgca gatacattag atagaggtaa ctggcgtaag gcaggtttta atggcatgat
    13681 gctgccgttg ctggaagact ccatcctggc aaaaagggcg gctgagggac aactaaacgt
    13741 caaagacctg ctgctttatt caaccctctg cggtaccggc ctggatacca tccccctgcc
    13801 gggcgatact tccattgagc agttgcggtc agtattgatg gacatcgctg ctatatccat
    13861 ccggcttgac aaaccgctca ccgccaggct catgcccatc cccggaaaat cggccgggga
    13921 tttcactgat tttcacttcc ccttttttgc caacagcaga gtgctctcgt tggaagggca
    13981 accactgcac cgttttttta catcccaaga tcccattcac atctgcccgc ggcgaagata
    14041 agcgacaccg tatctcatca attcccgggc ggtaacagcg tctgcaggaa tgcatcaaga
    14101 gttgggaaca atggcatggg agttaacgta agcgtaggcg tgggagttgc cgtactcgta
    14161 ggggtcggtg taatggtagg cgtctcggtc ggcgtgggcg tattggtagg tgtgggagtg
    14221 ggcacctgga ggttgagccg aagcagtttt tcggcgtgtc cgtttttggt ggagagcatg
    14281 aacagacgca gcgtcaccac cccattattg accttgagca ggtcccaggt gagcaacttc
    14341 tgcgggtcgg cagaagttgc tccgccgggt tccaccagcg ttgtccactt gtgcggatcc
    14401 ttcccctccc cgtactccag gtaatattct ttaaagttct tggtggcgtc cacaactgcg
    14461 taaatgtcta atttatccgt ggtgatggtt tgcccgtcat tcaatcccac gaacaataaa
    14521 gtggggcggg ggtcatccgc tcggcattcc cttgaaggca cgtagataac cggcggagag
    14581 aagttgattt tctcaagcca cgctcttccc tggtctgtgt cgtcgatcca ttttttcgcc
    14641 cacttgtcgc ttacattcaa caccagtttg gtttcagcga agccttcgca ggcgcttgag
    14701 gctttcaggt ttgtccaggt atccaccttc aacagcttcc acaggtcatc ttcgctcgaa
    14761 ggcggaggtt gatcaaaagc aaacagctct ctcctgcgct cagtgcaatt ttccgatggt
    14821 tctgcgcctg aatagacgca aatctctttt tccacgatgc cttctggccg ggtgaaattg
    14881 gcaggcgcgt tgccagtgag gtaaggtatt gccgaattca taaaggcatt ccaaatgggc
    14941 gccgcgccag atacaccaga agtgcccacc ataggagaat tatccgcgtt gcccacccat
    15001 actccagtca ccaggtcagg ggtgtagcca atggtccagt tatcgcggta ttcattggtg
    15061 gtgcccgttt taaccgccgc cgggaacggc aggtttaaga tcgaatccga gccaaacatc
    15121 ggcgcgcgcg ccatggcatc tgaaaggatg gaagtgatca aatacgcgtg ttctgcgcgc
    15181 atcacctgtt caggttctcg cgggttcgat tcataaatga tctttccctt atgatcctca
    15241 atacgggtga tggcaaccgg cactactctt ctgccgttgt tcgcgaacac gctgaaagcg
    15301 ctggtcatat cgaacagggt tacttcaccg ccacccagcg tcagtgatag cccataatca
    15361 ttgcgtacca gtgtgctgat gcccagtcgg cgggcaaaag caatcaatcc atccgcttca
    15421 actgttgcag gattgtcata aattcccaca aattccagtg ccttgaccgc cgggacgtta
    15481 taagaattcg ccagagctga ccttactgaa acaggtccat ggaatttgcc atcgtaattg
    15541 accggtatat aagggtcacg tgtgtcgttc gggtcgccag aaggtggaaa ttcactcggc
    15601 acgtcccata aaacagtagc cggcgtccac cctttttcaa aagcggcggc ataagtgaga
    15661 ggtttgatgg atgaacccgg ttggcgtggt tgcagcgcca tattgatctg cccggcaatg
    15721 tcttcgttgt aaaaatccgc tgaaccggtc atggcgagta tctccccgct ttccgggtcg
    15781 atcgccacca acgcgccatc tgtcacatgg cggtctgcca gcgcttccac ctgttctctg
    15841 acaatacgat ctgcctcaag ctgtaagtgc gggtcgagcg tggnnnnnnn nnnnnnnnnn
    15901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15961 nnnnnnnnnn nnnnnnnnnn nnnacctgtt ctctgacaat acgatctgcc tcaagctgta
    16021 agtgcgggtc gagcgtggtg aataccctga accctgaacg gtagatcatt tgcgggtcat
    16081 acatggtttc aagcagatag cgtatgtagg tgacccaatg cgggtatacc attgggtttg
    16141 ccttcaactt aaactcataa ttgtcgatcc agatataggc gtcagctgcc tgttgcgcct
    16201 ctatacagat acgctgcggg ctgttgctca catagataca gtctttctct ttactgacgt
    16261 tgtacatggc ggtaagaacc tgcttattgc ggttcaaggt catgtcgcga ttcgagaaaa
    16321 tatcatacac acttggcgcc tgcggcaacc cggccaaaaa ggctgcttga gccagggtaa
    16381 gttctttcgc tgtggtgttg aaatacgtct ctgcggccgc ctcaataccg tacgccaggt
    16441 tgccgtaatt aatctcgttc aggtaaagtt caaggatctc atcctttgtg tagcgcctgg
    16501 tgatttctga agacagaatg atttctttcg ccttgcgccg gtaggagatt tcaacccgtt
    16561 cttcgggggt aaagaataag gttctcgcca gttgttgggt gatggtggaa gcgccagaaa
    16621 caacctctcc gctggtgtaa ttggtaacca tcgccctgac cagcgccaac aggtcaaaac
    16681 cagggtgatt atagaattcc ttatcttccg tagcgatggt tgccgccaac acatacggcg
    16741 aaatatcttc aagcggggtg taggttctac gccctgcgtt cggatcgatc atttcataga
    16801 gaacctgccc gttgcgatca aaaaagcgcg tagtttcaaa ttgagacgcg tgcccgcgaa
    16861 tatcctccac actgggcagg gtggaagcga tggtgtagta ttgatataca aagacaatac
    16921 cagcaaagat aacacccacc acaagcataa acaacagaat aacaaccacc ttgaccaggc
    16981 agccgccata atcaaagccc ggtttttttt gctgccttgg acgggtgggt tttctcctgt
    17041 tcattgcggg gatcttcgcc cgggcaggcg gcgcggcagg cagttttcgc gccgctttct
    17101 ctccagttct attcacctgg agcgcggcag gggtggcaat agtcgcggat aaatccactt
    17161 catccacctt gcgcggcaat ccatcgcgcc cccctgctgg cggaatgacc ttgcggggag
    17221 gataaccttc ttcttttctc gttattgagg gtaacgaaac gctaggtact gtgagcaggt
    17281 cttcaaaatg ttcactttca gccttttccc cgcctgtatc ggaagttctc agtctcaggc
    17341 gatggggttc tgtatcaccg cttggcccct gagagttctg ttcatcctga gggtgaggtg
    17401 aagatggctc cgtcttgcta tcagccactt gttcctcgtt ggaaggcgtt accggcacgg
    17461 tcgcctgaga atggctgcga tcttgtatga attgccaggt gtagcccggc ggttcttcag
    17521 gattcggcag gttctggaaa cgaccaatgt tcggcaagga agggtcgatg ggtttttggg
    17581 gtaccgcgga ttcatccgcc ggcgtattca catcatctgt catttacacc tttatcctgt
    17641 acagccatgg cttgattcta ctataattcc attgacctcg tgggcgtgta ctcatacaat
    17701 aagatttaat gctaccctca gtcataccac aagcgatttg ccgctttctc tgcgtggata
    17761 tgcgctttgg aaaacccggt attctggatg atcaatgctg ggcgattgga acagagctgg
    17821 cagaaccaaa ggcgttatca ctgctcacaa cttacgggtt acgcgcctcg cgcatgcacg
    17881 tttttccaca attctccatg gaaaacctgg tgattcaaga cccagccagg ttcagcgcat
    17941 acccggtggt ttccttttca tccacttgtt tggttaaatt taccttctct ccttttccat
    18001 ccatagacgt tgaataccgc gtctgggttc ctcacccgca ggtggtggtt ggtcaaatga
    18061 actgcctaaa cacggggcaa gctttgctca aagtagtggt tgattggcgc gttcatctgc
    18121 agccattgca aggcggcagc cccatgaaac acgcgcaggc aggcttgaat accatcatgc
    18181 agggagaatg cgccaacctt cgcccggttt tctaccttac cggtggagca atggcctctc
    18241 tgaccgattt tccagggttg ggcaatcaaa tgctcctgat gccaggcacc agccgccagg
    18301 tcacgtgggc gttggcttca ctcacctctg ttgaatcctc cacacaacag gcgcgtcaat
    18361 tctcttccag tgcgcttgag cttgaacaaa tcaaaataga aatggccgat aaaggttcga
    18421 aagttcattg cacctctgcc caagctgcgc ttgatgataa actgcagcat gcgcaggatc
    18481 gaactcacca gttgctcatg cccccgctgc aaaagcacac ccagatcacc tacgtggcca
    18541 accgctcacc agatctcggc taccacgttt ctgaaagtat ccttgacgca cagccagagt
    18601 ggaacggtca gaccctgccc gaaatctacc tgctcgctca caatctcctg ccaggccgcc
    18661 ctgaagcgct caaagggctg ttgcaaaatt tcctcaacct tcagcaaccc aacggtcgga
    18721 ttgatctccg cgccagcgtg aaccatacgc ttactggtca ttcatcatta cccatgctgg
    18781 cctcgctcgt atctgagctg cagccctacc tggatgattt cacctggctg aggggtatct
    18841 acccccagct gctcgctttt ttaaaaacct gggtggtatt agatgactcg gggagcctgg
    18901 ttctcagcgc cttgacccat ccgctgcaac ttggatttaa tgatcattca cccgatactc
    18961 aggcagacct ggttgaactg tggttcaggc ttgcgcatcc tcgcaacctt tttctggtgt
    19021 cgctgcttta ccgtgaggta tctgagctac tgcgcattgg ccgcgtgata gaaatgaatg
    19081 atgcgcttga ttggctggaa caaatccgcg cccggttggc agaagcagga acagatttgt
    19141 tgaaacccac ggacagaatg aaccacccgt ctgccccggg aaccaccagg ctggttcttg
    19201 ccagtttcca caatgatggc ttcccccggt tcaaacaaaa tctacctcac cccgggaaga
    19261 tttgcctgcg gctcgattac cagggaaggc ttcctgccga gttgaacggt tgtctaaccg
    19321 gttttgcagc aggcacctac ttagagaaga gaatcactgc cgctgatttt cagccagtcg
    19381 gttcatcgtt ttgttacgtt ccgcgcgaag aatttgaatt cattgaatcg ataagcgtca
    19441 agaggctgcc agagggggcg cgcggagaga tcagcctggt ggaccaaaat tccccaagcg
    19501 tttttgagct tatggcctgt tatgctggtt ttctcagtga aaaagactcc cgcagaataa
    19561 cct
//
LOCUS       DAOSPM010000030        19472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2805_length_19472_cov_15.898697, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000030 DAOSPM010000000
VERSION     DAOSPM010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19472
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_19472_cov_15.898697"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..432
                     /locus_tag="PLW25_04210"
     CDS             28..432
                     /locus_tag="PLW25_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HOS53725.1"
                     /translation="MPSIIDIEGIGSVYQKKLKAIGVATTDKLLELGATPKGRKELAE
                     KTGTGEALILEWVNHSDLYRIKGVGSEYSDLLEEAGVDTVVELSKRVAKNLYEKMVEV
                     NAAKKLVRKLPVEKQVADWIEQAKKLPRKVSY"
     gene            513..1043
                     /gene="yjjX"
                     /locus_tag="PLW25_04215"
     CDS             513..1043
                     /gene="yjjX"
                     /locus_tag="PLW25_04215"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0046128 - purine ribonucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HOS53726.1"
                     /translation="MKLVYLASENPVKIAAVEEGFRRVFTGEEFSVRSVSLSLNLPAQ
                     PKTDDETLFCAEQRAANARETAPDGDYWVGIEGGTSERGGEMGTFAWVVILDSLRRGI
                     GRSGEFFLPKILADLVRQGMELGDADDLVFARQNSKQKNGAVGLLTADAIDRLELYIP
                     AVIFALIPFKNPLLFP"
     gene            complement(1074..1967)
                     /locus_tag="PLW25_04220"
     CDS             complement(1074..1967)
                     /locus_tag="PLW25_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOS53727.1"
                     /translation="MPRTATTLISLLTCLFLVSCAAVNSSPIPTVAATDTSTPHPTST
                     PVPQATRTPTPLVCTATQGSILNVDIPTQKLNRPINTNIYLPPCYDPKKEGGYPWLIM
                     LHGQAATNAQWLELGLTNAADDLITSKEIQPLVIVMPFEVTWTPGPRESQFDEALMED
                     VLPYIEENYAVCKERTCRAIGGLSRGGNWAVHLGFVSPEVFGIVGAHSTPLFYGELWN
                     IQAAAGLPPESIPLVLVDAGDKDAEKEKVQEFVTALKDAGVPYQFFEFEGRHEPSYWS
                     AHVADYLRWYAQAFTRLQSEK"
     gene            2042..2117
                     /locus_tag="PLW25_04225"
     tRNA            2042..2117
                     /locus_tag="PLW25_04225"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2075..2077,aa:Pro,seq:tgg)
     gene            complement(2302..3396)
                     /locus_tag="PLW25_04230"
     CDS             complement(2302..3396)
                     /locus_tag="PLW25_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53728.1"
                     /translation="MTPRFIRNVLLKGLLLFALIHLAYALIPSDGLGRISAYNSLLRG
                     RERFPFGETPREAYNFSLYNFEAMFAAHRLDGTPKAADEFRVLVIGDSATWGTLLKPE
                     ETLAGQLDAAGLISADGRRVRVYNLGYPTLSLVKDLMVLERAMSYQPDLILWRVTLES
                     FPRDRQLESPIVAHNPQRVRQLIERYQLAITMPAGEPTFWQRTLIGRRRELADLLRLQ
                     LYGVMWTATGIDQLYPPSYPPAGRDLEADESFHHWQPPAFPEDGLALDLLQAGMKIAG
                     EVPVVLVNEPILVSNGENSDIRYNFYYPRWAYDLYRQTLAEASSANGWKYFDLWDLVP
                     QEHFTNSAIHLDAQGEEMLARRLIDAGLIQ"
     gene            complement(3393..4841)
                     /locus_tag="PLW25_04235"
     CDS             complement(3393..4841)
                     /locus_tag="PLW25_04235"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOS53729.1"
                     /translation="MTLLRILVLIGISLGVLVVCSIRRARRWRVVLLLVSSVVIIFWL
                     QPILPIRGLDFWLPCASLSLAGLGWALTSLPEERQRREILVTLGIVTATVLVIALTRY
                     LGSGGLLSASRPPQTLTVGIVVLVNLGLLLMAARISHRFKPALTLAIILVLLLFLVLK
                     LPALTLTTSALLRGFSGQSRELASALDVRWLGFSYIAFRLIHTLRDRQAGRLPQMALR
                     EYLIYILFFPAISAGPIDRSERFIKDLRGEFIPGALDFGAGSTRLMLGLFKKFVLADT
                     LSIVSLNASNAAQVDAAGWLWLMLLAYSLQIYLDFSGYTDIALGMARFMGIRLPENFR
                     APYLKSNLTRFWDNWHITLTQWMRAYFFFPVTRFLRNRARWMPAWLVILVTQLSTMVL
                     IGLWHGITMNFLLWGAWHGLGLFVQNRWSAGTQHLAARWQAKTGLSRAIHVLNTLLTF
                     IYVTLGWVWFTLPEPGLSTRVLVRLFGFGGGL"
     gene            complement(4992..5951)
                     /locus_tag="PLW25_04240"
     CDS             complement(4992..5951)
                     /locus_tag="PLW25_04240"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOS53730.1"
                     /translation="MRAKVSETSAFRGLLLVIITLALCGCAEAPTATERVATFSPFVE
                     DEVRSSSQAAPTSNPTILSPATHQPDTPIPTPARTQMALDDGAIMLFIPKGDFLMGSL
                     KGEGEKDEYPQHTVQLNAFWIDQTEVTNRMYRACVADGVCNEPRRSSSFTREEYYGNP
                     QYLDYPVVYVNWFDAGRYCRWAGKRLPSEAEWEKAARGSEGFIYPWGNIEPGTLHANY
                     DLLVGDTTETGSYPAGASPYGALDMAGNVMEWVLDWYGEYTTSPYKTYDPSGPENGAH
                     RIARGGSYLFSPFLIRSAERYWVSPYYADDDLGFRCAFSAVDQ"
     gene            complement(5936..6319)
                     /locus_tag="PLW25_04245"
     CDS             complement(5936..6319)
                     /locus_tag="PLW25_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53731.1"
                     /translation="MKRWKTVLLLLGFCLFLVSCSGSNSPADVVVAYYNALVAGDASS
                     LSQLSCADWEQQSKLMLDSFTGVKARVDNIVCSELENDGSSAAVNCKGKIIATYGTED
                     QEIDLSNTTFLLQKQGGEWLVCGQK"
     gene            complement(6371..7255)
                     /locus_tag="PLW25_04250"
     CDS             complement(6371..7255)
                     /locus_tag="PLW25_04250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOS53732.1"
                     /translation="MSFIIWGVPGRQFILHTREFIMRSRLLLILLFVALLTTGAGSAP
                     EAVYSFSQAQNTERSPERWREWPVVPELSEHAVQILASAATNTSLNLRSISKIGDCQF
                     TPATFLAGYVKGDYPLPEEYERTAHYFKESMVRDSITAANGLGVSSVLNPMFAAGAGY
                     QECQRTETPLDCELRTRRPVVVLVALGTNWKPRQEITFEEHLRTVVETILASGALPIL
                     ATKADNIEEDWKLNLANARVAADYDIPLVNVWRAVQPLPNNGLQAPNNVYLTADGWVE
                     QNRAWLRMLDKVRVVLEP"
     gene            7308..8045
                     /locus_tag="PLW25_04255"
     CDS             7308..8045
                     /locus_tag="PLW25_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS53733.1"
                     /translation="MAPKTPFLSIVFPAYNEELRLLETLQRTLAFIHTLPYPAEIVVA
                     ENGSVDGTFEIARSLSVLHPEITTLHLEKAGKGRAVQAGMLAARGRYRFICDVDLSMP
                     IEEVPRFLPPQIEGEDIVIASREAPGAMRYDEPWFRHFIGRIFNNMVRWMVLPKLQDT
                     QCGFKCFSDEAAEALFPLMTIPGWTFDVEVLAIAFKMGFRVKELPIPWYYHPHSKVRV
                     VRDSVRMASDLLKIRRNLRRGEYDLQG"
     gene            8029..8700
                     /locus_tag="PLW25_04260"
     CDS             8029..8700
                     /locus_tag="PLW25_04260"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOS53734.1"
                     /translation="MTFRVDPGLIPAAPDLAFEFALWENGLESVAGIDEAGRGAWAGP
                     VTAAVVVFPAYFALPQNLVFIRDSKQLSAEKRTALEPLIKQKAMAWSVGWASNQEIDC
                     LGILPATRLAMTRALELLKLTPQHLLIDALFIPEYPCGQTALLKGDQRALSIAAASIL
                     AKTARDKFMGEIASKISGYAFERNKGYGTRAHQAALSSHGPCSAHRFSYAPVKRSLKK
                     QQVEG"
     gene            complement(8730..11612)
                     /locus_tag="PLW25_04265"
     CDS             complement(8730..11612)
                     /locus_tag="PLW25_04265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOS53735.1"
                     /translation="MSDIKTPQQKTVDNEDISISWHSLSADEALQRLESFRAEGLTSA
                     EAEHRRKEYGLNQLEEKPRRTFIQMVIEQLRSFVILVLMGAAILSAVLGEYIDAGAIV
                     AIVLLNTILGVVQESRAEEALAALRRMAAPESQVLRDGKRISVPSSHLVPGDIVFMEA
                     GYYVPADVRLLEAVNMRIDEAPLTGESVPVQKNAALTLDPLSSLGDRKNTAFMGTVVS
                     YGRGKGVVTSTGMHTQLGIIARMLQSVQEEQTPLQRKLDQLGKTLGLAALVVCGLVFV
                     VGLVESIGAISADLIVGLFMIAVSLAVAAVPEGLPAVVTVSLALGMQEMIKRHALIRR
                     LSSVETLGSATVICSDKTGTLTQNEMTVTHIWVDGHMVKVTGQGYTPIGSFTIGEREI
                     HLDQYAGVRTALWVGALNNDAVLEETEREDGEKEFRMVGDPTEGSILVAAYKAGGEIA
                     ALNKAYPRTNEIPFDSDFKRMVTVHTVDDPRLNDISPYSEEKGRGSIVVAVKGAPDVV
                     LNLCTHYQPIDNGVQAVLDEDTKQQILAANDEMTGNALRVLGVAFRMVNEMPSQVERE
                     ELEKDLVFIGLIGMIDPARPEVNPALQTAARAGIRTIMITGDYPNTARAIAESIGLLG
                     KGRHVLTGAQLEAMSDDELKEEVRRTDVFARVSPEHKLRIVNALQANGEVVAMTGDGV
                     NDAPAIKQADIGVSMGITGTDVAKDTADMVLTDDNYVSIVSAVEQGRIIYSNIRKFVY
                     FLISCNMAEILIIFLPTALGFLLFPQLTTEQLAPLAPIQLLWLNLVTDGAPALALSTE
                     KGDPDIMDQPPRAPKEPIINRQMLLGVTTQTIAISAATLTAFFTGLNYQGLSPENNLD
                     IARTMAFVTLSSSELLRAFTARSERYPLLKQGIFSNKWMNIAVLFSLLLVLVVVYVPF
                     LNPIFKTVPLAWQQWQLLLPLLVVPSVVAELVKWFSYTHGKKTRLA"
     gene            complement(11634..12890)
                     /gene="glgC"
                     /locus_tag="PLW25_04270"
     CDS             complement(11634..12890)
                     /gene="glgC"
                     /locus_tag="PLW25_04270"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOS53736.1"
                     /translation="MGATDDILAVILGGGRGARLYPLTKERSKPAVPMAGKYRLIDIP
                     ISNCINSGIYHIAVLTQFNSVSLHRHISRTYNFDAFHSGWVQIWAAEQTLENTNWYQG
                     TADAVRKQLLEISSARTSHVLILAGDHLYRMDYAKMAEFHLEKDADITVAVQPVSTVD
                     CQRFGILKRTPNCQITDFHEKPTDPEVLKDIISRDDPARPYLGSMGIYLFKTKVLQEL
                     LENERHDDFGSHVIPTAIRSHRVYGYDFHGFWEDIGTIRSFYQANLALTDLNPPFNFY
                     DQNFPIYSHPRFLPGSNIEDTIMKNVLIAEGCRIEKATLIHSIIGLRSQIRGGTTIID
                     SILMGNDYYEKPGSPEAFAGALEIGFDCYIEGAIVDKNARINKGTIIKPFPVGTDIDG
                     DGWLVRDGIVIIPKSAVIPPGTEITP"
     gene            complement(13028..13687)
                     /locus_tag="PLW25_04275"
     CDS             complement(13028..13687)
                     /locus_tag="PLW25_04275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOS53737.1"
                     /translation="MIPIGDDNSQRRLVPVVTWILVILNVAVFLLELMGGDVFIMMWS
                     FIPARFMQDPAGNMITIFTSMFMHAGWFHLIGNMLYLIIFGDNVEDRFGHVKFLLFYV
                     LCGFIAMLAQLIAAPYVTIPNLGASGAIAGVLGAYLVLFPRRRVRVLLGWWIVHLPAL
                     IVIGGWILIQVLSSLGSLAGSASAAGGVAYMAHIGGFLSGLVLSFFFRNRVRALPPSY
                     M"
     gene            complement(13904..16429)
                     /locus_tag="PLW25_04280"
     CDS             complement(13904..16429)
                     /locus_tag="PLW25_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOS53738.1"
                     /translation="MERFTQRARRVLSLAHQEAERMRHTAINTEHILLGLIEEEGGIA
                     GHALRELGLETARVQEMVERLAPIGLQESSALELSVGAQQALEFSIEEARKLGHQYIG
                     TEHLLLGLTRVTDGLALEVLKKLGVSAEQVRRQTRRVMEESASGPASAGGAPLARREE
                     KKEAKTPLVDQLAVDLTTLAEEGKLDPVIGRQQEIERVIQILARRNKNNPALIGEPGV
                     GKTAIVEGLAQRIIEGDVPAPLIGKRVLQLDVGSLVAGTMYRGQFEERLKRVIDELKA
                     SRSILFIDELHMLVGAGSAGSAVDAANILKPALSRGELQVVGATTLDEYRKHIETDAA
                     LERRFQPILVVEPTVEETIQILKGIRKAYEEHHRLVISDEALEAAAHLSSRYVTERFL
                     PDKAIDLIDEASSRVRMYKSPAAQTFKELMLELKDVKAHHSEAVQSGRSDDAMELSNK
                     EADLRGRIEKLRTGWDRTTSPVVNAEDIAELVSMWTGVPVVQLATEESERLLRMEEEL
                     QKTIIGQEEAIKTISKAVRRGRAGLKDPRRPIGSFMFLGPTGVGKTALTRALALFLFG
                     SEDALIQIDMSEFMERHNVSRLVGAPPGYVGYDEAGQLTESIRRHPYSIVVFDEMEKA
                     HPETHNMLLQIMEEGQLSDARGRKVDFRNAIIVMTSNIGSDLIRRQGSLGFTLQRDEK
                     ENEAKNYEDMRKKLLDSLRKVFRPEFINRLDGVIVFHPLSREHIRSIVHLELDKVAER
                     MKEHNITLRASAAALEHLSAEGYNPEMGARPLRRVIQDSVEDRLSDAVLSHEFNAGDA
                     VIVDVTKEGEIILKRNRKRAVALKPGKSAEGETQAEEPERVQV"
     gene            complement(16698..17582)
                     /locus_tag="PLW25_04285"
     CDS             complement(16698..17582)
                     /locus_tag="PLW25_04285"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOS53739.1"
                     /translation="MVATLEGQRQNAPRALAAHVDTLGAMVKNIKPSGRLSLSKIGGF
                     AWNTVEGEGCTVFTRSGRRVRGSVLVTAASSHVYGAKTAELKRDDDTMEVRLDEKAAS
                     VEEVKSLGIDVGDYIAFDPRVELTNGFVRSRHLDDKAAVACLVEALRALRSEGLKPAQ
                     TTYFFISNFEEVGHGAANGLPAEAGELVVVDMAAVGDGQNSDEFHATLCVKDSSGPYH
                     HGLSNHLRKLADNHGIPYKVDIYPYYGSDGSAFWRAGGEAAVALIGPGVDASHNYERT
                     HTDALTATANWILAYLLD"
     gene            complement(17756..18178)
                     /locus_tag="PLW25_04290"
     CDS             complement(17756..18178)
                     /locus_tag="PLW25_04290"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOS53740.1"
                     /translation="MNPFKYHYPIDVRFGDLDAFWHVNNARFLVYLEDARSRYLQEMG
                     LLDGKALWQLPIIVGDIHIRYRQPIELGHKVVVSMGATRISAKTVTFEYQITGEGGTP
                     LHATAESIMVCYDYETKKTVPLSDELRRKFSEREGREL"
     gene            18333..18734
                     /locus_tag="PLW25_04295"
     CDS             18333..18734
                     /locus_tag="PLW25_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOS53741.1"
                     /translation="MKITRQADYALRAMVYLSQKSDSPTSTKEIAKAQGIPPSFLAKI
                     ISQLSLKKLIIASRGANGGVALARPASNISLLEVIEAIDGEIALNQCTVSIGNCDRAE
                     DCPLHIIWQETQDELVTKLRNTTFDRFSKKN"
     gene            18772..19029
                     /locus_tag="PLW25_04300"
     CDS             18772..19029
                     /locus_tag="PLW25_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53742.1"
                     /translation="MATGTPINRQMVIVLKNGIIAIDWGDGLYQDIRTGDFIPVLETD
                     YSHHILNEELDWLIKIGRVISYDKNTVQSQSLPERPQRTIE"
     gene            19096..>19472
                     /locus_tag="PLW25_04305"
     CDS             19096..>19472
                     /locus_tag="PLW25_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS53743.1"
                     /translation="MLTHKTLLQRIAFFTGLRLTLNTSIRMIYPYLAVFAVALQRDIG
                     AISLVLAASMFTSAVGPFIAPIADRRGRKVGMLIGMGIFLAGTFSASLFPSYFTFFLA
                     ILVGNLGNNIFLPSMQAYLGDLVP"
BASE COUNT         4433 a         5455 c         5137 g         4447 t
ORIGIN      
        1 ttttttgaag ggataaggag gatagaaatg ccaagcatta tcgatattga agggattggc
       61 tccgtttatc aaaagaaatt aaaagccatt ggcgttgcca ctactgataa attactggaa
      121 ctcggcgcta cccccaaagg acgcaaggaa ctggccgaga agactggcac cggggaagcg
      181 ctcatccttg aatgggtgaa ccattccgac ctgtaccgca tcaaaggtgt gggcagtgag
      241 tattctgacc tgctggaaga agccggtgtg gataccgtag tcgagttgag caagcgtgtg
      301 gcgaaaaacc tctatgaaaa aatggtggaa gtcaacgctg ccaagaaact cgtccgaaaa
      361 ttgcccgttg agaaacaggt ggcagactgg atcgaacagg ccaagaaatt accacgtaag
      421 gtgagctatt aaaaaccgtc cttcagcaca gggtgagaga gtctctcacc ctgttttcgt
      481 attgaacaat ctcggcaaag cgctcttttt ttatgaaact ggtttatctg gcatctgaaa
      541 acccggtaaa aatcgctgcg gttgaggagg gtttcaggcg cgtgttcacc ggcgaggagt
      601 tcagcgtgcg ctcggtgagc ctttcgctca acctgcccgc gcaacctaaa accgatgatg
      661 aaacgctctt ctgcgctgaa cagcgggcag cgaacgcgcg cgagacggcg cctgacggag
      721 attattgggt gggtattgag ggggggacca gcgaacgcgg cggggaaatg ggcaccttcg
      781 cctgggtggt cattcttgat tcgttacgca gggggatcgg ccgcagcggt gaattttttc
      841 ttccaaaaat actggcggac ctggtacggc agggtatgga gctcggagac gccgatgacc
      901 tggtcttcgc gcggcaaaat tccaagcaaa aaaatggcgc ggtcggtctg ctgaccgctg
      961 acgccatcga caggttggag ctctatattc ccgcggttat cttcgccctt atccccttca
     1021 aaaaccccct gttatttccc tgatcgttct ctttttgcgc caatacctgt ctgtcatttt
     1081 tctgattgca ggcgggtgaa tgcttgtgcg taccagcgca ggtaatccgc cacgtgcgcg
     1141 ctccagtaag aaggttcatg cctgccttca aactcgaaga actgataggg taccccggca
     1201 tccttcagcg cggtgacaaa ttcctgtact ttttcttttt cagcatcctt atcacccgca
     1261 tccaccagca ccaaggggat actttccggc ggcaaaccag ccgccgcctg aatattccat
     1321 aactcaccgt aaaaaagagg tgtgctatga gcgcccacga taccgaacac ttcgggggat
     1381 acaaaaccca ggtgcaccgc ccaattgccg ccgcgcgaaa gcccgccaat cgcgcggcag
     1441 gtacgctctt tgcaaaccgc gtaattttcc tcgatataag gcagcacgtc ctccatcaac
     1501 gcttcgtcaa attgagattc ccgcggtccg ggcgtccagg tgacctcaaa gggcatcacg
     1561 atcaccaggg gttggatctc tttgctcgtg atcaaatcgt ccgccgcgtt ggtgagtccc
     1621 agctccagcc attgtgcgtt ggtggctgcc tgcccgtgga gcataatcaa ccaggggtag
     1681 ccgccctcct ttttggggtc gtaacacgga ggcaggtaaa tattggtgtt tatcgggcgg
     1741 ttgagctttt gcgtgggaat atccacgttc agaatgctgc cctgtgtggc tgtgcagaca
     1801 aggggcgtgg gtgtgcgggt tgcctgcgga acaggcgtgg acgtaggatg aggggttgaa
     1861 gtgtcagtag cggctactgt tggaattggg ctgctgttca ctgccgcgca actgaccaga
     1921 aaaagacagg tgagcaggct gatgagtgtg gtggcggtgc ggggcattgg tttttcgacg
     1981 tgctcctcgg ggctcagttt gcttgacgtt atccagcaat ccggattata ctctatgcgc
     2041 tcggggtgta gcgcagttgg cagcgcaccg cgtttgggac gcgggggtcg gcggttcaag
     2101 tccgcccacc ccgaccatca aaccagaaaa tctccatacc gctggcagcg gtgaacctgt
     2161 tcgttcttct tattcctgaa ccataaccaa accagtctgc ccgtttttta cctccttgcc
     2221 attcgctgga tgggcgtgtt ttgaaggatg gaagtaacaa tgtatcagcg atgcgggttt
     2281 tacgggggac agtgcctttt cctattgaat caagccggcg tctatgagcc tgcgcgccag
     2341 catttcttct ccttgagcat caaggtggat ggcgctattg gtgaagtgtt cctgcggcac
     2401 caggtcccac aggtcgaaat atttccagcc gttggctgag ctggcttccg ccagggtctg
     2461 gcggtacagg tcataagccc agcgcggata atagaagtta tagcggatat cactgttctc
     2521 gccgttgctg actaggattg gttcgttgac cagcacgacc ggcacctcgc cggcgatttt
     2581 catccccgcc tgtaacaggt ccagcgccag tccgtcttca gggaaggcgg gcggctgcca
     2641 gtggtggaag ctctcatctg cttccaggtc acgcccggcg ggcgggtaag agggcgggta
     2701 aagctgatcg atccctgtgg cggtccacat cactccgtag agctgcaagc gcagcaaatc
     2761 tgccagttca cggcgacgcc cgatgagagt acgctgccag aatgttggct ctcctgcagg
     2821 catggtgata gccaactggt aacgctcaat gagttggcgc acccgttgcg ggttatgggc
     2881 cacgatgggc gattccagct ggcggtcgcg cgggaaggat tcaagagtga cccgccacag
     2941 gatgaggtct ggctggtaac tcatggcccg ctcaagcacc atcaggtctt tcaccaagga
     3001 gagggtgggg tagcccaggt tgtacacccg cacccgtctg ccatcagcgc ttatcaaccc
     3061 agcggcgtct aattgacctg ccagtgtctc ttctggtttt aagagcgtgc cccaggtagc
     3121 gctgtctccg atcaccagca cgcgaaattc atcagccgct ttgggggtac catcgagcct
     3181 gtgggcggcg aacatggctt caaagttgta caggctgaag ttgtatgcct ctctgggcgt
     3241 ctcaccgaaa ggaaagcgtt ctcgtccgcg caacagggaa ttgtaagccg agattctgcc
     3301 cagtccgtcg gatggtatca gggcgtaggc taaatggata agcgcgaaaa gcagcagccc
     3361 cttgagcagt acattgcgga taaagcgggg tgtcacagcc cgcctccaaa cccgaagagc
     3421 ctgaccagca cgcgcgtaga taaaccgggc tcaggcaggg taaaccacac ccaccccagg
     3481 gtgacataaa taaaggtcag cagcgtgttg agaacatgaa tggcgcggga aagcccggtt
     3541 ttggcctgcc agcgagcggc caggtgctgc gttcccgcgc tccagcgatt ctgcacgaac
     3601 aagcccaacc cgtgccaggc tccccacagc aaaaaattca tcgtgatgcc atgccacaaa
     3661 ccaatcagca ccatggtgct caattgcgtc accaatatca ccagccatgc cggcatccac
     3721 ctggcgcggt tgcgtaaaaa acgcgtgacc ggaaagaaga agtaagcccg catccactgg
     3781 gtgagggtga tatgccagtt gtcccaaaag cgcgtgaggt tgctcttcag gtaaggcgcg
     3841 cggaagtttt caggcaggcg gatgcccatg aaacgcgcca tgcccagggc aatatccgtg
     3901 tatccgctga agtccaggta aatttgcagg ctgtaggcca gcagcatcaa ccacaaccaa
     3961 cccgcggcgt ctacctgggc cgcattgctg gcgttgagag agacgataga cagggtatcc
     4021 gccagcacaa acttcttgaa cagacccagc atcaggcgtg tgctgcccgc accaaaatcc
     4081 aacgcgcctg ggataaattc accgcgcagg tctttaatga aacgctcgct gcggtcaatg
     4141 ggtccggcgc tgatagcggg gaagaagagg atgtagatca ggtactcgcg tagcgccatt
     4201 tgcggcagcc tgccagcctg ccggtcgcgc agcgtgtgga tcagccggaa agcgatgtaa
     4261 gaaaatccca gccagcgcac atccagcgcg gatgcaagct cgcgggattg accgctgaaa
     4321 ccgcgcaaca gtgcgcttgt ggtcaacgtc agcgcaggca gtttgagcac caggaaaagc
     4381 agcaaaacca ggatgatcgc cagcgtcaat gcgggtttga agcggtggct gatgcgcgcc
     4441 gccatcaaca acaaccccag gttcaccagc acaacaatcc ccacggtcaa cgtctggggc
     4501 ggacgtgaag cgctgagcaa cccgccgctg cctaaataac gtgtgagggc aatgaccaga
     4561 acggtcgccg taacgatgcc cagcgtcacc aggatttcac ggcgctggcg ttcttctggt
     4621 aatgaggtca gtgcccagcc caatccggcc agtgagaggg aggcacacgg cagccagaaa
     4681 tccaacccgc ggataggcag gatgggttgc agccagaaga tgatcaccac cgagctgacc
     4741 agcagcaaca caacgcgcca acgccgtgcg cgccggatgc tgcaaaccac cagcacacca
     4801 agagagatac caatcagcac caggatacgg agcagcgtca tttaaaaacc ctggtagatc
     4861 cacagcggcg cttcgccaga agagagaatg accagccaca ccaatatcag cgcgaaagcc
     4921 agcgcccaca ggttttcttt tgagaaaata cgtttcatgc tatgttctcc actgaaaaat
     4981 catcctggtg tttactgatc aaccgccgag aaagcgcagc ggaaacccag gtcatcatcc
     5041 gcgtaatacg gactgaccca atagcgttcc gcgctgcgaa taagaaaggg gctgaacagg
     5101 taagagccac cgcgcgcaat acggtgcgcg ccattttctg gcccgcttgg gtcgtaggtt
     5161 ttataaggag aggtagtgta ctctccatac cagtccagta cccattccat cacgttccca
     5221 gccatatcca gcgcgccata cgggctggcg cctgccggat aactgcccgt ttcagttgta
     5281 tctccgacca gcaggtcata gttggcgtgt agcgtcccgg gttctatgtt cccccatggg
     5341 tagatgaaac cttcgcttcc gcgggctgcc ttttcccatt ccgcctcgct gggtagtctt
     5401 ttgcccgccc accggcagta acgaccggcg tcgaaccagt tcacatacac aaccgggtaa
     5461 tccaggtact gcgggttgcc ataatactct tctcgtgtga aagaactgga gcgtcggggt
     5521 tcattacaga cgccgtctgc cacacaggcc ctgtacatgc gattggtgac ctcggtttgg
     5581 tctatccaga aggcgttaag ttgaactgta tgctgcggat actcatcctt ttcgccttcc
     5641 cccttcagtg agcccattaa aaaatcacct ttggggataa aaagcatgat cgcgccatcg
     5701 tccaatgcca tctgcgtacg ggcaggggtg gggattggcg tgtcaggctg gtgggtcgct
     5761 ggcgatagga tggttgggtt gctggttggc gctgcctgcg agctcgaacg gacctcgtcc
     5821 tccacgaaag gcgagaaggt tgccacacgc tccgtcgccg tgggcgcttc agcgcagcca
     5881 caaagcgcca gcgtgataat gaccagcagc agacccctga aagcgctggt ttcgcttact
     5941 tttgcccgca caccagccac tcgccgccct gtttttgcag cagaaacgtt gtgttggaaa
     6001 ggtcaatctc ctggtcttct gtgccataag tggcaatgat cttccccttg cagttgacag
     6061 cggcgctact accatcgttc tccaattctg aacagacgat gttgtctacc cttgccttga
     6121 ccccggtaaa cgaatccagc attaacttgg attgctgctc ccaatccgcg caagaaagct
     6181 ggctcaatga agacgcgtct ccggcaacca gtgcgttata gtacgccact accacatcag
     6241 ccggcgaatt ggagccggaa caggatacca gaaacaaaca aaacccaagc agtagaagta
     6301 ctgttttcca tcttttcatc atcatatcct cgtcaaatta agattatttc cagtttggtg
     6361 ggtgccgcgg tcagggttcc aacaccacac gcaccttatc cagcatcctt aaccaggcgc
     6421 ggttttgttc cacccagccg tcagcagtga ggtacacgtt gttgggcgcc tgcaagccat
     6481 tattgggcag tggttgaacc gccctccata cgttcaccag cgggatatca tagtcggcgg
     6541 ctaccctggc gttcgccaag ttgagtttcc aatcctcttc aatattatcc gctttggtgg
     6601 ccaggatagg cagggctccg ctggcaagga tggtttccac cactgtgcga aggtgttcct
     6661 caaaggtgat ctcctgcctg ggtttccagt tcgtgcccag ggctaccagc accaccaccg
     6721 gtcggcgcgt gcgcaactca caatccaagg gtgtttcagt tcgctggcat tcctgatatc
     6781 ccgcaccagc ggcaaacatc gggttcaaca cactgctgac ccccaatcca tttgcagcgg
     6841 tgatcgaatc cctgaccatg ctttctttga aataatgagc ggtgcgttca tactcttcgg
     6901 ggagagggta atctcccttc acataacctg ccaggaaggt cgccggggtg aactggcagt
     6961 cacctatttt ggaaatcgaa cggaggttaa gcgaggtgtt cgtggcggcg gatgcgagga
     7021 tttgaacagc gtgctcagat agttccggca cgaccggcca ctcccgccag cgctcaggtg
     7081 aacgctctgt gttctgcgcc tgagaaaaag aatacaccgc ttctggagcg cttcccgcgc
     7141 ctgttgttag caaggctaca aatagaagaa tcaacaacaa ccggcttcgc atgatgaatt
     7201 ctcgcgtgtg gaggatgaac tgcctgcctg gcacacccca aattataaag gacaaccggt
     7261 ttcttcacac ccgcgaaaag acggctaaaa gcgtataata acaacaaatg gcacccaaaa
     7321 cacctttcct ctccattgtt tttcctgcgt acaatgaaga actgcgcctg ttggaaacgc
     7381 tgcagcgcac gctggcgttt attcacacac taccctatcc cgctgagatc gttgttgccg
     7441 aaaacggcag tgtggatggc acgtttgaga tcgcccgctc gctttccgtt ttacatcctg
     7501 aaatcaccac cctgcacctt gaaaaagccg gtaaagggcg cgcggtgcaa gctggcatgc
     7561 tggcagccag gggacgttac cgcttcatct gtgatgtcga cctatccatg ccgattgaag
     7621 aggtgccgcg ttttctcccg ccacagatcg aaggtgagga tatcgtcatc gcctcgcgtg
     7681 aagccccggg agccatgcgc tatgatgagc cgtggttccg gcatttcatc ggccgcatct
     7741 tcaacaacat ggtgcgctgg atggtgctgc ccaagctgca ggatacccag tgcggcttta
     7801 aatgcttcag cgacgaagcg gcagaagcgc tcttcccgct gatgaccata ccgggatgga
     7861 cttttgatgt tgaggtgctg gcaatcgcct ttaaaatggg tttccgcgtt aaagaacttc
     7921 caattccctg gtactaccat ccgcacagca aagttcgcgt ggtgcgtgat tcagtgcgca
     7981 tggcaagcga cctgctcaag attcgtagaa atttacgccg cggcgagtat gaccttcagg
     8041 gttgacccgg ggctcatccc cgccgcgcct gacctggcgt ttgaattcgc cctttgggaa
     8101 aatgggctgg aatctgtagc cgggatcgat gaagcagggc gcggcgcctg ggctgggccg
     8161 gtgacagctg ccgtggtggt ctttcccgct tattttgccc tgccgcaaaa cctggttttc
     8221 attcgcgact ctaaacaatt gagcgcagaa aaaagaacag ccctcgaacc cctcattaaa
     8281 caaaaggcga tggcctggtc agtcggttgg gcttcgaacc aagagatcga ttgtttgggc
     8341 atccttccag ccactcgcct ggcaatgacc cgcgcgttgg aactgctcaa gttaacccct
     8401 caacacctgt tgatcgacgc gttgtttatt ccggaatacc cctgtggtca gactgctctc
     8461 ctgaaaggag accagcgtgc cctgagcatc gcggctgcct ccatccttgc gaaaaccgct
     8521 cgagataaat ttatgggaga gatcgccagc aagatttcgg gctacgcgtt cgaacgcaac
     8581 aagggatacg gcacccgggc gcatcaagcg gcgctttcca gccatggtcc ctgctcagcg
     8641 catcggttca gttatgcccc ggtgaaaaga agcctgaaaa aacaacaggt ggagggttga
     8701 cctccacctt gcttatcttc tcatcacaat caagccaggc gggtcttctt tccgtgcgtg
     8761 tacgaaaacc atttgaccag ttccgctaca accgagggca caaccagcaa tggtagaagc
     8821 agttgccatt gctgccaggc aagggggact gttttaaaaa tggggttcag gaagggaaca
     8881 tagacgacta ccagcaccag gagcaatgaa aacagcaccg cgatattcat ccatttgttg
     8941 gaaaaaatac cctgcttcag caacgggtaa cgctctgaac gggcggtgaa ggcccgcaac
     9001 aactcagaag acgaaagcgt tacaaaggcc atggtccggg caatatcaag attgttctct
     9061 ggcgagagcc cttgatagtt gaggcctgta aagaaagcgg ttagggtcgc ggctgaaata
     9121 gcgatagtct gggttgttac gccaagaagc atttgccggt tgatgatggg ctctttgggc
     9181 gccctgggag gttgatccat gatgtccgga tcacctttct ctgtacttaa agccagcgct
     9241 ggtgcgccat cggtaaccag gttcagccag agcaactgaa tgggcgccag cggcgcaagc
     9301 tgctcagtgg tcagttgagg gaacaacaga aagcccaacg ccgtaggtaa aaagataata
     9361 aggatttccg ccatgttgca ggaaataaga aaatacacaa atttacggat gttggagtaa
     9421 atgattcgac cttgctctac cgccgaaacg atactgacgt aattatcatc ggtgagcacc
     9481 atatccgcgg tgtctttcgc cacatccgtg cctgtaatgc ccatggaaac accaatatct
     9541 gcctgcttga tagcgggtgc gtcgttcacc ccgtctccag tcatcgccac gacctcgccg
     9601 ttcgcctgca gcgcattgac aatgcgcagt ttatgctcag gtgagacgcg cgcgaacaca
     9661 tccgtcctcc tgacttcttc tttcagttca tcgtcgctca tcgcttcaag ctgcgcgccg
     9721 gtgagcacat ggcgcccttt ccccagcaag ccaatgcttt cagcaatcgc gcgcgccgtg
     9781 ttgggatagt ctcccgtaat catgatggta cggatgccgg cgcgcgcggc agtttgcagg
     9841 gcagggttca cttctggcct ggcagggtcg atcatgccga taagcccaat aaacaccagg
     9901 tctttttcta attcttcccg ctcaacctga ctgggcattt cgtttaccat gcgaaaggcg
     9961 acgccaagca cgcgcagcgc atttcccgtc atctcatcat tggccgccag aatctgctgt
    10021 ttggtgtcct catccaaaac cgcctgaaca ccattatcga tgggttgata atgcgtgcac
    10081 aggttcagca ccacatccgg tgcgcctttg acggcgacca caatgctgcc cctgcccttt
    10141 tcttcgctat acggtgatat gtcgtttagg cgcgggtcat ccactgtgtg aaccgtcacc
    10201 atgcgtttga aatccgagtc aaatggtatt tcattggtcc tgggataggc cttattcaac
    10261 gcggcgatct ccccaccggc cttgtaagcc gccaccagta tcgagccttc agtagggtcg
    10321 ccaaccatgc ggaattcttt ttcaccatct tctctttcgg tttcttcaag cacggcatca
    10381 ttgtttagcg cgcctaccca caaagcggta cgaaccccgg cgtactggtc cagatggatc
    10441 tcgcgctctc cgatggtaaa cgagcctatc ggtgtgtaac cctgcccagt cacttttacc
    10501 atgtgtccat ccacccaaat atgggtaacg gtcatctcgt tctgggtcag cgtgccagtt
    10561 ttatctgagc aaatgactgt ggctgagccc agggtttcga ccgaagacag gcgtcggatg
    10621 agcgcgtggc gtttgatcat ttcctgcata cccagagcca aactcaccgt caccaccgcg
    10681 ggcagtcctt caggcaccgc cgccactgcc aggctgaccg cgatcatgaa gagacccaca
    10741 atcaggtccg cgctgatcgc gccaatgctt tcgacaagcc ctactacaaa aaccagccca
    10801 caaacaacca gggctgccaa gcctagtgtc tttcctaact ggtccagttt tctttgcagg
    10861 ggggtttgtt cctcttgcac agattgcagc atgcgggcga tgattcccag ttgggtgtgc
    10921 atgcctgtgc tcgtcacaac gcctttgccg cgcccataag aaaccactgt gcccatgaag
    10981 gcggtattct ttcgatcgcc taaagaagaa agagggtcaa gcgtcaaagc tgcgttcttt
    11041 tgcacgggca cagattctcc ggtaagcggg gcttcatcga tgcgcatgtt caccgcttcc
    11101 aacagccgca catccgcggg cacgtagtaa cccgcttcca taaaaacgat atcccccgga
    11161 acaaggtgcg aagaggggac gctgatgcgt tttccatccc gcaacacctg gctttcaggc
    11221 gctgccatgc gtcgaagcgc ggccagggct tcctctgccc gtgactcctg gacaacaccc
    11281 aggatggtat taagcagcac gatggcaaca atcgccccgg cgtcaatata ttcacccagc
    11341 acagcagaaa ggatggccgc gcccatgagt acgagaataa cgaaactcct caactgctcg
    11401 atcaccattt gtataaaagt gcgccggggt ttttcttcca actggttcaa gccatactct
    11461 ttacgcctgt gttctgcttc ggcgctggta agcccctccg ccctgaacga ttccaacctc
    11521 tgcagggctt catccgcact caaagagtgc caactgatgg aaatatcttc attgtcgacg
    11581 gttttctgtt gtggggtttt aatatcgctc attggttctt ctttagcgga aaattacggt
    11641 gtgatttccg ttccgggcgg tataacagcc gatttgggga tgatcacaat cccatccctc
    11701 accagccagc cgtcaccatc gatatctgtg ccgacaggga agggtttgat gatcgtgcct
    11761 ttgttgatgc gcgcgttctt atcaacgatg gcgccttcaa tgtaacaatc aaacccaatt
    11821 tccagggcgc ccgcgaatgc ctcgggtgaa cccggttttt cgtagtagtc attgcccatc
    11881 aagatgctat ctattatagt tgttccgccg cgaatctggc tgcgtaaacc gatgatggaa
    11941 tggataagtg tggccttttc gatccggcaa ccttcggcaa tcagcacgtt cttcataatg
    12001 gtgtcttcga tattcgaccc gggcaaaaaa cgcgggtggc tgtatatggg aaaattctgg
    12061 tcgtaaaaat tgaatggcgg gttgagatca gtaagcgcca ggttggcctg gtagaaagaa
    12121 cgaatcgttc caatatcttc ccagaaacca tgaaaatcat acccgtacac acggtgagac
    12181 ctgatggctg ttggaataac gtgagaacca aagtcgtcat gacgctcatt ttccagcagt
    12241 tcttgcagaa ctttcgtctt gaacaaatag atacccatgg agcccaggta tgggcgcgct
    12301 gggtcatcgc ggctgatgat gtctttcaaa acctctgggt cggttggttt ttcgtgaaaa
    12361 tcagtgatct ggcagttggg cgtacgtttg agaatgccaa accgctgaca gtcgaccgtg
    12421 gagactggtt gcaccgcaac ggtgatatcg gcatctttct caagatggaa ctcggccatt
    12481 ttggcatagt ccatgcggta caggtggtct cccgcgagga tgagcacgtg cgaggtgcgc
    12541 gcggaactga tttcgagcag ctgcttgcgc accgcgtctg ccgtgccctg gtaccagttg
    12601 gtattttcca gggtctgttc cgccgcccat atttgcaccc agccagagtg gaaggcatcg
    12661 aagttataag tgcgagaaat atggcggtgc agcgagaccg agttgaactg ggtgaggacg
    12721 gcgatgtggt atatgcccga attgatgcag ttactgatag ggatatcaat gagacgatac
    12781 ttacccgcca tcggcaccgc gggctttgaa cgttctttgg tcaagggata caggcgggca
    12841 ccccgtcccc cgcccaggat caccgcgagt atgtcatccg tcgctcccat tagaaaccct
    12901 cctggttaaa aagaattacc tgaaagacac gtgggtaaaa caagcatact ggagaaaatt
    12961 ctgaccgtca atcatcatgc cgggcttatt gaccatcctc cgcattcgtc atatgagtgg
    13021 acaggtttta catatatgag ggcggtaaag cgcgcacacg gttgcgaaag aagaaggaca
    13081 ataccagccc gctaagaaaa ccgccgatgt gcgccatgta agccacccca ccggctgcgc
    13141 tggcagaacc tgccaggctg cctaagctgc tcagcacctg gatcagtatc cagccgccga
    13201 tgacgatgag cgccggcaga tgcacgatcc accaacccaa gagcacacgc acccgccgcc
    13261 tgggaaagag caccagatac gcgcctaata ccccggcaat ggcccctgag gctcccaggt
    13321 ttgggatggt cacataaggc gccgcgatga gctgggcgag catggcaata aagccgcata
    13381 atacgtaaaa caacagaaac ttgacatgac cgaagcgatc ctcaacatta tctccaaaaa
    13441 tgatgaggta aagcatgtta ccgattaaat ggaaccagcc ggcatgcata aacatcgaag
    13501 taaagatggt gatcatattc cccgcggggt cttgcataaa acgcgcgggg atgaacgacc
    13561 acatcatgat gaacacatct ccacccatca actcgagcag gaagacagcc acatttagaa
    13621 tcaccagtat ccaggtgacc accgggacaa gcctgcgctg agaattatcg tcgccaattg
    13681 gaatcatatc tgccctcgag gtgctctttt tttagaaagt atagatgttt tccccccgcc
    13741 gcgcaatggt aagaaacctc gcctatcctg attgttgcgc atcgagcggt gggtggcaag
    13801 cccaaaacgt tatgcgagag ccggatgaaa aacaccagcc caaaaacagc tggtgtttga
    13861 ttaaggaagc acttgaagaa tgcctggggc gatcgtctct cgtttaaacc tgcacacgct
    13921 ccggttcttc cgcttgtgtt tcaccttctg cgcttttacc aggttttagt gcaactgcgc
    13981 gcttgcggtt gcgtttgagg atgatttctc cctccttggt tacatcaacg atcaccgcgt
    14041 caccagcgtt gaactcgtgc gaaagcaccg cgtcggataa tctatcttcc acgctgtcct
    14101 ggataacccg gcgcagcggc cgcgcaccca tctcggggtt ataaccctcc gcagagagat
    14161 gctccagggc tgcggcagaa gccctgaggg tgatattgtg ttctttcata cgttcagcca
    14221 ctttatcaag ttccaggtga acaatgctgc ggatgtgctc acggctgagc gggtggaaaa
    14281 cgataacgcc atccaggcga ttaataaact caggtctgaa gaccttgcgc agcgaatcca
    14341 gcaatttttt acgcatatct tcgtaatttt tggcttcgtt ttctttttca tcccgctgca
    14401 gggtgaagcc cagcgagccc tggcggcgaa tcaggtcaga gccgatattg gaggtcatca
    14461 cgatgatggc gttgcggaag tctaccttgc gtccgcgcgc gtcacttaac tgaccctctt
    14521 ccatgatttg cagcagcata ttgtgcgttt cagggtgcgc tttttccatc tcatcaaaaa
    14581 ccacgatgga ataggggtgg cggcggatag attcagttaa ctgcccggct tcgtcgtagc
    14641 ccacgtatcc tggcggcgcg ccaaccaacc ggctgacgtt gtgacgctcc ataaattcag
    14701 acatgtcaat ctgaatcagc gcgtcttcac tgccaaagag gaacaatgcc agggcgcggg
    14761 tgagcgcggt cttgcccacg cctgtgggac ccaggaacat aaaagatcca atggggcggc
    14821 gcgggtcttt aagcccggcg cgcccgcggc gtactgcctt ggagatggtt ttaatggctt
    14881 cttcctgacc gatgatggtt ttctgtaatt cttcttccat gcgcagcagg cgttcagatt
    14941 cttcggtggc cagttgcacc accggcacgc ctgtccacat cgacaccagt tcagcgatat
    15001 cttccgcgtt caccacgggg ctggtagtgc ggtcccagcc ggtgcgcaat ttttcaatcc
    15061 tgcccctgag gtcagcctcc ttgttagaaa gttccatcgc gtcatcactg cgtccgctct
    15121 gcaccgcctc gctgtgatgc gctttcacat ctttgagttc cagcatcaac tctttgaagg
    15181 tttgcgccgc cggactcttg tacatacgca cacgcgatga agcctcgtca atcaggtcaa
    15241 tggctttatc cggcaggaat cgttcagtga cataacgcga agagagatgc gccgccgcct
    15301 caagcgcttc gtccgagatg accaggcggt gatgctcttc gtaagctttc cgaattcctt
    15361 ttaggatctg gatggtttct tccacggtgg gttcaaccac cagaatgggc tgaaaacgcc
    15421 gttcgagcgc agcgtccgtt tcaatgtgtt tacggtattc atccagcgtg gtggcgccca
    15481 ccacctgcag ctctccgcgt gatagcgccg gtttgagaat gttggcagca tccaccgcgg
    15541 accccgcaga gcccgcgcca accagcatgt gcagttcatc gataaacagg atcgatcggg
    15601 aagccttaag ttcatcgatg acgcgcttga ggcgttcttc aaactgacca cgatacatgg
    15661 tgccagccac cagcgaaccc acatccagct gcagcaccct tttaccgatg agcggtgcag
    15721 gcacatcccc ttcgatgatg cgctgcgcca ggccctccac aatggcggtc ttgcccactc
    15781 cgggttcacc aataagagcg gggttgttct tgttacgcct tgccagaatc tggatgacgc
    15841 gttcaatctc ttgttggcgc ccgataaccg ggtctagctt gccttcttct gccagcgttg
    15901 tcaggtccac cgccaactgg tccaccagcg gcgttttggc ttcttttttc tcttcacgcc
    15961 gcgccaacgg cgctcctcca gctgaggccg gcccactggc gctttcttcc atcacgcgcc
    16021 tggtctgccg gcgcacctgt tccgctgata cgcccagttt tttaagcact tccagcgcca
    16081 gaccatccgt cacacgtgtg agcccgagca gcaaatgctc agtgccgata tactgatgtc
    16141 ctaacttccg cgcttcctca attgaaaatt ccagcgcctg ctgcgctccg accgaaagct
    16201 cgagcgctga agactcttgc agcccaatgg gagcaaggcg ctcgaccatt tcctgcacgc
    16261 gcgcggtctc gagccccagc tcgcgtagcg cgtgcccggc aataccgcct tcctcttcga
    16321 taaggcccaa taatatgtgt tccgtgttga tcgcggtgtg gcgcatgcgc tctgcttcct
    16381 ggtgggccag ggagagcacc cgcctcgcgc gttgtgtgaa ccgctccata cctgccaaaa
    16441 caaatcctcc tgataactct cagccattaa tcaactggaa aactgatcaa tacggcggat
    16501 gttattcgcc ctcattgtac cgttattcct tgtcattgta atcaacaaat ataaatatct
    16561 gataagaatt ttggtttttt acgcacaggt atttatatgc tgcggaaaga ggttacagca
    16621 caggcagaaa tgagcgagtg taagtattta ccggtcagac ctggtcggga atcgatcttt
    16681 atggctgttt cacccgttca atctaacagg tatgccagta tccagttggc tgtcgccgtc
    16741 agcgcatctg tgtgggtacg ctcataatta tgtgaggcat ccactcccgg gccaatcagg
    16801 gcaaccgccg cctctcctcc cgcgcgccag aaggcgctgc catcagaacc ataatagggg
    16861 taaatatcca ctttgtaggg aataccgtga ttatctgcca gtttgcgcag gtggttgctc
    16921 aacccgtgat gataaggacc gcttgaatct tttacacaca gggtcgcgtg aaactcatct
    16981 gaattctgac catcgccaac agccgccata tccaccacca ccagttcacc tgcttcagcc
    17041 ggaagcccgt ttgccgcgcc gtgacccacc tcctcaaaat tgctgatgaa gaaatatgtg
    17101 gtttgcgcag gctttaaccc ctccgaacgc agcgctcgca gggcttccac caggcaggcg
    17161 accgcagcct tgtcatccag atgacgcgag cgcacaaacc cattggtcag ttccacacgt
    17221 gggtcaaagg cgatgtagtc gcccacgtca atccccagtg acttcacttc ctcaacagaa
    17281 gccgctttct catccaggcg cacttccatg gtatcgtcgt cgcgcttcag ttcagcggtt
    17341 ttggcgccat acacatggct cgaagccgcg gtcaccagca ccgaaccacg tacgcgtctg
    17401 ccgctgcggg tgaagactgt gcagccttca ccttccactg tattccaggc aaagccacca
    17461 attttactga gcgaaagcct gccgctgggc ttgatgtttt ttaccatcgc gcccaacgta
    17521 tccacatggg ctgccagggc ccgcggcgcg ttttgtctct ggccttctaa cgtggctacc
    17581 agcgcgcctt tacgtgtgcg gctggttttt actcctggaa tggctttgaa cgcccgttca
    17641 acgagcgccg tagcctcttc agtgtaaccg gttgggctgg gtgtcttcag caggtcaacc
    17701 aggaacttaa gcagtaatgc ttcgtcaatc aggggtaaat cgttttctgc tttctttata
    17761 attccctgcc ttctctctct gagaacttgc gccgcagttc atcgcttaac ggcacggtct
    17821 ttttcgtctc ataatcatag cagaccataa tggactcggc cgtggcgtgc agcggcgttc
    17881 cgccttcgcc ggtgatttga tactcaaagg tcacggtctt ggcgctgatg cgagtggcgc
    17941 ccatggagac caccactttg tggcccagtt caatgggctg ccgatagcga atgtgaatgt
    18001 cgccaacgat aatcggcaat tgccataacg ccttcccatc cagaagaccc atttcctgta
    18061 agtagcggct gcgcgcgtcc tccaggtaga ccagaaaacg cgcgttattg acatgccaga
    18121 aggcatccag gtcaccaaac ctgacatcaa ttgggtaatg gtatttgaat ggattcatga
    18181 gaatattata cttgaggttg tttacccaaa gctcgagcgc cgggaggtca gcgcgcctcg
    18241 atgatggttg gcatcttttc cttcgtcgat tatgtagtaa aatattgaaa gaggacaatt
    18301 ttgacccttt ttacttatag aggtgttttt ttatgaaaat tacccggcaa gccgattatg
    18361 cgctcagagc gatggtatac ctctcgcaga aatccgattc tccgacatcc accaaagaaa
    18421 tcgcgaaagc gcagggcatc cctccttcat ttttggcaaa aattatctcc caactttcgc
    18481 tcaagaagtt aatcatcgca tcgcgcggcg caaatggtgg cgttgccctt gcgcgcccgg
    18541 cttccaacat ttcgcttctt gaagtgatcg aagccattga tggagagatc gcgcttaacc
    18601 aatgcactgt ttcaattggt aattgcgata gagccgaaga ttgccccctg cacatcatct
    18661 ggcaggaaac ccaagatgaa ctggtgacaa aactacgcaa tacaaccttt gatcgcttca
    18721 gtaaaaaaaa ctaacagcag tctaacggta atctgcgaaa ggaaggggca ggtggcgaca
    18781 ggaacaccga tcaatcgaca gatggtcatc gtactgaaga acggcatcat tgctattgat
    18841 tggggtgatg gtctctacca ggacatccgt actggtgact tcatccccgt gcttgaaacc
    18901 gattacagcc atcatatact taacgaagag ctggattggc tcatcaagat tggtagagtc
    18961 atttcatacg ataaaaatac tgtgcaatct caaagcctgc cggaaaggcc gcagcgaacc
    19021 atagaataag ctgattagtt gcgctttttc tcccggccag accgggagtt tttatttttg
    19081 gtggataatt agaccatgtt aacgcacaaa accctgttgc agcgcattgc cttttttact
    19141 ggactgcgcc tcacgctgaa cacatctatc cgcatgatct atccttacct ggcggtgttt
    19201 gcggttgccc tgcaaaggga tattggcgcc atctctcttg tactggcggc ttccatgttc
    19261 accagcgcgg tgggcccttt tatagccccc atcgccgacc ggcgcggtcg aaaagtgggc
    19321 atgctcatcg gcatgggcat cttccttgca ggcacgttct cagccagcct ctttccctcc
    19381 tacttcacct tctttctggc catcttggta ggcaatttag gcaacaatat cttcctgcct
    19441 tctatgcagg cttacctggg cgatcttgta cc
//
LOCUS       DAOSPM010000031        19298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2838_length_19298_cov_13.884581, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000031 DAOSPM010000000
VERSION     DAOSPM010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19298
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2838_length_19298_cov_13.884581"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /gene="nuoK"
                     /locus_tag="PLW25_04310"
     CDS             <1..206
                     /gene="nuoK"
                     /locus_tag="PLW25_04310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOS53744.1"
                     /translation="MLNAANINLLSFWRYLHAPRGDMDGLIFALIVFVVAAAEVAVGL
                     GLIVSAYRRRRTVVANELDLLKG"
     gene            219..2417
                     /gene="nuoL"
                     /locus_tag="PLW25_04315"
     CDS             219..2417
                     /gene="nuoL"
                     /locus_tag="PLW25_04315"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOS53745.1"
                     /translation="MFNIHPLIWLIPLPPLLGFLLIVLFTRRSHRASHSVALAGAGLS
                     FVGSMVVFIKAVTAGGVADSSFAASINWLPTGASWLRIGVLVDPLTAVMLFFVAWTVL
                     MIFIYSIGYHNFGQPIGKQDRPGLPPQGAEITEAGKIHRVPSVEPMYARFFALLSLFA
                     FAMYLLVVSDNLLTLYIGWEIMGFCSYFLIGFWYGKESARKAGIKAFITTRIGDVFML
                     LGMVALYSVTGTLNFHEIFSNPETMTLLTSTLSPVLGLNWAGLIGLLLFCGTIGKSAQ
                     FPLHVWLPDAMEGPTPVSAMIHAATMVSAGVYLSARMFPLMSAGWHHGASLTVPMMAM
                     ALIGGFTALFTSTIALAQNDIKRVLAYSTISQLGYMIAALGIGAFTAAVFHLVTHAFF
                     KALLFLGSGSVIHGMEHGALHTGKHIDAQDMRHMGGLRKKMPVTFWTFLAGGLALSGF
                     PLITAGFWSKDEILSGAFGGGHLALFIVLAVTALLTAFYTARQITLVFLSKPRSDAAA
                     YAHETKPVMTAPLVVLAVFAVTFGWVGIPHAFPGLGAISPAWFQSFVGSMLSPGSVSE
                     AHSNIPLVVSILVSLGGLLLGWLVYRGYESAQEPDPLQKRFGGVFRLLEHKYWIDELY
                     QKVVINPVLWLADRVIYQFIDLRLIDGFLHGLAAAGVWLGNLLRSKFDLPVVNGAGDN
                     LAGGTRGLGDFLRRLQTGRVQQYLAIAVACTVVAALIILSYLAKVQAGSG"
     gene            2414..3949
                     /locus_tag="PLW25_04320"
     CDS             2414..3949
                     /locus_tag="PLW25_04320"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOS53746.1"
                     /translation="MNILSAHLLSLILFLPLVGAVVLLAFPANKVKAIRGVALVASLI
                     PLALTLVLWGSYSTALTVGEFKFVENYPWFTSIGSSYHLGIDGVSLVMLLLTTLLTPL
                     AIMTSFNIKERVKAFMILFLVLETGMLGVFMALDLVLFFLFWEIGLVPMYFLINQWGG
                     EKRNYASLKFILFTMGGSLGLLLAIQMIGVVCGTFDLVTITASWPALQGGELFGIPIA
                     TVKAIAFWAFVVAFAIKVPVWPFHTWLPDAHTEAPTGGSMVLAGVLLKLGGYGFIRLV
                     LPLFPAESARFASWLAALATAAIIFGALAAAAQTDFKRLVAYSSINHMGFVVLGIAAA
                     AWAGGQTNPTLRESAVIALNGAVLQMFNHGLTAAGMFFLVGVLYDRAHTRQLAEYGGL
                     FAILPVYGAILVFISMASLGLPGLNGFVSEFMIVRGVWPIFTLFIALAMIGLLFTAVY
                     VLKALKLTLHGALNEKWVGKLTEISRRELVVIIPLMLLVVIIGWWPAWILNLINQTVM
                     TWF"
     gene            3960..5432
                     /locus_tag="PLW25_04325"
     CDS             3960..5432
                     /locus_tag="PLW25_04325"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOS53747.1"
                     /translation="MPVPLLSILALTPLAAGIIVLLLPAEKRSLIRGIAFATSLFTLA
                     VSVIIFFRFDTTAGGYQFIEKMNWLPALGISYHMGVDGIGAAMELMAGIVVFSGVMVS
                     WRVEDRPREFFAFLLFLAASVFGVFASLDLFQLFFFFELAVFPKYLMIVMWGSPKTRE
                     YGGMKLTLYLFLGSVVALIGVLAVYFTSGLRSFDMLALEQAGLALEFQRAWFLVIFVG
                     FGVLGGLFPFHSWAPDGHVAAPTAVSMLLAGVEMKVGVFAALRAGLMLLPEGMRYWAP
                     LILVLATINVVYGAFIAMIQKDFKYVIGFSSVSHMGLVFIGFATLTREGLTGAGLQMF
                     SHGIMTALFFAVVGMVYDRTHTRDIRELGGLFRTMPIAMLGFVIGGLVSMGMPGFSGF
                     IAEMPIYMGAWQTAPIVAIISALSIVITAAYILLVVRRVFFGETPEGIRAGLSDISLM
                     DKIVVVLLSLILIVIGLFPSVLVPWIEAGVRHILVLTGGA"
     gene            5432..6880
                     /locus_tag="PLW25_04330"
     CDS             5432..6880
                     /locus_tag="PLW25_04330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOS53748.1"
                     /translation="MPAITAEMLLSLLPELILALLALFLLALSLFKKENLGGAYSWVT
                     AFGFGLTLFLTVFYSRPPAEVQLLWGEMIRFDRVGFIFRLLILAGGAMTAIFAGMEQT
                     IAKKAEFYLLLVLSSIGFSLMVSAGNLIMLYLAIETATIPLYVLAGFKQRDEKSVESG
                     IKYFLFGGVSSAILLYGFTLLYGFAGTTRLDGMLELIKQNGAPIAGITLASLLVLAGF
                     AFKMSAAPFHFWAPDVYEGSPTPVAGFLSTVSKAVGFGAFIRVLVNAFGVGSSNAWMV
                     IVAILAAASMFLGNLLAISQRDIKRLIAYSSIAQAGYMLVGVAAGTEFGFSGTVYYLL
                     VYLVTNLAVFAIISWVEKGSGSSAVSAFAGLNRRSPGMALVMMVALLSLGGIPPFGGF
                     FAKVLVFGAAVQSHYVWLAVLGILNSVIALYYYLKIMKVMYLDKPDETSWKVTPALQW
                     RVALALCIACIILLGVIYAPWLNGISLAVTGF"
     gene            6946..7242
                     /locus_tag="PLW25_04335"
     CDS             6946..7242
                     /locus_tag="PLW25_04335"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOS53749.1"
                     /translation="MFDTERDNETRKTNRRNLWLAALAGQVGFLTLIIILAAVVGGLA
                     LDARLGTRPWFTIGLLLASIPVSLLLMVYITRKTVAKIKANHNDHLNEEGRLGK"
     gene            7232..8197
                     /gene="atpB"
                     /locus_tag="PLW25_04340"
     CDS             7232..8197
                     /gene="atpB"
                     /locus_tag="PLW25_04340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOS53750.1"
                     /translation="MENKRKWRWGKHRWILLLLIILSVIGVNLIAPVQPHIQVAAENL
                     SEKPLFTLPVVGDFYLTNTLTAMLLMDIVIILVALGVRRAAKKNELAPGGVGSVFEML
                     VEMLYNLTENTAGKYAAKIFPWFATILIVVLFANLLKILPGFETIGFLHPSEHGFATQ
                     ALGGGWFTLKNTPAADGYVLAPFLRGVSTDLNFTTALALIAVFMIQVIGVQANGARYF
                     EKFINVRTIFKKPLFGVIDFIVGLLELISEFAKILSFTFRLFGNMFAGMVLLAVVGVM
                     LPVFMPSFIMLFELFVGVIQAFVFGMLTMVFMAQATITHNAEAEH"
     gene            8250..8486
                     /gene="atpE"
                     /locus_tag="PLW25_04345"
     CDS             8250..8486
                     /gene="atpE"
                     /locus_tag="PLW25_04345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOS53751.1"
                     /translation="MNGDIVEAAKMIGAGIAMIGALGGGIGVGLSVQGAVQGMARNPD
                     TYGNLLSSMILGIAFSEAIAIYCLVIAFLMLFVV"
     gene            8514..9251
                     /gene="atpF"
                     /locus_tag="PLW25_04350"
     CDS             8514..9251
                     /gene="atpF"
                     /locus_tag="PLW25_04350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOS53752.1"
                     /translation="MEKLGLNLGFLIVQIINFLVILVVLRAWVYTPLQNSLKKRRETI
                     EKGLEDARVAAEARENAERDAAGILAEAQNNAAQLIREAKEKAELAGREVKASVEADI
                     AKTRREAAVEVERERERVLGEIRTNVAALAIAAAQKLVGEALDEKRQHELVAEFFSGI
                     KGGKVTILEENELKGKNAEVVSALPLTRDEQKVIQGEMLSKLGETAAVSFNVDPKLLG
                     GLVVRVGDTVIDGSVAGQIQSLRATLE"
     gene            9328..10878
                     /locus_tag="PLW25_04355"
     CDS             9328..10878
                     /locus_tag="PLW25_04355"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOS53753.1"
                     /translation="MKPVLLSDLFKEFPIPWATDVAPGAIPISAIYYDSRMVRRGALF
                     VAISGGSVDGHEYISSAISRGAVAVVGTREIRGLPVPYIRVADSREALAYLAAAFFDH
                     PARQLTVIGVTGTDGKTTTANMIYEILKQAGVKAGLISTVNAVIGDEVVDTGFHVTTP
                     EAVEVQGYLSRMLAAGLTTVVLETTSHGLAQHRVTACEYDIAVVTNITHEHLDYHGTY
                     EEYRAAKGRMFASLAQTVEKPLGNPRLAILNRDDISYDYLAGLVKGRQVTYGLQHKTD
                     YFAQDVTSNPSGVDFTLIAEGLVERVHCNLPGKFNVSNCLAAFAAARAAGVETGAAIK
                     GLSRLQGVPGRMEAIEMGQPYSAIVDFAHTPNALKVALETAREMTTKRVIAVFGSAGL
                     RDRQKRRLMAEVSVQLADISIFTAEDPRTESLESILAEMADAATLAGGVEGKTFYRVA
                     DRGTAIRLGAELTAPGDLLIACGKGHEQSMCFGTIEYPWDDRTAMRATLAEQLGLPGY
                     EMPHLPTS"
     gene            complement(10943..13186)
                     /locus_tag="PLW25_04360"
     CDS             complement(10943..13186)
                     /locus_tag="PLW25_04360"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOS53754.1"
                     /translation="MKPESKKFITTVGGRPLTFETGKLAAQAGGAVTVQFGDSMVFAA
                     ATMGNEPRVGQDFFPLTVEYEERMYAGGRIPGSFFRREGRPGTDAILVARLTDRPLRP
                     LFNNAIRNEVQVIMFSLSADWENPLDVLAINAASCAVMISDIPWDGPVGAVRVGRVNG
                     EFVLNPTYSQMEESDLDLRIAGTADAILMVESGSNEIDEGSMIAALEFGHAAIQPIVE
                     TQLQMAREIGKEKRAVALYSLDETLVEKVYNRIAEEINTLLDAPHSKHELQDEIDNLR
                     ENIVLEYTDPENTEEVNAYKEAFEETYKRVVRARILEQNKRPDGRKLNEIRPIWCEVA
                     VSPRAHGSGLFTRGETQVLTMATLGTPKEAQEIDNLSPTDSKRYMHHYNFPPYSTGEV
                     KMLRGQSRREVGHGALAERALVPVIPDEKDFPYTLRLVSEVLASNGSSSMASVCGSTL
                     ALMDTGVPIKAPVAGVAMGLIKEGDRHVVLTDIQGAEDHLGDMDFKVAGTTKGITALQ
                     MDIKIKGITPEIMRQALTQAHEARLAILDKMLEVIPVPRPELRSFVPRITTLHIPVDK
                     IGAIIGPGGKIIRALQEETNTKIDIDDDGTVYIAATDGASEATVRERIEALTESAQVG
                     HIYTGKVVRVADFGAFVQILPGIDGMVHISQLDSERVERVEDVVKLGDELTVMVTAID
                     ENGKIRLSRQAVLEGWTPEEALEHDKGGKPGGNRPRSGGDRRGGSSDRRNNRGFSDRN
                     GNRNPRH"
     gene            complement(13350..13622)
                     /gene="rpsO"
                     /locus_tag="PLW25_04365"
     CDS             complement(13350..13622)
                     /gene="rpsO"
                     /locus_tag="PLW25_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOS53755.1"
                     /translation="MALTKEEKQKLIEEYHLHDTDTGSADVQIAILTIRIRQLTEHLR
                     ANKQDNSSRRGLLKLVGQRRRLLAYLRRTDFARFATITEKLKIRTR"
     gene            complement(13760..14569)
                     /locus_tag="PLW25_04370"
     CDS             complement(13760..14569)
                     /locus_tag="PLW25_04370"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOS53756.1"
                     /translation="MPAVDLSRLRARFKLLQLQIEDPVEFTRGLREIFIFHADLTHPA
                     GDSPSGVFTHPVFNTPRLLNSEVVKFLNPFVTQNPQLLLQIIDRLWREPEVELRELAA
                     QLLGKLPSSHYPEVLARIQAWSQVETDADLFPFLHQQASAAIRQQSPELWLEVLNGWI
                     SSNEPWLTKLALAGTIPLIDDYSYENLPAIFAFINPLVLNPGPQNQYELLRVIEHLAR
                     RSEVETVYFLKQVMSLSDAPGLGRFLRRAIELFSPAMQESLRVEMRQRQLT"
     gene            complement(14586..16019)
                     /gene="proS"
                     /locus_tag="PLW25_04375"
     CDS             complement(14586..16019)
                     /gene="proS"
                     /locus_tag="PLW25_04375"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOS53757.1"
                     /translation="MSNEKLPSRAENYSEWYNQVIQRAELADYAPVRGCMVVRPYGWS
                     LWENIQQALDSRFKATGHVNAAFPLFIPMSFLEKEKEHVEGFSPELAVVTIGGGEKLE
                     EPLVVRPTSETIIGYMYSKWIKSYRDLPLLINQWGNVVRWEMRTRLFLRTLEFYWQEG
                     HTAHATAKEAEEETLRMLDIYTRFAVEDAAVPVIPGRKSNSEKFAGAVHSYSIEAMMG
                     DTKALQAGTSHLLGQNFAKAFNIQYLDMNNELQYCWTTSWGVSTRFIGAIIMTHGDDQ
                     GLILPPRLAPIQVVIVPIYKNDEERARVMPVVDKIRAALGSIRVKVDDRSELTPGFKF
                     NDWEMRGVPLRLEIGPKDVEKGSVMAARRDVPGREGKSFLPQENIASSVGALLDTIQS
                     SLYEKARAFRDSHITDVTNYEALTTAVQDGWAHAWWCGSADCENRVKEETKATTRCIP
                     MQQPGGTGKCAVCGRDANEKVYFARAY"
     gene            complement(16016..16576)
                     /locus_tag="PLW25_04380"
     CDS             complement(16016..16576)
                     /locus_tag="PLW25_04380"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOS53758.1"
                     /translation="MNIAVFGGASVRPDDVVYTETLRLGKMLGGHGFTVLTGSYIGVM
                     EAASRGANEAGGHVVGMSCAEIENWRQVKPNRWVLEEHHYETLRERLFALIDGCDAAI
                     VMPGGVGTLSELVVMWNEMIINSKPPRPLIVVGSAWQRVMAQFFASLGDYIGVKDREL
                     IQLVPDVDTAVKILVDYQNQSIRESL"
     gene            complement(16573..16902)
                     /locus_tag="PLW25_04385"
     CDS             complement(16573..16902)
                     /locus_tag="PLW25_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOS53759.1"
                     /translation="MDISQLTTEMNRFVSAKGWYAENSPRPQTARNLAISLVLEAAEV
                     LELYQWGESAVSNQELSDELADVALYLLQIASITGIDLEEAIMDKLKKNFQRSWDGAG
                     TERKEIK"
     assembly_gap    17000..17099
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17100..19172)
                     /gene="fusA"
                     /locus_tag="PLW25_04390"
     CDS             complement(17100..19172)
                     /gene="fusA"
                     /locus_tag="PLW25_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOS53760.1"
                     /translation="MKEYSTEKIRNIVLASHSGAGKTILCEAFLHATGATTRMGRIED
                     GTTVSDFDEEEIRRTISLSTSVIPVEYRDVKINVLDTPGYTDFIGEVISALRVADGAV
                     LLIDAVNGLEVGTEIAWDYCTRFNLPRFLLVNKMDRENANFRKALQTVEDYSETRLIP
                     IQLPWGEKQDFKGVIDLLSMKAYKAQGKTAEEIPADMKDAAEEARMKLVEAAAEGDDV
                     LLEKYLESGSLTEEEVIRGLTLVVQSGDFVPVLVSSGSNEIGVIRLLDAMVDIMPSPL
                     SAAKIVAQGKAGEEILEPKDNAPLAIYVWKTTADPFVGKQTFLRVYAGTISPDARVWN
                     QNKGIEERLGSVNVPRGKESLPVKVIHAGDICVVPKLTETSTTHTLCDKNHPLTLPVP
                     EYPTSLYQVALSPKTQADSTKISSSLTRLCEEDMTLSWHFQTATKQTILQGMGDQHID
                     VAIRRAESKFQVGILMEEPKVPYQETITKSASAMYRHKKQTGGSGQFGEVHLKVSPLT
                     DKEFDFTWDVFGGAISQSYSSSIQKGIQGVMKEGVIAGYPVSGVLVSVYDGKEHPVDS
                     KPVAFEVAGREAFKLAIKDAAPVLLEPIMLVKITVPEAAMGDVMGDLNTRRARIQGME
                     TQKGRSVVTANVPLTEMLRYTTTLRSMTGGRGVFSMEFDHTEVVPTHLAQAVIDAHTK
                     EMESRKEE"
BASE COUNT         3929 a         4878 c         5361 g         5030 t
ORIGIN      
        1 tgatgctgaa cgcggcgaac atcaatcttt tgtctttttg gcgctacctg cacgcaccgc
       61 gtggagacat ggatggcttg atcttcgcgc tgattgtctt tgtggttgca gcggctgagg
      121 tggctgtggg tttagggctt atcgtttctg cctacaggag acgacgcaca gtcgttgcca
      181 acgaacttga cctattgaaa ggttaaacaa tttttcgcat gttcaatatt cacccgctca
      241 tctggctgat cccgctgccg cccttgcttg ggtttttatt gatcgtgctt tttaccaggc
      301 ggtctcatcg agccagtcac agcgtggcgt tggctggggc aggcctttca tttgttggga
      361 gcatggtggt tttcattaaa gcggtcacgg ctggaggtgt ggcggattct tcctttgcgg
      421 catctatcaa ctggctgccg acgggtgcga gctggctgag gattggggtg ctcgtagatc
      481 ctttaaccgc agttatgctc ttttttgttg cctggactgt gctgatgatt ttcatctaca
      541 gcatcggtta ccataacttt ggccagccta tcggaaaaca ggaccgtcca ggtctcccgc
      601 cgcagggggc tgagataacg gaagcgggca aaatccaccg ggtgccctcc gttgagccga
      661 tgtacgcgcg gtttttcgcg ctattaagcc tgtttgcttt tgccatgtac ctgctggtgg
      721 tgagcgataa cctgctgacg ctgtacatcg gctgggaaat catgggcttt tgttcctact
      781 tccttattgg tttctggtac ggcaaggagt cggcgcgcaa agctggcatt aaagctttca
      841 tcaccacacg cataggtgat gtgtttatgt tgctggggat ggtggcgctg tactcagtga
      901 ccgggacatt gaactttcat gaaatattct ctaaccccga gacgatgacc ctgctcacaa
      961 gcacgctctc accggtgctg gggctcaact gggcgggatt gatcggcttg ctgcttttct
     1021 gtggcacgat cggcaaatcc gctcagtttc cgctgcatgt gtggttacct gacgcgatgg
     1081 aagggccaac gccggtctca gctatgattc atgccgcgac gatggtttca gccggagttt
     1141 atctgagcgc gcgtatgttc ccgttgatgt cggcaggatg gcaccatggc gcttctttga
     1201 ccgtgcccat gatggcgatg gcgttgatcg gcggatttac cgcgcttttc acctccacca
     1261 tcgcgctggc gcaaaatgat atcaagcgcg tactggcgta ttccaccatt tctcaactgg
     1321 gttatatgat tgctgcgctg ggtattggcg ctttcaccgc agccgtgttt cacctggtga
     1381 cacacgcatt cttcaaggcg ctgcttttcc tggggtctgg ttctgttatt cacggaatgg
     1441 aacatggcgc gctgcacacg ggcaaacaca ttgacgcgca ggatatgcgc cacatgggtg
     1501 gtttgcgtaa aaagatgcca gtaacctttt ggacattctt agcgggcggg ctggcgctct
     1561 caggtttccc gctcatcacc gccgggttct ggtcgaagga tgaaattctc agcggcgcat
     1621 ttggtggtgg acatctcgcc ttgttcatcg tcttggctgt caccgcgctg ttgacggcat
     1681 tctacactgc acggcagatc acactggtgt tcctcagcaa accaagatct gatgccgccg
     1741 cttatgcgca tgagacaaaa ccggtgatga cagctccgtt ggtggtattg gcggtgttcg
     1801 ctgtgacctt cggttgggtt ggcattccgc acgctttccc aggattgggg gcaatctcgc
     1861 cggcctggtt ccaatctttt gtgggttcga tgttgagccc aggaagtgtt agtgaagccc
     1921 acagcaacat tcccctggtc gtctcgatcc tggtttcgtt gggcgggtta ctgctgggtt
     1981 ggctggttta tcgcggatac gaatctgcgc aggagccaga cccgctgcaa aagcggtttg
     2041 gaggtgtctt taggctgctg gagcacaaat actggataga cgagctttac cagaaggtgg
     2101 tgatcaaccc ggtgttgtgg ctggcggata gagtgattta ccagttcatt gatctgcgtc
     2161 tcatcgatgg tttcctgcac ggtttagcag cggctggcgt ttggctgggg aacttgctgc
     2221 gctcgaagtt tgatctgcct gtggtcaatg gcgcgggcga caacctggca ggtggaaccc
     2281 gcgggctggg tgacttttta cgcaggctac aaaccgggcg tgtgcagcaa tacctggcga
     2341 ttgccgttgc ctgcacggtg gtggcggcgc tgatcatttt atcttacctg gcaaaggtgc
     2401 aagcagggtc tggatgaaca tcctctcagc gcatctcctt tcactcatcc ttttcctgcc
     2461 cctggtgggg gcagtggttc tgctggcgtt cccggccaac aaagttaaag cgatcagagg
     2521 tgttgcgctg gtggccagcc tcatccctct cgccctgacg ctcgtcctct gggggtcgta
     2581 cagcacggcg ctcacggttg gcgagttcaa atttgtagaa aactatccct ggttcacgtc
     2641 catcggctca agctaccatc tggggattga tggcgtttca ttggtgatgc tgctgcttac
     2701 cactttattg accccgctgg ccatcatgac atcctttaat atcaaggaac gcgtcaaggc
     2761 gtttatgatt ttattcctgg tcctcgaaac gggaatgctt ggggtgttca tggcgctgga
     2821 cctggtgctg ttcttcctct tttgggagat tggcctggtg ccgatgtact tcttgatcaa
     2881 tcaatggggc ggagaaaaac gcaattacgc ttcgcttaag ttcatcctct ttaccatggg
     2941 tggttcgctt gggctgctct tagccattca gatgattgga gtggtctgtg gaacctttga
     3001 cctggtaacg atcactgcca gctggccggc gctgcaaggg ggtgagctgt ttggaattcc
     3061 catcgctaca gtaaaggcca ttgccttttg ggcgttcgtg gttgccttcg ccatcaaggt
     3121 gccagtttgg ccgtttcata cctggctgcc cgatgcgcac accgaggccc ccacgggagg
     3181 ctcgatggtg ctggcaggcg tgcttttaaa acttggcggt tatgggttca tccggctggt
     3241 gctgccactt ttcccggctg aatcagcgcg ttttgccagc tggctggcgg ctctggccac
     3301 ggcagccatc atatttggcg cactggcggc tgccgcgcaa acagatttca aacggttggt
     3361 ggcttattct tcgatcaacc acatggggtt tgtggtactg ggtatcgccg cagcggcctg
     3421 ggcaggtgga cagacgaatc ccaccttgcg cgagagcgcg gtgattgcgc ttaacggagc
     3481 ggtattacag atgtttaacc acgggctcac ggctgccggt atgttctttt tggtgggcgt
     3541 gctgtatgac cgtgctcata cgcgccagct ggccgaatac ggcgggctct ttgccatcct
     3601 ccctgtatat ggagcgattc ttgtttttat ctcgatggct tctctgggtt tgccggggtt
     3661 gaatgggttt gtatcagaat ttatgatcgt acgcggtgtg tggcctatct tcaccctgtt
     3721 tatcgcactg gcgatgatcg gcctgctatt caccgctgtg tacgtgctca aggcgctcaa
     3781 attgaccctg catggggcgt tgaacgaaaa atgggttggc aaactgactg agatcagccg
     3841 gcgcgaactg gtggtgatca taccgctgat gctcctggtt gtgatcattg gttggtggcc
     3901 cgcctggatt ttaaatctga tcaatcaaac cgtgatgacc tggttttaat aggagataga
     3961 tgccagtccc gcttctcagt attctcgccc tgaccccgct tgctgccggt attattgtgc
     4021 tgctactgcc ggctgaaaag cgcagtctaa tacgcgggat cgcgtttgct acttcgctgt
     4081 ttacgctggc ggtttctgtc atcattttct tccgtttcga taccaccgcc ggaggttacc
     4141 agtttatcga gaagatgaac tggctgcccg cgttaggcat ctcttaccac atgggggttg
     4201 atggcatcgg tgctgccatg gagctgatgg ctggtattgt tgtgtttagc ggcgtgatgg
     4261 tatcgtggcg ggtggaggac cgaccgcgcg agttctttgc tttcctgttg ttcctggcgg
     4321 caagcgtttt tggcgtgttc gcttcgcttg acctgttcca gctgttcttc ttttttgaat
     4381 tggcggtatt tccgaaatac ctgatgattg tgatgtgggg ctccccgaag acgcgcgagt
     4441 atggcggcat gaagctcacc ctgtaccttt tcctgggttc ggtggtggct ttgatcggtg
     4501 tactggcggt ttacttcact tctgggctgc gtagttttga tatgctggca cttgaacagg
     4561 cggggttggc gctggaattt caacgcgcct ggttcctggt catttttgtg gggtttggtg
     4621 tattgggcgg tctgtttccg tttcacagtt gggcgcctga tggtcacgtg gctgccccca
     4681 cggctgtttc aatgttgctg gcaggcgtgg aaatgaaagt gggcgttttt gccgcgttgc
     4741 gcgccgggtt aatgttgctg ccggaaggta tgcgttattg ggcgccgctg atcctggttt
     4801 tggcgacaat taacgtggtg tatggcgcgt tcatcgccat gatacagaaa gactttaaat
     4861 acgttattgg tttctcctca gtatctcaca tggggctggt gttcattggt tttgccaccc
     4921 ttacgcgaga aggcctcacc ggcgccgggc tgcaaatgtt ctcacatggg atcatgaccg
     4981 ctctcttctt tgctgttgtg ggtatggttt atgaccgtac ccacacccgc gatatccgtg
     5041 aactgggcgg gctgttccgt accatgccga ttgccatgct cggctttgtc attggtggcc
     5101 tggtttccat ggggatgccg gggttctccg ggtttatcgc tgagatgccc atctacatgg
     5161 gcgcctggca aaccgccccg attgtggcca tcatttcggc actttccatt gtcatcaccg
     5221 cggcctatat cctgctcgtt gtcaggcggg tcttttttgg tgaaacgccg gagggcatca
     5281 gagctgggct aagtgatatt tcgcttatgg ataagatcgt tgtggtcctg ttatcgctga
     5341 tccttattgt catcgggttg ttcccgtccg tgttggtgcc ctggatcgaa gccggcgtca
     5401 ggcacatact ggtgctcaca ggaggtgcgt aatgccggcg atcactgctg aaatgctcct
     5461 ctcattatta cctgaactca tcctggctct gctggccttg ttcttattgg cactctcgtt
     5521 gtttaaaaaa gaaaacctgg gcggggctta cagctgggtc acggcgtttg ggtttgggct
     5581 caccctcttc ctgacggtgt tttattcacg cccccctgca gaggttcaac tgctttgggg
     5641 tgaaatgatc cgcttcgacc gggttggttt catcttccgg ttgttgatcc tcgctggcgg
     5701 cgcgatgacc gctatttttg ctggcatgga acaaaccatc gcgaaaaaag ccgagtttta
     5761 cctcttgctg gttctttctt ctatcggttt ttcgctgatg gtttcagccg gcaacctgat
     5821 tatgttgtac ctggcaattg aaacagctac catcccgctg tatgtgctgg ccgggtttaa
     5881 acagcgggat gaaaaatcag ttgaatctgg gatcaagtat tttctgttcg gcggcgtttc
     5941 ctctgctatc ctgttatacg gtttcacact gctttacggc ttcgctggaa cgacccgtct
     6001 tgatggcatg ttggaactga taaaacagaa cggagcgccg atcgcaggga tcaccctagc
     6061 gtcgctgctg gtactggctg gtttcgcttt taagatgagc gccgcgcctt ttcatttttg
     6121 ggcgccggat gtatatgaag gctctccgac gccagttgcc gggttcctct ccaccgtttc
     6181 aaaagcggtt ggttttggcg ccttcattcg cgtactggtg aatgcttttg gtgtgggttc
     6241 ttcgaatgcg tggatggtca ttgtcgccat cctcgccgcg gcgagtatgt tccttggcaa
     6301 tttgttggcc atctcgcagc gggatatcaa gcgcctgatt gcttactcat ctatcgccca
     6361 ggcagggtac atgttggtgg gggtggccgc cggcactgaa ttcggtttca gcgggactgt
     6421 ttattacctg cttgtttacc tggtgaccaa cctggcggtt tttgccatca tcagttgggt
     6481 tgaaaaggga tcgggctcct ccgcagtcag cgcgtttgcg ggtttaaacc gccgttctcc
     6541 ggggatggct ttggtgatga tggtcgcgct cctctctctc ggaggtattc caccgtttgg
     6601 cggtttcttc gccaaggtgc tggtttttgg cgcggcggtg caatcgcatt acgtgtggtt
     6661 ggcggtgctc ggtattctaa attctgtcat cgccttgtac tactatttga agataatgaa
     6721 ggtgatgtat ctggataaac cagatgagac cagctggaag gtgactccag ctttacagtg
     6781 gagggtggcg ctcgcgcttt gtattgcctg tattattctc ctgggtgtta tctatgctcc
     6841 atggctcaat ggcatatccc tcgcggtgac agggttttag ataaagttgg cctttccagg
     6901 cggtttatca atagcaaaaa aacctctatg atataatcga aaatcatgtt tgacacggaa
     6961 agagacaacg aaacaaggaa gacaaatcgg cgcaaccttt ggctggcagc gctcgctggc
     7021 caggtcggct tcttaacttt aatcattatt ttggcagcgg tggtgggagg gctggctctg
     7081 gatgcgcgat tgggcacccg cccctggttt actatcggtt tgctgctggc gagcatcccg
     7141 gtctctctgc tgctcatggt ctacatcacg cgcaaaacgg tcgccaaaat aaaggcgaac
     7201 cacaatgatc acctcaatga ggaggggcgg cttggaaaat aaacgtaaat ggcgttgggg
     7261 aaagcatcgc tggatcctgc tcctgttaat catcctgagc gtaattgggg ttaacctcat
     7321 cgctccggtg cagccgcata ttcaggtggc agccgaaaac ctcagcgaaa aacctctgtt
     7381 caccctgcct gtggtgggag atttttacct gacgaacacg ctcacggcga tgttgttgat
     7441 ggatatcgtc atcattcttg tagcgcttgg ggtgcgccgc gcggcgaaga aaaatgaact
     7501 cgcgccaggc ggggttggta gtgtcttcga gatgctagtg gagatgcttt acaacctcac
     7561 agaaaatacc gccggtaagt atgcggcgaa gattttcccc tggtttgcca ctattctgat
     7621 tgtggtgttg tttgccaact tgttgaaaat tttgcccggc tttgaaacca ttggttttct
     7681 tcatcccagc gagcacgggt tcgccactca ggcattgggg ggcggctggt ttactttgaa
     7741 aaacaccccg gcggcagatg gctatgtgct tgctccattc ttgcgcgggg tttcaactga
     7801 cctgaacttc accaccgctt tggcgctgat cgctgtattt atgattcagg tgattggtgt
     7861 gcaggcgaat ggagcgcgct atttcgaaaa attcatcaat gtgcgcacga tctttaagaa
     7921 accgttgttt ggcgtcatcg attttatcgt tggtttgctt gagctgattt cagaatttgc
     7981 caagattctt tcattcacat tcaggttgtt tggaaatatg ttcgccggta tggtgttgtt
     8041 ggctgttgtg ggtgtgatgc tgcccgtgtt catgccgtcg ttcattatgc tgtttgagtt
     8101 gttcgtcggt gttatacagg cgtttgtatt tgggatgctc accatggtct ttatggcgca
     8161 ggctaccatc acgcataatg ctgaagcaga gcactaaatt ttcaatttat tcaagaaggt
     8221 aagtaatcaa ccaggaggca agaaaagaca tgaatggcga tattgtagaa gcagcaaaaa
     8281 tgattggagc cggcattgct atgattggcg cgcttggcgg aggcattggc gtggggttga
     8341 gcgttcaagg agcggtgcag ggcatggcgc gcaatcctga tacttatggc aacctgttat
     8401 ccagtatgat tcttggaatt gctttttcgg aagcgattgc gatttattgc ctggtaatcg
     8461 cctttctgat gctctttgtg gtataacccg ttgctttcaa tgaggaggtt gctttggaaa
     8521 aactaggtct gaatctcggg ttcctgatcg ttcagataat taactttctt gttatcctgg
     8581 tcgttttacg ggcgtgggtg tataccccgt tacagaattc attgaagaaa cgccgtgaaa
     8641 ccattgaaaa aggactggaa gacgcgcggg ttgctgccga agcccgagaa aacgctgaac
     8701 gcgacgcggc gggtattctg gcagaagcgc aaaacaacgc ggctcagctg atccgggagg
     8761 ccaaagaaaa agcggagctt gccgggcggg aggtgaaagc cagtgtggag gctgatattg
     8821 ccaagaccag gcgcgaagca gcggttgagg tagaacgcga acgtgaacgc gtgcttggtg
     8881 aaatacgcac caatgttgct gccctggcca tcgccgcggc acagaaattg gtcggcgaag
     8941 ccctggacga gaaacgccag catgagctgg tggcggaatt cttctccggt atcaagggcg
     9001 ggaaggtcac catccttgaa gagaacgaat tgaaaggcaa aaacgctgaa gtggtgagcg
     9061 cgctgccttt aacgcgggat gagcagaaag tcatacaggg ggagatgctt tcaaagctgg
     9121 gcgaaaccgc ggcagtcagt ttcaatgtgg atccaaaatt attgggcggc ctggttgtgc
     9181 gtgtaggcga tactgtgatc gacggttcag tcgccggcca gattcagagc ctgcgcgcca
     9241 cgctcgagta agcagaaagt cgtgagagtc ttcctgaacg ccactccaag gagggctctt
     9301 tttatttaat gttagaatgg aaaattgatg aaaccggttt tactctccga tctttttaag
     9361 gaattcccga tcccatgggc aacagacgta gcgcctggcg cgattcccat ctctgccatt
     9421 tattatgatt ctcgaatggt gagaagaggg gcgttgttcg tggcgataag cggcggcagc
     9481 gtggatgggc atgagtacat ttcttcggcc atttcaaggg gagcggtggc tgtggtgggc
     9541 accagagaaa tcagaggcct gcctgtacct tacatccgtg tagcggattc ccgcgaagcc
     9601 ctggcgtacc tggcagcagc gttttttgac caccccgcgc gccagttgac cgttattggg
     9661 gtaacaggca cagatggcaa aacgacaacc gctaatatga tttatgaaat cttaaagcaa
     9721 gccggtgtta aggctgggct tatctcaacg gtgaacgcgg tgattggtga cgaggtggtg
     9781 gacaccggct tccatgtgac cacaccagaa gcggtggaag tgcaaggtta cctgtcgagg
     9841 atgctggcgg ctgggctgac aactgttgtt ctggaaacca catctcacgg ccttgcccag
     9901 caccgggtga ctgcttgcga gtatgatatc gcggttgtta ccaatattac gcatgagcat
     9961 ctggattacc acggtacgta cgaggaatac agagctgcca aaggacgtat gtttgccagc
    10021 ctggcgcaga ccgttgaaaa acctttgggt aacccgcgcc tggccattct gaaccgtgat
    10081 gatatttctt atgactatct tgctggattg gtaaaaggtc gtcaggtcac ttatggtctg
    10141 cagcataaga cggattattt cgcccaggat gttaccagca acccatcagg tgtggatttc
    10201 accctcattg ctgaagggct ggttgaacgt gtgcactgca accttcctgg taagttcaac
    10261 gtctccaatt gcctggcagc gtttgcggcc gcgcgcgcgg caggagttga aacaggtgcg
    10321 gccataaagg gattgtcaag gcttcagggt gtgcctggga gaatggaagc cattgagatg
    10381 gggcagcctt atagcgcgat tgtggatttt gctcacacgc cgaacgcgct caaggtggcg
    10441 ctggaaacag cgcgcgagat gaccacaaaa cgcgtgatcg ctgtgttcgg ttccgccggc
    10501 ttacgcgacc gccagaagcg ccgtttaatg gcagaggttt cagttcaact ggcagacatc
    10561 tccatcttca ccgctgaaga tccgcggacg gaatcgttgg agagtatatt ggcggaaatg
    10621 gcggacgcag ctactttggc tgggggcgtc gaaggaaaaa cgttctatcg cgtggctgac
    10681 cgcggcacgg ccatccggct gggggcggaa cttaccgcgc caggcgacct gttgattgcc
    10741 tgtggaaaag ggcatgaaca atcgatgtgt tttggcacga tcgaataccc ctgggatgac
    10801 cgtactgcca tgcgcgccac gctggcagag caacttggtc tgcctggcta cgaaatgccc
    10861 catctaccta cttcataacg atttttctgt gtaaagaaaa aaggtccggt tggcaaaccg
    10921 gacctcttat atgcttacag ccttagtgcc tgggattgcg attcccgttg cggtcgctaa
    10981 aaccacgatt gtttctgcgg tcgctgctac caccgcgacg atcaccaccg gagcgcggcc
    11041 tgttgccgcc aggttttccg cctttatcgt gctcgagggc ttcttccggt gtccatcctt
    11101 ccaacaccgc ctggcgagat aagcggatct tgccgttttc gtcgatggcg gtgaccatta
    11161 cggtgagctc atcgcccagc ttgaccacat cctcgacccg ttctacgcgt tcgctatcca
    11221 gttgcgagat atgcaccatg ccgtcgatac ctggcagtat ttgcacaaac gcaccgaaat
    11281 ctgccacacg cacgacctta ccggtataaa tatgcccgac ctgggcgctt tcggtgagcg
    11341 cctcaatacg ctcacggacg gtggcttcgc tggccccatc agtagcggcg atatagacag
    11401 tgccatcgtc atcgatgtca attttggtgt tcgtctcttc ctgcagcgcg cggataatct
    11461 tacctcctgg cccgatgatg gcacctattt tatccacggg tatatggagg gtggtgatgc
    11521 gcggcacgaa ggaacgcagt tctggtcgtg gaaccgggat gacttcaagc attttatcca
    11581 ggatagcaag gcgggcttca tgagcctgag tgagtgcctg gcgcatgatc tctggggtga
    11641 tacccttgat cttaatatcc atttgcaagg cggtgatgcc cttggtggtt ccggcaactt
    11701 taaaatccat gtcgcccaga tggtcttcag cgccctgaat atcggtgagc accacatgac
    11761 ggtcgccttc tttgataagc cccatcgcca caccggcaac cggcgctttg atgggtacgc
    11821 ccgtatccat taatgccagg gttgagccgc aaactgaagc catggaagat gagccgttgg
    11881 aggcgagcac ctcagatacc aacctcagcg tataggggaa atctttttca tcgggaatta
    11941 caggaacgag cgctctttct gccagtgcgc catgacctac ctcgcggcgg gactgcccgc
    12001 gcagcatttt cacttcaccg gtggagtacg gcggaaaatt gtaatggtgc atgtaacgtt
    12061 ttgagtccgt gggagaaagg ttgtcaatct cttgcgcttc tttgggtgtg cccagggtgg
    12121 ccattgtgag cacctgggtc tcgccgcggg tgaaaagacc cgagccgtga gcgcggggac
    12181 tgacggcgac ttcgcaccag atcgggcgga tttcattcaa tttgcgcccg tctgggcgtt
    12241 tgttttgctc aaggatacgc gctcgcacaa cgcgcttgta ggtttcttca aacgcttctt
    12301 tatacgcgtt gacctcttca gtgttttcag ggtcagtata ttccaagacg atattctcgc
    12361 gcaggttgtc aatctcgtcc tgtaattcgt gtttgctgtg cggggcgtcg aggagtgtat
    12421 tgatctcttc cgcgatgcgg ttgtaaacct tttccaccaa ggtttcatcc agcgaataga
    12481 gcgcgactgc gcgtttttct ttgccgattt cgcgcgccat ttgcagttgg gtttcaacga
    12541 ttggttgaat agcggcatga ccgaattcca gcgcggcgat catggagcct tcatcaatct
    12601 catttgaacc agattcgacc atgaggatgg catcagcggt accggcgatg cgcaaatcaa
    12661 ggtcagattc ttccatctgg ctgtaagtcg ggttgagcac gaactcacca ttgacacgac
    12721 caacacgcac cgcgccaacc ggcccatccc agggaatatc agagatcatc accgcgcagg
    12781 aggcggcgtt aattgccaac acatccagcg ggttttccca atcagcggag agcgaaaaca
    12841 tgatgacctg aacttcgttg cggatggcgt tattgaacag cgggcgcagc gggcgatcgg
    12901 taaggcgggc aaccaggatg gcatcggtcc ccggtcggcc ttcgcggcgg aagaatgaac
    12961 ccgggatacg cccgccagcg tacatgcgct cttcgtattc cacggtcaac gggaagaaat
    13021 cctgaccgac acggggctca ttgcccatgg tggctgcagc aaagaccatg ctgtcgccaa
    13081 actgaacggt cacagcgccg ccagcctggg cggcgagttt accggtttcg aacgtcaaag
    13141 gacgcccacc aacggtggta ataaatttct tgctttctgg tttcataatt tctcctaatg
    13201 ttcttttccc gcctggttag gaactgaaaa ctggggaaga cgcgcggacg tcttcttcac
    13261 ttttcaatac ccaaccggcg gtgctaggtt gttccgccct ggatgtgcat ttaaaacgag
    13321 tagaggggtt ctactcgttt tggctcaatc tacctggtgc ggatcttcaa tttttcggta
    13381 atggtagcga agcgggcaaa atcagtgcgg cgtaagtatg ccagcagccg gcggcgctgc
    13441 cctaccagtt tgagcaagcc gcgccgagag gaattatctt gcttgttcgc gcgcaggtgc
    13501 tcggtcaatt gccgtatgcg gatagtgagg atcgcgattt gaacatctgc tgaaccggta
    13561 tccgtatcgt gcaggtggta ctcttcgatc aatttctgtt tttcttcttt cgttaatgcc
    13621 atgacagttg ctccttaagt attttgcaag tcgccagctg gcagcccaaa ccgccaacag
    13681 gtcgagtcaa tgaggcgtat tataccagag ggttttattg gtgcacagac acaaatttct
    13741 gaaacgaaag tgcctttttc taggttaact gacgctgccg catctccaca cgcagcgact
    13801 cctgcatggc agggctgaac agctcgatcg cccgtctcag gaatcgcccc aagccaggcg
    13861 cgtcagaaag ggacattacc tgcttgagga agtaaacagt ttcaacttct gacctgcggg
    13921 caagatgctc gatcactcgc agcagctcgt actggttctg tggtcccggg ttcaaaacca
    13981 aagggttgat gaaagcaaaa atcgctggca gattctcgta actgtaatca tcgatcaacg
    14041 gaatggtgcc ggcgagcgcc agtttcgtca gccatggttc attagacgaa atccaaccgt
    14101 tcaagacctc caaccataat tcaggagatt gctgacggat ggcagcagaa gcctgctggt
    14161 gcaggaaggg gaacaggtcg gcatccgttt caacctgtga ccatgcctgg atacgcgcca
    14221 aaacttctgg ataatgcgaa ctgggaagtt ttccaagcaa ctgggctgcc agctctcgta
    14281 actcaacttc gggttcacgc caaagccggt cgatgatctg gagcaacagc tgcgggtttt
    14341 gcgtaacgaa aggatttaaa aatttcacca cctcgctgtt caacaacctg ggtgtattga
    14401 agaccgggtg ggtgaacacg cccgagggag aatcgcccgc tggatgggtc agatcggcat
    14461 ggaaaataaa aatctcgcgt agaccgcgtg tgaactcaac agggtcttct atttgcaatt
    14521 ggagaagttt gaacctggcg cgcagccggg agagatcgac ggcaggcatg gttcaggctc
    14581 cctgcttaat aagcgcgggc gaagtacacc ttttcattgg cgtcgcggcc acacaccgcg
    14641 catttacctg ttccacccgg ttgttgcata gggatacagc gggtggtggc ttttgtttct
    14701 tctttgaccc ggttttcaca atctgcgctg ccgcaccacc aggcgtgagc ccagccatct
    14761 tgaacagcgg ttgtcaacgc ttcataattg gtcacatccg taatgtggct gtcgcggaaa
    14821 gccctggcct tctcatagag agaggattgg atagtatcca gcaaagcacc tactgagctg
    14881 gcgatgtttt cctgcgggag gaaggatttc ccctctctac cgggcacatc gcgtcgggca
    14941 gccataacag atcctttttc cacatctttg ggcccaattt caagccgcaa cggaacgccg
    15001 cgcatttccc agtcgttgaa cttgaagccg ggagtcaact cgctacgatc atccacttta
    15061 accctgatcg aacctaaggc agcgcggatc ttatcaacca caggcatgac cctggcgcgc
    15121 tcctcgtcgt tcttgtaaat ggggacgatg accacctgga tgggcgccag gcggggcggc
    15181 aagatcaacc cctggtcatc gccatgggtc atgatgatgg cgccgataaa acgagtagaa
    15241 accccccagg aggtcgtcca gcagtattgc aattcattgt tcatatccag atactggata
    15301 ttgaaggctt tggcgaagtt ttgccccagc aggtgagatg tccccgcctg caatgctttg
    15361 gtatcgccca tcatagcttc aatggaataa gagtgcaccg cgccagcgaa tttttcgctg
    15421 ttgctttttc gcccgggtat cactggcacc gcggcatctt caacggcgaa acgcgtataa
    15481 atatccagca tgcgcagtgt ctcttcttcg gcttcttttg ccgtggcgtg ggcggtgtgc
    15541 ccctcctgcc aatagaattc cagggtacgc aggaagagcc ttgtgcgcat ctcccagcgc
    15601 accacgttgc cccactggtt gataagcagc ggcagatccc ggtagctttt aatccacttg
    15661 gagtacatgt aaccgatgat cgtttcagag gttgggcgca ccaccagcgg ttcttcaagt
    15721 ttttcgccgc cgccgatggt cactacagcc agttcaggcg aaaagccctc tacgtgctct
    15781 ttttcttttt caagaaatga catggggata aagagcggga aagcagcatt gacatgaccg
    15841 gtggctttaa aacggctgtc cagggcctgc tggatgtttt cccataatga ccatccataa
    15901 gggcgcacaa ccatgcagcc acgaaccggg gcataatcag ccaattcagc ccgttgaatg
    15961 acctggttgt accattcaga ataattttct gcgcgcgaag gtagtttttc gttgctcata
    16021 agctttccct tatagattga ttttgataat ccacgagtat ttttacggcg gtgtctacgt
    16081 ctggcaccag ctggatcaat tcgcggtctt taacaccgat atagtctccc aaggatgcga
    16141 aaaattgtgc catcacacgc tgccatgcgc tgccaacaac gatgagaggt ctggggggtt
    16201 tactgttgat aatcatttcg ttccacatca ctaccagttc gctcagtgta cctacgccgc
    16261 ccggcatcac aatcgccgca tcgcagccat cgatgagcgc gaagagtcgt tcgcgtaagg
    16321 tttcgtaatg atgttcttct aaaacccaac gattgggctt cacctggcgc cagttttcaa
    16381 tttcagcgca ggacattcct accacatgac caccggcttc attggcgccg cgcgaggcag
    16441 cctccatcac gccgatgtaa gaacctgtca ggacggtgaa cccgtgcccg cccaacattt
    16501 ttcccagcct taacgtctca gtataaacca cgtcatctgg gcggacagaa gctccgccaa
    16561 ataccgcgat attcatttga tttccttcct ttcggttccc gcgccgtccc atgaccgctg
    16621 aaaattcttc ttcagcttat ccataatcgc ttcttccagg tcaatcccgg tgatgctggc
    16681 gatctgcaga agatacaggg cgacatccgc caattcgtcg ctcaactcct ggtttgaaac
    16741 ggcagactct ccccattgat aaagctccaa tacttcagct gcttccagca ccagcgagat
    16801 tgctaggttg cgcgcggttt gcgggcgcgg gctgttttca gcgtaccagc ctttggcgct
    16861 gacaaagcgg ttcatctccg ttgtgagctg gctgatatcc atgttatcct tcagccttct
    16921 tgactatccg gttgaacttc ctggcgcaca actcttgaat tatcgctctc ttcatgcttg
    16981 actcactatg tggaactcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    17101 tattcttctt tgcggctttc catttctttg gtgtgggcat cgatcactgc ctgggcaaga
    17161 tgggtgggca ccacctcggt atggtcgaat tccatcgaga acacaccccg accccccgtc
    17221 atagagcgaa gcgtggtggt gtaacgcagc atttccgtca acggtacatt cgcggtaacc
    17281 accgaacgac ctttttgcgt ttccattccc tggatacgcg cgcggcgagt gttcaggtca
    17341 cccataacat cgcccatggc ggcttcaggc actgtgattt tcacgagcat gataggttcc
    17401 agcagcaccg gggccgcgtc tttaatagcc agtttgaatg cttcacgccc ggcaacttcg
    17461 aaggcgactg gttttgaatc caccgggtgt tctttgccgt catacacgga taccagcacg
    17521 cctgaaaccg gatacccggc aatgacgcct tccttcatca cgccctgaat gcctttttgg
    17581 atggaggagg aataggattg cgaaatcgcg ccgccgaata catcccaggt gaaatcgaat
    17641 tctttatcag tcagcggaga gaccttgagg tgcacttcgc caaactgacc ggaaccgccg
    17701 gtttgtttct tgtggcggta catggcagaa gcgctcttgg tgatggtttc ctggtaggga
    17761 actttgggtt cttccatcag aatcccaacc tggaacttgc tttcagcgcg gcgaatggcg
    17821 acatcgatat gctggtcgcc catgccctgt aaaatggttt gtttcgtggc ggtctggaag
    17881 tgccaggaga gcgtcatatc ttcctcgcac aacctggtga gagaagaaga aatcttggtg
    17941 gaatccgcct gcgttttggg tgaaagggca acctggtaaa gggaggtggg gtattcagga
    18001 acaggcaggg tgaggggatg gtttttatcg cacagcgtgt gggtggtgga agtttcagtc
    18061 aattttggca cgacacaaat atcacccgcg tggatcactt taaccggcag agattcttta
    18121 ccgcgaggaa cgttcaccga ccccaaccgt tcttcgatgc ccttgttttg attccaaacc
    18181 cgcgcatcag ggctgatggt tcctgcgtac acgcgcaaga aggtttgctt gccaacgaac
    18241 gggtcggcgg tggtcttcca gacgtaaatg gcaagcggag cgttatcttt tggttcgagg
    18301 atttcttcgc cggctttgcc ttgcgccaca attttcgccg cggaaagcgg agatggcatg
    18361 atgtctacca tggcatccag caggcggatg acgccgattt cattagaacc cgaggagacc
    18421 agaacaggaa cgaaatctcc ggattgaacg accagtgtga gccctctgat gacctcttct
    18481 tctgtcaggc tgccgctttc cagatacttt tcaagcagaa catcatctcc ttcggcggcg
    18541 gcttctacca gtttcatccg cgcctcttcg gcagcgtctt tcatatccgc aggaatttct
    18601 tccgccgttt tcccttgcgc tttatacgcc ttcatcgaga gcaggtcaat cacacccttg
    18661 aagtcttgct tttcacccca gggcagctgg ataggtatga ggcgggtttc agaataatct
    18721 tcgactgttt gcagcgcttt gcgaaaatta gcgttctcgc ggtccatctt gtttaccagc
    18781 aaaaagcgcg gaaggttgaa cctggtgcag taatcccagg cgatttcagt gccaacctca
    18841 agcccgttga ccgcatcaat cagcaggacc gcgccatctg ccacgcgcag cgcggagatc
    18901 acttcaccga taaagtcagt gtagccgggt gtatccaata cattgatctt cacatcacga
    18961 tactcaacag gaatcacaga tgtggaaaga gaaatcgtgc gtcgaatttc ttcttcgtca
    19021 aaatcagaaa cggtagtgcc gtcttcgatc ctgcccatac gggttgttgc cccggtggca
    19081 tggaggaaag cttcgcacag gatggtcttc ccagcaccag agtgtgatgc cagcacaata
    19141 ttgcgaattt tttcagttga atattctttc atggaccggg tccttctgtt aatagatgat
    19201 gtaataaccg cataattcta ccggacgatg ctttttctgt caaagacaat caccggaacg
    19261 gttatacacg gtatactgaa ctcagtaagc cagccgct
//
LOCUS       DAOSPM010000032        18940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_2924_length_18940_cov_12.306010, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000032 DAOSPM010000000
VERSION     DAOSPM010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18940
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2924_length_18940_cov_12.306010"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..540)
                     /locus_tag="PLW25_04395"
     CDS             complement(220..540)
                     /locus_tag="PLW25_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53761.1"
                     /translation="MEDKITIIEGPPPVFEKIDDGWALGLTESSAYYGMALTRLRTFN
                     GPALVERCHRRWNRQEPIFLHYRNEMGLEQQVPILAARASEVDEGQVLFLWIRYPMDS
                     HGDE"
     gene            689..1108
                     /locus_tag="PLW25_04400"
     CDS             689..1108
                     /locus_tag="PLW25_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOS53762.1"
                     /translation="MTLYIRTPEMIAESRRRMMRRMLENTFNQERVLSFPLEMKVNEN
                     EYTLTALLPGLSTDEINIQFNNGTLSIDGEYQEIRDEKSEYLFSEMPAGKFSRSVEIN
                     DPIVSEKISAGMKDGVLTIHLPKAEEAKPKTIKINAK"
     gene            1187..1999
                     /locus_tag="PLW25_04405"
     CDS             1187..1999
                     /locus_tag="PLW25_04405"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOS53763.1"
                     /translation="MERHEIAETLRGTAVSAWHHFESIGSTNTEAQCWAESGAPDFAL
                     VLAEQQTSGRGRFDRRWITSPGASLALSLILRPTPDEVPRLALFAPLCGIAVHETVQR
                     VCGLSPQIKWPNDVLLKGQKFCGILVESSWQGSQPAAVIMGIGMNINRGSIPPPELRG
                     FPATSLEAEYGSPVDRAAILRELLFSIHAWRGKLGTPVFMQYWEEHLAFKGQQVRIEN
                     SERPLIIGKIKGINELGQLLLINDSGEMVPVTVGDIHLRPVQPDDPGGNNVG"
     gene            1989..2207
                     /locus_tag="PLW25_04410"
     CDS             1989..2207
                     /locus_tag="PLW25_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53764.1"
                     /translation="MLDDLRNSVNSAYEEELIEEEHIRGGRYTIERPPFLGMSPFQRF
                     IVALVFFLMVAILGILLLVVFQKIMPPI"
     gene            complement(2295..>4414)
                     /locus_tag="PLW25_04415"
     CDS             complement(2295..>4414)
                     /locus_tag="PLW25_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOS53765.1"
                     /translation="DIDKDTVDFTDNFDGSLKEPVVLPARLPNLLLNGSSGIAVGMAT
                     SIPPHNLRELANATNYLIDHYEKIDEVSVEELMRFVQGPDFPTGGIIVGRDSVHQAYS
                     TGRGRLVVRGQAHIEEMKGNRYQIVITEIPYQVNKTSLIERIAELARGGRLDAITDLR
                     DESDRRGMSIVLELRRGAQPKQVLNQLYKYTPLQSTFSVHLLALVNGEPRLLSLKRAL
                     QIYIEHRQVIITRRSSFELEKAKNRAHILDGLLIALANLDDVIKTIRESKDAEIAKER
                     LMSKFNLTDIQAQAILDMQLRRLSALERQKIEDEHREIMARIEYLQDLLAHPQKMLGL
                     IKEDMNAVSEKYGDERRTKIVGETHEELKEEDLITDEPVLITFTRKGYIKRVAARLYR
                     SQGRGGRGVSGQTIREEDEIEFLIPARTLHTVLFFSDRGKVYSEKVYQIPDANRTDRG
                     IPIVNVLSLDTGERITAAVSVPGFEEGSYFTMATLAGRVKRVALADFSSVRPSGLIAI
                     SLDEGDELGWVRLTSGKDEIIMVTRAGQALRIPETEIRCMGRPAAGVTGIKLKTGDRL
                     TSMDVIEPEGQLLTVTEHGFGKRSDLDEYPVKGRATGGVATIAQKQTARVGRITSARV
                     VQEEDEVTLISSGGVILRLKVKDISMSGRATRGFKLMDLGDEDVVASVARIQTSDITK
                     TEGDSLPLENGKAETPGEEPAGV"
     assembly_gap    4415..4514
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4770..5660
                     /locus_tag="PLW25_04420"
     CDS             4770..5660
                     /locus_tag="PLW25_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOS53766.1"
                     /translation="MKQALCSFVLILFLLTGCAGRSHTRPGTLPQPAAVSIENKGSDT
                     MVNLALAWAERYQQVNPNARISVSGGGSGTGITSLISGTVDIANASRRITEEELAAAR
                     QAGYDPHEFTIARDAIAIIVNPRNPVSNLTLQQLSRIYRGEINNWNELGGEDREIVRL
                     SRETNSGSHLFFLESVVRMGEMDNNAIFSASTLLLPSSEGIIAEVRDNPNAIGYDSLG
                     YITSDVKLVGISSLEEPMNFILPSLETVSNQMYLLSRNLYMYTLDEPEGLVKTYLDWI
                     MGPEAQEIAHKLGFMPVLNE"
     gene            5671..6567
                     /gene="pstC"
                     /locus_tag="PLW25_04425"
     CDS             5671..6567
                     /gene="pstC"
                     /locus_tag="PLW25_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOS53767.1"
                     /translation="MKAREEKDGLVINGVIRISGYSSILFVVLILFFLLREGLPALTN
                     IPLRSLFSIRWYPIEGYYGILPLIFGSLLITLASTLIALPFGIGTAVYLSEVAPPWLR
                     DLLKPIVEILAGIPSVVLGFIGILVFAPLFRKMLNIPTGLTAFTGAILLAFISIPTIV
                     SVAEDALNTVPHSYRQASLALGATQWQTIWDVTLPAAKTGVLTAVMLGVGRSLGETMA
                     VMMVTGNAPLMPLHLNSLFRPVRTMTATIASEMGEVASGSLHYQVLFFIGITLFLLSL
                     VINITASLVSTKARQRSERLLS"
     gene            6567..7430
                     /gene="pstA"
                     /locus_tag="PLW25_04430"
     CDS             6567..7430
                     /gene="pstA"
                     /locus_tag="PLW25_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOS53768.1"
                     /translation="MKAAHLTRAKMAQTIGFHLLRLVTLITIIPIIGLVVYIVVKGAP
                     AISWEFLTAAPSNGMREGGIFPAIVGTLYLTLGTALFSVPIGVLAGIYLAEYAQETAF
                     TRIIRIAIINLAGIPSVVYGLFGLGLFVLFLHLGTSILAASLTLSIMTLPVIISTTEG
                     ALRSVPNSFRVVSTSLGATRWQTIRRITLPQALPGIITGIILGLERAAGETAPILFTG
                     ATFFLPQLPRSPLDATMALPYHIFVISTQIPGMPIQIQYGTVLVLLVFVLGMNLIATL
                     IRSRARAKRQW"
     gene            7435..8190
                     /locus_tag="PLW25_04435"
     CDS             7435..8190
                     /locus_tag="PLW25_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOS53769.1"
                     /translation="MKALQVQNLSVRYSDNNESLKNVSMEIESRAITVLFGPAGGGKS
                     TFLRTLNRLNDLADVKEIKGRVLFHQRNILDPEEDVITLRRKIGMVFSRPVPLPLSIF
                     DNVAYGLVMAGEHNQARLGEKVEKALKQAFLWNEVHDRLNQSAFSLSGGQQQRMCLAR
                     VLALEPEVILLDEPTSALDPIVTSSIEDMLRELKKEYTILIAPHSVQQAARLADYAAF
                     FLMGELIEYGSGIEIFQVPKDQRTSDYITGRFG"
     gene            complement(8239..8715)
                     /locus_tag="PLW25_04440"
     CDS             complement(8239..8715)
                     /locus_tag="PLW25_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOS53770.1"
                     /translation="MAEKITFTINGKLTKVEVDPEMLVIDVIRDVLNLTGTKRGCDNS
                     VCGTCTIVVNGKATKSCNMPIKKLAGADVLTVEGLAKGTELHPIQKAISESGAIQCGF
                     CTPGIVMELYGLLEEKPDAAQSEIENALSKHLCRCTGYEPIRDGALMAQKLIKERK"
     gene            complement(8715..11039)
                     /locus_tag="PLW25_04445"
     CDS             complement(8715..11039)
                     /locus_tag="PLW25_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOS53771.1"
                     /translation="MKFKYLGKSYNRPDAISKVTGKAVYLDDIRIPGMLHAAILRPEY
                     AHAEIVRIDKTEAEKMPGVVKVVTGENCHYHYGDNFKDLVPMAVKKVRYIGEPVAAVI
                     AETLAQAEAALEKIKVEYKPLPVYIDARDAMKPGAALVHEENGEYWHLPTLKPTPGTN
                     IAHTYHLKKGKGVDGFKDADVIVEGEFNYPLGSCAAIEPHGSIVWFKEDKTIEVWSSS
                     ICPFIIRDDLAHSYGVPVSDVRVRIPDIGGCFGYKSDITVEQTVAWIASFVPGHPVKW
                     VASRKEDFTSTLVAHGIRSRMKIGARKDGKLVAIQNEVLHTGGAYADTAVNITIAATH
                     NSAGPYEFEHCDLTGYTVYTNTPPVGAYRGYGHQEAQIATERMMDLLARKLNMDPIKL
                     REMNYLVEGKVNALGEKMWKSHGDLMTCSNMVKEKVFSKPKPAEDENYYYGRGFAAVM
                     KSPKGAPFSTKSCYMKMNIDGSISVSMGGAEVGQGLRTVVQQVTAEALKIAPERVRVY
                     TEIDTQYSPYEWQTIGSMFTTQGGRAIVRAADKLIAVLKNTAAQVLKTDVDYLEYDGD
                     YVYLRNDPDIRVAVADLSRGYITSDGITIGEYAHAVSDARLPRYANPDENGQGSLGVN
                     YTFGATAAEIRIEKKTGKIIVDKFTSVFDVGQIINPKQIRGSVMGGVTMAVGAALYEK
                     LDISEDGKINNPQLFKYHIPTYKEAIPMDIEFIETPDAVGPFGARGVGEHSVIGPAPA
                     IMNAIRDAVGIDFFEIPITPDVMKKALEARQEVK"
     gene            complement(11333..14776)
                     /locus_tag="PLW25_04450"
     CDS             complement(11333..14776)
                     /locus_tag="PLW25_04450"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOS53772.1"
                     /translation="MKSKNLVPVSATLIAGLCFAYSLVVRFSHFLLVSRGEKAITLGV
                     HWLWIVFAGLLFILPLVNRLLEGISKERLKIIGGLSLVTAVVIMAVFYSLPAFPKRME
                     LEIRAKGEELAVAGKGITILSMVRQDPPKNIPTPINLRNLSYGGGWQVMEGKLRFSPG
                     QGDSGSVRYTSNIQGEVRIRFQTGPGEGAVDILVNGEGQSVELAEAEEGEVEKTVTIP
                     NTWKNADRVRRVLLMAGLLTDTVTVFLSAVMFWLLVYQVVVKRRLRARGLADVVGLVL
                     VSLVLLFANQQAQREIVFSDPKLEEGVREALGKPEGRLYTHQLLTLIELDLSGRGIRE
                     LEGIEGIRNLRVLDLSDNQIENLTPLQGLKKLQKLNLRENRVRDITALAEMKQLTYLN
                     LHSNEDIQDLTPLKNLTMLNSLILRNVPIGDQVSILTNLNRLTYLNIRHTSIRNIEVL
                     VEMMSHGVLQDDVSAGKRVTVNILENEIGEGKGDPFLGLRGYWEKVSYRYPYYLPNYA
                     SEVAGPKFSHESGFYEEGFALVLTTPEAGGKIFYSTDGSEPAIDEKRMAMGSTREYTG
                     PITVNDKSAGRNRLSQIETSRIDDYKPPENMFKAMVVRAVVVGEDQRRSGVVTHTYFV
                     EEGVKERFSLPVLSLTVAPVTLFDDRYGIYVPGELYQNDHPSNQLHNTANYTQRGLKW
                     ERPAFVELFDPSGEVVFAQEIGIRIHGRASRGYPQKSLRLYAREEYSGESTLQFDFFP
                     GLNERLNRLEVDKFETLILRTGGAGDMEDTMIADIIGQSLLENLQLDIQGNYPVIVFL
                     NGEYWGIHNLRSRYDAGYFQNYYHIPPGDVVVLVGSESELYLGDPGDENLYTDMLRLI
                     DEHYDTNGFRTVSTLADAQAYAAMSELMDMENYIDYNISQIYPNKVDWLGNNIWYWRK
                     KVDCALPESGEMYGHDGKWRWMVIDMDSGFKDRYFNSMENVTAEGSQGTYLFRSLLQN
                     EEFRRSFINRLADLLNTNFKTEVVLATIDEVQRVYEPEMEEHILRWGMPGGSLAGWRA
                     NVEEIREFARARPEELRQQIADYFDLAGSAELRIRTDPGKGYVTVNRLEIREGMVGIE
                     DPSNWAGVYFQGVPFTITAVPAEGYRFSHWEGLKGGDSTAQSQEITLTGDLALTAVFV
                     R"
     gene            complement(14816..15559)
                     /locus_tag="PLW25_04455"
     CDS             complement(14816..15559)
                     /locus_tag="PLW25_04455"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOS53773.1"
                     /translation="MKPSARLDEKNWRFEYKYRLSVEQYLRVRNQIRAYMEIDEYTER
                     AAEKKYLVCSVYYDSLGLDAFNEKIDGNSDRIKFRLRTYCRELSQGTTIRAEMKVRKN
                     AITEKYGAFISGAEYEMFIGQKRWPKIRNQVMEEFERYVHLKGLNPLLLTEYRREGYR
                     SRAREEIRITFDHRVKSAHSRTLFPEAPHFHEHHPGLIVLEIKCIKQQPGWLRRLVQQ
                     NGLMIMANSKYVQGMMVAYPALVTPGWSA"
     gene            complement(15584..>16295)
                     /locus_tag="PLW25_04460"
     CDS             complement(15584..>16295)
                     /locus_tag="PLW25_04460"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOS53774.1"
                     /translation="RRKKGMTIIDRLAGLLDRILISGQDIGDVLFSVLLAAVMGLAIS
                     RIYKWTHRGLNYEHTFMATLVLLAPIVSIVMLYIRGDLVLSLGLIGSLSIIRFRTPIK
                     DTRDMVFLFWVIAVGLGAGTYNWAVVGVTTPIVAVIVAALYFFRYGHSPNTDFVLVVN
                     GSGEYRKEEIENVLEKYTTGKRARSFDVDGEKWEVIYELRFTKREKQPVEGMVAEMKE
                     MSGVNKVSVLAPQLALPL"
     assembly_gap    16296..16395
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16396..16968
                     /locus_tag="PLW25_04465"
     CDS             <16396..16968
                     /locus_tag="PLW25_04465"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HOS53775.1"
                     /translation="RFVFHSLCHPQIESLPNHWLKLRVEEKIKESGLNFTIFQPTPYM
                     QNVLGQRQNILERGVYEIPYQPSTLLGMVDLDDVAEVAARVLLTPKTYDWATYELAGP
                     EILSQTEVLHIIRLVTGKEIALKVISREDWCARAASTGMPQYAVSIFYRMFEFMESSG
                     FWGNPGVLEWLLERKARRFEAWFRAVGNWS"
     repeat_region   17021..18940
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17022..17049
                     /rpt_unit_seq="ggtccatccccacgcgtgtggggaatac"
BASE COUNT         4039 a         5640 c         4523 g         4538 t
ORIGIN      
        1 agagcaaagc actcctaacg cttaggttga gagttcgagt ccctcccggg gcacaaattt
       61 taagattcaa acctcatttt accccttgcc atcgaatacg gactggtgta tattaagggt
      121 aagaaacatt tgggtgcccc cctcacccag atgtttcttg tttttaacga acaaaatccc
      181 ggcgtgatgc cgggcttggt tttgttacaa ctcatcgctt cattcgtcac catgcgaatc
      241 cattgggtag cgaatccaca ggaacagcac ctgcccttca tccacctcgc tggctcgcgc
      301 cgccaagatg ggcacctgct gctccaaccc catctcgttg cggtaatgca ggaagatcgg
      361 ttcctgccgg ttccacctgc ggtggcagcg ttccaccagc gccggtccgt tgaaagtgcg
      421 cagccgcgtc agcgccatcc cataataggc cgagctttcg gtgagaccca gcgcccagcc
      481 gtcatcaatt ttttcaaaga ccggtggcgg tccttcgatg atggtgatct tatcttccat
      541 gtcttccttc ctggcggaat gataccacag ctaggaaacc tcaaagaaga aatgaatatt
      601 aatacgcttt taataatctg tttatcaccc tcttatatcc ggcgcctaaa atgtgtgtaa
      661 atttatgaaa aagatatgga ggaatgaaat gaccctatat attcgcaccc cagaaatgat
      721 agccgaaagc cgccgcagga tgatgaggag aatgctggag aacaccttca accaggaacg
      781 cgttctgtct ttcccgctgg aaatgaaagt caatgagaac gagtacaccc tcaccgcctt
      841 gttgccaggt ctttcaaccg atgagatcaa catccagttc aacaatggca cgctctcgat
      901 cgatggtgaa tatcaagaaa tccgcgatga gaaatcagag tatctctttt cagagatgcc
      961 cgccgggaag ttcagccgca gcgtcgaaat caacgacccg atcgtcagcg agaagatcag
     1021 cgcgggcatg aaggatggtg tgctcaccat tcacctgccc aaagctgaag aagccaaacc
     1081 gaagaccatc aaaatcaacg ctaagtaaaa cgccctgtca ccgaggagat atacaaggag
     1141 agcggtcaat cccgctctcc ttgtattaca tagtagaatt agccccatgg aaaggcacga
     1201 gatagcagaa accctccgcg ggacagcggt tagcgcctgg catcactttg aatccatcgg
     1261 gtcaaccaac accgaagccc agtgctgggc ggagagcggc gctccagact tcgcgctggt
     1321 gctcgccgaa cagcaaacca gcggtcgcgg acgtttcgat cgccgctgga tcacttcccc
     1381 tggcgcctct ctggctctca gcctgatctt gcgcccaacg ccagatgaag tccctcggtt
     1441 ggcgttgttc gcgcccctat gcggcatcgc cgtgcacgaa acagtgcaac gggtttgcgg
     1501 gctttccccg cagattaaat ggccgaatga tgtgctgctc aagggtcaaa aattttgcgg
     1561 catcctagtg gagtcctcct ggcagggctc tcaacccgcg gcggtgataa tgggcatcgg
     1621 tatgaatatc aaccggggtt cgatccctcc accggagttg agggggtttc cagccaccag
     1681 cctcgaagcg gaatacggca gccccgtcga ccgcgctgcc atcctgcggg aattgttatt
     1741 ttccatccac gcctggcgcg gtaaactggg cacacccgta ttcatgcaat actgggaaga
     1801 gcacctcgct tttaaaggtc aacaggtgcg catcgaaaat tcggaaagac ccttgattat
     1861 tggtaaaatt aaagggatca acgaattggg tcagttgttg ctgatcaatg acagtggaga
     1921 aatggtgcct gtgactgtcg gagacattca cctgcgtccc gtacaaccgg atgacccggg
     1981 agggaataat gttggatgac ctgcgcaatt ctgtgaacag cgcttatgaa gaagagttaa
     2041 ttgaagaaga gcatatccgg ggtggaagat acaccattga gcgaccgccc tttttgggca
     2101 tgagcccttt tcaacgtttc atcgtcgcgt tggtgttctt tctgatggtc gctatcctcg
     2161 gcatcttgct gctcgtcgtg tttcaaaaaa tcatgccgcc aatttaatgc gccgcacgag
     2221 ctcatccccc gtcccggctc tctacccggg aaggggttga ttgccttcgt tcattcctca
     2281 ggcgaaggga ttagctacac tccggcaggt tcttcaccag gcgtctctgc tttcccgttt
     2341 tcaagcggca gcgagtcacc ttcggtttta gtgatatccg aagtttggat tcgcgccacc
     2401 gaagcgacaa catcctcgtc tcccaggtcc atcaacttga acccgcgcgt cgccctgcca
     2461 ctcattgaaa tatccttcac tttcaaccgc aagatgacgc cgccggaaga aatgagcgtc
     2521 acttcatcct cctcctgcac cacgcgagcc gaggtgattc gccccaccct cgccgtttgc
     2581 ttttgggcga tggtcgccac accgccagtc gcccgtcctt tgaccgggta ttcatccagg
     2641 tcagagcgtt tgccgaaacc atgctcggtc acggtaagca gctgcccttc tggttcgatc
     2701 acgtccatcg aggttaatcg atctccagtt ttcaacttga taccggttac accggcagca
     2761 ggccggccca tgcaacgtat ttcggtttca ggtatacgca gcgcctgacc ggcgcgggta
     2821 accatgatga tctcatcttt accgctggtc aggcgcaccc agcccagctc atctccctcg
     2881 tcaagggata ttgcgatcag tcctgaaggt ctgacgctgg agaaatccgc cagcgccact
     2941 cgcttgaccc tgccagcgag ggtcgccatt gtaaagtatg aaccttcctc aaagccaggc
     3001 acggaaactg ccgcggtgat gcgttcgccg gtgtccaggg agagaacgtt cacaatgggg
     3061 atgccgcggt ccgtgcggtt ggcatcagga atctgataca ccttttcaga atacaccttg
     3121 ccgcggtcgg agaaaaagag gacggtgtgc agcgttcgcg ccgggatgag gaactcaatt
     3181 tcatcttcct cgcggatggt ctgcccagaa acaccgcgcc cacccctgcc ttgagagcgg
     3241 tagagccttg ctgccacgcg cttgatatag cctttacgcg tgaatgtaat cagcacaggt
     3301 tcatcggtaa tgagatcctc ttctttcagc tcttcgtgag tttccccaac gatcttggtg
     3361 cgccgttcgt caccatattt ttctgaaacg gcgttcatat cttcttttat taaacccagc
     3421 attttctggg gatgcgccaa caggtcttgc agatattcga tgcgcgccat aatctcacgg
     3481 tgctcatcct cgatcttttg ccgctccagc gcggataacc tccgcagttg catatccaga
     3541 atcgcctgcg cctgaatatc cgttaaatta aatttcgaca tcagccgttc tttggcgatt
     3601 tcagcatctt ttgattcgcg gatggtttta atcacatcat ccaggttagc cagggcgatc
     3661 aataaaccat ccagtatatg cgcgcggttt ttcgctttct cgagctcaaa actcgaacgc
     3721 cgggtaatga tcacctggcg atgctcaata tagatttgga gcgcccgttt taaggaaagc
     3781 agccgcggct caccattcac caacgctaat agatgcacag aaaacgtgga ttgcagcggg
     3841 gtatatttat acagttgatt cagcacctgc tttggctgcg ctccgcgccg cagctccaac
     3901 acgatgctca tgccgcgcct gtcagattca tcacgcaggt ctgtgatggc gtccagcctg
     3961 ccgccgcgcg ccagctcggc aatgcgttcg atcaggctgg tcttgttcac ctggtacgga
     4021 atctcggtga tgacgatctg atagcggttt cctttcatct cctcaatgtg cgcctgaccg
     4081 cgcaccacca gcctgccccg tccggtgctg taagcctggt gtacgctgtc tcgaccgaca
     4141 ataatgccgc cggtggggaa atccggtccc tgcacaaaac gcatcagttc ttctaccgaa
     4201 acctcatcga tcttctcata atggtcaatg aggtaattgg tggcattggc cagttcgcgc
     4261 aggttgtgcg gagggatgct ggtagccatg cccaccgcga taccgctcga gccgttcagc
     4321 agcaggttgg gcagccgcgc cggcaacaca actggttctt tcagagaacc atcgaagttg
     4381 tctgtgaaat cgaccgtgtc tttatcaata tcggnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnatgcgg cggtgcaccg gtttcagtcc gtcccgggca tccggcagcg
     4561 cgcgtgagat aatcacgctc atcgcataat cgaggtaagc ggagcgcatt tgctcgtcaa
     4621 tatccacctg gtgtactgtt ccgaaatcca taattcaacc tttcctgtgt tatcagccgc
     4681 atgattatac ctcattcaat ttttacgctg ttggttccag cctgggtgtc cgtcaataac
     4741 ctggtcttta aggtagaatt gaaaaatcaa tgaaacaagc attgtgttca tttgtgttga
     4801 tcctgttcct gctcacgggc tgcgccgggc gttcgcatac ccggcctgga accttgccgc
     4861 agccagccgc ggtatccatt gagaataaag gctctgatac catggttaac ctcgctctgg
     4921 cctgggcgga gcgctatcaa caggtcaacc caaacgcgcg catctcggtc agcggaggtg
     4981 gctccggcac cggcatcacc tcgcttatca gcggcacggt ggatatcgcc aacgcctcac
     5041 gccggattac agaagaagaa ttggcggcgg cgcgtcaggc tggttacgat ccccacgaat
     5101 tcaccattgc ccgtgacgcc atcgccatca tcgtcaaccc tcgcaacccc gtcagcaatc
     5161 ttaccttgca gcagctttct cgtatctacc gcggagagat caacaactgg aacgaactgg
     5221 gcggagaaga tcgcgaaatc gtccgccttt cgcgcgaaac caactctggc tcacatctct
     5281 tcttcctgga atcagtggtg cgcatgggcg agatggataa caatgccatt ttttctgcga
     5341 gcaccttgct gctcccctct tctgaaggta ttatcgctga ggtgagagat aaccctaatg
     5401 ccatcgggta cgacagcctg ggttacatca ccagcgatgt caaactggtg ggaatatcct
     5461 cacttgaaga gccgatgaac ttcatcctcc catccctgga gactgtcagc aaccaaatgt
     5521 acctcctttc gagaaacctt tatatgtaca cccttgacga acctgagggg ttggtgaaaa
     5581 cctatctgga ttggatcatg ggccctgaag cgcaggagat cgcgcacaaa cttgggttca
     5641 tgccggtttt aaacgaatga gaaccctatc atgaaggcac gagaagaaaa agacggcctg
     5701 gtcatcaacg gtgtgatccg tatcagcggg tattccagca tcctgttcgt ggtgctcatt
     5761 cttttcttct tgctgcgtga aggtttaccg gcattaacca atatcccgct ccgctctctc
     5821 ttcagcattc gttggtaccc gattgaaggt tactacggaa tcctgccgtt aatcttcggc
     5881 agcctgctga tcaccctcgc ctccaccctt atcgcgttgc cctttggcat tggcaccgca
     5941 gtatatctct cagaagtcgc tccgccctgg ctgcgtgacc ttttaaagcc catcgttgaa
     6001 atccttgccg gcatcccctc ggtggtgctc gggttcatcg ggatcttggt ttttgcgcct
     6061 ttgtttcgaa aaatgctgaa tattcccacc gggctgaccg cgttcacagg tgccattttg
     6121 ctggcattta tttccattcc gaccatcgtc tctgtggccg aagacgcgct caacactgtg
     6181 ccccattcct accgccaggc atccctggcg ctcggagcca cgcagtggca aaccatctgg
     6241 gatgtgaccc tgcccgcggc gaaaaccggc gtgttaaccg ctgtgatgct gggtgtcggg
     6301 cgctcgctgg gtgagaccat ggcggtgatg atggtcaccg gtaacgctcc gctcatgccc
     6361 ttgcatttaa actcgctttt caggccggtg cgcaccatga ccgccaccat cgcttcagaa
     6421 atgggtgaag ttgcctcagg cagccttcat taccaggtgt tgttctttat cggtatcact
     6481 ttgttccttc tctcgctcgt tatcaatatc accgccagcc tggtaagcac caaagcccgt
     6541 caacgctctg agaggttgtt atcctgatga aagcagccca cctcacccgc gcgaagatgg
     6601 cgcaaaccat cgggttccac ctgctgcggc tggtcacact catcaccatc attcccatca
     6661 tcggactggt ggtttatata gtggtcaagg gtgcgccagc catcagctgg gaattcctca
     6721 cagctgcgcc gtcgaacggt atgcgggaag gagggatttt tcccgccatc gtgggtacgc
     6781 tttatctcac gctgggtact gcgctctttt cagtgcccat cggtgtgctg gcaggcatct
     6841 acctggcaga atacgcccag gagacagcgt tcacccgcat catccgcatc gccatcatca
     6901 acctggcggg catcccatcc gtggtttacg ggctcttcgg gttgggatta ttcgtgttgt
     6961 tccttcattt gggcaccagc atcctggctg cgtcattgac cctttccatc atgacactgc
     7021 cggtcatcat cagcaccact gaaggagcgc tgcgcagcgt gcccaactca ttccgggtgg
     7081 tctccacctc gctgggcgca acgcgctggc agaccatccg ccgtattacg ctgccgcaag
     7141 ccttgcccgg gatcatcacc ggcatcattc tggggctgga acgcgctgcc ggtgaaaccg
     7201 cgccaattct tttcactggc gccaccttct tcttaccgca actaccgcgc tcgccgttag
     7261 acgccaccat ggcgctgccg tatcacattt tcgtaatctc cacccagatt cccggcatgc
     7321 ccatccagat ccaatacgga accgtgctgg tgttgctggt gttcgtgctg ggaatgaacc
     7381 tcattgccac cttgatccgt tctcgcgcgc gggcaaaacg ccaatggtga cccgatgaaa
     7441 gcgttacagg tacaaaacct ttcagtgcga tattcagata ataatgagag cctgaagaac
     7501 gtttcgatgg aaatcgaatc acgggccatc accgtgctct tcggtcctgc tggcggcggt
     7561 aaatccacct tcctgcgcac cttgaaccgt ctgaatgacc tggcggatgt gaaggagata
     7621 aaaggccggg tgctttttca ccagcgcaac atccttgacc ctgaggagga tgtcatcacc
     7681 ttgcggcgaa aaatcggtat ggttttttct cgcccggttc ccctgccttt gtccatcttt
     7741 gataacgtgg cgtatgggct ggtgatggct ggcgaacaca atcaagccag gctgggcgaa
     7801 aaagtagaaa aagccttgaa acaggcattc ctgtggaacg aagtgcacga ccgcctgaac
     7861 caatccgcct tcagcctttc cggcggtcaa cagcagcgga tgtgcctggc acgtgtgctc
     7921 gcgcttgaac ctgaagtcat cctgctggac gaaccaactt ccgcgcttga tccgatcgtc
     7981 acctcatcca tagaagacat gctgcgggaa ttaaaaaagg agtacaccat cctcatcgcc
     8041 ccgcacagcg tgcagcaggc cgcgcgcctg gccgattacg ctgccttctt cttgatgggt
     8101 gagctgattg aatatggcag cggcatcgaa atcttccagg tgcccaaaga tcagcgcacg
     8161 tccgattata ttaccggcag gtttggataa aacacaacga cccttctaaa agaagggtcg
     8221 ctgagtgtgt aagcagggtt actttctctc tttgatcagc ttctgcgcca tcaacgcgcc
     8281 gtcacggatg ggttcgtacc cggtgcagcg gcaaaggtgc ttgctgagcg cgttctcgat
     8341 ttcactctga gcggcatctg gtttttcttc caacaacccg tacagttcca ttacaatgcc
     8401 gggcgtgcaa aagccgcatt gaatggcgcc ggattcggaa attgccttct gaataggatg
     8461 caactcagtg cccttggcca acccttccac ggtgagcacg tccgcgccag cgagcttctt
     8521 gatcggcata ttgcagcttt tggtagcctt gccattcacc acaatggtgc aggtgccgca
     8581 aaccgaatta tcgcaaccac gcttggtgcc ggtcaagttc aacacatctc ggatcacatc
     8641 aatgaccagc atctcagggt caacttcaac cttggtcaat ttcccattaa tagtaaaagt
     8701 gattttttct gccattattt cacctcctgc ctggcttcca gggctttttt catcacatcc
     8761 ggtgtgatgg gaatctcaaa gaaatcaata cccaccgcgt cacggatggc gttcatgata
     8821 gccggcgccg gtccaatgac cgagtgttcg ccaaccccgc gcgcgccaaa cgggccaacg
     8881 gcatcgggcg tttcgatgaa ttcgatatcc atcgggatgg cttctttgta agtgggaata
     8941 tggtacttga acaactgcgg gttgttgatc ttaccgtctt cgctgatgtc cagtttttcg
     9001 tataaggccg cccctaccgc catggtcacc ccgcccatga ccgaaccgcg gatctgcttg
     9061 gggttgatga tttgaccaac gtcgaacacc gaggtgaact tatccacaat gatcttgcca
     9121 gttttcttct caatacggat ttccgctgcc gtagcgccga aggtgtaatt cacacccagg
     9181 ctgccctgcc cgttttcgtc tgggttggcg tagcgcggca ggcgcgcgtc agaaacggcg
     9241 tgcgcatatt cgccaatggt gatgccatct gaagtaatat agccgcggct taggtctgcc
     9301 accgccacac ggatatccgg gtcattgcgc aggtaaacgt aatcaccatc atattccagg
     9361 taatccacat ctgtcttgag tacctgtgcc gcagtgttct tcaacaccgc gatcagcttg
     9421 tccgccgcgc gcacgatagc gcgcccaccc tgggtggtga acattgagcc aatggtctgc
     9481 cattcatacg gtgaatactg cgtatcgatc tcggtgtaca ccctcacccg ctccggggcg
     9541 atcttcaatg cttcggctgt cacctgttga acgacagtgc gcagcccctg ccctacttca
     9601 gcgccgccca tgctcacact gatcgaacca tcgatattca tcttcatata acagcttttt
     9661 gtggaaaacg gcgcgccctt ggggctcttc atcaccgccg cgaacccgcg gccgtagtaa
     9721 taattttcat cttcggctgg tttcggtttg gagaagacct tttctttgac catgttggaa
     9781 caggtcatca ggtctccgtg gctcttccac atcttttcgc ccagcgcatt taccttgcct
     9841 tcgacaagat agttcatctc tcgcagcttg atcgggtcca tgttcagctt gcgcgccagc
     9901 aggtccatca tgcgctcggt ggcaatctgg gcttcctggt gaccataacc acggtaagcg
     9961 ccaacaggcg gggtattggt gtaaaccgtg taacccgtca ggtcgcagtg ctcgaattcg
    10021 tatggtccgg ctgagttgtg cgtggcagcg atagtgatat tgaccgccgt atccgcgtac
    10081 gcgccgcccg tgtgcagcac ctcgttttgg atggccacaa gcttgccatc tttacgcgcg
    10141 ccaatcttca tgcgcgaacg aataccatga gccacgagcg tggaggtgaa atcttccttg
    10201 cgtgaagcca cccatttaac cggatgacct ggcacaaagc tggcaatcca ggcgacggtt
    10261 tgctcaacag tgatatcaga tttatatcca aaacagccgc caatatcggg gatgcgcacg
    10321 cgcacatctg aaactggaac accataagag tgcgccagat catcacgaat gatgaagggg
    10381 caaatggagg aagaccagac ttcaatggtc ttatcctctt tgaaccacac aatcgaacca
    10441 tgcggttcga tagccgcgca tgagcccagc gggtaattga attcaccctc gacaatgaca
    10501 tcggcgtctt tgaaaccatc gacccctttg cctttcttaa ggtgataggt atgggcgatg
    10561 ttggtgccag gtgtgggctt tagcgtgggc aggtgccagt actcgccgtt ttcttcatga
    10621 acaagcgctg cgccgggttt catcgcgtca cgcgcatcaa tatacactgg caaaggtttg
    10681 tactccacct tgatcttttc caacgccgcc tcagcctgcg ccaacgtttc agcgatcacc
    10741 gccgctaccg gctcaccgat gtagcgcacc ttcttcaccg ccataggcac caggtctttg
    10801 aaattatccc catagtgata gtggcagttt tctcctgtga ccactttcac cacacccggc
    10861 atcttttccg cttcagtctt atcaatacgg acaatttcgg cgtgtgcgta ctcagggcgc
    10921 aggatggccg cgtgcagcat gcccgggatg cgaatatcat ccaggtaaac cgctttgccg
    10981 gtcaccttgc tgatcgcgtc cggtcggttg taactctttc ccaggtattt aaatttcatt
    11041 ccttgctcct ttcacaataa ataatcagtc gggtggtttg ggaaataacc acctctgcgg
    11101 gtctttgact ccttaatggt aaccaattat gcatcaggaa tcaaggcgaa taggatataa
    11161 gtcatgaatt tcaggaaact tcgcttaatc aatcctgtcc accaatcaaa acagtggggc
    11221 accagtctga catggaagaa ataagacgtt ttttctgtat ttattgagcc tgcctttctg
    11281 ccatctaccg cagcgttgga aaaaatgggt ctggagaaga agggcgtggc aattaacgca
    11341 caaacaccgc cgtcagcgcc aggtctcccg tcagcgtgat ctcctgactc tgcgctgttg
    11401 agtctccccc cttcaacccc tcccagtgag agaaacgata gccttcagcc ggcacagcgg
    11461 tgatcgtaaa cggcaccccc tggaaataga ctcccgccca gttagaaggg tcttcaattc
    11521 caaccatgcc ttctcggatt tccagcctgt tgaccgtgac gtaccccttg cccgggtctg
    11581 ttctgattct cagttccgcg ctccccgcca aatcaaaata atccgctatc tgctgccgga
    11641 gttcttcggg tcgcgccctg gcgaattctc tgatctcctc cacgtttgcc cgccagcccg
    11701 ccagcgatcc ccctggcatc ccccaccgca aaatatgctc ttccatctcc ggttcatata
    11761 cccgctgcac ttcatcgatc gtcgccaaca caacctctgt cttgaaattc gtgttcaaca
    11821 ggtctgcaag ccgattgatg aatgaccttc tgaactcctc gttttgcagc aacgaacgga
    11881 acaaataagt tccctgagaa ccttctgcgg tcacgttctc cattgagttg aagtatcggt
    11941 ctttaaagcc cgagtccata tcgatgacca tccagcgcca tttcccgtcg tgcccataca
    12001 tctcgccgct ctcgggtaat gcgcaatcta cttttttcct ccagtaccaa atattgtttc
    12061 caagccaatc cactttgttg ggataaattt gtgatatgtt atagtcaatg tagttctcca
    12121 tatccatcag ctcgctcatc gccgcgtaag cctgcgcgtc agcgagcgtg ctgacggtgc
    12181 gaaagccgtt cgtgtcataa tgctcgtcta tcagcctgag catatccgtg tacaggttct
    12241 catcacccgg gtcccccaga tataattctg attcactgcc tacaagcacc accacatccc
    12301 caggcggtat gtgataataa ttctggaaat atcccgcgtc ataccggctg cggaggttat
    12361 gaatgcccca gtattcacca ttcagaaaca cgatgaccgg gtagttcccc tgtatatcca
    12421 gctgaaggtt ctccagcagc gactgtccga tgatgtccgc gatcatggta tcttccatgt
    12481 ctcccgctcc cccggtgcgc aggatcagcg tctcaaactt atccacttcc agccgattta
    12541 aacgctcgtt caagccagga aagaaatcaa actgcagggt gctttcaccg ctgtactctt
    12601 cacgggcgta caacctcaat gatttttgcg ggtaccctcg cgaggcgcgg ccgtgaatcc
    12661 tgataccaat ctcttgcgca aagaccacct ctcctgacgg atcgaacagt tcaacgaatg
    12721 ccggcctttc ccatttcaaa cccctctgcg tgtagttcgc ggtattatgc aattgatttg
    12781 acgggtggtc attttgataa agctcgcccg gaacgtagat cccgtaccgg tcgtcaaaca
    12841 acgtcaccgg cgcgaccgtc aggctgagca ccggcagcga gaaccgctct ttcaccccct
    12901 cctccacgaa gtatgtgtgc gtgaccaccc cgctccttcg ctggtcttcg cctactacca
    12961 ccgcccgcac caccatcgct ttgaacatgt tttccggtgg tttgtagtcg tctattctac
    13021 tcgtttcaat ttgcgacaga cggttgcgcc ccgcgctctt gtcatttacc gttatcggcc
    13081 ccgtgtattc ccgcgtactc cccattgcca tccgtttttc atcgatcgcc ggttccgacc
    13141 catcggtcga gtaaaatatc ttcccgcctg cttctggcgt tgtcagtacc agcgcaaacc
    13201 cctcctcgta aaatccactc tcatgtgaaa attttggacc cgccacttca ctcgcgtaat
    13261 tgggtaagta gtaaggatac cgatagctca ccttttccca ataccccctc agccccaaga
    13321 acgggtcccc ttttccttcc cctatttcat tttccagtat gttcaccgtg acccttttac
    13381 cagcgctcac atcatcctgc aagaccccat ggctcatcat ttctaccagt acctcaatat
    13441 tccgtatgga tgtgtgccga atgttcaaat acgtcagcct gttcaggtta gtcaatatcg
    13501 aaacctggtc tcctatgggc acatttctca aaatgagact gttgagcatg gtcaggttct
    13561 tgagcggcgt gaggtcctgg atatcctcgt tgctgtgcaa atttaggtag gtgagctgct
    13621 tcatttcagc cagcgcagtg atgtctctta cccggttttc tctcaggttc agtttctgca
    13681 acttcttcaa tccttgcagc ggagtgaggt tctcgatttg attatcactc aaatccagca
    13741 cccgcaggtt cctgatcccc tcgatccctt ccagctcccg aattcctcta ccgctcagat
    13801 ccaactcgat cagcgtcaag agttgatgcg tgtatagccg cccttccggc ttccctaaag
    13861 cttccctcac cccctcctca agtttcggat cgctgaacac aatttcgcgt tgtgcctgct
    13921 ggttggcaaa caacaacacc aacgaaacaa gcaccaaacc caccacatca gccagccccc
    13981 gcgcccgcag acgccgttta accactacct ggtataccag caaccaaaac atcaccgcgg
    14041 acaaaaatac cgtgaccgta tctgtcagca gtcccgccat cagcaacacc cgccgtaccc
    14101 ggtccgcgtt cttccacgtg ttcgggatcg ttaccgtttt ctcaacctcc ccctcctccg
    14161 cctcagccag ctcaacgctc tgcccctccc cattgaccag gatatccacc gccccttctc
    14221 ccggcccggt ctgaaaacgt atcctgacct ccccctgaat attgctcgtg tagcgcaccg
    14281 agccgctgtc cccctgcccg ggggagaacc tcagtttccc ttccatcacc tgccaaccac
    14341 ccccgtagct cagatttcta agattgattg gggtaggaat gttcttcggc gggtcctgcc
    14401 tgaccatcga caggatggtg atcccctttc ccgccactgc cagctcctct cctttcgccc
    14461 tgatctccag ctccattcgc tttggaaagg ccggaaggct gtagaacacc gccatgatga
    14521 ccaccgctgt caccaggctt agccccccga ttattttcag cctctctttg cttatcccct
    14581 ccaataacct gttcaccagc ggaagtataa acagcagccc cgcaaacacg atccataacc
    14641 aatgaacacc cagcgttatc gccttttccc cacgcgacac cagtaagaaa tgggagaaac
    14701 gcaccaccag gctataggca aagcacaagc ccgctatcaa tgtggcacta acaggaacga
    14761 ggtttttgct cttcaaaatg ctcatccgct ctctgccgct tcttttcctt ttcccttacg
    14821 cgctccaccc tggcgtgacc agcgcggggt acgccaccat catcccctgc acgtacttgc
    14881 tgttcgccat gatcatcaac ccgttctgct gcaccaacct gcgcaaccac cccggctgct
    14941 gtttgatgca ctttatctcc aatacgatca gccccggatg gtgctcgtga aaatgcggtg
    15001 cttcaggaaa caacgttcgg ctgtgcgcgc tcttcacccg gtgatcaaag gtgatgcgta
    15061 tctcctcccg cgccctcgat cgatatcctt cccgtctgta ttcggtcagc agcagcgggt
    15121 tcaatccctt cagatgaaca tatcgctcaa attcttccat cacctggtta cgtattttcg
    15181 gccagcgctt ctgccctatg aacatctcgt attccgctcc gctgatgaac gcgccatatt
    15241 tctccgttat ggcgtttttt cgcactttca tctccgcccg gatggtcgtc ccctggctaa
    15301 gctccctgca atacgtgcgg aggcgaaact tgatgcggtc actgttgcca tctatctttt
    15361 cattaaacgc gtctaatcca agcgagtcat aatacacact gcacaccagg tacttcttct
    15421 ccgccgcgcg ctccgtgtac tcatctatct ccatgtacgc ccgaatctga ttcctcaccc
    15481 gcaaatactg ctctacggac agccgatact tgtactcgaa ccgccagttc ttttcatcca
    15541 accgcgctga tggcttcatt cccgttttct ctcaccctgc cctttacaga ggaagcgcca
    15601 gctgcggagc cagcaccgac accttgttca ctccgctcat ctccttcatc tctgcgacca
    15661 tcccttctac cggctgcttc tccctcttcg tgaacctcag ctcgtagatt acttcccact
    15721 tctctccatc tacatcaaac gacctcgccc gctttccagt cgtatatttc tccagcacgt
    15781 tttcgatctc ttctttcctg tattctccac tcccgttcac caccagtaca aaatcagtgt
    15841 tcggcgaatg tccatacctg aaaaagtaca gcgccgcaac gatcaccgcc acgatcgggg
    15901 tcgttacccc aaccaccgcc cagttgtagg tccccgcccc caaccccacc gcgatcaccc
    15961 aaaataaaaa taccatgtcc cgcgtgtctt tgatcggcgt cctgaaccga atgatcgaca
    16021 ggctgcctat cagcccaagc gacagcacca aatcgccacg tatatacaac atcacgatcg
    16081 acacgatcgg cgccagcaat accagcgtcg ccataaacgt gtgctcatag ttcaaccctc
    16141 gatgtgtcca tttgtatatc cgcgatatcg ccagccccat taccgccgcc aacaacaccg
    16201 agaacaatac atcccctatg tcttgaccag atatcaaaat ccgatctaat agccctgcca
    16261 acctgtcgat gatcgtcatc ccttttttcc tccttnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnncgctt cgtttttcac tctttatgcc acccgcaaat cgaatcgcta
    16441 cccaaccatt ggttgaaatt acgcgtggaa gaaaagatta aggaatcggg gctcaacttt
    16501 accatcttcc agccaacgcc atatatgcaa aatgtactgg ggcagcggca gaacatcctt
    16561 gaaagaggcg tgtatgaaat tccgtatcag ccttccactc tgctgggcat ggtggatctg
    16621 gacgatgtcg ctgaggtggc tgcgcgggtg cttctcaccc cgaaaaccta cgactgggca
    16681 acttacgagc tcgccgggcc agaaatcctt tctcaaactg aagttttgca tatcatccgg
    16741 ctcgtcaccg gtaaggagat tgctctgaag gtgatttcac gcgaagattg gtgcgcgcgg
    16801 gcggcaagca cgggcatgcc gcaatacgcc gtcagcattt tttatcgcat gttcgaattc
    16861 atggaatcca gcggtttttg gggcaacccc ggagttttgg aatggctgct ggagcggaaa
    16921 gcgcgccgct ttgaagcctg gttccgcgct gtgggtaact ggtcgtaatt tcgcctgtgc
    16981 ttttctccag gagtgacctc gaaaaagtta tatatattca cggtccatcc ccacgcgtgt
    17041 ggggaatacg gtgtcgccgc cagtcaactg tttgatcagg gcggtccatc cccacgcgtg
    17101 tggggaatac acctatgtac tgcatgatgc cattcttctt agcggtccat ccccacgcgt
    17161 gtggggaata cgaggaagga ggcgtgcaca ccaacgttgc gtccggtcca tccccacgcg
    17221 tgtggggaat actggttgtt tggatcaggc atcgcgccgg cctccggtcc atccccacgc
    17281 gtgtggggaa tacgtctccg ctttcgtatt ctctaagacg accccggtcc atccccacgc
    17341 ctgtggggaa tacggttgga ccagcccagt ccttatctct taccgcggtc catccccacg
    17401 cgtgtgggga atacggagga tggagcatgc cagataaaac gaaaaatggt ccatccccac
    17461 gcgtgtgggg aatactgggg attggcggtc gcacttgctt tacgctgcgg tccatcccca
    17521 cgcgtgtggg gaatatttca ctgtatctgt atacctgtac gaccataacg gtccatcccc
    17581 acgcgtgtgg cgaatacctt gtaaagcagc ctggctgtgc ggtccagctc ggtccatccc
    17641 cacgcgtgtg gggaatacgg catcaacaag tggttctttg actcgagcag cggtccatcc
    17701 ccacgcgtgt ggggaatacg acgggtggcg tgaaatggcg cgcgacatta ccggtccatc
    17761 cccacgcgtg tggggaatac gtcatgtgat ctcctactca agcatggggc tgcggtccat
    17821 ccccacgcgt gtggggaata ctttgttctc accacactgc gccccttatg ttccggtcca
    17881 tccccacgcg tgtggggaat acggcatgac gggcgggata agaggttaat atggcggtcc
    17941 atccccacgc gtgtggggaa tactacgtgt tctacggcgg gcgcgggtca accaagggtc
    18001 catccccacg cgtgtgggga atacgccgct ccggccacag aaacacccac agccaacggt
    18061 ccattcccac gcgtgtgggg aatacgtcct gaacatcctg gtaagccaat cggtaaccgg
    18121 tccatcccca cgcgtgtggg gaatactttt tccagcgtgc gcgtcgcctg cgccacctcg
    18181 gtccatcccc acgcgtgtgg ggaatactac actatggtta tgcaggagtt accagcagtc
    18241 ggtccatccc cacgcgtgtg gggaatacct tctccgtgta cgccagcgct atccacctgt
    18301 cggtccatcc ccacgcgtgt ggggaatacg cacatatgca actatcgcag acacacaaaa
    18361 atcggtccat ccccacgcgt gtggggaata cgatggagtt caaaatatca tctgtgctga
    18421 tgccggtcca tccccacgcg tgtggggaat actttgcccg gatgttggac aggttgtacg
    18481 actgcggtcc atccccacgc gtgtggggaa tacgctgatg gccactacgc tggcattcgg
    18541 gatggtggtc catccccacg cgtgtgggga atacgcgaga tgcaagcgga caaggcatac
    18601 gaggcgcggt ccatccccac gcgtgtgggg aatacgcaac ggacgagcgg aaccaaaacg
    18661 aaatgtacgg tccatcccca cgcgtgtggg gaatacgtta gtgcggtagc tgcgcgtgtt
    18721 cgctaccttg gtccatcccc acgcgtgtgg ggaatacctc accgctttcg ctgatgtcca
    18781 ctacgctggc ggtccatccc cacgcgtgtg gggaatacga atgttaatgc caacttccaa
    18841 cagaactgga tggtccatcc ccacgcgtgt ggggaatact cctgtctcct ttttgaatat
    18901 aacctggccg tcggtccatc cccacgcgtg tggggaatac
//
LOCUS       DAOSPM010000033        18373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_3041_length_18373_cov_13.408232, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000033 DAOSPM010000000
VERSION     DAOSPM010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18373
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3041_length_18373_cov_13.408232"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..512
                     /locus_tag="PLW25_04470"
     CDS             264..512
                     /locus_tag="PLW25_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53776.1"
                     /translation="MLNGQLFLKRFAWQPNAEYPDFNVNFEAYTDRDILELETLAPLT
                     ELQPGQSVSHSEEWLLFRSIPSPSSEEDVDRYILPLLS"
     gene            complement(557..1594)
                     /locus_tag="PLW25_04475"
     CDS             complement(557..1594)
                     /locus_tag="PLW25_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOS53777.1"
                     /translation="MSLIDEIHEQPQAIRKLLNSQGEAIATIAGQVRAHGIEYIYLAA
                     RGTSKNAGRYAQYLIGAVNRLPIALAPPSLFSIYHQPPLLKNALVIGVSQSGRSPDII
                     SVIEEARRQGVVSLAVTNNPVSPMADAAEFILDISCGPEVAVAATKTYTNSLAALALL
                     SAELAENAEMKRALENVPAWMEEMLGREDEIAQQAASFCSMNHCVVLGRGYNYATVNE
                     WSLKMKELSGVMAEPYSSADFLHGPIAMVKGDFPVLNVAPSCIAYPSLYELLGRLREE
                     RRAEIYSITDRDEVLQLSRAGQRLPQGIPEWLTPLIAIIPGQLFAYHLTRHKGLDPDN
                     PSGLSKVTETH"
     gene            complement(1605..2423)
                     /locus_tag="PLW25_04480"
     CDS             complement(1605..2423)
                     /locus_tag="PLW25_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS53778.1"
                     /translation="MRIALHGSSFVASQFGYTPPGSWEECVNALSAFYSPPVTFEARF
                     QGLVARVRELGFFRMDVWQPAELNWQWAGPEHIAAAVRALQHEGVSPTSLAGEFGETR
                     AEFESACALSAGIGAPLLSGTTALYHTDRAFVIETLKRHGLRLAIENEVELTAGEMLE
                     IIGDGGNGVIGTALDTGWYATHGYDPVQAIDELRQFIFHVHLKDVLPGDAHINCAYGK
                     GIVPIEACVKGLLKIGYDGVISIENHTLDHDPTGELVQAREWLESLIKSNTPLN"
     gene            complement(2425..3561)
                     /locus_tag="PLW25_04485"
     CDS             complement(2425..3561)
                     /locus_tag="PLW25_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS53779.1"
                     /translation="MGKGRLRIGVIGCGGIAQMMHLPFLQKNADRFEIAALCDLSNKV
                     LSALARRYHLPDTGCFTDYQLMLKQNLDAVLVTSGGDHTPQVRAALLAGAHVFVEKPL
                     CYTQAQALELGRLAQEQKLRLMVGYMKRFDPAYQRARQLLAGLGELRYVQLNTLHPEE
                     EGYLYFQGLFQAEDIAPETIRHLRAQEDQAITAAVGEIPAYLRAEYTDVLLGSMVHDI
                     NVLRGLLGEPQKVLFAEHWPQGEKPGGITTTMMFSPDLRVVYTWVFLAELRDYSQEIA
                     LMSASQRLRLQFPSPYLRHFPTSLTHQYMKDGAAVEEKITVNYDEAFEQELLAFYDCV
                     VNDREPLTGADDARADIAVLQQILAGLHPVGLSGEAAGYMLGRN"
     assembly_gap    3692..3791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3936..5279)
                     /locus_tag="PLW25_04490"
     CDS             complement(3936..5279)
                     /locus_tag="PLW25_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HOS53780.1"
                     /translation="MNQKEKNLVNEAAKHALEILHQCVTPYGFRASADPVGYPQVWSR
                     DSSVTLLGALASEDKALINAGRASLDALSGAQSCWGMIPLNLNPESGDISTENAGSCD
                     ANLWYILGQYALYRATGDKDALARSWPRITSALTWLQYQDMNECGLLEIPEAGNWMDL
                     IAVRYNTLYDNTLFYAASLAYEEMLRDNPAELAPLPCHISADSIHERINLLFWIDRCW
                     VASHFAEHLEKLKAIRLEWFMLYYNIGTISSRPFYLPWVAFREYGDWCDSLANLLAIL
                     TGIADGHRAQHILRYMQQVGMAEPYPTKAIHPPIYPGETSWREYYRSRNLNLPDQYHN
                     GGIWPMVGGFHIAALTRCGWQAEADKLLVQLALANQQSSGAPWEFNEWMHGRTGHPMG
                     YAHQAWSAAMYLYAWKAVNGAELPLFDDLLSAKPARAVAEEVNTPYFHPGGGQVG"
     gene            complement(5303..6133)
                     /locus_tag="PLW25_04495"
     CDS             complement(5303..6133)
                     /locus_tag="PLW25_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOS53781.1"
                     /translation="MKRQAWQRLLPNYLILSAFLLLILLPIIGLVFSAFKTDIEVIKG
                     PLTLPSQLRFASFKEAWTTGRFNFFFRNSVIVTVVVVVISVFLSTLTGYAFGMLPVPG
                     KKVLYPLLLVGYMVPFEAVVIPLYHMMDNLKLTDTYWALILPQIGLSVSFGTLWMSSF
                     FSTAPSELVDAAAIDGCNRWQTLWRILWPLARPAVLTLVVLIFMWTWNEFLLALVMIQ
                     KETMRTLPVGLAFFQGRYSANIPLMAAGALIVAGPILLVYIVFQRYFIRGMLGGAVKG
                     "
     gene            complement(6130..7029)
                     /locus_tag="PLW25_04500"
     CDS             complement(6130..7029)
                     /locus_tag="PLW25_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOS53782.1"
                     /translation="MKSQRRWPGESRFHGWLYLFPGLLIYLVFVFYPIIETFRTSFYK
                     WDGFSRERVFVDFQNYISLVTDAQFLKALLNNLVFIIFYSIIPILLGLLLASLLGRKP
                     LPGMTFFRTGLFLPQILSMVVVGVTWRWIFNPNFGLLNVGLRAIGLGAFTRAWLGDFQ
                     LALPSVGAVGTWVQYGFCMVLFLAGMQRIPNDQYEAAELDGANEFRQLLHITLPSLRA
                     ELGVALITTIIAALRVFDLVYVTTRGGPGDSTLVTGFLVYRSAFQQNQLGYASTVATV
                     MTLLIFGISLIILRFQSRDGEGV"
     gene            complement(7099..8475)
                     /locus_tag="PLW25_04505"
     CDS             complement(7099..8475)
                     /locus_tag="PLW25_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOS53783.1"
                     /translation="MKRITFILSVVMAFALLLSACAKPAPTEAPSVPEVPAATEAPVV
                     TEAPAAPEEVTLVVWDQFYRDVESEVMETLNAEFEAAHPGVKIERVVKTLDDLKVTLK
                     LALAQEDGPDVAQVNQGRSDMGAMVQADLLLPLNDYAAAYKWDERFSSSVASRNSFTP
                     DGVTFGQGNLYGVSPTAEVVGVFYNKTIMDANGWTLPTTFDEFQTLLAGIKDAGMTPI
                     SFGSLDGWNAIHEFSAVQHLLVSLDYINDFVYGVNNVTFDTPENQEAARVMQDWVDKG
                     YFSPDFAGIGYDDSNKLFKAGVGGVLTITGSWLAGELVGDTDQEFGFFLLPANPGHTR
                     LAIGGVGIPFSIRKTTTKADLAAEYLDWMTSPRAAELWANAGMLPAMPLPEGYVAEGN
                     PLFNDTLIAWDTINSENAVGHYIDWATPTFYDTIVAELQKLFGKVITPAEFTKAVEVD
                     YAAFLAGN"
     gene            8654..9628
                     /locus_tag="PLW25_04510"
     CDS             8654..9628
                     /locus_tag="PLW25_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS53784.1"
                     /translation="MVVLSTAKEEGMTLTWDVFGIGTAAVDDLLLVERYPEADSKVNI
                     LESMRQGGGQTATAMVAAARQGARAAFCCRLGLDDLSRFTLSELQKEGVETSACHQDR
                     QGSPYHSIIIVDTQKHTRTIFHSGGNVSPPPELINAELISRTRVLFVDDNSGPAGIKA
                     ARIAASLGIPVIADVEPDPPPEVVELLPLVDHLIIGSKLAQTLSGESSEPAMAFALCG
                     TQRACCVVTAGEKGCWYAQRGASAVHVPGHKVDVVDTTGCGDVFHGVYAAALARGDSL
                     PDAVALANASAALKATRPGGRLGIPDLETARAFLHGSHTQTLEGLTHA"
     gene            9668..10708
                     /locus_tag="PLW25_04515"
     CDS             9668..10708
                     /locus_tag="PLW25_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOS53785.1"
                     /translation="MREIWQVVQQWQAEKQQVALATVVQVTGSSLRPEGSKLVISSGG
                     AIHGSVTGGCVEGAVFEEAREVLKDGAPRLLSYGVTNQQAWDVGLSCGGSVQILVESL
                     DTPDWQAVLPPLARILAENQLAALLTVIHGDSVGKKMLVLPDGETHGSLGNRELDQEA
                     IGNLPENWATRLPLQITLKNGEVLFADFIVPPPRLVIIGASHIAIPLVALANTLQFHT
                     IVVDARSAFATRERFPHAHELVVGWPADVLQQLKLDAATCVVALSHDDKLDNPALLFA
                     ITSPARYIGALGSRTTNAKRLEDLRAEGAKEQDLKRIHAPVGLKIGARQPEEIALSIM
                     AEIVATAHGHDG"
     gene            10698..11333
                     /locus_tag="PLW25_04520"
     CDS             10698..11333
                     /locus_tag="PLW25_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOS53786.1"
                     /translation="MTDSTGRHDHKKVAAILLAAGGSTRMRQPKLLLHWHGQPLVRWT
                     ARLALFTGCEPVLVVTGANAQEVQTALAGLPLIFTRNPDWQKGQSTSVKAGVKALPAG
                     IDAALVFLGDQPHIPPRVPRELLRVFQQDDPAEAILIPTFQGKRANPVLLEKRLFEAL
                     ARLSGDAGARALFSQFPVKQVPFEEPDLAFDVDSPEDYQKLLARPAPLFKD"
     gene            complement(11403..11651)
                     /locus_tag="PLW25_04525"
     CDS             complement(11403..11651)
                     /locus_tag="PLW25_04525"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOS53787.1"
                     /translation="MNILIWILVGAIAGFLADWVVKGIRMGLLGKIIVGILGGFVGGW
                     LFSLLGINLGNGILGAVISAFVGAVILLMLLLIIRRRR"
     gene            complement(11780..13372)
                     /locus_tag="PLW25_04530"
     CDS             complement(11780..13372)
                     /locus_tag="PLW25_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOS53788.1"
                     /translation="MTLAGFPIPLPPQLSFLAKPLPVFLVNIAAWMVLAAFIYWVAMP
                     LIRRFTLALPGEVEDIVLGIIRRPLALLVLVIGIRVSIRYLQAAEDLQRLLIRLSMTV
                     IVALVGHIAGRLIRDVLVYYGESWARRTESQVDDILLPVLSLFGPLLLFTIGALIVLP
                     MWGVDVSSVLLGAGVLGLVLGLALQETLSNIFSGLSLLIEAPFKRGDLIRLVSGKVCE
                     VQRLGLRSTTLLSLDEQATIYMPNKVLAADYLVNLTKPTAEQRCSVSVSVEASNDLTL
                     VKDTLYRIAAGHPGVVASNLAKKTASILSQAEKIRAWAGGLSAEDPTRFELEKMAADN
                     ESAIERLAREGRVNDQLVLLKEEIRNMIRSIRSRETSGLSRAEVAEILNEHVNPVDNT
                     VRTLAEQCRDWSTTPDRWLNDHDYWQQRRLWDSRNEELLRHWNNLKNCITNPDERVEL
                     RLDDLSAVLIDWLDKEYKIVPGEWKDPMVEIKNVSADSAELELHYYVDNIRLEMDGRP
                     RRIRSELNHLIKHEFVQEGIWS"
     gene            13623..14492
                     /locus_tag="PLW25_04535"
     CDS             13623..14492
                     /locus_tag="PLW25_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HOS53789.1"
                     /translation="MDFSFIVIILQLIFLEGILSIDNAAVLGAMVTPLSDHEPIPWPK
                     ALAGFGHAMNKILGPQRTAALKVGLIGAYAGRGIMLVAASYVIQNPWLKLVGAAYLIK
                     LALDDLSAHGASGSDEDGDRPVKERTFWATVLAVELMDLAFSLDNVVAAVSLSKKLWV
                     VMLGVAIGIVMMRFAASLFSKVVEKAPVLKTAAYILVLNIGVELLIEDLGHIEIGDWT
                     RFGISIATILLALAYERFKFMKFFRPLLIWLSQGFALINGIITWIFAPIKGIFTLVSK
                     LFLRQRATPSVAE"
     gene            complement(14556..14993)
                     /locus_tag="PLW25_04540"
     CDS             complement(14556..14993)
                     /locus_tag="PLW25_04540"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS53790.1"
                     /translation="MIQITTPKTKEDFKAYYDLRYKVLREPWGQPRGTEKDDYEPISQ
                     HFMAVDDETKEVVGCVKFSEKEPGVAWVSHLAVKTDLQRTGIGRQLLAHVEAQAREQG
                     FKKIGCQARLNTTAYFEKAGYKMHGLPTRYFGTTQVVWMEKEL"
     gene            complement(15052..15336)
                     /locus_tag="PLW25_04545"
     CDS             complement(15052..15336)
                     /locus_tag="PLW25_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOS53791.1"
                     /translation="MSQVATKKKLQIRNERVKVSAYFHEQGSVLKGDAEGYCDHFEIE
                     VVVDSDAPEKEIQKLIDLARQMCFTEKALMETVKVEVAHTINGKTQPAKK"
     gene            complement(15349..15558)
                     /locus_tag="PLW25_04550"
     CDS             complement(15349..15558)
                     /locus_tag="PLW25_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53792.1"
                     /translation="MAEKKPLGFRAIRVNAEASSLDKWRKQVIAGQPETGRAYAFISD
                     EGTYMPGGEGTAPSPLTYFVSGMAL"
     gene            complement(15574..16854)
                     /locus_tag="PLW25_04555"
     CDS             complement(15574..16854)
                     /locus_tag="PLW25_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOS53793.1"
                     /translation="MNPIDKESALSYHRLGGVPGKLVIHPSKPIENQHHLGLAYTPGV
                     AYPVLEIARDPELAYEYTTKGNLVAVISNGTAVLGLGNRGALASKPVMEGKAALFKKF
                     ADIDSIDIEINSVEPETVISVCAAIGNSFGGINLEDIRSPECFSIEEQLKTMLKIPVF
                     HDDQHGTAIILGAALINALEITSKSLTEVKVVFSGAGAAALASASHLMKMGIPREHIW
                     MCDIAGLVYRGRVEEMFAEKEVFAQGDKPASLAEVIEGADVFIGLSAANIVTPEMLRR
                     MRAQPVIFTMANPDPEISYELAQQARPDALLGTGRSDYPNQVNNLLCFPFLFRGALDV
                     RATTINDEMKVAASQALAALAHEEVPAEVLKAYGLKRLEFGKDYVLPKPLDRRVCLWE
                     APAVAQAAMRSGAARLSLDLNDYQKALAERLYHP"
     gene            complement(17015..>18373)
                     /locus_tag="PLW25_04560"
     CDS             complement(17015..>18373)
                     /locus_tag="PLW25_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS53794.1"
                     /translation="QIQAQAESEVDIYFFWGEGCPHCAKAKPVLEDLAKKYPAAHLYE
                     FEIYNSQANQELFSQFGEAYGFTPRYVPTILIGKNIWEGYSDEFSQEITGVVEQCLEK
                     ACENPGSALIAGKGYVPFGENAAAPTAVPTSAAPTPAATESPDTNTENNGATSKLPFF
                     GEVDFTKIGLFLSTALISFVDGFNPCSIWVLSMLLAITLHTGSRKKVLVIGLVFVTVT
                     AAIYALFIAGLFTVLKVVSFVGWIQVVVGLVALFFGLVNIKDYFFYKEGVSFTISDEK
                     KPGLFQRMRKVMDAGDSWWGLIGGTIVLAAGVSLVEFSCTAGFPVLWTNLVAAHQVSV
                     ITFILLLLLYLVIYQLDELGIFFVAVFTLKTSKMEEKHGRILKLIGGVLMLTLAIVML
                     VNPAVMSDIGKSLIVFVIAFGVTLLILLVHRVIFPRLGLHIGTEFKKSQDRNGKHKAH
                     RH"
BASE COUNT         4403 a         5141 c         4656 g         4073 t
ORIGIN      
        1 acgcttacat ttttatttgg cattctaacc tcacgtataa aaagatgtat gaaaattata
       61 gtatacaagc caggttttca tggttttttt tcccaacata attataattc tcagggtttt
      121 ttatgacttt tatcatccgt gagcgggggc aatgggatta aaaggcggac agtagttcaa
      181 acacagattc aaattgataa gacccatcct ccgcgacacc gcagaaattt ggcctgcaaa
      241 atccgtacgg ttgggctgcc tacgtgctca acggtcaact gttccttaag cgcttcgcct
      301 ggcagcccaa tgccgaatac ccggatttta atgtgaactt tgaagcctac acagataggg
      361 atattctgga attggaaacg ctcgcgccgc tgacagaatt gcagcctggt caaagcgtaa
      421 gccatagtga agaatggctg cttttccgtt ctattccctc gccgtcctcc gaagaggatg
      481 tggacaggta catcctccct ctgttatctt aaagagtggg cgcaccattc tctgccagac
      541 acaaatccag gtggctttaa tgggtctcgg tcaccttgct taacccggag gggttatcag
      601 gatccaaccc tttatggcgg gtgaggtggt aagcgaacaa ttgcccgggg atgatggcaa
      661 tcaacggggt cagccactca gggatgcctt gcggaaggcg ctgcccggca cgtgacagct
      721 gaagcacctc gtcccggtct gtgatggagt aaatttccgc ccggcgctct tctcgcaggc
      781 gccccagcag ctcataaagt gaggggtaag cgatgcacga aggcgcaaca ttgagtaccg
      841 ggaagtcccc cttcaccatc gcaatgggcc cgtggaggaa atcagccgat gaatagggtt
      901 cagccatcac gcctgacagt tctttcatct tgagcgacca ttcgttgacg gtggcgtaat
      961 tgtaccctcg gcccagtacc acgcagtgat tcatagagca aaaactggcc gcctgttgcg
     1021 cgatctcatc ctctctcccc agcatctctt ccatccaggc cggcacattc tccaatgcgc
     1081 gtttcatttc cgcgttttca gccagttcag cggagagcaa ggccagggcc gccagtgagt
     1141 tggtgtaggt ttttgtggct gccacagcca cttcagggcc gcaactgata tccagaatga
     1201 actcggcagc gtctgccatg ggtgaaacag ggttgttggt cacagccagc gacacgaccc
     1261 cttgccgcct ggcttcttct ataacgctga taatatcggg cgacctgcca gattgtgaca
     1321 cgccaatgac caaagcattc ttaagcagcg gcggctgatg gtagatactg aacaaagagg
     1381 gaggtgccag cgcgatcggc aaacggttga ccgcgccaat aagatattgg gcatacctgc
     1441 cggcattctt tgaagtgccc cttgcggcca ggtatatgta ttcaattccg tgggctctca
     1501 cctgccctgc aatggtcgcg atagcttccc cctgtgaatt gagtaattta cgaatcgctt
     1561 gcggttgttc gtgaatctca tcaatcaggc tcattcttcc tctttcaatt cagtggtgtg
     1621 tttgatttta taaggctttc caaccattcg cgcgcctgca ccaattctcc agtcggatcg
     1681 tggtcaagcg tgtggttttc aatgctgata acgccatcat acccgatttt caacagacct
     1741 ttcacgcacg cctcaattgg gacaatgcct ttgccataag cacagttaat atgcgcatcg
     1801 cccggcaaca catccttaag atgcacgtgg aaaatgaact ggcgcagctc atcaatcgcc
     1861 tgtaccgggt catacccgtg cgtggcgtac caaccagtat ccagcgccgt gccgatgacc
     1921 ccgttcccac cgtcaccaat tatctccagc atctcaccag cggtcaattc tacctcattc
     1981 tcaatcgcca gcctgagtcc gtgacgtttg agcgtttcaa tcacaaaggc tctatccgtg
     2041 tgataaagcg cggtggtacc gctcagcagc ggcgcgccaa tccccgcgga gagggcgcag
     2101 gccgactcaa attcggcgcg ggtttcgccg aattcacccg ccaggcttgt tggcgacacc
     2161 ccttcgtgtt gcagcgctct tacagccgct gcaatatgct ccggcccagc ccattgccaa
     2221 ttcagttccg ctggctgcca tacatccatg cgaaaaaatc ccaattcccg cacccgcgcg
     2281 acaagccctt gaaaccgtgc ttcaaatgta acaggcggag agtaaaacgc gctcagcgcg
     2341 ttgacgcact cctcccaact gccaggaggt gtgtagccaa actgactggc gacaaagctg
     2401 gaaccgtgaa gggcaatacg catctcaatt ccttcccagc atataaccgg cagcttcccc
     2461 cgatagccca accgggtgca aacctgccag tatctgctgc aaaaccgcaa tatccgcgcg
     2521 tgcatcatca gcacccgtca gaggttcacg gtcgttcacc acgcagtcat agaaagccaa
     2581 caattcctgc tcaaaggctt cgtcgtaatt cacggtgatc ttctcttcca ccgcggcacc
     2641 gtctttcatg tattgatggg ttaacgaggt tggaaaatgc ctgagataag gcgaagggaa
     2701 ctgcaagcgc aggcgctggc tggcagacat gagggcgatt tcctgagagt aatctctcaa
     2761 ttcagcgagg aatacccagg tataaaccac cctgaggtca ggtgaaaaca tcatcgtagt
     2821 ggttattccg ccaggctttt caccttgcgg ccaatgttcg gcaaagagga ccttctgcgg
     2881 ttctcccaat aaaccgcgta aaacattgat atcgtgcacc atgctgccca gcagcacatc
     2941 cgtgtactcg gcgcgcaggt aagcgggtat ctcaccgacc gcagcggtga tggcctggtc
     3001 ttcctgcgcg cgtaagtgcc tgatcgtttc cggcgcgata tcctcagcct ggaaaaggcc
     3061 ctggaaatac agatagccct cctcctcggg gtgcagtgtg ttgagctgca cgtaacgtaa
     3121 ttctcccaag ccagccagca gttgacgcgc gcgctggtag gccgggtcga aacgcttcat
     3181 atatcccacc attaacctaa gtttttgttc ttgcgctaac cgacccagtt ccagcgcctg
     3241 tgcctgtgtg tagcataaag gtttctcaac gaacacatgc gcgccggcca gcagcgctgc
     3301 gcgcacctgc ggagtatgat cgccgccgct ggttaccaaa acagcatcaa ggttctgttt
     3361 gagcatcagc tggtaatcag tgaaacatcc agtatcaggc aggtggtaac gccttgccag
     3421 cgctgaaagc accttgtttg acaggtcaca aagcgcagca atttcaaagc ggtcagcgtt
     3481 cttttgtaga aatggcagat gcatcatctg cgcgatgccg ccgcaaccga tcacgcctat
     3541 tcttaatcta cctttaccca tccggttcct cgaataacgc cctaaaagcg ggactacgat
     3601 tcatattgta tttcaatgtg cttattcacg tgaacatcca atacccctgt cttgatcaac
     3661 accctgcccg gttcatcata ataccagccg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn ngccaccatc gcggtcatgc ctatgagaac cagccaaatc acttttttca
     3841 tcatcgctta ttcacggtaa tatccggggg tcgcgctgac tgacggcacg ctgctgaaga
     3901 tttcgcgccg caatccatgc aaaaaacccg accggctacc ccacttgccc gccgccagga
     3961 tgaaaatacg gggtgttcac ttcttctgcc accgctctgg caggtttggc ggaaagcagg
     4021 tcatcaaaca gcggtagctc cgcaccattc accgctttcc aggcgtacag gtacatggcc
     4081 gccgaccagg cctggtgggc gtatcccatt gggtgaccag tgcgcccatg catccactca
     4141 ttgaactccc agggcgcgcc agaactctgc tggttggcga gcgccagctg taccagcagc
     4201 ttgtcagcct cagcttgcca gccgcaacgc gtcagggcgg cgatatgaaa acccccgacc
     4261 atcggccaga tgccgccgtt gtgatattga tccggcaggt tgaggttacg gctgcgataa
     4321 tactcacgcc acgaggtctc tccggggtaa ataggtgggt gaattgcctt agtggggtac
     4381 ggctcagcca ttcccacttg ctgcatgtaa cgtaaaatgt gttgagcacg gtggccgtcg
     4441 gcgataccgg ttaagatagc cagcaggttc gccagactgt cgcaccagtc tccatattcg
     4501 cggaaggcta cccagggcag gtaaaatggg cggctggaaa tggtgccaat gttgtagtac
     4561 agcatgaacc actccagtcg aatggctttc agtttctcca ggtgttcggc aaagtgcgaa
     4621 gcaacccagc agcggtcaat ccagaagagc agattaatac gttcatggat ggaatcagcg
     4681 ctgatgtgac agggcagcgg agccagctcc gcagggttat cccggagcat ctcttcatac
     4741 gccagcgagg cagcgtaaaa cagcgtgttg tcatacaggg tgttatagcg caccgcaata
     4801 aggtccatcc agttgccggc ttccggaatt tccagtaaac cgcattcatt catatcctgg
     4861 tactgcagcc aggtgagcgc gctggtgatt ctcggccagc tgcgcgccag cgcgtcttta
     4921 tcacctgtgg cgcggtaaag cgcgtactgc cccaggatgt accacaggtt ggcatcgcag
     4981 gaaccggcat tctccgtgct gatatccccg ctttcagggt tgaggttaag cgggatcatc
     5041 ccccagcagg attgcgcgcc gctgagtgca tccagcgaag ccctgccagc gttgatcagc
     5101 gctttatctt cactggccag cgcgcctaag agcgtgaccg aactatcgcg actccatacc
     5161 tggggatacc caactggatc cgctgaagca cggaaaccgt acggagtgac gcactgatgc
     5221 agtatttcaa gcgcgtgttt ggccgcttca ttgaccaggt ttttttcttt ctgattcatt
     5281 tattcctcca ccgccattct gctcatcctt taacggcacc gccaagcatg ccgcgaataa
     5341 agtaacgctg gaacacaata taaacgagca ggattggccc ggcgacaatc agcgcgccag
     5401 ccgccatcag cgggatattt gccgaatagc gcccctggaa aaaggccaac ccaaccggca
     5461 gggtacgcat ggtctctttt tgaatcatca ccagcgccag caggaactcg ttccaggtcc
     5521 acataaagat cagcaccacc aaagtgagca ccgccgggcg cgccagcggc caaagaatac
     5581 gccacagggt ctgccagcga ttgcagccgt caatcgcggc ggcatccacc agttcagaag
     5641 gcgccgtgct gaaaaaactg ctcatccaca acgtgccgaa tgaaacactc aacccaatct
     5701 gcggcaatat caaagcccag taggtatctg tcaacttcag gttatccatc atatgataaa
     5761 gcggaataac caccgcctca aacggaacca tataccccac cagcaacaat gggtaaagca
     5821 cctttttacc cggcaccggc agcatgccaa aagcgtaacc cgtgagcgtg gaaaggaaga
     5881 cgctgatgac caccaccaca accgtaacaa tcacgctgtt gcggaagaag aaattaaaac
     5941 gccctgtggt ccaggcttct ttgaaggaag cgaatctaag ctgcgagggc agtgtgagtg
     6001 gtccctttat cacctcgatg tcggttttaa aggcgctgaa gaccaggcca ataatgggca
     6061 acaggatgag gagcaaaaag gcactgagaa tgaggtaatt gggcagcagg cgctgccagg
     6121 cctggcgctt cataccccct ccccatctct ggattgaaaa cgcagtatga tcagcgaaat
     6181 gccaaaaatg agcagcgtca tcaccgtggc aaccgttgaa gcgtaaccga gctgattctg
     6241 ttggaacgct gagcggtaaa ccaaaaagcc ggtcaccagg gtcgaatccc ccggtccacc
     6301 gcgcgtggta acgtaaacca ggtcaaaaac gcgtaacgcg gcaataatgg tggtgataag
     6361 ggcaacaccc agctcagcgc gcaggctggg cagtgtaata tgcagcaact gacggaattc
     6421 gttggcgcca tccagctcgg cagcctcgta ctggtcattg ggtatgcgct gcatgcccgc
     6481 caggaaaagc accatacaaa agccgtactg cacccaggta cccaccgcac ctactgaagg
     6541 tagcgccagt tgaaaatcgc ccagccaggc gcgggtgaac gcccccaaac caatggcgcg
     6601 cagcccgaca ttcaacaagc cgaagttcgg gttaaagatc caccgccagg taacccccac
     6661 caccaccata ctgagaatct gcggcaggaa taacccggtg cggaaaaatg tcattccagg
     6721 taatggctta cgccctaata atgaggccag caacaagcca agaagaatgg ggataatact
     6781 gtaaaaaatg atgaaaacca agttgttaag caacgcttta agaaactgcg catctgtgac
     6841 cagtgaaatg taattttgaa agtccacgaa aacccgctcg cggctaaagc catcccattt
     6901 ataaaagctg gtgcggaagg tttcgatgat cggataaaaa acaaaaacca ggtagataag
     6961 caaacccggg aagagataaa gccaaccgtg aaaccgggat tcgcccggcc atctgcgctg
     7021 ggatttcaca ccactgctcc tgttgaaaaa ttcgcccgct tccccaacct ggcagaagcg
     7081 ggcgaacgtc agcgaggatt agttgccagc caagaaggcg gcgtaatcca cctcgacggc
     7141 ttttgtgaat tccgccggcg tgatgacttt gccgaacagt ttctgcagtt ccgccacaat
     7201 ggtgtcatag aacgtaggtg tcgcccagtc aatataatga ccaacagcgt tctctgagtt
     7261 gatcgtgtcc caggcgatca gtgtatcgtt gaacagcggg ttgccctccg ccacgtaacc
     7321 ttccggcaga ggcatggcag gcagcatgcc agcgttggcc cacaattcgg ccgcgcgcgg
     7381 gctggtcatc caatccagat attccgcggc caggtcggcc ttggtagtgg tcttgcggat
     7441 agagaaggga atccccacgc cgccaatcgc cagccgggta tgaccgggat tcgccggcag
     7501 caggaagaat ccaaattcct gatcggtgtc gccaaccagc tcacccgcca gccacgagcc
     7561 ggtgatggtc aaaacgccgc caactccggc tttaaagagc ttattgctgt cgtcatagcc
     7621 aatgccagca aaatccgggc tgaaatagcc cttgtcaacc caatcctgca tcacacgtgc
     7681 tgcttcctgg ttttctggag tgtcaaatgt gacattgttc actccataga cgaaatcgtt
     7741 gatgtaatcc agagaaacca gcaggtgctg cacggcgctg aactcgtgaa tggcgttcca
     7801 gccatccagt gagccaaacg aaataggtgt cattccagcg tctttgatac ccgcgaggag
     7861 ggtttggaac tcatcaaacg tagtaggaag cgtccagcca ttcgcatcca tgatggtctt
     7921 attataaaaa acacccacta cctcagcggt ggggctcaca ccatacaggt ttccctgacc
     7981 aaacgtcaca ccgtcggggg taaagctgtt gcggcttgcc accgatgaag aaaaacgctc
     8041 atcccattta taggcagcgg cgtaatcatt gagcggcagc agcaggtcag cctgcaccat
     8101 ggcgcccatg tcgctgcgtc cctggttcac ctgagccacg tcaggtccat cttcctgcgc
     8161 gagcgcgagc ttaagggtca ctttcagatc atccagtgtc ttgaccactc gttcaatctt
     8221 aacccctgga tgcgcggctt caaattccgc gttcaacgtt tccatcactt cactttcaac
     8281 gtcgcgatag aactgatccc aaaccacaag cgtcacttct tcgggcgctg cgggtgcttc
     8341 tgttaccacg ggtgcttctg tggcagcagg gacctcaggg acggatggcg cttcggttgg
     8401 ggccggcttg gcgcacgctg acagcaacag cgcgaaggcc attacaactg acaaaataaa
     8461 agtaatcctc ttcattttct ctcctttttt ttggaaatca tttttctggc ttgcaaccag
     8521 aacagatgag tttttcaact ctcttaaaaa tattttagta gagagtaaaa agaaagtcaa
     8581 acagctattg taaaggggtt gtggggaggt cagcacatcg tatacagttt ttaagcaacc
     8641 acgagggcgg gttatggtag tattatcgac agcaaaggaa gaaggaatga ccctcacctg
     8701 ggatgtcttt ggaattggga ctgccgcagt ggatgacctg ctgctggtgg agcgttaccc
     8761 tgaagccgac agcaaggtaa acatccttga atccatgcgc cagggtggtg ggcaaacagc
     8821 caccgccatg gttgcggctg ccaggcaggg cgctcgggcg gccttctgtt gccggctggg
     8881 gcttgatgac ctttcgcgtt ttaccttgag cgaactgcaa aaagaaggcg tggagacttc
     8941 agcctgccac caggacaggc aaggcagccc ttatcattcc atcatcattg ttgacacgca
     9001 aaaacacacg cgcaccattt tccacagcgg agggaatgtc agcccaccgc cagagctcat
     9061 taacgccgag ttaatctccc gcaccagggt gttgttcgtg gatgacaact caggcccggc
     9121 ggggatcaaa gccgcgcgta ttgccgcttc acttggcata ccggtgattg ctgatgtgga
     9181 acccgacccg ccgccagagg tggtggaact gctgccattg gtcgatcacc tgatcattgg
     9241 cagcaaactg gctcaaacat tgagcggtga aagcagcgaa ccagccatgg cgttcgcgtt
     9301 atgcggtacg caacgcgcct gctgcgtggt caccgcaggc gaaaaaggct gctggtacgc
     9361 gcaacggggt gcttccgcag tgcatgtgcc ggggcataag gtagacgttg tagacaccac
     9421 tggctgtgga gatgtcttcc atggggttta cgcggctgcc cttgcccgcg gagacagcct
     9481 gccagacgcg gtcgccctgg caaatgccag cgcggcgctc aaagccacgc gccccggcgg
     9541 acggttagga atccctgacc tggaaacagc acgcgctttt ctccacggta gtcacaccca
     9601 aaccctggaa ggcttaactc atgcgtgaca ccaggcagac attgattgac tgaaggagga
     9661 aaatcacatg cgagaaatct ggcaggttgt acagcagtgg caggcagaaa aacaacaggt
     9721 tgccctggct acagtggttc aagttaccgg ctcctcgttg cgccccgaag ggtcaaaact
     9781 ggtcatctcc agcggcgggg caattcacgg atcggtcaca ggcggctgcg tggagggcgc
     9841 ggtattcgaa gaagcgcgcg aagtattgaa agacggagct ccgcgcttat tgagctacgg
     9901 cgtcaccaac cagcaagcct gggacgtggg gctttcttgc ggcgggtcag ttcaaatctt
     9961 ggtcgagtcg ctggacacgc ccgattggca ggctgtcctg ccgcccctgg cacgcatatt
    10021 ggctgaaaac cagcttgccg cgctgcttac cgttattcac ggtgacagtg tcggtaaaaa
    10081 aatgttggtg ctaccagatg gcgaaactca cggcagcctg ggaaacaggg aacttgacca
    10141 ggaagccatc ggcaacctgc cggaaaactg ggcgacacgc ttacctttgc aaatcacatt
    10201 aaaaaatgga gaagtgttat tcgcggattt tattgttccg ccgccgcgcc tggtgatcat
    10261 cggcgcctca catattgcca ttccattggt tgcgctggcc aataccctcc aatttcatac
    10321 catcgtggta gacgcgcgtt ccgcctttgc cacgcgtgaa cgctttccgc acgcgcatga
    10381 gttggtcgtc gggtggccgg ctgacgtgct gcaacagttg aaactggatg ctgcaacctg
    10441 cgtggtcgcc ttgagccacg atgacaaact ggataacccg gcgctgctgt tcgccatcac
    10501 cagcccagcc aggtatatcg gcgcgctcgg ttcacgcaca accaacgcca aacgattaga
    10561 agacctgcgg gcggaaggcg ccaaagaaca agacctcaaa cgcatccacg cgccggtagg
    10621 gttgaaaatc ggcgcgcgtc aaccggaaga gatcgcatta tccattatgg ctgaaatcgt
    10681 ggcgaccgct catggccatg acggatagta caggaaggca cgaccacaag aaagtagccg
    10741 ccatcctttt agcggctggc ggctcaacgc gcatgaggca gcccaaattg ctgcttcact
    10801 ggcatggtca acccctggtc cgctggacgg cgcgactggc gctgtttaca ggttgtgagc
    10861 ctgtgcttgt ggtcaccggc gccaacgcgc aagaagtgca aacggccctc gcagggttgc
    10921 cgctgatctt tacgcgtaac cccgattggc aaaaagggca aagcacttca gtgaaggcag
    10981 gagtgaaagc gctgccagca gggatcgacg cggcgctggt ttttttgggt gaccagccgc
    11041 acatccctcc gcgcgtgcca cgggagctgc tgcgtgtttt tcaacaggat gaccccgcgg
    11101 aagccatact catccccact tttcaaggta agcgtgccaa cccagtgctg ctggaaaagc
    11161 ggctctttga agcgcttgcc aggctgagcg gggatgcagg ggcacgcgcg ctgttttcgc
    11221 aattcccggt caaacaggtt ccgtttgaag aacctgacct ggcttttgat gttgattcgc
    11281 ccgaagatta tcaaaagctg ctcgccaggc ccgcccccct gttcaaggac tgacccgttg
    11341 ctggataaaa aattccccgt caggtacaac ccccggggaa tatgcatgaa ggcaaaagcg
    11401 ggttagcgcc ggcggcggat gatgagaagc agcataagca ggataacggc gccaacgaaa
    11461 gcggatatga ccgcgccaag aatgccattt ccaaggttga ttcccagcag actaaaaagc
    11521 cagccaccca cgaatccccc gaggataccg acgatgatct taccgagcaa ccccatccga
    11581 atccctttta ccacccagtc agccagaaaa cccgcgatcg cgcctaccag aatccaaatc
    11641 agaatattca ttttttactc cttcctgtat ctttcataac aaaattatag tacgaaatca
    11701 tcgctcttcg cgaaaacagg tgaacagcgc gttgaaagcc ccacgcccgc gctcctggcg
    11761 ggatgaagtt tcgcctggct caactccaga taccttcttg aacgaattca tgtttgatca
    11821 agtgattgag ctctgaacgg atgcgcctgg ggcgaccatc catctccaag cggatattat
    11881 ccacatagta atgcagctca agttcagcag aatctgcgct tacattcttg atttctacca
    11941 tagggtcttt ccattcaccg ggcacgatct tatattcttt atccagccag tcaatcagaa
    12001 ccgctgaaag atcatccagg cgtaactcta cgcgttcatc agggtttgta atacagttct
    12061 tgaggttgtt ccaatggcgc agcaattctt cgttacgtga atcccaaagc cggcgctgct
    12121 gccagtagtc atgatcattc agccagcgat caggcgtggt cgaccagtca cgacattgct
    12181 ctgccagcgt cctcactgtg ttatccacag ggttcacatg ctcgttcaaa atctcggcca
    12241 cttctgcgcg gctgagcccg ctggtctctc ttgagcggat gctcctgatc atattgcgaa
    12301 tctcttcctt gagaaggaca agctggtcat tcaccctgcc ttcgcgagcc aggcgctcga
    12361 tggcagattc attatccgca gccattttct ccaattcaaa acgggtgggg tcctccgcag
    12421 aaagtccccc tgcccacgcg cgaatttttt ccgcctgtga aaggatggag gcggtttttt
    12481 ttgccaggtt ggaagccacc acgccagggt ggccggctgc gatgcggtac aacgtgtctt
    12541 taacaagcgt aaggtcgttc gaagcctcca cgctgaccga aacactgcag cgttgttccg
    12601 cagtcggctt ggtgaggttg acaaggtaat cagccgccag gaccttattc ggcatgtaaa
    12661 tcgtcgcctg ctcgtctaat gaaagcaggg tggtagagcg taaaccaaga cgctgtacct
    12721 cgcaaacttt gccgctcacc agtcggatga ggtcaccacg cttgaatggc gcttcgatta
    12781 acaagctcag gccgctgaaa atattggaaa gcgtttcttg cagcgccaga cccagcacca
    12841 gccccaacac ccctgccccc aataaaactg agctgacgtc tacaccccac atgggtaata
    12901 caatcaatgc gccgatggtg aaaaggagca acgggccaaa gaggctgagc accggcagca
    12961 agatgtcatc cacctggctc tcggtgcgcc gtgcccagct ttcgccataa tacaccagca
    13021 catcacggat aagtcgacca gcgatatgac cgaccagcgc cacgataacc gtcatcgaca
    13081 ggcgaatcaa caggcgctgc aaatcttccg ctgcctgcag atagcgaatc gatacgcgta
    13141 taccgatgac aagcaccagc agcgctagcg ggcggcggat gattcccaga acgatatctt
    13201 ccacctctcc aggcagtgcg agggtgaagc gcctgatcaa aggcatggcc acccagtaaa
    13261 taaaagccgc caataccatc caggcggcaa tattcaccag gaacacaggc aggggttttg
    13321 ccagaaaact cagttgcggg ggtaacggga tggggaaacc tgcaagtgtc atcttacact
    13381 cgttatttag atatatacta tcttatttat aatatatcac aattccactg aatttcttct
    13441 tcagtttacg cgggcattcg ctaaagaaaa cgtgttgata tttcgtctta atgacaaata
    13501 tccccaatat tagggtaata atccagtgtt attatttccg ctctcaatgt acattatcct
    13561 tgcgcgtcga aggttatctt tccccctgtg ccaacagggc ggaagttatt aaaggagaaa
    13621 aaatggattt ttcgtttatc gtgattatcc ttcaactgat cttccttgaa ggcattctat
    13681 ccattgataa tgctgccgtt ctcggcgcaa tggtcacacc gctgtctgac catgaaccca
    13741 tcccctggcc aaaagccctc gctgggtttg ggcacgcgat gaacaaaatc cttggacctc
    13801 agcgcaccgc ggctttgaaa gtcggcttga tcggcgcgta cgcaggcaga ggaatcatgc
    13861 tggtggcagc cagttatgtg attcaaaacc cctggttaaa actggttgga gcggcttacc
    13921 tgatcaaact ggcgttggat gacctgagcg cgcacggcgc cagtggcagc gatgaagatg
    13981 gtgaccgccc ggtaaaggaa cgcaccttct gggccactgt gctggcggtg gaattgatgg
    14041 acctggcctt cagcctggat aacgttgtgg cagccgtgtc actttcaaaa aaactgtggg
    14101 tggtcatgct gggtgtggca attggtattg taatgatgcg ttttgcagcc agcctcttca
    14161 gcaaagtggt tgaaaaagcc cccgtcttaa aaaccgcggc ttacatcctg gtactcaata
    14221 tcggtgtgga gctgctcatt gaagacctcg gccatattga aattggtgat tggacgcgct
    14281 tcggtatctc catcgccacc attctgctcg ccctggctta tgagcgtttc aagtttatga
    14341 aattcttccg cccattattg atctggttga gccaggggtt cgctttgatc aacggtatca
    14401 tcacctggat cttcgctccc atcaaaggca ttttcacgct cgtgagcaaa ctttttcttc
    14461 gccaacgggc tactcccagc gtggctgaat aatcaagaac acacccccgt gagtacaaca
    14521 cacgggggtg gttttttatc aacaggcagt cgcggttaga gctctttctc catccatacc
    14581 acctgggtcg tgccaaaata ccgggtaggc agaccgtgca ttttgtaccc tgctttctca
    14641 aagtaggcgg tcgtgttcaa gcgtgcctgg cagccgatct tcttaaagcc ctgttcacgc
    14701 gcctgggctt caacgtgcgc caataactgc cgcccaattc ccgtgcgctg caaatcggtt
    14761 tttacagcca gatgcgagac ccaggcaacc ccgggttctt tttcagaaaa cttgacacat
    14821 cccactactt ctttagtttc atcgtctacc gccataaagt gttgtgagat gggttcataa
    14881 tcatcctttt cggtgccgcg tggttgaccc cacggttcac gtaatacctt atagcgcaga
    14941 tcatagtagg ctttaaaatc ctctttggtt ttgggtgtcg ttatctgtat caattgaata
    15001 cctcgctttt agaatcgacc tggttgaatt atacagcaga tttatttcac gctatttctt
    15061 tgccggctgg gttttcccgt tgatggtgtg agccacctcc accttcacgg tttccatcaa
    15121 ggctttttca gtgaagcaca tctggcgggc aaggtcaatc aatttttgga tttctttttc
    15181 cggcgcgtca gaatcaacca ccacctctat ctcaaaatgg tcgcaatacc cctcagcgtc
    15241 accttttaac accgaaccct gctcatgaaa atacgcgctg accttcactc tttcgttacg
    15301 gatttgcagt ttctttttgg tcgcaacctg cgacacatga gaaagcagtc aaagcgccat
    15361 tccgctgacg aaatacgtca gcggagaagg agcagtacct tcccctcccg gcatgtaggt
    15421 gccctcatcg ctgatgaaag cgtaagcccg tccggtttcg ggctgaccgg caatcacctg
    15481 tttacgccac ttgtccagcg aactggcctc agcgttcacc ctgattgcgc gaaaacccag
    15541 tggttttttc tctgccatac ttcctcctgt gattcatgga tgatataagc gctccgcgag
    15601 cgccttttga tagtcgttca aatccaacga taagcgtgcc gcgccgctgc gcattgccgc
    15661 ctgcgccacc gccggcgcct cccaaaggca tacacgccta tcgagtggtt tgggcagcac
    15721 ataatccttg ccaaattcca ggcgcttcag cccgtaggcc ttcagaactt ccgccggtac
    15781 ctcctcgtgc gccagcgctg ccagcgcctg gctggcagcc actttcatct cgtcattgat
    15841 ggtagtagcg cgcacatcca acgcgccgcg gaagaggaag gggaaacaca gcaagttatt
    15901 cacctggttg ggataatctg acctgccagt gccaagcagc gcgtcaggcc gcgcctgttg
    15961 agcaagttca tagctgatct cagggtcagg gttggccatg gtgaagataa caggttgagc
    16021 gcgcatgcgc cgcagcattt caggggtgac gatattggcc gctgaaagac cgataaagac
    16081 atctgcgcct tctatcacct ccgctaacga ggcgggcttg tctccttgcg cgaaaacctc
    16141 tttttcagcg aacatctcct ccaccctccc ccggtagacc aacccggcga tatcacacat
    16201 ccagatatgt tcacggggaa tacccatctt catcaaatgg ctggcggagg cgagcgcggc
    16261 tgctcctgcg cctgaaaaaa cgactttcac ttctgtgagt gacttgctag tgatttcaag
    16321 cgcgttgatg agcgccgcgc ccaggatgat ggccgtgcca tgctggtcat cgtgaaagac
    16381 cgggattttc agcatcgtct ttaattgttc ttcaattgaa aaacattccg gtgaacggat
    16441 gtcttccagg ttgataccgc caaaagaatt gccaatggca gcgcagacgc tgatcaccgt
    16501 ttcgggttca acagagttga tttcgatatc aatgctgtca atatccgcga attttttaaa
    16561 cagggccgct ttgccttcca tcaccggttt actggccaac gcgccgcgat tgcccaagcc
    16621 gaggacggcg gtgccgttgc tgatgaccgc caccaggttt cctttggtgg tgtactcata
    16681 cgccagttcc gggtcgcggg cgatttccag cacagggtac gccacaccag gggtataggc
    16741 cagtcctaga tgatgttgat tctcaattgg cttgcttggg tgaatgacca gtttccctgg
    16801 tactccgcca agccggtggt aagacagcgc gctttcttta tcaatagggt tcattcaacg
    16861 ccttcagggc ttacaaaaac tccaccaaac ctgaccttca gcggctcttc aaatgggcat
    16921 gcggcaaacc cagtctatca cagtttgttc gttgcccgcc tccagttagg aggtagtgta
    16981 aaagagagtg ttcgtaacgc acccattaca ggcatcaatg ccgatgcgct ttatgttttc
    17041 catttctatc ctgagatttt ttgaattctg taccgatgtg aaggcccagg cgagggaaaa
    17101 tgacccgatg caccagaagg atgagaagcg taacgccaaa cgcgatcaca aaaacgatta
    17161 atgattttcc gatatcgctc atcaccgccg ggtttaccag catcacaatg gccagtgtca
    17221 gcatcaatac cccgccaatc aatttcaaga tgcgtccatg tttctcttcc atcttggagg
    17281 ttttaagggt gaaaaccgcc acaaaaaaga tgcccagttc atccagctgg tagatcacca
    17341 aatacaggag caacaacagg atgaaagtga tcacagagac ctgatgcgca gccaccaggt
    17401 tcgtccacaa gacgggaaaa cctgccgtgc aggaaaactc aaccagcgaa acgcccgccg
    17461 ccagcacgat cgtcccaccg atcaaccccc accatgagtc cccggcatcc atcactttcc
    17521 gcatacgctg gaagaggcca ggcttcttct catctgaaat ggtaaaagat actccttctt
    17581 tatagaagaa gtagtctttt atattgacca ggccgaagaa gagcgccacc agccccacca
    17641 ctacctgtat ccaacccaca aaactgacca ccttcagaac ggtgaacaac ccggcaatga
    17701 acaacgcgta aatggcagcg gtgacggtaa caaaaaccaa accaatgacc agcacttttt
    17761 tacgtgaacc ggtgtgcagg gtgatcgcca gcagcatgct caacacccaa atcgaacagg
    17821 ggttaaaacc atcaacaaag gagatgagcg ccgtgctgag aaaaagtccg attttggtga
    17881 agtccacctc accaaaaaac ggtagtttag aggttgcgcc gttgttttcc gtgttcgtat
    17941 cgggtgattc agtggctgcc ggagtcggcg cggcagatgt gggcacagcg gttggcgcag
    18001 cggcattttc gccaaacggg acatagcctt tccccgcgat tagcgcgctg cccgggtttt
    18061 cgcacgcttt ttcaaggcat tgctcaacta caccggtgat ctcctgcgaa aattcatcag
    18121 aataaccttc ccaaatattt ttgccaatca aaatcgtggg aacataacgc ggcgtaaaac
    18181 cgtaagcctc gccaaattga gagaagagct cctggttggc ctgagaatta tagatttcga
    18241 actcatacag atgtgctgca gggtattttt ttgccagatc ctcaagcacc ggtttggctt
    18301 tcgcgcagtg cggacaaccc tcgccccaga aaaagtaaat atcgacttcg ctttcagcct
    18361 gcgcctggat ttg
//
LOCUS       DAOSPM010000034        18316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_3049_length_18316_cov_11.940365, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000034 DAOSPM010000000
VERSION     DAOSPM010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18316
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3049_length_18316_cov_11.940365"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..3019
                     /locus_tag="PLW25_04565"
     CDS             149..3019
                     /locus_tag="PLW25_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS53795.1"
                     /translation="MRKLYAILGVLIAATMVLSACAKPTAAPTAAPEVPAATEAPVVE
                     TAIPHNGKGAWLDKVIFTAVADADSAVAQLQAGAIDIYPVSVEDPEVFAKVKADENLG
                     YATVYGSSNQLMVNVVKCDDGSLNPFTDMEFREAMNWAFDRDYVVQEFFGGLAIPKFT
                     SFTGAFPDYARYADVMAAITSTYAYDMEKAQAAVDARMTALGATKNASGVWEFNGAPV
                     TIKVVIRTEDQRLGIGQYFASQLEALGFKVERLEKTRTEASPIVWSATPELCEWHVYT
                     GGWISTAISRDDGYQIPQFNTGLVQTTLPIFSKYDPSPEFDVINQKLLYNDFTSMEER
                     DQLIRDGLNLAMKESWWGVWVNDNTAISPYRKPLEGAYDLAGGFASAPLWPYTMRWAD
                     KVGGVVRVAQSGILVQPFNPINGSNWTDDSMVYRGIMDWGLVPNPFTGLNMPKLVEKA
                     ELVAKTGTPIIENGEDWFKLSFQDAVEVPEDAWADWDPVNEKWLTVAEMKVKVEEAKA
                     KAAEIEAVKAEVEAKRAELVAAFDTTNMTVETMAAVTKEFTDFAAEKYGLPWDASAFV
                     DAELPGEVEWIDGNLAADAVADRQGELDWWLGYVAESTDPSVSVVDLAGRDYSTAVTK
                     SVVIYRPELWDTTWHDGSPFTIGDVVYGMIMNFAPGKEGSPIFDSTMKGPVDAYLSHF
                     RGVKITSVDPLTIETYDNAFALDAENSVSAWYPSFSYGPVAWHDLTPAVLAESDEKIA
                     FSKAKATQLGVDQTSLIAGETLAIQKKYLDEAAAAGTIPFAPTLSEYITADEAAARYA
                     NLLGWFQDKGHLMIGTGVFYVDEVYPTEGTVTLSRYADYIFPSDEFSGFGAPMIADAE
                     VDGPLAITKGEAADFTVAVTFDDEAYPSAKLNFVSYMLFDATGKLVEKGNATMTEEGL
                     YAVSLSAETTGKLEVGGAKLLTAVSSKVVSIPTFSEYEFVVQ"
     gene            3095..4243
                     /locus_tag="PLW25_04570"
     CDS             3095..4243
                     /locus_tag="PLW25_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS53796.1"
                     /translation="MVDASPTIDSFNSLEQEVIKEKRSSTALRILKYTASRMVMMVIT
                     VTIGVYLTVLIANMGGYVDTIRKAQIREQVGLIVANDPVLRKLSPEVRNIRIAEMVRD
                     QESIYGLDKPFLVRSFLYLKQALTLDLGRAMSMTSNSGSSSVRNIIIERLPPTLLLFG
                     TSDLVLFFLALMIALSLSRHYGSVMDKIVIALTPLSSAPGWFYGIFLILIFAAVLRVL
                     PFGGMVDAPPPKETMAYILSLLRHMLLPVAAITISSIFLSVFSWRTFFLIYSSEDYVE
                     MAKAKGLTERDVTQKYILRPTLPTIITNFALLVIGMWVGAPIFETVFQWPGLGRALYQ
                     AIGLFDTPVIVAEAVIFSYLLGITVFLLEIVYALVDPRVQVGNEGPRA"
     gene            4240..>5144
                     /locus_tag="PLW25_04575"
     CDS             4240..>5144
                     /locus_tag="PLW25_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53797.1"
                     /translation="MNSIKRTFNELFKYPSAVAGLIIILLLIALSIYTLIALPYDRAI
                     SLWRGESKDWYASPKYAMPAWINSFRKNKLPTTIILKTDDPSVVKTEEKATVIYEMPF
                     VFTADAFPSEINVFFKSTYTSSPPFVGLSIITPDEREIKLGNFKVERSLAYYLSQDQK
                     LVKKYKDIPIQEALLMQPDSKPRKPLKGTYKLIIEGVTFDEGSTVSAEAVFYGKVSGI
                     AGTDHKRRDLSIALLWGTPIALMFGLVAAVGTSLTQLIISAISTWFGGWIDVLIQRIT
                     NVNMVLPFLPILIMIGTFYSRSIFV"
     assembly_gap    5145..5244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5245..5433
                     /locus_tag="PLW25_04580"
     CDS             <5245..5433
                     /locus_tag="PLW25_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53798.1"
                     /translation="LGDPTIPTWGKVIDESYNSGALFNGYYYWVLEPSFLLVVTGLAF
                     AVVGYALDRVFNPRLRGQ"
     gene            5433..6392
                     /locus_tag="PLW25_04585"
     CDS             5433..6392
                     /locus_tag="PLW25_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53799.1"
                     /translation="MANSPTLLKIEDLVLYFKTMRGPVQAVDHIDLDLKNNEAIVILG
                     ESGCGKSSLAKAILRLLPRNIDKYSGKVLLNGTDTMQYSDEEFRRKVRWRSISMVPQA
                     AMNSLNPVLKVGDQVTEPFIIHTGASKTEAIAQAKKMFAHVGVPNSFLDRYAFELSGG
                     MRQRSAIAMALVTMPDLIILDEPTSALDVLTQANIFNVLKTLKKDLEISFILITHDIA
                     TSSELADRVAVMYAGQIVEVCDATRFFDKPFHPYSIALMDSVPRLRQVKEPDFITGQP
                     PSLINPPTGCRFKDRCHKRFEKCEEEPPAVTIENHIVKCWLYQ"
     gene            6407..7444
                     /locus_tag="PLW25_04590"
     CDS             6407..7444
                     /locus_tag="PLW25_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53800.1"
                     /translation="MDHIIDKNEKAEPFLQQDIRERGVLLSIRDLKIWFELRKFGFGH
                     AGYVHAVDGVSFDLHNGESIAVVGESGCGKSTLMKGILGLHRPTEGEIFFRGESIGEM
                     KSKEIKQYRSHIGYIQQDPYGAMAPFMSVQRILTEPLSINGIKDHQQIQDMVHKALEE
                     VKVFPVEDYLNKFPHMLSGGQQQRIVIARSMILNPNFLVADEPVSMLDASVRVEILRL
                     LRSLQESRNLAVIYITHDLSTVSYFSRRIFVMYAGQIVEKALVQDLLNNAGHPYTQAL
                     LTGTSDPDGANAHKLKEVPPGEPPNLINPPKGCRFHPRCPKIIRGLCEVEAPPEFEPE
                     PEHSVACWLLR"
     gene            7441..7677
                     /locus_tag="PLW25_04595"
     CDS             7441..7677
                     /locus_tag="PLW25_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53801.1"
                     /translation="MIIDRLNPRKLIWSAIILMVMGVAIPFLMVIHVLDPLLRWNSTI
                     YLILNFVAYFFQILGFILGIIGIAIYTRRNKHRE"
     gene            7795..8889
                     /locus_tag="PLW25_04600"
     CDS             7795..8889
                     /locus_tag="PLW25_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOS53802.1"
                     /translation="MNAIGSTAITILEFILAFGALVLLHELGHFFAARLSKIEVEEFG
                     LGYPPKMFRLFRAGGTDFTINWIPFGGFCRMKGENGEVSEPGSFLAARPSRRLLTLLG
                     GPLTNLLVGMLIISFLFTRIGSPDPSSVMVAAVTDETPAAAAGILPGDLIQSVNNVQI
                     TSMDALSKVISSHLGQEVEIVLLREGVALTIKVTPRAEWPEKQGPLGITIMNPSQKVS
                     FIQALPRAFIATLDQGYQLLMVPVRLISGQIAPSEARMVSVKGIYDIYAQVQTLDKQE
                     AVENPAMAGLNTLYFFAIISIALGFTNLLPIPALDGGRILFLLPELFFKKKIPPALEG
                     KVHMIFYALLLTLMVLLVINDIVNPVIIPK"
     gene            8897..>9714
                     /locus_tag="PLW25_04605"
     CDS             8897..>9714
                     /locus_tag="PLW25_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOS53803.1"
                     /translation="MNKESGEPIPFSSVLSALQNASVPFPARYLRSFSDLPPANLKVL
                     LNCWPSIPARRKVSLLEDLEAVLEEDTLVNFDNLARALLADPDPHVRTCALRLLWECE
                     EKSILPILVDISIKDEDETTRETAISLLGKFVLLGELDSLKDSAKEGLNQHLVNIVRG
                     KDKTRVKQRALESLGYSSHPAVPDLIRNAYQTNDLNWVTSAICAMGRSADDAWGPQVV
                     AMLTSPEPEIQFEAIRAAGELELRTAREPLLALLEEETEDEEMRLALIWALSQIG"
     assembly_gap    9715..9814
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9815..11817)
                     /gene="uvrB"
                     /locus_tag="PLW25_04610"
     CDS             complement(<9815..11817)
                     /gene="uvrB"
                     /locus_tag="PLW25_04610"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOS53804.1"
                     /translation="MQFHLQAPYQATGDQPEAVKQLIKGIRQGMRNQVLLGATGTGKT
                     FTIAKVIQELQMPALVMAHNKTLAAQLYAEFREFFPQNAVEYFVSYYDYYQPEAYVPR
                     HDLYIEKETDINDEIERLRLAATTALMSRKDVIIVASVSCIYGLGNPEEYSNVVVHLA
                     VGEIFRRNALLRRLIESQYQRNDLELRPGAFRVRGDTMEIFPAYEEKQAVRITFFGDE
                     VEKIVEFNPMTGEIIAEPSAIDIYPAKHYIASEARLKVALDGIEKELNDQIHYFKANG
                     RVLEAQRIEQRCRYDLEMLREVGYCSGIENYSRHLDQRPAGSPPWTLMDYLPSEYLLV
                     LDESHMTVPQIRGMYNGDQSRKSTLVEYGFRLPSALDNRPLTFKEFERVMGYTIYTSA
                     TPADFELSRSDQIVEQIIRPTGLVDPEVEVRPTKGQVDDLYGEIKKRVEQGERVLVTT
                     LTKRMAEDLSDYLLELGLKVNYLHSEVETLERVGILRDLRMGVFDVIVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSATALIQTIGRAARNVNGKVIMYADTITGAMKTAIDETN
                     RRRKKQMDYNKANGIEPFTVVKAVHDLTERLSASSVIAEAKGRYSAGEEKPRMPISDL
                     KRMILELEKQMKQAASELQFEQAAVIRDRLIDLKTYLAEESDLP"
     gene            complement(11875..>12659)
                     /locus_tag="PLW25_04615"
     CDS             complement(11875..>12659)
                     /locus_tag="PLW25_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS53805.1"
                     /translation="GDFSTRVDIKGNDEIATLAKTFNQLARNLELAEERKQSLTADAA
                     HELRTPIAVQKAQLEGMLDGVLPLTQKNVLIALQQTNFLSRLVEDLRLLAMADAGEVR
                     LEMRILNIVNLVQQVVAGFQAQAALEGTNLVTVFRPDGEELWIHTDVDRVSQILQNLI
                     SNALRYGQKGGRILISTRKESAKLVISVQDDGSGIPSTALPHLFERFYRHERARTREN
                     GGSGLGLAISKKLALLLGGDLSGANSPEGGAVFSLELPIRNT"
     assembly_gap    12660..12759
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12760..13055)
                     /locus_tag="PLW25_04620"
     CDS             complement(<12760..13055)
                     /locus_tag="PLW25_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53806.1"
                     /translation="MKFSLQQKMILAFSVVVIVSIGGVVIFANLDSQRQVNRYLTRGG
                     QYGVMELVEALEDYYEYKGTWSMVGEVADAYSQRHGRKNAHGKASELTLTDQNH"
     gene            complement(13059..13790)
                     /locus_tag="PLW25_04625"
     CDS             complement(13059..13790)
                     /locus_tag="PLW25_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS53807.1"
                     /translation="MAGKLAEGSGCYNRFMTTILIIEDEVTLARILQSYLEREGYRVL
                     SAQRGDTGLDLCLSENVDLVLLDLNLPGMDGVDVATEVLREKDIPIIMVTARVEEIDR
                     LKGLELGADDYITKPFSPREVVARVKTVLRRVNRSRVEEDRFLIPPFEIDIDAHLVKK
                     AGQEIDLTPSEFAILVTLARHPGRVFSRLQLLEATQGQSFEGYERTVDAHIKNLRAKI
                     EEDARKPAFIKTVFGVGYKFYREDE"
     gene            complement(13742..17122)
                     /gene="mfd"
                     /locus_tag="PLW25_04630"
     CDS             complement(13742..17122)
                     /gene="mfd"
                     /locus_tag="PLW25_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOS53808.1"
                     /translation="MLSTLRQQAAYLQCLDSIKVGTQPASLGLLRSARLPVVAALCID
                     LQVPVLLLTDRADRASMLFDELRFWLPDFPRLYFPEPTPMFYEKAAWGSLTRRDRLQT
                     MAVLVNYFKLGSQKPDKPCVVIAPLRAVMTRTLPRRDYIIASKVLKINGHANPDTLLR
                     EWARLGYENTEVVVDHGQFSKRGGILDIWTPAEPYPVRLEFFGEEIDTLRFFDPATQR
                     TVSNIDQIQITPAREFIPDGDLKFSGEDGFVDEFQIPMIHPLSSTILDYLPPDALILV
                     DSLSNIQSFGEEIEEQALKNRAEVVREGIIPADFPVPYISWSEVLEMISNRRWMELLR
                     PNNEDMGSLGSKFSPGMRFAGQISHFVNYLEACCREGDEMVIVTRQLSRLKEIWEEQS
                     DACAHSGNLKFIESALGEGWDLELDSGKTLHCITDGEIFGWERPQPRARLTARYLSPE
                     ADYADLKKGDWVVHVDYGIGRFAGLVSRILDGMEKEFLLVEYENGDQIYVPIHQADRI
                     SRYIGTGGEPPQPSSLNGHEWNQTKQGVREAVAQVASELLELYAKRQSAVGHAYKPDT
                     AWQQELEASFPYIETEDQMKAILSVKQDMETPKPMDRLLCGDVGYGKTEVALRAAFKA
                     VMDGKQVAMLVPTTVLAQQHFETFRQRLSPYPVSVEMLSRFRTPKEQEEIVLRIMLGQ
                     VDIVIGTHRLLTSDVVFKDLGLVVIDEEQRFGVTHKEHFKKLRTEVDVLTLTATPIPR
                     TLYMALTGVRDISTINTPPAERLPIVTHIGPYSPRIVRQAVLREIGRGGQVFFVHNRV
                     QTIEAMKAHLESLVPEARVSIAHGQMREEELSRVMTRFTKGEVDVLLCTSIIESGLDI
                     PNANTLIVDRGDAFGLAQLYQLRGRVGRGGQRAFAYFFKHRKKAPTPEGQERLETIAE
                     NIQLGAGYSIAMRDLEMRGAGEMLGTRQSGYIASVGFHLYTRMLAQAVRELRLEQGQN
                     EGESENAIPSSGYIPVSVDLPISVGIPVDYVPDQALRLQLYRRLAHLEEVSELDALAA
                     EFRDRFGQPVQQVKDLFFQLRVKLLAAKAGLTSINVEADQLVLRFPQLPEGAVKRELA
                     PLGYQTRIGKNAYWMKFSPEENEWRENLLKVLGAIIAS"
     gene            complement(17124..17756)
                     /gene="pth"
                     /locus_tag="PLW25_04635"
     CDS             complement(17124..17756)
                     /gene="pth"
                     /locus_tag="PLW25_04635"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOS53809.1"
                     /translation="MSLLKIFYKKTSAIKESTEAVYLLAGLGNPGREYRETRHNIGFM
                     MIDSFAARHNIKLNKVQNKAIVGVGRVNGVRVILAKPQTYMNQSGQAIAGLVRYYKVS
                     LENLIVGHDDVDLPFQTIRIRPAGGSAGQKGMTSIIEQLGTQNFARLRMGIGRPPGQM
                     DAAAYVLQTFTKAEKEMLGSFLDRAVEALQCYLNEGLETAMNRFNPTEKD"
     gene            complement(17765..>18316)
                     /locus_tag="PLW25_04640"
     CDS             complement(17765..>18316)
                     /locus_tag="PLW25_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53810.1"
                     /translation="LLGMTITPGLYITAPTTGEVVEGIVEIRGSVPEEGFLSAEVLYA
                     YAQTGKETWFLIKRLDKVVQDDVLAAWDTTTITDGVYHIKLVVTKVGDRRNEVVVENI
                     QVSNYSRAPGQNEMGVNTQTEKRSAAVEIRAPVSTVIEKKVNPASIQRQEIQRTIIIA
                     ILAVCVALSSIAIYSAIQSRRRR"
BASE COUNT         4365 a         4749 c         4263 g         4639 t
ORIGIN      
        1 tttccttgac ttttttcatc aaatgtcata aagttaagct gtccgaagca gacaggtaaa
       61 gtaatcattt aaaaaggagg tgccacttaa ggatctcgct ttgttagtac caagcaagcc
      121 caaaattcaa attaggagga gaaactacat gcgcaagtta tatgcaattc taggagtttt
      181 gattgctgcg accatggtat tgagcgcctg cgccaagcca accgcagccc caaccgcagc
      241 ccctgaggtt ccggccgcca cagaagcccc cgtggttgaa acggccattc ctcacaatgg
      301 caaaggcgcc tggctggaca aagtgatctt taccgccgtt gccgatgccg actcagccgt
      361 agctcaactg caggctggcg cgattgatat ctacccggtg agcgttgaag atcctgaggt
      421 attcgctaaa gtaaaagcgg atgaaaacct gggctatgct accgtctatg gttccagcaa
      481 ccagttgatg gtgaacgtgg tgaaatgcga cgacggttca ctcaatccct tcactgacat
      541 ggaattccgt gaggcaatga actgggcctt tgaccgcgat tacgtggttc aggagttctt
      601 tggaggattg gcaattccga agttcacctc tttcactggc gcttttcctg attacgctcg
      661 ttacgcggat gtgatggccg ccattaccag cacctatgcc tacgatatgg aaaaagcaca
      721 ggcagcggtt gacgcccgca tgaccgccct cggcgccacc aagaacgcca gcggtgtttg
      781 ggaatttaac ggcgcacccg taacgattaa agtcgtcatt agaaccgaag accagcgcct
      841 tggcatcggt cagtactttg cctcccagct ggaagcctta ggatttaaag ttgagcggct
      901 agagaaaacc agaacggaag ccagtccgat cgtgtggagc gctacccccg aactttgcga
      961 atggcacgtt tacaccggcg gctggatcag caccgctatc agccgggatg acggctatca
     1021 gattccgcaa ttcaacacgg gtctggtaca aaccaccctg cctatcttta gcaaatacga
     1081 tccttcacca gaatttgatg tgatcaacca gaaactgctc tacaacgact tcaccagcat
     1141 ggaagaaaga gaccagctca ttcgagacgg tcttaacctg gcgatgaaag aatcctggtg
     1201 gggcgtctgg gtaaatgaca atactgccat ctcgccttat cggaaaccct tagagggcgc
     1261 gtatgacctc gctggtggtt tcgcctcagc cccgctttgg ccatacacta tgcgttgggc
     1321 agataaagtg ggcggcgtgg ttcgagttgc ccagtctggt attctggttc agccgtttaa
     1381 ccccatcaat ggttccaact ggacagatga ctcgatggtt tatcgcggca tcatggactg
     1441 gggtttggtc cccaacccgt tcaccgggct gaatatgcct aaactggttg aaaaagccga
     1501 actggtcgcc aagaccggca cacccattat cgaaaatggt gaagactggt tcaagctttc
     1561 cttccaggac gcggtggaag tacctgagga cgcctgggct gactgggatc ctgtcaatga
     1621 aaagtggctt actgtagctg agatgaaggt taaagttgaa gaagccaagg caaaagccgc
     1681 agaaatcgaa gcagtcaagg ctgaggtaga ggctaaacgc gcagaattag ttgccgcgtt
     1741 tgacaccacg aacatgacgg ttgaaaccat ggcagcagta acaaaagaat tcaccgattt
     1801 tgccgcggag aaatatggcc ttccctggga cgcatccgca ttcgttgatg ctgaacttcc
     1861 cggtgaagtt gaatggatcg atggcaacct ggctgcggat gctgtggctg accgccaggg
     1921 tgaacttgac tggtggttgg gttacgtagc agaatccacg gatccttctg tatcggttgt
     1981 tgacctggcc ggacgtgatt acagcacggc cgtaaccaaa tccgtggtta tctaccggcc
     2041 cgaattgtgg gataccacct ggcatgacgg cagccccttc accatcggtg atgttgtcta
     2101 cggcatgatc atgaacttcg cgcccggcaa agaaggcagc cccatcttcg actccacgat
     2161 gaaaggccca gtagatgcct acctgtctca cttcaggggc gtcaagatta catcagtcga
     2221 cccgttgacc atcgagacct acgacaatgc ctttgccctg gatgcggaga acagcgtttc
     2281 tgcctggtat ccttcgttca gctatggacc agttgcctgg catgatctca cccctgcggt
     2341 gctcgccgag agtgatgaga agatcgcctt cagcaaagcc aaagcaactc agcttggagt
     2401 tgaccagacc agcctcatcg ctggagaaac tctggcaatc caaaagaaat acctggatga
     2461 agccgctgct gctggtacaa tccccttcgc cccaactcta agtgaataca tcactgcaga
     2521 tgaagctgct gccaggtacg ccaacctgtt gggatggttc caggataaag gtcacctgat
     2581 gatcgggacc ggcgtcttct acgtcgacga ggtttacccg accgaaggta ccgtgacatt
     2641 aagccgctac gctgattaca tcttcccttc agatgaattc agcggatttg gcgcgccgat
     2701 gatcgcggat gctgaagtcg acggacccct ggcaattacc aagggcgaag ccgctgactt
     2761 tacagttgcg gtaacctttg atgacgaggc ttacccgagc gcgaaattga actttgtctc
     2821 atacatgctc tttgacgcga caggtaaact ggtcgaaaaa ggtaacgcca cgatgacaga
     2881 agaaggactt tacgcagtgt cgctctcagc tgagacgact gggaagctag aagtaggcgg
     2941 agccaaactt ctaacagcag tctcatccaa ggttgtgagc atcccaacct tctctgaata
     3001 tgaatttgtt gtccaataaa caaaactaat acattactcg gtaggggtat aaaaccccct
     3061 accgagtttt gcctatttaa ggagtaagtt cattatggta gatgcgagcc caaccataga
     3121 cagctttaat agtttagaac aagaagtaat taaagaaaaa agatccagca cagccttgcg
     3181 gattttaaaa tacaccgcca gtcgcatggt catgatggtg atcactgtta ccatcggtgt
     3241 ctacctgacc gtactgattg cgaacatggg tggatatgtt gacacgataa gaaaggcgca
     3301 aatccgtgaa caggtcggtc ttattgtagc caacgatcct gtattacgaa agctgtctcc
     3361 tgaggttcgg aatattcgaa tagccgaaat ggttcgcgac caggaaagta tttatggcct
     3421 tgataaacct ttccttgtga gaagtttcct ttacctgaag caggcgctca cgctggacct
     3481 tggcagggcc atgtcgatga ccagcaatag cggttcttca tctgtacgga atatcatcat
     3541 cgaacgactg ccacctactt tattgttgtt cggtacatcc gatctggttt tattcttcct
     3601 ggcattgatg attgctttga gtttatcgcg gcactacggg agtgttatgg ataaaattgt
     3661 gatcgctctc acgccgcttt cttctgctcc cggctggttt tacggcatct ttctcatttt
     3721 aatttttgca gctgttctgc gtgtcttgcc gtttggagga atggttgatg ctcctccgcc
     3781 taaagaaacc atggcatata ttttgagttt attacggcat atgctgctgc ctgtggcagc
     3841 catcaccatc agttcgattt ttcttagcgt attttcctgg cgcacatttt tcttgattta
     3901 ttccagcgaa gattatgtcg aaatggcaaa ggcaaaagga ttaaccgaaa gagacgttac
     3961 ccaaaaatac atccttcgcc ctactctccc aaccatcatc accaactttg cccttctggt
     4021 tattggtatg tgggtaggag caccaatttt cgaaacggtt ttccaatggc cgggtttggg
     4081 acgggcgctt taccaggcaa tcggattgtt tgataccccg gtcatcgttg ctgaagcagt
     4141 cattttttct tacctgctgg gtatcacggt ttttctatta gagatcgttt acgctctggt
     4201 agatccccgt gttcaggttg gaaatgaggg acccagagca tgaactcaat taaacgtact
     4261 tttaatgaac ttttcaaata tccctccgct gttgcggggt tgatcatcat cctcttgtta
     4321 atcgcgctat ccatttacac gctgattgcg ctaccatacg atcgagcgat cagcctttgg
     4381 cgcggtgaat caaaggactg gtacgccagt ccgaagtacg ccatgccggc ctggattaat
     4441 agttttcgca aaaataagct tccgaccacc atcattttaa aaacggatga tcccagtgtg
     4501 gtcaagaccg aagaaaaagc aacggtcatt tacgagatgc cctttgtatt cacagcagac
     4561 gccttccctt ctgagataaa cgtgtttttt aagtccacct atacaagcag cccacctttt
     4621 gttggtttat ctataatcac gcctgatgaa cgagaaatta aactggggaa tttcaaggta
     4681 gaacgctctc tggcctatta cctgagccag gatcagaagt tagtaaaaaa gtataaagat
     4741 atcccaatcc aggaagcctt gttaatgcag cctgacagca aaccgcgtaa gccactcaaa
     4801 ggcacctata aactcataat agaaggcgtt acctttgatg aaggttcgac agtcagcgct
     4861 gaagccgttt tctatggcaa agtttctgga attgccggaa ctgaccataa aaggcgagac
     4921 ttgagcattg ccctcttatg gggaacacca atcgcgttga tgtttggcct ggtggccgca
     4981 gttggcacct ctttgacaca actgattatc tcggctatca gtacctggtt cggaggttgg
     5041 atcgatgtgc tcatccaacg cattaccaat gtcaatatgg tgcttccatt cttgcccatc
     5101 ttaatcatga tcggcacgtt ctattcgcgc agtatttttg tcatnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnctggga gatccaacca ttcccacctg gggtaaagtc
     5281 atcgatgaat cttataacag tggcgcgctg ttcaatgggt attattattg ggttctcgaa
     5341 ccgtcgtttc tcttagttgt caccgggctg gcatttgctg ttgtcggcta tgctcttgat
     5401 cgcgttttta atccgcgttt gcgaggtcaa taatggcgaa ctcacctaca ctgctcaaaa
     5461 tcgaagacct tgttctctat tttaagacta tgcgcggtcc agtgcaggcg gttgaccata
     5521 ttgaccttga cctcaaaaac aatgaagcca ttgtgatatt gggcgaatct ggttgcggta
     5581 aaagctcgct ggcgaaggcc atactccgtc tattacccag aaacatcgac aagtattcgg
     5641 gcaaggtttt attgaatggc acagatacca tgcagtactc cgatgaagag tttcgccgaa
     5701 aagtcaggtg gcgttccatt tcgatggttc cacaggcggc tatgaactcg ctgaacccgg
     5761 tgttgaaggt tggcgaccag gttacagaac cctttatcat ccatacaggt gcttcaaaaa
     5821 cagaagcaat tgcgcaagcc aagaagatgt tcgcgcacgt cggtgtgccg aatagcttcc
     5881 tcgatcgcta cgctttcgaa ctcagtggtg gcatgaggca gcgttcagcg attgccatgg
     5941 cgttggtgac aatgcctgat ttgatcatcc tggatgaacc cacatccgca ttggatgttt
     6001 taacccaggc gaatattttc aatgtgttga aaacgctcaa gaaagacctt gagatcagct
     6061 ttatcctcat cacccatgat atcgcaacct caagtgaatt ggctgatcgg gttgcggtga
     6121 tgtacgctgg acagatcgta gaagtctgtg atgcgacccg tttctttgat aaacctttcc
     6181 atccatactc aatcgcgctg atggatagcg tcccacgctt acgccaggtg aaagaaccgg
     6241 acttcatcac cggccaaccg ccatccttga tcaacccgcc aaccgggtgt cgctttaagg
     6301 atcgctgcca caaacgcttt gaaaagtgcg aagaagaacc gcctgcagta acaattgaaa
     6361 atcatattgt taaatgttgg ctttaccaat aaaggtagga gatgtcatgg atcacataat
     6421 tgacaaaaac gagaaggcag aaccgttcct ccaacaagac atccgcgaga gaggtgtcct
     6481 tctctccatc agagatttaa agatttggtt cgaactaaga aaatttggtt ttggtcacgc
     6541 agggtatgtt cacgcggttg atggcgtcag ttttgacctc cacaatggag aatcaatcgc
     6601 tgtcgtgggt gaatcaggat gtggaaagtc caccttgatg aaaggaattc tgggccttca
     6661 ccgccccacg gaaggagaga tctttttcag gggtgagtct ataggcgaaa tgaagtcgaa
     6721 agaaatcaaa cagtatcgtt cgcacatcgg ttacatccag caagacccct atggtgcgat
     6781 ggcgccgttc atgtcggttc aacgtattct gacagaacca ttgagtatca acgggattaa
     6841 ggaccatcag cagattcaag acatggttca caaagcgttg gaagaagtta aagtttttcc
     6901 agttgaagat tacttgaata aatttccgca catgttaagc ggtggccagc aacaacgcat
     6961 cgttattgcc cgttccatga tcttaaaccc caacttcctg gtggcagacg aacctgtttc
     7021 catgctggac gcctccgtca gggttgaaat cctccggttg ctccgcagcc tgcaagaatc
     7081 caggaatctc gcggttatct atatcaccca cgacctttct acggtcagtt acttttcacg
     7141 tcggattttt gtgatgtacg ccggtcaaat cgtagagaaa gcattggttc aagacttgct
     7201 gaacaacgcc ggacacccgt atactcaggc gttgctcacc ggcacctcag accctgatgg
     7261 tgccaacgca cataagctga aagaagttcc accaggtgaa cctcctaacc tgatcaatcc
     7321 gcccaaagga tgccgtttcc acccaaggtg cccaaagatc attagagggc tttgtgaagt
     7381 agaggctccg ccagaatttg aaccagaacc tgagcatagt gttgcctgtt ggctgcttag
     7441 atgatcattg accgcctaaa tcccaggaaa ttgatctggt cagccatcat tttgatggtt
     7501 atgggtgtag caattccgtt tctcatggtc atccacgttc tggacccgct attgagatgg
     7561 aattcaacca tttatttgat cttaaacttc gttgcctatt tcttccaaat actcgggttc
     7621 attcttggaa ttatcggcat cgctatctat actcgccgta acaaacatcg agaatgacac
     7681 ctcattttct acaaatagaa agagcgagga gcaatcccgc tcttttttct tatgtttttc
     7741 atatctgttc ttgtttataa ttggtttaat attgaaaaca ttgattggac ttctatgaac
     7801 gcaatcggta gcaccgcaat caccattctt gaatttatcc tcgccttcgg cgcgctggtg
     7861 ctgttacatg aacttggtca ctttttcgct gcgcgcctga gcaagatcga agtcgaggag
     7921 tttggtctgg gttacccgcc caagatgttc cgcctgttcc gcgccggtgg tacggatttc
     7981 accataaact ggattccctt tggcggtttc tgccgcatga aaggcgagaa cggagaagtg
     8041 tccgaaccag gcagtttttt ggcagccaga ccttccaggc gtttattgac cttgttgggc
     8101 gggccgttga ccaacctgct cgtggggatg ctcatcatct cttttctctt cacccgtatc
     8161 ggctcgcctg atccctcttc ggttatggtc gccgctgtca ctgatgaaac tcctgcagcc
     8221 gccgccggta ttctcccagg tgacctcatc caatcagtaa acaatgtcca aatcaccagc
     8281 atggacgctc tctccaaggt tatttcgtcc cacctcggtc aggaggttga gatcgtgctg
     8341 ctgcgtgaag gtgttgcgct gacgatcaag gtaaccccgc gcgctgaatg gcctgaaaaa
     8401 cagggtcctc tcggtataac gataatgaac ccttcccaaa aagtcagctt cattcaggcg
     8461 ctgccaagag cgtttatcgc cacccttgac cagggttacc agttgttgat ggtcccggtc
     8521 aggcttatca gcgggcagat tgccccttct gaagcgcgca tggtgagcgt aaaagggata
     8581 tacgatatct acgcgcaggt tcaaaccctg gacaagcagg aggctgtgga gaatcccgcg
     8641 atggccgggc tgaacacgct ttactttttc gccatcattt ccatcgccct ggggttcacc
     8701 aatttactgc ccatccccgc tctggatggc ggtcgaatcc tgttcctgtt gccagaactg
     8761 ttcttcaaga aaaagatccc ccccgcgcta gaggggaaag tacacatgat tttttatgcc
     8821 ctgctgctga ctttaatggt cttactggtc atcaacgata tcgttaaccc ggtgatcatt
     8881 cctaaatagg cgaccgatga ataaagaatc aggcgaaccc ataccatttt cttcggttct
     8941 ttcagcatta caaaatgctt cagttccctt cccggcgcgc tatctgcgca gtttctcaga
     9001 cctcccgcct gccaacctga aggtcctgct caactgctgg ccatccatcc ctgccaggcg
     9061 caaggtatct ttattagagg accttgaagc cgtgttggaa gaggacaccc tggtgaattt
     9121 tgataacctg gccagggctt tgctcgccga ccccgacccc catgtgcgca cctgcgccct
     9181 gcgcctgtta tgggagtgcg aagaaaaatc catccttccc atcttggtcg atatttctat
     9241 caaagacgaa gatgaaacga cccgcgagac cgcgatttca ttgctcggca agttcgtact
     9301 gctgggtgaa ctggattctc tgaaagacag cgcgaaagaa gggctgaacc aacacctggt
     9361 gaacatcgtc cgggggaagg acaaaacgcg agtcaaacaa cgcgcgcttg aatcgctggg
     9421 ttattccagt cacccagccg tgcctgacct catccgcaac gcataccaaa cgaacgatct
     9481 gaattgggtc acgtcggcga tttgtgctat gggacgttca gccgatgatg cctggggtcc
     9541 tcaggtagta gccatgctca cctcccctga gcccgaaatc cagtttgaag caattcgcgc
     9601 cgccggcgag ctggaattac gcaccgcacg cgagccactg ctcgcattgc ttgaagagga
     9661 aaccgaagac gaagaaatgc gcctggcgtt gatctgggct ctttcacaaa tcggnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcaga tcagattctt cagccaggta
     9841 tgtctttagg tcaatcaggc gatctctgat gaccgcagcc tgttcaaact gcagctcgct
     9901 ggctgcctgt ttcatttgct tttccagttc aaggatcatg cgtttcagat cactgatcgg
     9961 catgcgtggt ttctcttcgc cggctgagta gcgacctttc gcttcagcga tcactgatga
    10021 agcagaaagg cgctctgtca ggtcgtgaac agccttcacc accgtgaagg gttcaatgcc
    10081 gttggctttg ttgtaatcca tttgcttttt ccgtctgcgg tttgtttcgt caatggcggt
    10141 tttcatcgcg ccggttatcg tatcagcgta catgatgacc ttgccgttca cattacgcgc
    10201 cgcccggccg atcgtctgaa tcaacgcagt tgcggaacgc aaaaatcctt ccttatcggc
    10261 gtctaggatt gccaccagcg aaacctctgg caggtcaagc ccttcacgca gcaggttgat
    10321 tcccacgatc acatcaaaca cacccatgcg caggtccctt aaaatgccaa cccgttccag
    10381 cgtttcaacc tctgaatgga gataattcac ttttaaaccc aactccaaca ggtaatctga
    10441 aagatcctct gccatccgct ttgtcagggt tgttactaaa acgcgctcgc cctgttcaac
    10501 gcgcttcttg atctcgccat acaggtcatc cacctgccct tttgtcggtc tcacttctac
    10561 ctcagggtcg accaatcccg tgggacggat aatttgctca acgatctggt cagaacggct
    10621 caactcaaaa tcagccggcg tggctgaggt gtaaatggta taccccatca ccctctcgaa
    10681 ttccttgaag gttaatgggc ggttatccaa tgctgaaggc aatctgaagc cgtattctac
    10741 cagggttgat ttccgcgatt ggtcgccgtt gtacatgccc ctgatctgcg gaaccgtcat
    10801 gtgtgactcg tccagtacta ataggtactc actgggcaga taatccatca aggtccaggg
    10861 aggagagccc gctgggcgct ggtcaagatg gcgtgaataa ttctcaatcc ccgaacaata
    10921 gccaacctcc ctcagcatct ccaggtcata acggcagcgc tgctcaatgc gctgcgcttc
    10981 cagcaccctg ccgttggctt tgaaataatg gatctggtcg ttcagttctt tttcaatacc
    11041 atccagcgcc actttcaggc gcgcttcgct ggcgatgtaa tgcttggcgg ggtaaatatc
    11101 aatagcagaa ggttccgcaa tgatctcgcc ggtcattggg ttaaactcaa cgattttctc
    11161 tacttcgtca ccgaaaaaag tgattctaac agcctgtttt tcctcgtaag cggggaaaat
    11221 ctccatcgta tctccgcgaa ccctgaaagc ccccggccgc aattcaaggt cattgcgctg
    11281 gtactggctc tcaattagcc gtcgcagcag cgcatttcgt ctaaatattt caccgaccgc
    11341 aaggtgaacc accacattgc tgtattcttc aggattgccg agaccgtaga tgcaggatac
    11401 agaggccaca atgatcacgt ctttccgcga cattaaggca gtagtagccg ccaggcgaag
    11461 acgttcaatt tcatcattga tatccgtctc tttttcaata taaagatcgt ggcgcggaac
    11521 atatgcttcc ggctggtaat aatcatagta agagacaaag tattccactg cgttttgcgg
    11581 gaagaactcg cgaaactcgg cgtaaagctg agcagccagg gtcttgttat gcgccatcac
    11641 cagcgccggc atctgcagct cttggatgac tttggcaata gtgaaagttt taccggtgcc
    11701 agtagctccg agcaacacct ggttacgcat tccctgtcgg atgcctttga taagctgctt
    11761 aaccgcttcc ggttggtcac cggtagcctg gtatggtgcc tgaaggtgaa actgcattct
    11821 cttttcctgg ttttatttac cttgattata gcacatttgt tcttctatgc gtgtctaagt
    11881 gtttcggatg ggcagctcca gtgaaaacac cgcccctcct tcggggctat tcgcgccact
    11941 caagtcaccc cccaataaaa gcgccagttt tttgctgatc gccagtccca gccctgaacc
    12001 tccattttct ctggttcgcg cgcgttcgtg acgatagaac ctttcaaaaa gatggggaag
    12061 cgcggtggat ggtataccgc ttccatcatc ctggacggag ataaccagtt tggcgctttc
    12121 tttcctggtg cttatgagga tgcgccctcc tttttgtcca tagcgcagcg cattggagat
    12181 caggttctgt aaaatttgcg aaacccggtc gacatccgtg tgaatccaca attcttcacc
    12241 atctggacga aatacggtca ccagattggt tccttcgagc gctgcctgtg cctgaaaacc
    12301 agcaaccacc tgttgcacaa ggttcacaat attcagaatt ctcatttcga gacgaacttc
    12361 gcccgcatca gccatcgcta acaatctgag gtcttccacc aggcgtgaga gaaaattggt
    12421 ctgctgtaat gcgatcagaa catttttttg tgttagcggc agcacgccat caagcattcc
    12481 ttcgagctgc gctttttgta cagcgatggg ggttcgtaac tcatgcgccg catccgcagt
    12541 aagcgactgc tttcgctctt cagccagctc caggtttctg gccaactggt tgaaggtttt
    12601 ggcaagcgtg gcaatttcat cgttgccttt gatatccacc cgggtagaaa aatcgcctgn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna agtggttttg gtcagtcaat
    12781 gtcagttctg aggctttacc gtgagcgttt ttcctcccgt gccgttgcga ataagcatcg
    12841 gcaacttccc ccaccatact ccatgtcccc ttgtattcat agtagtcttc cagggcttca
    12901 acaagctcca tcacaccata ctgtcctccc cttgtcagat accggttcac ctggcgctgg
    12961 ctatccaggt tggcaaaaat caccaccccg ccaattgaaa cgatgaccac aactgaaaac
    13021 gccagaatca ttttttgctg gagcgaaaac ttcattattt actcatcctc tcggtagaac
    13081 ttatagccaa ctccaaacac tgtctttata aaagcgggtt ttcttgcatc ctcttcaatt
    13141 tttgctcgta aattttttat atgggcgtcc accgttcttt cgtaaccttc aaagctctgt
    13201 ccttgcgtag cttccaataa ctgaagacgc gaaaacacgc gaccaggatg acgcgcgagt
    13261 gtcaccagta ttgcgaattc agatggcgtc aagtcgattt cttgacctgc ttttttgacc
    13321 aggtgcgcat ctatatcaat ctcaaacggt ggaattaaga aacggtcctc ctccaccctg
    13381 ctgcgattca ctctacgcag aactgtttta acccgcgcga cgacctcccg gggtgagaac
    13441 ggcttggtaa tgtaatcatc agccccgagc tccaagccct ttaaacggtc tatttcttct
    13501 acccgagcag tgaccatgat gatcgggata tctttttccc tcaacacctc ggtggctaca
    13561 tctacaccat ccatcccagg tagatttaag tccaacagga ccaggtccac attctcgctc
    13621 aggcagaggt ccaaacctgt atccccgcgt tgagcagaaa gcacacggta gccttcgcgc
    13681 tcaagatatg attgcaaaat gcgcgccagg gtaacttcgt cttcaattat taatatggtt
    13741 gtcatgaagc gattatagca cccagaacct tcagcaagtt ttcccgccac tcgttctctt
    13801 ccgggctgaa cttcatccag taagcatttt taccaatcct ggtttgataa ccaagcggcg
    13861 caagttcacg ttttaccgcg ccttctggca gttgcggaaa acgcagcacg agttgatctg
    13921 cttcaacgtt aatcgaggtc aacccggctt tcgctgccag tagttttacg cgcaattgga
    13981 agaagaggtc cttaacttgc tgaacaggtt gtccaaaacg gtctcggaat tctgctgcca
    14041 aagcgtccag ttcagagact tcctccaggt gagccagtcg ccggtataac tgcaatcgta
    14101 aagcctgatc cggaacgtaa tcaacaggaa tacccacgct aatcggcaga tccacactga
    14161 cggggatata cccggaggaa ggaattgcgt tttcagattc tccttcattt tgcccctgtt
    14221 caaggcgtag ctcccttact gcctgtgcca gcatcctggt gtagaggtga aaaccaactg
    14281 aagcgatata acccgattga cgggtaccga gcatttcacc cgctccacgc atttcaaggt
    14341 cacgcatggc tatggaatag cccgccccca actggatgtt ttccgcgatt gtttccaggc
    14401 gttcttgccc ttcaggggtt ggggctttct tgcggtgttt gaaaaaataa gcgaaagccc
    14461 gttgcccgcc cctgcctacc ctgcccctca actgatacaa ctgcgccagc ccaaaggcgt
    14521 ctccccggtc tacgatcaaa gtattggcgt tggggatatc caagccagat tcgataatcg
    14581 aagtgcacag taacacgtcc acctcgcctt tggtaaaacg ggtcatcacg cgagaaagct
    14641 cctcttcacg catttggcca tgtgcgatcg agacccgcgc ttctggcact aaactctcaa
    14701 ggtgagcttt catcgcctca attgtttgca cgcggttatg cacaaaaaac acctgcccgc
    14761 cccgcccaat ttcacgtagc accgcctgcc gaacgatccg cggcgaataa ggaccaatat
    14821 gagttacgat gggcaggcgc tctgcggggg gggtattaat ggtggatata tcacgcactc
    14881 cagtgagcgc catatacagg gttcgcggga tcggtgtggc agtaagcgtg agtacatcaa
    14941 cttcggtacg taatttttta aaatgttcct tatgagtaac gccaaaccgc tgctcctcat
    15001 cgatgaccac caaacccagg tctttaaaaa ccacatcgct ggtaagcaga cggtgtgtgc
    15061 caattacaat gtcaacctgc cccagcatga tccttaaaac aatttcttcc tgctcttttg
    15121 gggttcgaaa cctggaaagc atttcaacag aaaccgggta aggagacagc ctctgccgga
    15181 aggtctcaaa atgttgttgt gcaagcacgg tggttggaac aagcatcgct acctgctttc
    15241 cgtccatcac cgctttgaag gccgcccgta atgcgacttc tgtcttgcca tacccaacat
    15301 cgccgcaaag caaacggtcc ataggctttg gcgtttccat gtcttgcttc acagataaaa
    15361 tcgctttcat ttggtcttct gtttcaatgt agggaaagct tgcttccagt tcttgctgcc
    15421 aggcggtatc aggcttgtaa gcgtgcccaa ccgcagattg gcgcttggcg tatagttcta
    15481 acaattcact ggcaacctgc gccaccgctt cgcgtacacc ctgcttggtt tgattccact
    15541 catgtccgtt taaacttgag ggctgaggtg gttcaccacc cgtacctatg tagcggctga
    15601 tccgatctgc ctggtggata ggaacgtaaa tttgatctcc attttcgtac tccaccagca
    15661 ggaattcttt ctccatacca tccagaattc ggctcaccaa cccggcaaac cggccaatgc
    15721 cataatcaac atgcaccacc cagtcaccct tcttaaggtc agcataatct gcttccgggg
    15781 agagataacg cgcggttaac cgcgccctgg gttgaggtcg ttcccatcca aatatttcgc
    15841 catctgtaat gcaatgtagt gttttcccgc tatcaagttc gagatcccag ccttcaccca
    15901 atgcagattc gatgaatttc aaatttcctg aatgagcaca agcgtcgctt tgctcttccc
    15961 agatctcttt taaccgtgaa agttgcctgg taacaatcac catctcatcg ccttcacgac
    16021 aacaggcttc aagatagttg acgaagtgac tgatttgccc cgcaaagcgc ataccgggtg
    16081 aaaatttcga gccaagagaa cccatatctt cattgttcgg gcgtaacagc tccatccagc
    16141 gccggttgga gatcatttca agcacttctg accaggatat atacggaacc gggaaatcag
    16201 caggaatgat cccttctcgc acaacctcag ccctattctt taaagcctgc tcttctattt
    16261 cttcgccaaa actttgaata ttcgaaaggc tatcaaccaa aatcaacgca tccggtggca
    16321 aataatccag gatcgtggat gaaagcggat gaatcatggg aatttggaac tcatcaacaa
    16381 aaccgtcctc acccgaaaac ttcaggtcgc cgtcagggat gaattctctc gcaggggtaa
    16441 tttgtatctg atcgatgtta ctcacagttc gttgtgtggc cgggtcaaaa aatcgtaagg
    16501 tgtcgatctc ctcgccaaaa aactccagtc ttaccgggta tggttcagca ggcgtccaga
    16561 tgtccaggat accccctcgt ttggaaaact gaccgtggtc aacgaccacc tcggtgtttt
    16621 cataccccag gcgcgcccat tcccgcaaca acgtgtctgg attggcatgt ccgttgattt
    16681 ttaatacctt actcgcaata atataatcgc gcctcggcag cgttcgcgtc atcactgccc
    16741 gcagtggggc tataaccaca cagggtttat cgggtttctg gctgcccagt ttaaaataat
    16801 tcaccagtac cgccatcgtt tgcagccggt cccgccgggt caggctgccc caggcggctt
    16861 tttcgtaaaa catgggtgta ggttcaggaa aatacaggcg cgggaagtca ggcagccaaa
    16921 agcgtaattc atcaaacagc atggaggcgc gatctgctct atccgttaat agcaggactg
    16981 gcacttgaag atcgatgcac agggctgcca ctacaggcag cctggctgaa cgcagcaaac
    17041 ccaggctggc tggctgtgtc cccaccttga tagaatcaag gcactgcagg tatgccgcct
    17101 gttggcgtaa tgtgctaagc aatttaatcc ttctctgttg ggttgaagcg attcatggca
    17161 gtctccaacc cttcattcaa ataacactga agcgcctcca ctgcccggtc cagaaatgac
    17221 ccaagcatct ctttttctgc tttcgtgaag gtctgcagca cgtatgccgc tgcgtccatc
    17281 tgaccagggg gtcggccgat gcccatgcgc agtcgggcaa aattttgtgt tcccagttgt
    17341 tcaatgatcg aagtcatgcc cttttgcccg gctgaaccgc cagcagggcg tattcggatg
    17401 gtctggaaag gaaggtccac atcatcatgc ccaacaatta agttctccaa cgaaaccttg
    17461 tagtaccgta ccaatccagc gatcgcctga cccgactgat tcatataggt ttgcggcttg
    17521 gctaagatta ccctgacgcc gttgacgcga cccacgccaa cgatggcttt attctgtact
    17581 ttattcaact tgatattgtg ccgcgctgcg aatgagtcaa tcatcatgaa accgatattg
    17641 tgtcgggttt cgcggtactc tctacccggg tttccaagcc cggcaagcag gtataccgct
    17701 tctgtcgatt ccttaatagc agaggttttt ttgtagaata ttttgagtaa tgacatattc
    17761 cctcttatcg ccgtcgacgg gattgtatcg ctgaatagat ggcaattgaa gacagcgcaa
    17821 cacagacagc aagaatggca ataatgatcg ttcgttgaat ttcctgccgt tggatggaag
    17881 ccgggttgac ctttttttca atcacggtcg aaacaggtgc tctgatctca accgctgctg
    17941 atcgtttctc cgtctgcgta tttacaccca tttcattctg acccggagca cgagagtagt
    18001 tggaaacctg tatgttctca accaccacct catttcgacg atcccccacc tttgttacca
    18061 caagtttgat atggtagacg ccgtcggtga tggtggttgt atcccaggca gccagaacat
    18121 catcctgaac cactttgtcc agtcgcttga ttagaaacca ggtttcttta cccgtttgcg
    18181 catacgcgta cagaacttct gctgataaaa atccttcttc cggtacactg ccccggattt
    18241 ctacaatccc ttctaccacc tcgccggttg tgggcgcagt gatatacaat ccgggtgtaa
    18301 ttgtcatccc caagag
//
LOCUS       DAOSPM010000035        18247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_3067_length_18247_cov_13.220921, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000035 DAOSPM010000000
VERSION     DAOSPM010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18247
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3067_length_18247_cov_13.220921"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..625
                     /gene="gcvH"
                     /locus_tag="PLW25_04645"
     CDS             233..625
                     /gene="gcvH"
                     /locus_tag="PLW25_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOS53811.1"
                     /translation="MNTPKNLKYASSDEWVLIEGDVATLGVSDYAQDQLSDVVFFETT
                     VAVGDTLEKNAIIATIESVKAAADVKSSVSGKVIEINEALADTPELINSDPYGAAWMI
                     KVELANPQEAEVLMDAEAYAAFCETRGH"
     gene            625..1977
                     /gene="gcvPA"
                     /locus_tag="PLW25_04650"
     CDS             625..1977
                     /gene="gcvPA"
                     /locus_tag="PLW25_04650"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOS53812.1"
                     /translation="MFIPHTEVEKKEMLKGIGVKDVKDLFTDVPEKFRYPKINLPPGV
                     TEMEVFAELNDYAAANEPVGEMVCFLGAGAYHHYVPAAVDALLRRGEFYTAYTPYQPE
                     ISQGTLQAIFEFQTLIANLTGMDVCNASHYDGATATAEAGVMAYHHFRGKRKKIILSP
                     AVNPQYQEVLCSYLCGFEDVDIHSGKLHGGLSDQPSDLGALIDDQTALVVVQYPDFLG
                     RIQDFSSLVYAAHQSGALVAFVVNPIALGLLKTPGELGADIVVGEGQPLGIPLSFGGP
                     YLGFFATRKEYVRKLSGRLVGETTDAQGRRGYVLTLTAREQHIRREKATSNICSNQGL
                     MALAATIYMSLVGKHGMRQVADLCYQKAHYAASEIAKIPGFQLVSKEPFFHEFVVKCP
                     SGIDEINRDLLDMGFIGGYDLGQTHPELTNQMLVAVTEMNSKEDIDAFAAALQEVTHG
                     "
     gene            1970..3433
                     /gene="gcvPB"
                     /locus_tag="PLW25_04655"
     CDS             1970..3433
                     /gene="gcvPB"
                     /locus_tag="PLW25_04655"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOS53813.1"
                     /translation="MVEPTIYEISSNGRVGFRFPESDVPRVDLPDRLKRSDLPFPEVS
                     EVDVVRHFTHLSTLNYSIDKGFYPLGSCTMKYNPKVNEAAARIPGFSATHPLQPIETV
                     QGNLALMYGLQEFLKEISGFAAVTLQPSAGAQGEFVGVKIIKAYHQSRADEKRTRILV
                     PDSAHGTNPATSSMTGYEVVGVPSDARGNVDLASLRDICDETIAGLMLTNPNTLGLFD
                     ENVLEVIEIVHQVGGLVYGDGANLNALLGIARPGDLGFDVMHFNLHKTFSTPHGGGGP
                     GSGPVAVTQKLADFLPAPIIGIVEEATEDVPPLYGFITPPKTIGRVKAFHGQFGVLVR
                     AYTYIRMHGPDGLRKIAEHAVLNSNYLLSLLRHRYTLPYDRTYMHEFVLEGVWADAPD
                     VQALDIAKRLMDYNFHPPTNYFPLIVREALMIEPTETESKQELDAFATAMLKIADEAH
                     STPALLKEAPHNTPIGRLDEVKAAKELVLCCWLPEQE"
     gene            complement(3482..6211)
                     /gene="alaS"
                     /locus_tag="PLW25_04660"
     CDS             complement(3482..6211)
                     /gene="alaS"
                     /locus_tag="PLW25_04660"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOS53814.1"
                     /translation="MKQMTGAEIRQGFIDFFKQKGHTYVPSSSLVPGGDQTLLFTNAG
                     MVQFKDVFLGIDKRDYTRAVNSQKCMRVAGKHNDLDDVGRDDSHHTFFEMLGNWSFGD
                     YYKKEAITWAWELLTGVWGLHPEHLYATVFKDDKGNIPADVEAAEIWRQQPGMSPEHV
                     LSFGRKENFWEMADTGPCGPCSEIHIDRGESFCNKKEVPGHICKVNGDCPRFLEIWNL
                     VFIQYNRLDQDTFEPLPARHVDTGMGFERIVAILQNVNSNYRTDLFTPLLDKIRELTG
                     MSVAAMNEDFTPYRVIADHTRAASFLIADGVVPGNIGRNYICRMIIRRAARFASKLGL
                     TEPFMAQIASIVIDQYRAAYPELEKNRKTILDNLTREELRFQRTVEGGVSQLEELMDE
                     LKKSGKKVLDGSKAFELYATHGLPLEITRDIAREVGLDVDEPGFKKAMEEHRLHSGAG
                     KAIGTMGGNEVELYRKVYVELVESGKIPAGGVSYDPYHALEIETQIVGLFSEGEVLSE
                     APRGSEVEVILPKTGFYIEAGGQVGDTGTLTALDGSWEIKVEAVRRPAAGIIIHIGEV
                     VHGTVRNGDKVLMKVDRQRRMDIMRNHTATHLLHVQLHKVLGNHARQAGSLVAPDRLR
                     FDFTHPEALTQEQLDEIESGVNREILASHALVIKEKTLQDAMAEGAMALFGEKYGEKV
                     RTVTIDEENPISYELCGGTHVENTGDIGTFLILSEGSAAAGIRRIEGVTGRGAYEMIQ
                     ARNKILRRAARLTNSSMEEVVAKINSLQDEIESLQVELGRVRQEWVKADFEGKLDNIL
                     YIAGIPVLSVLLKGADIETMRQMADIFRHKHPSGIVAIGSIINEKPMIICSVTEDLVK
                     RGLNAGELARQAAAIVGGSGGGRPVMAQAGGKDPARLPEALSSVIETLKRKLT"
     gene            complement(6328..8853)
                     /locus_tag="PLW25_04665"
     CDS             complement(6328..8853)
                     /locus_tag="PLW25_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOS53815.1"
                     /translation="MLQILNAGLATGSYRFTRQAALAWMTTYPGDLEINLLYAHALVK
                     EMRFSHAVPVIQKILRADPEYLDAAVLGEEVFARSDPAFLPVVSGTIKALGGTPVHGE
                     KVPEWGLSLNRIRQLIQRRQNEEARQQLMEIINITEQVELACYYHLLLTKRLGDRDTL
                     ITLARLYHSRWPENIQITLLLAGALLEGGETDEAVQLLHYCAANDPSAQVITRLWGDK
                     HSFKPLYPVNMQIDFDLPIPAEVSGKLGLNQLGAGGETVPGVPPTSPTATEVTSFDGY
                     RVLPTKDEFYPEHPKVNQESASSKKQFIAEVEATLKKVALDISQPSLSRTDERFPAYV
                     ILTMKSGLEKQYGKQSARLVIEELKNLAKTVEKNAGWTSIVFLPDDLQSCGKYNITPV
                     DRLDPWKIKLSLVDLDKALVRSGERIGAVLIVGGDEVVPFHRLPNPTDDSDESVPSDS
                     PYGALDTNYFVTDWPVGRLPGEYGADVGLLMEQMRNLMGYHGSPATSTSLIDSILNLL
                     FFWNKGLADRHFNLGYTASVWRRSSLVAFRPIGEGKYLYLSPNGKNASFDVRKLADAQ
                     VGYFNVHGVEDAGEWYGQKDPTENDSGPDFPVALRPMDIQRITRTPRIVFCEACYGGH
                     ILEKAEDESIALTMLGKGVLAMVGSTTISYGSVTTPLIGADLIGYLVLKNLRDGLSIG
                     ASMLKAKAEFVREMNRRQGYLDGEDQKTLISFVLYGDPLVAYDQNREMKKMMPRDLAH
                     PVIKTISDSLSLDQQTEMLAPKMIAQAKSIARDYLPGIENAEVRIKNQQYNQRNNKKY
                     TQPSGKVVVSFSKQLKQANRVHQQYARVTMDKHGKIVKIAVSR"
     gene            complement(8907..>10048)
                     /locus_tag="PLW25_04670"
     CDS             complement(8907..>10048)
                     /locus_tag="PLW25_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS53816.1"
                     /translation="ESLAQALELWPNEYRWHVKFAELWQRLGNAERPVESLRLANLYA
                     QDEPEIQEKLGKALLRNEKPDEALKYLLAAVEKQPGHPDLWLSISEAHQQSGDLDLAL
                     EAAERAVQVDPYAVKARLQAGKVRWKRGELEKALDQVNLAISLNEDDASSYVLMAQLL
                     NDQGDKSKALQMLEKATQSKNADVQVLVEHASLLKDIKGASAARDLIASFSDRYPENP
                     ELLRLLAEAEDQCGNIKKAEMVAKKALAIQPEEVDLQMLLGKIQEKVGNLDQAVSYFS
                     QAIALEPRRKDAYLLLSQVYLKQRDHLKARQALEQGITNNPGELDLYLACAALLKEAK
                     DYQAAEQMLRKASSLEPRNVNIHRQLGAVLALNLVHQSQEVSSQP"
     assembly_gap    10049..10148
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10149..14377)
                     /locus_tag="PLW25_04675"
     CDS             complement(<10149..14377)
                     /locus_tag="PLW25_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS53817.1"
                     /translation="MRYLDLITTLNHLTQKQEAVVQFKALRQDPFIWEEFQQLTEDSP
                     VITSLTRQGMPLNPGTLTVLLYDPEFDFSTLNARMTSREKLEKVMFSYEEYIQGDQPP
                     ATLAQVGGLALSLLEKRKTAASWAEIIQEIVTRMKINHGDRFLLLWGTVFVVVTNLIE
                     DKDSCFRDLTIFQQAESSIEIFTHLVLCLPWTEQEKVEVLRRYLYPLSPQIQVAALKQ
                     LKKTSDGLIYAQVASKILEKYQPPDSAKKSTGDYWKNPVSSMHYAFQCQSVADIAQLA
                     GNTAVAADLNEKALEVISALLKMSKVKKADIHFDAAAESIPLGELFSTEELADPEILA
                     ELAYTSAAGDLQLSGESATAKIMQQAKKMTKAGNVDLARAELRENLKLLSESEFERLL
                     VDGPEHIQSWEPVTLLTLLVESGAYDEAERLGKVLIARNPSSEPVIAALAAAAEGKGD
                     HAAALGYLETLNALDPGSIGVMRKLADAQIRGNAYDAAYQTYRELIDQADLPEEKDLN
                     RFGEIALMVGKADEALTASAEILGRNPDSARGLTLAGMAHHRKGQDAAAIDELRRAIA
                     LAEGEVKPWIELAEIAWTGGDHSSSLTTLKEGMAANPGNAELQRVYAQRLMDEGLVSE
                     AFPYLLELSVKGHSAEVDLQLVAAMKQLGMENIDETIAGFVNRYPGDHRFIGEFGSRL
                     VWKGEQEKGLGYLKSIADHLKEDTVWSMAYLEGVLQPDYVHLVSPARLAKSELIFTKE
                     LLDDLLIREPDNLHAKMLKAEWLLQSGEYTAARKLFDAIMDESQGGRELPSARLFAGL
                     AQSAAQNGEHEIAMAALEQAISLQPSWHGLLRLKAEFLNLSGDMNEAVKQAESALEVA
                     PDHPENHVWLIEFITALGRTGNLEKHIKSAVTQYPDHLGLRLLQAQNRIGINTDEESL
                     EIEQCLLALIDKTDDAGALIKAAMIFSNLGNPEKTISCLEKAAAQGSSQAQLSLAGLY
                     RMSGELEKALSVLESVQPTTGMTELFKRETAFTHSGEGTWLDIDLDEHLRPLDIMIDE
                     VFLPMDWKELLDSSRPEIVLKSRIAMTAGSPEELMNDVRDWINAEPENLEARLYGIET
                     ALACCSQEDYQHFLDEIRLDPKHPLVGYLNLLKSERELDDGTLVRDEPVEGDIFDSVS
                     VGEPEKISFIRLLANEGNYAAAETSLEMAINVFSEVQAFPFIVKIGIFRNLAKSAAAL
                     ERWPEALQFGARATGLVPSHQVNRLMHLKHQALAQEMANRAESLGVERHCALSEQKEP
                     TPDNAEMERITNDAVKEETRRWVLRYQLAKEPTRENIRELALLTPTPEDVAALASALR
                     RIGQSRTALQVVKKFAEHPLVMFEQCVCTAENDVPKALEILERSLRIQPQQPLSLRLR
                     ADLLERTGNLTAAAESLAQALELWPNEYRWHV"
     gene            complement(14422..14742)
                     /locus_tag="PLW25_04680"
     CDS             complement(14422..14742)
                     /locus_tag="PLW25_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53818.1"
                     /translation="MFSMTVSDFLLTMASVLLFCGVVVLGIGVFTLIGKLNGKEIRTI
                     ADQTTKLAQKGIADEISGLVGNARSLIEALDQMVKTTAGIGIFLVILGLLLIAAAYLL
                     VTKI"
     gene            complement(14805..15974)
                     /locus_tag="PLW25_04685"
     CDS             complement(14805..15974)
                     /locus_tag="PLW25_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOS53819.1"
                     /translation="MSLSVLVVEDEDNARSFVSTFLKKKGYEVFEASTLDEARERVKK
                     GDGDVVLLDVQLHGEYGLNLLYETAMMPARPPIIVITGFGSIDMAVDAMKNGATDFLQ
                     KPLDMSLLEKSLQRAREIVAMRRELEHYREAQMQKGNFVIGTNEVVKNTVQQALVASE
                     KQVSVLITGETGTGKDVIAKYIHSIGPRANKPFISINCAAIQSTMLESELFGHEAGAF
                     TSADRKKPGLMEIADGGVLFLNEIASMPVDIQAKLLTAIETHTFRRVGGTALIKVDVQ
                     IIAASNRNLKEMIKKGDFREDLYYRLKVVDLDMPPLRERKEDIPELVGFFIRENNARQ
                     GMNVVEVSPKAMEVMQQYSWPGNIRELSNAIERAMLFCDGMTINLADLPKDLTST"
     gene            complement(15978..17918)
                     /locus_tag="PLW25_04690"
     CDS             complement(15978..17918)
                     /locus_tag="PLW25_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS53820.1"
                     /translation="MRAISGRFSSVLKGKNEPGLEEITALLNFDETPAALVENETRSI
                     LFINSRLMRVVSYSHRDVFRKNVGFLFPGLDLSTSFSGEIRETQIKRRDLTPLKARVR
                     FDFIDKGGKWTVIRVLPETLDEQERKLHLEDTYRLLTRFSRLADTGDIAEKIYQGVDI
                     ICEILGCETVCLYQADSSLPQLKRVAVSGNVEDFPRKLPSTDLMKLEKPTIWVTGSRI
                     MTELHREARVKNIEYVASAPMLEGGGAFGLLVAAGKGGIPVSLSLDGMEMLASMLLAL
                     FQQQVLVNNLNERIERSMYSVNLKNQVFESMNEGVIILDNLLKIEQINPAAEFMLGYA
                     AWEVKGQSYENILIGCDRLLPALVDAQKGKETLNVGKAVLNRRNGHAFPVLMKVIPVH
                     LGESVSAVEILMMDISEHEESKAVTQQLEHRAVLGDYTAAFAHDVRNPINNISTGLQL
                     LAATLDENDPSQDVINRMQGDCMRLDHLMESFLAFSRPMELKYEPFEVEPFLKRIIDR
                     WLPRMSRVNVKPILHVEEQLPKIYGDPRSLENVFTNLISNALEAMTDNGDTLAIKAVK
                     VLEVDHRPLVEISVSDNGPGIPQDIKTRIFEPFVTTRKMGTGLGLAITKQIVNAHKGS
                     IKVESFPGGTVFTVQLPADIGE"
     gene            complement(17915..>18247)
                     /locus_tag="PLW25_04695"
     CDS             complement(17915..>18247)
                     /locus_tag="PLW25_04695"
                     /inference="COORDINATES: protein motif:HMM:NF016439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53821.1"
                     /translation="DKYLKPVTRAQLSKELEVHESTISRAVANKSVQLPNKRIVPLAS
                     FFDRSLNVRSVLKEIIEKEPKTYSDSELVEMLTERGFQVARRTVAKYRAIEGILPAHL
                     RRVTIKRK"
BASE COUNT         4244 a         4921 c         4178 g         4804 t
ORIGIN      
        1 ggtttaatca tcgcttcgct tatccgtttt tcaggtttta ttgctttttt tccgctaaga
       61 acgctcatcg gaagaaataa aaaagggtct accaacatca gatgattctt gacttttttc
      121 caactgcttt atagaatgag attgactgcg gcctaaaatt cgcagtgaaa tggggttatt
      181 tcctcatcaa tcgacctgcg taataccaac tactttcata tcaggaggta agatgaacac
      241 accgaaaaac cttaagtacg cgagttcaga tgaatgggta ctcatcgaag gggatgttgc
      301 tactctggga gtttctgatt acgctcagga ccagctctca gacgtggtgt tttttgaaac
      361 cacagttgcg gtgggtgata ccctggagaa aaatgccatc atcgcaacca ttgaatctgt
      421 gaaagcagct gcggatgtca aatcatccgt aagcggaaaa gttatcgaaa tcaatgaagc
      481 cctggctgat acacccgaac tgatcaattc agatccttat ggcgccgcct ggatgatcaa
      541 agtggaactt gcaaatccac aggaggctga ggttcttatg gacgcagaag cctatgcggc
      601 tttctgcgag acaagaggtc attaatgttc atccctcata ccgaggttga aaagaaagag
      661 atgcttaaag gcatcggtgt gaaggacgtc aaagacctgt tcactgatgt cccagaaaaa
      721 ttccgttacc caaaaatcaa ccttcctcct ggtgtaaccg aaatggaggt ctttgcggaa
      781 ttaaatgact acgccgcagc caatgaacca gtaggtgaga tggtgtgttt tctcggagca
      841 ggcgcatatc accactatgt accagcggcg gttgatgcgt tactccgacg cggcgagttt
      901 tatactgcgt atacacccta ccagcctgag atctcacagg gaaccttgca ggcgatcttc
      961 gagtttcaaa cgcttatcgc caacctcacg ggtatggatg tgtgtaatgc ctcgcattat
     1021 gatggcgcaa ccgctaccgc cgaagctgga gtgatggctt atcaccattt tcgaggtaag
     1081 cgcaaaaaaa tcatcctgtc accagcggtc aacccgcaat atcaggaagt actctgttcg
     1141 tatctttgcg gattcgaaga cgtcgatatc cattctggca aactgcatgg tggcctttcc
     1201 gaccaacctt ccgatctggg tgcgctcatc gatgaccaaa cagctttagt ggtcgttcaa
     1261 taccccgact ttttagggcg gattcaggac ttttcctcct tggtgtatgc cgctcaccaa
     1321 tctggcgcgc tggtggcatt tgtggtcaac ccaattgcgc tgggtttgct caaaacaccc
     1381 ggcgagctgg gagctgatat tgtcgtcgga gaaggtcaac ctttaggcat acctctatct
     1441 tttggcggtc catacctggg tttcttcgcc acgcgcaaag agtatgttcg aaaactttca
     1501 ggtcgcctgg tcggtgagac gactgatgcc cagggaaggc gtggttatgt tctcacctta
     1561 accgcaagag agcagcatat caggcgagaa aaagcaacct ctaatatctg ttccaaccag
     1621 ggcttaatgg ccctcgcggc aactatttac atgagcctgg tcggtaaaca tggaatgcga
     1681 caggtagccg acctgtgtta ccaaaaagcc cactacgccg cgtctgaaat cgcaaaaatc
     1741 cccgggtttc aacttgtgtc aaaggaacca tttttccatg agtttgtggt taaatgcccg
     1801 tctggcatcg atgagatcaa ccgtgattta ttggacatgg gttttatcgg tggctatgac
     1861 ctcggccaaa cgcatcctga attgaccaac caaatgcttg tggcggtaac cgaaatgaac
     1921 tcaaaagagg atatcgatgc ctttgcagcc gcattacagg aggtcaccca tggttgagcc
     1981 cacgatctac gaaatttctt ccaacggcag ggtgggtttt cgctttccag aatcggacgt
     2041 acctcgtgtt gacctgcctg acaggctgaa acggtctgac ctgccctttc ctgaagtatc
     2101 cgaagtggat gtggtgcgtc atttcactca cctctcgacc ttgaattaca gtattgataa
     2161 agggttctat cctctcgggt cttgcaccat gaaatacaac ccaaaggtga atgaggccgc
     2221 ggcacgcatc cctggtttta gcgcgacgca cccgttgcaa ccgatcgaga cagttcaagg
     2281 caacctggcg ttgatgtacg gcctgcagga attcttaaaa gaaattagcg gtttcgctgc
     2341 cgtcactctg cagccatcag ctggtgcgca aggcgaattt gtgggggtta agattatcaa
     2401 agcctatcac caatcccggg ctgatgaaaa acgcacccgc atccttgttc ctgattccgc
     2461 ccacggcaca aacccagcca cctcctcgat gacgggttac gaagtggtcg gtgtgccctc
     2521 cgacgctcgc ggcaatgtcg atctcgccag cctgagagac atctgtgatg aaaccatcgc
     2581 ggggctgatg ttgaccaacc caaacacatt ggggttattt gacgaaaatg tgcttgaggt
     2641 gatcgagatc gtgcaccagg tgggcggcct ggtttatgga gacggcgcta acctgaacgc
     2701 tcttttaggg attgcccgcc cgggagacct gggctttgac gtgatgcatt tcaacctgca
     2761 taaaaccttt tccactccgc atgggggcgg cggtccgggc tctggcccgg tggccgttac
     2821 gcaaaaactg gctgattttc tccccgcgcc tatcatcggt attgtcgaag aagccacgga
     2881 agatgtccct cctctatacg gcttcatcac tccccccaaa acaatcgggc gtgtaaaagc
     2941 ctttcatggt cagtttggtg tgctggtacg ggcgtacacc tatatacgca tgcacggacc
     3001 agatgggttg cggaagatcg ctgaacacgc ggttttaaac tcaaactacc tgctttcact
     3061 cctgcgccac aggtacacac tgccctatga ccggacatat atgcatgaat ttgtgctcga
     3121 aggtgtttgg gcagacgctc ctgacgttca agcgttggat atcgccaagc gtctgatgga
     3181 ctataacttc caccctccca caaattattt ccctctcatc gtccgtgaag ccttgatgat
     3241 cgaaccaacc gaaactgaaa gcaaacaaga actggatgcc ttcgccactg ccatgctaaa
     3301 aatcgcggat gaggcccatt caacaccggc tctcctgaag gaggctccgc acaatacacc
     3361 aattggccgc cttgacgagg taaaagccgc caaagaactc gttctctgct gctggttacc
     3421 agaacaagaa taagtgagaa taagccaaaa aaagaactcc cttacaggga gttcttttgg
     3481 tttaagtcag ttttcgtttg agcgtttcaa tcacactgga aagcgcttcc ggcagcctgg
     3541 cgggatcttt tccgcctgcc tgcgccatta ccggacgccc tccgccactg ccgcccacaa
     3601 tcgccgctgc ctgcctggcg agttcgccag cattgagccc gcgctttacc aggtcttcag
     3661 tgaccgagca tatgatcatt ggtttttcgt taatgatcga cccgatggcc acgattccag
     3721 aagggtgctt atgacgaaag atgtcagcca tttgccgcat cgtctcaatg tccgctccct
     3781 tcaacaatac agagagcact ggtatgccag caatgtagag aatgttgtcc agtttgcctt
     3841 caaaatccgc tttgacccat tcttggcgta cgcgccccaa ctcaacctgt aaagactcga
     3901 tttcatcctg tagagaattg attttcgcaa ccacttcttc catagacgaa ttggtcaaac
     3961 gcgccgcccg gcgcagtatc ttattgcgcg cctgtatcat ctcatatgcg ccccgcccgg
     4021 ttactccttc aatgcgtcga atcccggctg ccgcgctgcc ctcactgaga atcaagaagg
     4081 taccaatatc tccagtattt tcaacgtgcg taccgccgca caactcgtat gaaatggggt
     4141 tttcttcatc gatggttaca gtgcggactt tttcgccata cttctcgcca aataaggcca
     4201 tcgctccttc agccatcgcg tcctgtaatg ttttctcttt gatcactaat gcatgtgaag
     4261 cgagtatttc acggttaact ccgctttcaa tttcatccaa ctgctcctgg gttaaggctt
     4321 cagggtgagt aaaatcaaac cgcaggcgat ctggagcgac caatgagccc gcctgccgag
     4381 cgtggttgcc taacaccttg tgcaattgca catgcagtaa gtgagtggcg gtatggttgc
     4441 gcataatatc cattcgccgt tgtcggtcga ctttcatgag aaccttgtca ccgtttctga
     4501 cagttccgtg aaccacttca ccgatatgga tgatgatgcc cgcagctggt ctgcgtactg
     4561 cctccacctt gatttcccaa ctgccatcca gtgccgtaag ggtgcctgta tcgcccactt
     4621 gcccgccggc ttcaatataa aaccctgtct tgggcaagat gacttctact tcactaccgc
     4681 ggggtgcttc agataatact tctccttcag aaaacaggcc cacaatctgt gtttcaattt
     4741 caagcgcgtg ataagggtcg tacgacacgc cgcctgcggg gatttttcct gattcaacca
     4801 attccacata aacttttcga tagagttcaa cctcattgcc acccattgtg cctattgcct
     4861 tacccgcgcc tgagtgaagt ctgtgctctt ccatggcttt tttaaacccg ggttcatcca
     4921 cgtccaggcc aacttcgcgc gcgatatccc tggtaatttc cagcggcagc ccatgagtgg
     4981 cgtaaagttc aaatgccttg ctgccatcca ataccttctt cccgcttttt ttgagttcgt
     5041 ccatcagctc ttctaattgg ctgacgccgc cttctactgt gcgctggaaa cgcagttctt
     5101 ctcgtgtaag gttgtccagt atcgttttcc gatttttctc cagctcggga tacgccgccc
     5161 ggtattgatc aataacaata ctggcaattt gcgccataaa cggctcggtt aaacccagct
     5221 tggacgcgaa acgcgccgcg cggcgaataa tcatgcggca aatgtaattt ctgccgatat
     5281 ttcccggaac gactccatcg gcgatcaaaa aagaggccgc gcgcgtatgg tccgcaatca
     5341 cccggtatgg cgtgaaatct tcgttcatcg ctgccacgct catccctgta agctcgcgta
     5401 tcttatccaa taagggtgtg aaaaggtctg ttcggtaatt ggagtttaca ttctgtagaa
     5461 tcgctacaat acgttcaaac cccataccgg tatccacgtg cctggcaggc aggggttcaa
     5521 atgtgtcttg atcaaggcga ttgtattgaa taaagaccag gttccatatt tccaggaagc
     5581 gagggcaatc tccattaacc ttgcaaatgt gcccggggac ctctttctta ttacaaaagg
     5641 actctcctct atcgatgtga atctcgctgc aaggcccgca aggacctgta tccgccatct
     5701 cccagaaatt ttctttacgt ccaaaagaaa gtacatgctc agggctcatt ccgggttgct
     5761 gccgccagat ttccgcggcc tctacgtcag ccggaatatt ccccttgtcg tctttaaata
     5821 ctgtggcata taaatgctca ggatgaagtc cccaaacgcc tgtcaacagc tcccaggccc
     5881 aggttattgc ctcttttttg taataatcac caaacgacca gttaccaagc atctcaaaaa
     5941 aggtgtgatg cgaatcatca cgcccgacat cgtccaggtc attatgtttt ccggctacac
     6001 gcatacattt ttgagaattg accgcccttg tgtaatctcg cttgtcaatg ccgaggaaca
     6061 cgtctttaaa ctgcaccatt ccagcgttgg taaaaagaag ggtttggtct cctccaggca
     6121 ccaaagagga agaaggcaca taggtatgcc ctttttgctt aaagaaatca ataaaaccct
     6181 gcctgatttc cgctccagtc atttgtttca tatccatttc acctgttctt tcctcaagta
     6241 tatgcgcatg aattatattc gatatttctc ccggttaaca aaccaccgcg gaccttgatg
     6301 atccgcggtg tagactgtgc ggctcactta ccgcgatacg gcgatcttga cgatttttcc
     6361 atgtttatcc atggtaaccc tggcatattg ttgatgtacg cgattcgctt gcttcaactg
     6421 tttgctaaaa ctcaccacca ccttcccgga aggttgggtg tatttcttgt tgtttcgctg
     6481 gttatactgt tgattcttaa tccgcacctc ggcattttcg atgcctggca ggtaatctcg
     6541 ggcgattgat tttgcctgcg cgatcatttt cggtgccagc atctcagttt gctgatcaag
     6601 gctcaaagaa tcgctgatgg tcttgatcac cgggtgagcc aggtctcgcg gcatcatctt
     6661 tttcatctcg cgattctggt cataagcgac caaagggtcg ccataaagca caaatgaaat
     6721 gagcgtcttc tggtcttctc catccagata accttgccgt cggttcattt cccgaacaaa
     6781 ttcggctttc gccttcaaca tggaagcacc gattgacagc ccatcacgca gattcttcaa
     6841 aaccaggtaa ccgatcaaat ctgccccaat aaggggtgtg gtcacagagc cataggaaat
     6901 cgtcgttgag ccaaccatcg ccaaaacgcc cttgccgagc atcgtcaggg caatggattc
     6961 atcctccgct ttttcaagga tatgccctcc gtagcaggct tcacaaaaga caatacgagg
     7021 tgtccgcgtg atcctttgga tgtccattgg tcgcaacgcc accggaaagt caggtccgct
     7081 gtcattttcg gtggggtcct tttgcccgta ccattctccg gcatcttcca caccatgcac
     7141 attaaagtaa ccaacctggg catcagccaa ttttctaaca tcaaagctgg cattctttcc
     7201 atttggagac aggtaaagat acttgccttc accaatgggt ctgaacgcaa ccagagaaga
     7261 acgcctccac acagatgcgg tgtatcctag gttgaaatgc cgatctgcta atcctttatt
     7321 ccagaaaaat aggaggttta gaatactatc aataagagaa gttgaagtgg cggggcttcc
     7381 atggtaaccc atcaaattcc tcatttgttc catcagcaag cccacatcag cgccatattc
     7441 acccggaagc cttcccacgg gccaatccgt cacaaagtag ttggtatcca gtgcgccata
     7501 ggggctgtca gaaggcactg actcatctga atcatcggtc gggttgggca ggcgatgaaa
     7561 aggcactacc tcgtcacccc ctacaattaa aaccgcgcca atcctttcac cactcctgac
     7621 aagtgcctta tccaggtcaa ccagcgagag cttaattttc cagggatcca acctatcgac
     7681 cggggtgata ttgtatttac cgcagctttg caaatcatca ggcaggaaca caatcgacgt
     7741 ccagccggcg ttcttctcaa cggttttcgc cagattcttc agttcttcaa tgaccaatct
     7801 ggcgctctgt tttccatatt gcttttccag gccgcttttc atggtgagaa tcacgtacgc
     7861 cgggaaccgt tcatccgttc ggctgaggga tggctgagaa atatccaggg caaccttttt
     7921 taaggtcgcc tcaacttcag caatgaattg tttttttgaa ctggccgatt cttggtttac
     7981 tttggggtgt tccggataaa actcgtcttt ggtgggcaga accctgtacc catcaaatga
     8041 tgtaacctct gtcgctgtcg ggctggttgg tggtacacca ggaactgttt caccgccggc
     8101 gccaagctga tttaacccaa gtttgccaga aacttccgcc ggtataggca aatcaaaatc
     8161 aatctgcata ttgaccgggt acagcggttt gaatgaatgc ttatcccccc acaacctggt
     8221 gataacctgc gctgaagggt cattggccgc gcagtaatgc aacaactgca ctgcttcatc
     8281 ggtttcgccg ccttcaagca gtgcgcccgc cagcagcaag gttatctgga tattctcagg
     8341 ccagcgtgag tggtaaagcc ttgcgagggt aatcagcgta tcccggtcgc caagcctttt
     8401 cgtgagcagt aaatggtagt aacaagcaag ttccacttgc tctgttatgt tgatgatctc
     8461 catcaattgt tggcgtgctt cttcgttctg tcttcgttgg atcagctgcc tgatgcggtt
     8521 caggctcaat ccccactccg gaactttctc tccgtgtact ggggtaccgc cgagcgcttt
     8581 gatcgtgcct gaaaccacag gtaaaaaggc ggggtcgctg cgggcaaaga cttcctcacc
     8641 caatacagcc gcatccaggt attcgggatc tgcacgcagt attttttgta tcaccggcac
     8701 agcgtgagaa aaccgcatct ctttgaccag cgcatgcgcg taaagcaggt tgatttccag
     8761 gtctccaggg taagtggtca tccaggcgag cgcagcctgc cgggtaaagc gatatgagcc
     8821 ggtagccaaa cccgcgttca atatttgcag cattcgcttt cgatcaacag gcatcgtttt
     8881 tttcgctttc gattgcattt gagggatcat ggctgtgaac tcacttcctg tgattgatgc
     8941 accaggttca atgcaagaac cgcgcccaat tggcgatgaa tattcacatt acgcggttcc
     9001 agtgaagagg ctttgcgcag catctgctca gctgcctggt agtccttcgc ttctttcaat
     9061 aaagcagcgc aagcaaggta aaggtccaac tcacctggat tgttcgtaat tccttgctcc
     9121 aacgcttggc gcgcttttaa atgatcgcgt tgcttcaggt acacctggct gagaagcagg
     9181 tacgcatcct ttctcctggg ttcaagcgca attgcctggc tgaaatagct caccgcctgg
     9241 tcaaggttgc ccaccttctc ttgaattttt cccagaagca tctgtaaatc aacttcttca
     9301 ggttggatag cgagcgcttt tttggctacc atttctgctt tcttaatatt gccgcattgg
     9361 tcttccgctt cagccagtaa gcgaagtaat tctgggttct cagggtacct atcactgaaa
     9421 gacgcgatga gatcgcgcgc tgctgacgcg cctttgatat cttttaacaa actggcgtgt
     9481 tcaaccagaa cctgcacatc ggcatttttg ctttgagtgg ctttctccaa catttgcagc
     9541 gctttgctct tgtcaccctg atcgttcagt aattgcgcca tcaatacata gctgctggcg
     9601 tcatcctcgt taagtgatat cgccaggttc acctggtcaa gcgctttctc gagttctccc
     9661 cttttccagc gcaccttgcc ggcttgtaat ctggctttaa ccgcgtatgg atcgacctgc
     9721 accgctcttt ctgccgcttc cagcgctaaa tccaggtctc cggactgttg atgcgcttct
     9781 gaaatagaca gccagaggtc cgggtgaccg ggttgttttt ctaccgccgc gagcagatat
     9841 ttcagcgctt cgtcaggttt ttcattccgc agcaaggctt tcccaagttt ttcctgaatc
     9901 tcaggctcat cttgcgcata caggttcgcc agccgaagag actcaacagg tctttcagca
     9961 ttaccgagcc gctgccataa ctcagcgaat ttaacatgcc agcggtattc gtttggccat
    10021 agttcaagcg cctgcgccag gctttcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnac atgccagcgg tattcgtttg gccatagttc aagcgcctgc gccaggcttt
    10201 cggcagccgc tgttaagttg ccagtacgtt caagtaaatc ggcgcgtaat cttagtgaaa
    10261 gaggttgctg cggttggatg cgcagtgacc gctcgagaat ttcaagcgct ttgggcacat
    10321 cattttcggc agtgcagaca cattgttcaa acatcaccag cggatgttct gcgaatttct
    10381 tgacaacctg aagggcagtt ctcgattgcc cgatacgccg taaagcggat gctaacgcgg
    10441 ctacatcttc tggcgttggt gtcagcagag ccagctcgcg aatattctcc ctggtcggct
    10501 ctttggccag ttgataacgc aggacccacc ggcgcgtttc ttccttcacc gcgtcattgg
    10561 ttattctctc catttcggcg ttgtcagggg tcggctcctt ctgttcactc aaagcacagt
    10621 gccgttcaac accaagtgat tccgccctat tagccatttc ttgcgccagc gcctggtgtt
    10681 tcaagtgcat caggcggttg acctggtggc tcgggacgag ccctgtcgct cgcgcgccaa
    10741 attgtaatgc ctccggccat cgttctaaag ccgctgccga tttagccagg tttcgaaaaa
    10801 tcccgatttt tacaataaac gggaaagcct gaacttctga aaacacattg atagccattt
    10861 caagggaggt ttcggccgcc gcatagtttc cctcatttgc caacagccga atgaatgaaa
    10921 tcttttctgg ttctccaacg ctgactgaat cgaaaatgtc accttccacc ggctcatctc
    10981 gaaccagtgt accgtcatcc agttctcgct cactcttcag caagttaaga taaccaacca
    11041 gtggatgttt tgggtccaag cggatctcat ccaggaaatg ttgataatcc tcctgcgaac
    11101 aacaggcgag cgccgtttcg atcccataga ggcgagcctc gaggttttct ggttctgcgt
    11161 tgatccaatc ccgaacatcg ttcatcaact cctcagggct tcccgctgtc atggcgatgc
    11221 gagacttcaa gacaatttcg ggtcttgagg aatccaacag ttccttccag tccatcggca
    11281 ggaagacctc atcaatcatg atgtccaggg gccttaagtg ttcatcgagg tctatatcca
    11341 accacgtccc ctcgcctgaa tgggtgaagg cggtctctct cttgaaaagt tccgtcattc
    11401 cagttgttgg ctgtaccgat tccaatacgg agagggcttt ttccagttcg cccgacatac
    11461 gatataagcc ggcaagagaa agttgcgcct gcgagctccc ctgggcagcg gccttttcaa
    11521 ggcaagagat ggtcttctca gggttcccca ggttggaaaa aatcatcgcg gctttgatca
    11581 acgcgccggc atcgtccgtt ttatcaatca acgcgagcaa gcattgctcg atttccagag
    11641 attcttcatc cgtgttgatg ccaattcgat tttgcgcctg gagtaacctc agcccaaggt
    11701 ggtctgggta ttgtgtaact gcgcttttta tgtgcttttc aagatttcca gttcgcccca
    11761 gcgctgttat aaactcaatc aaccagacgt ggttctcagg atggtcaggc gcaacttcaa
    11821 gcgccgattc tgcctgcttt accgcttcgt tcatatcacc tgataggttt aagaactctg
    11881 cttttagacg aagcaatcca tgccaggaag gctgaaggct gatcgcctgc tctaacgcag
    11941 ccatcgcgat ttcatgttca ccattctgcg cagcggattg tgctaatccg gcaaaaagcc
    12001 gggcggaagg cagttcccgt ccaccctgac tttcatccat aatcgcatca aataattttc
    12061 tcgctgcagt atattctcct gactggagca gccactctgc tttcagcatt ttggcgtgta
    12121 agttgtcagg ttcccggata agcaaatcat ccaggagctc tttggtaaat atcaactcac
    12181 ttttagcgag cctggcaggg ctcaccaggt gaacatagtc cggttgcaga acaccttcca
    12241 aatacgccat cgaccagaca gtatcttcct tcaggtgatc ggcaattgac ttcaaatacc
    12301 caagcccttt ttcctgttcg cccttccaga caagccttga cccgaattca ccgataaagc
    12361 gatggtcacc cgggtaccgg ttgacgaacc cagcgatggt ttcatcgata ttttccatgc
    12421 caagctgctt catggcagcg accagctgaa gatcaacttc agcagaatgc cctttcacag
    12481 agagttccag taagtacgga aacgcttcag acaccaaccc ttcatccatc aatcgctgag
    12541 cgtaaacgcg ctgcaattcc gcattacccg gattggcagc cattccttct ttgagcgtcg
    12601 tcaaggagga tgaatggtca ccacccgtcc aggcgatttc tgccaactcg atccagggtt
    12661 tgacctcccc ttcggcaaga gcgatcgctc tgcgcaattc atcgatcgct gcagcatcct
    12721 gtcctttacg gtgatgagcc atgccagcca acgtcaatcc tcgagcgctg tccgggtttc
    12781 tcccgagtat ttctgctgaa gccgtcagcg cttcatccgc tttcccaacc attaaggcga
    12841 tttcaccaaa gcggttcagg tctttttctt cgggcaggtc tgcctggtca ataagttcac
    12901 gatatgtctg gtaagcggcg tcataagcgt ttcccctgat ttgcgcatct gccagtttac
    12961 gcataacgcc tatcgaacct gggtcaaggg cgttcagtgt ttcaagatac cccagcgcgg
    13021 cagcatggtc tccttttcct tccgccgcag ccgctaacgc cgcaataaca ggttcactcg
    13081 aggggttacg cgctattaat actttaccga ggcgctctgc ttcatcgtaa gcgccgcttt
    13141 ctacaagcaa agtcagcagt gtgacgggct cccaactttg aatgtgttcc gggccatcga
    13201 caagcagccg ttcgaattcg ctttcagaaa gcagttttag gttttctcgc aattctgccc
    13261 gggcgagatc aacgttaccc gctttcgtca ttttctttgc ttgctgcata atcttcgcgg
    13321 tcgcggattc accgcttagc tgtaaatcac cggctgcgct tgtgtacgcg agctctgcga
    13381 gtatttctgg atcagccagt tcttcggttg aaaaaagttc accaagcgga atgctctcag
    13441 cggctgcatc gaaatggata tcggccttct ttaccttgct cattttgagc aacgctgaaa
    13501 taacctcgag cgctttttca ttaaggtccg cggcaacagc cgtgttgcca gccagctgtg
    13561 cgatatccgc aaccgattgg cattgaaagg cgtaatgcat agacgaaacc gggtttttcc
    13621 agtaatctcc ggtggacttt tttgcgctgt ctggtggttg gtatttttcc aggattttgg
    13681 aggcaacctg cgcgtaaatg agaccgtcac tggttttctt caactgtttc aatgccgcta
    13741 cctgtatctg cgggcttagg ggatagaggt agcggcgcag gacctccact ttttcctgct
    13801 cggtccaggg caggcacaaa acgagatggg tgaagatttc tatactgctt tccgcctgct
    13861 gaaaaatcgt gaggtcgcga aaacatgagt ctttatcttc aatcaaattc gtcaccacca
    13921 cgaagacagt cccccataag aggagaaacc tatctccgtg gttaattttc atcctggtca
    13981 cgatctcttg aataatttct gcccaactcg cggccgtttt ccgtttttcc agcaaagaca
    14041 acgccagccc cccgacctgc gccagggtcg ccggtggttg atcaccctgg atgtactctt
    14101 cgtaactgaa catgaccttt tctaactttt ctcgggaggt cattctggcg ttcaaagtgg
    14161 aaaaatcgaa ctcagggtca taaaggagga ctgtcaaagt acctgggttg agcggcattc
    14221 cctgccgggt taaagaggtt ataaccggag aatcttcagt gagttgttga aattcttccc
    14281 agatgaaagg atcctggcgc aatgctttaa actgaacgac agcctcttgt ttttgcgtga
    14341 ggtggttcag cgtggttatg agatcgagat aacgcatttc gtctcctctc ctggctatca
    14401 gagttggatt acccgcagac tttatatttt cgttaccagc aaataggcgg ccgctatcag
    14461 caacaacccc agaattacca ggaaaatccc gattcccgcg gtggtcttta ccatctggtc
    14521 aagcgcttca atcagagaac gcgcattgcc caccaggccc gaaatttcat cggcaatgcc
    14581 cttttgcgcg agtttggtgg tttggtctgc aatggtgcga atttccttcc cattgagctt
    14641 gccaatcaaa gtaaaaacgc caatccccag taccaccacg ccgcaaaata acaacacgct
    14701 cgccatggtc aacaggaagt cacttacagt cattgaaaac ataaagccct ccaaaatagt
    14761 tgctctggag ggtttgatgc aagtattatg ccatcattat ttgcttacgt tgaggtcagg
    14821 tccttaggaa ggtccgccag gttgatggtc attccatcac agaaaagcat agccctttca
    14881 atcgcgttag atagctccct gatgttgccc ggccaggaat actgctgcat tacctccatg
    14941 gctttcggcg aaacctcaac cacattcata ccctgcctgg cgttattttc gcggataaag
    15001 aatcccacca gttcaggaat atcctctttc cgttctctga gcggaggcat atccaggtcc
    15061 accaccttca accggtaata caggtcttct cggaagtcgc cttttttaat catctctttc
    15121 aagtttcgat ttgacgcggc gattatctgc acatccacct taatcaaagc agtgccgcct
    15181 acccggcgaa aggtgtgagt ttcgattgcg gtcagcaatt ttgcctgaat atccaccggc
    15241 attgaagcga tttcgttgag gaataaaacg ccgccatctg cgatttccat caaacccggt
    15301 ttttttcggt cagcgctggt gaaagccccc gcttcgtggc caaaaagttc cgattcaagc
    15361 attgtgcttt gaatggctgc acaattgata gagataaaag gcttgttcgc tcgtggccca
    15421 atagaatgaa tgtacttggc gatgacatct tttcctgttc cggtttcccc ggtgatgagc
    15481 acggaaacct gtttttcaga cgccaccagt gcctgttgaa cggtattttt cacaacttca
    15541 ttcgtgccga tcacgaaatt ccctttttgc atctgcgctt cacgatagtg ttctaactca
    15601 cggcgcatgg ccactatctc acgagcgcgt tgcaacgact tttccaatag cgacatatcc
    15661 aaaggttttt gaagaaaatc agtggcgcca ttcttcatcg cgtcaaccgc catatcgata
    15721 ctgccaaagc cggtaatcac gatgattggt gggcgcgcgg gcatcatcgc tgtttcatac
    15781 agcaggttta aaccatattc tccatggagc tgcacatcca gcagcactac atcgccatcc
    15841 cctttcttca ccctttcacg agcctcatcc agtgtactcg cttcgaaaac ctcatacccc
    15901 ttctttttga gaaaggtgct aacgaagctc ctcgcattat cttcatcttc cacaaccaaa
    15961 acagataaac tcataggtca ttctccgatg tccgcaggca attgtacagt gaatactgtt
    16021 cctccgggaa aagattctac cttgatactg cctttatggg cgtttacgat ttgcttggtg
    16081 atcgccagcc ccagccctgt acccatcttc ctggtggtca caaagggctc aaaaatacgc
    16141 gttttgatat cctgtggaat ccccggccca ttgtctgaaa cactgatctc tacaagcggg
    16201 cgatggtcta cttccagaac ctttactgcc ttaatcgcca gcgtgtcgcc gttgtctgtc
    16261 atcgcttcaa gcgcgttgga aatcagatta gtgaaaacgt tttcaagcga gcgtggatct
    16321 ccataaattt tgggcaattg ttcttccacg tgcagtatgg gtttcacgtt caccctcgac
    16381 atgcgtggca gccaacgatc aatgatgcgc ttcagaaacg gttcaacttc aaatggctcg
    16441 tacttcagtt ccatcgggcg tgaaaacgct aaaaacgact ccatcaggtg gtcaagccgc
    16501 atgcaatctc cctgcatgcg gtttatcaca tcctggctgg ggtcattttc atccaatgtt
    16561 gcagcaagca gctgcagacc cgtggatatg ttattgatgg ggtttctcac gtcatgggca
    16621 aaagccgctg tgtaatcacc caacacagcg cgatgttcga gctgctgcgt aaccgccttg
    16681 ctttcctcgt gttcgctgat atccatcatc aatatttcca cagccgatac gctctcaccc
    16741 aggtgtactg gaattacttt catcagaact ggaaatgcgt gcccattcct gcggttaagc
    16801 acggccttgc ccacgttgag cgtttccttt cctttttggg catccacgag cgcgggtaac
    16861 aggcggtcac aaccgatcag aatgttttca taactttgcc cttttacctc ccaggctgcg
    16921 taccccaaca taaactcagc cgccgggtta atttgctcaa ttttcagcag attatcgagt
    16981 atgatcacac cttcattcat cgactcaaaa acctggttct tcaagttaac cgagtacatg
    17041 gaacgctcaa ttctttcatt gagattattc accagcactt gctgttgaaa aagagcaagc
    17101 agcattgaag cgagcatttc catcccgtcc aggctcaagc ttacgggaat accacccttg
    17161 cccgccgcga cgagcagacc aaaagccccc ccaccctcca gcatcggcgc ggaggcaaca
    17221 tactcaatat tcttcaccct ggcttcgcgg tgcagttcag tcataattcg agaaccggtc
    17281 acccagatgg ttggcttttc caacttcatc aagtcggtgg atggaagctt acgaggaaaa
    17341 tcttccacat tccccgaaac cgccactctt ttcaattgcg ggagagaaga gtccgcttga
    17401 taaaggcata ccgtctcaca acccaatatt tcacagataa tatccacgcc ctggtagatt
    17461 ttttcggcga tatctccggt gtctgctaag cgggaaaagc gggtgagcaa ccgatacgta
    17521 tcttcgagat ggagtttcct ctcttgttcg tccagtgttt ctggcagaac ccgaatgacg
    17581 gtccatttcc cacccttgtc gatgaagtca aaacgaacgc gcgccttcaa aggagtgagg
    17641 tctctccgtt tgatttgcgt ttccctgatc tctccagaaa aagacgtgga caaatccaat
    17701 ccggggaaca gaaaaccaac atttttacga aaaacatcgc gatgagaata agaaaccacc
    17761 ctcatcaacc ggctgttaat gaacaggatg cttctcgttt cgttctctac caaagcggca
    17821 ggggtttcat caaaatttaa tagtgcagtg atctcctcga gaccgggttc atttttccct
    17881 tttagcacgg acgagaacct gcctgaaatc gccctcattt tcttttaatc gtgacccttc
    17941 gtaaatgcgc cggtaaaatg ccctcaatcg ctcgatactt ggcaaccgtt cgcctcgcca
    18001 cttgaaaacc tctttcagtc aacatttcaa ccaattcaga atcactgtac gttttgggtt
    18061 ctttctcgat aatttccttg agaacactgc gcacgttcag gctgcggtca aagaaagaag
    18121 ccagcggaac gatcctttta ttgggtaatt gcaccgactt attcgcgaca gcgcgtgaga
    18181 tagtagattc gtgcacttcg agttccttcg aaagctgcgc acgcgtcacc ggcttgagat
    18241 acttatc
//
LOCUS       DAOSPM010000036        18130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_3101_length_18130_cov_12.564758, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000036 DAOSPM010000000
VERSION     DAOSPM010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18130
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3101_length_18130_cov_12.564758"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1337)
                     /locus_tag="PLW25_04700"
     CDS             complement(222..1337)
                     /locus_tag="PLW25_04700"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOS53822.1"
                     /translation="MEIARTPIRKVAISTGGGDAPGLNAVIRAAVLAGLERGWEMYGI
                     RDGYNGLLMPEKYLEGGVVNLTRDRVRGITHLGGTIIGTTNRGNPFSYPVKNLDGTYT
                     EVDRSDELVRAFNMHEFDALISVGGDGSMSIANSLSQKGLRVVGVPKTIDNDLDETVV
                     TFGFDTAVSFAVECLDRLHSTAASHRRILVVEVMGRYAGWIALESGIAGSADVVLIPE
                     IPYDINLVVKKIIDREKAGRNFSMVVVAEGAIPKGGTVTVQSKEIGRAERLGGVGEKV
                     TEALQELTGKEARLVVLGHLLRGGSPTANDRLIALRFGAAAVRALDEGQEGVMVALDP
                     PTVKYVPLSDATRRMKSVPLDCDTMLTARDLGMCFGD"
     assembly_gap    1722..1821
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1822..2176
                     /locus_tag="PLW25_04705"
     CDS             <1822..2176
                     /locus_tag="PLW25_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS53823.1"
                     /translation="LKQSALSLSGGQQQRLCIARALAVQPEVILMDEPASALDPIATL
                     KIEELMQSLKESYTIVIVTHNMQQAARASDYTAMMMLKEGTRSGTVIEFNETNHIFTR
                     PADKRTEDYVTGRFG"
     gene            2207..2869
                     /gene="phoU"
                     /locus_tag="PLW25_04710"
     CDS             2207..2869
                     /gene="phoU"
                     /locus_tag="PLW25_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOS53824.1"
                     /translation="MRATFETELKYLKDELLLLGNLVEQQIQGAVESFKQRDPQLSRT
                     IVEKDDEVNEKRYMIEEQVLITIATQQPMARDLRLLASILEIAGELERIGDYAKGIAT
                     INIRMGEEALLTPLVKIPMMAEKCSSMLHRAIQAFIQEDIEEANQIPREDDEVDRLYE
                     EVYSSMMEKIVHDENAIQRANWILWVAHNLERVADRVTNICERTIFTVTGDMKEIRSS
                     DQ"
     gene            complement(3015..4559)
                     /locus_tag="PLW25_04715"
     CDS             complement(3015..4559)
                     /locus_tag="PLW25_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53825.1"
                     /translation="MKTLYTTLALLIVAVFVLSACAPAAPTAAPVVPVETQAPEAAAA
                     TEAVVATEAVTPEQEEVSLWDQPYISQVPPILMIEPYYTIFGQSQVAVPYYYEEAVKL
                     AGHSCGATAGAWIITKKALEALYPEGETPVRGQIAVEAPGAENEFPVGVLGEIITFIT
                     GAAPKTGFIGQEFGEANNLFKRQNKMVYTDEPSGTPPPKMEWIFTRLDTGKKVGVNFN
                     LAIITPIATEERQAMGAKLAKGGATAEEAADYAVYWNDRAKFVFENADMDGFFNVTVY
                     EEGATETPPSIVSDVKTVAAEDFSWDQAYITEVPPIMMMEPYFQIFGQTQEPVPYYYE
                     EAVKLAGHSCGATTGAWTITRKALEALYPDGQIPVRGQIAVQAPGAEDEFPVGVFGDI
                     ITYVTGAAGKTGFIGQEFGKANDLFVRQNKMVYMDKPSGTLPPKMEWIFTRLDTGAKV
                     GVRFNLVVITPLPTPNRIAMGKKLALGEATPEQAADYYQYWNDRATYVLKNAEMDGFF
                     TVTVYE"
     gene            complement(4743..5528)
                     /locus_tag="PLW25_04720"
     CDS             complement(4743..5528)
                     /locus_tag="PLW25_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53826.1"
                     /translation="MKTKFKLGGILAIIAAIIGIIGHYALFFQFYQAGMHAEAAEPGC
                     EILLEWVHPLLANMGLLAAALFIVSAYGFFTQRRWAFSLSVFGLILTLLGSFFVNIPF
                     MAAGLPPVYFALFFPYTIIYFLMMVLVQRLSWSRTLLALLTGICYILCWMNGIASTSR
                     IITIGADLFTLVQKLHFVAMVGMAVVTAGILLKPREWQRVLGLSAGVTELIVGIPLGI
                     VTTQELGRFSLFSLAPILSLALVIIFVWPGVWKKWTGGEEKAE"
     gene            complement(5594..5986)
                     /locus_tag="PLW25_04725"
     CDS             complement(5594..5986)
                     /locus_tag="PLW25_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53827.1"
                     /translation="MKLKNLWLLILLVLLLSGCASKNPCPEGSVTYLESADEFPPDTS
                     SSLPPTKTDIEIRGKTITVDRVIEGPLCNDTWEGTVYVACDLTVQKWDIKPFFLSNGC
                     NLEIKPGTVVYVAAHNNAAYYKGCTTCH"
     gene            6120..6791
                     /locus_tag="PLW25_04730"
     CDS             6120..6791
                     /locus_tag="PLW25_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOS53828.1"
                     /translation="MKNLTGYLNDPIFRVLTEKEREQVVASAVRRPVKKGEYLVLQGD
                     IWEYLFFIEQGSVVAEKGSFDGRTFVAATFKPGDLFWGLGFFLDNAVMPANLRVFEDG
                     VIIQWAKRDLKPLLESNGRFSWELCKLVMSKIQYVSEVLETMTFQPIAVRLARLLMSF
                     SDKEERVPIERSLTLDDMAARIGSTREMVCRLLYKFSDEGLIRITRTEFTITDPENLE
                     KRAQK"
     gene            complement(6794..7666)
                     /locus_tag="PLW25_04735"
     CDS             complement(6794..7666)
                     /locus_tag="PLW25_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOS53829.1"
                     /translation="MPKRKTKTRTSVKALKGVLTLVFRTLKGAFTTFILAKGAEAAAS
                     ISYYTLFSIFPLLLSILAIGSFIIDQATVERELLNFLPNLLPVSQDFIMTNVQQVFKL
                     RGAISSVAVVGLIWSATAVFTTLIRNINAAWPAAAPHSFIRMRLWSLAIIAAMVLLLI
                     LSSFSLTLRHLLNNLGIPINYSLLSEFLSSFFYKKVLPVLVEVLVFFSLYYWVPQIKV
                     KKMGALTGAVVATFSWQLITAIFSAYLASGLARYEIVYGSLGKMIALLAWIYFSSWII
                     LFCAHLTSSIDRHS"
     gene            complement(7690..9513)
                     /locus_tag="PLW25_04740"
     CDS             complement(7690..9513)
                     /locus_tag="PLW25_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53830.1"
                     /translation="MPDNYFDEEEFETRFNGKTLSRILKLTVPHIKWVIGFLLTIAIV
                     AGLDSIGTYLVKMVIDNGILAGDRTAVIKVLIMYGVLIFVQSGSTFLFIYLAGILGER
                     IRYDLRKKMFNHLQALSLNYYSKTPVGWIMARVTSDSERVADLVTWGLVDSTWGLMSI
                     VTSMVFMVAINWKLALIVLALLPVLFIIATYFQRWILASYRAVRKTNSKIVGAFNENI
                     TGVKVIKALGREPENLDEFKAITEKMYHSSFRAAWLSALFLPTVQIISALAVAAVVWY
                     GGGQAKIGALTVGGIQAFVSYITFMMWPIQDLARVFAEMQQAIASAERMFSLIDSEPQ
                     IQDRPKAIDPGTIKGDIIFDHVTFRYEKDEKPVLNDFSLHVRRGETIALVGPTGGGKS
                     TIINLLCRFYEPESGRIYIGGRDYTTLSLQSIQSRIGVVLQTPHLFSGSITENLRYGR
                     LDASMEEIISATKVIGAYDFIMHFEKGFDTEVGEGGGLLSVGQKQLLSLARAVLANPE
                     VFVMDEATSSVDTLTEALIQKGMQALLDTTTSFIIAHRLSTIKKADRILVIEDGRIAE
                     MGTHTELLRIHGKYHQLYTQQFRSQLETAYNPFQQAATASD"
     gene            complement(9503..11317)
                     /locus_tag="PLW25_04745"
     CDS             complement(9503..11317)
                     /locus_tag="PLW25_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53831.1"
                     /translation="MPPSSHMLDLDLKSTISHHRLAGIWKMMKGFRLRYIGAVTSLGL
                     SALAKTFTFLLLRYFVDTYLIQQDNRIGLPYIALGFVGLAILEGGFTFLSGVLSSQTA
                     EGITRRLRNYLYDHIQHLSFTYHSKTKTGELIERSTSDVDALRRFFSDQATNIGRILM
                     LFIINFVTLLNLNVKLAWVSIIVIPFILAVSIFLFKKVSSAYESYQQQEAILSTTLQE
                     NLSGVRVVKAFSRQRYEMDKFEKDNWEKYTRGKKLLIMHSLFWPVSDILCGAQLIAGY
                     LIGAMMAINGQISVGTYLAYSGLVIYIIYPLRNLGRIIVQTSTGLVSYSRVMDIIKEK
                     REPLDQGDYTPQSNLRGDFAFLNVGFEYEPGDQTLADINFSVKPGQVIALLGSTGSGK
                     STLVHLLPRFYEYTSGKILLDGVELNRYPRKFLRQQIGIVEQEPFLFSRTIRENITYG
                     VGREVSDEEVEQAARAAAIHDVILSFQEGYQTIVGERGVTLSGGQKQRVTIARTLLKN
                     PRILILDDSTSSVDTETEAEIREALWNLMRNRTTFIIAHRIQSVADADLILVMDKGRI
                     IQHGRHADLINQTGVYREIFNIQARIEDELEKELASAG"
     gene            11469..12584
                     /gene="ugpC"
                     /locus_tag="PLW25_04750"
     CDS             11469..12584
                     /gene="ugpC"
                     /locus_tag="PLW25_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOS53832.1"
                     /translation="MASVTYENVVKKFGDFTAIENMNLEIHDEEFLVLVGPSGCGKTT
                     ALRCLAGLEEVTSGKIYIGDRLVNDVPPKDRDIAMVFQSYALYPHMSVFDNMAFGLKL
                     RKLPKQEIKQRVEKSAKILGIENLLKRKPRELSGGQRQRVALGRAIVREPKVFLLDEP
                     LSNLDAKLRVQTRAEISKIHNKLRTTFVYVTHDQTEAMTMASRIAVMNKGKLQQIDTP
                     QTLYDQPANMFVAGFIGTPAMNFFTMRLIKDGDSLLVDNGEFKIKIPEDRNKLLMHLV
                     DKEVVFGIRPDDIHNPDYSPLNIHGERVKAKVDVVELMGNEILVYLVSDKGNNFVGRV
                     DPRSKYVIGETAEVVFNMDNSHIFDPSQDPDNPVAVR"
     gene            12663..13409
                     /gene="gpmA"
                     /locus_tag="PLW25_04755"
     CDS             12663..13409
                     /gene="gpmA"
                     /locus_tag="PLW25_04755"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOS53833.1"
                     /translation="MYKIVLLRHGESTWNKENRFTGWTDVDLTEKGEEEARTAGVLMK
                     AAGLEFDLAFTSVLTRAVRTLWIALQTMDMVWIPILNSWRLNERHYGALQGLNKAETA
                     EKYGDEQVLIWRRSYDTQPPKLTKDDERYPGNDRRYANLTPEELPLSECLKDTVARFL
                     PYWHGEIVPVIKSGKRVLIVAHGNSLRALVKYLDHVSDEEIVGFNIPTGVPLVYELDD
                     ELKPIRSYYLGDPEEVKRKAEAVANQAKKK"
     gene            complement(13438..14103)
                     /locus_tag="PLW25_04760"
     CDS             complement(13438..14103)
                     /locus_tag="PLW25_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HOS53834.1"
                     /translation="MKVLIRFISILISVVLPFFLLMCSIRMLLNPFFLDYEYNLPNFP
                     ADDFGFTTPDRLKWGKISLLYLTNNESDDYLGTLKLDDGSPLFNQRELSHMLDVRVLV
                     KAALKAWYGVGLFLFLSLALFWRTNNIKRYWQAVSTGGWLTLGLIVLMIAGVFIDFYQ
                     LFAGFHAIFFEGDTWLFFTNDTLIRLFPEKLWSDAFIYLGVFTLISALVCACAGRRLA
                     QRK"
     gene            complement(14100..15656)
                     /locus_tag="PLW25_04765"
     CDS             complement(14100..15656)
                     /locus_tag="PLW25_04765"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOS53835.1"
                     /translation="MRRYVYVVVFISGLASLAVEMSASRLLGNYFGTSNLVWASIIGL
                     ILIYLTAGYFIGGAWADRSPRYLTFFGVLAWSSLAIALIPLASRPILRLASTAFDNLQ
                     IGVLAGSFTAVMILFIIPVSLLGMASPFAIRLSIQDKENAGKISGRIYAISTLGSFIG
                     TFLPVLVLIPLIGTYRTFLVISTVLMTFSLIGIYLDGRWKWLVRYAWMPLVLIALFLW
                     GTPGSDKSTQGLIFEGESAYNYIQVLDEDDFRLLRLNEGQGVHSIYHPTILNFNGPWE
                     QVMSAPFFNPAPVEPASIKSAAIIGLAAGTTSRELFAVFPHVKIDGVEIDPLIVDVGK
                     RYFHMENPNLNVIVQDGRWALERSAEKYQVVSIDAYRPPYIPWHMTTVEFFQSVRAHL
                     AEDGVMIINIGRGPTDRRLVDALAATIRQVFPSIYISDLSGSFNTLLFATVQETSLEN
                     YLANYVHLMENPNTPPLLLEVLAATYEGLQPLPEDEGLVFTDDHAPVEQITNSIVLNF
                     LFSGGTKNLE"
     gene            15725..17320
                     /locus_tag="PLW25_04770"
     CDS             15725..17320
                     /locus_tag="PLW25_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOS53836.1"
                     /translation="MKLISVAAMRDLEQRANAAGFTFMAMMQTAGERIALYVDQKHRR
                     GVQPYALGLIGGGNNGGDTLIALSLLQSRGWNTRAILARDRKPHDVLLQEYCRSGGAV
                     VDAAKMNELQHLEGIVLDGIYGTGFRSPLPEPIQNLLSDVSESLPGFAWIAVDCPSGM
                     DCESGEVSRGTVKAAETVCLEAVKRGMLTYSAFPYLGELITVDLGIARSLGRAGEPGD
                     MVITDELVHALLPKREAFSHKGSYGKALVIGGSANYPGAPVLAGRGAYAVGTGLVQVA
                     VPASVYPAGVTSCPELTWVLLEDAGGCISEIAAETVILYWMGCDSAVLGPGLGRHDST
                     RRFVFNLLLRENLAQGEVAGFHGVARRQKTKYRDSGRPPLVIDADGLTLLGEDSAWFE
                     KSNLTAVLTPHPGEMAHLTGLSVEEVQSDRMEVARRFALQWGQTVVLKGPLTVVAGTE
                     GHIAVLPVATSSLAKAGTGDVLAGMIAGFLAQGLSTWEAALVGAWMHAQAGLATRKIV
                     GCDESVLAGDVIRAIPEVYKLIE"
     gene            complement(17367..17693)
                     /locus_tag="PLW25_04775"
     CDS             complement(17367..17693)
                     /locus_tag="PLW25_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOS53837.1"
                     /translation="MEERDNFGAFLVGFLIGGITGAVVSLLYAPKSGDETRTVIKEKA
                     IELKDKTAVTAEEAYRKAEQAANEAAAKAQELLKTAQSKAAEVLKKAPVVLEEEKVVV
                     KAKKAA"
     gene            complement(17721..>18130)
                     /locus_tag="PLW25_04780"
     CDS             complement(17721..>18130)
                     /locus_tag="PLW25_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53838.1"
                     /translation="EGEMVESIPVKKNKTWLYILIAVVIIGLLVTGIVLLATAGNTTT
                     TMVRDIFIILMALESIVIGVALVILVIQLSSLINLLQNEIKPILQNTSDTVNTLKGTT
                     TFLSDNLATPVIKLNSSVAGLRKLLNLIGLFRK"
BASE COUNT         4712 a         4372 c         4700 g         4246 t
ORIGIN      
        1 acgcgagtat tgagcattca tgtaaagtgt gtatccaaat atccgtcagg gtacacgtct
       61 tcaccctccc gcatggatgc gcgcgtaaaa ttcatcaggc gctaatttaa cggagagcct
      121 gggaaactga aaccatctcc acgtacgtac ggcgatgaaa aaggggtaaa aagagcaacc
      181 gcgatggatt ttctgctgcc caccccgacc taaaaagaaa atcaatcccc aaaacacata
      241 ccaaggtcgc gtgcggtgag catggtgtcg cagtccagcg gtacactctt catgcgcctg
      301 gtcgcgtctg acaggggcac gtacttcacc gtgggcgggt cgagcgccac catcacacct
      361 tcctgcccct catccagcgc gcgcaccgct gccgcgccga aacgcagcgc aataagccgg
      421 tcattggcag tgggcgaacc gccgcgcagc aggtgaccca acaccaccag gcgggcttcc
      481 ttaccggtga gctcctgcag cgcttcagtg accttctctc ccacgccgcc caggcgctcc
      541 gcgcgcccaa tctcctttga ttgcacagtg accgttccgc ctttgggaat ggcgccttcg
      601 gcgactacca ccatcgagaa attccgcccg gctttctcgc ggtcgattat ttttttcacc
      661 acaaggttga tatcataggg aatctcaggg atgagcacca cgtcggctga gccagcaata
      721 ccggattcca gcgcgatcca gcctgcgtag cggcccatca cctccaccac cagtatacga
      781 cgatggctgg ctgctgtgct gtgcaggcgg tccaggcact ctacagcgaa actgaccgcg
      841 gtatcaaaac caaacgtgac caccgtttcg tccagatcgt tgtcaatcgt cttaggcaca
      901 cccactaccc gcagcccctt ttgcgacaat gaattggcga tagacatgga gccgtcaccg
      961 ccaacggaga tgagcgcatc aaactcgtgc atattaaaag cgcgtaccag ctcatccgaa
     1021 cggtcaacct cagtgtaagt gccgtccagg ttcttgaccg ggtagctgaa cgggttgccg
     1081 cggttggtgg tgccgatgat ggttccacca aggtgggtga taccgcgcac gcggtctcgc
     1141 gtgaggttga ccacgccgcc ctcaaggtat ttctcgggca tcaataagcc attataacca
     1201 tcgcggatgc cgtacatctc ccagccgcgc tccaaccccg ccagcacagc agcgcggatg
     1261 accgcgttca accctggggc gtctccgccg ccggtggata tggcaacttt acgtatgggt
     1321 gtacgggcga tttccatggc aaccttccat tttggagtta taagaatact tgaattataa
     1381 aagaaatctg gtcagtggaa agcagagatg aaacgaaaat ccttttttac cgcggcgaca
     1441 gggaggaccc acttctgtgg gtgtttcgta agaaagaaac ctccgggata ccgttgtgat
     1501 acaatcttat tttaagccgt gaagaaccgg tgtaaagggt cgtacagaga ggtgtgggcg
     1561 attaattgga gaaaaccatc aggatgaata ccgccaagat cgaaacgcat aacctcgagg
     1621 tcttttacgg aagttttaag gccatcacag acgtgaacgt caagataccc gaaaaacgca
     1681 tcaccgcttt tatcggacct tccggctgcg gaaaatccac annnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nccttaaaca atccgcgctt tcgctatctg gcgggcagca
     1861 gcaacgcctg tgcatcgcgc gcgcgctggc tgtgcagccc gaagtgatct tgatggatga
     1921 accggcgtcc gcgctcgacc caatcgcgac tttgaagatt gaagaattga tgcagtcact
     1981 taaggagagt tacaccattg ttatcgtcac tcacaatatg cagcaggcgg cgcgggcttc
     2041 agattacacg gcgatgatga tgttgaaaga aggcacgcgt tctggcacag tgattgaatt
     2101 taacgagacc aaccatatct tcacgcgccc tgcggataaa cgcacggaag attacgtgac
     2161 cggaagattc ggataaatgt tacaatgaaa ataaagcgag ggaaatatgc gcgcgacatt
     2221 cgaaacagaa ttgaaatatt taaaggatga acttctgctc ctgggcaatc tggtggaaca
     2281 acagatccaa ggagcggtcg agtcgtttaa gcaaagggac ccacagcttt cgcgaacaat
     2341 tgttgaaaag gatgacgagg ttaatgaaaa acggtatatg atcgaggaac aggtgctcat
     2401 cactatcgcc acccaacagc ccatggcgcg cgacctgagg ttgctggcgt cgatccttga
     2461 aattgctggg gaactcgagc gcattggtga ttacgccaaa ggcatcgcga ccatcaacat
     2521 ccgcatgggc gaggaggcac tgctcacccc actggtaaaa atcccgatga tggctgagaa
     2581 atgctccagt atgctgcacc gcgcaatcca ggcgtttatc caagaagata tcgaagaggc
     2641 gaatcaaatt cccagggaag acgatgaagt cgaccgctta tacgaagagg tatacagctc
     2701 gatgatggag aagatcgtcc atgatgaaaa cgccatccag cgcgccaact ggatcttatg
     2761 ggtggcgcac aacctggaga gggtcgctga ccgggtgacg aatatttgcg aacgcaccat
     2821 ttttaccgtt accggcgata tgaaagagat caggtcttca gaccaataaa agacctgagt
     2881 ggcccgcatg gcctgaaaaa tgaacgcccc aaagacgagg gctgagttta accttacatc
     2941 ttttttactt gaaacaggaa aaacaaaata gcggctgacc agccgctatt ttgctctcta
     3001 ttgcactaac agggttattc gtagactgtc acggtgaaga agccatccat ctccgcattt
     3061 ttcaatacat aagtggctct gtcgttccag tattggtagt aatcagccgc ctgttccggg
     3121 gtggcttcac caagcgccag cttcttaccc atggcgatgc gattgggcgt tggcagtggg
     3181 gtgatcacca ccagattgaa cctcacaccc acctttgcgc cggtatccag cctggtaaag
     3241 atccattcca tttttggcgg aagcgtgcca gagggtttat ccatatacac cattttgttc
     3301 tggcgaacaa agaggtcgtt ggctttaccg aattcctggc cgataaatcc tgttttgccg
     3361 gcagccccgg tgacgtaggt gattatgtca ccgaaaaccc caacggggaa ttcatcttca
     3421 gctccaggag cctgtacggc aatctgcccg cgcaccggga tctggccatc tggataaagc
     3481 gcttcaaggg ctttacgtgt aatcgtccac gcgcctgtgg tcgctccgca ggaatgcccg
     3541 gccaatttta ctgcttcttc gtaataataa ggcacaggtt cctgggtttg accgaatatc
     3601 tggaaatagg gctccatcat cataatggga ggaacctcgg tgatgtatgc ctggtcccag
     3661 gagaaatctt cagcagcgac tgtttttaca tctgagacaa ttgaaggcgg ggtttcagtg
     3721 gccccttctt catacacagt cacattgaag aaaccatcca tatccgcatt ttcgaaaacg
     3781 aatttggctc ggtcattcca gtacacagca taatccgcag cttcttctgc agtagcgcca
     3841 cctttggcca gcttcgcgcc cattgcctgg cgctcttctg tggcgatagg ggtgatgatc
     3901 gccaggttaa aattcacgcc tacttttttg ccggtgtcca gcctggtgaa gatccattcc
     3961 atttttggcg gaggggttcc cgaaggctca tccgtgtaaa ccatcttatt ctgccgtttg
     4021 aagaggttgt tggcctcacc aaattcctgg ccgataaacc ctgttttggg cgcggctcca
     4081 gtgataaagg tgataatctc tccaagcacg ccaaccggga attcattttc agcgcctggg
     4141 gcctccacgg caatctgtcc acgtacagga gtctctcctt ctggatagag tgcttcaagc
     4201 gctttcttcg taatgatcca cgcgccagcc gtggctccgc aagaatggcc agccagcttg
     4261 accgcttctt cgtagtagta aggaaccgcc acctgcgatt gcccaaaaat ggtgtaatac
     4321 ggttcaatca tcaagatagg cggaacctgt gaaatgtatg gctggtccca aaggctgacc
     4381 tcttcttgct caggcgtaac cgcttcggtt gcaacaactg cttctgtagc agccgctgct
     4441 tcaggcgcct gagtttcgac cggcaccacc ggagccgcgg ttggggcagc aggggcacaa
     4501 gccgaaagga caaaaacagc aacgatcagt aatgccaatg tggtgtacaa cgtcttcatt
     4561 tctcttctcc ttgtgtgtga gttgaacgaa caaaaagtta tttgtgtgca tcattctctt
     4621 tccgtacaaa tggtgaagga tcacgcggac ccttcaccat agataggact tcaatgcgct
     4681 tttagtcgtt ttgaggatga aaatggggtg gtagatgtac tccatgtttt ggacagggtc
     4741 ggctactccg ctttttcttc tcctccggtc cattttttcc aaacgcctgg ccaaacaaaa
     4801 atgatcacca atgccagtga aaggatgggg gcgagcgaga ataacgagaa acgacctaat
     4861 tcctgggtgg tgacgatacc caacgggatt cccacgatca attctgttac cccggcgctt
     4921 aacccgagaa ctctctgcca ctcacgcggt tttagtaaaa ttcctgctgt gaccacagcc
     4981 atgccaacca tcgcgacaaa gtgaagcttt tgaaccagtg taaacaggtc agcaccaatt
     5041 gtgatgatgc ggcttgtgct ggcaatcccg ttcatccaac acaagatgta gcaaatgccg
     5101 gtcagcagag ccagcaaagt gcggctccac gataaacgct gcaccagtac catcatcaga
     5161 aagtagatga tcgtatatgg gaaaaagagg gcaaaataga ctggcggaag ccctgcagcc
     5221 atgaatggga tattgacgaa aaagctgcca agcagcgtta ggatgagccc gaacactgat
     5281 agcgaaaaag cccatcggcg ctgtgtaaag aagccgtaag cgctgacgat gaacaacgct
     5341 gccgctaaca atcccatatt cgccagtaat gggtgaaccc actccaggag gatctcgcag
     5401 cccggttcag cggcttctgc gtgcatgcct gcctggtaga attggaaaaa gagtgcatag
     5461 tggccgatga tgccaatgat ggcagcaatg atggccagga ttcccccgag tttgaatttt
     5521 gttttcacgt tttctcctta aaaattatga tgaacagcct ggtgttctga gttgttaacc
     5581 tctatgaaaa aaatcaatgg caggtcgtac aacctttgta gtaagcagcg ttgttgtgcg
     5641 cggctacgta gacaacggtg cctggtttta tctctaggtt acagccatta gagagaaaga
     5701 aaggtttaat atcccatttt tgaactgtca ggtcgcaggc aacataaacg gttccttccc
     5761 aggtatcgtt gcacaaagga ccctcaataa cgcgatcaac ggtgatggtt ttccctcgga
     5821 tttcaatatc tgttttggta gggggtaagg aagatgaagt gtccgggggg aattcgtccg
     5881 cgctttccag gtaggtcacg ctaccttcag ggcacgggtt ttttgatgca caaccggata
     5941 aaagtaaaac caataggatg agcagccaaa ggttctttaa tttcatattt ttctccagtc
     6001 aggtttcttc aattatgaca aaaacagcgt tgcatgactt tgcttttagt taaattgcgg
     6061 gcatttctgt tgtgactggt atagaattag ttgggatatg aaaaccgggg aggttgctta
     6121 tgaaaaactt gacaggttat ttgaatgatc caatcttccg cgtgttgacc gaaaaagaac
     6181 gcgagcaggt tgtcgcttca gcggttagaa gaccggtgaa aaaaggcgag tacctggtgt
     6241 tgcagggaga tatttgggaa tatttgtttt tcattgagca gggttccgtg gttgctgaaa
     6301 aaggctcatt tgatggacgc acatttgtcg cggctacctt taaacctggc gacctttttt
     6361 gggggttagg cttcttcctg gataacgcgg ttatgccggc gaacctgcgt gttttcgaag
     6421 atggggtcat tattcagtgg gcgaagcgag acttaaaacc tctgcttgaa agtaacgggc
     6481 gtttttcttg ggaattatgc aaactggtga tgagcaagat ccagtatgtg agcgaggtat
     6541 tggaaacgat gacctttcaa cctattgccg tcaggctggc gcggctgttg atgagcttct
     6601 cagacaaaga agagcgggtg ccaatcgagc gcagcctgac cctggatgat atggctgcgc
     6661 gcattggcag cacccgagaa atggtctgcc gcctgttata caaattttct gatgaagggt
     6721 taattcgcat cacccggaca gaatttacca tcactgaccc cgagaacctg gaaaaacgcg
     6781 cccaaaagta acttcaacta tgccggtcga tggatgaggt caggtgggcg cagaacagga
     6841 tgatccaact gctaaaatag atccatgcca gcagggcgat cattttcccc agtgacccat
     6901 atacaatctc atatcttgcc aatccactgg cgagatacgc gctgaatatg gcagtgatga
     6961 gctgccatga aaaagtggct accaccgctc cggtcagcgc gcccattttc ttaactttaa
     7021 tctgtggcac ccagtaatac aggctgaaaa acaccagtac ctctaccagc acgggcaaga
     7081 ccttcttgta aaagaaagag gaaaggaatt cactcaatag cgaatagttg atagggattc
     7141 ccaggttatt gagcaaatga cgcagggtca gcgagaaaga agacaaaatc agcagtaaaa
     7201 ccatcgcagc gatgatagcc agggaccaca ggcgcatacg gataaaactg tgcggcgcag
     7261 ctgccggcca ggcggcgttg atattacgga tgagggtcgt gaacacagct gtggcggacc
     7321 agatcagccc aaccacagcg acggatgaga tcgctccacg caatttgaat acttgctgca
     7381 cgttggtcat aatgaagtct tgtgatacag gcagcaggtt gggcaaaaag ttcagcagtt
     7441 cacgttccac tgttgcctgg tcaataataa aggagcctat cgcgaggatg ctcaatagca
     7501 aaggaaagat ggaaaacaaa gtgtaataag agatgctggc ggcggcttcc gctcctttag
     7561 ccaggataaa cgtggtaaat gcccctttta aggttctaaa taccagggtt aacacgcctt
     7621 tcaacgcttt tacgcttgtt cttgttttcg ttttcctctt tggcataagt attcatccct
     7681 taaatcatgc tagtctgacg ctgtcgcggc ttgctggaag ggattgtacg ccgtttcgag
     7741 ctgcgagcgg aactgctgag tataaagttg atgatatttt ccatgaatcc tgagaagttc
     7801 cgtgtgggtt cccatttccg cgatgcgccc atcttcgatc accaggatcc tgtctgcttt
     7861 cttaatggta gatagacggt gagcgatgat aaagctggtc gtggtatcca gcagggcttg
     7921 catccctttc tgtatcagcg cttcagtaag cgtgtcgacc gagctggtcg cttcatccat
     7981 tacgaaaacc tcagggttgg ccaatacagc cctggccagg cttaacaatt gtttctgccc
     8041 cactgagagc agaccgccac cctcgccaac ttcagtgtca aacccttttt caaagtgcat
     8101 gatgaaatca taagcgccga tcacttttgt ggcggagata atctcctcca tgctggcatc
     8161 caggcgaccg taacgcaggt tttcagtgat actgcctgaa aaaagatgcg gagtttgcag
     8221 caccacgccg atgcgggatt gaattgactg aagcgaaagg gtagtgtaat cacggcctcc
     8281 aatgtagatg cgcccggatt cgggctcata gaaacggcaa agaaggttga tgatggtcga
     8341 ttttccaccg cctgtaggac ccaccagggc tatcgtctct ccccgccgta cgtgcaggct
     8401 gaaatcgttc aataccggtt tttcgtcttt ctcataacgg aaggtcacat ggtcgaagat
     8461 gatatcgcct ttgatggtgc ctgggtcgat ggccttaggc cggtcttgaa tttgcggttc
     8521 tgaatcgatc aatgaaaaca tccgctctgc cgaagcgata gcctgctgca tttcagcaaa
     8581 cacacgcgcc aggtcctgga ttggccacat cataaacgtg atgtatgaaa cgaaagcctg
     8641 gattccgcca accgtcagcg cgccaatctt cgcctgtccg ccgccatacc aaactacggc
     8701 tgccaccgcc agcgcggaaa taatttgaac agtgggcaag aaaagcgcgc tgagccacgc
     8761 tgcccggaat gaagaatggt acattttttc tgtgattgct ttaaattcat cgagattttc
     8821 tggttcgcgc cccagggctt tgataacctt gacgccggtg atattttcat tgaaggcgcc
     8881 tacgatcttg gagttggttt ttctcaccgc gcggtatgaa gcgaggatcc agcgttggaa
     8941 ataggtggcg atgataaaaa gcactggtaa cagcgccaat acgatcagag ccagcttcca
     9001 gttgatagca accatgaaga ccatcgatgt gacgatgctc atcagccccc aggtagaatc
     9061 gaccagtccc caggtgacca ggtccgccac gcgctcagaa tctgacgtca cacgagccat
     9121 tatccagccc accggtgttt tactgtagta attcagcgac agagcctgca ggtgattgaa
     9181 catcttcttg cgcaggtcat aacggatgcg ttcgccgagg atccctgcca ggtaaatgaa
     9241 aaggaacgtg ctgccagatt gaacgaaaat taataccccg tacatgataa gcacttttat
     9301 cacagctgta cggtcgcctg ccagaatgcc attatcgatc accattttga ccaggtaggt
     9361 cccgatagaa tccagaccgg ccacgatggc gatggtcagt aaaaagccaa tcacccactt
     9421 gatatgggga acagtgagtt tgaggatacg gcttaacgtt ttgccgttga acctggtctc
     9481 gaactcctct tcgtcgaaat aattatccgg cactggccag ctccttctcc agttcatcct
     9541 caatgcgggc ctgaatgttg aatatttcgc ggtacacccc tgtttgattg atcaggtcag
     9601 cgtgcctgcc gtgttgaatg atccgccctt tgtccatcac cagaatgagg tcagcgtcgg
     9661 ctacgctttg aatgcgatgg gcgatgatga aagtggttcg gtttcgcatc aggttccaca
     9721 gggcttctcg tatctccgct tcggtttcag tgtctaccga agaggtggaa tcatcaagaa
     9781 ttaaaatacg cgggttttta agcagcgtgc gcgcaatggt aacacgctgt ttctgccccc
     9841 ctgaaagagt cacgccgcgc tccccgacaa tggtctggta accttcctgg aaagatagaa
     9901 taacatcatg gattgcggct gcgcgcgccg cctgttcaac ttcttcgtcg ctgacttcac
     9961 gccctacacc ataggtgata ttctcacgga tggtgcgaga aaacaggaac ggttcctgct
    10021 ccacaatgcc aatctgttgg cgtaagaatt tacgtggata gcggttcaat tcgacgccat
    10081 ccagcaagat tttccctgag gtgtattcat agaacctggg cagcaaatgt accagcgtgg
    10141 atttacctga gccagttgaa ccaagcaacg cgatcacctg cccgggttta acactgaaat
    10201 tgatatctgc gagcgtctgg tctccaggtt cgtactcaaa cccaacattg agaaacgcaa
    10261 aatctccccg caggttggat tgtggggtgt aatctccctg gtcgagaggc tctctctttt
    10321 ccttgataat gtccatgacc cggctataag acaccaaccc cgtggaggtt tgcacaataa
    10381 tcctgccaag attgcgcagc gggtagataa tgtagataac cagaccgctg taggccagat
    10441 aggttcccac agaaatctga ccgttgatgg ccatcatcgc gccgatgaga tagcccgcga
    10501 tcagctgcgc gccgcacaga atgtcagata ccggccagaa gagcgaatgc atgatcagta
    10561 attttttccc gcgggtgtat ttctcccaat tgtctttttc aaacttatcc atttcataac
    10621 gctggcgcga aaaggctttc accacccgaa cacctgagag attctcctgg agtgtggtgg
    10681 aaaggatggc ttcctgctgt tggtaagatt cataagcgct ggacaccttt ttgaaaagaa
    10741 agatggacac cgccagaata aatggaatga caatgatcga gacccaggcg agtttcacat
    10801 tgagattcaa cagggtgaca aagttgatga tgaagagcat caaaatacga ccaatattag
    10861 ttgcctggtc cgaaaagaaa cggcgtaagg cgtctacatc tgaagtggag cgctcaatca
    10921 attcaccggt tttggttttg gaatgatagg taaaagagag gtgctggatg tgatcataga
    10981 ggtagtttct aaggcgccgg gtaatacctt ccgcggtctg agaagacagg accccgctga
    11041 gaaacgtaaa tccgccttcg agtatcgcca gcccaacaaa ccccagggcg atatacggca
    11101 agccaatgcg gttgtcttgc tggatcaaat aggtatcaac gaaatagcgc agcagcagga
    11161 atgtaaaggt cttcgccagg gcggatagcc ccaggctggt aactgcgcca atatagcgca
    11221 gtcggaaccc tttcatcatt ttccagatac ccgccaggcg atgatgagaa atggtggatt
    11281 ttaagtccag gtcaagcatg tgcgaagatg gtggcaagat gtccctcaga gcgatggtga
    11341 acttgaatta taaagcacat tcaactgacc taatcaataa ggatatatga aagcgagaat
    11401 aagaaaagag tcagctttca ttttgttata taatacagtc actatactcg ttcaagaagg
    11461 aggaaagcat ggcaagcgtt acgtatgaaa atgttgtgaa aaagttcggc gattttaccg
    11521 ccatcgaaaa catgaacctg gaaattcatg atgaagagtt tctggttctg gtcggtcctt
    11581 ccggctgtgg aaaaaccacc gcgctgcgtt gcctggctgg gctggaagag gtgacttctg
    11641 ggaagatcta tatcggtgac cggctggtta atgatgtgcc gccaaaagac cgagacatcg
    11701 ccatggtatt tcagtcctac gcgctttacc ctcacatgtc tgtgttcgac aatatggcgt
    11761 ttgggcttaa attacgcaaa ctgcccaaac aggaaatcaa gcaacgcgtg gaaaaatccg
    11821 ccaaaatact cggaattgaa aacctattaa aacgcaaacc gcgtgaactt tctggcgggc
    11881 aacgacagcg tgtggcgctc ggcagggcaa ttgtgcgcga accgaaggtt ttcctcctgg
    11941 atgaaccgct ttctaacttg gacgccaaac tgagggtaca aacccgcgcg gaaatcagca
    12001 agattcacaa caaactgcgc accacttttg tgtacgtaac ccatgatcag accgaagcca
    12061 tgacgatggc gagccgcatc gcggtgatga acaagggcaa gctgcagcaa atcgacacac
    12121 cacaaacgct ttatgatcaa cccgcgaata tgtttgttgc cgggttcatt ggcaccccag
    12181 cgatgaactt ctttacgatg cgcctgatta aggatggtga ttctctactc gtggataacg
    12241 gagaattcaa gattaaaatc cctgaggatc ggaataagct cttgatgcac ctggttgata
    12301 aggaagtggt attcggtatc cggccagacg atattcataa ccccgattat tcacccttga
    12361 acattcatgg tgagcgtgtt aaagccaagg ttgacgtggt ggaattgatg ggaaacgaga
    12421 tcctggtcta cctggtatct gacaagggaa ataattttgt tggcagagtt gaccctcgct
    12481 ccaagtatgt cattggtgaa acagccgaag tggtcttcaa catggataac agccacatct
    12541 ttgatccttc acaggaccca gataatcccg tggctgtacg ataatgtgat tttgagtaaa
    12601 tagatgtagc gcaagtacca accttgcgct actttttaaa taaacaagaa agaggataag
    12661 cgatgtacaa gattgttttg cttcggcatg gagaaagtac ctggaataag gaaaatcgct
    12721 tcactggctg gacggatgtt gacctgactg aaaaaggcga ggaagaagcg cgcacggcgg
    12781 gcgttttgat gaaggcggca ggattagaat tcgacctggc tttcacttct gtgttgacac
    12841 gggcggtacg cacactgtgg atcgcgctgc agaccatgga tatggtgtgg attcccatcc
    12901 ttaactcatg gcgtctcaat gagcggcatt atggcgccct tcaaggtctg aataaagccg
    12961 agacggcgga gaagtatggc gatgaacagg tgctgatctg gcgcagaagt tatgacaccc
    13021 agccgccaaa actcacgaaa gacgatgagc gctaccctgg aaacgatcga cgctacgcga
    13081 acctgacgcc tgaggaactc ccgttgagcg aatgcctgaa agatactgtt gcccgttttc
    13141 ttccatactg gcatggtgag attgtacctg tcataaaatc gggaaaacgc gtgctcatcg
    13201 tagcgcatgg caacagcctg agagcgctgg tgaaatacct cgatcatgtt tcggatgaag
    13261 agatcgttgg gtttaacata cccaccggtg taccgttggt ttatgagctg gatgatgagc
    13321 tgaaaccaat caggagttat tacctgggcg accccgagga ggtcaagcgg aaagctgaag
    13381 ccgtggccaa ccaggcaaag aaaaaatgaa cacaaaccgg cagggaactg ccggttatta
    13441 ttttctttga gccagccttc ggcctgcgca agcgcacacc agcgcgctga tgagagtgaa
    13501 cacccccaaa tagatgaatg cgtcggacca aagcttttca gggaaaagac ggatcagcgt
    13561 gtcgttggtg aagaacaacc aggtgtcgcc ctcgaagaag atggcgtgga aaccggcgaa
    13621 aagttgatag aaatcgatga acaccccggc gatcattaac acgataagcc cgagagtcaa
    13681 ccagccaccg gtggataccg cttgccagta ccgcttaata ttgtttgttc gccaaaaaag
    13741 tgccaacgac aggaagagga atagccccac gccataccag gccttcaaag ctgctttcac
    13801 caaaacacgg acatctaaca tgtggctcaa ctcgcgctga ttgaacaagg gcgaaccatc
    13861 atccagtttt aacgtaccaa gataatcatc gctttcatta tttgtgaggt acaagaggga
    13921 aattttcccc cacttcaggc ggtcaggtgt ggtgaatcca aaatcgtcgg cgggaaaatt
    13981 tggcaggtta tactcataat caaggaaaaa agggttgagc agcatccgga tggagcacat
    14041 caataagaaa aatggcagga caacagaaat taatatagag ataaatctga tcagcacttt
    14101 cattcgaggt tttttgtccc tcctgaaaac aaaaagttaa gcacgataga attggtaatc
    14161 tgctcaacag gagcgtggtc atctgtgaag accaaaccct cgtcttctgg caagggctgc
    14221 aagccttcgt atgttgctgc caacacttcc aataaaagcg gtggagtgtt tggattctcc
    14281 attaagtgaa cataatttgc caggtagttt tccaggctgg tctcctgcac tgtggcaaaa
    14341 aggagggtgt tgaaagaacc tgagagatcg gaaatataga tggaggggaa tacctggcga
    14401 atggttgcag ccagagcgtc taccaaccgc ctgtccgttg gtccgcgacc gatattgatg
    14461 atcatcacgc catcctcagc gaggtgtgcg cgaacggatt gaaaaaactc cacagtggtc
    14521 atgtgccagg ggatgtaggg agggcggtag gcatcgatac tgaccacctg gtatttttcg
    14581 gcgctccttt ccagcgccca acgaccgtct tggacaatca cattcaggtt ggggttctcc
    14641 atatgaaagt agcgctttcc cacatcgaca atgagagggt ctatctcaac gccgtcaatt
    14701 ttcacgtgtg gaaaaaccgc gaacaattca cggctggtgg taccggcagc caacccaatg
    14761 atggcggcgc ttttaataga tgcgggctca accggcgcgg gattgaaaaa aggagcgctc
    14821 attacctgtt cccaggggcc gttaaaattg agaattgttg gatggtaaat agaatgaacg
    14881 ccctgtcctt cattcagacg cagcaaacgg aaatcgtctt catcgagcac ctggatgtaa
    14941 ttgtaagcag attctccttc gaaaatgagg ccctgggttg atttgtctga cccaggcgta
    15001 ccccaaagga ataacgcgat gagcaccagg ggcatccagg cgtaacgcac cagccatttc
    15061 cacctgccat ccaggtagat accgatgaga gaaaaagtca ttaagaccgt tgagataacc
    15121 aggaaagttc tgtaagtgcc gataagaggg atcagaacca acacgggtag aaatgttccg
    15181 ataaacgagc ctaatgttga gatcgcgtaa atgcggccgg aaatcttgcc ggcgttctct
    15241 ttgtcttgga tggaaagacg gatggcaaag ggcgatgcca tacccagcag gctgacgggg
    15301 ataatgaata aaatcatcac cgcggtaaaa gatccagcca gcacgccaat ttgcaggttg
    15361 tcaaatgcag tggaagccag gcggagaatt gggcgagagg caagggggat aagcgcgatt
    15421 gctaatgaag accaggcgag gacgccaaaa aaggtcaggt aacgaggaga gcgatccgcc
    15481 caggcgccgc caatgaaata acccgctgtg aggtaaatga ggatcagccc gatgatgctt
    15541 gcccagacaa ggttgctggt gccgaaataa ttcccgagca gtcttgacgc ggacatttcc
    15601 acagccaacg aagccaggcc agaaataaaa accaccacat aaacataacg ccgcatgcgc
    15661 atcattatac ttgagatgag gtaggcaacc ctcgttgtat aataattgtg ttcagggagc
    15721 tgagatgaaa ttgatcagtg tcgcggctat gcgtgatttg gaacagcgtg caaacgcggc
    15781 tggattcaca tttatggcga tgatgcaaac agcgggtgag agaatcgcac tttatgttga
    15841 ccaaaaacat cgccgaggcg ttcaacctta cgcattgggg ttaatagggg gaggaaataa
    15901 tggcggagat acactaattg cgctttcgct tttgcaatca agaggctgga atacgcgcgc
    15961 gattctcgcc cgagatcgaa agccacatga tgttctatta caggaatatt gtcggagcgg
    16021 tggggcagta gttgatgcgg cgaaaatgaa tgaattgcag caccttgaag gcatagtgct
    16081 ggatgggatt tatggcacag gcttcagaag cccattacct gagccaatcc aaaatctctt
    16141 aagtgatgtt tctgaatcct tgccggggtt tgcctggatc gctgtggatt gcccatcagg
    16201 gatggattgt gagagcggcg aggtgagccg cggcacggta aaggcagccg aaaccgtgtg
    16261 ccttgaagcg gttaagcgag ggatgctcac ttattcagcc ttcccttatc ttggagagct
    16321 tatcacggtt gacctaggta ttgctcgttc tcttggcagg gcaggtgaac ctggagatat
    16381 ggtcattacc gatgagcttg ttcacgccct cctgccaaaa agagaagcat tttctcataa
    16441 ggggagttac ggtaaggcgc ttgtgatcgg cggatctgcg aattatcctg gcgcaccagt
    16501 acttgcaggg cgcggagcgt atgctgtggg aaccggtctg gtgcaggtcg ccgtaccagc
    16561 ttcggtttat ccagcgggag tgacctcttg cccggaactg acctgggtgc ttcttgagga
    16621 cgctggaggt tgcatttcgg aaatcgcggc tgaaacagtc atcctttatt ggatgggctg
    16681 cgatagtgcg gtccttggtc cagggttagg caggcatgat tctaccaggc gttttgtgtt
    16741 taacctgctc ctgcgtgaaa atctggctca aggtgaggtt gccgggtttc atggagttgc
    16801 acgccgacaa aaaacgaaat acagagattc tgggcgacct cctttggtaa tagacgctga
    16861 cgggcttacc ctgctgggag aagactccgc gtggtttgaa aaaagcaatc tcacagcggt
    16921 gctcacccca caccccggag aaatggcgca cctcacaggg ctttcggttg aggaagtcca
    16981 aagcgaccga atggaagtag cccggcgatt tgccctgcaa tgggggcaga cagtcgtgct
    17041 taaaggccct ttgactgtgg ttgccggaac agaaggtcac attgcggtat tgcctgtggc
    17101 gacttccagc cttgccaagg cgggcacagg agatgtgttg gcgggaatga tcgctggttt
    17161 tctcgctcag ggtctttcta cgtgggaggc agctttggtt ggagcctgga tgcacgcgca
    17221 ggccggactg gctaccagga aaattgtggg ttgtgatgaa tcggtattgg cgggtgatgt
    17281 gataagagca atcccagaag tgtataaact tatagaatag aaaacaacct cctgtgaggg
    17341 aggttgtttt gataacatat tcagcttcag gctgcttttt tggcttttac tacgactttt
    17401 tcttcttcca aaaccaccgg ggctttcttg agcacttccg ctgcttttga ctgggcggtc
    17461 ttcaacagtt cctgcgcttt cgcggcggct tcattggcag cctgctcggc tttgcgataa
    17521 gcttcttctg cggtcacggc ggtcttatct tttaattcga tcgctttttc tttgatcacg
    17581 gtgcgcgtct catcaccaga ttttggggca taaagcaggc tcaccacggc acccgtgatt
    17641 ccaccaatca gaaaaccaac caggaacgcg ccaaaattat ctctttcttc catgtcatat
    17701 ctccttttta ttaagaatcg ttattttcta aatagaccga tgagattgag taattttctc
    17761 aatcctgcga cagaactatt gagcttaatt accggtgtgg ccaggttgtc acttaaaaaa
    17821 gtggtggttc ctttcaaagt attcactgta tcactggtgt tctgtaagat cggtttaatc
    17881 tcattttgga gcagattgat gagcgaggaa agttgaatca ccaggatgac caaagccaca
    17941 ccaatcacga tggattcaag cgccatgagg atgatgaaaa tatcccttac catggtagta
    18001 gtggtattac cagctgtagc cagcagaacg attccggtaa cgagaagccc tatgatgacc
    18061 accgcgatga gaatataaag ccaggtctta tttttcttga ccgggatgct ttcaaccatt
    18121 tccccttcat
//
LOCUS       DAOSPM010000037        17491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_3270_length_17491_cov_13.432381, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000037 DAOSPM010000000
VERSION     DAOSPM010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 17491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17491
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3270_length_17491_cov_13.432381"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            687..2651
                     /locus_tag="PLW25_04785"
     CDS             687..2651
                     /locus_tag="PLW25_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOS53839.1"
                     /translation="MKRWINFLSCGLIITLIWSSIIIQPASAQKEEDTVRVLIKYKNG
                     QMKTARSTLNASGAVIRYVFNDINTLALTISEKDLPGLYRNPDLLTIEKDPARYLIEP
                     FSTEPEALFNNTVDVNGQIIPWGLEAVQARDVWDINRDAVIDSGAPTGAGMKVCIIDT
                     GYYTDHEDLKDNISGISQVDDNWQRDGYGHGTHVAGTISAMNNPIGVVGVTPGTVDLH
                     IVKIFSDEGKWVVGASDLIAAVYSCSENGANIINMSLGGDTPSTIEEAVFQSLFDSGI
                     LLIASSGNLGTSAYSYPASYPSVLSVAAVNSNLQHAGFSQYNNAVDLAAPGVSVISTV
                     PTKIETVVVDGVTYPGYHIQYSAWGSASGELVSGGLCDSVGAWAGKIVLCQRGSVDLL
                     TKVTNVQSSGGLVALIYNNEPGVFGGTLGDGNSSLIPALTMSQSDGQYLLYNKLGKVT
                     AVTSTVMGGSSYGSWSGTSMAAPHVAGAAALVWSCKPDASNTEVRDALINTALDLGTA
                     GRDDYFGYGLVQAFTACSSLKPTAVELSSFSAQSQPAKVTLSWETSSELNTLGFNLYR
                     SSTLNGKRDRLNAILIRGDNPPGSLVGGFYEYTDKSFNPRMMRPSAELSGTILQQALN
                     GSRPYYYWLEEVDIYGHNKVYGPVEVRKTP"
     gene            complement(2813..>4321)
                     /locus_tag="PLW25_04790"
     CDS             complement(2813..>4321)
                     /locus_tag="PLW25_04790"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOS53840.1"
                     /translation="ERGGGMYNYSSDPSLAFVTFTNNSAREHGGGMFNSGCQPTLNHV
                     DFTGNKSEIYGGGMNNNGSNPILTDVTFSNNTANYGGGIYNWEYSSPSLLRVTFTDNS
                     AVYRGGGMYNVDYCDFHLDQVTFTGNTATDYGGGIFTSNSNPTLQAVRFIGNSTPNHG
                     GGIYNSYTHATLTDVTFEGNSAKNGGGMNNNNSDPKMTNITFMGNTASERGGGLYNYS
                     SDPVLKNATFYNNSALTYGGGIFNSAGSLLLDGATLTANTAGIYGGGMATDATNNRVN
                     NSIFWRNTAVTSGSQIYSFHSGMPIINDSVIEGGCPAGGVCTNIISSNPLLGPPDNYG
                     GPTWTIPLLVGSSAIDAGNDATCSPFDQRGVPRPQGPHCDIGAFEATGDPTSADLLSF
                     TAIGEEKAVKLVWETTNEVNNLGFNLLRATSVEGARTRINEQMIPSSQPGSLIGSEYK
                     FLDESARLLPVELGANKTFYYWLETVDIYGRSELQGPIIYKMGLQQKITKGK"
     assembly_gap    4322..4421
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4422..5514)
                     /locus_tag="PLW25_04795"
     CDS             complement(<4422..5514)
                     /locus_tag="PLW25_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53841.1"
                     /translation="MKLQIFGKSTFIFFFSLILCGISVNQAHADGMVIRVKPAGIESG
                     TCGETWENACSLPRAISLSSNGDQIWVAAGVYLPTSTADRASSFVMKKGVAIYGGFGG
                     TELELNQRDPSVNITVLSGDVGVPGNNTDNSYHVVSGSGADGSSILDGFTIEKGYADG
                     ENPYNRGGGLYAYKGSPVIRNVIFTNNYAASLGGGVFASSSNPSFINVEFRNNSSGDR
                     GGGVYNYVGSSTFTNVLFVENSAVQRGGGVFNYSSGSNLTGVDFVRNASQDLGGGMNN
                     YLSTVSLSDCSFTENSAVTSGGGMYNGGGTIELENVTFTGNTAKGGGGMLNSSHSNVT
                     LENVSFERNSASERGGGMYNYSSDPSLAFV"
     gene            6042..7310
                     /locus_tag="PLW25_04800"
     CDS             6042..7310
                     /locus_tag="PLW25_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS53842.1"
                     /translation="MPVSIQQKRKLRILLALPVAAILLWGIITPNGFRAAVEVNKWSL
                     AFIAHFYQPISSDQALPLPPASHPHAKLLLAREAIANGDDDLALAYITPLIGPDNPLV
                     TNAYAEITYRHEDFARAISAWEMAGNLTALHNATYELREKKLLDDALKAAISRYHLEP
                     ETSTSALATIYTDLKEYDQAVALLDQSMGEFPESKFFVTWLNTKSGVQFLQANDYAAN
                     NQLSEAELFYRASVNTNPNNWIAWKKFGWFYYYNFKDSESAANCFKGEMAAYPENGEG
                     QIDLATLYALEKQAQPAGYWYRQAISLRPDRGDFQLHYAKFLRDSKELDEAAEIFDRL
                     SQDFPDYIDAHYEAALTYAQLNQPAKAIQSIERVLQLMNPPVLRYYLLAGSLYEANGM
                     KEKAVEAFKNALLLDPQSPEALNGIKRLSE"
     gene            7323..8942
                     /locus_tag="PLW25_04805"
     CDS             7323..8942
                     /locus_tag="PLW25_04805"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOS53843.1"
                     /translation="MPRSRARFSSIILTDTQKARWSGYLYGAMLLVCAALWGFLMIYD
                     YVPLIGYVGLFLLGAGNALICRRLPRSTPLDVNILALLLLLPVSLALSHNRELSLVKV
                     HGVLLSIALFCILVNFLANLKRLKWAILALAATAVLLSVLGFFGADWSSGDWALPAKL
                     LASLPLPLTAIHQFTASGGIHVNTIGGTLALFAPLLISLLLDRGAYRRAFLRKHPRAK
                     LFTILYKFMIAAGTALVFFMLILTQSRGALLGTMCGVFILLVWKDRRFLFIIPILVVA
                     LLVGIWVFADGDPARFFLLLDTYSNPEGNTLQIRIEYWKNTLYLIQDLPITGAGIGTY
                     GKVFHDIYPFATPLAIVRPLFYAHNMYLAVAADMGLPALVIYLALFSSFFTMTLMAIR
                     KARSIVKTLLRGLLCGMSAHMVYGLMDNYMLGEKLGFSVWLFFAVCTAIYIHRETLVH
                     YYSEQPGEALNTTPRLFRQVARSRLRDLWWNLGAWLVISVIGISFILPHPLISLVITV
                     SGGTLLGIVMTRRFELQYQGEHARPEARTSP"
     gene            8973..9827
                     /locus_tag="PLW25_04810"
     CDS             8973..9827
                     /locus_tag="PLW25_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS53844.1"
                     /translation="MGSTPDSDWDLIITPRKKWFELQLQEVWNYRDLIALFVRRDFVS
                     RYKQTILGPLWFLIQPLLTSVVFTVVFGNIAGLSTDALPRLLFYMSGNVLWAYFSGCL
                     NGTATTFTSNAQLFGKVYFPRLVTPLSIILSNLITFAIQFVFFLGFFVYYLLRGSPVR
                     FTLWALCLPLLILLMAGIGLGFGIIVSALTTKYRDLQHLVVFGISLWMYITPVIYPVS
                     SIPEKYRWVAVINPITPIVETFRAGFLGAGNASWGMLGYSAAFAAVILFIGVVIFNQV
                     EKTFIDTV"
     gene            9885..11300
                     /locus_tag="PLW25_04815"
     CDS             9885..11300
                     /locus_tag="PLW25_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53845.1"
                     /translation="MKHNSNQRNKNASLFKMVGNFLFVYLCLVLFFVLSLTLVSLIPG
                     TKIKENIASSALTLKEQGDRPRILGNHKLNQLDNLTESVMLGIIYNIDEHQPLKSALK
                     AMFYAGSNSNEENYRVNSLYDQIQYAFRPNNQYPRYWHGYIVAIRPMLTFMPYSSVQK
                     TLQIAFLIFFGYLVALFSKKLSMLAALSFAISIVLVNISIIPVTVHASMVFFICFSAI
                     ALFLKTKDMSTEKYIIAFFIIGALTSFVDLLTAPFITFGFPAIVLLISRNTVETRNSF
                     KSVFSFVVYIGLSWMIGYALLWGTKWVLASIMLKENVIAEGINGILERIGRDIPFGNI
                     QHINSFDAIKKNLQTFYTFTISNYPISFVVIPLMIFYGFFAVIWHKKKYRLDTTIIIA
                     LIGLLPFFWFGVAANHSFIHAYFTSRILSVSVFAFSYVLIDIVDWNQIKKAYTALKSK
                     MSSNRKSLIGMFFRKHKEILL"
     gene            11297..12580
                     /locus_tag="PLW25_04820"
     CDS             11297..12580
                     /locus_tag="PLW25_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53846.1"
                     /translation="MRPVISVEHLTKRYELGVIGTGSLTRDLERWWARARGQPDPYSR
                     IGQKDPFRLAGQSILALDDVSFRVEQGEALGIIGRNGAGKSTLLKILSRVTAPSSGVV
                     KVKGRIGSLLEVGTGFHPELTGRENIYLNGAILGMKKAEVARKFDEIVDFSGIEKFID
                     TPVKRYSSGMYVRLAFAVAAHLDPEILVVDEVLAVGDAEFQKKCLGKMGNVASEEGRT
                     VLFVSHNMAAIQQLCTKSILMNSGRLELAGDSLQVVKKYLASNEEMAAEKKHAILARN
                     EQENFQLSKVFFADETGSTQNSFMSGESITIHLEIESETIFRGLIVTIGINDENGTRL
                     TVLHSDLSGVPLQVTGKHTVVTCNIPNLLLLPGKYYLDIKIYRGKDVLLWVPNAKTLF
                     VEPGNILKSGKLPPKEWGGFFVLDNQWQASEKTTR"
     gene            12550..13527
                     /locus_tag="PLW25_04825"
     CDS             12550..13527
                     /locus_tag="PLW25_04825"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS53847.1"
                     /translation="MASIGKDNPLVSVIVPLRNRFNLVDEAVDAVYKQNYRPIELILV
                     DDCSSQAYRPKIQAHAGFTVAVIRHQENQGPGASRETGRQRATGAYLAYLDSDDLWHP
                     TFLEKMVLQLKEKPETGMCYCKTRQFSGADLNSGSLRPRNEQAFDAILPTLLFGRPWS
                     TGACLWTRRAADQIGPWSEGWTNEDIEYEIRAGIFGIQISHLPEVLCYERETDDPQQL
                     SQATHKKALLQKFSSIKAMAITVLENKHALEESTRVLFVKKKMRPMVTDLFQAEEYEK
                     VIELCKLMNKIVKPLSKAWLLTALLLASVRYRKFPGAAKIFQLGCHFLT"
     gene            13524..14774
                     /locus_tag="PLW25_04830"
     CDS             13524..14774
                     /locus_tag="PLW25_04830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS53848.1"
                     /translation="MNKKTTILFITAYYPPHHGGGFGLRCRDVAEGLRRRGHSLTILT
                     NRCALRGCCEHQSEERTLRLLALRPAGDNPLRQILFDRRELLQVARVVRQVQPRVIYL
                     WHMQNLSNAILPFLAGQPTPLILDDGGSELIYLSRLQKRGLYFYKNAGEPRLKTWLKR
                     LVKTGARLVSGGRIVPDWQWPEGMRVLFNSPSALEHARAQGGFVRNASVIPSGLDIAR
                     FPFKAAPRIHTPVKIILPARIKEQKGCLDGILLVEELRRRGVPAQLLIIGEAQSAAYL
                     ALLRREVEARRLSGQVDIRPMAGQDEMSRLYREHDFCFFPSSFKTGFSRVPLEAMASG
                     CLLLTYGNEGSREVVQDGATGYLLPEGDVAAAAERVHASTLDHAGYLRILSTARRQVE
                     ERYSLEDYLQKVEALLSRAGETPS"
     gene            14771..15865
                     /locus_tag="PLW25_04835"
     CDS             14771..15865
                     /locus_tag="PLW25_04835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS53849.1"
                     /translation="MRNPPGSRATCVVHYPSLRVMAGNPYWAMLESGLKEAGITLLDE
                     PDAHKLRWLFKNRNRVDILHFHYFQFLYCNRGRSRARLVYVARFALFLLLARLLGYRT
                     VLTLHNLKPTHPLNPAWIDYLGHWLAANFTQRVIVHCHEARRLLAARYGRKNNVIEVA
                     HPHYLGYASGKPSKTEARKILRLPPRSPVFLFFGGVRRNKGLENLIQTFKEIEEREPT
                     LLIAGKPEPGAGYAEQLQSLAGKDPRIRFHFGYIPDEELALFFNASDIVVLPFSAILT
                     SSSTILAMSFARAVIAPRMGCLPELLEPGAGWLYEADSPGALLQTLGRSLKEDWRQAG
                     VTALERVASHTPAIFIRETLSCYGCTNSSA"
     gene            15872..16762
                     /locus_tag="PLW25_04840"
     CDS             15872..16762
                     /locus_tag="PLW25_04840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS53850.1"
                     /translation="MNLPTVSIVTPCYNAARFIRETIESVSGQRYPHLEHIVIDGGST
                     DGTLDILKAYPHLRWVSAPDRGQSHALNKGFAMARGELIGWLNADDTYAPGAVSTAVD
                     YLLQHPEAGMVCSDMWIVGENGKPLRLARGKPFDLVELLTENYVRQATVFIRRQLLNE
                     VGGVNESLHYCMDRALWLRVGVHHQVAYLPGVVSANFRFYWGSKTSLYTPGFVAEWLG
                     VLTDALDNPLYQPIPRAEVLKAIEKYQVRHQVALMRQAARQKDPRSTWRACAALLSRH
                     WRYLLRYPYLKTRALLAAKR"
     gene            16759..>17491
                     /locus_tag="PLW25_04845"
     CDS             16759..>17491
                     /locus_tag="PLW25_04845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS53851.1"
                     /translation="MNILYVQAGQAVGGSKESLYQILRSLPDEFARQVVLERGSDPGF
                     NERARRYASAVHLLPLPTWQQYRRRTLAERARAPLADAWRLLRCALAAFKIARVIRQE
                     RIDLVHTNNSMSPSGALAARLTATPHIWHVRESIGKHKQYPLLLGNLALRLMRRWSDT
                     VICNSDYTAEVFREMRIPVRVIPNGLDLADFAPDAGTPRELGSLADLPPGEKRVGMVG
                     NLTTKMKRHDVFLDIAALVKAAAPRT"
BASE COUNT         4045 a         4759 c         4390 g         4197 t
ORIGIN      
        1 gggggacaaa gaagctttgc tttcaaagat gaaaatgctt ggaaatttct tgggtgattt
       61 tttacaaagc gcctatagaa gtaaaaattt gggaacatat taattagtgg aatccgaaac
      121 tgggacaaag gaggatttac agcattcatg gcgataaacc atcaatacag ctaacctgtt
      181 gtaataaaaa cacatgagct cagattaatt cggcgtgaaa ctaatttacc attctttatg
      241 ccctgcgcct tttttctttg acaaatttca caacctcgcc aataattttt agtaaccgcc
      301 actgctgtat acaccaccct gcctatcgag ctaagcaaag tgagtgaaag catcatcccc
      361 cagccgcatc tcaaaggttc aaccgtttta ttagcgcaaa tcgggtctca acgcaaacct
      421 tacagtcaag aataaataat tccactgcat cctttcggtc tgttatgtcc tcgcaatgaa
      481 tgaggacatt caaagcgaag aaaaaaaaac cgaaccggtg ctgtgtaatg ccctttttcc
      541 tttgggtgca aacaaatccg cccggactcg tcattccctt cattacagtt tattttcaat
      601 tgcgcctttt ccatcgaaaa gccgggcggg ttctgatatg attaatgggg ttcaggtcgc
      661 ctgcaaacgt gattattgag ggagttatga aacgttggat aaatttcttg tcttgtgggc
      721 ttatcatcac tttgatatgg tcttcgatca tcattcagcc cgcatctgcc caaaaagaag
      781 aagatacggt tagggtttta atcaagtata agaatggtca aatgaaaacc gcgcgtagta
      841 cgctgaacgc gtcaggtgcg gtcatccgtt atgttttcaa tgacatcaat accctcgcgc
      901 tgaccatctc tgagaaagac ctcccaggtt tatatcggaa ccctgacctg ctgaccatcg
      961 agaaagaccc cgcgcgttac cttattgagc cgttctcaac cgaacctgaa gcgttgttca
     1021 ataatacagt ggatgtcaat ggtcaaatca ttccctgggg ccttgaagcc gttcaggcac
     1081 gcgatgtttg ggatattaac cgagacgcgg tgattgattc aggcgcacca actggggcag
     1141 gcatgaaagt ctgcatcatc gataccggtt attacactga tcatgaagac ctgaaagata
     1201 atataagcgg aatttcccag gtcgacgaca actggcagcg ggatgggtac gggcacggta
     1261 cgcacgtggc cggcaccatc tccgcgatga acaaccccat cggtgtagtt ggagtcaccc
     1321 ccggcacagt cgacttgcac atcgtgaaga ttttctccga tgaaggaaaa tgggttgtcg
     1381 gagcgtctga cctgatcgca gccgtgtatt cctgcagcga aaacggcgcg aacatcatca
     1441 acatgagcct gggcggagat accccctcta ccattgaaga agccgtgttt caatcgctct
     1501 tcgattccgg gatcctttta atcgcttctt ctggcaattt gggcacctcg gcatacagct
     1561 atcctgcctc ttatccaagc gtcctttcgg tagccgcggt caattccaac ctgcagcatg
     1621 ctggtttttc gcagtataac aatgcggttg atcttgccgc cccgggcgtg tccgtgatca
     1681 gcaccgttcc gacgaaaatc gagacagttg tagtggatgg agttacctac cctggttatc
     1741 atatccagta ttctgcctgg ggttctgcga gcggtgaact ggtcagtggc ggcctgtgtg
     1801 actctgtagg cgcgtgggca gggaagatcg ttctttgcca acgcggctcc gtcgatttgc
     1861 tcaccaaggt cacgaatgtg caatcatcgg gcggcctggt tgcgttgatc tataacaatg
     1921 aacctggcgt gtttggagga accctagggg acggcaattc atcactcatt cccgcactca
     1981 ccatgagcca gtcggacggt caatatctcc tctacaacaa gctgggcaag gtaacagcag
     2041 tgaccagcac tgtgatggga gggtccagct acggttcctg gagtggaact tcgatggccg
     2101 cgccacatgt ggcaggcgcc gcggcgcttg tttggtcctg caaaccagac gcgagtaaca
     2161 ctgaagtaag agacgcgctg atcaacaccg ctttagatct tggaacagca ggccgggatg
     2221 actattttgg ttatgggctg gttcaggcat ttaccgcctg tagctccttg aaaccaacgg
     2281 ctgtggagct ctcctccttc tcagctcaat ctcaaccagc aaaggtgacg ctgagctggg
     2341 agacctcaag cgaactcaac acactggggt ttaacctgta ccgctccagc acattaaacg
     2401 gaaaaagaga caggctgaat gcaatactca ttcgaggaga caacccaccc ggcagcctcg
     2461 tgggtggttt ttacgaatac actgataaat cattcaaccc aagaatgatg agacccagcg
     2521 cagaactcag cggcaccatt ctgcaacagg cgctgaacgg cagccgtcct tattattatt
     2581 ggctggaaga ggtggatatc tacggtcaca acaaggtata tggaccggtt gaggtgcgta
     2641 aaacaccctg acacggccca gccaaggaga gagaaacgcc tctcccgttt tcacgaagag
     2701 aagaagttgc gcgcgtggtt cgatacaaat aacccacagg ttcttgtgaa aaacgacgat
     2761 ttctcactgg ggcttcaact gttgttgctg taaccgtcat tcaaaggttg tcttatttgc
     2821 cttttgtaat tttttgctgt aatcccattt tgtaaatgat tggaccctgc agctcgctac
     2881 gcccataaat atcgaccgtt tccagccagt aataaaaagt tttatttgcc cccaattcga
     2941 ctggcagcaa acgtgcactt tcatccagga atttgtactc cgaaccgata agagagcctg
     3001 gttgagagct gggaatcatc tgttcattga tcctggtcct ggctccctca acagatgtcg
     3061 ctcttaacaa gttgaagccc aggttgttca cctcattggt ggtttcccat acaagcttaa
     3121 ccgctttttc ctctccgatc gctgtaaacg agagcaggtc cgccgaggtt ggatctccag
     3181 tcgcttcgaa agcgccaata tcgcaatgcg ggccctgtgg gcgcggcact cctcgctgat
     3241 caaacgggct gcaagtcgca tcattgccag catcaatggc agatgaccca accaggagcg
     3301 ggatcgtcca ggtcggtccg ccatagttgt ccggagggcc gaggagggga ttgctggaga
     3361 tgatgttcgt gcagacaccc ccagccgggc aaccgccttc aatcacgcta tcgttaatga
     3421 tcggcatgcc tgagtgaaaa ctataaattt gcgagccgct ggttaccgcc gtgtttcgcc
     3481 aaaaaataga attgttgacc ctgttgttgg tcgcatcggt tgccatgcca ccgccataaa
     3541 tcccggcagt gttggcagtc agcgtcgcgc catctaacaa caggctgccc gcagaattaa
     3601 aaatgccgcc accgtatgtt aatgctgagt tattataaaa tgtcgcgttt ttaagcaccg
     3661 ggtcactgga ataattatat aaacctcctc ctcgttcgga ggcggtattc cccataaatg
     3721 taatattcgt cattttgggg tcactattgt tgttgttcat ccccccgccg tttttcgctg
     3781 agtttccttc aaaggtgacg tctgttaatg tcgcatgagt ataggagttg taaataccac
     3841 ccccatgatt cggcgtggaa ttgccaatga accggaccgc ttgcaaggta ggattgctat
     3901 tcgaagtaaa aattccgccg ccgtaatctg tggctgtgtt accggtaaac gttacctggt
     3961 caagatggaa atcacaataa tcgacgttat acatccctcc gcccctatat acggctgagt
     4021 tatcggtgaa tgttacacgc agaagcgaag gactgctgta ttcccagtta tagatacccc
     4081 ctccataatt tgctgtgtta ttagagaagg tgacatcggt caaaatggga ttgctgccat
     4141 tattattcat ccccccgccg tatatttcgc ttttattccc tgtaaaatca acatggttta
     4201 aggttggttg gcagcctgag ttgaacatac caccgccatg ttctcgagca gaattgtttg
     4261 tgaacgtgac aaacgccagg ctcgggtcgc tcgaatagtt atacattccc ccaccgcgct
     4321 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgacaaacg ccaggctcgg
     4441 gtcgctcgaa tagttataca ttcccccacc gcgctccgaa gcggaattac gctcaaaaga
     4501 cacgttttcc aatgtgacat tgctgtggga cgaatttaac attccgccac cacccttggc
     4561 tgtattgccg gtaaaggtca cattttccag ttcaattgtt cccccgccgt tgtacatccc
     4621 gccgccactt gtgaccgccg agttttcagt gaatgagcaa tcagataatg aaacggtgct
     4681 caggtaatta ttcatcccac caccaaggtc ctggctggcg tttcgcacaa aatcgacacc
     4741 tgtcaggttt gaaccgctgg aataattaaa tacgcctcct ccccgttgaa cagctgaatt
     4801 ttcaacaaac aggacatttg tgaaagtcga gctgcctaca tagttgtata ccccacctcc
     4861 acggtcgccc gatgagttgt ttctgaattc tacatttatg aaagagggat tactgctgga
     4921 agcaaacacc ccacctccta atgaggccgc gtagttattg gtaaaaatca cattgcggat
     4981 aacaggacta cccttatagg cgtacaggcc gccaccacgg ttatatgggt tctcgccatc
     5041 agcgtaccct ttctcaatgg tgaagccatc caaaatggag gaaccgtccg cgccgctgcc
     5101 actgaccacg tgataactgt tgtctgtgtt gttacctggc acacccacat caccactgag
     5161 gaccgttata ttcaccgaag gatcgcgttg attcagctcc agctcagtac cgccaaaccc
     5221 gccataaatg gctactcctt ttttcatcac gaagctgctg gcgcgatcag ctgtggaggt
     5281 cggcaagtaa acacccgcgg caacccaaat ttggtcgcca ttggaagaaa ggctgattgc
     5341 gcgtggcagt gaacaagcat tctcccatgt ctcgccgcac gtaccgcttt caataccggc
     5401 aggttttaca cggataacca ttccatccgc atgtgcctgg ttcacggaaa taccacacaa
     5461 aatcagcgag aaaaagaaaa taaaagtgga tttaccaaat atttgcaatt tcatcgacac
     5521 acctcatcga cacaatcacc aataaaatta tgcagcaaat accttgtaat aaacttacag
     5581 aacgtttaaa gaacaaattt gttaagtatg gagtgtaccc aaagtgtccc cgaccgatgc
     5641 caggtctttc acgccgattt ctggcatttc tggctgcttc tgaagtgacg atctcgcgcg
     5701 aaattaagaa aaggacctca catcgctaca tggtaaaatg actgcggcga atactctcat
     5761 ccacaatacc tgtgaagatc cttccagcaa tcgagttcag caaccatcca tttcaaaact
     5821 gtcatcacgc ttgggggcgc cggaactcgc ttgtgcgcaa aatacaggta cttcactata
     5881 attattatga tcagccagtg cgtcatcgcg gctgggtgat catcgtttag accgctggaa
     5941 aaattccaaa tttcttctgt cagggaaatg tcaagaagga atttggatgc catgtgccat
     6001 catttggcat ttcacgatgt caaaagggcg gagcgcgcat catgcctgtc agcattcaac
     6061 aaaaacgaaa acttagaatc cttcttgctc tcccagtggc tgccatcctg ttatggggga
     6121 tcatcacccc gaacggtttt cgcgcagcgg ttgaggtgaa taagtggtcg ctggcgttta
     6181 tcgcgcattt ctatcagccc atttcttcag atcaagcatt acccttaccg cctgcgtctc
     6241 acccgcacgc gaagctgctt ctggcgcgtg aagcgattgc caatggtgat gacgacctgg
     6301 ctttagcgta tattaccccg ctcattggtc cggataaccc attggtaacg aatgcgtacg
     6361 cggaaatcac ctaccggcac gaggactttg ccagggcgat cagtgcctgg gaaatggcag
     6421 ggaatcttac agcgctgcac aacgccacct atgaattacg cgaaaagaag ttattagacg
     6481 acgcgttaaa ggccgccatc agccggtatc atcttgagcc tgaaacctct acttcagccc
     6541 tggcaaccat ttacactgat cttaaggaat acgaccaggc agtcgctttg ctcgatcaat
     6601 caatgggaga attccctgaa tccaaatttt ttgtcacctg gctgaacaca aaaagcggcg
     6661 ttcaattctt gcaggcaaat gactatgccg cgaataatca gttatctgaa gctgaattgt
     6721 tctatcgcgc atcggtgaat acgaacccga ataattggat cgcctggaaa aaatttggct
     6781 ggttctatta ctacaacttt aaagacagtg aatcagcagc caattgcttt aaaggagaaa
     6841 tggctgccta tcccgaaaac ggggaagggc aaatcgacct ggccactttg tacgctttag
     6901 aaaagcaagc gcaaccggcc ggctactggt acaggcaggc gatatccctc agaccagatc
     6961 gcggagattt tcagctgcat tacgcgaaat tcctgagaga ttcaaaggaa ttagatgaag
     7021 ccgccgaaat ctttgaccgc ctctcgcagg attttcctga ttacattgac gcgcattatg
     7081 aagccgcgtt gacctatgct caattgaatc aacccgccaa agccatccag tccattgaaa
     7141 gagtgttaca actgatgaac ccccctgtcc tcaggtacta cttactggct gggtcattat
     7201 atgaggcgaa cggcatgaaa gagaaagcgg ttgaagcctt taagaacgcg ttgctccttg
     7261 acccgcaaag ccccgaagcc ttgaatggaa ttaagcgact gtctgaataa atgagaagtc
     7321 aaatgccccg cagccgagcg aggttttcct ccataatact gacagataca cagaaggcgc
     7381 gatggtcagg ctacctgtat ggcgccatgc tgctcgtttg cgccgcgctt tggggatttc
     7441 tcatgatcta cgattacgtg cccttaatcg gttacgtggg gctctttttg ctcggggctg
     7501 gtaatgcgct catttgcagg cgtcttcctc gttccacccc gctggatgtg aatatcctcg
     7561 cgctgcttct cttgctccct gtgagcctgg cgctttctca caaccgcgag ctctcattgg
     7621 tgaaagttca cggcgtgctg ctctccattg cgctcttctg tattttggtc aattttctgg
     7681 ctaacttaaa aaggttgaag tgggcgatcc tggcgctcgc cgccacagcc gttttattgt
     7741 cagtgttggg cttctttggc gcggattggt cttctggaga ttgggcgctg cccgcaaaac
     7801 tccttgcctc cttgcccctg ccgttaaccg ccattcacca gttcaccgcg agcggcggga
     7861 ttcacgtgaa caccatcggg ggtactttag ccttgttcgc gccgctgctg atcagtctat
     7921 tgttagaccg cggcgcttac aggagggctt tccttcgcaa acacccgcgg gcgaaattgt
     7981 tcaccatcct ttataagttc atgatcgccg ccggaacagc gctcgtcttc ttcatgctca
     8041 tcctcacgca gtcgcgtgga gcgcttctcg gcactatgtg cggcgtgttt atcctgctgg
     8101 tgtggaaaga tagacgcttt ttatttatca tccccatcct ggtggtggcc ttattggttg
     8161 gcatatgggt ttttgccgat ggcgacccgg cccgattttt cctcctttta gatacctact
     8221 ccaaccctga aggcaacacg ctgcagatca ggatcgagta ctggaaaaac accctctatc
     8281 tgatccagga cctgccgatt accggcgccg gcatcggaac ctacggcaaa gtcttccatg
     8341 atatctaccc gttcgccacg cccctggcta tcgtgaggcc gctgttttac gcgcataata
     8401 tgtacctggc cgtggccgcc gatatgggtt tgcccgccct ggtgatttac ctggcgttgt
     8461 tcagcagttt ttttaccatg accctgatgg caatacgcaa agcccgctcc atcgtcaaaa
     8521 cgctgctgag aggcctgctt tgcggtatgt cggcccatat ggtttatggt ctcatggata
     8581 attatatgct gggcgaaaag ctcggttttt ccgtttggct ctttttcgcc gtttgcaccg
     8641 ccatctacat ccacagggaa accctggtcc actattacag cgaacaacca ggcgaggcgc
     8701 tgaacactac cccgcggctt ttcaggcagg tcgcgcggtc gcgcttacgg gatttatggt
     8761 ggaacctcgg cgcctggttg gtgatttcgg tcattggcat ttcattcatc ctcccccacc
     8821 ctcttatcag cctggtgatc acggtcagcg gcggtaccct gctgggaatt gtcatgacca
     8881 ggcgctttga attacaatat caaggagaac acgcgcgccc ggaggctcga acctcgcctt
     8941 agcgcgacca tccgctcaca tcctggacaa atatgggaag cacacctgat tctgattggg
     9001 acctgatcat cacgccgcgc aagaagtggt ttgaactgca gctgcaggag gtgtggaatt
     9061 accgcgattt gatcgccctc tttgtgcgcc gtgacttcgt ctcgcgctat aaacagacca
     9121 ttcttgggcc gctgtggttc ctcatccagc ccttgctgac ctccgtggtg ttcaccgtgg
     9181 tgttcggcaa catcgccggc ctgtccaccg acgccctgcc gcgattgctc ttttatatgt
     9241 ccggcaacgt gctgtgggcg tatttctccg gctgtctcaa cggaaccgcc accactttca
     9301 cctccaacgc gcagttgttt ggcaaggtgt atttcccacg cctggtaacc ccgctctcca
     9361 tcatcctttc caacctcatc acctttgcca tccagttcgt gttcttcctg ggtttcttcg
     9421 tttattacct gctgcgcggt tcgcctgtgc gctttaccct gtgggccttg tgccttccgc
     9481 tgctcatcct gctgatggcc gggattgggc tggggttcgg catcatcgtt tctgcgctca
     9541 ctaccaagta ccgtgacctg cagcacctgg tggtcttcgg tatttcgctg tggatgtata
     9601 tcaccccggt gatctacccg gtctcttcca taccagagaa ataccgctgg gtggcggtga
     9661 tcaatcccat cactcccatc gtcgaaacct ttcgcgccgg cttcctgggc gcgggcaacg
     9721 cctcgtgggg gatgctggga tacagcgcgg cattcgcggc ggtaattttg tttatcggcg
     9781 tggtcatttt caaccaggtg gaaaaaactt ttattgacac ggtgtaaagg gagaaacctg
     9841 ccattcataa gatgaactat gaatctcaaa ggaataatag tcacttgaaa cataattcta
     9901 accaacgaaa taaaaatgca tctcttttta agatggttgg gaatttttta tttgtttatt
     9961 tatgtttagt cctttttttt gtcctctcgc ttacactcgt atctttgata ccgggaacaa
    10021 agatcaagga aaatattgct tcatcagcgt taaccctaaa ggaacaaggc gatcgcccaa
    10081 gaatacttgg aaatcataag ttaaatcaat tggataactt aactgagagt gtaatgctag
    10141 gaattattta caacatcgat gagcatcaac ccctgaaatc tgctcttaag gcgatgtttt
    10201 atgctgggtc gaatagcaat gaagaaaatt accgtgtcaa ttcgctgtat gatcaaatcc
    10261 aatatgcctt ccgcccaaat aaccagtatc ctcgttactg gcatggatat atcgtcgcta
    10321 ttagaccgat gttaaccttc atgccatatt ctagcgtaca aaaaacgctt caaattgcct
    10381 tcttaatttt ttttgggtac ttggtcgcgt tgttttcaaa aaaactgtcg atgctggccg
    10441 cgctgtcctt tgcaataagc atagtcctgg ttaatatttc tataataccg gtcacagttc
    10501 atgcttcaat ggtatttttt atttgctttt ctgcaattgc tctttttctt aaaaccaaag
    10561 atatgtcgac agagaaatat atcattgcct tttttattat tggagcgctg actagttttg
    10621 tggacctatt gaccgctcct tttatcacat ttggctttcc agcgattgtt ttattaattt
    10681 ccaggaatac ggtcgagact cgaaattcct tcaaatcggt tttcagtttt gttgtttaca
    10741 tcggcctatc ttggatgatc ggctatgcct tgctctgggg aacgaaatgg gtgctggcca
    10801 gtattatgtt gaaagaaaat gtgattgctg aaggaataaa tggcattctt gaaagaattg
    10861 gccgtgacat tccttttggg aatatccagc atattaattc atttgatgcc attaagaaaa
    10921 acctgcaaac tttttatacc tttaccattt caaattaccc gatatcattt gtagtcattc
    10981 cactgatgat tttttatggt ttctttgccg taatatggca taaaaagaaa tatcgccttg
    11041 atacaacaat tattattgcg ttaattggtc tgctgccatt tttctggttc ggtgtcgcag
    11101 cgaaccattc atttatccat gcttacttca ctagccggat tttatcagtg tccgttttcg
    11161 cattctccta tgtcttaatc gatatagtgg attggaatca aatcaagaaa gcctacactg
    11221 cccttaagtc taagatgtct tcaaaccgaa aatctttaat agggatgttc ttcagaaaac
    11281 acaaggaaat acttttatga gacccgtcat ctccgttgaa catctcacca agcgctacga
    11341 gctgggcgtc atcggcaccg gcagcctcac ccgcgacctg gagcgctggt gggcgcgcgc
    11401 ccgcggccag ccagaccctt actcgcgcat cgggcaaaaa gaccccttcc gcctcgccgg
    11461 tcagtcgatc ctggcgttgg acgatgtctc tttccgcgtg gagcagggcg aggcgctggg
    11521 catcatcggg cgcaacggcg ccggcaagag cacgctgctc aaaattctct cgcgggtcac
    11581 cgcgccttcc agcggggtgg tgaaggtgaa agggcgcatc ggcagcctgc tggaggtggg
    11641 caccggcttt caccccgagc tcaccgggcg cgagaacatc tacctcaacg gcgccatttt
    11701 aggcatgaaa aaagccgagg tggcgcgcaa gttcgatgaa attgtcgact tctcaggcat
    11761 cgagaagttc atcgacacgc cggtcaaacg ctattccagc ggcatgtacg tgcgcctggc
    11821 cttcgccgta gccgcgcacc ttgaccccga aatactggtg gtggatgaag tgctggcggt
    11881 aggcgacgct gagtttcaaa aaaaatgcct cggcaagatg ggtaacgtgg ccagcgagga
    11941 ggggcggacg gtgctgtttg tgagccataa tatggcggcg attcagcagt tatgcacaaa
    12001 atcgatcttg atgaactccg gcaggcttga actggcgggc gattctctgc aagtcgtgaa
    12061 aaaatacctg gcttccaatg aagaaatggc agccgagaaa aaacatgcca tcttagccag
    12121 gaatgaacag gaaaattttc aattatccaa agtgttcttc gcggatgaaa ccgggtcaac
    12181 tcaaaacagt tttatgtcag gagaaagcat caccattcac ctggagatcg aatcggaaac
    12241 catttttcgc ggtttgatcg tcacaatcgg catcaatgac gaaaatggca caaggttgac
    12301 cgtcctgcat agtgatctct ccggtgtccc cctccaagtt accggcaagc ataccgtagt
    12361 aacctgcaac attccgaacc tgctcctgct gccgggtaaa tattacctgg atataaaaat
    12421 ctatcgtgga aaggatgtgc tcctttgggt gcccaacgcg aaaacgctgt tcgttgagcc
    12481 agggaatatt ctgaaaagcg gtaagctgcc cccaaaggaa tggggcggat tcttcgtttt
    12541 ggacaatcaa tggcaagcat cggaaaagac aacccgctag tttcggtcat tgtccccctt
    12601 cgcaaccgtt ttaacctggt cgatgaagcc gttgatgcgg tttacaagca gaattaccgc
    12661 ccaattgagt tgatcctggt ggatgattgc tccagccagg cctacaggcc aaaaattcaa
    12721 gcgcacgcgg gtttcacggt tgctgtgatt cggcaccaag agaaccaggg acccggcgcg
    12781 tcgcgggaaa ctggccggca gcgagcgact ggtgcctacc tcgcctacct tgattctgat
    12841 gacctgtggc atccaacctt cctggaaaaa atggtcttgc aactcaaaga gaagcccgaa
    12901 accgggatgt gctactgcaa gaccaggcag ttcagtggcg cggacctcaa ctcagggtct
    12961 ttgcgtcctc gcaacgaaca ggcatttgac gccatcctgc ccacattact ctttgggcgc
    13021 ccctggagca caggcgcctg tttatggacc aggagggctg ctgaccaaat cggtccatgg
    13081 tctgaaggct ggacgaacga ggacattgag tacgaaatcc gcgcaggcat cttcggcatt
    13141 caaatatccc acctgccaga agtgctgtgc tatgaaaggg aaacggacga tccccagcag
    13201 ttgtctcaag caacccacaa aaaggcgctg ctgcaaaaat tttcttcaat taaggcaatg
    13261 gccataaccg tgcttgaaaa caagcacgca cttgaagaat ccaccagggt attattcgtg
    13321 aaaaaaaaga tgcgccctat ggtcactgac ctgtttcaag cagaggaata cgagaaagta
    13381 atcgagcttt gtaagttgat gaacaaaatc gtgaagcctc tctcgaaagc atggttgctc
    13441 accgccctcc tgctggcaag cgtacggtat cgaaaattcc ccggcgccgc gaaaatcttt
    13501 cagctgggat gtcatttcct cacttgaaca agaaaaccac catcctcttc atcaccgctt
    13561 attacccccc gcatcacggc ggcggtttcg gcctgcgctg ccgtgatgtg gcggagggtc
    13621 tgcgccggcg cggtcactcg ctcaccatcc tcaccaaccg ctgcgcgctg cgcggctgct
    13681 gtgaacacca gagcgaggag cgaacattgc gcctgctggc gctcagacca gcgggagaca
    13741 acccgctgcg gcagatcctg tttgaccggc gcgaattatt gcaagtggcg cgcgtcgtcc
    13801 gccaggtgca gccgcgggtc atctacctct ggcacatgca gaacctgtcg aacgcgattc
    13861 tgccctttct cgccgggcag cccacgccgc tgatcctcga tgacggcggc tcggagctca
    13921 tttacctttc acggctgcag aaacgcgggt tgtacttcta caagaacgcg ggcgagccgc
    13981 ggctcaagac ctggcttaag cgcctggtta aaacgggcgc caggctggtg agcggcgggc
    14041 ggattgtgcc agattggcag tggcccgaag gcatgcgcgt gctcttcaac agcccttccg
    14101 cgctcgagca cgcccgcgcg cagggagggt tcgtgcgtaa cgcctcggtc atcccctcgg
    14161 ggttggatat cgcgcgcttc ccgttcaaag ccgcgccgcg aattcacacg ccggtgaaga
    14221 tcatcctgcc ggcgcgcatc aaagagcaga agggctgcct ggatgggatc ctgctggtgg
    14281 aggaactgcg ccggcgcggc gtgcccgccc aactgctcat catcggcgag gcgcagtccg
    14341 cggcctacct ggcgctgctg cggcgcgagg tggaggcgcg ccgcctgagc gggcaggtgg
    14401 acattcgccc catggcgggg caggatgaaa tgagccggct ttaccgcgag catgatttct
    14461 gtttcttccc ctcgtccttc aagaccggct tctcgcgcgt gccgctggaa gccatggcca
    14521 gcggctgcct gctgctcacc tatgggaatg aaggctcgcg ggaagtggtg caggacggag
    14581 caacgggcta cctgctgccc gagggagatg tggcagccgc cgcggaacgg gttcacgcgt
    14641 ccaccctcga ccacgcgggt taccttcgca tcctttccac cgcgcgccga caggtggaag
    14701 aaaggtattc actggaagat tacctgcaga aggtcgaagc gctgctcagc cgcgctggag
    14761 aaaccccgtc atgagaaacc cgcccggcag cagggcaacc tgcgttgtgc attatccttc
    14821 actgcgggtg atggcgggca acccctactg ggcgatgctt gaatcagggc ttaaggaggc
    14881 tggaataacg ctcctcgatg agccggatgc gcacaaactg cgctggctgt tcaaaaatcg
    14941 aaacagggtc gatatcctgc actttcatta ttttcaattt ctgtattgca accgcgggcg
    15001 cagccgcgcg cgccttgttt atgtggccag gttcgcgctc ttcctgctgc tcgcccgcct
    15061 gctcggctac cgcaccgtgc tgaccctgca caacctcaag cccacccatc cgctcaaccc
    15121 ggcctggatc gattacctgg ggcattggct ggcggcgaat ttcacccaac gcgtcatcgt
    15181 tcactgccat gaagccaggc ggcttttagc cgcgcgttat ggcaggaaaa acaacgtcat
    15241 tgaagtggcg catccacact atctcggcta cgcttccggc aagccctcca aaacagaggc
    15301 tcgcaaaatt ttgcgcctgc cgccgcgctc gccggtcttc ctcttttttg gcggcgtgcg
    15361 ccgtaacaaa gggctggaaa acctgattca aaccttcaaa gagatcgaag agcgcgagcc
    15421 cacactcctc atcgccggaa aaccagagcc cggcgctggg tacgccgagc agctgcaaag
    15481 cctggctgga aaagacccgc gcatcaggtt ccattttggc tatattccag atgaggagct
    15541 ggcgctcttt ttcaacgcga gcgacatcgt cgttttgccc ttcagcgcta tcctcacctc
    15601 cagctcaacg atcctggcga tgtcgtttgc ccgcgcggtc atcgccccgc gcatgggctg
    15661 cctgcctgaa ttgctggagc caggggccgg ctggttgtat gaagcggaca gccccggcgc
    15721 tttattgcaa acgctcggca ggtcgctgaa agaagattgg cgccaggcgg gggtcaccgc
    15781 cctggagcga gttgccagcc acaccccggc catcttcatc cgtgaaaccc tgtcgtgtta
    15841 tggctgcaca aacagctctg cgtaagtgct catgaacctt cccaccgtca gcatcgtcac
    15901 cccctgttac aacgccgccc gcttcatccg tgaaaccatc gagagcgtca gcgggcagcg
    15961 ttacccccat ttggagcaca tcgtcataga cggcggctcc accgacggca cgcttgacat
    16021 cctcaaagcg tacccgcatt tgcgctgggt ctcagcgccc gatagggggc aatcgcacgc
    16081 gctgaacaag ggattcgcca tggcgcgagg cgagctcatc ggctggctga acgccgatga
    16141 cacctacgcc cccggcgcgg tatcgaccgc ggtcgattac ctccttcaac atcccgaagc
    16201 gggcatggtt tgcagcgata tgtggatcgt cggtgaaaat ggaaagcccc tgcggctcgc
    16261 ccgcggcaaa ccatttgacc tggtcgagct gttaaccgag aactacgtgc ggcaggcgac
    16321 cgtctttatt cgccggcagc tgctcaatga agtgggcggc gtgaatgaat cgctgcacta
    16381 ctgcatggac cgcgccttgt ggttgagggt gggcgtgcat caccaggtcg cctatctgcc
    16441 gggggtcgtc agcgccaact tcaggttcta ttggggcagc aagacctcgc tgtatacacc
    16501 cgggttcgtg gcggaatggc tgggcgtgct caccgacgcg ctggataacc cgctttatca
    16561 acccattcca cgcgctgaag tgctcaaagc cattgaaaaa taccaggtgc ggcaccaggt
    16621 agcgcttatg cggcaggcag ccaggcaaaa agacccgcgc tccacctggc gcgcctgcgc
    16681 cgcgctcctc agccggcact ggcgctacct gttgaggtac ccttacctga aaacgcgggc
    16741 gctcctggca gctaaacgat gaacatcctc tacgtgcagg cggggcaggc tgtgggaggg
    16801 tcaaaggaaa gtctttacca gatcctccgg tcattgcccg atgaatttgc gcgccaggtc
    16861 gtgcttgaac gcggctcgga ccccggtttc aacgagcgcg cgcgccgcta cgcctcagcg
    16921 gtgcacctgc tgccgctgcc cacctggcag caataccgcc gccgcacgct ggctgaacgc
    16981 gccagggcgc cgctggcgga cgcctggcgg cttctgcggt gcgcgctggc cgccttcaaa
    17041 atcgcgcggg tcatccgcca ggagcggatc gacctggttc acaccaacaa cagcatgagc
    17101 ccctcgggcg cgctggcagc gcggctcacc gccaccccgc atatctggca tgtgcgggaa
    17161 tccattggga agcacaaaca ataccctttg ctgctgggca acctcgccct gcgcctgatg
    17221 cgccgttgga gcgacaccgt catctgcaat tctgattaca ccgccgaagt tttcagagaa
    17281 atgcgcatcc cggtgcgcgt catcccaaac ggcctggacc tggcggattt cgcccccgat
    17341 gcggggacac ctcgtgagct gggcagcctg gctgaccttc cgccgggcga aaaacgggta
    17401 ggcatggtgg gaaacctcac caccaaaatg aaacgacatg atgttttcct ggatatcgcg
    17461 gcgctggtca aagccgccgc gccgcgcacc a
//
LOCUS       DAOSPM010000038        17025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_3390_length_17025_cov_14.187979, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000038 DAOSPM010000000
VERSION     DAOSPM010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 17025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17025
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3390_length_17025_cov_14.187979"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..677
                     /locus_tag="PLW25_04850"
     CDS             156..677
                     /locus_tag="PLW25_04850"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOS53852.1"
                     /translation="MEINAKTKLHNLLSTYPQLEEKIIQAAPAFKNLKNPVLRRTVGQ
                     LATVEKVVQIGGLDLTTFINLLRREVGQPELIPDPSSDNGKDKSAPGAKPDWTNGKIQ
                     FTVDGKELLAKGEVPVNHVNALLPQLEENKLILLVTDFEPIPMIEVLKKQKREVFHMV
                     DSHDAGKHLTYIK"
     gene            999..2168
                     /locus_tag="PLW25_04855"
     CDS             999..2168
                     /locus_tag="PLW25_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOS53853.1"
                     /translation="MKKTALLLISLLVVFALALSACAPKPPVAEPPVVEEPAVEAPAV
                     EEPAAAVDPMAELVAAAQKEGNLTVIALPRDWCNYGEAIDTFKAKYGIAVNELNPDGG
                     SADEIEAIKANKDNKGPQAPDVIDVGYGFGPQLIEEGIVQPYKVSTWDSIPAESKDPD
                     GYWYGDYYGIMAFEVNSDVVEVPQDWADLLKPELKGQFALSGDPRASAQAQMSVYAAA
                     IYNGGGLDDVQPGLEFFKQVNDAGNFVPLIAKQATVAKGETPVILRWDYNALADKDFL
                     AGNPNIEVVYPASGTIAGIYIQAISAYAPHPNAAKLWMEFLYSDEGQNIWLKGYCHPI
                     RYADMQARGVIPAELSAKLPVFNGPVAFPSIDQINNAKMIIADNWMSVVGADVKE"
     gene            2155..3066
                     /locus_tag="PLW25_04860"
     CDS             2155..3066
                     /locus_tag="PLW25_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOS53854.1"
                     /translation="MSKNNSFLTDQKRDARKARPSSNSWRKWIGVVPFFIFALLFILL
                     PSVNILAGAFRTNAGRFTLANFAGLFEPSILSAYWVTIKISLTTALVGGLLGFLMAYS
                     VTVGKLPKAVRNMLMTFSGVASNFAGVPLAFAFIATLGRTGLITLILKNVLKLNLYAL
                     GFNLYSFWGLSLTYLFFQFPLMVLIITPALDGLKKEWSEAAENLGATRSLYWRKVALP
                     ILTPSILGAIILLFGNSFGAYATAYSLTGGFINLITIVVGSQIKGDILYNPGLGYALA
                     VGMVIVMAVMMAIYYYLRRKSERWLPR"
     gene            3063..3860
                     /locus_tag="PLW25_04865"
     CDS             3063..3860
                     /locus_tag="PLW25_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOS53855.1"
                     /translation="MKRGSFWSWLWVIIGTLYFLLPLISTFDFSLRAKKGELSFLAYT
                     RVFTDPNFWATMRFSLLIALFTIFLSIVLIAPTAYWIRLRLPKLRGIVEFISMLPYVI
                     PAIVWVFGAIRTFSGTPFFLTNTLFGTYVLLTTGYVVLSLPFVYRSIDVGLAAIDVKS
                     LTEAAQSLGAGWGTILFKVIFPNLKVAILSSSFLTLAIVIGEYTIASFLVGIKAYGPY
                     MSMIGQNKTYESSSLAIISFLLTWAFMGVIQLLTRGKTSGSSMAGAR"
     gene            3903..4952
                     /locus_tag="PLW25_04870"
     CDS             3903..4952
                     /locus_tag="PLW25_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53856.1"
                     /translation="MAFLTLKNVSKVFGNTTAVEDFNLEVEKGEFVSFLGPSGCGKTT
                     TLRMIAGFELPTSGIITLDGEDITYKSPNQRNVGMVFQSYALFPNLTIGQNIGFGLQV
                     RKMPKEEMEKRIDEMLSIVHLEAHKNKYPHQLSGGQQQRIALARALAINPRVLLLDEP
                     LSALDAKIRVELRLEIRRIQQEMGITTIYVTHDQEEALLISDRIVVMSQGLIEQVGTP
                     DKIYNYPSNEFVAQFVGQLNLLPITDVDLENGKCLLAGQEVRFEHTPGRIISESPRLA
                     IRPEELQIGTGEGRNALKGRVESALFLGAVVRLRVDIGGVFLSADFFNERSRTLPHSG
                     DEVVISFPEYSCWVM"
     gene            5142..6581
                     /locus_tag="PLW25_04875"
     CDS             5142..6581
                     /locus_tag="PLW25_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HOS53857.1"
                     /translation="MAETQTLLSVGIDVGTTTTQVVFSRLGITNAARTGLIPRMAITD
                     KEVLYQSPIVLTPLLDQDLVDAEKLVEIVRREYRAAGIEPAQVGTGAVIITGETARKK
                     NADAILHSLSGLAGDFIVSVAGPQQESLISGRGSGAAQYSREHYQMVTNLDIGGGSAN
                     CAVFKGGNLIGGATMNFGGRILTIDHTSGRVLHIAQAARYILQDCQLNLQTGDTPALA
                     DLRRFTDRMAEVTLELIEGGTSVLARQLYLTPPSQVSGAGSSLMFSGGVGHYYYHPVE
                     IRTLTDVTVHDDVGPLLAESLRNNAALSAYRVITPAETLRATVLGASTQTVSLSGSTI
                     WAEREILPLRNIPVLHLPVEPETQPSALAASLQALVRQNSQDPLEDQYAIALDLNSSL
                     DFAALQHLAAGIAEFAGSLPEQMPLLIIVERDYARVLGQTVKSLAPERPLLVIDQIGL
                     MEGDYIDIGTPLMDGRVVPISVKTLVFYN"
     gene            6641..8008
                     /locus_tag="PLW25_04880"
     CDS             6641..8008
                     /locus_tag="PLW25_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HOS53858.1"
                     /translation="MLLRTKLHGKTYEFPDVRLLMGKANEEKSGDRLAGVAAESAAER
                     IAARLVLAELPLWVLRQNPAVPYEQDEVTRVIQDAVDETVYAEIKDWTIGEFREWLLA
                     DTTTHEMIQRVSAGLTAEMVAAVTKLMSNLDLVLAASKIHVTAHCHNTMGAPGRLSSR
                     LQPNHPTDSPEGIRAEIYEGLAYGCGDSVIGINPSDDSYSSVSRLLEMTYDLIKEWEV
                     PTQNCVLAHVTTQMKCLQSGSPVGLVFQSISGSQKGCESFGINVGMLDEAYELAKKHC
                     FPSGPNFMYFETGQGSALSADAHNGWDQLTLEARNYGLARRYHPFQVNTVVGFIGPEY
                     LYDANQIQRAGLEDLFMGKLSGIPMGVDACYTNHARADQNANENLAVLLTAAGCNYFM
                     AVPMGDDSMLSYQSTSYHDTASLRQLFRLRPAAEFEHWLESIGLMKDGRLTELAGDPT
                     FFLKR"
     gene            8013..8981
                     /gene="eutC"
                     /locus_tag="PLW25_04885"
     CDS             8013..8981
                     /gene="eutC"
                     /locus_tag="PLW25_04885"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HOS53859.1"
                     /translation="MMMEEEKINQIVELVLRQLQEEGLVPGGLKNQPGQPTFPQETIT
                     TGAATVFHPGAALSTHAEIEIDLPDPAGDNLRWLPRVKNAKDPGALIELMASTSARIG
                     VGRAGPRSNTASLLLFQADHAITQDALYRDVDQRLLDEFNLMTVQTRITEGREEYLLR
                     PDLGRQLSDAARQMITEKCQKNPDLQLVVGDGLSALAIESNLRQIFPVIVQGAQSAGL
                     RMGTPFYVKYCRVGVMNDIGDILQPKVLILLIGERPGLGRAESMSAYMAYQPGAGQND
                     ANRDVVCNIFEKGGTNPLEAGAFIVQFAQKMMQYQASGVKLKLAAG"
     gene            8989..9648
                     /gene="eutL"
                     /locus_tag="PLW25_04890"
     CDS             8989..9648
                     /gene="eutL"
                     /locus_tag="PLW25_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HOS53860.1"
                     /translation="MAVLDKIQAKALSVQLIPRVEDNFARRLNLTPLQLSVGLITSNN
                     DDATYVAVDHATKMADVEVVYAHSFYAGSKHASGLLSGEIIAILAGPNPYEVNEGLKA
                     AVDYLETEAFWYSANEDASVAFFPHVISQTGSYLSRTNNLAVGTPIAYLVAPPIEGLV
                     ALDAALKAAAVSIVALTMPPSETNYMGAILTGEQPACKAAAQMFQEKVLDVVRNPFSG
                     I"
     gene            9679..10269
                     /locus_tag="PLW25_04895"
     CDS             9679..10269
                     /locus_tag="PLW25_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOS53861.1"
                     /translation="MELTKPITYFLTTGEVNTTRTFELARARALELGLKTVLVASTSG
                     KTGALAAEFFKGFEVVVVSHSAGFREDNLQELTSDSRQKIEGLGGKVLTATHAFGGIN
                     RAVRRKFNTYQVDEIIAGTLKILGQGLKVAIEIALMAADGGAVRTDKPVLCIAGSNAG
                     ADTAVVLIPAYSQSFFDIRLLEVICLPSPEHREFQK"
     gene            complement(10464..13295)
                     /locus_tag="PLW25_04900"
     CDS             complement(10464..13295)
                     /locus_tag="PLW25_04900"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOS53862.1"
                     /translation="MESPAILDLTIGFHALDSSEVEQKLQTKLTCGLTSEEAARRLAH
                     FGKNELTVKKRTSFLQRVLNALNNFVVILLLVAAIISALLGDYIESAVIMAIVILNTI
                     LSVVQESRAEEALAALKKMAAPEASVLRDGHRVNIPAPELVPGDVVFLESGNFIPADL
                     RLVESINMRIDEAALTGESVPVQKNASFRLENNIPLGDRKNTAFMGTTVTYGRGMGIV
                     IGTGMKTQLGMIADMLQSVEEEVTPLQKRLDSLGKVLGWASLGICALVFVVIVVRHLL
                     GPEGSSSLIKTLVDGFMIAVSLAIAAVPEGLPAVVTISLAMGMREMVRRHALIRKLAS
                     VETLGSATVICSDKTGTLTQNEMTVTRIWVDGMDFEITGTGYTPTGDFLYAGEKITVE
                     DYPGLATTLWVGVLNNDAYLETVSDAGGESYHLIGDPTEGSLVVAAAKAGVILGELKG
                     KYPRENEVPFDSTRKRMVTIHSTRGVQFNDASPIHDESKKDWYAIIVKGAPDVVLNLC
                     SKYTQIDDKEILLNEEVRSQILEANDCLTRDALRVLGLAYKLVPNAPKVNDTELDIED
                     LERDLVFVGMMGMIDPARPEVNPALEKARTAGIRTIMITGDYPNTAAAIAKSINLLLP
                     GHTVRTGAELNLLSAEEIREAVKTTDVFARVSPEHKMKIVEALRANNQVVAMTGDGVN
                     DAPAIKRADIGVAMGITGTDVAKETADMVLTDDNYASIVSAIEQGRIIYSNIRKFVYY
                     LISCNIAEILIIFLSTLFTGKSPLTAIQLLWLNLVTDGAPALALANETGDPDIMEQQP
                     RPPNESIINKYMRGGIIVQTIAITSAVLLAYFIGLRAHPELPEFAETMAFTTLSISEL
                     LRAYTSRSEYFPVLKIGLFKNKWMNVGVFISLMLVFLVIYVPFLNTLFNTVPLGWAQW
                     EIILPLILIPSVAAEGKKYLERMLKKRSA"
     gene            complement(13941..16355)
                     /locus_tag="PLW25_04905"
     CDS             complement(13941..16355)
                     /locus_tag="PLW25_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOS53863.1"
                     /translation="MNLRAKAREIVAQMILEEKSSLCSGKDYWYLKPIDRLGLPSIMV
                     TDGPHGVRKQLSSSEDLGISENVPAVCFPTAVSMACSFDPALLREVGQSLAEECLQEG
                     VSVLLGPGMNIKRSPLCGRNFEYYSEDPYLSGHLAAAFIEGVQSKGVGTSLKHFAANN
                     QEKRRLTVDAVMDQRTLREIYLSGFEFAIKQARPWTVMCSYNRLFGEFVSQNEYLLTQ
                     ILRQEWGYEGLVISDWGAVVDRVKGVAAGLDLEMPHLDRTHDERVAQAVIAGDLPVAL
                     LNQTAENVTALLLRARGQKRAQYNVSAHRALARRAAAESAVLLKNEGNLLPLNPSSSV
                     ALIGAFAKNPRYQGSGSSRITPIRVNDAVEELKSLGLRFEYAAGYFEDADAPDEGLLA
                     EACQVAAGKDVVLLFVGLPDRFESETFDRETLALPQSHERLIEAVSEVNHNVVVVLYC
                     GGVVEMPWADRVKSILLMHLGGETVNGAVADLLLGRVNPSGKLAETWPFQLEDNPSFN
                     YFPGYPLTVEYREGIFVGYRYYDSAHVPVRFPFGFGLSYTTFSLSDLQLSSNKMGDSD
                     TLKVTCRVSNTGSRAGSEVVQLYIAKKGSVIIRAEQELKGFIKARLSPGESQQVEFTL
                     FQRDFAYYNVNLADWHVEEGEYEVRIGTSSRDIQLKAVLTCTTRVKAALPDLRAQAPG
                     YYHPSARWTVSDADFSTILGRSIPARERVKGAPHTVNSTITDIQDKWFGRLIRHLINQ
                     QVTKIGAKDPYLKMMAEKIVNDMPLRFLTMMGSDSMSILQVEGLVDVLNGHFLKGIKK
                     LRKIAK"
BASE COUNT         4017 a         4361 c         4500 g         4147 t
ORIGIN      
        1 tctattatgc ggtgcgcgat gagcaaggag gttaccttgg cacattggag gtaacgcagg
       61 acgttacgga tattcgcgca ttagaaggcg aacgccgcct gctggaatat gatggagcac
      121 cgaaataggg aagagacgaa aatcaggaga aagacatgga aataaacgcg aagaccaaac
      181 tgcataacct attgagcact tatcctcaat tagaagaaaa aatcatccag gctgcgcctg
      241 catttaagaa cctgaagaac cccgtcttac ggcgcacggt tggacaattg gcgacggtgg
      301 aaaaagtggt gcaaattggc gggcttgacc tgacgacttt tatcaacctt ctccgccgtg
      361 aagtaggaca acctgaattg atcccggatc cttcctcgga taatggaaaa gacaaaagcg
      421 cgccaggcgc taaaccagat tggactaacg gtaagattca gttcactgtg gatgggaaag
      481 aactgctcgc aaaaggtgaa gtgccggtaa accacgtgaa tgcgctgctg ccacagctgg
      541 aagaaaacaa gctcatcctc ctggtgacag attttgaacc catcccaatg atcgaggtgt
      601 tgaaaaagca aaaacgggaa gtgtttcaca tggtggattc gcacgacgcg ggaaaacacc
      661 tgacgtatat aaaataaata tggagacgct tcagcccatc cgctgtattt gagataacac
      721 attgaaacaa taggccggta aacggcctgt tgtttttttt atacctcact ggggcatcca
      781 accaagcatt ccctcaaacc atgaaattga aatccatctt cttaaggtta ttgggttggc
      841 aaaacttgat tgtaattttc ttgtgttact atataatatg tctatcttca tcattaagcc
      901 gtttcacaag aagggaggtt tccggtcatt gtctctgtaa cacaacctgt tttgaagaaa
      961 gcaaatgtta caaaaaacaa aaacctgagg agagaaacat gaaaaaaacc gcattactct
     1021 tgattagcct gctggtggtc tttgcgctgg cattatcagc ctgcgcgcca aaaccacctg
     1081 tcgcagagcc gcctgttgtt gaagaacctg ctgtcgaagc acccgccgtt gaagaacctg
     1141 cagcggctgt agaccccatg gctgagttag tcgcagcggc tcagaaagaa ggcaatctga
     1201 ccgtgatcgc cctccctcgt gattggtgca actacggcga ggctattgac acgtttaaag
     1261 ccaagtatgg aatcgcggtc aatgagctga accctgacgg cggttctgct gatgaaatcg
     1321 aagccattaa agccaacaag gataataaag gcccgcaagc ccctgatgtg attgacgttg
     1381 gatacggctt tggcccgcag ttgatcgaag aagggattgt gcagccttac aaagtctcca
     1441 cctgggattc cattccggct gaatccaaag atcccgatgg ctactggtat ggtgactatt
     1501 acggcattat ggccttcgaa gtcaattcag atgtcgtcga agttccgcag gattgggctg
     1561 acctgttaaa accggaacta aagggtcagt tcgccctctc tggagatccg cgtgcttcag
     1621 cacaggctca gatgagcgtc tacgccgcgg ctatatacaa tggcggcggg ctggatgacg
     1681 tgcagccagg gttggaattc tttaagcagg tgaacgatgc gggtaatttc gttcctctga
     1741 ttgccaaaca ggcgactgtt gccaagggcg aaaccccggt tatcttgcgc tgggactaca
     1801 acgctttggc ggataaggac ttcctggctg gcaacccgaa cattgaagtt gtctacccgg
     1861 cgagcggcac aatcgcgggt atctatatcc aggcgatctc ggcttacgct ccgcacccca
     1921 acgctgccaa gctttggatg gaattcctgt atagcgacga gggacagaac atctggctga
     1981 aaggctactg ccatcctatt cgttacgcgg atatgcaggc tcgcggcgtg atcccggctg
     2041 agttatctgc gaaactcccc gtcttcaatg gtccagttgc gttcccgagc attgaccaga
     2101 tcaacaatgc gaaaatgatc attgcagaca attggatgtc cgtcgtcggc gctgatgtca
     2161 aagaataact ctttcttaac tgatcagaaa agggacgcgc gaaaagcgcg tccctccagc
     2221 aatagttggc gtaaatggat cggggttgtc cctttcttta tttttgcgct cctgttcatc
     2281 ctgctccctt ccgtgaacat tctggctggc gccttccgta ccaatgccgg gaggttcacc
     2341 ctggccaact tcgccgggct ctttgaacca tccattttga gcgcctattg ggtgacgatt
     2401 aagattagcc tgactaccgc gctcgttggt ggcttgctgg gtttcttgat ggcctattct
     2461 gtcactgtgg gtaaactgcc caaagcggtt cgtaacatgc tcatgacctt ctcaggcgtt
     2521 gcctccaact ttgctggtgt ccctctggcg tttgccttta ttgccacttt agggcgaacg
     2581 ggtcttatca ccctcatcct caaaaatgtg ctgaaactga acctgtatgc gctcgggttt
     2641 aacctttaca gtttctgggg actgtcgctc acgtatttgt tttttcaatt tcccctaatg
     2701 gtcctcatta tcaccccggc actggatggg ttgaaaaaag aatggagcga ggcagccgaa
     2761 aaccttggcg ctacccggag cctttactgg cgcaaggtcg ctctaccgat cctcactcct
     2821 tccattttag gcgcgatcat tcttttattt ggtaattctt ttggcgctta cgctaccgct
     2881 tactctctca ccggaggttt catcaacttg atcaccattg tggttggctc acagatcaag
     2941 ggtgatattc tctacaaccc gggcctgggt tatgcgcttg cagttggcat ggtgattgtg
     3001 atggcggtta tgatggcgat ttattattac ctgcgccgca agagcgaaag gtggttgcca
     3061 agatgaaacg cggatctttt tggtcttggt tgtgggtcat tattggaact ctttattttc
     3121 ttctccctct catttcaaca tttgattttt cgctacgcgc taaaaaagga gaattatctt
     3181 ttctcgctta tacgcgcgtt tttacagacc ccaatttttg ggcaaccatg cgtttttccc
     3241 tgctgattgc cttgtttacc atctttctga gcatcgtcct tattgctccc actgcctact
     3301 ggattcgttt gcgattgccg aaattgaggg gaatcgttga gtttatttct atgctgcctt
     3361 atgtgatccc agcaatcgtg tgggtttttg gcgccattcg aactttcagc ggtactcctt
     3421 ttttcttgac gaataccctt tttggcacct acgttttact tactaccggc tatgtcgttt
     3481 tgtcattgcc gtttgtttat cggtcaattg atgttggact ggcggcgata gacgtgaaat
     3541 cgcttactga agccgcgcag agccttgggg ctggctgggg aaccattctg ttcaaggtca
     3601 ttttccccaa cttgaaggtc gccatcctta gcagctcttt tctgacgctt gcaattgtca
     3661 ttggcgaata caccattgcc agcttcctgg ttgggattaa agcctacggc ccgtacatgt
     3721 caatgatcgg gcagaataaa acctatgaat cttcttccct ggccatcatc agcttcttgc
     3781 tcacctgggc ctttatgggg gtgattcagc tactcactcg cggaaaaact tctggcagct
     3841 caatggcagg tgccaggtga tatatagcaa aaacaataaa ccttacggaa aaatggtgac
     3901 ttatggcatt tcttactctt aaaaacgtgt cgaaagtttt tggtaacacg acagcggtgg
     3961 aagactttaa ccttgaggtt gaaaaaggcg agttcgtttc atttctcgga ccttcaggat
     4021 gtggaaagac cactacgctg cgaatgattg cgggttttga gttaccaaca agtgggatta
     4081 tcacacttga tggggaagat attacctata agtcgccgaa tcaacgcaat gtgggcatgg
     4141 ttttccaatc ctatgcattg tttcccaatc tgaccattgg gcagaatatc ggttttggtt
     4201 tacaggtgcg caaaatgccc aaggaagaga tggaaaaacg catcgatgaa atgttatcca
     4261 ttgttcatct tgaggcccat aaaaacaagt acccgcatca actctcgggc gggcagcagc
     4321 aacgcatcgc attagccagg gccctggcga tcaacccacg cgtactgtta ctggacgagc
     4381 cactttccgc gcttgacgcg aagatcaggg tggagttaag gctggaaatt cgccgtattc
     4441 aacaggaaat gggtattacc acaatttatg tgacccacga ccaggaagaa gccttgctta
     4501 tttctgatcg gattgtggtc atgtctcaag ggcttattga gcaggtgggc acgccggata
     4561 aaatctacaa ttacccctct aatgaatttg tcgcgcagtt tgtgggtcag ctgaacctcc
     4621 tgccaatcac ggatgttgat ttagaaaatg gaaagtgcct gctcgccggt caggaagtga
     4681 ggtttgaaca cactcccggg cgcattatca gcgaatctcc caggttggcg attcgccccg
     4741 aggaactaca aataggaacc ggcgagggaa ggaatgccct gaaaggacgg gtggaatcgg
     4801 ccctcttttt aggcgccgtg gtaaggttgc gtgttgatat cggcggcgtc tttctctctg
     4861 ctgacttctt caatgaacgg tctcgcactt taccccactc cggtgatgaa gtggtcatca
     4921 gcttccctga gtactcatgc tgggtgatgt aaagagttac tgaaagcaca taaaagagag
     4981 aaacagcctt ttatcccagc cgggatggca aaatgaactc aaatttattg agaaatgatc
     5041 tggcgagatt gggcgatcat cacacgataa acccgcccga ggttggaaat ttctgagttg
     5101 ataaaaataa tgaatatttc catttggtta gaataaaccc tatggcagaa acccagaccc
     5161 tgctctcggt tggcattgat gttggcacga ccactacaca ggtggttttt tcgcgcttag
     5221 ggattacgaa cgcagcccgc acgggtttga taccgcgcat ggccattaca gataaagaag
     5281 tgctgtacca gagcccgatt gtgctgacgc cgctgctgga ccaggatttg gtggacgcgg
     5341 agaagctggt cgaaatcgtg cgtcgtgaat atcgagcagc cgggattgaa cccgcgcagg
     5401 taggcaccgg ggcggtgatc atcaccggag aaacagccag aaagaaaaac gctgacgcca
     5461 tcctccattc actttcgggg ctggcgggcg atttcattgt tagtgttgcc ggcccacagc
     5521 aagaaagcct gatttctggc cgaggatcag gagcagcgca gtactccagg gagcactatc
     5581 agatggttac caacctggat atcggtggag ggagcgcgaa ttgcgcggtt ttcaagggag
     5641 gaaacctgat aggcggcgcg acgatgaact ttggtggaag aatcctcacc atcgaccata
     5701 ccagcgggcg cgtgttgcac attgcccagg ctgccaggta tattttgcaa gattgccagt
     5761 tgaacctgca aacaggagac actcctgcat tggctgactt gcggcgcttt acagaccgta
     5821 tggcggaggt tacgctcgaa ctgatcgaag gcggcacctc tgttttagcc cggcagttat
     5881 acctcacccc tccttcacaa gtttcaggcg caggatcgag cctgatgttc agcggcgggg
     5941 tcgggcatta ctattaccat cctgtggaaa taagaaccct gacagacgtg accgtgcacg
     6001 acgatgtggg ccccctgctg gctgaaagct tgagaaacaa cgctgctctt tcggcatacc
     6061 gggttatcac gccggctgaa acgctgcgcg ccaccgtgct gggggcgagc acgcaaaccg
     6121 tctcgctttc tggttccacc atttgggctg agcgtgaaat ccttccgctt cggaatatcc
     6181 cggtgctgca tctgcccgta gagcctgaga cccagccctc cgcgctggcc gcaagcttgc
     6241 aggccctggt gcggcagaac agccaggacc cattagaaga ccagtacgct attgcgctgg
     6301 acctgaactc cagcctggat tttgctgcgc tgcaacacct ggcagccgga attgctgagt
     6361 ttgctggcag cttaccagag cagatgccgt tgctcatcat tgtagaacgg gattatgccc
     6421 gggtactggg gcaaacggtg aaaagcctgg cgccagagcg gccgctgctg gtgatcgatc
     6481 agatcggttt gatggaaggc gattatatcg atatcgggac gccattgatg gatgggcgag
     6541 tggtacctat cagtgtgaaa acgctcgttt tttacaatta gaccaatctg ttgattcatt
     6601 ttataatcaa ggtaccagta cctcaataag cggagcgcgg atgctgttaa gaacaaaatt
     6661 gcacggaaag acctatgaat tccccgacgt gcggctctta atgggcaaag ccaacgagga
     6721 gaaatcaggc gatcggctgg ctggcgtggc ggctgagagc gcggctgaac gtatcgccgc
     6781 ccgcctggta ctggctgaac tgccattatg ggtgttacgt cagaacccgg cggttccgta
     6841 tgaacaggac gaggttaccc gtgtgatcca ggacgcggtg gatgagacgg tttacgcgga
     6901 gatcaaagac tggaccattg gcgagttcag agagtggttg ctggcggata cgaccacgca
     6961 tgaaatgatt cagcgcgtct ctgcaggcct gaccgccgaa atggtggcag cggtcaccaa
     7021 attgatgtct aacctggacc tggttctggc agccagcaag atacacgtca ctgcgcattg
     7081 ccacaatacg atgggcgcgc cagggagact ttccagccgg ttacagccta accatccgac
     7141 tgactcgccc gagggcatcc gcgctgaaat ctatgagggg ctggcgtatg gttgcggtga
     7201 ctctgtaatt ggcatcaacc cttcggatga ttcatattct tcggtctcgc gacttttaga
     7261 aatgacctat gacctgatca aggagtggga agttcccacc cagaattgtg tgctagcgca
     7321 cgtcaccacg cagatgaaat gcctgcaatc tggatcgccg gttgggctgg tgttccaatc
     7381 catctccggg tcacaaaaag gctgcgagtc atttggcatt aacgtgggca tgctcgatga
     7441 ggcttatgaa ctggcgaaaa agcactgctt cccctcagga cccaacttta tgtactttga
     7501 gaccgggcag ggatccgcac tatccgctga tgcgcacaac ggctgggacc agttgacgct
     7561 ggaagcgcgc aattacgggt tagccaggcg ctatcaccct tttcaggtga acactgtggt
     7621 tggttttatc ggtcccgagt atctgtatga cgccaaccaa atccagcggg ccgggctgga
     7681 ggatctcttt atgggtaaac tctcgggtat tcccatgggt gtggatgcct gttacaccaa
     7741 ccacgcgcgc gccgaccaaa acgccaacga aaaccttgcg gtactactta ccgcggcggg
     7801 ctgtaattac ttcatggctg tgccgatggg agatgactcg atgctctctt atcaatccac
     7861 ctcctaccat gacacagcct cccttcgtca gttattccgg ctgcgcccgg ccgctgaatt
     7921 tgagcattgg ctggaatcga ttggtttgat gaaggatggg cggctaactg aactcgccgg
     7981 cgaccccacc tttttcttaa agcgatgagc gaatgatgat ggaagaagag aagatcaatc
     8041 agattgttga acttgtactg cgccagttgc aagaagaagg gctggtgcca ggcgggttaa
     8101 aaaatcaacc cggtcaaccc acttttccac aagaaaccat tacgaccggt gcagcgacag
     8161 tatttcaccc aggcgctgct ttatcaaccc acgcggaaat agaaattgac ctgcccgacc
     8221 ccgctggtga taacctgcgc tggctgccgc gagtgaaaaa tgccaaagac cccggagcgt
     8281 taatcgaact gatggcatca acctccgccc gcatcggcgt aggaagggca gggccgcgca
     8341 gcaacacagc ttccctgctg ctgttccagg cagaccatgc catcacccag gacgcgctgt
     8401 atcgtgatgt tgaccaacgc ttgctggacg agtttaacct gatgaccgtg cagaccagaa
     8461 tcacggaggg cagagaagaa tatctgctgc ggccagatct tgggcggcaa ttgagtgacg
     8521 cggctcgcca gatgatcacg gaaaaatgcc agaagaaccc tgatctccag cttgtggttg
     8581 gcgatggctt gagcgccctg gcaattgaga gcaacctgcg gcagattttc ccagtcattg
     8641 ttcaaggagc ccagtcagcc ggtttacgca tgggcacacc gttttatgtg aaatactgcc
     8701 gtgtgggtgt gatgaacgat atcggtgata ttcttcaacc gaaggtcctt atccttctta
     8761 ttggtgaacg cccgggactg ggcagggcgg aaagcatgag cgcctacatg gcctatcagc
     8821 ccggggcggg gcagaacgat gcaaaccgcg atgtggtctg taatattttc gaaaaaggcg
     8881 gcaccaaccc gctggaagcc ggggcgttta ttgtgcagtt cgcgcaaaaa atgatgcaat
     8941 atcaagcttc cggtgtcaaa ctgaaactgg cggcagggta gggagcgtat ggcggtcctt
     9001 gacaaaatac aggcgaaagc gttatccgtg cagcttattc cgcgcgtgga agataatttt
     9061 gcgcggcggc ttaacttaac ccctcttcag ctttctgttg ggctgatcac ctcgaataac
     9121 gatgatgcca cttacgttgc cgttgatcat gctaccaaaa tggcggacgt ggaagtggtg
     9181 tacgcgcact ccttctacgc tggatccaaa cacgcttcag ggttgctttc aggcgagatc
     9241 attgccattc tggcagggcc caacccgtac gaggtgaatg aagggttgaa agccgcggtg
     9301 gactaccttg aaacggaagc cttctggtac tctgccaatg aagacgcttc tgtggctttt
     9361 ttcccgcacg tcatctcgca gaccggttcg tatctttctc gcaccaataa cttagccgtc
     9421 ggcacaccga tcgcttacct ggtcgccccg ccaatagagg gactggtggc gctagacgca
     9481 gccctgaaag ccgcggctgt ttccatcgtt gcgcttacga tgccaccttc tgagaccaat
     9541 tacatgggtg ccattctcac cggcgagcag ccagcctgca aggcggccgc gcagatgttc
     9601 caggaaaagg tgctggatgt ggtcagaaac ccgttcagtg gcatttaaca ataaaaaaac
     9661 agacgacaag gtgacaggat ggaactgacc aaaccaatca cgtatttttt gactaccgga
     9721 gaagtgaata ccacgcggac atttgaactt gcccgcgccc gcgcgcttga actggggttg
     9781 aaaacagtac tagtggcttc gacttcggga aagactggcg cccttgcggc ggagtttttt
     9841 aaaggttttg aagtggtggt cgtttcgcac tctgcaggtt ttcgagaaga caacctgcag
     9901 gaattgacca gtgactcccg tcagaagatc gaaggtcttg gcggaaaggt tctcaccgct
     9961 acgcacgcct ttggcggtat aaaccgggcg gtcaggcgta aatttaacac ctaccaggtc
    10021 gacgagatca ttgcgggtac gcttaaaata ttggggcagg ggttgaaagt ggcgattgag
    10081 atcgcactca tggctgcgga tggcggcgcg gtgcgtacgg ataaacctgt actgtgtatc
    10141 gctggcagca acgccggcgc ggatactgcg gttgtgctta tccctgctta ttcacagtct
    10201 ttttttgata ttcgcttgct ggaagtgatc tgtttgcctt cgccagagca tcgcgaattt
    10261 caaaaataaa accatcaatg gttgcaccct ctatgattta aacctgaacg ttcacgcacc
    10321 aggagagcca cgcaaggacg cgaggggatg gattgcggct tcagcggttt tgtttattgc
    10381 actcaagcgc gcgtttccta accggcatca tcaccactgg ggcaggtggt tcgcgaatct
    10441 gatttaatct cccaaccgaa acctcaagcg cttcttttct ttaacattct ttccaggtat
    10501 ttcttgccct cggcagccac tgatgggatc aggatgagcg gaaggatgat ctcccattgg
    10561 gcccaaccca gcggaacggt attgaagaga gtgttcagga agggcacata aatcaccagg
    10621 aagacgagca tcagcgagat gaacacaccg acattcatcc atttattttt gaataatccg
    10681 attttgagca ccgggaaata ttctgagcgt gaagtgtaag cgcgcagcag ttctgagata
    10741 gaaagagttg taaaagccat tgtctcagca aattcaggca gttcggggtg agcgcgcaga
    10801 ccgataaaat aggccagcag aactgcggag gtgatggcga tcgtctgcac gatgatcccg
    10861 ccgcgcatgt atttattgat gatggattca tttggcggtc ggggctgttg ttccatgatg
    10921 tcagggtcac cggtttcgtt ggcaagcgcc agcgccgggg cgccatcggt caccagattc
    10981 agccataaca actggatggc tgtgagcggt gatttaccgg tgaacagagt ggagaggaag
    11041 atgattaaga tttccgcgat attgcacgag atcaagtaat atacgaactt gcggatattg
    11101 ctgtaaatga tgcgtccttg ctcaatcgcg gaaacaatgc tggcgtagtt atcatccgta
    11161 agcaccatat cggccgtttc tttggccaca tctgtaccgg tgatccccat tgccacgcca
    11221 atatctgcgc gtttgatcgc cggggcgtcg ttcactccat cgccggtcat ggcgaccacc
    11281 tggttgttgg cacgcagcgc ttcgacaatc ttcatcttat gctctggcga gacgcgggca
    11341 aagacgtctg tggtttttac ggcttcgcgg atctcttcgg cgctcagcag gttcaattct
    11401 gcgcccgtgc gcacagtatg cccaggtaaa agcaggttga tggatttggc gatggcagcg
    11461 gcggtgttgg ggtaatctcc tgtgatcata atggtgcgga tacccgctgt gcgcgccttt
    11521 tccagcgccg ggttgacttc tgggcgtgcc gggtcgatca tccccatcat gcctacgaac
    11581 accaggtctc gttcaaggtc ttcgatatcc agctcagtgt cattgacttt gggcgcgtta
    11641 gggacgagct tgtaagccaa acccagaacc cgcaacgcgt ccctggtcag gcagtcgttc
    11701 gcctctaata tttggctgcg tacttcttca tttaaaagta tctctttatc atcaatctgc
    11761 gtgtatttac tgcatagatt cagcacaacg tctggcgcgc ctttaacgat aatcgcgtac
    11821 cagtcctttt ttgattcatc atggatcggc gaggcgtcat tgaactgcac ccctctggtg
    11881 gaatgaatgg tgaccatcct tttgcgcgtt gagtcaaaag gaacctcgtt ttcgcgcgga
    11941 tattttccct taagttcgcc caggataacc cccgcttttg ccgctgctac aacgagcgac
    12001 ccttcggtcg ggtctcctat gagatggtag ctttcaccac ccgcatcgct gacggtctcg
    12061 aggtatgcgt cgttatttag cacgcctacc cacaaggttg tggccagccc tgggtaatcc
    12121 tcgactgtta ttttttcccc tgcgtaaaga aaatcccccg tcggcgtgta accggtgccg
    12181 gtaatttcaa aatccatccc atcgacccag atgcgggtaa ctgtcatttc attttgggtg
    12241 agcgtgccgg tcttgtcaga gcaaatcacc gttgcggaac cgagggtttc aacggaagca
    12301 agtttgcgga tgagcgcgtg ccggcgcacc atttctcgca tacccatggc caggctgatg
    12361 gtcaccacgg caggcagacc ttcaggcacc gcggcaatcg ccaggctgac agcgatcata
    12421 aacccgtcca ccagtgtctt gatcaggctg gatgaaccct ccggaccaag caggtggcgc
    12481 acgacaataa ccacgaaaac gagcgcgcaa atgcccagcg aggcccaccc cagcactttg
    12541 ccaagcgaat ccaggcgttt ctgcaagggg gttacttcct cttcaacgct ttgaagcatg
    12601 tcggcgatca tccccagctg tgttttcatg cctgtgccga tgacgatgcc cataccccgc
    12661 ccgtatgtga ccgtggtgcc catgaaggcg gtgttcttgc ggtcccccag ggggatgtta
    12721 ttttccaggc ggaaagacgc gttcttttgc accggtactg attcaccggt aagtgcggcc
    12781 tcgtcaatgc gcatattgat ggattccacc aggcgcaaat ccgcgggaat gaaattacct
    12841 gactccaaga aaaccacatc gcccggaacc agttcagggg cgggaatatt gacgcgatga
    12901 ccgtcacgca acacagaggc ctctggcgcg gccatcttct tcaacgcagc cagcgcctct
    12961 tcggctctgc tttcctgcac gacacttaaa atagtgttga ggataacgat ggccatgata
    13021 actgcggatt caatatagtc gcccaggagc gcggaaatga tggctgcgac cagcagcagg
    13081 attacaacaa aattattcaa cgcgttgagc acgcgctgca ggaaggaagt tctttttttt
    13141 acagtgagtt cgtttttacc aaaatgagcg agtctccgcg cggcttcctc tgaggtcaaa
    13201 ccacaggtta acttcgtttg taatttttgt tcgacttcgg aggaatcgag cgcgtgaaaa
    13261 ccaatggtta aatcgagaat tgcaggagac tccatatcca atactccaaa ttttcaatgg
    13321 aacggtggtg aaatcccata aatttcgatg aaagggggtc aactttctgg tagacaccct
    13381 gcagcattgg gggatcaggt ttacccatca gcctaactcc gggtgattgg atttaacgaa
    13441 ccaggatcac aatggcgccg atggaaaaca gccaggtctt tatgtgaggt agtatatatc
    13501 cgcgaaattg tcgtgtcaag ttgaacatat ggaggggtgt gagggggtca gcacatcgta
    13561 tacaggtctt ggagcgtatg agaacaggtt tccagaatgg gttggataaa gcgtaaccgc
    13621 ttggaggtca aataccatga gggccatccc tctcggagta actaccctcg cagcatggag
    13681 ggactgtgta tctggcattg tcgcgtcttt cgataaagcg gcgtatcaca gctgccggca
    13741 accccgctga acggtcgatt tttaggcttt gtggacacgc agaaggtgct ctgggggaaa
    13801 ggcgccgcta actgagcgta tttttatgag ggttgactga acggaggaac ggacgtacca
    13861 gttaccgggt tgtggagaac cttcggctct ctatccgcga gcatccttgc ggtaatgtca
    13921 gcacgatgtt ggaacaggtt ttatttcgcg atttttcgca atttcttgat gcctttcagg
    13981 aaatgtccgt tcagcacatc taccaatccc tccacctgca agatagacat gctgtcactg
    14041 cccatcatgg tgagaaagcg cagcggcata tcattgacga tcttctccgc catcatcttc
    14101 aaataagggt ctttcgcgcc tatcttggtc acctgctggt tgatcagatg ccttatcaac
    14161 ctgccaaacc atttatcctg gatatcagtg atggtggagt tgaccgtgtg aggagcacct
    14221 ttcacccgtt cgcgagcggg tatgctcctt ccgaggatgg tggaaaaatc ggcgtcactt
    14281 accgtccacc tcgctgaggg gtggtaatat cctggcgctt gagcgcggag gtctggcagg
    14341 gctgccttaa cccgcgttgt gcaggtgagg acagccttta actgaatgtc gcggctggat
    14401 gtgccgatgc ggacctcata ctctccctct tcaacgtgcc agtctgccag gttgacattg
    14461 taataagcga aatctcgttg aaagagggtg aactcaacct gctggctttc cccgggagat
    14521 aggcgcgcct tgataaaacc tttaagttcc tgctcggcac ggatgatgac ggatcctttt
    14581 ttcgcgatgt acagctgaac cacctcgctg cctgcgcggg aaccagtgtt gctcacccgg
    14641 caggtaacct ttaatgtatc tgaatcgccc atcttatttg atgaaagctg caaatcactt
    14701 aacgagaagg tggtgtagga tagaccaaaa ccaaagggaa aacgcacagg cacatgggcg
    14761 ctgtcgtaat aacggtaacc gacaaaaata ccctcacgat attccacggt aagcgggtaa
    14821 ccggggaaat aattgaaaga agggttatcc tccagttgga acggccacgt ttcggcgagt
    14881 tttcccgaag gattgacacg acccaataac aggtcagcca ccgcgccgtt caccgtttca
    14941 cctcccaggt gcatcagcag gatggattta accctgtcag cccagggcat ctcaaccacg
    15001 ccaccgcagt agagaacaac gaccacgttg tggttcacct cgctcacagc ttcgatcagg
    15061 cgttcgtggc tctgcggcag ggcaagtgtt tcacggtcga atgtctcgga ttcaaagcgg
    15121 tcaggcagtc cgacgaacag gagaaccaca tccttaccgg cagccacctg gcaggcttca
    15181 gcgagaagcc cctcatcggg cgcatcagca tcttcaaagt aacccgcagc gtactcaaat
    15241 cgcagtccga gcgatttcaa ttcttcaaca gcgtcattta cccgtatggg ggtgatgcgc
    15301 gaactaccgc ttccctggta gcggggattt ttggcgaaag cgcctatcag cgcgacggaa
    15361 gaggaagggt ttaatggcag cagattgcct tcattcttta gcagcacagc tgattcagct
    15421 gcggcgcgcc tggcaagcgc ccggtgggcg ctcacgttat attgagcacg tttctgccct
    15481 cgcgcgcgca gcagaagcgc ggtgacattt tcggctgtct gattcagtaa tgcaacaggc
    15541 agatcgccgg ctatgaccgc ctgggcgacc ctttcgtcgt gcgtacgatc gaggtgtggc
    15601 atctccaggt cgagccctgc cgccacccct tttaccctgt cgaccaccgc tccccagtct
    15661 gaaatgacca gcccttcata cccccattcc tggcgcagaa tctgggtgag caagtattca
    15721 ttttggctga cgaattcgcc aaacaagcgg ttgtaactgc acatcacggt ccagggtctg
    15781 gcctgtttga ttgcgaactc gaacccagac aggtagattt ctcgcaaggt gcgttgatcc
    15841 atcacagcgt ccacagttaa acgtcttttt tcctggttgt tggccgcgaa atgcttaaga
    15901 gaggttccca ctcctttgct ttgcacgcct tcaataaagg cagcggccag gtggcctgaa
    15961 agataagggt cctcgctgta atattcaaaa ttacggccgc acaggggcga acgcttgata
    16021 ttcattcccg ggcccagcag tacagaaaca ccttcctgca ggcattcttc agcgagtgac
    16081 tggccaactt ctcgcagcaa cgccgggtcg aacgaacaag ccatcgatac ggcggttggg
    16141 aagcacactg ccggtacatt ctcgctgatc cccaggtctt cactgctcga aagctgctta
    16201 cgcaccccgt gcggaccatc tgtaaccatg atggaaggca accccaaccg gtcaattggt
    16261 ttcaggtacc agtaatcttt gcctgagcaa agagaggatt tctcctccag gatcatttgt
    16321 gccacaatct cgcgggcttt ggctcgtaag ttcatggttt tcactccatg ccgacgtaat
    16381 taaattcgct catttgcaat ttctccgctt ctgttcatat acaaccaaac gcaaaggtct
    16441 cgaacatcct ctcttatcct tgagcaaaca gccctgcttg aaaagagaaa agggtgcact
    16501 tttactcggc agcgggctgc ttcttaccct ggtaggcgga ccttttactg cttacttgtt
    16561 cagcaacctg gctcgatcaa agctttgttg gaaaaaatta aagagaattt ccacgccgta
    16621 aaccagccat caggttgctt gaaaagtacc atcggcgata cgagagccgc aagaaaccca
    16681 aacgccattc tcattttaca ctcgtttttt tctgacagcc ggcggcaccc tcctgccgct
    16741 acctgtgccc aggttcaggt gcgtgttcga cgagctggtt caatttctca ccggggtttg
    16801 tcaccttcgg ggattttgaa aatcgccccg ttggtataat taattcacct gctgagagat
    16861 tcattccccc caagcgcggt tactgtcagc ccatgaccac caataagtac atcaaagcgg
    16921 tttcctataa catcgtatat tttctggtaa gctcgttggc ttacctggta ctgacgccca
    16981 tcgccatccg tgtgatgggc ggtgagtttt acggtctgtg gatga
//
LOCUS       DAOSPM010000039        16975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_3398_length_16975_cov_12.741903, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000039 DAOSPM010000000
VERSION     DAOSPM010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 16975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16975
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3398_length_16975_cov_12.741903"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..423
                     /gene="rpsT"
                     /locus_tag="PLW25_04910"
     CDS             160..423
                     /gene="rpsT"
                     /locus_tag="PLW25_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOS53864.1"
                     /translation="MANIKSAIKRNKQNEKRRLRNRVYRGSARTFVARARVALARQDL
                     ETAESATMAAVSALDKAAQKGVIHKNNAARRKSRLMKRLAALK"
     gene            540..2303
                     /gene="argS"
                     /locus_tag="PLW25_04915"
     CDS             540..2303
                     /gene="argS"
                     /locus_tag="PLW25_04915"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOS53865.1"
                     /translation="MFELEQKQVEEKIHAYCRQQGLPVIEIKWTWIPFNGQWGISTSF
                     FQLAAASFGEKKGNVAARASEIAENLAVSLAKLPAFEKIEAVKGYLNLYFSTSAYFQQ
                     VLNSVLEEQDKFGHGERLHQRVMVEFSHPNTHKAFHVGHLRSAFLGDVLARILDAAGY
                     EVIRANYPGDIGLHVIKWLWNYLKFHKGEKPDADITRWMGSIYAEAVRRLEQDPALED
                     EIREMYNRWDRRDPEVVACWKETRQWSLDGFNAMYNLLGIHFDRYYFNSMFEVPGKEM
                     VEELLQKGIADDERPENGAVVVKIDEKLGLKEEKYRVMVVLRSDGTALYATEDLALAR
                     QKFLDYPDLQKSFYVVDVRQSLHFQQVFKTLELAGYEWATRCQHLPYELVNLPGNVVM
                     ASREGTVVLLEDLIREATDRALTVVQEKNRELSAEKQLEIARAVGIGAIRFPMLARES
                     NKVVTFDWESALDFNGQAAPYIQYAYVRANSIMRKIGDRIPPDLAPTYELTQPEITLV
                     DLISRMPDEIQKAARDLRPLIITNQAYELARAFNDFYTQCPVLSAEEPARSVRLRLVA
                     ASRQAIANSLAILGITAPQAM"
     gene            2336..3658
                     /locus_tag="PLW25_04920"
     CDS             2336..3658
                     /locus_tag="PLW25_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOS53866.1"
                     /translation="MTPIKTLIMGAAGRDFHNFNVFYRDNKDYEVVAFTATQIPNIEG
                     RKYPAELAGSLYPAGIPIYPESELVDLIKKFKVDQVVFAYSDVPHEVVMHKASTVLAA
                     GADFRLMGVNSTQVKSVKPVVSVCAVRTGSGKSQTTRRVSTILRDLGYKVAAIRHPMP
                     YGDLVKQKVQRFATYADLDKHECTIEEREEYEPHIDNGVIVYAGVDYEAILREAEKEV
                     DIILWDGGNNDFSFYQSDLSIVVADPHRAGHEYKYHPGETNVRLADVFVINKVDTAEP
                     GDVIAVRNTLHELNPGAIIIEGASPLFVDDPDAIRGKRVLVVEDGPTLTHGEMAYGAG
                     WVAAKRFGAGEIVDPRSCAVGTIHETFVKYPKTGNILPAMGYGDKMIKDLEDTINKID
                     VDLVISATPIDLNRIIKVNKPMQRVRYELQEIGKPDLEDILKGKFSKK"
     gene            3717..4976
                     /gene="obgE"
                     /locus_tag="PLW25_04925"
     CDS             3717..4976
                     /gene="obgE"
                     /locus_tag="PLW25_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOS53867.1"
                     /translation="MFIDEAKILCRSGRGGAGVVHFRREKYVPRGGPDGGDGGRGGDV
                     IFRVDEKLNTLLRFRYQRKFVAEDGGNGRGSNMSGKGAPNLVIPVPPGTTIYDHATNA
                     LLGDLVEPGQELMVCKGGRGGRGNQHFATSRNQAPRTAEKGEPPEEREIRLELKLLAD
                     VGIVGVPNAGKSSFLAAVTNAKPKIADYPFTTLEPNLGVASLDSETDLVLADIPGLIE
                     GAHQGVGLGDAFLKHIQRTKVLIHILDGSSTDPLADFSQINQELALFDAELAEKPQVV
                     VVNKADLPGVKESAVKVIKQLEKMGYPTFLISALTRENLTPALWKAHEVLLKVPQKEI
                     KLELPVYKPGEDPRDFSITRENTGWRVSGAAIERAAEMTYWEHFGSVRRFQRLMVALG
                     VDRALREQGIKNGDTVYILDYELEWQD"
     gene            4978..5586
                     /gene="nadD"
                     /locus_tag="PLW25_04930"
     CDS             4978..5586
                     /gene="nadD"
                     /locus_tag="PLW25_04930"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOS53868.1"
                     /translation="MTAYQRIGIFGGTFDPPHVGHLILAEECRTQLSLDLLLWVVTDN
                     PPHKRYTNVSPIQDRFMLVEKAIQGNPSFRTSRVDIDRPGPHFAIDTVNILKEEYPGA
                     EMYYLMGGDSLHDLPTWNKPQQFIDLCDGIGVMRRHADQVDLSALEKVLHGISEKVKI
                     VEAPILEISSKQIRQRIAEGMGYRYYLRDAVYQAVCELGLYR"
     gene            complement(5652..6503)
                     /locus_tag="PLW25_04935"
     CDS             complement(5652..6503)
                     /locus_tag="PLW25_04935"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HOS53869.1"
                     /translation="MKTTKLLILLALALSLMACTFSVNVPEVKTGQTRTFEVSEASPP
                     ADEVSSIKIEMGAGSLNLSGGADKLVEGTISYNIDSWKPGVTRQGNAVTIKQSTSTSV
                     AIPADTYKNSWDLKLGSTPIELTLATGAYEGDLELGGLAITALSVTDGASKSIIRFNE
                     PNAANLEQFSYKTGASDVRLEGLGNARASHIEFNGGVGRYTLDFSGINDNDIAVKMDC
                     GMSDITLVIPENVRSIITINGEMNNTNFTGTWTVKDNVYQSGTTGALIEIEIDMSVGN
                     LNLIRHE"
     gene            complement(6541..7569)
                     /locus_tag="PLW25_04940"
     CDS             complement(6541..7569)
                     /locus_tag="PLW25_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53870.1"
                     /translation="MKKTCAFATVILLFLLAFVVSPVHAQEPQPGNTISGDQVIIGNN
                     FTLEKNETLEGSLLIIGGTASTAADSKINGDLVLIGGTLTIDGAVEGDIVSIGGVVNL
                     QDSAMVNGSLTMVGATLSRSPTAQISGSISEETPRFLNFDFLRDNGLKPPLHLKTNPL
                     NRILTATFQALVMAILAVLVGLLLPRNLKNTAIALVREPVVTGGVGLLAMVVAPIILI
                     LLSITIILIPVTLLAAIAFALAVVFGNIVIGYEIGNRLSAVFKDTWHPSIAAGIGTLL
                     LSLVTGFAGVIPCVGWVLGFLASIIGLGAVVISRFGSTKYATKVISAVIQPAEEDLPT
                     EPPVAPEE"
     gene            complement(7566..8108)
                     /locus_tag="PLW25_04945"
     CDS             complement(7566..8108)
                     /locus_tag="PLW25_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOS53871.1"
                     /translation="MMKHYPYEGWILDDIDLDETRRADLEAHLALCPQCTQLSRSLRI
                     ALQAIRNAPEQTAPAGFVHKFVSSFESRRRLQERKQARTLAIVFASTALIVVLVSLYV
                     FLPELSLISLAAAAVSAVVGLLEGFQQVVSFISNAYKNLSPFTLIISGLVFAGWVLLA
                     SLTLGLSVWKLAFRKSEVKQ"
     gene            complement(8086..8679)
                     /locus_tag="PLW25_04950"
     CDS             complement(8086..8679)
                     /locus_tag="PLW25_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS53872.1"
                     /translation="MKQIDPIWIERARHGDEEAFTMLVEAYQTPVFNLCYRMLGESTA
                     AEDAAQETFWRVWQKIKSYDPQRSFSTWVLSIAAHYCIDQQRKQKITIFELDEYPDFD
                     LGDPHMPNPEITVITNEEEETLHRMINQLNPPDRAAVILKYWYGCSEEEISQMLSLTV
                     SAVKSRLHRARRQLAEVVTKNQSTTLQRGSYDEALSL"
     gene            complement(8781..9416)
                     /locus_tag="PLW25_04955"
     CDS             complement(8781..9416)
                     /locus_tag="PLW25_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOS53873.1"
                     /translation="MVKRLILVAGNIGSGKTSLTERIGERLGWHTAYESVVDNPYLPD
                     FYANMREWAFHLQVYFLGHRAQQHLDMYHDPRSAIIDRSIYEDAFIFARALYSMGNIN
                     ERDYGTYRKVFDLITATLPHPSLLIYLKCPVDTLIKRIQSRGRDIESSISRDYLALLE
                     SYYDDWTSNFDLCPILTIRTDDLDFVHEPRHLNTVVKKIKEKLAGREEIVF"
     gene            complement(9610..10467)
                     /locus_tag="PLW25_04960"
     CDS             complement(9610..10467)
                     /locus_tag="PLW25_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOS53874.1"
                     /translation="MHKVKVITDTDSSLPADVAARYRIDQVPITIQFGDDTYEAGVNI
                     NDKELFEKIDALGSLPTTAAPAPAAFRSAFAKAFAEGADSIICITVGSKISRTYDSAV
                     TTAKDDFPDRLITVVDSTCLSLQQGFMALSAAEALEAGYTHQQAVDAALQLLPRSHLY
                     GSLTTLKYLAMGGRIGQFPAKMANLLDIRPIMTMIDGKLGLLEKVRTHRAAMNRLIEL
                     FEKSTSGKPIVKAGIVHVNNLTDAALLESRLRESLPMPDEILVAEFTPGLSVHGGTGL
                     IAALLVTED"
     gene            complement(10781..13375)
                     /locus_tag="PLW25_04965"
     CDS             complement(10781..13375)
                     /locus_tag="PLW25_04965"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOS53875.1"
                     /translation="MDTPPRTAQKSLLDLGGIPNNLPTVTLSDNARQVLLKRYVRRNP
                     DGSPAETVEEMFWRVASTIANIETRWSADVDALAQRYYILLSSKAFFPNSPTFTGAGT
                     PLGQLAACFVLPINDDMGRDSAGIFQTLRDAALIQQTGGGNGFSFSRLRPKGSHVKTS
                     AGQATGPVGFLRVYDHAFGEVAQGGTRRGANMAVLRVDHPDIEEFIACKTNENQITNF
                     NISVGITDAFMRAVEDDVEWNLRFPDLSDPRAKKFIGTFEQAEDAGIPFRIYKTIRAR
                     DLFQRIVKQAHHNGEPGLLFLDAANRANPVPHLYQLEATNPCGEQWLGPYENCCLGSI
                     NLAQHLGPDHSVDWIKLRENTVLATRFLDDVVDANAYVPAVPQLKEAAMRNRRIGLGI
                     MGLADLMYHVGVRYGSPAAEDFASQVMEFVRYHAMKTSIELARERGPFPSIKGSIYDP
                     ENLVWQPPVSLIETQQYWGRPTIDWSEISEGIRRYGIRNAAQTTVAPTGTIATVAGCE
                     GYGCEPVFALAYIRHVNDNGKDLKLTYTSPAFEQALLEAGVSEMDRQDIYDQVMKDGS
                     CQHIKSVPEMVKNTFVVSADISAEEHVRMQAALQAFVDNSLSKTINFPENATEEDVAT
                     AYLRAWELNCKGITVYVTGSREEVVLETNATASKKAPAADDAMPDQIKLWQESKKPRP
                     RSLIGRTFSIETPLGKAFITINENDAAQPFEVFINTAKAGSDTAAVSEAIGRLISYVL
                     RLASPIEPSQRLTDLTRQLLGIGGGRSLGFGPNRVRSLPDGVAQVLEEYLDERAIRLG
                     KADGKPGVVQQYLINDITDLTPPVEEPPASPPVVQRIGDLCPECGVAAVINEEGCRKC
                     YACGYSEC"
     gene            complement(13384..13833)
                     /gene="nrdR"
                     /locus_tag="PLW25_04970"
     CDS             complement(13384..13833)
                     /gene="nrdR"
                     /locus_tag="PLW25_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOS53876.1"
                     /translation="MLCPYCQKDQSRVIDTTHDSRGGIRRRRECEHCQQRFSTYERPI
                     LATPLIIKRSGTREQFNREKLLEGIRISCAKRPVSADDINHLVNEIETALQKMGREEV
                     SSRVVGDMVIDGLKRLDQIAYIRYAIVYLQLTDLQSIRKEIDRLLTS"
     gene            complement(14393..>14916)
                     /locus_tag="PLW25_04975"
     CDS             complement(14393..>14916)
                     /locus_tag="PLW25_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOS53877.1"
                     /translation="DFADVKNVMSEGGAALMAVGRASGEDRAIKAAEMAIFNRLLDIT
                     IDGARGILFNVTGGPNLTLHEVNQAAAIIKETAHPEVNLIFGAVINPDMGDDVRITVI
                     ATGFDRANGSPIVRKPVTKVNDESRKPAFQTNTEIKEVTPVNTPVNEFVPVKMDTRNL
                     DLPTFLRIHLDEK"
     assembly_gap    14917..15016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15017..15744)
                     /gene="ftsZ"
                     /locus_tag="PLW25_04980"
     CDS             complement(<15017..15744)
                     /gene="ftsZ"
                     /locus_tag="PLW25_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOS53878.1"
                     /translation="METNRTPQYASPRSSFEAESKSPIETFARIKVIGVGGGGCNAVN
                     RMIEQGAQGIEFITVNTDAQALLMSKAPTKIRIGDKTTNGLGAGGNPEVGRKSAEESY
                     EELTQVLKGSDMVFITAGMGGGTGTGAAPIIAKIAKDSGALTIGVVTRPFTFEGAKRL
                     QSAEQGIARLKEQAHTLIVIPNDRLLQMVDRKSTLQESFRLADEVLQQGIQGISEVIT
                     IPGLINLDFADVKNVMSEGGAALMA"
     gene            complement(15798..>16975)
                     /gene="ftsA"
                     /locus_tag="PLW25_04985"
     CDS             complement(15798..>16975)
                     /gene="ftsA"
                     /locus_tag="PLW25_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOS53879.1"
                     /translation="GDTDMRILGVGIEPALGIKKGNVVDISTATQSISRSIEKAQRTS
                     GLEIESALVSLAGSHVMAMNSRGVVGISGGVISQEDVDRALEAAMAVAMPHNREIIHV
                     IQRGFTVDGQEGIQSPVNFSGFRLEVEAHIITAAAATVENLRQCVNGCNVDVLQFVLN
                     PLASGEVVLNKPERDMGVCICDIGGGTSDLAIYINGDVWHTDVLPVAGNQITNDIAIG
                     LHISPEQAEQIKKQYGHAITGEVGEGEFFTLRTFGDDQPKQISRRELAYIIEARVEEI
                     FQLLHQEVRRSGYENLLPAGMILTGGTSQLPGIRQLAADVLNMPVRVARPENLVGLVD
                     QLQSPAYSTSVGLLKWSELMTQPSYAVTHPRKPKTSGFKPSVLNWDKLKVLVERLLP"
BASE COUNT         4020 a         4158 c         4836 g         3861 t
ORIGIN      
        1 tggaactgcc gggtgtggct gccctgttgg ataagtaaat ctctcaactc tcaacacgat
       61 tatctggaca gaaagcaggt gatgcggtag aattcttgcg ccacctgaac agtgcttacc
      121 ctcgaccctc gcactgacgg aattttttgg aggaacaagt tggcgaatat taaatctgca
      181 ataaaacgca acaagcaaaa tgaaaaacga agactgcgca accgggttta ccggggttca
      241 gcgcgcacgt ttgtcgcccg cgcccgggtg gcattggccc gccaggacct tgaaaccgcg
      301 gaaagcgcga caatggctgc tgtgagcgcc ctcgacaagg ctgcacagaa aggcgttatt
      361 cataagaaca acgcagcgcg gcgcaagagc aggctgatga agcgcctggc agcattaaaa
      421 taagatcgtt ttttcaaaac cgagcgggag gagaaacctc ccgctcttgc tgttaaaaac
      481 cagagccgtt tttttcccta tgatataatc catctgccta tcgaggaaaa cgaaaaaaca
      541 tgtttgaatt ggaacaaaag caggtcgaag agaagatcca tgcttactgc cggcagcagg
      601 ggctgccagt aatcgaaata aaatggacat ggatcccttt caacggccag tggggaatca
      661 gcacctcttt cttccagctg gctgccgctt cttttggtga aaagaagggc aacgtggcgg
      721 cacgcgccag cgaaatcgct gaaaacctgg ctgtgtctct cgcaaaattg cccgcgttcg
      781 aaaaaatcga agcggttaaa gggtatctta atctctattt ttcaacatct gcttactttc
      841 aacaagtgct taattcggtt ttggaagaac aggataagtt tggtcatgga gaacgcttac
      901 accagcgggt tatggttgag ttctctcacc caaacacaca caaagccttc cacgtggggc
      961 acctgcgcag cgctttttta ggcgatgtac tggcgcgtat tttggatgct gctggttatg
     1021 aagttatcag ggcgaattat ccgggcgata ttgggttgca tgtgatcaaa tggttgtgga
     1081 attacctgaa gttccataaa ggggaaaaac cagacgctga tattacacgc tggatgggat
     1141 cgatctacgc ggaggcggtc aggcgcctgg agcaggaccc tgcgcttgaa gacgaaataa
     1201 gggagatgta caaccgctgg gacagacgtg accctgaagt ggtggcgtgc tggaaagaga
     1261 ccaggcagtg gtcgctggat ggcttcaatg ccatgtacaa cctgttgggc attcacttcg
     1321 atcgttatta cttcaattcg atgtttgaag tgccgggcaa ggagatggtt gaagagttgc
     1381 tgcaaaaagg gatcgcggac gatgagcgcc cggaaaatgg cgctgtggtc gttaaaatag
     1441 atgaaaaact cggattgaaa gaagaaaaat accgggtgat ggtcgttctg cgatcagatg
     1501 gaacggcgct gtacgccaca gaggatctgg cgctggcgag acagaaattc ttggattatc
     1561 ctgacctgca gaagtcattt tatgtagtgg atgtacggca gtcattgcac tttcaacagg
     1621 tgttcaagac attggaactt gccgggtatg aatgggcaac tcgctgtcaa catttacctt
     1681 atgaactggt gaatttaccc gggaatgtgg tgatggcttc tcgagaaggc acggtagttc
     1741 ttctagaaga ccttatccgg gaggcaactg accgcgcctt gaccgtagtg caggagaaga
     1801 accgtgagct ctctgctgaa aaacaactgg agatcgcgcg ggcagtggga attggcgcaa
     1861 tcaggttccc aatgcttgcg cgcgagagca ataaagtggt tacctttgac tgggagtcag
     1921 cgctggactt taacgggcag gcagcccctt atattcaata cgcgtatgta cgcgcaaata
     1981 gcattatgcg taagatagga gaccgcattc cgcctgacct ggctccaacg tatgaactta
     2041 ctcagcctga gatcaccctc gttgacctga tcagccgcat gcccgatgag atacaaaaag
     2101 cagcccgaga tttgagaccc ttgatcatta caaaccaggc gtatgaactg gcgcgcgcgt
     2161 ttaacgattt ttacacgcaa tgcccggtgc tgagtgccga agaaccggcc cgctccgtac
     2221 gattgcgact tgtagcagct tctcgacagg caattgcaaa cagtctggca atactgggca
     2281 tcacagcccc acaagcgatg tgaaaataat ctaacaaaaa aaggagacca ttcaaatgac
     2341 cccaattaaa accctcatca tgggtgctgc cggtcgcgat tttcataatt tcaatgtttt
     2401 ctatcgcgac aacaaggatt atgaagtcgt cgctttcact gccacgcaaa ttcccaatat
     2461 cgaaggccgc aaatacccgg ctgagctggc gggttctctc tacccggcgg ggatccccat
     2521 ctacccggaa agtgaactgg tggacctgat taagaaattc aaagtagacc aggtggtttt
     2581 cgcgtacagt gacgtgccgc atgaagtggt gatgcacaaa gcgtctactg tgttagcggc
     2641 tggggcggac tttcgattaa tgggggtaaa cagcacccag gtcaaatcgg ttaaacccgt
     2701 ggtatctgtt tgcgcggtgc gtaccgggtc gggtaaaagc cagaccacac gccgtgtttc
     2761 cacaattctg cgcgacctgg gttacaaggt ggctgccatc cgccacccaa tgccttatgg
     2821 tgacctggtc aagcaaaagg tgcagcgatt tgccacctac gctgacctgg ataagcatga
     2881 atgcaccatt gaagagcgcg aggaatacga acctcatatt gacaatggcg tcatagtcta
     2941 cgctggcgtg gattacgaag cgattctgcg cgaagcggaa aaagaagtgg atattatttt
     3001 gtgggacggt ggaaacaacg acttctcatt ctatcagtct gatttgagca ttgtcgtggc
     3061 tgacccgcac cgagccggcc atgaatataa atatcacccg ggtgaaacga atgttcggct
     3121 ggcggacgtg tttgtgatca ataaagtcga taccgcggaa cccggtgatg ttatcgccgt
     3181 gcgcaacaca ctgcatgaat taaacccagg cgcgatcatc atcgaaggcg cttcaccgtt
     3241 atttgtggat gaccccgatg ccatccgtgg aaaacgcgtt ttggtggtgg aagacggtcc
     3301 gacgctgacc cacggcgaaa tggcgtacgg ggcaggctgg gtggctgcca agcgatttgg
     3361 cgcgggcgaa attgttgacc cacgttcttg cgcggttggc acgatccatg agacgtttgt
     3421 gaaatacccg aagactggca atatcctgcc ggcgatgggg tatggagata aaatgatcaa
     3481 agaccttgag gatacgatta acaaaatcga tgtggacctg gtcatctcag ccacacccat
     3541 cgatctcaat cgcattatca aggtgaataa accgatgcaa cgcgtgcgtt acgaactgca
     3601 ggaaattggt aaaccagacc tggaagacat tttaaaaggg aaattctcca agaagtagca
     3661 gaaaagcaaa acaggctcgg cgcgaaaaag ccgagcctgt tgcgggatga aacaggatgt
     3721 tcatcgacga agccaaaata ctctgcaggt caggccgcgg cggggcggga gtggtgcatt
     3781 tccgccgcga gaagtacgtt cctcgtggag ggcctgatgg cggagacggc ggcaggggcg
     3841 gggatgttat ctttcgcgta gatgaaaagc tgaacacatt gctccggttc cgttatcaac
     3901 gcaagtttgt ggcagaggat ggcggaaacg gtcgcggtag caacatgagc ggtaaagggg
     3961 cacctaacct ggtgattcct gttccccccg ggacgacaat ttatgaccat gcgacaaacg
     4021 cgcttctcgg tgacctggtt gagccagggc aggaactgat ggtgtgcaag ggcggaagag
     4081 gtggcagggg aaaccagcat tttgccacct cgcgcaatca ggcgccacgc accgctgaaa
     4141 aaggagaacc tcctgaagaa cgggaaatca ggctggagct gaaactgctc gctgacgtgg
     4201 gaatagtggg cgtcccgaac gctggtaaat ccagtttttt agcagcggtt accaatgcca
     4261 aacccaaaat tgcggattat ccttttacca cccttgagcc caacctgggc gtagccagcc
     4321 tggacagcga aactgacctc gtcctggcgg atatccctgg gctcatcgaa ggtgcgcatc
     4381 agggggtagg gctgggagac gccttcctta agcacattca acgtaccaag gttctcattc
     4441 acatactgga tggcagttcc actgacccac tggcagattt cagccagatc aaccaggaac
     4501 tggcgctgtt tgatgctgaa ttagcagaga aaccgcaggt agtggtggtg aacaaagccg
     4561 acctgccggg ggtgaaagaa agtgcggtta aagtaatcaa acaactggag aagatgggat
     4621 atcccacttt tctcatctca gcgctgacgc gagagaactt aacccctgca ctctggaaag
     4681 cgcacgaagt ccttttgaaa gtacctcaaa aagaaatcaa gcttgaactg ccagtgtata
     4741 aaccaggtga agacccgcgt gatttttcca tcacacgcga aaatactggt tggcgggtga
     4801 gcggggcggc gatcgaacgc gcggcggaaa tgacctactg ggagcatttc ggttctgtgc
     4861 ggcgcttcca acgcctgatg gttgcgttag gtgtcgatag agcgctgcgc gaacagggaa
     4921 ttaagaacgg cgataccgtg tatatcctcg attacgaact ggaatggcag gattgatatg
     4981 acagcctatc aacgtattgg aatttttggc ggaacgttcg accctccaca tgtagggcac
     5041 ctgatcctgg cggaggaatg ccgcacgcag ctttctttag acctgctctt gtgggtggta
     5101 acagacaacc cccctcataa acggtatacc aatgtcagcc ccattcaaga tcgttttatg
     5161 ttggtagaaa aagccattca agggaaccca tccttcagaa cttcgcgggt agatattgac
     5221 cgcccgggcc cccattttgc cattgatacg gtcaacatcc ttaaagaaga gtacccagga
     5281 gcggaaatgt attatctgat gggtggtgac tcgcttcacg atttgccaac ctggaataaa
     5341 ccgcagcaat ttatcgattt atgtgatggt ataggtgtga tgcgccgcca cgccgaccag
     5401 gtggacctat ctgcactcga gaaggtattg cacgggatat cagagaaggt gaaaatcgtt
     5461 gaagcaccga ttctcgagat ttcttctaaa cagattcgac agcgaatcgc tgaaggcatg
     5521 ggctaccgct attacctgcg tgacgcggtg taccaggcgg tatgcgagct gggtttgtac
     5581 cgttaggcaa aaatgatttg atgataaaaa aattccggcg tcaacccgcc ggaattttaa
     5641 ggttcatccg cctactcgtg gcggatcagg ttaaggttcc cgactgacat atcgatctct
     5701 atttcaatga gcgctcctgt cgtacctgat tggtagacat tgtccttaac cgtccaggtg
     5761 ccagtaaaat tggtgttatt catttcgcca ttgatggtga tgatcgagcg cacattctca
     5821 ggtattacga gcgtgatatc gctcataccg cagtccattt tcacggcaat atcgttgtcg
     5881 tttatccccg agaaatccag ggtgtatctc cccacccctc cattgaactc aatatggctg
     5941 gcgcgggcgt tacccaaacc ctccaggcga acatcagacg ccccggtttt ataggagaac
     6001 tgctcgaggt ttgcggcgtt gggctcatta aacctgatga tggacttgct ggcgccgtca
     6061 gttaccgaaa gagcagtgat ggcaaggcca ccaagctcca gatcgccttc gtaggcgcca
     6121 gtggcgagag tgagctcaat aggcgtggaa ccaagtttga gatcccagga gttcttgtat
     6181 gtatcagcag ggattgcaac actggtagag gtagactgtt tgatcgtaac agcattaccc
     6241 tggcgggtga cgcccggctt ccaggagtca atattgtaag aaatggtgcc ttcgactaac
     6301 ttatcggcac ccccactgag gtttaacgac cctgctccca tttcgatttt aatactggac
     6361 acctcatcag caggtgggga ggcttcactc acttcaaatg ttcttgtctg tccggttttc
     6421 acctcaggta cgtttaccga aaaggtgcag gccatcaaag agagggcgag cgcgagtaag
     6481 atgagcaatt tggttgtttt catttttcaa tcaccttcat ttcgatagaa taaaacgttt
     6541 tcattcctcg ggggcaacag gcggttctgt ggggagatct tcttccgctg gctggatcac
     6601 ggctgagatt acttttgtgg cgtatttggt cgaaccgaaa cggctgatga ccaccgcgcc
     6661 gagacctatg atggaagcga ggaaccccag cacccagccc acgcagggaa ttaccccggc
     6721 gaacccagtg accaggctga gcagcagcgt gccaataccg gctgcaatag aaggatgcca
     6781 ggtgtcttta aacaccgcag aaaggcggtt gccaatctcg tatccgatga cgatattgcc
     6841 aaagaccact gccagtgcga aggcaatggc tgccaataac gtcacaggaa taaggataat
     6901 agtgatgctg agcaatatta aaatgatagg agccaccacc atagcgagca ggccaacacc
     6961 tccagttaca accggctccc gtaccaacgc gatggctgtg ttcttgagat tgcgcggcag
     7021 cagcaggcct accagtaccg ccagtattgc catcaccaac gcctgaaagg tagcggtgag
     7081 tatcctgttc aatgggtttg ttttaagatg gagcggtggt ttcaaaccgt tatccctcag
     7141 gaaatcaaaa tttaagaacc gcggtgtttc ttctgagata ctgccgctta tttgcgcggt
     7201 tggggaacgt gagagggtag cgccgaccat tgtgaggctg ccattgacca tcgcgctgtc
     7261 ttgcaggttt accacaccgc ctattgaaac aatatcccct tctactgcgc catcgatggt
     7321 caacgttcca ccgataagaa cgaggtcacc gttgatttta gagtcagcgg cggtggaagc
     7381 cgtgccgccg atgatgagca gactgccctc gagcgtttca ttcttctcga gtgtgaagtt
     7441 attgccaata atcacctggt cgcctgagat agtgttaccc ggttgaggtt cctgggcgtg
     7501 aacaggcgaa acgacgaagg cgagaaggaa gagcaggatg accgttgcga aggcgcaggt
     7561 ttttttcatt gttttacctc tgattttcta aacgcgagct tccacacaga aagcccaagc
     7621 gtgagactgg ccaggagaac ccagccggcg aaaaccaggc cgctgataat aagcgtgaaa
     7681 gggctgagat tcttgtaagc gtttgaaatg aaggaaacca cctgttgaaa accttcaagc
     7741 aggccaacga ctgcggaaac ggctgcggca gccagtgaaa taagcgaaag ctcggggagg
     7801 aacacataaa gagaaactag cacgactatg agagcggtag aggcaaacac aatggcgagg
     7861 gtgcgcgcct gcttgcgctc ctgcaggcgg cgccggcttt cgaacgaaga aacgaacttg
     7921 tgaacgaacc cggcaggggc cgtctgttca ggtgcgttgc ggatggcctg cagcgcgatg
     7981 cgtagtgagc gtgaaagctg ggtgcactgc gggcaaagag ccaggtgcgc ttccaggtcg
     8041 gcgcgccgtg tttcatcaag gtctatgtca tccagtatcc aaccttcata gggataatgc
     8101 ttcatcatat gatcctcttt gcagcgttgt tgattgattt ttggtcacga cctcagcgag
     8161 ctgtcgcctg gcgcgatgca ggcggctctt aaccgcgctg actgtcaatg aaagcatttg
     8221 actgatttct tcttccgagc atccatacca atattttaaa atcaccgcgg cgcgatcagg
     8281 cgggtttagc tggttgatca tacggtgcag tgtttcctct tcttcattgg tgatgaccgt
     8341 gatctcgggg ttgggcatat gcgggtcgcc aaggtcaaaa tcgggatact catccagttc
     8401 aaaaatcgtg atcttctgct tgcgctgctg gtcaatgcag taatgcgccg cgatggataa
     8461 cacccaggtg ctgaaagagc gctgcgggtc gtagcttttt attttctgcc aaacgcgcca
     8521 gaacgtttcc tgggcagcgt cttcagcagc tgtgctctcg ccgagcatgc gatagcacag
     8581 gttaaaaaca ggggtttggt aggcttcaac caacatggtg aacgcttctt catctccatg
     8641 gcgggctctc tcgatccaaa ttgggtcaat ctgcttcatt gccatcccgc tttattttat
     8701 tgtctagcaa atatacgcag aggggtgaaa taggttgcag ccacattttt actactttac
     8761 tgctgattga gcaggaagta ttaaaagacg atttcttccc ggccagcaag cttttctttg
     8821 atctttttca ccacagtatt taggtggcgc ggctcgtgaa cgaaatccag gtcgtctgtg
     8881 cggatggtga ggatcgggca gaggtcgaaa ttgctcgtcc agtcatcgta ataagattca
     8941 agaagcgcca ggtaatcacg ggaaatggaa gattcaatat cgcgcccgcg cgattggatg
     9001 cgttttatca gggtatccac agggcatttg aggtatatca acagcgaggg atgaggtaag
     9061 gtggcggtga tgaggtcaaa gaccttgcgg taggtgccgt aatcgcgttc gttgatattc
     9121 cccatggaat agagcgcgcg ggcgaagatg aaagcatctt catagatgct gcggtcaatg
     9181 atggcggaac gcgggtcatg atacatatcc agatgttgtt gagcgcggtg ccctagaaaa
     9241 tagacctgca agtgaaaagc ccattccctc atattggcgt agaaatccgg caggtatggg
     9301 ttgtcgacga cagattcgta agcagtgtgc cagccgaggc gttcaccgat gcgctcggtg
     9361 agcgaggtct taccggaacc gatgttaccc gcgacgagga tgagtctctt gaccatgcgc
     9421 gtgctcctgt aaccaagaat acatctatta tacggcaatg cgaggaaggc accgttacag
     9481 gcagtatttc agccggaagg agagggtgaa aaacagccgc gttgggattt tattcgcctg
     9541 ataaggtgat ggatggcatc acaaaccgcc gggcttgccg gcggtaattt tctgtgaaag
     9601 gggaaacgct caatcttcgg taacaagaag ggcggcgata agaccagtac cgccgtgaac
     9661 tgaaagcccg ggggtgaatt cggcgacgag aatctcgtca ggcataggca ggctttctcg
     9721 cagtcgcgat tcgaggaggg cggcatccgt aaggttgttg acgtgcacga taccggcttt
     9781 aacgatgggt ttgccagagg tggacttttc gaagagttca atcaggcggt tcatagcggc
     9841 gcggtgggtg cgcacttttt cgagcaggcc aagcttgccg tcgatcatgg tcatgatggg
     9901 gcgaatatcg agcaaattgg ccattttcgc cggaaactga ccgatgcgac cgcccattgc
     9961 caaatacttt aatgttgtca gcgagccgta aaggtgggaa cgcggtaaca gctgaagggc
    10021 ggcatcaacc gcctgctggt gcgtgtagcc ggcttccagg gcttcagcgg cggagagcgc
    10081 catgaaaccc tgttgtaatg aaaggcaggt ggaatcaacc acggtgataa gtctatcggg
    10141 gaagtcgtct ttggcagtgg tgacggcgga atcgtaggtg cggctgatct tgctgcccac
    10201 ggtgatgcag ataatggaat cggcgccctc cgcaaaggct ttggcgaagg ctgatctaaa
    10261 ggcagcggga gcaggggcgg cggtagtggg caggctgcca agcgcgtcaa ttttttcaaa
    10321 aagctctttg tcgttgatgt tgacaccggc ttcataggtg tcatcgccaa actgaatggt
    10381 gatgggcacc tggtcaatac gatagcgcgc agcgacgtcg gccggcaggc tggaatcggt
    10441 atcggtgatg actttgactt tatgcatgca tactcctaaa ccacgaccaa attttcttca
    10501 gcagaatgag cattcacctt tatgcattaa ccatctattt gcgttttcga ttcgctgaaa
    10561 accactcaac cagaactgca tggtcaaccc ttaaaaacgc aggcggttgg acgatcaaac
    10621 ccttgctgaa taaatcgcat agaccataag ccgcatcatc caatttccca acgagcgata
    10681 agggagtgat ttttctttgc cggtcatttg atccttttaa atccatcggg gcgtttgaaa
    10741 aaatgccccg atcctttgtt tcaccgtttg ctgcaggttc tcagcactcg ctatagccgc
    10801 aggcgtagca cttgcggcag ccttcttcgt tgatgacggc ggcgacgccg cattcggggc
    10861 aaaggtcgcc aatgcgctgc acaacgggcg ggctggcggg cggctcttca acgggtgggg
    10921 tgaggtcagt gatatcgtta atgaggtatt gctgcacaac gccgggcttt ccatcggctt
    10981 tacccaggcg gatagcgcgc tcatcaaggt attcctcgag cacctgggcg acgccgtcgg
    11041 gcagggagcg gacgcggttc gggccaaaac caagcgaacg cccgccgccg atgcccagca
    11101 gctggcgggt gaggtcggtg aggcgctgag agggctcaat cggcgaagcc aggcgcagca
    11161 cgtaagagat gaggcggccg atggcctcgc tgacggcggc ggtatcagag ccggctttgg
    11221 cggtgttgat gaagacctcg aaaggctggg cggcgtcgtt ctcgttaatg gtaatgaaag
    11281 ccttgcccag cggggtttca atggagaagg tgcgtccgat cagagaacgc ggtctgggct
    11341 ttttggattc ttgccacagt ttaatctggt ccggcatggc gtcatcagcg gcaggggctt
    11401 ttttactggc ggtggcgttg gtttcgagca ccacctcttc gcgcgaacca gtgacgtaaa
    11461 cggtgatgcc cttgcagtta agttcccagg cgcgcaggta ggcggtggcg acgtcttcct
    11521 cggtggcgtt ctcagggaag ttgatggtct tggagagcga attgtccaca aaggcttgca
    11581 gcgccgcctg catgcgcacg tgttcctcgg cgctgatatc agcgctgacg acaaaggtgt
    11641 tcttgaccat ctcagggact gacttgatat gctggcagga gccatctttc atcacctggt
    11701 cgtagatatc ctgacggtcc atctcgctga cacccgcttc aagcaaggcc tgttcgaagg
    11761 cgggcgaggt gtaagtgagt ttaaggtctt tgccgttgtc attaacgtgg cggatgtacg
    11821 ccagggcgaa aaccggctcg cagccgtatc cttcacaacc ggcgacagtg gcgatggtgc
    11881 cggtgggggc cacggtggtt tgcgcggcat tgcggattcc gtaacgccga ataccctcgc
    11941 tgatctcgct ccaatcaatg gtggggcgcc cccagtactg ctgggtttcg atgagcgaca
    12001 cgggcggctg ccagacaagg ttttcggggt cgtagatgct gcccttgatg gaggggaagg
    12061 ggccgcgctc acgcgccagt tcgatgctgg ttttcatggc gtggtagcgc acaaattcca
    12121 tcacctgaga ggcgaaatcc tcggcggcgg gcgaaccgta acgcactccc acgtgataca
    12181 tcaggtcagc caggcccatg atgcccagcc cgatacggcg gttgcgcatg gcggcctctt
    12241 tgagctgtgg tacagccggc acgtaggcgt tggcatcaac gacgtcatct aagaagcgag
    12301 tggccagcac agtattctcg cgtagcttga tccaatctac gctgtggtcg ggcccaaggt
    12361 gctgggcgag attgatggag cccaggcagc agttttcgta tggaccgagc cattgctctc
    12421 cgcaggggtt agtggcttcc agttggtaaa ggtgggggac ggggttggcg cggttggcgg
    12481 cgtctaaaaa gagcagcccg ggctcgccgt tatgatgtgc ctgcttgaca atgcgctgaa
    12541 agaggtcgcg cgcgcgaatg gtcttataaa tccggaaggg gatgcccgca tcttccgcct
    12601 gttcaaaggt gccgatgaac tttttcgcgc gcgggtctga aaggtcagga aagcgcaggt
    12661 tccattccac atcgtcttcc acggcgcgca tgaaggcgtc ggtaatgcca acggaaatat
    12721 taaagttggt aatctggttc tcgttggtct tacaagcaat aaactcttcg atatccggat
    12781 ggtccacgcg caacacggcc atgttggcgc cgcggcgggt gccgccctgg gcaacttcgc
    12841 caaaggcgtg gtcataaacg cgtaaaaaac ccaccgggcc ggtagcctga ccggctgaag
    12901 ttttcacatg agagccctta gggcgcaggc gcgaaaaaga aaagccattg ccgccgccgg
    12961 tttgctggat gagggcggca tcgcgcaggg tctggaagat accggcggag tcgcgaccca
    13021 tatcatcatt gatgggaagg acgaagcagg cggcaagctg ccccaggggg gtgccagcac
    13081 cggtaaaagt gggagaattc gggaagaaag ccttggaact gagcaggatg tagtatcgct
    13141 gcgcgagggc gtcaacatcg gcggaccagc gggtttcaat gttcgcgatg gtggaggcta
    13201 cacgccagaa catctcttcg acggtttcag ctggggagcc atcgggattg cggcgcacgt
    13261 aacgtttgag cagcacctgg cgggcgttgt ctgagagtgt tacggtgggc aggttatttg
    13321 gaatcccgcc caggtcgagg agtgattttt gggcagtgcg cggtggtgtg tccatgtagt
    13381 ctcctaggag gtcagtaatc ggtcaatttc tttacggata gactgcaggt cagtaagctg
    13441 cagatacacg atggcgtatc gtatataagc gatctggtcc aaccgcttga gcccgtcgat
    13501 aaccatatct ccaaccacgc gtgaggagac ctcttcacgt cccatttttt gcagggcggt
    13561 ttcaatctcg ttcaccaggt ggttgatatc atccgcagat acggggcgct tggcgcagga
    13621 gatacggatg ccttcaagga gtttttcacg gttgaactgc tcacgggtgc cagaccgctt
    13681 gatgatgagc ggggtggcga ggatggggcg ttcataagtg gagaagcgct gctgacaatg
    13741 ttcacattca cggcgcctgc gtattccgcc gcggctgtcg tgtgtggtgt caattaccct
    13801 tgattggtct ttctggcaat atggacaaag catcctaccc tccaaattga ccccatgaac
    13861 attgtgccgg tacctctgac cggtggctgg gttgtgatgg tcgcctggaa atgacatccc
    13921 ccatatatag ggggagctac ataatcagac cgtacatcta ggaagtattc taacatacag
    13981 cgtatttcga ttcaagccaa aaaagggtca attttcttaa aactttcaga aatgataata
    14041 aaactgattc tgagaagtgt catggttgat gaagaaaaaa ccgagggaaa tacggtaaca
    14101 aacccaattt gatccaatag ttatgttaaa aactggcaaa aatcttcaaa ttactaaact
    14161 cctccttaaa gtgagataac cttaaaaaaa gcaggtagat aaaaagagag aggagcagcc
    14221 aggcgcgggc aatttgttcc gattaaatga ttcaccggtg cagcgattaa atcgcgcagc
    14281 tggcggggac cagccgccag ccgcgaatga atgatatgac cgggcagagc gctgtgcgcg
    14341 cccggcatat cttatggagg acatggttca agcatgcgaa gcaagcgtga gcctacttct
    14401 catccagatg aatgcgcaag aaagtgggta aatccaaatt cctggtatcc atcttgacag
    14461 gaacgaactc gttcaccggc gtattgactg gcgtgacctc tttgatttct gtgttggtct
    14521 ggaaggcggg cttcctgctt tcatcattga cctttgtgac cggtttcctc actatgggcg
    14581 aaccatttgc gcggtcaaac ccggtggcga tcacggtgat gcgcacatcg tcgcccatat
    14641 ccgggttgat aaccgcgccg aagatgaggt taacctcggg atgggcggtt tctttaataa
    14701 ttgctgccgc ctggttgacc tcatggagag tgaggttggg accgccggtg acattaaaga
    14761 ggataccgcg ggcgccatca atggtgatgt cgagcagacg gttgaaaatg gccatttcag
    14821 ctgcttttat cgcgcggtct tcaccgctgg cgcgccccac cgccatcaag gcagcgccgc
    14881 cttctgacat cacgttctta acgtcggcga agtcaannnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnngcca tcaaggcagc gccgccttct gacatcacgt tcttaacgtc
    15061 ggcgaagtca agattaataa gaccggggat cgtaatcact tcggagatgc cctgtatgcc
    15121 ctgttgcaat acttcgtcag ccagcctgaa ggactcctgc agagtggatt tgcggtccac
    15181 catttgcagc aggcgatcgt taggaatgac gatgagggtg tgtgcctgtt cttttaggcg
    15241 ggcaatgccc tgctcagccg attgcaagcg cttggcgcct tcgaaggtga aggggcgggt
    15301 gaccacgccg atggtgagcg cgccactatc tttggctatc tttgcaataa tgggcgctgc
    15361 gccggtgccg gtaccgccgc ccatgccagc ggtgataaag accatatcgc tgcctttgag
    15421 cacctgggtc agctcctcgt aagattcctc ggcggatttg cgtccaacct cgggattccc
    15481 gccggcgccc agaccattgg tggtcttatc accgatgcga attttggtgg gtgcctttga
    15541 catcaacagc gcctgggcat cggtgttcac tgtgatgaac tcgattccct gggctccctg
    15601 ttctatcatg cggttgacgg cattgcatcc tcctccgccg acgccaatga ctttgatgcg
    15661 ggcgaaggtt tcgatgggag atttactttc tgcttcaaaa ctgcttcttg gtgatgcgta
    15721 ttgtggtgtt ctgttggtct ccattgtttc atcctccatt ttgattttat tctgtagcgg
    15781 tcaacaatga ccgtaactca cggaagcaat ctttccacca gcactttcaa tttatcccaa
    15841 ttcaggacag atggcttgaa gccgctggtt ttaggcttac gcgggtgggt aaccgcgtaa
    15901 gagggctggg tcatcagctc cgaccatttc agcagaccaa cgctggtgga ataagctggt
    15961 gattgcagct ggtcaaccaa cccgacgagg ttttcggggc gggcgacgcg caccggcatg
    16021 ttcagcacgt cggcagcaag ctggcggata ccaggaagct gactggttcc accggtgagg
    16081 atcatgcccg ctggaaggag attttcgtac ccggaacggc gcacttcctg atgcagcagt
    16141 tgaaagatct cttcgacgcg ggcttcgatg atgtaagcca gttcgcggcg gctgatctgc
    16201 ttgggctggt catcgccaaa ggttctaagg gtaaagaact cgccttcgcc gacttcgcca
    16261 gtgatggcgt gcccatactg ctttttgatc tgttctgcct gctccgggct gatgtgcaaa
    16321 ccaatggcaa tatcatttgt gatttggttg cctgccaccg gcagcacgtc ggtgtgccac
    16381 acatctccat tgatataaat ggcaaggtcg ctggtgccgc caccgatatc gcaaatacaa
    16441 acgcccatgt cgcgctcggg tttattgagc accacctcgc ccgaagccag ggggttgagc
    16501 acgaattgca acacatccac attgcacccg ttgacgcact gccgcaagtt ctcaacagtg
    16561 gctgctgcgg cggtgatgat gtgagcctcg acctccagcc ggaagccaga gaagttgact
    16621 ggtgattgaa tgccttcctg tccatccacg gtgaaaccgc gttggatcac gtgaatgatc
    16681 tcacggttat ggggcatggc gaccgccatg gcagcctcaa gggctcggtc cacatcttcc
    16741 tgagagataa ccccgccgct gatgcccacc acaccgcgtg agttcatggc cataacgtgc
    16801 gaaccagcca ggctgaccag ggcagattcg atttcaagcc cagatgtgcg ttgggctttt
    16861 tcgatagagc gagagatgga ttgggtagct gtgctgatat ccaccacgtt gccttttttg
    16921 atgcccaggg ccggctcgat gcccacaccc agaatgcgca tatctgtgtc gcctt
//
LOCUS       DAOSPM010000040        16524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_3532_length_16524_cov_14.111968, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000040 DAOSPM010000000
VERSION     DAOSPM010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 16524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16524
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3532_length_16524_cov_14.111968"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..1201)
                     /locus_tag="PLW25_04990"
                     /pseudo
     CDS             complement(290..1201)
                     /locus_tag="PLW25_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(1307..1960)
                     /locus_tag="PLW25_04995"
     CDS             complement(1307..1960)
                     /locus_tag="PLW25_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53880.1"
                     /translation="MLWRDGNRRVHDLVVYARQRFYSVGMTTNGTQPLDVPTDVLWVS
                     LDGLGETHNQLRGAPVFDSIMANVRASCHPRLFAHVTVNAINADEVPRLLVFLNESFH
                     AITLQFYYPYGQKDELFLDFDRREKLLDEVIRMKKKGIRVLNSVAALKALKRNTWTCR
                     DRLIDNANPDGTVQQGCYLRGRAEIDCTKCGFSPHTEISLAFQGNLRAIQSGMRIFW"
     gene            complement(2366..3214)
                     /locus_tag="PLW25_05000"
     CDS             complement(2366..3214)
                     /locus_tag="PLW25_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOS53881.1"
                     /translation="MIAVITDSTCDIPDPLLRDLGIEVVPQYVIWGDKQYRDRLDIEP
                     AAFYQRLSTEVEKPRSSQPGVMDFQQAYERAANNGADELVVVTISSTLSGTYENARRA
                     AQDFSLPVTVVDSTGTSMGMGWQVLAAARAARSGMDTRGILEKIDHVRRRITQIAGLD
                     TLEYLSKGGRIGAAVKWAGTMLKVKPLVAFNVLTNLVEPVGLVRTYTSMVETLYRKFF
                     ERVKGIANLRVAVLHGGARAQAEALAERVRAEYHPLELFVNSTGPVVGLHTGPGALGL
                     CGYMDE"
     gene            complement(3254..4513)
                     /locus_tag="PLW25_05005"
     CDS             complement(3254..4513)
                     /locus_tag="PLW25_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS53882.1"
                     /translation="MAVVEKSSANKPSVKNLPRNIWAVGFTSMFMDISSEMVMNILPL
                     FLSNVLGVQTSIIGLIDGIAEATASILKLFSGWLSDRLGGRKWLAVAGYGLSALVKPF
                     FYIANSWGLVAGVRWADRVGKGIRTAPRDALVADSVTPDVRGLAFGFHRAMDTAGAFI
                     GILIAALVVWLTQKSDVLLARSTFQIIVLASLLPALLAVLSLVVGAQDVAVQGQRAAP
                     KFSLRNMGKPFTIFMVIVSLFTLGNSADAFLVLRAQNLGVSVLGILIMLAAFNLIYTL
                     VSTPAGSLSDRIGRRRLIIGGWLVYAVIYFGFALARSSWQVWVLYALYGVYYGLAYGS
                     AHALVADLVPENLRGTAYGTYNAVIGLLAFPASFIAGVLWQGIGAWTGLGPSAPFFFG
                     GLLALLAALLMLFWMPAIHTVEEKVLH"
     gene            complement(4549..5502)
                     /locus_tag="PLW25_05010"
     CDS             complement(4549..5502)
                     /locus_tag="PLW25_05010"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOS53883.1"
                     /translation="MGDRYKNTSLHWAKRLIIAFHANQLLQEMIFMSEVTPTTRKTAS
                     IGDWLLRLIKGVIIGIGFITPGLSGGVLAVVFGLYEPLMRFIANLKEKFIKNLLFFIP
                     VGIGGIIGVVAFSAAVDWAFTHYAAPFIWLFIGFIAGTFPSLFKTAGKQGRKAWHWLL
                     LAVIAAGMFLMMKWMESIKNVTLAPSFLNWLMSGALIGLGVVVPGMSPSNFLIYLGLY
                     QPMANGIKNLDLGVILPLALGLILCVLVFAKLVSWLFNKAYTVMYHLILGVVVGSTLA
                     IIPAGVSGWTIALCAVLFLLGFAASFALAKVDEKHPHEQII"
     gene            complement(5773..6384)
                     /locus_tag="PLW25_05015"
     CDS             complement(5773..6384)
                     /locus_tag="PLW25_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS53884.1"
                     /translation="MTQHQAGEPNAENRPEPGHFRLLAPFYEHFIQPKAPDRILAHLG
                     LPQPGGVVLDAGGGTGRVAQFFAGKAAAVVVADQTFAMLREARKKEGLTGICALTEEM
                     PFRNEGFCCIIMVDALHHVADQEATIRELWRLLKPGGRIIIEEPDIRRFEVKLLALIE
                     KLVLMRSRFLSPGQVTALFNFPGASSRVEVAGWTAWIIIEKTA"
     gene            complement(6381..8165)
                     /gene="thrS"
                     /locus_tag="PLW25_05020"
     CDS             complement(6381..8165)
                     /gene="thrS"
                     /locus_tag="PLW25_05020"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOS53885.1"
                     /translation="MARQKEHYEDSQLYRIRHSTAHIMAQAVLEKFPEGKVAIGPAIE
                     DGFYYDFDLPRPLTPEDLEGIEKRMRGIIQSGVNFERKVVSADEAKVLFRNQPFKMEL
                     IEGLESGEMDDDGNPIDEKPEISIYSQGGFTDLCRGPHVTNSREINPAAIKLLNVAGA
                     YWRGDEKRPMLQRIYGTAWSKPEELKDYLWRLEEAKKRDHRKIGQELDLYSVNDEIGA
                     GLILWHPKGGRIRKLIENFWSEEHEANGYDFVYTPHIGKAQLWETSGHLGFYKDNMYS
                     PVDIEGQQYFLKPMNCPFHLHIFKNSLRSYRDLPLRYAEEGTVYRYERSGVLHGLMRV
                     RGFTQDDAHHFCRPDQMPAEIDFVLNFSLNILRSFGFSDIHAYLSTRPEKSVGDPAQW
                     EAAEAALEDSLNRSGLDYDVDRGGGAFYGPKIDLKVEDAIGREWQLSTIQFDFNEPER
                     FDLYYIGEDGQPHRPYMIHRALLGSLERFFGVLIEHYAGAFPVWLSPTQAAIIPIADR
                     HLDYARKVEAELRKAGLRVMVDDRPERMNAKIRDAQNQKIPYMLVVGDKEEESGQVAL
                     RLRNGENPGPLLLADFLTLAKSDIAAKK"
     misc_binding    complement(8207..8441)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8620..9327)
                     /gene="prxU"
                     /locus_tag="PLW25_05025"
     CDS             complement(8620..9327)
                     /gene="prxU"
                     /locus_tag="PLW25_05025"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558502.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(9079..9081),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOS53886.1"
                     /translation="MAENKDGCVKPVKGPISAASETPSAAPVFQKEVSMVQAKVGKPA
                     PDFELTAFHQGGFKNITLSDYKGKWIVICFYPGDFTFVUPTELTAVAVKYKELQALGV
                     EVLAISTDSRFSHKIWQEEELSKMVEGGVPFPLLSDAGGRVGTIYGVYDETAGVDIRG
                     RFLIDPDFNIRAMEVLTPEVGRNVSELIRQVQAFQHVVATGEVTPSGWVPGKPTLTPG
                     PDLAGKVWKVWKPDQAF"
     gene            complement(9505..12411)
                     /locus_tag="PLW25_05030"
     CDS             complement(9505..12411)
                     /locus_tag="PLW25_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOS53887.1"
                     /translation="MNPAQKSVQLTINGEKKALLVTPEETLLDALRAASYFSVKFGCG
                     SGECGSCTVIMNGKAVRSCTIKAMEAEGSQIITLEGLSKNGELDPIQQAFIETGAIQC
                     GFCSPALMLSAKALLDHQPEPSEKEIRNALNPVLCRCTGYVRGVNAVQRAAAIKRGEK
                     VAPFNHISLPLPDDLAQIEIPAEFFRADGNRDPLPPLVYTPASMPAARVVGKSEIKVD
                     ARKLALGKPVFTDDIHLPGMLYAAMLTSPHAHARIKHIDASRARALPGVHAVLTHHDV
                     PRVKYASGGQSYPQPLPYDQVSLDDKVRFVGDRVAVVAAESLEIANEALKLIDVEYEV
                     LPAVVDMEAAMQPGAPVIHDEPDTEGIYNRERNIVYHIEAEVGDVDKAFAESDHVFEG
                     EYHTQKQQHVHLEPHVAISYFDEDERMVIRTSTQVPFHTRRIIAPLIGLPLKKIRIIK
                     PRIGGGFGNKQEVLIEDLVAHLTMITKRPVRMEYTREQEFTSSRSRHATTIRYKIGVK
                     GDKVTAASLYLIGDTGAYATHGLTVTMVGGFKGLTLYNSPNSRFICDVVYTNTVVAGA
                     YRGYGSMQCQYGIEVLMSEIADRLGLDVVDFKKKNWIKVGEEMYLSQQLGEGRTGVRQ
                     ELVTSAMEQCVEIGRRVTDFDAKRAAYRDQTGRYRRGIGMAVVMHGSSVANLDMAAAT
                     LKMNDDGSFNLLMGATDLGTGSDTILAQIAAEVLNTDVDDIIVYSSDTDFTPFDKGAY
                     ASSTAYLSGEAVRKTAVNIKNQLLEHAGKMMGVKDLNTLRLEYKKVVAADGRQVSFEE
                     IGLSSLHQMAQHQIMATESHVSPVSPPPTAAQFVEIVVDTETGKVDTERLVMVVDCGR
                     VINPVTASGQVEGGMAQALGFALTEDMLYSAEGQPLNPNLVTYRVPRAVEMPVMDVIF
                     VQTDEPTGPYGVKSVAEISIDGVAPAMASAIHNAIGVWLRDLPYTKEKVKKAIG"
     gene            complement(12754..13482)
                     /locus_tag="PLW25_05035"
     CDS             complement(12754..13482)
                     /locus_tag="PLW25_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOS53888.1"
                     /translation="MSIYTDLLALLPKEPVPLRSVLVGVHWTLVCSKHSGLGSTLVNY
                     GPHGHAPMRDVGCLLEKSAQELAGWIESDNLLEASLGIAAYNSLIDLDDSKLKEGNAS
                     EVLRCECPGKNLAVIGHFPFITKLRDLAANLWVIEKDPHGDDFPEEAAQEFLPSADVV
                     AITGTAFINHTIERLLSLCRPGVPVMILGPSTPLVPLLFDYGVTYLSGSRIIDEAAAR
                     LTISQGAGFPQVRGVQVVNMVKDA"
     gene            complement(13503..14327)
                     /locus_tag="PLW25_05040"
     CDS             complement(13503..14327)
                     /locus_tag="PLW25_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOS53889.1"
                     /translation="MLNILLLSLMASLGTGLGGLIAIIRRPGRRSYGLLMGITAGVMI
                     SLAFLELVAEAWKQAGYITATIGFATGAIFMFLLDHFAPHIRFGEKEVRGEPPCAEPR
                     QRMGPRPGGRGFGRLYRQGRMYDYKLINTGLLLAVGITLHNLPEGISIGAGYLHNPKF
                     GLFIAVAILLHNIPEGIATALPLCKGGVCRWDAFRVAFLSGLAEPVGALLASLFLVNF
                     QGLVPAALAFAGGVMVFITLDEMIPTAREYGHEHYTAIGIILGSIFVFLLSGIFGV"
     gene            complement(14444..14887)
                     /locus_tag="PLW25_05045"
     CDS             complement(14444..14887)
                     /locus_tag="PLW25_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOS53890.1"
                     /translation="MKIIYEDLQRIIQAHARFLAGECDGTRANLEGIDLYGKDLANVN
                     LAGANLKSTNLKRSILWDANLEEADLEQAAFKHAILQGANLRKANLRGAGLMGVNLIG
                     ANLSGADLTGADLDCAVLQGALYDKTTQWPEGFDPLSAGAICQDE"
     gene            complement(14960..16000)
                     /locus_tag="PLW25_05050"
     CDS             complement(14960..16000)
                     /locus_tag="PLW25_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOS53891.1"
                     /translation="MTLPTDPDQGTEHTTTEMMVGQRLRKLRVSKGLSLRSLADLSGL
                     NINTLSLVENAKTSTSVGTLQQLAAALSVPLSAFFVEETIEKQVVFTPAHPQPTTAFN
                     HTQLVYLAQNLSRKTIQPYLVTLKAGMGSGNNMIVHTGHEFVYCIEGQAQFTIDNEVY
                     PLKTGDSLIFEAHLPHAWENNQPALARLLMILAPADAAEEAGSRHVSLEKLQKEKTMK
                     IAVITEDGKKISQHFGRAPYFLVLTIEDGKVTHREMREKPGHGHFGGRAEETHGHRHG
                     FDEGSHLKHKSMAETISDCKVVLCGGMGMGAYDSMRRLEITPIVTDLKDIDEAVKLYL
                     EGKLVDHPELLH"
     gene            complement(16005..16439)
                     /locus_tag="PLW25_05055"
     CDS             complement(16005..16439)
                     /locus_tag="PLW25_05055"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOS53892.1"
                     /translation="MPNKNVSVEAVLLEGYKIEVKSRQHVSIVDQPPAGGGGDAGPTP
                     LEYLFVSLAGCVATIGQIIAKQQQLPLRGISVKIDGDLDTDVLMGKRSDVRAGFEGIR
                     VKVAIDGDMTQQEKEQFVRDIDARCPISDNILNQTPIKFAVE"
BASE COUNT         3886 a         4678 c         4220 g         3740 t
ORIGIN      
        1 tcacctgaac attctcgaac aaccggagac agtggctgcc atccgagcct acctgaaaac
       61 agccagctga tccttgacgc ggtaaaaggg aagtgcgcaa atgatgaata aaacgcgcgc
      121 ttctatcctc caaaatacta agggaaatga gaggttttta gaacgcgaat tttccaggac
      181 gcgggtttgt acgtcgttcc ctcaaccacg atgacatccg cgctctcaat ggagccgcga
      241 cctgttgcgc accctgctaa gccttcagaa acgtatctta cgaaaatctc tataggggct
      301 cgaggttgcc taacctcatc gtcatggcaa tggcgcccag cacccccgag tatctcccca
      361 gcgcgggagg gacaatatat tcacccatgc cagaaaggat agcgtcctcg cgaatgtaac
      421 cgtttagcag ctcagtgacc ctgctcctga tgagcgggaa taagaataac cgctgcataa
      481 ttccgccccc taacacgatg atgcgtggag aaagcgtgag gatcaaattg gtcagcgcgc
      541 tggcgatata ctccacctcc agcagccaga acggatcctc atccgtcagg tcttcaccac
      601 gtttgccaaa acgggcagtt atcgctggtc cggcagccag accctcaaaa cagtcccggt
      661 ggtacgggca gctgccttca aacgggtcct tcagcctgtc atgagggatg cgtatatgcc
      721 ccatctcaac cgccgccggc cctgatagcg gtgcgccgtt caacaggtac gcgccaccaa
      781 tcccggttcc cactgtgaga tagggggaag ggtcaattcc cctggacgcg ccccagatat
      841 actccccgat ggcggagccg ctgacatccg cgtggaaggc aatgggcacg cccaattact
      901 gctgaagttc accaaggatg ttgtattctc tccatcctgg cttgggcgtg gtggtaatgt
      961 agccataagt cggggaaagg ggattcacct ccacagggcc aaaagaggcg agcccgatac
     1021 ccgcaaccat cccctgctta ataaaaggct caaagaaaag gcgcacctgt tccagtgtgc
     1081 tggagggatc ggttgtactg aaggtcactt gatgatccaa ccgtggaggt tctgaggcca
     1141 gcgcgcagat gaatttcgtt ccgccagctt cgataccgcc atagagacgt tcgtcagtca
     1201 tctaactcct taatgcccga aatctggtac acgcattata acttccgagt tttcaatggg
     1261 caatcaggct tcgaaattgg ttagtggttt cctgtaactt tattactcac caaaagatcc
     1321 gcattcccga ctggatggcg cgcaggttcc cctggaacgc cagcgaaatc tcggtgtgtg
     1381 gagaaaaccc acattttgtg cagtcaatct ctgctctgcc gcgcaggtaa caaccctgct
     1441 gtacagtgcc atctgggttg gcattgtcga tgagcctatc acggcaggtc caggtattgc
     1501 gtttgagcgc cttgagcgcg gctactgaat tgagcaccct gatccctttc tttttcatcc
     1561 ggatgacctc atccaggagt ttttcacgcc tgtcaaaatc aaggaacagc tcatcttttt
     1621 gcccatatgg gtaatagaat tgcagggtga tggcgtggaa agactcattt agaaacacca
     1681 gcagccgcgg aacctcatcc gcattgatgg cgttaaccgt aacatgggca aaaaggcgcg
     1741 ggtggcagct ggcgcgcacg ttcgccatga tggagtcaaa tactggcgcg ccccgcaatt
     1801 gattgtgtgt ctcacccagc ccatccaagc tcacccaaag cacgtccgtg ggcacatcca
     1861 gcggttgggt gccattggtg gtcatgccca cgctgtaaaa acgctgccgc gcgtaaacca
     1921 ccaggtcgtg cacgcgccgg tttccatcgc gccacaacat tggttctcct ccttcgaaaa
     1981 ttaaaacacg gttaccgcgc gcgtaaagcc gatcgatggt cgcgcaggct tggtcataat
     2041 ttggctcaga ggtttttagg aggtgataag ggcattggcg gcaggtgaga ttacaacgat
     2101 aagtcaattt atagctcgcc agaaagggtc gctttcgacc cagccagtag tgcaaaaaaa
     2161 tgtagatagc gcgcctggtc aaccctttta gatcagtatg aaaatgaggc atataggtat
     2221 tgtatcaagc ccgagcgcct ggcgtcatgc caaaggaaag tgtgggtcag cagtaagctg
     2281 ctggcggatg aactgatcca cgcaggcgtc aaccttggaa ccccacatgg caattaaccc
     2341 gtcatgtacc tgcgctttcg ttcactcatt cgtccatata accacaaagt cccagcgcgc
     2401 cggggccagt atgcagcccc accaccgggc ccgtggagtt gacgaaaagc tccaatgggt
     2461 gatattctgc gcgcactcgt tcagccagcg cttcagcctg cgcccgcgct ccgccgtgta
     2521 acaccgccac gcgcaggttc gcaatccctt taacccgttc aaaaaatttt cggtacaggg
     2581 tttcaaccat ggaggtatac gttcgcacca gcccaacagg ctctaccagg ttggtaagca
     2641 cattgaacgc gaccagcggc ttgaccttca acatggttcc cgcccattta accgcggctc
     2701 cgatgcggcc gcccttgctc aggtattcaa gcgtgtccag tccggcgatc tgggtgattc
     2761 tgcggcgcac atggtcgatc ttctccagaa tgcccctggt gtccatgccc gatcgcgcag
     2821 cgcgggcagc cgccagcacc tgccatccca tgcccatcga agtgcctgtg gaatccacca
     2881 cggtaaccgg caaagagaag tcctgcgccg cccgcctggc gttctcgtac gttccactga
     2941 gcgtgctgct gatcgtgacc actaccaatt catcagcgcc attattagcg gctcgttcat
     3001 aagcctgctg aaaatccatc acacccggtt gcgaagagcg gggcttttca acctctgttg
     3061 ataaacgctg gtaaaacgca gcgggttcaa tatccagccg gtcgcggtat tgtttatctc
     3121 cccaaatgac gtattgcggc accacttcaa tccccaaatc ccgtaaaaga gggtcaggaa
     3181 tgtcacaggt gctgtcggtg atgaccgcga tcatgggact attttacacc ggtaatgtga
     3241 taaagattat ttatcaatgg agtactttct cttccaccgt atgaattgcg ggcatccaaa
     3301 agagcatcaa cagggctgcc aacaaggcca gcaagccacc gaaaaagaac ggcgctgacg
     3361 ggccaagccc cgtccaggcg ccgatgccct gccaaaggac gccggcaatg aaagatgcgg
     3421 ggaacgccaa cagaccgatg accgcgttgt acgtgccgta ggctgttccg cgtaagttct
     3481 caggaacaag gtcagccacc aaggcatgag ccgaaccgta tgccagccca taataaaccc
     3541 cgtacagcgc atagagcacc cacacctgcc agctggaacg cgccagcgca aagccaaaat
     3601 aaatgaccgc gtacaccagc cagccgccaa tgatgagccg tctcctgcca atgcggtcag
     3661 atagcgaacc cgcaggagtg gataccaacg tgtaaataag gttaaacgct gccagcatga
     3721 tgagaattcc cagcaccgaa acgcccaggt tctgcgcgcg cagcaccaaa aaagcatctg
     3781 cagagtttcc cagggtgaac aggctgacaa tgaccatgaa aatggtaaag ggtttgccca
     3841 tattgcgcag cgagaatttc ggcgcggcgc gctgcccctg aaccgccacg tcttgggcgc
     3901 cgaccaccag cgaaagcacc gccaggagcg ccggcagcag gctggccaga acgataatct
     3961 gaaaagtcga acgtgccaaa agcacgtcgc ttttttgcgt cagccacacc accagtgcgg
     4021 cgatgaggat accaataaag gcgccggcag tatccatggc gcggtgaaaa ccaaacgcca
     4081 ggccgcgaac atcaggggtg acagaatctg ccaccagcgc gtcacgcggg gcagtacgga
     4141 tacccttgcc cacgcggtca gcccagcgca cccctgccac cagcccccag ctgttggcaa
     4201 tgtagaagaa tggcttcacc agggcagaaa gaccataccc cgccaccgcc agccatttgc
     4261 gcccacccag gcggtcagaa agccaaccag agaacaactt gaggatactg gcagtggctt
     4321 cagcaatgcc atcaatgagc ccgatgatgc tggtctgcac tcccagcacg ttggaaagaa
     4381 aaagcggcag gatattcatg accatttcgc tggaaatatc catgaacatg ctggtaaaac
     4441 ccactgccca tatgttacgc ggtaggtttt taactgaagg cttgttagca gaactctttt
     4501 caacaactgc catcccgggt ctcactcttc ctgaaaaggg gaaaataatt agatgatctg
     4561 ttcgtgcgga tgtttttcat ccaccttggc caacgcgaag gatgcagcaa aaccgagcag
     4621 gaacaacacc gcgcacagcg cgatggtcca tccgctcacc cctgccggga tgatcgccag
     4681 cgttgaaccc accaccacac ccagaatcag gtgatacatc acggtatagg ctttgttgaa
     4741 caaccacgag accagtttag caaaaaccaa cacgcacagg atcagcccaa gcgcgagagg
     4801 gagaatgacc cccaggtcca ggtttttaat gccgttggcc atcggttgat ataaacccag
     4861 gtagatgagg aagttagaag gactcattcc aggcaccacc acacccagcc cgataagcgc
     4921 gccgctcatc agccagttga ggaagctcgg cgccagggtg acgttcttga tgctttccat
     4981 ccatttcatc atcaagaaca taccggcggc tatcaccgcc agcaggagcc agtgccaggc
     5041 tttgcgccct tgcttgccgg ctgttttaaa gagcgaaggg aaagtaccgg cgatgaaacc
     5101 gataaaaagc cagatgaacg gcgcggcgta atgcgtgaaa gcccagtcca ccgccgccga
     5161 gaacgctacc acaccgatga tcccgccaat acccaccggg atgaagaaaa gcaggttctt
     5221 gatgaatttt tccttcaggt tggcgatgaa acgcatcaac ggttcataaa gaccaaatac
     5281 caccgccagc acaccgcctg aaagacccgg ggtgatgaag ccgatgccga tgatgacgcc
     5341 ttttatcagg cgcagcagcc agtctccaat cgaagcggtt ttcctggtcg tgggtgtaac
     5401 ctctgacata aaaatcatct cctgtaaaag ttgattcgca tgaaatgcta ttataaggcg
     5461 ctttgcccaa tgtagtgagg tgtttttata ccgatctccc aatgccagtg cccttcgcgc
     5521 tgtgtggtca atgaacagga aggttcactc cctcgcccag ccctctgctg ccagtacagg
     5581 cagggcggca gaacaggcaa aaaaaaaaca gcaggataat cgaagcgcac accccagagt
     5641 tcagagcccg ctgaaatgtg cgcaagccac gccatgcagc catccataga ggtggataaa
     5701 gcccttgtac gcgtcagcct aaaagtcctt ccctcgtcag gatggacttt ttaagcgcca
     5761 ttcatgcatg ggttatgcgg tcttttcgat gatgatccag gcagtccagc ccgcgacttc
     5821 aacacgtgag gaagccccag ggaaattaaa gagcgcagtc acctgacctg gtgagagaaa
     5881 tcggctgcgc atcagcacca gcttttctat cagcgccagc agtttcacct caaaacggcg
     5941 gatgtcaggt tcttcaatga taatgcgtcc gcccggttta agaaggcgcc ataactcacg
     6001 gatggtagcc tcctggtctg ccacgtgatg cagcgcatcg accatgatga tgcagcagaa
     6061 accttcattt ctaaagggca tttcttcggt cagcgcacat atcccggtca acccctcttt
     6121 ttttcgcgct tcccgcagca tcgcgaaggt ctggtctgcc accaccacag ccgccgcttt
     6181 tcccgcgaag aactgggcaa cacgcccggt gccgccgccg gcatccagaa ctacaccgcc
     6241 tggctgcggc aaccccaggt gggcaaggat cctgtcaggc gccttcggtt ggataaaatg
     6301 ctcgtagaaa ggagccagca gcctgaaatg accgggttcc ggcctgtttt ccgcgtttgg
     6361 ctcccctgcc tggtgttgag tcattttttg gcggcgatat ctgatttcgc cagcgtaaga
     6421 aaatctgcca gcagaagtgg accagggttc tcgccattgc gcaagcgcag cgccacctgc
     6481 ccgctctcct cttctttgtc gccgacgacc agcatgtaag ggatcttctg gttctgggca
     6541 tcgcgaattt tggcgttcat gcgctcgggt cggtcgtcca ccatcaccct caacccggcc
     6601 ttgcgcagct cagcttcaac cttgcgggcg taatccaggt ggcggtcggc aatgggaatg
     6661 atggctgcct gggtaggtga aagccaaaca ggaaaagcgc cagcataatg ttcgatgagc
     6721 acgccaaaga agcgctccag gctgcccaac agcgcgcggt ggatcatata agggcggtgt
     6781 ggttgaccat cttcgccgat atagtacagg tcaaagcgct cgggctcgtt gaagtcgaac
     6841 tggatggtcg agagctgcca ctcgcgaccg atggcgtctt ccactttgag atcgatctta
     6901 ggtccgtaga aagccccgcc gccgcggtca acgtcgtagt ccaatcccga gcggttgagc
     6961 gaatcttcca gcgctgcctc ggcagcttcc cattgcgccg ggtcacctac tgatttttcg
     7021 gggcgagtgc tgagataggc atggatatcg gaaaagccaa aactgcggag gatattcagg
     7081 ctgaagttaa gcacgaagtc gatctccgcg ggcatttggt ctggtcggca aaagtggtgt
     7141 gcatcatcct gggtgaaacc acgcacgcgc atcaacccat gcagcacgcc gctgcgttca
     7201 tagcggtaca cggtgccttc ctctgcgtaa cgcaggggca ggtcgcggta agaacgcagc
     7261 gaatttttga aaatgtgcag gtggaagggg caattcatcg gcttcaagaa atactgctgc
     7321 ccttcaatat cgaccggtga gtacatattg tctttataaa agcccaggtg accgctggtt
     7381 tcccacagct gcgccttgcc gatatgcggg gtgtaaacga aatcataccc attggcttca
     7441 tgctcttccg accaaaagtt ctcaatcagt tttcgaatgc gcccgccctt tgggtgccag
     7501 aggatcaacc ccgcgccaat ctcatcgttc acactgtaaa ggtcaagctc ctgtccaatc
     7561 ttgcggtggt cacgcttctt ggcctcttcc agccgccaca ggtaatcctt caattcttct
     7621 ggcttgctcc aggcggtacc gtagatgcgc tgcagcatag gacgtttctc atcgccgcgc
     7681 cagtaagctc ctgcaacatt gagcagcttt atcgcggcgg ggttgatctc gcgtgaattg
     7741 gtcacgtgcg gtccgcggca cagatcggta aagccgccct gcgagtagat ggaaatctcg
     7801 ggtttttcat ctatcggatt accgtcatca tccatctcac ccgactcgag gccttcaatc
     7861 agttccatct tgaaaggctg attacggaat agcactttgg cctcatccgc gctcaccact
     7921 ttgcgttcaa agttgacccc cgattggatg atgccgcgca tacgcttttc gataccctcc
     7981 aaatcttcgg gggtaagcgg tcggggcagg tcaaaatcat aataaaaacc atcctcaata
     8041 gcgggaccga tggccacctt cccttcaggg aatttttcca gtaccgcctg cgccatgatg
     8101 tgcgcggttg agtggcgaat tctgtacagt tgcgagtctt cgtagtgttc cttttgtctt
     8161 gccattcttt cctccattcc aagattaaac aaaaacgccc gtcccatact ggggcgagag
     8221 ttgcctgctt cgcggttcca cccagattaa tgcggagaaa aaccgcatca tctcttgtga
     8281 ccattaacgg agtcatccgt ttcccttaat aggcgagtgc cgttcaggtt caagctcgcg
     8341 ggtggttttc gctgttggtg ttcctgaaag aactttcagc cagtggttcc ttctctctga
     8401 gagaacggtc aacaggtact cgtcccggtc agcgccttgt tgaatgaagg taattatact
     8461 ccaatttttt tcaaacctaa ggagaatttc gggcggaata gtcgctgaac cttccaacct
     8521 tgttttgctg ccgggtttcc caacccacgc ataaaaagca ggaggccgcg gggttctcgc
     8581 cagcctcctg ctgaaatacg ccggatgaca cgaataaggt taaaacgcct ggtcaggctt
     8641 ccaaaccttc caaaccttac cagccagatc tgggcccggt gttaaggttg gcttgccagg
     8701 tacccaaccg gatggcgtga cttctccggt cgctaccaca tgctggaaag cctgcacctg
     8761 gcggatcaat tcagacacat tacgacccac ttcgggggtc aaaacttcca ttgcgcgaat
     8821 attgaaatca ggatcgatca agaatctccc ccggatgtcc actcctgcgg tttcatcgta
     8881 gacgccgtaa atagtgccta cgcgcccgcc agcatctgag agcaatggga atggcacgcc
     8941 gccctccacc atcttggaga gctcctcttc ctgccaaatt ttatgcgaaa agcggctgtc
     9001 agtgctgatt gccaggactt ccacccctaa tgcctgtaat tctttgtatt tgacggcaac
     9061 tgccgttaat tctgtcggtc agacaaaggt gaaatcaccg gggtagaaac aaattacaat
     9121 ccatttaccc ttgtaatcag aaagggtaat attcttgaac ccgccctggt gaaaagcagt
     9181 taactcgaaa tcaggcgcgg gtttgccaac ttttgcttga accattgaca cctccttttg
     9241 aaatactggc gcagctgagg gggtttctga tgcagcggaa attggacctt tcactggttt
     9301 aacacaacca tctttatttt ctgccattca acctcctttt ttttgccggc gtaattcttg
     9361 cgtgtaattt aatcatacta agatctctca aaaaatcaat cgccatcctt cttcgaccca
     9421 acgtgggggt tagaatatcg agttccaatg gaaaacccag cttaccgaac cctcgccagc
     9481 gggtttggca gccagttcca cgggttagcc gatggccttt ttgacctttt ccttggtgta
     9541 cggcaaatca cgcagccata cgccaatggc gttgtggatg gcgctggcca tggctggcgc
     9601 aactccatca atggaaattt ccgcgactga tttcacaccg taaggaccgg tgggctcgtc
     9661 agtttgcaca aagatcacat ccatcaccgg catctcaaca gcacgcggga cgcggtaggt
     9721 cacgaggttg ggattgagcg gttgaccttc agcagaatac agcatatcct cggtaagggc
     9781 aaaacccagc gcctgggcca tgccgccctc tacctgcccg gaggctgtta cagggttgat
     9841 aacacgaccg caatccacca ccatcaccag ccgttctgta tccactttgc cggtttcggt
     9901 atccaccacg atctcgacaa attgcgcggc ggtgggcggc gggctcaccg ggctaacgtg
     9961 cgattcggtg gccattattt gatgctgcgc catctgatgc aggctgctga gaccaatctc
    10021 ctcgaagctc acctgtcgac catctgctgc aaccactttt ttatactcca ggcgcagagt
    10081 gttgaggtct ttcacgccca tcatttttcc tgcgtgttcc agcagttgat tcttaatatt
    10141 gacggcagtc ttgcgcaccg cttctccgga aaggtaagcg gtgctgctgg catacgctcc
    10201 tttatcaaat ggggtgaagt cagtgtcaga tgagtagacg atgatgtcat ccacatcggt
    10261 gttgagcact tcagccgcga tctgcgcgag tatggtatcc gagccagtgc ccaggtcagt
    10321 cgctcccata aggaggttaa acgaaccatc atcgttcatc ttgagcgtgg cagcggccat
    10381 atccaggttg gccacgctgc tgccgtgcat caccaccgcc atgccgatgc cccgtcggta
    10441 gcgacccgtt tgatcgcggt aagcagcgcg tttggcatca aaatcagtga ccctgcgccc
    10501 aatttccacg cattgttcca tggcgctggt aaccagctcc tggcgcacgc ctgtgcgacc
    10561 ctcgccaagt tgctgcgaaa ggtacatctc ttcgcctact ttgatccagt tctttttctt
    10621 aaagtccacc acatccagcc ccagcctatc ggcaatttcg ctcatgagca cttctatgcc
    10681 atactggcac tgcatgctgc cgtaaccgcg gtaagcccca gccacaacgg tattggtata
    10741 aacaacatca caaataaatc gggagttggg tgaattgtac aacgtcagcc ctttgaaccc
    10801 gcccaccatg gtgacggtga gcccatgagt ggcatacgcg ccggtatccc cgatcaggta
    10861 cagactggct gcagtgactt tatccccttt cacgcctatc ttatagcgga tggtggtggc
    10921 gtggcgtgag cgcgagctgg tgaactcctg ctcgcgggtg tactccatgc gcaccggtcg
    10981 tttggtgatc atggtcaaat gagcgaccag gtcttcaatg agcacttcct gcttgttgcc
    11041 gaatccgccg ccgatgcgcg gcttgatgat cctgatcttc ttcagcggca gaccgatgag
    11101 cggcgcgata atgcggcgcg tgtggaaagg aacttgcgta gaggtacgga taaccatgcg
    11161 ttcgtcttca tcaaaataac tgatggcaac atgcggttca aggtgaacat gctgctgttt
    11221 ctgggtgtgg tattcgcctt cgaacacgtg gtcagattca gcaaaagctt tatctacatc
    11281 acctacttca gcttcgatat gataaacaat gttgcgctct cggttataaa tgccctccgt
    11341 gtcaggttcg tcatggatta ccggcgcacc tggctgcatg gcggcttcca tatctacgac
    11401 cgctggcagt acttcgtact cgacatcaat cagtttgaga gcctcgttcg caatttccaa
    11461 agactccgcc gccaccacag ccacccggtc gccgacgaag cgcaccttgt catccaggct
    11521 gacctggtca taaggtagcg gttgtgggta gctctgcccg ccgctggcgt atttcacacg
    11581 cggtacatca tggtgggtga gcacggcgtg cacacccggc aaggcgcgcg cgcggctggc
    11641 atcaatatgt ttgatgcgcg catgggcgtg cggactggtc agcatggcgg cgtaaagcat
    11701 acccggtaaa tgaatatcat cagtgaaaac tggcttgcca agcgccagct tgcgcgcatc
    11761 cacttttatc tctgatttgc ccaccaccct ggcggcgggc attgaagcgg gtgtgtaaac
    11821 cagtggcggg aggggatctc gattgccatc agcgcgaaag aactcagccg gaatttcaat
    11881 ctgcgccagg tcatctggaa gaggaaggct aatatgattg aaaggcgcca ctttttcacc
    11941 acgtttgatg gctgccgccc gctgaaccgc gttgacgccg cgaacatagc ccgtgcagcg
    12001 gcagagcacc gggtttaggg cattgcgaat ctccttctcg gatggttcag gctgatgatc
    12061 cagcaacgct tttgccgaaa gcattaatgc cggtgagcaa aagccgcact ggatggcgcc
    12121 ggtttcgata aaagcctgct gaatgggatc gagctcgcca ttcttactca agccttccag
    12181 ggtgatgatc tggctgcctt cggcttccat ggctttaatg gtgcaactgc gcaccgcctt
    12241 gccgttcata atcacggtac aagaaccgca ttccccgctg ccacagccaa acttgacgct
    12301 gaaatacgag gcggcgcgta gggcgtccag caaggtttct tccggtgtca ccagtaatgc
    12361 cttcttttca ccattgatgg tcagttgtac gcttttttga gcaggattca tggttcctcc
    12421 aaacgcgggt taacgccgat caggattttt attgacaact ctgaatgcgt tactatttta
    12481 ttatagaatc caaaaaatga cctgccgcgg ttggcgcggt ttcagtgctt ttccattctg
    12541 gcaatcagga ttagggaaaa ttccaccttg gaaactcaaa aacaagcagc accaaacccc
    12601 catgtggttt tgtccctgct tcactgggcg tataaacccc gcgactaccc cccgttcccg
    12661 cggtttccac aatggaagtt cgcgcctgtt tcaggtcaga gcggatggat aaactgaagt
    12721 ttgcgcattt aagttggtgg aatacggggt aactcaggcg tcttttacca tgttcaccac
    12781 ctgcacgccc cgcacttgcg ggaaaccagc gccctgactg atggtgagcc tggcagccgc
    12841 ttcatcgata atgcgcgaac ccgagaggta agtgaccccg tagtcgaaca acaatggtac
    12901 cagcggggtg gagggaccga gaatcatcac cggcacgcct ggacggcaca aggagagcaa
    12961 cctctctatg gtgtggttga tgaaagccgt gccggtaatg gcaacaacat ccgcgctcgg
    13021 cagaaattcc tgcgcggctt cttctgggaa gtcgtctccg tgggggtcct tctcaatgac
    13081 ccacaggttc gcggcgaggt cacgcagttt cgtaataaag gggaaatgcc cgatgacagc
    13141 caggttctta ccggggcatt cgcatcttaa cacttctgaa gcgttccctt ctttcagctt
    13201 ggaatcgtca aggtcaatta aggaattgta agcggcgatg cccaggctgg cttcaagcag
    13261 gttgtcagat tcgatccagc cggccaattc ctgagcgctc ttttctaaca ggcagccaac
    13321 gtcgcgcatg ggcgcgtgcc catgcggtcc gtagttgacc agggttgacc ccagtccact
    13381 gtgtttactg caaaccaaag tccagtgaac ccctaccaga acacttcgca gtggtaccgg
    13441 ctctttgggc agcaacgcca ataaatcagt atagatgctc atctcaacta ttcaggcgca
    13501 actcacacgc caaaaattcc tgacagcaag aaaacaaaga tcgaaccgag aataatgcca
    13561 atcgcggtgt agtgttcatg cccatattca cgcgcggtcg ggatcatttc atccagggtg
    13621 atgaacacca tcaccccccc agcgaaagcc agtgctgccg gcaccaatcc ctgaaaattc
    13681 accaaaaaca ggcttgccag cagcgcgcct accggttccg ccagaccaga aaggaaagcc
    13741 acccggaatg cgtcccagcg gcaaacgccg cccttgcaca acgggagcgc ggtggcaatg
    13801 ccttcaggga tgttatgcaa taaaatagcc acggcgatga ataaaccaaa tttggggttg
    13861 tgaaggtaac cagcgccaat cgagatacct tcaggcaggt tgtgaagcgt gatgcccacc
    13921 gccagcagca gaccagtgtt gatgagttta tagtcgtaca ttctgccctg gcggtaaagg
    13981 cggccaaacc cgcgccctcc agggcgcgga cccatccttt ggcgtggttc agcgcaagga
    14041 ggttcacctc ttacttcctt ctcaccaaaa cgtatatgag gggcgaagtg atccagcagg
    14101 aacatgaaaa tcgctcctgt ggcaaaacca atggtggcag tgatgtaccc cgcctgcttc
    14161 caggcctcgg caacgagctc cagaaaagcc agggaaatca tcacacccgc ggtgatgccc
    14221 atcagcagac cataagacct tctgcctggt cgtcggataa tcgcgatcaa cccgccgagc
    14281 ccggttccca aactggccat caggctgagg agtaagatgt taagcaaggg ggattgtccg
    14341 aaaatcataa attccttaat aaggaccatt catcaccatg gattaatgaa gtgccattgt
    14401 aaccgaaatg cacattcgtt ccaaggcgat ccgttgatca tggtcactca tcctggcaaa
    14461 tggcgcctgc cgaaagcgga tcaaagccct caggccactg ggtggtttta tcatacaacg
    14521 ctccctgcaa aacagcgcaa tccaggtccg cgccggtaag gtctgcgccg ctcaggtttg
    14581 cgccgatgag gttcacgccc atcaaacccg cgccacgcag gttggccttg cgcagattcg
    14641 ccccctgtaa aatcgcgtgt ttaaaggcag cttgctcgag gtcagcctct tccaggttgg
    14701 catcccacaa aatggaacgc ttcaggttgg tgctcttgag attagctccc gcaagattca
    14761 catttgccag gtctttgcca tacaggtcaa tcccctccag gtttgccctg gtgccatcgc
    14821 actcgccggc gagaaacctg gcgtgcgcct ggatgattct ttgcaggtct tcgtaaatga
    14881 ttttcatcga gtgggtcact ccgtttgctt atgcggcgtc tcttatccta cagtaactta
    14941 gtcataaacg tgaatacatt taatgaagca gttcaggatg atccaccaat ttgccttcga
    15001 ggtataactt cacggcttcg tcaatatcct tcaaatctgt cacgatgggt gtgatttcca
    15061 ggcggcgcat actatcataa gcgcccatgc ccatacctcc gcagagaaca actttgcaat
    15121 cagaaatcgt ttctgccatg cttttatgtt ttaagtgcga accttcatca aagccgtgcc
    15181 gatgaccatg ggtttcttcc gcgcgaccac caaaatgtcc gtgcccgggt ttttcacgca
    15241 tttctctatg agtcaccttg ccatcttcaa tcgtcaacac caggaagtaa ggagcccgcc
    15301 caaagtgctg gctgatcttt ttaccatctt ccgtaataac cgcaattttc atggttttct
    15361 ccttttgtaa tttttcaagc gaaacatgcc ggctgcctgc ctcttcggct gcatctgccg
    15421 gcgccagaat catcagcaaa cgggcgagcg cgggttgatt attttcccag gcgtggggga
    15481 gatgcgcctc aaaaattagg ctatctccgg tttttaatgg ataaacctca ttgtcaattg
    15541 tgaactgcgc ctgcccctcg atacaataca caaattcatg cccggtatgt acgatcatat
    15601 tatttccgct gcccatgcct gcctttaagg tcacaaggta tggttgtatg gtttttctgg
    15661 aaaggttttg cgccaagtac accagctggg tatggttgaa tgctgtcgtt ggctgagggt
    15721 gcgcgggtgt aaaaaccacc tgtttttcga tggtttcctc aacgaaaaac gcagacaaag
    15781 ggacagatag ggcagccgcc agctgctgca gcgtgcctac agaggtagag gtttttgcgt
    15841 tttccacaag tgagagcgtg ttgatgttca acccgctcag atccgccagg gaacgtaacg
    15901 aaagcccttt gctcacacgg agcttcctga gccgttgtcc caccatcatc tcagtggtgg
    15961 tgtgttcggt tccctggtca ggatctgtcg gaagagtcaa gtttttactc cacggcgaac
    16021 ttaataggtg tttgattgag gatgttatct gagatcgggc agcgggcgtc aatatcgcgg
    16081 acaaattgct ctttttcctg ctgggtcatg tctccgtcga tcgcaacctt cactctgatt
    16141 ccttcaaacc cggcgcgcac gtcgctgcgt ttgcccatca gcacgtctgt atccaaatcg
    16201 ccatcgattt ttacagagat cccgcgtaaa ggaagttgct gctgtttggc aatgatctgg
    16261 ccaattgtgg caacacaccc tgccaatgaa acgaacaggt actccaacgg ggtggggccg
    16321 gcatctcccc cgccaccagc agggggttgg tctacaatcg aaacatgctg tcttgatttg
    16381 acttcaatct tgtatccttc caacaacact gcttcaacgc ttacattttt atttggcatt
    16441 ctaacctcac gtataaaaag atgtatgaaa aatgaatcct ccccgcagca agctgcgggg
    16501 tatcgtcttt agattttttc cttg
//
LOCUS       DAOSPM010000041        16367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_3574_length_16367_cov_12.983877, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000041 DAOSPM010000000
VERSION     DAOSPM010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 16367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16367
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3574_length_16367_cov_12.983877"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..858)
                     /gene="truA"
                     /locus_tag="PLW25_05060"
     CDS             complement(91..858)
                     /gene="truA"
                     /locus_tag="PLW25_05060"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOS53893.1"
                     /translation="MALYKLILAYDGTDFSGFQRQGKTRTVQLVVETVLRELGWQQPA
                     MLFAGRTDAGVHACGQVATISLDWQHSSADLVNALNARLPDDVSVIEANVAQAEFHPR
                     YDASSRTYHYHLYQLPVRQPLLERYAWRIWPELDPERLDRAASRFIGRHDFCAFGRAM
                     KKGGSTTREVYTSQWMKTPEGWRYEVEANAFLYHMVRRMVFIQVQAASGADESEIETA
                     LREGRPLKPGLAPAQGLFLVNVSYAGLTRERCEDEKN"
     gene            complement(886..1263)
                     /gene="rplQ"
                     /locus_tag="PLW25_05065"
     CDS             complement(886..1263)
                     /gene="rplQ"
                     /locus_tag="PLW25_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOS53894.1"
                     /translation="MRHQIAGSKLGRQKDHRAGLRRTMVKQLFEHERIQTTHTKALFI
                     RGEAEQLITLARRSNSGTDAEKVHARRLAAARLGSAAMVRKLFDEIAPRYENRKGGYT
                     RIYKLGPRLGDSAMMVQIELVEE"
     gene            complement(1270..2235)
                     /locus_tag="PLW25_05070"
     CDS             complement(1270..2235)
                     /locus_tag="PLW25_05070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOS53895.1"
                     /translation="MCALINMVTPKIEREAEARNYGKFVIAPLERGFGDTLGNSLRRV
                     LLSSLDGAAITSMRISDVTHEFVDIPGVREDVIQVILQIKQIRMILHEGDTSHLHLDV
                     RGEGVVTAADLQVPAEVEIVNPELYLFTVDDAKTKLDFDFTVERGRGYSPATDRSGKL
                     PIGELPVDAIYSPVKRVNFTVGSARVGQSTNYDRLNLEIWTDGTISPEKALASSSKVI
                     IDHLRLISGVSEESILLGAEKEMTGSHLTSEAAETPIENLDLTVRVFNSLKRTGVRSV
                     GDVLELLEKGEEAVMSIRNFGEKSLDELREKMKEKGYLPDEKELE"
     gene            complement(2287..2925)
                     /gene="rpsD"
                     /locus_tag="PLW25_05075"
     CDS             complement(2287..2925)
                     /gene="rpsD"
                     /locus_tag="PLW25_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOS53896.1"
                     /translation="MAKYTGPACKLCRREGEKLFLKGSRCFSPKCSFEKRGFIPGQHG
                     RTRQGRSERESDYARQLRAKQRARRVYGMMERPFRRYYQVALRTRGLPGLNLLRTLET
                     RLDNVVYRLGYAENRAQARQLVNHGHFEINGRRADIPSMLVKPGDTVTVKETSLKLEH
                     FKALADLAEKRLCPVWLDRDVKKLSGRVLRLPERSEIEGSLNEQLIVEYYSR"
     gene            complement(2947..3351)
                     /gene="rpsK"
                     /locus_tag="PLW25_05080"
     CDS             complement(2947..3351)
                     /gene="rpsK"
                     /locus_tag="PLW25_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOS53897.1"
                     /translation="MAQNVRSTRRTSTTRKVKRTLSSAQVHIFASFNNTIVTVTDQQG
                     NSLCWGSAGSAGFKGSRKSTPFAARLAAEQAIKAAQNMGVQEVDMIIKGPGPGRESAI
                     RAIQSLGLKVRSITDITPVPHNGCRPPKKRRV"
     gene            complement(3458..3847)
                     /gene="rpsM"
                     /locus_tag="PLW25_05085"
     CDS             complement(3458..3847)
                     /gene="rpsM"
                     /locus_tag="PLW25_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOS53898.1"
                     /translation="MARIEGIDLPRNKRIEVGLTSIFGIGPTRAREILRSTQVNPDTR
                     VKDLTENEVAALREFISKNYKVEGDLRREVQMNIKRLIEIGCYRGLRHRRNLPAHGQR
                     TRTNARTAKGPKKTVAGRGRRRGAAKK"
     gene            complement(3856..3969)
                     /gene="rpmJ"
                     /locus_tag="PLW25_05090"
     CDS             complement(3856..3969)
                     /gene="rpmJ"
                     /locus_tag="PLW25_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOS53899.1"
                     /translation="MKVTTSIRKRCAKCKIVKRSGVLFVICENPKHKQRQG"
     gene            complement(4019..4822)
                     /gene="map"
                     /locus_tag="PLW25_05095"
     CDS             complement(4019..4822)
                     /gene="map"
                     /locus_tag="PLW25_05095"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOS53900.1"
                     /translation="MSWDRQVTIKTPQEIALMREAGRINAEALAAARALVRPGVTTAD
                     LNAAAEEVLRKYGCYSPFKGYPGPYPYPASTNVSVNHELVHGIPGKRRLKEGDIVSVD
                     CGTVYEGYVGDAAFTEMVGEVSDEARRLVAATEGALFAGIAQMVAGNHLGDVSAAIQA
                     YAEERGFHVTREYTGHGVGRAMHEGPQAPNYGTPGRGMVLRAGMTIALEPMLLVGTPY
                     TKVLPDQWTVSSADGSLTAHFEHTIAVTENGPLILTTQGDGSILDGKWK"
     gene            complement(4823..5479)
                     /locus_tag="PLW25_05100"
     CDS             complement(4823..5479)
                     /locus_tag="PLW25_05100"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOS53901.1"
                     /translation="MAVYIVLLGPPGAGKGTQAQLIAQKYGLMHVSSGDLFRENLKNE
                     TELGKLAQTFMQRGELVPDDVTIAMVRERLGRPDCQKGALLDGFPRTPTQAQALAQML
                     QEFNAAVSVVPYIAVPEEVLVERLSGRWTCRKNGHVFHEKYNPPTAVGVCDYDGSELY
                     QRDDDLPATVKNRIKVYQEQTAPLIQYYRENGLLVEVDGTKPIDQVTNALLQAIEKAA
                     "
     gene            complement(5481..6812)
                     /gene="secY"
                     /locus_tag="PLW25_05105"
     CDS             complement(5481..6812)
                     /gene="secY"
                     /locus_tag="PLW25_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOS53902.1"
                     /translation="MKRSAWRYLWTSEDIRKKLLITVLLLVIYRLAANIPVPGINREV
                     IRSISEMGGPGASLFNLLDLLSGGTISNFSILAMGVYPYITSQIIIQLLVPIIPALDR
                     RLKDNPREGRSLMEKWTTMLTIPMAFLSAIGQVNIFNSMAKTGSVLTTPFGFVGASLL
                     PTITLLLSMVAGTMFGIWLGQLISEFGIPNQGLSLIIFAGIISKIPSNLLGILADKEN
                     GWWVLIIILVLLALTIFAIVYVQQGRRNVPVLFPGRRIGNRMSMPVRSNLPLMVNMAG
                     MIPLIFAQTFLSFPALIASYFIAGKTSGFWYGVYSLFSGSGWFYPIIFFVLVVAFTFF
                     YTDVLFTQQNYGDTLKKQGAQIPGVVRGGPTQKYLTRVQRRITFPGAFFLGFVAVLPY
                     IIGTILPITRASAFVISAAGLLIVVGVVRDTFTIIETELKVHGYDESIIKG"
     gene            complement(6835..7287)
                     /gene="rplO"
                     /locus_tag="PLW25_05110"
     CDS             complement(6835..7287)
                     /gene="rplO"
                     /locus_tag="PLW25_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOS53903.1"
                     /translation="MKLHDLMPNEGAKKAPKRKGHGISAGQGKTAGRGTKGLGARAGS
                     GGHLYRQGGNLPFYRRLPFIRGEGFTPPNRVEFNEVNLDQLERFQAGSIVSRETLQEN
                     GLLHKASNPVALLGRGEVKVALTVQVQRVSKSAREKIEAAGGKVEIVA"
     gene            complement(7284..7493)
                     /gene="rpmD"
                     /locus_tag="PLW25_05115"
     CDS             complement(7284..7493)
                     /gene="rpmD"
                     /locus_tag="PLW25_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006640333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOS53904.1"
                     /translation="MPKKKEALKKLRITLVKSAIGYSETHKATIRALGLRRMNQTVEQ
                     VDSPVIRGMLMKVNHLVKIEEQEVK"
     gene            complement(7486..8007)
                     /gene="rpsE"
                     /locus_tag="PLW25_05120"
     CDS             complement(7486..8007)
                     /gene="rpsE"
                     /locus_tag="PLW25_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOS53905.1"
                     /translation="MDYQPQETQFDERVIEIARVAKVVKGGRRFQFRVTVVVGDNNGK
                     IGIGIGKANAVPDAMRKATERAHKNIRKVTLTGTTIPHEVTGRTAGAVVLLKPASAGT
                     GVIAAGGVRAVLEAVGVHDILTKSLGSANVLNVVKATFDALAQLKSPETEAARRGKPV
                     KELMPYWERRKNA"
     gene            complement(8047..8412)
                     /gene="rplR"
                     /locus_tag="PLW25_05125"
     CDS             complement(8047..8412)
                     /gene="rplR"
                     /locus_tag="PLW25_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOS53906.1"
                     /translation="MAKNNRTEARIHRHRRVRKYISGTESRPRLSVFRSLAEIYAQVI
                     DDQAGVTLAAASSIDHELRGKTDGKKKLDQAKLVGELLARRAKDKGITRVVFDRGGFR
                     FSGRVKALADAAREAGLEF"
     gene            complement(8428..8976)
                     /gene="rplF"
                     /locus_tag="PLW25_05130"
     CDS             complement(8428..8976)
                     /gene="rplF"
                     /locus_tag="PLW25_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOS53907.1"
                     /translation="MSRIGKLPIRIPDSVQVEINGSEVTVKGPKGEMKRQFSPDIIIT
                     QEDGMLLVKRNSDLAPVRALHGTTRALLFNMVTGVSTGFTTELEVDGVGYRVEMEGEK
                     LVLFVGYSHPFKIDPPKGVHFEIDSKTHLVKINGSDKEVIGQLANDIIKIRPPEPYLG
                     KGIRYKGQKIRRKAGKAGKAKA"
     gene            complement(9004..9417)
                     /gene="rpsH"
                     /locus_tag="PLW25_05135"
     CDS             complement(9004..9417)
                     /gene="rpsH"
                     /locus_tag="PLW25_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOS53908.1"
                     /translation="MSVNDPIADMLTRIRNGVIGSQSVVAMPHSKVKAEIARILKEEG
                     FIEGYEVVEGDKPGMMTLRIKIKYVGERREKTPVITGLERISRPGRRVYSKKQDIPWV
                     LAGMGIAILSTPKGVMTGQRARQLGIGGEVLCKVW"
     gene            complement(9439..9621)
                     /locus_tag="PLW25_05140"
     CDS             complement(9439..9621)
                     /locus_tag="PLW25_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOS53909.1"
                     /translation="MAKKCMEYRELRREYPNQVRNRCKRCGRPRGYLRRFGLCRICFR
                     ELALEGEIPGVTKSSW"
     gene            complement(9623..10156)
                     /gene="rplE"
                     /locus_tag="PLW25_05145"
     CDS             complement(9623..10156)
                     /gene="rplE"
                     /locus_tag="PLW25_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOS53910.1"
                     /translation="MKERYIKEVSPALMKSLGLSNVMQIPRIKKVVINIGMGEAMENP
                     KALDAAVQDLSAITGQKPVITKARKSIANFKLREGRAIGTAVTLRGEKMWAFLDRLMN
                     IVLPRVRDFRGVSAESFDGRGNYTLGLREQIIFPEIEYDKVDKVRGMEITIVTTAPSD
                     DQAAALLQLLGMPFRKE"
     gene            complement(10187..10519)
                     /gene="rplX"
                     /locus_tag="PLW25_05150"
     CDS             complement(10187..10519)
                     /gene="rplX"
                     /locus_tag="PLW25_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOS53911.1"
                     /translation="MKVKIRKGDQVEVISGTINDKGKRGEVIKVLPDENRVVIQGVNT
                     RTKHQRQVQSQGRTVNPGLVRFEAPISISNVAIVCKKCNKSTRVGLVREGDKTKRQCK
                     ECGALIDE"
     gene            complement(10542..10913)
                     /gene="rplN"
                     /locus_tag="PLW25_05155"
     CDS             complement(10542..10913)
                     /gene="rplN"
                     /locus_tag="PLW25_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOS53912.1"
                     /translation="MIQQETRVKVADNTGARELLVIQVVGGSRRKYGAVGDVVIATVK
                     SAAPQGSVKKSEIVKAVIVRASKEWRREDGSFIRFDDNAAVILDVDGQNPKGTRIFGP
                     VARELRERGYMKIISLAPEVL"
     gene            complement(10910..11188)
                     /gene="rpsQ"
                     /locus_tag="PLW25_05160"
     CDS             complement(10910..11188)
                     /gene="rpsQ"
                     /locus_tag="PLW25_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOS53913.1"
                     /translation="MNQRRRLTGIVTSNRMMKTVVVEIARTYKHPLYHKVVHARQTIK
                     AHDELGCAIGDKVMVVECAPMSKEKTWMVQSILKREGKTLDVAEEAVE"
     gene            complement(11185..11397)
                     /gene="rpmC"
                     /locus_tag="PLW25_05165"
     CDS             complement(11185..11397)
                     /gene="rpmC"
                     /locus_tag="PLW25_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOS53914.1"
                     /translation="MKTSEIRLLSTEEIKTKLADTRNELMNFRFQLVTGQLTDTSRLK
                     TTRRQIALYETILREREQGKKLEGAK"
     gene            complement(11394..11822)
                     /gene="rplP"
                     /locus_tag="PLW25_05170"
     CDS             complement(11394..11822)
                     /gene="rplP"
                     /locus_tag="PLW25_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOS53915.1"
                     /translation="MLMPKRVKWRKVMRGRMTGKAQRGADMIVGEYALQAVEPGWVTA
                     RQIEAARRTIVHSMKRHGKVWIRIFPDKPYTHRPPETRMGKGKGNVEYYVAVVKPGRI
                     MFEISGLEREEAIHALNQAGYKFSIKTKVLARGQEAGEGV"
     gene            complement(11870..12547)
                     /gene="rpsC"
                     /locus_tag="PLW25_05175"
     CDS             complement(11870..12547)
                     /gene="rpsC"
                     /locus_tag="PLW25_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOS53916.1"
                     /translation="MGRKVHPIGFRLGINQPWEGRWYAEGSDYRTQLHQDLVIRRMVQ
                     SQGEKAGISLVEIERFPGKVKVVVHTAKPGILIGRKGDSVKKIRTDLEALTGKAIDLE
                     IKEIKAPDCDAFLVAKNIAGQIERRVSYRRAMKRALQQAMRQGAEGIKVSVSGRLSGA
                     EMARIVWLREGRVPLQTLRADIDFARAEALTTYGRIGVKVWVYKGEVIPGVEEKVEPT
                     EGVYVSE"
     gene            complement(12608..12949)
                     /gene="rplV"
                     /locus_tag="PLW25_05180"
     CDS             complement(12608..12949)
                     /gene="rplV"
                     /locus_tag="PLW25_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOS53917.1"
                     /translation="MATDIRAELKNCGVSAQKTRLVIDLIRGKPAVEALTTLKFTNKA
                     AARHVGRLLASAVANAEENFGVNRDDLVVYKISADDAPLRKWRRFGARGRFKPLLRRS
                     SHITVVLREKE"
     gene            complement(12952..13230)
                     /gene="rpsS"
                     /locus_tag="PLW25_05185"
     CDS             complement(12952..13230)
                     /gene="rpsS"
                     /locus_tag="PLW25_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOS53918.1"
                     /translation="MSRSLKKGPFVDPKLLKKIEAMNTRSEKKVVRTWSRASTIFPQM
                     VGHTIAVHDGRRHVPIYITENMVGHRLGEFAPTRHFRGHMSDKSTGAR"
     gene            complement(13271..14107)
                     /gene="rplB"
                     /locus_tag="PLW25_05190"
     CDS             complement(13271..14107)
                     /gene="rplB"
                     /locus_tag="PLW25_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOS53919.1"
                     /translation="MAIKVYKPTTPSRRAMTGYSFEEITKDTPERALIVIRKARAGRN
                     NHGKVTVRHQGGGSKQYIRIIDFKRDKREIPARVAAIEYDPNRTARLALLTYVDGEKR
                     YIIAPVGLKVNDTVVAGPMAEVRPGNSLPISNIPVGTLVHNIELKEGKGGQLVRSAGA
                     SAQLLAKEGDFAQIRMPSGEVRLVPQKCYATIGQVGNTDHSNIKLGKAGRKRHMGIKP
                     TVRGTAMSPRDHPHGGGEGRQPVGMSGPKSPWGKPTRGYRTRRNKKTDQYIVRRRNAG
                     NR"
     gene            complement(14107..14409)
                     /gene="rplW"
                     /locus_tag="PLW25_05195"
     CDS             complement(14107..14409)
                     /gene="rplW"
                     /locus_tag="PLW25_05195"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOS53920.1"
                     /translation="MSTIYDVLRRPIVTEKTNYMVTKLHQYVFEVADDANRTMVKDAV
                     EKLFNVNVLRVNIINAPAKRSRRARSRRLLIRNAEYKKAVVTLSPEDRIPIFEGVE"
     gene            complement(14411..15049)
                     /gene="rplD"
                     /locus_tag="PLW25_05200"
     CDS             complement(14411..15049)
                     /gene="rplD"
                     /locus_tag="PLW25_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOS53921.1"
                     /translation="MEVEVLNMEGKKVSKVELPPAIFEAPINIDLMHQAYVMQMANAR
                     LGTHETKVKSEVSGGGRKPWRQKGTGRARQGSTRSAQWKGGGKIHTPHMRSYVQNMPK
                     KMRQAALRSALSVKAAESSIVVVDALKLKEPKTRLMASALNTLVGDAKALVLIPSAES
                     YEDVLRSTNNIPDAKVLNANYINVRDIMVFDKLIMPVAALELITANLGGVGE"
     gene            complement(15097..15726)
                     /gene="rplC"
                     /locus_tag="PLW25_05205"
     CDS             complement(15097..15726)
                     /gene="rplC"
                     /locus_tag="PLW25_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOS53922.1"
                     /translation="MFKGLIGKKIGMTQIFDENGAAHPVTLIEAGPCFVTQVRTVQKE
                     GYSAVQLGYEEVKPRRLASGELGHLRRNSLPPLKYLREFRAKEVQVAEGDKVDVNLFA
                     VGEKVDVSGISKGKGFAGGVKRYHFRGGPKSHGQSDRMRAPGSRSSTTTPGRVFKGAR
                     GAGHMGNDAVTAQNLKVVLVDAERNVIGVNGSVPGARGGLVLIKESRKG"
     gene            complement(15988..16296)
                     /gene="rpsJ"
                     /locus_tag="PLW25_05210"
     CDS             complement(15988..16296)
                     /gene="rpsJ"
                     /locus_tag="PLW25_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOS53923.1"
                     /translation="MAKQKIRIRLKAYDHRVLDQSAKRIVETAERSGAQVVGPVPLPT
                     RIERFTVRRSSFIDKDSHEHFEIRTHNRLIDVLDPDAKTIDMLMRLNMPAGVDIEIKI
                     "
BASE COUNT         3523 a         4632 c         3994 g         4218 t
ORIGIN      
        1 agcaaccatt caggttcctg ttttcctttg aggacgtaag ttttgtccac tttctcatct
       61 ccattggcgg ggtatatccc tcgccattga ttaatttttt tcatcctcgc aacgctcacg
      121 cgtaagacct gcataactca catttaccag aaagagcccc tgggctggtg ccaggcccgg
      181 ttttaacggg cggccttctc gtaatgctgt ttcaatctca ctttcatcag ccccgctggc
      241 ggcttgcacc tgtataaaga ccatccggcg gaccatgtga taaagaaacg cgttcgcttc
      301 gacttcgtac cgccaacctt ctggggtttt catccattgg ctggtataca cttcgcgtgt
      361 cgtgctgccc cctttcttca tggctctgcc aaaagcgcaa aaatcatgcc tgccaatgaa
      421 cctgcttgct gcccggtcga gcctttccgg gtccaattca ggccagatgc gccaggcgta
      481 acgctccaaa aggggctgcc tcacgggtaa ctggtaaagg tgataatggt acgtgcggct
      541 tgaagcgtca taacggggat ggaattccgc ttgagccaca ttggcttcga tcaccgaaac
      601 atcatcgggt aagcgggcgt tcaacgcatt taccagatca gcagatgagt gttgccaatc
      661 cagtgagatt gtcgccacct gtccacaggc gtgcacacct gcatcggtgc ggcctgcgaa
      721 aagcatggcg ggttgctgcc agcccagctc cctaagaaca gtttcaacca ccaactggac
      781 agttcgtgtt tttccctggc gttgaaaacc tgagaaatca gtaccgtcgt aggcgaggat
      841 aagtttgtaa agtgccatgc caatccatcc ggaaacccgg aaggattatt cttctaccag
      901 ctcgatctgg accatcatgg cggaatcgcc aaggcggggt ccaagtttgt agattcgggt
      961 ataaccgcct ttacggtttt cgtaccgcgg ggcgatttca tcaaacaact ttctgaccat
     1021 ggctgcggaa cccaatcggg cggcggccag cctgcgcgcg tgaacttttt cagcatccgt
     1081 accgctgttg ctgcggcgcg cgagcgtgat taattgttct gcttctccac gtataaataa
     1141 agccttggtg tgggtggttt gaatccgttc gtgttcgaac agttgtttca ccatcgtgcg
     1201 gcgcaaacca gcgcgatggt ctttttgacg tcctaatttt gaacctgcaa tctgatggcg
     1261 catctttacc tactccaact ctttttcatc aggcaaatag cctttttctt tcatcttttc
     1321 acggagctca tccaggcttt tctcgccaaa gttccggatt gacataacag cctcttcgcc
     1381 cttttccaat aattccagca catcgcccac cgatctcacg ccagtcctct tgagcgaatt
     1441 gaacactcgc acggtgagat ccaggttttc aatcggagtc tcagcagcct cgcttgtgag
     1501 atggctgcct gtcatttctt tttcagctcc gagcagaatg ctctcttcgc tcacgccgga
     1561 aatcaacctt aaatgatcaa tgatgacctt tgacgaagaa gccagcgctt tttcaggtga
     1621 aattgtgccg tccgtccaaa tttcaagatt aagacggtca taattcgtgc tctgaccaac
     1681 gcgagcggag cccaccgtga agttgacgcg tttaaccggg ctgtagatgg cgtctaccgg
     1741 caattcgcca atgggcagtt ttccactgcg atcggtcgcg ggagaataac cgcgtccccg
     1801 ctccacagtg aagtcaaaat ccagcttcgt cttggcatcg tccaccgtaa agagatacaa
     1861 ctcggggtta acgatttcaa cttcagccgg cacctgtaaa tcagcagccg ttaccactcc
     1921 ctcgccccgc acatccaggt gcaaatggga ggtatcgccc tcgtggagga tcatacgaat
     1981 ctgtttgatc tgcaagatca cctggataac atcctcccga acaccgggga tatcaacaaa
     2041 ttcgtgcgtc acatcagaaa tacgcataga ggtaatcgcc gcgccatcca gtgatgaaag
     2101 cagcacacgc cgtaatgagt tacccagcgt atcgccaaaa cccctctcca aaggagcgat
     2161 gacgaatttc ccgtaattgc gggcttctgc ctcgcgttca atcttgggtg tgaccatatt
     2221 gattaaagcg cacataatac accctttccg atactcactt gtatcctttc atccttttag
     2281 cccgctttaa cgtgagtagt actcaacaat caactgttcg ttcaggctgc cctcgatctc
     2341 agagcgttct ggtagcctga gcactcgacc ggaaagtttt ttcacatccc tgtccaacca
     2401 gacggggcat aatcgttttt cagccaaatc agcaagtgct ttgaagtgtt cgagctttaa
     2461 tgaggtctct tttacggtaa cggtatcacc tggttttacc agcatcgagg ggatatctgc
     2521 gcgccgccca ttgatctcaa aatgaccgtg gtttacgagc tgccgggcct gagcgcgatt
     2581 ctccgcgtac cccagtcggt ataccacgtt atccaggcgt gtttcgagcg tgcgcagcag
     2641 gtttaaaccc gggagaccgc gcgtgcgcag tgcgacctgg tagtaacgcc tgaaagggcg
     2701 ttccatcata ccgtatacac ggcgggcgcg ttgtttggcg cgcaattggc gggcgtaatc
     2761 cgactcacgc tcagagcgac cctgtcgggt gcgtccgtgc tggccgggaa taaaaccccg
     2821 tttttcgaaa gaacacttgg gcgagaaaca acggctgccc ttcaagaata atttttcacc
     2881 ttcacgccgg caaagtttac aagccggacc agtatatttg gccatatctt aaccttctcc
     2941 ttttaattac acgcggcgtt ttttcggagg ccggcagcca ttatgcggta ccggtgtgat
     3001 atccgtaatc gagcgcactt ttaaacccag ggattgaata gcgcgaatgg cggattccct
     3061 gccgggtccc ggccctttga tgatcatgtc cacttcctgg acacccatat tttgcgcagc
     3121 cttaatcgcc tgttcagccg caagacgggc ggcgaagggt gtgctcttgc gtgagccttt
     3181 gaatccggct gaacctgcgc ttccccagca gagcgagttg ccctgttgat cagtcaccgt
     3241 cacaatcgtg ttgttgaaag acgcgaaaat gtgcacctgg gctgatgaca gggttcgttt
     3301 cactttgcgg gtcgtgcttg tgcgacgcgt tgaacgaaca ttttgagcca tattacctcg
     3361 tttacctttt tctaaatcac gttagtttct actaaagagc ctgaccgggc tgccgcgggg
     3421 ggagaaggca ccccacccgg actggttcta aagcagatta cttcttcgct gctccacggc
     3481 ggcgtccgcg accggcaaca gtcttcttag gacccttagc ggtacgggcg ttggtgcgtg
     3541 tgcgttgccc atgagccggc aggttacggc ggtgccgcaa accgcggtaa caaccaattt
     3601 caataagccg cttgatgttc atctgcactt cgcgccgcag gtcaccttcg actttatagt
     3661 tcttcgaaat aaactctcga agagccgcaa cttcattttc agtcaggtct ttaacccggg
     3721 tatcagggtt aacctgagta gaacgcaata tttcacgtgc tctggtagga ccaatgccaa
     3781 aaatgctggt cagaccaact tcgatccgct tgtttcgcgg tagatcaatg ccttcaattc
     3841 tcgccataag ttcctctacc cttgtcgctg cttgtgctta ggattttcgc aaataacaaa
     3901 cagaacgccg ctgcgtttca cgattttaca tttcgcacaa cgtttgcgaa tggatgttgt
     3961 taccttcata atatctgttc ctcgctctcg ttcttaatcc gaattttcaa ttttactact
     4021 acttccattt tccgtccaga atcgacccgt ctccctgcgt agtgaggatc aaaggtccat
     4081 tctccgttac cgcaattgta tgttcgaagt gcgcggtcag cgaaccatct gctgaagata
     4141 ccgtccactg gtcaggcagt accttggtgt aaggtgtgcc aaccagcaac atgggttcca
     4201 gcgcgatggt catgccggcg cgcagtacca tccctctgcc cggggtgcca taattgggcg
     4261 cttgagggcc ttcgtgcatc gctcttccca cgccgtgccc ggtatattca cgggtaacgt
     4321 ggaatccgcg ctcttcggcg tatgcctgta tggctgccga gacatccccc aggtgatttc
     4381 ctgccaccat ttgggcaatg ccggcgaaca gcgctccttc ggtggcagcc accaggcggc
     4441 gtgcttcatc gctcacctcg ccaaccatct cagtgaacgc cgcgtctcca acataccctt
     4501 catagacagt gccgcaatcc accgaaacga tatccccttc tttcaacctg cgtttgcctg
     4561 gaataccgtg caccaattca tggttgacgc tcacattggt gctcgcgggg taaggataag
     4621 gaccagggta acctttaaaa gggctgtaac agccgtattt acgcagcact tcttcagctg
     4681 cagcgttgag gtcagcggtg gtcaccccag gacgcaccaa cgcgcgcgca gcagccagcg
     4741 cttcagcatt gattctgcct gcttcccgca tcagggcgat ttcctgcggc gttttaatgg
     4801 ttacctgtcg gtcccaagac atttacgcgg ctttctcgat cgcttgtaaa agagcattcg
     4861 ttacctggtc gatgggcttg gtgccatcca cctcgaccag cagtccattt tcacggtaat
     4921 attgaatcag cggggcggtc tgttcctggt aaaccttgat gcggtttttg acagtggctg
     4981 gcaagtcgtc atcccgctgg taaagctcag aaccatcata gtcgcacacg ccgaccgctg
     5041 ttggtggatt atatttttca tggaatacat gcccgttctt acggcaggtc caccgaccgc
     5101 ttaatcgttc caccagtacc tcttcgggaa cagcgatata cggaactaca ctgacggctg
     5161 cgttgaactc ctgcagcatt tgcgccagcg cctgagcctg tgtcggagtg cgtggaaaac
     5221 cgtctaagag cgcgcctttt tgacaatctg ggcggccgag ccgttcgcgt accatggcaa
     5281 tcgtgacgtc atcaggaacc aattcaccac gctgcatgaa cgtctgggcg agttttccca
     5341 gctctgtttc gttcttcaaa ttctccctga agagatcgcc agaagaaaca tgcatcaacc
     5401 catatttttg cgcgatgagt tgggcttgcg ttccttttcc agcgcctggt ggtccaagaa
     5461 gaacaatgta cacagccata ttaacctttg ataatacttt cgtcgtaacc atgaactttg
     5521 agttcggttt cgatgatggt gaaggtatca cgcacaacgc cgactacaat caacaggcct
     5581 gcggctgaaa ttacaaaggc gctcgccctg gtaattggca ggatcgtgcc gatgatatag
     5641 ggtaaaacag ccacaaaccc gaggaagaat gcgcccggga aagttatgcg gcgctgcacc
     5701 ctggtgaggt atttttgcgt tggtccacca cgaaccaccc ccggaatttg cgcaccctgc
     5761 tttttgagcg tatcaccgta attctgttgg gtgaacaaca catctgtgta gaagaaggtg
     5821 aacgccacga ccaggacaaa gaaaatgatc gggtagaacc agccactacc gctgaacagg
     5881 gagtaaacgc cgtaccaaaa accggaagtt ttaccggcga tgaagtaact ggcgatcagc
     5941 gccgggaacg acaggaaggt ttgcgcgaag ataagcggga tcatacctgc catgttgacc
     6001 atgagcggta aattgcttct cacgggcatc gacatgcgat tgccaatgcg ccttccaggg
     6061 aacagcacgg gtacattgcg tctgccctgc tgcacgtaaa cgatggcgaa aattgtgagc
     6121 gccagcagga ccaggatgat gatcagcacc caccagccat tttctttatc tgccaggata
     6181 cccaacaggt tggaaggaat ttttgaaatg ataccggcga agatgatcaa cgataaaccc
     6241 tggttgggaa taccaaattc agaaatgagt tgacccagcc agataccaaa catcgtgccc
     6301 gccaccatgc tcaatagcag cgtaatcgtc ggtaacaatg aagcgccgac aaacccaaaa
     6361 ggcgtggtca gcacgctgcc ggtttttgcc atcgaattga agatgttgac ctgcccaatg
     6421 gctgaaagaa acgccatggg aatggtcagc atggtcgtcc atttttccat caaagaccgg
     6481 ccttcgcgcg ggttgtcttt cagcctgcgg tcaagggcag ggatgatggg caccagcaac
     6541 tggataataa tttgcgaggt gatgtacggg tatacaccca tcgcaaggat ggaaaagttc
     6601 gaaatagttc cgcctgacag caggtcgagc aggttaaaaa gcgatgctcc cggtccaccc
     6661 atctctgaaa ttgagcggat gacctcgcgg ttgataccgg gaacagggat attcgctgcc
     6721 agccggtaga tcaccagcaa gagcacagta atcaacagct tcttgcggat gtcttcagat
     6781 gtccaaaggt aacgccaggc agatcgcttc attcaacact ccttaggatg gcgttcaggc
     6841 gacaatctct actttgccgc cagcggcttc gatcttctca cgagctgatt ttgaaactcg
     6901 ttgcacctgc acggttagcg caaccttaac ctcgccgcgg ccaagcaggg ccacagggtt
     6961 gctggctttg tggagcaacc cgttttcctg cagggtttcg cgagagacga tgctgcccgc
     7021 ctggaagcgc tctaattgat caagattgac ctcgttgaat tcaacccggt tgggaggggt
     7081 aaagccctca ccacggatga aagggaggcg gcgataaaaa ggcaggttac caccctgacg
     7141 ataaaggtgc cccccagatc ctgcacgagc gccaagaccc ttggtgccgc gtccggcggt
     7201 ctttccctgc cccgcagaga taccgtgacc ttttcgtttc ggggcttttt tcgccccttc
     7261 gtttggcatt agatcgtgta atttcatttg acttcctgct cttcgatttt tacaagatga
     7321 ttaactttca tcagcatgcc gcgaatcacc ggtgaatcaa cctgttcgac agtttgattc
     7381 atgcggcgca gacccagcgc gcggatggtg gctttatgcg tttcggaata accgatggcg
     7441 cttttcacca acgtgatgcg caattttttc agcgcttctt ttttcttagg cattcttcct
     7501 ccgttcccag taaggcatca gttctttcac gggttttccg cggcgggctg cctcggtttc
     7561 gggggatttc aattgcgcga gcgcgtcgaa agtggctttc accacgttca atacgtttgc
     7621 gctgcctaat gacttggtca gaatatcgtg cacgccaact gcttcaagta ccgcgcgcac
     7681 gccgccagca gcgatcacac cagtaccagc tgaggcaggt ttgagcagta caaccgcccc
     7741 ggctgtgcgg cccgtaactt catgcgggat ggtcgtgccg gtgagcgtga ccttgcggat
     7801 gttcttgtgg gcgcgttcgg tggctttgcg catcgcgtct ggcacggcgt tcgccttgcc
     7861 aataccgata ccaattttgc cgttattatc tccgacaaca acagtgaccc tgaactggaa
     7921 gcgccggccg cctttaacca cttttgcgac tctggcaatc tcgattaccc gttcgtcaaa
     7981 ctgcgtttcc tggggttgat aatccatcat atccataaaa tgagccctct atctcttcct
     8041 tctaccctaa aactcaagac cggcttcacg agccgcgtct gccagcgctt tcacacggcc
     8101 gctgaatcga aaaccgccgc ggtcaaacac aacccgggta atgcctttgt cttttgcgcg
     8161 ccgggcgagc agctcgccta ccagtttcgc ctggtccagc tttttctttc cgtccgtttt
     8221 gccgcgcaat tcatggtcga tgcttgaggc ggcggcaagt gtgacgccgg cctgatcatc
     8281 gattacctgg gcgtaaattt cagccaggct gcgaaatacg ctgaggcgag gtcgggattc
     8341 agtaccgctg atgtacttgc gcacgcggcg gtgtctgtga attcttgctt ctgtacgatt
     8401 attttttgcc atattccctc acctgcttta tgccttcgcc ttgccggctt taccggcttt
     8461 gcggcgtatc ttttgacctt tgtaacgtat acccttgccc agatagggtt cgggtggtct
     8521 gatcttgata atgtcattcg ccagttggcc gatgacctct ttatctgacc cattgatctt
     8581 caccaggtgg gtcttgctgt cgatctcaaa gtgcacgcct tttggcgggt caattttgaa
     8641 gggatgcgaa taaccaacaa acaataccag tttttcgcct tccatttcca cgcgatatcc
     8701 cactccatcc acttccaatt cagtggtgaa accggtagac acaccggtga ccatgttaaa
     8761 gagaagggcg cgcgtggtac cgtgcaatgc cctcactggc gcgaggtcag aattgcgttt
     8821 gaccaacagc atcccatcct cctgggtgat gatgatatca ggagaaaact ggcgcttcat
     8881 ttcacccttc ggtcctttta ctgttacctc agaaccgtta atttcaacct ggactgaatc
     8941 aggtattctg atgggtaatt tacctatacg agacacgata tctatcctcc ttcatcccag
     9001 gggctaccaa accttgcaaa gaacttcgcc accaattccg agctggcgag ctctttgtcc
     9061 ggtcattaca ccctttgggg tggataggat tgcgataccc atccctgcca aaacccaggg
     9121 gatgtcttgt tttttactgt agacacggcg gccagggcgg ctgatgcgct caagaccggt
     9181 gataacgggt gttttttcac gccgttcacc cacatacttg atcttgatgc gcaaagtcat
     9241 catccccggt ttgtcgcctt ccaccacttc atatccttca atgaaacctt cctccttcaa
     9301 gatgcgggcg atttccgctt tgaccttgga gtgaggcatg gcaaccactg attggctgcc
     9361 aatgacgcca ttccggatac gggtcaacat atcagcaatt ggatcattta cactcatgtt
     9421 tcttcctccg aattggcatt accaggatga cttggtcacg ccgggaattt cgccttcgag
     9481 agccagctct ctgaagcaaa tgcggcataa accaaagcgc cgcaggtaac cgcgcggtct
     9541 accgcagcgt ttacagcggt tgcgcacctg attgggatat tcccgtctta attcacgata
     9601 ttccatgcat ttctttgcca tactattcct tcctgaaagg catgccaagc agttgcagca
     9661 gggctgcggc ttgatcatca gatggtgcgg tagttacgat ggtgatttcc ataccacgta
     9721 ctttatcgac tttgtcatac tcgatctcag ggaatatgat ttgctccctt aaacccagtg
     9781 tgtagttacc tctgccgtca aaagactccg ccgacacgcc gcggaaatca cgcacacggg
     9841 gcaagacaat gttcatcaat cgatccagaa aagcccacat cttttctccc ctcagcgtga
     9901 ccgcagtacc aattgcgcgc ccttcgcgca gtttgaagtt cgcgatgctc ttgcgcgcct
     9961 tggtgatcac tggtttttga ccggtgatgg cgctcaggtc ctgcactgcc gcatccaacg
    10021 ctttgggatt ttccatcgcc tcgcccatgc cgatattgat gaccactttc ttaatcctcg
    10081 gtatctgcat cacattggat aagccgagtg atttcatcaa cgccggtgag acttctttaa
    10141 tataacgttc tttcattaaa ttcattagaa ccctctctca atcggcttat tcatcaatca
    10201 gtgcgccgca ttccttgcat tgacgctttg ttttatcgcc ttcgcgtacc aggccaacgc
    10261 gcgtagattt attacatttt ttgcacacga ttgccacgtt ggaaatgctg ataggggctt
    10321 caaaacggac gaggcccggg ttgactgtgc gaccctgtga ttggacctgg cgctgatgct
    10381 tggtgcgtgt gttcacaccc tgaataacca cgcggttctc atccggcagc actttgatca
    10441 cttcgccgcg cttgccctta tcattgatgg tgccgctgat tacttccacc tgatcaccct
    10501 tacgaatttt tactttcata ataattgctc ctatccctct gttagagtac ttctggcgcc
    10561 agagagatga tcttcatata gccccgttca cgcagttcgc gcgcgacagg accgaagatg
    10621 cgtgtaccct tcgggttctg accatccaca tcaaggatca ccgcggcgtt gtcatcaaag
    10681 cggatgaatg agccatcttc gcggcgccac tctttggagg cgcgaacgat aaccgctttg
    10741 acgatttcgc tctttttcac cgacccctga ggggctgctg acttgacagt ggcaataacc
    10801 acatctccaa ccgcaccata tttccgcctg gaaccgccca ccacctggat gacaagaagt
    10861 tcccgcgcgc cggtgttgtc ggcgaccttt acacgagtct cctgttgaat cattcaaccg
    10921 cctcctcagc cacatcaagc gttttccctt cgcgcttgag gatggactgc accatccagg
    10981 tcttttcttt cgacatcggg gcacattcca ccaccatcac tttatcgcca atcgcgcaac
    11041 ccagttcatc atgcgctttg attgtttgcc gggcgtgcac cactttgtga tatagcgggt
    11101 gtttgtacgt gcgagcaatt tcgacaacca ctgtcttcat catccggttg cttgtgacga
    11161 tccctgtcaa tctacgtctt tggttcattt tgcaccttcc agtttcttgc cttgctcgcg
    11221 ttcgcgtaaa atcgtttcgt acaacgcaat ttgccgccgg gtggttttca atcgactggt
    11281 atcggtcaat tgaccggtga ccagctgaaa gcggaagttc attaactcgt tgcgcgtatc
    11341 agcgagtttg gtcttgatct cttctgtgga taacaagcga atctcagagg ttttcataca
    11401 ccctccccgg cttcctgccc acgggccagc actttggtct tgattgaaaa tttataaccg
    11461 gcctggttca aagcgtgaat ggcttcttca cgctcaaggc cgctgatttc gaacatgatc
    11521 ctgccaggct tcaccaccgc gacatagtat tccacgttgc ctttgccttt gcccatacgg
    11581 gtttcgggtg ggcggtgggt atagggttta tcagggaaga tccggatcca caccttacca
    11641 tggcgtttca ttgaatgcac gatggtgcgg cgggcggctt cgatctgccg ggcggtcacc
    11701 caaccaggtt ccaccgcctg gagtgcgtac tcacccacga tcatgtctgc gccgcgctgc
    11761 gccttgccgg tcatgcgacc gcgcatcact ttgcgccatt taacacgttt tggcatcaac
    11821 atagctgcta cctctttcac aatacccggg agttttccgg ggttagaatc tattcactca
    11881 cgtacacacc ctcggtgggt tcaaccttct cttcaacacc ggggatcact tcacctttat
    11941 aaacccaaac cttcacaccg attcttccgt acgtggttaa cgcttccgcg cgggcaaagt
    12001 cgatatccgc acgcagcgtc tgaagtggta cgcgcccctc acgcagccag acgatacgtg
    12061 ccatttcagc gccgctcagg cgaccagaga ccgagacctt aattccctcg gcgccctgcc
    12121 gcatggcttg ctgcagcgcg cgcttcatcg cgcgacgata gcttaccctg cgttcgatct
    12181 gaccggcgat gttcttagca acgagaaacg cgtcacaatc cggcgctttg atctccttga
    12241 tctcgaggtc aatcgccttg ccagtaagcg cttcaagatc agtgcggatc ttttttacag
    12301 aatctccctt gcgcccaata aggatgccgg gtttcgcggt gtgcaccact acctttacct
    12361 tgcccgggaa ccgctcgatc tcaaccaacg agattcccgc cttttctccc tggctctgaa
    12421 ccatcctgcg gatgaccaga tcctggtgaa gctgggtgcg gtaatcagaa ccctcggcgt
    12481 accaacgccc ttcccagggc tggttgattc caagacgaaa accaattgga tgtacttttc
    12541 gacccataat ttctcctggt gtcctttttc actcagacgg aggttttccc tgccgccctc
    12601 ctggtatcta ttccttctct ctcagcacaa cagtgatatg agaggagcgt ctcaacaacg
    12661 gcttgaagcg tccgcgggcg ccaaatcggc gccatttgcg aagaggggca tcatcagcgc
    12721 tgatcttgta aaccaccaga tcatcacggt tgacgccgaa gttctcttca gcgttcgcca
    12781 cggcggaagc cagcaatcta cccacatgcc ttgcagctgc cttattggta aatttgagag
    12841 tggtcagcgc ttcaactgcc ggtttgccgc ggatcagatc gataaccagg cgcgttttct
    12901 gagcagaaac accacaattc ttcaattctg cgcgaatatc tgttgccata attatctcgc
    12961 tccggtagat ttatcagaca tgtgtccccg gaaatgacgc gtgggggcaa actcacccaa
    13021 acggtgaccg accatattct ctgtgatgta aatgggaaca tgccggcgcc catcatgcac
    13081 agcgatggtg tggcccacca tttgcgggaa gatcgtgctc gccctgctcc aggtgcgaac
    13141 cactttcttc tcgctgcggg tgttcatggc ttcaattttt ttcaatagtt tcgggtcaac
    13201 gaacggccct ttttttagcg atctagacat attctcgtcc cttcttctcg gttaagcgct
    13261 gcggcttgtt ttaccgattg ccggcgttgc ggcggcgcac aatatactgg tcggtctttt
    13321 tgttacgccg ggtgcgatac ccgcgcgtgg gcttacccca cgggcttttc ggcccagaca
    13381 taccgaccgg ctggcgtcct tcaccaccgc catgaggatg gtcgcggggg ctcatagccg
    13441 tgccgcgcac ggtgggcttg atgcccatat ggcgtttcct acccgcctta ccgagcttga
    13501 tgttgctatg gtctgtgttt ccaacctgcc cgatagtggc gtaacatttt tggggaacca
    13561 ggcggacttc gcctgacggc atacggattt gagcgaaatc gccctctttt gccaataact
    13621 gcgcagacgc gcccgctgag cgcaccaact gcccgccttt cccttctttc agttcgatat
    13681 tatgaacaag ggtgccgacc ggaatattgg aaatgggcag gctgttgcca ggccttacct
    13741 cagccattgg accggctacc actgtatcgt ttactttcag accaaccggc gcgatgatat
    13801 aacgtttctc accatcaaca taagtcagca gagccagtct ggcagtacgg ttgggatcat
    13861 attcaatcgc ggccacgcgt gcgggaattt cccgcttatc acgtttaaaa tcgatgatgc
    13921 ggatatactg cttgctgccg ccgccctggt ggcgcacagt cacttttcca tgattgttgc
    13981 gcccagcccg ggctttgcgg atcacgatca aagcgcgctc aggcgtgtct ttggtgattt
    14041 cttcaaagct gtaaccggtc attgcgcgcc ggctgggtgt ggttggtttg tataccttaa
    14101 tagccattac tccacccctt caaagatggg aatacggtct tcagggctga gcgtgacgac
    14161 cgcttttttg tactcagcgt tgcggatcaa tagccttcgg ctgcgcgcgc ggcgggacct
    14221 tttcgccggc gcattgatga tattcacccg caaaacattc acgttgaaca atttttcaac
    14281 cgcgtctttc accattgtcc ggttggcatc atctgccacc tcgaagacgt actgatgaag
    14341 tttcgtgacc atgtaattgg ttttttcggt cacaatcggg cggcggagga catcgtaaat
    14401 agtgctcatc ttattctcct acccctccca ggttagccgt aattaactca agggcggcga
    14461 caggcatgat cagcttatca aagaccataa tgtcgcgcac attgatgtaa ttggcattca
    14521 ataccttggc gtcagggatg ttattggtgg agcgcagcac atcctcgtag gattctgctg
    14581 aaggaatcaa cacaagggct ttggcatcgc caaccagcgt attcaacgcg ctggccatta
    14641 aacgcgtctt gggttcctta agtttgaggg cgtccaccac cacaattgag gattcagccg
    14701 ctttcacaga gagagctgaa cgcagcgcgg cctggcgcat tttcttcggc atgttttgaa
    14761 cgtaagagcg catatgcggg gtatgaatct taccgccgcc tttccactga gccgatctag
    14821 tagaaccctg tctggcacgc ccggttccct tttgccgcca tggttttctg ccaccgcctg
    14881 aaacctcgct cttgaccttg gtctcgtgcg ttccaaggcg agcgttcgcc atttgcatca
    14941 cgtaagcctg atgcatcaga tcaatattga ttggggcttc aaagatcgcc ggtggcagtt
    15001 ccaccttact gacctttttc ccttccatgt ttaagacttc aacttccatt gtcatgagct
    15061 cccttagtcc aggtaaacgg ataaaaaacc tcggacctaa cctttccgcg attctttaat
    15121 gagaacgagg ccaccgcgtg ccccaggcac ggagccattg acaccgatga cattgcgttc
    15181 cgcgtccacc aggacgacct tcaaattctg cgcggttaca gcgtcattgc ccatatgacc
    15241 ggcaccgcga gcgcctttga atactctgcc aggtgtggtg gttgaagaac gcgaacccgg
    15301 cgcccgcata cggtctgact gaccatgcga ttttggacca ccgcggaaat gatagcgttt
    15361 tacaccgcct gcaaatccct tccccttgct gattcccgag acgtccacct tttcaccaac
    15421 cgcgaacagg ttcacatcca ctttgtcgcc ttcagcaacc tgaacctctt tggcccgaaa
    15481 ttcgcgcagg tattttaatg ggggaaggct attgcgtcgc aagtgaccta actcgccgct
    15541 ggccagacgc ctgggtttta cttcttcgta acccagctgc accgcggagt aaccttcctt
    15601 ctgcacggtt ctcacctggg taacgaagca tggcccagct tcgattagcg tgacagggtg
    15661 cgccgcgcca ttttcatcga aaatctgggt catgccgatt ttctttccga tcagcccttt
    15721 aaacatttca gttgttctcc ttcagtactt tagtagagac tgtcagcctg ggcttggctg
    15781 catcatccct gaacaattca gttagcagtt tgctgcgaca ctatcggttt ttgtctggtg
    15841 cgcccacaag aaccgctctt gaattgcctg cttgggctta cgcccaaaac cgatacatta
    15901 taccgcaagt tccggtattt gtaagggttt ttttaatttt taaggtgatc taccgcacaa
    15961 tgcaaagttc agatgcgcag taaggtctta gatcttgatc tcaatatcca caccagccgg
    16021 catgttcaaa cgcatcaaca tgtcaatcgt cttggcgtca ggatcgagca catcgatcag
    16081 gcggttgtgc gttctgatct caaaatgctc gtgagaatct ttatcgatga atgatgaacg
    16141 gcgaacagtg aaccgttcaa tgcgggtcgg cagtggaaca ggtcctacaa cctgcgcgcc
    16201 actccgctcc gcggtctcga caatcctttt ggcagattga tcgagcacac gatgatcgta
    16261 ggccttcaag cgtatccgaa ttttttgttt tgccatgtta ttaacctatg caatgatttt
    16321 tgtgatcctg ccggcgccaa ccgtcagtcc accttcacga atcgcga
//
LOCUS       DAOSPM010000042        15960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_3699_length_15960_cov_13.424018, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000042 DAOSPM010000000
VERSION     DAOSPM010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15960
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3699_length_15960_cov_13.424018"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLW25_05215"
     CDS             <1..825
                     /locus_tag="PLW25_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOS53924.1"
                     /translation="DVFYGIDNTYLSRALQEQIFVPYTPAALAQLPGEFKSGLSGMVT
                     PIDFGDVCINYDKAWFTERGLALPATLADLAKPAYKGEAGNGLLVVENPATSSPGLAF
                     LLVTIAEYGETGYLDYWRSLKQNGVVVVNDWETAYYTNFSASSGRGLQPMVVSYATSP
                     AAEVIYASEPLNEAPTASITTANTCFRQIEYAGILKGTGQLELAQRFIDFMLSPVFQE
                     DLPLQMFVFPVNPAATLPDDFLQYAAFAQEPARIDPVLISKNRDKWVTEWTRVMLP"
     gene            840..2516
                     /locus_tag="PLW25_05220"
     CDS             840..2516
                     /locus_tag="PLW25_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOS53925.1"
                     /translation="MTRSVKQSGVLLWGAPLALLAVFFFIPMAAILNVMFAQSGDIVF
                     SDSLRPLGFTVFQALLSTLLTLLLGLPAAYVFARFDFTGKRGLRMLTMLPFVLPTMVT
                     AAAFNALLGPRGWVNLLMMKLTGAQAPPINFLNTFTAILVAHVFYNIAIIIRVVGNAW
                     SQLDPRLENSARVLGASPWRAFIEVVYPLLRRPILSAALLVFLFDFTSFGVILMLGGA
                     GFSTLEVAIYTQALHNLNLPMAGLLSLIQLACTVIILLLYGWSSQGNDVPLFPRLSGE
                     GTRKPRSAVEKGTVALMVIILFTLQVLPLFALVSRSLVRLEGERGQRGKVQSGLTLDY
                     YHELFVNRQQSVFYVPPSRAVINSLSYAGITMLLSVGAGTLLAYAMVKRPATRRWLDP
                     LVMLPFGASSVTLGLGFLIAFSGSMLSRASFPWLVPLAHSLIAIPFVLRTVQPALSVM
                     PKSLCQSAAVLGASPARAWWEVEMRLLSRPLLVGGLFAFTISIGEFGATTFIARPDNP
                     TVPVAIFRYLAQPGGLNYGQAMAMATILIVICAVSIGLLESLSRAINGSE"
     gene            2527..>3507
                     /locus_tag="PLW25_05225"
     CDS             2527..>3507
                     /locus_tag="PLW25_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53926.1"
                     /translation="MDRLEIIDLHKMYEGAPLLDGVSFYVEAGETLCLLGSSGSGKST
                     ILRIIAGIETQDAGSILWRGKDVSAVPAHRRGFGLMFQDYALFPHLNVSENIAFGLRM
                     QHAAKDQLGARVFEALERVNMTAFASRRVTDLSGGEQQRVALARALAPRPQLLMLDEP
                     LAALDRSLRVELQEDLRELLHQTGIPAIYVTHDQEEAILLGDRLALLHNGRIVQSGLT
                     EEVYRQPANTWVARFLGMTNLLEGRVVSVNPLMVQTACGTLGPFVPGYPSLLSPGQVV
                     HLLVRLDGLFSGEHQPSAGSINGEVTECRFMGDHYRVRMKVGECASLECFT"
     assembly_gap    3508..3607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3777..3944)
                     /locus_tag="PLW25_05230"
     CDS             complement(3777..3944)
                     /locus_tag="PLW25_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53927.1"
                     /translation="MTQPEKELPDVKNQELLASRKRQVWQVVLVMTLSFCIGIVLFML
                     ILWWVFQKISP"
     gene            complement(3941..4516)
                     /locus_tag="PLW25_05235"
     CDS             complement(3941..4516)
                     /locus_tag="PLW25_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOS53928.1"
                     /translation="MDIFNIFTAFGLSASAGLNAYIPLLVVSLLAKFTNLIKLQEPWN
                     LMTSWWVIGVLIVLTIVEILADLFPGVNHVNDVIQSFIRPTAGAVVFAATSSQVGDVN
                     PILGLIAGLLVSGGVHAVKALAVRPAVNAATGGLGNVPASIGEQATSTVVSILSVIIP
                     VIVAITLTIVLIIIILWANNRKKKRETQPVE"
     gene            complement(4534..5328)
                     /locus_tag="PLW25_05240"
     CDS             complement(4534..5328)
                     /locus_tag="PLW25_05240"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOS53929.1"
                     /translation="MSEKKDDTPQKFRWLNPALVLLLVALFSAWLLLTPPGIEGKMNA
                     IGFSVCHQIDSHSFSVGGKVLPLCARCTGTFLGLFISMVYLYSKDRKSGFPSRQKITV
                     LLFFFAFFAFDGINASLPLVGLKTVYTPSNQLRLISGLLFGITLANVLLPLWHQTLWS
                     NPNPQPVWHSWQQLGWLFLFCALAGVMVVSDVPILFYPIAILSTLTIYLVLGMIYTLL
                     WCIILKKENSLHLFRDGFRILSAGLITAITQVGLMDLIRFSLSQTW"
     gene            5772..7001
                     /gene="pepT"
                     /locus_tag="PLW25_05245"
     CDS             5772..7001
                     /gene="pepT"
                     /locus_tag="PLW25_05245"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002448057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOS53930.1"
                     /translation="MPTVKERFIKYARINTESNRDSETCPSTQAQFDLARVLESELRE
                     MGLQEISLDENCYLMASLPANTEKPLPVIGFIAHLDTSPDMSGAQVNPQIVASYDGGD
                     IVLNHELGIQMKPMDFPELQKYIGQELITTDGTTLLGADDKAGVAAIMSAMEHLVAHP
                     EIKHGKIRVGFTPDEEIGRGADKFDVEKFGASYAYTVDGGEIGELEYETFNAAEVQVA
                     VSGRNVHPGVAKNKMVNSILLAMEFNAMLPVNERPEFTEGREGFIHLLGVSGSVENTR
                     MYYIIRDHDRMLLEKRKQYMIDAATLLNRRYGEGRIGLEINDQYYNMREKIEPVFEIV
                     ENARDAMLALGIEPKILPVRGGTDGSRLSFMGLPTPNIFTGGLFAHGKYEVLPTRSLE
                     RAVEVIIKIIEMAGKDK"
     gene            complement(7027..7896)
                     /locus_tag="PLW25_05250"
     CDS             complement(7027..7896)
                     /locus_tag="PLW25_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOS53931.1"
                     /translation="MFTLLVVSVIWAFSFGLIKGNLTGVDSNFVAFARLFIAALVFLP
                     FIKWKKIQTKTAIKLALTGLVQFGMMYIAYLAAFRTLKAYEVALFTIFTPIFVTLIND
                     AFMKHINLLHLAVALITVAGTAIIEWESIQTPAVWNGFLLVQLSNLCYAFGQIYYRRL
                     MAQHPELKDQDVFGYLYLGATVITAAATLFTTPFNTLELHSKQSLTLLYLGVVASGVA
                     FFLWNIGARRVNAGALAVFNDLKIPLAVAVSLLVFGESANLPRLLSGGGIVIGALVIN
                     EWLSKRNLVKITG"
     gene            complement(8137..8820)
                     /locus_tag="PLW25_05255"
     CDS             complement(8137..8820)
                     /locus_tag="PLW25_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53932.1"
                     /translation="MKPESGISKTLRFIGILFMALTAAVTVLAGVGTSCVAFLPNNWG
                     PSMAKIAPMQWLYILYVLLTIAIGVLGIRAVVLLAKGRQGAYKAALIALVAGLVVGVV
                     HMITSRMLRGSSMPVDGIVYITVLTLVIFLVFRIPAIWNGVDYSKAPRKEGKRSSGAA
                     AIVAGALCLTIQFFMAPTHTWGGVNYADAFNTSMTIVGSGLVLLGIGLILSSVLVLAP
                     MKRKVDTLR"
     gene            complement(8844..9695)
                     /gene="cyoE"
                     /locus_tag="PLW25_05260"
     CDS             complement(8844..9695)
                     /gene="cyoE"
                     /locus_tag="PLW25_05260"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOS53933.1"
                     /translation="MKLLHKFNHYWSLIKSLQTALLTVTGIAGYMSARCPFTHWTTMT
                     GLIASLLLSISGSTVLNMWYDRDIDSVMNRTHHRPLASGTISPSEALWLGLGLSAAGV
                     ILAAALSPLYGLLIFAGIFFDVIVYTILLKRRTCWSVVWGGIAGGMPILAGRALGMGQ
                     VDLIGILLALAILFWIPTHILTFSMRYQQDYDKARIPTFPSTYGEKVTRRIVALASLV
                     AAGLIGTAAVMVGVKQGALQVLGILSGGLLVMAVLMLFKPNSRLNFHLFKYASVYMLA
                     SMVLLAF"
     gene            complement(9702..10607)
                     /locus_tag="PLW25_05265"
     CDS             complement(9702..10607)
                     /locus_tag="PLW25_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOS53934.1"
                     /translation="MKKSIITMSLFLLFALTACQPVDFQAAPPVFDTGMDSEQWVEIP
                     AGEFLQGQFKKETTIDHAYEIMVTPVTNAQYAAYLNEALAADYIQVNGNQVEGYYPGD
                     AFAGGRHEEEIKAGSYIHIPLDDPFLRLSYDGNSFTVKPGWENHPMTMVSWFGAKSYC
                     EFNGWRLPTELEWEKAARGTDGRAYPWGEEIARNNANFYFSRDPFENMATFGSRTTPV
                     GFYNGKTYNHYTTLDSASPYGVYDMAGNVWQWTANVYDAQHYRYMRGGSKDMYENNLR
                     VWSMNNATPTFTSPGTGFRCVRDVE"
     gene            complement(10607..12088)
                     /locus_tag="PLW25_05270"
     CDS             complement(10607..12088)
                     /locus_tag="PLW25_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53935.1"
                     /translation="MQWLNKRYQFLFRSTKGLILVAIAFAALGSAFFGLLSGPMADYG
                     IKDVVVRALNINLEPAEREGRLVILYHSLAMIAIAVEVYMITDLLKMKDNHRLPINTT
                     VTVGYLMVMFGGLPFAYWGHNWVLHGIYIAGLSLIFFAGCLLLVALWPWRDEYRLKDK
                     DYAQTKKGYDMERIAFFMVSLTTLISAIFGAVPGSFYGNGFETFLAENVVRDPHKTAL
                     DLSIIGHLHIMLALIAIMCALIIGRWMDFKGMWQKLAMPLYIVGAFVLSLGTWLVVQF
                     EEIAHFIIYGGSTVAMLGALFLVIDSWKKIIRSGLDEKGIRKGNFFQGVAALLRDPVR
                     FGPTWQMVFMNFTVSGVGIFTAVKLDEIFRKWQFMDERSILTGHWHILATLTATILLL
                     YYADLAGLKGKARKWFGWAVIIFSDVAFAAVTVYEMKRLFITEGEQQPLVNRAVLAGD
                     IGVIVLLLALAALMVWRLTDLFKKKGRWQEETTHRLAKKEEVQ"
     gene            complement(12200..12886)
                     /locus_tag="PLW25_05275"
     CDS             complement(12200..12886)
                     /locus_tag="PLW25_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOS53936.1"
                     /translation="MSETRELAERLKKITHFSEIPLSGVEQIIASGEIRQYVKGAFVF
                     HEGDESAGMFVLLNGQVNLSKLGPEGKEGILNTLEPVIMFNEVSALDGGPNPVSAYAH
                     TDIRLWRIGYLPFQQLLRQYPQIGISLLGVLARRNRLLLTHYDDLSFRTVQARMAKHL
                     ISLSENGRRSINRMENPIHTIAARIITSPEAVSRTLKVFKSAGVLRANRTEIEILDPR
                     HLQELAKIDL"
     gene            12943..14343
                     /locus_tag="PLW25_05280"
     CDS             12943..14343
                     /locus_tag="PLW25_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOS53937.1"
                     /translation="MTSFTFEKFIASYPGYTDTSYLDRLRELDYKRLEREKQVYLDYT
                     GGGLYADSQVIKHQRMLLEGVYGNPHSSNPTSSTSTALVEDTRARVLRFFNASPEDYV
                     VIFTANASGALKLVGEAYPFGQDCCYLLTFDNHNSVNGIREYALAKGAHVRYLPVLPP
                     RLRAVEGKLDEYLANARGSCHKLFAFPAQSNFSSVQHPLEWIDKAHAAGWDVLLDAAA
                     FAPTNRLDLSRWQADFVPISFYKMFGYPTGVGALIARRAALKKLRRPWFAGGTITVAS
                     VQGEKHYLADAPAAFEDGTLDYLNIPAVKIGLEHLESIGMELIHERVKCLTGWLLTCM
                     KDLRHRNGKPLVKIYGPSDTKQRGGAVTVNFFNPEGMVIDHRWIEEKANARNISLRTG
                     CFCNPGAGELALHISSEELSSCFNRPDHEQRLTYDEFRLCLNTKASGAVRISVGLVSN
                     FADGQRLLDFLATLVE"
     gene            14340..14909
                     /locus_tag="PLW25_05285"
     CDS             14340..14909
                     /locus_tag="PLW25_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53938.1"
                     /translation="MTDRMAALSHFLYRFSRGWVVLLGFIVFIGFSTLTLPGQNAIVD
                     EYSQGSGSPDTSLFYNAKQLYHMAEVYGQEGRTAYLNARWTFDVAFPFIYTFFLVSAI
                     GWLLNQSLPVGSRWRLINLVPLAALGLDFLENTATSVVMARYPHHCSLAEWLAPVFTL
                     LKWLLVGGSLVVLLVAIFLVLLRRIKKNA"
     gene            complement(14966..15664)
                     /locus_tag="PLW25_05290"
     CDS             complement(14966..15664)
                     /locus_tag="PLW25_05290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOS53939.1"
                     /translation="MAHLLGTERERYVKKTFKSVARRYNMMNHLMTFGVDILLRKEVM
                     RIAKLRNGETLLDIGAGTGDLSREARHTCPGAGINAVDFSLEMMAAGKDWKNIHRCQA
                     DALALPFSHNTFDVVVSGYLVRNVVDLDAALAEQYRVLKSGGRLVVLDTTRPRWKPLN
                     PLIKFYFRYVIPNLGRLFTGNGEAYTYLTQSSENFISAEDLADSLRRCGFTGVNFRVR
                     MLGTMAIHSASKRP"
BASE COUNT         3721 a         4106 c         4257 g         3776 t
ORIGIN      
        1 gatgtgttct atggaattga caatacttac ctctcgcgcg ccctgcagga gcaaattttt
       61 gtgccataca caccagccgc gctcgcgcaa ctgccgggag aatttaaaag tgggctttct
      121 ggcatggtga caccgattga ctttggcgat gtatgtatca attacgataa agcctggttt
      181 acagagcgcg gattggcgct gccagcaacc ctggcagacc tggcaaaacc tgcgtataaa
      241 ggtgaagcag ggaacgggtt gctggttgtg gaaaaccccg ctacatcttc accggggctg
      301 gcctttctgc tggtgaccat cgcggagtat ggggaaactg gctacctgga ttactggcgt
      361 tcactgaaac agaatggcgt ggtagtggtg aatgactggg aaaccgctta ctataccaac
      421 ttctctgctt cttcagggcg cgggttgcaa cctatggtgg tgagttacgc caccagcccg
      481 gcggctgagg tcatttatgc ttctgagccc ttaaacgaag cacccactgc ctccatcacc
      541 actgcgaaca cctgttttcg ccagatcgag tacgctggga tcttgaaagg caccggtcaa
      601 cttgaactgg cgcaaaggtt tattgatttt atgctcagcc cggttttcca ggaagacctg
      661 ccgctgcaga tgtttgtttt cccagttaac ccagcagcca cgctgccaga tgattttttg
      721 caatacgcag cgttcgcgca ggagcctgcc aggattgacc ctgtgctgat ctcgaaaaac
      781 cgggacaagt gggttaccga gtggaccagg gtaatgctgc cttgaggttc tggcaggagt
      841 tgacgaggtc agtcaagcaa tctggcgtgc tgttatgggg agcgccgttg gcattgttag
      901 cggtcttctt ttttatcccc atggcggcca ttctgaacgt catgttcgcg caaagcgggg
      961 atattgtatt ctctgattcg ctgcgaccgc tgggttttac tgtttttcag gcgttgctct
     1021 ctacattgtt aacccttcta ttgggattgc ctgcggcgta tgtttttgct cgtttcgatt
     1081 ttacaggcaa gcgtggctta cgcatgctca ccatgctgcc gttcgtgttg cccaccatgg
     1141 tcaccgcggc ggcgtttaac gcgctgctcg gaccgcgtgg ctgggtcaac ttgcttatga
     1201 tgaagttgac cggcgctcaa gcgccaccga taaacttttt aaatactttt acggccattt
     1261 tggtggcgca tgttttttat aatattgcca ttatcatccg cgttgtcggt aacgcctggt
     1321 cacaacttga cccgcgtctt gaaaacagtg ccagggtact gggggcttcc ccgtggcgcg
     1381 cctttattga agtcgtttat ccattactgc gccgtcctat cctttctgcc gccctactgg
     1441 tattcctttt tgatttcacc agcttcggcg tcatcctcat gctgggaggg gctggttttt
     1501 ctacgcttga ggtggccatt tatactcagg ctttgcataa cctcaacctg ccgatggctg
     1561 gtctactttc actcatccaa cttgcctgca cagtcatcat cctgctcctt tatggctggt
     1621 ccagccaggg taacgatgtg cccctctttc cgcgcttaag cggagaaggt acgcgcaaac
     1681 cacgttcagc ggttgaaaaa gggaccgttg cgctgatggt aattatttta ttcaccctac
     1741 aggtgctccc tttatttgcg ctcgtcagcc gttcgctggt ccgccttgaa ggtgagcgcg
     1801 gtcagcgtgg aaaggtacaa tccgggttga ccctggatta ctaccacgaa cttttcgtca
     1861 accggcagca aagtgttttt tatgttccgc cttcgcgcgc ggtgattaat tcactgagtt
     1921 acgcgggtat aaccatgctt ctctctgtgg gtgctggcac cctgctggct tacgcgatgg
     1981 tgaaacgccc agccacaagg cgctggctgg atcccctggt aatgctccca tttggcgcgt
     2041 cctctgtaac gcttggctta gggttcctta tcgccttcag cggcagcatg ctgtcacgcg
     2101 catcttttcc ctggctggta ccgctggctc acagcctgat cgccatcccg ttcgtgttgc
     2161 gtaccgtaca gcctgctttg tctgtcatgc caaaatcgtt gtgccagtca gcagcggtac
     2221 tgggtgcatc cccggcgcgc gcctggtggg aggtggagat gaggttgctg agccgccctc
     2281 ttctggtagg cgggcttttt gcgttcacca tatcgattgg tgagttcggc gccacaacct
     2341 ttatcgcgcg gccagacaat cccacagtgc ctgtagccat ttttcgttac ctggctcagc
     2401 ctggcgggtt gaattacggg caggctatgg cgatggctac aattctgatt gtcatctgcg
     2461 cggtgagcat tggtttgctt gaaagcctca gccgcgcaat caacggcagt gaataacgga
     2521 gggcgcatgg accgtttgga aatcatcgat ctgcataaaa tgtatgaagg cgcccctctc
     2581 ctcgatggcg tgagttttta cgtggaggct ggcgaaaccc tgtgcctgct cggcagttct
     2641 ggcagcggca agagcaccat tttgcgcatt atcgcgggta tcgaaaccca ggacgctggc
     2701 agcatcctct ggcgcggcaa ggatgtcagc gctgtcccgg cgcaccggcg cgggtttggg
     2761 ttgatgtttc aggattatgc gcttttccca cacctcaacg taagcgagaa tatcgctttt
     2821 ggtttgcgca tgcagcacgc agccaaagat caacttggcg cccgtgtttt tgaagcgctg
     2881 gagcgtgtca atatgactgc ttttgccagt cgtcgggtca ctgacctctc tggcggcgaa
     2941 cagcagcgtg tggcgcttgc ccgcgcgctg gcgccgcgac cacagctctt aatgctggat
     3001 gaaccgctgg cagcgctcga tcgaagcctg cgagttgagt tacaggaaga tttacgcgag
     3061 ttactccatc agacaggcat tcctgctatc tacgtgacac acgaccagga agaagccatc
     3121 ttgctgggtg accggctcgc cttattgcac aacggacgaa ttgtgcaaag tggtctcaca
     3181 gaagaagtgt accgtcagcc ggcgaacacc tgggtagcgc gctttttagg tatgaccaac
     3241 ttgctcgaag gcagggtggt cagcgtaaac ccgctgatgg tacaaaccgc ctgtgggacg
     3301 cttggcccgt tcgttcctgg atatccctcc cttttaagtc cgggtcaggt ggtgcacctt
     3361 ctggtcagac tggatgggct tttttcaggc gaacatcaac cttcagccgg cagcattaac
     3421 ggcgaggtta cggagtgccg cttcatggga gaccattacc gggtgcgtat gaaggtcggt
     3481 gagtgcgcca gtttggaatg ctttactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnncca ttaccgggtg cgtatgaagg tcggtgagtg cgccagtttg gaatgcttta
     3661 ctgcctcccc gcttgtactg gggaaggcgc ttgaattacg cctgaatgcg gaagatgttg
     3721 ttgtgctgcc cgaataaaaa cgcttccact ggcagtaaaa cctacggcgc tggcattcaa
     3781 ggggaaattt tctggaaaac ccaccacaag atgagcatga acaaaacaat gccaatacag
     3841 aaggagaggg tcatgaccag cacaacctgc catacctgcc ttttccggct ggcaagcagt
     3901 tcctgatttt tcacatctgg cagttctttt tctggctggg tcactctacg ggttgggttt
     3961 ctcgtttttt cttacggtta tttgcccaaa gaataataat aatgagaacg attgtcagag
     4021 tgatggccac gatcaccggt atgatcacgg agagaatcga aaccactgtc gaggtggctt
     4081 gttcaccaat cgaagcgggt acgttaccca aaccaccggt ggcagcgttt acggctggtc
     4141 gcaccgccag agcttttacg gcgtgtaccc cgcctgaaac caacaggccc gcgatcaagc
     4201 ccagtatagg gttcacatca cccacctgac tggaggtggc tgcaaataca accgccccag
     4261 cggtaggtcg aatgaacgac tggatgacat catttacatg gttaacgcct gggaatagat
     4321 ctgccagtat ctccacgatg gtcagcacga taagcacgcc gatcacccac caggaagtca
     4381 tcaggttcca gggttcttgc agtttgatca gattggtgaa tttggcgagc agcgagacga
     4441 ccagcagagg aatataggcg ttcaaaccgg cgctggcaga gaggccaaac gctgtaaaaa
     4501 tattgaaaat atccattaat tcatttcctt ttcctaccag gtttggctga gagagaagcg
     4561 gatgagatcc ataagcccaa cctgggtgat agccgtgatt aaaccggcac tcaatatacg
     4621 aaaaccatcg cgaaaaagat gcagcgagtt ttcctttttt aagatgatac accaaagcaa
     4681 tgtgtagatc atgcccagca ccaggtagat ggtcagggtg gagaggatgg cgatagggta
     4741 aaacaggatg ggcacatcgg ataccaccat caccccagcg agggcgcaaa ataggaataa
     4801 ccaaccaagc tgctgccagc tatgccagac tggctgcggg ttcgggtttg accatagtgt
     4861 ctggtgccat aacggcagca acacatttgc cagcgtgata ccgaagagca acccgctgat
     4921 caacctgagc tggttggagg gtgtatagac ggttttaaga cccaccagcg gaaggctggc
     4981 gttgatgccg tcaaaggcga aaaaggcgaa aaagaacaac agcaccgtta ttttctgcct
     5041 ggatgggaaa ccgcttttac ggtcttttga gtacaggtag accatcgaga tgaataaacc
     5101 caggaaagtg ccggtgcagc gcgcgcataa cggcagcact ttccctccga cactaaagga
     5161 atggctgtcg atttggtggc agaccgaaaa ccctatcgcg ttcatcttgc cttcgatacc
     5221 cgggggcgtg agcagcagcc acgcagaaaa aagcgctacc agcagcagca ccaacgcagg
     5281 gtttagccag cgaaattttt gaggggtatc gtcttttttc tcagacatag ggcaaattat
     5341 aaaggggata ggaggttgga gcaagccata gtcggagggc agtgggtgac cgcgcccgct
     5401 tgacgcgaat gcggaattat gattaactta accagaagaa cttgccatcc taacgatgag
     5461 gaggtgatgt ctacgatgtg taccagtttt gaacacagcg ctgtggcatc cctcctcgcg
     5521 atagagtagg gaagccgcag cgccaaaccc ccggagccgt cagcgtgacg gctctgtggt
     5581 tttcctggat taattcactc tttaaccgct gcacccttaa aagaaaagtg gaaagcgcca
     5641 ccagcacaag ttggcaatat cacctcaagc gtttacggca agggcgctta accgtacgcg
     5701 gcaagagttg cgtataccag tcaacccacg cttcggtata taatcagttc atcaagctac
     5761 caggagggcg catgccgaca gtaaaagaaa gatttatcaa gtacgccagg atcaacacgg
     5821 aatcgaatcg tgacagcgag acctgcccca gcactcaggc gcagtttgac ttggcgcggg
     5881 tgctcgaatc agaattgcgt gaaatgggtt tgcaggaaat aagcctggat gagaactgct
     5941 atctgatggc ttcactaccg gcgaacactg aaaagcctct ccctgttatc ggcttcatcg
     6001 cgcatctcga caccagtccg gatatgagcg gcgcgcaggt taacccccag attgtcgcca
     6061 gctatgacgg gggtgatatt gtgctcaacc atgaactcgg tatacaaatg aagccgatgg
     6121 acttcccgga actgcaaaag tatattgggc aagagttgat caccacagat ggcaccacct
     6181 tgctcggggc ggacgataag gctggtgtcg cggcgatcat gtctgcaatg gagcacctgg
     6241 tcgcgcaccc ggagatcaag cacgggaaaa tccgtgttgg cttcaccccg gatgaagaaa
     6301 ttgggcgcgg cgcagataaa tttgatgtgg agaaattcgg tgccagttac gcatacacgg
     6361 tagatggcgg agaaattggc gaactggaat acgaaacctt caacgctgcc gaggtgcagg
     6421 ttgccgtaag cggcaggaat gttcaccctg gggtggcgaa aaacaagatg gtcaattcga
     6481 ttctactggc tatggaattc aatgccatgc tgccggtcaa tgagcgcccc gaattcaccg
     6541 aagggcgtga agggtttatt catttattgg gtgtatccgg ctcggtagaa aacacccgca
     6601 tgtattatat tatccgtgac catgaccgga tgctgcttga aaaacgcaag caatacatga
     6661 tcgacgcagc caccctgctc aaccggcgtt acggcgaggg acggattgga ctggagatta
     6721 acgaccaata ctataacatg cgcgagaaga tcgaaccggt ttttgagatt gttgaaaacg
     6781 cgcgtgatgc catgctcgcg cttggcatag agccgaaaat attgcccgtc cgcggcggca
     6841 cggatggttc gcgcttgtct tttatgggat taccaacacc caacattttt accggagggc
     6901 ttttcgctca tggcaagtat gaagttctgc ccactcgttc actagagcgc gcggttgaag
     6961 tgatcatcaa gatcatcgaa atggccggta aagataaata acccgctcgc gagagcggtt
     7021 tatttatcaa cccgttattt tgacgaggtt ccgtttactg agccattcgt ttattacgag
     7081 cgcgccaata acgatgccgc cgccactgag cagccgaggg aggttggcgc tttcaccaaa
     7141 gacgagcagc gaaaccgcca cggcgagggg aattttaaga tcgttgaaga cagccagcgc
     7201 gcccgcgttt acccggcgcg cacctatgtt ccagaggaag aaagccacac cggaggcgac
     7261 gacccccagg taaagcaggg tgagactctg tttcgaatgg agttcgaggg tattgaatgg
     7321 cgtggtgaac agcgtcgccg ccgcagtgat gaccgttgcg cccaggtaaa gatagccaaa
     7381 tacatcctga tcttttaact ctggatgctg cgccatcaac ctgcggtaat aaatttgccc
     7441 aaaagcgtaa cacaggttgg aaagttggac cagcagaaac ccattccaca ctgcgggcgt
     7501 ttgaatggat tcccattcaa taatggcggt gcctgctacg gtgatcaatg ccacagccaa
     7561 atgcagcagg ttaatatgtt tcataaaggc gtcattgatc agcgtgacga agataggggt
     7621 gaatatggtg aacagcgcga cttcgtaagc cttgagtgtt ctgaaggcgg ccaggtaggc
     7681 gatgtacatc atcccaaatt gcaccaatcc ggtgagcgcc agtttgatag cggtttttgt
     7741 ttgaattttc ttccacttga tgaatggaag aaaaaccagc gcggcaatga acaggcgcgc
     7801 gaaagccacg aagttagagt caacccctgt caggttgcct ttaattaatc caaatgaaaa
     7861 agcccaaatg actgaaacaa ccagtaaggt aaacatcaga ttattccaga ttctcctttt
     7921 ttatcgcggc aatcataaca cattcatgaa ccagcctata ccgagttttt tacttcggaa
     7981 ctgtcagcag aagaaagcgt aacttccaat atctgccctc tagtttcctt taagggatgg
     8041 gaagaataca aaattccctg ctgagaagca tcaccgggga tgaaaaagaa ctggcatcag
     8101 ccagttcttt tgttctgaaa aagggagagg atgaggttac ctgagcgtgt ctactttgcg
     8161 cttcatggga gcaagcacca ggacgctgct gagaatgagc ccgataccca gcaacaccag
     8221 cccgctgccg acaatcgtca tgctggtgtt aaacgcgtcg gcgtagttta ctccgcccca
     8281 tgtgtgggtt ggtgccatga agaactggat ggtcaggcac agcgctccgg cgacgatggc
     8341 cgcggctccg ctggatctct ttccttcttt acgcggcgct ttgctgtaat ctaccccgtt
     8401 ccagatggct ggaatacgga agacgaggaa aatgaccagg gtcaacacag tgatgtacac
     8461 gatgccatcc actggcattg atgaacccct cagcatgcgc gaggtgatca tatgaaccac
     8521 ccccacaacc aggcctgcga ccagcgcgat cagcgccgct ttgtaagcgc cctgtcgtcc
     8581 ttttgccagc aataccactg ccctgatgcc caacaccccg atggcgatgg tcaacaatac
     8641 atagaggatg tagagccatt gcattggcgc gatctttgcc atgctcggtc cccagttatt
     8701 gggcagaaac gccacgcagg aggtgcccac gccagccaat acggtgaccg cagccgtgag
     8761 cgccataaac aaaatgccga tgaatcttaa tgtttttgaa atgccacttt caggtttcat
     8821 aggaattcct tttcaaaaaa agattagaat gccagcaaca ccatggatgc cagcatatat
     8881 accgaagcgt atttgaacaa atgaaaattg agcctggagt ttggtttaaa gagcattaaa
     8941 acggccatca ccagtagccc tccagaaagg atgcccagca cctgcagcgc gccctgcttc
     9001 actccaacca ttactgccgc ggtgccgatt aacccagcgg cgaccaggct ggcaagggcg
     9061 acgatgcggc gggttacttt ttctccgtag gtggaaggga aagttggaat gcgcgctttg
     9121 tcgtaatcct gctggtagcg catactgaag gtgaggatgt gagttggaat ccagaacagt
     9181 atggccagcg ccaacaggat gccaatcaaa tctacttgac ccatgcccaa cgcccgtcca
     9241 gccaggatgg gcatgccccc ggcgatacca ccccaaacga ccgaccaaca ggtgcggcgt
     9301 tttaacagga tggtgtaaac gataacgtca aaaaagatgc cggcgaatat taacagccca
     9361 tacaaaggcg aaagcgctgc agccagtatg actcccgcgg cagaaagacc cagcccaagc
     9421 cacagggctt ctgacggaga gatcgtccca ctggccaatg ggcggtgatg ggtgcggttc
     9481 atcacgctgt caatgtcgcg gtcataccac atgttaagta cggtgctgcc gctgatagag
     9541 agcagcaggc tggcaatcaa ccctgtcatc gttgtccaat gggtaaatgg acaacgggcg
     9601 ctcatatagc ctgcgatgcc ggtcactgta agcagcgcgg tttgcaggct tttaatgagc
     9661 gaccagtaat ggttgaattt atgcagcaat ttcatcaatc tttactccac atcgcgcaca
     9721 caacgaaaac cggttcccgg gctggtgaat gttggcgtgg cattgttcat tgaccagacg
     9781 cgcaggttgt tttcgtacat atccttactg ccgccgcgca tgtagcggta atgctgagcg
     9841 tcatacacat tggctgtcca ctgccagacg ttgccggcca tatcgtaaac gccatagggg
     9901 ctggcggaat ccagcgtggt gtagtgatta tacgtcttcc cgttgtaaaa accaacagga
     9961 gttgtgcggg aaccgaaggt agccatgttt tcaaaaggat cgcggctgaa gtagaagttc
    10021 gcgttgttgc gcgcaatttc ttcgccccag ggataagccc ggccatctgt gccgcgcgcc
    10081 gccttttccc attccagctc ggtgggcaag cgccagccat tgaactcaca ataagatttg
    10141 gcgccaaacc acgaaaccat agtcatcggg tggttttccc atcctggttt cacagtgaaa
    10201 ctgtttccgt cataagagag gcgcaggaaa gggtcatcca gggggatgtg aatgtaactg
    10261 cctgccttga tttcttcctc atgtcgcccg ccagcgaaag catctccagg atagtaacct
    10321 tccacctggt tgccattgac ctgaatatag tcagcagcga gtgcttcgtt taagtaagca
    10381 gcatattgag cgttggtcac cggggtaacc ataatctcgt acgcgtgatc gatcgtggtt
    10441 tcctttttga attgtccttg caaaaactca cctgctggta tctccaccca ttgctcgctg
    10501 tccattccag tatcgaacac cggtggcgca gcctgaaaat ctacaggctg gcaggcggtg
    10561 agggcgaaaa gcaggaaaag ggacatggtg atgatcgatt ttttcattat tgcacctcct
    10621 cctttttggc caatcggtgg gtcgtttctt cttgccacct gcctttcttt ttaaacaggt
    10681 cagttagccg ccacaccatc aaggcggcca gcgccagcaa taacacgatt acgccaatat
    10741 ccccggctaa cacagcgcgg ttgaccagcg gctgttgctc accttcggtg atgaaaaggc
    10801 gcttcatctc gtaaacggtg acggcggcaa aggccacgtc tgaaaagata atgaccgccc
    10861 aaccgaacca ctttcgcgct ttccccttca accccgccag gtctgcgtaa tagagaagca
    10921 ggatggtggc ggtaagggtg gcgaggatat gccagtgacc ggtcaggatc gagcgctcgt
    10981 ccataaattg ccatttgcgg aaaatttcat ccagcttcac cgcggtgaaa atacccacgc
    11041 cgctgacggt gaagttcatg aacaccattt gccaggtagg accaaaacgc accgggtctc
    11101 tcagcagcgc agcgacgccc tggaagaagt tgcctttgcg gatgcctttt tcatccagcc
    11161 cgctgcggat gatctttttc cacgaatcga tcaccaggaa gagcgccccg agcatggcca
    11221 cggttgaacc gccatagatg atgaaatgtg cgatttcttc gaactgaacc accagccagg
    11281 tgccaagtga gagcacgaaa gcgcccacga tataaagcgg catggccagt ttctgccaca
    11341 ttcctttgaa atccatccac ctgccgatga tgagcgcgca catgatggcg atgagcgcca
    11401 gcatgatatg caggtgaccg atgatggaaa ggtccagcgc ggttttatga ggatcgcgca
    11461 ccacattttc ggccaggaag gtttcaaaac cgtttccata gaaagaccca ggcacagcgc
    11521 caaaaatcgc cgaaatcaag gtagtgagcg agaccatgaa gaaggcaata cgctccatat
    11581 cgtaaccttt ttttgtttga gcgtagtctt tatccttcag gcggtattcg tctctccagg
    11641 gccacaacgc caccagcagc aagcatcccg caaagaaaat aagcgacaga ccggcgatgt
    11701 agatgccgtg cagcacccag ttatgccccc aataagcaaa gggcagcccg ccaaacatca
    11761 ccatcaggta gccgacggta accgtggtgt tgattggcag acggtggttg tctttcattt
    11821 tcaataaatc agtgatcatg taaacttcca cggcgatggc gatcattgcc agcgagtggt
    11881 agaggatcac caatctgcct tcgcgctcgg caggttccag gttgatgttc aacgcgcgca
    11941 ccacaacatc cttgatgccg taatcagcca tcgggcctga gagcaagcca aagaatgcgg
    12001 agccgagagc ggcgaacgcg attgccacta agatcaaccc tttggtggag cggaagagga
    12061 actgatatcg tttattgagc cattgcattt tacttctccc gtgtttgtga ttaaccacta
    12121 cacaatataa catacccggc ggggataagt cattgacatt aatcaaaaag acttatgaca
    12181 aacagcggcg ccgcgatgct tataaatcga tttttgccag ttcctgcagg tgcctggggt
    12241 ccagaatttc aatttccgtt cggtttgctc gcagcacccc ggcggatttg aaaactttca
    12301 acgtgcggct tactgcttca ggagaagtga taatacgggc ggcgattgtg tgaatggggt
    12361 tttccatgcg gttgatggat cgtcgaccgt tctcgctgag agaaatcaag tgcttggcca
    12421 tgcgcgcctg aactgtacgg aacgaaaggt cgtcataatg tgtgagcaac aggcggttgc
    12481 gccgtgccaa cacgccaagc aaactgatgc caatttgcgg gtattgcctc agcaattgtt
    12541 gaaaagggag ataaccgata cgccataagc ggatatcggt gtgcgcgtat gcgcttaccg
    12601 ggttcgggcc tccgtcaaga gcggatactt cattgaacat gatgacgggc tcgagcgtgt
    12661 tgagaatgcc ttcttttccc tctgggccaa gcttgctcaa attgacctgt ccattgagaa
    12721 gaacgaacat tcccgcggac tcatcgcctt cgtggaaaac gaatgcgcct ttcacatact
    12781 gccgtatttc tccgctggcg atgatctgtt ctacaccgct caacggtatt tcgctgaaat
    12841 gggtaatttt ctttaaccgc tctgccagct cgcgtgtttc actcataggt caattatata
    12901 cattgtgcgg ataaaaattg gtttttatgg tttaatgcag tgatgacatc tttcacattt
    12961 gaaaaattca ttgccagcta ccccgggtac accgatactt catacctcga ccggctgcgt
    13021 gaactcgatt ataaacgcct ggaacgcgaa aaacaagttt acctggatta caccggtggc
    13081 gggttatacg cggattcaca ggtgataaag caccaacgca tgctcctgga aggcgtttat
    13141 ggcaacccgc attcttcaaa ccccacatct agcacctcaa cagccctggt ggaggatact
    13201 cgcgcgaggg tactgcgttt tttcaacgcc tcgcctgaag attacgtggt gatttttacg
    13261 gcgaacgcca gcggggcttt aaaactggta ggagaagcct atcccttcgg gcaggattgt
    13321 tgttacctgc tgactttcga taaccacaat tcagtgaacg gtatccgaga atacgcgctc
    13381 gctaaaggag cgcacgtgcg ctatctcccg gtgctgccgc cgcgtttgcg cgcggttgag
    13441 ggcaagctgg atgaatacct ggcgaacgcg cgcggctcat gccacaaact cttcgctttt
    13501 cccgcccaat ccaatttttc ttccgttcaa cacccgttgg agtggattga caaagcgcat
    13561 gccgcaggct gggatgtgct gctggacgcg gcggcctttg ctccgaccaa caggcttgat
    13621 ctctccaggt ggcaggctga ttttgttcct atttcctttt ataaaatgtt tggttatcca
    13681 actggtgtgg gcgcgctcat cgcgcgccgc gcagcgctga agaaacttcg gcgcccatgg
    13741 ttcgccgggg gtaccatcac tgtggcgtcg gtgcagggag aaaagcatta cctggcagac
    13801 gcccctgcgg cgtttgaaga tgggacgctg gattatctga acatcccggc tgtaaaaatt
    13861 gggctggagc accttgaatc cattggcatg gaattgatcc atgaacgggt gaaatgcctg
    13921 acaggttggt tgctgacctg catgaaagac ctgcgccatc gtaatggaaa accgctggta
    13981 aaaatctacg gcccttcgga taccaagcag cgcggtggcg cggtgacggt caacttcttc
    14041 aacccggagg ggatggtcat tgaccaccgc tggatcgagg agaaagccaa cgcccgcaat
    14101 atctccctgc gcaccggctg tttttgcaac cctggtgccg gcgagctcgc tctgcatatc
    14161 tcctctgaag aattatcttc ctgctttaat cgtcctgatc atgaacaacg cctgacttac
    14221 gatgaatttc ggctttgcct caacaccaag gcttcaggag cggtgcgcat ttctgtgggg
    14281 ttggtaagca atttcgcgga tgggcagcgg ctgcttgatt ttctcgccac gctggttgaa
    14341 tgacggatcg tatggccgcc ctttctcatt ttctttaccg tttttcccgg ggatgggtgg
    14401 tgttgctcgg gtttattgtg ttcatcgggt ttagcacgct caccctgcca gggcaaaatg
    14461 ccattgtaga tgaatactca cagggcagcg gctcgcctga cacctcgctt ttctacaacg
    14521 cgaaacaact ctatcacatg gctgaagtgt atgggcagga gggaagaaca gcgtatctga
    14581 atgcccgctg gacttttgat gtggctttcc cgttcatcta caccttcttc ctggtctccg
    14641 ctatcggctg gttgctcaat caatcgctgc cagtgggttc acgttggcga ttaatcaacc
    14701 tcgttccatt ggcagccctg gggctggatt ttctggagaa tacagccacc agtgtggtca
    14761 tggcgcgtta tccacatcac tgctcattgg ctgagtggct ggcgccagtt ttcacgttgc
    14821 ttaaatggct gcttgtcggt ggaagcctgg ttgtcttgct tgttgccatc tttttggtcc
    14881 ttctcaggcg aataaaaaag aacgcatgac cagccgctgg ctggatttat aaaaatgagg
    14941 tttttttcct gtgtaaatag ccgggctagg ggcgtttgct cgcggaatgg atggccattg
    15001 ttcccagcat gcgcacccgg aagttgacgc ccgtgaaccc gcagcgccgc agactatccg
    15061 ccaggtcttc ggcgctgatg aaattttcgc tggattgcgt caggtaggta taagcttcgc
    15121 cattgccggt gaacagcctg ccaaggttgg gaataacata gcgaaaatag aacttgatca
    15181 gcgggtttaa cggcttccag cgcggacggg tggtatccag caccacaagc ctgccaccgc
    15241 ttttgagcac ccggtattgt tctgccaggg cagcatccag atctaccacg ttgcgcacca
    15301 ggtaaccact caccacaaca tcaaacgtgt tgtgagagaa tggcagcgcc agggcgtctg
    15361 cctggcaacg gtggatattt ttccagtctt ttccggcagc catcatctcc aggctgaaat
    15421 caacggcgtt gatacctgcg cccgggcagg tgtggcgcgc ttcgcgtgac aggtctccgg
    15481 tccctgcgcc gatatccaac aacgtttcgc cgtttctcag tttggcgatg cgcatcactt
    15541 ccttgcgcag aaggatatcc acgccgaagg tcatcaagtg gttcatcata ttgtagcggc
    15601 gtgcaacgga cttaaaagtc ttttttacat accgttctcg ttcagttcca agcaggtgcg
    15661 ccattcgcct attataaatg gaattgtttt cccactttaa tcctgccagt ggaagctggt
    15721 gtgtgccttt cctgttgttc gagacgaggt aatgccatgt gtcccggttt tccgggagaa
    15781 tcacttggtt ttacttccct ttttgtagca ccctgttagg atagggggat tcgaatacag
    15841 cttggctggg ttgaatactc gaggattttg atgcccgaga aagggagagg agcggaaaag
    15901 aatcagtgcc cccaagggaa ttcgaatccc tgttttggcc ttgagagggc cacgtcctgg
//
LOCUS       DAOSPM010000043        15832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_3728_length_15832_cov_12.972111, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000043 DAOSPM010000000
VERSION     DAOSPM010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15832
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3728_length_15832_cov_12.972111"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..355
                     /locus_tag="PLW25_05295"
     CDS             53..355
                     /locus_tag="PLW25_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HOS53940.1"
                     /translation="MAESSLSNIDPVFPCRECQAGHMHRDYIAYFTWLGDELISVPDF
                     PAWVCDICGRCEYDENALTRLTLLLSPNAGKTIAPARLPRTKTRVKPKVQSPTQPD"
     gene            complement(383..2503)
                     /locus_tag="PLW25_05300"
     CDS             complement(383..2503)
                     /locus_tag="PLW25_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOS53941.1"
                     /translation="MTDIDPGFILKSRYKVLDLLGKGGMGEVYLAEDQSLANQVAVKV
                     NHNLSPNASAQFIREARLLASLKHPNLPRVIDYFTENDSQYLVMDFIPGENLRTIVEQ
                     KTPLSTPLILQWAEQLGNALSYLHSQNPPIYHRDIKPANIKITPKGEVILVDFGIAKT
                     GEASQETLSGAWGYTPGYAPPEQVSGLRTGPYSDQFSLAATLYYLFAGKPPADSAKRM
                     IGEEELNPLTQLNPTIPAHTASAIEKALSIKPEARFSTVQDFIASLCNTSPIPDPSSS
                     QNTLVGQRTVAAVGRGAPLVPPPSVAPPAQVAPALPAKKKSPLGWIIGALAVLGLGIA
                     AVLVLRNLSLRSTPSNEPPPTLTPMVIVITAQSPETPAPATEEPTAAQNIEPTSTAEP
                     LPAETEAPIYTSIGKGGKVAFVSNRQADGFDQIWLMDVMQNESGQLVGVNPVQLTFSE
                     GDKANPAWSPDGSKLLYSGFSKGFTANGNPLAMDIWLLDLSVPDAQAVDISLRQRDDK
                     YPSWSPVGDMIVYTSYYRDDGIPQLFMTDPQGEKQTRLSLPFAESYATWTPNAEFLLF
                     VQSTGNLNVVYMRDKYSQYTNGEHKFDRMSDEGRLGSVSQPNLSLDGALLAYTQTSGS
                     KTNIYVAPFADRGKTITALTSSGKDSWPCWSPDGQWLLFSSTRDGNAEIYIMDKNGEQ
                     VTNLTNLASQDKEPAWQPVPFPLP"
     gene            complement(2743..2952)
                     /locus_tag="PLW25_05305"
     CDS             complement(2743..2952)
                     /locus_tag="PLW25_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53942.1"
                     /translation="MFRKTSGLIGMIAGLVIVAVSLLADTLGIGTYPGLNNVQLAGIL
                     VGVAFFVIGYLLRRKKSKEKKPAAK"
     gene            3360..4355
                     /locus_tag="PLW25_05310"
     CDS             3360..4355
                     /locus_tag="PLW25_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOS53943.1"
                     /translation="MGLKYNDFAIRGIDVSEFNGAINWSKVQGHFAAIRVGHGRVIDK
                     RFTANWAGAKGKVDRLAYWYMDYYSHKDKSTSAYGISDKDWGRVQAEKCWSALKNDPE
                     GMVFLDIEKSSYGPALSTVQPRVLAIAQSFFDHYDKFSGKLNGIYCSLSMCTYFNEAL
                     RKRPLWVAWYNEAQNIDSVKKAVAARGWLGKCLIWQYASDGDVNDDGKADGTSLGMAY
                     GFLDLNGWLGTNAEYQQLFGKKAEVELYKVKILIYNLLVRNGPGLTYNRLRRADYPGE
                     YSIYEERDGYGRISQTLSEWISLDPAYVKKLGAAAVELTDAEKLARLWAAHPELH"
     gene            complement(4434..4505)
                     /locus_tag="PLW25_05315"
     tRNA            complement(4434..4505)
                     /locus_tag="PLW25_05315"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4471..4473),aa:Gly,seq:tcc)
     gene            complement(4585..6393)
                     /locus_tag="PLW25_05320"
     CDS             complement(4585..6393)
                     /locus_tag="PLW25_05320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOS53944.1"
                     /translation="MTAQTAPRWNLTNVYPSLSSKEYNADCEKVKSFISDMESLMEKA
                     AAFSGQEDASTLGALAQEMIACYNAILLVGGTIRAYIESFVSTDSYNQEALRKESEFD
                     LVAVKINQLETQLLGWIGKVKERLPEMIQSVPVLEEHRYFLEESARQSRYLMSEAEEM
                     LAAELSLSGSNAWTKLQGMLTSQASVEIEVDGELKRLPSPALINLRSHPDGETRKRGY
                     EAEDKLWTSLKVPLSAALNSIKGEVNVLNRRRGREDAVHSAIDAAHIDRQSLEAMLGA
                     MRDSFPMFRRYFKAKAKYLGKEKLPWWDLFAPVGEVKSDYSFIEAREVILRNFGRFAP
                     QLEAFARNAFEHNWIDAEQRDGKRGGAFCMEVPGVGESRILSNFDGSFDQVSTLAHEL
                     GHGFHNDCAVKAGKTEIQKFTPMTLAETASIMCETIVTSALLEEVTNPQQELAILESQ
                     LNGDSQVIVDIYSRYLFEKEVFERREKTELNADEICEIMENAQKATYGDGLDERYLQK
                     WMWTWKPHYYSGGLSFYNFPYAFGHLFATGLYAIYQQRGAEFVPNYIHLLASTGEAPA
                     ADLAARFGIDIRTRKFWEDSLRVSEKRVERYCELVS"
     gene            6537..7202
                     /locus_tag="PLW25_05325"
     CDS             6537..7202
                     /locus_tag="PLW25_05325"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOS53945.1"
                     /translation="MSEQFFNQERREVSGFSALVFRGMGRIDLLQSDHEELVIETQPE
                     IRSRIKTEVREGTLYIDYDENWKDWTGLRSLSGDKIVFKLMMREVTAISISGVGSLDT
                     PRIESANLSLAITGPGLLTVGTLKVPTLTVNLSGVGAIDVAGQTDELSVTLSGAGSLK
                     ASRLEAGKASVRLSGVGTATVWAKNSLETSISGAGVIEYYGSPQITQNNTGLGVLKFM
                     GNR"
     gene            7241..7834
                     /locus_tag="PLW25_05330"
     CDS             7241..7834
                     /locus_tag="PLW25_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOS53946.1"
                     /translation="MAQDRTRKIVVTAVLGAITILLGLTHWGFIPWFGGISLTIMHVP
                     VIIGAILEGPLVGAGIGLIFGLFSMLQAAIAPTGPLDPLFTNPLLAVLPRLFIGPVAW
                     LVWTALKKWPVVGLIASGIAGSLTNTVLVLSVLGFFFGRAPVMTQIFGEELWKPLSGI
                     AVVSGLPEAGVSALITLLVVAAYRQFSVGKRKGANLE"
     gene            complement(7864..8685)
                     /locus_tag="PLW25_05335"
     CDS             complement(7864..8685)
                     /locus_tag="PLW25_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOS53947.1"
                     /translation="MSEFDFLSRIPLGQYIPTNSIIHRLDPRAKIIMFGVLLIAITFT
                     PSQLGLSIGIAALLLALLLSKVPIGFALKGLLVPLPFLLIIAIIQVFFYSSALDPNIL
                     IKLGKLNITSGGLLAGVMLLIRFAGLILCLSLVSFVTSTSELLHGLTKILSPLNRVGI
                     QTMDFVMIVQVTLRFLPFLAQSAERIAKAQASRGSDWGQKRKGLFNRIRQVIPMIIPL
                     FITSLRRSENLALAMDARAYGVLKYRSSMTELHFSFRDGLAVLISVVVSLAVFFA"
     gene            complement(8678..10417)
                     /locus_tag="PLW25_05340"
     CDS             complement(8678..10417)
                     /locus_tag="PLW25_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOS53948.1"
                     /translation="MPPLIEFDHVTYRHPGSEHSSPPALNDISVKIHQGEWVAIVGAN
                     GSGKTTFARHCNALLLPSHGQVLIAGQNTALKSNLPKIRAGVGMVFQNPEDQIVATLI
                     EEDTAFGPENLGVLPAEIRARVDAALETVKMSGHKEQLPHLLSAGQKQRVALAGVLAM
                     QPECVIFDESTAMLDPRGRLDVLAEMQSLHNRGITVLFITHFMEEAARAERVLALNAG
                     NLVFDGAPQDLFADLKLLTACGLEQPSTVMLLREIRQLNAQLNHLPVDFEQALAQIPP
                     YRGSHQLLSERLSPMTGEILIDVENLSHTYLPDTPLSHQSLFDVTLQVHSGAAHGFCG
                     ATGSGKSTLLQHLNGLYRPQSGKLRIGPYDFRDPRLDIKAVRRFAGLVFQNPEIYFFE
                     QYVGDEIAYGPKNLYGKENLAKRVREAMQLVGLDFDKFKDRLTYTLSGGEKRKVALAA
                     TMALQPRLLILDEPTAGLDPASRRNLHGILKDYQESGVELVLSSHSMGDITELTENLT
                     ILSKGKALRTGNTADLFNDARLIDEASLGQPSIVKLAGALREKGWPIPQRVVRAEQLF
                     QALRAVNTGGPHE"
     gene            10599..11384
                     /locus_tag="PLW25_05345"
     CDS             10599..11384
                     /locus_tag="PLW25_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS53949.1"
                     /translation="MKRLIYLAVVLVLVFALAACSASSDHLAAIKKAGKMVVGTSADY
                     PPFEFVDEAGNTTGFDVDLMNEIGKRLGVKVEWVDMPFDSLIAAVQGGKIDLSIAAFN
                     YDEERDKTVDFTDAYYTSEDALVIMESLAGDPPTPENLEQFIIGVQSGTTQDAWLTDN
                     LEATGKLKAGSLQRYERVDQAFMDLQAGRVQVIMSDYVPAKALVDKFGGMKIVYQGVL
                     STGPMNIVIPDGDKHLQTEVNKIIKDLQDEGFIDALAVKYFSE"
     gene            11514..12149
                     /locus_tag="PLW25_05350"
     CDS             11514..12149
                     /locus_tag="PLW25_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOS53950.1"
                     /translation="MKFLQYIASGTAYTIAVTLVALPSGLLLGILFSILNVYGGKVTQ
                     RLTSLYSTIMRGIPPIVLLFILYFIVSGSINLSPFWAGSISLGIVSSAYQMEIVRGAI
                     LSVSGGQMMAARAIGMSKNQAIRHVILPQTLRLAIPPWSNEAAIVVKDSSLVYALGVP
                     EILRRAQLISASTQEPFLAYAVAALIYFLLVFITNRLLDALDRRLHIPANP"
     gene            12232..12927
                     /locus_tag="PLW25_05355"
     CDS             12232..12927
                     /locus_tag="PLW25_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOS53951.1"
                     /translation="MDLVVNEGDTIVIIGPSGTGKSTLLRCINLLTIADEGQIWLDDI
                     EITAKSCDEDKVRQHIGMVFQNFLLFNHLTALENVMLGLTKVRHMKPDEAKHKALLEL
                     ERVGLLDRADHYPGQLSGGQQQRVAIARALAMDPRIMLFDEPTSALDPELIGEVLEVM
                     RKLALEGMTMLVVTHEMGFAREVADRIVFLEKGSIVEEGSPEDLFYHPKHERTREFLW
                     KITELYGKKEKES"
     gene            12928..13608
                     /locus_tag="PLW25_05360"
     CDS             12928..13608
                     /locus_tag="PLW25_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOS53952.1"
                     /translation="MIAWLKLFERYLPEFIKGAGVTLELTAVGLTLGFVLGLALAVAR
                     VYAPKWLKTIAIGYIELFRGTPLLVQLFIIYYGLPSLGITLSQTLSAFLALGLNSAAY
                     QAEYLRGAILSIGASQMTAGRAIGLSRWKTIWYVILPQALRLVIPAWSNEPISLLKTT
                     AIVFLIAVQDLMAKAKRAAADTYNPVGSYLAVAVVYLVLVFIFTWGLKYLEKKTRIPG
                     FEMGLKKG"
     assembly_gap    13738..13837
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13838..14343)
                     /locus_tag="PLW25_05365"
     CDS             complement(<13838..14343)
                     /locus_tag="PLW25_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOS53953.1"
                     /translation="MTHPMDLISLQDIKDAHKRIQPYILRTPLIHSVFLSERVNAPVS
                     LKLECLQPTGSFKLRGAANRLLQLTEEEKTRGVITVSSGNHGKALAYMGELLGVPVTI
                     FLSTHTPACKVESLRKTHAHIIVTGNSYDEADAAAIEYEEKNGLVHVSSFDDLRVIAG
                     QGTMALEIL"
     regulatory      14741..14849
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            14876..15532
                     /locus_tag="PLW25_05370"
     CDS             14876..15532
                     /locus_tag="PLW25_05370"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOS53954.1"
                     /translation="MSINHVLVFANGELMDPRAAREMATRADWIIAADGGLAHVQALN
                     LKPDLLVGDLDSVSPEQIRWAEKMGAEVRRFPFDKDETDLELALQEAVTTGCSRITIT
                     GALGGRLDQTLSNIYLLNLPVLADLNVRIDDGAQEVILIHSAVALQGSPGDTVSLLPL
                     SPIVRGISTSGLKYPLTNESLIFYRSRGISNEMLAEIASVQIQSGILLCIHARREVTL
                     "
     gene            15529..>15832
                     /locus_tag="PLW25_05375"
     CDS             15529..>15832
                     /locus_tag="PLW25_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOS53955.1"
                     /translation="MKRRLWVCALLSALLLTAGCTRAVATETAPAVLTVMTHDSFSVS
                     ETVVKTFEEENGAKLVFLKSGDTGAALNRAILTRSHPEADVFYGIDNTYLSRALQEQ"
BASE COUNT         3633 a         3965 c         4460 g         3674 t
ORIGIN      
        1 ataccatgca aaaaaacgaa aactatgata accttaacca gaggttcgaa gaatggctga
       61 aagttcacta agcaacattg acccggtctt cccctgccgt gaatgtcagg cggggcatat
      121 gcaccgtgat tacatcgctt acttcacctg gctgggagat gagctcatct ctgtgcctga
      181 tttccctgcc tgggtgtgtg acatctgcgg gcgatgcgaa tatgatgaaa acgcgctgac
      241 ccgcctgact ttactcttaa gccccaacgc aggaaagacc atcgctcccg cccgccttcc
      301 gcgcacgaaa acgcgcgtta aacccaaagt gcaaagcccc actcaacctg actaaaaact
      361 cccggcacac cgggagttcc ttttagggga gggggaaagg caccggctgc caggcgggtt
      421 ctttatcctg gctggcgagg ttggtaaggt tggtcacctg ctcgccgttt ttatccatga
      481 tgtagatttc tgcattgccg tcgcgcgtgg aactgaacaa cagccactgc ccatcaggcg
      541 accagcacgg ccagctatct ttgccggaac tggtgagcgc ggtgatggtt ttaccacgat
      601 cggcaaatgg tgcgacgtaa atattggttt tactaccgct cgtttgcgtg taggcgagca
      661 gggcgccatc caggctcaga ttgggttgtg aaaccgaacc cagccgtccc tcgtcagaca
      721 tacggtcaaa tttatgttcg ccgttggtat attgactgta tttatcgcgc atatacacaa
      781 cattcaggtt gcctgtcgat tgaacaaaca gcaggaactc cgcgtttggc gtccacgttg
      841 cgtagctttc agcgaaaggc aggctcagcc gggtttgttt ttctccctgt gggtcagtca
      901 tgaataattg ggggatcccg tcatcacgat aataactggt atacacgatc atgtctccta
      961 caggcgacca ggaggggtat ttgtcatcgc gctggcgcaa gctgatatcc actgcttgcg
     1021 catcgggaac tgaaagatcc agcagccaga tatccatagc caatgggttg ccattggcgg
     1081 taaacccttt tgaaaaacca ctatacaaca atttgctgcc atctggagac cacgcggggt
     1141 tggctttgtc tccttcgcta aaggtcagtt ggacgggatt gaccccaacc agttgacctg
     1201 actcgttttg catcacatcc atcagccaaa tctggtcaaa accatctgcc tggcggttgc
     1261 tgacgaatgc cactttgccg cctttaccaa tggaagtgta gatcggcgct tcggtttcgg
     1321 caggcagagg ttcagcggtg gaagttggtt ctatgttctg cgcggcggtc ggttcttcgg
     1381 tggcgggcgc gggcgtctcg ggcgattgcg cggtgatcac aatgaccatg ggcgtaaggg
     1441 ttggaggagg ttcgttcgaa ggagtgctcc gcagagacag gttgcgaaga accaaaacgg
     1501 ccgcgatgcc caaaccaagc actgccagcg cgccgatgat ccaaccgaga ggagatttct
     1561 tttttgctgg cagcgctggc gcaacctgag ccggaggagc aacggatgga gggggtacga
     1621 gcggagcgcc gcgtccaaca gctgcaaccg tgcgctgtcc gaccagggta ttctgagaag
     1681 aagagggatc tgggatggga gaggtattgc aaagagacgc gatgaaatcc tggacagtgg
     1741 aaaaccgcgc ttcaggtttg atggagaggg ctttttcaat ggcagatgcg gtgtgcgcgg
     1801 gaatggtggg attcaattgt gtcagtgggt tcagttcttc ctcgccaatc atgcgcttgg
     1861 cgctgtcagc agggggtttg ccggcgaaca ggtaatacaa tgtggcggcc aggctgaact
     1921 ggtctgaata tggtccggtg cgcaaaccgc tcacctgttc aggtggcgcg taaccaggcg
     1981 tgtaccccca ggcgccggat aaggtttctt gagaggcttc accggtttta gcaatgccaa
     2041 aatccaccag aatcacttca cctttggggg tgatcttgat attggcgggt ttgatatcgc
     2101 ggtggtaaat gggcgggttc tgtgagtgca ggtagctcag cgcattccct agctgctctg
     2161 cccactgcag gatgagcggt gtggaaagcg gtgtcttctg ttccacgatg gtgcgcagat
     2221 tctccccggg gataaaatcc atcaccaggt actggctgtc gttttctgtg aagtagtcga
     2281 tcacgcgcgg aaggttaggg tgtttgagcg aggccagcag acgggcttca cggatgaatt
     2341 gcgcgctggc gttagggctc aggttgtggt tgaccttgac ggcaacctgg tttgccaggc
     2401 tctgatcttc tgcgaggtat acttcgccca tcccgccttt cccgagcagg tcaagaacct
     2461 tgtatcttga tttcaaaatg aaaccgggat cgatatctgt cataagaagg ctttctttgt
     2521 gtgattttta ttttattaat gatacacaaa aaatcaaaaa acgtgccggc agaatcgcgt
     2581 ctttatcggt tcgatgacgg gggtcatact tgctgctcgc aagataatgt tcaaccttag
     2641 taagtttcct gggtgagggg tgaggataag ataaaacccg ggcagccaca cgctggaaaa
     2701 ggcccggggg ttggttgaga ggttgttaca ctgaggatca gtttatttgg cggctggttt
     2761 cttttctttg gatttcttgc gccttaggag atacccgatc acaaaaaatg ccaccccaac
     2821 cagtatccct gccagctgga cgttattgag cccggggtag gtaccaatac cgagggtgtc
     2881 agcgagcagc gacaccgcaa caatgaccaa gccagcgatc ataccaatga gacctgaagt
     2941 ttttctgaac aaggcgtcct ccttacaaat agatatgtat atcatagcac aagtcacaag
     3001 cagcgataat cacaagattg taaactgaaa aacagaaatc ttgtccctgt aatcaaaaag
     3061 aaggcgtctg ctggttcttt tgaaaaaggc tggttgacca ttttcaaaaa ggaagaaccg
     3121 tcatgaggtg cgcgaacgga gaagaaaacc ccctgcaggt tgatggacgc gctggcatgc
     3181 agcgaaccag gagcaccacg agtatttatt aacgtaattc acacgaagaa tgctaaaccg
     3241 cgaccgacct ttccggtgcc tttcagttgc gctcctgggt gaactggcac tgatattgca
     3301 aggattcctc cggaaacaag gtgcctgaat gaacgggtta cctgtacagg tggtgcgata
     3361 tgggtttgaa atacaatgat tttgcgatac gcggaattga cgtcagcgag ttcaacggtg
     3421 cgatcaactg gtccaaggtg cagggacact tcgctgccat tcgcgtgggt catggtcgtg
     3481 ttatagacaa gcgttttacc gcaaactggg caggcgccaa aggcaaggtt gaccgcctgg
     3541 cctactggta tatggattac tacagccata aagacaagtc caccagcgcg tatggcatca
     3601 gcgataaaga ctgggggcgg gtgcaggcgg aaaagtgctg gagcgccctc aaaaatgacc
     3661 cggaaggcat ggtcttttta gatattgaga aatccagtta cggaccggcg ctttcaaccg
     3721 tgcaaccacg ggtgctcgcc atcgcgcaat ctttttttga tcattacgat aaattttcag
     3781 gcaaactcaa cggcatctac tgctcgctca gcatgtgtac ttactttaac gaggcattgc
     3841 gcaaacgccc gctgtgggtg gcgtggtaca acgaggcgca gaacattgat tcagtgaaga
     3901 aggcagtggc tgcccgcggc tggctgggga agtgccttat ctggcaatat gccagcgacg
     3961 gagacgtgaa tgatgatggg aaagccgacg gcaccagcct gggtatggcc tatggcttcc
     4021 tcgacttaaa tggctggctg ggcacgaacg ctgaatatca gcaattattc ggaaagaagg
     4081 ctgaagtgga actctacaaa gtgaaaatat tgatttacaa cctgttggtg agaaatgggc
     4141 cggggctgac ctataaccgc ctgcgccggg ctgattaccc tggagaatac agcatctacg
     4201 aagagagaga cggctacggg cgcatcagtc agacgctctc tgagtggatc agccttgacc
     4261 cggcttatgt gaaaaagtta ggcgctgccg cggttgaact aacggacgct gagaagctgg
     4321 cgcggctatg ggcggctcac cccgaattac actgatgggg gtataagcgt aaacatctca
     4381 cctggattgc ggaaacctgt gagtatgaaa aaagactctt tcgagtctct tagagcgggc
     4441 gatgggactc gaacccacga atatcagctt ggaaggctga cgccttacca cttggcgacg
     4501 cccgcatcac gttcaggatt ataaaccaac cttaatgaat gggcaaggaa aaaaactggg
     4561 agggcgcgcc ctcccagtag gacattatga tacaagctcg cagtagcgtt ctacacgctt
     4621 ctcactcacc ctcaggctgt cttcccagaa cttgcgtgtg cgaatgtcaa taccgaagcg
     4681 cgctgccagg tcggctgcag gggcttcccc ggtgctcgcc agtagatgga tgtaattagg
     4741 cacgaattca gcgccgcgct gctgatagat ggcgtacaac ccggtagcga aaagatgccc
     4801 aaaggcgtag gggaagttat agaatgaaag gccgccagaa tagtagtgtg gcttccatgt
     4861 ccacatccac ttttgcaggt atctttcgtc aagtccatcg ccgtaagtgg ccttctgcgc
     4921 gttctccatt atctcgcata tctcatccgc gttgagctcg gtcttctccc ggcgttcgaa
     4981 tacttccttc tcaaacaggt aacgcgagta gatatccacg atcacctgcg aatcaccgtt
     5041 caactggctt tcaaggatgg ccagctcttg ctgcgggttg gtgacctctt ccaacagcgc
     5101 cgaggtaacg atggtttcgc acatgatgga agcggtttca gccagcgtca tgggagtgaa
     5161 cttctggatt tcggtcttac cggctttgac cgcgcagtca ttgtggaaac catgccccag
     5221 ttcgtgcgcc agtgtggaaa cctggtcaaa tgaaccgtca aaattggaga gaatgcggct
     5281 ttcacccact ccgggcactt ccatgcagaa cgctccgccg cgtttaccgt cacgctgttc
     5341 cgcgtcgatc cagttgtgtt caaaggcgtt gcgggcgaag gcttccagtt ggggggcaaa
     5401 acggccaaaa ttcctgagga tgacctctct ggcttctata aacgagtaat ccgatttgac
     5461 ctcaccgacc ggagcgaaca ggtcccacca ggggagtttt tccttgccca ggtatttggc
     5521 cttggcttta aagtaacgcc ggaacatggg aaaagaatcg cgcatcgcgc ccagcatggc
     5581 ttccagcgat tgccggtcaa tatgcgccgc gtcgatggcg ctgtgcaccg cgtcttcacg
     5641 cccgcggcgg cggttgagca cgtttacttc gcccttgatg ctgttcagag cggcagacag
     5701 agggaccttc agtgaggtcc atagtttgtc ttctgcttcg taaccgcgct tgcgcgtttc
     5761 gccatccggg tgcgaacgaa ggttgatgag cgccggtgag ggcagccttt ttaactcgcc
     5821 gtctacttcg atctcaacac tggcctgcga ggtgagcatg ccctgcagtt ttgtccaggc
     5881 gttggaaccg ctcaggctga gctctgcggc cagcatttct tctgcttcgc tcatcaggta
     5941 gcggctttga cgggcagatt cttccaggaa gtaacgatgt tcttccaaca ctggcacgga
     6001 ctggatcatc tcaggaagac gttcctttac cttgcctatc cagcccagca actgggtttc
     6061 cagctggttg atcttcactg ccaccaggtc aaattctgat tccttacgca aggcttcctg
     6121 gttatatgaa tctgtagaaa cgaatgattc aatataggcg cggatagtac cgccaaccaa
     6181 aaggatagcg ttgtagcatg cgatcatttc ctgcgcgagc gcgccgagcg ttgaagcatc
     6241 ttcctggccg ctgaaagccg ccgctttttc catcaggctt tccatatccg agatgaacga
     6301 tttcaccttt tcgcaatcgg cgttgtattc tttcgaggaa agggaggggt agacgttggt
     6361 caggttccag cgtggtgcgg tttgtgccgt catgtgggct cctttgagtg agaattaaca
     6421 cgttgattat atgccaaaaa cggctagaat taggcaacac gaacaagata aggaaaactt
     6481 ggtgccataa aaattcgtat atgtaatata aggcaacgat gaagaaagga aatgatatga
     6541 gtgaacaatt cttcaaccag gaaagaagag aagtcagcgg tttttcggcg ttggtcttca
     6601 gagggatggg gagaatcgac ctcctccaga gcgatcacga agagttggtg atcgaaacgc
     6661 agcctgaaat acgttcacgc attaaaacgg aggtgcgtga gggaacgttg tacatcgatt
     6721 atgatgaaaa ctggaaagac tggaccggtc tgcgcagcct gagtggagac aagatcgttt
     6781 tcaaactgat gatgcgtgag gtgacggcga tttctatttc cggtgtgggc agcctggaca
     6841 cgccgcgcat tgagagcgcc aatctttcat tggccatcac tgggccgggt ttactgaccg
     6901 ttggaacgtt gaaggtgccc acgctgacgg tgaacctttc gggagttgga gccattgacg
     6961 tcgccggtca gacggatgag ctgagcgtta ccctcagcgg agctggttct ctcaaagcca
     7021 gccggcttga agccgggaaa gccagcgtga ggctcagcgg cgtaggaacg gccaccgtgt
     7081 gggcaaaaaa cagccttgag acttcgatct caggcgccgg ggtcattgaa tattatggca
     7141 gcccgcagat aacgcaaaac aataccggcc tgggtgtgtt aaaattcatg ggcaaccgtt
     7201 agcctgatag gggttgtaaa taacgatttt ggagtattca atggcacaag atagaactcg
     7261 taagattgtt gtcaccgccg tcctcggagc gatcaccatc ctgcttgggt tgacgcactg
     7321 gggttttatt ccatggtttg ggggtatttc cctcaccatc atgcacgtgc cggtcatcat
     7381 cggtgccatt ctggagggtc cgttggtggg agctggtatc ggtctcatct ttggtctgtt
     7441 cagcatgctt caggcggcga tcgcccccac gggtccgctt gaccccctgt ttaccaaccc
     7501 cttgctggct gttttaccgc ggctttttat aggcccggtc gcctggttgg tgtggacagc
     7561 gttgaagaag tggccggtcg tggggttgat cgccagcggt atcgccggca gccttaccaa
     7621 cactgttttg gtgttaagcg tgctggggtt tttctttggt cgcgcgccag tgatgaccca
     7681 gatttttggc gaagagctgt ggaaaccact gagcggaatc gcggtggtat caggcctgcc
     7741 tgaggctggc gtttcagcct tgattaccct gctggtggtg gcggcttacc gccagttcag
     7801 cgtggggaag cgcaaaggcg ccaacttgga ataaaccgcg taaaaaatac ggcagcgatg
     7861 gtctcacgcg aagaagactg cgagtgagac cactacgctt atcaagacgg ccaagccatc
     7921 ccggaatgag aaatgcaact cagtcatact ggagcggtat ttaagcacgc cataagcgcg
     7981 ggcgtccatc gccagcgcca ggttttcaga gcgccgcaga ctggtgataa agagggggat
     8041 gatcatcggg atcacctggc ggatacggtt gaacagccct ttacgttttt gtccccaatc
     8101 tgaaccgcgt gacgcttgcg ctttggcaat acgttctgct gattgcgcca ggaagggcaa
     8161 gaagcgcagt gtcacctgca cgatcatcac aaagtccatg gtttgaatac caacgcggtt
     8221 gagagggctg aggattttcg tcaacccgtg cagcagctca gatgttgagg tgacgaagct
     8281 caccaggctg aggcacagaa tgagcccggc gaaacggatg agcagcatga cccccgccag
     8341 cagaccgccg gaggtgatat tcagttttcc cagcttaatc aggatgttcg ggtctaacgc
     8401 gctggaatag aaaaacacct gaatgatggc gatgatcaaa aggaaaggca aaggtacgag
     8461 caggccctta agcgcaaacc cgatggggac tttggaaagg agcagcgcca gcaggagcgc
     8521 ggcgatacca atcgacagcc cgagttgtga aggggtaaaa gtgatggcga tgagcagcac
     8581 gccaaacatg atgattttcg cgcgcgggtc aagccggtga atgatagaat tcgtagggat
     8641 gtattgaccc aggggaatgc gcgagaggaa atcaaattca ctcatgcgga ccccctgtat
     8701 tcactgcgcg caacgcctgg aataactgct cggcccgcac gacgcgctgc gggattggcc
     8761 atcccttttc ccgtaaggct cctgctaatt tgacaattga cggctgcccc agcgaggctt
     8821 cgtcgatcaa ccgcgcgtca ttaaacaggt cagcggtatt accggtgcgc aacgctttcc
     8881 ctttggaaag gatggtgagg ttttcagtca gttcggtgat atcgcccatg ctgtgcgagc
     8941 tgagcaccag ttccacgccg ctttcctggt aatccttgag gatgccatgc agattgcgcc
     9001 tggacgccgg gtcgaggccg gctgtgggtt cgtccaggat gagcagacgc ggttgcagcg
     9061 ccatggtagc cgccagcgcc accttgcgtt tctcaccgcc actaagcgta taggtaaggc
     9121 ggtctttgaa tttatcgaaa tccagaccca ccagctgcat cgcttctcgc acccgctttg
     9181 ccaggttttc tttgccgtac aaattcttgg gaccgtaagc gatttcatcg cccacatact
     9241 gctcgaagaa gtaaatctca gggttttgga acaccaaccc ggcgaaacgc cgcaccgctt
     9301 tgatatcaag acgcgggtcg cgaaaatcgt acggaccgat gcgcagtttt ccgctttgcg
     9361 gacggtagag cccgttgaga tgttgcagca gggtggattt gcccgaacct gtggcgccgc
     9421 aaaaaccgtg agccgcgccg ctatgtactt gcagcgttac atcgaaaagg gattggtgcg
     9481 aaagcggggt atcaggcaga taagtatggc tgagattctc cacatcgatc aggatttcac
     9541 ctgtcatggg tgaaaggcgc tcagagagaa gctggtgtga accgcggtag ggtggtattt
     9601 gcgccagcgc ctgttcaaaa tcaaccggca aatggttcag ttgagcgtta agctggcgaa
     9661 tttcgcgcag cagcatcacg gttgagggtt gctccagccc gcaggcagta agcagcttca
     9721 ggtcagcgaa aaggtcctgg ggggcaccgt caaacaccag gttgccggca ttaagcgcta
     9781 aaacgcgctc ggcgcgcgcg gcttcttcca taaagtgggt gatgaacagc acggtgatgc
     9841 cgcggttgtg cagcgattgc atttcagcca gcacatccag cctgccgcgc ggatcgagca
     9901 tagcggtgga ctcgtcgaag atgacgcatt ccggctgcat ggccagcacg ccagccagcg
     9961 ccacacgctg tttttgacca gccgagagca ggtgcgggag ttgttcttta tgcccgctca
    10021 tttttacggt ttcgagagcc gcgtccaccc gcgcgcgaat ttccgcaggc agcacgccca
    10081 ggttttcagg tccgaaagcg gtatcttctt cgatcagcgt ggcaacgatc tggtcttcgg
    10141 ggttctgaaa gaccatccct acccccgcgc gaatcttagg gaggttgctc ttcagcgcgg
    10201 tgttttgccc ggcgataagc acctgtccgt gactgggcag cagcaacgcg ttgcaatggc
    10261 gcgcgaaggt ggtcttgccc gagccgttgg cgcccacgat ggcaacccat tcaccctggt
    10321 gaatttttac gctgatatcg ttcaaggctg gcggggagga atgttcacta ccgggatggc
    10381 ggtaggtgac gtggtcaaac tcgatcaaag gcggcaaaac gcgctccctg aaaaagtgat
    10441 acctgcaaac acgcggttat tatactggaa gaaaaccctt acacttgaag atatttcccc
    10501 ccatttttgc ttgacttcct gttcagttgt gtattcttat tcctgttata taataagtgt
    10561 aaggtttcaa cgtagaataa atttaaacgg aggaaataat gaaacgcctg atctaccttg
    10621 ctgtggtgct ggttctggtt tttgctcttg ctgcgtgcag cgcatcttcc gatcacctgg
    10681 cggcaatcaa aaaagctggc aagatggtgg taggaacgtc tgctgactat ccgccttttg
    10741 aatttgtgga tgaggcgggg aataccaccg gttttgacgt ggatttgatg aatgagattg
    10801 ggaaacgcct gggtgtgaag gtggaatggg tagatatgcc cttcgatagc ctgatcgccg
    10861 cagtacaggg cggtaagatc gacctttcca ttgcggcttt caattatgat gaagaacgtg
    10921 acaagacagt tgatttcacc gatgcttact acaccagtga agacgcgttg gtaatcatgg
    10981 aatcactcgc aggcgaccca ccaactcctg aaaacctcga gcagttcatc atcggggttc
    11041 aatcaggtac tacccaggat gcctggttga cagacaacct cgaagccacc ggaaagctga
    11101 aagcgggttc cttacagcgt tatgagcgtg tggaccaggc ctttatggac ctgcaggctg
    11161 gccgggtgca ggtgatcatg tctgattatg tgccagccaa agcactggtt gataagttcg
    11221 gcgggatgaa aatcgtctac cagggtgtgc tttcgactgg tccgatgaac attgtcattc
    11281 ccgatggcga taagcacctg caaactgagg tcaacaaaat catcaaagac ctgcaggatg
    11341 aagggttcat cgacgcgctg gctgtcaagt atttctctga gtaattttct cggcattcaa
    11401 acggaggtaa cggtatcagt gacctgatta ccgttacctc gaccacaacc gctgcgcttg
    11461 ttcacttata gcgctgccta tcctcaacca ggagtgattg agcgttggat ttcatgaagt
    11521 tcctgcagta tattgcttcg ggcaccgctt atacaatcgc agtcacgttg gtagcgttgc
    11581 cttccgggtt gctgctggga atcctcttct caattctgaa cgtttacggg ggaaaagtta
    11641 ctcagcgcct gacctccctc tacagcacca tcatgcgtgg tatcccgccg attgtgctgc
    11701 tctttattct ctatttcatc gtgtctggca gtatcaacct ttcaccgttc tgggcgggtt
    11761 cgatttcgct gggaattgtc agcagcgcct accagatgga gattgtgcgc ggcgcgatac
    11821 tatctgtcag tggcggacag atgatggctg cccgggctat tggcatgagc aaaaaccagg
    11881 ccatcaggca tgtcattttg ccgcaaaccc tgcgcctggc catcccgccc tggtcaaacg
    11941 aggctgccat cgtggttaag gattcttcac tggtatacgc tttgggcgta ccagaaatcc
    12001 tgcggcgcgc tcagctgatc agtgccagca cccaggaacc tttcttggcc tatgcggtgg
    12061 ctgcgctcat ctatttcttg ctggtgttca tcaccaaccg tttgctggac gcgctggacc
    12121 gacggttgca tattccagcc aacccatgat ttggagacag acatggcaaa accgattctg
    12181 accatcaaga agctgaccaa aaaatacggg gaaaacgtca ttttccatga tatggacctg
    12241 gtggtgaacg aaggcgatac catcgtcatt attggcccca gtggaacagg caagagcacg
    12301 ctgctgcgct gtattaactt gctcacaatt gcggatgagg gccagatctg gctggatgat
    12361 atagagatta ccgccaaaag ttgtgatgaa gacaaggtgc gccagcatat tggaatggtg
    12421 ttccagaatt ttctgctttt caaccacctc accgcgttgg aaaacgtgat gctgggtctg
    12481 accaaggtca ggcacatgaa accggatgaa gccaaacaca aagcgttgct cgaactggag
    12541 cgggtagggc ttttagaccg ggctgaccac tacccggggc agctctcggg tgggcagcaa
    12601 cagcgcgtgg cgattgcccg cgcgctggcg atggacccgc gcattatgct tttcgatgaa
    12661 ccgacctcgg cgttagaccc tgaattgatt ggtgaagtgt tggaagtgat gcgcaaactg
    12721 gcgctcgaag gcatgaccat gctggtggtg acccacgaga tggggtttgc cagagaggtg
    12781 gctgaccgca tcgttttcct tgaaaaaggc agtattgttg aagaaggttc accagaagac
    12841 ctgttttacc accccaagca cgagcgcacg cgtgaatttc tctggaagat caccgaactg
    12901 tatgggaaga aagaaaaaga gagttaaatg atcgcctggt taaagttgtt tgagcgctat
    12961 ttacccgaat tcatcaaggg tgcgggggtc acccttgagc tgaccgcggt tggactcacg
    13021 ctggggtttg ttctcggtct cgcgctggca gtcgccaggg tatatgcacc gaaatggttg
    13081 aaaaccattg ccatcggtta cattgagctc ttccggggta cgccgctgct ggtgcaattg
    13141 ttcatcatct attacgggtt gccttcgctg gggattactc tatcgcaaac cctttccgca
    13201 tttctggcct tgggattgaa tagcgcggct taccaggcag aatatttgcg cggcgcgatt
    13261 ttatctattg gggcgtcgca gatgaccgct ggcagagcga ttgggctttc gcgctggaag
    13321 accatctggt atgtcatcct gccgcaggcg ctgcggttgg tgatccccgc ctggtccaac
    13381 gagccgattt cgctgctgaa aaccacggcg attgtgttcc tcatcgcggt gcaagacctg
    13441 atggcaaagg ccaaacgtgc cgctgcggat acctacaacc cggtaggcag ttacctggcg
    13501 gtcgcggtgg tttacctggt attggtgttc atattcacct gggggttgaa gtacctggaa
    13561 aagaagaccc gcatcccggg ttttgagatg ggattaaaaa agggctgagt aacgctcgac
    13621 cagttccata aacgtgggta gattaatatt gctgccactg atgaccactg tggttggggc
    13681 atctggcttg attttgcctg ccagcaaggc ggcgaccccc accgcgcccg aaccctcnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaga atttccagcg ccatggttcc
    13861 ctgcccggca ataacccgca gatcgtcgaa tgaagagacg tgcacaaggc catttttttc
    13921 ttcgtattca atcgctgcgg catcggcctc atcataacta ttgcccgtta cgataatatg
    13981 cgcgtgcgtc ttgcgcagac tctctacttt gcaggcgggt gtatgggtgg atagaaaaat
    14041 ggtgaccggg actcccagta attcacccat gtacgccagc gcctttccat gattgccgct
    14101 ggaaaccgtg attacgcccc gcgttttctc ttcttcggtg agttgcagca agcggttggc
    14161 agccccgcgc agcttgaatg aacccgttgg ttgcaaacac tcaagcttga gtgagaccgg
    14221 ggcgttgact ctttcgctca gaaaaactga atggatcaaa ggtgtgcgca ggatgtaagg
    14281 ttgaatccgt ttatgcgcgt ctttaatatc ttgaagcgag atgagatcca ttggatgcgt
    14341 catagaacct atgacttatt ttatatcaaa tagcaagaag gacctgtaaa cttaattgag
    14401 gaaaatcctg ctcagggata attattaggg acaacccccg agtaaggcgc gaaagcctga
    14461 cttctcgcat aagcaggagg aaccaatcct ttgaaggagc caaacgcgaa tgacggtttc
    14521 ttacgatacg gaggacaaaa atcgttgatt ttgatgggta ccagataatt ccttttctgg
    14581 gctattcagg aaacggtttt acaggccagg taaagggttg gcagcggaag gatttgaacc
    14641 ccgtgatagc ggcaggcagc ctgcagcgcc tttggcagcc aatgctggcc ctcacatcgc
    14701 agccttgaca ttcatccttc acccgtctat actcaatgta gataaagcaa cggggagccc
    14761 gctgcacggg ctgagagtag ggagcgaacc ctagacccgc attacctgat ccggattatg
    14821 ccggcggagg gataatcact caaccataca accctccctg gcggagggtt gtttaatgtc
    14881 aattaaccac gtgcttgttt ttgccaatgg tgaactgatg gacccgcgag cagcccgtga
    14941 gatggcgact cgcgcggatt ggatcatcgc ggctgatggc gggctggctc acgtgcaggc
    15001 gctcaacctc aaacctgacc tgttggtcgg ggacctggac tcggtatccc ctgagcagat
    15061 tcgctgggca gaaaagatgg gcgctgaagt gcgcaggttc ccttttgaca aagatgaaac
    15121 cgatctcgaa ctggcgctgc aagaggctgt cacaacaggg tgcagccgca ttaccatcac
    15181 cggggcgctt ggcggaaggc tcgaccagac gctctcgaat atctacctgc tcaacctgcc
    15241 agtgctggct gaccttaatg tgcgcatcga tgacggcgcg caggaggtga tcctcattca
    15301 tagcgcggtg gcgcttcagg gcagcccggg tgacaccgtg tccttgctgc cgctctcgcc
    15361 aatcgttcgc ggtatttcca ccagcgggct gaagtacccg ctgaccaacg aaagcctgat
    15421 tttttaccgc agccgcggga ttagcaatga aatgctggct gaaatcgcca gtgttcaaat
    15481 tcaatcaggc atcctcttgt gcatccatgc gcggcgcgag gtgaccctat gaaacgccgg
    15541 ttatgggtat gcgctctgct tagcgcgctg ctgctgaccg ccggttgcac gcgggcggtt
    15601 gcaacagaaa cagcaccggc ggtccttacg gtgatgacgc acgattcttt ttcagtctct
    15661 gaaacggtgg ttaaaacgtt tgaagaagag aatggcgcta aactggtgtt cctaaaatct
    15721 ggcgataccg gcgctgcgct taaccgcgcg attctcaccc gaagccatcc tgaagccgat
    15781 gtgttctatg gaattgacaa tacttacctc tcgcgcgccc tgcaggagca aa
//
LOCUS       DAOSPM010000044        15133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_3958_length_15133_cov_15.573286, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000044 DAOSPM010000000
VERSION     DAOSPM010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15133
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3958_length_15133_cov_15.573286"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLW25_05380"
     rRNA            complement(<1..318)
                     /locus_tag="PLW25_05380"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            883..>2642
                     /locus_tag="PLW25_05385"
     CDS             883..>2642
                     /locus_tag="PLW25_05385"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOS53956.1"
                     /translation="MTRTRAHLSPSLWLQLGTSLWIGLAATLALLLISHILPLLVFGN
                     RIFPGVSLSGLNLSGLNANEAAALISRSAYYPASGRITLEYGSQQWALSPSQLGVMLD
                     PQATAITAYKIGRSGPLEKVLLDRFNLLRGSAEVQPTFIFNEKATVDFLNAIAAEINQ
                     PLQEASLEIQGVDVFVRNGQPGRVLDVAASLARIAAQVEQVRDGVVPLAVIETQPLIS
                     DVSEQAELAREILSQPLRFTLPAEVGEIEGNWMLEPTALAKMLTFERVQDGGTDSLAV
                     VVNPTLAGLHLDSLRESLDAEPQNARFIFNDETGLLDLKDSAVIGRKLNIQSSLENIN
                     SSLKAGSHEAQLVFDITPPAVTDSMTGAELGITELVYEYTSYFYGSSPDRVQNIKTAS
                     SSFHGLLVPPGGVLSMADELGDISLDNGYAEALIILGDETIKGVGGGVCQVSTTLFRT
                     AYFAGYPILERHAHAYRVGYYEQTPSGGHNANLAGLDATVFVPLVDFKFKNDSDHWLL
                     METYVYNNTALTWKFYSTKDGRQVEHSTTGPTQKVKAPDDLYRENPELKKGEVKQVDW
                     AAEGARVVVTRRVTRGGELL"
     assembly_gap    2643..2742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2963..3187
                     /locus_tag="PLW25_05390"
     CDS             2963..3187
                     /locus_tag="PLW25_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOS53957.1"
                     /translation="MKVNQELLEILRCPACVKEKPGLLEQVREVWLVCTDCGRKYPIV
                     EEIPVLLISEGERWRALPVEDLPVPPPRPE"
     gene            3276..4727
                     /locus_tag="PLW25_05395"
     CDS             3276..4727
                     /locus_tag="PLW25_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOS53958.1"
                     /translation="MAQMTKKPMAHSIRRNLKMDTTTRVLLISFAVVGLLLAFLAGKF
                     IYNTVKSWSITSLPGAPVSSPGSSATTGQIPDLNIQSNTGPEAKSWDGKSRVNILFLG
                     LDATDQRELMEPGPRFSDTMILVTIDPLSQTLGALSIRRDLWVNVPGYDYHKINKAHF
                     IGEAYHLPGGGAGLAVATVEEFLGIPIHYYAKVDFNTFVRVIDEIKGVKIEVKEPILA
                     DWGANGNNFWLEPGVYTLPGTYALAYARYRGSDAGDIDRGERQMQVMMAIRDRILDFK
                     MLPQLIQSAPALYQDVSDGVQTNMSFDQAVQLMVLISKIPRENFKTYNITYDDAAPEM
                     ITTLDEGTQYILRPFPDKIRELRDRVFASEGVAAAPIVMQSGGDPLALAQAEGARISI
                     FNGTSTNGLAEATRDYLTSQGLHVIEASSASEAYSYTTLVVHSATPYTLAYLSSLMNN
                     IPSTRIFNQYDPQSTTDITVFLGNDWATANPMP"
     gene            complement(4781..5617)
                     /locus_tag="PLW25_05400"
     CDS             complement(4781..5617)
                     /locus_tag="PLW25_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53959.1"
                     /translation="MKEKRGSLYLLTGLVIGAVLGLLTSIFIVPARYVDVAPSALKAV
                     DREQYRALVARAYLVEADNGRAVARLALLADPRPADALVAQAEKEKRPGGDEHTSRAL
                     ALLAAAVSQKQLLITPLPRIAAEAGGQATEPAPGEQETPTLPPPTRTPGATITPRPSP
                     TPLPTQGAPYALIQQKEICEVEGRGSLIQVFVFNAADEGVPAVRVEVSITDGGVESFF
                     TGFYPEISPGYADYAMVEGMTYNLRVGSTGQLVQNLSVPQCKNKDGEAFPGSIQLVFK
                     QP"
     gene            complement(5614..5967)
                     /locus_tag="PLW25_05405"
     CDS             complement(5614..5967)
                     /locus_tag="PLW25_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53960.1"
                     /translation="MRKGRLIAFILAIMLGATAGLMYGWLYYPAKISAAAMKSLRADY
                     KADYVLMVAELFATDGDLEKATDRLEKIKPADAIGAVRRGLLTAQEMGYADWEMRLIA
                     DLEIALQQTRQETRP"
     gene            complement(5968..7629)
                     /gene="hutU"
                     /locus_tag="PLW25_05410"
     CDS             complement(5968..7629)
                     /gene="hutU"
                     /locus_tag="PLW25_05410"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF003820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOS53961.1"
                     /translation="MVETRVIRAPRGSTLTCKNWQIEAAYRMIQNNLDPEVAERPQDL
                     VVYGGRGQAARNWESFDAILRSLRELEPDETLLVQSGKPVVVFKSHADAPRVLIANSN
                     LVPHWATQQHFDQLAAKGLIMFGQMTAGSWIYIGTQGILQGTYETLGSLARQKGWQSL
                     RGRFVLTAGLGGMGGAQPLAITMNEGVGLCVEVDPQRAQRRLETGYVDVVVDSLEEAM
                     TLVEEFCQREEPRSIALIGNAAEIYPELVLRGVVPDVVTDQTPAHDVMSYIPAGLSLE
                     EAERMRSDDPRQYEHRSLDSMSAHIRAMLHFQRKGAEVFDYGNNLRQRAFDNGVKDAF
                     QFPGFVPAYIRPLFCEGKGPFRWVALSGDPEDIYRTDEAISKLFPRDEHLKRWLTMAR
                     ERVHFQGLPSRICWLGYGERARAGLLFNEMVARGQLKAPIVIGRDHLDAGSVASPNRE
                     TEGMQDGSDAISDWPILNALVNAAGGATWVSFHHGGGVGIGFSQHAGMVIVADGTPQA
                     AKRLERVLTTDPGMGVVRHADAGYPEAIMAARRLGIHMPMLPKDQ"
     gene            complement(7737..7916)
                     /locus_tag="PLW25_05415"
     CDS             complement(7737..7916)
                     /locus_tag="PLW25_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53962.1"
                     /translation="MGEHCTRATFKALEEPGRVAEFLAYRLKKRPLLTRLVLRQGGLR
                     GQISPPVLLAYARQI"
     gene            complement(8162..9490)
                     /locus_tag="PLW25_05420"
     CDS             complement(8162..9490)
                     /locus_tag="PLW25_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS53963.1"
                     /translation="MRFIDLVSLILYNLGRRKGRVLLTAVGVVIGTAAVVVLVSLAVG
                     LQKSATQQLWGISDLTSIEVYPGYQESRVMGGGGGGGVMYAGGGSDMKLLTPAAIKEI
                     SAIPGVRKTIIHDYLQSGSEVHFGKLMSWGGIIGVNIDDLADMGLKAARGTTELSRGT
                     MVIGSWVPQNFYNPNPRPGEEMTGTPELLDQVIRLVLVKYTQDGEEVRRTFNVRVVGI
                     LEETRGMADGNMYMRLDEVTAFSEWGRGTRINRDKEGYGMLIVKAEDPDVVLDIADQI
                     NALGYMANTPQSYLEGINSFFLVLQIIFGGVGAIALLVAAIGIANTMAMAILERTREI
                     GLMKAVGATNRDVLSIFLGEAAGIGFVGGLGGVALGWGASAILNVVALSYFASQAANG
                     GGSLPTAATSTPFWLPVFALVFATVIGLLSGLYPALRAASLVPVNALKYE"
     gene            complement(9500..11263)
                     /locus_tag="PLW25_05425"
     CDS             complement(9500..11263)
                     /locus_tag="PLW25_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53964.1"
                     /translation="MIKKNRNVLGLLLTLTFLFGMTTLNTYAAPLNDEVPVDSKSKSG
                     LPGSVITYKLDIPGEAGQIMNVSANSTSGWNSPTVVPNTITFGANETKTVSVNVPIPV
                     GATEGQSDITTVDVTVVAGNPVEFDPLQLTTTVVVPKPAIGRPMVTITSYSTGNKTLF
                     AGNEMNLQVVLGNNGKVSAENIIVTFDGTDFYPRESGGVRTTGTLPPGNTNTITQKFL
                     IGDALAWANVASMKATVNYTDPLGTAYSEVFTLSFVIAEPSSSGFAATATPNIPQRPQ
                     LVVTGYSTDIDPLQPGSIFELKLDVKNLGMADAKNVSAVIGGGGMPSNEEGKPQPGGV
                     SGGSSDLTTFAPLGSSNVIFMGDVSKDQTAVVTQKLVVNVTAQPGAYPLKLSFVYIDA
                     KGNRLVDDQVITLLVYSLPQVEVSFYRDAGIITAGMENILPLQVTNLGKKTYVLGNMK
                     VSAENADLLNNILLVGALDPGGYYTLDASLTPYQEGPLDLKVVINYTDDFNQPRFIEQ
                     VIPVEVQPAMEVPPDMGDGNTPNGEGPVVPAPETFWQKVVRFFKGLFGLDSGLQQPVE
                     TYPVQETPSGEVIPVPAIPKG"
     gene            complement(11260..12003)
                     /locus_tag="PLW25_05430"
     CDS             complement(11260..12003)
                     /locus_tag="PLW25_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS53965.1"
                     /translation="MLDKALVRTRDLTKRYTMGENTVVALDGVSLEIDPGSFTVVMGP
                     SGSGKSTLLYLLGGLDWPTSGSITIGEDEIEKMDENLLAEYRRNRVGFIFQSFNLIPS
                     MPAEENVSFPLRFARIPRAQRHARAQEILQKVGLSDRATHKPTELSGGQQQRVAVARA
                     LINDPPLILADEPTGNLDSQSGFTIMKLLSRLHQAGKTVIVVTHDPRMRQFATNMLYL
                     LDGKVVSEEAYRKATENMTQPIVEQEEIL"
     gene            complement(12021..12800)
                     /locus_tag="PLW25_05435"
     CDS             complement(12021..12800)
                     /locus_tag="PLW25_05435"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOS53966.1"
                     /translation="MALNAKGQQTNKHSWNTKWIPSLFTRPARTTSEIVTQEKAAWLT
                     PLLILSVLVILAVAIGAPIKRNVIQMGSELPPDFQYYTPEMQEQFFAAQAKQTSPLFL
                     YVFPLISGLLKVWMPWFLLSILMYLSLTLAGSRAGSIKSYNLVAWSMLPMAVRLLIQI
                     LAMIVSKTVVSAPGLSGFIAAEATGVGAYLRSMLGFIDIYFLFQIALLLLGSVPLSGL
                     SRSKAWTATAISVVIVLLLMAIPGLLASALGGLSLRGGFYF"
     gene            12915..13622
                     /locus_tag="PLW25_05440"
     CDS             12915..13622
                     /locus_tag="PLW25_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOS53967.1"
                     /translation="MRINIGVENNYEGRSLAWMLDYPGCFAYGAEGSEAILRAPQALL
                     RYKEWVDSHLKDSWMKSLGDFDIHLEESYDVFSINDDFDLADEGIEVNAWFRHDWKPL
                     TAEDIRRGTLVLTWSREDLLELVSGLTPAQLDCRFSGERWSIAGVLEHIANAEHWYLH
                     RLGLAVGTRQELPADVFERLKVVRRRLVEVLPSLQGSRQVLGVDGEFWSPRKVLRRSA
                     WHEVDHIEHIFKLISRL"
     gene            complement(13949..15031)
                     /gene="amrS"
                     /locus_tag="PLW25_05445"
     CDS             complement(13949..15031)
                     /gene="amrS"
                     /locus_tag="PLW25_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOS53968.1"
                     /translation="MNPHELLDVLTQPAELVETLEQGNLRCLACAHRCLLKPGRRGIC
                     GVRFNANGVLMAPKGYVAGVQIDPLEKKPFNHFLPGSEVLTFGMLGCNFHCGFCQNWL
                     SSQALRDPEAGRSAYAIRRVSAEKLVTYAVENGARAIASSYNEPLITTEWAVEVFKLA
                     KDKGLKTAYVSNGYATAEVLDYLVPWLDGYKVDLKSMRDSEYRKMGGRLQPVLDTIRR
                     IKELGLWLEVVTLVIPGMNDSSDELWEAGRFLASISPDIPWHVTAYHPDYKENAPPTP
                     AETLQRAAEIGQEAGLRFVYAGNLPGRVGSLENTICPQCGRAVIERRGYLLHGYHINS
                     AGACAFCGQPIAGVWSGRPQAGHANG"
BASE COUNT         3303 a         4413 c         3979 g         3338 t
ORIGIN      
        1 gttccattgt ggggggccac cctctcaggt cccctacccg tcatcgcctt ggtaggctat
       61 taccctacca actagctgat gggacgcagg cccctcccga agcgaataaa tccgtttagt
      121 cgtaaggcat ctaatccaaa cgaccacatg gggtattagc aatcctttcg gactgttatc
      181 cccctcttcg gggcaggtca ccaacgcgtt actcacccgt tcgccactaa gacagtattg
      241 ctacagtctc cgttcgactt gcatgtatta agcacgccgc cagcgttcat cctgagccag
      301 gatcaaactc tccgccaaat aacacctttc ggtgtaagtt acggatctgg tttgttactc
      361 ttttgaaaac gacagggcat cgttttccta tcactcttca gttgttaagg tactgatttc
      421 caagggccag atttttctct cgcgaccgcc gctcgttaag accaggtgac aaaattaccg
      481 atgctccttt acaggacgtc atcggcctgc ctttacgatt tgcttgtgga caagtcctca
      541 agcttgctgc gtttgttggg ttgttacgga tctgcttatt ccagcgaaat cttcaacgca
      601 cttgggacag atattgtaac cgcctgtctt ttccctgtca agggtgtgtt caacgagtgg
      661 gtcacttctc gagtctggtt attgggtttt caatgatctg ctctttaggc gagaccgttt
      721 gccgtgttat gggcaacgag ggatgattat acctgctgat gtgtttgtgt caagggttaa
      781 tcagcaccaa tttcacctct ttttcaactt ccaaggggaa cttttctttc cttttgtgcg
      841 tttgaagaat agggcgaaac aggagtgtta taatctgaat tcatgaccag gacacgcgcg
      901 cacctttcac cttccctgtg gctgcagctc ggcacctcct tatggattgg tcttgccgcg
      961 accctggcgt tgctgctcat ttcccacatt ctccccttac tggtctttgg caaccgtatt
     1021 ttccctggcg tttcactttc cggtttgaac ctttcagggc tgaacgccaa cgaagccgcc
     1081 gcactcatca gccgttcagc atactaccct gcatcagggc gcatcacgct ggaatacggt
     1141 tcgcaacaat gggcgcttag cccttcccaa ctgggggtga tgctcgaccc ccaggctaca
     1201 gccatcaccg cttataaaat cgggcgcagc ggccctctgg aaaaagtgct gctggaccgt
     1261 tttaacctcc ttcgtggcag cgcggaggta cagccaacct tcatcttcaa cgaaaaagcc
     1321 accgttgact tccttaacgc tatcgccgcg gaaatcaacc agccactgca ggaagccagc
     1381 cttgaaattc aaggggttga cgtctttgtg cgcaacggtc aacccgggcg cgtactcgat
     1441 gttgccgcca gcctggcgcg catcgccgcc caggtcgaac aggtgcgcga tggcgttgta
     1501 cctctggcgg tgatcgagac ccagccgctt atctcagatg tcagcgagca ggctgaactc
     1561 gcccgcgaaa tcctcagcca gccattgcgc tttaccctgc cggcggaggt cggtgagata
     1621 gaaggaaact ggatgcttga gccgaccgcg ctggcaaaga tgctcacctt tgagcgcgta
     1681 caggatggcg gtactgattc actcgccgtg gtcgtcaacc ctaccctggc agggttgcac
     1741 ctggattccc tgcgcgagtc gcttgacgct gaaccgcaaa acgcgcgctt tatcttcaac
     1801 gatgaaaccg gtctgctaga cctgaaggat tcggcggtaa tcgggcgcaa gctcaacatt
     1861 cagtcatcgc tggagaatat caatagcagc ctcaaagccg ggagccatga agcccagctc
     1921 gtctttgata tcaccccgcc cgctgtcacc gattcaatga ccggagcgga gctggggatc
     1981 accgagctgg tttacgaata tacgtcttac ttctatggtt cctctccaga ccgcgtgcaa
     2041 aatatcaaaa ccgcctcttc ctcattccat ggtctgctgg tgccgcccgg aggtgtcctt
     2101 tccatggctg atgaattggg tgatatcagc ctggataacg gttacgctga agccctcatc
     2161 atcctgggtg atgaaaccat caaaggcgta ggcggcggtg tttgccaggt gagcaccacc
     2221 ctgttccgca cagcttactt cgccgggtac cctatcctag aacgccacgc gcacgcctac
     2281 cgcgtggggt attacgaaca gacccccagt ggtgggcata acgccaacct cgccggtctt
     2341 gatgccacgg tttttgtgcc gctggtagat tttaaattta agaacgacag cgaccactgg
     2401 ctgttgatgg aaacttacgt gtacaacaac accgcgctca cctggaagtt ctactcgacc
     2461 aaagacggcc gccaggtgga gcacagcact accggaccta cccaaaaagt gaaagccccc
     2521 gatgaccttt atcgtgaaaa ccctgaacta aaaaaaggcg aggtcaaaca ggtggattgg
     2581 gccgcggaag gcgccagggt ggtggtaacc cgccgggtca cacgcggcgg cgagctgctg
     2641 tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaggcgcc agggtggtgg
     2761 taacccgccg ggtcacacgc ggcggcgagc tgctgtataa cgacacattc accacggtgt
     2821 atgagccctg gcgcaacatc tttgaatacg gcccgggcac tgaggacatg ccccccgaca
     2881 agaaaagcgg cagcgcggaa gacgaagaat aaccccccag tataatggta caattgcccc
     2941 gtaatgaaaa agcggaggat gaatgaaggt caaccaagaa ctgcttgaga tcctgcgctg
     3001 cccggcctgc gttaaagaaa aacccggttt gctcgaacag gtacgcgagg tgtggctggt
     3061 atgcacagac tgcggccgta aatacccgat agtagaagag ataccggtgc tgttgatttc
     3121 ggaaggcgag cgctggcgcg ccctgccagt ggaggacctg cccgttcccc cgccaaggcc
     3181 tgaatgattt tttctcccag ctccaattat ctggtattgt tcttgcaaca aagcaccggt
     3241 aacacactaa cctcattaat gatggtgaac tcttaatggc tcaaatgaca aaaaaaccca
     3301 tggcgcactc aattcgccgc aacctcaaaa tggataccac cacccgggtg ttattgatct
     3361 cattcgccgt ggtcggcctg ctgctggcat ttttagcggg taaattcatt tataacacgg
     3421 taaaaagctg gtcgatcacc tctctgcctg gcgcgccggt tagttctcct ggcagcagcg
     3481 caaccacagg gcagattcct gatttgaata tccaatccaa caccgggcca gaagccaaat
     3541 catgggacgg taaaagccgc gtgaatattc tgttcctcgg gctggacgcc accgaccagc
     3601 gcgagctgat ggagccggga cctcgttttt cagacaccat gatcctggta accatcgacc
     3661 cgctctcgca aaccctcggc gcgctctcca tccgccgtga cctgtgggtg aatgtcccgg
     3721 gttacgacta tcacaagatc aacaaggcgc actttatcgg tgaagcctat cacctccccg
     3781 gcggcggcgc cggtctggct gttgccacag tcgaggaatt tctcggtatc cccatccatt
     3841 attacgccaa agtggatttc aacaccttcg tcagggtgat cgatgaaatt aagggggtaa
     3901 agatagaggt caaggaaccc atcctggcag attggggcgc gaacggcaat aacttctggt
     3961 tggaacccgg tgtgtacacc ctgcccggca cctacgcgct ggcctacgcg cgctaccgcg
     4021 gcagtgatgc cggcgatatt gaccgcggtg aacgccagat gcaggtgatg atggccattc
     4081 gcgaccgtat cctcgatttc aagatgcttc cgcagctcat ccaaagcgcg cccgccctat
     4141 accaggatgt ttctgatggc gtgcaaacca acatgtcctt cgatcaggca gtgcagctga
     4201 tggtattgat aagtaaaatc ccgcgcgaga attttaagac ctacaacatc acttacgatg
     4261 acgccgcgcc agagatgatt accaccctgg acgaaggcac ccagtacatc ctgcgtccct
     4321 tccctgataa gatacgcgag ctgcgagacc gggttttcgc ctcagaaggc gtggcagccg
     4381 ctcctatcgt catgcagagt ggtggagacc cactggcgtt ggcccaggct gaaggcgccc
     4441 gtatcagcat cttcaacggt accagcacca acggcttggc agaagccact agggattatc
     4501 ttacttcaca gggcttgcac gttattgaag cgtcctctgc cagtgaggct tattcttata
     4561 ccacgctggt ggtacacagc gccacaccat acacactggc ttacctgtct tcgctgatga
     4621 acaatattcc cagcacgcgc atttttaacc agtacgaccc tcaaagcacg acggatatca
     4681 ccgttttcct ggggaacgat tgggctaccg ccaacccgat gccgtaaacg cagcggttgt
     4741 tgcgcaatct catcaggccg ggcaaccggc ctgatttatt ttatggttgc ttaaaaacaa
     4801 gttgaatgga acccgggaac gcttctccat ccttgttctt gcattgtgga acagaaaggt
     4861 tttgcaccaa ctgccccgtg ctgccaaccc gcaggttata ggtcatcccc tccaccatcg
     4921 cataatcagc atagcccggg ctgatttcag ggtagaaccc ggtgaaaaaa ctttcaacgc
     4981 cgccatcggt gatggaaacc tccacgcgca ccgccggtac cccttcatct gctgcgttaa
     5041 acacaaaaac ctggatcaac gacccgcgtc cctccacttc gcaaatctcc ttttgttgta
     5101 tcaacgcgta aggagcgccc tgtgtgggca agggggtggg gctggggcgt ggggtgatcg
     5161 tggcgccggg tgtgcgggtt ggagggggaa gcgtgggcgt ctcctgctca ccaggggctg
     5221 gttcggttgc ctgcccgcca gcttctgcgg caatgcgcgg caatggcgtg atcagcagct
     5281 gtttttggct taccgccgcg gccagcagcg ccaacgcccg gctggtatgc tcatctccgc
     5341 cgggtctctt ctctttttca gcctgcgcca ccagggcatc cgccggcctg gggtccgcca
     5401 gcagcgccag ccgtgcaacg gcgcgaccat tatctgcttc caccaggtac gcccgggcga
     5461 cgagcgcgcg gtactgttca cgatcgaccg ccttcaacgc cgatggggct acatccacat
     5521 atcgagcagg aacaataaaa atagatgtga gtaaacccag gacggcgcca atgacaagcc
     5581 cggtgagcag gtacaaggac ccgcgctttt ccttcatggc ctggtctcct gcctggtttg
     5641 ttgcagggcg atttccaggt cggcaatcag gcgcatttcc cagtcggcgt agcccatttc
     5701 ctgggcggtc agcaatccgc gccgaaccgc tccaatcgcg tcagccggtt taatcttctc
     5761 cagacggtcg gtagccttct ccaggtctcc atcggtggcg aacaattcgg ctaccatcag
     5821 cacgtaatca gctttatagt ctgcccgtaa acttttcatc gccgcagccg atatttttgc
     5881 cgggtaatac aaccaaccat acatcagccc ggcggttgcg ccaagcataa tggcgagaat
     5941 gaaggcgatc aacctgcctt tgcgcatcta ctgatccttc ggcagcatgg gcatgtgaat
     6001 gcccaaccgc ctggcggcca taatggcctc ggggtatccg gcgtcagcgt gcctgaccac
     6061 ccccatgccc gggtcggtag tcaacacgcg ctccaggcgt ttggcagcct gcggggtgcc
     6121 atccgccaca atgaccatgc cagcgtgctg gctgaaaccg attcctaccc cgccgccatg
     6181 atggaatgaa acccaggtcg ccccgcccgc ggcgttgacc agcgcgttga ggatcggcca
     6241 gtcactgatg gcatccgagc cgtcttgcat cccctctgtt tcgcggttag gggaggcgac
     6301 cgagccagca tccaaatggt cgcgcccaat gacaatgggc gcttttaatt gacctctggc
     6361 aaccatttca ttaaataaca gtcctgctcg cgctcgctca ccatacccca gccagcagat
     6421 gcgcgagggc agcccctgga aatgcacccg ctctcgcgcc atggttaacc agcgcttgag
     6481 atgctcgtca cgcggaaaca acttactgat ggcttcgtcc gtgcggtaaa tatcttcggg
     6541 gtcacctgaa agcgccaccc acctgaaggg accttttccc tcgcaaaaca acgggcggat
     6601 atacgccggc acaaaaccgg ggaattgaaa agcgtctttt acaccgttat caaaggcgcg
     6661 ctggcgcagg ttgttgccat aatcgaacac ctcagcgccc ttgcgttgga aatgcagcat
     6721 ggcgcggatg tgcgccgaca tcgaatccag tgagcgatgc tcgtattgcc gcgggtcatc
     6781 actgcgcatc ctctctgcct cttctagtga aagccccgcc gggatgtaac tcattacatc
     6841 atgcgccggc gtctggtcgg tcaccacatc tggcaccacg ccgcgcagca ccagttcggg
     6901 gtaaatttca gcggcattac cgatgagcgc gatggaacgg ggttcttctc gctggcaaaa
     6961 ttcctccacc agggtcatgg cctcttccag cgaatccacc accacatcca cataacctgt
     7021 ttccaggcgg cgttgcgctc gctgggggtc aacctcaacg cacagtccca caccttcgtt
     7081 catggtaata gccagtggct gcgcgccgcc catgcctccc agaccggcgg tcagcacaaa
     7141 ccgaccgcgt aatgattgcc agcccttttg cctggccagc gatcccaggg tttcgtaagt
     7201 cccctgcaaa atcccctggg tgccaatata aatccacgag cccgctgtca tctgcccgaa
     7261 catgatgagc ccttttgccg ccagttggtc aaaatgctgc tgcgtggccc aatgcggaac
     7321 caggttggaa ttggcgataa gcactcgcgg cgcgtcagcg tggcttttaa aaacaaccac
     7381 cggcttgccc gattgcacca gcagtgtttc atccggttcg agttcacgca aagatcgcag
     7441 gatggcatca aaggattccc agttgcgcgc cgcctgcccg cgcccaccat acaccaccag
     7501 gtcttgcggg cgttccgcca cctcggggtc aaggttattt tggatcatgc ggtatgcggc
     7561 ttcgatctgc cagttcttac aggtgagcgt actcccgcgg ggagccctga tcacgcgtgt
     7621 ttccaccatg cgtcctccgt tcttttccgg cctgtttttt cgattattat acgctgaaat
     7681 acaaacccct tacgccgcac gcgttacggg ctgctatcag ggatgataac cgcacctcaa
     7741 atctgcctgg cgtaagcgag cagcaccggc ggtgatattt gcccgcgcaa cccgccctgg
     7801 cgcagaacca gccgcgtaag aagcgggcgc ttttttaagc gatacgccag gaattccgcc
     7861 acgcggccag gttcttccag cgctttaaaa gtggcacggg tgcaatgctc acccacctgt
     7921 atctccaccc ttggctggga ttgcgcgttg cggtaccaat cagtccacgg ccatccgcag
     7981 cgccttccca gtacgccccg tcgatccatt catactgcag tccaactgtg tgaggtttgc
     8041 cgctccgcct gccagtggtg gtaagcagca agaccatctc gccgacgagt cgatacaaaa
     8101 aaatgcgata aagccgggtg aatttatggt tgtctggcct gaaccttatg taacgggccg
     8161 ttcactcgta cttgagcgcg ttaaccggca ccaaagatgc agcgcgcagc gccgggtaca
     8221 accctgaaag cagcccgatg acggtagcaa aaaccagcgc aaaaacaggc agccagaagg
     8281 gcgttgatgt ggcggctgta ggcagagagc cgcccccgtt ggcagcctgg ctggcaaaat
     8341 acgaaagcgc caccacattg aggatagcgc tggctcccca gcccagggcc acgccaccca
     8401 ggccgcccac aaaaccaatg ccggcggctt cgcccaggaa aatgctcagc acgtcgcgat
     8461 tggtggctcc caccgctttc atcagtccaa tctcgcgcgt gcgctcgagg atggccatgg
     8521 ccatggtgtt ggcaatacca atcgccgcca ccagcagcgc gatggcgccg acgcctccga
     8581 agatgatttg cagcaccagg aagaaagaat tgataccctc caggtaagat tgcggcgtat
     8641 tcgccatgta acccagggca ttgatctggt cagcgatatc caatactaca tcggggtctt
     8701 ccgccttgac gatgagcatg ccatagcctt ctttatcgcg gttgatgcgt gtccctcgtc
     8761 cccattcgct aaaagccgtc acttcatcca ggcgcatgta catattcccg tccgccatgc
     8821 cgcgcgtttc ttcaaggata cccacaacgc gcacattgaa cgtacgtcga acttcttccc
     8881 catcctgtgt gtatttcacc agcaccaatc gaatcacctg gtcgagcagt tctggggtcc
     8941 cggtcatctc ttcgcccggc cgcgggttag gattgtaaaa attctgcggt acccaggacc
     9001 caatgaccat ggtgcccctg gacaattcag tggtgccgcg cgccgctttc aggcccatat
     9061 ccgccaggtc atcgatgttg acgcctatga tacctcccca ggacatcagc ttgccaaaat
     9121 ggacctccga gcctgattgc aaataatcat ggatgatggt cttgcgcaca ccagggatgg
     9181 cggatatctc cttaatagcc gcgggtgtga gcagtttcat gtctgatccg ccgcctgcat
     9241 acataacgcc gccacctcca ccccctccca tcacccttga ttcctggtat cccgggtata
     9301 cttcaatgct ggttaggtcg ctgatgcccc ataattgctg ggtggcgctc ttctgcaacc
     9361 ccacagccag cgaaaccagc accaccaccg cggcggtgcc aatcaccacc cccaccgcgg
     9421 tgagcagcac ccgccccttg cgcctgccaa ggttatagag aatcaggctg acgagatcga
     9481 taaaacgcat cgcccgacct taccctttag ggattgcggg taccgggata acttctccac
     9541 tgggtgtttc ttgcactgga taggtctcta ccggctgctg gagtccacta tccagcccaa
     9601 agagcccttt aaagaacctc accacctttt gccagaaagt ctcaggcgcg ggtactacag
     9661 gcccctctcc gttcggcgtg ttgccatccc ccatatctgg cggtacttcc atggcaggct
     9721 ggacctcaac gggaataacc tgttcaatga aacgcggttg gttgaaatca tccgtgtaat
     9781 tgatgacaac cttcaggtcc agcggcccct cctggtaagg cgtcaggctg gcatccaggg
     9841 tgtaataacc gcccgggtcc agcgcgccga ccagcaggat attattaagc aggtcggcgt
     9901 tctccgcgct caccttcata ttaccgagca cgtaggtttt cttgcccaga ttggtcacct
     9961 gcagcggcag aatgttttcc atgccagcgg taattatgcc cgcgtcacgg taaaaactca
    10021 cctctacctg gggtaatgaa tacaccagca gggtgatgac ctggtcatcc accaatcggt
    10081 tacctttggc atcgatatag acaaaagaca acttcagcgg gtaagctccg ggctgagcag
    10141 taacattcac caccagcttc tgggtcacca ccgcagtctg gtctttgctg acgtcgccca
    10201 taaagataac gttggaactg cccagcggcg cgaaagtggt caggtcactg ctacctcccg
    10261 atacgcctcc cggttgcggt ttgccctcct cattgctggg catacctccc ccgccgatga
    10321 cagcggagac gtttttcgcg tccgccatgc ccaggttctt gacatccagt ttcaattcaa
    10381 aaatcgagcc gggctgcaga ggatcaatgt ccgtgctgta gccagtcacc accagttgtg
    10441 gtcgttgcgg gatgttgggg gtggccgtag cggcaaaccc agaagaagag ggctcagcga
    10501 tgacaaaaga gagggtgaag acttcgctgt aagcggtgcc aagcgggtca gtgtagttca
    10561 ccgtggcttt catactggcc acgttggccc aggccagggc atcgccaatg agaaattttt
    10621 gggtgatggt attggtgttg cccgggggga gcgtgccggt ggtgcgcacc ccgccgctct
    10681 cacgcggata aaagtcagtc ccgtcgaaag tgacgatgat attttcagcg cttaccttgc
    10741 cgttattccc cagcaccacc tggaggttca tttcattccc agcgaagagc gttttattgc
    10801 ccgtcgaata agaggtgatg gtgaccatcg gtctcccgat agcgggtttg ggtaccacca
    10861 ctgtggtcgt aagctgcagg gggtcgaatt ctacaggatt gcccgccact actgttacgt
    10921 ccaccgttgt aatgtcgctc tgaccctcag tagcgccaac agggatggga acgttcacgc
    10981 tgaccgtctt cgtctcattg gcaccaaagg taattgtgtt tggaaccacc gtgggtgagt
    11041 tccaaccaga agtgctgttc gcagacacgt tcattatttg cccagcctca ccaggaatgt
    11101 ccaacttgta agtaataaca gaacccggca agccgctctt ggatttgcta tcaactggca
    11161 cttcatcgtt caacggtgcc gcgtatgtgt tgagtgtggt cattccgaat agaaaggtca
    11221 aggtgagtaa aaggcctaaa acattgcggt tttttttgat cataggatct cttcctgttc
    11281 tacaatgggt tgggtcatat tttcggtggc tttgcgataa gcctcttcag aaaccacctt
    11341 gccatccagc aggtaaagca tgttggtagc aaactggcgc atgcgcgggt cgtgcgtcac
    11401 cacgatcacc gttttgcctg cctggtgcag gcgtgaaagc agcttcatga tggtgaaccc
    11461 gctctggctg tcgaggttgc cggtcggttc atccgccagt atcagcggcg ggtcgttgat
    11521 taacgcgcgg gcaacggcta cgcgttgctg ctgcccgcct gaaagctcgg tgggtttatg
    11581 ggtggcgcgg tcgctcaagc caaccttttg taaaatttcc tgggcgcgcg cgtgcctttg
    11641 cgccctgggg atgcgagcaa agcgcagcgg gaagctcaca ttctcttccg cgggcatgga
    11701 aggaatcagg ttgaaggatt ggaaaatgaa ccccacacgg ttgcgcctgt actcagccag
    11761 caagttttcg tccatctttt cgatctcgtc ctcgccgatt gtgatactgc ctgaagtcgg
    11821 ccaatccaac ccgcctaaga ggtagagcaa ggtgctcttg cctgaaccgg aaggtcccat
    11881 caccaccgtg aacgaccctg ggtcaatttc caggctgacc ccatccagcg cgaccacggt
    11941 gttttcaccc atggtatagc gtttggtcag gtctcgtgtg cgtaccagag ccttatcgag
    12001 catccttatc ctcactcctg ttagaagtag aaaccgcctc tcaatgagag ccctcccagg
    12061 gctgaagcga gcaacccggg aatggccatc agcagcagca cgatcaccac agaaattgcc
    12121 gtggcagtcc aggctttgga acgggataaa ccggaaagcg gaacggagcc gagcagcagc
    12181 aaggcaattt gaaagaggaa gtagatgtcg ataaaaccga gcatgctgcg caagtaagcc
    12241 ccaaccccgg tggcttcagc agcgataaaa ccagaaagac ctggggcgct caccactgtt
    12301 ttgctcacta tcatcgccaa aatctgtatc agcaaacgga cggccatggg cagcatggac
    12361 catgccacca ggttatacga tttgatactg ccagcgcggc tgcccgccag cgtaaggctg
    12421 aggtacatca ggatgctgag cagaaaccag ggcatccaga ccttcagcag cccggaaatt
    12481 aacgggaaca cgtacaggaa caacggagaa gtctgctttg cttgggcggc aaaaaactgt
    12541 tcctgcatct cgggggtgta gtattggaag tcaggcggaa gttcactgcc catctggatg
    12601 acattccgct tgatgggagc accgatggcc accgccagaa tcaccagcac agagagaata
    12661 agcagagggg tcagccatgc cgctttttcc tgcgtcacga tttcagaagt ggtgcgcgcc
    12721 gggcgcgtga ataaggaggg aatccatttt gtgttccagg agtgtttatt ggtttgttga
    12781 cctttcgcat ttaatgccaa agcgcacctc tttttagggt tctttcagca taagacgcat
    12841 ttatcatact acaagttccc gcggcagccg ttatcaaaaa aatcgttatg ctataatcgc
    12901 caaaggaggg caccatgcgc atcaatattg gtgtggagaa caattacgaa ggcagatcgc
    12961 tcgcctggat gttggattac ccgggttgtt ttgcctacgg cgctgagggt agcgaggcca
    13021 tcctccgcgc gccgcaggcg ctgctgcgtt ataaagaatg ggttgacagt cacctgaaag
    13081 actcctggat gaaaagcctg ggtgatttcg acatccacct ggaagaaagt tatgacgttt
    13141 tcagcatcaa tgatgatttc gacctcgcag acgaagggat tgaggtgaac gcctggttcc
    13201 gtcacgactg gaaacctctc actgcagaag atatccgccg ggggacgctg gtactaacct
    13261 ggagccggga agacctgctc gagctggttt cggggttaac tcccgcccaa ttggactgcc
    13321 gtttttcagg cgagcgttgg agcattgccg gtgtgctgga gcatatcgcc aacgctgaac
    13381 actggtacct tcaccgcctt ggcctggcgg ttggaacccg ccaggaactg cccgcggatg
    13441 tttttgagcg cctgaaggtg gtacgccgcc gcctggtaga ggtgctgcca tccttgcaag
    13501 gcagccgcca ggtgcttggg gtggatggag aattctggtc gcctcgtaaa gtactgcgtc
    13561 ggtctgcctg gcacgaagtc gatcacattg aacatatctt caagttaatc tccaggctat
    13621 aaaaaacgcc tccgcgcgga ggcatttttt tgcgaacatc aggagggagg ttgcgggtcg
    13681 ttttacttga gcgaagtatc aagcccaagc aggctgaacg ccagaaacat caaaccaaat
    13741 acggctatca ccagatacac aatcatcgcc atcgcacacc tccaatttaa cctttctctc
    13801 gcgctccttt taaccagtca atttctcatt aattgatctt attcgccttc ttaaatacgt
    13861 ataaccccga taaaagttcc cgggtgctgg ttggatcttg gaaatagacg cgcttaatga
    13921 atttgtaaac tcaaacggga aaccgccatc atccgttggc gtggcctgct tgcggtctgc
    13981 ccgaccaaac cccggcaatg ggctgaccgc aaaaggcgca ggcgccggcg ctgttgatat
    14041 gatacccatg cagcaggtaa ccgcgccttt cgatcaccgc cctgccgcac tgcgggcaaa
    14101 tggtgttttc cagcgagccc acgcgccccg gaaggtttcc agcgtaaaca aaacgcagcc
    14161 cggcttcctg accgatctcg gcggcgcgct gcagcgtttc cgccggggtg gggggcgcgt
    14221 tttctttgta atccgggtga tatgccgtca catgccaggg aatatccggc gaaatggaag
    14281 ccaggaaacg acctgcctcc caaagctcat ctgaagaatc gttcatcccg ggtattacca
    14341 gcgttaccac ctccaaccac agccccaact ctttgatgcg ccggatggta tccagcactg
    14401 gctgcagtcg gccgcccatc ttcctgtact cgctgtcgcg catgcttttc aggtctactt
    14461 tatagccgtc cagccagggc accaggtagt ccagcacttc tgcggtggcg taaccgttag
    14521 aaacatacgc ggttttcagc cctttgtctt tggcgagttt aaacacctca actgcccatt
    14581 cagtggtaat gagcggctca ttataggagg aggcgatggc ccgcgcgcca ttttctacag
    14641 cgtaggtcac cagcttttcg gcggaaaccc gcctgatagc ataggccgac ctgccggctt
    14701 cagggtcacg cagcgcctgc gaagagagcc agttctgaca gaaaccacaa tgaaagttgc
    14761 agcccaacat accaaaggtc aacacttcgc tgccgggtaa aaaatggttg aagggctttt
    14821 tttctagcgg gtcgatctgc acgccggcca catatccttt cggggccatt aaaacgccgt
    14881 tggcgttgaa ccgcacgccg caaataccgc ggcgcccggg tttcagcaag cagcggtgcg
    14941 cgcaagccag acagcgcagg ttgccctgtt ccagtgtttc caccagttct gcgggctggg
    15001 tgagcacatc gagtaattca tgcgggttca tctgtcgctc catatccatt atagcccgcg
    15061 gcggcatatt tcaatccgct gtactcgttc aatacatatg agatttttcc gaggttacct
    15121 ctgggtgata ttt
//
LOCUS       DAOSPM010000045        14110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_4337_length_14110_cov_10.166133, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000045 DAOSPM010000000
VERSION     DAOSPM010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4337_length_14110_cov_10.166133"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1951
                     /locus_tag="PLW25_05450"
     CDS             <1..>1951
                     /locus_tag="PLW25_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOS53969.1"
                     /translation="GSYKAVQGIETVDTASNLRGVGNGADHIIITPRAFWEQAETLRA
                     HRAGQGLRAVKVDLQDVYDEFNYGIASAEAIRDFLEYTYNAWVKPSPSYVVLMGDGHF
                     DAKNYQNLGKVNYIPPYLAFVDPWLGETAADNRYVTFKDRMGNRDAMPDMMLGRMAVN
                     SAAEAAAMVNKTIAYEANPAEPWQQQVLAVADNRDSGGLFDLLSDSLLAGHLPAPYTA
                     EKVYLNVTHMTAADAKTAILAGINSGKLLVNYIGHASRTDWATEKLFGVADVKNLANG
                     SKQPIILGMTCYDGYYQMPYLSDYFATAEVVTRGAKFDGAEDKGAVASWSPSGLGVAS
                     GHDRLNRGFFDALLTDGVETLGQATYAGKAELWASGTNYDLLETFLLFGDPALRVNRV
                     PALKVSGNITTPSGGPFSGVEVSTTLATGQIRKATSNVDGKFLFNGLPRGEYVFTPLM
                     TGYNFNPAASAPQVLDDSDLSGVNFTSSLAPIFSISGTVKDYQGQVLDDVTVSIDNGQ
                     APVVTQSDGKYAFGELTLGTYQVSVSRAGYLFPVPSQSVTISGVSRTNINFSGLPLHS
                     ISGRITYNGAPLPGVTVDDALGHTALSGVDGTYLFEDLTPEVYTLTPSLEGYAFVPGS
                     RQVTLSAVDVPGMNFSADKLGVNDPP"
     assembly_gap    1952..2051
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2052..>3256
                     /locus_tag="PLW25_05455"
     CDS             <2052..>3256
                     /locus_tag="PLW25_05455"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOS53970.1"
                     /translation="GLYGEDAVTFTVTPVNDAPVMVENIPDQWVIHGAAFAPIELDRY
                     ISDVDNSADEINWSVAGDTNLDVTIIDRVATITQREANWSGSETVTFRGTDPEGLFAE
                     QPVNFDVVAVKPTVGQKLTAKRVTFEWSGITNANQYNVQLSLSDTFDKILLNATTSNL
                     RFAYGSDLANGKTYYWRIRPRFGTTWGDWSTVKTSDQKPLEFLSMNPPLAPTLKAPAA
                     GAFLRVKDVDFDWTDVVNADHYQLQISKTTAFKDPNTYYPQPGDQPFTVHDLQDGLYY
                     WRIRAIDEVDVAGGWSATRSFTVDTIAPAAPALIAPLAGAYTYDTTPTLAVKAVPGAT
                     WYRYRVVTKAEGEPATCEQNVVLTSDKLNKTSWSTMTPELPYGDTYYWCAQAVDAAEN
                     ESPWSESEAR"
     assembly_gap    3257..3356
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3357..5669
                     /locus_tag="PLW25_05460"
     CDS             <3357..5669
                     /locus_tag="PLW25_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53971.1"
                     /translation="AAYRIVVDNHSNFSSPEYTAVVGTTTHTLGTDVISMQPPGFNKL
                     NDGRYYWKVQALDFRGNAISNAWSAVWSFIVDTIQPAPGLNLPQNGTTVQGTPTFSWK
                     AVPGAKYYELWVDEADGDFSDPVYKKSMLTTLNHKPPIIMEGGYRWRVIASDLAGNES
                     EGGEGWIVTVNPLKPVAPVLTGPANGVFLNDEKTEIVLSWNPVAYAQRYEFRVDDDPR
                     FTSPAYSESSGNETSYLLTDTAAHLLDNTRYYWQVRAVNSAGAETGAGAWSAARSFTV
                     DRTPPVNPLLTLPANSGTVQGTPTFSWKAVPGAKYYELWVDEADGDFSDPVYKKSMLT
                     TLNHKPPIIMEGGYRWRVIASDLAGNESEGGEGWIVTVNPLKPVAPVLTGPANGVFLN
                     DEKTEIVLSWNPVAYAQRYEFRVDDDPRFTSPAYSESSGNETSYLLTDTAAHLLDNTR
                     YYWQVRAVNSAGAETGAGAWSAVRSFTVDRTPPVNAPGLRTPANEASMATYTPALSVN
                     SLKDAKWYRFTVYQSGTCEANDGFISPDVTATSWAVPAAQALAYGKYHWCVQAVDAAG
                     NASSWSEARVFTLTFQNQPGFAATTASATPGFSWYAVRGARGYRLEVKPVVEEGNTAT
                     REYSVYLGTVTSHVIPAIKALDPGAYQWRLRVYTAAGSEETPWRLLVVLPPAPVLDTP
                     DNGSVHPGVTVELNWFPAAGAARYEIQADNNNNFRTPEIDYEFTEGPVTTKEVTFPFA
                     GGFYWRVRAYNYLGNPGAWSAARYLRLTTP"
     gene            complement(5813..7666)
                     /locus_tag="PLW25_05465"
     CDS             complement(5813..7666)
                     /locus_tag="PLW25_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53972.1"
                     /translation="MRVRGAFSYLTGKGILLVCLTVLTVALLITNSVNIHPASAQDGP
                     DRVTWITVDDSQYDWWLARWSDNTIVCQFTTAHAGTPSELEIQKFCGDQVLNTWLTTP
                     PCTAGEEGKDLSSCSGLYLQNTRSSLSANEIMVNLPPPVVWITVNGSVLPGTTNKWEG
                     RPEVVLIGEEKLANESITRIHGVYGGTKFSCAGSRCPLPLWQTGEQGVMLTFWGESSY
                     GDTTEEYTAYIRVLPWADMPADNAGAPERSGYYVDIISSQWQAQQMSSCAAIWQSFPS
                     VEGLPAWLKNPADRASLSTSLPLHYLAAMLIQNGVVDAGDCPYGGLDYYASANQCGME
                     AAMQAVTLWQNQFDDEIIRTSIETGIPSILLKNIFAHESQLWPGIYHNAEEVGFGQLT
                     EDGAEAALLWNPEFYTQFCPLVLYEDTCARGYGNLSQEQQTTLRGALVQKTNATCPDC
                     DLGIDLTQANFSVKIFGETIVGNCAQVDRMLYNLTGASAGAVASHDDLWRYTLVNYNA
                     GPGCLWHALKRTWEAGNRLDWLHVAANLEGGCRAAVNYVMDISGGDTHPVMVYSTSLP
                     SPTITPTIVRATATLTKTPSFTTTPGTATPTGSITPTPSPTKTSAWGTVGP"
     gene            7805..8290
                     /locus_tag="PLW25_05470"
     CDS             7805..8290
                     /locus_tag="PLW25_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOS53973.1"
                     /translation="MQERAQGGLLEPQTYSISGSDLVALMEAVLAKNGTFRFTASGSS
                     MAPFIQTGDMVTISSLHKGRVGLGTVVAFKHPVGGHLVIHRVVGVAHGTFLIKGDNTP
                     HRSDGWIPSGCLIGRVLEITRNGRRVWLGLGVERYLIAFFSRWQWLIPLRGALAALRR
                     R"
     gene            8434..10146
                     /locus_tag="PLW25_05475"
     CDS             8434..10146
                     /locus_tag="PLW25_05475"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOS53974.1"
                     /translation="MDNELEERSSLESILDYAQTLWRWAWLLLIVAIAAGAVAFYLTN
                     QKPRVYESSTRAVVNVVTGSNFYDAYSASFGAQRLAETYSQTMITPELLQSVSERLGF
                     EVTGKITVTPVENSPIFTIVVTDNDPQKAADTANMLITIFAEKVMKDQSSRYSELKQG
                     LEEEIARIDQNLTDINERLAILQIKEAELAEAEKRAAQMAAEGLEPIEVVRDPNDYVT
                     RAQLELAQSQYQSTRSSLFYNLQQIKLTEVQSATTISQIDKAMANPEPIQPQPFKSAG
                     LAAIVGLMVAAGIVFLIAFLQDEIRDPEEFARRWGVPVVGVITHYPSNQDKLITVAKP
                     RMSVSEAFRSLRTNLQFSGIDTPLKSVLVTSPSPSEGKSSVVANLATVIAQSEKEVVL
                     VDGDLRRPTLHKVFKLSNRIGLSDYFIREPEKMSGVVKKTKVNGLNIISSGSLPPNPS
                     ELLGSTKMHDVLGLLEKHFDMVLLDSPPLLAVTDALILAKSVDGVLLVVDPKKTKRGA
                     IRQAIEQLQRLDARLLGVVLNNIKIKRSSYYYNRDYYYSKQYGKSNEDGTHEEPAVEE
                     DKTR"
     gene            10162..11064
                     /locus_tag="PLW25_05480"
     CDS             10162..11064
                     /locus_tag="PLW25_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53975.1"
                     /translation="MTGEPNPAVKGTKTDKKRNDTLFCPFLGFEDDPTTALAYPAKLN
                     FCFRAKPAAPVSLAHQRDKCLTEFYVDCPVFQSENPIPLPESIRGERKSKTFQFNWVP
                     LVALIAVVIIGLVALLLTGVFTFRGFTPPLADISTATHTPAHLPVVPLSAEPTTTVEA
                     TATLVPTVTATWLIPGKHTPRALETPFGTSPQLVIHKVAAGEGFILLAEKFHTTADAI
                     KAINYQLPDSLWENTLLVIPVNTDDVAGLPAFSVVEIRAEGMTIESYAARMQLDVEML
                     KKYNDLPEGYSFQIGEMLIVPMTE"
     gene            11088..11591
                     /locus_tag="PLW25_05485"
     CDS             11088..11591
                     /locus_tag="PLW25_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS53976.1"
                     /translation="MPNVLFVCSANRFRSVLAAEYFTSLLEKQAPSGEWVVGSAGIWA
                     REGVAPTKEALQWAAGKQLHIESVRSREISNALIVEADLIIVMTNGQREAISIEFPEA
                     REKLTLLSEIAAAQIYDIPDPIIESGEEPLQIAEEISTLIDAGFERILKRAAGRANNL
                     GAQLRGA"
     gene            11581..12603
                     /locus_tag="PLW25_05490"
     CDS             11581..12603
                     /locus_tag="PLW25_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOS53977.1"
                     /translation="MEHKPPNAKRRLLWLLLVLLFLVSCGYLLWTNPLLGKRLPQFTY
                     EPLATFTAIPTSAALTLTPETVKTEPALATVEPEPTSVSPVCGQTQPMIILALGIDES
                     EQADMIRVVRVDWLKHQVVVLSIPRDFWVPIPGLEEHNISQFRINAAYGYGEYFNGRG
                     QGVVKFSETMYQNYGVTFDRYVVAHFNTFTELVDQIGGVDIDLEEPIGAYASAGHHHF
                     DGKSALLFVRQRIADLDAYRIQRQSAILKGLYEKLSKPEYLVQLPGLGVKFIKDKSVI
                     TDLRLQDVYTFNCFIREINRDSLVFIDIPYDLYTPMITNYGRHIKLPKPEAGVFIQNV
                     IKNGTE"
     gene            12936..>14110
                     /locus_tag="PLW25_05495"
     CDS             12936..>14110
                     /locus_tag="PLW25_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOS53978.1"
                     /translation="MSELINNKQKRVELMKSLIRQLHQGMAEDRVRMQLETLLDEADY
                     NDVFVMEVQLIQEGIPAEVVQDLCDTHTRVLKKHLDLQETPQTIPGHPVHTFVEENRE
                     LTKVTAQIRHLMSLIAEKPVEEDVSEQMGEIHQLLNNLMDVEKHYQRKENLLFPYFEK
                     NNLPGPPAVMWGKHDEVRELLKTTLEGFQQVKSFSAGEAAAFNQFSVSMVVDAIDEMI
                     YKEEKILLPTALDILSEEDWYAIYLESDEGGYCLYVPQFTWTPEGDFKREIRNPSGVG
                     ARIQMPTGSFTLDELVALFSTLPFDLTFVDKDDSVRYFSPGTERIFKRTRAILGRKVQ
                     YCHPPKSVHIVNRIVKDFKEGKQDQARFWINKGGRMVYIVYYAVRDEQGGYLGTLEVT
                     "
BASE COUNT         3291 a         3625 c         3976 g         3018 t
ORIGIN      
        1 cggcagttac aaggcggtgc aggggataga gacggtggac acggcctcga acctgagggg
       61 ggtagggaac ggggcggatc acatcatcat cacgccgcgg gcgttctggg agcaggcgga
      121 gacgctgcgg gcgcaccgcg ctgggcaggg gctgcgggcg gtgaaggtgg acctgcagga
      181 cgtgtacgat gaattcaatt atgggattgc ttcggcggag gcgattcgcg actttttgga
      241 atacacctac aacgcgtggg tgaagccgtc gccgtcgtac gtggtgttga tgggggatgg
      301 gcattttgac gcgaagaatt atcaaaacct tgggaaggtg aactacattc cgccctacct
      361 ggcgtttgtg gatccgtggc tgggagagac agcggcggat aaccgatatg tgaccttcaa
      421 ggataggatg ggcaaccgcg acgccatgcc ggatatgatg ctgggcagga tggcggtgaa
      481 cagcgcggcg gaagcggcgg cgatggtgaa caagaccatc gcctacgaag ccaaccctgc
      541 ggagccctgg cagcagcagg tgctggcggt ggccgataac cgtgacagcg gcggtctctt
      601 tgacctgctt tctgacagcc tgctcgccgg gcacctgccc gcgccgtaca ccgcagagaa
      661 ggtgtacctc aatgtgaccc acatgacggc agccgacgct aaaacggcga tcctcgccgg
      721 catcaactcg ggcaaactgc tggtcaatta tattggtcac gcttctagaa ccgattgggc
      781 gaccgaaaaa ctattcggcg tggcggatgt caaaaacctg gcaaatggca gcaagcagcc
      841 catcatcctg gggatgacct gctacgatgg ttattatcaa atgccatacc taagcgatta
      901 cttcgccact gccgaggtgg tcacccgcgg cgcgaagttc gatggcgcgg aggacaaagg
      961 cgcggtggcc agctggtcgc ccagcgggtt gggcgtggcc agcgggcatg accggcttaa
     1021 ccgcggcttc tttgacgccc tgttgaccga cggtgtggaa accctgggcc aggcgactta
     1081 cgccggcaag gctgaacttt gggcttcagg gacgaattac gacctgttgg agaccttctt
     1141 gctgtttggc gaccctgcgc tgcgcgtcaa ccgcgttccc gcgctcaaag tctcaggtaa
     1201 catcaccacc ccctcaggcg gaccgttctc cggggtggag gtatccacaa cgctggcaac
     1261 cggccagatc cgcaaggcga cgagcaacgt tgacgggaaa ttcctgttca acgggctgcc
     1321 gcgcggtgag tacgtgttca ctcctcttat gaccggctac aactttaacc ctgccgcttc
     1381 cgctccgcag gtgttggacg acagcgacct gagcggcgtg aatttcacct ccagcctggc
     1441 gccgattttt tccatctcag gcacggtgaa ggactaccag gggcaggtgc tcgatgacgt
     1501 taccgtctcc attgacaacg ggcaggcacc cgtggtcacg caaagcgatg gtaagtacgc
     1561 ttttggcgaa ctgaccctgg gcacctacca ggtgagcgtt tcgcgggcgg gttatctctt
     1621 ccccgtgccc agccaatcgg tcaccatcag cggcgtgagc cgaaccaata tcaacttttc
     1681 cggtctgccg ctgcattcga tctccggacg tattacctac aacggcgcgc ctttgccagg
     1741 tgtgaccgtg gatgacgcgc tcgggcacac ggcgctgagc ggcgtggatg gcacatacct
     1801 gttcgaggac ctcacccctg aggtctacac cctcaccccc tcgctggaag gatacgcttt
     1861 cgtaccgggc tcacggcagg tgaccctttc cgcggtggat gtgcccggga tgaatttttc
     1921 cgccgacaag ctgggggtga atgacccgcc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn ngggctgtat ggcgaagacg cggtgacctt taccgtgacc ccggtcaatg
     2101 acgcgccggt gatggttgag aacatcccgg accaatgggt gatacacggc gctgcttttg
     2161 cgcccatcga actggatcgt tatatctcgg atgtggataa tagcgcggat gagataaact
     2221 ggtctgttgc aggcgacacc aacctcgacg tcacgatcat cgaccgcgtg gccaccatca
     2281 cccagcgcga ggcgaactgg tcaggcagtg agacggtcac tttcaggggc acagaccctg
     2341 aagggctgtt tgccgagcaa ccggtgaact ttgacgtcgt cgcggttaaa cctaccgtgg
     2401 ggcagaagct gactgctaaa agagtgacct tcgaatggtc gggtatcacg aacgccaacc
     2461 agtacaacgt ccagttatct ctgtcagaca cttttgacaa aattctgctg aacgctacca
     2521 catcgaacct gcgctttgcc tacggcagcg accttgccaa tggaaaaacc tattactggc
     2581 gaatccgccc gcgcttcggc accacctggg gagattggtc tacggtgaaa accagcgacc
     2641 agaaaccgtt ggaattcctt tccatgaacc cgccgctggc gcccacgctg aaagcgccgg
     2701 ctgctggggc tttcttgcgg gtgaaagacg tggactttga ctggacggat gtggtcaatg
     2761 ccgaccacta ccagctgcag atcagtaaaa caaccgcttt taaggacccg aacacctatt
     2821 acccgcaacc aggtgaccag ccgtttacgg tgcatgacct gcaagacggg ctatattatt
     2881 ggcgcatcag agcgattgat gaagtggacg tggcgggcgg ctggtctgcg acgcgtagtt
     2941 tcactgtaga cacgatcgcg cctgccgcgc cagcgctgat tgccccgtta gccggcgcct
     3001 acacttacga taccactccc acgctggcgg tgaaagccgt gcctggcgcc acctggtacc
     3061 gctaccgcgt ggtgaccaaa gcagaaggcg aaccagcgac ctgtgaacag aacgtcgtgc
     3121 ttaccagcga taaactcaac aagaccagct ggagcacgat gaccccggaa ctgccctatg
     3181 gcgacaccta ctactggtgc gcgcaggcag tcgatgcggc ggagaacgaa tcgccctggt
     3241 cagaatcaga ggcgcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgg
     3361 cttaccggat tgtggtggat aaccacagca atttctcctc ccctgaatat accgcggtgg
     3421 taggcactac cactcacacg ctggggacgg atgtgatttc gatgcagcct ccaggcttca
     3481 acaagctgaa cgatgggagg tattactgga aggtccaggc gctggatttc cggggtaacg
     3541 cgatctccaa tgcctggagc gctgtctggt cattcatcgt ggacaccatc caaccagcgc
     3601 cggggttgaa cttaccgcaa aatggcacaa ccgttcaggg cacgccgacc ttcagttgga
     3661 aagcggtgcc tggggcgaag tactacgaat tatgggttga tgaagcggat ggcgatttct
     3721 cagacccggt ctataaaaaa tcaatgctga cgacgttgaa ccacaaacct ccgatcatca
     3781 tggaaggggg atatcgctgg cgggtgattg ccagtgacct ggcgggcaat gaatcagagg
     3841 ggggcgaggg ctggattgtt acggtcaacc cgttgaagcc tgtggcgccg gtgctgaccg
     3901 ggcctgccaa cggcgtattt ctcaatgatg agaaaacgga gatcgtgctg agctggaacc
     3961 cggtggctta cgcgcagcgc tacgaattca gggtggacga cgacccgcgc tttacctccc
     4021 ctgcatattc ggaatcgtcg ggaaatgaaa cttcgtatct gctgactgac actgccgcgc
     4081 atttgttgga taataccagg tactactggc aggtgcgggc ggtgaactcg gctggggcgg
     4141 agacaggggc tggagcgtgg agcgcggctc gttccttcac ggtggaccgg acgccgccgg
     4201 taaatccttt gcttacccta cccgcaaaca gtggaaccgt tcagggcacg ccgaccttca
     4261 gttggaaagc ggtgcctggg gcgaagtact acgaattatg ggttgatgaa gcggatggcg
     4321 atttctcaga cccggtctat aaaaaatcaa tgctgacgac gttgaaccac aaacctccga
     4381 tcatcatgga agggggatat cgctggcggg tgattgccag tgacctggcg ggcaatgaat
     4441 cagagggggg cgagggctgg attgttacgg tcaacccgtt gaagcctgtg gcgccggtgc
     4501 tgaccgggcc tgccaacggc gtatttctca atgatgagaa aacggagatc gtgctgagct
     4561 ggaacccggt ggcttacgcg cagcgctacg aattcagggt ggacgacgac ccgcgcttta
     4621 cctcccctgc atattcggaa tcgtcgggaa atgaaacttc gtatctgctg actgacactg
     4681 ccgcgcattt gttggataat accaggtact actggcaggt gcgggcggtg aactcggctg
     4741 gggcggagac aggggctgga gcgtggagcg cggttcgctc cttcacggtg gaccgtacgc
     4801 cgccggttaa tgccccgggg ttgagaacac ccgcgaatga agcatctatg gcgacctata
     4861 cccccgcgct ttcggtaaat tcgctgaaag acgcgaaatg gtaccgcttt accgtgtacc
     4921 agtcaggcac ctgcgaggcg aacgacggat tcatcagccc ggatgtgacc gccaccagct
     4981 gggcagtacc ggctgcccag gcgctggctt atggtaagta tcactggtgc gtgcaggcgg
     5041 tcgacgcggc gggcaacgca tcctcgtggt cggaggcgcg cgtgttcacc ctcacgttcc
     5101 agaaccagcc cgggtttgcg gcgacaacgg cgagcgctac ccctgggttc tcatggtacg
     5161 cggtgagagg cgccaggggc tatcggctgg aagtgaaacc agttgtggag gagggcaaca
     5221 ccgccacgcg tgaatactcc gtatacctgg gcacggtaac cagccacgtt atccccgcca
     5281 tcaaagcgct ggacccgggg gcgtatcagt ggcggctgcg ggtgtacacc gcggcgggca
     5341 gcgaggaaac cccctggagg ctgctggtgg tgctgcctcc cgcgccggta ttggacaccc
     5401 cggacaatgg ttccgttcat cccggcgtaa cggtagagct taactggttc ccggcagcgg
     5461 gagcggcacg ctatgaaatt caggcggata ataacaacaa ttttagaacg cccgaaatcg
     5521 attatgaatt cacagaaggt ccggttacga ccaaagaggt aacttttcct ttcgctggcg
     5581 ggttttactg gcgcgtgagg gcgtataact acctgggcaa cccgggcgcg tggagcgcgg
     5641 cgaggtattt acgactcaca acaccctgaa ggcaaaacgc taattaacca gcgtcagatg
     5701 tgatgatacg agagggtccc agcgcggttt tccggcggga ccctttgctt atccttgaac
     5761 tgtttggggt aataaacccg tctgcgttaa aaggcctgcg gcaatgagcg gtttaaggac
     5821 caactgttcc ccaggcgctg gttttggtgg gagagggagt gggcgtgatg ctgcctgtgg
     5881 gtgtggctgt gcccggggtg gtggtgaagg agggcgtttt ggtgagggtg gctgtcgctc
     5941 tcacgatggt aggcgtgatg gttgggcttg gcagcgaagt agaatagacc atcacgggat
     6001 gtgtgtctcc accggagatg tccattacat aattcactgc cgcgcggcac ccgccttcaa
     6061 ggttggcggc cacgtgcagc caatccagcc ggttgcccgc ttcccaggtg cgcttcaaag
     6121 cgtgccacag gcagccagga ccagcgttgt aattgaccag cgtgtagcgc cacaggtcat
     6181 cgtgagaggc tacagcgccg gctgaagccc cggtgaggtt atacagcatg cggtccacct
     6241 gggcgcaatt gcccacgatc gtttcgccaa atatttttac gctgaaattg gcttgggtca
     6301 ggtcgattcc cagatcgcaa tccgggcagg tggcattggt tttttgtacc agcgcccccc
     6361 gcagcgttgt ctgctgttcc tggctgaggt taccataacc ccgggcgcag gtatcttcgt
     6421 aaagaacgag agggcagaac tgagtataaa attcagggtt ccacagcaac gcggcttccg
     6481 ccccgtcttc tgtgagctga ccaaacccca cctcttccgc gttatggtag atgccgggcc
     6541 aaagctgact ttcgtgggca aaaatatttt ttaacaggat ggaaggaatg ccggtctcga
     6601 ttgacgtacg gatgatctca tcatcaaact ggttttgcca caaggttacc gcttgcatgg
     6661 cggcttccat gccgcactgg ttggcggaag cgtagtaatc caacccgccg tacgggcaat
     6721 cgccagcgtc gaccacgcca ttctgaatca gcatggcggc caggtaatgc agcggcagcg
     6781 aagtggacaa actagcacga tcagccgggt ttttcagcca ggcaggaagc ccctccacag
     6841 aggggaagga ctgccatatc gccgcgcagg aggacatttg ctgcgcctgc cattgagagc
     6901 tgatgatatc gacgtaatac cctgagcgct ccggcgcgcc agcgttgtcg gctggcatat
     6961 ccgcccacgg cagcactcga atataggcgg tgtactcctc tgtggtgtcg ccataactgg
     7021 attctcccca gaaggtcaac atcactccct gctcgcctgt ttgccatagc ggcagcggac
     7081 agcggctgcc tgcgcaagag aatttggttc ccccgtaaac gccatgaatg cgggtgattg
     7141 attcgttggc gagtttctct tcgccgatga gcacgacctc cggtcgccct tcccacttgt
     7201 tggttgtgcc gggcaagaca gagccattta ccgtgatcca cacgacggga ggaggcaggt
     7261 tgaccatgat ctcgtttgcg gagagcgaag aacgcgtatt ttgcaggtac aaaccgctgc
     7321 atgaactgag gtcctttccc tcttctccgg cagtgcaggg cggggtggtg agccaggtgt
     7381 ttaacacctg gtcaccgcag aacttttgta tctccagttc cgagggggtg ccagcgtggg
     7441 cagtcgtgaa ctggcagacg atggtgttgt cactccagcg agccagccac caatcgtatt
     7501 ggctgtcgtc caccgttatc caggtaaccc ggtcgggtcc atcctgagct gaggcagggt
     7561 gaatattcac cgagttggta atcaacaatg ccaccgtcag caccgtcagg caaacgagca
     7621 atatcccttt accggttaaa tatgaaaacg cccctcggac acgcatgatg attcaaattt
     7681 ttccttgatg aaaaggatag catagatcgg tgcttaagta aatgaattta caatctcgca
     7741 aaatcgcata atgaacttat aatcatagta aaagatttac aaatttctaa gattgagagt
     7801 aaaaatgcaa gagagagccc aggggggtct tttggaaccc caaacctatt ctatctcggg
     7861 tagtgatttg gtggcgttaa tggaggcggt actggcaaaa aatggaactt ttcgcttcac
     7921 cgccagcggc agcagcatgg cgccgttcat tcaaacaggc gacatggtca caatctcctc
     7981 actgcataag ggaagggtag gattgggcac ggttgtggct ttcaaacacc ctgtgggcgg
     8041 gcacctcgtg attcaccgcg ttgtgggcgt ggcgcatggc acttttttga taaagggcga
     8101 taatacaccc catcgatctg atggctggat cccgtcaggc tgtttgatcg gccgcgtctt
     8161 agaaatcacc aggaatggca ggcgcgtatg gctcgggttg ggtgttgaaa ggtatttaat
     8221 cgcctttttt tcgcgctggc aatggttgat cccgctgaga ggcgcgctcg ccgcgctgcg
     8281 ccgcaggtag aaccccgaac catgggcctg ataccgagat cgttcgctgg ttatcttcaa
     8341 ggttgaggga agcaggcaag agagtgtggt ccatcatcat taagaaagtg aattatactt
     8401 aactaccaat agcagaaagg gtggaagacc ttcatggata atgaacttga agaacgcagc
     8461 agcctcgaaa gcattttaga ttacgcgcaa accttgtggc gctgggcttg gctgcttctg
     8521 atcgtggcaa tcgccgccgg cgcggttgcg ttttacctta ccaatcaaaa accacgcgta
     8581 tatgaatctt caaccagagc ggtcgtcaat gtcgtcaccg gttcgaattt ttacgacgcc
     8641 tattcggctt cgtttggcgc tcaacgcctg gctgagacgt attcacagac gatgatcaca
     8701 cctgagctgc tgcaatcagt gtcagaacgg cttggttttg aagtgaccgg caaaatcacc
     8761 gtgacccccg tggagaattc cccgattttt accattgttg tcacagacaa cgacccgcaa
     8821 aaagcagcgg acaccgccaa tatgctgatt accatcttcg ccgagaaggt catgaaggat
     8881 caatcttccc gctattccga gctgaaacag ggcctggaag aggaaattgc ccgcattgac
     8941 caaaatctga ctgatataaa tgagagactg gctattttgc agatcaaaga ggctgaactg
     9001 gcagaggctg aaaaacgcgc cgcccagatg gctgctgaag ggcttgaacc tattgaggtt
     9061 gtgcgcgacc cgaatgatta tgtgacccgt gcacaactgg aactggcgca atcccaatat
     9121 cagtcaactc gttcatcact attctacaat ttacaacaaa ttaaattaac cgaggttcaa
     9181 tctgccacta ccatcagtca gatcgacaaa gcgatggcta atccagaacc catacagccg
     9241 caaccgttta agagcgccgg tttggcggcg atcgtggggt tgatggtagc cgcgggaatt
     9301 gttttcttga tcgctttttt gcaggatgaa atccgggacc cggaagagtt tgcgcgccga
     9361 tggggggtgc cggtggtggg tgtgattacc cactacccgt ccaaccagga caagttgatc
     9421 acggttgcca aaccgcgtat gtcggtgtca gaagcctttc gttcactgcg caccaacctt
     9481 caattcagcg gcatcgacac ccctttgaaa tcggtgctgg taacaagccc atcgccgtca
     9541 gaaggaaaat cttcggtcgt ggccaacctg gcgacggtga ttgcccagag cgagaaggaa
     9601 gttgtgttag tggatggaga tttacgccga cccacgctgc acaaggtgtt caaattatcc
     9661 aaccgcatcg gtctttcgga ttactttatc cgcgaacctg aaaaaatgtc tggcgtggta
     9721 aagaaaacga aagtgaatgg gctgaacata atctcatccg gcagcctccc gcccaaccct
     9781 tccgagcttt tgggttcaac caaaatgcac gatgtattag gtttgctgga gaagcatttt
     9841 gatatggtgc tgctcgactc tcctccgctg ctggcggtaa cagacgcgct gatcctggcg
     9901 aaaagtgtgg atggcgttct gctggtggtt gacccgaaaa agaccaagcg cggggccatc
     9961 cgccaggcca ttgaacagct gcagcgcctg gacgccaggt tgttaggggt ggttctgaac
    10021 aatatcaaaa taaaacgatc gagttattat tacaaccgcg attactacta tagcaaacag
    10081 tacggcaagt ctaacgagga cggtactcac gaagaaccag cggtcgaaga ggataaaacg
    10141 cggtagaggg cccaatcaca aatgaccggc gagccgaacc cagcggtgaa agggacgaaa
    10201 acggacaaaa agagaaacga cacactcttt tgtcctttcc tcggttttga ggatgatccg
    10261 acaaccgcgc tggcgtaccc cgccaagctc aatttttgtt ttcgcgctaa acccgccgcc
    10321 ccggtcagcc tggcgcacca acgcgataaa tgcctgaccg agttctatgt ggattgcccc
    10381 gtttttcaaa gtgaaaaccc gatcccgctg ccagagagca tccggggtga acggaaatcg
    10441 aaaacttttc agttcaactg ggtgccccta gttgctttaa tagcagtagt catcatcggt
    10501 ctcgtagctc tgctgctcac cggggtgttt acttttcgtg gattcacacc gccgctggct
    10561 gacatttcga ccgcgactca cacgcctgca cacctaccag tggtgccgct ttctgcagaa
    10621 ccgaccacta ctgttgaagc aactgccaca ctggtgccaa cagtgaccgc tacctggttg
    10681 atcccgggta agcacacacc gcgcgcgttg gaaacgccgt tcggcacttc ccctcaattg
    10741 gtgatccaca aggtcgccgc gggagaaggg tttattttac tggcagaaaa atttcatact
    10801 accgctgacg cgatcaaggc catcaattac caacttccgg attcattatg ggagaatacc
    10861 ctgctggtga taccggtcaa caccgatgat gttgcgggat tgccagcttt cagcgtggtt
    10921 gagatacgcg cggaagggat gacgattgaa tcttatgccg cccggatgca gctggatgtg
    10981 gaaatgttga aaaagtacaa cgatctgcct gaaggttatt cgtttcaaat tggcgaaatg
    11041 ctgatcgtac cgatgacaga atgatcaaca tgggtagaga gtgaaccatg ccgaatgtcc
    11101 tttttgtatg cagcgccaac cggttccgtt ctgtactggc agctgaatat tttacatctc
    11161 tgcttgaaaa acaagcaccg agcggcgaat gggttgtggg cagcgcgggt atttgggcaa
    11221 gagaaggcgt ggctcccacg aaagaagcgc ttcaatgggc ggcgggtaaa cagcttcata
    11281 ttgagtctgt gcgatccaga gagatatcaa acgcgctcat tgtcgaggcg gacttgatca
    11341 tcgtgatgac caacggtcag cgggaagcca tttctatcga gtttcccgag gcgagagaaa
    11401 aactcaccct gctctctgaa atagccgccg ctcaaatcta tgatattcca gacccaatca
    11461 tagaaagcgg tgaagaaccg ctacagatcg cggaagaaat ttccacgctg attgacgccg
    11521 ggtttgagcg aattctcaag cgggcggctg ggcgagccaa taacctggga gcgcagcttc
    11581 gtggagcata aaccgcctaa cgcgaaaaga cgcctgctgt ggctgttatt ggtattattg
    11641 ttcctggtaa gctgcggata tttgctctgg acaaaccccc ttctgggcaa gcggcttccg
    11701 caattcacct atgagccgtt ggccacattt accgccatcc ccaccagcgc cgcgcttact
    11761 ctcacacctg aaacggttaa aacagaaccc gcgctggcga ctgttgagcc tgaacccaca
    11821 agcgtttcac ctgtttgtgg gcaaacccag ccaatgataa tcctggcttt gggcattgat
    11881 gaatcagaac aggcggatat gatcagggtt gtgagagtgg attggctgaa gcaccaggtt
    11941 gtggtgttat ccatcccacg cgatttctgg gttccaatac cggggctgga ggagcataat
    12001 atcagccagt ttcgcattaa cgcggcttat ggctacggcg agtacttcaa cgggcgtggt
    12061 cagggcgtgg tgaagttttc tgaaaccatg taccagaact atggcgttac atttgaccgg
    12121 tatgtggtgg cgcattttaa tacttttaca gaactggttg accaaatagg gggagttgac
    12181 atcgaccttg aagaacccat tggggcgtat gcgagcgcgg gtcatcatca ctttgatggc
    12241 aaatcagccc tgcttttcgt caggcaaagg atagcggatc tagacgcgta tcgtatccag
    12301 cggcagtcag ccattttaaa gggattgtac gaaaaacttt ctaaacctga atatttagtc
    12361 caactccctg ggctgggagt gaaatttatt aaggataagt cggtcatcac tgacttacgc
    12421 ttgcaggatg tatatacatt caattgtttc atccgtgaaa tcaaccgtga ttctttggtt
    12481 tttattgata ttccttatga cctgtacacc cctatgatca ccaattatgg ccgacatatc
    12541 aaactgccca agccagaggc tggggtcttt atccaaaatg tgatcaaaaa cggaactgaa
    12601 taaccttgcc ggtacctccc ggaggcaata ggcggaaaag tttttcccgg acagattaag
    12661 tcaaaataca tgcaaacact tcttaccggc tggcaatttt cttgttctct gcccaggtgg
    12721 ctccaaggtc taactcagac gacgcgtaag agaaaaacct caaagaaatg cttctgctgc
    12781 agcccgacca tttaccagga aaagaaacgc aaaacgctac tgggtagcgc acggcggcgc
    12841 tcttttcggt atccttatct ttctataaca ggagtaataa ggtataatat atcttaatat
    12901 attgataaga atacagcgat caaggaaatg aaaccatgag cgaactgatc aacaataagc
    12961 agaaacgagt tgagttaatg aagtctctta tccggcaact tcaccagggc atggcagaag
    13021 accgtgtacg catgcagctg gaaaccctgc tggacgaagc cgattacaac gatgtgtttg
    13081 taatggaggt acaactgatt caagagggta taccggcgga ggttgttcag gatttgtgtg
    13141 atacgcatac ccgtgtattg aaaaaacacc tggacctgca agaaacgccg caaaccatac
    13201 ctgggcaccc tgtacacacc tttgttgaag aaaaccgtga attgaccaaa gtgaccgcgc
    13261 agatcaggca cctgatgagc ctgatagccg aaaagcccgt tgaagaagac gtgagcgagc
    13321 aaatgggcga aattcaccag ttgctcaata acctgatgga cgtggaaaaa cattatcaac
    13381 gcaaagaaaa cctgctcttc ccgtattttg aaaaaaacaa ccttcccggc cccccagcgg
    13441 tgatgtgggg gaagcatgac gaagtcaggg aattgttaaa aaccacactt gaggggtttc
    13501 aacaggtgaa gtccttttca gcgggagagg ccgcggcttt taaccagttc agtgtgagca
    13561 tggtggtgga cgcgattgat gagatgatat acaaagagga aaagatcctc ctgccgaccg
    13621 cccttgatat tttaagtgaa gaggattggt acgccatcta cctggaaagc gacgagggtg
    13681 gttattgcct gtacgttccg caattcacct ggacgccaga aggtgatttt aaaagggaaa
    13741 taagaaatcc atctggagtg ggcgctcgaa tacaaatgcc aaccggcagc ttcactttgg
    13801 atgaactggt tgccttgttc tccactttgc cttttgacct cacttttgtg gacaaggacg
    13861 atagcgtgcg atatttcagc cctggaacag aaaggatttt taagcgtacg cgcgctatcc
    13921 tgggtcgcaa agtccagtat tgccacccgc cgaaaagcgt gcatatcgtc aaccgaattg
    13981 tgaaggactt caaagaaggg aaacaggacc aggcgcgctt ctggatcaat aagggcgggc
    14041 gcatggtcta tattgtctat tatgcggtgc gcgatgagca aggaggttac cttggcacat
    14101 tggaggtaac
//
LOCUS       DAOSPM010000046        14072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_4353_length_14072_cov_14.738246, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000046 DAOSPM010000000
VERSION     DAOSPM010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14072
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4353_length_14072_cov_14.738246"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLW25_05500"
     CDS             complement(<1..600)
                     /locus_tag="PLW25_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOS53979.1"
                     /translation="MFIPETEILIRLGLAALLGMMVGYERERQNQPAGLRTHVILAVG
                     SCLAMTVSIDLAIQFYPHAPNGDPARLAAQVISGIGFLGAGAILRYGTNVKGLTTATS
                     LWAIAIVGLAVGAGHYFSAVGTTLAMLIVLTILNIIEKRYIRAYQIITVAVTARENPA
                     LVETLLEVFKKLKKKVLNIGFEADPIKKSLTVNLVVKTLE"
     gene            complement(616..2046)
                     /locus_tag="PLW25_05505"
     CDS             complement(616..2046)
                     /locus_tag="PLW25_05505"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOS53980.1"
                     /translation="MQPKRQIYLLDPQQLSPETIAVTFAKTSRSPESFRDIAAELTAA
                     SSADFNQKWVVGYGHSSVAEHAVLHIALENVSRLAVENIESNRLASYTEKSTRYQTWE
                     DDAFHLPAELTGHPLEGLFTSACATLFSIYRSCIQAVIAHLKSNSQSQAGESEAQLNR
                     RLRSQAIDACRFLLPAASLANVGVTINARALEHAVGKLLTSPLIEAQQIGAEIRHAAS
                     ASIPTLLKYADQIPYLQHVCQQFSALSIQVDAAPEPDWCQLIGPQPDVLEKILAAELY
                     REQDMSFTQALKAIRTLPDHQKNTLLRCLLETPDRHTIPSRELEYAFFTFDLLIDQGA
                     YFEVKRHRMMTQTAQLLTARFGYAIPALITEAGMDAAYCAAMEKAREVYDQLADFNPD
                     VAAYIVPNGFNRRVLLHLNLRSLVHFIRLRSAPNAHFSVRRVAQRMAEEISAHFPELS
                     PLLGRNENETLKNIETTFFTNTHVVS"
     gene            complement(2036..2965)
                     /locus_tag="PLW25_05510"
     CDS             complement(2036..2965)
                     /locus_tag="PLW25_05510"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOS53981.1"
                     /translation="MNYIEALQQANLKESWATIGAFDGVHIGHRALFQKLVDGARQDG
                     CKAVAITFEPLPALFFNRIKNGQVLTTLAQRVSLIKALGIDEVIVLDFDQALADVEAF
                     PFLQQVHKAIGLRRLLSGFNSRIGKDQAGAVPKLQEIGQVLGFCVEVVPPVKNGQDII
                     SSSNIRKLLKAGEITRANQFLGRPYALIGEVVHGEHRGSKLGIPTANLRLPAEQMLPA
                     SGVYACRASVGELAFIAVTNVGIRPTFENPLPVPRVEPHLLDTSETFYGVNLELEFFA
                     FLRPEIKFPDAKALVAQIQLDIQKTREYFANAA"
     gene            complement(3040..3951)
                     /gene="truB"
                     /locus_tag="PLW25_05515"
     CDS             complement(3040..3951)
                     /gene="truB"
                     /locus_tag="PLW25_05515"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOS53982.1"
                     /translation="MENNSGIDVKNAISGVLVVDKPVGMTSHDVVQIIRKGSTIKRAG
                     HTGTLDPRASGVLVVLLGPAVRLSEYVSASDKRYQAVIMLGTTTDTYDADGRTTSSAP
                     VNISEEEFNNELQKFVGQIEQVPPPYSAVKVHGQKAYDIARRGEEVELAPRIIHVYSL
                     ELLEWAPPEVVIDVYCSSGTYVRSLVNDLGSRLGCGATLSGLRRTKSGRFTLRDAVPL
                     RKLNEAFADGSWYQYLIPAAEALSDWPMIELNDQEVDSIKHGIRIPAVDAKQQKVCGV
                     SGQGELVALLELDAEKMEWQPKKVFFS"
     gene            complement(3941..4942)
                     /locus_tag="PLW25_05520"
     CDS             complement(3941..4942)
                     /locus_tag="PLW25_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOS53983.1"
                     /translation="MIDRQIKELLHRSKKIGLTSHIRPDGDAIGAVLGLGLALTAAGK
                     RVEMVLRNGISKTFRHLPGAERIQSAFKTENDLYIVLDCSDLERTGGVLNGREVDIVI
                     DHHITNKGFGKVNLIEPESVATCATLAQHMPDWGLVIDKAVASALLSGVISDSIGFRT
                     SNTTALSLRIAADLIEKGADINQLYNRALISRPFTSVHYWGYGLAKLERENGLVWTTL
                     SLADRHNAGYQEDDDADLTNILSSIEDMDVVVLFIEQKPDQVKVSWRARPGINVASIA
                     DSLGGGGHPAAAGVEMEGNLAQVKDLILSKTRDYLRSLKPENGSPRDSEVTEHINGK"
     gene            complement(4939..5310)
                     /gene="rbfA"
                     /locus_tag="PLW25_05525"
     CDS             complement(4939..5310)
                     /gene="rbfA"
                     /locus_tag="PLW25_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOS53984.1"
                     /translation="MPSPLRLTRIGDRFRKELSEMLVKEEIRDPRLAGIYITDVRVDR
                     ELSYADIYVSAVEGASRSKEVLEGLESASGFIRKALSDRIQLRSFPRLRFHWDITPER
                     ADRIEQLLASIREEERQQKKK"
     gene            complement(5336..7111)
                     /gene="infB"
                     /locus_tag="PLW25_05530"
     CDS             complement(5336..7111)
                     /gene="infB"
                     /locus_tag="PLW25_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOS53985.1"
                     /translation="MSEKTIEFPSQITVRDLAQRMEASPIQVIKILMSNGVMANINQQ
                     VDFDTAAVVASELGFEAVLESVKETAPAEDPGKVPLWRELIDSEDKSKLINRPPVVTI
                     LGHVDHGKTTLLDAIRHANVASGEAGGITQHIGAYQVEHKGKVITFLDTPGHAAFSAM
                     RARGAQGADIVILVVAADDGVMPQTREAIAHAKAARVPIIVALNKIDRADANPDFVKR
                     QLSEQGLVPDEWDGNTIVVPVSAKQKKGLDDILEAVLLVSESLDIKANPHGKVFGTVI
                     EAQVDKARGVIATLLVQNGTLQTGNTVIVGDTCGKIRAMYDFNGKTVKSAFPSTPIQV
                     LGIGNVPPAGELFQVVPSEKEARLILAERAASKENGSETSTRTSLEKLFEKFQAGEVR
                     ELPLILKVDVQGSVEPIVNSLNELSQGEIKINVIHAETGNISESDVMLAAASRAILLG
                     FNVSADQAARNLAEQEGISIRLYNIIYRMIEDIEKALKGMLEPETREIPVGTAQVRAV
                     FKISKVGTIAGCRVLTGELRRNARIRLLREGETLFDGEISSLKHEKDDVKEVRQGFEC
                     GVSLKGYNDINEGDLLENYIIEKSA"
     gene            complement(7215..7544)
                     /locus_tag="PLW25_05535"
     CDS             complement(7215..7544)
                     /locus_tag="PLW25_05535"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOS53986.1"
                     /translation="MAKKPQKRIKHLPQRTCIGCKTVLAKRELVRLVRTPEGIMVDET
                     GKAAGRGAYVHNTRRCWEQALKGPISHALRTDITPADQQRLKEYLERLPEVDPIENRA
                     ESRDVTN"
     gene            complement(7562..9475)
                     /gene="nusA"
                     /locus_tag="PLW25_05540"
     CDS             complement(7562..9475)
                     /gene="nusA"
                     /locus_tag="PLW25_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOS53987.1"
                     /translation="MKNEFTLAFNEVLEDKGLPKEVILEALESAMVSAYRKSVNASNE
                     QPIEAKIDLETGKVTIYAQKEVVEVVENNITEVELETARKVEPEAQLGDLVVVESTPG
                     DFGRVAATTARQVIQQRIRDAERKAQISYYEKQLGEIVSGVVQAIGAAGLTIGLELKT
                     EATMPRKEMITNEHFHIHERVRALVADVKDSPRGPQIILSRTHRDFLRRLLENEVPEI
                     FHGIVEIRSIAREPGDRAKVAVSATQQGIDPVGACVGIRGVRIQAIVKELHDEKIDVI
                     EWNPDPAVFIAKAISPARVSGVYLTDQHSGNAKTATVVVPEDQLSLAIGRIGQNARLA
                     AKLTGWRIDIKSLPEAAADALQKLATDPAVEALKESEHDNALRIEELLARKEEGKPLS
                     PEEYDQMAKFVDRVERKSIAKSEEQRKQELEKLAEIKKQIPASAFELNILDSGLPEHV
                     AVILQEAGYFSVGDLAMQVKQDPDLILGINGIGIRAMESIHELMAALDKQLADEQAAL
                     VKQAEETAEEMEVLEPVSEIELPAEVDVVPSVEVEPVLDVTVGETSSTETIEEPEPEK
                     EPSLEELFTLRPEVLDTAGLLDDDEAEESGKAKKKGKKKGKSVVVTYDPDRDLTVVQK
                     KHKRGGNDWDWDE"
     gene            complement(9841..11130)
                     /locus_tag="PLW25_05545"
     CDS             complement(9841..11130)
                     /locus_tag="PLW25_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HOS53988.1"
                     /translation="MTEALLQLEHVFQSYGAGVRRFTAVENVNLNLSVGEFVALLGPS
                     GCGKSTLLRIIAGLQKATQGKVLYRGQQLEGVNPHTSIIFQTFALFPWLTVLENVSLA
                     LKAKGVEKHERDKKAEDLLDRVGLDGFESAYPRELSGGMRQKVGFARAMAVEPELLCM
                     DEPFSALDVLSAESLRGELLELWTEGKIPTKAILMVSHNIEEAVFLADRIVVMDKEPG
                     RIIAEHKIDLPHPRNRKSVEFQNHVDQVYAVLAGQTRPEKEELGTAPGEVGITRHLPE
                     VEISDLAGLLENLAEGGNQKHDIYLLEDKLGITSDQLLKLTEAAELLGFSSIAKGDIE
                     LTALGQAFADASILARKEIFASCVRRIPLMRWLLSMLHSAEDRRLKIEVILTALSLDF
                     STEEAEKQIEVLIKWGRYGELFVYDDDQEMIELEEGR"
     gene            complement(11133..12857)
                     /locus_tag="PLW25_05550"
     CDS             complement(11133..12857)
                     /locus_tag="PLW25_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOS53989.1"
                     /translation="MTQNTRVFGTSQNSRKPLTWGDIIALSLVIALLYVGARLAVNAP
                     EVVPGPDISLSPSALPIYTLFSVGRMAAAYLLSFLFSIIYGRVAAYHQNAEKILMPLL
                     DVLQSVPILSFLPVVLLSLSSFLPEGTAAEIASIVLIFTSQAWNITFAWYQSLTTIPK
                     ELKEASSIFRLNKWINFKALELPFGMISLIWNSMMSWAGGWFFLMAAEIFTVGSRDFR
                     LPGLGAYLQEAANQSNHVAIFLGTGVLIFTVVLLDQLIWRPLIAWSDRFKVEMVENDN
                     PPTSWFLDLMHNANITRIISNRIFAPLTEWFDRLTIKHAPPVSRLENGKNQLKWYVIV
                     FSIIVFALISYGLYHATEMLLSVPVQKWGVIGLGVTATFLRVIVALLIALAWTIPVGV
                     LIGTNRPLATVLQPIVQVTASVPATALFPVLLLLFLNAQGGLNTAAVLLMLMGTQWYL
                     LFNVIAGASAIPQDLIFTSKLMGLSGWQRWRTLLLPAIFPYLVTGAITASGGAWNASI
                     VAEYQKFGEKTHTVTGIGSLIAQATASGDYPLLLAGTLAMIITVVVVNRVIWRRLYRL
                     AEEKYRME"
     gene            13180..>14072
                     /locus_tag="PLW25_05555"
     CDS             13180..>14072
                     /locus_tag="PLW25_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOS53990.1"
                     /translation="MITDTRQNYRVLLPGLLLGLAVFALLISLSSIHQVFTLFESFKW
                     VYFAVAVGLNIIHHALRFLNLSFSLNISGIRRIPLKKRMAFYLSSSALTAADPKAQES
                     YRSVWLSKACGIPLVRIDSIFLIDRLSDGLSILALATLGILAYPAFWPFFAAMLLLFV
                     LSILFLQIKPAGEGLLDAGEKLPLLRLFSRQLRQCMEGNEHLFRTFPMTVSLFFNVLA
                     WIALAAALFFVLVGFGLEPSLTLAAIACLVLAFSIIIGFFSTVPGGLGVVELALAGLL
                     TVLLGFQPELAVTATILFRMST"
BASE COUNT         3176 a         3703 c         3553 g         3640 t
ORIGIN      
        1 ttcaagcgtt tttaccacca agttcaccgt caggcttttc tttatcgggt ctgcttcaaa
       61 gcctatgtta aggacttttt tcttaagctt tttaaaaacc tcaagaaggg tttccaccaa
      121 cgctggattt tcgcgcgcgg ttaccgccac ggtaatgatc tggtaagcgc ggatataacg
      181 cttttcgatg atgttcagga tggtcagtac aatgagcatc gccagggttg ttcccactgc
      241 ggaaaaataa tgcccggcgc cgacagccag cccaacaatg gcaatggccc acaaagaggt
      301 ggcggtggtc aatcccttga cattggtacc gtagcgcagg atggcgcccg ccccaaggaa
      361 accgattcct gaaatcacct gggctgccag acgcgcaggg tccccgttgg gcgcatgcgg
      421 atagaactgg atggccaggt cgattgaaac ggtcatggca aggcaggatc ccactgccag
      481 aatgacatgg gtacgtaacc cagccggctg attctgtcgc tctctctcgt accccaccat
      541 catacccagc aaagccgcaa gaccaagccg gatgaggatc tcagtttctg gaataaacat
      601 gatggttctc cttccttaac ttaccacatg agtgttggta aagaaagttg tttcgatgtt
      661 ttttaatgtc tcgttttcgt ttctgcccag caagggagac agttcgggga agtgagcgct
      721 gatttcttct gccatacgct gcgcgactcg ccgcactgag aaatgagcat tgggggcgct
      781 gcgcagtcga ataaagtgaa ccaggctgcg caggttaagg tgcagcagta cccggcggtt
      841 gaagccattt ggaacgatgt aagcggccac gtcagggttg aagtctgcca gttgatcgta
      901 aacttcacgg gctttttcca tcgcagcgca ataagcggcg tccatgcccg cctcagtgat
      961 aagcgcgggg atggcatagc cgaacctggc ggtgagaagc tgcgctgttt gggtcatcat
     1021 gcggtgacgt ttcacttcaa agtaggcacc ctggtcaatc agcaggtcaa aagtgaaaaa
     1081 cgcgtattcc agttcgcggg aagggatggt atgacggtct ggcgtctcca gcaggcagcg
     1141 caacaacgtg ttcttttgat ggtcaggcaa tgtacgaatg gctttcaatg cctgcgtgaa
     1201 actcatgtcc tgttcgcggt ataactcagc tgccaggatt ttttctaaca catcgggttg
     1261 cggaccgatc aactggcacc aatcaggttc tggggcggca tccacctgga tcgataaggc
     1321 agaaaattgc tggcagacat gctgtaaata cgggatctgg tccgcgtatt tcagcagggt
     1381 ggggatggat gcagaagcgg catggcgaat ttcggcgccg atttgctgcg cttcaatcaa
     1441 tggactggtg agcagttttc ccactgcgtg ttctaacgcg cgcgcgttga tggttacgcc
     1501 cacattggcc agcgaggcgg ctggaagcaa aaaacggcag gcgtcaatcg cctgtgagcg
     1561 taaccggcga ttcagttgtg cttcgctttc gcctgcctgc gattgggaat tgctcttcag
     1621 gtgggcgata accgcttgaa tacaggagcg atagatggaa aagagcgtcg cgcaggcgct
     1681 ggtgaacaag ccttccaacg ggtgaccggt caattcggcg ggtagatgaa aggcgtcatc
     1741 ttcccaggtt tgatagcggg tagatttttc tgtataggaa gccagacggt tggattcgat
     1801 attttcaacc gccagccgcg agacattctc aagcgcgatg tgcagcacgg catgttctgc
     1861 cacagatgaa tgcccgtaac ccaccaccca tttctgattg aaatccgcgc ttgacgctgc
     1921 ggtcagctca gccgcaatat cgcgaaagga ttcgggtgac cgtgaagttt tggcaaaggt
     1981 cacagcgatg gtctcagggg acaattgttg tggatcgagt aaataaatct gtcgtttagg
     2041 ctgcatttgc gaaatactcc cgggtctttt gaatgtcgag ctgtatctgc gcaaccagcg
     2101 ctttggcatc tgggaacttg atttctgggc gcaggaatgc gaaaaattcg agttccaggt
     2161 tgacgccgta aaacgtttcg ctggtatcga gcagatgcgg ctctacacgc ggaactggta
     2221 atgggttttc aaatgtgggg cggatgccta cattggttac agcaatgaag gccagttctc
     2281 ccacacttgc cctgcaagcg tacacgccac tggctggcag catttgctcc gctggaaggc
     2341 ggagattggc ggtgggaata cctaacttac tgccacggtg ttccccgtgt accacttcac
     2401 ctataagagc ataaggccgc ccaaggaact gattggctcg tgttatttct ccagctttga
     2461 ggagtttacg aatattgctg gaagaaatga tatcctggcc atttttgacg ggcggtacaa
     2521 cttcaacgca gaagcccagt acctgcccaa tctcctgcag tttgggtaca gcaccagcct
     2581 ggtctttgcc aatccgggaa ttgaaacccg agagcaacct ccttaaccca atcgctttgt
     2641 gaacctgctg caaaaaaggg aacgcttcca catctgcaag cgcctggtcg aaatctaaaa
     2701 caatcacctc atcaatcccc agcgctttga tcaatgaaac ccgttgagcg agtgttgtca
     2761 ggacctgacc gttcttgatg cggttaaaga aaagcgctgg caatggctca aaggtaatgg
     2821 caactgcctt gcagccatcc tggcgggcac catcaacgag tttttgaaag agggcgcggt
     2881 gcccgatatg cactccatca aaggcgccga ttgtcgccca ggattccttg aggtttgctt
     2941 gttgtaacgc ttcgatgtag ttcatgcgtt aattatatcc gcatgcgttt ctgctttgga
     3001 tatggcgaga acccgctcga ccaggcgaga gggaagtggt tacgagaaga acactttctt
     3061 gggctgccat tccatttttt ctgcgtcgag ttccagcagc gccaccagtt ctccctgacc
     3121 gctcacgccg caaacttttt gttgtttcgc atcaacagca ggaatgcgga taccgtgctt
     3181 gatagaatcg acctcctggt cgttaagttc gatcatcggc cagtcggata aagcctcggc
     3241 ggctggaatc aggtattgat accacgatcc atcggcaaag gcttcattca gtttacgcag
     3301 gggaaccgcg tctctcagtg tgaagcggcc gctttttgtg cgccgtaaac ctgaaagggt
     3361 agccccgcag cccaaacgcg aaccgaggtc gtttaccagc gaacgcacat aagtgccgga
     3421 ggagcagtac acgtcaatga ccacctcagg cggcgcccac tcaagcagct ccaggctgta
     3481 cacatgtatg atgcgtggtg ccagttcaac ttcctctcct ctgcgcgcta tatcgtaagc
     3541 tttctgaccg tgcactttaa cggctgagta aggcgggggc acctgttcga tctgaccaac
     3601 aaatttttgc aattcgttat tgaattcttc ttcagatatg ttcaccggcg cagatgaggt
     3661 ggttcgtccg tcggcgtcat aggtatcagt ggtggtgccg agcatgatca ccgcctggta
     3721 gcgcttatcc gacgcagata cgtattcgct taagcgcacc gcagggccga gcaacaccac
     3781 cagcacgcca gacgcgcgcg ggtcaagcgt accggtatga ccggcgcgtt tgatggtgga
     3841 gcctttacga atgatttgta ccacgtcatg cgaggtcatg cccacgggct tatccacaac
     3901 caatacaccc gagatggcat tcttaacatc gatccctgag ttattttcca tttatgtgct
     3961 ccgtcacttc gctatcccgg ggtgaaccgt tttctggttt cagagagcga agatagtctc
     4021 tggttttact taaaatcaaa tcctttacct gtgctaaatt gccttccatt tcaacaccgg
     4081 ctgcggctgg gtgaccgcct cccccaagag aatcggcaat agacgccacg ttgatacccg
     4141 ggcgggcgcg ccaactgact tttacctggt ctggtttttg ttcgataaaa agcaccacca
     4201 cgtccatatc ctcaatgctg gaaagtatgt tggtaaggtc tgcgtcatcg tcttcttgat
     4261 atccggcgtt gtggcggtct gccagtgata gcgttgtcca cacgagcccg ttttctcttt
     4321 caagttttgc cagcccgtat ccccaatagt gaacgctggt gaaaggacgg ctgataagtg
     4381 ccctattata caactggtta atgtcagcgc ctttttcgat caggtcagcc gcgatgcgta
     4441 aagataaggc ggttgtgttg gaggtgcgga agccgatcga atcgctgatg acacccgaaa
     4501 ggagagccga cgccaccgct ttgtcaataa ccaatcccca gtcgggcata tgctgcgcca
     4561 gggtggcgca ggttgccacc gattcaggtt cgatcaaatt caccttgccg aaacctttat
     4621 ttgtgatgtg gtgatcgatc acgatatcaa cttcgcgccc gttcaataca cccccggtgc
     4681 gttcgaggtc actgcaatcc aagacgatgt acaggtcgtt ctctgttttg aaggctgatt
     4741 gaattctctc tgccccaggc aggtgtctga aggtttttga aatgccattg cgcagcacca
     4801 tttccacgcg ctttcccgcg gcggtcaaag ccagaccgag tccgagcaca gcaccgattg
     4861 cgtcaccatc cgggcgaata tgagaggtga gccctatctt ttttgagcgg tggagcagtt
     4921 ctttgatttg ccgatcgatc atttcttctt ctgttgtctt tcttcttcac gtatggaagc
     4981 caataactgc tcgatacggt ctgcgcgctc gggggtgata tcccaatgaa aacgcaggcg
     5041 cgggaaggag cgtaattgga ttcggtctga cagcgctttg cggatgaacc cgctggcgct
     5101 ttccaatcct tccagcactt ctttcgatcg cgaagcccct tccaccgctg aaacgtaaat
     5161 atccgcgtaa gacagttcac ggtctacacg gacatccgtg atgtatattc cagccaggcg
     5221 tggatcacgg atctcttctt tgacaagcat ctcggataat tccttgcgga atcgatcgcc
     5281 gatgcgggtt aaacgtaatg gtgaaggcat agcagtacct gtactttagc tcagactaag
     5341 cggatttttc aatgatgtaa ttttccagca ggtcgccttc gttgatatca ttataccctt
     5401 tcaacgatac accgcactca aacccctggc ggacctcttt cacatcgtct ttttcgtgct
     5461 tgagggatga gatctcgccg tcaaacaggg tttcgccttc gcgcaacagg cggatacggg
     5521 cattacgccg cagctctccc gttagcacgc ggcagcccgc gatggtgccc actttgctga
     5581 tcttgaacac ggcacgcacc tgggcggttc ctacgggaat ttcgcgggtc tcgggttcca
     5641 gcataccttt aagcgctttc tcgatgtctt cgatcatacg gtagatgatg ttgtacaaac
     5701 ggatggaaat gccttcctgt tcggccaggt tgcgggcagc ctggtcggcg ctgacattga
     5761 atcccagtaa gatggcgcgg gaagcggctg ccaacatcac atcgctttcg ctgatgttcc
     5821 ccgtttcagc atggatcacg ttaattttga tctcaccctg gcttaattcg ttcagagagt
     5881 tgacaatcgg ctccaccgaa ccctgaacat ccaccttgag aatcaaagga agttcacgaa
     5941 cctcgccagc ctggaatttt tcaaataatt tctcaagtga ggtgcgagtg gaggtttcag
     6001 atccgttttc tttgctggcg gcgcgctctg ccaatatcag gcgggcttcc ttttcacttg
     6061 gaaccacctg gaacaattca ccggcgggtg ggacattgcc gatccccaat acctggatgg
     6121 gtgttgaagg aaaggcgctt tttaccgttt tcccattgaa atcatacatg gcgcgaatct
     6181 tcccacaggt gtcacctaca atcaccgtgt taccggtctg taacgttcca ttctgaacca
     6241 gcagggtggc gattacccca cgtgctttat ccacctgcgc ttcgatcacg gtgccgaaaa
     6301 ctttcccgtg cgggttggcc ttgatatcca ggctctcact caccagaaga accgcctcca
     6361 ggatatcatc taatcctttc ttctgttttg ctgacactgg caccacaatg gtgtttccgt
     6421 cccattcatc gggcaccaat ccctgctcgg agagttgacg tttcacaaaa tcggggttgg
     6481 cgtccgcgcg gtctatctta ttgagcgcca cgatgatggg caccctggcc gccttggcgt
     6541 gggcgatcgc ctcgcgcgtt tgaggcatca ccccgtcatc tgcagccaca accaggatga
     6601 cgatatcagc gccctgggcg ccgcgtgcgc gcatggcgga gaaagccgcg tgcccgggcg
     6661 tatccagaaa tgtgatcact ttccccttgt gttctacctg gtatgcgcca atatgctggg
     6721 taatgccacc ggcttctccg ctggccacat tggcgtggcg gatggcatcc aggagggtgg
     6781 ttttaccgtg gtccacatgc ccaagaatgg tgacgaccgg agggcggttg atcaatttcg
     6841 atttgtcttc gctatcgatc agctcgcgcc acaggggcac cttcccaggg tcttcagccg
     6901 gcgcggtttc tttaactgat tcgagaacag cctcaaaacc cagttcagaa gccacaaccg
     6961 ccgcggtatc aaaatctacc tgctgattga tgttggccat cacgccgttt gacatgagga
     7021 tcttgataac ctgaattggg ctggcttcca ttctttgcgc caggtccctt acagtgattt
     7081 gtgagggaaa ttcgatcgtt ttttcgctca taacacacct ccaaagttgt tccccctaca
     7141 atctgtcatg taatctgttt atgttcacca attcctgaaa gggcggtgga tgtgcctgga
     7201 gcggttttct tgaatcagtt ggttacatct ctgctctcag cacgattttc tattgggtca
     7261 acctctggca gccgttcaag gtactctttc aaccgttgct ggtctgcggg ggtgatgtct
     7321 gtccgcagcg cgtggctgat gggacctttt aacgcctgct cccagcaacg ccgggtattg
     7381 tgcacatagg cccctctgcc tgcggctttc ccggtttcgt ccaccataat accctctggc
     7441 gtgcgcacca accgcaccag ttcccgtttg gcgagaaccg ttttacagcc aatacaggtg
     7501 cgttgtggga ggtgtttgat ccgtttttga ggtttcttag ccacctgtct ttctcacgtt
     7561 tttactcatc ccaatcccaa tcattaccac cacgtttgtg tttcttttgt accacggtca
     7621 gatctcgatc ggggtcatac gttaccacaa cgctcttgcc cttctttttc ccttttttct
     7681 tggctttacc gctctcttcc gcttcatcgt catccagcaa gccggcggta tctagcactt
     7741 ctgggcgcag cgtgaacagt tcctcaagag aaggttcctt ctcgggctcc ggttcttcaa
     7801 tggtctcagt tgatgaggtt tcacccacag tgacatcgag caccggttcg acctcgacag
     7861 aggggacaac atcaacttcg gccggcagct caatttcact gacgggttcc aaaacctcca
     7921 tctcctcagc ggtttcttcg gcttgtttga cgagcgcagc ttgttcatca gccagttgct
     7981 tatccagcgc tgccatgagc tcatggatgg attccatcgc gcggataccg atgccgttga
     8041 taccaagaat caagtctggg tcctgcttca cctgcattgc caggtcacca acactgaagt
     8101 aaccggcctc ctggaggatg acagcaacat gttcaggtaa tccgctgtcc agaatgttca
     8161 gttcgaaggc tgacgcgggg atctgtttct tgatttctgc cagtttctca agttcctgtt
     8221 tacgctgctc ctcgctcttg gcaatggatt tccgttcgac acggtctaca aatttcgcca
     8281 tctggtcgta ctcttcagga gaaagcggtt tgccttcctc tttacgcgcc aggagttctt
     8341 cgatgcggag cgcgttgtca tgttcacttt cttttaaagc ctcaactgcg gggtcagtcg
     8401 ccagtttttg cagggcatca gcagcagcct cgggaaggct ttttatatca atacgccaac
     8461 cggttaactt ggccgccagg cgggcgtttt gcccaatgcg cccgatagcc aggcttaact
     8521 ggtcttcagg cacgaccacc gtggctgttt tagcgttacc agaatgctgg tcggtgagat
     8581 acacgccgct gacccgcgcc gggctgatgg ctttggcgat gaatacagcc ggatcaggat
     8641 tccactcgat gacatcaatt ttttcatcgt gcagttcctt gacaattgcc tggatacgca
     8701 cgccgcggat tccgacacag gcgccaaccg ggtctatgcc ctgctgtgtg gcggatactg
     8761 ccaccttggc acggtcaccg ggttcgcggg cgatggaacg tatttcaacg atcccgtgga
     8821 atatctcagg gacttcattt tcaagcaaac ggcgcaagaa atcccggtgg gtgcgtgaga
     8881 ggataatttg cggaccgcgc ggcgaatctt tcacatcagc gacaagcgcg cgtacacgct
     8941 catggatatg gaagtgttcg ttggtaatca tctctttacg gggcatggtg gcctcggttt
     9001 tcaattcgag accgatcgtc aatcccgctg cgccaatcgc ctgcaccacg ccgctgacga
     9061 tctcgccaag ctgtttttcg tagtaactta tctgggcttt gcgctcagcg tctcgaatgc
     9121 gttgctggat gacctgacgg gcggtcgtag cagccacgcg gccaaagtcg cccggggtgg
     9181 attctacgac taccaggtca cccaactggg cttctggctc caccttgcga gcagtctcaa
     9241 gctctacttc ggtgatgtta ttttcaacga cttcaacgac ttccttttgg gcataaatgg
     9301 tcactttgcc ggtctcaagg tcgattttcg cttcgatggg ttgttcgttt gaagcattca
     9361 ctgatttccg gtaagcggaa accatggcag attcaagcgc ttccagtatc acttccttgg
     9421 gcagcccttt atcttcgaga acttcgttga acgccagagt aaactcgttc ttcatttgtt
     9481 attctccacc tgttttcaaa atggtcgata tgaagttttg ataataagaa aagtggggaa
     9541 tgcccacttt tcggtgttga aacctcaaat gtctttcgaa ccgtttctat tttatcacac
     9601 tcttcaaata ctggtcaagg tcaggttgac tttttttgag gaacctcatc ctaaaagcgg
     9661 cgaatccttt ttggattggg gatgctggtg ttccggcagc cgcgtgggct ctccacattt
     9721 tcgtaacgac gctactgaac ccagccgtac cggctaactg agaaccaacc ttcaaagcgc
     9781 tgctctcgcg ttcctttctc actttcgcgg aggatggtga aggcttggtt gcgttttttt
     9841 ttatctcccc tcttcaagtt cgatcatttc ttggtcgtca tcgtaaacga ataattctcc
     9901 ataccttccc cacttgatca ggacttcgat ttgtttttcc gcttcttcgg ttgaaaaatc
     9961 aaggctcagg gcggtgagga tgacttcaat ttttaagcgt ctatcttctg cagaatgaag
    10021 catactgaga agccagcgca tgagaggaat tcgcctgacg caagaggcaa aaatttcttt
    10081 acgggctaga atactcgcgt ctgcaaaagc ctgacccaaa gcggtcaatt caatatctcc
    10141 cttggcgatc gaagagaaac ccaataactc ggcagcctct gttagcttca gcaattgatc
    10201 ggatgtaatg cccagcttat cttctaacag gtagatatcg tgcttctgat tacctccttc
    10261 ggcgagattt tccaataacc cggctaaatc gcttatttca acctcaggaa gatgacgggt
    10321 gatacccact tcaccaggcg ctgtgcccag ctcttctttt tctgggcgtg tttgaccggc
    10381 taaaaccgcg taaacctggt ccacgtgatt ttgaaactcg actgatttac gattgcgcgg
    10441 atgggggagg tctattttat gctcggcgat aatacgccca ggttctttat ccatcaccac
    10501 aatacggtct gccaggaaaa cggcttcttc gatattgtgg ctgaccatca aaatcgcctt
    10561 ggttgggatt ttgccttcgg tccataattc cagcagttcc ccgcgcaggg attcagcact
    10621 taaaacatcc aaagccgaaa aaggttcgtc catacataac aactcaggtt caaccgccat
    10681 ggcacgggca aatccaactt tttggcgcat ccctcccgaa agctctctgg gataggcaga
    10741 ctcaaagcca tccaagccta cacggtccag caagtcttcg gcttttttgt ctctctcgtg
    10801 tttttcaaca ccttttgcct ttaacgcgag cgacacattc tccagcacag ttaaccaggg
    10861 gaacagtgca aaggtttgaa agataatcga tgtatggggg ttaactcctt ctaattgctg
    10921 accacgataa agcacctttc cctgggtggc tttttgtagg cctgcaataa tgcgcagcaa
    10981 cgtgcttttt ccacaaccag atggaccaag cagggcgaca aattcgccca ccgaaaggtt
    11041 caggttcaca ttctcaaccg cagtaaaacg acgcacgcca gccccgtaag actggaaaac
    11101 atgctcgagt tgcaacaagg cttctgtcat tttcactcca ttcggtattt ttcttcggct
    11161 aaacggtaca acctccgcca gatcactcgg ttgaccacaa cgacagtgat aatcatcgcc
    11221 agtgtacccg ccagcagtaa agggtaatca cctgaggcgg tcgcctgcgc tatcagcgaa
    11281 ccaataccgg ttacagtatg tgttttttcg ccaaactttt ggtattcagc cacaatgctg
    11341 gcgttccagg ctccgccgct ggcagtaatc gccccggtaa ccaggtaagg aaaaatagca
    11401 gggagcaaca acgttcgcca acgctgccag ccactgagac ccatgagttt tgaggtaaaa
    11461 attaaatctt gcgggatggc gctcgctccg gcaatgacat tgaatagcaa ataccattgg
    11521 gtacccatga gcattaacaa aaccgcggca gtgttcaatc ctccctgggc attcaggaaa
    11581 agcagtaaca gaacaggaaa aagggctgtt gcaggaacag aggcggtaac ctggacgata
    11641 ggctgaagca cggtggctaa agggcgattt gttccaatga ggacaccgac gggaatggtc
    11701 caggcaagcg caatcaataa tgccacaatt acccgcagaa aagtggctgt aactcccaac
    11761 ccaatgaccc cccatttttg aacagggacg gacaataaca tttctgttgc gtgataaagc
    11821 ccgtagctga tcaaggcaaa cacaatgatg gaaaaaacaa tcacatacca tttaagttgg
    11881 tttttaccat tttccaacct ggagacaggt ggggcatgtt tgattgttaa gcggtcaaac
    11941 cactccgtca gcggggcgaa aattcggttt gagataattc gggtaatgtt cgcgttgtgc
    12001 atcagatcca ggaaccacga ggttggcggg ttgtcgttct cgaccatttc aactttaaat
    12061 cgatctgacc aggcgatcag tggacgccaa atgagttggt ccaggagaac gacggtaaag
    12121 atcaacacgc ctgtccccag aaaaatggcc acatggttgc tttgattagc cgcttcctgt
    12181 aaataagcac ccaatccagg cagacgaaaa tcgcgtgagc ccacggtgaa gatttcggcc
    12241 gccattaaga agaaccaacc gcctgcccaa gacatcatgc tgttccatat caggctgatc
    12301 attccaaacg gtaattcgag cgccttgaaa ttgatccatt tattcaggcg aaagatagaa
    12361 ctggcttctt ttaattcttt gggaattgtg gtcagcgatt gataccaggc aaaggtaatg
    12421 ttccaagcct gactggtgaa gatcaatacg atggaagcaa tctcagccgc tgttccttct
    12481 ggcaggaagg aacttaaact caataagacg actggcagaa acgataaaat gggcacgctt
    12541 tgcagcacat ccaataaggg catcaggatt ttttcagcat tttgatgata agcggctact
    12601 cgcccgtaaa tgatggagaa caagaaagag aggagatagg cggctgccat tcgaccaact
    12661 gaaaaaagcg tatatattgg cagggctgat ggagataatg aaatatccgg tccagggacg
    12721 acttcaggcg cgttgaccgc taagcgcgcc cccacataca ataatgcgat caccagggaa
    12781 agggcgataa tatcgcccca ggtgaggggc ttgcggctat tttgcgatgt gccaaaaaca
    12841 cgggtgtttt gcgtcatctc ttcatcacct tcaggatatc atcgacctgg aggtgcagtt
    12901 ttgacgctac aaaaccaggt tgaaacccca aataatcata ctgaggtcat cgggatcatc
    12961 agggttcatt cgtttttcag tatgagcaca catttcctca ttcataatta aaattatacc
    13021 ctatcgctat gagaatggcg cttcacaact tgcttggctt gaaaatgatg ggcttaaacc
    13081 caacactgag cgcatgtgat ttaaggatgg acaacttaac aggcatgtaa actggagtgc
    13141 cgctaatctt cgttataatc atctgaggag caggaaaaca tgattacaga tacccgtcag
    13201 aactatcgtg tactgttacc cgggttgctg ttgggattgg ccgtttttgc actgctgata
    13261 tctctcagca gcatccacca ggttttcacc ttgtttgagt ctttcaagtg ggtgtatttt
    13321 gcggtcgcgg tagggttgaa catcatccat catgccctac gcttcctcaa cctttctttc
    13381 tcattgaata tatctggaat tcgcaggatt cccctaaaaa aacgcatggc tttctacctt
    13441 tcaagctcag cgctgaccgc cgcagaccca aaagcccagg aatcctatcg ctcggtttgg
    13501 ttgagtaaag cctgcggcat cccgcttgtc cggatcgatt caattttcct tatcgaccgc
    13561 ctttctgacg ggttgagtat tttggcgctg gccacgctgg gcatcctggc ttaccccgct
    13621 ttctggccat tcttcgcggc gatgctgttg ttgtttgtgt tatccatcct cttcttacag
    13681 atcaagccgg ctggagaagg tttgctggat gcgggggaaa agcttcctct gctgcgcctg
    13741 tttagccggc agttacgcca gtgtatggaa ggcaatgaac atcttttcag aacctttccg
    13801 atgacggtat cgcttttttt caacgtgctg gcgtggattg ccctggccgc cgcgctcttt
    13861 ttcgtcttgg ttggttttgg gcttgaaccc agcctcaccc ttgcggccat cgcctgcctg
    13921 gtactggcgt tttccatcat cattggtttc ttctccactg ttcctggtgg gttaggtgtg
    13981 gttgaactgg cgctggctgg cttgctcacc gttttactgg ggtttcaacc cgaacttgcc
    14041 gttactgcca ccatcctttt ccgtatgtct ac
//
LOCUS       DAOSPM010000047        13789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_4468_length_13789_cov_11.962356, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000047 DAOSPM010000000
VERSION     DAOSPM010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4468_length_13789_cov_11.962356"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PLW25_05560"
     CDS             complement(<1..1020)
                     /locus_tag="PLW25_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOS53991.1"
                     /translation="MSNFLQLIAEIAIILLAAKAAGYLSVRLKQPSVFGELLVGVLLG
                     PSLLDFTNLGFVTSTHLSETIHELGEIGVLLLMFLAGLELNIKDLTRNTRVALLAGML
                     GVVFPVGFGIVFGELLDIPFRQALFLGLTLGATSVSISAQVLIELQALRTRVGLALLG
                     AAVFDDILVILILSSVLAFLSGSGGFREIMWVFAKMVIFLVLAAAAGLWLLPRMVRLT
                     RSLKISQGLTTLALVVLLGYGLAAELMGGMAAITGTFLAGLMFARTPEKGTIETNMHP
                     IAYAFFVPIFFVSIGLNVNLRELNADSIWLFLGICGIAIAGKVIGSGLGALLAKFTSR
                     EALQIG"
     gene            1135..>1499
                     /locus_tag="PLW25_05565"
     CDS             1135..>1499
                     /locus_tag="PLW25_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOS53992.1"
                     /translation="MNLESEKKRWDEEVVKPVVEKFKERKDEFTTPSGIPIPRAAIPG
                     SADYMEQLGFPGEYPFTRGVQPTMYRSRFWTMRQYAGFASAEESNRRYRYLLEQGQTG
                     LSVAFDLPTQIGYDADDSMA"
     assembly_gap    1500..1599
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1600..2559
                     /locus_tag="PLW25_05570"
     CDS             <1600..2559
                     /locus_tag="PLW25_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOS53993.1"
                     /translation="SGYHIREAGSTAVQEVAFTLANAIAYVQAAIDTGQQVDQFASQL
                     SFFFAAHNDFLEEIAKFRAARRLWARIMRERFGAADPKSWMLRFHTQTGGSTLTAQQP
                     ENNIVRVAIQALAAVLGGTQSLHTNSKDEALWLPTEKSVRTALRTQQIIAYESGVADT
                     IDPMGGSYVIEHLTDEIERRVMEYIKKIDDLGGALAAIEKGFMQNEIQDSAYRFQKDV
                     ENKAQLVVGVNAFQSNDTSDLEQLRVDPAIEQQQRVRLAELRANRDQAKVNELLGHLE
                     STAKASENLMPLFITCVENEITLGEICGVLRTVWGEYQPPVWG"
     gene            2578..2985
                     /gene="mce"
                     /locus_tag="PLW25_05575"
     CDS             2578..2985
                     /gene="mce"
                     /locus_tag="PLW25_05575"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOS53994.1"
                     /translation="MAKIKKINHVAVVVGDVDEALKFWQDAMGIQLHHLEDVPSQKAQ
                     VAFLPVGESEVELVKPTSDDSGVAKFLAERGGGMHHLCFEVDDIAGMMAQLKEKGVRL
                     LSEEPQVLPGRKMVFIHPKSTGGVLVELYEVTK"
     gene            complement(3046..4383)
                     /locus_tag="PLW25_05580"
     CDS             complement(3046..4383)
                     /locus_tag="PLW25_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS53995.1"
                     /translation="MAAMKEKATESERLHKQEIHAWTMYDWANSSFATTMMAAVFPIY
                     FATLGAAGETGAIMTSRWGFTTAFAALLAAVISPLLGAMADFRGSKKKFLTVFMLLGV
                     IATALLFFPNQPGSWLIAAILFIFANIGFAGSLVYYDALLPHVARADEIDQVSSRGYM
                     MGYFGGGILLAVNLVMIMVLPEVFPQLGKGLMTRLSFVTVALWWFGFTLPLLFKVKEP
                     LRRIEEGEEDLHPIRASLIRLKKTFLDLKKYRDLSLGLLAFWIYANGIGTIITMATIY
                     GKELNFSDTTLIGTLLMVQILAAPFAMLFGWLAKKIGTKKAIYLSLVIYSLIAVAGYF
                     LYHEWQFWVLGAAVATVQGGSQALSRSMIGKMMPKSKSAEFYGFFSVFEKFASILGPF
                     IFGVVSHLMNESRLSIASLILFFGLGLFVLTKVNLERGVKVASDEEAKLITVE"
     gene            4725..>6199
                     /locus_tag="PLW25_05585"
     CDS             4725..>6199
                     /locus_tag="PLW25_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOS53996.1"
                     /translation="MTQNCIRVVGARAHNLKNITVEIPRDKLVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFLGQMEKPDVDYIEGLSPAVSIDQKATSHNPRSTVGT
                     VTEVYDYLRLLFARIGIPHCPVCGRVVAKQSAQEIVDQVEALPEGSRLLILAPVVRAR
                     KGTYQAAFEEIRKAGFVRVRVDGQVYNLDQDIVLDRYKSHNIDAVVDRIVITRSEDEE
                     EQRSFRSRLTDSIETGLKVGEGNLIVQEVSGDTPLDLFYSEHLACPEHNISIPEIEPR
                     TFSFNTPHGACPECQGLGSKLEIDPDLIIPDPERSMREGAIAALEWNGPRDQGGYYWQ
                     MIEAVAKRYSIDLDAPVGSIPQEKLDKILFGTGSESIPVVMEGRNERKTTMQLSFEGI
                     IPNLERRFRETHSEYIRNRISEFMSDRPCPTCKGERLRPEALAVTVLDHNIVEVTNWP
                     VNRTLEFIASLSTSGVLSNRDFTIAERIIKEITSRLGFLVNV"
     assembly_gap    6200..6299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6300..7457
                     /locus_tag="PLW25_05590"
     CDS             <6300..7457
                     /locus_tag="PLW25_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS53997.1"
                     /translation="PGAGEHGGEVVAQGTVDAIINHPHSLTGAYLSGRKKVPLPKKLH
                     PRNGKTLKIVGARANNLKNLEVEIPLGQFVCITGVSGSGKSTLMVDLLYNVLAHELNR
                     ARTQPGEYDRIEGMQYLDKIINIDQSPIGRSPRSNPATYTGMFDEIRSLFASLPESKM
                     RGYKAGRFSFNVHGGRCEACQGQGQLRIEMQFLPDIYVPCDVCHGTRFNRETLQVKYK
                     GLSIADVLDLTVEEGIKLFGNIPAIANKLHTLEKVGLGYIRIGQPGTTLSGGEAQRVK
                     LSKELSRRATGRTMYVLDEPSVGLHAADVHMLIDVLQHLVDSGNSVLIIEHNLDIIKV
                     ADYLIDLGPEGGDRGGELIATGTPREVCDVPTSYTGQVLKAYLQLHQQDPQ"
     gene            complement(7509..8822)
                     /locus_tag="PLW25_05595"
     CDS             complement(7509..8822)
                     /locus_tag="PLW25_05595"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOS53998.1"
                     /translation="MSKICVIGTGYVGLVTGTCFADLGNEVICLDIDHERIASLKAGQ
                     MPIYEPGLEQLVKQNVAAERLFFTTDYAIALKDAEYAFIAVGTPSGNDGEADLRYVRS
                     AAEAIADHVKNAILVINKSTVPVGTGDWVADVINRRRHGKALNFSVVSNPEFLREGSA
                     INDFMNPDRVVLGSEDQSAADRVAQLYQPLRCTIMTTDLRTAEMIKYASNAFLATRIS
                     FINEIANICEELGADVREVARGMGLDKRIGPQFLDAGLGWGGSCFPKDVKALEHMAVL
                     HGTQPQLLQAVMEINRNQRRRAVMKLRKSLKTLNEKTIGVLGIAFKPNTDDIRDAAAI
                     ELIHLLQNEGAQIKAYDPQAMENASHVLKNVVLCENPYQVAEGADALVLATEWNEFKQ
                     LDFKKLKSIMKQAVILDGRNLWDASALKDLGFTYMGIGRYHLPGK"
     gene            8928..9806
                     /gene="folD"
                     /locus_tag="PLW25_05600"
     CDS             8928..9806
                     /gene="folD"
                     /locus_tag="PLW25_05600"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOS53999.1"
                     /translation="MTATIIDGKAIGDELRREVAAGVQQRLDSGKSRPGLATVLVGEN
                     PASQVYVKSKQKACAEVGIESFGHELPRDASQAEVERLVTQLNADPAVNGILVQLPLP
                     SGLDEEKILSAISIEKDVDGFHPINIGRLAQKGRDPLFVPCTPAGVMYMLEKEFPSLE
                     GLNAVVLGRSNIVGMPVALLLVRANATVTICHSRTKNLPDVVRSADVLVAAVGKAEMV
                     RKDWIKPGAVIIDVGINRVEDATKPRGYRLVGDVCFDEAAEVARAITPVPGGVGPLTI
                     AMLLRNTLRAASLQDA"
     gene            9913..11295
                     /locus_tag="PLW25_05605"
     CDS             9913..11295
                     /locus_tag="PLW25_05605"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOS54000.1"
                     /translation="MSTLLVKNAHILVTMDDLRREIRGGGLFIRDGFIEQAGATDQLP
                     ATADEVLDLTDHILLPGLVNTHHHFYQTLTRAIPAAQNANLFHWLKTLYPIWAKMTPE
                     DIRLSTQTALAELALTGCTTASDHLYLFPNGSRLDDEIEAAADLGLRLHASRGSMSLG
                     ESQGGLPPDSVVDSEEKILADSERLIQKYHNPNPGSMVQIVLAPCSPFSVTGELMKQS
                     AELARRYGVHLHTHLAETQDEEAFCRQKFGYRPVGYMQSLGWVGEDVWFAHSVHVNDE
                     EIDVYARHGCGVAHCPSSNMRLASGIAPIARLVDAGVKTGLGVDGSASNDGSHLLAEA
                     RMAMLLARLNAGLQGASLSTTSAEIFSVRQALELATRGGAAVLGRKDIGSLEAGKCAD
                     FIAINLNRLDYAGALHDAVAAVLLCQPRGVDFNYVHGKGVVKKGVLSGLNLEAHIHLH
                     NRAATRLFEG"
     gene            11307..13103
                     /gene="ade"
                     /locus_tag="PLW25_05610"
     CDS             11307..13103
                     /gene="ade"
                     /locus_tag="PLW25_05610"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOS54001.1"
                     /translation="MGMDSFWLQNARELVDCAMGRLPAHLIIKNGQWVCVQTGEIIPK
                     TDVAMRAKRIAYVGEDASHTIGPKTVVIDADGQYLVPGLLDGHVHIESGMVTVTEFVR
                     AVLPHGTTGLFIDPHEIANVFGLRGVRLMVDDAALQPVHVFVQMPSCVPSAPGFETPG
                     AVLGPKDVTEAMTWPGIIGLGEMMDFPGVYEGDDKVHAEMSATRAAGKVIGGHYASPD
                     LGRRFHGYVAGGPQDDHEGTRLEDAVARARQGMKVMMRYGSAWHDVAAQVKAVTELGL
                     SSRQFLLCADDILSETLVNEGHMDRIIRHAIAEGLDPMTAIQMATINTAEHFGVSREI
                     GLIAPGRFGDVVLVKDLRDFQVETVVARGKLAYQKKTILVALPAREYPEWAVRSVHFP
                     RPLSAASFRLGAGGSQEVLANVIGIIENQAPTRHLLFKMTVKDGEIYPDLARDIAKLA
                     LVERHRPTGQVRVGLVHGFGFKIPCAVATTVAHDSHQMLVAGTDETQMAIAANHLESI
                     GGGQVIVTNNTVIGEIRLPIAGLMSTGSAQQVADETASLLEGFKQCGCTLNNPNMQLS
                     LLGLPVIPELRLTDLGLVDVNRFDFIPVLQHE"
     gene            13251..>13789
                     /locus_tag="PLW25_05615"
     CDS             13251..>13789
                     /locus_tag="PLW25_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOS54002.1"
                     /translation="MAKSLGVSRATLREGMRTFEARGLIRRRQGVGTFVVSQLKVIET
                     GLEVLESIERLANRIGLAVRMGELVISETMANPEISERLQVEEGTRLTNIARVIYADD
                     RPVAYLVDILPPNILAESELQSGFNGSVVDFLIQRGNPGLSKSFTDIQAVAAESSIAR
                     LLEIQRGDVLLLFTARLYS"
BASE COUNT         3239 a         3487 c         3857 g         3006 t
ORIGIN      
        1 accgatttgc agcgcttcgc gcgaagtgaa tttcgccagc agtgccccca gtccggagcc
       61 gatgacctta cccgcgatgg cgataccgca aatgcccagg aataaccaga tggaatcagc
      121 gttcagttca cgcaggttca cattcaaacc gatgctgacg aaaaagatgg gcacaaagaa
      181 agcgtaagcg atgggatgca tgttcgtttc gatggtgcct ttttcaggtg ttcgggcgaa
      241 catcagcccc gcgaggaatg tgccggtgat tgcggccatg ccgcccatca gctctgctgc
      301 cagcccataa cccaacagca ccaccagggc cagggtggtg agtccctggc tgattttaag
      361 agatcgtgta aggcgcacca tgcgcggcaa cagccacaac ccagccgcgg cagccagcac
      421 caaaaagatg accatcttcg caaaaaccca cattatttcc ctgaatccgc cgctgccaga
      481 caggaaggct aaaacagaag agaggatcag gatgaccagg atgtcgtcaa aaaccgcggc
      541 gcctaaaagg gcgagcccca cccgcgtgcg cagcgcctgc agttcgatca gcacctgggc
      601 ggaaatggaa acggatgtgg cgccaagcgt gagccctaag aacagcgcct gacggaaggg
      661 aatatccaat aattcgccga aaacgatgcc aaaccccacc ggaaagacca ctccaagcat
      721 gcctgccagc aatgccacgc gtgtgttgcg ggtgaggtct ttgatgttca gctccagccc
      781 tgccaggaac atcagcagca gcacgccgat ctcgcccagt tcgtgaatgg tttcgctcag
      841 gtgggtagag gtgacaaagc ccaggttggt gaaatccaac aacgaaggcc caagcagcac
      901 gcccacgagc agttcgccaa ataccgaagg ttgctttagg cgtacgctta aataacccgc
      961 ggctttggcg gcgagcagga tgatggcgat ctcggcgatg agttgaagga aattcgacat
     1021 ggattgacgc gctaatggat ttttatttca gtttatcata atttctcaca catccatttt
     1081 ggattcgtgt atactcaatt cagatggctc atcaatattt tcagaggcga aaacatgaac
     1141 cttgaaagcg aaaaaaaacg ttgggatgaa gaagtggtaa agcctgtggt agaaaaattt
     1201 aaagaaagga aagatgaatt caccacgcca tccggaatac ccatcccgcg cgcagccata
     1261 cccggtagcg ctgattacat ggaacagctg ggtttcccgg gtgaataccc tttcacccgc
     1321 ggcgtccagc ccaccatgta ccgcagccgc ttctggacca tgcggcagta cgccgggttt
     1381 gcctcggcgg aagaatccaa caggcgctac cgttacctgc tggagcaggg gcagaccggc
     1441 ctttccgtgg ctttcgatct gcccacgcag attggttacg acgcggatga ttcgatggcn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ccggctatca tatccgcgag
     1621 gctggctcaa ccgccgtgca agaggtggct ttcaccctgg cgaacgccat cgcatacgtg
     1681 caggcagcca tagacacggg ccagcaagtg gaccagttcg cttcgcagct ttcctttttc
     1741 tttgccgcgc acaatgactt tctggaggag attgccaaat tcagggcggc gaggcgctta
     1801 tgggcgcgta tcatgcgcga acgtttcggc gcggcagatc ctaaatcctg gatgctgcgc
     1861 tttcacacgc agacaggcgg ttcaacgctc accgcgcagc aacctgaaaa taacattgtg
     1921 cgtgtggcaa tccaggcgct ggcggcagta ttgggcggca cgcagtcgct gcacaccaat
     1981 tccaaagatg aagcgctttg gctgcccact gaaaaatctg tgcgcaccgc gctgcgcacc
     2041 caacagatca tcgcttacga atccggcgtg gccgatacca ttgacccgat gggcggctcg
     2101 tatgtcatcg agcacctcac agatgagatt gagcggcgcg tgatggagta cataaaaaag
     2161 atcgatgacc tgggcggcgc gctggcggcg atcgaaaaag ggttcatgca aaatgagatt
     2221 caggattctg cctatcgctt ccagaaagat gtggagaaca aagcgcagct ggtggtgggc
     2281 gtcaatgctt tccagtccaa cgacacttcc gatctggagc aactgcgggt tgacccggcg
     2341 attgagcaac agcaacgcgt tcggctggct gaattgcgcg ccaatcgcga ccaggcgaaa
     2401 gtgaacgagt tgcttgggca tcttgaatca acagcgaaag cgagtgagaa tttaatgccg
     2461 ctcttcatca cttgtgtgga gaacgagatc accctgggtg agatttgcgg agtgctgcgc
     2521 acggtttggg gcgagtatca accccctgtt tggggataac aatcacagga gagcgaaatg
     2581 gcgaaaatca aaaaaattaa tcacgttgcg gttgtagtgg gagatgtgga cgaggcattg
     2641 aagttctggc aggatgccat gggcattcag cttcaccacc tggaagatgt gcccagccag
     2701 aaagcccagg tggcgttctt gcctgtcggc gagagcgaag tggagctggt gaaacccacc
     2761 agcgatgaca gcggcgttgc caaattcctg gcggagcgcg gcggcggtat gcatcacctg
     2821 tgtttcgaag tggacgatat tgccggtatg atggcgcagt taaaagaaaa aggggtcagg
     2881 ctgttaagtg aagaaccgca ggtgctgccc gggcggaaaa tggtgttcat tcacccaaag
     2941 agcaccggcg gcgtgctggt ggaactatac gaagtgacga agtagacgag cacaatcaaa
     3001 gaccggaagg tggtcaaatt ccttccggtc tttttgttca aacatttatt cgactgttat
     3061 taatttggct tcttcatctg acgccacttt cacccctcgc tccaggttca ccttggtcag
     3121 cacgaacaac ccgagaccga agaacaggat caaagaagcg atacttagcc tggattcatt
     3181 catcaggtgt gatacgacgc cgaaaatgaa cggtcccaag atagaagcga atttttcaaa
     3241 cacgctgaaa aagccataga attcagcaga tttactcttg ggcatcatct taccgatcat
     3301 tgacctggaa agcgcctgac tgccgccctg cacggtggcg actgcggctc cgagcaccca
     3361 gaattgccat tcatggtaga ggaaataccc ggcgaccgcg atgagagaat aaatgaccag
     3421 gctgaggtag atggctttct tggtgccgat cttctttgcc aaccagccaa acagcatggc
     3481 gaatggcgct gccaggatct gcaccatcag cagtgtgccg atcaaggtgg tatcgctgaa
     3541 gttcaattcc tttccgtata tggtggccat ggtgatgatg gtgccaatac cattggcgta
     3601 gatccagaaa gccagcaatc ccagtgaaag gtcgcggtat ttttttagat ccaaaaaagt
     3661 ttttttcagg cgtattaagc tggcccgaat ggggtgcaaa tcctcttccc cttcttcgat
     3721 acggcgtaat ggttctttta ctttgaagag cagaggtaat gtaaaaccga accaccacaa
     3781 agccacggtc acaaatgaca ggcgggtcat caacccttta cccagttgcg gaaaaacctc
     3841 cggcaggacc atgatcatga ccaggttgac agccagtaaa attcccccgc cgaagtagcc
     3901 catcatgtaa ccgcgagaag aaacctggtc gatttcatcc gcgcgggcca cgtgtggcag
     3961 cagcgcgtca tagtagacca ggctgccggc aaaaccgatg ttggcaaaga tgaacaaaat
     4021 ggctgcgatc agccaggaac caggctgatt agggaagaaa agcagggcag tggcgataac
     4081 ccccagcagc atgaagacgg ttaagaactt tttcttggag ccgcgaaaat ccgccatcgc
     4141 gccgagcagc ggactaatta cagcggccaa cagcgcagca aacgcggtgg taaagcccca
     4201 gcgcgaggtc atgatggcac cggtttcacc agccgcaccc agggtggcga agtagatcgg
     4261 aaagaccgct gccatcatgg tggtggcaaa agaggagttc gcccagtcat acatcgtcca
     4321 ggcgtggatt tcttgcttgt gaagccgttc actttcggtt gctttttctt tcattgctgc
     4381 catggcgcct ctctcctgtc aatactgatt gattgtttta ttatacagta gaggggtcgc
     4441 agcgtggttc aactggtcag gtgaggttct gcctgggttt atgacgggca gccgcgtaca
     4501 ccaggctgta cctttctttt cagttctggc agcgctgtct ttcgctgaag atgagcagcg
     4561 gcgggtgagc caaagaggct cttgaacaag aagcaggcag acggtctgct gtgattcacg
     4621 agggtggaaa ttaaagcgcg gaaggggcag acagcaaggg tataattaaa ggttggtttg
     4681 tgataaaata gtgtaattag aatatatgtt cgattggaga aacaatgacg caaaattgta
     4741 tacgagtggt tggcgcgcgg gcgcataacc ttaaaaatat cacagtggaa ataccacggg
     4801 ataaattggt ggtgattacc ggtctctcgg gttctggcaa aagttcactg gcatttgata
     4861 ccatttttgc tgaagggcaa cgacgctacg tggaatcgct ttcagcttat gcccggcaat
     4921 tcctcgggca aatggaaaag ccggacgtgg attatattga aggcctatcg ccggcggttt
     4981 ccatcgatca gaaggctacc tcgcacaatc ctcgctccac agtcgggacg gtgaccgagg
     5041 tgtacgatta cctgcgcctg ctcttcgcac gtatcggcat tccacattgc ccggtttgcg
     5101 ggcgagtggt agcgaagcaa tctgcccagg aaatagttga tcaggtggaa gcgctgcccg
     5161 agggctccag gctgctcatc ctggcgccgg tggttcgcgc ccgcaaaggc acttaccagg
     5221 cagcttttga agagatccgt aaggctggtt ttgtgcgcgt acgcgtggat gggcaggtgt
     5281 acaaccttga tcaggacatc gtgcttgacc gttacaaaag tcacaatatt gacgcggtgg
     5341 ttgaccgcat tgtgatcacc cgctctgagg atgaagaaga gcagcgttct tttcgttcca
     5401 ggctgactga ttcaatcgaa accgggctga aggtgggcga gggtaacctc attgtgcaag
     5461 aagtgagcgg tgataccccg cttgacctgt tctattcaga gcaccttgcc tgcccggaac
     5521 ataatatcag cattcctgaa attgagccgc gcacgttttc ctttaacaca cctcatggcg
     5581 cctgccccga atgccagggg ctggggagca agctggaaat cgatccagac ctgatcatcc
     5641 ctgaccccga gcgatcgatg agggaaggtg ccatcgctgc gttggaatgg aacggtccgc
     5701 gagaccaggg ggggtactac tggcagatga ttgaagcggt agccaaaagg tattctattg
     5761 accttgacgc gcctgtgggt tcgattcctc aggaaaagtt ggacaagatc ctgtttggca
     5821 cgggcagtga atccataccg gtggtgatgg aaggacgcaa tgaacgcaaa accaccatgc
     5881 agttgagctt tgaaggcatt attcccaacc ttgaacggcg cttcagggaa acacactctg
     5941 aatatatccg taaccgtatc tccgagttta tgagtgaccg cccatgccca acctgtaaag
     6001 gcgagcgcct tcgccctgaa gcgctggctg tgaccgtgct tgaccacaac atcgtggagg
     6061 tcaccaactg gccggtcaat cgcacacttg aattcattgc gtcactttca acctccgggg
     6121 tgctctccaa ccgggatttc accattgctg aacggatcat caaagaaatc acttctcgcc
     6181 tgggctttct ggtaaatgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     6301 cgggggcagg ggaacatggc ggcgaggtgg tggcgcaggg aacagtggat gcgattatca
     6361 accatccgca cagcctcacc ggggcctatc tttcaggcag aaaaaaggta ccgctgccca
     6421 agaaattgca cccgcgcaat ggcaaaacac ttaagatcgt tggcgccagg gcaaacaatc
     6481 tcaagaacct cgaggtggaa atacccctgg ggcaattcgt ttgcattacc ggcgtttccg
     6541 gttcgggcaa gtcgaccctg atggtggacc tgttgtataa cgtattggcg catgaattga
     6601 accgcgcgcg cacccaaccc ggagagtatg accgcattga aggcatgcaa tatctggata
     6661 agatcatcaa tattgaccaa tcacccatcg ggcgcagtcc tcgctcaaac cccgcgacct
     6721 acaccggcat gtttgatgag attcgcagcc tgtttgccag tttacctgag agtaaaatgc
     6781 gcggctacaa agccggacgc ttctcattta atgtgcatgg cgggcgctgc gaagcctgcc
     6841 aggggcaggg tcaattgcgc atagaaatgc agtttttacc ggatatctac gtgccctgtg
     6901 atgtttgcca tgggacgcgc ttcaatcggg aaaccctgca ggtgaagtat aaggggcttt
     6961 cgattgcgga tgtgcttgat ctgaccgttg aagagggtat caagttattt gggaatatcc
     7021 ccgcgattgc caataaactc catacattgg agaaggttgg cctggggtat atccgcattg
     7081 ggcagccggg aaccacgctc tccggcgggg aggcgcagcg cgtgaaactc tccaaggaac
     7141 tttcacgccg ggcaaccgga cgcaccatgt acgtccttga tgaaccctca gtggggctgc
     7201 atgcagcaga tgtgcatatg ctcattgatg tgctgcagca cctggtggat agcggtaatt
     7261 cggtgctcat cattgaacac aacctggata tcatcaaagt cgcagattat ctcatcgatc
     7321 tcggacccga aggtggcgat cggggtggtg aacttatcgc caccggcacg ccacgagaag
     7381 tgtgcgatgt gcccacttct tacaccgggc aggttctaaa agcctatttg cagcttcacc
     7441 agcaagatcc ccagtgacag tgtaataaac aagaggtggc tcaagtgagc cacctcttgt
     7501 ttgacttttt atttgccggg cagatgatag cgcccaatcc ccatgtaggt gaatcccagg
     7561 tctttcagcg cgctggcgtc ccaaaggttg cgcccatcca ggatcactgc ctgtttcata
     7621 atggatttga gtttcttaaa atccagttgt ttaaattcgt tccactcggt tgccagcacc
     7681 agggcatcag cgccttctgc cacctggtaa gggttttcac aaagcaccac atttttaagc
     7741 acatgactgg cattttccat tgcctgcgga tcgtaagcct tgatttgagc gccttcattt
     7801 tgcaacaggt gaatgagctc gatcgccgcc gcgtcgcgga tgtcgtccgt gttgggttta
     7861 aaggcgatgc cgagcacgcc aatcgttttc tcgttgagcg ttttaagaga cttgcgcaat
     7921 ttcatcaccg cgcggcgccg ctggttacgg ttgatttcca tgaccgcctg cagcagttgc
     7981 ggttgagtgc catgaagcac ggccatgtgt tccagggctt tcacgtcttt ggggaagcag
     8041 gagccgcccc agcccagccc ggcatcgaga aactggggac cgatgcgctt atccaaaccc
     8101 attccacggg caacttcgcg cacatccgcg cccaattctt cgcagatatt ggcgatttca
     8161 ttgataaatg aaatgcgcgt tgcaagaaac gcgttcgaag cgtatttgat catttccgcg
     8221 gttcgcaggt cagtggtcat gatggtacag cgcaatggct ggtagagctg ggccaccctg
     8281 tctgccgccg actggtcttc cgagcccaac accacccggt cgggattcat aaagtcatta
     8341 atggcagagc cttcgcgtaa aaactcaggg ttcgagacca cgctgaaatt taatgcttta
     8401 ccgtgtctgc gccgattgat cacgtctgcc acccaatcgc cggtaccaac cggtaccgtg
     8461 gatttattga tgaccaggat ggcgttcttt acatggtcgg caatggcttc ggctgccgag
     8521 cgcacgtatc gcaaatctgc ttcgccgtca tttccagagg gcgttcccac ggcgataaag
     8581 gcgtattcag cgtccttcag cgctatagcg taatctgtgg tgaagaacag gcgttcagcg
     8641 gcgacgtttt gtttgacaag ctgctcaaga ccaggttcat aaataggcat ttgcccggcc
     8701 ttaaggctgg cgatgcgttc atgatcgatg tccaggcaga tgacctcgtt tccaaggtca
     8761 gcaaaacagg ttccagttac caagccaaca taacccgtcc cgatgacgca aattttactc
     8821 atgtgtctct cccattgttg cgttgtacga ccagctccat tttaccatca cgccctcgga
     8881 aacttaaaat aggtataatt aattcatcaa acataaggag cgaaaaaatg acagccacga
     8941 tcatagatgg aaaagcgatc ggcgatgaat tacgaagaga ggtggcagca ggggtgcagc
     9001 agcgcctcga ttcaggcaaa tcaaggcctg gcctggcaac tgtgttggta ggcgaaaacc
     9061 ccgcatccca ggtatatgta aaatccaagc agaaggcttg cgctgaagtg ggtatcgagt
     9121 cattcgggca cgagttgccg cgggatgcca gccaggcgga agtggaaaga ttggtcactc
     9181 agctcaacgc agacccggcg gtcaatggca ttctggtaca gctgcctctg ccgtcagggc
     9241 ttgatgaaga aaagatactc agcgccattt ccatcgaaaa ggatgtggat ggtttccacc
     9301 ctatcaatat tggccggctg gcgcaaaaag gacgcgatcc gttattcgtt ccctgcacac
     9361 ctgcaggggt gatgtacatg ctggaaaagg aattcccctc gctggaaggg cttaacgcgg
     9421 ttgtgctggg caggtccaat atcgtcggta tgccggttgc cctcttgctg gtgagagcaa
     9481 acgccactgt cactatttgc cactcgcgca cgaagaacct tccagatgtc gtgcgttcgg
     9541 cggatgtttt ggtggcggcg gttggcaagg cggagatggt aagaaaagac tggataaaac
     9601 ccggagcggt aatcattgat gtgggcatca accgagtgga agacgccacc aagccccgcg
     9661 gctaccgtct ggtgggagat gtgtgttttg atgaagcagc ggaggtcgcc agggcgatca
     9721 cgcctgtccc cggcggggta ggacctctaa ccattgccat gctgctcagg aacaccttaa
     9781 gagcggcgag ccttcaggac gcctgagctc tctcagttgt ctgacaattg acttaattat
     9841 taaatcgaat tacactcaaa ttcgttcgat gggttgcacc tcgtttcttt ggtcatgttg
     9901 aggaggtgac ttatgagcac acttctggtc aaaaatgcgc atatcctggt aacaatggat
     9961 gacctccgca gggagatccg aggaggtggt ctctttattc gcgacggttt catcgaacag
    10021 gcaggtgcca ccgaccagct ccccgcgact gctgatgaag tacttgatct cacggatcac
    10081 attctactgc caggattggt caatacccat catcatttct accagacgct cacacgggca
    10141 attcccgccg cccaaaacgc caacctgttt cattggttga agacgctgta cccgatatgg
    10201 gcgaaaatga ccccggaaga tatccgcctt tccacgcaaa cagccctggc tgagctggcc
    10261 ctgacaggct gcaccaccgc ttctgatcat ctgtacctct ttccgaacgg gtccaggctg
    10321 gatgatgaga ttgaagcagc tgctgaccta ggtttgcgcc tgcacgcatc gcgaggatcg
    10381 atgagtctcg gcgaaagcca gggcgggtta ccgcctgata gcgtggtgga ttcagaagag
    10441 aagatactgg cggatagcga gcgactgatt cagaaatacc ataatcccaa tcctggttcc
    10501 atggtgcaga tcgtgctcgc cccctgttcc ccgtttagcg tgaccggtga attaatgaaa
    10561 cagagcgcgg aacttgccag gcgttatggc gttcatctcc atacacacct ggctgaaacc
    10621 caggatgagg aagcgttttg ccggcaaaaa tttggttatc gaccagtcgg atacatgcaa
    10681 tccctgggtt gggtgggcga ggatgtgtgg ttcgcgcatt ctgttcacgt caacgacgag
    10741 gaaattgacg tttatgcccg gcacggttgc ggggtggcgc actgcccttc ttctaatatg
    10801 cgcctggctt ccggcatcgc tccgattgcc cggctggtag atgccggggt aaaaacgggc
    10861 ctgggggtgg atggctcagc cagcaacgac ggctcacatt tgcttgccga agcccgcatg
    10921 gccatgctgc tggcgcgttt gaacgccggt ctgcaagggg cctccttatc aacgaccagc
    10981 gcggaaattt tctctgtacg tcaggcgctg gagctggcta ccaggggcgg cgccgcggtg
    11041 ttaggaagga aggatattgg ttctctcgaa gccggcaaat gcgctgattt tatcgccatc
    11101 aaccttaacc ggctggatta cgccggcgcg ctgcacgatg ccgtggctgc tgtactgctt
    11161 tgtcagccca ggggcgtgga cttcaattat gtgcatggca aaggcgtggt aaaaaaaggc
    11221 gtgctatccg ggctgaattt agaagcacac attcacctgc ataaccgcgc cgccaccagg
    11281 ttgttcgaag gataaagggg tttcgcatgg gcatggattc tttctggctg cagaacgcac
    11341 gagaactcgt cgattgcgcg atgggtcgtc ttcccgcgca cttgataatc aaaaatggtc
    11401 aatgggtatg cgtgcaaacg ggcgagatta tccccaaaac ggatgtggcc atgcgcgcca
    11461 aacgcattgc ctatgtgggc gaagacgcca gccataccat tgggccaaaa accgtggtca
    11521 ttgacgcgga tggtcaatac ctggtgccgg gcttgctgga cggtcacgtt catattgagt
    11581 ccggtatggt caccgtcacc gagtttgtgc gcgcggtgct ccctcacggc acgactgggc
    11641 tattcattga tccgcacgaa atcgccaatg tttttggttt gcgtggtgtg cgcctgatgg
    11701 tggatgatgc ggcgttgcag cctgtgcatg tttttgtgca aatgccttcc tgtgtcccgt
    11761 cagcccccgg ttttgaaacc cccggcgctg tgctgggacc taaggatgtg accgaagcca
    11821 tgacctggcc ggggatcata gggctggggg agatgatgga tttcccgggt gtgtatgaag
    11881 gggatgacaa ggtccacgct gaaatgagtg ccacacgggc ggcgggcaaa gtaattggcg
    11941 gacattacgc ctcgcctgac ctgggaaggc gctttcatgg gtatgttgcc ggaggccctc
    12001 aggatgacca cgaggggacc aggttggaag atgcggtcgc tcgcgcccgc cagggtatga
    12061 aggtgatgat gcggtatggg tcagcctggc acgatgtggc cgcgcaggtt aaagcggtta
    12121 cggagttggg actttccagc cggcaattcc ttttatgcgc agacgatatc ctatcagaga
    12181 cactggttaa tgaaggtcat atggatagga ttatccgcca tgccattgct gaaggtttgg
    12241 acccgatgac cgcaatacaa atggcaacga taaataccgc cgaacatttc ggcgtttcgc
    12301 gtgaaatcgg gctcattgcc ccgggcaggt ttggcgatgt ggtgttggtg aaagacctgc
    12361 gcgattttca ggtcgaaaca gtggttgcca gggggaaact ggcctatcag aaaaagacca
    12421 tcctggtggc tctccctgcg cgcgagtatc ccgaatgggc ggtcaggtcg gtgcattttc
    12481 ccaggccatt gagcgccgcg agtttcaggc ttggcgcggg tggcagccag gaagttttag
    12541 cgaatgtgat tgggatcatt gaaaaccagg cccccactcg ccacctgtta ttcaaaatga
    12601 cggttaagga cggggaaatt tacccggatc tcgccagaga tatcgccaag ctggcgctgg
    12661 tggaacgcca ccggccgacc gggcaggtgc gggtagggct ggtgcacggc ttcgggttca
    12721 agattccctg cgctgtagcc actactgtag cacatgacag ccatcagatg ctcgtggcag
    12781 gtacagatga aacgcaaatg gcgattgcgg ccaaccacct ggagtcgata ggcggaggtc
    12841 aggtgattgt cacaaataac accgtgattg gtgaaattcg actgccaatc gcagggttaa
    12901 tgtctactgg cagcgcacag caggtggctg atgaaacggc gtcgttactt gagggtttta
    12961 aacaatgtgg ctgtaccttg aacaacccca acatgcagct cagtttgctg gggctgccgg
    13021 tcatcccgga actgcgcctg acagacctgg ggttggtgga tgttaaccgg ttcgatttta
    13081 tcccggtatt gcagcacgag taaaaaccca aaacaagcaa ggaacgattg acgatgagtg
    13141 gcagtccaac ttcaaagaca aagcggcctc ctttttctcg cttgcagaac gagctgtcgc
    13201 gattaattca aagaaccccg ccaggcgaaa aattaccctc tgaacccgtt ttggcaaaga
    13261 gccttggtgt ttccagggca acgttgcgcg aaggcatgcg cacctttgag gccaggggct
    13321 tgatccgccg cagacagggc gttggaactt ttgttgtctc tcagctgaag gtcatcgaaa
    13381 ccggcctgga ggtgctggag agcatcgaga gactggccaa ccgcataggg ctggcggtaa
    13441 ggatgggaga actggtcata tccgaaacga tggcaaatcc tgaaatctca gagagactgc
    13501 aggtcgaaga aggcaccaga ctgactaata tcgcacgggt gatctacgcg gatgaccgtc
    13561 cagtggctta cctggtagat attctgcccc cgaatatttt ggccgaatca gagctgcaat
    13621 ccggtttcaa cggatcagtg gttgacttcc tcattcaacg gggtaacccg ggtttatcta
    13681 aatcttttac cgatatccag gctgttgcgg ctgaatccag tatagccagg ttgctggaga
    13741 tacagcgtgg cgacgtgttg ttgttattta ccgcgcgctt gtatagtga
//
LOCUS       DAOSPM010000048        13535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_4575_length_13535_cov_14.641320, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000048 DAOSPM010000000
VERSION     DAOSPM010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13535
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4575_length_13535_cov_14.641320"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PLW25_05620"
     CDS             complement(<1..641)
                     /locus_tag="PLW25_05620"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54003.1"
                     /translation="MRNRYMVMIDMALTIIAVLGAYALRLELGALFFFYLPSAYWMIA
                     VSLLIKIPIYYLFSLYRRLWGYASTRELRLIFLAVTSASVLVSAVMILLLTFKVFNGF
                     PRSVLIIDWLLSIFLVGGTRFAIRLIADNVSTAANSMKKRPAKRALIIGAGDAGALVV
                     RELQKNPDLNLNPVGFLDDNPAKLKQQIHGIPVVGSLADIGKIIEHRKIEEVI"
     gene            complement(659..2080)
                     /locus_tag="PLW25_05625"
     CDS             complement(659..2080)
                     /locus_tag="PLW25_05625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOS54004.1"
                     /translation="MTVEKPQIKKRWRIRNGERQAILFLGDMLIAYLAVFVGLYFWGQ
                     KDWLDFSWAFLGQRAPFWFYLLPFLWVIFLIEVYDIRKANHRKDVIRGVATAAGISFI
                     IYLVIFFISDPNSLPRRGVFIFIIAATILTFAWRSLYIRIFTAPVFMRRALMIGAGKA
                     GSTLASMLREIHPGPFILVGYIDDDPEKQGEEISGYPVLGTAERMTAVIGEQDITDLI
                     FAISGELKPELFRAILNAAESDIEVTSLPKLYEEVFGRVPIFLLQTDWILRSFIDQTH
                     TSGVYESVKRLIDIAGSIVGLLLLVLLYPLIALSIIIDSGFPILYSQMRVGKNGHPYK
                     IFKFRTMYQDAEKDGVARTASANDARITRFGKILRRSHVDELPQCWNILRGENSIIGP
                     RAEQIELVNKFQQELPFYRARLFVRPGLTGWAQINQRYATTVEDTAVKLEYDLYYIKN
                     RNLLLDLTIILRTVGMVLGFKGL"
     gene            complement(2070..3086)
                     /locus_tag="PLW25_05630"
     CDS             complement(2070..3086)
                     /locus_tag="PLW25_05630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS54005.1"
                     /translation="MEPVKVSVIIPCYNEEATIEELLSALLAQTYPIDLMEVIIADGM
                     STDRTREKITAFKAGHPQLTIRVVDNHQHTIPAALNQAIQASTGAVLSRMDAHAIPAP
                     DYIEKCLRALNAGLGENVGGVINIRAGSDTWVGRAIAVATAHPLGVGDAHYRTATKAG
                     VTDTVAFGTYRRDVVKSIGGYDESLRINEDYEFNTRVRRSGGKIWVDPAIRVDYYSRP
                     DLRSLARQYFSYGYWKYKMLRRHPDSLRWRQALPPVFVSGILMLSLLSTFWIPARILL
                     AGVASLYLAILVAGTIQPAIQQKRSSLIPGVPLAIMTMHFSWGSGFLWSMLKSLVKGS
                     EHDR"
     gene            complement(3117..3527)
                     /locus_tag="PLW25_05635"
     CDS             complement(3117..3527)
                     /locus_tag="PLW25_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOS54006.1"
                     /translation="MACTDKKVYQSSEAPAAIGPYSVAAGGGPFVFSAGQIGIDPETG
                     NLVPGGIEEQTHQVFSNLSAVLRSASSCLEHVVKTTVFLRDMKDFPAMNKVYGGYFPS
                     NPPARTTIAVAGLPKDALIEIEVVALLCKDGDCA"
     assembly_gap    3589..3688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3712..4785
                     /gene="prfA"
                     /locus_tag="PLW25_05640"
     CDS             3712..4785
                     /gene="prfA"
                     /locus_tag="PLW25_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOS54007.1"
                     /translation="MLEKITAIENRYNELTRLMDENIQDYQKVVELAKERSELEPVIQ
                     LSARYRSLLNQLEQAKELESSGDEEMRALANLEIEALPPQIEELEKKLKRLLIPRDPR
                     DDRNVFIEIRAGAGGDEAGLFAAELMRMYMHYAEARGWSVEMISSNETGIGGFKEVIF
                     MVKGKGAYSRFKFESGVHRVQRIPATESSGRIHTSTVTVAVMAEVEEVDVQIPESDIT
                     IDVFRSSGAGGQNVQKNATAVRITHIPTGIVVACQDERSQLQNKLRAMGILRARLFEI
                     EEEKRRQERDENRRSQVGTGDRSEKIRTYNYPQSRVTDHRINLSSYNLPAVMTGEIDL
                     FIDELALRDETDRLSSSGDYDES"
     gene            4772..5617
                     /gene="prmC"
                     /locus_tag="PLW25_05645"
     CDS             4772..5617
                     /gene="prmC"
                     /locus_tag="PLW25_05645"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOS54008.1"
                     /translation="MTRVNLGRWIAQKRAQLQGKTDFPSIEINAIASHLLQQPTAWVI
                     SHPETVLTDAQYQLMEDAVNRLLLGEPLAYITGHKSFFGLDFIVDNRVLIPRPETEIL
                     VEHALAWLKAYPGKRKVIDIGTGSGAISITLVKNLAGLQVTAVDISAAALAVATANVH
                     YHKVANQIRLIESDLFEQVDQKFHLVLANLPYIPSIDLNSLSVVKYEPREALDGGSDG
                     TELITRLIDTMPHHLLPGGCAILEIQYNQDNLVSSIAARRFPDASISFIKDLASINRF
                     LKIQL"
     gene            5631..6254
                     /locus_tag="PLW25_05650"
     CDS             5631..6254
                     /locus_tag="PLW25_05650"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOS54009.1"
                     /translation="MQTITLPADNPASIETALDLLTEGEIVAFPTDTVYGLGANAFYS
                     PGIIKLFEAKGRDANKAIAVLIGDNGHLDLLTDFLSANARKLIKHFWPGGLTIVVPKK
                     KDLPELLSAGSSIGIRMPNHNIALELLNKFGPLATTSANLSGKNNPHNAGDVLNQLNG
                     RVPLILDGGRCPGGVPSTVVDCSTDEVRILRPGAISHEAIDAVISRL"
     gene            complement(6279..8015)
                     /gene="cydC"
                     /locus_tag="PLW25_05655"
     CDS             complement(6279..8015)
                     /gene="cydC"
                     /locus_tag="PLW25_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HOS54010.1"
                     /translation="MKGLWRVCIILKEFSGQVFLSLILGVAAVAAGIGLLGTSAFLIA
                     SAAFQPSIAELQVAIVGVRFFGISRGVFRYLERLVSHSVNLHVLTRLREDFYKRVEPG
                     APANLFSQQGGDMLQRVMGDLETLENFYIRTIAPVVIALVILAGVSLFIGGYALQLGL
                     IMAAGMGISGFIHPALAVFISRSPIIQLSRSKAAASARLVETLQGLEDIQVYNAQAPY
                     LEALLGEFRWSAVLQKRLVNLNAVSSGLALLFTHLTVLLLLWVGIPLVSAGQFSGISL
                     AVITLVALASFEAVSAMPAAALHLNASAAAANRQLTIGEEMSVYPRLTKTPRNIQRPE
                     LAFEAVRFTYPNAQRPTLEDIYFKITSGCKTALVGASGAGKTSLVNLLLNFEQPQEGS
                     IQLNGVKLAEMDAEESRSWFAVLPQSVYLFNDSLRGNLALAKADASDEELISALRAAC
                     LSAWYESLPEGLDTWVGEHGVKMSGGERQRLAMARVLLQDRAFIILDEPTANLDPATA
                     RKMMDQLFTRSPNKGLLLITYDLGLLREMDEILVLSEGTITQRGRMDSLLKAGGEFKH
                     IFEMENNKIRDS"
     gene            complement(8012..9754)
                     /gene="cydD"
                     /locus_tag="PLW25_05660"
     CDS             complement(8012..9754)
                     /gene="cydD"
                     /locus_tag="PLW25_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HOS54011.1"
                     /translation="MRIHPRLFKEARKNGLLFLLTALASFLAGGIAVIQSYQLSRVIS
                     GVLIDQRTLKEVSSILLFILLLIFSRVVLTIISEALAGRMTIAIKQRLRAELLDKVNR
                     LGPEFLKNEKTGELTTTALQGVDALDAYFSQYLPQVLISVALPLTILAVVFPMDLLTG
                     IIFVFTLPLIPVFMVLIGWASEALTKRQWKALTYLGNYFLDTLQGIATLKMLGRSKER
                     GNEVRDASERYRLATLAVLRVTFISALALELIATISTAVVAVEIGLRLLYGRIAFQQA
                     FFILLIAPEYYLPLRNLSARYHAGMTGVTAAQKIFEILDVPEPARQPVLSIENLEQVF
                     RTDYRLRVSNLYYSYPGMGSHSIQDISFCMQKGLHYALAGRSGSGKSTLARILLGFIE
                     PQEGQIWVNGSQVQAYSLEDWRRFVGWLPQTPFIFNDTLFNNVTLRDTRFSTSDVERA
                     LRLAGLAQLLATLPLGLHTPLLEGGARFSGGQLQRVALARIFLRQPSLLVLDEPTSHL
                     DPGLARALDDTFKTLLQDRTTLTIAHRLSTIAQADEVIFLKDGQIGAIGKHATLLETC
                     PDYGAFIHGSGVLA"
     gene            complement(9764..10453)
                     /locus_tag="PLW25_05665"
     CDS             complement(9764..10453)
                     /locus_tag="PLW25_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOS54012.1"
                     /translation="MLLGEMTWMEVEQYLKRDDRIMLVTGATEQHGYICLETDIKIPL
                     TLASHASERTGVLVAPPLNFGCSSYFVDFPGTISFRVQTLINAVEDMVRSVHHQGFKR
                     ILFVNGHGGNDPAKLALGELVNELPDLQVSWYDWWKSSSVAAVAEAHGLGMYHASWME
                     AFPFLRRSGMPDGIKEAFKPLQILPSKKVRAALVDGVYGGAFQASDEIMNEVFNAALQ
                     DVLGLLEFKMS"
     gene            complement(10575..>11774)
                     /locus_tag="PLW25_05670"
     CDS             complement(10575..>11774)
                     /locus_tag="PLW25_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOS54013.1"
                     /translation="ATNYRLRDWLISRQRYWGAPIPVVHCPNCGAVTVPEDQLPVRLP
                     DDVEWKPTGESPLKLHPTWRFTTCPQCGKPAERETDTMDTFVCSSWYHLRYLSPDYDK
                     GPFDPQEYDYWMPVDIYTGGIEHANMHLIYTRFFHKALRDMGLTKGNEPMLQLRNQGM
                     VLGEDSEKMSKSRGNVVSPDDLVHTYGADATRAYLMFFARWDMGGPWSSGGIDGVMRW
                     LRRAWSLVLEPGQTGAVNEESVKTLRRKVHQTLRSVSRDFEQFEFNTIISSLMELLNE
                     MAHLKDSVYGSDAWKEAIEIYLQMMAPVVPHIAEELWQRIHKPYSVHTSRWPSVDEKA
                     AADEEITLVVQLNGKVRDRITVPVDISEEEAKTRALKSDIIQKQMEGKEARQIIYVKG
                     RLINIVV"
     assembly_gap    11775..11874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11875..12809)
                     /locus_tag="PLW25_05675"
     CDS             complement(<11875..12809)
                     /locus_tag="PLW25_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOS54014.1"
                     /translation="MACQVPEYIPAEIEPRWQRQWEADGIYHADIDKKRPKFYALTML
                     PYPSGDLHIGHWYAISPSDARARYKRMNGFNVMFPMGFDAFGLPAENAAIKHNIHPRK
                     WTYANIQKMREQFRSMGTMIDWRREIVSSDEKYYRWTQWFFIQLVKHNLAYQKLSPVD
                     WCPHCNTTLAREQVWGDDRHCERCGTPVIKKDLKQWFFKTTRYADELVNYDGLDWPER
                     VRTLQTNWIGRSEGALVTFKTEAGDALEVFTTRPDTLWGVTFMVLAPEHPLVEKLTTI
                     EQRAEVDQYVLDASRQSDIQRESTEKEKSGIFTGG"
     gene            complement(13084..13156)
                     /locus_tag="PLW25_05680"
     tRNA            complement(13084..13156)
                     /locus_tag="PLW25_05680"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13121..13123),aa:Ala,seq:ggc)
     gene            complement(13195..13267)
                     /locus_tag="PLW25_05685"
     tRNA            complement(13195..13267)
                     /locus_tag="PLW25_05685"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13232..13234),aa:Ala,seq:cgc)
BASE COUNT         3276 a         3674 c         3294 g         3091 t
ORIGIN      
        1 atgatgactt cctcgatctt acgatgttca attatcttcc cgatatcggc gagtgaccca
       61 acaaccggta tgccatggat ttgctgctta agtttcgcag ggttgtcatc taaaaaccct
      121 accgggttga gattgagatc agggtttttc tgcagttcac gcaccaccag cgcgccggcg
      181 tctcccgcgc caatgatcaa cgcccgcttg gcaggccttt ttttcataga attggctgcc
      241 gtgctgacgt tatccgcgat aagacggatg gcaaagcgtg taccgccgac aagaaatatc
      301 gaaagcagcc agtcgatgat cagtaccgaa cgtggaaacc cgttaaaaac tttgaaggtc
      361 aacagcaaaa tcatcactgc tgaaaccagc acagaggcgc tggttacagc aagaaagatc
      421 aagcgtaatt ccctggtgct ggcgtatccc cacaaacgcc ggtataggct gaacaaataa
      481 tagatcggta tcttaatcaa caaagaaacc gcgatcatcc agtaagcaga aggcaggtag
      541 aaaaagaaaa gcgctccaag ttccagccgc aacgcataag cgcctaacac cgcgataatg
      601 gtaagcgcca tatcgatcat gaccatatat ctgttgcgca tgttgaattt tattctcatt
      661 aaagtccttt gaatcccaga accattccaa ccgttctgag gatgatggtc aaatcaagta
      721 ataggtttcg gtttttgata tagtatagat cgtattccag tttgaccgcg gtatcttcga
      781 ccgttgtggc ataacgctgg ttgatttgcg cccagccggt gagcccgggt cggacaaata
      841 agcgcgcgcg gtagaagggg agttcctgtt gaaatttgtt gaccagttcg atctgctctg
      901 cgcgcggacc aataatactg ttctcacccc ggaggatatt ccagcattga ggtaactcat
      961 ccacgtggct gcggcgcagg atctttccaa agcgggtaat acgggcatca ttggctgaag
     1021 ctgttcgcgc cacgccatcc ttttctgcgt cctggtacat ggtacgaaac ttaaatatct
     1081 tatagggatg cccatttttc ccgacgcgca tctgggagta gagtatggga aacccggaat
     1141 caatgatgat agaaagcgca atcagggggt acagcaaaac cagcaggagc aaaccaacaa
     1201 tactgcctgc gatgtcgatc aggcgcttga cgctttcata tacgccgctg gtgtgcgttt
     1261 ggtcaataaa agaacgtaat atccaatcgg tttgcagtaa aaatatcggc accctgccaa
     1321 aaacttcctc gtaaagcttc ggcagcgaag taacctcaat atcgctctca gccgcgttca
     1381 aaatagcgcg aaacaattca ggtttcaact cccccgagat ggcaaatatc aggtcggtga
     1441 tatcctgttc accgatcaca gccgtcatcc tttccgctgt ccccagcacg gggtaaccgc
     1501 tgatttcttc cccctgtttt tcaggatcat catcgatata accaacgagg atgaacggcc
     1561 ccgggtggat ttcacgcagc atggaagcca gtgttgaacc agccttgcca gcgccaatca
     1621 tcaaggcgcg gcgcatgaaa accggggcag tgaaaattcg aatgtaaagc gaacgccagg
     1681 cgaaagtgag aatggttgcg gcgataataa agataaagac gccccgccgg ggtagcgagt
     1741 ttggatcaga aatgaaaaag atgaccaggt aaatgataaa actgatcccg gctgcggtgg
     1801 ctacccctcg aatcacatcc ttccggtggt tggctttgcg gatatcgtaa acttcgatca
     1861 agaaaatgac ccagagaaac ggcagcaggt agaaccagaa cggagcgcgt tgccccagga
     1921 aggcccatga gaagtcgagc caatcttttt gcccccagaa gtacaaccct acaaaaaccg
     1981 cgaggtaagc tatcagcata tcgccaagaa acaggatcgc ctgtctctcg ccattgcgga
     2041 tgcgccagcg tttttttatt tgaggctttt caacggtcat gctcagagcc tttaaccaat
     2101 gacttcaaca tactccacaa aaaaccgctt ccccaggaaa aatgcatggt cataatggca
     2161 agcggtaccc ctggaatcag gctggatctt ttttgctgga tggcaggctg gatcgtgcca
     2221 gcaacaagaa tcgccaggta gaggcttgcc actcctgcca ataaaatccg cgcgggtatc
     2281 cagaatgtgg ataataatga taacatcaaa atacctgaaa caaaaaccgg gggcagcgcc
     2341 tgccgccagc gtagcgaatc gggatgacgc ctcaacattt tatacttcca gtaaccatag
     2401 gagaagtatt gcctggccag cgagcgcagg tccggcctgg aatagtagtc taccctaatc
     2461 gccggatcaa cccatatttt cccgcccgag cgcctgacgc gcgtgttaaa ttcataatct
     2521 tcattgatac gcagggattc atcgtagccc ccaatgcttt tcaccacgtc tcgcctgtat
     2581 gtgccaaatg caaccgtgtc ggttacaccc gctttcgtcg ccgtgcggta atgagcatca
     2641 cctaccccca gtgggtgcgc ggtcgccaca gcgatggcgc gcccaaccca ggtgtcgctt
     2701 ccagcgcgaa tattaatcac ccctcccaca ttctccccca ggccagcgtt taaagccctg
     2761 aggcattttt cgatgtaatc cggcgctggt atggcgtgcg cgtccatacg gcttaacacc
     2821 gcccccgtag aagcctgtat tgcctggtta agtgccgccg ggatggtgtg ctggtggtta
     2881 tccaccactc ttatggtcag ttgcggatgc ccggctttga aggcggttat cttctcgcgg
     2941 gtgcggtctg ttgacattcc atccgcgatg atgacctcca taaggtcaat tgggtaagtt
     3001 tgagcgagga gcgcgctgag cagttcctcg atcgtcgctt cctcgttata gcaagggatg
     3061 atgaccgata ctttaaccgg ttccatcggt gggtcaaaaa tcctattctc agcctgttat
     3121 gcgcaatcgc cgtctttaca caacagcgcc accacctcaa tttcgataag cgcatctttg
     3181 ggtaaacccg cgaccgctat cgtggtgcgc gccggcgggt tggaagggaa ataaccgccg
     3241 tacactttgt tcatagcggg aaaatctttc atgtcgcgca ggaaaacagt ggtcttcaca
     3301 acatgttcca ggcaagaaga cgctgacctt aacacagcgg ataggttgga aaagacctga
     3361 tgcgtttgtt cttcgatacc gcctgggaca agattgcccg tttcagggtc gatgccgatt
     3421 tggcctgcgc tgaacacaaa tgggccgcca ccggccgcaa cggaatatgg gccgatagcc
     3481 gccggcgctt cgctgctctg gtataccttc ttatcagtac atgccatcat tacctcctgt
     3541 gatttttcta cctcctcatt ttaagcgaac tgtaagaaaa ggtaaatgnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga ttctaattat tcaggaatgc catgctcgaa
     3721 aaaatcacgg ctatcgaaaa ccgctataac gaattgaccc gcttgatgga tgagaatatc
     3781 caggattacc aaaaagtggt ggaactcgcc aaagaacgtt cagaactgga gccggtcatc
     3841 cagctttcag cccgctaccg ctctctgctc aaccaattgg aacaggcgaa agaattggaa
     3901 tcaagcggcg atgaagaaat gcgcgcgctc gccaatcttg aaattgaagc gctccctcct
     3961 cagattgaag agctggaaaa aaagctgaag cgcctgctca ttcctcgcga cccgcgcgat
     4021 gatcgcaacg tgttcatcga aatccgcgcg ggcgccggcg gtgacgaagc ggggttattt
     4081 gccgcagagt tgatgcgcat gtacatgcat tacgccgaag cgcgcggctg gagcgtggaa
     4141 atgatttctt ccaacgaaac gggcatcggc ggattcaaag aagtgatttt catggtcaaa
     4201 ggcaaagggg cttattcgcg ttttaaattt gaatccggcg tgcaccgcgt tcaacgcatt
     4261 cccgccaccg aatccagcgg tcgtatccac acttcaacgg taacggtcgc ggtcatggca
     4321 gaagtagaag aagtagatgt tcagatccct gaatccgaca tcaccattga tgtatttcgg
     4381 tcttccggcg cgggggggca aaacgtgcaa aaaaacgcca ccgcggtgcg tatcacgcat
     4441 atccctactg gtatcgttgt cgcctgccag gatgaaaggt ctcagttgca gaacaagctc
     4501 agggcgatgg gcattcttcg cgccaggctg tttgaaattg aagaggaaaa acgccgccaa
     4561 gagcgcgatg aaaaccgccg ctcccaggta ggtaccggag atcgttcaga gaaaattcga
     4621 acttacaatt acccgcaaag ccgggtgacc gatcaccgca tcaacctttc ttcttataat
     4681 ttacccgccg tgatgaccgg cgaaattgac ctcttcattg acgaactcgc tctccgcgat
     4741 gagacagacc gcctctcttc aagcggcgat tatgacgaga gttaatctgg gccgctggat
     4801 cgcccaaaaa cgagcgcagt tgcagggaaa aacggatttc ccttccatcg aaatcaacgc
     4861 cattgcttct catctcttac aacaacctac tgcatgggtt atttctcatc ctgaaacggt
     4921 gctcactgac gcccaatatc aattaatgga agacgcggtc aaccgtctgc tgttgggaga
     4981 accccttgcg tacatcaccg ggcacaagtc cttttttggg ctggatttca tcgtcgacaa
     5041 ccgggtgctc attccccggc cagaaacaga aatactcgtg gagcacgccc ttgcctggct
     5101 gaaggcttat cccgggaaga gaaaagtgat cgatatcggc actggctcag gcgctatttc
     5161 catcaccctg gtaaaaaatc tggctggttt gcaggttacc gcggtggata tttccgctgc
     5221 tgcactggcg gttgccaccg ccaacgtcca ttaccataaa gtggcaaatc agatccgttt
     5281 gattgaatca gacctgtttg aacaggttga tcaaaaattc cacctggtgc tggccaacct
     5341 tccctatatc ccatccattg atctcaattc gttaagcgtg gttaagtacg agccgcggga
     5401 agccctggat ggcggttctg atggaacgga actcattacc agactgatcg atacaatgcc
     5461 acaccatctg ttgccaggtg gatgtgcaat tctggaaata caatataatc aagataacct
     5521 ggtcagcagc atcgccgctc gtcgcttccc tgacgccagt atttccttca tcaaggactt
     5581 ggcttctatt aatcgttttc ttaaaatcca gctgtgaaac aggttattca atgcagacca
     5641 taaccttacc cgcagataac cccgcttcca tcgaaaccgc tctcgacctg ttaacagagg
     5701 gagaaatcgt ggctttccct accgatactg tttacggtct gggagccaac gccttctatt
     5761 ctccaggcat tatcaagttg tttgaagcca aggggcgcga cgccaataaa gccattgctg
     5821 tgcttatcgg agataacggg catttggatt tgctcacaga tttcctttct gccaacgcgc
     5881 gtaaattaat taagcacttt tggccaggcg ggctaaccat cgtggtacca aaaaagaaag
     5941 acctcccaga gttactctct gcgggaagct caatcggtat ccggatgccc aatcacaaca
     6001 tcgcgcttga gctgctgaat aaatttggtc ctctcgctac cacttcagcc aacctgagtg
     6061 ggaagaataa cccgcataat gccggagatg tcttgaacca gttgaacggg cgggtcccgc
     6121 tcattttgga tggcgggcgc tgccccgggg gcgttccctc caccgttgtg gattgctcta
     6181 cggatgaggt cagaatcctt cggcccggcg cgatttcaca cgaagccatc gacgcggtaa
     6241 tatcccgatt ataaacctgc acagtttgcg gttggcactc acgaatcgcg aattttgttg
     6301 ttttccattt caaagatatg tttaaattcc ccgcctgctt tcagtaatga atccatcctg
     6361 ccgcgttgtg tgatggtacc ctcgctaagc accagtattt catccatctc gcgcagcaat
     6421 cccaaatcat aagtgatcag cagcagccct ttgttgggcg acctggtaaa aagctgatcc
     6481 atcatcttcc gcgcagtagc agggtcgagg ttggcggtgg gttcatcgag gatgatgaac
     6541 gctcgatcct gcagcagcac gcgagccatc gccagccgct gccgttctcc accgctcatt
     6601 ttcaccccat gttctccaac ccaggtgtcc agcccttccg gcagcgactc gtaccatgca
     6661 gaaaggcatg ccgcccgcag cgcgctgatc agctcttcat cgctcgcgtc cgcttttgcc
     6721 agcgccaggt tgccgcgcag ggaatcatta aatagataca ctgattgcgg taacaccgcg
     6781 aaccaggacc tgctctcttc tgcatccatt tcagcaagct tgaccccatt cagttggatg
     6841 ctcccctctt gcggctgctc aaagttaagc aataaattca ccaggctggt tttccctgca
     6901 ccactggcgc caaccagggc tgtcttgcag ccgctcgtta tcttaaaata aatgtcctcc
     6961 aaggtaggtc gttgagcatt cgggtaggtg aaacgcactg cttcaaaagc cagttctggt
     7021 cgctgtatgt ttcgcggtgt tttagtgagc cgcgggtaga cgctcatttc ttcgccaatt
     7081 gtcaattgcc ggttggcggc tgccgcgctg gcattgagat gaagtgcagc cgcgggcatc
     7141 gcggataccg cttcaaagct ggctaacgct accagtgtga tcactgcaag cgaaatgcca
     7201 gaaaattgcc cggcgctgac caggggaata cccacccata acaataacaa cacagtcaga
     7261 tgggtgaaca gtaacgccaa cccgctgctg acagcattga gattcaccag gcgcttttgc
     7321 agcaccgcag accatcgaaa ttcacccagc agcgcttcaa ggtatggcgc ctgggcgtta
     7381 taaacctgga tgtcttccaa tccttgcaac gtttcaacca acctggctga tgccgcggct
     7441 ttcgacctgc tcagctggat gatggggctt cgtgaaataa aaaccgctaa cgcggggtga
     7501 ataaacccgc tgatacccat cccagcagcc atgatcaaac ccagctgtaa agcataacca
     7561 ccgatgaaaa ggctcacacc agcgaggatc accagagcga tcacaacagg ggcgatagtg
     7621 cgaatataaa aattctccag cgtttccagg tctcccatca cccgctgcag catatccccg
     7681 ccctgttgag aaaataaatt tgccggcgcg ccgggttcaa cacgcttgta gaaatcctcg
     7741 cgcaacctgg tcaacacatg caggttgaca gaatgcgaaa ccaaccgttc caggtagcga
     7801 aaaacgccgc ggctgatacc gaagaagcgc actcccacga ttgccacctg taattccgcg
     7861 atcgaaggtt gaaaggctgc gctggcaatg agaaaagcag aggtacccaa caacccgatc
     7921 cccgcggcaa cggcagcaac accaagtatc agcgagagaa agacctgccc tgagaattcc
     7981 ttaaggatga tgcaaactcg ccaaagccct ttcacgccag tacccccgac ccgtgaataa
     8041 acgcgccata atccgggcag gtttccagta aggtggcgtg ctttccaatc gctccaatct
     8101 gcccgtcttt aagaaagatc acttcatctg cttgcgcaat ggttgaaagc ctgtgcgcaa
     8161 tggtcaatgt cgttcggtct tgcagcagag tcttgaacgt atcatccagt gcacgcgcca
     8221 atccagggtc aaggtgcgag gtgggctcat ccaacacaag caatgagggt tggcgtaaga
     8281 agatgcgcgc caatgccacg cgctgaagct gacctccgct gaagcgggcc cctccttcca
     8341 gcaacggagt atgcaacccc agcggcagcg tggcaagcag ttgagctaag ccggccaatc
     8401 gcagggctcg ctccacatca ctggtagaaa accgggtgtc tcttaaagtc acgttgttga
     8461 acagcgtgtc attgaagatg aagggggttt gaggtagcca gccaacgaac ctcctccagt
     8521 cttccagtga atacgcctgc acttgtgacc catttaccca aatttggcct tcctgaggct
     8581 caataaatcc caacaggatg cgcgctaaag tgcttttccc agaaccgctt cgacccgcca
     8641 gggcataatg cagccctttt tgcatacaga aagaaatatc ctgaatggag tgagaaccca
     8701 ttcccgggta agaatagtac aggttactga cccttagcct gtaatctgta cgaaagactt
     8761 gctcaaggtt ctctattgaa agaaccggct ggcgagcagg ctcaggaaca tccagaattt
     8821 caaatatctt ttgcgccgca gtcaccccgg tcattccagc gtggtatcgg gcgctgaggt
     8881 tgcgcagggg aaggtagtac tcaggggcga tcaacaaaat aaaaaaagcc tgttggaatg
     8941 cgatacgccc gtaaagcagc cgtaacccga tctccactgc caccacggcg gtggagatcg
     9001 tggcgatcag ttccagcgcc agcgccgaaa tgaatgtcac ccgcagcacc gccagcgtgg
     9061 ccaggcgata acgttcgctg gcatcgcgta cttcgtttcc ccgctccttg ctgcgtccga
     9121 gcatttttaa tgttgcgatc ccctgcaatg tatccagaaa ataattcccc aggtatgtga
     9181 gcgctttcca ttgtcgcttt gttagcgctt ctgatgccca gccgatcagg accatgaata
     9241 ctggaatcaa aggcagggtg aaaacgaaga taatccccgt taaaagatcc attgggaaca
     9301 ccaccgccag gatggtcagc ggcaaggcca cggagattaa tacttgcggg agatattgcg
     9361 aaaaataggc gtctaacgcg tcaaccccct gaagcgcggt ggtggtcagt tctcctgttt
     9421 tttcattttt aagaaactca ggccccagcc gattgacctt atccaggagt tcagcccgta
     9481 atctttgctt gatcgcgatc gtcatcctgc cggccagcgc ctctgaaatg atggtcaaga
     9541 ccacacgaga gaagatcagc aagagaatga acagcaggat cgaactgact tccttaagcg
     9601 tgcgttgatc gataagcacg ccggaaatga cgcggctcag ctggtaggat tggattaccg
     9661 cgatccctcc cgccaggaaa gaagcaaggg ctgtcaggag aaataacagc ccgtttttcc
     9721 tcgcttcctt gaaaagacgg ggatggattc tcatgcggat gcgttagctc atcttaaact
     9781 cgagtaaccc cagcacgtcc tgcagcgcgg cgttgaaaac ctcattcatg atttcatcgc
     9841 ttgcctggaa tgccccccca taaaccccgt cgaccagggc agcgcgaact ttctttgaag
     9901 gcaggatttg aagcggtttg aaggcttctt tgataccatc cggcataccg cttctcctta
     9961 aaaatggaaa agcttccatc caggacgcat gatacatccc caatccgtgt gcttctgcca
    10021 cggccgccac tgaggaggat ttccaccaat cgtaccagct cacctgcagg tcaggtaatt
    10081 cattcacaag ctctcccagc gccagtttgg ccgggtcatt cccgccatga ccgttcacaa
    10141 acaggatgcg tttgaacccc tgatgatgca cagacctgac catatcttcc actgcgttga
    10201 tcagtgtctg cactctgaag ctgatggtgc cgggaaagtc gacgaaatac gaggaacagc
    10261 caaaattcaa cggcggtgcc accaacacgc ctgttctttc tgaggcatgg ctggccaggg
    10321 tcagcgggat cttgatgtca gtttccaggc aaatatagcc atgctgttcg gtcgcgccgg
    10381 tcaccagcat gatccggtca tctctcttaa ggtattgctc cacttccatc caggtcattt
    10441 caccaagcag catattcacc tcgctttttc ttcttgatag tcaagttcat tataatggaa
    10501 cacgcgctgg aagttaaaaa aaaactggct gcgctaaaag ccggcagcca gcatacttct
    10561 tctcttcgtg cggctcacac cacgatattg atcagccgcc ctttgacata aatgatctga
    10621 cgggcttctt taccctccat ttgcttctga ataatgtcgc ttttaagcgc gcgggtttta
    10681 gcttcctctt cgctgatatc taccggtacg gtgatacggt cccgcacctt accgttcaac
    10741 tgcaccacca gggtgatttc ttcgtccgcg gcagccttct catccacgct tggccagcgg
    10801 ctggtatgaa cggaatatgg cttgtggatt cgctgccaca gttcttctgc aatgtgcggc
    10861 accaccggcg ccatcatctg aagatagatc tcaatggctt ctttccatgc atcactacca
    10921 taaacactat ccttaagatg cgccatctcg ttcaaaagtt ccatcaggct ggagatgatg
    10981 gtattgaatt caaactgctc aaagtccctg gaaaccgagc gcagggtctg gtgtactttt
    11041 cggcgcagtg tttttaccga ctcctcgttg accgcgccag tctgcccggg ttccagaaca
    11101 aggctccacg cgcgcctcag ccagcgcatc acgccatcaa tccccccgct gctccacggt
    11161 cctcccatgt cccagcgggc gaagaacatc aggtaagcgc gcgttgcatc cgccccatag
    11221 gtgtgaacca ggtcatccgg agagaccacg tttccgcggc tctttgacat cttttctgaa
    11281 tcttctccca gcaccatccc ctggttgcgc agttgcagca tgggctcgtt gccttttgtg
    11341 aggcccatat cgcgcaacgc tttatggaaa aaacgggtgt agataaggtg catgttggca
    11401 tgctcaatgc cgccggtgta aatgtccact ggcatccagt aatcgtattc ctgcggatcg
    11461 aaaggacctt tatcgtaatc cgggctgagg tagcgcagat gataccagga tgagcacacg
    11521 aaggtatcca tcgtgtcggt ttcgcgttct gcgggtttac cgcattgcgg gcaggtggtg
    11581 aatcgccagg tagggtgcag ttttaagggg ctttccccgg ttggcttcca ctccacatca
    11641 tcaggtaatc ggacggggag ctggtcttca ggcacagtga cggcgccgca attcgggcaa
    11701 tggacaacgg ggataggcgc gccccagtaa cgctgccgtg aaataagcca gtcgcgcagg
    11761 cggtagttgg tggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntatccg
    11881 ccggtaaaaa tgccgctctt ttctttttct gtcgattcgc gttgaatatc tgattggcga
    11941 gaagcatcca aaacgtattg atccacttct gcccgttgtt caatggtggt cagcttctcc
    12001 accagtggat gttcaggcgc caataccatg aacgtgaccc cccacagggt atccgggcgg
    12061 gtggtaaaaa cctccagcgc atctcctgct tcggttttaa aggtcaccag cgctccttct
    12121 gacctgccaa tccagttcgt ctgcagcgtg cgcactctct caggccaatc aagcccatcg
    12181 taattgacca gttcatcggc gtaacgggtt gtcttgaaaa accattgttt aaggtctttt
    12241 ttaatgaccg gtgtgccgca acgctcgcag tggcggtcat ctccccacac ctgttcccgc
    12301 gccagggtgg tattacagtg ggggcaccag tccaccggag atagtttctg gtaagcgaga
    12361 ttgtgtttga ccagctggat gaaaaaccac tgcgtccagc ggtagtattt ctcatcagaa
    12421 gacacgatct ccctgcgcca atcgatcatc gttcccatac tgcggaattg ttcacgcatt
    12481 ttttggatgt tggcgtaagt ccacttacgg gggtgtatgt tgtgtttgat ggctgcgttc
    12541 tcagccggca agccgaacgc atcaaaaccc attgggaaca taacattaaa gccgttcatg
    12601 cgtttgtaac gcgcgcgcgc atcagagggc gaaatagcat accaatgacc aatgtgaagg
    12661 tcacctgaag gataaggcag catggtaaga gcgtaaaact tggggcgctt cttatcaata
    12721 tccgcatgat aaataccgtc tgcttcccat tggcgctgcc acctgggctc aatttctgcg
    12781 gggatatatt ctggaacttg acaggccatt cttctctcca atcaaagcat taaaataaaa
    12841 aaccttcttc cctacaggga cgaaggaatc ctccgcggta ccaccctgct tgcctgcagt
    12901 tgacgcagac cgcttaatag cgctaacggg cttacccgtt accggttact gcacttcacc
    12961 ggaaaagcta tcctctcgga tttcaggcga gttcggtcct tgtgttccgc aacacacgcc
    13021 gggctcgcac ctcgctctcc cggctcgctg tcggatgacc tactactcct gcgttcgctc
    13081 tagtggaccc agggggattc gaacccccgg ccttctcaat gccattgaga cgcgctccca
    13141 actgcgctat gggcccggat attacccgtt tgttgctcga gcctgccgtg ctagtggacc
    13201 tggggggatt cgaacccccg gcctcttcag tgcgattgaa gcgcgctccc aactgcgcta
    13261 caggcccgtt cacaaaggta gccagatttt aatcgagaca tggcgcagtg tcaagaaaga
    13321 aattggctaa agaaatttcg gatgtatgct tttctccaat cctgcttcag gctataatcc
    13381 tcttattcag gagttcatcg atgacagaaa aaagattcgc ccacttacac gaattaatga
    13441 agcaacacca gcttaaaacc atcatcttga acccgggacc cacgcttgct tacctcaccg
    13501 ggttggattt tcacctgatg gaacgaccaa ccatg
//
LOCUS       DAOSPM010000049        13514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_4578_length_13514_cov_11.710974, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000049 DAOSPM010000000
VERSION     DAOSPM010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13514
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4578_length_13514_cov_11.710974"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..234)
                     /locus_tag="PLW25_05690"
     tRNA            complement(163..234)
                     /locus_tag="PLW25_05690"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(200..202),aa:Met,seq:cat)
     gene            complement(298..1002)
                     /gene="radC"
                     /locus_tag="PLW25_05695"
     CDS             complement(298..1002)
                     /gene="radC"
                     /locus_tag="PLW25_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOS54015.1"
                     /translation="MTRENGATYRIKDLAEDERPRERLASIGAEFLSKAELLAILLRV
                     GTEGTSAVELGSQILKELGDLRGIQRASLAEMCRIRGLGPAKAAQIKAAIELGRRLTL
                     EAPQLAAGISSPADAAELVRYEMQALLQEELRVILLDTRNRKIDVKKVYTGSLNSSMV
                     RVGELFREAIQRSAASIILVHNHPSGDPSPSPEDTVLTRSVVQAGKLLDIEVLDHLVI
                     GQGSFVSMKEKGLGFE"
     gene            1118..1648
                     /locus_tag="PLW25_05700"
     CDS             1118..1648
                     /locus_tag="PLW25_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOS54016.1"
                     /translation="MSLDPSFLKQLTLFAIGFAAAFLAALWLSLIFWASRDIRQRSRD
                     RFLRILAVVIVALLSIPGFLIYLILRPARTLEEEYQSALEEEALLQAIEESALCPGCN
                     RRIHADWLVCPSCNTVLKKTCKKCGKLLELPWNICPWCASAIPGTRKPTDKKKEEPEL
                     DEGFQPLLDEPLDTHP"
     gene            1667..2530
                     /locus_tag="PLW25_05705"
     CDS             1667..2530
                     /locus_tag="PLW25_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS54017.1"
                     /translation="MPDLATIEPVDYLIIGHITQDVTPAGLSLGGTASYAALTARAFG
                     LRVGIVTSCSPDLQPPELNGIAIARKPSPFTSTFRNTYTPTGRIQHILHVADLLSLED
                     VPPAWRSAPIVHLGPLANEIPANLVRAFPRSLVGVTPQGWMRAWDGDHIVSYRYWVES
                     DQVLPHVQSCVMSVEDVRGDEEAISHFAALAPILVVTEGAAGSRVYWNGDVRFFRPPL
                     TQEVDPVGAGDVFAASFFIKLLQTRDPWEAARFATLVGANSVTRVGILGVPTPSELRA
                     YSTQIIAHPKQ"
     assembly_gap    2583..2682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2691..3482
                     /locus_tag="PLW25_05710"
     CDS             2691..3482
                     /locus_tag="PLW25_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS54018.1"
                     /translation="MLTLVNQKGGVGKTTTAINLSAYLGLFSKRVLLIDLDPQANATS
                     SLGINKYEVKNGTYEVLIGEQPILPNILLNPRLKIALLPSSPALAGSEVELIGMEGRE
                     FRMREALKPALDRYDYIIIDCPPSLSLLTVNGLIAARDGVLIPVQCEYLALEGIGQLT
                     QTLNLVRSSLFPEMRIRGVILTMFDIRTNLSTDVVNEVRKFFPNLVYDTVIPRSVRLA
                     EAPSYGLPISHYAPSSSAASAYAALTREILAQDGEPSPPFKEQVA"
     gene            3483..4331
                     /locus_tag="PLW25_05715"
     CDS             3483..4331
                     /locus_tag="PLW25_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOS54019.1"
                     /translation="MAQKTGLGKGLDALIPAGLNTTPTRADSYAPVSMIIPNPQQPRD
                     TISPESLAELSSSIREHGVLQPLVVSHDPVTGMYTLIAGERRLRAAKIAGLEKVPIII
                     RTVSEQQRLTQALIENIQRADLSPLETARGFRRLIDEFNLTHETLADQVGKSRVTVTN
                     TLRLLNLPAEAQEALAASRISEGHARALLGLPTPQAQLSALHTVLSQQLNVRQTEELV
                     RKLTGIKPRSLPPVEVDPHFQELEERLRVKLGTRVTLRSGKKGGTITIHYYSDEELNG
                     LIEKLT"
     gene            4689..5903
                     /locus_tag="PLW25_05720"
     CDS             4689..5903
                     /locus_tag="PLW25_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOS54020.1"
                     /translation="MSNEFLLDEIDKEKLLQFCHFVSSSDDWKATLDKMVTYIREFFI
                     FDNLVVYLGDSTIKRMDVLYARAMGRGRSKEADVSWGEDIASRVVAEKKAFIIQPENK
                     TASDRLQSPFLLGVPLSISKEITGVLIFIRFGGPDFEPGSKEFAELMGTLFASVIRQK
                     TINDYEKELSGLRRSSNLQDDFISTITHELRNPLGFIRGYTTTLLREDADWDEKTKHD
                     FLEIIDRETGHLGELIDDLLDSSRLQSGNMRFDFQSLHIDAIIRDQVKRAEYTNPSQV
                     IKLELEQQLPTVVGDPRRLAQVIDNLIENSKKYAPGSVITIKVFQKEANLVIEFSDTG
                     PGIPEKYLPNMFSRFFRVPELEKSVYGTGLGLSICKQIINNHNGEISVSSSQGEGIKF
                     TITLPLNHDVSL"
     gene            5913..6620
                     /locus_tag="PLW25_05725"
     CDS             5913..6620
                     /locus_tag="PLW25_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS54021.1"
                     /translation="MNARILIVDDEPLYLRLIKVNLEQEGYLIATAKNGQEALDLVSH
                     EMPDLIILDVMMPKLDGITTATRIRQFSNVPIIMLTALGEERDRVRGLNLGADDYVVK
                     PFSATELVARVKAVLRRSQLKENAGRSRILTHGDLKIDIAKAEVFKGDHPVYLSATEY
                     KLLIHFAHNLGKVLSAEELLTTTWGPGYKDEKEILWVSIARLRQKLEDDPHEPVHIVT
                     RTGMGYQMPALDEQAKK"
     gene            6683..7360
                     /locus_tag="PLW25_05730"
     CDS             6683..7360
                     /locus_tag="PLW25_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOS54022.1"
                     /translation="MEVSPLEEQSQLISARLLAAREVSEKTLARVAEQLGVSVKMVKN
                     YESGQSTPSLPQLEVLSELYRIPIENLLSGNPDLLKKTILPREKVNNFLEIRNRVIAA
                     TLKQQRLAQNWTIKKLAAAVGISAAILPRYESGETAIPAPILEHLCSQLGIPLKSLYS
                     SITLQNSEPPPIASPSQDSSQLPEEVQSFIQNPANLPYLELAKRLSGMDATKLRAIAE
                     NLLEITF"
     gene            7364..7900
                     /locus_tag="PLW25_05735"
     CDS             7364..7900
                     /locus_tag="PLW25_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54023.1"
                     /translation="MTAEIEKLKQQAIKDYNRRKYDAAIAGFQSCLEFYEKQNDELLV
                     AEMRNNISVALLGKNAAQEAYDIVKGTDETFQKVGDLKGQGMALANTAAALEALDKKE
                     EALALYEHTLDIFKEAGEKELRINILRRVSDLQLKTKRGFQALASMEAAYNQREKPTI
                     KDSVFKTTLAAIRNKLFK"
     gene            7901..8758
                     /locus_tag="PLW25_05740"
     CDS             7901..8758
                     /locus_tag="PLW25_05740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS54024.1"
                     /translation="MLQAPSNTEIRQAVSQDITAITDFVDKASLTHRHLDWRPLVEWV
                     SQPPFLLRFANQQLTALLSCAPDPEGVAWIHAFIMDHWSNQVNAVWHSLFEPALIELA
                     VTRCQVFSLSLSDWYTRLLRSSGFTPFENIVVLHWGREVPPVKNMPPEVLIRPMEPAD
                     LNQVAEVDRQTFEAAWVISRASLERAYLQSAHASVAEVNEQIIGYELSTASSLSSHLA
                     RLAVLPEFRHLNIGYSLVAQLLEYFTQRGIWQITVNTQDNNQASLSLYKKLGFFQTSE
                     SFPVFTKSG"
     gene            8839..9534
                     /locus_tag="PLW25_05745"
     CDS             8839..9534
                     /locus_tag="PLW25_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOS54025.1"
                     /translation="MTKRDVVQQRRKQNRSKNSLTTILIIAAFVFVVVGMVILTQYKP
                     VGQIATITRTTTAQKDGLSLGNPDAPVQVIEFADFQCPACANYWSVLEPQIIKDYVET
                     GKARFTYSPFSFLGQGQAWDESIKAAEAAYCANDQQKFWEYRDMVFANHNGENRGAYS
                     KERLVAFAKQLDLDMKAFNACFNSGKYSQQVINDNKFATEQGATYTPSFLINGKIVNA
                     AELVQAIETSLTN"
     gene            complement(9581..10285)
                     /locus_tag="PLW25_05750"
     CDS             complement(9581..10285)
                     /locus_tag="PLW25_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOS54026.1"
                     /translation="MTGTLINFAGILVGGLTGLLLGSRLKEKLKQTIISALGLFTLLY
                     AISLFLETQNALIVLGSLLIGILLGEWWRIEEGLMALGEKLEGRLNHSGRGEGSSRFI
                     QGFLSSTLLFCIGPMAVLGSLQDGLSGGSYTLIIKSILDAFAALAFASSLGAGVLFSS
                     VPVLLYQGSITLLAQQVQRVATPEMLNELSATGGVILAGLAISNIMEIKKIRTGNFLP
                     ALLVAPLLVFFLSLFK"
     gene            complement(10282..10839)
                     /locus_tag="PLW25_05755"
     CDS             complement(10282..10839)
                     /locus_tag="PLW25_05755"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOS54027.1"
                     /translation="MKKFYTGTGDKGSTGLLGEQRVDKFDLRMEALGTLDELSAVLGI
                     ARSMLTDPQDRQKIILYQRKLYQLMAEVAASPENREKFAKINEQSVNSLEAEIDRISM
                     RVDTPAGFILPGETPTSAYIALARTITRRAERRVAELTSRGDLTNAYVLIYLNRLSSL
                     FFAMELDLIQRETSHKPTMARESST"
     gene            complement(10846..11367)
                     /locus_tag="PLW25_05760"
     CDS             complement(10846..11367)
                     /locus_tag="PLW25_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54028.1"
                     /translation="MEDDIRLILAAGEELAQYLSSPIILWRVKHCNQPLSPGNLLLAI
                     TRLKTVNEIAVQHDEMNKVEKLIDTRRQAWENRARQEIPLRLNQYRELVDEYLEQGAI
                     DASYRSNISTRVKLDLLFRSLAAEPYVEQRKAQELDRQLGALLPEGDFIWEPELETGF
                     PKSIYAYLYVRKK"
     gene            complement(11384..13084)
                     /locus_tag="PLW25_05765"
     CDS             complement(11384..13084)
                     /locus_tag="PLW25_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOS54029.1"
                     /translation="MKKYRWQLLVLFLTGLVVGTLLLLEKRGGLGEVNTPQPAQGGVY
                     TEALIGYPQRMNPLLDSTNSVDRDVDKLIFSGLVKFDSRGIPAPDLAETIGISQDGIL
                     YNISLKPGLVWHDGEKLTTKDVLFTIELLRTGGVYIPEDIRKLWDSIEVYLFDDQHMQ
                     LKLPEAYAPFMDYLTFGVLPQHLFAGMDINEIAASAINLQPVGSGPYRFGELLMEGST
                     VSGIRLLANEDYAGGRPYLQEIVFRYYPDASSAYQAYVQGAVQGISEVPTDLLPQALA
                     NEDLRIYTGRLPRISLVLLNLNDPGVPFFKEIEVRKALMLGLNRQAIISQMFNGQAIV
                     ANSVILPGTWAYNESIAEIGYDPQLAAETLKNAGYVIAGENNTTRAKEEVPLSFVLSY
                     PDDDLHLGIAQLIANNWQALGVNVTLEGVPPDVFVAEKLEPRSYQAALVDLNLSRTPD
                     PDPYPFWDLGQAENGQNYSQWNNRLASDTIEQARVTTDLGERTRLYHNFQAIFAEELP
                     ALPLYYPVYNYAVSEQILGVTMGPLFDTSDRLGTITTWYLTTSRNQKTTDTAETDTGN
                     "
     gene            complement(13171..13395)
                     /gene="secG"
                     /locus_tag="PLW25_05770"
     CDS             complement(13171..13395)
                     /gene="secG"
                     /locus_tag="PLW25_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOS54030.1"
                     /translation="MELYIDIALIITSIALIASVILQNKGVGLGGLTGQDSGGVFTAR
                     RGIEKTLFWVTIVLSGLFFILTFAAVLIQG"
BASE COUNT         3392 a         3665 c         3151 g         3206 t
ORIGIN      
        1 aacacacact aagaaaggtt aatatttaag tggcgagggt gggacatgta ccctggcggg
       61 tatttgaaca cacaatgaga aaggttaata attgagtgac gagggtggga catgtaccct
      121 ggcgggtatt tgaacacaca atgagaaagg tttatattta agtggcgagg gtgggatttg
      181 aacccacatc caatggctta tgagtccact gctccaccat tgagctacct cgccagcgct
      241 ggttatctta ctataatcaa ccctgtttgt caaacaaaac cccttgaaaa tttactctta
      301 ctcaaatccc agtccttttt ctttcatcga aacaaagctt ccctgaccga tcaccaggtg
      361 gtcgagcacc tcgatatcca gcagcttacc ggcctgcacc accgagcgcg ttaaaaccgt
      421 gtcttcgggg ctgggtgaag ggtccccgct ggggtgattg tgcaccaaaa tgatcgaagc
      481 cgcgctgcgc tgaatggcct cacggaacag ctcgcccacc cgcaccatcg acgaattgag
      541 cgacccggta taaacttttt tcacgtcaat cttgcgattg cgcgtgtcca ataaaatcac
      601 gcgcagttct tcctgcagca atgcctgcat ttcatagcgc accagttccg ccgcatctgc
      661 cggagagctg atgcccgcgg caagctgcgg cgcttccagg gtcaacctcc gacccagctc
      721 aattgccgcc ttgatctgcg ctgccttggc cggtccaagc ccccggatgc ggcacatctc
      781 cgcgaggctt gccctttgaa tgccacgcag atcgcccaat tcctttaaaa tctgtgagcc
      841 cagttccacc gcgctcgttc cttctgtgcc tacacgcagt aatatcgcca gcagctcggc
      901 tttgctcaaa aattcggctc caatgcttgc caggcgttca cgcggtcttt catcttccgc
      961 caggtcttta atgcggtaag tcgctccgtt ttcacgcgtc atttcttcct cctagctgca
     1021 tttggaactg cttgttttat ataacaacac cccctttcac gcaagcgctt tcaccttcgt
     1081 ttctcaacca tgctataatt tcccacagga gccccgtatg agtcttgatc cctccttcct
     1141 taagcagtta actctgtttg ccattggttt cgcggcggct ttcctggctg ccttatggct
     1201 cagtcttatc ttctgggcaa gccgtgacat acgccagcgt agccgcgacc gctttttgcg
     1261 catactggca gtggtgatcg ttgcgctgct ttccatcccc ggtttcctca tttacctcat
     1321 cctgcgccct gcgcgcacgc ttgaagaaga ataccaaagc gcattggaag aagaagcgtt
     1381 gctgcaagcc attgaagaat ccgcgctctg cccgggctgc aatcgccgta tccatgcgga
     1441 ttggctcgtc tgccccagct gcaacacggt gcttaaaaag acctgcaaaa aatgcggtaa
     1501 actgcttgag ctgccctgga acatttgccc ctggtgcgcc tccgccatcc cgggtacgcg
     1561 caagcccacc gataagaaga aagaggaacc ggaactggac gagggttttc agccgttact
     1621 ggatgagcca ctcgacaccc acccctagac cggtacaaag ccatcaatgc cagatctcgc
     1681 aaccattgaa cctgtcgatt acctcattat tggtcatatc acgcaagatg tcacccccgc
     1741 agggctctcg cttggaggta cagcctccta cgccgcgctg accgcgcgcg cctttggctt
     1801 gcgcgtgggc atcgtcactt cctgctcccc tgaccttcag ccgccagaac tgaacgggat
     1861 cgccattgcg cgtaaaccat cccctttcac ctctactttt cgaaacacct atactcccac
     1921 agggagaatc cagcacattc tgcatgtggc tgacctgctt tcactggagg atgttccgcc
     1981 cgcctggcgt tccgcgccta ttgttcacct tggcccgctc gctaatgaaa tacccgcgaa
     2041 tctggtgcgt gcgtttcccc gttcattggt gggcgtaacc cctcaaggct ggatgcgtgc
     2101 ctgggatggc gatcacatcg tttcttatcg ttactgggta gaatcagacc aggtgttgcc
     2161 ccatgttcag tcctgcgtaa tgagcgttga agacgtgcgc ggggatgaag aagcaatcag
     2221 ccatttcgcc gcgctcgcgc caatcctggt ggttaccgaa ggcgcagccg gttcgcgcgt
     2281 gtattggaat ggagacgtgc gttttttccg ccctccgctc acgcaagaag tggaccctgt
     2341 cggcgccggt gatgtttttg ctgcgtcttt cttcattaaa ctattacaaa ctcgtgatcc
     2401 ctgggaggct gccaggtttg ctaccttggt aggggcgaat tctgtcaccc gcgttggcat
     2461 actgggagtt ccaaccccat ctgagctccg cgcttattcc acccagatta tcgcccatcc
     2521 aaagcaatga cacgcgtgct tacccttgtc aaccagaaag gtggtgtcgg caagaccacc
     2581 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgacacgc gtgcttaccc
     2701 ttgtcaacca gaaaggtggt gtcggcaaga ccaccactgc aatcaatctc agcgcgtacc
     2761 tggggctctt cagtaagcgc gtgctgctga ttgaccttga cccgcaggcc aacgccacct
     2821 cttcgctggg catcaataaa tacgaagtaa agaacggtac ttacgaggtg cttattggag
     2881 agcaacccat cttgcctaac atcctcttaa accccaggct aaaaattgcc ctgctgccat
     2941 cctctccagc gttggcaggc tcagaggtgg aactgatcgg catggaaggg cgtgaattca
     3001 ggatgcgcga agcgcttaaa ccagcgctcg atcgttatga ttacatcatc attgactgcc
     3061 ctccttctct cagcctgctc acggtgaacg gattgattgc cgcccgtgat ggcgtgctta
     3121 tcccggtaca gtgtgaatat ctggcgctgg aaggcattgg tcaactcacc caaaccctca
     3181 acctggtgcg ctcctctctc ttccctgaaa tgcgcatccg cggcgtgatc cttaccatgt
     3241 tcgatatccg caccaacctt tcaaccgatg tggtcaacga ggtgcgtaag ttcttcccca
     3301 atctggtgta tgatacagtc atacctcgct cggtacggtt ggctgaggca ccttcttacg
     3361 ggttgcccat ttcgcattac gcgccaagtt caagcgctgc cagcgcttac gcggcgttga
     3421 cacgcgaaat tctcgctcag gatggagaac cctctccgcc cttcaaggaa caggtggcat
     3481 aaatggcaca aaaaacagga ttaggtaaag gtcttgacgc gttaattccc gcagggttaa
     3541 acaccacccc aacccgggca gattcctacg cgccggtttc gatgatcatc cccaaccctc
     3601 aacagccgcg cgacactatc tcgccagaaa gcctggcaga attgtcctcc tccattcgcg
     3661 aacacggcgt cctgcagcca ttggtcgttt cacacgaccc tgtaacggga atgtacacgc
     3721 tcatcgccgg cgagcgccgt ctgcgcgctg ccaaaatcgc cggcttggaa aaggtgccta
     3781 tcatcatccg cacggttagc gaacaacagc gtctgaccca ggcacttatc gaaaatatcc
     3841 agcgagcgga cctatcaccg ctcgaaaccg ctcgcggctt caggcgcctg attgacgagt
     3901 tcaacctcac tcacgaaacc ctggctgacc aggtcgggaa aagtcgtgtc actgtgacaa
     3961 atactttgcg cctgctcaac cttcccgccg aagcacaaga agcgcttgct gcaagccgca
     4021 tcagtgaagg gcacgcgcgc gcgctcctcg ggttgcctac tccccaggca cagctctcgg
     4081 ccttgcacac cgtcctctca cagcagctca acgtccgcca aacagaagag ctggtgcgca
     4141 agttaactgg gatcaaacct cgctcccttc caccagtgga agttgacccg cacttccaag
     4201 aactcgaaga acgattacga gttaaactgg gcacccgtgt tacgctgcgc agcggaaaaa
     4261 agggcggcac catcaccatt cactattatt cggatgaaga actgaacggg ctcattgaaa
     4321 aactaacctg atcaatcaag accttctcac ccagcgttgc ctataacgat aacagagttg
     4381 ctttcattac aaggcagaac tttaaaggca agaacgcgcc ctctttcggc aacagaacac
     4441 caccccagtt ttctcgaagc gcaatgatgt cccctaaaac gcgagagtgt ttcaatccat
     4501 cttttaagcc acacacgcgg ttctcatcct cgctgtagct aaagaagaaa ggttttccag
     4561 tggttttcca ccctggcggg ggaatttcac gcgctacacg catttaaaag cgaaggttta
     4621 cgtaaaaacc gggcattcag cgagaaaatt gagaaccagt tggcacgaat attgtataat
     4681 ggttaatcat gtctaatgaa tttcttctcg atgaaatcga taaagaaaaa ctcttgcagt
     4741 tttgccattt tgtctcttca tctgacgact ggaaagcaac actcgacaag atggtcacct
     4801 acatccgcga gtttttcata ttcgacaacc tggtcgttta cctgggtgat agcaccatca
     4861 agcggatgga tgtgctgtac gctcgggcca tggggcgcgg cagatccaaa gaagccgacg
     4921 tttcctgggg ggaagacatc gcctccaggg ttgttgcgga aaagaaagcc ttcatcattc
     4981 aacctgagaa taaaaccgct tctgaccgct tacagtctcc ttttctgctc ggcgtgcctc
     5041 tgagcatttc aaaagaaatc accggcgtgc ttattttcat ccgttttggc ggtcctgatt
     5101 tcgaaccggg tagcaaggaa ttcgctgaat taatgggcac gctttttgcc agtgtcatcc
     5161 gtcaaaaaac aataaatgac tatgagaaag aactaagcgg gctgcgtcgt tcctcgaacc
     5221 tgcaagatga ttttatcagc accatcaccc atgagctgcg caaccccctg ggattcatcc
     5281 gcggttatac cacaaccttg ctgcgtgaag atgcagactg ggatgaaaaa accaagcatg
     5341 atttcctgga aattatcgac cgtgagacag ggcatctggg cgaattgatt gatgatttgc
     5401 tcgattcttc acgcttgcag agcggcaaca tgcgtttcga ctttcaaagc ctgcatattg
     5461 acgccatcat ccgcgaccag gtcaagcgcg ctgaatacac caacccatcg caggtgatta
     5521 aactggaatt agaacagcaa ctgccaacgg ttgtcggcga cccccgtcgc ctggcgcaag
     5581 tcattgacaa cctgattgaa aattcaaaaa agtacgctcc gggctcagtg attaccatca
     5641 aggtttttca aaaagaggcc aacctggtta ttgaattttc cgacaccggc ccagggatcc
     5701 ccgaaaaata tctacccaac atgttctccc gttttttccg cgttcctgag ctggaaaaat
     5761 ccgtatatgg taccggttta ggtttatcta tctgtaaaca aatcatcaac aaccacaacg
     5821 gagagattag cgtctcttct tctcagggag aaggcataaa attcaccatc acacttcctt
     5881 tgaaccacga tgtgagtcta taaggattga ttatgaatgc caggatactg attgttgacg
     5941 atgaacccct ctatttacgg ctgattaaag tgaaccttga gcaagaaggt tacctgattg
     6001 caaccgccaa gaatggtcag gaagcgcttg acctggtctc acacgagatg cctgacctga
     6061 tcattttgga tgtgatgatg ccaaaacttg acggtataac cacagcaacc cgcatccggc
     6121 agttctctaa cgtgcctatc atcatgctca ccgccctggg agaagaacgc gaccgtgtac
     6181 gcgggttgaa cctcggcgct gatgattacg tcgtgaagcc cttttcagct actgaactgg
     6241 tggcaagggt caaagccgtt ttacggcgca gccaattaaa agaaaacgct ggtaggtcaa
     6301 ggatattgac gcatggtgac ctgaagattg acatcgccaa ggctgaggtt ttcaaaggcg
     6361 accacccggt gtacctttct gccacggaat acaagctgtt gatccatttc gcccataacc
     6421 tggggaaggt actcagcgca gaagaattgc tcaccactac ttggggtcct ggctataaag
     6481 atgaaaaaga gatcctatgg gtgagtatcg cccggctgcg ccaaaagctg gaagacgacc
     6541 cccacgaacc tgttcacata gtcactcgca ccggtatggg ttaccagatg cccgcgcttg
     6601 atgagcaagc aaagaaatga aaaaggccag atgcgataat caagcctggt tttcatagtt
     6661 ccttgagatt ggagtagtca acatggaagt gagcccgctg gaagaacaaa gtcaactcat
     6721 cagcgcccgg ttattggccg caagggaggt gagcgaaaaa accctcgcca gagtcgctga
     6781 gcaattgggt gtttccgtga aaatggtcaa gaattacgaa agcggtcaat ctactcccag
     6841 cctgccgcaa ctggaggtat tgtctgagct gtatcgaata ccaattgaaa atcttctcag
     6901 cgggaaccca gatttactca agaagacaat cctgccgcgt gaaaaggtca acaacttcct
     6961 tgaaatcaga aatagggtca tcgccgcaac cctcaaacaa cagcgcctgg ctcaaaactg
     7021 gacaataaaa aagctggccg ctgcagtagg tatcagcgca gcgattctcc ccaggtatga
     7081 atctggcgaa acagcgattc ccgcacctat acttgaacac ctttgttctc aattaggcat
     7141 cccactgaaa tccttatatt cttcaatcac cctccaaaac agcgaaccgc cccccattgc
     7201 gtcaccaagc caggattcat ctcaattacc tgaagaagta caatccttca ttcaaaaccc
     7261 ggcaaacctg ccctaccttg aactggcgaa aaggctaagc gggatggatg ccacgaaact
     7321 gcgcgctatc gcagaaaacc tgctggagat cacattctga ccaatgactg cagaaattga
     7381 aaaacttaaa caacaggcga ttaaagacta caatcgccga aaatatgatg ccgcaattgc
     7441 cgggtttcaa tcctgccttg agttctatga aaaacagaac gatgaactgc ttgtcgcaga
     7501 aatgcgcaat aatatcagtg ttgccttgct gggaaagaac gccgcccaag aagcttatga
     7561 tatcgtcaaa ggcacagatg agacattcca aaaggtcggc gatttgaaag gccaggggat
     7621 ggcgctggcg aataccgccg ctgcccttga agcgctggat aaaaaggaag aagcactggc
     7681 tctttacgaa cacacactgg atattttcaa agaagccggc gagaaagagc tgcgaatcaa
     7741 tattctgcgc cgggtatctg atttacaact gaagaccaaa cgaggcttcc aggcccttgc
     7801 ttccatggaa gcggcatata accagagaga aaaacctacc atcaaggact ccgtgttcaa
     7861 aacaacgctg gcagccattc gcaacaaatt attcaagtaa atgctacagg caccctcgaa
     7921 tactgaaatc cgccaggctg tcagccaaga tatcacggca atcactgatt ttgtcgataa
     7981 agcttccctg actcatcgcc acctggattg gcgcccgctg gtggagtggg tttcacagcc
     8041 ccctttttta ctgcgcttcg caaatcaaca gctaaccgcc ctgttatcct gcgctccaga
     8101 tcctgaaggc gttgcctgga ttcacgcttt tatcatggac cattggtcca accaggtgaa
     8161 cgctgtctgg cactcgctgt ttgaacccgc cttgatcgaa ctggctgtaa cccgttgcca
     8221 ggttttttca ttgtccctgt ccgattggta cactaggctg ctgcgatcct ctggctttac
     8281 gccttttgag aacatcgtcg ttcttcactg gggtagagag gtacctccag tgaaaaacat
     8341 gccccctgaa gtactgattc gtccaatgga acccgctgac ctcaaccagg tagcagaagt
     8401 ggacaggcag acctttgaag ccgcctgggt catttcgcgg gcttcactgg agcgtgcgta
     8461 cctgcaatca gcgcacgcca gtgtcgctga ggtaaatgag cagataatcg gttacgagct
     8521 ctcgaccgcc agctctctct cctcccatct cgcccggctg gcagtgctgc cagaattcag
     8581 gcacctgaat atcggctatt cgcttgtcgc tcaactgttg gaatatttca cacagcgggg
     8641 aatatggcaa atcaccgtga atacccagga taataaccag gcttctttat ctctctataa
     8701 aaagctgggc tttttccaga cctctgaatc cttcccggtt ttcacgaaat ccggctgaac
     8761 aaagcaattg ttgaccggga tatttatgga caatttcagt cttatatgag ataatgcgag
     8821 gacttttatg gaggaaagat gacaaaacga gatgttgtgc aacaacgaag aaaacaaaac
     8881 cgctccaaaa attctctcac aactatttta attattgccg cctttgtttt tgtggtcgtt
     8941 ggcatggtga ttttaaccca gtacaaacca gtcggccaaa tagccacgat cacgcgaacc
     9001 accaccgctc aaaaagacgg tctatcattg ggcaacccgg atgcgccagt tcaggtgatt
     9061 gaattcgctg attttcaatg tcccgcgtgt gccaactatt ggtccgtttt agagccccaa
     9121 atcataaaag attacgtcga aacaggcaaa gcgcgtttca cctattcccc cttctccttc
     9181 cttggtcagg ggcaagcatg ggatgaatca attaaggccg ctgaagcagc ctactgcgcc
     9241 aacgaccagc agaagttttg ggaataccgc gatatggtct tcgccaatca taatggtgaa
     9301 aaccgcggag cctactcaaa agaacggctg gtggcattcg ccaagcaact tgatttggat
     9361 atgaaagcat tcaatgcctg ttttaattca ggaaaataca gtcagcaggt tataaacgat
     9421 aataagttcg ccacagaaca gggcgcaact tacaccccgt ccttcctcat taatggcaag
     9481 atagtcaacg cagctgaatt ggttcaagca atcgaaacca gtctgacaaa ttagccgaag
     9541 caaatgacca gaatgacaaa ccccggttgt aaaccggggt ttatttgaac agggatagaa
     9601 aaaatacgag tagtggggca accagaagag cgggaaggaa attccctgtg cggattttct
     9661 ttatttccat aatattgctg atcgccagtc ccgccaggat caccccacca gtagcgctga
     9721 gctcattcag catttcaggg gtcgccacac gctgtacctg ttgcgccaac agggtgatag
     9781 aaccctggta aagcaataca gggacagaag aaaaaagcac tcccgcgccc aaactggaag
     9841 caaaggctaa cgcggcaaaa gcgtccagga tggacttgat aatgagcgtg taagaacctc
     9901 cgctcaatcc atcctgtaga gagcccaaaa ccgccattgg cccgatacag aacaacagtg
     9961 ttgacgaaag aaagccctgt ataaaccggc tggagccttc tccccgcccg gaatggttta
    10021 aacgaccttc cagcttctcc cccagcgcca tcaacccctc ttcgattcgc caccattctc
    10081 ccagcaaaat cccgataagc aggcttccca gcacaatgag cgcgttttgc gtttcaagaa
    10141 aaagcgatat cgcgtacagg agtgtgaaga gacccagcgc gctgatgatg gtctgcttca
    10201 atttctcttt cagcctggat ccaagtaaaa ggcctgtcaa tcctcccacg agaatgccag
    10261 caaagttgat taatgttcca gtcaagttga cgattccctg gccatggttg gtttgtgaga
    10321 ggtctcacgc tgtatcaggt ccagttccat tgcaaaaaag agcgacgaaa gacgattaag
    10381 gtaaatcaag acatacgcgt ttgtcaggtc tccacggctg gtaagttctg caacacgccg
    10441 ttctgctctt ctggtaatgg tacgcgccag ggcgatatac gcgctggttg gtgtttctcc
    10501 gggcaatatg aatcctgcgg gtgtatccac tctcatcgaa atacggtcaa tttcagcttc
    10561 aagagagttc accgactgct cattaatttt ggcaaatttt tccctgttct ctggcgaagc
    10621 tgccacttcg gccattaatt ggtataactt gcgttggtac agaatgatct tctggcggtc
    10681 ttgcgggtcg gtgagcatgc tgcgggcaat tcccagcacc gctgatagct catccagcgt
    10741 ccccagcgcc tccatacgca aatcaaattt gtccactctc tgttcgccaa gcaaaccggt
    10801 tgagccctta tcacctgttc ctgtataaaa ttttttcatg atagttcact ttttcctgac
    10861 ataaagatag gcatagattg atttggggaa acccgtttcc agctctggtt cccaaataaa
    10921 atcaccttca ggcaacaacg cgccaagttg acgatccagt tcttgcgctt tacgttgctc
    10981 tacatatggt tctgccgcca ggcttcgaaa aagcaaatct agctttaccc gggtgctgat
    11041 attgctgcgg taacttgcgt cgattgctcc ttgctcaagg tattcatcga ccagttcgcg
    11101 atattggttc aagcgcaaag ggatctcctg acgcgcacga ttttcccacg cctgccgtcg
    11161 ggtgtcaatc agtttttcaa ccttattcat ttcatcatgc tgcaccgcaa tttcattaac
    11221 tgtttttagc cgggtaatag ccagaagcag gttaccaggc gaaagaggtt ggttgcagtg
    11281 tttcaccctc cagagaatga tgggggaaga cagatattgc gccagttctt ctccagcagc
    11341 aaggattagc cgtatgtcgt cctccatgcc ctggtacgac atttcagttc cctgtatctg
    11401 tttctgctgt atccgtggtt ttttggttgc ggctggtagt gagataccag gtggtgatcg
    11461 ttcccagccg gtcgctggta tcaaagagcg gacccatcgt cacgcccagt atctgctctg
    11521 aaaccgcgta gttgtatacc gggtaataga gcggtagagc cggtaattct tccgcgaaga
    11581 tcgcctggaa attatggtag agacgggttc gctcacccag gtcagtggtc acgcgcgcct
    11641 gctcgatcgt atcgcttgcc aggcgattgt tccactggga gtaattctgc ccattttccg
    11701 cctgccccaa gtcccaaaac gggtaggggt cagggtcagg cgtgcggctt aagttcaagt
    11761 cgaccagagc tgcctggtaa gacctcggtt ctagtttttc agcaacgaac acatctgggg
    11821 gtacgccctc cagtgtaacg ttcaccccca aggcttgcca gttgttggcg attaactgtg
    11881 ctattccaag gtgcaggtca tcatctggat aagacagcac aaaagatagc ggaacctctt
    11941 cttttgccct ggtggtattg ttttcaccag cgatcacgta acccgcgttc ttgagggttt
    12001 cggctgccaa ttgcgggtca taaccgattt cagcaatact ctcgttgtaa gcccaagttc
    12061 cagggaggat aacgctattc gccacaatcg cctgcccgtt gaacatctgc gagatgatcg
    12121 cctggcgatt caaacccagc atcaacgctt tgcgcacctc tatctccttg aaaaacggga
    12181 cgcctgggtc atttagattc aataacacca agctgatgcg cggtaagcgt cctgtataaa
    12241 tacgaagatc ctcgttcgca agcgcttgcg gcaacaggtc tgtgggaact tcactgatgc
    12301 cctgcacggc accctgaacg tatgcctgat aggcgcttga tgcgtcagga taataacgga
    12361 aaacgatctc ttgcaaatag ggtcgtccac cggcatagtc ttcatttgcc agaagtcgaa
    12421 tgccgctcac tgttgacccc tccatcagca gttcaccaaa gcggtacggg ccgctgccta
    12481 ccggttgaag gttgatggcg gatgcggcaa tttcgttgat atccatgcca gcaaaaaggt
    12541 gctgtggcaa cacgccaaag gtcagatagt ccataaaagg cgcgtaggct tccggaagtt
    12601 ttaattgcat atgctggtca tcgaagagat acacttctat cgaatcccat aatttgcgta
    12661 tatcttccgg aatatagaca cctcctgttc tgagcaattc gatggtgaac aaaacatctt
    12721 tggtggtcag cttctcgccg tcatgccaaa ccagccctgg ttttagcgag atgttataca
    12781 gtatcccatc ctgagaaatc ccgatcgttt cggccaggtc aggagcggga atacctcttg
    12841 agtcaaattt caccaggcca gagaaaatca gtttatctac atctctatcc acactattgg
    12901 tactatccag cagcgggttc atgcgctggg ggtatccaat cagggcctca gtgtaaaccc
    12961 ctccttgcgc cggttgtgga gtgttcactt ctcccagccc gccacgcttt tctaaaagca
    13021 agagagttcc aactaccaaa ccagtcagaa agagaacgag taattgccag cgatattttt
    13081 tcataaggtt tatagaaaat ggcgactacc ctgtttcaag gttagtcgcc tcgaatgatt
    13141 ggtttcaggc tgttccttga atgacccggt ctacccctgg ataaggaccg cggcaaaggt
    13201 aagaataaaa aataaaccac tgagtacgat cgtcacccaa aacagcgttt tttcgattcc
    13261 cctgcgggca gtgaagacac cgccagaatc ctgaccggtc aatcctccaa gccctacccc
    13321 tttattttgc aggattacgc tcgcgataag agctatcgaa gttataatta gcgcgatgtc
    13381 aatgtaaagt tccaccagaa acctcctgaa atttcaaaca ccgcagatta taccgttcat
    13441 aaccgaaaat cgtcaaccta atcgtgttag ttcacctaaa atgttactaa gataacatcg
    13501 ttaggccaaa agaa
//
LOCUS       DAOSPM010000050        12611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_4980_length_12611_cov_12.480408, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000050 DAOSPM010000000
VERSION     DAOSPM010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12611
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4980_length_12611_cov_12.480408"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLW25_05775"
     CDS             <1..477
                     /locus_tag="PLW25_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54031.1"
                     /translation="RGRYELDSTVNQPIYVVPSEVQRPRLVCQTVIQDAMVLNVGMFA
                     LDDSPAAPAAAPAEPVATEEAPAPTGPAYPGSITIVVSPQDTLILNYLALAGAKLSLA
                     LKSAGDGAQFSTDPVTLQYIMDQKNIPAPVKLPYGVEPRVDTLLYPAFDNYVLNQP"
     gene            523..1755
                     /locus_tag="PLW25_05780"
     CDS             523..1755
                     /locus_tag="PLW25_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS54032.1"
                     /translation="MAEQEKITVVIVDDIDESREMILRMLQFEPAIEVIGSARSGRQA
                     LELAEQLKPDVMVMDINMPDMDGITATEAIRKKVPFIQVVILSVQNDPNYMRRAMLAG
                     ARDFLSKPPLIDELTTAVRRAGDLALEEKSKFVVAPFNINGAPGAIPLGMVLPNPPSG
                     KIIVVYSPKGGVGTTTVATNLATLLKTEQNKVALVDANLLFGDVAVFFNEHARNSFLE
                     LLEHSHELDPEIIEDVMAVNQKTGIHIMTAPKQPEFGDGGKGQSISRILTYMRQLYNF
                     IVVDSSTYLTEVVQTCLDIADLIILVTTQDIPAIKNAHQFLSLADASGIERERILFVM
                     NRYDRRVAITPEKVGATLKQPVRVSIPFEDRIISNAVNRGVPFILENKNLLASKAMVT
                     LADQVKESVTLVKEEIAS"
     gene            1773..3167
                     /locus_tag="PLW25_05785"
     CDS             1773..3167
                     /locus_tag="PLW25_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOS54033.1"
                     /translation="MSILRRIQSTDSQNTSAQNGVADSSSTPPLQARRVLPSTVQASQ
                     DTYQDLKTRVQNKLLSELDPSVDVSRVHEVRRTIQDLYEQILAEENFVLSRAEKSRLF
                     EQIAAEILGLGPLQSLLEDDTITEIMVNGARNIYIERRGKISKVPLTFENDEHVMRII
                     ERIVSPLGRRIDESSPYVDARLPDGSRVNAIIPPISLVGPVLTIRKFARIPLSVDQLV
                     QLGSITPEAIQFLDACVKARMNIVISGGTGSGKTTLLNVLSAFIPSDERIITIENAAE
                     LQLRQEHVVTLESRPPNIENRGEITIRNLVINALRMRPDRIIVGEIRDEAALDMLQAM
                     NTGHDGSMTTCHSNTPRDSLARIETMTMMAGMDLPVRAIREQVSSAIDLVVHQERLRD
                     GSRKVVNITEVSGMEGDIITMTDIFVFEQLGFENNRVIGKLRPTGLRPKFIEKIEASG
                     IHLPPAVFGIGVRR"
     gene            3171..4148
                     /locus_tag="PLW25_05790"
     CDS             3171..4148
                     /locus_tag="PLW25_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOS54034.1"
                     /translation="MNPVIFLWIAGVLVAGVIGLSVYLTISGQRSAEEARLDAFAERG
                     ISVDENLSTKGGSVVSEWLSRKVAKTSWADKVSKELVQADLKMKPGEYLLILIVSVLV
                     VAAFGFFIGSKSFLLALIGGFVGFLLPGIYIRSQKNRRLIKFNNQLGDMLSLMVNGLR
                     AGFSTLQAMESVAKEMPPPISEEFRRVVQEVSLGVSTEKALDNLLRRIPSEDLDLVIT
                     AIKVQREVGGNLAEILDTISHTIRERVRIKGEIRVLTAQMKFSAQFLALLPAFVFVAI
                     LVINREYLLILIDPEANTPLPCGFIAIAVAILLSIIGYFVMNRFTKIEV"
     gene            4161..5105
                     /locus_tag="PLW25_05795"
     CDS             4161..5105
                     /locus_tag="PLW25_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOS54035.1"
                     /translation="MWVILALLGVILAIAVVLVVIGIRNPYSENDMRLFERLGDFSKS
                     GEQVDLEKLELSLPFTERVIYPLARKLGEITLRFTPQNWISRINRRLELAGRSSKVDA
                     TVFLTLQFIFGLLIGGFTFTLLKLLGNNIPAGRVFLFTLVMTLLGFYLPHFQLSSSIT
                     NRQKQVRKDMPDALDLLTICVEAGLGFDAAMARVAEKWNSPLSMAFARVIQEVQLGKL
                     RREALHDMQDNIGISELTSFVAAIIQSEQLGVSMSKVLRVQADQMRVRRRQLAEEEAH
                     KAPIKMLIPMAFLMFPSLLIILLAPAVLQIIKSGIFGG"
     gene            5130..5876
                     /locus_tag="PLW25_05800"
     CDS             5130..5876
                     /locus_tag="PLW25_05800"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOS54036.1"
                     /translation="MNILFALHRLFWAAADWLYPPHCAGCGHHGERFCASCLAQVVLI
                     TDARCAFCGDKTSDGSSVCMKCNRNSVSFNAAASWAVYGGELRKAIHALKYRQDMALG
                     AFFATFLIATIEKQGWNFDLVIPIPLSPDRMKERGYNQSELLSRPIAFYFQVPHSSMA
                     LIRIKDTGTQVNHTKIERDLMLKDAFYANPDKLNGRKVLLVDDIITTGSTINHCAKAL
                     KEAGTSEVYALSIAKTLKKDHRCSNETNKS"
     gene            5894..6454
                     /gene="raiA"
                     /locus_tag="PLW25_05805"
     CDS             5894..6454
                     /gene="raiA"
                     /locus_tag="PLW25_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOS54037.1"
                     /translation="MDEKIVITVKNMELTDRIKEYVEKKTPRLERLLPVIESMKFDLS
                     YAKNARNRSDREIAQITITGKGFVLRTEERDVDLLAAIDKAADKMQRQIERFKGKRTR
                     GRGDGTPASEVIPLPVENEPDRKTAIVRRKTFTLVPMDENEAIEQMALLDHEDFFIFY
                     NVNTNSINVLYQRRDGNYGLIEPKIG"
     gene            6681..7664
                     /locus_tag="PLW25_05810"
     CDS             6681..7664
                     /locus_tag="PLW25_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS54038.1"
                     /translation="MNLVTGATGHIGNVLVRELLNRGESVRVLVLPDEDLTPIKGLNV
                     EIHYGNILDPQCLVNAVTGIDTVFHLAGIISISSSHVEIVHKVNVEGTRNMVDAAMKA
                     GVRRFIYTSSIHAFKRVPHGMVIDETTPIDPDFSVGAYDRSKAEATLLVQEKVKEGLP
                     AIIVCPTGVLGPYDYKGSELGELMNSWMLKKVNYMINGSYDFVDVRDVVQGMIQARDK
                     GTIGQLYILSGELIRVSDLWHIVKELFVIKSRLVNIPLRLARFFANFSELYYKITRTK
                     PKFTQYSIETLQSNAVIDNAKARQSLGYSPRTIRDTIRDTINWWKQHPVKR"
     gene            7673..8482
                     /locus_tag="PLW25_05815"
     CDS             7673..8482
                     /locus_tag="PLW25_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS54039.1"
                     /translation="MPPSQKKPRIALITGASSGIGADVALLFAKNGVHVILTARRLER
                     LREIEAKIASAGGKATIIQADLSQEDERLRLLDELNQSQLFPDVLVNNAGLAWYGYFH
                     EMPWTISRDIIDLNIAASTHMALLFLPAMVRNKYGFIINIGSIAGKLNEQGIAIYSAS
                     KAYLDAFTTCTHRELKHSGVHISVVRAGPVKTEFFDTARKLPNGGAIPAERFAIPSAR
                     VAKAVWSAVKFPRKVIYVPFYMFLSPLLEVLFSSLIDLAGPLLLKRMKKPH"
     gene            8585..9142
                     /gene="efp"
                     /locus_tag="PLW25_05820"
     CDS             8585..9142
                     /gene="efp"
                     /locus_tag="PLW25_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOS54040.1"
                     /translation="MIDVNDLRKGVIFELDGNLYRVLEYSFNKPGRGNATIRIKARDL
                     RKGNTLEKTFTSGERIKDVRLDFHNVQYLYNDGEIYHFMDLETYEQPAISGSVIGELA
                     GFLKPEMEVKLTFFENEPIDIELPTTVDLTVTFAEPAVKGDTATGVTKKVVTETGLEV
                     GVPYFVQTGDTIRVDTRTGEYVTRV"
     gene            9197..9538
                     /locus_tag="PLW25_05825"
     CDS             9197..9538
                     /locus_tag="PLW25_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54041.1"
                     /translation="MRTPAGTECPFFYGDYYRGRNIEECRLIGNQPPPNNWTADLCRS
                     CPVPSIKRANACENMQLSAVIKRRFRLFKRYVLVTAYCHLAKEIVQQPHIGCGQCHRL
                     EFVESAKDDFS"
     gene            9698..11560
                     /gene="ftsH"
                     /locus_tag="PLW25_05830"
     CDS             9698..11560
                     /gene="ftsH"
                     /locus_tag="PLW25_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOS54042.1"
                     /translation="MKPRSPSYIVYLLLFASIIVLVVFSLNRGNTADSVLTINQLADE
                     VKKGSVAKIVEEKNDLKIIMTSGAEKTSTKETETTLVEQLLALGVTPEELNPQKGIKI
                     EIKPPGAFAGILNALFYILPFLLLGGAFFFIFRQAQGSNNAAMSFGKSRARMFTGDHP
                     TVTFADVAGIEESLEELKEVVEFLREPQKFVALGARIPKGVLLVGPPGTGKTLLAKAV
                     SGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKKNSPCIVFVDEIDAVGRQRGAG
                     LGGSHDEREQTLNQMLVEMDGFDTDTNIIIMAATNRPDILDPALLRPGRFDRRVVLDR
                     PDVRGREAILRVHIKGKPVDPEVDLSLLGKATPGFVGADIENLVNEAAILAARRNKKS
                     IGQSEFEESIERVIAGPERKSRLISQEEKRIVAFHEAGHAVVMNALPEADPVQKVSII
                     ARGMAAGYTIALPMDDRTLMPRKKLIAEMVGLLGGRAAEELVFDDITSGASNDIERVT
                     QLARNMVTRLGMSAELGPMVYGQKEELIFLGREISEQRDYSESVAEKIDKEVRSLVNN
                     AYSQAKSILTTYRKELDDIANRLLEVETLSREEFEKIFPPPVPHHSGTPEITLN"
     gene            complement(11584..>12611)
                     /locus_tag="PLW25_05835"
     CDS             complement(11584..>12611)
                     /locus_tag="PLW25_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS54043.1"
                     /translation="PKAAIGTHRNLVANVTQFKNWLVNLDDGKETYLVAIPLYHVYGL
                     VLGLLLGIATGATLVLLQKPGDVDEMLHAITKYPVSYFPAVPSIFNLINNHPGVKRGD
                     YQLNRIKACISGSAPLMEAVRENFEKLTGASLVEGYGLSEAPTATHCNPILGEKRAGS
                     IGLPLPDVDCRIVSLANNQADMPIGEEGELLIRGPQVMPGYFNHQEESSALLESGWLR
                     TGDIARMDAEGYFYLSGRIKDLIKVHGMQVWPTEVEQVMSEHPAIKECAVAGVPDEKS
                     GERVKAWLVLQEGHGMALDNLRQHCQGKLAAYKIPVEISICASLPKTPVGKVLRRELV
                     REHMERK"
BASE COUNT         3176 a         3159 c         3068 g         3208 t
ORIGIN      
        1 cgcgggcgtt atgaactgga cagcaccgtg aaccaaccca tttacgttgt cccctcagaa
       61 gttcaacgtc ccaggctggt ctgccagacg gtcatccagg acgccatggt gttgaacgtg
      121 ggcatgttcg ctttggatga ttcaccggcg gcaccggccg cagcgcccgc tgagccagtg
      181 gcaacagaag aagcgcctgc gcccacaggc ccggcttacc cgggctcgat caccatcgtt
      241 gtttctccgc aggatacgct catcctgaat tatctcgcgc tcgccggggc caagctctca
      301 cttgcgctga agagcgccgg ggatggcgcg cagttctcca ctgatccggt taccctgcag
      361 tacatcatgg atcaaaagaa cattcccgcg ccggtcaagc tgccctatgg tgttgaaccg
      421 agggtggata cgctgctcta ccccgctttt gataattacg ttctgaatca gccttgacaa
      481 aagcgaaatc tgattctgca ccagcatgat cctggagaga atatggccga gcaggaaaaa
      541 atcactgttg taatagtaga cgacatcgac gaatctcgtg agatgatcct gcgcatgctg
      601 caattcgagc ctgcgatcga agtgatcggg tctgcccgtt ctggcaggca ggcgcttgaa
      661 ttggcggagc agttaaagcc ggatgtgatg gtcatggata ttaacatgcc ggatatggac
      721 ggtatcaccg ccacagaggc catccgcaag aaggtaccgt tcatccaggt ggtcatcctt
      781 tcggtgcaaa atgaccccaa ttacatgcgc cgcgccatgc tggcaggcgc gagggatttt
      841 ctttcaaaac cacctttgat cgatgaatta accacagccg tcaggcgcgc gggagattta
      901 gcccttgagg agaaatccaa gtttgtggtg gcgcccttta atatcaacgg cgctccaggc
      961 gccataccgc tgggcatggt tctacccaac ccgccatcgg gaaaaatcat cgtcgtttac
     1021 agccccaaag gcggcgtcgg caccacaact gtggccacca acctcgcaac ccttctcaaa
     1081 acagagcaga acaaggtggc gctggtggat gccaatctcc tttttggcga cgtggcggtt
     1141 ttcttcaacg aacatgcccg taattctttc cttgaactgc ttgaacattc acacgaactt
     1201 gaccctgaga tcattgaaga tgtcatggcg gttaaccaga aaacgggcat ccatattatg
     1261 actgccccca agcagccaga atttggggat ggcggaaaag gtcaatccat ttcacgcatt
     1321 ctgacctata tgcgccagtt gtacaacttt atcgttgtcg attcctccac ttatcttaca
     1381 gaagtcgttc agacctgcct cgacatcgct gatttgatca ttcttgtaac cactcaagat
     1441 attcctgcaa tcaagaatgc ccaccagttt ctttctttgg cggatgcttc gggaattgaa
     1501 cgcgagcgga tcctctttgt gatgaatcgc tatgaccgcc gcgtggccat cacccctgaa
     1561 aaggtcggtg caacccttaa acaacctgtc agggtgagta tcccctttga agatcgcatt
     1621 atcagcaacg cggtgaatcg cggtgttcca tttattctcg aaaacaagaa ccttctcgcg
     1681 tcaaaagcca tggtcaccct ggcagaccag gtaaaagaaa gcgtgaccct tgtcaaagag
     1741 gaaatcgctt cctgatttga ggagctagat caatgtccat tttaaggcgc atccaatcaa
     1801 ctgacagcca aaacacctcg gctcaaaatg gcgtcgccga ttcttcttct accccgccct
     1861 tacaggcgag gcgcgttcta ccctctaccg ttcaagccag ccaggatacc taccaggact
     1921 taaaaacccg cgttcagaac aaattgctgt ctgaattgga tcccagtgtg gatgtttcca
     1981 gagtccacga ggtgcgccgg acgatacaag atttgtatga gcagatacta gcggaagaga
     2041 acttcgttct ttcccgtgca gagaaaagtc gcttatttga acaaatcgcg gcagagatcc
     2101 tcggtctggg accgctgcag tccttactgg aggatgacac catcactgaa attatggtca
     2161 acggcgcgcg aaatatctac atcgagcggc gcggtaagat ttcaaaggtc cctcttactt
     2221 ttgaaaatga tgaacatgtg atgcgcatca tcgaaaggat cgtgtctccg ctcggcagac
     2281 gcattgacga atccagccct tacgtagacg cgcgtctgcc ggatggctcg cgtgtgaacg
     2341 ccatcatccc gccaatttca ctggtgggcc cagtattgac cattcgaaaa ttcgcccgca
     2401 tcccgctttc agttgatcaa cttgttcagt tgggctccat cacgcctgaa gccattcagt
     2461 ttttggatgc ctgtgtgaaa gcacgcatga atattgttat ctccggcggt accggctctg
     2521 ggaagacgac cctgctcaac gtcctttccg ccttcattcc ctcggatgag cgtattatca
     2581 ccatcgaaaa cgccgccgag ctccagctgc gtcaggaaca cgtggttacg cttgaatcac
     2641 gaccgccgaa tattgaaaac aggggcgaaa tcaccatccg caacctggtc attaacgcat
     2701 tgcgcatgcg ccctgaccgc attatcgtcg gcgagatccg cgatgaagcc gcgcttgaca
     2761 tgctgcaggc gatgaatacc ggtcatgacg gttcaatgac cacctgccat tccaacacac
     2821 cgcgcgatag cctggcgaga attgaaacca tgaccatgat ggcgggcatg gacctgcccg
     2881 tgcgcgccat ccgggaacag gtgagctctg ccatcgacct ggtagttcat caagagcgcc
     2941 tgcgagatgg ctcgcgtaaa gtggtgaata tcaccgaagt ttctggtatg gaaggcgata
     3001 tcatcacgat gacggatatt tttgtatttg aacaacttgg atttgaaaat aaccgcgtga
     3061 ttggcaagct tcgccccact ggtctgcgcc ctaagttcat tgaaaaaatc gaagcatccg
     3121 gaattcacct gccaccggcg gtttttggaa ttggcgtgag aaggtaaagc atgaatccag
     3181 tgatcttttt atggatagca ggcgtgttgg ttgccggtgt gattggtctg agcgtgtatc
     3241 tgaccatctc cgggcagcgc tctgccgaag aagccagact ggatgcgttt gcggaacgtg
     3301 gcatctcagt tgatgaaaac ctcagcacca agggaggctc cgtcgtctct gaatggctgt
     3361 cacgtaaagt ggcaaaaacc tcctgggcgg ataaggtatc aaaggagctt gtgcaggcag
     3421 acctcaagat gaagcctggc gaatacctgc tcattcttat cgtttcagtg ctggttgtgg
     3481 cagcctttgg cttctttata ggatcaaagt cattcttgct cgcccttatt ggcggattcg
     3541 tgggttttct gttgcccggt atctacatcc gttcacaaaa aaaccgccgg ttgatcaagt
     3601 ttaacaacca gttgggtgat atgctcagcc tgatggttaa cggtcttagg gctgggtttt
     3661 ccactttgca agccatggaa tcagtcgcca aagaaatgcc cccgcctatc agcgaagagt
     3721 ttcgccgtgt tgttcaagag gtctctcttg gcgtgtcaac agaaaaagcg ctggacaact
     3781 tgttgcgaag aattcctagt gaagaccttg atctggtgat caccgccatc aaagtgcagc
     3841 gagaggtagg cggtaatctc gctgaaatcc tggataccat ctcgcatacc atccgcgaac
     3901 gcgtgcgcat caagggtgaa attcgcgtgt tgaccgctca gatgaagttt tctgctcaat
     3961 ttttggcttt gctgcctgcg tttgtctttg tggccatcct ggtaattaac cgcgaatacc
     4021 tgttgattct gattgaccct gaagctaata cacctttacc ttgtggtttt atcgcaatcg
     4081 cggttgcgat attgctatcg attatcggat acttcgtcat gaatcgtttc acgaagatcg
     4141 aagtttaaga ggaacctgat atgtgggtga tactggcttt gctgggcgtc attttagcca
     4201 tcgcggttgt gttggtggtg atcggaatcc gcaatccgta ttctgaaaat gatatgaggt
     4261 tgtttgaacg cctgggagat ttcagtaaaa gcggtgagca agttgacctt gaaaaattgg
     4321 aactctcatt gccttttaca gaacgcgtca tctacccgct ggcgcgcaaa cttggcgaga
     4381 ttaccctgcg cttcacaccg caaaactgga tcagccgtat caaccgaagg ttggagctgg
     4441 caggcaggtc ctccaaagtc gacgccaccg ttttcctcac cctgcaattc atctttggcc
     4501 tcctcattgg cggtttcact ttcacgctat taaaactgct ggggaataat attcccgccg
     4561 gcagggtctt tctttttacg ctggtgatga cgctgctcgg cttttactta cctcatttcc
     4621 agttatcaag ctccatcacg aaccggcaaa aacaggtgcg caaggacatg ccggacgcgc
     4681 ttgacctgtt aacgatttgc gtggaagcag ggctgggctt tgatgcggcg atggcccgcg
     4741 tggctgaaaa atggaactca cccctctcaa tggcgttcgc ccgcgtcatc caggaggttc
     4801 agttgggcaa attgcgccgt gaggctctgc acgacatgca agataatatc ggtatcagtg
     4861 agttgaccag tttcgtggcg gctatcattc aaagtgaaca gctcggcgtc agtatgtcca
     4921 aggtgctgcg cgtgcaggca gatcagatgc gcgtaaggcg acgccagctg gctgaagaag
     4981 aagcccataa ggctccaatt aaaatgttaa tcccgatggc gttcctcatg ttcccctcgc
     5041 tcttgatcat cctgcttgcc ccggcggtgc tgcaaatcat caagtcaggt atatttggcg
     5101 gctgagcggt ttttctgtaa aatgaacatg tgaatatttt attcgccctg caccgtcttt
     5161 tctgggctgc tgcggactgg ctctaccctc cgcactgcgc tggttgcggg catcacggag
     5221 aacggttctg tgccagttgc ctcgctcaag tggtcctgat tactgatgcc cgctgcgcct
     5281 tttgtgggga taaaacgagt gacggttctt ccgtatgcat gaaatgtaac agaaacagtg
     5341 tatcttttaa tgctgccgct tcctgggcgg tgtatggagg agagctgcga aaagcgattc
     5401 atgcgctcaa ataccggcag gatatggcac tcggggcttt ttttgcgacc ttccttatcg
     5461 ccacaataga aaaacagggt tggaatttcg accttgtaat tcctattcct ctttctccag
     5521 accggatgaa agaacgaggt tacaaccaat cagaattgct ttcgagaccc atcgctttct
     5581 atttccaggt tccacattca tcgatggcat taataagaat taaagacacc gggactcagg
     5641 tgaaccatac aaaaattgag cgtgatttaa tgctgaaaga tgcattttac gcaaatcctg
     5701 ataaacttaa tggaagaaaa gtgctgctgg tggatgatat tatcactacc ggctcaacga
     5761 tcaatcattg tgcaaaagcg cttaaagaag ctggcacctc tgaagtgtat gccctttcaa
     5821 ttgcaaaaac cttgaaaaaa gaccaccggt gcagcaatga aacgaacaaa tcttaattct
     5881 tttggaggta aagatggacg aaaaaattgt gatcacggta aagaacatgg agttgacaga
     5941 ccgaatcaaa gagtacgttg agaagaaaac cccacgttta gaaagactct tgcccgttat
     6001 tgaatccatg aaatttgacc tctcctatgc aaaaaacgcc cgaaaccgct ctgaccggga
     6061 gatcgctcaa atcaccatta ccggaaaagg attcgtgctc cgcaccgagg aacgcgatgt
     6121 ggacctgtta gcagccatcg acaaggcggc tgacaaaatg cagcggcaga tagagcgttt
     6181 caaggggaaa cgcacccgag gacggggtga tggtacgcct gcttctgagg taatcccact
     6241 gccggtggaa aatgagccgg atcgcaagac cgccattgtg cgcagaaaaa ctttcaccct
     6301 ggtgccaatg gacgaaaacg aagcgatcga acagatggcg ctccttgacc atgaagattt
     6361 ctttattttc tacaatgtca acaccaattc gatcaatgtg ctgtatcagc gccgggatgg
     6421 taattacggg ttaattgaac cgaagatagg ctaaatacga tctctttttt tcacagggcc
     6481 gttgaaagcg gccctgtttt tatttaaatg tgacctgtta tctaacttac gtcacatcca
     6541 taagttgggt tgtgtgaata aaatggaaga aaatcattgc gactggtttt gaaaacaaag
     6601 acatgtcaac gctgcttatt gctgtattcg cttgtaacat gcctgaaatc aaagctgagt
     6661 tgtatttttg gaggttataa atgaatttag ttactggcgc gactggtcac ataggcaatg
     6721 ttctggtgag agaacttctc aatcgcggcg aatcagtacg cgttctggtt ctccctgatg
     6781 aagacctgac gcccatcaag ggtctcaatg tagaaattca ttacggcaac attttagacc
     6841 ctcaatgcct ggtaaatgcc gtgacaggaa ttgatactgt attccatctt gcagggatca
     6901 tttcaatctc ttcaagtcat gttgagatcg tgcataaagt aaatgtcgaa ggtacccgta
     6961 acatggttga cgccgccatg aaggccggcg tgcgcaggtt tatttacacc agttctattc
     7021 acgcctttaa acgcgtgccg catgggatgg tcattgatga aacaaccccg atcgaccccg
     7081 acttcagcgt cggcgcttac gacagatcaa aagccgaagc cacacttctg gtgcaagaga
     7141 aagtcaagga aggattgcct gcaatcatcg tttgtcctac gggtgtgctc ggcccttacg
     7201 actacaaagg atctgaactt ggcgagttga tgaacagctg gatgttgaag aaggtcaatt
     7261 atatgatcaa cggcagctac gatttcgtcg acgtccgcga tgtcgtgcaa ggcatgatcc
     7321 aggctcggga taaagggacg attggacaac tctatatcct ttccggcgag ctcatccggg
     7381 tttctgatct gtggcacatc gtaaaagagt tatttgtcat caaatcacga ttggttaata
     7441 tcccccttcg attggcgcga ttttttgcca acttttctga gctgtactat aaaattaccc
     7501 gcaccaagcc gaaatttacc cagtacagca tcgaaacatt gcaaagcaac gcggttatcg
     7561 acaatgcgaa agcccgccaa tcacttggtt attctccgcg aaccatcaga gataccattc
     7621 gcgatacgat caattggtgg aagcagcacc cagtcaagcg ttgacccaac ttatgccacc
     7681 gagccagaag aaacccagaa tcgccttgat cacgggtgct tcaagcggca taggcgctga
     7741 cgttgccctg ctttttgcca aaaatggggt gcacgtaatc ctgaccgcac gccgccttga
     7801 aagattgcgg gagattgaag caaaaatcgc ttctgcaggc ggcaaagcca caatcatcca
     7861 ggcagacctg tcacaggaag atgagcgcct gcgtttgctg gatgaactaa atcaatccca
     7921 attattccct gatgttctag tgaacaatgc cgggctggcc tggtacggct acttccacga
     7981 gatgccctgg acaatctcca gagacatcat cgacctgaat atcgctgctt caacacacat
     8041 ggcactgctt ttccttcctg ccatggtcag gaataaatat ggatttatta tcaatatcgg
     8101 ctccatcgcc ggaaaactga atgagcaggg catcgctatt tattccgctt ctaaagccta
     8161 cctggatgca tttaccactt gcactcatcg cgaattaaaa cactctggcg tgcacatttc
     8221 agtcgtccgc gccggtccgg taaaaacgga gttctttgac accgctcgta agctgccaaa
     8281 cggaggagct attcccgccg agaggtttgc cattccttca gcgcgtgtgg caaaagcagt
     8341 atggagcgcc gtgaagtttc cgcgcaaagt catttatgtg cctttttata tgtttctatc
     8401 cccgctgctc gaagtcttgt tttcttcact catcgacctg gcaggtccac ttttgctcaa
     8461 acgcatgaaa aaacctcact aaaattttta atttatcttt aatctcactg attttccttg
     8521 tcattgatca ttggctgggc tataatctca aggttatatc cgaaattgaa aagagggctt
     8581 gttaatgatc gatgttaatg atctacgaaa aggtgtgatt tttgaactcg atggaaattt
     8641 ataccgggtg ttggaatact catttaataa acccggacgc ggcaacgcca caatccgtat
     8701 caaagcccgt gacctgagaa aaggaaacac cctggagaaa accttcacct ccggagagcg
     8761 cattaaagac gtgagattgg actttcacaa tgttcagtat ctgtataacg atggtgaaat
     8821 ttaccacttc atggacctgg aaacctacga acagcctgcc atttcagggt ctgtaattgg
     8881 ggagctcgct gggtttctaa aacctgaaat ggaagtgaaa ttaactttct ttgaaaatga
     8941 acccatcgac atcgaacttc ccaccactgt cgaccttacg gttactttcg ctgagcctgc
     9001 cgtcaaaggg gatacggcta ccggggtgac gaaaaaggta gtcactgaaa caggtttgga
     9061 agtcggcgtg ccgtattttg ttcaaacggg tgatacgatc cgcgtagata cccgcaccgg
     9121 cgaatacgtt accagagtat aaggcttctt ttcagccccg tttaagcctg tgaagggcaa
     9181 gttcatttga gagactatgc gcacccctgc cggaacagaa tgcccgtttt tttatggtga
     9241 ctattaccga gggagaaata tcgaagaatg ccgtttgatt ggcaatcaac ctccccctaa
     9301 taattggact gctgatctct gccgttcctg ccctgttcct tccatcaaac gcgctaatgc
     9361 ttgtgaaaat atgcaattat ccgcggttat taaaagacgg tttcgattat tcaaacgcta
     9421 tgtactggta acagcctatt gccaccttgc gaaagagata gttcaacaac cgcatattgg
     9481 ttgcggtcaa tgccaccgtt tggaattcgt agaatccgcc aaggatgatt tttcttaacc
     9541 aacactgggt tgccgaaaga gtggcattaa cttactcacg cttgaaatta cttattagaa
     9601 atctattaat ctttcccttt ttgtcttccc cacaccgaat agttgatcaa gggttggtat
     9661 aatcgttgta gtttattcgc atcttttgga ggagcttgtg aaacctagga gtccatcgta
     9721 tatcgtctat ctactcctct ttgcctcaat catcgtattg gtggtgtttt ctttgaatcg
     9781 cggaaacacc gcagatagtg ttctcactat caaccagctg gctgatgaag tgaaaaaagg
     9841 cagcgtcgca aaaattgttg aagagaaaaa tgatttaaaa atcatcatga cctccggcgc
     9901 tgaaaaaact tccaccaagg aaactgagac caccttagtg gaacaattgc ttgcccttgg
     9961 tgtcacacct gaagagctca atccccaaaa agggatcaag atagagatca aaccgcccgg
    10021 agcattcgcc ggtattctaa atgcattgtt ttacattctg cccttcctgt tattaggcgg
    10081 tgcttttttc tttatcttcc ggcaggcgca agggagtaat aacgctgcca tgtcgttcgg
    10141 taaatcacgg gcgcgtatgt ttaccggcga ccaccccacg gtgacttttg cagatgtggc
    10201 aggcatcgag gagtctctgg aggagttgaa agaagtagtc gaatttttac gggaaccaca
    10261 gaaatttgtc gctttgggtg cgcgtattcc caaaggcgtt ttactggtcg gtcctccagg
    10321 gacaggcaag actcttctag ccaaagccgt ttccggtgaa gccggggtgc ccttcttctc
    10381 catctctggc tccgaattcg ttgaaatgtt cgtcggtgtg ggtgccagcc gcgtaagaga
    10441 tcttttcgac caggcaaaaa agaattctcc ttgcattgtg tttgtagatg aaattgatgc
    10501 tgttggtcgt caacgcggcg ctggcctggg tggctcgcac gatgagcgcg aacaaacgct
    10561 aaaccagatg ctggtcgaaa tggatggatt cgatactgac actaacatca tcatcatggc
    10621 tgctaccaac cgacccgaca ttctggaccc cgcgttactt cgccctggcc gttttgaccg
    10681 gcgtgtggtg cttgaccgac ccgacgtacg aggtcgcgaa gcgattcttc gcgttcatat
    10741 taaaggcaaa ccagttgacc cagaggtgga cctttcacta ttaggaaaag caacacctgg
    10801 atttgtgggt gccgatattg aaaacctggt aaacgaggca gccattttag ccgcacgccg
    10861 taataagaaa agtattgggc agagcgagtt tgaggaatcg attgaacggg tcatcgcagg
    10921 gcctgaacgt aagagccgcc tgatcagcca agaagaaaaa cgtattgtcg ccttccacga
    10981 agctggtcac gccgtggtaa tgaatgctct gcctgaggcc gacccggtac aaaaagtttc
    11041 catcatcgca cgcggcatgg cagccggcta tactatcgcg ttacccatgg acgaccgtac
    11101 cttgatgccc cgtaagaaat tgatcgctga aatggttggc ctgcttggtg gtcgtgcagc
    11161 ggaagaactc gtttttgacg atatcacttc tggtgcctcg aatgatattg aacgggttac
    11221 ccagctcgcc cgtaacatgg ttacgcgatt gggaatgagc gctgagctag gccccatggt
    11281 ctacggacag aaagaggaat tgatcttcct cggcagagag atctcagaac agcgtgatta
    11341 ctctgaatct gtagccgaaa agatcgataa ggaagtgcgt tcattggtca ataacgccta
    11401 ttcgcaggca aaatcgattc taaccactta ccgtaaagag ctcgatgaca tcgccaatcg
    11461 tctgcttgaa gtggaaacgc tctcgcgaga agagtttgaa aaaatcttcc cgccacccgt
    11521 tccacatcat tccggaactc cggagataac cctcaattga taaaagggag cgtctgctct
    11581 cttttatttt cgctccatgt gttctctgac cagttctcgc ctcagcactt ttccaactgg
    11641 cgttttgggt aatgaagcac aaatggatat ttcaacaggt atcttataag ctgccagctt
    11701 tccctggcaa tgttgccgca ggttatcaag cgccattcca tggccttctt gtaaaaccaa
    11761 ccaggctttc acgcgttccc cgcttttttc atctggcact ccggcaacag cgcattcttt
    11821 aattgctggg tgttcactca ttacctgttc aacttctgtc ggccaaactt gcataccgtg
    11881 tactttaatc agatccttga ttctccctga aagataaaaa tacccctcag cgtccattct
    11941 ggcaatgtca ccggtgcgca gccaaccact ctcgagcaat gcgctgcttt cttcttggtg
    12001 attgaaatat cctggcatca cctgcgggcc gcgaattaaa agctcgcctt cttctccgat
    12061 gggcatgtcc gcctggttgt ttgccaggct tacaatccgg caatccacat caggcaaggg
    12121 tagtccaatt gaaccagcgc gcttttcgcc caaaatagga ttacagtggg tggctgtcgg
    12181 tgcctctgat aagccgtatc cttccacgag agacgccccg gttagttttt caaaattttc
    12241 gcgaaccgcc tccatcagtg gcgcagaacc agagatacag gctttgatgc ggtttaattg
    12301 ataatctccc ctttttacgc cgggatggtt attgatcagg ttaaaaatgg acggcaccgc
    12361 agggaaataa ctgactggat acttcgtaat tgcgtgtaac atttcatcca catcgcccgg
    12421 tttttgcaag agcaccagcg ttgctccagt ggcgattccc agtaataaac ccaataccag
    12481 tccatacacg tgatacaaag gaatggcgac caggtaggtt tccttgccgt catcgagatt
    12541 caccaaccag ttcttgaatt gggtaacatt ggcaacaagg tttcgatgtg tcccaatcgc
    12601 cgccttggga a
//
LOCUS       DAOSPM010000051        12472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_5058_length_12472_cov_15.220665, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000051 DAOSPM010000000
VERSION     DAOSPM010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12472
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5058_length_12472_cov_15.220665"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLW25_05840"
     CDS             complement(<1..278)
                     /locus_tag="PLW25_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54044.1"
                     /translation="MTEQMFMSPEWMAETEKILRAMITPQTTHNASLSLTMTVENLPS
                     GETKTLVFATEKGEMKTFRLADPGSVNTEFSISGDYRVYEKLFKGQLEP"
     gene            complement(311..1642)
                     /locus_tag="PLW25_05845"
     CDS             complement(311..1642)
                     /locus_tag="PLW25_05845"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HOS54045.1"
                     /translation="MPEAIFQFPRGFLWGCATAAHQVEGGNSNNNWSKWEETPGKIVN
                     GHRSGLACDWWGGRWLEDLERARDSGQNAHRLSIEWSRIQPEPDRWDDEALLKYTEML
                     RWMTRNGITPLVTLHHFTDPLWLSERGGWESEDTPALFARFCRRVVKTLKDYVNLWVT
                     INEPNVYILSGWVEGVFPPGKQDLKLAARVFTNLVQGHSAAYRIIHELQPEARVGVAT
                     NYRSFAPAHPGSPLDKLPLGFVNQVFNRAFNNCLVDGKVDLVFKKYKLPQAAGTQDFI
                     GLNYYTRDVVSFNLLKPGAFFHQRAFPAGAALSENHFIANVPAGMWDGLKWAHAYGIP
                     IIVTENGIEDSHDTLRPRYLVEHLHQVWRAANYNYQLKGYFHWSLVDNFEWERGWTQR
                     FGLWGLDVDTQQRNRRLSVDVYERVCRENGLSSHMVAELVPDVLDALFPDH"
     gene            complement(1735..>1916)
                     /locus_tag="PLW25_05850"
     CDS             complement(1735..>1916)
                     /locus_tag="PLW25_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOS54046.1"
                     /translation="RSPVRISVFHALAEAKAQALLESAAKRLKADEAILSYVSPVIGC
                     HVGPGTLSIAYMAGM"
     assembly_gap    1917..2016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2355..2651
                     /locus_tag="PLW25_05855"
     CDS             2355..2651
                     /locus_tag="PLW25_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54047.1"
                     /translation="MIILISMLLTACGGDSLRGSWREPNSGIDMEIRNDGKLVMSLNG
                     QSITMNYTLEDPDVIILVGSLDGTIPDQKMTYRVEENKLTFTLDGADTVFYRVK"
     gene            2787..3998
                     /locus_tag="PLW25_05860"
     CDS             2787..3998
                     /locus_tag="PLW25_05860"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOS54048.1"
                     /translation="MMQKKILFKILVVVAVFTLGLTSCNLMGYIQKNDADDFTQIETM
                     AAATISARLTQAAFDELAMQLTQQAPTLSLPTSQLPLSSSTPIPTSTPIASFTPVATS
                     IPPTATPIPIPCNAAKYLADVTIPDWSTVYAGEAFVKTWQVKNVGTCSWTKDYHIYFA
                     DGKKLDAPTSVAFPKVVNPGETVELSVPMVAPSDKGSFTGSWMLEAANGVRFGVGYTY
                     NVPLTVNIKVENMPAPKDPHTRYDLVKEMCSASWRTNAGDIGCPSAKVDTDKGSITRS
                     YAPVIAGGYKDDEGALYTVPAKGGDGMILGRFPKFKLLSGDRFRALLICADKAQKCSV
                     TYELLYKVSGSDESGSLGTWDVNYSNSTQVDVNLDSLADKEVHLMLKVYSKGDSTDDV
                     ALWMAARVTNP"
     gene            complement(4075..5745)
                     /locus_tag="PLW25_05865"
     CDS             complement(4075..5745)
                     /locus_tag="PLW25_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HOS54049.1"
                     /translation="MKRIERVEHWRKARQYTSEQLEQAKIVLEEVRAGVRLDRALRAN
                     PMPDGALIAKHILVAAYRELVAEGEWPEDPSFLSRIRMKPVRTLSGVTTVTVLTKASP
                     CPGECIFCPSEQQMPKSYLSDEPGAARAYQNRFDPFDQVSSRIHEYEAVGHPNDKIEL
                     LVLGGSWSCYPRAYQEWFIRRCFHAMNADSTLPGDEPVDTSEPPAGARPQDDLVELRK
                     AHTRNENALHRNVGLVIETRPDDIDRDELVWLRRLGVTKVQMGAQSLDDRILQLNRRG
                     HSSADTLRAVTLLRAAGFKIVLHWMPNLLGATPASDREDFARLWKDGYAPDELKIYPT
                     QLLESAELYHYWQRGEYTPYTTEQLIDLLADLKPLIPPYCRVNRVIRDIPSFHVVEGN
                     RRTSLRQDVQRELQRRGTACRCIRCREVKGESVSSAELAFSDYVYQPLTSEEHFLSYT
                     TPEDRLAGYLRLSFALPGAPDLAIPELDSAAIIREVHVYGQSLPVGTERDGAAQHSGL
                     GTSLLDHASRITRARGLSRLAVIAAVGTRAYYEKRGFHQQELYMVKEL"
     gene            5765..6526
                     /locus_tag="PLW25_05870"
     CDS             5765..6526
                     /locus_tag="PLW25_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOS54050.1"
                     /translation="MRQDIFQRLLNINREFYQSFGQAFAQTRRRVQPGVRRALAQWIT
                     NGDWLDIGCGSGTLSREWRSAGLKGSYTGVDFSQPLLKEAAALQQNPLESDELTVSFL
                     QADLTTPDWTSVLPRRMYDGVVAFASLHHIPSAAYRKQLLDTIARMIKPGGLLVLSVW
                     QFQHSPRLMARVLPWRLADMAEEDLEEGDTLLDWRYHPGESGAAPGLRYVHLFTPEEL
                     QALARKTGFEVVTAYESDGSGGRLGLYQVWQRAAL"
     gene            complement(6521..6934)
                     /locus_tag="PLW25_05875"
     CDS             complement(6521..6934)
                     /locus_tag="PLW25_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOS54051.1"
                     /translation="MTRLLYLPDDATVIQLEVDLSPAELVAAVNAGLRPLPILRTAPA
                     GKLSASQVNATVVIAPLRSPGGPRRNSVNANLTRRQRQVLELQSRGFNSAEIAAMLNL
                     SRRAVNYHLNQVRQRIRGELPELLKSDFDELESLL"
     gene            complement(7174..8622)
                     /locus_tag="PLW25_05880"
     CDS             complement(7174..8622)
                     /locus_tag="PLW25_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOS54052.1"
                     /translation="MESKAGAQISQKAFIQSAVILLVLMIAAGILTRVIPAGSYQRYQ
                     VEGRSLIDPASYQVIERPDYPIWRWFTAPVEVLWGEDSLIIITIIVFIVLVSGAFAVL
                     EKSGILTALLGKIVKRFRSRRYVLLLVIALFFMLLGGFFGLLEEIIPLVPLLIALSYS
                     LGWDALVGLGMSILATNIGFSAAITNPFTIGVAQRLAGLPLFSGALLRLPVFLVFYAA
                     LAWFLLSYARRIDRDPASSLLYKEDQEKGTRKPLFDLADTDSRSGIGKAALVFGFFVL
                     LILAVLLTGPFVPTIADYTLPLVGVLFFAGGLVSGFVSGCPGRLIGRALKEGILGLAP
                     GIPLILMAASTKHIVSNGKVIDTLLYQGSHLLEGASPLSAVLIIFLLALVVEFFVASA
                     GAKAVLMMPILLPLADLVGVTRQLTVSAYCFGDGFTNLAYPTNAILLICIGLASISYG
                     KWIRFTAKLWLMVFPLVVFFLWIGSLIHFGPF"
     assembly_gap    8859..8958
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8959..9219
                     /locus_tag="PLW25_05885"
     CDS             <8959..9219
                     /locus_tag="PLW25_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOS54053.1"
                     /translation="KSISIRDQRTRWGSCSSQGALSFNWRLVMVPPAVLEYVVTHELA
                     HRKQQNHSRAFWQVVALYFPHFKAARTWLRANATLLRPEDTI"
     gene            9246..10265
                     /locus_tag="PLW25_05890"
     CDS             9246..10265
                     /locus_tag="PLW25_05890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS54054.1"
                     /translation="MEFTHHTEFPYEMATEWNALLAESATHVPFLRCEYLADWWATRG
                     GGEWPDDSLLAIITAREDGRLIGIAPLFIASHEDKKKLLLLGSIEISDYLDVICRAEE
                     INRFLHGLLRFIREQFVATGQITGLDLYNIIEDSPTLKALEESAKTLDLEMRVRKLQH
                     SPFIPLPGNWETYLERLEKKQRHEIRRKMRRALEGDARVDLYFTQDEGNLEDDMEDFL
                     ELMAQDEAKEDFLSPMMREQMKSVMRCAFDQNCLQLAFLEIGGQKAAAYLSFDFLNRI
                     WVYNSGIDRRYMEYSPGWVLLGYLLKWANENGRETFDFMRGNEDYKYKFGALDRFVMR
                     ASLEF"
     gene            complement(10330..11808)
                     /locus_tag="PLW25_05895"
     CDS             complement(10330..11808)
                     /locus_tag="PLW25_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HOS54055.1"
                     /translation="MQFFLPLVFLILVLAAVIAARTFLLIRSAGHKPLPFQSKLPDPP
                     LDRQKIAEHLSAAIQIETVSHEDPAKNRTENFDRLHDLLQRTYPRLHGSLSREIVQGF
                     SLLYTWQGSDPALEPVVFMAHQDVVPIDEHTLEQWTHPPFSGRIADGFIWGRGSLDIK
                     NQLIAVMEAVELLLEQGYQPVRTVLLSFSHDEEVLGHGAPAIVRHLKEKGISVHAVLD
                     EGNAVLDGVLPGFKGHTAPIGVAEKGYLSLKLTVEASGGHSSTPAAETAIGILARAID
                     RLQSHPFPYRLNTVYPMFKGLSPAAPRLLQVAFANLWLFGGLVRRQLAAQAETAAAIH
                     TTTAPTLFHGGVKDNVLPTHAEAVVNFRLLPGDSVAEVTEQVQSVINDSRVRIEPLPE
                     YAWEPSPVSPTDTPAYQHIVSVLNELFTDIACAPSNMLGATDARNYHAISSRVYRFSP
                     LLLHKKDLERIHGINERISLENMHKMVSFFHRLIQRWSSGEM"
     gene            complement(11956..12396)
                     /locus_tag="PLW25_05900"
     CDS             complement(11956..12396)
                     /locus_tag="PLW25_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOS54056.1"
                     /translation="MATVASILKKKGSQVYSVSPETSIIDCLKLMSEKKIGSVLVMQD
                     EKVVGIFSERDYAWRGILKGNHEHTPVQRVMTEKVYYVSPEQSTDTCMAQMSDKHIRH
                     LPVLDKGKVVGVISIGDVVTSMLQDKETLIKGLENFILSNEIKL"
BASE COUNT         2831 a         3257 c         3524 g         2660 t
ORIGIN      
        1 ggctccagtt gacctttgaa caacttttca tacacgcggt aatctccgct gatggagaac
       61 tcggtattca cgctccccgg gtcagccagc cggaaggttt tcatttcccc tttttcggtc
      121 gcgaagacca gggtctttgt ctcgccgcta ggaaggtttt ccactgtcat cgtgagagac
      181 aggctggcgt tatgggtggt ttgcggggtg atcatcgcgc gcagtatctt ctctgtttct
      241 gccatccatt cagggctcat gaacatctgt tcagtcaagg tcaacctcct cttctattta
      301 ccagcagtgt ctaatggtcg gggaacagcg catccaaaac atcgggcacc agttctgcga
      361 ccatgtggct ggaaagcccg ttttcgcggc aaacacgttc atacacatcc acgctgagcc
      421 ggcggttgcg ctgctgggtg tccacatcca acccccacaa gccaaagcgc tgcgtccaac
      481 ctcgttccca ttcaaagttg tcaactaagg accaatggaa atagcccttt agttgatagt
      541 tataattggc cgcccgccac acctggtgca ggtgttccac cagatagcga gggcgcagcg
      601 tgtcgtgcga atcttcaatt ccgttctcgg tgacgatgat cggaatcccg tacgcgtgcg
      661 cccatttgag cccatcccac atgcccgcgg gcacgttggc gataaaatgg ttctcgctca
      721 gcgcggcgcc tgctggaaac gcccgctggt ggaagaaagc gcctggtttc aacaggttga
      781 aagacaccac atcgcgggtg taataattta gcccgataaa atcctgtgtg ccggcagcct
      841 gaggtaattt atactttttg aaaaccaggt ctacttttcc atccaccagg cagttattga
      901 aagcccggtt aaagacctgg ttgacgaaac ccagcgggag cttatccaat ggagagccgg
      961 ggtgggcagg ggcgaagctg cggtaattgg tggcaacccc cacgcgcgct tcgggctgaa
     1021 gctcatggat gatgcggtaa gcggcgctgt gcccctggac caggttggtg aataccctgg
     1081 cggcgagttt caggtcttgc tttcccgggg gaaacacgcc ctccacccaa ccgcttaaaa
     1141 tgtagacgtt tggctcgttg attgtgaccc acaggttgac atagtctttg agcgttttga
     1201 ccacgcgacg gcaaaagcgg gcgaaaagag cgggggtgtc ttcgctttcc caaccccctc
     1261 tttcactcaa ccataatggg tcagtaaaat gatgcagcgt gaccagcggc gttataccgt
     1321 tgcgcgtcat ccagcgcagc atttccgtgt acttcagcag ggcttcatcg tcccagcggt
     1381 cgggctctgg ctgaatgcgg ctccactcga ttgaaagacg gtgggcgttc tgtccgctat
     1441 cgcgcgcccg ttccaggtct tccagccagc gaccgcccca ccaatcacag gccagccctg
     1501 aacgatgacc attgacgatt tttccggggg tctcttccca ttttgaccag ttgttgttgc
     1561 tgtttccacc ctccacctgg tgggcggcgg tggcgcaacc ccagagaaag ccgcggggga
     1621 attggaagat cgcttcgggc atgcgctgct cctgtattct ttgtgattgc agctaattat
     1681 agccgaaaca aaaaccccgg ctcaaccggg gttaaaatgc gttggctgaa aggatcacat
     1741 ccctgccata taggcgatgc tcagcgtgcc tgggccgacg tgacagccga tcacagggct
     1801 gacgtaactg aggatggctt catcagcctt cagtcgttta gcggctgatt caagcagcgc
     1861 ctgggctttt gcttctgcca gcgcgtggaa cacagagatg cgcaccggtg agcggcnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntccg tctcgataac ttttaccatc
     2041 ccattgcagg gtgagcggca acacgtgaat gccgaactca tctacatact gctgtggcag
     2101 ataggcggtg gaatcggtca cgatagctac ttttttcatg agtcactcct gggaaagtat
     2161 ctatttgcaa agatgaaaca taggttaagt atacgtaaga tctcaaatcg cgaaaatggg
     2221 tcgaaggtca tattttcacc tctgagagca gcggcgctac ccttgccgga tcacgactcc
     2281 atgagtaaac taagcttgaa aaaagcgagg taagccaaaa tatgggaagc agcgctcgaa
     2341 caaaactctt tataatgatt attttgattt ccatgctgtt gacggcatgc ggcggcgatt
     2401 cattgcgcgg ctcatggcgc gaacctaact cggggatcga tatggagatt cgcaatgacg
     2461 gcaaactggt gatgagcctg aacggtcaga gcatcaccat gaactatacc ctcgaagacc
     2521 ccgatgtcat catcctggtg ggcagcctgg atggcaccat ccccgaccag aaaatgacct
     2581 atcgcgtaga agagaacaaa ctcaccttca cactggacgg cgctgacaca gtcttctacc
     2641 gtgtgaaata aaaaacaccg ctccggcctg tcgcgccggg cgttccgatt ctcactttaa
     2701 gtagtaatat gaaggagaaa ctcttgatca aaggcgggaa catttcgctc gcggttgcgt
     2761 cttaagcaag atataaacgg aggtttatga tgcaaaagaa aattctcttt aaaatattgg
     2821 tcgttgtggc ggttttcacg cttggattga catcctgtaa cctgatgggg tacattcaga
     2881 aaaatgacgc ggatgatttt acccaaatcg agacaatggc ggcggcgacc atctcggcgc
     2941 gtttgacaca ggcggctttt gacgagctgg ccatgcaatt gacgcaacag gcgcctacac
     3001 tgtctttgcc cacatcacag ctgccgctgt cctcaagcac gccgatcccc accagcacgc
     3061 ctattgccag tttcactcct gtcgccacga gcatcccacc cacagcgacg ccgattccca
     3121 tcccctgcaa tgctgccaag tacctcgctg atgtcaccat tccagattgg agcacggtct
     3181 acgcgggcga agcgttcgtg aagacctggc aggtaaaaaa tgtgggtacc tgttcctgga
     3241 caaaagatta ccatatctac tttgctgacg gcaagaagct ggacgcgcct acttcagttg
     3301 ctttccccaa ggtggtaaac ccgggtgaaa ccgttgagct ttccgtgccg atggtggctc
     3361 cttctgataa aggcagcttc accggcagct ggatgcttga agcagctaat ggcgtgcgtt
     3421 ttggcgttgg ttacacctac aacgttccgc tcacggtgaa catcaaggtg gagaatatgc
     3481 ccgcgcccaa ggacccccac acccgctatg acctggtcaa ggagatgtgc tccgcctcgt
     3541 ggcgcacgaa cgctggcgat attggctgcc cgagcgccaa ggttgataca gacaaaggct
     3601 caattactcg ctcttacgcg ccggtgatcg ctggaggtta caaggatgac gaaggcgcat
     3661 tgtacaccgt gcccgcaaaa ggcggcgatg ggatgatcct gggcaggttc cccaagttta
     3721 aattactgtc aggagaccgt ttcagggcgc tgctgatatg cgctgacaag gcgcagaagt
     3781 gttccgtcac gtatgaattg ctttataagg tgtcggggag cgatgagagc ggatcgctgg
     3841 gtacgtggga tgttaactac tcaaactcca cccaggtgga cgtgaacctg gattcgctgg
     3901 ccgataaaga agttcactta atgttgaaag tgtacagtaa aggagactcg acggatgacg
     3961 tcgcgttgtg gatggccgcc agggtgacca atccataaga tgtaaaagac ccaacacgag
     4021 gctgcctcac ctattgaagc agcctcgtgt tttttgaaat aagtcgttca gacgctacaa
     4081 ctctttcacc atatagagct cttgctgatg aaaaccacgt ttttcataat aggcgcgcgt
     4141 acccaccgcg gcgatcaccg ccaggcgcga aagaccccgc gcgcgtgtga tacggctcgc
     4201 gtgatcgagc aggctggtac ccagcccgct gtgctgcgcc gccccgtcgc gctccgtgcc
     4261 caccggtaac gattgaccgt agacgtgcac ctcgcgtatg atggcggcgg aatcgagttc
     4321 gggaatggcg agatcaggcg cacccggcag cgcgaatgac agccgcaggt agcctgccag
     4381 ccgatcttca ggggtggtgt aagaaagaaa atgttcctcg gaagtcaaag gctggtaaac
     4441 ataatcagaa aaggcgagtt cagcggaaga cacagactct cccttcactt cgcggcagcg
     4501 aatgcagcgg caggcagtgc cgcggcgctg cagttcacgc tgcacatcct gccgcagact
     4561 ggtgcgccgg ttgccttcta ccacgtgaaa agatgggatg tcccggatga cgcggttgac
     4621 gcggcaatag ggagggatga gaggtttgag gtccgccagc aaatcaatca gctgttcggt
     4681 ggtgtaaggc gtgtattctc cacgctgcca gtagtggtaa agctcagcgg attcaagtaa
     4741 ttgggtgggg taaatcttaa gctcatcggg ggcgtacccg tctttccaaa gacgggcaaa
     4801 atcctcgcgg tcagatgcgg gtgtggcgcc cagcaggttg ggcatccagt gcagcacaat
     4861 cttgaatccg gcggcgcgca gcagcgtgac ggcgcgcagc gtatctgcgc tgctatgacc
     4921 gcgccggttg agttgcagga tgcggtcatc caaactttga gcgcccatct gcactttggt
     4981 cacacccaga cgacgcagcc agacgagctc atcccggtcg atatcatccg gccgggtttc
     5041 aatgaccagc cccacgttgc ggtggagcgc gttttcattg cgcgtatggg cttttcttaa
     5101 ttcaaccagg tcatcctggg ggcgggcgcc ggcaggtggc tcggaagtgt cgacaggctc
     5161 atcaccaggc agggtgctgt ccgcgttcat cgcgtgaaag cagcggcgaa tgaaccactc
     5221 ctggtaggcg cgcggatagc agctccagct gccgcccagc accagtaatt cgatcttatc
     5281 attggggtgc ccgacggctt cgtactcgtg gatacgcgag cttacctggt caaaggggtc
     5341 aaaccggttt tgataggcgc gggcggcgcc gggttcgtcc gacagatagc tcttgggcat
     5401 ttgctgctcg ctggggcaga agatgcactc tccagggcag ggagacgcct tggtaaggac
     5461 ggtgaccgtg gtgacgcctg aaagcgtccg tacaggtttc atgcggatgc gggataaaaa
     5521 cgaggggtct tcaggccatt caccttccgc caccagctcg cggtaagccg ccaccaggat
     5581 gtgtttggcg ataagggctc catcgggcat ggggttggcg cgcagcgcgc gatccaggcg
     5641 gacgccagcg cgcacttctt cgaggacgat tttcgcctgt tccagctgtt cggaggtata
     5701 ttggcgcgcc ttgcgccagt gctctacgcg ttcaattcgt ttcatcggaa agtataatgg
     5761 ggctatgcgg caagatatct ttcagcgctt actgaacatc aatagagaat tctaccagag
     5821 tttcgggcag gctttcgccc agacacgacg ccgcgtacaa ccgggtgtgc gccgcgcgct
     5881 ggcgcaatgg atcacaaatg gcgactggct ggatattggc tgcggcagtg gtacgctttc
     5941 acgggagtgg cgcagcgcgg gcttgaaagg cagctacacg ggcgtggatt tcagccagcc
     6001 gctgctaaaa gaagccgctg cgctgcaaca aaacccgctg gagagcgatg aactcaccgt
     6061 ctcctttctg caggctgacc tgaccacgcc agattggaca tccgttttac cgcgccggat
     6121 gtatgatggc gtggttgcct ttgcctcgct gcaccatatc ccatccgctg cttatcgaaa
     6181 acagctgctg gatacaattg cgcgcatgat taaacccggc gggctgctgg tcctttcagt
     6241 ttggcagttt cagcacagcc ccaggttgat ggcgcgtgtg ctgccctggc gcctggcaga
     6301 catggctgaa gaggacctgg aagaagggga caccttgctg gactggcgtt atcacccggg
     6361 ggagtcaggc gcggcgccgg ggctgcgcta tgtgcacctg ttcacgcctg aggagcttca
     6421 agcgctcgca cgcaaaacgg ggtttgaggt cgtcacggcg tatgagtcag atggcagcgg
     6481 gggcaggctg ggattatacc aggtgtggca aagggcggct ttataaaagg ctttcaagtt
     6541 catcaaagtc ggatttaagc agctcgggga gttcgccgcg gatgcgctga cgcacctggt
     6601 tgaggtgata gttgaccgcg cgcctggaga ggttgagcat ggcggcaatt tcagcgctgt
     6661 tgaaaccacg cgattgcagc tccagcacct ggcgctggcg gcgggtgagg ttggcgttta
     6721 cactgttgcg gcgcggaccc ccgggtgagc gcagcggagc aatcaccacc gtggcgttca
     6781 cctggctggc gctcaatttg ccagccgggg cggtgcgcaa aatgggcagc ggtcttaacc
     6841 cggcgtttac cgcggccacc agttccgccg ggctgagatc aacttcaagt tggatgacgg
     6901 tcgcgtcatc cggcaggtat agcaggcggg tcaatgtggt ttccggcagg agtgaggacg
     6961 tgtaacgtgc atgatgaatc acttagatag aattagaact aacattctat atattaacct
     7021 gtaagaagca ctttgtcaag acaggcgtgg ataaactgaa aactcatccg gctggatggt
     7081 tgaagacccc tggaggcagt gaatttaccc ggcagcgggt gcgcgttcac accatctaaa
     7141 aaaaaagcaa gtggagatgg taaaaacagc cgcctaaaag ggtccaaagt gaatcaggct
     7201 gccaatccac aagaaaaaca cgaccagggg gaagaccatc agccagagtt tcgcggtgaa
     7261 gcgtatccac ttaccatagc tgatggaagc caacccgatg cagatgagca ggatggcatt
     7321 ggtgggatac gccaggttgg tgaaaccgtc gccaaaacag taggcgctga ccgtgagctg
     7381 acgcgtcacg cccaccaggt cagccagtgg taagaggatc ggcatcatca aaacggcttt
     7441 cgcgccggcg gatgccacaa agaactccac caccagcgcc agcagaaaga taatgagcac
     7501 cgcggacagc ggcgaagcgc cttccagcag gtgcgacccc tggtacagca gggtgtcgat
     7561 gacttttccg tttgaaacga tgtgtttggt gctggctgcc atcaggatga gcgggatgcc
     7621 gggagccaga cccaggatcc cttctttaag cgcgcgaccg atgagcctgc ctgggcagcc
     7681 ggagacaaat ccgctcacca gcccgccggc gaagaacaac acgcccacca gcggcagggt
     7741 gtaatcagcg atggtgggga caaacggacc tgtcagcagc actgccagga tgagcaatac
     7801 gaaaaatccg aataccagcg ccgctttgcc aataccagat cggctatccg tatctgccag
     7861 gtcaaaaagt ggcttgcgcg tgcccttctc ctggtcttct ttatagagca gtgaagaggc
     7921 cgggtcgcgg tcaatacgcc gcgcgtatga aagcaaaaac caggccagcg cggcgtagaa
     7981 aacgaggaac acgggaaggc gcagcagcgc gcctgagaag agcggcagcc ccgccaacct
     8041 ttgcgccacg ccgatagtga agggattggt gatggcggca gaaaacccga tattggtggc
     8101 cagtatgctc atgcccagac ctaccagcgc gtcccaacct aatgagtaag aaagcgcgat
     8161 caataaaggc accagtggaa tgatctcttc cagcaggccg aaaaagccgc ccagcagcat
     8221 gaaaaacaag gcgatcacta aaagcagcac gtagcgccgc gaacggaagc gtttaacgat
     8281 cttccccagc aaggcggtga gaatgccgct tttttccagc accgcgaatg ccccgctgac
     8341 cagaacaatg aaaacaatga tggtgatgat gatcaggctg tcttcgcccc atagcacttc
     8401 cacgggcgcc gtgaaccagc gccagatcgg ataatccggg cgttcgatga cctggtaaga
     8461 tgcaggatcg atcaggctgc gtccctccac ctggtaacgc tggtaactgc cggcggggat
     8521 cacccgggtg agaatgcccg cagcaatcat caataccagt aaaatcacag cggattggat
     8581 aaaggctttt tggctgatct gcgctccggc tttggattcc ataggtgacc atccttatga
     8641 ggtgaagtct ttcataagtg tagacctgga gatgacctgt gtcaaattgg cggctaaaaa
     8701 agtggatgcc ggggaaaagc gctggtatga ttatgtatga aaatgaccga acccatagaa
     8761 tctgtcacca ccatagcggg gcgccgaata ccctaccgcc tggttcgctc actgcgcgcc
     8821 cgccggatgc ggatgaccat taaagaaagc caggtgacnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnaa gagcatttcc atccgcgacc aacgcactcg ttggggcagc
     9001 tgttcgagcc agggcgcgct ttcatttaat tggcgattgg tgatggtgcc tcccgcggtg
     9061 ctggaatacg tggtaacgca cgagctcgcg caccgcaaac agcagaatca ttctcgggcg
     9121 ttttggcagg tggtggcgct ttactttccg cactttaagg cagcccggac gtggctgcgc
     9181 gcgaatgcca ccctgctgcg acccgaagac acgatataga ccctgaaaca ggtataataa
     9241 gttcaatgga gttcacccat cacactgagt ttccctatga aatggcaacc gaatggaacg
     9301 cgttgctggc tgaaagcgcg actcatgtgc cttttttacg ctgcgagtac ctggcggatt
     9361 ggtgggcaac acgaggtgga ggtgaatggc cggatgattc tctgctggca atcatcaccg
     9421 cccgcgaaga cggacgactg ataggtatcg ctccgttgtt catcgcctcg cacgaggata
     9481 aaaaaaagct gctgctgttg ggcagtattg aaatcagcga ttatctcgat gtcatttgcc
     9541 gcgccgagga aattaaccgg tttttgcacg gactcttgcg ctttattcgg gagcaatttg
     9601 tcgccacagg gcaaatcacc gggctggacc tctacaacat catcgaggat tctccaaccc
     9661 ttaaggcgct cgaggaatca gcgaagaccc tggacctgga gatgcgcgtg cgcaaacttc
     9721 aacactcgcc gttcatccca ctgccgggta actgggaaac gtacctggaa aggctcgaaa
     9781 aaaagcaacg gcatgagatc cgccggaaaa tgcgccgcgc cctcgaaggg gatgcgcggg
     9841 tagaccttta ctttactcaa gacgaaggaa acctcgaaga tgatatggag gactttctgg
     9901 agttgatggc gcaggacgaa gccaaggaag atttcctctc gccgatgatg cgcgagcaga
     9961 tgaaatccgt catgcgctgt gcctttgacc agaactgcct gcagctggct tttctcgaaa
    10021 tcggcggaca gaaagcggct gcttacctga gcttcgattt tctcaaccgc atctgggtgt
    10081 ataactccgg tattgaccgg cgctatatgg aatattcacc gggttgggtg ctgttgggtt
    10141 acttgctgaa atgggctaat gaaaacgggc gtgaaacctt tgattttatg cgcgggaatg
    10201 aagattacaa atacaagttt ggcgcgttag accgttttgt gatgcgcgcc agccttgaat
    10261 tctaagcagg atgtataaaa aaaagcgggt gcttgacagc cacccgcttt taatgcccct
    10321 ggtttttact cacatttctc cgcttgacca acgctggata aggcggtgga agaaagacac
    10381 catcttgtgc atgttttcta atgagatgcg ttcgttaatt ccgtgaatac gttcaaggtc
    10441 ttttttgtgt aacaagaggg gcgaaaaccg atacacacgg gaagaaatgg cgtggtaatt
    10501 acgcgcgtca gtggcgccaa gcatgttgct tggcgcgcag gcgatatctg tgaagagttc
    10561 attgagcacg ctgacgatat gctggtacgc cggggtatcg gtaggggaaa cgggagaggg
    10621 ctcccaggcg tattcgggga gcggttcgat gcgcacgcgg ctgtcgttga tcactgattg
    10681 aacctgctcg gttacctcgg cgactgaatc acctggcagc agcctgaagt tcaccactgc
    10741 ctcggcgtgc gttggcagca cgttatcttt tacgccaccg tgaaagagcg tgggcgcggt
    10801 ggtggtatgg atggcggcgg cggtttcggc ttgcgctgct agttgacggc gcaccagacc
    10861 gccaaataac cacaggttcg caaaagccac ctgcagcaac ctgggggcgg ccgggctgag
    10921 acccttgaac attgggtata cggtattcaa ccggtaagga aaggggtgag attgcaggcg
    10981 gtcaatggcg cgcgccagaa taccgatcgc agtttcggcc gcaggggtag aagaatgacc
    11041 gccgctggct tctacggtca gtttaagtga gaggtagcct ttttcagcca cgccgattgg
    11101 cgcggtatga cctttaaagc caggcagtac accatccagc accgcattgc cttcatcaag
    11161 aacagcgtgc acgcttatcc ccttttcttt gagatgcctg acgattgctg gcgcgccgtg
    11221 ccccaacacc tcttcatcgt gtgaaaaaga aagcaagacc gtccttacgg gttgatagcc
    11281 ctgctccagc aggagttcga ccgcttccat tacagcgatc aactggttct taatatccaa
    11341 acttcccctg ccccaaatga aaccgtcggc aattcgcccg gagaaaggcg gatgagtcca
    11401 ttgctccagc gtatgttcat cgataggcac cacgtcctgg tgggccataa agaccacggg
    11461 ttcaagagcg gggtcgctgc cctgccaggt gtacagcaag ctgaagccct gcacgatttc
    11521 gcggctgaga gacccgtgaa ggcgggggta ggtgcgttgc agcaggtcat gcagccggtc
    11581 aaagttttcg gtgcgatttt tcgctgggtc ttcatgcgat acggtttcta tttgaatcgc
    11641 agcagagagg tgctccgcga ttttttgcct gtccagtggt ggatcgggca gcttggattg
    11701 aaaaggtaat ggcttgtgac ccgcggagcg aatcagcagg aacgtacgag cggcgatgac
    11761 agccgcgagg acgagaatca aaaagaccag tggaaggaag aattgcatac gcacctcatg
    11821 tccagcggca aaggttattc ctgcacgggt cggcagtgat gaccagagca gttatcaggg
    11881 ggttgaagat ggctttggcg cccagaaccc cggcaatctg aaccgccggg tgctacgcgt
    11941 gttccatcca attttttata gtttaatctc gttgctcagg atgaagtttt ccagaccttt
    12001 gataagcgtt tctttgtcct gcagcatcga ggtgaccaca tcccctatcg agataacgcc
    12061 aacgactttc cctttgtcta acaccggcag gtggcggatg tgcttatcag acatttgcgc
    12121 catgcaagta tcggtcgatt gctcggggct tacgtaataa actttttcgg tcatcacccg
    12181 ttgaaccggt gtgtgttcat gattcccttt cagaattccc cgccaggcgt aatcccgctc
    12241 tgagaaaatg cccaccactt tttcatcctg cattaccagc acggatccga ttttcttctc
    12301 gctcatcagt ttcaaacaat caatgatgga agtctcgggt gagacagagt aaacctggga
    12361 gcctttcttt ttgagaatgg atgcaactgt agccataatc acctccgtaa cgtgttgtct
    12421 tgttgagcag tgcgttaata atagcctatc cttgaatgac ttgtcaatga cc
//
LOCUS       DAOSPM010000052        12416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_5083_length_12416_cov_14.628590, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000052 DAOSPM010000000
VERSION     DAOSPM010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12416
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5083_length_12416_cov_14.628590"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PLW25_05905"
     CDS             complement(<1..1064)
                     /locus_tag="PLW25_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOS54057.1"
                     /translation="MERTLIKDLRGKIDQTVKLQGWLQTLRDQKKMQFLILRDPTGLV
                     QVAHWKPNDETLASNIAGLGAETALTITGKVIDNPVVKLNQLEVQLESLVVENLAESP
                     LPFDPFAETLPALDYRLDWRYMDLRRAYNNLLFQVQTTMEMAMREYWLKHDFIEIHSP
                     KLMGTPSESGAELFSLDYFERKAYLAQSPQFYKQMAMAAGFERIFEIGPVFRADPSFT
                     ARHMTEFTGVDLEISWIDSHEDVMAFEENWLQYVYARVKELHGEAIKETFGVELVVPQ
                     VPFPRIPMRDAVEILKSRGYVLPADKKGDIDPGGEREIASYVKEKYDHDFVFLTDWPI
                     GVRPFYHMRLAGDETLTRSF"
     gene            complement(1096..2094)
                     /gene="meaB"
                     /locus_tag="PLW25_05910"
     CDS             complement(1096..2094)
                     /gene="meaB"
                     /locus_tag="PLW25_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOS54058.1"
                     /translation="MVDLDAVTRGNRLELARLLTEVENQTPAGEQALDRLFARAGKAH
                     LVGITGAPGTGKSSLVSCLAKNIRVAQKQTVAIVAVDPSSPFTGGAILGDRVRMRELA
                     GDPGVFIRSMASRGELGGLASATAGVVQVLDAAGFDFILIETVGAGQSEVDIACLAYT
                     TLVVESPGLGDDIQAIKAGILEIADILVVNKSDLPGAENTARALRANLDLGYHLHMNH
                     DGAHRMTIKENEVRNTTAWTPPIIQTIATQHNGIDEVIAEILRHREYLIDTGLREKRE
                     QDRLRSEVYHLLQTGLVKRWQQLTPPQVFDRLLQQVFARELSPRAAIDSLLNGSYN"
     gene            complement(2110..2511)
                     /locus_tag="PLW25_05915"
     CDS             complement(2110..2511)
                     /locus_tag="PLW25_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOS54059.1"
                     /translation="MTEKKIRVLIAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQS
                     PEMIAEAALQEDVNVIGLSILSGAHLVLVPRVIDLIRANGQEEVKVFVGGIIPEEDIP
                     VLTQAGVSAIYGPGTPTTKVVQDIRDAFSAV"
     gene            3454..4083
                     /locus_tag="PLW25_05920"
     CDS             3454..4083
                     /locus_tag="PLW25_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54060.1"
                     /translation="MTYERKGYVHGAWVIKIGQNDLVIKASENRSFPELDQLYTPRVK
                     NPKHWDDYAEKLIPDAEKQLYSLLDLPYYPVEKRVKLSQLIERTKWKFAWTYARTYPH
                     EYTTKALCEAADHAKLIDCIEKYGIIQRFGESYRKYFYFEDRKYWHMGEPYSDDPELR
                     PNVINRTWVDVRRHAENVKHRWTVEEVELQQRLWEIQLEKSTDRNKPKK"
     gene            complement(4143..5219)
                     /locus_tag="PLW25_05925"
     CDS             complement(4143..5219)
                     /locus_tag="PLW25_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54061.1"
                     /translation="MGGEFYYDSSWQVNTSVVDTSHLTFLNGGKACLIVIGDYLRAHG
                     INEILLPSYLCPSILNTLEKHGLGCDFYPVQPDLTIDLEEMARKAEGHRAVYFIHYFG
                     CPREPAELDLLRSLQAGGTLLIEDNAQCGFSPTVCGDFAFNSMRKLCAYDGGYLDSRF
                     DLLPYIEKRAGLKNRRLPLIRDYRSRLADYLFNSKGDRNELQQLYELAENHYESDEVV
                     EGDIVERHHIEHLDWDGIRKKRRDNYRYLVSLVEGLPGITPIFPILPQGVTPLSLPVY
                     VNIPSRDQLGDALGDAGIGLTIHWEGLVRDPRVSGNTAVVDMASRMLSLVIDQRFSHK
                     HMDYQAQTLAEWVNRHQSPSYPRA"
     gene            5480..6313
                     /locus_tag="PLW25_05930"
     CDS             5480..6313
                     /locus_tag="PLW25_05930"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOS54062.1"
                     /translation="MKHSEGTFQGVRDLKIYHQAWLPEGKPRAAILIVHGLGEHSGRY
                     ANVVNRFVPSGFALYGIDHIGHGKSGGEREMLARFEDFTEPLAVYQKMIAEAHPGLPL
                     FIYGHSMGGLITAFYLLDHQKEFTGAILSAPAVKIPQNISALTVTLGRVFSTIAPKMG
                     LIQLDASGISRDDAVVKAYNNDPLVFHGKTTARLSAEMLRAMMRVTAEVEKITLPLFI
                     LQGSADYLVDPDAAKMVYEKAASVDKTLKLYDGLFHEVHNEPEREVMFNDLETWINAH
                     I"
     gene            6573..6917
                     /locus_tag="PLW25_05935"
     CDS             6573..6917
                     /locus_tag="PLW25_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOS54063.1"
                     /translation="MSHNSHLYIVWTTDNPITAMNMVTTYGGNSMKNGWWDEVTIILW
                     GASQQLVATDPSVAGRVRELMEMGVKFSACKRCADELRVTDALERLGVEVIYWGEPMT
                     RLLKSGATVLTI"
     gene            7211..7639
                     /locus_tag="PLW25_05940"
     CDS             7211..7639
                     /locus_tag="PLW25_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOS54064.1"
                     /translation="MNSDQAATLLKQKGVRPSYQRLRVLQYLHEHIGHPTAEEIFTAL
                     SVDIPTLSRTTIYNTLHTFVSYGLVNCLTLDGVETRYDVVLQKHGHFKCHRCGTIFNF
                     MMDVDRIETSGLEGFVIENKNIIFTGICPDCSFKKTKKGE"
     gene            7643..9112
                     /locus_tag="PLW25_05945"
     CDS             7643..9112
                     /locus_tag="PLW25_05945"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOS54065.1"
                     /translation="MSDKKKLTKLTTAAGAPVPENDNALTAGPRGPMLLQDVWYLEKL
                     AHFDREVIPERRMHAKGSGAFGKFTVTHDITKYTRAKIFSKVGKETECFVRFSTVAGE
                     RGAADAERDIRGFAIKFYTEEGNWDLVGNNTPVFFIRDPHKFPDLNRAVKRDPQTNLR
                     SATNNWDYWTSQPEALHQITITMSERGIPYSYRHMHGFGSHTFSMINDKNQRVWVKFH
                     LVTQQGIKNLTDAEAEAVIAKDRESHQRDLFDSIARGDYPRWKMFIQVMTEEQAKKMP
                     YNPFDLTKVWFKKEFPLIEVGVLELNRNPENYYQDVEQAAFNPAAVVPGIGFSPDKML
                     QGRLFSYGDAQRYRLGVNHHQIPVNNPKNAPAHASHRDGLMRVDGNHGATLGYTPNSF
                     GEWEAQPQYKEPPLELSGAADHWDARADDSDYYTQPGKLFRLMNAAQQKVLFENTARA
                     MGDAPEFIKIRHIGNCLKADPEYGKGVAKALGISMEKVK"
     gene            complement(9311..9913)
                     /locus_tag="PLW25_05950"
     CDS             complement(9311..9913)
                     /locus_tag="PLW25_05950"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOS54066.1"
                     /translation="MNAFLHITAEAAGFNWYAWVVLPLIIFVARIFDVTLGTLRIIFV
                     ARGKRNLAPILGFFEVLIWIVVISQLVQNLESATAYIGYAAGFAAGNYFGMWLEDKLA
                     LGTYILRLITAEESGELVRRLRAAGFGVTTVAGEGKTGTVQLIYTFVKRRDVEAVLSI
                     IHELKPNAFVSMEEVRSTEKGIFPGGMAAQARGSLLRKSK"
     gene            10069..10770
                     /locus_tag="PLW25_05955"
     CDS             10069..10770
                     /locus_tag="PLW25_05955"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOS54067.1"
                     /translation="MTAQQQFASIRKRLLDFHPSAFLLAAQLLQLILYAAFEGMISQR
                     SLINAAGLVVLLMVLWVVTSSARVQWVAWLIAAPALGLSLLSAIFATPALQAWSSLLE
                     AILYFFTAGSLIVYMMGDNYVTTDELFAVGATFTLLAWGFAHAYLVCQIWAPGSILIS
                     TAPGREATFLELLFLSFTNLSATGLGDIITVSAHTRVLAMLEQFLGVGYVAMVVSRLI
                     GLVNNTRKRHKSEER"
     gene            10855..11283
                     /locus_tag="PLW25_05960"
     CDS             10855..11283
                     /locus_tag="PLW25_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54068.1"
                     /translation="MKNALKEVDRIIYTVDSGPILPELQAHEVYTITPSNITLKRTGI
                     YSNTEVYEGEWAFSGDEALLQELFTIAKRKDCSAYSRVEPEDAPDGGHTIRLTVVYAD
                     ESECTLIYDPGVTYKGADELLDKLKEVLNNLKSHPSMAPK"
     gene            complement(11479..>12416)
                     /locus_tag="PLW25_05965"
     CDS             complement(11479..>12416)
                     /locus_tag="PLW25_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54069.1"
                     /translation="HNLFPGEPGFISQYWMDTNTSSVASEKRAYGGDNFSNNFFERPF
                     TSIEMLYRPDIDLVRVEISSDANFYYFNLHLYGKSPEQDTLAGNYGVEIDFNRDGRGD
                     LLIWTTADGNTAWNIDKIFVFRDSNNDVGGQRPMRSDAPGYSGNGYDLLVFSPDHLDD
                     PDAAWKRVSPQNPNVLQLAVKKSLFENRAVFMWNGWADAGVKDPARFDYNDAFTAAEA
                     GSPYAGNVDYPLKSLFLVDNTCRLAYGFTPTGLEVGACLIYTPTPTPSATPTQPVCDC
                     SSFRNYSYIDTKECCEYCGYRWAGTSEFPCYAPRP"
BASE COUNT         3169 a         3196 c         3187 g         2864 t
ORIGIN      
        1 tcaaaactgc gcgtgagggt ctcgtctcct gccaggcgca tgtggtagaa aggacgcaca
       61 ccaatcggcc agtccgtcag gaaaacaaaa tcgtgatcgt atttctcttt tacataactc
      121 gcgatttcgc gttcaccgcc gggatcaata tcacccttct tatccgcggg caagacatac
      181 ccgcgagatt ttaatatctc taccgcgtca cgcatcggaa tgcgcgggaa aggcacctgt
      241 ggaaccacca gctcgacgcc gaaggtctct ttaattgcct ctccgtggag ctcctttacc
      301 ctggcataaa catattgcag ccagttctcc tcgaacgcca tcacatcttc atgtgaatcg
      361 atccaggaaa tttccaggtc aacaccagta aactcggtca tatggcgggc agtaaaggaa
      421 ggatcagccc tgaacaccgg tcctatctca aagatgcgtt caaaaccagc cgccatggcc
      481 atctgtttgt aaaactgagg agattgcgcc aggtaggctt tgcgctcaaa ataatccagc
      541 gaaaagagtt ccgctccgct ttcgctgggt gtgcccatca attttggcga atggatttca
      601 ataaagtcat gcttcagcca gtattcacgc attgccattt ccatggtggt ttgcacctgg
      661 aaaagcaggt tattgtaggc acggcgcagg tccatataac gccagtccag ccggtaatcc
      721 aacgcgggta aggtttcggc aaatgggtcg aaaggcaatg ggctttcagc caggttttcg
      781 accaccagtg actccagctg cacctcgagc tgattcagtt taaccaccgg gttgtcgatg
      841 accttgccgg taatagtcag agcagtttca gcccccaagc ccgcaatgtt tgatgccagg
      901 gtttcatcat tgggtttcca gtgagccacc tgcaccaggc ctgtggggtc acgtaagatg
      961 agaaattgca tctttttctg gtctctcaaa gtctgcagcc agccctgcag ttttactgtc
     1021 tggtcgatct tcccccgcag gtctttaatc aatgtccgtt ccattgttcc tcacttacct
     1081 gataggatgt aaagcctaat tataagagcc attgagcaat gaatcaatcg ccgcgcgggg
     1141 cgaaagttca cgcgcaaata cttgctgtag caatctgtca aaaacctgag gtggggttaa
     1201 ctgttgccag cgtttcacca acccggtctg cagcaggtga taaacctcgc tgcgcaacct
     1261 gtcctgctca cgcttttccc gcaaaccagt atcaattaaa tattcgcgat gcctgagtat
     1321 ttcggcgatg acttcatcaa tcccattgtg ctgggtggct atcgtttgga tgataggagg
     1381 ggtccaggcg gtcgtatttc tgacttcatt ctctttaatt gtcatcctgt gagcgccatc
     1441 gtggttcatg tgtaggtgat agccaaggtc gaggttggca cgcaacgcgc gcgcggtatt
     1501 ctcagcgcct ggcaggtcgg atttgttgac caccagaata tcggcaatct cgaggatacc
     1561 agccttaatc gcctggatat catcgcccaa tcccggggat tccaccacca gggtggtgta
     1621 cgccaggcag gcgatatcca cctcactttg cccggctccc accgtttcga taaggatgaa
     1681 atcaaacccc gctgcgtcca gcacctgcac cacgccagcg gtggcgctcg ccaggccacc
     1741 cagctcgccg cgcgaggcca ttgaacggat aaacacaccc gggtcgcccg ccagttcgcg
     1801 catgcgtaca cggtcgccca atatcgcacc gccagtgaag gggctggaag ggtcaaccgc
     1861 cacaattgcg accgtttgtt tttgcgcaac acggatgttt tttgccaggc agctcaccaa
     1921 ggatgatttg cccgtcccgg gcgcaccggt gatgcccacc aggtgcgcct tgcctgcgcg
     1981 agcgaacaac cgatccaacg cctgttcacc ggcaggcgtc tggttttcca cctcggtcaa
     2041 caagcgggca agttcaaggc gattaccgcg ggtaaccgcg tccaggtcta ccatctcact
     2101 ccccgcaact cagacggctg aaaaggcgtc gcgaatatcc tgaacgactt tcgtggtcgg
     2161 tgttccaggt ccgtaaatgg cgctgacacc agcttgggtg agaacaggga tgtcttcttc
     2221 agggatgatg ccgccaacaa acaccttaac ctcttcctgc ccatttgcgc gtatcaggtc
     2281 gatcaccctg ggaacaagca cgaggtgcgc gcctgaaagg atagaaagac caataacatt
     2341 cacatcttcc tgcagggcag cctcggcgat catttcgggc gattgcctta acccggtata
     2401 aatgacctcc ataccggcgt ctctgagcgc gcgtgccacc accttcgctc cccggtcatg
     2461 tccgtccagc ccgggcttgg caatcaagac acgtatcttt ttttctgtca tagacatctc
     2521 cttggtgttt gcactggtac tcttccatct ctaattctac attcatcagc cgaaatctgc
     2581 tgtctttttt cgttattggg gcaaacttat atcctgtttt ggagaattta aaagttcaag
     2641 cgcgtgggag acagaaaggc caatcaaaaa agaacctcga gaaaaagaaa gtaagggaag
     2701 aagaaatcaa ctggctaccg agagaatctc aagcgacacg atagtggtgc gtaagaggtt
     2761 gcaagagaac gagctgaagg agttggaaga aaggagctgc tgaccacgtc tgatgcgcca
     2821 accgttattg tggagaacag tactgtttgg gcgagtgctg gagttacggg agcaattacc
     2881 gtgtaggaaa aaggataagc gggtcgtcct ggcacaacgt caagaggaaa gagttacagg
     2941 ctctactacg ggatgaatac gggcagacca gaggagaaaa agagaaaaag aaggccatat
     3001 gccatacgaa aaccaaaata tacaggtcaa tgaacctggt gacccggtac agatgcatac
     3061 tttatcgacc ttaataaata aggaacggtt ctataaccag atccgacctc atcaggcact
     3121 ggactatctc accccagccg agtatattaa taaatatcac cctgaagtaa cctcggaaaa
     3181 atctcatatg tattgaacgg gtacaatctc ttgaatcgtc tctccaaaag gttataatca
     3241 ttttggtgag tcatttccag tctccttgta tggtttagtc accttaagga tacttctgac
     3301 tcaccacttt tgttatagtt ctacattttc cagaaaggat gatgcatcaa cgttgaaaat
     3361 ccaattgaag tatttcatga gcccttaagg aggagaaatg gaatttcggg cgtatgatct
     3421 agttcggtca atggttgagc gcctgggggc acaatgacct atgagcgtaa aggttacgtt
     3481 cacggtgcct gggtgatcaa gatcgggcaa aatgatttgg tgatcaaagc gagtgagaac
     3541 agatcttttc cggaactgga tcagttatat actccacggg taaagaaccc gaagcattgg
     3601 gatgactatg cagagaaact cattccagat gcagaaaagc agttgtattc attgttagat
     3661 cttccatatt atccagttga aaagcgcgta aaactttctc aacttatcga gcgtacgaaa
     3721 tggaagttcg cctggaccta tgcccgcacc tatcctcacg aatacaccac aaaagccctt
     3781 tgtgaagctg ctgatcacgc gaagcttatt gattgtattg aaaagtatgg gatcattcaa
     3841 cgattcggcg agtcttaccg taagtatttc tactttgaag accgtaaata ctggcacatg
     3901 ggcgaaccat actctgacga cccggaattg cgaccaaatg tgataaaccg cacttgggtt
     3961 gatgtcaggc ggcatgctga aaatgtcaag catcgatgga ctgtggaaga ggttgaactt
     4021 cagcagcgat tatgggagat tcaactagaa aaatctacag accgaaacaa gccaaagaag
     4081 taaaaattaa tgcgataacc catttaattg cgctgtgtct cgaactcacc tcaccaaggt
     4141 tctcaagcac gcggatatga cgggctctga tggcgattca cccattctgc cagggtctgc
     4201 gcctggtaat ccatatgctt gtgcgagaag cgctgatcga tgacaagcga cagcatcctg
     4261 gaggccatat ccactaccgc cgtgttacca gaaacacgcg ggtcgcgtac gagcccctcc
     4321 cagtggatgg tcagaccgat accggcgtct cccagcgcgt cacccagttg gtctcgtgag
     4381 gggatgttga catacaccgg caggctcaag ggcgtgactc cctggggcag gatcggaaag
     4441 atcggggtga tccccggcag tccttctacc aaactcacga ggtagcgata attatcgcgc
     4501 cgttttttcc tgatgccatc ccaatcgagg tgctcgatgt gatggcgctc aacgatatcc
     4561 ccttccacca cctcgtctga ctcataatgg ttttcagcga gttcatagag ctgctggagt
     4621 tcattgcgat ctcccttgga gttgaaaaga taatcagcca ggcgtgagcg gtagtcccgg
     4681 atgaggggca atctcctgtt cttgagccca gccctttttt cgatataagg cagcaggtcg
     4741 aaccgcgagt ccagataccc gccatcatag gcgcacaact tgcgcatact gttgaaggca
     4801 aagtcgccac agacagttgg actgaatcca cattgcgcgt tatcctcgat cagcagggtg
     4861 ccgcccgcct ggagggagcg cagcaggtcg agctcagccg gctcacgggg acaaccgaaa
     4921 taatggatga agtagaccgc ccggtgaccc tccgcttttc gagccatctc ctcgaggtca
     4981 attgtcaggt caggctgcac cggataaaag tcgcacccca gcccgtgttt ttccagcgta
     5041 ttgaggatgg agggacaaag ataggagggc agcagtattt cattaattcc atgcgcgcgc
     5101 aggtaatcac caatcactat caagcaggct ttccccccgt tgaggaaggt caaatgggag
     5161 gtgtctacga cggaggtgtt cacctgccaa gaagaatcat aataaaattc tccacccaca
     5221 aacataatcc ctgctttttc aataaaaata aactaatctt ttctattgta tagtgattta
     5281 tacgggaatt aatgattttc gtttattgca ataatcgcaa gagcgggttg cgcccgggtt
     5341 tttcatttga aacgccaaag agcgctcgcc tgtagccacg agcagcaagt ggacgcgctt
     5401 gcgggtgaaa attcaatttg caaacctgtg atatagtgga agagtcagtt cgttagttca
     5461 tcaacaaagg gagagaaaaa tgaagcatag tgaaggcact ttccagggtg tgcgtgattt
     5521 aaagatatac catcaggcat ggttgcctga gggaaagccc agggcggcga tacttattgt
     5581 gcatggattg ggagaacaca gcggtcgtta tgccaacgtg gtaaaccgtt tcgtgccatc
     5641 tggtttcgcg ctctacggca tcgatcatat tggtcacgga aaatccggcg gagagcggga
     5701 aatgcttgcg cggtttgaag actttaccga accgttggcg gtatatcaga aaatgattgc
     5761 cgaagcccat ccgggtttac cgctttttat ttacggacac agcatgggcg ggttgatcac
     5821 cgctttttac ttgcttgacc accaaaaaga atttacagga gcgatcctct ccgcgcccgc
     5881 cgttaaaatc cctcagaata tttctgcatt gacggtgacc ctgggcaggg tattttcgac
     5941 gatcgctccc aaaatgggat tgattcaact ggacgcttcc ggcatctcgc gagatgacgc
     6001 ggttgtgaaa gcctacaaca atgaccccct cgtttttcat gggaaaacta ccgcgcggct
     6061 ttctgcggag atgctgcgcg ccatgatgcg ggtcacggct gaagtagaga aaatcaccct
     6121 gccattgttt attttgcagg gtagcgctga ttatctggtc gacccggacg cggcgaagat
     6181 ggtttacgag aaggctgctt cagtcgacaa aacgctcaag ttatatgatg ggctgttcca
     6241 cgaagttcac aacgaacccg agcgcgaggt aatgttcaac gacctcgaaa cctggataaa
     6301 cgcgcatatc tgatcaataa attaaataaa accaccggca gaagagggca ctctgccggg
     6361 ttgatttaat ggatgaatgg attggcacac acaacgttac ggcttaccag cagggagatc
     6421 acccgctgta gggctggttc accgccgctc tcagtgacga ggatgaaaag cgcgttggta
     6481 taatcgagcc atctgcgcgc cagcgaacag caccgctcac gagcataagg atagtgacga
     6541 aggcgcaacc aggtttgacc aaggagaggt aaatgagcca caactcgcat ctgtacattg
     6601 tctggacaac agacaaccca attaccgcca tgaacatggt gaccacgtat ggcggtaaca
     6661 gcatgaagaa tggctggtgg gatgaagtga ccatcatctt gtggggagca tctcagcaat
     6721 tggtcgccac ggatcccagc gtggcaggac gcgtgcgcga gttgatggaa atgggggtca
     6781 agttctccgc ttgcaaacgc tgcgcggatg aactgcgggt gactgacgcg ctcgagcgcc
     6841 tgggcgtaga ggtgatctat tggggggaac cgatgacgcg cttgctgaaa tccggcgcga
     6901 ctgtgctgac gatttaaagg tctacagggc agggggcaag gcaccctatt cttcaccatc
     6961 aagttgaaag aaagagaacc gcgggcttcg cgtatggccg aaatccggta tgtggagcat
     7021 tctaatacgc ttgtgacacg cgaaagatgg cggaaaatgg atgagaatcc atcgtgattc
     7081 gctaagaggc aacacccgct tcgcgctgcg ccaacctatc ctgttggtgt tttggcatcc
     7141 tttaatttat attgaaacaa aacctatatt gtaatcatta caataatgat gtataatcaa
     7201 ctaacagatg atgaattcag atcaagctgc tacactgctt aaacagaagg gcgtaagacc
     7261 ctcctaccag cgcttgcggg tgctgcagta tttacacgag catattggtc accctaccgc
     7321 tgaagaaata ttcaccgcgc tttcagtcga tatccccaca ctttcacgca ccaccattta
     7381 caataccctg cataccttcg tcagttacgg cctggtgaac tgcctgacac tggatggggt
     7441 ggaaacccgt tatgacgtgg tactgcaaaa acacgggcat tttaaatgtc accgctgcgg
     7501 aaccattttt aatttcatga tggacgtgga ccggatcgaa acgtcaggtc tcgaaggatt
     7561 cgtaattgaa aataaaaaca tcatctttac aggtatttgt ccggattgtt catttaaaaa
     7621 aaccaaaaaa ggagaataac agatgtcaga caagaaaaaa ttaaccaagc tcaccaccgc
     7681 agccggcgcg ccagtgccgg aaaatgacaa cgccttaacc gccggtccgc gcggccccat
     7741 gttgctgcaa gacgtgtggt atttggaaaa actggcccat tttgaccgtg aggtcatccc
     7801 agaacggcgt atgcacgcca aaggctcagg ggcgtttggc aagttcaccg tgacccacga
     7861 catcaccaaa tacacccgcg cgaaaatctt ctctaaggtt gggaaagaaa ccgagtgttt
     7921 tgtgcgtttc tcaaccgttg ccggagagcg cggcgcggcg gatgccgaac gtgatattcg
     7981 tgggtttgcc atcaagtttt acaccgaaga aggtaactgg gacctggtgg ggaacaatac
     8041 ccccgtcttc ttcatccgcg atccgcacaa gttccctgac ctcaatcgcg ctgtcaaacg
     8101 cgacccgcaa accaacctgc gctcagccac caataactgg gattactgga caagccagcc
     8161 ggaagcgctg caccagatca ccatcaccat gagcgaacgg ggcatcccat actcttaccg
     8221 ccacatgcat ggtttcggca gccatacttt cagcatgatc aacgacaaga atcaacgtgt
     8281 atgggtgaaa ttccacctgg tcacgcaaca gggcattaaa aacctcactg acgcagaagc
     8341 ggaagcggtc atcgctaaag accgtgaaag ccatcagcgc gacctgttcg acagcatcgc
     8401 caggggagat tacccccgct ggaagatgtt catccaggtg atgaccgaag agcaggcgaa
     8461 aaagatgccc tataacccct ttgacctcac caaagtttgg ttcaagaaag aatttccgct
     8521 tatcgaggta ggtgtccttg agctgaatcg caacccagag aactattacc aggatgtaga
     8581 gcaggcggct ttcaacccgg cagcggttgt ccccggcatc ggtttttctc ccgataagat
     8641 gctgcaaggt cgattgttct cctacggaga cgctcagcgt taccgactgg gggttaacca
     8701 tcaccagatc ccggtgaaca accccaagaa cgccccggca cacgcttctc atcgtgacgg
     8761 tttgatgcgc gtggatggca accatggcgc cacattgggt tataccccca acagcttcgg
     8821 tgaatgggaa gcccaaccgc agtacaagga accccctctc gaattgagcg gcgccgctga
     8881 ccattgggac gcccgcgcgg atgactcaga ttactatacc caaccaggca aactcttcag
     8941 gctgatgaac gccgcacagc agaaggttct ctttgagaac actgcccgcg ccatgggtga
     9001 cgcgcccgag ttcatcaaga tccgccacat tggcaactgc cttaaagcag acccggagta
     9061 cggcaagggt gtggccaagg ctttggggat ctccatggaa aaagttaagt aaaaagacct
     9121 gtaccgcctt cggggcagcc gtttggctgc cccggttttt taaaacctgg ttgctttatt
     9181 ccgtgatcgg gagacagatt taagataaag acagggcaag atatccaggt tgaccttgct
     9241 gagaggtaac ccttccgccg gctcacccgg gagagtcact ctgcctgagc cttgaagagt
     9301 gccgagggac ttatttcgat tttcgcagta atgacccgcg agcctgagcg gccataccgc
     9361 cagggaagat gcctttttct gtggagcgca cttcctccat tgaaacgaag gcgttgggtt
     9421 ttaattcatg gataatggaa agcaccgctt ccacatcgcg gcgcttgaca aaagtataaa
     9481 tcagctgaac cgtgcccgtt ttcccttcgc ctgcaaccgt ggtcaccccg aaccccgcgg
     9541 cgcgcaggcg ccgaaccagc tcccccgatt cttcagcggt gataagccgc aggatgtagg
     9601 tgccgagcgc cagtttgtcc tccagccaca tgccaaaata attaccggcg gcgaaacctg
     9661 cagcgtaacc gatataggct gtggcgcttt caaggttttg cactaactgg ctgatgacca
     9721 caatccagat gagcacctca aaaaaaccga ggatgggcgc aaggtttcgc ttaccgcgcg
     9781 ccacgaagat gatgcgcagc gtgcccaggg tgacatcaaa aatcctggcg acgaagatga
     9841 tcagcggcag caccacccag gcgtaccaat taaaacccgc ggcttcagca gtgatgtgta
     9901 agaacgcatt cataaaaata cctccacagg tacatctgtt cgtgaatcaa agcctgctgc
     9961 gagtataaca aagaaagctt tcaggtgcat ccattattga gatggtactg agctcacaaa
    10021 gatttttctc tctaccatca ataaaattcc actataattg agccaatcat gaccgctcag
    10081 caacaattcg catccattcg aaaacggctg ctcgattttc acccatccgc ctttctttta
    10141 gccgcccaat tacttcaatt gatcttatac gcggcctttg aaggcatgat aagccagcgc
    10201 tcattgatca acgcggcagg gttggtggtg ttgttgatgg tgttatgggt ggtcaccagc
    10261 agcgcgcggg tacagtgggt agcgtggctg attgccgctc cagccctggg tctttcactg
    10321 ctctcagcga tcttcgctac gccggctttg caggcctggt cttcgctgct ggaagcgatc
    10381 ttgtatttct ttacagcagg cagccttatt gtatacatga tgggtgacaa ttacgtaacc
    10441 accgatgagc ttttcgctgt tggggcaact ttcacactgc tggcgtgggg cttcgcgcat
    10501 gcttacctgg tgtgccagat atgggcaccg ggcagcatcc tcatcagcac cgcgccagga
    10561 agagaagcga catttttgga actgctcttc ttaagtttta cgaacttatc cgccaccggg
    10621 ctgggcgata taatcaccgt gtctgcgcac acacgggtat tggcgatgct ggagcaattc
    10681 ttaggggtgg gctatgtagc catggtagtt tcgcgtttga tcggtctggt gaacaacacg
    10741 cgcaaacgtc acaaatcgga ggaacgctga aaggtatcca tggtcattcg gctgaataaa
    10801 ttcacggtct gtttcgcatg cttgatcttt ttattttttt tcacggggtg tgaggtgaaa
    10861 aacgcgttaa aggaagtcga ccgcatcatt tacacggtgg attcggggcc catcctgccg
    10921 gaattacagg ctcacgaggt ttacaccatc actcccagca acatcactct gaaacgcaca
    10981 ggtatttatt cgaacacaga ggtgtatgag ggagaatggg ccttctcagg ggatgaagcg
    11041 cttttgcaag aattgttcac gatcgcaaag cgcaaggatt gctctgcgta tagccgcgtg
    11101 gaacccgaag atgcgcccga tggcgggcat accatcaggt tgacagtggt ctatgcagat
    11161 gaaagtgaat gcacgttgat ttatgaccct ggcgttactt ataaaggcgc ggatgaatta
    11221 ctcgacaagc tcaaggaagt gttgaacaac ttaaaaagcc atccgagcat ggcacccaaa
    11281 tagatagcgg gaggattttc atcgctaaaa atcctcccgc gttttgccga ttcgccctga
    11341 acaagatcag ggctatgccg tttgctgagg attatttcat gctctctgcc tgtgtgagcg
    11401 tgcagtaagc cctttgggtt cgaggggaca cctttcgctc atttccgagc gggacgccca
    11461 actgaacaag ctgatattct aaggtctagg tgcgtagcag ggaaattccg aagtgcctgc
    11521 ccagcgatac ccacagtact cgcagcattc cttggtatcg atataactat agttgcggaa
    11581 agaactacag tcacaaacgg gttgtgttgg cgtggcggag ggtgtaggtg tgggtgtata
    11641 tatcaggcaa gcgccaacct ccaggccggt gggcgtaaaa ccatacgcca gccggcaggt
    11701 attatcgacc aggaagagac ttttcagggg gtaatctaca tttccagcgt aaggagaacc
    11761 cgcctctgcg gctgtgaagg cgtcattgta atcaaacctg gcggggtcct taaccccagc
    11821 atcagcccag ccgttccaca tgaagaccgc ccggttttca aaaagcgatt ttttgaccgc
    11881 cagctgcaac acattggggt tttgcgggct tacccgtttc caggcggcgt ccgggtcatc
    11941 aaggtgatca ggcgaaaaga ccagcaggtc gtaaccgttc cctgaataac ccggggcgtc
    12001 agagcgcatc gggcgttgac cccccacatc attgttgcta tcccggaaaa caaaaatctt
    12061 atcaatattc caggcagtgt tgccgtccgc ggttgtccat ataagcaggt ccccgcgacc
    12121 gtcccggtta aaatctattt caaccccata gttgcctgcc agcgtgtctt gctcgggtga
    12181 tttaccgtaa aggtgcaggt tgaaatagta gaagttggcg tcagaactga tctccaccct
    12241 caccaaatca atatcggggc ggtacagcat ttcgatggaa gtgaacggcc tttcgaaaaa
    12301 gttattggaa aaattatctc cgccataagc gcgcttttcg ctggcaaccg aagaggtatt
    12361 ggtgtccatc cagtactggg aaataaaccc tggctccccg gggaacaggt tgtgga
//
LOCUS       DAOSPM010000053        12292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_5145_length_12292_cov_15.409823, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000053 DAOSPM010000000
VERSION     DAOSPM010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12292
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5145_length_12292_cov_15.409823"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="PLW25_05970"
     CDS             <1..1422
                     /locus_tag="PLW25_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOS54070.1"
                     /translation="LTPIAIRVMGGEFYGLWMILFAVGQLSLIGTLGIETIVNKFAAE
                     VKETYGADANLLSSAILVTLAMAFIVAAILFTFRGFLAQRIGPSPAHLTQFKNALAVY
                     SLMIIPQFTIRVFHGFFLAQIMNRFVRLMDLLNSLLPLLGGVILSLVAQNLTLLALWN
                     LFVSLIVLGVFLFRTYRLIGLRWLPTRVTTRRLLGFSPFMFIESAAINLFQQFDRLLV
                     ALMLGPAASGIYSVATGVGLRMSIIAGSVTEVLLPYASRKHSLGEKHKLENIVLKVTR
                     YISLMAALVGGLLIIWMKELLTVWISPQYAEANALFFCVIILAYSILSLSRPAHQTLV
                     GLGKVRFTSLVYLGSAALMLVTLCLLSMTRGLLGAGYANLVMTLLLCMSLYLFKWFED
                     DQVWRYFATDLILGMLPLVVALILVGMRCALPLKWVFTIGLLGLVFFVLARDDELKAR
                     LKRLVTGSALAKSSERGSQVKDK"
     gene            1490..2698
                     /locus_tag="PLW25_05975"
     CDS             1490..2698
                     /locus_tag="PLW25_05975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS54071.1"
                     /translation="MKLVFWGKYIDTYLADTLQRYEESTRMPVQYIVVEGEFTRRKVA
                     ATQAVKNRDQLTVLEKRKRQQQADRVLAANQDAVHLFLSFWGDKRLFGVLLKALHRGR
                     NVAVVFEPYASVPLGYWKDEARILSHFKILSRHIAYRFLWPLIKRAARGPLPCVLAVS
                     PLAQEQLRKVGFPEEVIFPFGYFTERKNGIPVQRKQAEVLRALFSGTLIRRKGLDIAI
                     AAVRRVNSPTVKVQLDIYGAGEVKKFLAHSEPGISYKGVYLQEQAQRVISAYDLMLVP
                     SRHEGWGLVVNEALMQSVPVCASDRVGAKCLLETSGAGLTFRSGDAAHLAALLAHLAA
                     DRHALQVMAQNCNRIANLITPSAGAEYLREVIQFYFAASGRKPKAFWLNASPENPSPD
                     MFSHRGKSGR"
     gene            2820..3881
                     /locus_tag="PLW25_05980"
     CDS             2820..3881
                     /locus_tag="PLW25_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54072.1"
                     /translation="MTRIAHILGGWSVNIGNAFYQLAGYQVLRECAPGAEITLIPEQP
                     GYPSYWNWKGGNPGNALEYVGRVAPDYLVLMGPVFRREIFNIWADALERLTRAGTRLV
                     LLGVGAMTYDAPHIHLYREFLSRFRPVLMVTRDRETYHLLGDLAEHAYDGIDFAFFLA
                     DYYPTCRIEGFSNTLALNFDKIPEPKIATEGSTSSPGASFHFAGMHWQVKFPRFRTWL
                     ARSSRYGMFLEGCLFGGRNELKVGGFDVIRLDHRPHPLLRAKTFRSPRTLVNDTPYPY
                     LDVYGNAALTLTDRVHAAVAALQYGKPAMLFSSSPRVRLLDRLDLAGIGEQPVWLDME
                     RLREEKTNIRDFLKANLKT"
     gene            4078..4800
                     /locus_tag="PLW25_05985"
     CDS             4078..4800
                     /locus_tag="PLW25_05985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOS54073.1"
                     /translation="MNSRISKLIALLRNPFYRRTLFRYRVAAAVEHDALLKRLGKDLR
                     TVVDIGANRGQFALVARFHSPEARIIAFEPLEEPAEVFQEMFRLDALMELHRCAVGAH
                     ASEAVMHLSKADDSSSLLPITALQTRVFPGTQERGTRRVTVRPLTDVLLPRQITPPSL
                     LKIDVQGYELQVLEGGVALLDCFDYLLVECSYAVFYKDQALAGEVLSYLEKVGFVLIQ
                     RCNLIYDQHGVPLQADFFFQRK"
     gene            4836..6059
                     /locus_tag="PLW25_05990"
     CDS             4836..6059
                     /locus_tag="PLW25_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS54074.1"
                     /translation="MTNSLGSETLRILLHDYGGYSFTNQLGRTLAGRGHRVLYSYSLT
                     TQLMQRFAPRPAHENLVMAGVQLPRTFNRYSYLRRWRDERLHGRLVGEQVRNFQPEVV
                     LTANTPLDAQVLIQRASREVKAAFLFWMQDLIGLAAQKNLSSRFPLIGPLLGAHYQQL
                     EKKLVIASDAVILISDSFFSYVRGWGITAERMHLLPNWAPLEEIPLLPKENPWAEAQG
                     LADKFVFLYSGVLGLKHDASLFLALAESFRSDPEVRVVIVAEGPFAEKLQAQACVSAL
                     ENLVVLPYQPPAEYPQVLASADVLMTILNTDAGAYSVPSKVNSYLCAGRPQLLSLPCD
                     NDAAQLVSSRKIGLVSEPGDVESWLMNARALYQNRYNHNQMAMYARAYAEENFDIERI
                     TDKCETMMKLALRGG"
     gene            6059..7531
                     /gene="wbaP"
                     /locus_tag="PLW25_05995"
     CDS             6059..7531
                     /gene="wbaP"
                     /locus_tag="PLW25_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HOS54075.1"
                     /translation="MAGSNGGQALAASAQTRFFDRHKRAFMFAIMLLVDAVALLLAAW
                     LAVLIRSLFSPELTDPALYYALFPYLLVFLLANFFTRLYPGNGITPDEELRGSTYAIS
                     AFMILLAIFLFLTQRGLEFSRFVFLLFWLLAVIFVPLLRLLAKYFGHRLGWWGEPIAI
                     LGFGEKGRTAYHFFKRNSLFGLNPLLIVHSSGNELREESDVRGVATLDEDMLAANPEL
                     VRRLGIRMAVIVPDEVSANLNSLLLDEQPFGLKRLVLISPFTWLGGPVVTAHHLDGLL
                     GLEVEANLLKPLQRAIKRCMDLALGVIGFICSLPLFAVIALLIKLDSPGPVLYRHERV
                     GKGGKPIRIWKFRTMVQGADALLAELVAQNADLRAEWEANHKLKQDPRVTRIGRWLRR
                     TSLDELPQVINILKGEMSLIGPRPIVREEVKFYQEGFALYQKVRPGLSGLWQVSGRSD
                     TSYAYRVSLDEYYIRHWTIWLDIFILLKTLWVVASRTGGY"
     gene            complement(7610..8143)
                     /locus_tag="PLW25_06000"
     CDS             complement(7610..8143)
                     /locus_tag="PLW25_06000"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOS54076.1"
                     /translation="MEQPAEGNGGSIDNRARKTLKPFLCRWLPVLFWAAVIFILSADS
                     NPYSHIPNRLYSWLWWSKLFGRSLIFYLGGISHILEYAILSFLFARALWQRERPTRGQ
                     FIKAISLVLFYAFTDELHQYFIPGRAFQLVDLALDTLGALLGLGAYWLYLERLEGKQS
                     RSTCALQGIRSRRKIGK"
     gene            complement(8107..>8624)
                     /locus_tag="PLW25_06005"
     CDS             complement(8107..>8624)
                     /locus_tag="PLW25_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54077.1"
                     /translation="NITDRQILEERLDINKMRCECIAMLRREFPQQFFGGIYPSDYAL
                     AVHGNYVIPDRRVFHKKAYLARMHDCDIGIATMGLFGSNGGKLGEYVAASKAIVSERL
                     RYQVPGDFSSDLNYLSFATPEECVAQVRRLVDDFDLRLAMMQRNHQYYSSYLRPDVLV
                     WNSLQKAMEAQ"
     assembly_gap    8625..8724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8763..9800)
                     /locus_tag="PLW25_06010"
     CDS             complement(8763..9800)
                     /locus_tag="PLW25_06010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS54078.1"
                     /translation="MSAAENKKVTLILPIRDEGPTIRAALDAVLAQSYPTDQMEILIA
                     DGMSTDGTREIVREYQRKHANIKLLDNPGRIVPTGMNTALRQAAGDIIIRVDGHCVIA
                     PDYVENCLKHLANDAVDGVGGPMKTIGLTPLAETIALAMSAPFGVGNSAFRTTQGQTK
                     YVDTIPFPAYKREVIQKAGLYDEELVRNQDDEYNYRIRELGGKLLLADDVRSVYYSRG
                     SMRGLWKQYYQYGFYKVRVLQKHPQQMSARQFIPLIFILSIFGSLALALFRNEGWVLL
                     ASILGAYLFAAILASFRISIKKGWKNFFLLPIAFITMHFSYGLGFAVGLIAFWKRWGD
                     KHGAVPSFTIE"
     gene            complement(9804..>10199)
                     /locus_tag="PLW25_06015"
     CDS             complement(9804..>10199)
                     /locus_tag="PLW25_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54079.1"
                     /translation="RYANHDLGYLPNWQHQCACERKLPSLASIAGRSNDLVLTKDGRE
                     LYILDSLYNGLPVIEGQLIQHTLDSFEVKVVPDETYSRSAMLGAITARLQQYLGAVNI
                     TLTEVTEIEKNANGKFKPFVSMINAQTYR"
     assembly_gap    10200..10299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10300..11359)
                     /locus_tag="PLW25_06020"
     CDS             complement(<10300..11359)
                     /locus_tag="PLW25_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS54080.1"
                     /translation="MNKQRVYQVLPSPLKNLAASVWGYYLRHLRFDHQTETLVEEALQ
                     RESWERNQWEEWQQVQLARLLQVAATTVPFYREQWQRRRRNGDKASWELLQNWPILDK
                     KEVRDNPRAFLSENSKSKHLYIDHTGGTTGKPTLIFESREVIRNIYALHEARTRRWFG
                     VHHRDRWGMLGGQKVIPLHQRRPPYWVWNGGLNQVYFSVFHINPETAKDYVRALWKFR
                     PVHLMVYPSSCAILSKFILEQRLRPPQIKVIVCNSENTLPKYRELMTRAFNCPVIDTY
                     GMAEACAAASECLALTQHYWPESGIIEVYDPQKREFSTEPGTAGEYVLTGLMNDDMPL
                     VRYANHDLGYLPNWQHQCA"
     gene            complement(11356..>12292)
                     /locus_tag="PLW25_06025"
     CDS             complement(11356..>12292)
                     /locus_tag="PLW25_06025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS54081.1"
                     /translation="YGVVTASGSPRRLKRLWDMVWTIISQRRAYQTAYVEVYNGSAFI
                     WAEIACGLLRLLKKPYCITIHGGRFIDFSRRHPRRVRRMIEKANIVTTPSSQFAHYFS
                     CWRSDILHLPNAIDTGFYPYRRRTLCQPKIGWLRKFDFPANPVLAAQVISLLKREFPE
                     IQLVMSGADLYNGAKQQTLEVIAQEGLSSNITITGFISRSQLQEWFKKPDIYLNTTNC
                     ESFGIAVVEAAACGLCIVTTNVGELPYIWEDGVDALLVPPNDPQAMAAAVKRILTDPE
                     LASRLSANAKKKAETYGWSVILTRWEALFNKMLKS"
BASE COUNT         2572 a         3129 c         3381 g         3010 t
ORIGIN      
        1 ctgacgccca tcgccatccg tgtgatgggc ggtgagtttt acggtctgtg gatgatcctt
       61 tttgcggtcg ggcagctttc gcttatcggc acgttaggaa tcgagactat tgtcaacaag
      121 ttcgccgcag aggtgaaaga aacctatgga gcggatgcca atcttctctc ttcagctata
      181 ttggttacgc tggcgatggc tttcattgtg gcagcgatcc tgtttacttt ccgtggcttc
      241 ctggcgcagc gcattggacc ctcacccgca cacctgaccc agtttaaaaa cgccttggcg
      301 gtatactctt taatgatcat cccgcagttc acgattcgag tttttcatgg atttttcctg
      361 gcgcaaatta tgaaccgttt tgtgcgccta atggacctgc tgaactcgct cttgccgtta
      421 ttaggagggg tgatcctctc gcttgtggcg caaaatttga ccctgctggc cttgtggaac
      481 ttatttgtca gcctgatagt gttgggtgtc tttctctttc gcacataccg tttgatcggt
      541 ttacgctggc tgcctactcg cgtaaccacc aggcgcctgt tgggattttc tccttttatg
      601 ttcattgagt ctgccgcgat caatctcttc cagcagtttg atcgtttatt ggtggcgtta
      661 atgctcggtc cagcggcatc cggaatttac agtgtcgcca ccggtgtggg gctgcgcatg
      721 tcgattatcg ctggcagtgt gaccgaagtg ctcttacctt acgccagccg caagcattca
      781 cttggtgaga agcataagtt agaaaatatt gtgcttaagg ttacgcgcta tatcagcctg
      841 atggctgcgc tggtgggtgg tttgctgatc atttggatga aagaattgct caccgtgtgg
      901 atctccccgc agtacgctga agccaatgca ttgttcttct gtgtgatcat actggcgtac
      961 agcatactca gtctcagccg cccggcgcac cagaccctgg tggggttggg caaggtcagg
     1021 ttcacctccc tggtttacct gggttctgcg gcactcatgc tggtaactct ctgcctgctt
     1081 tcaatgacca gggggttgct tggcgctggg tacgccaacc tggtgatgac acttttgtta
     1141 tgcatgagcc tttatttatt caagtggttt gaggatgatc aggtttggcg gtattttgcc
     1201 actgacctta tactgggtat gctgccgttg gtggtggcgc ttattcttgt cggtatgaga
     1261 tgcgctttgc cgcttaaatg ggttttcacc attgggctgt tggggttggt tttctttgtc
     1321 cttgccaggg acgatgagct gaaagcccgc ctgaaacgac tggtcacagg ttcagctttg
     1381 gccaagtcgt cggagcgtgg ttctcaggtg aaagacaaat gagcacccca ggcggagaac
     1441 gcgaaatggc gaagatctgt gagcatttca aggcgcagga gttggaaaag tgaagctggt
     1501 tttttggggt aaatatattg acacctacct ggctgataca ctgcagcgct acgaagaatc
     1561 taccaggatg ccggtgcaat atatcgtggt ggagggcgag ttcacccgcc gaaaggtggc
     1621 tgctacccag gctgtcaaaa atcgtgatca gttgacggtt ttagaaaaaa gaaaacgcca
     1681 acaacaggcc gaccgtgtcc ttgccgcaaa ccaagacgcg gtgcaccttt ttctcagctt
     1741 ttggggagac aagcggcttt ttggcgtgct gctgaaagcg ctgcatcgcg gacgaaacgt
     1801 ggcagtggtc ttcgagcctt acgcgagcgt tcccctgggt tattggaagg atgaagcgcg
     1861 catcctttct cattttaaaa tactgagccg acacatcgcc taccggttct tatggccgtt
     1921 gataaagcgc gctgctaggg gtccgctgcc ctgtgtattg gcggtttctc cactggcgca
     1981 agagcaattg cgcaaggtgg gtttcccgga ggaggtgatt ttcccattcg ggtattttac
     2041 cgagcgtaaa aatggcatac ccgttcaacg gaaacaggcg gaagttttgc gcgcgctgtt
     2101 tagcgggact ttgatcaggc gcaaggggct ggatatcgcc atcgccgcgg tgcggcgggt
     2161 taattccccc acggtcaagg ttcagctgga tatttatggc gcgggagaag taaagaaatt
     2221 tctcgcccac agcgaaccgg gaatctccta taagggcgtc taccttcagg aacaggccca
     2281 gcgcgtcatc agcgcttacg acttgatgct cgttccaagc cggcacgagg gctgggggct
     2341 tgtggtaaac gaagcgttga tgcagtcagt gccggtgtgc gccagcgacc gcgtgggcgc
     2401 gaagtgcctg ctggaaactt caggcgctgg cttaaccttc agaagcggtg acgcggctca
     2461 cctggctgca cttctcgctc acctggcagc ggaccgccac gcgctgcagg tgatggcgca
     2521 gaattgcaac aggattgcca acctgatcac tccttcagcc ggagcggaat acctcaggga
     2581 ggtcatccaa ttctattttg cagcttctgg ccgcaagccg aaggcttttt ggctgaacgc
     2641 ttcaccagaa aacccttcac cggacatgtt ctctcaccgt ggaaaaagcg ggaggtaaag
     2701 gaccaggcgg cttgacaagg tctcctcatt taacaatttc atctctcagc aaccaggggt
     2761 ttcgcggcga agtataatta acttgctttt atcatctcaa tcttgttgaa aaggaaaaaa
     2821 tgacgcggat tgcgcacatc ctggggggat ggtctgtcaa tatcggcaac gccttttatc
     2881 aactggcagg gtatcaggtg ctgcgtgaat gcgcccccgg agccgagatt acacttatcc
     2941 ctgagcagcc tggttacccg tcttactgga actggaaggg cggaaaccct ggtaacgcct
     3001 tggagtatgt tgggcgagtt gcgccagatt acctggtgtt gatgggacca gtttttcgcc
     3061 gcgagatttt caacatctgg gcagatgcgc tggaaagact cacgcgggct ggcacccggc
     3121 tggtgttact gggggttggc gcgatgacct acgacgctcc gcatatacac ctgtaccgcg
     3181 agttcctgtc gaggtttcgc cctgtattga tggtgacccg tgaccgcgaa acttaccatt
     3241 tgcttggaga cctggcagaa cacgcttatg acggcatcga ttttgctttt tttctcgccg
     3301 attattatcc gacctgcagg atcgagggtt tttccaatac cctcgccctc aatttcgata
     3361 agatccccga accgaaaatt gcgacagaag gcagcacttc ttcccccggt gcttcttttc
     3421 atttcgccgg tatgcactgg caggtgaagt ttccacgctt cagaacctgg ctggcgcgca
     3481 gttctcgtta cggaatgttc ttggaaggct gcctgttcgg cgggcgcaat gagttaaagg
     3541 tgggtggttt tgatgtgatc cgcttggacc atcgccctca ccccttgctg cgcgctaaaa
     3601 ccttccgttc gccgcgcaca ctggtgaacg acacgcccta cccgtacctg gacgtttacg
     3661 gaaatgccgc gctcaccctt acagaccgtg tgcacgctgc tgttgccgcg ctgcaatatg
     3721 gtaaaccggc gatgttgttt tcttcttcgc cgcgggtgcg cctgctggac cggctggact
     3781 tggcaggcat cggtgagcag cctgtatggc tggatatgga aaggctcaga gaagaaaaga
     3841 caaacatcag ggattttctt aaggcgaatc tcaaaacctg acctgctatg aaggtaattg
     3901 agaacagccc aagcgggtga tgatgcggca gaaccacgga tttgaataag gaaggcgagc
     3961 aatatgcctg caacgcggca ggagttatgg ataaggcgat aagcaggttt tcataagaaa
     4021 tataaccctg ctctgaggat gaatacgaac gaaatacgga caggtgagta ccggaaggtg
     4081 aattccagaa taagcaagtt gatcgctttg ctgcgcaatc ctttttaccg gaggacactg
     4141 ttcaggtatc gtgtggcggc ggcggtggag cacgacgcgc tgctgaaaag gctgggcaaa
     4201 gatttgcgca cggtggtgga tattggcgcc aatcgcggtc agtttgcgct tgtcgcccgt
     4261 tttcactcgc cagaagccag aattatcgcc tttgaaccgc tggaagagcc ggcggaggtc
     4321 tttcaagaga tgtttcggct ggatgctctg atggagcttc atcgttgcgc ggttggagcc
     4381 cacgccagtg aagcggtgat gcatctttcg aaagccgatg attcttcttc actgctgccg
     4441 atcacagccc tgcagaccag ggttttcccg ggaacgcagg aacgcggtac ccggcgggtg
     4501 acggttagac ccttgacgga cgtgctcttg ccacggcaga ttacacctcc atcgctatta
     4561 aaaattgatg tgcagggcta tgaacttcag gtcttagagg gcggcgttgc cctgcttgat
     4621 tgctttgatt acctcctggt cgaatgttct tatgcagtgt tttacaaaga ccaggcactg
     4681 gcaggcgagg tgctcagcta cctggaaaaa gttggttttg ttcttataca gcgctgcaac
     4741 cttatttatg atcaacatgg agtgccactt caggcggatt ttttctttca acgcaagtaa
     4801 gatacaatca ttcaaggaac agtgtcagga cgatattgac caacagtttg gggagcgaaa
     4861 cattgcgcat tttgctgcat gattatggcg gttacagttt tacaaaccag ttaggaagga
     4921 ctttggcggg tagagggcac cgggtactgt attcgtatag ccttacaacc caactgatgc
     4981 aacgttttgc accgcgaccc gcgcatgaaa acctggtaat ggcgggagta cagctacccc
     5041 gaacgttcaa tcgctacagt tacctgcggc gttggcggga tgaacggctg cacgggcgcc
     5101 tggtcggaga gcaggtgagg aatttccaac cggaggttgt gctcacggcc aacaccccgc
     5161 ttgacgcgca ggtgctgatt cagcgcgcca gcagggaagt gaaagcggcg tttctttttt
     5221 ggatgcagga tttgatcggg ctggccgctc aaaaaaacct ctcctcccgc tttccattga
     5281 tcggccctct gctcggagcg cattaccagc agcttgaaaa aaagttggtc attgccagtg
     5341 acgcggtgat tctcatttct gacagctttt tcagttacgt gcgcggttgg ggcatcacgg
     5401 ctgagcgtat gcatctactc cccaattggg cgccgctgga agagatcccc ctgctgccca
     5461 aagagaatcc ctgggcagaa gcgcaagggc tcgccgacaa attcgtcttt ctctacagcg
     5521 gtgtactggg gctgaaacat gatgccagcc tgtttctcgc cctggcggaa tctttccgct
     5581 ccgatcctga ggtgcgggtg gtcatcgtgg ctgaaggacc ttttgccgaa aaattacagg
     5641 cgcaggcctg tgtcagtgcc cttgagaacc tggtcgtgct tccctatcaa ccacctgcgg
     5701 aatatcccca ggtgctggca agcgccgatg tgctcatgac cattctcaat actgatgccg
     5761 gcgcctactc tgtgccttct aaagtcaact cgtacctttg cgccggtcga ccgcaactct
     5821 tgtcgctgcc ctgcgataat gacgcagccc aactggtttc ttcacggaaa atcggattgg
     5881 tctctgagcc gggagatgtt gagagctggt tgatgaacgc gcgcgcgctg tatcagaacc
     5941 ggtacaatca caaccagatg gcgatgtacg cgcgcgccta cgctgaagag aattttgata
     6001 ttgagcgcat taccgataaa tgcgaaacca tgatgaagtt agccttgaga ggtggttgat
     6061 ggcaggttca aatggagggc aggcattggc ggcatccgcg cagacccgct tttttgaccg
     6121 ccataaacgc gcgttcatgt tcgctatcat gctgctggtt gacgcggtag cgttgctgct
     6181 cgctgcgtgg ctggcagtgc tgatccgttc tctcttctcg ccagagctca ctgaccctgc
     6241 gctctattac gcgcttttcc cttacctgct cgtcttcttg ctggcgaatt ttttcacccg
     6301 cctttaccct ggcaacggta tcactcctga tgaggaattg cgcggctcca cttacgcgat
     6361 cagcgctttc atgatcctgc tggcaatatt tctttttctc acgcagcgcg ggttggaatt
     6421 ctcccgcttt gtgtttcttc ttttctggtt gctggcagtc atttttgtcc cgttgctgag
     6481 attgctggcg aaatactttg gccaccgcct gggttggtgg ggagagccca tcgccatcct
     6541 cggctttggc gagaagggac gcacagccta tcactttttc aaacgcaatt ccctgttcgg
     6601 gctgaaccct ctcttgatcg tccattcatc aggaaatgaa ctgcgggagg aatcggatgt
     6661 gagaggggtt gcaacactgg atgaggatat gttggctgcc aaccctgaac tggtgcgcag
     6721 attgggcatc cgtatggcgg tcattgttcc tgatgaagtt tcagcgaacc ttaactccct
     6781 gttgttggac gaacaaccgt tcgggctgaa acgcctggta ctgatctctc cctttacctg
     6841 gctggggggc ccggtagtga cggctcacca cctggacggc ctgcttggac tggaggtgga
     6901 agccaactta cttaaaccgc ttcagcgggc catcaaacgc tgtatggacc tggcgctggg
     6961 cgtgattggt tttatttgtt ctttgccgct ttttgcggta atcgcgctgc tcatcaaact
     7021 ggattctccc ggcccggtgc tgtaccgcca tgagcgcgtt ggcaaaggcg gcaagcccat
     7081 acgcatctgg aaattccgca caatggtgca gggcgctgac gccctgttgg ccgagcttgt
     7141 cgcccaaaac gctgacctgc gcgcggaatg ggaagccaat cacaaattaa agcaagaccc
     7201 gcgcgttacg cgcattgggc gctggttgcg ccgaacgagt ctggatgaac tacctcaggt
     7261 gatcaatatc ctcaaaggcg agatgagctt gattggtccg cggccgatcg tgcgggaaga
     7321 ggtgaagttc taccaggagg gtttcgcgct ctaccagaag gtgcgccccg gtttatcggg
     7381 gttgtggcag gtctcaggcc gctcagacac gagttatgct taccgcgttt cattggacga
     7441 atattatatt cgccactgga ccatctggct ggacattttc atcttgttga aaacgctgtg
     7501 ggtggtggca agcagaacgg gcggatattg aaatacacgc tgaaacagca aaacgcggcc
     7561 tatggccgcg tttttttgac tatacttcgg gtgagtttct acaactgtct cattttccta
     7621 tcttgcggcg tgagcggatg ccctgaagcg cgcaggtaga acggctttgt ttgccttcga
     7681 gccgctccag gtagagccag taggcgccaa gccccagcag tgcgccgaga gtatccaggg
     7741 ctaaatcgac caactggaac gctcgccccg gaatgaagta ctggtgcaac tcatctgtga
     7801 aggcgtaaaa cagaacgagg gagatggctt tgataaattg cccgcgagtg ggtctctcgc
     7861 gctgccagag cgcccgcgcg aataagaaag acagtatcgc gtattcgagt atgtgtgaga
     7921 tgccgccaag gtaaaaaatg agcgaacgcc cgaaaagttt gctccaccac agccagctgt
     7981 acagccggtt ggggatgtgg ctgtaagggt ttgaatcggc agagagaata aaaatcaccg
     8041 ccgcccagaa gagcacaggc agccagcggc agaggaaagg cttgagcgtt ttacgcgcgc
     8101 ggttgtctat tgagcctcca ttgccttctg caggctgttc caaaccagta catccgggcg
     8161 caggtaagac gaataatact ggtggttccg ctgcatcatt gctaatcgca ggtcaaaatc
     8221 gtccaccagc ctgcgtacct gggcgacgca ctcttcgggc gtagcgaaag aaaggtaatt
     8281 caagtcactg gaaaaatcac cgggcacctg gtaacgcagg cgttccgata caatcgcttt
     8341 cgaagcggcc acatattcgc ccagcttccc gccatttgaa ccaaacaacc ccatggttgc
     8401 aatgccgata tcgcaatcgt gcatgcgggc gagatatgcc ttcttgtgga agacgcgcct
     8461 gtcagggatc acgtagttac cgtgcactgc cagcgcgtaa tcgcttgggt agatgccgcc
     8521 gaaaaattgc tgagggaatt cccgccgaag catggcaatg cactcacagc gcattttatt
     8581 gatgtccaac cgctcttcga ggatctggcg atcggtgata ttttnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnaaaatc aacccaagac gattggcttt ttggtagcgc
     8761 acttactcaa ttgtaaaaga aggcaccgct ccgtgcttat ccccccagcg cttccaaaag
     8821 gctatcaagc ctaccgcgaa tcccaaacca taactgaaat gcatggtgat gaaggcgatt
     8881 ggcaggagaa agaaattttt ccatcctttt tttatggata ttcgaaagga agccaggatg
     8941 gcggcgaaga ggtacgcccc gagtatgctc gctaacaaga cccacccctc gtttcggaac
     9001 aaggcaagcg cgagcgaacc gaagatgctc aggataaaga tcaacgggat aaactgccgg
     9061 gcgctcatct gttgagggtg tttttgcaat actctcacct tgtagaaccc gtattgatag
     9121 tattgtttcc ataaacccct cattgatccc cggctgtagt atacagacct cacatcatcc
     9181 gccagtaaca gctttccgcc caactcacgg atgcgatagt tgtactcatc atcctggttg
     9241 cgcaccagtt cttcatcgta caacccggct ttttggatga cctctctctt ataagcgggg
     9301 aaggggatcg tgtcgacata tttcgtttgt ccctgggtgg tacggaaggc ggaattgccc
     9361 accccaaagg gggcgctcat ggcgagcgcg atggtttcag ccagcggggt cagcccgatg
     9421 gttttcattg ggccgcccac accgtccaca gcatcgttcg cgaggtgttt caggcaattc
     9481 tctacgtaat caggggcgat gacgcaatgc ccatccacgc ggatgatgat gtctcccgcg
     9541 gcctgcctta aagcggtgtt catgccggtc gggacgatcc tgccggggtt atccagtagt
     9601 ttgatgtttg cgtgcttgcg ctggtactcg cggacgatct cacgcgtgcc atctgtggac
     9661 atgccgtccg cgatgaggat ttccatctga tcggtcgggt aactttgcgc taaaacagca
     9721 tctaacgccg cgcgaatggt aggcccttca tcgcggatgg gaagaatgag ggtgaccttt
     9781 ttgttctccg cagcgctcat ctttcaccgg taagtttgcg cattgatcat ggaaacgaac
     9841 ggtttaaatt tcccattggc gttcttttca atctccgtca cttctgtgag ggtgatattc
     9901 accgcgccaa ggtactgctg caaacgcgcg gtgatggcgc ccagcatcgc gcttcggctg
     9961 taggtctcat ctggaactac tttcacttca aatgaatcca gggtgtgttg gatcaactgc
    10021 ccttcaatca ccggcagacc gttatacagg gaatcgagga tgtaaagttc acgaccgtct
    10081 ttggtcagca ccaggtcgtt gcttctcccg gcgatacttg ccagcgaagg cagtttgcgt
    10141 tcgcaggcgc attggtgctg ccagttgggt aagtagccaa ggtcgtgatt ggcgtaacgn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ggcgcattgg tgctgccagt
    10321 tgggtaagta gccaaggtcg tgattggcgt aacgtaccag cggcatgtcg tcattcatta
    10381 acccggtcag cacgtattcg cccgctgtgc caggctccgt gctgaattca cgtttttgag
    10441 gatcatacac ttcaataatg ccgctttccg gccagtaatg ttgcgtcaac gctaaacact
    10501 cgctggctgc cgcgcaggct tccgccatgc cgtaagtgtc aatgaccggg cagttgaacg
    10561 ccctggtcat taattcgcga tactttggca gggtgttttc tgagttgcaa acgatcacct
    10621 tgatctgtgg agggcgaagc ctttgctcaa ggatgaactt ggacaatatc gcgcaggagg
    10681 acgggtacac catcaggtga accgggcgga atttccacag ggcgcgcacg tagtctttcg
    10741 cggtttcagg gttgatgtgg aaaaccgaga aatagacctg gtttaacccg ccattccaca
    10801 cccagtaagg cgggcggcgc tggtgaagcg gaatgacctt ttgccctccc agcatccccc
    10861 agcggtcacg gtgatgaacg ccaaaccagc gccttgtgcg cgcttcgtgc agcgcgtaga
    10921 tgttcctgat cacctcgcgg ctttcgaaga tcagcgttgg cttgccagtg gtgccgccgg
    10981 tatggtcgat atacaggtgt ttcgattttg aattctctga aaggaaggcg cgcgggttgt
    11041 ctcgcacttc tttcttgtcc agtatgggcc agttttgcag cagctcccaa ctcgccttat
    11101 cgccatttct tcttctgcgc tgccactgct cgcgataaaa gggcacagtg gtggcggcca
    11161 cctgtaacag gcgcgccagt tgaacttgct gccattcctc ccactggttt cgctcccagg
    11221 attctctttg cagcgcttct tccactaacg tttctgtttg atgatcaaac ctgagatggc
    11281 gtaaatagta tccccaaaca ctcgccgcga ggttcttcag gggagatggc aacacctggt
    11341 aaacgcgttg tttgttcacg attttaacat tttattgaac agcgcttccc accgcgtgag
    11401 gatgacagac cagccgtatg tttcggcttt ctttttcgcg tttgcactca agcgagacgc
    11461 cagttctgga tcggtgagga tacgtttcac tgccgctgcc attgcttggg ggtcgttagg
    11521 cggaacgaga agggcatcga ccccatcttc ccagatatag ggtaattctc ccacattggt
    11581 ggtgacgatg caaagcccac aggccgcggc ttctacaacc gcgatcccaa agctttcaca
    11641 attggtggtg ttgaggtaga tatcaggttt tttaaaccat tcctgcagtt gcgaacggct
    11701 gatgaacccg gtgatggtga tgttggatga caacccctcc tgggcgatga cctccagcgt
    11761 ctgctgtttg gcgccgttgt aaaggtctgc cccgctcatg accagctgaa tttcggggaa
    11821 ttctcgtttg agaaggctga tgacctgggc cgccagtacc gggttggcgg gaaaatcgaa
    11881 ttttcgcaac cagccgattt ttggctggca gagcgtcctt ctgcggtagg ggtaaaaccc
    11941 ggtgtcgatc gcgtttggca ggtgcagaat atccgaacgc cagcaggaaa aataatgggc
    12001 gaactggctt gaaggggttg tgacaatgtt ggctttctca atcatgcgcc ttacccgccg
    12061 cgggtggcgc ctggagaaat cgatgaacct gcccccgtga atggtgatgc agtaaggttt
    12121 tttgagcaag cgtaatagac cgcaggcgat ttccgcccag atgaaagcgc ttccattata
    12181 gacttcaaca tacgcagttt gatacgcccg cctttggctg atgatggtcc acaccatatc
    12241 ccaaagccgc ttcaggcgcc tgggcgagcc tgaagcggtg accaccccat ac
//
LOCUS       DAOSPM010000054        12163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_5209_length_12163_cov_15.045425, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000054 DAOSPM010000000
VERSION     DAOSPM010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5209_length_12163_cov_15.045425"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1591
                     /locus_tag="PLW25_06030"
     CDS             239..1591
                     /locus_tag="PLW25_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS54082.1"
                     /translation="MAQNFKEALDYSDRWLEIIEKAEVSEAEGKQIIEESKKNFAEFF
                     NKGWLDYRKSVTEAGDWACVEWTGRGAIFKDVLGREYIDCLGGYGMMDHGWSHPDVVK
                     AVAAQLRRTPMPSQELIDPLRGVLARLMANITPGDIKYSFFCASGTEAVEGAIKLAKM
                     YSKKSAFIVATKAFHGKTMGSLSMLGKADYRVPPGLLYAGQVYHVPYGDAAAVERQLQ
                     ICDQVGIGVAAVMMEPIQGEAGAIVPPDDFWPRIRKATREHGVLLIADEVQTGMGRTG
                     TMWGVEHWGVEPDIIAVAKSLGGGVMPVSSFCSTEEIWQVMMEPNPFIHTTTTGGGAL
                     ACSAAIAAINVALRDDFPKQAREKGDYFMKALKVLEKKYTNVYDYICGKGLLIGQHFK
                     DAEVGYKVAAGLFKRGVLVAGTLTSAHTIRIEPPIVIEYAQIDEVLNRLEDTLKEVSV
                     "
     gene            1641..3296
                     /locus_tag="PLW25_06035"
     CDS             1641..3296
                     /locus_tag="PLW25_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOS54083.1"
                     /translation="MVLEGYQYAWLVFLFIALFFAVRIIVGVWAGRKVQDNTDYVVAG
                     RRLPIHMAAASIMATWFAAETLMGASETAYDYGFQGVVFDPFGASACLFLSGFLFVRL
                     MRRAKYLTVVDFFERRYNKLTVVLSVIAEMLTYFSWTAAQIVAGGAIAQGIFGLDPVW
                     GCVIVITIVAGYTMMGGMMADTLLDFFQMFLTAIGISLVFIFMLKAVGGFPGLIEGAG
                     STYVSQPFKLLPTKGEGYLGYTGPTGWMYWIAAWMSIGLGSVATQDLMQRSMAARNQA
                     TSVYGSYFAGVLYLFFGIMSPLVGIMAYQLNPGMEDTSYLLVHAAMGHMPAILTAIFI
                     AALVSALMSTSDSSLLAGASAVTENLIPLLTGKKLEGKKALWATRIMVLVNAALGLSI
                     ALWAHTIYELSVVAWTLLLVGLFTPFSFGMYWKKANSSGANAAFIGGFAVWITAAIFF
                     FKTGLGGQSTALVCGYAGEGAFSEPWWCAFWDAVYVASFVAFISAIILMVVVSLATQK
                     KDPAKQLVDYYGEPINMSPKLHLGILPIRNAFRKITEEEKENT"
     gene            3450..3989
                     /locus_tag="PLW25_06040"
     CDS             3450..3989
                     /locus_tag="PLW25_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54084.1"
                     /translation="MTTFNPEELKYYALIFGVILLVIVLYRKAIKKVNTRQEGKLKAQ
                     PGYKGAAARAPLEKPALTEEQFARIWKNLSYLILMAGLGNLYMAYTAVRSATQTTGLW
                     VWWIDAVFSTLAAAAAWMMWRKKEKLWVFIYFILLLVPIFLFMSIKGTPFKISALIHL
                     FPLVLLYFALKPIWAGMKQ"
     gene            4391..>4608
                     /locus_tag="PLW25_06045"
     CDS             4391..>4608
                     /locus_tag="PLW25_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54085.1"
                     /translation="MRILTPLQEDLLSRERSLLNDLRLKLLGYDASQEDVTSLGHSIS
                     QLDDLFLLVIVGEFNAGKSAFINALLGQ"
     assembly_gap    4609..4708
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4709..5877
                     /locus_tag="PLW25_06050"
     CDS             <4709..5877
                     /locus_tag="PLW25_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54086.1"
                     /translation="SELEQVVEFVQQNAQVLLNSTPQIFPVSSRLALRAKMGEPQLWQ
                     DSGFEALETYIHNSLDQAEQVRLKFLNPLGVGAHLTGKYYAKAEAQRQILQEDVSMLQ
                     NVERQHEVYRNDMRKDFDFRMADVENIFYEMEQRGDAFFEDRFRLVKVMDLLKKERMR
                     SDFTREVVADLPEQVETRVNALIDWLVGCDLQQWKAVTSYLADRQREHKEKLIGEGIN
                     SHFTYDRERLLDAIGREAEQVVAGFDKHAEAAAIAENAKNAVAASAAVEVGAIGLGTL
                     VTVLATTMSADVTGILAAGAVAALGLFIIPAKRRKAKSELHQKISNLRAELVDSLRQE
                     FTKEIDLSLKRIEEAISPYTRFVRSESAHNEKILDELKYSQLAINQMRSQIESW"
     gene            complement(5900..7735)
                     /locus_tag="PLW25_06055"
     CDS             complement(5900..7735)
                     /locus_tag="PLW25_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS54087.1"
                     /translation="MSFSIGPTSSGSGPRTLLEDFGSVGEQSGEVYNPRVVRRMLAYL
                     APYRWRMVAALLLTLVEAGLTLLTPYLIKVAIDQYIIPANRQGLARIALTLTISFILL
                     FAASSFQRYIVSWVGQNVLTTLRADLFTKLQYLQQAYHDRHIVGVTVSRVINDVAEIN
                     ELLSQGIITLLGDLVVLIGIIIVMLSMSPRLALLTFTVLPIMLAATWLFSRQARKAFR
                     KTRSSVAAVVGDLAMDINGIRAIQAFTREKASQERFEQVNLKNRDAYINAMNLSFIFL
                     PGIEFLGMLATVIVLWFGGHYVMGGAVTLGVMVAFLSYVNRFFQPIQELSRIYTTFQS
                     AMAGGEQVVELLDTPLEIIEKPGAPELLPQNGEIRVTGVSFRYNPDSAEVLHDIHMVI
                     PAGKTAALVGPTGAGKTTIANLIARFYDTTAGSITIDGVDIRDVTINSLRRQVCVISQ
                     DPFLFSRSIAENIRFGNPAATDEAVMQAARQANAHDFIDRLPDGYNTRVLEGGVNLSQ
                     GQRQLLSIARALLTNPAVLILDEATANIDSVTEALLQDAIARMLKGRTAMVIAHRLST
                     VRNADLVFVIAEGKIVEQGTHNSLLKKNGLYRQLYERQFMDEPGT"
     gene            complement(7732..9525)
                     /locus_tag="PLW25_06060"
     CDS             complement(7732..9525)
                     /locus_tag="PLW25_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS54088.1"
                     /translation="MNQQKNLTPNVPLPMPSAPLFQVLRRAYGYLRPYWKKTAGAYIA
                     LFAVLGFNTLIPQFIRWIIDNGIAGKQMSVLTWSVLALLAMTIIKGALNYFVGLLSET
                     ASQNVAFDLRNDIQRKLTQLSFSFHDQSETGELLSRAVQDVERVRFLTGRATLRIAEG
                     VLLLLITSIVLLVMDFKLGLLTLLTMPALVYQAVRFGSRFRPLSLQVQKQLAALTTAV
                     EQNLRGGRVVKAFAQEEAEIERFGITNNTWLQLSNQAARMQAFNVPMLFLIANLGMAI
                     IIFYGGRQYIEGIISMGTIVAFVSYVGNLIEPVRRLGMIIPAIAIAGAASERIFDVLD
                     FSSQVRDEPGAKAIKKINGRVRFEHVSFHYGRNQILKDISFDVQPGQVVALLGLTGSG
                     KSSIVNLIPRFYDPSAGRILVDEMDIRAMTLKSLRRQIGIVLQETTLFAATIRENIAF
                     GNEKATDEEVIAAASAAQAHEFIMQTSQGYQTRIGERGVTLSGGQKQRIAIARAILMN
                     PRILILDDATASVDTATEQLIHQALTRLIQGRTTFVIAHRLSTVRHADLILVLEGGTI
                     AARGTHEELLRSSSTYRAIYNQQLKKQEETG"
     gene            9672..10751
                     /locus_tag="PLW25_06065"
     CDS             9672..10751
                     /locus_tag="PLW25_06065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS54089.1"
                     /translation="MRIAIVHDALCVSGGAERMVLWLAKAFPQAPVYTSVYLQANTFE
                     GFKALQVNTLPFSRLVKSERQFKLLYPLWYALIQREDFSNFDAVLSSSTYLAKFIRPT
                     STVRHACYLYAPFRLLWKPESYTPESLPIRGPATSLVKWVTPLLRRWDLKRTREIPKL
                     ATTCRNMANEIHKVYNMQAQVIYPPVEIPPQMAIEKGGEYYLSVSRLISHKRVDLAVK
                     ACTRLGRQLLVVGDGPERARLEQMAGPTIRFVGRVSDEEVQRLFSRARGLIFPSYEDY
                     GIVPLEAQAWGVPVIALGKGGVLETVKENVSGVFFERQEEDSLVEGIKRFESSRFDAR
                     LIRDWAGEFNGERFIAQVRQFVQAP"
     gene            10812..>12163
                     /locus_tag="PLW25_06070"
     CDS             10812..>12163
                     /locus_tag="PLW25_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HOS54090.1"
                     /translation="MTQNTQNGITELALSNEYKPWLVSGLLLLSDLSAIILSFILSFY
                     LRKALIPIIGGVLDFNQIAPLFLLIILFILVIFLLFGLYPGHGRTGVVEFREIVYIVS
                     FSHIIVAMIIFILGYGPRFSRLVFLFNWCFALVLITSFRLWLHNRGSLRRWWGQPAVV
                     IGGSKDVTMVIRQLKSARRLAYRPIAAIVLDKDAPQEKIHEVPVYPFSEKQLNEFRKA
                     NIRLGIFTSYTVSTRLGFRKYLQTFSLVFPKLLYVLGDSPLNMLAMKTMDLDGHPALQ
                     VQYNLLNPFALFVKRAADLFLSLLSLVFTFPLFLLFSLIIWIDSPGPVLYKQTRMGKG
                     GKLFNIYKFRTMVVGADKKLQDLLAADENLRDEFERYHKLQDDPRVTRVGRFLRKTSF
                     DEFPQILNVIKGEMSWVGPRAYLPAELEKMGDSAAIIHRVTPGLTGWWQVMGRHSVSF
                     "
BASE COUNT         2792 a         2947 c         3366 g         2958 t
ORIGIN      
        1 ttaagattag aaaaaaggaa cgtgttccaa taggagatgc tctacggaaa attgttaata
       61 aaaaatactt gacacgatgt tttttcttgt cataataaat caataggaaa aacatccatt
      121 ttcccattga ctcctggttt gtcggtattc tccaacccgc acttgcacga aatcagcaat
      181 ttccctgctc tcttgttcta tttgacaccc aaaatagttg aagatcgaag gaggagagat
      241 ggcacagaat tttaaggaag cgcttgacta ttcagatcgc tggttggaaa tcatcgaaaa
      301 agccgaggtg agcgaagccg aaggcaaaca gatcatcgaa gaaagcaaga aaaatttcgc
      361 ggaatttttt aacaagggct ggttggatta tcgaaaatcc gttaccgaag caggcgattg
      421 ggcctgtgtg gaatggacag gcaggggggc gatttttaaa gatgtgttag gtcgtgaata
      481 cattgactgc ctgggcggtt acggcatgat ggaccatggg tggagccacc ctgatgtggt
      541 aaaggcggtg gcagcgcagt tgcgccgcac acccatgccc agccaggaat tgatcgaccc
      601 actgcgcggc gtgttggcca ggctgatggc caatatcacc ccgggcgata tcaagtattc
      661 tttcttttgc gccagcggca ctgaagcagt agaaggcgcg atcaaactgg ccaaaatgta
      721 ctcaaagaaa tcggccttta ttgttgcgac caaggctttc cacggcaaga ccatgggttc
      781 gctctccatg ctgggcaaag cagattaccg cgtaccgcct ggcttgctat acgccggtca
      841 ggtttaccat gttccatatg gcgacgcggc tgcggttgag cgccagcttc aaatttgcga
      901 ccaggtgggt atcggcgtgg cggctgtgat gatggaaccc atccagggtg aagccggcgc
      961 gatcgtaccc ccggatgact tctggccgcg aattcgaaaa gcgaccaggg agcatggtgt
     1021 gctgcttatt gccgatgaag tgcaaacggg catggggcgc actggaacca tgtggggtgt
     1081 ggagcactgg ggggtggaac ctgatatcat tgcggttgcc aaatctttgg gcggtggggt
     1141 catgccggta agctccttct gctctacaga agagatctgg caggtgatga tggagccgaa
     1201 tccgttcatt cataccacca ccactggcgg cggcgcgctg gcctgttcag cggctatcgc
     1261 cgccatcaat gtcgcgttgc gagatgattt tccgaagcag gcacgcgaaa aaggcgatta
     1321 ctttatgaag gctctcaagg tgctggagaa aaaatacacg aacgtatacg actacatctg
     1381 tggaaaagga ttgcttatcg ggcaacactt caaggatgcg gaagtcgggt acaaggtcgc
     1441 ggcagggctc tttaaacgcg gcgttctggt tgctggaacg ctaaccagcg cccataccat
     1501 tcgcattgaa ccgcctattg ttattgaata cgctcaaatt gatgaggttt tgaaccgcct
     1561 ggaagataca ttgaaagagg taagcgtctg aatgattgat acctcaactt atccgtctca
     1621 ggtcagaaag gaagggtcga atggttcttg aaggttatca atacgcttgg ctggtttttc
     1681 tgttcattgc gctcttcttc gccgtgcgca tcatcgtggg cgtgtgggcc ggtcgaaaag
     1741 ttcaagacaa tacagattat gtggtggcag gaagaagact gcccattcac atggctgcgg
     1801 cttccattat ggctacctgg ttcgcagctg aaaccttgat gggcgcttca gagacggctt
     1861 atgattacgg cttccagggg gtggtttttg acccctttgg cgcttcagcc tgtcttttcc
     1921 tttcaggttt cctttttgtc cgcctgatgc ggcgcgcgaa gtacctgacc gtggtggact
     1981 tctttgaaag acgctacaac aagctgacgg ttgttctctc ggtcattgcg gaaatgttga
     2041 cctacttctc ctggactgcc gcgcaaattg tggcgggtgg cgccatcgcc caaggtattt
     2101 ttgggcttga cccggtatgg gggtgcgtaa tcgttatcac gattgtggcc ggctatacca
     2161 tgatgggcgg catgatggcg gatacgctgc tggatttctt ccagatgttc ttaacggcta
     2221 ttggcatctc gctggtgttc atttttatgc ttaaagcggt cggcggcttc cccggattga
     2281 tcgaaggagc ggggtcgacg tatgtgtctc aaccctttaa actgctgccg accaagggtg
     2341 aaggttacct gggttatacc ggccccaccg gatggatgta ttggatagcc gcctggatgt
     2401 ctataggact tggctccgtg gccacacaag atctcatgca gcgttcgatg gccgcccgca
     2461 accaggcgac ttcggtttac ggttcgtatt tcgctggggt gctctatctg ttctttggca
     2521 tcatgagccc tctggtcggt attatggcct atcaactgaa tccaggcatg gaagacacca
     2581 gctacctgct ggtgcatgca gccatggggc atatgccagc aatcctgact gctattttta
     2641 ttgctgctct ggtttcggcg ttgatgagca cttctgatag ctcgctgctg gcgggggctt
     2701 cagctgtgac ggaaaacctg attccattgc tcaccggtaa gaagctggag gggaagaagg
     2761 cgctgtgggc aaccaggatc atggtgctcg tcaatgctgc actcgggctt tcaattgctt
     2821 tgtgggcgca tacgatctat gagctctccg ttgtggcctg gacgctgctg ttggtgggtc
     2881 ttttcactcc tttttccttt ggtatgtact ggaagaaagc caattcatcg ggggcgaacg
     2941 cggcttttat tggaggtttc gcggtctgga ttacagcggc tatcttcttc tttaaaactg
     3001 gtttaggagg gcaatccact gcgctcgtct gcggatacgc cggtgaaggt gcgttttctg
     3061 aaccctggtg gtgcgccttc tgggacgcgg tgtatgtggc gtctttcgtg gcattcatat
     3121 ccgcgatcat cctgatggtt gtggtttctc tcgcaacgca aaagaaggat cccgccaagc
     3181 aactggtaga ctactacggt gaaccgatca atatgagccc aaaactgcat ttgggtattt
     3241 tgcccatcag gaatgccttc cgcaagatta ccgaggagga aaaggaaaat acctgatttt
     3301 ccaatgcgtt acaagcggaa cgagatcatc agcaggctgc tcaactgaat ttgggtgtgt
     3361 ataatgaaag gcgctcaagg tcactggctt tgggcgccgc caaatccctg tgtggatact
     3421 tcctgtaatc ggagggtcag taggcaacca tgaccacgtt taatcctgaa gagctcaaat
     3481 attacgcgct tatttttgga gtcatcctgt tggtgattgt gctttatcga aaagcgatca
     3541 aaaaggtcaa tacaaggcaa gaaggcaaac tgaaggctca acccggttac aaaggggctg
     3601 cggccagggc gccgctggaa aaaccagccc tgaccgaaga gcagttcgct cgaatctgga
     3661 aaaatctctc ttacctgatt ttgatggctg gcctggggaa cctctatatg gcttacacag
     3721 cggtcaggag cgccactcaa acaactggat tatgggtgtg gtggattgac gcggttttca
     3781 gcaccctggc tgccgcagcc gcatggatga tgtggcgcaa gaaagaaaag ctttgggtat
     3841 ttatttattt tattttgctg cttgtcccca tcttcctgtt tatgtcgatc aagggcactc
     3901 ccttcaagat cagcgcgctc atccaccttt tccctttggt tctgctttat tttgcgctca
     3961 agccgatctg ggctggcatg aaacagtaat aatcaggcga accgataata acttcgcgta
     4021 aggaattgcc tgaaggacca gagtgttctt tttaccggtc acgcgcgggg cattattttg
     4081 tttgttttcg aatgacaatt tcgctaccag cttcgggggc acccgccgtg gtaagccccg
     4141 ggtgatggaa ggacagcaga aggattaaca acctccctgt gttcaggcgt atggggtaac
     4201 tcaatgagaa accaccttcg cgactctgtt ccaatctgcg gcggtttggt tcagggttgc
     4261 tcatttccac cctgttaaag gcgttggcaa taaggctata ataaccttgc ctgcacacaa
     4321 aggctggaat ggatatgtat tccagagcgg gcagcaacat gaaagctggt aaagagaagg
     4381 aaggagcgct atgagaatct tgacaccatt gcaggaagat ctactgtctc gtgagagaag
     4441 cctgctcaat gacctgcgtt taaagttatt gggttatgac gcctctcaag aagatgtgac
     4501 ctctctcggg cattccatct ctcaactcga tgatcttttt ctgctggtga ttgtgggcga
     4561 attcaacgct ggcaagagcg cgttcattaa cgcgctgtta ggtcaaaann nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa agcgaacttg aacaggtggt tgaatttgtt
     4741 caacaaaacg cccaggttct actgaacagc acgccgcaaa tctttccggt aagttccagg
     4801 ctggcgctgc gcgcaaagat gggagaaccc cagctctggc aggacagtgg ttttgaagcg
     4861 ttggaaacct acattcacaa cagtttggat caggccgaac aggtacgact caagttcctt
     4921 aacccgcttg gggtgggggc gcacctgacg ggtaaatact acgcgaaggc agaggctcaa
     4981 cggcaaatcc tccaggaaga tgtcagcatg ctgcaaaacg tggaaaggca gcatgaagtc
     5041 taccgcaatg atatgcgcaa agactttgat ttccgcatgg cggatgtgga aaacatcttt
     5101 tatgagatgg aacagcgcgg tgacgctttc tttgaagacc gtttcaggct ggtaaaggtg
     5161 atggacctgc ttaagaaaga acgcatgcgg tcagacttta cccgcgaggt ggtggctgac
     5221 ctgccagaac aggtagaaac cagggtgaac gcgctgatcg attggctggt gggatgtgat
     5281 ttgcagcaat ggaaggcggt tacttcttac ctcgcggacc ggcagcgcga gcacaaggag
     5341 aagctaatcg gtgaaggcat caacagccat ttcacgtatg accgggagcg gctgttagac
     5401 gccattggca gagaagcaga gcaggtggtg gctggttttg acaaacacgc cgaagcggcg
     5461 gccatcgcgg aaaacgccaa gaacgcggtg gcggcttctg cggcagttga ggtgggagcg
     5521 atcggtttgg gaacgctggt aactgttctg gctaccacca tgtccgcaga tgtaaccggc
     5581 atactggcgg ctggggccgt ggcggcgctg ggtctgttca tcatccccgc caaacgccgt
     5641 aaagccaaaa gcgagttgca tcaaaaaata tcgaacctgc gcgctgaact ggtagattct
     5701 ttaaggcagg agttcacaaa agagattgac ctcagcctca agcgcatcga agaagcgatt
     5761 tctccttaca cccgttttgt gcgctcggag agcgcgcaca atgagaagat cctggatgag
     5821 ttgaaatact cccaattagc catcaaccag atgcgcagcc agattgagag ttggtgagat
     5881 ttatccggat aagcgaattt caggttccag gttcatccat gaactgacgt tcgtacaatt
     5941 ggcgatataa gccattcttt ttcagcaggc tgttgtgtgt gccctgttca acgattttgc
     6001 cttcagcgat tacaaaaacc aggtccgcgt tgcgcacggt tgagagccgg tgcgcaatga
     6061 ccatggctgt gcgacctttg agcatgcgcg ctatggcgtc ttgtaacagg gcttctgtca
     6121 ctgaatcgat attggccgtg gcctcatcca ggatgagcac cgcgggattg gtcagcagcg
     6181 cgcgcgcgat gctgagcaac tggcgttgac cctgtgagag gttcacgccg ccttccagta
     6241 cgcgggtgtt gtagccatcc ggcaggcggt cgataaagtc atgcgcgtta gcctgcctgg
     6301 ccgcctgcat cactgcttca tccgtggcgg ctgggttgcc aaacctgatg ttctccgcga
     6361 tggaacgaga gaagagaaaa gggtcctgcg agatcacaca cacttgcctg cgcagggagt
     6421 tgatggtgac atcacggata tccacgccgt caatggttat agaacccgcc gtggtgtcat
     6481 aaaacctggc tatgaggttg gcgatggtgg ttttaccggc gccggttgga cctaccagcg
     6541 cagcggtttt gccggcaggg attaccatat ggatatcgtg aagcacttcc gctgaatcgg
     6601 gattgtacct gaatgaaaca ccggtcaccc gaatttctcc attttgcggg agcagttcgg
     6661 gcgcgcccgg cttttcgatg atttcaagcg gggtatccag cagctcaact acctgttcgc
     6721 cgccagccat cgccgactga aaggtggtgt aaatacggct gagctcctga atgggttgga
     6781 agaaccggtt gacgtaactt aaaaacgcta ccatcacccc caaagtgacc gcgccgccca
     6841 ttacataatg cccgccaaac catagcacaa tcactgttgc cagcattccg aggaactcaa
     6901 ttcccggcag gaaaataaac gataggttca tggcgttgat gtaagcgtcg cgatttttca
     6961 ggttaacttg ctcgaaccgt tcctgagagg ctttctcccg ggtgaaagcc tggatggcgc
     7021 gaatgccatt gatgtccatg gccaggtcac caacaaccgc agcgacactt gaacgggtct
     7081 tgcggaaggc tttacgcgcc tggcgagaaa acaaccacgt ggcagcgagc atgatgggta
     7141 gcacggtaaa tgtaagcagc gccaaccggg ggctcatgct cagcatgaca ataatgatgc
     7201 caatgagcac aaccagatca cccagcaggg tgatgatccc ttgagaaagc agttcattga
     7261 tctccgccac atcgttgatc acgcgagaca cggtcacgcc tacgatatgg cggtcgtgat
     7321 atgcctgttg cagatattga agttttgtaa agaggtcagc gcgtagcgtg gtgagcacat
     7381 tttgtccaac ccatgaaacg atataacgtt gaaacgagct ggcagcgaat aaaaggataa
     7441 acgaaatggt gagggtgagt gcaatgcgcg ctaacccctg gcggtttgcg ggaataatgt
     7501 actggtctat ggccaccttg atgaggtacg gtgtgagcaa ggtcagacca gcttccacca
     7561 gtgttaagag aagggcagcg accatacgcc agcggtaggg cgcgaggtaa gcgagcatgc
     7621 gccgtaccac gcgcgggttg taaacctcgc cgctctgctc accaacactg ccgaaatctt
     7681 ccagcagggt gcggggtcct gaaccagaac tggtgggtcc gatggaaaaa ctcacccggt
     7741 ttcctcctgt tttttcagct gctggttgta aatggcgcgg taagtgcttg agctgcgcag
     7801 cagttcctcg tgcgttccgc gcgctgcgat ggttccgcct tcgagcacca ggatgaggtc
     7861 agcgtggcgc acggtagaaa ggcggtgggc gatcacaaag gtggtgcgcc cctggatcag
     7921 cctggtgagc gcctggtgaa tgagctgctc ggtcgcggtg tctacagaag cggtggcgtc
     7981 atccaaaatg aggatgcgcg ggttcatcaa gatcgcccgg gcgatggcga tgcgctgttt
     8041 ctgcccgcca gaaagggtga cacccctttc gccaatccgt gtttgataac cctgagaagt
     8101 ttgcatgatg aactcatgcg cctgcgccgc gctggcggcg gcgatgacct cttcatctgt
     8161 ggctttctca ttgccaaagg cgatgttctc tcgaattgtg gcggcgaata atgtggtttc
     8221 ctgcagcacg atgccaattt ggcggcgtaa tgacttgagg gtcatggcgc ggatgtccat
     8281 ctcgtcaacc aggatgcgcc ctgcacttgg gtcataaaac cgcgggatta gattgacaat
     8341 ggaagatttt cccgaaccgg tgagccccaa taaagccacc acctgaccag gttgaacatc
     8401 gaaagagata tcttttaaga tttgattgcg cccgtaatga aaagaaacat gctcaaaacg
     8461 aactctcccg ttgatttttt ttatcgcttt ggcgccgggt tcatcgcgca cctgagagct
     8521 gaaatcgagc acatcaaaaa tgcgttcaga agctgcgccg gcgatcgcga tggcaggaat
     8581 gatcatgccc agccttcgca ccggttctat caggttgcca acgtaactga caaacgccac
     8641 gatggttccc atgctgataa tgccttctat gtattgcctg ccgccataga aaatgatgat
     8701 ggccattccc aggttggcaa tcaggaaaag cattggaacg ttaaacgcct gcatgcgcgc
     8761 cgcctggttg gacaactgga gccaggtgtt attggtaatg ccgaatcgtt caatttcggc
     8821 ttcttcttgt gcgaatgctt tgaccacccg accgccgcgc aggttctgct ctaccgcggt
     8881 ggtcaatgca gccagctgct tttgcacctg caatgagagc ggtcggaacc tggacccaaa
     8941 acgcacagcc tgatagacca gcgccggcat ggtgagcaag gtcagcaacc cgagtttaaa
     9001 atccattacc agcagcacaa tgctggtaat gagcagcagc agtacaccct cggcaattct
     9061 cagcgttgcc cggccggtga gaaagcgcac cctttctaca tcttgaaccg cgcgtgagag
     9121 cagctcgccg gtctcagact ggtcgtggaa ggagaaagac aactgggtca gtttccgctg
     9181 gatgtcattc cgcaggtcaa acgccacgtt ttgagaagca gtttcggata gaagccctac
     9241 aaaataatta agcgcccctt tgatgatggt cattgccagc aacgccagca cagaccaggt
     9301 taaaacgctc atttgtttgc cggcgatgcc attatcgatg atccagcgga tgaactgcgg
     9361 aataagtgta ttgaacccca acaccgcgaa aagggcgatg tatgctcccg cggtcttttt
     9421 ccagtagggg cgcaggtagc cgtaagcccg ccgcaacact tgaaagagcg gggcggaagg
     9481 catgggcagc ggtacgtttg gggtcaggtt cttttgctgg ttcaaagtga gccaccttgc
     9541 acagttttta gaatactaca atctattgaa aaattcaaag ccgacgcggt gggtgagtat
     9601 ttccacagct gtatcgttga ctggtttacg ttatacttgc ttaacccatt ttatgcgggc
     9661 aggagagctt gatgcgtatc gcgattgtac atgatgcatt atgcgtttca ggcggcgccg
     9721 agcggatggt gttatggctg gcaaaggctt ttcctcaggc gccggtttac acttctgtgt
     9781 acctgcaagc gaatacgttt gaaggtttta aagcgctgca ggtcaatacg ctacctttct
     9841 cccgcctggt taaatcagag cgccagttca agctgctgta cccgctgtgg tacgccttga
     9901 tccagcgcga ggatttcagc aacttcgatg ctgtgctgtc atcttctact tacctggcga
     9961 aattcattcg tccgacttcc accgtaagac acgcatgtta cctttacgct cctttccggc
    10021 tgttgtggaa gcctgaatct tacacccccg aaagcctgcc catacgcggc ccagccacct
    10081 ctttggtgaa atgggttact ccccttctgc ggcgctggga tttgaagcgg acgcgcgaaa
    10141 tccccaagct tgccaccacc tgtcgcaaca tggcgaatga gattcacaag gtttacaata
    10201 tgcaggcgca ggttatctac ccgcctgtgg aaattccccc gcaaatggcc attgaaaagg
    10261 gaggggagta ttatctttcg gtttcacgcc tgatctcgca caagcgggtg gatctggcgg
    10321 taaaagcctg tacccgttta gggagacaac tgctggtggt aggggatgga ccggaaagag
    10381 ctcgcctgga acaaatggct ggcccaacga ttcggtttgt cggcagagtg agcgatgaag
    10441 aggtgcagag gttattcagc cgcgcgcgcg ggttgatctt ccccagctat gaagattacg
    10501 gtatcgtgcc attggaagct caagcctggg gggtaccggt catcgctttg ggtaaagggg
    10561 gagtgctgga gacagtcaaa gaaaatgtca gtggtgtttt ctttgaacgc caggaagaag
    10621 acagcctggt tgaagggatc aagcgcttcg aaagcagccg ctttgacgcc cgcttaatcc
    10681 gtgattgggc tggggaattt aacggcgagc gttttatcgc ccaggttcgg cagttcgttc
    10741 aggcaccatg aacgcaacgg agcacttgtt ttttgagtaa aatactctct ataagcaggg
    10801 gaggaccttc tttgacccaa aatacgcaaa acggcattac tgaactcgct ctctcgaatg
    10861 aatacaagcc gtggttggtt tccgggctat tgttactttc tgacctgtcc gcaatcattt
    10921 tatcttttat cctttcgttc tatctcagga aggcgcttat ccccatcatc ggtggtgtcc
    10981 tcgattttaa ccagattgcc ccattgttct tgttaataat cctcttcatc ctggtgatct
    11041 ttcttctctt tggtctctac cctggtcacg gccgcacggg ggtggtcgag ttcagagaaa
    11101 ttgtgtatat cgtctccttc tctcatatta tcgtggcgat gatcatcttc atcctcggtt
    11161 acggtcctcg gttttcgagg ttggttttcc tgttcaactg gtgctttgcg ctggtgttga
    11221 tcaccagctt ccgcttatgg ctccataacc gcgggtcatt gcgccgctgg tggggtcaac
    11281 ccgctgtggt tatcggcggc agcaaagatg tgaccatggt catccggcag ttgaaatcag
    11341 cgcggcgcct tgcctaccgc ccgattgcgg ctatcgttct cgacaaagac gccccgcagg
    11401 aaaaaattca cgaggtgccg gtctatccgt tctctgaaaa acaactaaat gaattccgta
    11461 aggcgaatat tcgcctgggg attttcacca gctacacggt ttcaacccgc ctgggttttc
    11521 gtaaatattt acagaccttc agcctggttt ttcccaaact gctgtatgtt ttaggagatt
    11581 cacctttgaa catgctggca atgaaaacca tggatttaga cgggcatcct gcactgcagg
    11641 tgcagtacaa cttattgaat ccttttgcat tgtttgttaa acgcgcggcg gatctgtttt
    11701 taagcctgct cagcctggtc ttcacctttc cgcttttcct gctcttttct ctcattatct
    11761 ggatagactc acctggaccg gtgctgtata aacagacacg catgggcaaa gggggaaaac
    11821 tattcaatat ttataagttc cgcacgatgg ttgtgggggc ggataaaaaa ctgcaggatt
    11881 tgctggctgc ggatgaaaac ctgcgcgatg aatttgagag gtatcataaa cttcaggatg
    11941 acccgcgcgt caccagggtg ggcagattcc tgcgcaagac cagctttgat gaattcccgc
    12001 aaattcttaa tgtgatcaag ggagaaatga gctgggttgg accacgcgcc tatttgcctg
    12061 cggagctgga gaagatggga gattctgccg cgattatcca tcgtgtcacg ccagggctta
    12121 ccggctggtg gcaggtgatg gggcggcaca gcgtgagttt caa
//
LOCUS       DAOSPM010000055        11563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_5561_length_11563_cov_14.102450, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000055 DAOSPM010000000
VERSION     DAOSPM010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5561_length_11563_cov_14.102450"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1324
                     /locus_tag="PLW25_06075"
     CDS             263..1324
                     /locus_tag="PLW25_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HOS54091.1"
                     /translation="MFKKSYLLVFVAILMVVSLMAGCAKPPVAPTTAPAEVEAPEAPA
                     AEESGVIKVYGAFATPIEEPWDGVIHDALLKAKEEGLIEYTYTEDIGYSGDMERVLRE
                     IAEQQKPQLIMGDAFVNEEAVRRVAADYPEIAFAFGSELGPVAPNLAVFDNWIHEPAY
                     LCGMLAGGLTKTNTIGVVAAVPIPEVNRLVNAFIAGAKETNPEVKVLVSFINSFFDPA
                     TAKEQALAHISAGADVLFAERAGVIEAAAEKGVFVFGNMSDQNEIAPEWVISGPVWNM
                     TPTVEYVVKQVKAGTFTAMDLKDFNMYALGGATLAPFHGNESKIPADVLAAVTAKEEA
                     IKSGMFRVPIDEAQPAAVN"
     gene            1405..2889
                     /locus_tag="PLW25_06080"
     CDS             1405..2889
                     /locus_tag="PLW25_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS54092.1"
                     /translation="MLAVEMKHINKTFGPVKANQDVSFELEQGEIHALLGENGAGKTT
                     LMRILYGLYRADSGEIVINGKKVTIHSPKDAIAAGIGMVTQHFTLVPTLTVAENIVLG
                     YSNKMVLSQKEIEKDVAQASEKYGIPVDPSTLVKNLSVGERQRVEILKALYRNARILI
                     MDEPTAVLVPQEVDVLFESLVRLVKSGLSVIFISHKLVEVMTVCDRITVLRDGKVANT
                     VKKSETTQVDLAKMMVGRETFGVQRQSAREASEPVYEVTEITLEGKHGRKVLDKVTFN
                     VGVGEILGIAGVSGNGQGELTEVLNGLVKPSSGTIKINGKDITCCSPSAISDCGIGRT
                     PQDRHEGTIGELTVAENLALEHISNYHRKGMLDRKKIQEDAAQTIQEFQIKASPGDQL
                     RKLSGGNMQKVVLARALSRKPKVLIASQPTRGLDVGATEYVRNRLLKERERGTAVVLL
                     SEDLDEVLALSDRIAVIYEGSIRGIIPAVEATPEKLGLLMAGAA"
     gene            2918..3970
                     /locus_tag="PLW25_06085"
     CDS             2918..3970
                     /locus_tag="PLW25_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS54093.1"
                     /translation="MIKFRLERSPAPVWTKALIPVVAVLVTFAITSIFILLAGANPFA
                     AYYNFIIVPLSTKFSALEVLVKATPLIFTGIAVTFAFAAGYYNIGAEGQLYAGAVAAA
                     WVGATFTTLPSIVVIPMMLVLGFLSGMLWALIPALLKARLKVDEVVTTLLMNSIIMYF
                     ISALLNGPWRDPETGWPKSPEFFEAARFMQLIPKSRLHIGFIIALVVVVVVYIIIKRT
                     PFGLQMRAVGASKAGAQFAGINVNRTMLIAALVSGGIAGLAGVSEIAGIHYHLISELS
                     GGYGYTGIIVATLGGLNPIGATIAALFIGLINTGAQTVSRVLGVPIYLGNVVQSTLLL
                     VTLGMLLLQNYRIKRI"
     gene            3973..4884
                     /locus_tag="PLW25_06090"
     CDS             3973..4884
                     /locus_tag="PLW25_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS54094.1"
                     /translation="MGDFLVDLIAATLRIATPLIFATLGELFCERAGILNLGVEGTMF
                     FGAFSGFTAASLTGSLWMGILCAFIGGMISGLIMSLFSVRLGVNQHVSGLGVTLLMTA
                     LSLFGFRVIFGENRILPSIQPFQQLSIFSGSRVLGPIFSQYTLTYIALALIPIVWWVV
                     FRTNFGLNLRSVGDNPEATDAAGINVYRLRTIALVIGGALMSVGGAYLSVAQLGSFTF
                     GIVAGRGWVCIALIIFANWHPVRVLWGALLFGGVQALQSRLQLTGLKLPYELFLALPY
                     IVTIIALTIAGRNASYPAALLKPYKRE"
     gene            5072..6634
                     /locus_tag="PLW25_06095"
     CDS             5072..6634
                     /locus_tag="PLW25_06095"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOS54095.1"
                     /translation="MAQDSTDKIAKLKALREESRLGGGEKRIAAQHEKGKKTARERID
                     ALLDKGSFNEINGLMKNRHTDFGLDKDKIPGDGLVAGFGTINGRKVCVYAQDFTVMGG
                     SFGEVAGEKVARVMDLAMDAGVPVIGINDGGGARIQEGIYSLYAYGEVFYRNVQASGV
                     VPQISVIMGPCAGGGVYSPALQDFIIMTQGMGNMYITGPEVIKAVTNEIVDHETLGGA
                     VTHTTVSGVAHFLGQDEDDTFHIIRKILEYIPDNNASTPPVLPTNDSPFRADKELNTL
                     VPADPNQTYDIRDVINRVVDIDSFFEVHHNFATNAVVGFARLNGESVGIAANQPASMA
                     GVLDINSSDKIARFVRFCDAFNIPVITFVDTPGFMPGTHTEHNGIIRHGAKILFAFSE
                     ASVPKISVVTRKAYGGAYIVMGSKHLRADMSFAWPGAEIAVMGAGGAVNILHGRSLKD
                     KDPEEAKKLRAELVADYEDKFSNPYQAASAGYIDEVIIPSETRQRLIATLDILRNKQS
                     TSPAKKHGNIPL"
     gene            6652..7008
                     /locus_tag="PLW25_06100"
     CDS             6652..7008
                     /locus_tag="PLW25_06100"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOS54096.1"
                     /translation="MENLGLALKISGIGIVVLLLVLAILAGIIYLLTLVLVDKEDEEE
                     SKDTVVDATTEPEKEKADSKSHALLAAALAVAIARAKAESVVSFADASTGEVNPWRQF
                     GLQRRLTQSSTIRRTR"
     gene            7005..7442
                     /locus_tag="PLW25_06105"
     CDS             7005..7442
                     /locus_tag="PLW25_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54097.1"
                     /translation="MTQYKITVKDTTYDVEILDDPRNDEVLVKVNGEELTVVTEEVNK
                     AVSAVKAAKPAPARAAAPAPKPAAAAPVAAGPGTIKSPLPGVVNSIKVRAGDKVKAND
                     EICVIEAMKAMNIIRAPQDGTVARIHVNEGNQVAYGAPLMDIE"
     gene            7460..8605
                     /locus_tag="PLW25_06110"
     CDS             7460..8605
                     /locus_tag="PLW25_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOS54098.1"
                     /translation="MGFDLSGLLGGLLDITWQQLIMIAVALLLLYLGIFKDYEPTLLI
                     PIGFGCILGNLPVAANMIGEEGLFGVLYRAGISNELFPLLIFLGVGAMLDLRPLLSQP
                     VMFVMGALAHLGIFATLVIAILLGFNLAEATAIGMIGAIDGPTVIYVNSKLGSLFVTP
                     GLAAAIAVTAYSYMSLVPIIQPPLMRLFTTKKERAIRMEYTPRPVSRMTVILFPILIT
                     AVAGIFLPEAAPLIATLMLGNLIKESEVVPGLVETSKNAITNTATIFLGITIGSTMQA
                     ATFLNTGTLRVLILGLIAFALNTIGGLLFGKLICLLSGRKINPLVGAAAISAFPMSGR
                     LAQKVALEVDNQNFILMHALGANAAGQVASVLAAGILMQMIFPILLH"
     gene            8741..9622
                     /locus_tag="PLW25_06115"
     CDS             8741..9622
                     /locus_tag="PLW25_06115"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOS54099.1"
                     /translation="MKLLDLTIPIGIGTPPWPTYEPLQVKYFKRLAPNGANGQVLTHS
                     NHLGTHLDGEIHFYTPGRDIAQLPMDFLVHEAAIVDLSDVCGDYDVYTSKMITDRVEV
                     KEGDILVIHTGYHHFGWDQPTADEVRYMTKHPGPDHEFVDWAIKMKLRWIAVDCGSAD
                     HPMNTIIRTWMPRQAKQAEYVFKKKFGKTLEEFFTDDKYQMMHIDLFPKEIIHAECFG
                     GMIDLLLNRRVEVGFFPWRFVDGEASIGRAVAFVDDKEYDELMKKAATMPKTKFGDVY
                     DPKHVESLNRLSKANLA"
     gene            9646..10371
                     /locus_tag="PLW25_06120"
     CDS             9646..10371
                     /locus_tag="PLW25_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54100.1"
                     /translation="MKFELPEHASQEVLEIDVREIEALLQGPAMDLPKWPGAQVKLKD
                     GRTLYIREARREEAPRMMEYMKRLMDVDHDFYDIVGARVYSEILGWYRHRLKDPYTLV
                     GLIDGVWAGFANGRVMNEKVNVSLHTMALLRGGRIGAVMYYAKAYYAFEMIGNTEWWA
                     TYESYNGWLRWGLGMAQPSYPWPEVQHELGGARVYYITKKYWDQLVKAYVQQMAGADL
                     DFNVPDAVRQANEEMIVPEEILV"
     gene            complement(10431..11315)
                     /locus_tag="PLW25_06125"
     CDS             complement(10431..11315)
                     /locus_tag="PLW25_06125"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HOS54101.1"
                     /translation="METIKVRLIGAAAQESLMKAAARRWVAGVTSKGAFLRTDDRVLF
                     LTNSDYRSPFNLTLNSIGQLFTRLQPGDTFETNKNEIVFSGREITLLTHGAAVWHPPQ
                     PARMVASLQEQRLRANLLLDTIKSIDPDKGFLFLAGQPDDQTEVFQREIHHAARQIVL
                     AYRSQNKEGFFASAEKILGAGGGLTPSGDDFLTGFLLYHYRYLQSVNSEPGLLNEWWQ
                     ELTRLAFKKTTTISANRLEYAGRGWAEEIFLTLIDHLFTPEVAFGIEKARHLVDFGHS
                     SGVDTLIGISYGLDSLLV"
     gene            complement(11389..11464)
                     /locus_tag="PLW25_06130"
     tRNA            complement(11389..11464)
                     /locus_tag="PLW25_06130"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11429..11431),aa:Phe,seq:gaa)
BASE COUNT         2770 a         3105 c         2973 g         2715 t
ORIGIN      
        1 ggtggtcctt gaccagccca acgtgcttga agcgctgcgc taccatgaaa aaccggtgta
       61 tgtgatcagc catggcaagc ttattgacca ggcgaagatg gacaagatca ttcaggaaaa
      121 ttcgtagccc gctatcagtt gctatgaagc acgcgattat gctatcctaa taaaagtgag
      181 aaaggaggaa gagccctggt tttaccaata cgaatagttg aaaacgcaag tgtatttaaa
      241 actagcaaag aaggagaaaa aaatgtttaa aaagtcatac ttgcttgtgt tcgtcgcaat
      301 tctgatggtc gtcagcctga tggctggttg cgccaagccg cccgttgcgc caacgaccgc
      361 ccccgctgaa gtcgaagcac ctgaagcccc cgcagctgaa gaatccgggg taatcaaggt
      421 ctatggcgcg tttgccaccc ccattgaaga gccatgggat ggtgtcatcc atgacgcgct
      481 gctcaaagcc aaggaagagg gccttatcga gtacacctac actgaagata tcggctactc
      541 aggcgatatg gaacgcgtgc tgcgcgaaat cgcagaacag cagaaacccc agttgatcat
      601 gggtgacgcg ttcgtgaacg aagaagccgt gcgccgtgtg gctgccgatt atcccgagat
      661 cgcctttgct ttcggttctg agcttggccc ggtcgctcca aacctggcag tatttgataa
      721 ctggatccac gaaccggctt acctgtgcgg tatgctcgcc ggcgggttga ccaagaccaa
      781 caccatcggt gtggtcgcgg ctgtgcccat ccccgaagtt aaccgtctgg tgaatgcttt
      841 catcgcggga gccaaggaaa ccaacccaga ggttaaggtt ctcgtttcct tcattaactc
      901 gttctttgat cctgccactg ccaaagagca ggccctggcg cacatcagcg ctggcgcgga
      961 tgtgctcttt gctgagcgcg ctggagtgat cgaagcggcc gccgaaaagg gtgtcttcgt
     1021 gttcggcaac atgagcgacc agaacgaaat cgcccccgaa tgggtcatca gcggtccagt
     1081 ctggaatatg acccccaccg ttgaatacgt ggtcaagcag gtcaaggctg gcactttcac
     1141 cgccatggac ctgaaggact tcaacatgta tgcgttgggc ggcgcgacct tggcaccatt
     1201 ccacggcaat gaaagcaaga tcccggccga tgtgctcgcc gcggtcactg ccaaagaaga
     1261 agccatcaag tctggtatgt tccgtgttcc catcgacgaa gcacaaccag ccgctgtaaa
     1321 ttaatccgaa aatcaggggc ggcagaaggt aactctgccg ccccccttta aatccgtacc
     1381 gaaatctacg atgaaggatt tgccatgtta gctgttgaaa tgaagcacat aaataaaacc
     1441 ttcggcccgg taaaagccaa tcaagacgtg agtttcgaat tggagcaggg tgagattcac
     1501 gccctgttag gagaaaacgg cgccggtaaa acaaccttga tgcgtattct ctatggttta
     1561 tacagagccg atagcggtga aatcgtcatt aatggtaaaa aagtaaccat tcattcacct
     1621 aaagacgcca ttgccgccgg cattggcatg gttacccaac atttcactct cgtgcccacc
     1681 ctgactgttg ctgaaaacat tgtcctgggt tattcaaaca agatggttct ttctcaaaaa
     1741 gaaatcgaga aggatgttgc ccaggcttct gaaaaatatg gcatcccggt ggacccttcc
     1801 accctggtga agaacctgtc agttggagaa agacagcgcg tggaaattct caaagcgtta
     1861 taccgcaacg cgcgcatcct cattatggat gaaccgacgg ctgtgctggt gccacaggaa
     1921 gttgatgttc tgttcgaatc cctcgtcaga ttggtgaaat ctggcctttc cgtgatattt
     1981 atcagtcata aactcgtcga agtgatgact gtttgcgaca gaatcaccgt gctgcgcgac
     2041 ggcaaggtcg ccaatacggt taaaaaatcc gaaaccaccc aggttgacct ggcaaaaatg
     2101 atggttggtc gtgaaacttt cggcgtccag cgccagtctg cacgtgaagc gagcgaacct
     2161 gtctatgaag tcactgaaat caccctcgaa ggaaaacacg ggcgcaaagt gctggataaa
     2221 gtcactttta acgtcggtgt aggcgagatt ctcggtatcg ccggagtatc aggtaacgga
     2281 cagggagagc ttactgaggt gcttaacggt cttgtaaaac ccagcagcgg cacaataaaa
     2341 atcaatggga aagacatcac ctgctgctca ccgagcgcca tttctgattg tggcatcggt
     2401 cgcacgccgc aagacagaca cgaaggcacc atcggtgaac tcaccgtggc agaaaacctt
     2461 gctctggagc atatttccaa ttaccatcgt aaagggatgt tggatcggaa aaaaatccag
     2521 gaagacgctg cgcagaccat tcaggaattc cagatcaaag cctctccagg cgaccagttg
     2581 cgcaagttat ccggcgggaa tatgcaaaaa gttgtgctgg cgcgcgcttt atcgcgtaaa
     2641 ccaaaagtgc tcattgcttc acaacccacc cgcgggttgg acgtcggcgc gaccgaatat
     2701 gtacgcaaca ggctgctcaa agaaagagaa cgcggtactg cggtggtctt gctctcagaa
     2761 gacctggacg aagtactggc gttgtctgac cgcatcgcag tgatctatga aggcagtatc
     2821 cggggtatca tcccggcggt tgaagccacc cctgagaagc tggggctgct gatggccggc
     2881 gccgcttagg cttatttctc gcattggagg atttattgtg atcaaattcc gacttgaacg
     2941 atctcccgct ccggtttgga ccaaagcgct catcccggtg gtagcggtgc ttgtaacatt
     3001 cgctattaca tccattttta tactcctggc gggggcgaat ccatttgccg catattacaa
     3061 ctttatcatc gtccctcttt ccaccaagtt ctctgccctt gaagtgctcg taaaagccac
     3121 tcctctcatc tttacaggta tcgcggttac ttttgctttc gccgccggtt actataacat
     3181 cggcgcggaa ggtcaacttt acgctggcgc ggttgcggct gcctgggtag gcgccacatt
     3241 caccaccctc ccctctattg tggtcatccc catgatgctg gtgctgggtt ttctcagtgg
     3301 catgctttgg gcgctcatcc cggcattgct gaaagccagg ttgaaagtgg atgaggtggt
     3361 gacaaccctg ctgatgaatt ccatcatcat gtatttcatc tccgcgctgc ttaacggtcc
     3421 ctggcgcgat cctgaaacgg gatggccaaa atcaccagag ttcttcgagg ctgcaaggtt
     3481 tatgcagctt attcccaagt cacgcctgca catcggtttt atcatcgctc tggtggtggt
     3541 ggtggtcgtt tacatcatca tcaagcgcac accatttggg ctgcaaatgc gcgctgttgg
     3601 cgccagtaag gctggagcgc agttcgctgg tatcaacgta aaccgcacca tgctcatcgc
     3661 tgctttggtc agcggcggta tcgccgggtt ggctggcgtg agcgaaattg ccggtattca
     3721 ttaccacctc atcagcgaac tatcaggcgg ctatggctac accggtatca tcgtagccac
     3781 cctcggcgga ttgaacccaa ttggcgctac catcgcggcg ctgtttatcg ggttgatcaa
     3841 caccggcgcg caaacggtga gccgcgtgtt aggcgtacct atttacctgg gaaatgtggt
     3901 ccaatctacg cttttgcttg tcaccctggg catgctgctg cttcagaatt atcggattaa
     3961 gaggatatga tcatgggaga ctttctggtc gatcttatcg cagccacgct gcgtatcgca
     4021 acaccgctta tcttcgccac gctcggagag cttttctgcg aacgtgcagg aattttaaac
     4081 cttggcgttg aaggaaccat gttttttggc gcgtttagtg ggttcaccgc cgcgtcgttg
     4141 accggttctt tatggatggg cattctttgc gcctttattg gtggaatgat ctccggtctg
     4201 atcatgagcc tgttctccgt aagattgggg gtgaaccagc acgtctctgg tttaggggtg
     4261 accctcttaa tgactgcgct ctcgttattt gggttccggg tcattttcgg cgaaaaccgt
     4321 attctgccgt cgattcagcc tttccaacaa ctcagtatct tctccggaag ccgtgtgctg
     4381 ggtccaattt tctcgcagta taccctcacc tacatcgccc tggctcttat tcccatcgtt
     4441 tggtgggtgg tcttccgtac gaattttggg ctgaacctgc gttcagtggg agataaccca
     4501 gaagcgactg acgcggctgg cattaatgtg tatcgcctgc gcaccatcgc cctggtcatc
     4561 ggcggcgcgc ttatgagcgt gggtggtgct tacctttcag tggcacaact tggctcgttc
     4621 accttcggta ttgtcgctgg acgcggctgg gtatgcatcg ccctcatcat cttcgctaac
     4681 tggcacccgg taagggtgtt gtggggcgcg cttctgtttg ggggagtgca ggcgctgcaa
     4741 tccaggctgc agttgaccgg tctcaagctc ccttacgagc tctttctcgc cctgccatac
     4801 atcgtcacca tcatcgcgct caccatcgcc ggtcgcaacg catcttaccc cgcggcatta
     4861 ctcaaaccct ataaaagaga gtgagaattt tttctactca gttcaaaaag catgtgattc
     4921 aactcggtca catgcttttt ttattctttg ctgtaagatt tatgtgacaa tcaaccgttc
     4981 aggatgctaa atgtatcact tgtaatgcca gatggataag cataaactat aattgttctt
     5041 aaaacatcaa aattcaaaga tggaggtagt aatggcgcaa gattcaacag acaagatagc
     5101 caaattgaag gcccttcgcg aagagagccg gctaggcggc ggcgaaaaaa ggatcgcagc
     5161 tcagcacgaa aaaggcaaga agacagcccg tgagcgtatc gacgccctct tagacaaagg
     5221 atccttcaac gaaatcaatg gcttgatgaa aaatcgccat accgatttcg gtttagacaa
     5281 agacaagatc cctggtgacg gtcttgtcgc tggctttggc accatcaacg gcagaaaagt
     5341 ctgcgtttac gcgcaagatt tcaccgtaat gggcggctct tttggcgaag tcgctggtga
     5401 gaaggttgcc cgtgtgatgg acctggcgat ggacgccggc gtgcccgtga ttggcatcaa
     5461 tgacggcggc ggcgcgcgca ttcaagaagg catctacagc ctgtatgcct atggcgaggt
     5521 attctaccgc aacgttcagg cttcgggtgt ggtgccgcag atcagcgtta ttatgggacc
     5581 ctgcgccggc ggcggcgttt actctcccgc cttgcaggac tttatcatca tgacccaggg
     5641 catggggaac atgtacatca ccggaccgga agtgatcaaa gcggttacca atgaaatcgt
     5701 ggaccacgaa acattgggcg gagccgtcac ccacaccacc gtttcgggtg tcgcgcactt
     5761 cctgggacag gatgaggatg acaccttcca tatcatccgc aaaatcctcg agtacatccc
     5821 cgataacaac gccagcactc cccctgttct cccaaccaat gatagcccat tccgcgccga
     5881 caaagaattg aacaccctgg tacccgctga ccccaaccaa acttacgaca tcagggacgt
     5941 gatcaatcgc gtagtggata tcgattcgtt ctttgaagtt caccataact tcgccaccaa
     6001 cgcggtggtt ggttttgccc gcttgaatgg tgaaagcgtg ggaatcgctg caaatcaacc
     6061 tgcttctatg gcgggcgttc tggatatcaa ctcctctgac aagattgccc gtttcgtgcg
     6121 tttctgcgac gccttcaata tccccgtgat cacctttgtg gataccccgg ggttcatgcc
     6181 cggtacacac acagagcaca acgggattat tcgccacggc gcaaaaatcc tgttcgcctt
     6241 ctcagaggct tcagtaccca agatctctgt tgtcacccgc aaagcatacg gcggcgccta
     6301 catcgttatg ggcagcaagc acctgcgcgc tgacatgagc tttgcctggc ctggcgcgga
     6361 aatcgcggtg atgggcgcgg gcggtgcagt gaatatcctt cacggaagat cgctcaagga
     6421 taaagatccc gaagaagcca agaaattgcg cgctgaattg gtagcagatt acgaggataa
     6481 attctccaat ccttaccagg ctgcttctgc tggttatatt gacgaggtga tcatccccag
     6541 tgaaacccgc cagcgtctta tcgccactct cgatatctta aggaacaagc agtccacttc
     6601 tcctgccaag aaacacggga acatcccgct gtaacagaaa ggatatggat aatggaaaac
     6661 ctcggactag cgttaaaaat atccggaatc ggcatcgtgg tgctcctact tgtcctcgct
     6721 atcctggcag ggatcatcta cctgcttacc ttggttctgg ttgacaagga agatgaggaa
     6781 gaaagcaaag acaccgtagt agacgcaaca actgaaccgg agaaagaaaa agcggattcg
     6841 aaatcgcatg ccttactggc agccgccctg gcagtggcga ttgcgcgggc aaaagctgaa
     6901 tcggttgttt cattcgcaga tgcttccacc ggagaagtca atccctggcg ccagtttggg
     6961 cttcagcgcc ggctaacaca atcttccaca atcaggagaa caagatgaca caatacaaga
     7021 ttaccgtcaa ggacaccacc tacgatgttg agatccttga tgacccacgc aatgatgaag
     7081 ttttagtaaa agtcaatgga gaggaactga cagttgtcac tgaagaagtc aacaaagcgg
     7141 tttcagctgt caaagccgcc aaacccgcgc ccgcccgtgc ggccgcgcct gcgcctaaac
     7201 ccgcagcggc tgctcctgtg gctgccggtc ctggcacaat caaatcacct cttcccggtg
     7261 tggtcaactc gattaaagtc cgcgcaggcg ataaggttaa agccaatgac gagatttgtg
     7321 tcattgaagc catgaaagcc atgaatatca tccgcgctcc ccaggatggt acagtcgctc
     7381 gcatccatgt gaatgagggc aaccaggttg cttatggtgc tccgctcatg gatatcgagt
     7441 aggcggaaag gacacgatca tgggcttcga tttatcaggt ttgctcggag gacttctcga
     7501 tatcacatgg cagcagctca tcatgattgc tgtggctcta ctcctgttgt acctcgggat
     7561 ttttaaggat tatgagccca cgctattgat accgattggt ttcgggtgta tcctggggaa
     7621 cctgcccgtt gctgccaaca tgatcggtga ggaaggctta tttggagtcc tctaccgagc
     7681 agggatcagt aacgaactct ttcccctgtt gattttcctg ggtgttgggg cgatgctcga
     7741 cctgcgcccg ctgctcagcc agcctgttat gttcgtgatg ggcgcgctgg ctcacctggg
     7801 aatttttgcc acgttggtca ttgccatcct gctgggcttc aacctcgctg aagccaccgc
     7861 cattggcatg atcggcgcca tcgatggtcc aaccgtcatt tacgtcaaca gtaagttagg
     7921 ctcgctgttt gtaacccccg gtcttgcagc cgcaattgcg gtcacggcgt attcctatat
     7981 gagccttgtg cctatcatcc agcctcctct gatgcgcctc ttcaccacca aaaaagaacg
     8041 cgcaatccgc atggaataca ccccaagacc ggtttccaga atgacggtca tccttttccc
     8101 catcctcatt actgcggtcg ccggcatctt cctgccagaa gccgcgccac tcatcgccac
     8161 cctgatgctt ggcaacctca tcaaagaatc agaagttgtg ccagggttgg tggagacctc
     8221 taagaacgcc atcaccaata cagccacaat cttcctgggt atcaccatcg gctcaaccat
     8281 gcaggcagcc actttcttga acacgggcac cctgcgggtt cttatcttgg gcttgattgc
     8341 tttcgccctc aacaccatcg gtggtctact cttcggaaaa ctgatttgcc tcctttccgg
     8401 gcgcaagatc aacccgctcg ttggcgcagc cgccatcagc gccttcccga tgagcggtcg
     8461 cctggcgcag aaagtcgcgc ttgaggttga taatcagaac ttcatcctca tgcacgcgct
     8521 tggcgccaac gcagccgggc aggttgcttc tgttctggca gccggcatcc tcatgcagat
     8581 gatcttcccg atcttgctgc actagtgaga atatgcactt caacaggtat ctcacccact
     8641 tgtaagaaga acagtcatgg agaatgatcc ttaattcccc atgactgttt tcaatttcaa
     8701 caccataagt gttgaaaaaa attcaaaaaa ggagcgtatc atgaaactac ttgatttgac
     8761 catccccatc ggaattggta ctccaccctg gccgacttat gaacccttac aggtgaagta
     8821 cttcaaacgc ctggctccga atggcgccaa cgggcaggtg ctcacccact ccaaccacct
     8881 cggcacccac cttgacggag agatccattt ctacacaccc ggccgtgaca tcgcccagct
     8941 gcccatggat ttcttggtgc acgaagcagc cattgtagac ctgagcgacg tctgcggtga
     9001 ttatgatgtt tacaccagca aaatgatcac cgaccgtgtg gaagtgaaag aaggcgatat
     9061 cctcgtcatc cacaccggtt accatcattt cggctgggat caacccaccg ctgatgaagt
     9121 gcgctatatg accaaacacc cgggtcctga ccacgaattc gtcgattggg cgatcaagat
     9181 gaaattacgc tggattgctg tggactgcgg gtctgctgat cacccgatga acaccatcat
     9241 ccgcacctgg atgccgcgtc aggcgaaaca ggctgaatac gtgttcaaga aaaaattcgg
     9301 caagaccctt gaagagttct ttaccgatga taagtaccag atgatgcaca tcgacctgtt
     9361 ccccaaagaa atcattcacg cggaatgttt tggcggcatg atcgacctgc tgctcaaccg
     9421 ccgtgtggag gttggtttct tcccctggcg ctttgtcgat ggcgaggctt ccattggacg
     9481 cgcggtggca ttcgtcgatg acaaagaata cgatgaactg atgaagaaag ctgccaccat
     9541 gcccaagacc aaattcggtg acgtctatga tcccaagcat gttgaaagcc tgaacagact
     9601 cagcaaagcc aacctcgcct agcatttgcc ggttaggaga ccagaatgaa attcgaactt
     9661 cccgaacacg cgagccagga ggtattagag atcgatgttc gcgagatcga agccttgctg
     9721 cagggcccgg ctatggacct gcccaagtgg ccaggcgccc aggtcaaatt gaaagacggc
     9781 cgcaccctct atatccgcga agcccgccgt gaagaagccc ccaggatgat ggaatacatg
     9841 aagcgcctga tggacgtgga ccatgatttc tacgacatcg tcggcgcccg tgtctattca
     9901 gagatccttg gctggtatcg tcatcgcctt aaagacccct atactctggt tggtctcatt
     9961 gatggggttt gggctggctt tgccaatggt cgggtgatga atgaaaaggt gaacgtcagc
    10021 ctgcacacca tggctttgct gcgcggcgga cgcatcggcg cggtgatgta ctacgccaag
    10081 gcgtattacg cgttcgaaat gattggtaac accgagtggt gggcgactta cgaatcctac
    10141 aacggatggc tgcgttgggg tctcggtatg gcacaacctt cttatccctg gccggaagtc
    10201 cagcatgagc tgggtggcgc gcgtgtttac tacatcacca aaaagtactg ggatcagctg
    10261 gtcaaggcat atgttcagca gatggctggc gcagacctgg atttcaatgt gcctgacgct
    10321 gttcgacagg caaacgagga aatgatcgtt cctgaagaga tcctggtctg acagacttgc
    10381 attacgcgaa aaaacaggag ccgaatctgg ctcctgtttt tattctcaga ctaaacgagc
    10441 agcgaatcca gcccgtacga gataccaatg agggtatcaa cccctgaaga gtgaccgaaa
    10501 tccaccagat gtcgtgcttt ttcaatccca aaggccacct cgggggtgaa aaggtggtct
    10561 atcaacgtta aaaatatttc ttccgcccaa ccacgccctg cgtattccaa ccggttggct
    10621 gagatcgtgg ttgttttctt aaatgccagc cgggtaagtt cttgccacca ttcattcaat
    10681 aatcctggct cagagttgac ggattgcagg tagcggtaat ggtacagcag aaagccggtg
    10741 aggaaatcat cgcctgatgg ggttaacccg ccgccagcgc caaggatttt ttcagccgaa
    10801 gcgaaaaaac cctctttatt ttgacttcga taagccagca ctatctggcg agccgcgtga
    10861 tgaatttcgc gctgaaagac ctccgtctgg tcgtcaggct gccctgccag aaagagaaac
    10921 cccttgtcag gatcgatgct tttgatcgtg tcgagcaata aattggcacg tagtcgctgt
    10981 tcctgcagag aagccaccat cctcgcgggt tgaggtggat gccacacagc cgccccgtgg
    11041 gtgagcagcg taatctcccg cccactgaac acgatctcat tcttgttcgt ttcaaacgtg
    11101 tcacctggct gcagccgggt aaaaagttgc ccaatcgagt taagagtgag gttaaacggg
    11161 ctgcggtaat ctgaattagt caggaaaagc accctgtcat cagtgcgcag aaaagccccc
    11221 ttggaggtca ccccggctac ccacctgcga gccgctgcct tcatcaggga ttcctgcgct
    11281 gcggctccta tcaagcgtac cttgatcgtt tccactcctt aacaccctcc gttcttgaac
    11341 caatccatca aaccacggta taaaattaca ctcccttgag ggagtgtatg gtgccgagag
    11401 ccaggatcgg actggcgacc ccacaatttt cagtcgtgtg ctctaccaac tgagctatct
    11461 cggcaggtaa ggccaattct acccgacgct ccaaggattg tcaagaaatt cgtgttagtt
    11521 cacctaaaat gttactaaga taacatcgtt aggccaaaag aaa
//
LOCUS       DAOSPM010000056        11249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_5756_length_11249_cov_17.499821, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000056 DAOSPM010000000
VERSION     DAOSPM010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11249
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5756_length_11249_cov_17.499821"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PLW25_06135"
     CDS             complement(<1..1079)
                     /locus_tag="PLW25_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOS54102.1"
                     /translation="MSITEDHAQLEILYNVSRQLAASLDLHEVLARVLLLSTSNLGAE
                     RASLIVLDNAHKPCDAVILYKGELSSASHETINAIVRYGLAGWVIHNRQPVILENTRA
                     DARWLERPYEKETAGSPKSALCLPVHAREKIVGVLTIVHPLPGNFNEAHLKLQQAIAD
                     LAGVAIHNARLYEDAKHSRNLYRGLFEGSPDPIFITTLDGQIIDCNRQGIFVSGFDRR
                     FLLRSSIIHLDPLASDVLTRAGAGRKRSSAGDYESSLSIKGQQRLPVEVSAVRVPYRD
                     LDCLQWIYRDTSARHELDALRDNLSAMIYHDLRSPLANIISGLELLAETIPPEAGPDA
                     QQLLQIAGRSSAHMQRLVSSLLDINR"
     gene            complement(1066..2253)
                     /locus_tag="PLW25_06140"
     CDS             complement(1066..2253)
                     /locus_tag="PLW25_06140"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOS54103.1"
                     /translation="MNPSPSQRFTLSCPECGRAFDPLLPQTYCHDCNSPLLSHYPLER
                     LKAELRREEISARPRGMWRWHELLPVEDPHNRVSLGEGDTPLLPLPTIAAALGASNLL
                     LKDESLNPTGTFKSRGMAAAVSKALELGIREFVIPTAGNAGGALGLYAGRAGCAAHVF
                     MPKDTPAPIQYEIRMSGADVHLVDGLINDAARLAAAESRAHGWFDVSTFKEPYRVEGK
                     KIMGYELAEALGWRLPDVIIYPTGGGTGLVGMWKAFDEMQSLGWIDSARPRMVSVQAA
                     GCAPVVRAFEEGAQRIQPWQNAATIAAGLRVPAVFADRLVLRCLYQSGGCAVSVSDEE
                     LTLAQHELARAEGILAAPEGAATLAALKILLKKGWCEYGESIVLFNTGTGLKYFQQDH
                     VDH"
     gene            complement(2350..3054)
                     /locus_tag="PLW25_06145"
     CDS             complement(2350..3054)
                     /locus_tag="PLW25_06145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOS54104.1"
                     /translation="MKRFFFLLGSGVCIAVIVAVYFLWVNTSLFNPRSRDLMKWLRDP
                     AAHPDWRIEAGQRCDGALFSLPTSGYIGYLWNDSFRPGHHHQGIDIFGGGEPGSVPVY
                     AAYDGYLTRLADWKSTVIIRIPADPLQPQRQIWTYYTHMAYPSGESLIAAQFPPGSSE
                     VFVKAGTLLGYQGNYSGTRDKPTGVHLHFSIVKDDGNGRFLNELEIDNTLDPSPYLGM
                     ALNNKAGATTPAVCTP"
     gene            complement(3059..4372)
                     /gene="miaB"
                     /locus_tag="PLW25_06150"
     CDS             complement(3059..4372)
                     /gene="miaB"
                     /locus_tag="PLW25_06150"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOS54105.1"
                     /translation="MKYHVWTEGCQMNVADSERVSSALEHLGYTHTSNAEDADVIVLN
                     TCVVRQSAEDKAYGRLTSLLPIKRDRPEVVINLMGCLVGIKDHQPLRQRFPYVDVFSP
                     PSDPAPLVEYLIQRDARSAEEAETARRFALMDGDLVLPRSSGGQVAGWIPVVYGCSHA
                     CAYCIIPYRRGVEVSRPPEEIYREARSLAAQGVKEITLLGQIVDRYGKDQPAYPTLAG
                     LLRELSGIEGIERLRFLTSHPNWMTDELLECVAELPKVMPHIEVPNQSGDDDVLQAMK
                     RGYTAEDYRRLVQKIRDRIPGVSIATDIIVGFPGETEAQFQNTYDLLAELKLDVVHLA
                     RYSPRAGTLSARSLEDNVPETEKWRRFRALERLQEAIVREIHATYLHTTVEVLFEDKV
                     KKRWRGRTPTNKLVFVETETNLRGMVLPVKIIRTGPWSMLGELVS"
     gene            4517..5926
                     /locus_tag="PLW25_06155"
     CDS             4517..5926
                     /locus_tag="PLW25_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54106.1"
                     /translation="MNKKIGVTLLVLIISSCVLLSAGVIIGAYLLLKAQKNYTDPLLT
                     LRNTPAVATVTAAQQTQAAAPSDGLPPKIAKQMDEIQQQVIAIRGLHMTTDLKRDLLT
                     PEELQDKVINDFFADYSDEDALKDSKVLSTLGLLDEGFDLKQFYLDLYSEQIAGYYDS
                     ETKDMYVIAGEGFGGMERMTYAHEFNHVLQDQNYDLENGMKLNDDYCKVDTEYCAAVS
                     ALIEGDSVSTENEWFLKHSTRQDQKDLFSFQEEYTSPVYDSAPAYMKEDFLFPYTFGL
                     DFVQQLINQGGWAAVDAAYANPPVSTEQILHPEKYPDDRPVPVEMPDLAGMLGDDWSE
                     LDRNVMGEWYTYLVFAKGRSSQFSMDDEKSKTAAAGWGGDTYVYYATADLKEHLFAWR
                     STWDTSSDAGEYFDMSRDYGLARWGIPVQNSNGVISWNSTSDGIITMRRSGPDVLWVI
                     GSSKEVVDDALTEIKDFGK"
     gene            5929..6618
                     /locus_tag="PLW25_06160"
     CDS             5929..6618
                     /locus_tag="PLW25_06160"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOS54107.1"
                     /translation="MPLDLQRIKGLCFDVDGTICDTDDAWVEKITDFFQPVAKIVKCQ
                     KPRQFARWLVMAAESPMNLVMYWLDTLSLDDNFARLFTRMVKRREYKNTAFRLMRGAN
                     ELLTMAEKKYPLAVVSARDEKTTMMFLEQFSLSKHFVEVVTSQTCERTKPYPMPVQHA
                     ANAMGLQARDCVMIGDTTVDILAGRRAGAQTVGLLCGFGTEKELKKAGADVVLNDLEE
                     LQALLFPVGGN"
     gene            complement(6615..>8132)
                     /locus_tag="PLW25_06165"
     CDS             complement(6615..>8132)
                     /locus_tag="PLW25_06165"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOS54108.1"
                     /translation="HGAVNMRSALANDYLTPLAKIVNETGAANTWRSAAYIGLDSWEN
                     PPDNASPLYGGSPVSLLEVGSAYSTLANAGVRSGRLDKATNRIKPVTVLRVNTSRHQT
                     IWQAPLAENAVIISESVSYLVNHVLSDESARWPSLGHPNELEIGMEAAAKMGRADNGH
                     QVWTVGYTPQRLVLVWMGSRSTEKDTPGLDKRMAAGIWHAVIKYASRGLEHKGWQRPA
                     GITQLQVCSPSGMLPTMDCPNLVYDYFLYGNEPTLPDTLFTRFKVNRETGQLATVFTP
                     PELIDEKVFMNVPAEARLWAQSAGIELIPQGYDAIQTIRQDELVNITQPLMFSAVAGK
                     VVIRGTAAAAHFASYSIQVGEGINPESWLRVKESTRSLSEDGVLAEWDTSGLNGLYAI
                     RLTVIDADNAVRSAVTQVTVDNTPPSARITYPLPDQHITPVRGEVTITAQVEDLVGVA
                     RVEWWVDGKRVSEQTAAPYLYQLSESAGKHSVYLKAWDVAGNSIQSEKISFSVAP"
     assembly_gap    8133..8232
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8233..9350)
                     /locus_tag="PLW25_06170"
     CDS             complement(<8233..9350)
                     /locus_tag="PLW25_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOS54109.1"
                     /translation="MRKTSLYLLLKHRSHSRQRHTARASRAFTWLARALAGVVSLVIV
                     AALLIAGYFYALVARDLPSIEILPSLMDRENGELLQPTRLYDRSGVVTLFAYSNEGIE
                     RKFLPVNPDNPEFISPQLVRSAITAIDPTFWSNPGYLLKEWHTVEPLTIAERLVSELI
                     LWDEPPSITRAVRMRILAGQVVSTYGRTRALEWYLNSAWFGRNAYGVESASRLYLGKS
                     AQQVTLAESALLISLLKSPALTPFDAPEAAFNSQKELLRSMRAVNVISPEEYEQALNE
                     VIHLHATNKPDEAAENGFVKIVEKELESILPAQRLRRGGLEVITTLQADLQEQLACAA
                     NTQLNRIAYSDVSGVAPDQPDCDAALLLPTQRFTNTGSG"
     gene            complement(9357..>11249)
                     /locus_tag="PLW25_06175"
     CDS             complement(9357..>11249)
                     /locus_tag="PLW25_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS54110.1"
                     /translation="TLALQLAICYTHAMKSEEQLFKMAKDAIEQGENAAAGEHLVRLL
                     QFDRSNPQYWLWMSAVVETLKEREFCLREVLKLDRKNPTAIHGLRILGYAIEPPEMPP
                     GFDPLKRDWKTSLEIEKEVEQKTLKRKPRTSSWVMLGALVFSISALVFFLVESNRYRP
                     DTSPILKFSLTPPYSATLTSTPTPFTTGAPPLWTLLEATYTPTPIYAATPHKLSEAYM
                     AAMRAYTKGDWVNALVFFQQVVDAEPDSADIWYHIGEVYRFQGMLKAADTAYDNSLKS
                     NVAFAPAYLGKARVSMSSASPDHKHAAQYLQKALELDPGLSEALSALAELQLSEGDAV
                     AALNSLSQYEERFSLSAQVELLRARAYLISGNAKEALSAAQHANRLDITLLEAYKLIA
                     AAMQNNEQVAGSIEPLETYLTYETNDAEALSMMSLALVEAGNLQEAFLYAERALDIDQ
                     RSVPALIARGRVLFEQGAYLEAAETLDSAIEIQETSFEANILKSQVQLTRKLNDSAIE
                     FSRRAYELAKNDRQKATALYWRARAYAAKKQNDSARADLKALLELPVENLPADLREEA
                     RKLLHQLSAATSTPTTSQTRTTTRTPSASATPAPTQSATFSVTRTPTPSPAGSSAQTS
                     SPTPRR"
BASE COUNT         2437 a         2971 c         3268 g         2473 t
ORIGIN      
        1 cggttaatat ccagcagcga actgaccagg cgctgcatat gcgcgctgga gcgcccggca
       61 atctgcagca attgttgcgc gtctggaccg gcttcagggg ggatggtttc tgccagcaat
      121 tccagccctg aaatgatgtt ggcgagcggg gaacgtaaat cgtgatagat catcgcgctc
      181 aaattgtcac gcagggcgtc aagctcatgc cgcgcactgg tgtcgcggta tatccactgc
      241 agacagtcaa ggtcgcggta aggcacacgc acggcgctaa cctctaccgg taaacgctgc
      301 tggcctttga ttgacaggct tgattcataa tccccagcgc tggaacgctt gcggcctgcc
      361 ccagcgcggg tcaacacgtc acttgcaaga gggtccaggt ggatgatcga gcttcttaat
      421 aaaaagcgcc ggtcaaagcc gctaacgaaa atgccctggc ggttacagtc aatgatctga
      481 ccgtccaggg tggtgataaa aattggatcc gggctgcctt cgaacaaacc acgatataaa
      541 ttgcgactgt gtttggcgtc ttcgtataac cgcgcgttgt gaatggcgac gcccgccaaa
      601 tcggcgatgg cctgttgcag cttcagatgg gcttcattaa agttccccgg caaagggtgg
      661 acgatggtga gcacacccac aattttctca cgggcgtgca ccggcaggca taaagcggat
      721 tttggcgaac ctgcggtttc tttttcatag ggtctttcga gccagcgcgc gtcagcacgg
      781 gtgttttcaa ggatcacggg ctggcggttg tgaatgaccc aaccagccaa accatagcgc
      841 acgatcgcgt tgatggtttc gtgtgaagcg gatgaaagct cgcctttata aaggatgacc
      901 gcgtcacacg gtttgtgggc attgtcaagc acgatcaggc tggctctttc cgcgccgagg
      961 ttgctggtgg aaaggagcag cacgcgcgcc agcacttcgt gcaggtcaag actggctgcc
     1021 agttgacggc tgacgttgta aagtatttcc agttgtgcgt gatcttcagt gatcgacatg
     1081 gtcttgctgg aagtatttaa gtccggttcc tgtgttaaat aatacgatgc tttccccata
     1141 ttcgcaccag ccttttttaa gtaaaatttt taaagccgcc agcgttgccg cgccttcagg
     1201 tgcagccagg atgccctcag cacgcgccag ctcgtgctgg gcgagggtga gctcttcatc
     1261 gctcacgctg acagcgcagc ctccgctctg gtaaaggcag cgcagcacca ggcggtcggc
     1321 gaagaccgca ggtacgcgca acccagccgc gatagtagca gcgttctgcc agggttggat
     1381 gcgctgcgcg ccttcttcaa acgcccgaac caccggagcg caacccgctg cctgcaccga
     1441 gaccattcgc ggtctggcgc tgtcaatcca gccgagagat tgcatttcat cgaatgcttt
     1501 ccacatccca accaggccgg taccgccgcc agtgggatag ataatgacgt ctggcaacct
     1561 ccagcccagc gcttcggcga gctcgtagcc catgatcttc ttgccttcaa cgcggtacgg
     1621 ctctttgaaa gtggaaacgt caaaccagcc gtgagcgcgt gactcggcgg cagccaggcg
     1681 cgcggcatca ttgatgagcc cgtccaccag gtgcacatcc gcgcctgaca tgcggatttc
     1741 gtactggatg ggtgccgggg tgtcttttgg catgaacaca tgcgcggcgc agccggcgcg
     1801 cccggcataa agccccagcg cccctcccgc gttgccggca gttgggataa cgaattcacg
     1861 gatacccagc tccagcgctt tcgaaaccgc cgccgccatg ccgcgcgact tgaaggtacc
     1921 ggtggggttg agcgactcgt ccttcagcag caggttggag gcacccaaag ccgcggcaat
     1981 ggtgggcaga ggcaataaag gcgtatcgcc ttcgccaagt gaaacgcggt tgtgcgggtc
     2041 ttccactggc agcaactcgt gccagcgcca catgccgcgc ggtcgggcgc tgatctcctc
     2101 acggcgcagt tccgctttca gccgctcgag cgggtaatgc gaaagcaagg gcgaattgca
     2161 atcgtggcag taagtttgcg gcaagagggg gtcaaacgcg cgtccgcatt ccgggcaaga
     2221 caaagtgaag cgttgtgagg gagatggatt cataaaatga gtatagcata aggggcgttt
     2281 cggatgaaag ggtagcacca aaacgcttca gaaggctctc tgaatgccgg gaggtaaatc
     2341 cttttttgct cagggggtgc acaccgccgg ggtggtcgca ccggctttat tgttcaatgc
     2401 cattcccaga tagggcgaag ggtcaagagt gttgtcaatt tccagttcat tcagaaagcg
     2461 gccgttaccg tcgtctttca cgattgaaaa atgcaggtgc acgccagtgg gcttgtcgcg
     2521 tgtgcccgag tagttcccct ggtagcccag cagggttccg gccttcacaa agacttcgct
     2581 gctgccgggc ggaaattgcg cggcaatcag gctttcacct gatgggtaag ccatatgagt
     2641 gtaatacgtc cagatctgcc gctgtggttg cagcgggtca gcggggatgc ggatgatcac
     2701 agtcgatttc cagtcggcta accgggtgag gtaaccatcg taagcggcgt aaacaggcac
     2761 gctgccgggc tcaccaccgc cgaaaatatc aatgccctgg tgatggtgcc ccgggcgaaa
     2821 ggagtcattc cataaataac cgatataacc gctggtcggc aggctgaaga gggcgccgtc
     2881 gcagcgttga ccggcttcta tacgccaatc gggatgagcg gcggggtcgc gcagccactt
     2941 catcaggtca cgcgagcgcg ggttaaaaag ggaggtgttg acccataaaa aatacactgc
     3001 cacaatgaca gcgatgcaca cacccgaacc caaaaggaaa aagaagcgtt tcatacgctc
     3061 agctgacgag ttctcccagc atagaccagg gcccggtgcg gatgatcttc accggcagta
     3121 ccatgccgcg caggttcgtt tcagtttcta cgaagaccag cttattggta ggtgtgcgcc
     3181 cacgccagcg tttcttcacc ttgtcttcga agagcacctc tacggtggtg tgaaggtagg
     3241 tcgcgtggat ctcgcgcacg atggcttcct gcagccgctc caacgcccgg aagcggcgcc
     3301 acttttcagt ctcgggcacg ttatcttcca gcgagcgcgc cgataaagtg ccagcgcgcg
     3361 gtgagtaacg ggccaggtga acgacatcca gtttcaattc tgccagcaag tcataggtgt
     3421 tttggaactg tgcttcggtt tcaccgggga aacccacaat gatgtcagtg gcaatggaaa
     3481 ccccgggaat gcggtcgcgt attttctgca ccaggcggcg gtaatcttcc gcggtgtagc
     3541 cgcgcttcat cgcttgcaga acgtcatcat cccctgactg gttgggaact tcaatgtgcg
     3601 gcataacttt gggtagttct gccacgcact ccagcagctc atccgtcatc cagttggggt
     3661 gagaggtaag aaaacgcagc ctttcgatgc cttcaatgcc gctcagctcg cgcagcagcc
     3721 cggcgagcgt ggggtaggcc ggctggtctt ttccgtaacg gtccacgatc tgccccagca
     3781 aggtgatttc cttaacgccc tgagcggcaa gcgagcgggc ttcacggtag atctcttccg
     3841 gcggacggct cacctccaca ccccggcggt acgggatgat acagtaggca caggcgtgcg
     3901 aacagccgta aacaaccggg atccaaccgg ctacctgccc gccgctgcta cgcggcagca
     3961 ccaggtctcc gtccatcaag gcgaagcgcc tggcggtctc ggcttcttct gcactgcggg
     4021 cgtcgcgttg gataaggtat tcgaccaatg gggcagggtc tgatggggga gaaaaaacgt
     4081 cgacataggg gaagcgctgg cgcagtggtt ggtggtcttt tatcccgacc aggcagccca
     4141 tcaagttaat taccacttcc gggcggtcgc gtttgatcgg cagcagagag gtgagcctgc
     4201 cgtacgcctt atcttcagcc gactggcgca ccacgcaggt attgagcaca atcacatccg
     4261 catcttcagc gttggaagtg tgtgtgtagc ccagatgttc cagcgctgaa gaaacacgct
     4321 ccgagtcagc cacgttcatc tggcagcctt cagtccaaac atgatatttc ataggcgcca
     4381 ttataacaaa agaagaggtt cgctcgccgg gtcacctttt tacgaactgt gacgaggttc
     4441 tttagggtaa aatgggtgaa atatctcaca gccctctctt gtggatggga cagatgaaag
     4501 gcgatggcta tcaatcatga ataaaaaaat cggagtgact ttactggtgc tgattatttc
     4561 atcctgcgtg ctcttgagcg ccggagtgat cattggcgcg tatcttttgt taaaagcgca
     4621 gaaaaactat accgaccccc tacttacttt gagaaacacg ccagcagtgg ccacggtcac
     4681 tgcagcgcag caaacacagg cggcagctcc gtctgatgga cttccgccca aaatcgccaa
     4741 acaaatggat gagattcagc agcaggtgat cgccatccgc gggctccaca tgaccaccga
     4801 cttgaagcga gacctgctga ccccggaaga attacaggat aaagtgatca acgatttctt
     4861 cgccgattat agcgatgaag acgcgttgaa agactccaaa gtactctcca cactggggtt
     4921 gctcgatgaa gggtttgacc tgaaacagtt ttacctggac ctgtattcag aacagatcgc
     4981 tggttattac gacagcgaaa ccaaagacat gtacgtcatt gctggagaag gttttggcgg
     5041 tatggagcgc atgacttatg cgcacgagtt caaccacgtg cttcaggatc agaactatga
     5101 cctggagaac ggtatgaaac tgaatgatga ctactgcaaa gtagataccg aatactgcgc
     5161 cgcggtgagc gcgctgattg aaggtgactc ggtttccact gagaatgaat ggtttctgaa
     5221 gcacagcacc cggcaggatc aaaaggacct cttctctttc caggaagaat acaccagccc
     5281 agtctacgac tcagcgccgg cgtacatgaa agaagatttc ttgttcccgt acacctttgg
     5341 gcttgacttt gtgcagcagt tgatcaacca gggtgggtgg gcggccgttg atgcggctta
     5401 tgcaaaccct cctgtttcta ctgaacagat cctgcatcct gaaaaatacc cggatgatcg
     5461 acctgttccg gtggaaatgc ctgaccttgc cggtatgctg ggtgatgact ggagcgaact
     5521 tgaccgcaac gtgatgggag aatggtacac ctacctcgta tttgccaaag ggaggtcctc
     5581 ccagttctcc atggatgatg aaaagagcaa aacagccgca gcaggttggg gtggagacac
     5641 ctatgtttat tacgccacag cagacctcaa ggaacacctc tttgcctggc gcagtacctg
     5701 ggacacctcc agtgatgccg gcgaatattt tgatatgtca cgcgactatg ggttggcgcg
     5761 ttggggcatc ccggtacaga acagcaacgg tgttatctcc tggaactcga cctctgacgg
     5821 cataatcacc atgcgccgca gcggtccgga tgtattgtgg gtgataggct cctcaaaaga
     5881 agtggtcgat gatgcactga cggaaattaa agattttgga aaataagcat gccgcttgac
     5941 cttcagcgca ttaaagggct gtgctttgat gtggatggca cgatctgtga taccgatgat
     6001 gcctgggtcg agaaaataac cgatttcttt caaccggttg cgaaaatcgt taaatgccag
     6061 aaaccgcggc agtttgcacg ctggctggtg atggcggctg aatcaccgat gaacctggtg
     6121 atgtactggc tggataccct gagcctggat gataacttcg cgcgcttgtt tacgcggatg
     6181 gtgaaaaggc gcgaatataa aaacacggca ttcagactga tgaggggagc gaatgaacta
     6241 ctgacaatgg cggagaaaaa atacccgctt gctgttgtca gcgctcgtga tgaaaaaacg
     6301 accatgatgt tcctcgagca attctcgttg agcaaacact ttgtagaagt ggtcacctct
     6361 caaacatgtg aacgcaccaa gccatacccg atgccggtgc agcacgcagc caatgccatg
     6421 gggttgcaag cgcgtgactg cgtgatgatt ggcgatacca ccgtagatat ccttgcgggc
     6481 aggcgcgccg gcgcgcaaac ggtaggcctg ttatgcggtt ttggcacaga aaaagaatta
     6541 aaaaaagctg gagcggatgt tgtgctgaac gaccttgaag aacttcaagc cttattgttc
     6601 ccggttggtg gaaattaagg agcaactgaa aaagagatct tttcgctctg gatggagttc
     6661 ccggcgacat cccaggcttt cagatacaca ctgtgtttcc ccgcagattc gctcaactgg
     6721 taaaggtagg gcgctgctgt ttgttctgac actcgtttgc catccaccca ccattccact
     6781 ctggctactc caaccaggtc ttcaacctgc gcggtgatgg tcacctcacc gcgtacgggc
     6841 gtgatgtgct ggtcaggcag gggataggtg atacgcgcag aagggggggt gttgtctact
     6901 gtcacctggg tgaccgcgga gcgaaccgcg ttgtcggcat caatgaccgt caggcggatg
     6961 gcgtacagcc cgttcaaccc gctggtatcc cattcggcga gcacgccgtc ttcgctcagg
     7021 ctgcgggttg attccttaac tcgcagccag ctttcaggat taatcccttc gccaacctga
     7081 atggaataag aagcgaaatg ggctgccgct gctgttccac ggatgaccac tttccctgct
     7141 accgctgaaa acatcagcgg ttgagtgatg ttgaccagct catcttgcct gatggtttga
     7201 atagcatcat acccctgtgg gattagttca atcccggcgc tttgcgccca gaggcgcgct
     7261 tctgctggga cattcataaa cactttttca tcgatcagtt cgggaggtgt aaacacggtt
     7321 gccagctgac cggtttcgcg gttaaccttg aaccgggtga agagcgtgtc tggcagagtg
     7381 ggttcgttcc cgtaaaggaa atagtcatag acgaggttgg ggcagtccat ggtcggcagc
     7441 atgccagatg ggctgcatac ctgaagctgc gtgatgccag ccgggcgctg ccagccttta
     7501 tgctccagcc cgcgagaagc atacttaatc accgcgtgcc agataccggc tgccatccgc
     7561 ttatctaatc caggggtatc cttttcagtt gaacgggaac ccatccatac cagtaccagg
     7621 cgctgcgggg tatagcctac cgtccaaacc tggtgcccgt tatccgcgcg tcccattttg
     7681 gccgcggctt ccataccaat ttcaagttcg ttggggtgcc ccaaacttgg ccagcgcgcg
     7741 ctttcatcgc tgagtacatg gttgaccaga taagagacgc tttcactgat aatgacggcg
     7801 ttctcagcca atggcgcctg ccagatggtt tgatggcggc tggtattgac ccttaacacc
     7861 gtcaccggtt ttatgcggtt ggtcgcctta tccagccggc cgctgcgcac gcccgcgttt
     7921 gccagcgtgc tgtaagcgga gcccacttcg agcaggctca cagggctgcc gccgtacaac
     7981 ggggaagcgt tatcaggcgg gttttcccac gaatccagac caatgtaggc agcagagcgc
     8041 caggtgttgg ccgcccctgt ttcattgaca attttcgcga ggggggtcag gtaatcgttg
     8101 gccagggctg aacgcatgtt caccgcgcca tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nncccgaccc ggtgttggta aaacgctgag tgggcagcag cagggcggcg
     8281 tcgcaatcag gctggtccgg tgccacgcca ctcacgtcac tgtaagcgat gcggttcaac
     8341 tgggtgttgg cagcacaggc gagctgctct tgaaggtccg cctgcagggt ggtgatcacc
     8401 tccaggccgc cgcgcctgag ccgctgggcg gggaggatgg attccagctc cttctcgacg
     8461 attttcacga aaccgttttc ggctgcttca tcaggcttat ttgttgcgtg caggtgaatt
     8521 acctcgttca gcgcctgttc atactcttcc gggctgatga cgttaacggc gcgcatactg
     8581 cgcaatagct ctttttgtga attgaaggcg gcttcaggcg catcaaacgg ggttaacgcc
     8641 gggcttttca ataacgaaat taacagcgcc gattcagcca gggtgacctg ttgagcggac
     8701 ttacccaagt acaggcgcga cgcactctcc acgccgtatg cgttacgacc gaaccaggcg
     8761 ctgttcaggt accattcaag agccctggtt ctaccatagg tggagacgac ctgcccggcg
     8821 aggatacgca ttcgaacagc gcgggtgata gagggcggtt catcccaaag aatgagctcg
     8881 ctcaccaacc tctctgcgat ggtcaggggc tcaacagtgt gccactcttt caagagatat
     8941 cccgggttgc tccagaaggt tggatctatc gcagtgatgg cgctgcgcac caattgaggg
     9001 ctgatgaatt ctggattatc agggttgacc ggtaaaaact ttctttcaat gccttcattt
     9061 gaataggcaa aaagggtaac cacgccggaa cggtcataca acctggtggg ctggagaagc
     9121 tcgccattct cgcggtccat caagctgggc aggatttcga tcgagggcaa gtcgcgcgcg
     9181 acgagcgcgt aaaaataccc cgctatcagt agtgctgcaa cgatcaccag tgaaaccaca
     9241 cccgccaggg cgcgtgccaa ccaggtaaac gcgcggctcg cccgggccgt gtggcgttgc
     9301 cgtgaatgcg aacggtgttt gaggagcagg taaagcgacg tcttgcgcac gggtttttac
     9361 cttcgcgggg tgggagaact tgtttgcgcg ctgctgcctg ctgggctggg ggttggtgta
     9421 cgggtgacgg agaaagtggc gctctgcgtt ggtgcaggtg tggcgctggc tgagggagtg
     9481 cgcgttgtgg tgcgggtttg actggttgtg ggtgtggatg tagcggcaga taattgatga
     9541 agcagtttcc gggcttcttc acgcaggtct gccggaaggt tttccacggg caattccagc
     9601 aaggctttca agtcagccct ggccgaatcg ttttgttttt tcgccgcgta ggcgcgtgcc
     9661 cgccaataga gcgcggtggc tttttgccga tcattcttgg ccagctcgta ggctcggcgg
     9721 gaaaattcaa tggcgctatc atttaatttc cgggtcaact gcacctgcga tttgaggata
     9781 ttggcttcga atgaagtctc ttgaatctcg atggcgctgt ccagcgtttc agcggcttca
     9841 agataagcgc cttgctcaaa caagacgcgt ccacgggcaa tgagcgccgg cacggagcgt
     9901 tgatcgatgt cgagggcgcg ctctgcatag agaaaagcct cctgcaggtt gcccgcttcg
     9961 acgagcgcca gcgacatcat cgacagggct tcggcgtcat tggtttcgta ggtcaggtag
    10021 gtttccagcg gttcaatcga gcctgcgacc tgctcgttgt tttgcatggc ggcggcgatg
    10081 agcttatacg cctcgagcag cgtgatgtcc aaacggttgg cgtgctgcgc ggcggagagc
    10141 gcttctttag cgttgccgct gatcaggtag gcgcgggcgc gcagcaattc cacctgggcg
    10201 ctgagagaga atcgttcttc atactggctt aagctgttga gcgcggcaac agcatcaccc
    10261 tctgataact gcagttcagc cagcgcggac agggcttcag agagccccgg gtcaagttcg
    10321 agcgcttttt gtaaatattg ggcagcgtgc ttatgatcag gtgatgcgga agacatagaa
    10381 acacgcgctt tccccaggta ggcgggcgcg aaagccacgt tactcttgag gctgttatca
    10441 tatgcggtgt ccgccgcttt taacatccct tgaaagcggt aaacctcgcc gatgtgatac
    10501 cagatatctg ctgaatcggg ttcagcgtcc accacctgct ggaaaaacac cagggcgttg
    10561 acccaatctc cttttgtgta ggcgcgcatg gctgccatat acgcttcaga gagtttgtgc
    10621 ggagtggccg cgtagatagg cgtgggcgtg taggtggcct ccaacagcgt ccataaaggg
    10681 ggagcgccgg ttgtaaacgg ggttggcgtg ctcgttagcg tggcagaata aggcggcgtt
    10741 aaggaaaact tcagaatggg cgaggtatcg ggccggtaac ggttggattc taccaggaag
    10801 aagaccaaag cggagatgct gaaaaccagc gcgcccagca tcacccagga ggaggttcgc
    10861 ggcttgcgtt tcaacgtttt ttgctcgacc tctttttcaa tttccaggct ggttttccaa
    10921 tcgcgcttca gcggatcgaa cccagggggc atctcggggg gctcaatggc atatcccagt
    10981 atacgcagcc cgtgaatggc ggtggggttt ttacggtcaa gcttgagcac ctctcgcagg
    11041 caaaattccc gctctttgag ggtttctacc acggcgctca tccacagcca gtattggggg
    11101 ttagagcggt cgaattgcag caaccgcacc aggtgttcgc ccgcggcagc gttctccccc
    11161 tgttcaatgg catctttcgc cattttgaac aactgctctt ctgatttcat ggcatgagtg
    11221 tagcatatgg ctaattgcag agcaagcgt
//
LOCUS       DAOSPM010000057        11193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_5797_length_11193_cov_13.492907, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000057 DAOSPM010000000
VERSION     DAOSPM010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11193
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5797_length_11193_cov_13.492907"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PLW25_06180"
     CDS             complement(<1..1145)
                     /locus_tag="PLW25_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOS54111.1"
                     /translation="MTIHFGTDGWRAVISDTFTFTNLRLVAQAIADAVATPEWVNGNR
                     IGSEIDAHKMVVGFDTRFLSDRYAAEVARVLAANGFVVYLAQGDAPTPVISHAVRSLN
                     AIGGVMITASHNAPRYNGVKLKSASGGSALPEQCRRVEVYLNDNEALARGPNLMDMEQ
                     ARALKLVQRFNPVPAYYEHLRTLIKSDVIAENPQRFVVDSMYGSGRGIVRGFLQGTGC
                     EIAEIRGEMNPGFGGVHPEPIRQNLGALAGAIGAGMGSFGIATDGDADRIGAMDERSN
                     FVDPHKIMALSLRYLVEKRGLTGSVVRTVSTTRMIDRLAKKYGLTVHETPVGFNHIAD
                     YMLKEQVLIGGEESGGIGFSGHIPEGDGILMGLLIVEMLADAGTTLF"
     gene            complement(1170..2390)
                     /locus_tag="PLW25_06185"
     CDS             complement(1170..2390)
                     /locus_tag="PLW25_06185"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOS54112.1"
                     /translation="MSDYSQEPGVVIPPSRIDESRRRRARKLLVPRGKSERALYLGEI
                     AQRLVPGVEFFLFSLVAGLVITLAILLDSPAIFILGALLAPFMAPVIGLGFSAVIGSF
                     TFFSRSIGAMFIGCALVFASGALGGWISRLFVDLPLSQSLRFTNFSIPDFLLLTIGIV
                     LAVYLTIRMPKQRTQVASVPLAYEIFIPVAVAGYGLTSRASGLFPNALIVAGVHIAWV
                     ILAGTLTLAVLKIRPFTFFGYLLTAAIFGSALYGLVISSALGVALQRQLAPFVTSTGV
                     PSAALIEPSASLSPADALAIQTPDPFGITATTAPTNTLMPTLTPTVTITPQPTPVWAK
                     VFSPTYSGVLVRQTPGFDGKYLTSLDNESLIQVLPEIEHVDGAYWAHVLLEDGREGWI
                     MRSLLLTATPAPNW"
     gene            2592..3632
                     /locus_tag="PLW25_06190"
     CDS             2592..3632
                     /locus_tag="PLW25_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54113.1"
                     /translation="MDRHNRLHLKRILSLLFIIGLLMTGCSKPTDQPPVPSATPLTQE
                     PVDTPTAIPTAAPARMLLLDPFAAATAELNAYLAGFAEQHDLTLETQASPELPALGAE
                     TKVVLLLAELPGLDELTATSPETQFILIGNTALNPAGNLSVIQTREYDLTFMAGYLAQ
                     SIAWDWRTAGLIPSDTALSEPKTNAFINGARYLCGVCTPFYAPIVSFPMLAQEPAQAD
                     AATWSGQVSVFGQHFVNTYFVDPAVATVETLDGLKGLEPAIFNDVKLIGISTTPNPER
                     FTALIGFDPLPALEQLLPQLIAGNGGLSLGAQVKITSFTDDTVITPAKIDNFNRVAAD
                     LATGMIVPLSIP"
     gene            3926..4360
                     /locus_tag="PLW25_06195"
     CDS             3926..4360
                     /locus_tag="PLW25_06195"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOS54114.1"
                     /translation="MNVEIKISKDITEPYAVIYTNAITEEVQKIRSVLAFQSPVIPVS
                     DDEKIVVLEPDEIFMIRTENTEVIVYGKDTRFRSEKRLYELEDLLGNGFLRISKSTIV
                     NLKKIDYVEPSFNGMMAIRLKNGGKDYISRKYLPQLKNYLGI"
     assembly_gap    4411..4510
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4612..5322)
                     /locus_tag="PLW25_06200"
     CDS             complement(4612..5322)
                     /locus_tag="PLW25_06200"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOS54115.1"
                     /translation="MESAPEVKWLYYNVKSSVNARSTMDFGTIKPMTRFLKRLFDLLA
                     SALLLILLSPLLLILALLVRFKLGRPVLFSQERPGLRGHPFKLYKFRSMRDAYDDSGK
                     PLPDEQRLTPFGRFLRSSSLDELPELFNVLKGEMSLVGPRPLLTAYLERYTPDQARRH
                     EVLPGITGWAQVNGRNALSWEEKFELDVWYVDHQSLWLDIKILFITLWKALKREGINA
                     PGSATAPEFMGTGKPSFK"
     gene            5321..5563
                     /locus_tag="PLW25_06205"
     CDS             5321..5563
                     /locus_tag="PLW25_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5351..5353,aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HOS54116.1"
                     /translation="MIISIEYCVVUHYTPRAVGLAAELLEAYETKIKSVTLIPSSGGA
                     FEVNVNGNLIYSKLQTGRHAEVGEVVALLRKKIEEG"
     gene            5566..6570
                     /gene="trpS"
                     /locus_tag="PLW25_06210"
     CDS             5566..6570
                     /gene="trpS"
                     /locus_tag="PLW25_06210"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOS54117.1"
                     /translation="MTSKKGRVFSGARPTGRQHIGNYFGAIRNYVKLQDDYDCIYCIV
                     DLHALTTLDTTRDLRQNTLEMAWDWLAAGMDPKRSIIFVQSMVRQVTELHTILSMVTP
                     LGKLTDLPTFKEKVRENPKNINYGLVGYPVLMSADIALYRADVVPVGIDQAPHVEFAR
                     ETVRSFNFRYNTNALIEPKEKFTEFPKILGIDGVNKMSKSLNNDIELAATAEETTARV
                     MQMVTDPARQRRSDPGNPDICNVFSLHKMFTPPEKVAEINIECRRAGIGCVDCKRILA
                     ANMNAYFAPLREKRRVFENDPEGTWDMLRDGAARASLLAEETMVDVRAAVGLPALQKG
                     "
     gene            6577..>6774
                     /locus_tag="PLW25_06215"
     CDS             6577..>6774
                     /locus_tag="PLW25_06215"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOS54118.1"
                     /translation="MRAVIQRVNYGRVVIEDRETANIGSGMVILLGVGPGDDEQKSAA
                     LAKKIAQLRIMEDAEGKMNLSL"
     assembly_gap    6775..6874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6902..7933
                     /gene="selD"
                     /locus_tag="PLW25_06220"
     CDS             6902..7933
                     /gene="selD"
                     /locus_tag="PLW25_06220"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:6935..6937,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOS54119.1"
                     /translation="MRLTSLSNCAGUASKLSAEALAQVLRPMQELFDPADTPELLAGL
                     ASPDDAAVWKLDEDRALVLTTDFFTPVVDDPFDYGRIAAANSLSDVYAMGAAPFLALN
                     IAAFPPDLDPAVSGEIFRGGATVAKQAGAVIAGGHSIQDKEPKYGMVVAGMVDAQRMF
                     SKGGAKPGDRLYLTKPLGFGTITTALKRQMVREEDLAEAVSWMVRLNREASRIGMDCG
                     VRAATDITGYSFIGHALELANASGVGLRVAFERLPVLPAAFTYASQFIFPGGAYDNRS
                     YFQPFVEFSRKLPEDQTMLLFDPQTSGGLLLAVPAQHCADFEAAMQQGSAPCWQVGEV
                     IKGSGIIFT"
     gene            7984..9846
                     /gene="uvrC"
                     /locus_tag="PLW25_06225"
     CDS             7984..9846
                     /gene="uvrC"
                     /locus_tag="PLW25_06225"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOS54120.1"
                     /translation="MVELTDHLKGILDTLPVKPGCYIMKNASGEVIYVGKAVNLRGRV
                     RSYFQEHADHERKVLKLVSQIADLEWIVVGSELEALILEMNLIKKHRPRFNVRLKDDK
                     RYPYIKVHLASDFPKVTVTRQMVNDGSRYFGPYTSVWAVHQTLDLLRRIFPYLTCDRE
                     ITGLDARPCLYYDIKLCSGPCIGAIDRAGYNQLISDLCAFLDGRTDPIVSRLQREMND
                     AAESLNFEKAALLRDQLQSIERVVEKQKVITSEQIDSDVLAIARADNEACVQVFFVRN
                     GRLMGREYFVMEGTEEIPDEQVMAEFIKQFYTEAAVIPDRVLLPNEVQEAQIIRQWLN
                     TRRGGNKVELRVPRRGTAHELMKMATENAVETLQALRAQWEADKNKQTQALSEIQQAL
                     NLPEPPNRIECYDISNTQGTTSVGSMVVFEQGTPSRAHYRQFNIKTVSGADDFASMEE
                     VLNRRFKRWQSAQEKQGLGAKPDPSFALLPDLLIVDGGKGQLSRAVKVLADYNLAGVV
                     PVVGLAKQQEELFQPGSGSSVVLPRHSQGFYLLQRIRDEAHRFAITAHRKARGRVGVA
                     SRLESIPGIGPARRRALLQQFGDIERIRQASLQELMSVPGINEELARAVQAGLE"
     gene            complement(9867..10313)
                     /locus_tag="PLW25_06230"
     CDS             complement(9867..10313)
                     /locus_tag="PLW25_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOS54121.1"
                     /translation="MATKTSKKKQQKQTKWLLPLILVSVAVIIGVVLSIAKEGHAAKG
                     LPSTVSVTEAAQRINEGAFLLDVRTAEEWNQAHVAGAVRIPLDELKSRLAEVPVDQDV
                     LIICRSGNRSGQARDLLRAAGLKRTTSISGGINAWMAKGLPVVSGP"
     gene            complement(10372..>11193)
                     /gene="pip"
                     /locus_tag="PLW25_06235"
     CDS             complement(10372..>11193)
                     /gene="pip"
                     /locus_tag="PLW25_06235"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HOS54122.1"
                     /translation="HGGPGVGAQPLYRRFFHPERFRVINFSQRGAGKSSSSGELQQND
                     TWALVEDIEKLRQAVNVERWFVFGGSWGSTLALAYAQQYPERVRGLVLRGIFLGTRAE
                     HNWLYVDGASRVHPESWQRFQSFIPPEERSDLPAAYYQRLTSIQREVQMAAAEHWYHW
                     EESLISLLPREAPRMSPDELLAFARIECHYIVNRLFLPDDNYLLNNAHKIAHLPCWIA
                     QGRYDIICPVVSAINLCQHLPEAELHIVPDAGHAISEPGIVDSLMNGIEALAERG"
BASE COUNT         2515 a         3021 c         3120 g         2337 t
ORIGIN      
        1 tcgaagaggg ttgttccagc gtcggcgagc atttccacga ttaataagcc cattaaaata
       61 ccgtcgccct ccgggatgtg accgctgaag cctattccgc ctgactcctc cccaccaatc
      121 aacacctgtt ccttcagcat ataatcggcg atgtggttaa aaccgaccgg ggtttcgtgc
      181 actgtgagcc cgtacttctt ggccaggcgg tcgatcatac gtgtggtgga aaccgtgcgc
      241 accacagaac cggtcaggcc acgtttctca accaggtagc gcagtgacag ggccatgatt
      301 ttgtgcgggt caacgaaatt gctgcgttcg tccatcgcgc cgatgcggtc agcatcgccg
      361 tcggtggcga tgccaaaact acccatccct gccccaatcg cgccagccag cgcgccgagg
      421 ttctggcgga tgggctccgg gtgaacccca ccgaagccgg ggttcatttc tccgcggatc
      481 tcagcgattt cacagccagt gccctgtaga aacccacgca cgataccccg tcctgagccg
      541 tacatagaat caacgacaaa gcgctgcggg ttctcggcaa tcacatcact tttaatgagc
      601 gtgcgtaaat gttcgtaata agccgggacg ggattgaagc gttggaccaa cttaagggcg
      661 cgcgcctgtt ccatatccat caagttcggc ccgcgtgcca gggcttcgtt atcgttcagg
      721 taaacttcta cccggcgaca ttgttccggc agcgctgaac cccccgacgc ggattttagc
      781 ttgaccccgt tataacgcgg ggcgttatga gaggctgtga tcatgacgcc gccgatggcg
      841 tttaaactgc gcacagcgtg cgaaataacc ggggttggcg cgtctccctg cgccaggtac
      901 acgacaaagc cgttggctgc cagtacgcgc gccacttcgg cagcgtagcg gtccgaaagg
      961 aaacgcgtgt cgaacccaac caccatttta tgcgcatcga tttcggagcc tatccgattg
     1021 ccgttgaccc attcaggggt agcaaccgcg tcggcgattg cctgcgccac caggcgtaaa
     1081 ttggtaaagg taaaagtatc cgagattaca gctcgccagc catcagtgcc aaaatgaatc
     1141 gtcattggtt ttctccagtg aaatgtattt taccagttgg gtgctggtgt ggcggtaagt
     1201 aacaggctgc gcataatcca gccttcccgt ccatcttcaa gcagaacgtg cgcccagtaa
     1261 gcgccatcca catgttcaat ttcggggagt acctggatca gcgactcgtt atccaggctg
     1321 gtgaggtatt ttccatcaaa gcctggggtt tgcctgacca acacaccaga atacgtaggg
     1381 ctgaacacct ttgcccacac cggtgtgggt tgaggggtga tggtgactgt cggggtaagc
     1441 gtgggcatca gcgtgttggt aggggctgtt gtggcggtga tgccgaacgg atcgggcgtc
     1501 tggattgcca gcgcgtctgc cggagacagg ctggctgatg gttcaatcaa cgctgcggag
     1561 gggacgcctg tggaggtcac aaatggcgcc agttgacgct gcagggcaac acccaacgcg
     1621 ctggaaatga ccaacccata caacgcactg ccgaaaatgg ctgcggttag caggtacccg
     1681 aagaaggtga acgggcgtat cttgagcact gccagggtca gggtgccggc aaggatcacc
     1741 caggcgatgt gcacgccagc cactatcagg gcgttaggga acaaaccgct tgccctggag
     1801 gttaacccat agccggctac cgccaccggg atgaagattt cataagccag tgggacgctc
     1861 gccacctgtg tgcgctgttt aggcatacgt atggtcaggt aaacagccag cacaatgcca
     1921 atggtcaaca aaagaaaatc gggaatactg aaattggtaa aacggagcga ctgtgaaagt
     1981 ggcaggtcta cgaacagcct tgagatccag ccccccagcg caccgctggc gaagaccagc
     2041 gcgcacccga tgaacatagc cccgatggag cgagaaaaaa aggtgaagga gccgatgacg
     2101 gctgaaaaac ccagcccaat gaccggcgcc atgaacggcg ccagtaaagc cccaaggatg
     2161 aagatcgcgg gactgtcgag caggatggcc agggtgataa ccagaccagc caccagagaa
     2221 aacaaaaaga attcaacccc aggcaccagg cgctgggcaa tttcgccgag gtaaagggcg
     2281 cgttcacttt ttccccgtgg gaccagtaat ttacgcgcgc gccgccggcg gctctcatcg
     2341 atacggcttg gcggaattac aacacctggt tcctgtgagt aatcgctcat tgttatcgcg
     2401 ctgtttccaa tctaaacaag tggaaaaaaa atcccgccgc gcgcaaaaaa agtgctgccg
     2461 gcgcggaggt ggtactcggt ttgcccttat ggtggcggag ctctctggcg ttatggccag
     2521 tttggtcgca agaaatcatg acattattat aatacactca atattggcag taactaaggt
     2581 aaaatacccc tatggatcgc cacaatagac ttcaccttaa gcgaatctta tccctgcttt
     2641 tcatcattgg actgctgatg acaggctgct cgaaaccgac tgaccagccg ccagtaccca
     2701 gcgcaacacc gcttacacag gagccagtgg atacaccgac cgccatcccc acggctgctc
     2761 ccgcgcgtat gctgctgctg gatccgtttg ccgcggctac agctgagtta aacgcatacc
     2821 tcgccgggtt cgctgagcag catgacctca cgttggaaac ccaggcctca cctgaactgc
     2881 ccgcgcttgg tgctgaaacc aaagttgtcc ttctattggc agaactcccg ggactggacg
     2941 agctgaccgc gacctcacct gaaacgcagt tcatccttat tggcaatacg gcgttgaacc
     3001 cggctggaaa cctttccgtc atccagacca gggagtatga tctgaccttt atggcgggat
     3061 acctcgccca aagcattgcc tgggactggc gcactgccgg cctgattccc agcgacacgg
     3121 ccttaagcga gccaaaaacg aacgccttca ttaacggtgc gcgttatctg tgcggggtat
     3181 gcactccctt ctacgcccca attgtgtctt tccctatgct ggcccaggaa cccgcgcagg
     3241 cagacgccgc gacctggtcg ggacaggtga gcgtgttcgg gcagcacttt gtgaatactt
     3301 actttgttga ccccgctgtt gccaccgttg aaacgctgga cgggcttaaa ggtctggaac
     3361 cggccatttt taacgatgtg aaactgatag gaatctccac tacccccaac cccgagcgtt
     3421 ttacggcctt gatcggcttt gaccctcttc ctgcgcttga acaattgctt cctcagctga
     3481 tcgccggtaa tggcggcctc agcctgggag cgcaggtgaa gatcacttcc ttcaccgatg
     3541 atacagtcat caccccggcg aaaattgaca acttcaaccg cgtggccgct gacctggcta
     3601 ccggaatgat tgtccccctc agcatcccct aatacgcttc caacgttctg cagcggccaa
     3661 acgcaggatg ccgctctttt gcgacaggtc catcaggctc gccttgcctg gttgaggtag
     3721 ttgataactc ccacgaggct gctgatggtc aattaccaca gtgaatagcc ttgcgggttg
     3781 tttttacctg ttgagtgctt gtatttgaca ggcagaatca ggcaggttgc aagcgagaaa
     3841 cgaccaccta cccgaaaaca cggcaagagc gaaagataaa ccgttatact gatggtgaaa
     3901 taagtcattc aaggaggcag cagctgtgaa cgttgagata aaaatttcca aagacatcac
     3961 tgaaccatac gcggtcatct acaccaacgc gatcactgag gaagtacaga agattcgcag
     4021 cgttttagcg ttccaatcac cggtcatacc tgttagcgat gacgagaaaa tcgttgtatt
     4081 ggaacccgat gaaattttca tgatccgcac agagaacacg gaagttatcg tttacggcaa
     4141 agataccagg ttccggtcgg aaaaacggct gtacgagtta gaggaccttc tcgggaacgg
     4201 tttcttgcgc atctccaaat ccacgattgt caacctgaag aagattgatt atgtggaacc
     4261 gtccttcaat ggcatgatgg ccattcgatt aaaaaacgga gggaaagatt acatttcaag
     4321 aaaatatcta ccacaactta aaaactattt aggaatatag gaggtttgcg atgaaaaaat
     4381 ttttcgaaca ttttctaaaa gggatctcga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn gaagatcaac gcgcaattga agaagcagca agaatagccg ttctgctcta
     4561 actggcaaga agcctgtctt gtagcacaca ccccccgcgt aaggggggtg tttatttgaa
     4621 cgagggtttg cctgttccca tgaactctgg cgcggtggcg cttcctgggg cgttgatgcc
     4681 ctcgcgtttg agggctttcc acagggtgat gaagagaatc ttgatatcca gccacaatga
     4741 ctggtggtct acataccaca catccagctc gaacttctct tcccagctga gcgcgttgcg
     4801 cccgttcacc tgcgcccagc cggtgatgcc tggcagcact tcgtggcgcc gcgcctggtc
     4861 gggcgtgtag cgctccaggt aggcggtgag cagcggccga gggccgacca ggctcatctc
     4921 gcctttgagc acgttgaaga gctcgggcag ttcatccaga ctggaggagc gtaaaaaacg
     4981 cccgaaaggg gtgaggcgtt gttcatcggg cagcggtttg cctgaatcat cataagcgtc
     5041 gcgcatgctg cggaacttgt acaacttgaa ggggtgcccg cgcagccctg gtcgctcctg
     5101 gctgaagagc actggtctgc ccagcttgaa ccgcaccagc aacgccagta tcagcagcag
     5161 cggcgagagc aagataagca gcaaggcgct tgcgagtaaa tcgaagagac gtttgagaaa
     5221 acgcgtcatt ggtttgattg taccaaaatc cattgtcgaa cgcgcgttca ctgatgactt
     5281 tacgttataa tagagccact taacctcagg agcgctttcc atgatcatct ccattgaata
     5341 ctgcgttgtc tgacactaca ccccgcgcgc cgtcggtctg gcggctgaac tgttggaagc
     5401 ttacgaaacc aaaatcaaat cggtcaccct tattccgtcc agcggcggcg ctttcgaagt
     5461 gaacgtgaac ggtaatctca tttactccaa attgcaaacc ggtcgccacg ctgaagtggg
     5521 cgaggttgtg gctttattaa gaaaaaagat cgaggaaggg taaccatgac cagcaaaaaa
     5581 ggacgggttt tttcgggcgc caggccaacc gggcgccagc atattggcaa ctactttggc
     5641 gccatccgta attacgttaa gctacaggac gattacgatt gcatttattg cattgtggac
     5701 ctgcacgctc tgaccacgct ggataccacc cgcgatctcc ggcagaacac cctcgaaatg
     5761 gcctgggatt ggctggcagc gggcatggat ccgaaacgtt ccatcatctt cgtacaatcg
     5821 atggtgcgcc aggtgacgga actgcacacc atcctttcaa tggtcacgcc cttgggaaaa
     5881 cttaccgatc tgcccacgtt caaagaaaag gtgcgcgaaa acccgaagaa tatcaattat
     5941 gggctggtgg gctacccggt gctgatgagc gcggatatcg ccctttaccg cgccgatgtg
     6001 gtgccggtgg gaattgatca ggcgccgcac gtcgagtttg cgcgggaaac ggtgcgctcg
     6061 tttaacttcc gttacaacac caacgcgctg atcgaaccca aagagaaatt caccgaattt
     6121 cccaagatac tgggtattga cggtgtgaac aagatgagca agagcctcaa caacgatatc
     6181 gagcttgctg ccactgcgga agagactacc gcgcgtgtga tgcagatggt taccgatccg
     6241 gcgcgccagc gtcgcagcga ccccggcaac cccgatattt gcaatgtttt ttcgctgcat
     6301 aagatgttca ccccgcctga gaaggtagca gaaataaata tcgagtgccg tcgcgcgggc
     6361 attggctgtg tggattgtaa aagaattctg gcagccaaca tgaacgctta cttcgccccc
     6421 ctgcgtgaaa aacgccgtgt cttcgaaaat gaccccgagg gcacctggga catgctgcgc
     6481 gacggcgcgg cgcgcgccag cctgctggct gaagagacca tggtggatgt gcgcgcggcg
     6541 gtgggtctgc cagcgctgca gaaaggataa ccggcgatgc gcgctgtcat ccaacgggtc
     6601 aattacggcc gggtggtaat tgaagaccgc gaaaccgcga atattggcag cggcatggtg
     6661 attctgctgg gcgtaggccc gggtgatgac gagcaaaagt cagccgcgtt ggcgaagaag
     6721 atcgcccagc tgcgcatcat ggaagatgcg gaaggtaaaa tgaacctctc gctgnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccttct ctcaaaccct cgggcaggca
     6901 aattcgtctt accagcctct cgaactgcgc aggttgagca tccaagctga gcgcggaagc
     6961 gctggcgcag gtgctgcgcc ccatgcagga gctttttgac cctgccgaca cccctgagct
     7021 gctcgccggg ttggcttcgc ctgatgatgc ggcggtgtgg aaattggatg aagaccgcgc
     7081 cctggtgttg accaccgact tcttcacgcc ggtggtcgat gatccgtttg attacgggcg
     7141 catcgctgct gccaacagcc tctcagatgt ctacgccatg ggggctgcgc ctttcctggc
     7201 gctgaacatc gccgctttcc cacctgacct tgaccctgcc gtcagcggtg aaatttttcg
     7261 cggcggggcg acggtggcca agcaggcggg ggcagtgatt gcggggggac actccatcca
     7321 ggataaagaa cccaaatatg gcatggtggt agcgggtatg gtcgacgcgc aaaggatgtt
     7381 cagcaagggc ggcgccaagc cgggcgatcg cctttacctg accaagccgt tgggctttgg
     7441 cacaatcacc accgcgctca agcgccagat ggtgcgcgag gaagacctgg cagaagcagt
     7501 gagctggatg gtgcggctca accgcgaagc cagccgaata ggtatggatt gcggcgtgcg
     7561 cgcggcgacg gatatcaccg gttattcttt catcggtcac gcgctggaac tcgccaacgc
     7621 cagtggtgtg gggctgcggg tggcgtttga gcgcctgcct gtgctgccag ccgccttcac
     7681 atacgcatct caattcatct tcccgggcgg agcgtatgac aaccgttcgt attttcagcc
     7741 gtttgtagaa ttcagtcgga agctgccaga agaccagacc atgctgcttt ttgaccctca
     7801 aaccagcggc gggctgctgc tggccgttcc tgcccaacac tgcgcggatt ttgaagccgc
     7861 aatgcagcag ggtagcgctc cctgctggca ggtaggagaa gtgatcaaag gcagcgggat
     7921 catttttacc tgacgcaacc gaccgctcct cgctgagtcc ccgccctgtg ctaaaatccc
     7981 attatggttg aactgactga tcacctcaaa ggtattctgg ataccctgcc ggtgaaacct
     8041 ggttgttata ttatgaagaa cgcctccggc gaggtcattt acgtggggaa ggcggtcaac
     8101 ctgcgcggcc gtgtgcgctc ctacttccag gaacacgctg accacgaacg caaggtgctc
     8161 aagttagttt cgcaaatcgc cgaccttgag tggattgtgg tgggctcaga acttgaagcg
     8221 cttatcctcg agatgaacct gatcaagaag caccgcccgc gtttcaatgt gcgtctcaag
     8281 gatgacaagc gctatcctta tatcaaggtg cacctggcga gcgattttcc caaggttacc
     8341 gtgacccgtc agatggtcaa tgatggctct cgttattttg gcccctatac cagcgtgtgg
     8401 gcggtgcacc aaacgcttga cctgctgcgg cgcatcttcc cctatctcac ctgcgatcgt
     8461 gagatcaccg ggcttgacgc gcgaccctgc ctgtattacg acatcaaatt atgcagcgga
     8521 ccttgcatcg gcgcgatcga tcgcgccggg tacaaccagc ttatttcaga cctgtgcgcc
     8581 tttttggatg ggcgaacaga ccctatcgtt tcgcggctgc aacgcgagat gaacgatgcg
     8641 gcggaaagtt tgaattttga aaaagccgct ttactgcgag accaactgca gtcgatcgag
     8701 cgggtggtgg aaaaacaaaa agtcatcacc agcgaacaga tcgattcaga cgtgctggcg
     8761 attgcgcgcg cggataacga ggcttgcgtg caggtgtttt tcgttcgcaa tggtcgcctg
     8821 atgggacgtg agtattttgt catggaaggc actgaggaaa ttcccgacga acaggtgatg
     8881 gcggagttca tcaaacagtt ctataccgaa gctgcggtca tcccagaccg cgtgttgttg
     8941 cccaacgagg tgcaggaggc gcagatcatc cgccagtggt tgaacacccg ccgtggtggc
     9001 aataaagtgg agttacgtgt gccgcgccgc ggcaccgcgc atgagttgat gaaaatggct
     9061 accgaaaatg ccgttgaaac tttgcaggcg ctgcgcgcgc agtgggaggc ggacaagaac
     9121 aagcaaaccc aggcactcag cgagatccag caggcgctca acctgcccga gccgcccaac
     9181 cgcatcgagt gctacgatat ttccaacacg cagggcacaa cctcggtggg cagcatggtg
     9241 gtgttcgagc agggtacccc cagccgcgcg cattaccgtc agttcaatat caagacggtc
     9301 agcggtgcgg atgacttcgc cagtatggaa gaggtgctca accggcgttt caaacgctgg
     9361 caatctgcgc aagagaaaca ggggctgggc gcaaagcccg atccctcgtt cgctttgctg
     9421 cctgacctgc ttatcgtgga cggcggcaaa ggtcaattaa gcagggcggt gaaggttctg
     9481 gctgactata acctggcggg ggtggtgcca gtggtggggt tggccaagca gcaggaggaa
     9541 ttattccagc ctggcagcgg cagttctgtg gtattaccgc ggcattctca gggtttttac
     9601 ctgttgcagc gcatccgtga tgaggcgcac cgttttgcca ttaccgcgca ccgcaaagcc
     9661 cgtggcaggg tgggtgtggc gtcccgcctt gagagcatac cgggaattgg accggcgcgg
     9721 cggcgcgccc tgttgcagca gtttggtgat attgaacgca tccgccaggc ttcattgcaa
     9781 gagctgatgt cagtaccggg gattaacgaa gaactcgccc gggcggtgca agcggggctt
     9841 gagtgaataa ttgaagaatg cgtaaattag gggccggaaa ccaccggcag gcccttcgcc
     9901 atccaggcgt tgatgccgcc gctgatgctg gtggtacgct tcaaccccgc tgcgcgcagc
     9961 aggtcacgcg cttgcccgct gcgattaccg ctgcggcaga tgatgagcac gtcctgatca
    10021 accggcactt ctgccaggcg agatttgagc tcatccagcg ggatgcgcac tgctcctgcc
    10081 acatgcgcct ggttccattc ttctgctgtg cgtacatcca gtaagaacgc gccttcgttt
    10141 attctttgag cagcttcggt tacagataca gttgacggga gacctttggc tgcgtgacct
    10201 tctttggcga tggataacac tacgccaatg atcactgcca cgctgaccag gatcaatggg
    10261 agcagccatt tggtttgttt ttgttgtttt ttcttcgacg ttttggttgc cacgctccac
    10321 ctcaattaag ttttcagatt agatgttgga tttcatgata agtgacagag gtcaaccgcg
    10381 ttctgccagg gcttcgatgc cgttcatcag gctgtccaca atccccggtt cagaaatggc
    10441 gtgaccagcg tctggcacga tgtgcagttc agcctcaggc agatgctggc agaggttaat
    10501 cgccgaaacc accgggcaga tgatgtcata acgaccctgg gcgatccagc aggggagatg
    10561 agcgatctta tgcgcgttgt tcagcaagta gttatcatca ggcaggaaaa gcctgttcac
    10621 tatgtaatgg cactcgatgc gcgcgaaagc cagcagttca tcaggcgaca tgcgcggcgc
    10681 ttcacgcggc agcaggctga tgaggctttc ttcccagtgg taccagtgtt cggcggctgc
    10741 catctgcacc tcgcgctgga ttgaggtgag gcgttggtaa tacgccgctg gtagatcaga
    10801 gcgctcttcg ggagggatga acgattggaa acgctgccag ctctcagggt gaacgcgtga
    10861 agcgccatca acatacagcc agttgtgttc agcgcgcgta cctaaaaaga taccgcgtaa
    10921 caccaggcca cgcacgcgct ccggatactg ctgcgcgtag gctaaagcca gtgtgctgcc
    10981 ccacgagccg ccaaaaacga accagcgttc aacgttcacc gcttgacgca gtttttcgat
    11041 gtcttccact aatgcccatg tatcgttctg ttgtaattcg cctgacgagc tgctcttgcc
    11101 agcaccccgc tggctgaagt tgatgaccct gaaacgttca ggatgaaaga aacgccgata
    11161 gagtggctgt gcacccacgc caggaccgcc atg
//
LOCUS       DAOSPM010000058        11151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_5827_length_11151_cov_15.602289, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000058 DAOSPM010000000
VERSION     DAOSPM010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5827_length_11151_cov_15.602289"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PLW25_06240"
     CDS             <1..597
                     /locus_tag="PLW25_06240"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOS54123.1"
                     /translation="DMQDTYFLDVPNRGDNPLVLRTHTSPGQIRAMREIASLNPSNPP
                     PVRVIVPGMVFRYEQVDVRHETQFDQLEGLAIGEGITFSDLKGTLTDMARRLFGPSAR
                     ARFRASYFPFTEPSAEFDIQCFQCHGKGCGVCGGGWLEIGGSGMVHPVVLANGGYDPA
                     RYTGFAFGFGVQRIAMLRYGINDIRYFWENDLRFLEQF"
     gene            684..3224
                     /gene="pheT"
                     /locus_tag="PLW25_06245"
     CDS             684..3224
                     /gene="pheT"
                     /locus_tag="PLW25_06245"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOS54124.1"
                     /translation="MKAPLSWLKDFVEIDLSLVELAHRLTMAGLEVESIQLIGLPLPP
                     AGSTTAKVSGLTWEPEKIVAAAVTEVMPHPNADRLVLCRLEDGQQEHIVLTGAPNLFE
                     YKGKGPLPQPLKVAYAKEGAMLYDGHQEGYVLTTLKRTKIRGVESYSMICSEKELGIS
                     DEHEGIMFLDDAASVGTPLVELLGDAILEVNLLPSFARCASIFGLAREISAITGKALK
                     TPRFTSPPDIGKATYAELEITDPVLNPRFVLGLVRGVNIHPSPLWVQQRLKLAGMRPI
                     NNIVDATNYAMLELGEPLHAFDYDVLCKRAAGKQVKIITRAAGKDEKITTLDGVERTL
                     DEGTVLVCDEAGILSLAGVMGGLETEVTASTRNILLEGAAWNMISTRRTAKAQNLPSE
                     ASYRFIRGVHPAMAETGVRRGLELMEHWADGAVDPLMVDAYPLPPKTSVVDITPGDVK
                     RWLGLDLSAPRIAELLSRLEFKVQIDSDLLHITCPEHRLDIGSGITGKADLIEEIARV
                     HGYDNIPETRISDRLPLQLGNSPLKKTDLTRDLLVALGLQEVISYRWTTPEKEGRRLP
                     KGAEADNKPYLRISNPLAYEKAFLRHSVLASLLDTAERNSRQRATLALFEIGPIFLHS
                     EDPSGLPDELSRLGILLAGQRNPSGWQPADNSTMDFYDLKGLLEDLMAGLHITEANFS
                     QGEHPGYHPGKCAIIRVGSQQVGVCGEIHPAVRERYHWGDTFQYPVLAAELDMDLLIE
                     LIPEQRHTTDIPNFPAVVEDLAFVLDEATPAADVTALIWQTGGKILSELQLFDVFRGG
                     QLGSGKKSLAYRLTYQAVDRTLTDKETAQVRNRIIKRLEMELGARLRS"
     gene            complement(3306..4265)
                     /locus_tag="PLW25_06250"
     CDS             complement(3306..4265)
                     /locus_tag="PLW25_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS54125.1"
                     /translation="MHYRRLGQSGLKVSEISLGAWITFGNQIDEKTAISLIKEAFDQG
                     INFFDNADMYANGQAELVMGKAIADMPREELVISSKVFWPTMKGPNGRGLSRKHITES
                     IHASLKRLGTEYVDLYFCHRYDPDTPVEEVVRTMTDLVHQGKILYWGTSEWEASQIMQ
                     AYGAARQFGLIPPSMDQPQYNLFHRKRVEDEVMPVARELGLGLTTFSPLYYGVLSGKY
                     NNGIPKGSRASLEDYGWIRDRITPERIQVVKCLTEIASDLDITPAQLAIAWVLRRKEV
                     SSVITGATRLEQLDENLGASLAYERFTELDIDRIFACLGAHQE"
     gene            complement(4266..>4871)
                     /locus_tag="PLW25_06255"
     CDS             complement(4266..>4871)
                     /locus_tag="PLW25_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOS54126.1"
                     /translation="QAADRLTRAGIPLGNQSVLLAGVNDCVHIQRDLVQKLVRIRVRP
                     YYLYQCDLVEGAGHFRTPVAKGIEIIEGLRGHTSGFAVPTFVVDAPGGGGKIPVMPNY
                     HLSSSDHKVILRNYEGYITTYEEPQDYKAHDPKTCKYCQNKRSEPGQSGVSGLLDGEE
                     LAIKPENFDLLHNRGGGMHRLRADERKWKPLGIGAGQKEDK"
     assembly_gap    4872..4971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4972..5381)
                     /locus_tag="PLW25_06260"
     CDS             complement(<4972..5381)
                     /locus_tag="PLW25_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOS54127.1"
                     /translation="MTPTPFLSKRAPFFKDVPDEQWNDWRWQLSRRLNAVEDFDKIFT
                     LTESERQALSTDGLFRVDVTPYYVSLIDPDDPHDPIRRQIIPTAGEINAFTGMMEDSL
                     AEDRHSPVPGLVHRYPDRVLMLVTTQCASYCRYCT"
     gene            complement(5517..7451)
                     /locus_tag="PLW25_06265"
     CDS             complement(5517..7451)
                     /locus_tag="PLW25_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOS54128.1"
                     /translation="MNPMEEPINIFEQSSGIPREKKLQTWLIGRPLATADAPHQTIGK
                     LVGLAVFSSDAMSSVAYGPQELMLILAVAGAGALHLALPLVVGIAVLLIILTFSYEQT
                     IHAYTNGGGAYIVARDNLGELPAQVAGAALLTDYILTVAVSISSGVAQLVSAYPGLNS
                     FRVLIAVGLIFFVTIINLRGVKESGITFAIPTYFFLVLMLITVVAGFARYFTGTLGMV
                     VEPPLLEHEYLQPVTIFLLLKAFANGTTSLTGVEAITNGITAFKEPRAKNAGKTLLLM
                     SGILGVLLLSITFLSTKVGTIPSDSETIVSQLARTIYGSRGILYLLVISSTTIILIMA
                     ANTAFADFPRLGALQAADGFLPRQLTFRGSRLVYSSGIVLLAVTASLLVIAFRARVTG
                     LIPLYAIGVFLSFTLSQTGMARRWWKAGHLKPGDEVVESGSTLRYDKGWVHKMVINGF
                     GAFCTSIVAIIFAVTKFIDGAWIIIILVPLLVTIFFQIHRHYKKMARQLSLENPHTSR
                     MITRNRVIMPISGVHNGTLAGLQFAKSLSDDVTVVHVSIDPAETAKVKSKWERWGEGY
                     RLVILDSPYRLFLEPLLAYIEKISLILAPNEIITLVVPQFVPRHWWVNFLHMRAAETL
                     RKALLHSKKIVITEVPYQVD"
     gene            complement(7578..8273)
                     /locus_tag="PLW25_06270"
     CDS             complement(7578..8273)
                     /locus_tag="PLW25_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS54129.1"
                     /translation="MDKDPLILLIDDEPQILRALKTILSSGHFRVISAVTGEEGIALA
                     ASQLPDVIILDLTLPDMDGISVCEQVREWSTIPIIVLSVRDGERDKVAALDKGADDYL
                     TKPFNIEELLARIRVALRHSSQSIGNRETVIRAGRLTIDLARHLVSLDGTELKLTATE
                     FKLLAYIASHADRVLTHQAILTHVWGFEEADRLEYLRVYIGQLRKKIEVDPDAPVVLV
                     TDPGVGYRFKTGE"
     gene            complement(8266..9732)
                     /locus_tag="PLW25_06275"
     CDS             complement(8266..9732)
                     /locus_tag="PLW25_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HOS54130.1"
                     /translation="MKNSRNQPIKRIFQLVGNISMIGFTTFLLKQVESSLQIQLIALL
                     FLLPVLASTVLWGLTSGIIAALVSFLSFNYFFIQPYFTLNVHQTLDLITLIVFLIVAV
                     VVSQLIGQARRAVSLAEKREQESGQLYEVTSSLAGVTDIRETIRLLATRLFDTFHFDQ
                     IEISTWDLSSNELIIYQYPETARLETPADLVIHMSTARNDEGTLNLWFDQNKLTVEQT
                     RLLSAFCNQGALAIERVRLFRSENLAKVLEESDRVKTALLNSVSHELRSPLAAIKASV
                     TSLRGGAVPWETSARAELLATIDEETDALNLLVGNLLDMSRIEAGTLKPTKRWNSLIE
                     IIKGVEEKMRRQIGEHRIKYLVENELPLVPTDYVMMGQVFTNLFSNSNKYAPENTPIL
                     VKIGVAAGQMLVKVINQSPPVPEEHLEHIFEKFFRISETDKVTGTGLGLPICKGIVEA
                     HEGKIWAENTADGFTFNITLPLTLDGALPRLPKDVIDG"
     gene            9937..10974
                     /gene="hrcA"
                     /locus_tag="PLW25_06280"
     CDS             9937..10974
                     /gene="hrcA"
                     /locus_tag="PLW25_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOS54131.1"
                     /translation="MTELTDRQRMLLSLVIHEYVRSANPVGSKHVVEQFNLDMSSATV
                     RNELSTLTELGYLRQPHLSAGRVPTEDGYRLFVGNIVQETELPDSTRRTIAHQFYQMR
                     QDVEGWTRLAASILAHQSRAASLVTAPHPEQSRFKHLELIAARGRQVLMVLVLMGGEI
                     MQRFITLPEPVSQEQLSSAADRLNLIFSGQNIGEMSSDSSELVALEQEFYQIVLDEME
                     QADAMISGEVVLDGFTNVLAEPEFADSEEARRALRVLEEKTLLNDILSKTVLHGSSLG
                     GVQVLIGGEGIYDALSQCSIVLARYGTPGLATGTLGVLGPMRMPYGRTISIVRFLSGL
                     LSDLVNDTIIE"
BASE COUNT         2671 a         2978 c         2891 g         2511 t
ORIGIN      
        1 gatatgcagg atacctattt tttggatgtg cccaaccggg gagacaaccc cctggtgctg
       61 cgcacgcaca catcgccagg ccagatccgc gccatgcgcg agattgcatc cctcaaccca
      121 tccaaccctc caccagtacg cgtcatcgtg ccgggaatgg tctttcgcta cgagcaggta
      181 gatgtacggc acgaaaccca gtttgaccag ctggaagggt tggccattgg agagggcatc
      241 acctttagcg acctgaaagg cacgctcaca gatatggcgc gccggctctt tggtccatcc
      301 gcgcgcgccc gcttccgcgc ttcttacttt ccattcaccg agccatcggc tgaatttgat
      361 attcagtgtt tccaatgcca cggaaaaggc tgcggggtgt gcggcggcgg ttggctggaa
      421 atcggcggca gtggaatggt gcacccagtg gtattggcaa acggtggata cgacccggcg
      481 cgctacaccg gtttcgcttt cggtttcggt gtgcaacgta tcgccatgct gcgttatggg
      541 atcaacgaca tccgttattt ctgggaaaac gatttacggt tcttggagca gttttaaggc
      601 ccgtcaacgg tgagcggtga tcgggtcatc acaaacccaa tcccttagaa caactcgcga
      661 ttcacttcac ttggagaaaa cacatgaaag cccctttatc ctggttaaaa gacttcgttg
      721 aaattgacct ttcattggta gaactggctc accggttgac catggcgggt ttggaggtgg
      781 aaagtattca actcattggt ctgccgcttc cgccggcagg cagcaccact gccaaggtca
      841 gcggcctcac ctgggaacca gagaaaattg tcgccgccgc cgtgaccgaa gtgatgcccc
      901 accctaacgc tgaccgcctg gtgctgtgcc gcttggagga tgggcagcag gagcacattg
      961 tgctgaccgg cgcgccgaac ctgttcgaat acaaagggaa aggcccgctg ccacaaccgc
     1021 ttaaggtagc ttacgcgaaa gaaggcgcca tgctttacga tgggcaccag gaaggctatg
     1081 tgctcaccac gctcaaacgc accaagattc gcggggttga atcttattcc atgatttgct
     1141 cagagaagga actggggatt tctgatgaac acgaagggat aatgttcctc gacgacgcag
     1201 cctccgtggg cacgccgctg gtagaactac tgggtgatgc catattggaa gtaaaccttt
     1261 tgcccagttt cgcgcgctgc gccagtatct tcggtttggc acgtgaaatt tccgcaatta
     1321 ccggaaaggc gcttaaaacc ccgcgattca cctctccccc tgacatcggc aaagccactt
     1381 atgcagagct tgaaatcacc gacccggttc tcaatcctcg ttttgtcctg ggtttggtgc
     1441 gcggcgtcaa tatccatccc agcccgctct gggtgcagca gcgccttaaa ctggcgggca
     1501 tgcgccctat caacaatatc gtagacgcca ccaattatgc catgctggaa ttgggggaac
     1561 ctctgcacgc gtttgattac gatgtgctct gcaagcgcgc tgccgggaaa caggtgaaga
     1621 tcattacccg cgccgcaggc aaagacgaaa agatcaccac cctcgatggc gtggagagaa
     1681 cattagacga aggtaccgtt ctggtgtgtg atgaagccgg cattctttcg ctggcagggg
     1741 tgatgggcgg attggaaaca gaagtgaccg ccagcacgcg caacatcctg ctggagggcg
     1801 ccgcctggaa tatgatttcc acccgccgca ccgctaaagc ccaaaatctg ccctcagagg
     1861 cttcttatcg tttcatccgc ggtgtgcatc ccgccatggc agaaaccggt gtgcgccgcg
     1921 ggctggagtt aatggagcac tgggctgacg gcgcagtgga cccgttgatg gtagacgcct
     1981 accccttacc gccaaaaact tccgtggtgg atatcacccc gggcgatgtg aaacgctggc
     2041 tggggcttga cctgagcgcg ccccgcatcg ctgaattgct ttcacgcttg gagttcaagg
     2101 tgcagataga tagcgatctg ctgcacatca cctgccctga acaccgcctg gatatcggta
     2161 gcggtatcac tggcaaagcc gacctgatcg aggaaatcgc ccgggtgcac gggtatgaca
     2221 acatccccga aacccgcatc tccgaccgcc tgcctttgca gctgggcaac tcgcccctca
     2281 agaagaccga cctcacccgt gacctgctgg tagcgctggg gttacaggag gtcattagtt
     2341 accgttggac aacacctgaa aaggaagggc gccgtttgcc caaaggcgca gaagcagata
     2401 acaagcccta cctgcgcatc agcaacccgc tggcctatga aaaggctttt ctgcgccaca
     2461 gcgtgctggc cagcctgctg gacaccgccg aacgcaattc gcgccagcgc gcaaccctgg
     2521 ctttatttga gatcggcccg atcttcctgc actccgaaga cccctccggc ctgcctgatg
     2581 agttgagcag gctggggata ttgctggctg ggcagcgcaa cccgtcaggc tggcagcctg
     2641 cggataacag cacgatggat ttctatgacc tcaaaggtct tcttgaagac ctgatggcag
     2701 gtctgcacat cacggaggca aatttcagcc agggtgaaca cccggggtat caccccggaa
     2761 aatgcgccat catccgtgtg gggtctcaac aggtgggcgt gtgcggagaa atccatcccg
     2821 cggtgcgcga gcgctatcac tggggcgata cctttcaata cccggtcctg gcagccgagc
     2881 tggatatgga cctgctgata gagctcattc ccgagcagcg ccacaccacg gatatcccga
     2941 atttcccagc ggtcgtggaa gacctggctt ttgtgctgga cgaagccact ccggcggctg
     3001 acgtgaccgc gcttatctgg caaaccggcg gaaagatact ttctgagctg caactgtttg
     3061 acgtgttccg cgggggacag ctcgggagtg gaaaaaagag cctggcatac cgcctcacct
     3121 atcaggctgt ggaccgaacg ctgaccgata aagaaaccgc ccaggtgcgc aaccgtatca
     3181 tcaagcgcct agagatggaa ctgggcgcca gattaagaag ttgagatgag cagcctttac
     3241 aacataaagg atggcaaagc tgccatcctt tatgcgttct aataagacca gccgctgatt
     3301 tttgattact cctggtgtgc gcctaaacag gcaaagatgc ggtcgatatc cagctcagtg
     3361 aatctttcat aggcaagtga agcccccagg ttttcatcca gctgctcaag ccgggttgca
     3421 ccagtgatga cagaggaaac ctccttgcgt ctcagcaccc aggcgatggc cagctgcgcc
     3481 ggggtgatat ccagatcact ggcgatctca gtaaggcatt tgaccacctg gatacgctcc
     3541 ggggtgatgc ggtcgcgaat ccaaccatag tcttccagtg aagcgcggct gccctttggt
     3601 attccgttgt tatactttcc tgaaagcacg ccgtaataaa gcggtgagaa agtggtgagc
     3661 cccaacccaa gctcgcgcgc caccggcatc acctcgtctt ccacgcgttt gcggtgaaag
     3721 aggttgtact gaggttgatc catactgggt gggatgagcc cgaactgccg cgccgcgccg
     3781 tacgcctgca taatctgact ggcctcccac tccgaagtgc cccaatacag gatcttgccc
     3841 tggtgtacca ggtcggtcat ggtgcgcacc acttcttcta caggtgtgtc aggatcgtag
     3901 cggtggcaaa aatacaggtc cacatattca gtgcccaggc gcttgaggct ggcatgaatg
     3961 gactcggtga tgtgcttgcg tgaaagcccg cgcccattgg gtcctttcat cgtcggccag
     4021 aacactttgc tcgagatgac cagttcttct cgcggcatgt cggcaatcgc cttgcccatc
     4081 accagttctg cctgaccatt ggcgtacata tcagcgttgt caaaaaagtt gattccctgg
     4141 tcaaatgctt ctttgatcag gctgatggcg gttttctcat cgatctgatt cccaaaggta
     4201 atccacgcac ccagtgatat ttcactgact ttcaaccccg attgaccaag cctacgataa
     4261 tgcatttact tatcctcctt ctgtccagct ccaattccca gcggtttcca cttccgttcg
     4321 tcggcgcgca ggcggtgcat ccctccgccg cgattatgca gcaggtcaaa gttttctggt
     4381 ttaatggcca gctcttcgcc atccagcagc ccgctcacac cagattgccc gggctcgctg
     4441 cgtttattct gacaatactt gcaggttttt gggtcgtgcg ccttgtaatc ctgcggttct
     4501 tcataggtgg tgatgtagcc ttcgtagttg cgcaaaatga ccttgtgatc ggaagaactg
     4561 aggtgataat tgggcatcac cgggatcttg ccaccgccgc caggcgcatc taccacgaag
     4621 gtgggcaccg cgaacccaga ggtatggcct ctcaatcctt caatgatctc gatgcctttc
     4681 gccaccggcg tgcggaagtg accggcgcct tccaccaggt cgcactggta gaggtagtac
     4741 ggccgtacgc gaatacgcac caacttctgt accaggtcgc gctgaatgtg cacacaatcg
     4801 tttacgcccg ccagcaacac ggattggttg cctaatggaa taccggcacg ggtcagccgg
     4861 tcagccgcct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtgcaatag
     4981 cggcaataag aggcgcactg cgtggtgacc agcattaata cacggtcagg gtagcggtgt
     5041 accagcccgg gcaccggaga gtggcggtct tcagccaacg agtcttccat catgccggta
     5101 aaggcgttta tttcgccggc ggtaggaatg atctgtcggc ggatggggtc atgcgggtca
     5161 tcaggatcga tcagggatac atagtagggg gtcacatcca cgcggaaaag gccgtcggtg
     5221 ctgagcgcct ggcgttcgct ttcagtgagg gtgaagattt tatcgaaatc ctcaactgcg
     5281 tttaaacgcc ggctcaattg ccagcgccag tcgttccatt gttcatcagg tacatctttg
     5341 aaaaagggag cccgtttaga gagaaaggga gtgggtgtca tacatcctcc ataacctggg
     5401 ttgaactgaa gttttcacaa ttcattttaa gggctaatgg caggatggtc aacatattga
     5461 tttaaccacc tgccacctgc tcgggtggca gccgcagcgg ctgatttgag aagcggttaa
     5521 tccacctggt aaggcacttc agtgatcacg atcttcttgc tatgcagcaa cgctttacgc
     5581 aaggtttctg cggcgcgcat atgcagaaaa tttacccacc agtgacgcgg aacaaattgg
     5641 ggcacaacca gggtaatgat ctcattcggc gccagaatca gcgatatttt ttcaatatac
     5701 gccagcaagg gttcaagaaa caaccggtag ggtgagtcaa ggatcaccag gcggtaaccc
     5761 tcgccccagc gctcccattt gctcttcact ttcgctgttt ccgcggggtc aatagaaacg
     5821 tgcaccacgg taacatcatc agataaagat ttggcaaatt gcagccccgc gagtgtgccg
     5881 ttatgcaccc cgctgatagg cataatcacg cggttgcggg tgatcatgcg gctcgtgtgt
     5941 gggttttcca atgaaagctg gcgggccatt tttttataat gacgatgaat ctggaaaaaa
     6001 atggtcacta aaagcggaac gaggatgatg attatccagg cgccatctat gaacttggtt
     6061 accgcgaata tgattgcaac aatggaagtg caaaaagcac caaacccatt gatcaccatt
     6121 ttatgcaccc aacccttgtc atagcgtagc gtagaccctg attccacaac ctcatcgcct
     6181 ggcttcaggt ggcccgcctt ccaccaacgc cgcgccattc ctgtttgaga caaggtgaag
     6241 gaaaggaaaa ccccgatagc gtagagcggg atcaggcctg tcactctcgc tctgaaggca
     6301 atgaccaata atgaagctgt aaccgccagc agcacaatac cactggaata caccaaacgg
     6361 cttccgcgga aggtgagctg gcgcggtagg aatccatcag cagcctgtaa cgcgccaagc
     6421 cgcggaaaat cggcaaatgc cgtattcgct gccatgatca agataatcgt tgttgagctg
     6481 atgacaagca aataaaggat tccccggctg ccataaatag ttctcgccag ttgcgagact
     6541 attgtttcgc tatcagaagg aatcgtccca acctttgtgc tcaaaaacgt gatgctcagc
     6601 agtaacactc caagtattcc tgacattaaa agtaacgttt ttcccgcgtt cttcgctctt
     6661 ggttctttaa atgccgtgat tccgttcgta attgcctcaa cgcccgtgag cgaggtggtg
     6721 ccattggcaa aagccttcaa gaggagaaaa atggttacag gctgcaggta ttcgtgttcc
     6781 agtagcggtg gttcaacgac cattcccagg gtgccggtaa aataacgcgc aaaaccggca
     6841 acgaccgtga tcaacatcaa caccaggaaa aagtaagtag gaatggcaaa ggtgataccg
     6901 gactccttca ccccgcgcag gttaatgatg gtgacaaaaa agatcaaacc caccgcaatc
     6961 agcacgcgaa aagagttcag acccgggtag gcagaaacaa gttgggctac ccctgatgat
     7021 atcgatactg ctacggtgag aatataatct gtcaacaaag cagcgcctgc gacctgagcc
     7081 gggagttcac ccaggttatc gcgagcaaca atgtatgcac ctcccccgtt tgtgtaggca
     7141 tgaattgtct gttcatatga gaacgtgagt atgatgagaa gaacagcaat ccccaccacc
     7201 aacggcagag caaggtgcag cgctcctgct cccgccacag ccaggatcag cattaattcc
     7261 tgaggaccat aagccacaga agacatcgcg tcagaagaaa agaccgccaa cccaaccagt
     7321 ttcccgatcg tttgatgagg ggcgtcagcc gtcgccagag gacggcctat aagccaggtt
     7381 tgaagtttct tctcacgcgg tatgcccgag gattgttcaa agatattgat aggttcttcc
     7441 attggattca tttctttatt gttcccaatt cctgcttaaa aaataaaatt gactcaccat
     7501 ttcagaggta agtctacata tcaggcataa ggattaacta agagtcttgg gcaataaata
     7561 aagaaaaaat aaaggcttta ctcccctgtt ttgaacctgt agcccacacc agggtcagta
     7621 accaacacca ctggagcgtc aggatccact tcgatctttt ttcgcagctg gccaatatag
     7681 acccgcaggt attcaaggcg gtctgcctct tcgaaccccc atacatgggt cagaatagcc
     7741 tgatgggtca agacacggtc agcgtgcgaa gctatgtatg cgagcaactt aaattcagtg
     7801 gctgtgagct tgagttcagt gccatccagg ctgaccaggt gacgcgccag gtcaatcgtc
     7861 agtctgccag ccctgatgac cgtctctcta ttaccgatgg actgggacga gtggcgtagc
     7921 gcaactcgaa tgcgcgccag cagttcctcg atgttgaaag gtttggtgag gtaatcatca
     7981 gcgcctttat ccagagcggc aaccttgtct cgctcaccat cgcgcaccga aagcacgatg
     8041 atgggtatgg ttgaccattc tcgtacctgt tcgcataccg agatcccatc catgtccggc
     8101 agcgtcagat ccaggataat gacatcaggt agttgagagg ctgccagcgc gatgccctcc
     8161 tcgccggtca cagccgaaat gacgcgaaaa tgcccagagc tgaggatggt cttcaatgcc
     8221 cgcagtattt gcggttcatc atcaatcaac aaaattaagg ggtccttatc catcgattac
     8281 atcctttggt aatcgcggca aagcgccatc cagggtgaga ggaagggtaa tattgaaggt
     8341 aaacccatct gctgtgtttt ccgcccatat tttaccttcg tgcgcttcga caatgccttt
     8401 gcagatgggc aaccccaaac cggtgcctgt aaccttatcg gtctcggaaa ttcgaaaaaa
     8461 tttttcaaag atgtgttcca ggtgctcttc gggcaccggt gggctctgat tgatcacttt
     8521 aaccaacatc tgtccagcag ccacaccaat tttaacaagg atgggagtat tctctggggc
     8581 gtacttgtta ctgttactga aaaggttggt gaaaacttgg cccatcatta cataatccgt
     8641 cggcaccagg ggtagctcat tctctactaa atatttaatc cggtgttctc cgatttgcct
     8701 tcgcattttt tcctcaacgc ctttgattat ttcaataagc gaattccagc gtttggtcgg
     8761 tttcaacgta ccggcttcga tacgcgacat atctaataaa ttgccgacaa gcaaattcaa
     8821 cgcgtctgtt tcttcatcga tggtggccaa taattccgcc ctggctgaag tctcccatgg
     8881 aaccgcgcct ccccgcaggc tggtaacgga ggctttaatc gccgccagag gagagcgcaa
     8941 ctcgtgagag accgaattta acaaggcggt cttcacccgg tcgctttctt ccaacacttt
     9001 agccaggttt tcgcttcgaa aaaggcgaac cctttcaata gccagggcgc cctggttgca
     9061 gaacgctgaa agaagccgcg tttgttctac agtgagttta ttttgatcga accacagatt
     9121 cagtgttcct tcgtcatttc tggcggtgct catatggatg accaggtcag ccggggtttc
     9181 gagcctggcg gtttcagggt attgataaat gatcagctca ttggatgaca ggtcccaggt
     9241 ggaaatttca atctggtcaa aatgaaatgt atcaaaaagt cgggtggcga gcagtcggat
     9301 ggtctctcga atatccgtca ccccagctaa agaagaagtt acttcgtata attgcccaga
     9361 ttcttgctct cttttttcag ccaggcttac cgcccttctc gcctgcccaa tgagctggct
     9421 gacaaccacc gccacgatca aaaaaacgat caatgtaatc aagtccagcg tttgatgcac
     9481 attcaaagtg aaatacggct ggatgaaaaa ataattgaac gacaggaatg aaacaagcgc
     9541 ggcgatgatc ccggaagtta acccccacaa gaccgtggaa gccaaaaccg gaagcaggaa
     9601 gagcaaggca atgagctgaa tctgcaggct ggattccacc tgttttaata gaaaagtggt
     9661 gaacccaatc atgctgatat tacccacaag ttggaatatt ctctttatcg gttggttgcg
     9721 actgttcttc aaggtgagct ctccccctca tcttaccaga ttatcagctg ggtgcagcaa
     9781 gcattcaacg gattaaagcg gtaaacgcgt gaatttgcag gccatattaa tcttttatta
     9841 gaacgcccca ggcttccttg actcaacttt tccggcatgt tatcattgaa gccagtgaat
     9901 tagcactcag gcttgtcgag tgctaaaaag atgaaaatga ctgaactgac tgaccgccaa
     9961 cgcatgctgc tttctctggt aattcacgag tacgtccgct cggcgaaccc ggtgggctct
    10021 aaacacgtcg tggaacagtt caacctggat atgagctccg ccacagtgcg caacgagctt
    10081 tccacactca ccgaactggg ttacctgcgc caacctcact tatctgccgg cagggtgccc
    10141 accgaagatg gctaccgcct gtttgtgggc aatattgtgc aggaaaccga gttaccagat
    10201 tctacacgcc gcaccatcgc gcatcagttc tatcagatgc gccaggatgt agaaggctgg
    10261 acccgtctgg cggcttcaat actggcgcat caatcacgcg cagcctctct tgttaccgct
    10321 cctcaccccg agcaatcgcg cttcaagcat ctcgaactga tcgccgcgcg cggtcgccag
    10381 gtattgatgg tgctggtgct gatgggcggt gaaattatgc agcgctttat tacccttccc
    10441 gagccagtct cgcaggaaca gctttcaagc gcggctgatc ggttgaacct tatcttcagc
    10501 ggtcagaata ttggagagat gtccagtgac tccagtgagc tcgttgcact tgagcaggag
    10561 ttttatcaga ttgtgctgga tgaaatggaa caggctgacg cgatgatttc tggcgaggtg
    10621 gtgctggatg ggtttaccaa tgtgttggca gaaccagaat ttgccgactc agaggaagcc
    10681 cggcgcgcgc tgcgcgtgct ggaagaaaaa acgctcctga acgacattct atccaaaacg
    10741 gtgctgcatg gctcttcgct gggcggggtg caggtgctga ttggcggcga agggatttat
    10801 gacgctcttt cgcaatgttc cattgtcctc gcccgttacg gcacccctgg gctggcaacc
    10861 ggcaccctgg gtgtgcttgg ccccatgcgc atgccctacg gaaggaccat ctccattgtc
    10921 aggttccttt cagggttgct ttcagacctg gtgaacgaca cgattattga ataaccgcgc
    10981 cacgaaattt agaatagagg aatagatcat ggacgaagta aaaaaacaca gcggtaaaac
    11041 ccatcagcca gcaacagata aaagcgaaaa atacgcggca tcttctaaaa cggccatgct
    11101 gcaggaaatt gaacagctca agaaccagct ggcggaactg gaaaaagaat c
//
LOCUS       DAOSPM010000059        10727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_6138_length_10727_cov_15.347639, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000059 DAOSPM010000000
VERSION     DAOSPM010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6138_length_10727_cov_15.347639"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLW25_06285"
     CDS             complement(<1..409)
                     /locus_tag="PLW25_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOS54132.1"
                     /translation="MSEIYDNLMAAFAGESQANRKYLAYAKKAEEEGHTQVARLFRAV
                     AHAETIHALNHFRYAGEVRSTAENLNAAAAGEKYENTEMYPAFIENANKAGDKKALNS
                     FHWANEAEKVHEMLYREALASLGQPGEEYDYFIC"
     gene            587..1024
                     /locus_tag="PLW25_06290"
     CDS             587..1024
                     /locus_tag="PLW25_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOS54133.1"
                     /translation="MKVSDFTVDFRSGIPAYIQIVEQVQHKLVQGLLKPGEQLPTVRH
                     MAAHLRVNFNTIARAYRVLDNAGLISTQQGRGTYILERLEENRQQQLKRGALLTQARR
                     FFESLARQGYQPQEVEECLRDFLHSWKNTPGVSSSKIGMREME"
     gene            1146..2087
                     /locus_tag="PLW25_06295"
     CDS             1146..2087
                     /locus_tag="PLW25_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS54134.1"
                     /translation="MQHAIVIEHLSHRFNSRYALKDLSYEVRQGEIFALLGPNGAGKT
                     TTIRLVNGLYTPTSGRINVLGMDSVRQGSQIRQQTGVLTETSALYERLSARENLLFFG
                     RLYGVPESLLLKRTDEILQFFGLKNRESERAGAYSKGMKQRLALARAFLTHPRILFLD
                     EPTSSLDPESTQQVHELIESLCRQEENTVFLCTHRLEEAERLADRVAILNSGRLLALG
                     SLPELTQQFNHGLWVEVDLLQPLQNDPSSLRVRGVIDVQVSERKIRAQVAEEEIIPSL
                     VATLVAQKAQIVSVQPQKVTLEEIYFKLQSQAQEGAV"
     gene            2084..2914
                     /locus_tag="PLW25_06300"
     CDS             2084..2914
                     /locus_tag="PLW25_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOS54135.1"
                     /translation="MNWKTILTIAKKDIKEASQNKAVWLPILIVPAIFVLIIPAALIV
                     GFSSAGTALESPLRDPDFAMFLNRMPPFMIELIRGLNEVQSGIVLVLGYLFAPFFLIM
                     PLMFSTVIAAESFAGERERKTIEALLYTPATDAELFIGKVLAGFIPAVLVTLVSFAGY
                     TIVVNATAYPVMQRLWFPLPSWYPLVLWVSPAISLLGIAFTVLISAKTQTFMGAYQTS
                     GSLVLVVLALLAGQATGVVYLSVLVGLLIGLAIAVVDVILFSFAVRAFNREQLLTATS
                     "
     gene            3070..4059
                     /locus_tag="PLW25_06305"
     CDS             3070..4059
                     /locus_tag="PLW25_06305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HOS54136.1"
                     /translation="MISNIPLLITYALSLIVMIGLPVLLAFLVVRRFNVSWWVVLAGV
                     LTFLASQALRIPLVTGINTLFSKGILSVPAAQWVPVVNAVLAGLLAGICEETARWLGF
                     KLMQKKTERYGSAFALGVGHGGAESILIAVLSIAIPIFTVLFYNPGAQIAKGVSTNDV
                     QYMLFQISRFWLDPWHAGLLPGVERVIAISTQIVLATLVWKSIADRSFTWFGLAVLYH
                     MVIDALAVFLRSKGWSLWSIEGVLAIFLLLNIYLLYRFWKEEAEIEDEVQTIDGEEDG
                     YDEDRDEDDEDNDEDPSGEEQAQVEPLAVESDEQPFSEDADEEAAGTVEEDPR"
     gene            complement(4105..6435)
                     /locus_tag="PLW25_06310"
     CDS             complement(4105..6435)
                     /locus_tag="PLW25_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOS54137.1"
                     /translation="MDFSPNTSERLLNITLALAQYPILAGRIRARMREELFTRQITDA
                     KRFEARVHENAIKSQGLEGVSNPYSEESSDLWERRKSIVRDHLTDLLFSRHLTFELLE
                     SLIKEVLNERGISSDEMLLSINPELAPLDLVFEQALLIEGMPEEKRARYIARLEESKV
                     VLIRTLISDQLRYINIAKEWLSIQDLAEIRRRKIGGGRIGGKSAGMILAQHILRNNSD
                     LSDLPCLSMPESFYVGSNEFYTFMSINNLLKWNDQKYKTETEMRDDYPHIEEEFLKGT
                     FPPDILDQLQTLLIKIGKQPLIVRSSSLLEDNFGTSFAGKYESVFCPNQGSLAENLKA
                     LTRAIAQVYASTLNPNALLYRRSKGLLDYDERMALLIQWVEGDQYGDYLMPHAAGVAF
                     SHNLYRWSPQIRSEDGFIRLVWGLGTRAVDRVGNDYPRLIALSHPLLRPSNDPRSIRR
                     YSQQYVDLIDLKKNQFTTMPIHQVLAADYPPLRYLAQVDEDGYFESLRSTLISNVQNL
                     VLTFDVLIQRTPFAERMKTILKSLEQAYHAPVDVEFTVNLTENSVGSPQLCITILQCR
                     PQGFLIETEVERIPPDLTPEEILFSTDFMVPQGKVSAVDWVLYVQPEAYFALKTNAER
                     AALARTIGKLNDVLKDESFICIGPGRWGSSNADLGVPISYGDIYHARALVEMAGEHCG
                     LPPEPSLGTHFFQDLLESQIYPLALQLDDPATVFNRSFFDHAPNRLNELLPEAAGFAG
                     CLRVLRISDSYPGQTLRLIMNGEIGRAAGFLVNTQE"
     gene            complement(6501..7628)
                     /gene="dnaN"
                     /locus_tag="PLW25_06315"
     CDS             complement(6501..7628)
                     /gene="dnaN"
                     /locus_tag="PLW25_06315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOS54138.1"
                     /translation="MKVTVTQQHLAHGLSIVSRAVSPRTTLPVLANVLLATDEGRLRL
                     SATNLELGITCWIPAQIEEEGSITLPSRTLVDLVSTFPPDTVHLTLNDRTQTLNLVCN
                     SSVHDIKGIDAQDFPPMPVTDPEEGFDLNLADFKDMIQQVAFAASTDEARPVLQGVKM
                     EVVENHFTLAATDGFRISVRKESLSNPVEQELSVIIPARAMNELARIFSEVDKSVRMV
                     VPQGRGQVIFRLKDAELVSQLIDGNFPDYKAIIPKSAKTKTIISTPAFLKACRQAEVI
                     AREGNNVVRLDIRPEEEGPGKVEINAVTEETGQSDISVEAHIEGSGLLIAFNVRFLRE
                     VLDVIHSPNVALETNANNSPGVIRPMGEEDFVHVIMPMHLG"
     gene            7797..8375
                     /locus_tag="PLW25_06320"
     CDS             7797..8375
                     /locus_tag="PLW25_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS54139.1"
                     /translation="MAKKPVAVQKWEYTPLTPARWEDFELLFGPNGACAGCWCAWFRM
                     TSREFSASKKEGHKELMRTIVHSGVEPGLVAYADGVPAGWVALGPRESYKRLATSTVL
                     GPVDDQPVWVLPCFFIHRDYRCQGGMEKLLMAAIDYAKSKGVKILEAFPVEAEGKVSP
                     VHLFTGKASVFRRLGFKQVAMRENRPILRLAL"
     gene            complement(8460..8541)
                     /locus_tag="PLW25_06325"
     tRNA            complement(8460..8541)
                     /locus_tag="PLW25_06325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8505..8507),aa:Leu,seq:caa)
     gene            8594..9670
                     /gene="ychF"
                     /locus_tag="PLW25_06330"
     CDS             8594..9670
                     /gene="ychF"
                     /locus_tag="PLW25_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOS54140.1"
                     /translation="MRLGIIGLPQSGKTTLFNALTRATQPTGASGKIEIHTAVVDVPD
                     RRVDVLSAMFKPKKTIYAKVTYVDIAGLDGSASKSGISGALLNQLTQMDGFIQVVRAF
                     TDENVAHVHESINPQRDILNMDSEFVLNDLIAVERKLERLEEEKKKGAGREKGLIERE
                     HALFTRLQEILAGEVPLRDVEISEEDEKLLSGFGFLSRKPMMIVLNLAEGQAQPVVDY
                     THQNSRVISLQGKLEMDIAQLSADEAALFLAEYGIEEPSLSRMIRLSYDLLGLHSFFT
                     VGPDECRAWTVKRGATAPEAAGEIHTDLQKGFIRAEVVHYDDLVALGGMNEAKAHGKL
                     RLEGKEYVVKDGDVLNILFNVTKR"
     gene            complement(9716..10528)
                     /gene="surE"
                     /locus_tag="PLW25_06335"
     CDS             complement(9716..10528)
                     /gene="surE"
                     /locus_tag="PLW25_06335"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOS54141.1"
                     /translation="MKKPQILLTNDDGIHSPGLWAAARKLAKLGYVTVAAPREQHSGA
                     GRSVPAWTDGEVKPTTLQIGDQEWTCYAVGGSPAQSVQYGVFAILQQKPDLVVSGINY
                     GENPATDVSLSGTVGAGLEAASYGIPALAVSLELENEDYLGYSRATDFSTAAVFTRRF
                     AKLLLARQMPEDVMVLNVNVPVSATPDTPWRVTRLGKQAYFVPFLKQPEKAGAIPRID
                     ASRNPHPSDLGDPTTDVHVLLRQKMVSVTPLSLDLTSRVDLEELQRSFGTLD"
     gene            complement(10591..10664)
                     /locus_tag="PLW25_06340"
     tRNA            complement(10591..10664)
                     /locus_tag="PLW25_06340"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10628..10630),aa:Arg,seq:gcg)
BASE COUNT         2463 a         2754 c         3045 g         2465 t
ORIGIN      
        1 ggcagataaa gtaatcgtat tcctctcctg gttgcccaag cgaagccagc gcttcccggt
       61 acagcatctc gtggaccttt tcagcctcgt tcgcccagtg gaaactattg agcgcttttt
      121 tatcgcccgc tttattagcg ttttcgataa aagcggggta catctccgtg ttctcgtatt
      181 tttcaccagc cgcggccgca ttcagattct cggcagttga tctcacctcg ccggcgtagc
      241 ggaaatggtt gagcgcatgg atcgtttcgg cgtgcgccac ggcacggaaa aggcgggcca
      301 cctgggtgtg accctcttcc tcagcctttt tggcatacgc caggtacttg cggttcgcct
      361 ggctctctcc agcgaaggca gccatcaggt tgtcgtaaat ctctgacatt gttctctcct
      421 tatcaatgtt tgagtggtat gaggatgaat aaccccgcgt ttattgtaac ctgaaagacc
      481 gaagagaaaa gatgacgaaa ctcctgtttt actccctggg tggtgcatca ccggaaaaac
      541 aatgatacaa tagtaaaatg atacaatagg aagaggagat acgaatgtga aggtgagtga
      601 ttttacagtt gattttcgtt cagggatacc ggcttacatt cagattgtcg aacaggtgca
      661 acacaagctt gttcaggggt tgttgaagcc gggtgaacaa ctgcccacgg tgaggcacat
      721 ggctgcgcac ctgcgggtga atttcaacac catcgccagg gcttaccgcg tgttagataa
      781 cgccgggctg atctccactc agcagggtcg tggaacttat attcttgaac ggctggaaga
      841 aaatcgtcaa caacagctca aacgcggcgc gctgctgacg caagcgcggc gtttttttga
      901 gtcccttgcg cggcagggat accaacccca ggaagtggag gaatgcctgc gcgatttttt
      961 acacagctgg aaaaacaccc cgggtgtatc ttcttcgaaa attggaatgc gagaaatgga
     1021 atgaaaagat tagtgctaag caactccgga aatttgcgaa agaagtcaac cgggtgcccc
     1081 acttctgccc atcaggcggt tggggatgat catgtttcac gtgaaacata gagaaggatt
     1141 gacttatgca gcatgcaatc gtcattgagc atctaagcca ccgctttaac agcaggtacg
     1201 cgttaaaaga tctctcatac gaggtcaggc agggggaaat atttgccctg ctgggaccaa
     1261 acggggcggg gaaaaccacc accatccggc tggtgaatgg gttatatacc cccacctctg
     1321 gtcgtatcaa cgtgttgggc atggattcag tacgccaggg cagccaaata cgacagcaaa
     1381 caggcgtgct cactgaaacc agcgcgcttt acgaacggct cagcgcgcgt gaaaacctgc
     1441 tcttttttgg acggctttac ggcgtaccag aatcgcttct gttaaaaaga acggatgaaa
     1501 ttctacagtt ctttggatta aaaaacaggg aaagcgaacg tgcaggcgcc tacagcaaag
     1561 gcatgaagca gcggttggcg ctggccaggg cattcctgac ccacccgcga atcctctttc
     1621 tcgatgagcc cacctccagc ctggacccgg agtctacgca gcaggtgcac gagctcattg
     1681 aatccctttg ccgtcaggaa gaaaacacgg tttttctctg cacccaccgt ttagaggagg
     1741 ctgaacgcct ggcggaccgc gtggcgattc tcaacagcgg ccggctgctg gcgctgggaa
     1801 gcctgcctga actcactcag caattcaacc acgggttatg ggtcgaggtc gatttactgc
     1861 agcccctgca aaatgaccct tctagcctgc gcgtgcgcgg cgtgatcgat gtgcaggtca
     1921 gcgagcgcaa aatacgcgcg caggtagcgg aagaggagat tattccctcg ctggtggcga
     1981 cgctggtagc ccaaaaagcc cagatcgtga gcgtacaacc tcaaaaggtt acactggaag
     2041 agatttactt caagttgcag tcacaagccc aggaaggtgc ggtatgaact ggaaaacgat
     2101 cctcactatc gctaagaaag acattaagga agccagccaa aacaaggcgg tctggctgcc
     2161 tatcctgatc gtgccagcca tcttcgtgct catcataccg gcggcgctga tcgttggttt
     2221 ctcatccgca ggaaccgctc tggaaagccc cctgcgcgac ccggattttg ccatgtttct
     2281 taaccgcatg ccgccgttta tgatcgaact catacgcggg ttaaacgaag tacagtcggg
     2341 cattgtgctt gttttgggct atctgtttgc gcccttcttt ctcattatgc cgttgatgtt
     2401 ctcaacggtc atcgccgcag agtctttcgc tggtgaacgt gaacgcaaga ccattgaagc
     2461 gttgctttac acacccgcca ctgacgcgga gctgttcatc ggcaaggtgc tggctggttt
     2521 catcccggcg gtgttggtca cgctggtcag ttttgcgggt tacaccatcg tggtgaacgc
     2581 cacggcttat ccggtaatgc agcgcctgtg gttccctttg cccagctggt acccgttggt
     2641 tttatgggtt tcccccgcca tttctttgct ggggattgct tttaccgtgc tcatttcagc
     2701 taaaacgcag acctttatgg gcgcgtatca gaccagcggc tcactggtgc tggtggtgct
     2761 ggctttgctg gctggccagg ctaccggggt ggtctacctg agtgtgctgg tcgggctgtt
     2821 aattggactg gcgatcgctg tggttgatgt gattttgttc tcttttgctg tgcgcgcctt
     2881 caaccgcgag caactattga cagcgaccag ttgaggattg tttcacgtga aacaatcgcg
     2941 cgccccacaa gaaggtgttt tatggaggtt gaagaaggtg cgcctgatat tactgcgaaa
     3001 atcgactata cttgggcaat ccctcgcgca cggtctggcg accgcgtaat ctgaggaaag
     3061 gcatgttgca tgatttcgaa tattcctttg ctgattactt atgcgttgtc tttgatagtg
     3121 atgatcggat tgccggtatt gctggctttc ctggttgttc gccgcttcaa cgtatcgtgg
     3181 tgggtcgtgc tggcaggggt gctcaccttc ctcgcctcgc aagcgctgcg tatccctctc
     3241 gttactggta tcaacaccct tttttccaag gggattctca gcgtacctgc cgcgcaatgg
     3301 gtgcctgtgg tcaacgctgt cctggctggt ctgctggcgg gaatttgtga agaaaccgcg
     3361 cgctggctgg gatttaaact gatgcagaag aaaacggagc gctatggttc agcattcgcg
     3421 ctcggcgtgg gacatggggg ggcggagagc attttgatag cggttttaag catcgctatc
     3481 cccatcttta cagtgctctt ctacaacccc ggggcgcaga tcgcaaaagg cgtttcaacc
     3541 aatgatgtgc agtacatgct ctttcagata agtcgattct ggctcgaccc ctggcacgca
     3601 ggcttgctgc caggcgttga gcgcgtgata gccatctcca cgcagattgt tttagccaca
     3661 ctggtatgga aatcgattgc ggatcgttct tttacatggt tcgggctcgc cgttctctat
     3721 cacatggtta ttgacgcgct ggcggtattc ctgaggagta aaggttggag cctctggtcg
     3781 attgagggcg tactggctat cttcctgctg ctgaatattt acctgttgta ccgtttctgg
     3841 aaggaagaag cggaaatcga ggatgaggta caaaccatag atggcgaaga ggatggttac
     3901 gacgaagaca gggacgagga cgatgaagat aacgacgaag acccctctgg tgaagagcag
     3961 gcgcaggttg aaccattggc tgttgaaagc gatgaacaac cgttcagtga agacgctgat
     4021 gaagaagcag ctggtacggt tgaggaagac ccgcgctgag agcaaaaata ccctaaccgc
     4081 tccccattaa gggagcggtt ttttttattc ctgcgtattg accaggaaac ctgctgcccg
     4141 gccgatttcc ccgttcatga tcaggcgcaa cgtttgaccg gggtagctgt cgcttatgcg
     4201 cagcacgcgc aaacacccgg caaagcccgc cgcttcaggc agcagctcgt taagccggtt
     4261 tggcgcatgg tcaaagaaag agcggttgaa gaccgtcgcc gggtcatcca gctgcagcgc
     4321 cagcgggtag atctgagatt ccagcaggtc ctggaagaag tgcgtgccca gcgagggctc
     4381 aggcggcagc ccgcagtgct ctccagccat ttccaccagc gcgcgggcgt ggtagatatc
     4441 tccgtaactg atgggcacgc ccaggtcagc gttggaacta ccccatcggc ctggtccgat
     4501 gcaaataaaa gattcgtcct taagcacatc gttcaattta ccgattgttc tcgccaaagc
     4561 ggcgcgttcc gcgttggtct tcaacgcaaa gtaggcttcc ggctgcacat aaagcaccca
     4621 atccacagca ctgaccttgc cttgcggtac catgaaatcg gttgagaaaa gaatttcctc
     4681 gggggtgagg tctgggggaa tgcgttcgac ctcggtttcg atcagaaagc cctgcggtcg
     4741 gcactgcaag atggtaatgc ataattgcgg gctgccgacg ctgttttccg tcaggttgac
     4801 ggtaaattcc acatctaccg gagcgtgata agcctgctcc agcgatttca ggatggtttt
     4861 catacgttct gcaaaaggag tgcgctggat caagacatca aaggtgagca ccaggttctg
     4921 tacgttgctg atgagggttg aacgcaacga ttcaaagtag ccgtcttcat ctacctgggc
     4981 caggtaacgc agcggcgggt agtccgctgc caacacctgg tggatgggca tggtagtgaa
     5041 ctgattcttc ttcaggtcga tcaagtccac atactgctgc gagtaacgcc ggatggagcg
     5101 tgggtcattg ctgggccgca atagcggatg gctgagggcg atgagccgcg ggtaatcatt
     5161 accaacgcgg tcgaccgcgc gcgtgcccaa cccccaaacc aggcggatga agccgtcttc
     5221 actgcgtatt tgcggagacc agcggtacag gttgtggctg aaggctacac cggcggcgtg
     5281 tggcatcagg taatcgccgt actggtcgcc ctctacccat tggatgagca gcgccatgcg
     5341 ttcatcgtaa tccagcaatc ccttgctgcg gcggtagagc aacgcgttgg ggttaagtgt
     5401 ggaggcgtac acctgggcaa ttgcccgggt gagcgctttg aggttctctg ccaaagaacc
     5461 ctggttaggg cagaagacgc tctcgtattt gccggcgaaa gaggtgccaa agttgtcttc
     5521 cagcaggctg gaggagcgca caatgagtgg ctgtttgccg attttgatca gcagcgtctg
     5581 caactggtcc aggatatctg gagggaaagt gcctttaagg aactcttctt caatgtgcgg
     5641 gtaatcatcg cgcatttcag tttctgtctt gtacttctgg tcattccact tcaacaagtt
     5701 gttgatggac atgaaagtgt aaaactcatt cgaacctaca tagaaagact ccggcatcga
     5761 taaacaaggg aggtcgctca agtcgctgtt attacgcagg atgtgttgcg ccaggatcat
     5821 gccggcagat ttaccgccga tgcgccctcc gcctattttg cgccgacgaa tctcagccag
     5881 gtcctggatg gacagccatt ccttggcgat attgatgtag cgcaactgat cactgatcag
     5941 cgtgcggatg agcacaactt ttgattcttc taacctggca atgtaacggg cgcgtttttc
     6001 ttccggcatg ccttcgatca acagcgcctg ttcaaacacc aggtcaagcg gcgccagctc
     6061 tgggttaatc gataacaaca tctcatccga agaaatgccc ctttcgttca acacttcctt
     6121 gatcagcgac tccagcagct cgaaggtcag gtggcggctg aacaacaggt cggtaaggtg
     6181 atcgcgcaca atcgacttgc gcctttccca caggtcggaa ctttcttcgc tgtagggatt
     6241 gctgaccccc tcgagccctt gagatttgat ggcgttctcg tgcacgcggg cctcaaaacg
     6301 cttcgcatcc gttatttgcc gggtgaatag ttcttcacgc atgcgggcac ggatgcgccc
     6361 cgctagaatg gggtactgtg ccagcgccaa cgtgatgttg agcagccttt ctgaggtgtt
     6421 gggcgagaaa tccatcaatt atttctagaa aatattaaga accaaaaacc cctctccgca
     6481 aggagagggg cgtttggaga ttatccaagg tgcatcggca tgatgacgtg aacaaaatct
     6541 tcttcaccca ttgggcggat aacgccaggc gagttattgg cgttggtttc aagcgccacg
     6601 ttaggtgagt ggatcacgtc cagcacttca cgcagaaaac gcacattaaa ggcgatgagc
     6661 agaccactgc cctcgatgtg ggcttcaaca gaaatgtcac tctgcccggt ttcttcggta
     6721 acagcgttga tttccacctt gcctgggcct tcctcctcgg gtcggatatc caggcggacc
     6781 acattgttac cctcacgcgc gatgacttca gcctgtcggc aggctttgag gaaagccggc
     6841 gtggaaatga tggtcttggt cttggcgctc ttggggatga tggccttgta atcagggaag
     6901 ttgccgtcga tgagttggga aaccagttcg gcgtctttca gacggaaaat gacctgtcct
     6961 cgaccctgcg gtacgaccat gcgcacggat ttgtccacct cgctgaaaat ccgcgccagt
     7021 tcattcatcg cgcgggcggg gatgataacg gaaagttcct gctcaacggg gtttgaaagc
     7081 gattctttgc gcacagaaat gcgaaaccca tccgtggcgg caagcgtgaa atggttttca
     7141 accacttcca ttttgacccc ctgcagcacc gggcgggctt catccgtgga ggcggcaaag
     7201 gccacctgct ggatcatgtc tttgaaatcc gccaggttca ggtcaaagcc ttcctccggg
     7261 tcggtcactg gcatgggcgg gaagtcctgc gcgtcgatgc ccttgatatc gtgcactgag
     7321 ctgttgcaca ccaggttgag ggtttgggtg cgatcgttca gcgtcagatg gacggtatcc
     7381 gggggaaagg tgctgaccag gtccaccagg gtacgggaag gaagggtaat ggagccttcc
     7441 tcctctatct gagcaggtat ccagcaggtg atgcccagct caaggttggt ggcagaaagg
     7501 cgcaggcgtc cctcgtcggt ggccaacagt acatttgcca gcacaggcag ggtcgtacgc
     7561 ggagatactg cacggctgac gatgcttaag ccgtgcgcca ggtgttgttg ggtgacagta
     7621 accttcatga gcgccctctt ttcgctagtt ttgtatgctt gagtatacaa cgtataaagt
     7681 aaaaattcca gtaaaaaaaa aacgccaacc aataagtatt taaacctgga gtagaagttg
     7741 tataatcaga aagaacaata ttccgatcaa cccttttggt gagaaaggca gccgttatgg
     7801 caaaaaaacc ggttgcggtg caaaaatggg agtatacccc gctgactccc gcccgttggg
     7861 aggattttga gcttctcttt gggcccaatg gcgcctgcgc cggctgctgg tgcgcctggt
     7921 tccgcatgac cagccgggaa ttcagcgcgt cgaagaaaga aggtcacaaa gagttgatgc
     7981 gaacgatcgt tcactctggc gtggaacccg gcctggtggc ttacgccgat ggtgtgcccg
     8041 ctggttgggt ggcgctggga ccgcgtgaaa gttacaaacg cctggcgacc tcaacggtat
     8101 tgggcccggt cgacgaccag ccggtttggg tgctgccctg ttttttcatc caccgcgatt
     8161 accgctgcca ggggggaatg gaaaaattac tcatggctgc catcgattac gcgaaaagca
     8221 agggcgttaa gatccttgag gcatttccag tggaggcgga aggaaaagtg agcccagtcc
     8281 acctctttac cggtaaagca tctgtatttc gccgcctggg gtttaagcag gtggcgatgc
     8341 gagaaaaccg accgattttg cgcttagcgc tgtaattatt tctagaaaca aatccaacca
     8401 ggtggatttt tggaagaaaa aacccgctct tgagcgggga gaaaatgtta taatccttgt
     8461 gccgaaggtg ggaatcgaac ccacactccc gagggaacac gattttgagt cgtgcgcgtc
     8521 tgccagttcc gccactccgg cgataccgaa cagagtatat ctgactaatt cacaagggtc
     8581 aagggaaacg accatgcgtc taggaatcat cggattacct caatcaggaa aaacgacatt
     8641 attcaacgcg ctcacccgcg ccacgcagcc gaccggtgcc agcgggaaaa ttgaaatcca
     8701 taccgcggtg gtggatgtgc ccgataggcg cgtggacgta ctttcagcca tgttcaagcc
     8761 gaaaaaaacc atctacgcca aggtcacata tgtggatatc gctggattgg acggcagcgc
     8821 gagcaaaagc ggcatctctg gcgctttgct caatcaactg acacaaatgg acggttttat
     8881 ccaggtggtg cgcgccttta ctgacgaaaa tgtggctcac gtgcacgaga gcatcaaccc
     8941 gcagcgcgac atccttaata tggatagtga atttgtgctc aacgacctca tcgcggtgga
     9001 acgcaagctg gagcgcctgg aagaagaaaa aaagaaaggc gccggcagag agaaaggtct
     9061 gatcgagcgc gaacacgcgc tcttcacccg tttgcaggaa atcctggcag gtgaagtgcc
     9121 gctgcgcgat gtggagatca gcgaagaaga cgaaaagctg ctctctgggt ttggcttctt
     9181 aagccgaaaa ccgatgatga tcgtgcttaa cctggcggaa ggacaggcgc agcctgtggt
     9241 tgactacacc catcagaaca gcagggtgat cagcctgcag gggaaactgg agatggacat
     9301 cgcccagctt tcagccgacg aagcggcgct attcctggcg gaatacggta ttgaagagcc
     9361 cagtctcagc cgcatgatcc gcctgtctta tgacctgctg ggattacatt cttttttcac
     9421 tgttggtccg gatgaatgcc gcgcctggac ggtaaaacgc ggcgccaccg cgcctgaagc
     9481 ggccggcgag atccacactg acttacaaaa ggggttcatt cgcgccgaag tggtgcacta
     9541 tgatgacctg gtagcgctgg gagggatgaa cgaagcgaaa gcccacggaa aattgcgcct
     9601 ggagggcaag gagtacgtgg tcaaagacgg ggacgtgctc aatattctct tcaatgtcac
     9661 aaaacgctga agcgcaacga tacaaaaaca acctcctggc gaggttgttt tttcttcaat
     9721 ccagcgtgcc gaacgaacgc tgaagttcct ccaggtcgac gcgtgaagtc aggtcaagac
     9781 tgagcggcgt gacagaaacc attttctggc gcaggagcac atgcacatcc gtggtggggt
     9841 cacccaggtc ggagggatgg gggttgcgtg aagcatcaat acgaggaatg gcgcctgctt
     9901 tttcaggttg cttgagaaat ggcacgaagt aggcctgttt tcccagccgg gtaacccgcc
     9961 agggggtatc tggcgtggcg ctcacgggca cgttaacatt taataccatt acgtcttcag
    10021 gcatctggcg agcgagcagc agtttggcaa aacggcgggt gaacaccgcg gcggtggaga
    10081 aatcagtcgc gcgcgaatac cccaggtaat cttcgttttc cagctccagg gagactgcca
    10141 gcgccggaat gccgtaggaa gcggcttcca accccgctcc gaccgtgccc gaaagagaga
    10201 catctgtggc tgggttctcg ccataattaa tgcccgatac caccaggtcg ggtttttgct
    10261 gcagtatggc gaagacccca tactgcaccg actgagcggg agaaccgccc acagcgtaac
    10321 aggtccattc ttggtcacca atctgaagcg tggtgggttt tacctctccg tcagtccagg
    10381 cgggcacgct gcgacctgcg cctgaatgct gttcgcgtgg cgcggccact gttacgtaac
    10441 cgagttttgc cagtttacgg gcagcggccc acaacccggg agaatggatg ccgtcatcgt
    10501 ttgtaaggag tatctgtggt tttttcataa agcgaatcca gtgatcctgt tttctataga
    10561 aaaaagagcg gtgtgccgct cttttttagg tgcccccgga gtaattcgaa tactcaacct
    10621 tttgctccgc aagcaaacgc tctatccaat tgcgctacgg gggcatgcga tattgagcgc
    10681 ctgaaatata ccccaaccca ccagaaacgt caaggaaaac gccaact
//
LOCUS       DAOSPM010000060        10693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_6169_length_10693_cov_13.311807, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000060 DAOSPM010000000
VERSION     DAOSPM010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6169_length_10693_cov_13.311807"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLW25_06345"
     CDS             complement(<1..1136)
                     /locus_tag="PLW25_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOS54142.1"
                     /translation="MIQISTWISTPYKTSMYRPLHIDRSWVFQEPVPVPRALTSLGDY
                     PDIVFQTLTRRGFNDIHLARQFIDPAAYEPSPPVELPDMDRAVSRILHAVDSAQRIGV
                     WGDFDVDGQTSTAILISALRTLGTDPVFHIPVRGIETHGIGMAPLQTFLDQGPELILT
                     CDTGVSSFDAVKYAQSRGVDVIITDHHELPETLPPAYALVNPHRLPEGHRLSSLPGAG
                     TAFKLAEALLTERQMAEKARHLFDLAALGCVADLASLTGETRYLVQSGLNQLRTNPRP
                     ALAALLQSSSVEIMRTSEEHISFALAPRLNAVGRLSDANPIVNFLLSENPVEIAAMVN
                     QIEGLNTQRKYLSDQVFQGALTQLEQERSLLDQPILILHHQEWPA"
     gene            complement(1099..1905)
                     /locus_tag="PLW25_06350"
     CDS             complement(1099..1905)
                     /locus_tag="PLW25_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOS54143.1"
                     /translation="MKLPDHFSFSQYNLQDYVDCKFRFLLKHIHQLAWPAIESEPLLL
                     QEARMELGQQFHRLVQQYFIGVEPGVLSNSIQTSELADWWQAFLSLKINEQPGTKQAE
                     KMLSVSLNGHRLVAKFDLLLQRSSSSYTIYDWKTSTQAPSIRHLQGRLQSHVYPLVLW
                     LSVTNSVTPPQNLPEIEMVYWFPAFPEVSLRFEYTHSNYEQDLDYLTHLIDEIQEMKE
                     EDFTRTTDVKRCAYCQYRSLCDRGISAGALTPGSEVDSLDSDFYLDFDSL"
     gene            complement(1892..4129)
                     /locus_tag="PLW25_06355"
     CDS             complement(1892..4129)
                     /locus_tag="PLW25_06355"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOS54144.1"
                     /translation="MFKPRENQVRILQYQRGKMGISAVPGSGKTHTLSCLASLLILKG
                     ELQNDQEILIVTLVNSAVDNFLNRIGGFIREAQLLENIGYRVRTLHGLAHDIIQERPD
                     LAGVANQFSIIDEIESERMIAQLSAAYIGLHPEIVSHLQNPEADLSSAGRTRNDFSSL
                     VSKLNQDFISQAKDLQAEPETILNRVQRYNCSDPLIKMGIEVYQQYQRGLRYRNALDF
                     SDLIRLAYTALLNDDGYLQRLRQRWPYILEDEAQDSSNLQEKMLRLLVGPQGNWVRVG
                     DPNQAIYETFTTANPKFLKAFLKEQGVQEMTLPHSGRSNLSIITLANSLVQWTRESHP
                     LPSLRSALDYPLIEPTPPGDPQPNPPDHPSAVYISKMKATADEEIKKVVENILNWLPG
                     NQDKTVAVLTPIGWYGEKMAEALHAAGVPMVELLKSSQSTRRVAGLIEKILLALDDPN
                     SSKKFTEAIQSVWTWDTVEGDKLNEIKSLLPLLKKCSRPEDFFFPQPGKTWREAIEPF
                     EVSEERLQEFEDLRLKFTRWQKASFLPIDQLVLTIANDLFSVPSDLALAHKFALLLEF
                     STHLHPAYELKDYAQELAVISGNFRKFSGFSDEELLFDPDAHKGKVFISTYHKAKGLE
                     WDRVYLMSVNNYDFPSAQPYDTYIGEKWFIRDNFNPQAEMLSRLTGLLDGDEALIFAP
                     DGQATAAARVDYAAERLRLLFVGITRARQSLVVTWNSGKNKDKTMALPLEALHAIWRG
                     TDEAA"
     gene            complement(4122..6269)
                     /locus_tag="PLW25_06360"
     CDS             complement(4122..6269)
                     /locus_tag="PLW25_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54145.1"
                     /translation="MNPTHLVLVVNASLTEKQSAVITAPARGSLLVTGKAGTGKSTAA
                     VLRMRQMVENGIDGDSILVLVPQRRLAFEYYKAIHSPDFPAGSQPQLLTFNGLAQRML
                     SLFWPLIATDAGFRAAEPPRFLNMENAQYYLAGIVEPLLLQGYFESLTIDPNRLYSQI
                     LDNLNKSAVVGFPPGEIAERLTSAWSGKTSKSNIYQQAQECALKFRQFCLENNFLDFS
                     LQLSVFREKLWPSLLCRQHLNQTFRHLIYDNIEEDFPVAHDFVEEILPNLDSALLIQD
                     EGGGYRSFLGADATSAQRLSGKCSSQIRLTSSLVQTNALANLETALRESILEHRLLKY
                     DSSDARSSYTIHSFRFYPEALDWVTGEIVKLLKDSTTQPGDIAILTPYLSDALRFSFA
                     NRLSSANIPFTTYRPSRSLYAEPAVQAVLTLAQMAHPSWQSKPSPQQARSAFAMVIAA
                     CDFVRADLISRTLFKNRDNLFPLLSFQGLNLEMQSRISFQVGEYYERLRAWLNENLEQ
                     GAHELDHWISRLFGECLSQPGFGFHRDYDAASSINRLIESCREFRKIYQPSAKTAASG
                     AGEEFTRVLQNGLLSSQSNPRSLFETNTDVVFLSPAYSFLMRNQPVKYQFWVDIGSGG
                     WWSRLDQPLTQPYVLNRNWSINQKWTDEHEYLNNQLTLARVVTGLVRRCSAHIYMCSV
                     TYNEHGFEERGQLLLALQTVLRANASIKGSADV"
     gene            complement(6266..6841)
                     /locus_tag="PLW25_06365"
     CDS             complement(6266..6841)
                     /locus_tag="PLW25_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOS54146.1"
                     /translation="MTSLTDNQQQIRESARQILALKPVYLDTETTGIGSVDEIVEISV
                     IDHDGAQLFSKLVKPTRPIPPGAERIHGISNRQVADAQPWPILWPELRSILYGRTIAA
                     YNSSFDARLMEQTHSRYRLPWRERLNFLDVMQLFSDFRAVWDPFRKTYRYFKLAEAGA
                     FFQIDLLNAHRSSADALLTRAVLHSIAGLPY"
     gene            complement(6871..7719)
                     /locus_tag="PLW25_06370"
     CDS             complement(6871..7719)
                     /locus_tag="PLW25_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54147.1"
                     /translation="MKKLAIVLGFMGIVLTACNFPLGKLPNDQSQIATRVAATLSAAQ
                     TAAIPTPSSLETIAIVLPTEGPTLTPSPTDTLTPTVTITAPANDPALTLGTPGFFDTF
                     NNGVSFGLATPYEDEGMIIKVESGSMVFKSLALKAGKRWRLTSRNPKNLYLEGTFRTS
                     SCSGTDQYGLVFRAPTYGDGIGYYFGVSCNGQYYFLRWDNSGPTTLVNWTPESKINAG
                     SNQTNRLGVMANDDTFRFYINGSLVKEISDQGITEKGYIGAFASAFEDPNFTVLLDEI
                     SLWPLQ"
     gene            7825..9333
                     /locus_tag="PLW25_06375"
     CDS             7825..9333
                     /locus_tag="PLW25_06375"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HOS54148.1"
                     /translation="MKTVILQIEAYDTVASIRDKIKGCRSKRILLVHPRQKRAFPGRL
                     ELDLIARYARENGAAFALVSRNDITREHAESLGISVFGSIPEAQKGEWHEADNPLGQT
                     TPFRGHDELIRMRNNLPKTAATREIRPLVRYLLVGISFLALAALMVFSLPSATLVVSP
                     VPEVQTMTIDIRASTEIAQANLTGLLPAKEESLSVSGQKTIPATGSITVGVEKAHGEV
                     LVRNLFTEAQEIPEGAIFETGGEPNIRFRALDEVNLPADGKGVLVKVEAVLPGAEGNV
                     EPGMVRYLAGGNGLQVAVMNNEPITGGKSINVNSPTEADYQNARLQLLRELEEKALKE
                     IKATVSQDITAIPNSLKFTRVETEERENPIGEPSDMLTINMTATFKMLVYDTVALFSL
                     GEEVMDLSLPEGYHAAKDPLKMIQQGEAVLDQSGEASWQVTFSRQIYRNLQWQQLIPS
                     FRGREKRDVVKVLDEEIPHVKGAEVLTFPEWWPWLPLISDRIYLVERASGNG"
     gene            9338..9760
                     /gene="ruvX"
                     /locus_tag="PLW25_06380"
     CDS             9338..9760
                     /gene="ruvX"
                     /locus_tag="PLW25_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOS54149.1"
                     /translation="MAVDPGEKRLGIAISDPYGVLATPLTVIRHESMTVDCLKIIELC
                     AQNDVSLIVIGQALSASGEESPQVRHTHKLAQTIRSLSPLPVLFWDESESTNLAKKAA
                     IEMGVKKKKRAGHLDEHAAAVILQSYLEAQKGRQANEA"
     gene            9750..>10693
                     /gene="mltG"
                     /locus_tag="PLW25_06385"
     CDS             9750..>10693
                     /gene="mltG"
                     /locus_tag="PLW25_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOS54150.1"
                     /translation="MKPKRHPVFPGCVLLVLLFGIFLSILWVGIPIIARWQFGAPAEQ
                     LTGFSLRNFGLQTLLHRSDLTQASSKAGGEIDFTIESSESINSISHRLAAAGLIKDAG
                     GFRAYLVYKGLDSRIKAGAYKLDPAMTSIEIAERLQAIYSPIVPFFIYPGWRAEEIAA
                     ALPSSGIEVSPEEFLAIVHNPSSLAELTPLSEQSSLNGFLFPGAYQIERKISPDALVL
                     TFVNRFFESVNPGIVSAIHQQGISLYEGITLASIIQRETFEDEERALMASVFYNRLRD
                     GIKLETDPTVQYALGYSAKWGGWWKTPLQTKDLEVDSP"
BASE COUNT         2725 a         2495 c         2875 g         2598 t
ORIGIN      
        1 gcaggccatt cctggtggtg gagaatgagt attggttgat cgagcaaaga gcgctcttgt
       61 tccagttggg tcaaagcacc ctggaaaacc tggtcgctta gatattttcg ctgtgtattc
      121 agtccttcga tttggttaac catcgccgcg atttctaccg ggttctctga aagcaagaaa
      181 ttgacgatgg ggttggcatc gctgagcctt cctactgcgt tcaggcgtgg agccagagca
      241 aaggagatgt gttcttctga ggttctcatt atttcgacag aagaagactg gagcagcgct
      301 gccagcgctg ggcgcgggtt ggtacgcaat tgattcaaac ccgattgcac caggtaacgg
      361 gtttcaccag tgagggaagc caggtctgcc acacaaccaa gcgcggcaag gtcgaacaaa
      421 tgacgggctt tttctgccat ttgccgttcg gtcaacaagg cttcagccag tttgaaagcg
      481 gtcccggcgc ctggcaaact gcttaatcgg tgcccctctg ggaggcggtg cggattcacc
      541 aaggcgtaag ccggtgggag cgtttcaggc agttcgtgat ggtcggtgat gatgacatcg
      601 acgccgcgtg attgggcgta tttgacggcg tcaaaagatg aaacacctgt gtcgcaggtg
      661 aggatcaatt ctgggccttg gtctaagaag gtttgcagtg gagccattcc aattccgtgt
      721 gtttcaattc ctcgcaccgg aatgtggaag acaggatcag tgccaagcgt tctcaatgct
      781 gaaatcagaa tggcggttga agtttgcccg tccacatcga agtctcccca aacaccaatt
      841 ctttgagcag aatcgacagc gtgcaagata cgcgagacgg ctctatccat atccggtaat
      901 tcaacaggcg gacttggctc gtaggcggcg gggtctataa attgccgggc gagatgaatg
      961 tcattaaaac ccctgcgtgt caacgtttga aaaacaatat cagggtaatc ccccagcgaa
     1021 gtcaaggccc tgggaaccgg tactggctct tgaaataccc aactgcggtc aatgtggagt
     1081 ggtcggtaca tactggtttt atagggagtc gaaatccagg tagaaatctg aatcaagtga
     1141 atctacctct gaaccgggtg tgagcgcgcc ggcggaaatc ccccggtcac acaaagaacg
     1201 atattggcaa tacgcgcaac gctttacgtc cgttgtgcgg gtgaagtctt cctccttcat
     1261 ttcttgtatc tcgtcgatta aatgtgtcag gtaatccaaa tcctgttcat agttagaatg
     1321 cgtgtactca aaccggagcg atacctccgg gaaggctggg aaccagtaga ccatttcgat
     1381 ctcgggtaaa ttctggggtg gggtcacgct gtttgtaaca gaaagccaca gcaccagagg
     1441 gtaaacatgg ctctgcaacc gtccttggag atgtcgtatg gagggcgctt gagtgctggt
     1501 tttccagtcg taaatcgtat aagacgatga agatctctgc agcagcaggt cgaattttgc
     1561 taccaggcga tgcccattca aggaaacgga taacattttc tcagcctgct tcgtgcctgg
     1621 ctgttcattg attttgagag aaagaaaagc ctgccaccaa tctgccagtt ctgaggtttg
     1681 aatggaattg gacaggaccc ctggctcaac cccgatgaag tactgttgta ctaagcggtg
     1741 aaattgctgc ccaagctcca tacgcgcttc ctgcaataat aaaggttcgc tttcaatcgc
     1801 cggccatgcc aattggtgga tgtgctttaa caagaagcgg aatttgcagt ccacataatc
     1861 ctgcaggtta tattgagaaa atgaaaaatg gtcaggcagc ttcatcagtg cctctccaga
     1921 tggcgtgaag ggcttctagg ggaagcgcca tcgtcttgtc tttatttttg ccggagttcc
     1981 aggtgaccac cagcgattga cgggctcgag tgatgccaac aaagagcagg cgcagccgtt
     2041 cagcagcgta atccactcgg gcggctgcag tggcttgacc atcgggggca aaaatcaggg
     2101 cttcatcccc atccagtagt cctgtaaggc gagacaacat ctccgcctgt gggttgaaat
     2161 tgtcgcgaat gaaccatttt tcaccaatat aagtatcgta tggttgagca gaaggaaaat
     2221 catagttatt gaccgacatc aggtaaaccc ggtcccactc gagcccttta gctttgtgat
     2281 aagttgaaat aaacactttg cccttgtgag cgtcggggtc aaaaagcagt tcttcatcac
     2341 tgaaaccgct gaactttcga aaattaccag aaatcaccgc gagctcttgg gcataatctt
     2401 ttagttcgta ggctgggtga aggtgagtgc tgaactccaa tagcaaagca aacttgtgcg
     2461 ccagcgccaa atcagaaggc acagagaaaa gatcgttagc gatagtaagc accagttggt
     2521 caattggaag gaaagatgcc ttttgccaac gggtgaattt caagcgcaaa tcctcaaatt
     2581 cttgcagccg ctcttccgaa acctcaaaag gctcaatggc ttctctccag gttttaccag
     2641 gttgagggaa gaagaaatcc tccggtcggg agcatttctt gagcaatggt agtagcgact
     2701 tgatttcatt aagtttatcg ccctctacag tatcccaggt ccatacagac tggattgcct
     2761 cagtgaattt cttgctgctg ttggggtcat ccagcgcaag cagaattttt tcaatcaacc
     2821 cggcgacacg gcgtgttgac tggctgcttt tcagcaattc caccatcggc acaccggcag
     2881 cgtgcagcgc ttctgccatt ttttcaccat accagccgat aggtgtgagc acggccactg
     2941 tcttatcctg gttgccggga agccaattca ggatgttttc gactactttt ttaatttctt
     3001 catccgcagt ggctttcatt ttactgatgt aaaccgcgct tggatgatcg ggaggatttg
     3061 gttgagggtc tccgggagga gtgggttcga tgagcgggta atcgagcgcg ctgcgcagcg
     3121 agggaagcgg gtgagattcc ctggtccact ggacgagtga gttggccagc gtaataatgc
     3181 tgaggtttga tctgcccgag tgaggcaggg tcatttcttg cacgccttgc tctttgagaa
     3241 aggctttaag aaatttcgga ttggcggtag taaacgtttc gtaaatagcc tggttggggt
     3301 cgcccacacg cacccagttc ccctgaggac caacgagcag gcgcaacatt ttttcctgca
     3361 agttagaaga atcctgcgct tcatcttcca gaatatatgg ccagcgttga cgcaggcgct
     3421 gaagatatcc atcgtcattg agtagggcag tatacgccag gcggatgagg tctgagaagt
     3481 cgagtgcgtt gcggtaacgc aacccgcgtt gatattgttg gtacacctcg atccccatct
     3541 taatcagcgg gtcggaacaa ttgtagcgtt gcacgcggtt gagaatggtc tcaggttcgg
     3601 cctgcaggtc tttcgcctgg ctgatgaaat cctggttcag ttttgaaacc agtgaggaaa
     3661 aatcgttccg cgtacgcccg gctgaagaaa ggtcggcctc tgggttttgc agatgagaaa
     3721 cgatttcagg atgtaaacca atgtacgcag ctgataactg cgcaatcata cgttctgatt
     3781 caatctcatc gatgattgaa aactggttgg ccacaccagc aaggtcaggt ctttcctgga
     3841 tgatatcgtg agccaaaccg tgcagtgtcc tcacacggta accgatgttt tccaataact
     3901 gcgcttctcg aataaaacca ccaatgcggt tgaggaaatt atcaactgcg gaattgacca
     3961 gcgtcacgat gaggatttcc tgatcgtttt gcagctcccc cttgagaatc agcagggaag
     4021 ccaggcagga aagggtgtgc gtcttgccgc tgccgggaac agcagaaatc cccatttttc
     4081 cgcgttggta ctgcaaaata cgaacctggt tctcgcgtgg tttaaacatc tgcactacct
     4141 tttatactcg cgtttgcacg cagaacagtt tgtagtgcta acagcaattg accgcgttct
     4201 tcgaagccat gctcattata ggtgacgctg cacatataga tgtgagcact acagcgcctg
     4261 accaaaccgg tgacaacccg agcaagcgtc aattgattat tgagatactc gtgctcatca
     4321 gtccattttt gattgataga ccagttacga ttgagcacat agggttgggt taagggctgg
     4381 tctaacctgg accaccaacc gcctgaacca atatcgaccc agaactgata tttaaccggc
     4441 tgattgcgca ttaaaaaaga gtaagcggga gaaagaaaaa ctacgtccgt attggtttca
     4501 aaaagagaac gcgggttgga ctgagaggag agcaagccat tctgcaacac acgggtgaat
     4561 tcttcaccag cgcctgaagc cgcagtcttc gcgctcggct gataaatttt acgaaattcc
     4621 cgacaggatt cgatcagacg gttaatcgac gacgcggcat cataatctcg atgaaagccg
     4681 aagccgggct gggaaagaca ttcgccaaaa agccggctga tccaatggtc gagctcatgt
     4741 gcgccctgtt ccagattctc gttcagccaa gcgcgcaggc gttcgtaata ctcgcccacc
     4801 tggaaggaaa ttcgagattg catctcgagg ttgagcccct ggaaagaaag aagaggaaag
     4861 aggttgtccc tgtttttaaa aagcgtacgg gaaatgagat ctgcccgtac aaaatcacac
     4921 gcggcaatga ccatggcaaa cgctgatctg gcctgctgtg gggagggttt gctctgccat
     4981 gaaggatgcg ccatttgggc gagggtaagg accgcctgca ccgcgggctc tgcatataaa
     5041 ctgcgcgaag gacggtaggt cgtgaaaggg atgttcgcgc tcgacaaacg attggcgaat
     5101 gaaaaacgca aggcatcact gaggtacgga gtgaggatcg cgatgtcgcc aggctgtgtg
     5161 gtactgtctt ttagcagttt aacaatttca cctgtgaccc aatcgagcgc ctcggggtaa
     5221 aaacgaaagg aatggatggt atagctgcta cgcgcgtccg aggagtcgta cttcaacaat
     5281 cgatgttcaa ggatgctttc acgtaacgcc gtttcaaggt ttgccaacgc attggtttgc
     5341 accagcgatg aggtcaggcg gatctgactg gaacatttgc ccgataggcg ctgggcgctg
     5401 gtggcatccg cgccaagaaa tgagcgataa cccccgcctt catcttgtat caacagcgcg
     5461 gaatcaaggt tgggaaggat ctcctctaca aaatcgtggg caacagggaa atcttcttct
     5521 atgttatcgt agataaggtg acggaaggtc tggttcaggt gttgcctgca gagcagcgag
     5581 ggccacagtt tttcgcggaa aacggataat tgcagtgaaa agtcgaggaa gttgttctcc
     5641 aggcaaaact gccggaactt taaggcacat tcctgagcct gttgatagat attggatttg
     5701 gaggttttac cagaccaggc agatgtaagc cgctcagcga tttcaccagg agggaatcct
     5761 actacggcag atttgttcag gttgtcaagt atctggctgt aaagtcggtt cgggtcaatt
     5821 gtcaggcttt caaagtatcc ctgcagcaac aacggctcaa caatacctgc caggtaatac
     5881 tgggcatttt ccatgttgag aaaccgtgga ggttctgccg cgcgaaaacc agcatctgtg
     5941 gcgataagcg gccagaataa ggaaagcatg cgttgagcca gcccattgaa agtaagtaac
     6001 tgtggctgac tgccggctgg gaaatctggt gaatggattg ccttgtaata ctcaaatgcc
     6061 agacggcgct gcggcacaag gacaaggatg ctgtcgccat cgataccgtt ttccaccatc
     6121 tggcgcatgc gcagcactgc cgcggtagat ttgcctgtac cggctttccc cgtcacaagg
     6181 aggctgccac gtgctggggc agtaatcacc gcggattgtt tttctgtgag tgaagcattt
     6241 acaacaagta cgagatgtgt tggattcaat acggcaaccc ggcaatcgag tgaagtacag
     6301 ccctggtgag cagggcgtca gcggaagagc ggtgggcgtt gagtaagtca atctgaaaaa
     6361 atgcgcctgc ttcggcaagt ttaaagtaac ggtaggtttt tcggaaaggg tcccacacag
     6421 cgcggaaatc agagaacaat tgcatcacat ccaggaaatt cagcctctcg cgccagggca
     6481 ggcggtaacg cgagtgagtt tgttccatta gcctggcatc aaaggacgaa ttgtaagccg
     6541 cgatggttct tccatataga atcgacctga gctccggcca aaggataggc cagggttggg
     6601 cgtcagccac ttgacgattg gaaatcccat gtattctttc cgctcctggc ggaatgggcc
     6661 tggtaggttt caccagtttt gagaaaagtt gagcgccgtc atggtcgatc acggatatct
     6721 ccacaatttc atccacgctg cctatgccgg ttgtttcagt atccaggtag acgggtttga
     6781 gcgccagaat ttggcgcgca gattcacgta tttgttgttg attgtctgtc aacgatgtca
     6841 atacgcaggt ccttatttga gctttttaga ttattgtaga ggccaaagag agatctcatc
     6901 taacaacacg gtgaagttgg ggtcctcaaa cgcggatgcg aaagcgccaa tatagccttt
     6961 ttcagtgatt ccctgatcag aaatttcttt caccagcgaa ccattgatgt aaaagcggaa
     7021 tgtatcatca ttcgccatca ctcctaaacg gttcgtttgg ttgctgcccg cgttgatctt
     7081 ggattccggt gtccagttca caagagtagt ggggcctgaa ttatcccagc gcaagaaata
     7141 atactgcccg ttgcaagaca cgccaaaata ataaccgata ccatcaccgt aagtgggcgc
     7201 acgaaacacc agcccgtatt ggtcggttcc actacaggag gaagtgcgga aggtgccttc
     7261 caggtacagg tttttgggat ttctggacgt caaccgccac ctttttcccg ctttgagggc
     7321 aaggctttta aagaccatgg agccgctttc tacttttatt atcatgccct cgtcctcgta
     7381 gggggtcgcc aggccaaaag aaactccgtt attaaaggtg tcaaaaaagc caggagtgcc
     7441 aagggtaagg gctggatcat ttgcgggggc ggtgatagtg actgtgggcg ttaacgtatc
     7501 ggtgggcgat ggggtcaggg taggcccttc tgtcggtagc acgattgcga tggtctcgag
     7561 cgaggaaggg gtgggaatag cggcggtttg cgccgcggac aatgtcgctg cgactctcgt
     7621 ggcgatctga ctttggtcgt tgggaagttt acccagaggg aaattacagg cggtaagcac
     7681 gatacccatg aaaccaagta cgatggcaag ttttttcatc tttacctcac ttttcttttt
     7741 ctattataaa ctctgtcaat gtgaatagaa cagtgagggg cggattaatt tcaaccagcc
     7801 ctacagtata attctgtaaa ccctatgaaa acggtgattt tgcaaattga agcctacgat
     7861 actgtcgctt cgatccgcga taaaatcaaa ggctgccggt caaaaagaat cttactggtt
     7921 catcctcgtc aaaaacgggc attcccaggc aggctcgaac tggacctgat tgctcgctat
     7981 gcgcgagaaa acggcgccgc ctttgcgctt gtcagccgta acgatattac gcgtgaacac
     8041 gccgagagtt taggcatttc tgtttttggt tctatcccag aggcgcagaa gggagaatgg
     8101 cacgaagccg acaacccgct gggtcagaca actccattcc gtgggcatga tgaactaatt
     8161 agaatgagga ataatctgcc aaaaaccgct gcaacacgcg aaattcgccc tcttgttcgc
     8221 tacctgctgg ttgggatttc tttccttgct ttggcggcat tgatggtctt ttcgcttccg
     8281 tctgctactt tggtagtatc ccctgtacca gaagtgcaaa caatgacaat tgatattcgt
     8341 gcatctacag aaatcgccca agcaaacctt accggtcttt tacctgcaaa agaagagagt
     8401 ttgtctgtat cggggcagaa aacgattcct gctacaggca gcatcacggt gggcgttgaa
     8461 aaagcccacg gagaagtttt ggtgcgcaat ttgttcacag aagcgcaaga aatacctgaa
     8521 ggcgcgatct ttgaaactgg cggagaaccg aatatacgat ttcgcgcact tgatgaagtt
     8581 aacctgcccg cagatggtaa gggggttctc gtgaaagtag aagcggtatt gcctggggca
     8641 gagggaaacg tagaacccgg tatggtcaga tatctggcag gtggtaatgg cttgcaggtg
     8701 gctgtaatga acaatgaacc gataacagga gggaaatcaa tcaacgttaa ttcgccaacc
     8761 gaagcggact atcaaaacgc gcgacttcaa ttgctgcgtg aactggaaga aaaggcattg
     8821 aaggagatca aggccaccgt ttctcaagac atcaccgcaa taccaaacag cctgaaattc
     8881 acccgcgtgg aaactgaaga gcgagaaaac ccgattggtg aaccttcaga catgctcacg
     8941 ataaacatga cggcgacttt taaaatgctt gtttacgaca ctgttgcact cttttcactg
     9001 ggtgaagaag taatggatct ttcactgcct gagggttacc atgctgcgaa agacccactt
     9061 aaaatgattc agcaaggcga agctgttctc gaccagtcgg gagaagcttc ctggcaagtc
     9121 actttttcac gacagatcta tagaaacctt caatggcaac aacttattcc ctcttttaga
     9181 gggcgagaga agagagacgt cgtaaaggta ctcgatgaag aaatacctca tgtaaaagga
     9241 gcggaagttc tgactttccc tgaatggtgg ccctggctgc ctttgatttc agatcgaata
     9301 tacctggttg aaagggcaag cggcaatggg tagggttttg gcagtagacc ctggtgaaaa
     9361 gcgcttggga attgccatca gcgaccctta tggcgttctg gcaacgcccc tgaccgtgat
     9421 cagacatgaa agcatgaccg ttgattgcct taagatcatc gagctatgcg cgcaaaatga
     9481 tgtcagcctg atcgtaatcg ggcaggcgct ttcagcaagc ggtgaagaga gcccgcaagt
     9541 caggcacaca cacaaattag cgcaaacgat ccgctctttg tctcctttac cggttctctt
     9601 ttgggatgaa agcgaaagca ccaacctggc taaaaaagca gctatcgaaa tgggagtgaa
     9661 gaagaaaaag cgcgccggtc acctggatga gcacgccgcc gcggtaattt tacaatcgta
     9721 cctcgaggcg cagaaaggca ggcaggcgaa tgaagcctaa gcgacatccg gtttttccag
     9781 gttgcgtatt actggtattg ctcttcggta tatttttgtc cattttatgg gtgggtatcc
     9841 cgataatagc caggtggcaa ttcggggcgc cggcagaaca actgaccggt ttttcgctca
     9901 ggaacttcgg gctgcaaacc ctgcttcatc gaagtgatct gactcaagcc tcctcaaagg
     9961 caggcggaga gatcgacttt acgattgaaa gctcagaatc cattaatagc atcagccacc
    10021 ggttggccgc agcggggctg attaaagacg caggcgggtt tagagcttac ctggtttaca
    10081 aagggttgga ttcacgcatt aaagccggcg cttataaatt ggatccagcg atgaccagta
    10141 tcgaaattgc cgagaggtta caggctattt attcgccaat cgtccccttc tttatttacc
    10201 ctggctggcg ggctgaagaa atcgcagcag cgttgccatc ttcgggtatt gaagtgtcgc
    10261 cggaggaatt tcttgccatt gttcacaacc catcttctct tgcagaatta accccgctga
    10321 gcgaacagag ttctttgaat ggctttctct ttcctggagc gtatcagatt gagcgaaaga
    10381 tcagcccgga tgcgcttgta ctaacatttg tcaatcggtt ttttgaaagc gtgaaccctg
    10441 gtattgtgag cgctattcac caacagggaa taagtcttta tgaaggaatc acactggcgt
    10501 ccatcatcca acgggagacc tttgaagatg aggaacgcgc actgatggca tccgtttttt
    10561 ataatcggtt gcgcgacggg attaaactcg agactgatcc taccgtgcag tacgcgttgg
    10621 gatattcagc caaatggggc gggtggtgga aaacaccttt gcaaacgaaa gacctggagg
    10681 ttgattcgcc tta
//
LOCUS       DAOSPM010000061        10674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_6189_length_10674_cov_11.879085, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000061 DAOSPM010000000
VERSION     DAOSPM010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10674
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6189_length_10674_cov_11.879085"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLW25_06390"
     CDS             <1..209
                     /locus_tag="PLW25_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54151.1"
                     /translation="EGLTNGLRSIRWASEKSFGFEALNTAFVEVLNNTAETLRGTQTG
                     ILNWNVLAISAGLIILFVVVVVGG"
     gene            211..1722
                     /locus_tag="PLW25_06395"
     CDS             211..1722
                     /locus_tag="PLW25_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOS54152.1"
                     /translation="MNKVLLLKSLLILPFVAAVIVYLVGRISLRKEKDWGFSVARALT
                     VFVLLVEGCLLFFGVRMALQSGGLYLDVGEITLMLDGIGLLLIAVVLFLGLMVALFSV
                     DYMAGEKGEEKYYALLLATIGSVIGLGLSFDLFNLWVWFEAMAVSTFLLVAFYNDQPP
                     SLEAGIKYLAQSAVGSALVLFGIAFIFSQTGSTDFVDIWQGATQNPKVMLISGILLIV
                     GFGVKAALVPLHTWLPDAHSQSPSGISAMLSGVVIEAGLLAMLRSLSFFGRVEFNWGA
                     TLLVFGCLNILIGNLLALRQTEIKRLLAYSSLAHVGYMLVGFGMGFWYGQANGAGGGF
                     FHLITHSLMKGLAFLAAGALLYALHLQNDEHVPLTLSDLNGASRKYPLAAFALSVALL
                     ALGGLPPLAGFMSKWQILAAVASAKNTLMILVVIFVGLNSVLSLGYYAPIVNRLYRRE
                     TSPAVENGKPIPVMMVIPLVILVLALVVIGLWPASMDFFTEKATSSLLFPFFY"
     gene            1737..2048
                     /locus_tag="PLW25_06400"
     CDS             1737..2048
                     /locus_tag="PLW25_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54153.1"
                     /translation="MQILTSTPIALLIYLALVTLLYLVGKALAGKENPTPEKSSLYGS
                     GEEAPTSFASPGYRPFFLIAFFFAILHLGVLVIGSGEFSFKLIPILLGLILALVALIL
                     G"
     gene            2071..2517
                     /locus_tag="PLW25_06405"
     CDS             2071..2517
                     /locus_tag="PLW25_06405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOS54154.1"
                     /translation="MNNPIQNVMEKIKTWARTNSPWAIHFNSGSCNGCDIEILATLTP
                     RYDVERFGIKLQGSPRHADVLICTGPVTLQARDRLVRIYEQMPEPKFVVAVGSCSISG
                     GVFDGCYNVVGDISEVIPVNVYVPGCPPRPEAIIYGIEQLLKTLQP"
     gene            2532..3041
                     /locus_tag="PLW25_06410"
     CDS             2532..3041
                     /locus_tag="PLW25_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOS54155.1"
                     /translation="MNTQEALEKAEAILKDLALSFTCPEENRLDAVIKADDLKKAVEA
                     LLVTNRWGYLSALTGLDNPEYEVDEDTREKVAEEGKGSLEVLYHFAAGAAITTLRVSL
                     PYDAAEIDSICDLVPAATLYEREAMELFGICFKGTPSTEHLVLPDCWPNGVYPLRKTF
                     KGLNEKAQG"
     gene            3054..4235
                     /locus_tag="PLW25_06415"
     CDS             3054..4235
                     /locus_tag="PLW25_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOS54156.1"
                     /translation="MPRQKSERFIVPIGPQHPALKEPGHFEFTVEGEVVTNATARLGF
                     VHRGMEKGAESRTWTQDLYLMERVCGICSHVHSMAFVLGVEKLANVQAPPRAQAIREL
                     VAELERIHSHILWLGVAAHEGGFDTLFMFSWRDRETVMDLLETISGNRVNYSANVLGG
                     VKVDINERQKDELLRGMDFLEERTRHYLKVVTEDDTFLGRTRNIGMMTFEEATRLGAI
                     GPTGRASGLLRDVRMDSPYAGYPDFPIQIVTAKAGDLEARFVVRIKELFESYRLVREI
                     VEKIPSGDLTTKIPRRIPEGETVSRVEAPRGELFYYIKSNGTDRPERVKVRTPSLCNW
                     ASVLTTAIGHKLADMPMILAGIDPCFSCNDRMVVLNGPAGQSLWNWEKLRQYGIEYYK
                     K"
     gene            4232..5185
                     /locus_tag="PLW25_06420"
     CDS             4232..5185
                     /locus_tag="PLW25_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOS54157.1"
                     /translation="MNVTFPSLIALLLFPGGLFLLASGLVFEYVDRKFIARYQNRIGP
                     RWFQPLADLVKLLAKEEVIPAGTIKGLFIALPIAALAAALTAGLSVPLFGFRPVFSYP
                     GDLIGTLYLLSLMTLCIGLAGANTENRFSIVGATRALTQLFSYEAPFLLALLGPAIAL
                     GTWNIGEINQAAGMNLWFILSQPIGFLAALIGLMGKLELPPFDAPEAETEIVAGALTE
                     YSGRGFALFRIGRDVELVIGLTLIASFYLGGFSSPLDFLWKTAVLLLITAALQSLFAR
                     LRIDQTVGLWWRVGAILLLVQMLVIIVGSSLAPFVSRLFGG"
     gene            5187..5615
                     /locus_tag="PLW25_06425"
     CDS             5187..5615
                     /locus_tag="PLW25_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS54158.1"
                     /translation="MPIGSMLGDIVKSFFKKPVTEKYPFVKTAAPENLRGKLVWNPEN
                     CSGCQLCIKDCPANAIELITIDKVNKRFVMRYNLDKCTYCAQCVVSCRFHCLEMSDDQ
                     WELAATHKEPFEVYYGREEDIKFLLEKAALGGPEEACPEA"
     gene            5638..6111
                     /locus_tag="PLW25_06430"
     CDS             5638..6111
                     /locus_tag="PLW25_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HOS54159.1"
                     /translation="MGAELNGDDAVGLRVADRLASLVGQQENLLVLNGGSLPESTSGA
                     LRRFHPTLVLFVDAADLGKTAGAIEVLETQRVGGASFSTHGMPLIMLMDYLAEELGCE
                     SVLIGVQPEWVDFDSPLSDRAKKAANRLVKELYHLLAVPNLDEEELLAKKEDLGV"
     gene            6111..6821
                     /locus_tag="PLW25_06435"
     CDS             6111..6821
                     /locus_tag="PLW25_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS54160.1"
                     /translation="MAEYNQRHSKATTILALIPAYNEAERITAVITGTRALLPVLVVD
                     DGSTDDTAAISSALGAEVLRQLPNQGKGAALLAGFRYALEMGYQAVVTLDADGQHDPQ
                     EIPLFIHAFEEGADLVIGMRDFKKMPFPRNISNTIGTWLFSWALGQYCPDNQSGYRLH
                     SRRMIETSLQSAETNFEFEVEIITRCVEQGYLLRWVPIKTIYAGEKSHIKPLRHIRHF
                     FRLVRGTRHRLQQLKKQP"
     gene            7054..7509
                     /locus_tag="PLW25_06440"
     CDS             7054..7509
                     /locus_tag="PLW25_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOS54161.1"
                     /translation="MQNTRESIEKFLQLKKISVVGVSRNKDKFGNMLYRELKNSGYQV
                     MAVNPNADMVEGDRCYAGLGALPVKPEGVVVVVTPAEALQVVEEAAGLGITHIWLQPG
                     AGSRETDQKCEELNLNCISGECLFMYLEPVKSVHAFHRFIRRLLGRMPK"
     gene            complement(7545..10244)
                     /locus_tag="PLW25_06445"
     CDS             complement(7545..10244)
                     /locus_tag="PLW25_06445"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOS54162.1"
                     /translation="MATAGLAKTYDFRATEQRLYRLWEEKGYFEPSNDPRKADFDASI
                     KPFVISIPPPNVTGELHAGHAMFVSMEDLMIRYHRMKGIPTLWVPGTDHAGIATQMLV
                     EKALAKEGKKRDGMTREEFLKHTWEWKDLYHQRITSQIRRLGASCDWTRERFTLDEGL
                     SSAVREAFVRLYEKGLIYRGPRMINWSPALRTAVSDLEVEYSEEPGFLYYFKYMLADG
                     SGAYLPVATTRPETILGDTAVAVHPEDERFQRFIGKKVIVPLLNREIPVIADEYVTME
                     FGTGALKITPAHDPNDYTIGLNHGLEMINVLDTSARMNENAGPYQGMDRFECRKKIWE
                     DMRAAGLVIKEQPYTMNVPKSQRSGEIVEPMISTQWFIAIKPLAEKALEAVRDGRTRI
                     IPERFTKIYYNWMENIRDWCISRQLWWGHRIPVWYCDDCGEIIVSRQDPSACPKCGNQ
                     NIRQDEDVLDTWFSSGLWPFSTLGWPEETADLQYFYPTSVMETGADILFFWVARMMMF
                     GLEFTGQVPFHTVYLHGLILDKDGQKMSKTKGNVIDPLEIMDDYGTDALRFTLLVGST
                     PGHDTNLDIKKVEANRNFANKVWNAGRFVLSAVEKAPAEPGSEPDWTLADSWIWGRLK
                     ELLRETDRLFQSYQYGEAGRQIYEFFWSDFADWYLEIAKIQMSQGGDAAYYTATTLVR
                     VLDACLRLLHPFTPYVTEELWGHLKGAALDKAYKPFNSRRTWEDALIIAAWPEAGKSE
                     GWEEQAISSFTVIQEVVRAIRNIRADKKITPGRKLPALISAGAKMDILRGQCATIAAL
                     AGLDIEHSHISRKITEDTTGFVSVVVSGVEIFLDLAEKTDSVQDVERLRKELEVVSAQ
                     VDRLEKLLASDFATKAPPAVVEKERAKLREFIETRDKLKAQIA"
BASE COUNT         2324 a         2719 c         2975 g         2656 t
ORIGIN      
        1 tggagggatt gaccaacggt ttacgcagca tccgctgggc ttcagaaaag agttttggtt
       61 ttgaagccct gaacaccgct tttgtcgagg ttttaaataa caccgccgaa acgctgcgcg
      121 gcactcaaac cggcattttg aactggaatg tgctggcgat ttctgccggg ctgatcatct
      181 tgtttgtggt cgttgtggtg ggaggttgag atgaataaag ttcttctctt aaaaagcctc
      241 ctcattctgc cgttcgtggc tgccgtgatc gtctacctgg ttggcagaat ctcgctacgc
      301 aaggagaaag actggggatt cagcgttgcc cgagcgttaa ccgtgtttgt cttactggtt
      361 gaaggctgcc tgttgttctt cggggtaaga atggcgcttc aatctggagg gctctacctg
      421 gatgttggag agatcaccct gatgctggat ggcattggcc tcttgctgat tgctgtagtg
      481 cttttcctgg gtttaatggt cgcgttgttt tcggtggatt acatggcggg tgagaaaggg
      541 gaggagaagt attatgccct gcttttagcc accataggct ccgtgattgg gttggggctg
      601 tcatttgact tgttcaacct gtgggtctgg tttgaagcca tggcggtcag cacttttctg
      661 ctggtggctt tctacaacga tcagccgccg tcattggaag ccgggatcaa gtacctggcg
      721 caatcagcgg tgggttcggc gctggtgctg tttggtatcg cgttcatttt ctcccagacc
      781 gggagcacgg attttgtaga tatctggcaa ggggcaaccc agaaccccaa ggtgatgctt
      841 atcagcggaa ttttgttgat cgttggcttt ggcgtgaagg cggcgctcgt tccgctgcac
      901 acctggctgc cagatgccca ttcgcagtcg ccttcaggta tcagcgcgat gctttcaggg
      961 gttgtcattg aagcgggctt gctggcgatg ctgcgttcat tgagcttttt cggcagggtc
     1021 gaatttaact ggggggcaac gctgctagtg tttggttgtc tgaatatctt gattggcaac
     1081 ctgctggcac tgcggcagac agagattaaa cgcctgctgg cttattccag cctggcgcat
     1141 gtgggttata tgctggtggg ttttgggatg ggcttctggt atggacaggc aaatggcgcc
     1201 gggggagggt ttttccacct gatcactcat tccctgatga aaggcctggc ttttctggct
     1261 gccggcgcgc tgctctacgc acttcacctg cagaacgatg agcatgtccc tttgacccta
     1321 tctgacctta atggggcttc gcgtaaatac ccgttggcag cctttgcttt aagcgtggca
     1381 ctgctggcgt tgggcgggct tcctccactg gcaggtttca tgtcaaagtg gcaaattctg
     1441 gccgcggtcg cttccgcgaa aaacacgctg atgatacttg tggtcatttt tgtggggctg
     1501 aacagtgtgc tctccctggg gtattatgcg cccattgtta accgcctcta ccgtcgcgaa
     1561 acatcgcctg cggtagagaa tggcaaaccc ataccggtga tgatggtcat tccgctggtg
     1621 atattggtgc tggctttggt cgtgatcggt ctgtggcccg cgagtatgga tttcttcacc
     1681 gaaaaggcga cctccagcct gctgttccca ttcttttatt aggagaccgc atacatatgc
     1741 aaatccttac ctccactccc atcgcgctcc ttatttacct ggcgttagta acgttgctct
     1801 acctggtggg caaagccctc gccggtaagg agaacccgac gcctgagaaa tccagcctct
     1861 atggcagcgg tgaagaagcc cctacctcgt ttgcctctcc gggttaccgc ccgttttttc
     1921 tcattgcatt tttcttcgcc atactgcacc tgggtgtgct ggtgatcggc agcggagaat
     1981 tttcattcaa actcattccc atcctcctgg ggttaatcct cgcgcttgtg gctttgattc
     2041 tgggttgatg gaccattact ggagaacagc atgaacaacc ctatccaaaa tgtgatggaa
     2101 aaaataaaaa cctgggcaag gacaaactca ccttgggcga tccattttaa cagcggctcc
     2161 tgcaatggtt gtgatattga aattctcgcc acacttacac cgcgttatga tgtagagcgc
     2221 ttcggcatca agctgcaagg aagcccgcgc catgcggacg tgctcatctg tacaggcccg
     2281 gtgaccttgc aggcgcgtga caggctggtt cgcatttatg aacaaatgcc ggagcccaag
     2341 tttgtggtag ccgtcggctc ctgcagtatt tccggtggtg tatttgatgg ctgttacaat
     2401 gtggtcggcg atatcagcga ggtcatccct gtcaacgtat atgtgccagg ctgcccgccg
     2461 cggcccgagg ccatcattta cggaatcgag cagttgttga aaaccctgca gccgtaaaag
     2521 tggaggttag tttgaatact caagaagcat tggaaaaggc tgaagccatt ttaaaagacc
     2581 tggcactgtc gtttacctgc ccggaagaaa accgccttga cgcggttatc aaggcagatg
     2641 atctcaagaa ggcggtggaa gcgctgttgg tgacgaatcg atgggggtat ctctcggcgc
     2701 tgacggggct ggacaaccct gaatacgaag tcgatgaaga cacccgggag aaagtggctg
     2761 aggaagggaa gggttcactg gaggtgctgt atcattttgc ggctggcgct gcgataacca
     2821 ctctaagggt gtcgctgcct tacgacgcag cggaaataga ctctatctgt gacctggttc
     2881 ccgccgccac gctttatgaa cgggaagcca tggaattgtt cggtatctgt tttaagggca
     2941 ccccaagcac ggaacacctg gtgctgccgg attgttggcc gaatggcgtg tacccgttgc
     3001 gaaagacttt taaaggtttg aacgaaaaag cgcagggata aggaggaatg agcatgccaa
     3061 gacagaaaag cgagaggttt atcgtcccaa ttggcccgca gcacccggcc ttaaaagaac
     3121 ccggtcattt tgaatttacc gtggaaggag aagttgtcac caacgccact gccaggctgg
     3181 gctttgttca ccgcggcatg gaaaaaggcg cggagagcag aacctggacg caagaccttt
     3241 acctgatgga acgggtatgc ggcatttgtt cgcatgtaca ctccatggcg ttcgtgctgg
     3301 gggttgaaaa attggccaat gtgcaggctc ccccgcgcgc gcaggcgatc agagaactgg
     3361 tggcagagct ggagcgcatt cacagccaca tcctctggtt gggagtagcg gcgcatgaag
     3421 gcggttttga cacgctcttt atgttctcct ggcgcgaccg tgagacggtg atggatctgc
     3481 tcgaaaccat cagcgggaac cgggtgaatt attccgccaa tgtgcttggc ggcgtcaaag
     3541 tggatatcaa cgagcgccag aaagacgagc tgctgcgcgg gatggacttt ctcgaagaac
     3601 gcacgcggca ttatttgaaa gtggtaacag aagatgatac cttccttggg cgcacgcgca
     3661 atatcggcat gatgaccttt gaagaagcaa cccgattagg ggctatcggc cctactggca
     3721 gagcctcggg gctgttgcgc gatgtccgta tggattctcc ttatgctggc taccctgatt
     3781 ttcccattca aatcgtcacc gccaaggctg gagatcttga ggcacgcttt gtcgtgcgca
     3841 tcaaagagct gtttgagagc taccgcctgg tgcgcgaaat tgtggagaag attccttctg
     3901 gtgacctgac caccaaaatc ccacggcgca tccccgaagg agaaaccgtg agccgtgtag
     3961 aagcgccgcg tggagaactc ttttactata tcaaaagcaa tggcacagac aggcctgaac
     4021 gcgtgaaagt gcgcacgccc agcctgtgta attgggccag cgtgttgacc accgctattg
     4081 gtcacaaact ggctgatatg cccatgatcc tggcgggtat cgacccctgt ttttcgtgca
     4141 atgaccgtat ggtcgtattg aacgggcccg ctggtcaatc gctgtggaat tgggaaaaac
     4201 tacgccaata cggaatcgag tactataaaa aatgaatgta acctttccat cactcatcgc
     4261 cttgttgctg ttccccggag gattattcct gctcgcgagc ggcctggttt ttgaatatgt
     4321 tgaccgcaag ttcatcgcgc gttaccagaa ccgcattggt cctcgctggt tccagccgct
     4381 ggctgacctg gtcaaattac tggcgaagga ggaagtgatt ccggcgggaa caatcaaggg
     4441 attgttcatc gctttgccca tcgccgccct ggcagctgcg ctgacagccg ggttgagcgt
     4501 gccactattc gggtttagac cggttttcag ttaccccgga gacctgatcg gtaccctgta
     4561 cttactgagt ttgatgaccc tgtgtatcgg attggcgggc gccaacaccg aaaaccgttt
     4621 ttcgatcgtc ggcgccacgc gtgctttgac gcagttattc tcatatgaag cgcctttttt
     4681 actcgcgctg ctcggacccg caatcgcttt gggtacctgg aacattggcg agatcaacca
     4741 ggctgccggg atgaacctgt ggttcattct ctcgcagccc atcggttttt tagccgccct
     4801 gatcgggtta atgggtaagc ttgaactgcc gccttttgac gcccctgaag cggaaaccga
     4861 aattgtggca ggcgcgctga cggaatactc tggtcgcggt ttcgcgcttt tcaggattgg
     4921 ccgtgacgtg gaactggtca ttgggttgac gctgatcgcg tctttctacc tgggcggatt
     4981 cagctccccg ttagattttc tatggaaaac ggctgtgctg ctgcttatca ccgccgcgct
     5041 gcaatctctg tttgcccgtt tacgtatcga tcaaaccgtg ggtttgtggt ggcgtgttgg
     5101 cgccatttta ttgttggtgc agatgttggt gatcattgta ggctccagcc tggcgccttt
     5161 tgtttcccgg ctgtttggag gttagtatgc cgattggttc gatgttaggt gatattgtta
     5221 aatcgttttt caaaaaaccg gttactgaga agtacccgtt cgtgaaaacc gctgcgccag
     5281 aaaacctgcg cggcaaactg gtctggaatc cagaaaactg ctcgggctgc cagttatgca
     5341 tcaaagattg ccctgcaaac gccatcgaac tgattacgat tgacaaggtc aataaacgct
     5401 ttgtgatgcg ctacaacctg gacaaatgca cttactgcgc tcagtgtgtg gtgagctgcc
     5461 ggtttcattg tttggaaatg tcagatgacc agtgggagct ggctgcgacc cacaaggagc
     5521 cgttcgaggt ctattatggc agagaagaag acatcaagtt ccttctggaa aaagcggctc
     5581 tcggtggtcc tgaagaagca tgccctgagg cctgacacca gagtcgccgt agttggtgtg
     5641 ggcgcagaac tgaacgggga tgacgctgtc ggtctaaggg tcgccgatcg gctggcgagc
     5701 ctagtgggtc agcaggagaa tttgctggta ttgaatggcg gctcgctgcc tgagagcacc
     5761 tctggagcgt tgcggcgttt tcatccgacg cttgtccttt ttgttgatgc tgccgacctg
     5821 gggaaaacag cgggcgcaat tgaagtgtta gaaacgcaac gcgttggcgg agcctctttt
     5881 tccacacacg gaatgccttt aattatgttg atggattacc tggctgaaga actgggctgc
     5941 gagtctgtgc ttatcggcgt ccagcctgaa tgggtggatt tcgactcacc tctatcagac
     6001 cgcgctaaga aagccgccaa ccgtttggta aaagaactgt accatttgct cgcagtaccg
     6061 aacttggatg aagaagaatt gttggcgaaa aaagaagacc tcggagtttg atggcagaat
     6121 acaaccagcg gcattccaaa gcgactacaa tccttgcatt gatcccggca tacaacgaag
     6181 ccgagcgaat cactgcagtc atcacgggta cacgagccct gttgcctgta ttggtggtgg
     6241 atgacggctc aactgacgac accgctgcca tctcctcagc gttgggagcg gaggtgttac
     6301 gccagcttcc caaccaggga aagggggccg cgctgctggc ggggttccgt tatgcgctcg
     6361 aaatgggcta ccaggcagtg gtcacgctgg atgcagacgg acagcatgac cctcaggaaa
     6421 taccgttgtt tatccacgcc tttgaggagg gcgcggatct ggtgataggc atgcgggatt
     6481 tcaagaaaat gcccttccca cgcaatattt ccaacacgat cggcacctgg ttgttctcct
     6541 gggcgttggg acaatattgc ccggataacc aatccggcta tcggctgcat tcccgacgca
     6601 tgatcgaaac ctctctgcaa agcgccgaga ccaactttga attcgaggtt gagattatca
     6661 cgcgctgcgt ggaacagggc tacctgcttc gctgggtgcc gatcaagacg atctacgccg
     6721 gcgagaagag ccatatcaaa ccattacgcc acatccgcca tttctttcgc ctggtacgcg
     6781 gcacacgcca ccgcctgcag cagctcaaga aacagccatg aaagaaccct ttttcacggg
     6841 tttcccaaac cacttatcta gtagatcctg cattgtgata tagtatgaat gttttctgaa
     6901 aagtccgcac aggcgcaaga ccggaaccct ttgttgcatc tgaatggctg aaggcgaacg
     6961 aacacgcgcg cgttcctggg ttgtacgatt aactttggat tctttacctg gtccatttgc
     7021 aagcatacct ttcagcgagg aaaacagtta aaaatgcaaa acacacgcga aagcatcgaa
     7081 aaattcttgc aattgaaaaa aatttccgtg gtgggggttt cacgtaataa ggataagttt
     7141 ggcaatatgc tctacagaga gctgaagaac agcggctacc aggtgatggc ggtgaacccg
     7201 aacgcagaca tggtagaagg tgaccgctgc tacgccgggt tgggtgcact tccagtaaag
     7261 cctgaaggtg tggtggtggt tgtaacgcca gctgaggctt tacaggtcgt cgaagaggcg
     7321 gccggtttgg gtatcactca tatctggctg cagccggggg cgggttcgcg cgaaacagat
     7381 cagaaatgcg aggaattgaa cctcaattgc atcagcggcg agtgcctctt tatgtatctg
     7441 gagccggtga aatcggtgca cgcttttcac cgatttatca ggcggttgtt gggcagaatg
     7501 ccaaaataac agcgctgaaa aagcgagcgg ctttcctgtc ggttctaggc gatttgcgct
     7561 ttcaatttat cgcgcgtttc gataaactct cgtaattttg cacgttcttt ttccaccaca
     7621 gcagggggag ctttggttgc aaaatcactg gcgaggagct tttccaggcg gtccacttgc
     7681 gcgctcacga cctctaactc tttccgcagc cgttcgacat cctgaacact gtctgtcttc
     7741 tccgccaggt cgaggaatat ttcgactcct gataccacca ctgaaacgaa tccggttgta
     7801 tcttccgtaa tcttgcggct gatatgagag tgctcgatgt cgagccctgc cagcgcggca
     7861 atggtggcgc actggccgcg cagtatatcc atcttggccc cggcggagat cagcgcgggc
     7921 agcttgcgcc cgggagtgat ttttttatcg gcccggatgt tgcgaatggc gcgcaccact
     7981 tcctggatta cggtgaagct ggagatggct tgctcttccc aaccttcgct ttttcccgct
     8041 tcaggccagg ctgcgatgat cagcgcgtct tcccaggtgc gcctggagtt gaagggctta
     8101 taggctttgt caagcgcggc ccctttgaga tgaccccaaa gttcttctgt gacgtagggg
     8161 gtaaatggat ggagcaggcg caagcaggca tctaatacac gcaccagggt ggtagcagtg
     8221 tagtaggcgg cgtcaccccc ttgactcatt tggattttag cgatttccag gtaccaatcc
     8281 gcgaagtcgc tccagaagaa ttcgtaaatc tgtcgtccgg cttcaccata ctgataggat
     8341 tggaataagc ggtcggtctc gcgtaacagt tcttttaacc gaccccagat ccacgagtct
     8401 gccagtgtcc aatctggctc gctgcccggc tcagccgggg ctttttccac ggccgagagg
     8461 acgaaacgcc cagcgttcca cactttgttg gcaaaattgc ggttagcttc caccttcttg
     8521 atatcgaggt tggtatcatg cccgggagtt gaacctacca gcagggtgaa acgcagcgcg
     8581 tctgtaccgt aatcgtccat gatctccagt ggatcgataa cattgccctt ggtcttgctc
     8641 atcttttgcc catctttgtc caggattagc ccgtgcaagt acacggtatg gaagggcacc
     8701 tgaccggtga actcaagccc aaacatcatc atgcgtgcca cccagaagaa gaggatatct
     8761 gcgcccgttt ccatcaccga agtcgggtaa aagtactgta aatcagcggt ttcttctggc
     8821 cagcccaggg ttgagaaggg ccacagcccg gatgaaaacc aggtgtccag cacgtcttcg
     8881 tcttgccgga tattttgatt accgcatttg gggcaggcgg aagggtcttg gcgcgacacg
     8941 ataatttcac cacaatcatc gcagtaccac actgggatac ggtgacccca ccataactgg
     9001 cggctgatgc accaatcgcg tatgttttcc atccagttgt aatagatctt ggtaaagcgt
     9061 tcagggatga tcctggtgcg tccgtccctc accgcttcaa gcgctttttc agccagcggt
     9121 ttgatggcga tgaaccattg tgttgaaatc atcggctcca caatttcacc actgcgctgc
     9181 gatttcggca cgttcatagt gtagggctgc tctttgatca ccaaccctgc tgcgcgcatg
     9241 tcttcccata tctttttccg gcattcaaag cggtccatgc cctggtaggg accggcgttt
     9301 tcattcatac gcgcggaggt atccaggacg ttgatcatct ccagcccatg gttcaaaccg
     9361 atggtgtagt cgttggggtc atgagcgggg gtgatcttca acgcgccggt gccaaactcc
     9421 atggtcacat attcatcagc gatcaccggg atctcgcgat tcagtaaagg aacgatcacc
     9481 ttcttaccga tgaatcgctg aaagcgttca tcttccgggt gaaccgccac ggcggtatct
     9541 ccgaggatgg tttctggtcg cgtggtggca accgggagat aagcgcctga accatccgcc
     9601 agcatgtact tgaaataata cagaaaacca ggttcctcgc tgtactccac ctcgaggtct
     9661 gaaacggctg tgcgcaaagc gggagaccag ttgatcatgc gcggtccgcg gtagataaga
     9721 cctttttcat aaaggcgcac gaaagcctcg cgcacagcgc ttgaaagacc ctcatccagg
     9781 gtgaaacgct cgcgcgtcca gtcgcaagaa gctcccaggc ggcggatttg ggaagtgatg
     9841 cgctggtgat aaaggtcttt ccactcccag gtgtgcttca ggaattcttc tcgggtcatt
     9901 ccatcgcgtt ttttgccttc tttggcgagg gctttctcta ccagcatctg ggtggcgata
     9961 cccgcgtggt cagtgcccgg aacccacaat gtagggatgc ctttcatcct gtggtagcgg
    10021 atcatcaggt cttccataga gacaaacatg gcgtgaccgg cgtggagctc accggtaaca
    10081 ttgggcgggg ggattgagat gacaaaaggt tttatgctgg catcgaaatc cgccttacgt
    10141 gggtcatttg agggctcaaa ataccctttt tcctcccaca ggcggtacaa cctctgttcg
    10201 gttgcgcgga aatcgtacgt ttttgccaat cctgctgttg ccattatgtc actcctcaat
    10261 ccagaataaa aaaacctttc atccgtagag gacgaaaggc tcaatgcttc gcggtaccac
    10321 ctcaattcgc caatggtctg gcgcactctc cggattatct ccggattaag cgctaacggg
    10381 ctcacccgtg ccgttctacg ccctgacggg tttcttcggc atgtattcca aacgacttcg
    10441 gtttgtgaaa attgcggagc atttcagcct ggagctcctt ctctaccaac cttcgctcaa
    10501 acctactcct tttggcacgg ctcgtataga attatgaaag gattatacaa acataacagt
    10561 cagtcgtcaa taagagaaac gcgatgccta aaaaagttgt acaacacagc aagccctcgt
    10621 ggcttacctg ggcgcagcgc atccaggcgc ttgcgcagag cggactatac taca
//
LOCUS       DAOSPM010000062        10529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_6311_length_10529_cov_13.268188, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000062 DAOSPM010000000
VERSION     DAOSPM010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6311_length_10529_cov_13.268188"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..743)
                     /locus_tag="PLW25_06450"
     CDS             complement(3..743)
                     /locus_tag="PLW25_06450"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOS54163.1"
                     /translation="MKLNGFALKLIALVLMFGEHFGLYLGELLPAHVPLYLTYAGRIV
                     APIFFFLSVESYFKTSNRRRYIIRLFAWALAMQVGNLIISEIVKNAYHAEGSLGQNIF
                     LSIAIGISMIAAFEWGRKQSGIKKWGGFVFAGILAFFSLLTESSYNGLAMFIVFYFFY
                     NKKPALYFAYAGLCLLFFLWGLNNIEYFWEFEYQWMMIAALPFIILYNGERGPHSLKY
                     LFYAFYPLHIWALYIIRYSILAFYYPIQ"
     gene            complement(927..2013)
                     /gene="prfB"
                     /locus_tag="PLW25_06455"
     CDS             complement(join(927..1946,1948..2013))
                     /gene="prfB"
                     /locus_tag="PLW25_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113918868.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOS54164.1"
                     /translation="MEELIATLQANLTTVHSLLERLDLPQKENELAALTLKSQDPNLW
                     DDPRQAQIVMKNLADLRDEISTWKNLEKRISDALELAELADSSLQQELEAELTAITNQ
                     VANLEMATLLGGKYDRGNALLTINTGEGGTDAQDWAAMLERMYLRWCERRGFETEILE
                     QTEGEEAGIKTVSIAVNGAFAYGYLRAEKGVHRLVRLSPFDAAHRRHTSFAQVEVLPE
                     AVQEDPTVVINPDEIKIDIFRSSGAGGQNVQKNATAVRLTHLPTGIVVTCQNERSQMQ
                     NRENAMRVLRARLLDLAQIAADQHLSDLRGAYQKAEWGSQIRSYVLHPYQMVKDHRSG
                     YEVGNSLAVLDGDLDGFIEAYLRMKKE"
     gene            complement(2023..2940)
                     /gene="ftsY"
                     /locus_tag="PLW25_06460"
     CDS             complement(2023..2940)
                     /gene="ftsY"
                     /locus_tag="PLW25_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOS54165.1"
                     /translation="MSALFNKWRESLEKTRKATFGRIITVFGGSEITRETWDELEALL
                     IQADVGVETSQRIIERVSTAIHEKGLTKTNEFIQTLEETLLDLLLPSTPLELDSHHPA
                     VVTLVGVNGSGKTTTAAKLGFIYSNEGKKVLLAAADTFRAAAIDQLQVWGDRLHLPVI
                     AGQPESDPGAVAYDALQAGIARNMDIVLIDTAGRLHTRYNLMEELKKVHRVLAKALPG
                     APHATLLVLDATTGQNALHQARAFQQAVNVSGVILSKLDTSARGGMAFAIQSELGLPI
                     YYAGLGEKPEDLQRFDRYQFVKNILGVKE"
     gene            complement(2973..4016)
                     /gene="rlmN"
                     /locus_tag="PLW25_06465"
     CDS             complement(2973..4016)
                     /gene="rlmN"
                     /locus_tag="PLW25_06465"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOS54166.1"
                     /translation="MMETDKYLFDLNQDQLKDYFVSIGEPPYRVEQLWNASYTHFARD
                     FNQVTAFPVRLRSQLASDMHFSHLKTVERFASTDGETIKNLFTLPDGNLIESVLMHYN
                     ERQTLCISSQSGCAMGCVFCATGQMGFRRNLSAGEVIEQVLFLGRELSQSGEKITNIV
                     IMGMGEPFHNYDAVLEAIDRLNDPNGLNLGARRFTISTVGIVPGIRRFAAEKRQVNLA
                     ISLHAANDTLRSVLIPANRKYPLSSLMEACGEYIRLTNRRLTFEWALIRDINDSVEHA
                     RELAALLKGLLCHVNIIPLNPTTSYNGKATTEERAQEFKQVLENNGIPCTIRARRGID
                     ISAGCGQLAARRA"
     gene            complement(4003..4800)
                     /locus_tag="PLW25_06470"
     CDS             complement(4003..4800)
                     /locus_tag="PLW25_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOS54167.1"
                     /translation="MDFEKLKSVKALIVDMDGVLWRESAPIGNLPCIFNKINALGLNY
                     ILATNNATRTPQQYVHKLAGFGVKIPVEKIINSSMAVASLLAKKHPQGGAVYIVGENG
                     LIEALANAGFYPSESNCLAVIASMDRCVSFEKLKRATLLIRSGVPFYATNPDRTYPTP
                     AGLIPGAGALVGALEIATDVHPIIAGKPNPTLYEFALERLGVTPEVTLAVGDRLETDI
                     LGGQRLGCKTALVLSGIATREEAEKFTPPADFIFPDLESLLDCYDGN"
     gene            complement(4862..5467)
                     /locus_tag="PLW25_06475"
     CDS             complement(4862..5467)
                     /locus_tag="PLW25_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOS54168.1"
                     /translation="MNLPDFKNVIPMVIESSGRGERAYDIYSLLFRERIIFVGTPIDD
                     QVANAIVAQLLVLSREDPDADIQMYINSPGGQIYSGLAIYDTMKMIPNKISTVAVGVT
                     ASFGTVLLAAGTKGKRYALPHATIHMHQPLGGAEGQASDIEIQAREILRLKSRLINIL
                     AESTGQKLEVIEHDTDRNFYLDAQQAVEYGLVDQVLTPPQP"
     gene            complement(5476..6990)
                     /gene="tig"
                     /locus_tag="PLW25_06480"
     CDS             complement(5476..6990)
                     /gene="tig"
                     /locus_tag="PLW25_06480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOS54169.1"
                     /translation="MKLNIETREDHQAKIIAEFEPSDLDKYKIMAARRIASKSKIPGF
                     RPGKAPYDVVSRLYGEPAIEEDAIELMMEAVYPEILVEAKIDPASSGTLEDVDKGDPI
                     KFTFVVPLQPTVTLGNYKEIRKAYAPKSVTEKQVNEFIQHLRRTYATAEPVERPAAKD
                     DLVYIKLDATLLKPDKEENPELLKDRPLQLVIGENDPEENDYPYPGFGDNLIGLNAND
                     EKKIKYTYPKDSKFEKLRGKAVEFNVLLQTVKQLTLPELNDDFAKMFGEYETFAQLKE
                     AIKEQLQNRQTSEYDEMYYEELLDEVVKTATVKYPPQVLEHEMEHTIENVIHDLSHQK
                     MELDVYLKTINKEKEVWLEEEVKPAAVKNLSKSLVLRELAKAEDIELASEEIQTEVTG
                     MLTQIQQTTNPKALSKQLKNKNYLNALTMDAASRAMSRKVFERLKDIATGKTAEVAGK
                     PVKAARSKSAKEAKDSVQSTDEQKPQAKKTKAAAAKEVTPATVKKTKKVEKTSE"
     gene            complement(7199..7447)
                     /locus_tag="PLW25_06485"
     CDS             complement(7199..7447)
                     /locus_tag="PLW25_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54170.1"
                     /translation="MKRENGKIAIIFILIILVILAVLLFALAPQNVKVTDPLFIVQYW
                     QNITRALANLGGFLSRFLQPIGEGIVNFFSNIGLPKIK"
     gene            complement(7502..7583)
                     /locus_tag="PLW25_06490"
     tRNA            complement(7502..7583)
                     /locus_tag="PLW25_06490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7547..7549),aa:Leu,seq:tag)
     gene            7691..8098
                     /locus_tag="PLW25_06495"
     CDS             7691..8098
                     /locus_tag="PLW25_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54171.1"
                     /translation="MSKIYSPDSAGKERARLSKTIVLALRELMKQSEPDNLTKDITAY
                     VILALEEIAANIDTSVEAWEKRGYWVKADRFRLEWEWAGNSASRLRIAFLSEDWGAIA
                     VTTAYIGQKLNKVVVPQRHRLGTPWVGAARNLK"
     gene            complement(8146..10257)
                     /locus_tag="PLW25_06500"
     CDS             complement(8146..10257)
                     /locus_tag="PLW25_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOS54172.1"
                     /translation="MDKTGIEKMLRPKSVAVIGASATPGKIGYTVIKNLLKDGYKGKI
                     YPINQKESEILGLKCYPEIGQVPGEIDSAVMCVPAKVMAEVVEECGRKGVKGALVITS
                     GFSEIGRKDLEDEVVRIARHYGMRILGPNIVGTLSNSEKFNGSFAPCLPLPGKNSLVT
                     QSGALLIAMDMATYSRRIGYEKMISIGNMADVDFADLIEWFQDDEETSCVSLYIEGLR
                     DGRRFIDVAKKSNKPIIALKSGVSAHGAAAAASHTGSLAGGAKVYSAAFKQAGVIQAT
                     DLDNLFYRGLTLSMQPPMKGDNFLIITNGGGNGVLATDAAERFGIPLKFAPDDVQAEM
                     KKHMPEYGSAKNPVDMTGMAGNDWYYETVKFAIMNKWTDSVVVLYCETAITDPAEIAA
                     SIKRAVDDSGVKDKPITVSFVGGEKSDKAIGWMVENGIPAFFAPDTAVNAMAALREYA
                     KLQENKKSDGAKTDEKAKKLALEVIAAARADGRTSLTEIEAKKVFELYGMPTTKIGLA
                     KTEEEAVKLSKEIGYPIVMKIVSPDILHKSDAGGVKVNIKTEAEAREAYNTILKNAKA
                     YKADANIHGIAVQEMAPWGTEVILGSVSDPSFGPTVMFGLGGIFVEVLKDVTFRVTPV
                     SKAEAEEMVSEIRGAPILSGVRGEAPRDRKAIIETIIKYSSMIYDLMDEIAESDANPV
                     LVYAQGEGLRVADARIILKKK"
BASE COUNT         2494 a         2586 c         2617 g         2832 t
ORIGIN      
        1 cactattgaa taggataata aaaggcgagg attgaatatc ttattatata taaagcccaa
       61 atatgcaaag ggtagaaggc atagaataaa tatttcaaac tgtgaggtcc gcgttcacca
      121 ttataaagga tgataaacgg taaagccgca atcatcatcc attgatactc aaactcccag
      181 aagtactcga tattattaag accccacaag aaaaagagaa ggcacaaacc cgcataggca
      241 aaatataagg caggtttttt gttgtaaaag aaataaaaaa cgataaacat tgcaagaccg
      301 ttataggatg attctgttaa aaggctgaaa aacgccaaaa ttcctgcaaa aacaaaacca
      361 ccccattttt ttatcccgct ttgtttgcgc ccccattcaa atgcggcaat catacttata
      421 ccgatggcaa tggatagaaa aatgttctgc cctaaagagc cttcggcatg atacgcattt
      481 tttacaattt cgctaatgat aagattgccc acttgcatgg ccaatgccca tgcaaacaac
      541 cgaataatat aacgacggcg attactggtt ttaaagtaac tttccacgga aaggaaaaag
      601 aaaatcgggg caacaatgcg cccagcgtat gtaagataaa gcggaacatg tgcaggtaac
      661 aattcgccca aatagagacc gaaatgctct ccgaacatca gaacaagcgc aatcaatttg
      721 agggcaaagc catttaattt catcaattct ccttgattat ttctgaacca ggcacccgac
      781 gagactgccg aaaaacgatc tctgaatttt tgaactgtgc aggttcttca tttgcaagtt
      841 ggtcttatcc agcttggttt tactccatct tgaacagatt cgattatcca agccgatagt
      901 gataggattg aataaaaaaa ccgccgttac tcttttttca tgcgcaaata cgcttcaata
      961 aagccgtcca ggtcgccgtc aagaacagcg agagagttgc ccacttcgta gccgctgcgg
     1021 tggtctttca ccatctggta tggatgcagc acatatgacc gaatctggct gccccattcg
     1081 gccttctggt atgcgccgcg cagatcagaa aggtgctggt cagccgcaat ctgagccagg
     1141 tcaagcaagc gcgcccgcag cacacgcatg gcgttttcgc ggttttgcat ttgagaccgt
     1201 tcattctggc aggtgaccac gatccccgtg ggtagatggg taaggcgcac ggcagtggca
     1261 tttttctgca cgttctgtcc tccggcgcct gatgatctga aaatatcgat ctttatctcg
     1321 tcaggattaa tgaccacggt tgggtcttct tgcaccgctt ctggcagtac ttctacctgg
     1381 gcgaaggaag tatggcggcg gtgggccgcg tcaaaaggtg ataacctcac cagccggtgg
     1441 acgccttttt cagcccgcag gtatccatat gcaaaagcgc cgttcactgc aatgctcacc
     1501 gttttaatac cagcctcttc tccttcagtt tgttccagaa tctcagtttc aaaaccacgc
     1561 cgttcacacc agcgcaggta catgcgttcg agcatggcag cccaatcttg cgcgtctgtg
     1621 cccccttcac ccgtgttgat ggtcaagagc gcgtttcccc ggtcatattt cccgcccagc
     1681 agggtggcca tttcaaggtt agcgacttgg ttggtgatgg cggttaattc cgcttcaagt
     1741 tcttgctgca gcgaagagtc agcaagttcc gccagttcta acgcatcact gatgcgcttc
     1801 tcgaggttct tccaggtgga gatttcatct ctcaaatcag ccaggttctt cataactatt
     1861 tgcgcctgcc tgggatcatc ccaaagattg ggatcctggc ttttcagtgt taaagcggcg
     1921 agttcatttt ctttttgagg taagtcaaag acgctccagg agagaatgta ctgtcgttaa
     1981 attggcttgc aaggtggcaa ttaattcttc catgaatgaa ccctactctt ttaccccaag
     2041 gatgtttttt acgaattgat agcggtcgaa gcgttgtaaa tcttcaggct tttcgcccag
     2101 acccgcgtag taaattggta atccaagctc gctttgaatt gcaaaagcca ttccaccgcg
     2161 ggctgaagta tccaacttgg aaaggatgac cccgctgacg ttcaccgcct gttgaaaggc
     2221 gcgcgcctgg tgaagggcgt tttgtccggt cgtggcatcc agcaccagca gggtggcgtg
     2281 tggcgcgcca ggaagagcct tggcaagcac tctgtgcacc ttcttcagtt cttccatcag
     2341 gttataacgg gtatgcaggc gccccgcggt atctatgagc acgatatcca tgttccgcgc
     2401 gatgcccgcc tgcagcgcat catacgctac tgctccaggg tcgctctctg gttgaccggc
     2461 gatgactggc aggtggaggc gatctcccca aacttgcaac tggtcgatgg cagcagcgcg
     2521 aaaagtgtcc gctgctgcta aaaggacctt cttcccttca tttgagtaga taaaaccaag
     2581 ctttgccgcg gttgtggttt tccctgagcc gttgacgccc accaaagtga ccacagcagg
     2641 gtggtgagaa tccagctcca gtggtgttga aggcagaagg agatccaaca gggtctcttc
     2701 caatgtctgg atgaactcat ttgttttggt aagacctttt tcatggattg cagtactgac
     2761 acgttcaatg atacgctgac tggtttccac gcccacgtct gcctgaatca acagggcttc
     2821 cagttcatcc caggtttctc tggtaatctc cgacccgcca aacacggtaa taattctgcc
     2881 gaaggtggct ttgcgagttt tttcgaggct ctcacgccat ttgttgaata aagcgctcat
     2941 cgcttgcgat tataacatgt ccgttttaag cgttacgcgc gcctggctgc cagttgacca
     3001 caccccgcgg aaatatcgat tcccctgcgc gccctgatgg tgcaggggat gccgttgttt
     3061 tccagtactt gcttgaactc ttgggcccgt tcttcggtag ttgcttttcc attgtaactg
     3121 gtcgtgggat tcagcgggat gatgttcacg tgacacaaca gccccttgag taaagcggcg
     3181 agctcacgcg catgttcgac ggagtcgttt atatctctga tcaaagccca ctcaaaagtg
     3241 agccggcggt tggtcagcct gatatattcg ccgcaggctt ccatcagcga ggaaagtggg
     3301 tacttgcggt tggctggaat gagcacgctg cggagtgtgt cgtttgctgc gtgcaggctg
     3361 atggcgaggt taacctgccg tttttcagcc gcgaacctgc gaataccagg gacgataccc
     3421 actgtggaaa tggtgaagcg ccgcgctccc aggttcaaac cgtttggatc attcagccgg
     3481 tcgatggctt ctaaaaccgc atcgtagtta tgaaacggtt ctcccatacc catgatgaca
     3541 atgttggtaa tcttttcacc actttgggat aactcacgac ccaggaacag gacctgttca
     3601 atcacttcac cagcgctcag attgcgcctg aagcccatct gaccggttgc gcagaaaaca
     3661 cagcccatag cgcaaccgga ttgcgacgag atgcacaacg tctggcgctc gttgtagtgc
     3721 atcagaacag attcgatgag gttaccgtca gggagggtaa agagattttt gatggtttca
     3781 ccatctgtag aagcgaacct ctctaccgtc ttgaggtgtg aaaagtgcat gtcgctggca
     3841 agctgcgaac gcaggcgcac agggaaggcg gttacctggt tgaaatctcg cgcgaagtgc
     3901 gtataggaag cgttccatag ttgctcaacc cggtaaggtg gttcaccaat ggatacgaaa
     3961 tagtccttca gttgatcctg gttgagatca aacaaatact tatcagtttc catcataaca
     4021 gtctagcagg ctttcgaggt cagggaagat gaaatctgca ggcggggtga atttttctgc
     4081 ctcttcccgt gtggcgatgc ctgaaaggac aagcgcggtt ttacaaccca atctttgacc
     4141 gcctaaaatg tcagtttcaa ggcggtcacc tacggccagg gtgacttctg gtgtaacccc
     4201 caacctttca agcgcgaact catacagggt gggatttggt tttccggcga taattggatg
     4261 cacgtcagtg gcaatttcaa gcgctccaac cagcgcgccg gctcctggaa tcaacccggc
     4321 tggggtgggg tatgttcgat cggggttggt agcgtaaaaa gggaccccgc ttctgatcag
     4381 cagggtggcg cgtttgagct tctcgaatga gacgcatcga tccatgctgg cgatgacagc
     4441 caggcagttg gattcggatg gataaaaacc agcattggcc agggcttcta tcaagccgtt
     4501 ctcgccaacg atataaaccg ctccgccctg ggggtgcttt ttggcaagca gggatgctac
     4561 ggccattgat gaattaataa ttttctcgac aggaattttt acaccaaagc cagcgagttt
     4621 gtgcacatat tgctgtgggg tgcgggttgc attattggtt gccagaatgt agtttaaccc
     4681 cagtgcattg attttattaa agatgcacgg taagttgcca attggcgctg attcgcgcca
     4741 aagaacacca tccatgtcta ctattaatgc tttgacactt ttcagttttt caaaatccat
     4801 aaatcctcat aaaacgagcc ggatagcggc tgccacccgg ctcgagtgaa cgagtgcctt
     4861 gtcagggctg tgggggggtg agcacctgat ccaccagacc gtattcaacc gcctgctgag
     4921 catcgaggta gaagttgcgg tcagtgtcgt gttcgatcac ttccagtttc tgaccggtcg
     4981 attcagcgag gatgttgatc agcctggatt tcaaacgcag aatctcgcgg gcctgaatct
     5041 caatgtcgga ggcctgccct tcagcaccgc cgagtggttg gtgcatgtgg atagttgcat
     5101 gcggtaatgc gtagcgtttt cctttggtcc cggctgcgag caacaccgta ccaaaagagg
     5161 cagtcacgcc cacggcaacc gtgctgatct tgtttgggat catcttcatg gtgtcgtaaa
     5221 tggccagccc ggagtaaatt tgaccgcctg gcgagttgat gtacatctgg atatccgcat
     5281 cgggatcttc tcgacttaat accagcaact gcgccacaat ggcattcgcc acctgatcgt
     5341 caatgggggt gccgacgaag atgatgcgct cacgaaaaag cagtgaatat atatcatacg
     5401 cgcgctcgcc acggccggat gattcaatga ccatggggat gacgtttttg aaatcaggta
     5461 aattcatttt ttctcctatt cgcttgtttt ctcaaccttc tttgtttttt taacggtagc
     5521 aggcgtaact tcttttgcag cggcggcttt ggttttcttc gcctgaggtt tttgttcatc
     5581 agttgattga actgagtcct ttgcttcttt tgcgcttttt gaacgagcgg cttttacggg
     5641 tttaccagcc acttcagctg tttttccagt ggcgatgtct ttcaaacgtt caaacacttt
     5701 tcggctcatt gcgcgcgagg ccgcatccat tgtcagcgcg ttgaggtagt ttttattttt
     5761 tagctgcttg ctgagggctt ttgggttggt ggtttgctga atctgagtaa gcattccagt
     5821 gacttctgtt tgaatctcct cactggcaag ttctatatct tcagccttgg ccagttcacg
     5881 caaaacgagt gacttggaga ggtttttcac tgccgctggc ttgacttctt cttccagcca
     5941 aacctccttt tctttattga ttgttttcag gtacacatcc agttccattt tttggtgaga
     6001 gaggtcatgg atcacgtttt cgatggtgtg ttccatctca tgctcaagga cctgcggcgg
     6061 gtatttcacg gtagcagttt tcaccacctc atccaacagt tcttcataat acatctcgtc
     6121 atattcactg gtctgacgat tctgcagttg ctccttgatg gcttccttga gctgcgcaaa
     6181 ggtttcgtat tctccaaaca tcttggcgaa atcatcattc aattcaggca gggtgagttg
     6241 ttttactgtc tggagaagaa cgttgaattc cactgcttta ccgcgtaatt tttcgaactt
     6301 ggaatccttg ggataggtgt acttgatttt cttttcgtcg ttggcgttta acccaatcag
     6361 gttgtcgccg aagcccgggt acgggtagtc attttcttca gggtcatttt cgccaatcac
     6421 cagttgcaag ggcctatctt ttaataattc cgggttttct tctttgtcag gctttaagag
     6481 ggtggcatcc agtttaatat agaccaggtc gtctttggca gcaggacgtt caacgggctc
     6541 tgctgtggcg taggtgcggc gcaagtgctg aatgaactca ttgacctgct tttcggtaac
     6601 ggatttgggc gcataggctt tgcggatttc cttgtaattt cccagggtta ctgtcggttg
     6661 gagaggaacg acaaaggtga atttgattgg atcaccttta tccacatctt cgagggtgcc
     6721 agaagaagcg gggtctatct tggcttcaac cagaatctcg gggtatacgg cttccatcat
     6781 taactcaatg gcgtcttctt cgatcgcggg ttctccgtag agtctggaga ccacatcgta
     6841 aggcgctttt cccggacgga aaccgggaat tttgctcttg gaagcgatgc ggcgggcggc
     6901 catgatttta tatttatcca aatcgctcgg ttcaaattca gcaatgatct tagcctggtg
     6961 atcttcacgt gtttcgatat ttagtttcaa cttccttatc cttttcaact ttttttctgg
     7021 cagagggagt gagaactccc gctggcgttg cgtctatgac cccggtcatt gatggcggca
     7081 agtaaaaacg cgtcccaatt atactcaaaa tcctgcgatt aagataaaaa acgcactctc
     7141 gctggagttt catataaagt tctaataagc tcgggggata ttggggaaaa ggttcaggtt
     7201 attttatttt tggcagcccg atatttgaaa agaagttaac gataccctct ccgatgggtt
     7261 ggagaaagcg tgaaagaaaa ccgccgaggt tcgcgagcgc cctggtgatg ttttgccagt
     7321 actgaacaat gaataaagga tcggtcacct tgacgttttg cggcgccaga gcaaagagca
     7381 acaccgccag aatgaccagg atgatcagga tgaagatgat tgcgattttg ccgttttctc
     7441 ttttcatagt gttcagctta tcatggttgg acaacaatac ttcgcttgaa tcgtctgcaa
     7501 gtggggaaag cgggacttga acccgcacgc cttgcggcac atgatcctaa gtcatgcctg
     7561 tctgccaatt ccagcatttc cccggtgtgc gctgaaatta taagcgctgg tctggcaggc
     7621 gtcaataatc gaatataatc aagcacgttc gatgcgggta taaaaatgca cgagaaggag
     7681 cgtacctctt ttgtcaaaaa tttattcacc ggatagcgcc ggaaaagaac gcgccagact
     7741 ttcgaaaaca atcgtgctgg ctttacgcga acttatgaaa caatcggagc cggataacct
     7801 taccaaggat atcactgcct atgtgatact cgccctggaa gaaattgccg cgaatattga
     7861 cacctcggtt gaagcctggg agaagcgggg atactgggtg aaagcggatc gtttcaggct
     7921 ggaatgggaa tgggcaggta atagcgcttc aaggctgcga attgcttttt taagcgaaga
     7981 ctggggcgcg attgcagtta caactgctta tatagggcaa aaactcaaca aagtggtggt
     8041 gccgcaacgc catcgcctgg gcacaccctg ggtgggggcg gcaagaaatt taaaataaaa
     8101 aagagcttct gcttgttggc aaaagctctt tccgcgggtg atcagttact ttttcttgag
     8161 gatgatcctg gcatcagcaa cgcgcagtcc ttcgccttgc gcgtaaacca gcaccggatt
     8221 ggcatcagat tctgcaattt catccatcag gtcatagatc atgctcgagt atttgataat
     8281 ggtctcgatg atggctttac gatcgcgagg ggcctcgccg cgaacacctg agaggattgg
     8341 ggcaccgcga atttcactca ccatctcctc cgcttcggct tttgataccg gggttacacg
     8401 gaaggtgaca tctttgagca cttcaacgaa gatgcctccc agtccgaaca tcacggttgg
     8461 gccgaaggat gggtcactta ccgaacccag gataacctcc gtgccccagg gcgccatttc
     8521 ctgcacggcg ataccatgaa tattcgcatc cgctttatac gctttggcgt tcttgaggat
     8581 ggtgttataa gcctcgcgag cttcagcttc ggttttaata ttgactttga caccgcctgc
     8641 atctgatttg tgaagaatat ctggcgagac aattttcatc acgatggggt aaccgatttc
     8701 tttactcaac ttgacggctt cttcttcggt tttcgccagt ccaatcttgg ttgttggcat
     8761 accatataac tcaaagactt tcttggcttc aatttcggtc aatgaagtac gcccatcagc
     8821 tcgcgcagcc gcaatgactt caagcgccag ttttttggct ttttcatcgg ttttcgcgcc
     8881 gtcagatttc ttgttctctt gtaatttggc gtattcgcgc agggctgcca tcgcgtttac
     8941 cgctgtatca ggggcgaaga acgccgggat gccgttttcc accatccaac cgatggcttt
     9001 atctgatttt tcaccaccga cgaaagatac cgtgatcggt ttgtctttta cgccactgtc
     9061 atccacagcc cgtttgatgc tcgcagcgat ttctgcaggg tcagtaatgg ctgtttcgca
     9121 gtacagcaca actacactat ccgtccactt attcataata gcgaacttga cggtttcgta
     9181 ataccaatcg ttgcctgcca tgccggtcat atccacgggg ttcttggcag aaccgtactc
     9241 gggcatatgc ttcttcattt cggcttgaac gtcatccggg gcgaatttca acgggatgcc
     9301 gaaccgttcc gcggcgtcag tagcgagcac accgttaccg ccaccattgg tgataatcaa
     9361 gaaattgtcg cctttcatcg gcggctgcat ggagagagta aggccgcggt agaagaggtt
     9421 atcaagatcc gttgcctgaa tcacacctgc ctgtttaaaa gctgcgctgt acacctttgc
     9481 gcctccggcg agcgagccag tgtgagaggc tgcagccgcc gcgccgtgag cagaaacgcc
     9541 agacttgagc gcgatgatgg gcttgttgga ttttttggcc acatcgataa acctgcgccc
     9601 gtcccgaaga ccttctatgt agagagaaac gcaggaggtt tcctcatcat cctggaacca
     9661 ttcgatcagg tcggcaaaat caacatcggc catgttgccg atagatatca ttttttcgta
     9721 tccaatccgg cggctgtaag ttgccatgtc catggcaatt aacaacgcgc ctgattgggt
     9781 gaccagtgag ttcttgcccg gcaaaggaag gcagggcgcg aatgacccat taaacttttc
     9841 ggaattggag agcgtgccaa cgatgttggg gccgaggatg cgcataccat agtggcgagc
     9901 gattctgacc acttcatctt ccaggtcttt tcggccaatc tcgctaaacc ctgaagtgat
     9961 taccagggcg ccttttacgc ctttacggcc gcattcttca actacttccg ccataacttt
    10021 ggctggaacg cacatgacag cagagtcgat ctcgcccgga acctgcccaa tttccgggta
    10081 gcacttcaaa ccgagaatct cgctttcttt ctggttgata gggtaaattt tgcccttgta
    10141 accgtctttc agcaggttct tgatgacagt gtacccgatc ttccccggtg tagccgaagc
    10201 gccaatcacc gcgacagatt tcggtctgag catcttttca attccagttt tatccacagc
    10261 aaattcctcc ttttggattg atcaatcagg ttgtcttgaa caaatgatag aataattcaa
    10321 ctgtcatttt acccactttt cgcaaaacaa aagtcctcaa atctatgaat attcatcata
    10381 aaccaaccta tgaagaaaga atctccgctg cgctgaacaa caccgccacg ccactcgcaa
    10441 gaaaaggtga tttgatcaag attctctcgg cagcactgaa ggcagttgat ccgcagtcgg
    10501 tagtgagaaa taatttgtgg caaatcggg
//
LOCUS       DAOSPM010000063        10343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_6455_length_10343_cov_13.093507, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000063 DAOSPM010000000
VERSION     DAOSPM010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6455_length_10343_cov_13.093507"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PLW25_06505"
     CDS             complement(<1..659)
                     /locus_tag="PLW25_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS54173.1"
                     /translation="MMPNIIVDRISKAFGNLQAVKNVSFEVNPGEIFGLLGPNGAGKT
                     TSIRIILDIFKADSGTVSILGGAMTEEKKNRIGYLPEERGLYQDIQLERCLVYLATLK
                     GLEESEARGRINDYLQKFDLADARKKKVKELSKGMQQKAQLITTLVHKPEVVIIDEPF
                     SALDPVNTQMVKDLLKEERHRGTTIVMCTHQMHQVEELADRIALINAGETVLYGGLTE
                     IR"
     gene            complement(752..2029)
                     /locus_tag="PLW25_06510"
     CDS             complement(752..2029)
                     /locus_tag="PLW25_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS54174.1"
                     /translation="MAATSTSIQRVRISDISPARKISTGVVEVLVALFIFAVFARTVS
                     AGSITTFVMTPGGITTGQMGNWVIPSQLTLYLLAALSLVIGLFQLIRGFGKITNLMVG
                     LVFLFFIFSFLTWQAADKSLNLAGMLSSAVQLAVPITMGSFSGILCERSGVVNIAIEG
                     MMLMSAMVGALMGSLTHSAWLGLFFGVLSAVLLAALHAVLSIKYKIDQIISGTVINMF
                     SAGMTAFLSQKFLQTRQELNNPAMFTRIEIPGLAEIPLIGPILFNTNFFVYLMFALLI
                     ILQVALFSTRWGLRVRSVGEHPKAADTLGINVIRTRYMSVLLGGMVAGMAGAFFTLGS
                     VGRFEEGMTAGKGFIGLAAMIFGNYTPIGALGAGILFGFADSLGSKLSLLGSAIPSTL
                     MSTAPYVITMIVLAGFVGKGHVPAADGVPYTKE"
     gene            complement(2029..3312)
                     /locus_tag="PLW25_06515"
     CDS             complement(2029..3312)
                     /locus_tag="PLW25_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS54175.1"
                     /translation="MGKKDLEKQDIGKTILEELTGGEPKEKRQKRGIWKILQIPALAI
                     LTGLIIGAVLIMATSFDVYDAFKVSLWKGLGTAFAEVGEAYLALITGSLGNPVEIVQA
                     LLYGDALDFRNAINPILESLVQSTPYIFAGLACALGFRAGLFNIGVEGQLFIGAAAAT
                     FVGYSLTGLPPYIHMPLAFLAGALGGALWGMVPGLLKATTGGHEVINCIMMNYIAYRF
                     TTFLLTGPMSRPGTGGMPVSPIIEKSAQIPQFFKTPIRFHLGFFIALAFAALVWWVLF
                     KTTWGLNLRTVGTNPRAAKYAGMNIIATTIIGMAASGALAGMAGANEVLAVNRSMAIG
                     LSAGYGFDSIALALLGNSHPVGVIFAALLFGVLKNGATKMMVVSTIPIDIVTILQAVI
                     LIFVAAPAIIRSVYRLKQPKVEAAAIMLSGWGGVK"
     gene            complement(3325..>3558)
                     /locus_tag="PLW25_06520"
     CDS             complement(3325..>3558)
                     /locus_tag="PLW25_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HOS54176.1"
                     /translation="KRIVQKRDEGCAVLLVSPELDEVIELSDRIAVMYRGKIIALLEA
                     EEVAKETLGLLMAGIVPDKIEKPQGQRVVETTF"
     assembly_gap    3559..3658
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3659..4555)
                     /locus_tag="PLW25_06525"
     CDS             complement(<3659..4555)
                     /locus_tag="PLW25_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS54177.1"
                     /translation="MAPILQLKGITKQFPGVLANDHIDLTLNQGEILALLGENGAGKT
                     TLMNILYGLYQPDEGEILVNGEKITIHSPTDAINAGIGMVHQHFMLIPVFTVTENVML
                     GSEPVKAGDFLDRPKTATKIMEISSQYKLEVDPNDYVKDLPVGVQQRVEIIKLLYREA
                     NILIFDEPTAVLTPQEADDLFVIMRSLVKQGKSIIFITHKLREVLDVADRIMVIRRGA
                     VVGEADPKQANRNKLAEMMVGREVNLVVEKDIKKPGDVILSVKDLVVTDQFKQVMVDK
                     VSFEVHAGQILGIAGVQGNGQTE"
     assembly_gap    4814..4913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4914..5563)
                     /locus_tag="PLW25_06530"
     CDS             complement(<4914..5563)
                     /locus_tag="PLW25_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOS54178.1"
                     /translation="MSKKVMLVMSVLLIAVFVLSACAKTAGPAKFKACEVTDTGGVDD
                     KSFNATAWKGAVDAKEKLDIEAKYLESKEIADFEKNINAFIAEKCDIIITVGFLLGDA
                     TKAAAEANPNQKFSIVDYGYDPVIPNVLAQAFQTDEAAYLAGYAAAGFTKTGKVGTFG
                     GMQIPTVTIFMDGFARGVRHYNEVHGTSVETLGWDADAQTGLFTNDFSDQQKGSDMA"
     gene            complement(5704..7026)
                     /locus_tag="PLW25_06535"
     CDS             complement(5704..7026)
                     /locus_tag="PLW25_06535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS54179.1"
                     /translation="MEKKKFPWGRTFLVGFGFFGISILWPIFNQWVPLILQAGNPGFK
                     TNIPNFVGFGIGPALAMFIMTWDNIVNIFVQPWVGNRSDHTWNRFGRRKPWILLGLPI
                     ALVGFISIPLAQSVLAVAVFIMVTNVGMALFRSPVVSWLGDLFLPEQRSKANGVINLM
                     GGAAGVLSYVLGGLIFNAFGRMGPFVAGAVAMTVALAAVLIWVKEPKERLGKHTEDED
                     SSGAVKGLVPNLKAVFSNQDKSTLFILLAIFCWFTGFNAIETGLSSFAVFDLGIAPGK
                     ASIVASIANLSFLAFCIPSGILAGRLGRKRTILYGVFALTLIFLISFFLVKGLISFII
                     ILVLVGFFWSWVNVNSLPLVYDHGDEKRIGAYTGLYYFFSQTAAVLGPTLGGLLVERI
                     HFNYKVLFIFGAVFLFLAFLSMLKVKDRAHEEKMKLGAGIGASSSASE"
     gene            complement(7030..7743)
                     /gene="trmD"
                     /locus_tag="PLW25_06540"
     CDS             complement(7030..7743)
                     /gene="trmD"
                     /locus_tag="PLW25_06540"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOS54180.1"
                     /translation="MQFDVFTLFPGVLRPYLDSSILQRAQQNDLIKVALHNIRDWTFD
                     RHHVTDDAPYGGGGGMVMKVEPIFAAVESVLGAPPSCPLLYLSPQGKPFTTAIARELA
                     QQPRLGLLCGRYEGVDERVIEHLVTEQISIGDYVLTGGELPALVLIDAVSRFIPGVLG
                     DPDGAEEDSFGNGLLEYPHYTRPEEFRGWRVPEVLISGNHALVAKWRREQSLLRTQKH
                     RPDLLNGESKVQSTTKTKE"
     gene            7932..9290
                     /locus_tag="PLW25_06545"
     CDS             7932..9290
                     /locus_tag="PLW25_06545"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS54181.1"
                     /translation="MIAQRGEGGLPVILTLLVSLGKMNSMYIKGSKWSMQKKKRRSNP
                     FRVILLIVLIAAAIYFDRMIVPTIPQFFLPTPTPTRAPESITSDAKGLEAEGKYPLAI
                     VAYKDAVRADPRNPHNFIALARLNIYTGNYEEAVTDAENALLLNPNNDEAMALRGWAI
                     GLTGDYVRGTGILQEAIAANPDYAAAYAYLAEIYTYMLQAGQGDLQTLDNAINASRRA
                     EELAPGTLETHRGRGMVLEYTSNFKEAVAEFEAAVALNPNIADLHLGLGRNYFNIQEY
                     DDAITEFGRANALNPRDPTPESLIARTYALNGDYQKAIQYGEAAIMDEPQNARLYGNL
                     GLIYSSAKQYLDAIDMLRIAVRGGVSSDGAPVEGIPMDYGRVAQYYYTYGLALARVGQ
                     CGEALEISQAVQMGLRNDDIAVFNAEEMVNICERLAREGKSDIAATATPAETTPTVEA
                     AP"
     gene            9319..>10343
                     /locus_tag="PLW25_06550"
     CDS             9319..>10343
                     /locus_tag="PLW25_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS54182.1"
                     /translation="MIDYSGKRALFGHPRKRSNPIWIFTLLLLILAGLFVIQAVRDGR
                     IAPILQIIPTPTRSLNSYVQEGETHFQAGNLEKAIEAYRYAIAIDPEDADLRAELARI
                     QVYSTSQITVDARKRERLEEALATIEEGLAISPQNSNLYAVKAFALDWYGSSGAAGEA
                     SQEYLVKGEQAATEALQYDNTNALALAYYAELLIDQLKYSQAQQYITQALERDDTLMD
                     VHRVNGNVQEASANYTQAIEEYKKAIQITPNLNFLYLSLGANYRQLANMTGITTEQNY
                     YFGLALEAFAKAVAINDQLGVRDPIPLISIANTYVQQGEFFAAARNMLRAVAFAPDDP
                     TAYGQLGV"
BASE COUNT         2491 a         2672 c         2725 g         2255 t
ORIGIN      
        1 cgaatttccg tcaacccgcc atataagaca gtttctcccg cattgatcag cgcgatacgg
       61 tccgccagtt cttccacctg gtgcatctgg tgggtgcaca tgacgatggt cgtaccgcgg
      121 tggcgttctt ctttgagcag gtctttcacc atctgcgtgt tcaccgggtc gagcgcgctg
      181 aagggttcat cgatgatcac cacttcaggt ttgtgcacca gcgtggtgat cagctgagct
      241 ttttgctgca tacctttact gagttcttta acctttttct tgcgcgcatc agccaggtcg
      301 aacttttgca gatagtcatt gatgcgtccc cgcgcctcgc tttcttcgag ccctttcaga
      361 gtggcaagat aaacgagaca gcgttccagc tggatatcct ggtaaagccc gcgttcttca
      421 ggcagataac cgatacgatt tttcttttct tcggtcatgg cgccgcccag gatactcaca
      481 gttccggagt ctgctttaaa aatatccagt atgatacgga tggaggtggt cttgcctgcg
      541 ccgtttggtc cgagcagacc aaagatttcg ccaggattga cttcgaaaga gacgtttttt
      601 accgcctgga gattgccaaa agccttggaa atgcggtcga cgataatatt gggcatcatg
      661 tacctttctg tttattgtgg tgattataac aaaaccggtc ttccttataa tatacgatgg
      721 aagaccggtg caggtttctg agttatcaag cttattcttt ggtgtacggc accccatccg
      781 ccgcggggac gtgccccttg ccgacaaacc cggcgagaac gatcatggtg atcacatagg
      841 gcgcggtgga catcaatgtt gaggggatgg cgctccccag taaagatagc ttggagccca
      901 aagaatcggc aaaaccaaaa aggatgcccg ctccaagcgc tccgatgggg gtgtagttac
      961 caaagatcat ggcggccagg ccgataaagc ctttccccgc ggtcatgcct tcctcgaagc
     1021 gcccgacaga ccccagagtg aagaacgctc cagccatccc agccaccatc ccgcccagca
     1081 ataccgacat gtagcgggtt ctgatgacgt tgatgcccag tgtatcggct gccttgggat
     1141 gctcgcccac actgcgcacg cgcaaccccc agcgagtgga gaagagcgcc acctgtagaa
     1201 taataagcag tgcgaacatc aggtagacaa aaaagttcgt gttaaacaga atcggaccaa
     1261 ttaaggggat ttccgccaga ccggggattt caattcgggt gaacatggca gggttgttca
     1321 gctcttgcct ggtttgcagg aacttttgag aaagaaaagc ggtcataccc gcggagaaca
     1381 tattaatcac cgtgccagaa atgatctggt caatcttata tttgatcgac aggaccgcgt
     1441 gcagggcagc cagtaaaacc gcagaaagaa cgccgaagaa taaaccgagc cacgcgctat
     1501 gcgtgaggct gcccatcaag gctccgacca tggcagacat tagcatcatg ccttcaatgg
     1561 cgatgttgac cacgcctgag cgctcgcata agataccgga gaaggaaccc atggtgatag
     1621 gcacagccag ctgtactgcg ctggaaagca taccagcgag gtttaaggat ttatcagccg
     1681 cttgccaggt gaggaaactg aaaatgaaga acaagaacac caatccaacc atgagattgg
     1741 tgattttccc gaacccgcgt atcaactgaa aaagtccgat caccaggctg agcgcagcga
     1801 gcaggtacag cgtaagctgg ctgggaatta cccagttgcc catctgacca gtggtgatcc
     1861 cgcccggggt catgacaaaa gtggtgatgc tgcccgcaga gacggttctg gcaaagacgg
     1921 caaagataaa caaggcaact aaaacttcga caacaccggt gctgatctta cgggcagggg
     1981 agatatcaga aatgcggaca cgttggatgg aagttgatgt ggctgccatt atttaactcc
     2041 tccccaaccg ctgagcatga tggcagcggc ctccactttt ggttgtttaa gccgatagac
     2101 tgaccgaatg atcgcaggag cggcgacgaa aataaggatg actgcctgca ggatggtgac
     2161 aatatcaatg ggaatggtgg agaccaccat catcttggtg gcgccattct taagcacgcc
     2221 aaataaaagg gcagcgaaaa tgacaccaac cggatgactg ttgccaagca gcgccagggc
     2281 gatgctgtca aaaccataac cagccgagag tccgatagcc atgctgcggt tcaccgccaa
     2341 aacttcattg gcgcctgcca taccggcgag cgcaccggag gcagccatac cgataatggt
     2401 ggtggcgatg atgttcatac ccgcgtactt ggcggcgcgc ggattggtgc ccaccgtgcg
     2461 caggttcaat ccccaggttg ttttaaacaa cacccaccaa accagcgcgg cgaatgccag
     2521 cgcaatgaag aaccccaggt ggaaacggat aggcgtcttg aagaactggg gaatctgggc
     2581 ggatttctcg attatcgggc ttacgggcat accaccggtg cccgggcgag acatggggcc
     2641 agtgagcagg aaggtggtga accgataggc gatatagttc atcataatac agttgatgac
     2701 ttcgtgaccg ccggtggtgg ctttgagcaa cccggggacc atcccccaga gagcgccgcc
     2761 caatgcccca gcgaggaaag ccaacggcat gtggatataa ggcggtagac cggtgagcga
     2821 gtaacccaca aaggtagccg cggcagcgcc gatgaataac tgcccttcaa cgccaatatt
     2881 gaacaggccg gctctgaacc ctaaggcgca ggccagtccg gcgaagatgt aaggcgtgct
     2941 ctgaaccagg ctttcaagaa tcggattgat ggcgtttctg aaatccagcg cgtcgccgta
     3001 gaggagggct tggacaatct ccaccggatt tcccagtgaa ccggtgatga gcgcgaggta
     3061 agcttctccc acctctgcga acgcggtacc caggcctttc cacaggctga ctttgaacgc
     3121 gtcatagaca tcgaagctgg tcgccatgat gagaaccgcg ccaatgatca atccagtcag
     3181 gatagccagc gcagggatct gcagtatctt ccagataccg cgcttctggc gcttttcttt
     3241 tggttctcca cctgtcagtt cttccagaat ggtcttgccg atatcttgtt tctctaagtc
     3301 ttttttaccc aaggatgcac ctcgttaaaa agtggtttct acaaccctct gcccctgcgg
     3361 tttttcaatt ttgtcaggga cgataccggc catcaacaac cctaaagttt ctttggctac
     3421 ttcctcggct tcaaggaggg cgataatttt gccacggtac atcaccgcga tgcggtccga
     3481 aagttcaatc acttcatcga gttcaggtga aaccagcagc accgcgcagc cttcatctct
     3541 tttctgtacg atgcgcttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct
     3661 cggtctgacc attcccctgc accccggcga tgcccagaat ctgaccggcg tgaacctcaa
     3721 aggatacttt atccaccatc acctgtttga actggtcagt caccaccagg tcttttactg
     3781 aaagtatcac atcaccaggt tttttgatat ctttttccac caccaggttg acttcgcgcc
     3841 caaccatcat ttctgccaat ttgttgcggt tcgcttgttt ggggtccgcc tctcccacca
     3901 ccgcgccgcg gcggatgacc atgatacggt ctgccacgtc taaaacttcg cgtaatttat
     3961 gggtgataaa gatgatggac ttgccctgtt ttaccaggga acgcatgatg acaaagaggt
     4021 cgtccgcttc ctggggggtc agcacagcgg tgggctcgtc aaagatgagg atattggctt
     4081 cgcggtacag gagcttgatg atttctacgc gctgttgcac gccgacgggc aggtctttga
     4141 cgtaatcatt agggtcaacc tccagtttgt attgactgga gatttccata atcttcgtgg
     4201 ctgtcttggg ccggtcaagg aaatcgccgg ctttcactgg ctcagaaccc aacatcacgt
     4261 tttcagttac agtgaacacc ggtatgagca taaagtgctg gtgaaccata ccgataccag
     4321 cattgatggc atcagttggc gaatgaatgg ttatcttttc gccattcacc aaaatctcgc
     4381 cttcatctgg ctgatacaac ccgtagagaa tgttcatcaa ggtggtttta ccagcgccgt
     4441 tttcacccag caacgccaat atctcgccct gattcaaagt gaggtcgatg tggtcatttg
     4501 ccagaacgcc gggaaactgt tttgtaattc ctttgagttg aagaatcgga gccatgtata
     4561 caccacttgt tcagagggga ttttgattat tgtatcacag gcgtctcaaa ctcaaaaatt
     4621 aaaaaaaaag ctggagcgaa taactccagc tttttctcac atgtgcgcga attactgctt
     4681 gaaagcgggg ttcatctgga tttcgccgga gaggatgcca gctttaatcc cttcgagttc
     4741 ggctttcagc tcagcggaaa cctgaccgtc gaagttatgg aacggtgcga tacctacacc
     4801 accattggcc agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccatat
     4921 cgctgccttt ctgctggtcg ctgaagtcat tggtgaagag cccggtctgt gcgtctgcat
     4981 cccagccgag ggtctctact gaagtaccgt gcacttcatt gtagtggcgg acgccgcgcg
     5041 cgaagccatc catgaagatg gtcacagttg gaatctgcat tccaccgaag gtgccgacct
     5101 taccggtctt ggtgaaacca gccgcggcgt aaccagccag gtaagcggct tcatcagtct
     5161 ggaaagcctg ggccaagaca ttggggatta cgggatcata gccataatca acaatggaga
     5221 acttctgatt gggattggcc tcagccgctg ccttggtggc atcgcccagt aggaaaccaa
     5281 cggtgatgat gatgtcgcat ttctcagcga tgaaggcgtt gatgttcttc tcgaaatcag
     5341 cgatttcttt tgattccaaa tacttggctt caatatccag cttttctttg gcatctaccg
     5401 cgcccttcca tgcggtcgcg ttgaaggatt tgtcatccac gccaccggta tcggtgactt
     5461 cgcaggcttt aaattttgcc ggtccggctg ttttcgcgca cgcgctcaac acaaaaacgg
     5521 caattagaag cactgacatg acgagcatta cttttttaga cattttttct cctcctgcag
     5581 ggttgttgga tgtgcggggg actgccccgt ctattaattg agtataccga tatttgccct
     5641 tcaatgcaag ttatcccagc catgaaaaat cccagatgga gagacttttt cattcaggac
     5701 cgtttattcg ctggcggatg aggatgcgcc aatacccgcg cccagcttca ttttttcttc
     5761 gtgcgcgcgg tcttttacct tcagcatgga taagaacgcc aggaacagaa agaccgcgcc
     5821 aaagataaag agcactttgt agttgaagtg tattctttca accagcagcc cgcccagggt
     5881 tggtccgagc acggctgcgg tttgtgagaa gaaataataa agaccggtat acgcgccaat
     5941 gcgtttttca tctccatggt cgtacaccag cgggaggctg ttgacgttga cccaggacca
     6001 gaagaaccct accagcacca ggatgataat gaaggagatg agccctttca ccaggaagaa
     6061 actgatgaga aagatgagcg tgagcgcgaa cacgccatag aggatggtcc tcttacgacc
     6121 caacctgcca gcgagaattc ctgaaggaat gcagaaagcc aggaaagaaa gattggcaat
     6181 tgaagccacg atcgaagcct tgccaggggc aatgcccaga tcgaagaccg cgaaggaaga
     6241 caaccccgtt tcaatggcgt taaagccggt gaaccagcag aagatggcca gtagaatgaa
     6301 aagcgttgat ttatcctggt tgctgaagac cgctttaagg ttgggcacca atcccttcac
     6361 cgcgccgctg ctgtcctcat cttcggtgtg ctttcccagg cgttctttgg gttctttcac
     6421 ccagatcaac accgcggcca acgccacggt catggcgacc gcgccggcga cgaagggacc
     6481 catcctgcca aaggcgttga agatcagccc tcccagtacg tacgaaagga caccggcagc
     6541 gccgcccatc aggttgatga cgccgttcgc cttgctgcgc tgctctggca ggaacaggtc
     6601 gcccagccat gaaacaaccg gagagcggaa aagtgccatg ccgacattgg tgaccatgat
     6661 gaacaccgcc accgccagca ctgattgcgc caacggaatt gaaataaagc ccacgagcgc
     6721 gatgggtaaa cccagtagaa tccacggttt tcgccgaccg aaacgattcc aggtgtgatc
     6781 gctgcgattc cccacccatg gctgtacgaa gatgttgacg atattgtccc aggtcatgat
     6841 gaacatggcg agcgctgggc caatgccaaa accgacaaaa ttggggatgt tggttttaaa
     6901 tccgggatta cccgcctgga ggatcagcgg cacccattga ttgaagattg gccataaaat
     6961 actgatgcca aaaaaaccaa accctaccag aaacgtacga ccccatggaa acttcttttt
     7021 ctccatctct tactcctttg tttttgtggt agattgaacc ttggattcac catttaacag
     7081 gtctggacgg tgcttttggg tgcgcaacag ggattgttct cggcgccatt tggcgaccag
     7141 ggcgtgattg ccagagataa gcacttctgg tacgcgccaa ccacggaatt cttctgggcg
     7201 ggtgtagtgc gggtactcca ggagaccatt gccaaaacta tcttcctcgg ctccgtcagg
     7261 gtcacccagc acgccgggaa taaagcggga taccgcgtcg atgagcacca acgctggcag
     7321 ctcgccaccg gtgagcacat aatccccaat cgagatctgt tcagtcacca gatgctcaat
     7381 cacgcgctcg tcaacccctt cgtatcgtcc gcacaataac cccagtcgag gttgttgcgc
     7441 cagctcgcgc gcgatggcgg tagtgaaagg ttttccctgc ggagagaggt agagcagcgg
     7501 gcaggatgga ggggctccca agacggactc gacagcagcg aagataggtt ctactttcat
     7561 caccatacca ccgccgccac catagggggc atcatcggtc acatggtgcc ggtcaaaggt
     7621 ccagtcgcgg atgttgtgca gcgcgacttt aatgaggtca ttttgttggg cgcgctgcag
     7681 gatgcttgaa tcgaggtaag gccgcagaac ccctggaaag agagtaaaga catcgaattg
     7741 catggcttca ttctagcaaa gaagcgcgaa cacaggcaag ccgttttcct catgaaaaat
     7801 ggcgcatcag gaggagagaa ataagttcga tggtgagcgg accggtagga agccttgctg
     7861 tttatccccg tatgggagcc aagtggaaag gagaagtgaa gccctgccca cgctaaccat
     7921 gaaggttaac cgtgattgca cagagaggcg agggagggct tccggtcatc ttgacgctgc
     7981 tggtttcact tggtaagatg aattccatgt atatcaaggg cagcaagtgg agcatgcaga
     8041 aaaagaaacg caggtcgaac ccattccgcg tgatcctgtt gatcgtattg atcgccgcgg
     8101 ccatctactt tgaccgcatg attgtgccta ctatcccgca gttttttctg cctacgccca
     8161 cgcctacccg cgcgcccgaa tccatcacct cagacgcgaa agggttggag gctgaaggga
     8221 aataccctct ggccatcgta gcttataaag acgcggtcag agctgacccg cgcaacccgc
     8281 ataacttcat tgcactggcg cgcttgaaca tttataccgg taattatgaa gaagccgtca
     8341 ccgatgcgga aaatgcctta ttgcttaacc cgaacaacga cgaggcgatg gcgctgcgcg
     8401 gctgggcgat tgggttgacg ggagactatg tgcgcggcac gggcatcctg caagaggcta
     8461 ttgctgcgaa ccctgattac gccgcggcgt atgcctacct ggcggagatt tacacatata
     8521 tgctgcaggc aggacaggga gacctgcaga ccctggacaa tgccattaat gcctcgcgcc
     8581 gcgcggagga actggcgccg ggcaccctgg aaacacaccg tggtcgtgga atggtgcttg
     8641 aatacacctc gaatttcaaa gaagcagtgg cggaatttga ggcggcggtg gctttgaatc
     8701 cgaacatcgc tgacctgcac cttggcctgg ggcgcaatta tttcaacatc caggaatatg
     8761 acgatgccat cactgaattt ggccgggcaa acgctttgaa ccccagggat ccgacccctg
     8821 aatctttaat cgctcgaact tacgctttga atggcgatta ccagaaagct attcaatacg
     8881 gcgaggcggc gattatggat gagccacaga atgcgcgact gtatggcaac ctggggctga
     8941 tctacagcag cgcgaagcag taccttgacg cgatcgatat gctgcgaatc gcggtgcgtg
     9001 gcggggtatc ttccgatggc gctccggttg agggcatccc aatggattat ggccgggtgg
     9061 cgcagtatta ttacacttat gggctcgccc tggcgagggt ggggcaatgc ggcgaagcgc
     9121 tggaaatttc tcaggcggtt cagatggggc tgcgcaatga tgacatcgct gttttcaatg
     9181 ctgaagaaat ggtcaatatc tgcgagcgcc tcgcccgtga aggtaaatct gatatcgcgg
     9241 caacggccac acccgctgag acaaccccaa cagtggaagc cgcgccctga ccattgggta
     9301 tgacgggcaa tcgcaactat gatcgactat agcggaaaac gcgctttatt cggccatcca
     9361 agaaaaagat ccaacccaat ttggatattc accctgctgt tgttgatact ggcgggcctg
     9421 tttgtgattc aggcagtgcg tgatggccgc atcgctccca tattacaaat cataccaacg
     9481 ccaactcgca gcctgaattc ttacgtgcaa gaaggggaaa ctcattttca ggcggggaac
     9541 ctggagaaag cgatcgaggc ttaccgctac gccattgcca tcgaccctga agacgccgac
     9601 ctgagggccg agctggcgcg catccaggtt tactcaactt cgcaaatcac ggttgatgcg
     9661 cgcaaaagag agcgcctgga agaggcgctg gcgaccattg aagaagggtt ggcaatcagc
     9721 ccgcaaaaca gtaacctgta cgcggttaaa gcctttgcgt tagattggta cggctcttca
     9781 ggggcagcag gagaagcctc gcaggagtac ctggtcaaag gggaacaagc cgccacagaa
     9841 gcgctgcaat atgataatac caacgcgttg gcgctggctt attacgcaga attattgatt
     9901 gatcagttga agtattcaca agcgcaacag tacatcaccc aggcgctgga gcgggatgac
     9961 acccttatgg atgtgcaccg ggtgaacggc aatgtgcagg aagccagcgc gaactatact
    10021 caggcgatcg aagagtataa gaaagccatc caaattaccc cgaacctgaa cttcctgtat
    10081 ttgtcattgg gcgcgaatta ccgccaactg gccaacatga cgggaatcac aaccgaacag
    10141 aactactatt ttgggctggc gttggaagct tttgccaagg ctgtggcgat caacgaccag
    10201 ttaggcgtca gggatcccat tccgctcatc tccattgcca atacatatgt ccagcagggc
    10261 gaatttttcg ccgcggcgcg taacatgctg cgcgcggtgg catttgctcc tgatgacccc
    10321 accgcgtacg gtcagctggg tgt
//
LOCUS       DAOSPM010000064        10227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_6549_length_10227_cov_17.597523, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000064 DAOSPM010000000
VERSION     DAOSPM010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6549_length_10227_cov_17.597523"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      518..652
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            716..1549
                     /locus_tag="PLW25_06555"
     CDS             716..1549
                     /locus_tag="PLW25_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS54183.1"
                     /translation="MRTNDDLKTPMGDTLGGSQPALEFLARHNGWLAGISLLVALGVW
                     ALIIDWGKPPVFLLPPPGAVWNKFLQVAGDGTLLFHAGVTLSEVLLGLLAGSLVAMAL
                     GYLLAKSRLLEKIISPYLVASQAIPTVAIAPLLIIWFGQGIFSKVLICALTLFFPVLV
                     NTIIGFRAVPANLYDLMRSMRATHAQTLRYLELPAALPVLFGGLRIGAALSVIGAVVG
                     EFVGADRGLGFLINVGRGQFDTALVFVSLFTLILMAMSLYGLAMLAERRATRWRVADR
                     G"
     gene            1572..2582
                     /locus_tag="PLW25_06560"
     CDS             1572..2582
                     /locus_tag="PLW25_06560"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS54184.1"
                     /translation="MKKTSQTFFLLLFIGVILIALFAVTRVIAPAVEPTSTSAPLRKV
                     RLPVGYIPNVQFAPIYVAIEKGYFRDAGLDVAIDYSMENDNTVLTATGDLQFAVVSGE
                     QVLLARAQQMPVVYVMAWYQQYPVGIAAKKSAKINTVEDLRGRKIGLPGLYGASYIGA
                     IALLDSAGLKEADVKLDSIGFTQVESLIADRDEAVVIYVANEPVQLERLGEEITLLRV
                     ADAVDLVANGIITNEMTLQKDPELVRSMVTGMIKGIQYTIDHPEEAFEISKKYVENLA
                     SADQEMQMQVLLNSIALWRDDRLGYSQPQAWENMQRILLKMGLLKDELDLSKVYDNQF
                     IP"
     gene            2592..>3214
                     /locus_tag="PLW25_06565"
     CDS             2592..>3214
                     /locus_tag="PLW25_06565"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS54185.1"
                     /translation="MSNGFLEVRQLGMTFRGAEGGLEALREISFDLGSEEFLCVLGPS
                     GSGKSTLLRLIAGLLKPTSGWIRFGGADGAPRVGLVFQHANLMPWRTVRQNIALPLEL
                     LGMAEEESASRVAEMMRLVGLEEFGSNWPSELSGGMAQRVALARAFIQEPDLLLLDEP
                     FGALDALTRERMGSELLRIWQGQRVPVVMVTHSISEALLLSDRVLLFT"
     assembly_gap    3215..3314
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3315..4126)
                     /locus_tag="PLW25_06570"
     CDS             complement(<3315..4126)
                     /locus_tag="PLW25_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOS54186.1"
                     /translation="MSWNLLGHTWAENLLRTHIANNEARHAYLFTGAPGVGRRSLALA
                     FASALNCTQPQQPGDYCENCRICRHTQRMQLPDLSVVAPESEGGSIKVAQVRELQRAL
                     SLTPYEARYRVALLLNFQLANASAQNALLKTLEEAPEKVILLLTADAADSLLPTIVSR
                     CEVLRLRPLAVEALEDALLNRWQLAPQQARLLAHLSSGRPGMALQMAQDQHLLEKRAA
                     WVQELLRLLPANRYERFSACEALARNRDQLRLMLQVWLSFARDLLLTASGSS"
     gene            complement(4175..5887)
                     /locus_tag="PLW25_06575"
     CDS             complement(4175..5887)
                     /locus_tag="PLW25_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOS54187.1"
                     /translation="MLIYAQRQDPFETRLLKLHRDAAELVGNLSYDEVLEKIASIALE
                     RSGAGYAAVATLDEHGEIERFVPAGMLRTQIEKIPHPPQGFGLIRAMMNTRSAMRIPA
                     ISKDQRHSGFPPEHPKMSSFLGVPIFQGQRQIGQIYMTDKVITGEFSEEDQRLMELLA
                     AYAGVAIVNARLFRDYAMRDRALTRQNENLSLLNQLASTLSTSTEVNEILEQGMMQLM
                     DYLRLEVGEIFIKNEDSRRLNLLIHHGEAVDSLWKRAQFSIGESTVGRAAKGDVPIVL
                     NLTEHEYADLNPLTKARGLHQMAVIPMKGRRGVVGVLCVATCHPQPLNEPDVQFAQAI
                     AAWMATAIENIRLNITGRRLAILEERDRIGMDLHDGIIQSIYAVGLTLEHARLLMKDN
                     PDTATSRIQQAVNDLNSTIRDIRAYILDLRPRQLKNENLMEGINRLVQEFRANTLVDV
                     TLNGPKDGVATLPEAQALALFHICQEALANIAKHAKAKRAYVSVWTTSERVLLEVHDD
                     GLGFEQNKTKLSIGHGLSNMETRAINAGGEVDISSEPGGGTTVLAWVPFPDPDFSSND
                     RVIE"
     gene            6024..6662
                     /locus_tag="PLW25_06580"
     CDS             6024..6662
                     /locus_tag="PLW25_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HOS54188.1"
                     /translation="MDSIFRRKTEEEQMKAAGRMPPGQFLTQKFPVLHYGAVPGFDPV
                     NWDFRVWGEVEQTLRLTWAEFSQLPRTTLIMDIHCVTRWSKFDTLWEGVSIRTLIENR
                     LIRLKPQARFVIQHAEYGFSANLPIEIALADNFLLATHYDGAPLTPDHGYPLRGVVGA
                     IPQRPDLKTPYFWKGAKWLRGLEFSAVDRLGFWEEAGYHNEADIWKEQRIAE"
     gene            complement(6715..>7188)
                     /locus_tag="PLW25_06585"
     CDS             complement(6715..>7188)
                     /locus_tag="PLW25_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54189.1"
                     /translation="PTSILPTPEPVKGFGTLCIYLYNDINGNAMAEEEETMNTGIAGG
                     QVSITHLGGEYSNVGTTLSTGEALCFNDIPEGEYSISIAIPDGYNPTSSQNVTVLLKA
                     GDTSTVNFSAQVNGLAESPRGNGDAGGSVLLAVIGGLILLAGVGVGLYARYILRK"
     assembly_gap    7189..7288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7544..7957
                     /gene="ssb"
                     /locus_tag="PLW25_06590"
     CDS             7544..7957
                     /gene="ssb"
                     /locus_tag="PLW25_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOS54190.1"
                     /translation="MYQKLVIVGRLGRDPEMRYTPTGQAVTSFSVATDRQYSDQSGKP
                     VKETVWFKVTAWGKLAETCNTYLQKGKLVLIEGRLTVDTKTGGPRVWTGQDGQARASY
                     EVTAQTVKFLSQRQEGGEPGQEEELDIAPSEEIPF"
     gene            7967..8302
                     /locus_tag="PLW25_06595"
     CDS             7967..8302
                     /locus_tag="PLW25_06595"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOS54191.1"
                     /translation="MSNRPQNPPLGLPPMELPDELEPVYSNLARISHTPSELAVDFSR
                     ILPGQKRLTVLVRVLLSPVGAKLLLRALAENIARYEAAFGEIYLPGDSGLAEDLFRSV
                     HPPEPPKSG"
     gene            8306..8947
                     /locus_tag="PLW25_06600"
     CDS             8306..8947
                     /locus_tag="PLW25_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HOS54192.1"
                     /translation="MNEFNEIVESTRKVFDERTKVRDAALVQARQLTRVSALTIRAIH
                     RGDKDEAHGLLNEARQLVLTLQRELADYPDLYHAGYTQDAIKEFAEASLTCALIENRR
                     LPRPEELEIEHATYVNGMAEAIGELRRRCLDILRQGYSVEAERLLNTMDEIYNFLVTL
                     DYPDAITNGLRRQTDLVRGIIERTRADLTLSLREQHLQEALQRFSEKYPGLAE"
     gene            9125..10024
                     /locus_tag="PLW25_06605"
     CDS             9125..10024
                     /locus_tag="PLW25_06605"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOS54193.1"
                     /translation="MRPIKLRYNKETMSTPSVHRIAIVVRPGSTDTLSEGAVIQQWLK
                     SHGVSSTVTTTDNQHLRRKISQGEYDLLIALGGDGTMLRAGHLAAPQGLPILGINLGK
                     FGFLMQLSREDWADTLPRLLAGDYKVENRMMLQAQQWRGDERLRTSQVINEVVVARGQ
                     VVRPIRIHAEVDGYPLASYVADGLIASTPTGSTAYALAVGGPIMPPELRNILIMPVAP
                     HLSMDRAVILPEGASVVITVDSDHEAVMSVDGHEPALISNQDRVTVTASDLSVKFVVF
                     QEPGYFYRNLSNYMEQNPSIGDF"
     gene            10024..>10227
                     /locus_tag="PLW25_06610"
     CDS             10024..>10227
                     /locus_tag="PLW25_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54194.1"
                     /translation="MAEEATSESMHCYVHPQRETLLRCNRCEKPICLSCAVLTPTGYR
                     CKECVKGQQRIFETARWWDYPLTF"
BASE COUNT         2196 a         2661 c         2987 g         2183 t
ORIGIN      
        1 gttcaaggcg aactgaatat tactggttca acagcaaggg agacgccatg tgggtagccg
       61 cctacaacgg cctgattacc ggttatgcgg gcatcagctt catgccgaaa aactaagcct
      121 ggcttcttta acaaacgaac tcccgtgtcc aattggacac gggagtttga ttatcaatag
      181 aactcatcct gaagacgagc ctaattgcaa atggagcaag ccggagtttt ccgctcctaa
      241 cgggattggg cgaacatatc cgtacagcct tagagggaga aattcgttat atagatagaa
      301 ccaaccctca agcaagcgaa tcctttcaga tcagtgctgg gtatcttgaa ctgaaagcgc
      361 aggttcgttc actggttcac ctcaatccgt attttcattt aggcatacat ttaagagata
      421 aggaaatatt atcggaacag ggcgaggtca taccggttca cccctcagct ctgcttgaca
      481 tcacccctgc accggtataa aatacgctca attcaatacg cttcttcggg gcagggtgag
      541 aggttcgcgc ctcaattccc gatcggtggt gataacccac gagcgcatta cacgcgcagg
      601 atccggtgaa attccggggt cgacggtaca gtccggatga aagaagagca gccagaacag
      661 gcagaatacc cgcctgtcag ccagccccga aaaccaacgc gtttcgaggc tttttttgcg
      721 cacgaatgat gacttgaaaa ccccaatggg tgatacgctc ggcggcagcc aaccggcgct
      781 ggaattcctg gcgcgtcata acggttggct ggcaggaata tcgctgctgg tagcactggg
      841 agtatgggcg ctcattattg actggggcaa gccgccggtt tttttactcc ccccgccagg
      901 cgcggtatgg aacaagtttc tgcaggtggc gggtgatggc acgctgctct ttcacgcggg
      961 ggtaacgctc tccgaagtgc tgttgggtct gctggcgggc agcctggtgg cgatggcgtt
     1021 gggttatcta ctggcgaaat cccgcctgct ggaaaagatc atttctccct acctggtggc
     1081 cagccaggcc atccccactg tggctattgc gccgctactt atcatctggt ttgggcaggg
     1141 cattttttca aaggtgctta tttgcgcgct caccttgttc ttcccggtgc tggtgaacac
     1201 gatcatcggc ttcagggcag tacctgccaa cctgtacgat ctaatgcgtt caatgcgcgc
     1261 cacacacgcg caaacgttgc gctacctgga actgccagcg gcgctgccgg tgctctttgg
     1321 cggcctgcgc attggggcgg cactttcggt gattggcgcg gtggtgggag aattcgtcgg
     1381 cgctgaccgt gggttgggtt ttctcatcaa cgtgggacga gggcagtttg ataccgcgct
     1441 ggtgttcgtt tctctgttca cactgatttt gatggcgatg agcctgtacg ggttggcgat
     1501 gctcgccgaa aggcgcgcca cgcgttggcg ggtggctgat aggggttaaa atctcgttcg
     1561 cgagggaaca catgaaaaag acatcacaaa cattttttct tctcctcttc atcggagtca
     1621 ttctcatcgc gttgttcgcg gtcacacgcg tcatcgcgcc cgcggtggaa ccaaccagca
     1681 cctccgcgcc actgcggaag gtgcgcctgc cggtgggata catccccaat gtgcagttcg
     1741 cgcctatcta cgtggcaatt gagaagggct actttcggga tgccggcctg gatgttgcca
     1801 ttgattacag catggagaat gacaataccg tgcttaccgc caccggtgac ctgcagttcg
     1861 cggtagtctc aggtgagcag gttctgctgg cgcgcgcgca acagatgcca gtggtgtacg
     1921 tgatggcctg gtaccagcaa tacccggtag gcatcgcggc gaaaaaatcc gccaagatca
     1981 acacagtgga agacctgcgc gggcgcaaga ttggcctgcc cgggctctac ggcgcttcgt
     2041 acatcggcgc catcgccctg ctggactccg cggggttgaa agaggcggat gtgaaactgg
     2101 attccattgg cttcacacag gtcgaatcgc tgatcgctga ccgagacgag gcggtggtga
     2161 tttatgtggc caacgagccg gtgcagttag agcgcctggg tgaagagatc acattactgc
     2221 gggtcgcgga cgcggttgac ctggtggcga atggcattat caccaatgag atgacattgc
     2281 aaaaagaccc tgaactggtt cgttcgatgg taacgggaat gataaaaggc attcaataca
     2341 cgatcgacca tcccgaagag gcgtttgaga tcagcaaaaa atacgtggag aatttagcct
     2401 cggcggacca ggaaatgcaa atgcaggtgc ttctcaactc cattgcttta tggcgagacg
     2461 atcgcctggg ttactcgcag ccgcaagcct gggaaaatat gcagcgcatc ttgctgaaaa
     2521 tggggctgct gaaagatgag cttgacctga gcaaggttta cgacaaccag ttcatcccat
     2581 agaagataag aatgagcaac ggtttcctcg aagtcaggca gctgggcatg accttccgcg
     2641 gcgcggaagg cgggcttgag gcgctgcgcg agattagttt tgatcttggt agtgaagagt
     2701 tcctgtgcgt gctgggtcct tcggggagcg gcaagagcac gctgctgcgg ctcatcgccg
     2761 ggctgctcaa gccaacatcc ggctggatac gctttggtgg cgcggatggc gcgccgcggg
     2821 tggggttggt ttttcagcac gccaacctta tgccctggcg cacggtgcgg cagaatatcg
     2881 cactgccatt ggaactactg gggatggcag aagaggagag cgccagcagg gtagcggaga
     2941 tgatgcgcct ggtggggttg gaagagttcg gcagcaactg gccttccgag ctttcgggcg
     3001 gcatggcaca gcgcgtggcg ctggctcgcg cctttatcca ggagcctgac ttgttgttat
     3061 tggatgaacc tttcggcgcg ctggacgcgc tgacgcgcga acgcatgggc agcgaattac
     3121 tgcgcatctg gcaagggcag cgtgtgccgg tggtgatggt gacgcattca atttcagaag
     3181 cgctgctgct ctctgaccgt gtgctgctct tcacnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnntcactg ctgccgctgg cggtgagcag caggtcgcgg gcgaacgaaa
     3361 gccaaacctg cagcatcagg cgcaactggt cgcggttgcg ggccaacgct tcacaggcgc
     3421 tgaatcgctc atagcggttc gctggcagca ggcgtaacag ttcctgcacc caggcggcgc
     3481 gtttctccag caggtgctga tcttgcgcca tctgcagtgc catgccgggg cgcccgctgg
     3541 ataggtgggc aagcaggcgg gcttgctgcg gcgccaactg ccagcggttg aggagcgcgt
     3601 cttcaagagc ttcaacagcc agggggcgca gccggagaac ctcacaacgg gagacgatgg
     3661 taggcagcag cgaatcagca gcatcggcgg tgagcaacag gataaccttc tcaggggctt
     3721 cttcgagtgt ctttaaaagc gcgttctgcg cgctggcatt cgccagctga aagtttaaca
     3781 atagcgccac acggtatctg gcttcgtaag gggtcagtga aagggcgcgc tgcagctcgc
     3841 gcacctgtgc cactttgatg ctgcccccct cgctttcagg tgccacgacg cttaagtcgg
     3901 gcaactgcat acgttgagtg tgcctgcata tacggcaatt ctcgcagtaa tcgccgggtt
     3961 gctgtggttg ggtgcagttg agggcgctgg caaaagccag ggccaggctg cgacgcccca
     4021 caccgggagc gccggtgaag aggtaagcgt ggcgcgcttc attattcgcg atgtgtgttc
     4081 gcagcagatt ttcagcccag gtatggccca gcaggttcca gctcatagga ggattgtagc
     4141 ctaaaggaac gccactggca accgtaaggg cggtttactc gatcacgcgg tcgttgctgg
     4201 agaaatccgg gtcagggaag ggtacccagg caagtacggt ggtgccgccg ccaggttctg
     4261 aggagatgtc tacctcacct cccgcgttga tggcgcgtgt ttccatgttc gaaagtccgt
     4321 gcccgatgga gagtttggtt ttattctgct caaaaccaag cccgtcatcg tgaacctcca
     4381 acaacacccg ttcggaggtg gtccacacac tcacgtatgc gcgtttggct ttggcgtgtt
     4441 tggcgatatt ggccagggcc tcctggcaga tgtgaaagag ggccagcgcc tgggcctcag
     4501 gcagggtggc gaccccgtct ttgggaccgt tgagcgtgac atccaccagt gtgttggcgc
     4561 ggaattcttg caccagccgg ttgatgccct ccatcaggtt ttcgttctta agctggcgcg
     4621 ggcgcaggtc gaggatgtat gcgcggatat cacggatggt gctgttaaga tcgttgaccg
     4681 cctgttggat acggctggtg gctgtatccg ggttgtcttt cattaacagc cgggcgtgct
     4741 caagcgtgag ccccacggcg tagattgact gtatgatgcc gtcatgcagg tccataccga
     4801 tgcggtcgcg ttcttcaagg atagcgaggc ggcgcccggt gatgttcagg cggatgtttt
     4861 cgatggcggt tgccatccag gcggcgatgg cctgggcgaa ttgcacatca ggctcgttca
     4921 gcggctgcgg gtgacaggtc gccacgcaca aaaccccaac aacgccgcgc cgccccttca
     4981 tgggaatgac cgccatctgg tgcaacccgc gcgccttggt gagagggttg aggtcggcgt
     5041 attcgtgttc agtgaggttg agaacgatgg gcacatcgcc cttggctgcc cgccctactg
     5101 tactctcgcc aatagagaat tgcgcgcgct tccacaggct atccaccgct tcaccatgat
     5161 ggatgagcag gttgaggcgc ctgctatcct cgttcttaat gaaaatctca cccacttcca
     5221 agcgcaggta atccatcaac tgcatcatcc cctgttcgag gatttcgttc acttctgtgc
     5281 tggtggaaag ggtgctggca agttggttga gcagagaaag gttttcgttc tgccgggtca
     5341 acgcacggtc gcgcatggcg taatcacgga acaggcgcgc gttgacgatg gcgaccccgg
     5401 cgtaggcggc cagcagttcc atcaggcgct ggtcttcttc tgagaactca cccgtaatca
     5461 ctttatcggt catatagatc tgaccgatct ggcgctgccc ctggaagatg ggcacgccga
     5521 gaaacgaaga catcttggga tgctcaggcg ggaagccgct gtggcgttga tctttactga
     5581 tagcggggat gcgcatggcg ctgcgcgtgt tcatcatcgc cctgatcaag ccaaacccct
     5641 gcggcggatg agggattttc tcaatctggg tgcgcagcat ccccgcggga acaaaacgct
     5701 cgatctcgcc atgttcatcg agggtggcaa ccgcggcgta acctgcgccg gaacgttcca
     5761 gggcgatgct ggcaattttt tccagcacct catcatatga aaggttgcct accagttcgg
     5821 cggcatcccg gtgaagtttt aacaaccggg tctcaaatgg gtcctgtctt tgcgcgtata
     5881 tcagcatttg ctgtcctgtg ggctcaattg taaaccaggc gcaggatata tgtcaatttt
     5941 tgtccaaatt tgagtttatc caaatttgtc tcaaacagct tctgggtaag gcaaaaaaca
     6001 gatataatga cctcaggtga aatatggaca gtatttttcg acgcaagact gaagaagagc
     6061 aaatgaaagc ggcgggacgt atgccgcccg ggcaatttct cacccagaag ttcccggtat
     6121 tacattacgg cgcggtgccg ggttttgacc cggtaaactg ggacttccgc gtgtggggcg
     6181 aggtggagca aacgctgcgc ctgacctggg cggaattttc acaactgccg cgaaccacgt
     6241 taatcatgga tattcactgc gtgacgcgct ggagtaagtt tgatacgctc tgggaaggag
     6301 tatccattcg cacgctgatt gaaaacagac tgattcggtt aaagccgcag gcgcgctttg
     6361 ttattcaaca cgctgaatac ggcttcagcg ccaatcttcc tatagaaata gcgctggcag
     6421 ataacttcct ccttgccacg cattatgatg gcgcccctct cacccctgac catggttacc
     6481 cgctgcgcgg tgtggtgggc gctattccgc agcgcccaga cctgaaaaca ccctactttt
     6541 ggaaaggcgc caaatggctg cgcggcctgg agttttcagc ggtggatagg ctgggtttct
     6601 gggaagaagc cggttaccat aacgaggcgg atatctggaa agagcagcgc atagctgaat
     6661 agaccccgct gacaaccggg aagggggcaa aggcggttta caaggtgatg ttgactattt
     6721 gcggaggata taccgcgcgt aaaggcccac gcctactccg gccagcagga tcaacccgcc
     6781 aataaccgca agcagaacag acccgcccgc atctccgttt cctcgcgggc tttccgccag
     6841 gccgttgacc tgcgcgctga aattaacggt ggaggtgtcg ccggctttca gcagcacggt
     6901 cacattctga ctggaggtgg gattatagcc atcagggatg gcgatactga tagagtactc
     6961 gccctcaggg atatcgttga aacacagggc ttctcccgtg cttaaagttg tgcctacatt
     7021 ggagtattca cccccgaggt gagtgatgct gacctgcccg ccggcaatac cggtgttcat
     7081 ggtttcttcc tcttcagcca tggcgttacc gttaatatcg ttataaaggt aaatgcacag
     7141 cgtgccaaag cctttgaccg gttctggtgt gggcaggatg gaagtaggnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cgaagtgatt accagaacag ccgccgccag
     7321 gaggatgaac aagaaaatca ggatgcttcg aggttttatg gcattcactg gcaagattat
     7381 aacattattc cctggctggt ttttcgaccc tgcgccgaac cgggcggcgc gcggctgagc
     7441 tgtcaataat atctcccttc tggcaggatt gaattaatga tttttattgt aaaatgaata
     7501 ataaccgcac acgattttta cacggtacct taaggaggaa caaatgtatc agaaactggt
     7561 gattgttggc agattgggca gggatcctga aatgcgctat acccctacag ggcaggcagt
     7621 aacaagtttt tcggtggcga cagaccgtca atactctgac cagagcggca aaccggtgaa
     7681 agaaacggtg tggttcaagg tgaccgcctg gggcaagctg gcggagactt gcaacactta
     7741 tctacaaaaa ggcaaactgg tactgatcga aggaagattg acagtggata cgaaaacagg
     7801 cggcccgcgg gtttggacgg ggcaggatgg ccaggcgcgc gcgtcatacg aagtcaccgc
     7861 ccagacggtc aaattcctgt cacagcgtca agaaggcggc gaacccgggc aggaagaaga
     7921 gctggatatc gctccctctg aagagatacc gttttagcga gctcccatga gcaacagacc
     7981 gcaaaatccg ccccttgggt tacccccaat ggagttgccg gatgaattag agccggttta
     8041 cagcaacctg gcgcgcatca gccatacccc ctcagaactg gcggtggatt tctcccgaat
     8101 cctgcctgga caaaaaaggc tcaccgttct ggtgcgggtg ttgctctctc cggtaggcgc
     8161 caagctgctg ttacgcgcac tggcagagaa cattgcccga tacgaggcgg ccttcggtga
     8221 aatctatctc cctggcgatt cagggctggc agaagacctt ttcaggtcgg tgcaccctcc
     8281 cgaaccgcca aaatcgggtt agcccatgaa cgaattcaac gaaattgtgg aaagcacgcg
     8341 caaggtcttt gatgaacgca ccaaagtgcg tgacgcggcg ctggtacagg cacgccaact
     8401 cacacgggtt tctgctctta ccattcgcgc catacaccgc ggtgataaag acgaagcgca
     8461 tgggctgctc aatgaagccc gccaattggt gctcacctta cagcgtgaac tggcggatta
     8521 ccctgacctc tatcacgccg ggtacaccca ggacgcgatc aaggaatttg ctgaagccag
     8581 ccttacctgc gcgttgatcg agaatcgccg gttgccgcga ccggaagaac tggaaatcga
     8641 acacgctact tatgtcaatg ggatggcgga agcgatcggt gaactgcgcc gcagatgcct
     8701 ggatattctg cgccagggat actcggtaga agccgaacgg ctgttaaaca ccatggacga
     8761 gatctacaac tttttagtga cgctggacta ccccgacgcc atcaccaatg ggctgcggcg
     8821 tcagaccgac ctggtgcgcg gcattatcga gcgcacgcgc gccgatctga cgctgagcct
     8881 gcgcgagcag catttgcagg aagcgctgca gagattctct gaaaagtatc ccggattggc
     8941 agagtagcac caggcgttca tggggaaatt cttcacttct gaaaagaaac ataaaagacc
     9001 acacaggtta tctgcgtagc cgttacttta cattgtcggc tgttaaatct cgtgatgatg
     9061 gcgcgacata tgaacaccac agccagacgc gtcatgaccc cgttacggtt ggtgatgagt
     9121 attcatgcgc ccgattaaac ttcgttataa taaagaaacc atgtctacgc catctgttca
     9181 ccgtattgcg atcgtggtgc ggccggggtc gaccgatacc ctgtcagaag gcgcggttat
     9241 tcagcagtgg ctgaagtcgc acggcgtaag cagtactgtc accaccactg acaaccagca
     9301 cctgcgcagg aagatcagcc agggggaata tgacctgctc attgccctgg gcggagatgg
     9361 caccatgctg cgcgccggtc acctcgcggc tccgcaggga ctgcccatat tggggatcaa
     9421 cctggggaag ttcgggtttc ttatgcagct ttcgcgagaa gactgggctg acacgctgcc
     9481 gcggttgctg gctggagatt acaaggtcga gaatcgtatg atgctgcaag cccagcagtg
     9541 gcgcggcgat gaacgattac gcaccagcca ggtgattaac gaggtggtgg tcgcccgcgg
     9601 acaggtggtg cgtcccatcc gcattcacgc cgaggtggat ggctacccgc tggcgagtta
     9661 cgtggcggat gggctgatcg cctcgactcc caccggctcc acggcttacg cgctggcggt
     9721 gggcgggccg atcatgcccc cggaattgcg caatatcctc atcatgccgg tggcgccgca
     9781 tctctccatg gaccgggcgg tgatcttgcc ggaaggcgcc agcgtggtga tcacagtgga
     9841 cagcgaccac gaggccgtga tgagcgtgga cgggcacgaa ccggcgctta tcagcaatca
     9901 agaccgcgtg acggtgactg ccagcgacct ttcggtgaaa ttcgtcgttt ttcaggaacc
     9961 gggttacttc taccgtaacc tgagcaacta tatggaacaa aatccttcta ttggtgattt
    10021 ttaatggcag aagaagcgac ttcagaatca atgcactgtt atgtgcatcc ccagcgcgaa
    10081 acgttgctgc gctgcaaccg ttgcgaaaaa ccaatttgcc tgtcgtgcgc ggtgctcaca
    10141 cccacaggtt accgttgcaa ggaatgcgtg aaagggcagc aaaggatttt tgaaaccgcg
    10201 cgctggtggg attacccgct caccttt
//
LOCUS       DAOSPM010000065        10151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_6614_length_10151_cov_11.699881, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000065 DAOSPM010000000
VERSION     DAOSPM010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6614_length_10151_cov_11.699881"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="PLW25_06615"
     CDS             <1..1273
                     /locus_tag="PLW25_06615"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HOS54195.1"
                     /translation="LKAVDPQSVVRNNLWQIGGELRVGPNAFRLTPKSRFVTAALGKA
                     APAMLTGAIHQLGDYYGEGLCICKHLDSTDNPFPGTRYILGNHPVPGAGSLRAGEALK
                     SLVIGLGEEDIVLLLLSGGGSALATLPASGITLEEMQQLTVTLLHAGADIHEINTVRK
                     HLDLLKGGGLARLAYPARVVTLVLSDVVGNNLEVIASGPSYPDPSTFADALEIVERAA
                     KHGVIPARIRHHLERGCRGELPETLKPIEPDAQRSFNTIIASNVNASAAAVAQAKALG
                     FTAETVTNELTGDARAAGAQIASMVKTRGDLNKPFLLVWGGETTVVVKGRGKGGRNQE
                     LALGAVKELSGVPNILLISLATDGEDGPTDAAGALVTGDTLNRALVKGLNPESYLREN
                     DAYAFFEGIGDILRTGHTGTNVNDLIFVFGF"
     gene            complement(1270..2289)
                     /locus_tag="PLW25_06620"
     CDS             complement(1270..2289)
                     /locus_tag="PLW25_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOS54196.1"
                     /translation="MRKEVVLVTGANGEIGHGLISYLGEKQASRIIALDVQPLEDNLK
                     PYCERAIQGDILDTMLLGRLVADYDIRIIFHLASILSTKAEYNPETAHRVNVEGTLNL
                     LKLAVEQSSWQGKPVKFLYPSSIAAYGLPTLEKKNQSGKVKEYDWNSPITMYGCNKLY
                     CEHLGRYYNFYYRQLAVDEVKHTIDFRCLRFPGLISSVTVPTGGTSDYGPEMLHAAAQ
                     GVPYNCFVRPDTRLPFMVMPDAIKSLLQLAEAPQENLKQLVYNVTSFSPTAEDFFHHV
                     KKAFPDAEINFKPHIARQRIVDSWPGDVEDAAAKRDWGWKPDYDEKRAFEEYLIPCIR
                     QRYAN"
     gene            complement(2371..3291)
                     /gene="secF"
                     /locus_tag="PLW25_06625"
     CDS             complement(2371..3291)
                     /gene="secF"
                     /locus_tag="PLW25_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOS54197.1"
                     /translation="MNILGKRYLFFALSILIILPGLIMLAVNGMPLSIDFKGGTLLEV
                     SFENGVFPSTEAIKKIYDHNGINDLQVQSTTEGTLLLRSSELSDSVRQSVLDVLAEEA
                     GSPVIILKSDTVGPSIGQGVTQRAIQAVALAALAVVIYITFAFRGVPHAFRYGVCAII
                     AMVHDVLIVLALTGYGAKFFGWQVDSLYLTALLTVIGFSVQDKIVVFDRIRENNRILR
                     KLDFEKLANHSIVQTLQRSINTQLMTVEFLLLALALFGGVSLREFAVILLVGLLSGTY
                     SSIFIAAPLLVVWENHEWKTWFTKKHATTS"
     gene            complement(3305..4654)
                     /gene="secD"
                     /locus_tag="PLW25_06630"
     CDS             complement(3305..4654)
                     /gene="secD"
                     /locus_tag="PLW25_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOS54198.1"
                     /translation="MDRRYANLILILVLVAAIIWVDLPRGEGQQGLKIGNFKRTLNPV
                     LGLDLRGGMQVLLSPQKGISATRQDLEDTSKILESRANGLGVSEVIFQVAGDKYILGQ
                     FPGLTNTEDVIAAVKQTGLLEFIDSGSTQLNPGTVVETDYAAAQSGDETPAAEASGQV
                     WHTVMTGVDIKNVNVAADQGIGGYVVNFELTDSGTQVFKDHTAANVGKYLTIVLDKQV
                     ISSPRISTAIPDGKGQISGNFTSESANNLAVTLRYGRLSVPVEVVESRVVGPTLGSDS
                     LTKSLRAGVIGFIIVALFMLIYYRVPGAIAVVAIAIYAALTFALFKLIPVTLTLPSIA
                     GFLLSTGSALDANILIFERLKEELRNGRTLKQAIDLGWKRAWPSIRDSNIATIITSVI
                     LFWFGSTFGATLVKGFALTLALGVMVSLFTAVFVSRTFLGIAVDFIKTDKIKNSFGL"
     gene            complement(4743..5099)
                     /locus_tag="PLW25_06635"
     CDS             complement(4743..5099)
                     /locus_tag="PLW25_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54199.1"
                     /translation="MIGVVGVCASGKSTLIKGLIQQGYRCRHIAQEHSYVPDMWYRLS
                     RPDILIFLQVSYEITLKRKNLNWNKSEYETQLQRLQHARQNAHIIIDTDLLTPEELVA
                     RTISEIQSLTGNSHSS"
     gene            complement(5167..5886)
                     /locus_tag="PLW25_06640"
     CDS             complement(5167..5886)
                     /locus_tag="PLW25_06640"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOS54200.1"
                     /translation="MQIDIAVAKINKYAVSESGDTLEIVERPNGGVSVVMADGQTSGR
                     GAKTISTMVVRKVISLLADGVRDGAAARAASDSLYTERDGKVSACLNILSVDLQTATI
                     VITRNNPTPIFLARHEQVECITSDSVPIGTNRNIRPSITEFPLESGITIVMYTDGIKF
                     AGERSRQPLDICAFLESMLEDQEPTAQEIADGILNQAIRLDNNRPNDDMTVVALRSIP
                     TEVDQIRRMTVRLPVQDIQRF"
     gene            6233..6430
                     /gene="rpmF"
                     /locus_tag="PLW25_06645"
     CDS             6233..6430
                     /gene="rpmF"
                     /locus_tag="PLW25_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOS54201.1"
                     /translation="MVPLPKRKLSKGRRDRRRAHDGLETANLVQCSNCGEMRLPHTVC
                     PNCGQYQGREVIHIEKEAKKK"
     gene            complement(6472..7833)
                     /gene="hflX"
                     /locus_tag="PLW25_06650"
     CDS             complement(6472..7833)
                     /gene="hflX"
                     /locus_tag="PLW25_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOS54202.1"
                     /translation="MAHNTIPTSPPVERAFLVGVELRSEKPLISMEDSLGELALLART
                     VNLEIVGETSQKIDVPNPSTLVGTGKVEEIALLAEECLADVVIFDTELSPRHQRELEE
                     RFGVKLRVLDRTALILDIFAQHARTSEGILQVSLAQYEYRLPRLTRAWTHLARQAGGG
                     GGRAGSIGGVGLRGPGETQLEVDRREIHKRISMLKKELEKVRAHRQRYRAQRKRSRIP
                     LISLVGYTNAGKSTLLNHLTNAGVLVADQLFATLDPTTRRIELPGGHQALITDTVGFI
                     QKLPTQLIAAFQATLEEISEADLLIHVVDVTHPNAIEQARSVEQTLREIHAEQIPVLT
                     ALNKIDRLPEPELTNEFLSGQSRSVGISALKGLGIEELLRAIEEDLFENFSQVSAQIP
                     YTEGQLISLFHEQGQVVRIEHGRKGVFIQGYIPGRLLARFFPYQSASPQDDDETESWS
                     EPN"
     gene            complement(7836..9020)
                     /locus_tag="PLW25_06655"
     CDS             complement(7836..9020)
                     /locus_tag="PLW25_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS54203.1"
                     /translation="MKNSHVFMPAAQRISSFEPYFFASLGKKINQLKQQGMDIIRIDM
                     GSPDLPPADFIIEELVTQSRKSDTHGYSPTGGTQAFKQAVADYYMDRFEVALDTSSEI
                     IGLIGSKEGLFNLSQVILNPGDIALVPDPGYPVYSASGIIAGGEVVYFPLLAENGFLP
                     DLKAIPESVLQKAKILWLNYPNNPTGAVASMAFFEEVIAFARKHHILVAHDAPYVDIC
                     FDGYTAPSLLQVPGAREVAVEFNSLSKTYNMAGWRLGMAVGNAEVIDYLFTYKSQMDT
                     STFSPIFSAGVKALTGSQDWLVERNQVYKDRRDLVVNGLKQAGFTFEAPKAAIYVWAQ
                     LPAGETDSIHFCNRLLEETGISTTPGIVYGKHGEGYLRVSLGTATNRIREAMNRLVDW
                     KR"
     gene            complement(9092..9976)
                     /locus_tag="PLW25_06660"
     CDS             complement(9092..9976)
                     /locus_tag="PLW25_06660"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOS54204.1"
                     /translation="MRTGLIRLILVCLLLVVVGALSTSLFYKPLGREGFNAGRAYQDV
                     LFQTALGPRVPGSQAHEKVIQYFIQELKRAGWQVTLQRGERLGHSATNILASRGTLQP
                     RVIVGAHYDSRMTADREIDQSLRGTPVPGANDGASGAAVLLELARTIPTSNSEGVWLV
                     FFDLEDQGNIESWDWILGSRIFVDHLTTAPEAVIIIDMIGDRNLLIPREMNSDPELTA
                     ELWKLAAELGYENTFLDLPGYRILDDHIPFLEQGIRAVDIIDFDYPHWHTLQDTPDKV
                     SPASLEIVGNVLLAWLKR"
BASE COUNT         2504 a         2525 c         2603 g         2519 t
ORIGIN      
        1 actgaaggca gttgatccgc agtcggtagt gagaaataat ttgtggcaaa tcgggggtga
       61 attacgcgtg ggtccaaacg cttttcggct gaccccaaaa tcaaggttcg tgaccgcagc
      121 actggggaaa gcggcaccgg cgatgctgac cggcgccatc caccagcttg gcgactatta
      181 tggagaggga ttatgcatct gtaaacacct tgattctaca gacaacccgt tccctggtac
      241 ccgttatatc cttggaaatc atccagtacc gggcgcgggc agcctgcggg ctggagaagc
      301 attaaaaagc ctggtcattg gtctgggtga agaggatatc gtgctgctgc tgctttccgg
      361 cggtgggtct gccctggcta cattgccagc cagcggcata acccttgagg aaatgcagca
      421 gttaacagtg accctcttac atgcgggcgc ggatattcat gaaatcaata ccgtacgcaa
      481 gcatctcgac cttctcaaag gaggcggact ggcgcggctg gcgtatccag ccagggttgt
      541 gacactggtg ctgtcagatg ttgtgggcaa caacctggag gttatcgcct ctggaccgtc
      601 ctaccctgat ccttctactt ttgcggacgc tctggagatc gttgaacgtg cagcgaaaca
      661 cggcgtgatt cccgctcgta ttcgccacca tttagaaaga ggctgcaggg gagaattacc
      721 tgaaacgttg aaacccatcg aacctgatgc ccagcgaagt tttaatacga tcattgccag
      781 caatgtgaat gccagcgctg ctgccgtagc acaggcaaag gcgctcggtt ttacagctga
      841 gactgtcaca aacgagttga cgggcgatgc gcgagcagca ggggcgcaga tcgcttcgat
      901 ggtgaagacc aggggcgact tgaacaaacc ctttctgctg gtatggggcg gagaaacgac
      961 cgtggtggtg aaaggacgtg gaaagggcgg caggaatcaa gagctggcgt tgggagcggt
     1021 gaaagaatta tctggagttc cgaacatcct gctcatcagc ctggcaacag atggagaaga
     1081 tggccctaca gacgctgccg gggcgttggt gacaggagat accctgaacc gcgcattggt
     1141 aaaaggcctt aaccctgaaa gctacctgcg cgaaaacgat gcttacgcat tctttgaggg
     1201 aatcggagac atccttcgca ccgggcatac cggcacgaat gtcaacgatt tgattttcgt
     1261 ttttggtttt taattcgcgt acctttgccg gatgcaggga ataaggtatt cttcaaaggc
     1321 acgtttttcg tcgtaatcag gtttccatcc ccaatctcgc ttggccgcgg cgtcttccac
     1381 gtcacctggc caggaatcca cgattcgctg ccgcgcaata tgcggtttaa aatttatctc
     1441 agcgtcaggg aatgccttct tcacgtgatg gaagaaatcc tctgcggtag ggctgaagct
     1501 ggtgacgtta taaaccaact gttttaaatt ctcttgcggc gcctcggcaa gctgcagcaa
     1561 ggatttgatg gcatcaggca ttaccataaa aggtaagcgc gtatcaggac ggacgaagca
     1621 gttatagggt acgccctggg ctgcagcgtg taacatttca ggtccgtagt cactggttcc
     1681 ccctgttgga acagtgaccg atgaaattaa ccccggaaaa cgcaggcaac gaaagtcaat
     1741 agtatgtttt acttcatcca ctgccagttg ccgatagtaa aagttgtaat acctgccaag
     1801 gtgttcacag taaagcttgt tgcagccgta catcgtgatg ggtgagttcc aatcgtattc
     1861 tttaaccttg ccagactgat ttttcttttc gagtgtgggt aacccatatg cggcaattga
     1921 actgggataa aggaacttga ccggttttcc ctgccaggaa gattgttcaa ccgccaattt
     1981 gagcaggttc agcgttcctt caacattaac ccggtgagcg gtttcagggt tgtattctgc
     2041 ttttgtggaa agaatggacg ccagatgaaa gattatgcgg atatcataat cagccaccag
     2101 tcggcccaga agcatggtat caagaatgtc accctggatc gcccgctcgc aataaggttt
     2161 cagattgtct tccaagggct gaacatcaag ggcgatgatc cggcttgctt gtttttcacc
     2221 cagatagctg atcaatccat gacctatctc tccattcgcg ccagtgacca agaccacttc
     2281 ttttcgcatg cgcatccctc ctgttcagta ggttgactgc ttacttgcct agtgtaaaaa
     2341 agttctggat tgctccagaa ctcttttgat ttacgaagtc gttgcgtgtt tctttgtgaa
     2401 ccaggtcttc cactcatggt tttcccaaac caccaggagc ggagccgcga tgaagatgga
     2461 ggagtaagtt ccgcttaata aaccaaccag gaggatcaca gcaaactccc gcaggctgac
     2521 gccgccaaaa agcgccaaag cgagcaacaa gaattcaacc gtcatgagct gggtgttaat
     2581 ggaacgctgc agcgtctgca caatggagtg gttcgccagc ttctcgaagt ctaacttgcg
     2641 aaggatacgg ttgttctcgc ggatgcggtc gaacaccacg atcttatcct ggactgaaaa
     2701 accgatgact gtaagtagag cggtaaggta taaagaatcg acttgccaac cgaagaactt
     2761 ggctccgtaa ccggtcaagg cgagaacgat gagcacatcg tggaccatgg cgataatggc
     2821 gcacacgcca taacgaaaag cgtgcggtac cccgcgaaag gcgaaggtga tgtaaattac
     2881 caccgccaaa gctgcaagcg cgaccgcctg gattgcccgc tgtgtgaccc cttgaccgat
     2941 agaagggccg actgtgtctg attttagaat aatcaccggg ctgccggctt cttctgccaa
     3001 gacatccaga acggattggc gcacactatc acttaattct gatgagcgta aaagcaaagt
     3061 gccttcggtg gtcgattgaa cctgaaggtc attaatgcca ttgtggtcat agatcttttt
     3121 tatcgcttcg gtggaaggga aaacgccgtt ctcaaaactc acttcaagca atgtgccgcc
     3181 tttgaaatca atcgataaag gcattccatt caccgcgagc atgatcaacc ctggcagaat
     3241 aatgaggatg gagagtgcaa aaaagagata tcttttacca agtatgttca ttattatttt
     3301 ccttctacaa accgaatgaa ttcttgatct tatcggtttt tatgaaatcg accgcgattc
     3361 cgaggaaggt tcggctgaca aacacggcgg tgaacaatga aaccatcact cccagcgcca
     3421 gagtgagggc aaagcctttc accagtgtag cgccaaatgt tgatccgaac cagaaaagga
     3481 tgacgctggt gatgatcgtg gcgatgttcg aatctcgtat ggaaggccag gcgcgcttcc
     3541 atcccagatc aatggcctgt ttcagggtgc gcccgttacg gagttcttcc ttaagtcttt
     3601 cgaaaatcag aatgttggcg tccagcgcac tgccggtgga taagagaaag cctgcgatgc
     3661 tgggaagcgt gagggtgaca ggaatcagtt taaataaggc gaacgtgagc gctgcgtaaa
     3721 tagcgatagc gacaacagcg atagcccctg gaacacgata ataaattaac atgaacaggg
     3781 ctacaatgat aaagccgatg acgcccgctc taaggctttt cgttagtgag tctgacccaa
     3841 gggttgggcc gacgacacgg ctttctacta cttcaacagg cactgaaagg cgcccatagc
     3901 gcagggttac tgccaggtta ttggctgatt cgcttgtgaa attgcccgaa atttgcccct
     3961 ttccatctgg aatggcggtg ctgattcgcg ggctggagat tacctgtttg tcgagcacga
     4021 tggtcaggta cttaccgaca ttggcagcgg tatggtcttt aaatacctgt gtgccgctat
     4081 cagtgagttc gaaattgaca acatacccgc caatcccctg gtctgcagcg acgtttacgt
     4141 ttttaatatc cacgccggtc attaccgtat gccagacctg tccggacgct tcggctgctg
     4201 gagtttcatc gccagactgg gcagcagcgt aatccgtttc taccactgtg ccagggttta
     4261 actgcgtgct acctgaatcg ataaattcga gtaaaccagt ttgcttaacc gcggcaatca
     4321 catcttctgt attggtcaat ccggggaatt gcccaagaat atacttatct cctgcgacct
     4381 ggaagatcac ctcgctcaca ccaagcccgt tggcgcggct ttccaggatc ttgcttgtgt
     4441 cttcaaggtc ctggcgtgtg gcagagatcc ctttttgggg gctgaggagc acctgcattc
     4501 caccgcgcag gtccagcccg agcacagggt taagtgtgcg cttgaaatta ccaatcttta
     4561 agccctgttg gccttcacca cggggcaggt ccacccaaat aatggctgcg acgaggacga
     4621 ggatcaggat cagatttgca taacgacgat ccatgtgatg ttctacctcc aatgcgatta
     4681 aaatcctgcc gaaaaaaggc gggaactcag ggattatacc tcaaaccatt ataaaagttg
     4741 acttaactgg aatgagaatt ccctgtcaga gactggatct cacttatcgt tctcgcaaca
     4801 agctcttccg gcgtcaggag gtcagtatcg ataatgatgt gagcattctg cctggcgtgc
     4861 tgaagccttt gcagctgggt ttcgtattcg ctcttgttcc agttaagatt cttgcgttta
     4921 agtgtgattt cgtaggatac ctgtaaaaag atgagaatat ccggcctgga aaggcggtac
     4981 cacatgtctg gtacataaga atgctcttga gcgatgtgcc tgcatcgata cccttgttga
     5041 attaaccctt tgatcaaggt ggatttaccg gaggcgcaaa cgcctacaac ccctatcaaa
     5101 aggtcagttc ttatagagct catggttgga aaggaggagg agttctcctg aactttgaaa
     5161 actcggttaa aacctctgga tgtcttggac gggcagtctg acggtcatac gccttatctg
     5221 gtcaacctca gtagggatcg agcgcagggc gaccacggtc atatcatcgt tcggccgatt
     5281 attatccagc ctgatggcct ggttcaggat gccatcggca atttcctggg cggttggctc
     5341 ctggtcttcc aacatggatt caaggaaggc gcagatatcc aatggctgcc gagagcgttc
     5401 cccggcgaat ttgattccat ctgtatacat cacgatggta atgccgcttt ccagggggaa
     5461 ctcggttatg gaagggcgga tgttgcggtt ggtaccaatg ggaacactat cagaggtaat
     5521 gcactccact tgctcgtgtc ttgccaggaa aatgggggtg ggattgttgc gggtaatgac
     5581 gatggtggcg gtttggaggt caacagaaag aatgttcaaa caggcggata ctttcccatc
     5641 ccgctctgtg tacaacgaat cagaagcggc gcgggcagcg gctccatctc gcacaccgtc
     5701 tgccagcagg ctgatcacct tacgcacgac catggtggag atggttttcg cgccgcgacc
     5761 ggaagtctgc ccatctgcca tcaccactga tacgccccca tttggtcgtt ccacaatttc
     5821 cagcgtatca ccgctttcag atacggcata tttgttgatt tttgccaccg cgatatcgat
     5881 ttgcatgcat agattttaca tcaaaatact gtttctttgt ttttccgttg attacctgat
     5941 gcgccttgct gcacgcttat ccccagccgt ttggcctcct ctatttgcgt aatctgtttc
     6001 ctcgccatct gactcatgat ttctgtcctg cgtatctctt tggcactcat gttgtagatt
     6061 ctgtccactt tcagcctcca catatgggga cattttaact ttggtaaaag gggacatttt
     6121 aactttggtt taacaactaa cacggtaagg ttgacggcaa cgggcaagtt atgtataatc
     6181 acccggcctg tcaaaacatg acgggtgagg aatttttgac aggagaagag aaatggttcc
     6241 gcttccaaaa agaaagcttt ctaagggtag aagagaccgc cgtcgcgcgc atgatgggct
     6301 cgagaccgcg aatcttgttc aatgcagcaa ttgtggagag atgagactcc cgcatacggt
     6361 ctgccccaac tgtgggcagt atcaaggaag agaagtcatc catattgaaa aagaagccaa
     6421 gaaaaagtga ttaaaaacat caatgacggg cgacacaagc ccgtttttga tttaattcgg
     6481 ttctgaccat gattcagttt catcatcgtc ttgtggtgac gctgactggt atgggaagaa
     6541 tcgcgccagc aaccgcccgg ggatatatcc ttgaataaag acgcctttac gtccatgttc
     6601 aatacgcacc acctgacctt gctcgtgaaa cagagaaatt aattgtcctt ccgtataggg
     6661 aatctgagcg gaaacctggc tgaaattctc aaacaaatct tcctcaatcg ctctcagtaa
     6721 ttcttcaatt cccaatccct ttaatgcgga aattcccacc gatcgtgatt gaccggatag
     6781 gaattcattg gtaagttcag gctctggcag ccggtctatc ttattaaggg cggtaagcac
     6841 tgggatttgc tcggcgtgga tctcgcgcag tgtctgttcc actgacctgg cctgttcaat
     6901 ggcattggga tgcgtcacat ccaccacgtg gattaacagg tccgcttcgc ttatttcttc
     6961 aagggttgcc tggaaggctg cgatcaactg agtcggcagt ttttggatga agccgaccgt
     7021 gtcagtaatc agcgcctggt gcccgccggg gagttcaatt cttcgcgttg tcgggtcaag
     7081 ggtggcaaaa agttggtctg cgaccaacac gccagcgttg gtgaggtggt ttagaagggt
     7141 ggatttacca gcattggtgt atccaaccag gctgattaga gggatgcgtg aacgctttcg
     7201 ctgcgcgcgg taacgctggc ggtgagcgcg gactttttca agttccttct tgagcattga
     7261 aatgcgtttg tgaatttccc gccggtccac ctctaactgg gtttctccgg gcccacgtaa
     7321 gccgacgccg ccaatcgaac cagcgcgccc accgccgccg cccgcctgcc ttgccaggtg
     7381 ggtccaggcg cgggtcagcc tgggcaggcg gtactcgtat tgcgctaacg atacctgcaa
     7441 gataccttca cttgtgcgcg cgtgctgggc aaaaatatcc agaattaatg ccgtgcggtc
     7501 aagtacgcgt aatttaacac caaacctctc ttccagctcg cgctgatgcc tgggagaaag
     7561 ttcagtatcg aagatgacca catctgccag gcattcttcc gccaataagg caatttcctc
     7621 gacctttcca gttccaacca gcgtgctggg attgggaaca tcgatttttt gagaggtctc
     7681 gcctactatc tccaggttca ccgttctggc cagcaaggcg agttccccca gtgaatcttc
     7741 cattgagatc agcggttttt cactacgaag ttccactccc acgagaaaag cccgctcaac
     7801 aggagggctg gtgggtatgg tattgtgagc catgcttatc ttttccaatc aaccaggcgg
     7861 ttcatcgctt cgcggatgcg attggttgcg gtacccaaag aaacccgcaa atagccttca
     7921 ccatgttttc cgtagacaat accaggcgtg gtactgatgc cggtttcttc taacaaccgg
     7981 ttacaaaaat ggatggaatc tgtctcgccg gcagggagtt gcgcccacac gtaaatggcg
     8041 gccttaggag cttcaaaggt gaagcccgcc tgcttcaatc cgttcacaac cagatcccgc
     8101 cggtctttgt agacctgatt gcgctcaacc aaccaatcct ggcttccggt caacgctttg
     8161 acccctgcgg aaaaaatggg agagaatgtg gatgtatcca tctggctttt ataagtgaag
     8221 agataatcga tcacttccgc gttgcccact gccataccca aacgccaccc tgccatgtta
     8281 tatgtttttg aaagtgagtt aaattcaacg gctacctctc tggcgcctgg tacctgcaag
     8341 aggctggggg ctgtgtaacc atcgaagcaa atatccacgt atggggcgtc atgagccacg
     8401 aggatgtggt gcttgcgggc gaaagcgatg acttcctcaa aaaacgccat actggcgaca
     8461 gcgcctgtag ggttattggg gtagttcagc cagaggattt tggctttttg cagcacactt
     8521 tcaggaatcg cctttaggtc gggcaggaag ccattctcag ctaataaggg gaaatagacc
     8581 acctcgccgc cagcgatgat accgctggcg ctgtaaacgg ggtagccagg gtcaggtacc
     8641 aatgcgatat ctccagggtt gaggataacc tggctgaggt taaatagccc ttcttttgag
     8701 ccgatcaagc cgataatttc actggaagtg tccagcgcaa cctcaaaacg atccatgtaa
     8761 tagtcagcca ccgcctgttt gaaagcctgt gtccctccag taggagaata accatgcgta
     8821 tcagattttc gactttgtgt caccaactct tcgataataa aatcagcggg tgggaggtca
     8881 ggcgacccca tgtcaattcg gataatatcc ataccctgct gtttcaattg gtttatcttt
     8941 ttccccaaag aagcaaaaaa gtagggctca aacgatgaaa tccgttgggc agcaggcatg
     9001 aagacatggc tatttttcat agttgttgtt ttccttcggt tgttttaata gtatcagtag
     9061 tgtaagtcat ttacggtcaa ttgacaatca cttaacgctt cagccaggcc agcagtacat
     9121 tgcccactat ttcaaggctg gctggagaga ccttatcggg agtgtcctgg agggtgtgcc
     9181 agtggggata atcaaaatcg atgatgtcga ccgctcgaat accctgttca agaaaaggaa
     9241 tgtgatcatc aagaatcctg tacccgggca agtcaagaaa agtattttca taacctaatt
     9301 ccgcagccag cttccagagc tctgcggtca gttcggggtc tgagttcatt tctcgaggaa
     9361 tgagcaaatt tcgatcgcca atcatatcga tgatgatcac tgcttctgga gccgttgtta
     9421 gatggtcaac aaaaatacgc gaacccagaa tccagtccca actctcaata tttccctggt
     9481 cttctaaatc aaagaaaacc aaccagactc cctcactgtt agaagttggg attgttctgg
     9541 ccagttcaag cagcaccgct gcgcctgatg cgccatcgtt tgcgccagga acgggcgtac
     9601 ctcgtaggct ttggtcaatc tcccggtcgg ctgtcatccg gctgtcgtaa tgcgctccaa
     9661 cgatcacccg cggctgcagg gtgcccctgc ttgcaaggat attggtggct gagtgcccca
     9721 acctttcacc acgctgaagc gtcacctgcc acccggctcg ttttaactcc tgaatgaaat
     9781 attggataac tttttcgtgc gcctgagaac caggcacgcg aggaccaagc gctgtttgaa
     9841 agaggacatc ttgatacgcg cgtccggcgt tgaaaccctc tctgcctaaa ggtttataga
     9901 aaagagaagt gctcagagct ccaacaacca cgaggagcaa acaaaccagt atcaaacgga
     9961 ttaggccagt tctcattatt gcgcctttat taatcatcgc tttagcgatg atttcacgac
    10021 cattttaacc gaaaaacgct ggcatagaac ttgcacgatt gctttaaaaa ggctatactt
    10081 cattaggatt tgaatactat gatacagcaa acccgccagg ttcaaaaacc gctcacaact
    10141 gcgcaccttg c
//
LOCUS       DAOSPM010000066        10039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_6706_length_10039_cov_18.453025, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000066 DAOSPM010000000
VERSION     DAOSPM010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6706_length_10039_cov_18.453025"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..501
                     /locus_tag="PLW25_06665"
     CDS             31..501
                     /locus_tag="PLW25_06665"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOS54205.1"
                     /translation="MQLRSIVFMLGFILLGCVFMFRAVSGRLKSKKAQAWPVVKGRVV
                     SSEVEEDRFRSATGKATIAFVPDISYEYQVNGKSYTSRTVIFGATTYDYLTASRICEK
                     FSVGSEPKVYYNPANPQDAVLLPQATEGLRSLIPGTFFIVTGIAIGVMGFLFPN"
     gene            658..1122
                     /locus_tag="PLW25_06670"
     CDS             658..1122
                     /locus_tag="PLW25_06670"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOS54206.1"
                     /translation="MLGLIIGFSLLLTGVVFLIIGLVAAKRAKAAQSWPTIPGRVLSS
                     QVVEHESTDSEHGTTTVTYEPRVEYEYIVMGQAYTAKRIAFGANRFHYKKAEEIAARY
                     PVGAQVNVRYNPDKVKDATLEANAAGGKLFLILGGVFGAVGLVTMVISLFSL"
     gene            1213..2223
                     /gene="galE"
                     /locus_tag="PLW25_06675"
     CDS             1213..2223
                     /gene="galE"
                     /locus_tag="PLW25_06675"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOS54207.1"
                     /translation="MKYLITGGAGYIGSTICSALEDAGHVPVVLDSLITGREEFTRGR
                     IFYQADIADERAVTQLFREHPDIQATVHCAALIVVPDSVSSPYEYYQDNVSKSIEFFH
                     LLHRLGYDRVVFSSSAALYDVVPGFMVTEEAPLNANSPYSRTKLMMEMVLRDYCAAYG
                     MKGIALRYFNPIGADPKMRSGIHVQHPTHVVGKLVDTAQGRQSEFQITGTHYPTRDGT
                     GIRDYIHVWDLAQAHVNAVTDFDKVFERAGELPVDYLVINLGTGRGVTVRELVTAFEK
                     VYGQTINKKETGPRPGDVAGSYTNADKAYRLLHWKAQLSIEEGIADALKWGKLRENIL
                     KY"
     gene            complement(2268..3332)
                     /locus_tag="PLW25_06680"
     CDS             complement(2268..3332)
                     /locus_tag="PLW25_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HOS54208.1"
                     /translation="MTHLFDPLKIKDIILRNRVGVSPMCQYSYTDGFSNDWQVIHLGA
                     RAVGGAGLVIAEATAVEARGRITPFDVGIWRDEHIDPLRRVVKVVKENGAVPGIQIAH
                     AGRKACVGQPWTGGRPVQEGDPLSWPVVGASPIAFSEEHQVPNELTVEEISAIRKRFV
                     EGAQRSLAAGFEWLEIHGAHGYLLQSFCSPLSNQRGDQYGGVLENRVRFLLEVVRDVR
                     RVWPERLPLTVRISGTEWMEGGWNVQDSLVLARLLKEEGVDLVDCSSAGNLEKAPVPT
                     DPGYQVHIAEAVRRGAGILTAAVGLITTAQQAEEIIHSGSADLVLLGREMLRSPYWAI
                     LAAKELGQAPPVPPQYLRAF"
     gene            complement(3361..4383)
                     /gene="holA"
                     /locus_tag="PLW25_06685"
     CDS             complement(3361..4383)
                     /gene="holA"
                     /locus_tag="PLW25_06685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOS54209.1"
                     /translation="MTDAHVHLLRGDDAFAISLQVKSIIASLGADFDSSMNYSRLDGK
                     SVSLEDMRLAVSTLPFFGGRRLVVIDDAISKVEKSRVPAFTAMLASAPPTTTLVIIVE
                     DTQKWRKVENEWTRVWETLTPAHWLVQWCSSHPQACIVDLGLPDAKNMDAWILNETKR
                     QEGKMEPSAAYELSRLTGIDTSIASQEIAKLLMHVDFQRPVTREDVLELVSDEGSVDV
                     FAMLDSLMEGKTRNAQAMMRRLLDDTPPEVVLGALAHRLRQLILLAEAMDSGEDARSL
                     ARKSGIFINKIESSRNAVRCIGISGLEELYHHLLEIDLQAKTSGTDLATNLELLVLKT
                     GHYFKK"
     gene            complement(4387..4926)
                     /locus_tag="PLW25_06690"
     CDS             complement(4387..4926)
                     /locus_tag="PLW25_06690"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HOS54210.1"
                     /translation="MAELNKVLDWAPVSMIRRNHGLEHATLNLLAAEFPSCSFAGHSD
                     SKGFWIIGEVSTDKLLETVQQALKRMKAGESDLALHTNCGTNYVTTGLIAGSVAWLGT
                     LGNANSFSKKLNRWPLLVMLITGSLILAQPLGKKVQARISTSSDPGNLVVKQITRYER
                     KGPTLHRVLTTDILEITES"
     gene            complement(4954..6363)
                     /gene="radA"
                     /locus_tag="PLW25_06695"
     CDS             complement(4954..6363)
                     /gene="radA"
                     /locus_tag="PLW25_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOS54211.1"
                     /translation="MPKTISQYVCQQCGKVAPRPLGKCPQCGGWDTMVEEVLQAPAAP
                     AKTHLRGLNDRSHPRRMAEIQGNAEERISLPIGEFARVLGGGVVPGSIVLVGGDPGIG
                     KSTLLLQMALEMAGDHSARKVLYVSGEESERQIKMRALRLTRQPGEKSAGFSDDLLLL
                     TETNLEAILEHIGALKPDLLIVDSIQTVYLPEMSSTAGSVSQVRECANRLRELAKSSG
                     MAVFLVGHVTKEGVIAGPRVLEHIVDTVLYLEGDRFQSYRLLRSVKNRFGATAEVGVF
                     EMREQGMVEVLNPSEAFLAERLVNAPGSAIAVTMEGTRPLLVEMQGLTSPSNLGNPRR
                     TPNGVDFNRLLLITAVLTRRLGLRLAEQDVFVNVIGGLQVNEPAADLAIAASIASSMK
                     DIPVKADMVLIGEVGLSGELRWVGQMNARLREAAKLGFKTALIPRRVRPGEPLPKEIE
                     VKEARSLREALGFALLTEK"
     gene            complement(6647..7768)
                     /locus_tag="PLW25_06700"
     CDS             complement(6647..7768)
                     /locus_tag="PLW25_06700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS54212.1"
                     /translation="MAVEIREVTTIKGLKKFIRYPLTLYKGSPYYVPNLFADELNTLR
                     ADKNPAFEDARARYWMAYRENQAVGRVAAMIVPRHEEKWGEKYMRFGWIDFIDDAEVV
                     KALMDTVENWARQEGKLGVHGPLGFTDLDREGMLVEGFDEMTTLATIYNYPYYPQRLE
                     ELGYTKDVDWIEHEFTFSTQLEEKIANAAALIAQRTKTHLFQGKKNDLLGLAPQIFDV
                     INEAYRHLYGTVPLTEAQVKSYVNTYFGFANPDFIPLVMDENERLVAFGITFPSFSRA
                     LQKSKGNLFPFGFIHFLRALHKNDRADLYLVGVRDEYRGRGITALLMHQIFQTFKKHG
                     IRYIETNATLENNQDILAMWKYLDSRQHKRRRAYVKHLA"
     assembly_gap    7831..7930
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7931..9204)
                     /locus_tag="PLW25_06705"
     CDS             complement(<7931..9204)
                     /locus_tag="PLW25_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54213.1"
                     /translation="MKNRIESVLLYLLPSVGAILWISIFFGVLMSGPRMVNADGDLGR
                     HITIGGYILDNHLVPLRDVFSHTMLGEPLTPHEWLAQVLFAQVHRLMGINGAVLLAAL
                     VIATAFWLVYRQTRAQSGSLLAAAMVSILSLLASSIHWLSRPHIFTFLLLALWMGVLY
                     QIKQGNTRRWWLMPLLMIAWVNLHGAFIAGFATWGLCAFGMLWERWFTRSEEREVPPA
                     GFWKSYLLGGGFSLLATLLNPAGLGIWGTSVGYVANRYLVDHTVEYQSPNFHEPITWL
                     LLLFIALLIVVLALRKRHTTSFQLFPAVAWLVMSLYSARNIPLFAIVAAPLLAKACVD
                     FLSGLELPEILRRRVEKLDAGLLKVDLRLKGIFWPVLSVVIVVIGFSAGMTFTRDGTA
                     NAYDPAKFPVGAVDWLEQNPQRGEVFNYFPWGG"
     gene            complement(9278..>10039)
                     /locus_tag="PLW25_06710"
     CDS             complement(9278..>10039)
                     /locus_tag="PLW25_06710"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOS54214.1"
                     /translation="DFAGYTAIVRGVSLWFGIELSANFNLPYYARNFTEFWNRWHITL
                     SNWLRDYIYYPLAWSLRRRFHQQDHFFNIFLPPMVTLLVSGLWHGLSWSLLVWGALHG
                     LYLVLERLPSLGKAVVPVNKRPLWRQRLGTAVTFLFASLAWVPFRMPLPAALQYWQGL
                     LRWSMPDFIGLARTLVGWSPVSGWSQFDLPNPILLLVLAAALLFDQLQHRAGTEEFML
                     NQPRWLQVLLTALLLLVATLAFFSDTTAPFVYQGF"
BASE COUNT         2178 a         2889 c         2827 g         2045 t
ORIGIN      
        1 taaaaccatt ctcaattacg ggaggaaggc atgcagctgc gttcgatcgt ttttatgctg
       61 gggttcatct tgctgggatg cgtgttcatg ttccgggcgg tcagtggacg cctgaaatct
      121 aaaaaggcgc aagcctggcc ggtggtgaag ggcagggtgg tctcttccga agtagaagaa
      181 gaccgctttc gcagcgccac agggaaagcc accatcgcct ttgtgcctga tatctcctat
      241 gagtaccagg tgaacggaaa aagctatacc agccgcacgg tgatttttgg cgcgaccacc
      301 tacgattacc tcaccgcttc gcgcatctgc gaaaagttct ccgtgggcag tgaacccaag
      361 gtgtactaca accccgccaa cccgcaagac gctgtgctgc tgccgcaggc caccgaaggg
      421 ctgcgttcgt tgatccccgg cacctttttc attgtgacag ggatcgcgat tggcgtgatg
      481 ggatttttat ttccgaacta aaaactttaa ccgatggacg ctcgtgggta agtgaaaaag
      541 gatgattggc gcgaggatga aatgaacgag agcgacccac acgttgtgtg gttgtgaagc
      601 cgcctgccct gggaaacccc gcggcagcaa gcgctaactt gaaaaaggag ggttcttatg
      661 cttgggttga tcattggttt ttccctgctg ttgactggag tcgtttttct catcattggc
      721 ctggttgcgg caaaacgcgc aaaggccgcg caatcctggc cgactattcc cggcagggtg
      781 ttgagctcgc aggttgtcga acacgagagc acagacagtg aacacggcac cacgacggtc
      841 acttacgagc cgcgggtcga gtacgagtat atcgtgatgg gccaggcata caccgccaag
      901 cggattgcct ttggcgccaa tcgttttcac tataaaaaag cggaggagat cgccgcgcgt
      961 tacccggtag gcgcacaggt gaacgtgcgc tataacccgg ataaagtgaa agacgccacc
     1021 ctcgaagcca atgccgctgg cggtaaactc tttctcatcc tcggcggggt ctttggcgcg
     1081 gtggggctgg tgacgatggt catctcgttg ttctctctct aattatttct agaactaaac
     1141 ctgcgcgggc ggcaggtgtt ggtatactaa gcctgttttg cattacccgg ttaccgctct
     1201 tggaggaaag gcatgaaata tctcatcact ggaggcgccg gttatattgg cagcaccatc
     1261 tgttccgcgc ttgaagacgc ggggcatgtt ccggtcgtgc tggattcact gatcaccggt
     1321 cgcgaggagt ttacgcgcgg gcgcattttt taccaggcgg atattgctga cgagcgcgcg
     1381 gtcacacagc tcttccgtga gcaccctgat attcaggcca cggtgcactg cgctgccctc
     1441 atcgtcgtgc ccgactccgt cagcagccct tacgagtatt atcaagataa cgtctccaaa
     1501 tcgatcgagt ttttccattt attgcaccgc ctgggttatg acagagtggt gttcagttct
     1561 tccgccgcgc tttatgacgt ggtgcccggc tttatggtga cggaagaagc ccctttgaat
     1621 gccaatagcc cctattcgcg caccaaactg atgatggaaa tggtcctgcg agattactgc
     1681 gcggcttacg gcatgaaagg catcgcgctg cgctacttca accccatcgg cgcggacccg
     1741 aaaatgcgca gcggcatcca tgtgcagcat cccacgcacg tggtaggcaa actggtggat
     1801 accgcccagg ggcggcagtc ggaatttcag atcaccggca cgcactaccc cacccgcgac
     1861 ggcaccggca ttcgtgatta catccatgtg tgggacctgg cgcaggcgca tgtgaacgct
     1921 gtaaccgatt tcgataaggt ctttgaacgc gccggagaac tgcccgttga ttacctggtc
     1981 atcaacctcg gcactgggcg cggcgtgacc gtgcgtgagc tggtgaccgc ctttgaaaag
     2041 gtttatggtc aaacgatcaa caaaaaagag accggtccac gccccgggga cgtggcaggt
     2101 tcttacacca acgccgataa agcctatcgg ctgctgcact ggaaagcgca actttccatc
     2161 gaagagggta ttgcagatgc cctcaagtgg ggaaaactgc gcgaaaacat tctcaagtat
     2221 taaataacaa cggctggagc acctccagcc gttgttttat gggtttatca aaaagcgcgc
     2281 aggtactgcg gcggcacggg cggcgcctgg ccgagttctt tggcagcgag gatggcccag
     2341 taagggctgc gcagcatttc ccgccccagc agtaccaggt cagcgctgcc gctgtggatg
     2401 atctcctcag cctgctgagc cgtggtgatt agcccgacag cggcggtgag aatacccgcc
     2461 ccccggcgca ctgcttcggc gatgtgcacc tggtagcctg ggtcggtggg tacaggcgct
     2521 ttctccaggt tgcccgccga tgaacaatca accaggtcta ccccctcttc tttgagcaaa
     2581 cgagccagca ccagcgagtc ttgcacgttc caaccgccct ccatccactc ggtacccgaa
     2641 atgcgcaccg tcagcggcag gcgttccggc caaacacggc ggacatctcg caccacctcc
     2701 agcaagaagc gcacgcggtt ttcaagcaca ccgccgtact ggtcaccccg ctggttggaa
     2761 agcggcgagc agaaggattg caacaagtag ccatgcgctc cgtgaatctc caaccactcg
     2821 aaacccgctg ccaggctgcg ttgggcgcct tccacaaagc gcttgcggat ggcggagatc
     2881 tcttccacgg tcaattcgtt cggcacctgg tgttcttctg aaaacgcgat tgggcttgcg
     2941 cctacgaccg gccacgacaa cgggtctccc tcctgcacgg ggcggcctcc cgtccacggt
     3001 tgccccacgc aggccttgcg cccggcgtgg gcgatctgga tacccggtac ggcaccgttc
     3061 tctttcacca ccttcaccac ccggcgcagc gggtcgatgt gttcgtcgcg ccagataccg
     3121 acatcaaagg gggtgatgcg cccgcgcgct tccaccgctg tggcttccgc gatgaccagt
     3181 ccagcgccgc caacagcgcg cgcccccagg tgaatcacct gccagtcatt ggaaaagccg
     3241 tcggtgtaag agtactgaca cataggtgaa acccccacgc ggttgcgcag gatgatgtct
     3301 ttaatcttta atgggtcgaa taaatgggtc atgttttcct cgtcaggtct aaaaggagat
     3361 ttatttcttg aaataatgcc ctgttttcaa gaccaaaagc tcaaggttgg tggcgaggtc
     3421 agtccccgaa gttttggcct gcaggtcgat ttcgagcagg tggtggtaca gctcttccaa
     3481 cccagaaatg ccgatgcagc gcacggcgtt acgggaactc tcgatcttgt tgatgaaaat
     3541 gccggacttg cgcgccaggc tcctggcgtc ttcgccgctg tccatcgctt cagccaacag
     3601 gataagctgg cgcaggcggt gagcaagagc gcccagtaca acctccggcg gcgtgtcatc
     3661 cagcaggcgc ctcatcatcg cctgggcgtt gcgggttttg ccttccatta acgaatcgag
     3721 catggcaaac acatccaccg aaccttcatc agagaccaat tccagcacat cttcgcgcgt
     3781 caccgggcgc tggaaatcca catgcatcag cagtttggct atctcctggc tggctatact
     3841 ggtatcgata ccagtgaggc ggctcaactc atacgccgcg gagggttcca tcttcccttc
     3901 ctgccgtttg gtttcgttga ggatccaggc atccatgttt tttgcatccg gcaggccgag
     3961 gtccacaatg cacgcctgcg ggtgcgaaga gcaccactgt accagccaat gcgcgggcgt
     4021 gagggtctcc caaacgcgcg tccactcgtt ttcaaccttg cgccattttt gtgtatcttc
     4081 gacgatgatc accaatgtgg tggtcggtgg cgcggatgcg agcatcgcgg tgaaggcagg
     4141 cacacggctc ttctccactt tgctgatggc atcgtcaatt accaccagcc gcctgccgcc
     4201 aaaaaaaggc agcgtggata ccgccaaccg catatcttcc agcgagacgc ttttcccatc
     4261 caggcgtgaa taattcatgg aggagtcgaa atccgcgccc aggctggcga tgatgctctt
     4321 tacctgcagc gagatggcga aagcgtcatc tccgcgcagc aggtgaacgt gcgcatcggt
     4381 caagggtcag ctctccgtga tttccagtat atcggtggtg agcacacggt gcagcgtggg
     4441 acctttacgt tcgtagcgcg tgatctgttt cactaccagg ttgccggggt cactcgaggt
     4501 tgagatgcgc gcctgcacct tttttccgag aggttgggcc aggattaacg aaccggtgat
     4561 gagcatcacc aataatggcc accggttgag ttttttcgag aaagaatttg cgttgcccaa
     4621 tgtgcccaac caggcaacgg agccggcgat gagcccggtg gtgacataat tggtgccgca
     4681 attggtgtgt agcgccaaat cgctctcgcc tgctttcata cgcttaagcg cctgttgcac
     4741 cgtttccagc aatttatcag tcgaaacttc cccaatgatc caaaagccct tgctgtcgga
     4801 gtgcccggca aacgaacaac tcgggaattc agcggccaag agattaagcg tggcgtgttc
     4861 aaggccgtgg ttgcgcctga tcatggatac cggggcccaa tcgagcacct tgttaagttc
     4921 agccatatat cacctccgag gtcatggaac gtatcatttc tccgtaagca gcgcgaagcc
     4981 caaggcttca cgcaacgaac gggcttcttt cacttctatt tctttgggca gtggttcacc
     5041 agggcgcacg cgccgcggga tgagggcggt tttaaacccc agtttggcgg cttcgcgcag
     5101 gcgggcgttc atctgcccca cccagcgcag ttctcctgaa agccccacct cgccaataag
     5161 caccatatcg gctttcaccg ggatgtcttt catcgaagac gcgatggaag ccgcgatcgc
     5221 caggtcggct gcgggctcgt tcacctgcag cccgccgatc acattgacga aaacatcctg
     5281 ttcagccagg cgcagaccca ggcggcgcgt gagcaccgcg gtgatgagca gcaggcggtt
     5341 gaaatctacc ccgttggggg tgcgccgcgg gtttcccagg ttgctgggac tggtgagccc
     5401 ctgcatttcc accagcagcg ggcgggtgcc ttccatggtg acggcgatgg ccgaccccgg
     5461 ggcgttgacc aggcgttcgg ctaaaaaggc ttctgagggg ttgagcactt cgaccatgcc
     5521 ctgttcgcgc atttcaaaca cgcccacttc ggcggtggcg ccaaaacgat ttttcaccga
     5581 acgcagcagg cggtacgatt gaaaacggtc gccctccagg tagagcaccg tgtccacaat
     5641 gtgctccagc acgcgcggac cggcgataac gccttcttta gtgacatgac ccaccaggaa
     5701 caccgccatg ccgctggatt tggccagctc acgcagacgg ttggcgcatt cgcgcacctg
     5761 gctgaccgac ccggcggttg agctcatctc gggcaggtac accgtctgga tggaatccac
     5821 gatcaacagg tctggtttaa gcgcgccgat atgctccagg atggcttcaa ggttggtttc
     5881 ggtgagcagc aacaggtcat cactgaagcc agcggatttt tcacccggct ggcgggtgag
     5941 gcgcagggcg cgcattttga tctggcgctc tgattcttca ccagatacat acagcacctt
     6001 cctggcgctg tggtcgcccg ccatttccag cgccatttgc agcagcagcg tggatttacc
     6061 gatgcccggg tcgccgccca ccaggacaat ggaacccggc accacgccgc cgcccaacac
     6121 gcgcgcgaac tctccaatgg gcagggagat gcgctcttcg gcgttcccct ggatctctgc
     6181 catgcgtcgc ggatgtgaac gatcgttcag tccacgtaag tgggttttgg caggcgccgc
     6241 gggcgcctgc agcacctctt ccaccatggt atcccatccg ccgcactgcg ggcatttgcc
     6301 caaaggtcgc ggcgccactt ttccgcattg ctggcataca tactggctga tggttttagg
     6361 cataacctga ttatactcaa tccacccgtt tggagaacgc gaacctggcg cgcggtttgc
     6421 gcctggagat tacccacact cagattggct gctgatttga ggttttgtac tttcagaaac
     6481 gacgaaagca aaacagcaac cgtcaaatca cgaaggtttt accggaaaga agttgcaatc
     6541 gcccccgcgg tttactagcg catggcaaca acgccaaccg ctggtaaaaa agcagcggtc
     6601 acggcgcccg cctcccctgg cgaaggctgc cttttacgcc aaaggatcag gccaggtgtt
     6661 tgacgtaggc gcgccggcgc ttatgctggc ggctgtcaag gtacttccac atggccagga
     6721 tatcctggtt attctccagc gtggcgttgg tttcgatata tcgaataccg tgtttcttga
     6781 aggtctgaaa gatctggtgc atcaacaggg cggtgatccc tctgccgcgg tactcatctc
     6841 tcacgcccac caggtacagg tcggcgcggt cgttcttgtg gagggcgcgc aaaaagtgga
     6901 tgaagccgaa cgggaagagg ttgcccttac tcttttgcag cgcgcgcgag aaagagggaa
     6961 aggtaatacc gaacgccacc agccgctcgt tctcgtccat taccaggggg atgaaatcag
     7021 ggttggcgaa gccgaaatag gtgttgacgt aactcttcac ctgcgcttca gtgaggggca
     7081 cggtgccgta caggtggcga tacgcctcgt tgatcacatc gaagatctgc ggggcgagcc
     7141 ccagcaggtc attcttttta ccctgaaaga ggtgcgtttt agtgcgctgg gcaattaatg
     7201 cggccgcgtt ggcaattttt tcttccagct gggtggagaa ggtgaactcg tgctcgatcc
     7261 aatccacatc cttggtgtac cccagttctt ccagccgctg cggataatag gggtagttgt
     7321 agatggtggc gagtgtggtc atttcgtcga agccttccac cagcataccc tcgcggtcga
     7381 gatcggtaaa acccaacgga ccatgcacgc cgagcttgcc ctcctgccgc gcccagttct
     7441 ccaccgtgtc catgagcgct ttcaccactt ccgcatcgtc gataaaatcg atccagccaa
     7501 aacgcatgta cttttcaccc catttttctt cgtgacgcgg cacaatcatg gcggcgacac
     7561 ggcccacagc ctggttctca cgataggcca tccagtagcg cgcgcgggcg tcttcaaacg
     7621 ccgggttctt atccgcgcgc agcgtgttca attcatcggc gaacaggttc ggaacgtaat
     7681 aagggctgcc cttgtaaagg gtgagagggt agcgtatgaa tttttttaga cccttgattg
     7741 tggtgacttc tctgatctct actgccattg gtgtttctcc tggttctaaa ttccagcatc
     7801 tgccggttgg tacacagtca gaattcctgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn ccgccccagg ggaagtagtt gaacacctct ccgcgctgtg ggttttgctc
     7981 caaccagtca accgcaccca cgggaaactt ggcggggtcg tacgcgttgg ccgttccgtc
     8041 gcgggtgaag gtcattccgg cggaaaaacc aatcaccact ataacaacac tcaacaccgg
     8101 ccaaaagata ccctttaagc gcaaatccac cttcagcaac ccggcgtcca acttctcaac
     8161 ccggcggcgt agaatttcgg gcaactccag ccctgagaga aaatccacgc aggcttttgc
     8221 cagcaaaggc gccgccacaa tggcaaacag cgggatgttg cgcgcgctat aaagcgacat
     8281 caccagccac gccaccgcgg ggaacaattg aaaagaggtt gtgtggcgct tccgcagggc
     8341 gagaacgacg atcaacagcg cgatgaagag cagcagcagc caggtgatgg gctcatgaaa
     8401 attgggagac tggtactcaa ccgtgtggtc caccaggtaa cggttggcaa cataacccac
     8461 gctggtgccc cagattccca ggccggccgg gttgagcagg gtggccagca gtgaaaaccc
     8521 gccgcccaac aggtaactct tccagaaacc cgcgggagga acctctctct cttcgctgcg
     8581 ggtgaaccat ctctcccaaa gcatgccgaa ggcgcacaat ccccaggtgg caaagccggc
     8641 gatgaacgcg ccgtgcaggt tcacccaggc gatcatcagc aacggcatca gccaccagcg
     8701 ccgggtgttt ccttgtttga tctggtagag cacgcccatc cacaaggcca gcaacaggaa
     8761 ggtgaagata tgggggcgtg ataaccaatg gatggaagaa gccagcagag agagtatgct
     8821 caccatggca gcggcgagca gggaaccgct ttgcgcgcgg gtctgccggt aaaccagcca
     8881 gaacgcggtg gcaatcacca gcgcggcgag caggacggcg ccatttatgc ccattaaacg
     8941 atgcacctgc gcgaacaata cctgagcgag ccattcgtgc ggggtgagcg gttcacccag
     9001 catggtgtgc gaaaaaacat cgcgcagggg aacgagatga ttgtcgagga tataaccgcc
     9061 gatggtgata tgcctgccta ggtcaccatc agcgttgacc atgcgcgggc cagacatcag
     9121 cacaccaaaa aagatgctga tccatagtat ggcgccaact gagggcagca agtacagcaa
     9181 aacagactcg atgcggtttt tcatttcgtt gaccccgggc taaggaaatt catcgtaagc
     9241 ataaaaacct caacaattcg cgcagaagcc attctgctca gaacccctgg tacacaaatg
     9301 gggcggtcgt atctgagaag aacgccagcg tcgccaccaa caaaagcagc gctgtcagca
     9361 gcacctgcaa ccagcgcggt tggttcagca tgaattcctc ggtgcccgcg cggtgctgca
     9421 attggtcaaa gagcagcgcg gcggcgagca caagcagcag gatcgggttg ggaagatcaa
     9481 attgcgacca gcctgagaca gggctccaac ccaccagcgt acgggcaagg ccgatgaaat
     9541 ccggcatgct ccagcgcagc aaaccctgcc agtactgcag ggcggcgggc agcggcatac
     9601 ggaagggcac ccaggccagc gacgcgaaca gaaaggtcac cgctgttccc agccgttggc
     9661 gccagagcgg gcgcttgttg acaggcacaa cagccttccc caacgagggc agccgttcca
     9721 gcaccaggta gagaccgtgc agggctcccc acaccagcaa actccaggag agaccgtgcc
     9781 acaaaccgct taccagcagc gtcaccatcg gcgggaggaa aatgttgaaa aaatggtctt
     9841 gctgatggaa ccgccgccgc agagaccagg ccagcgggta gtaaatataa tccctcagcc
     9901 agtttgaaag ggtgatgtgc cagcggttcc agaattcggt gaaatttcgc gcgtagtaag
     9961 gcaaattaaa attggccgag agctcgatgc caaaccacag gctgacacca cgcacgattg
    10021 ccgtataccc ggcgaaatc
//
LOCUS       DAOSPM010000067         9586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_7096_length_9586_cov_12.494072, whole genome shotgun sequence.
ACCESSION   DAOSPM010000067 DAOSPM010000000
VERSION     DAOSPM010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7096_length_9586_cov_12.494072"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /gene="mazG"
                     /locus_tag="PLW25_06715"
     CDS             <1..693
                     /gene="mazG"
                     /locus_tag="PLW25_06715"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOS54215.1"
                     /translation="SLRPYLLEETYEALEQLDKNNMEGLKEELGDLLLQIALHAQIAA
                     EANTFNITQVLQQINRKIVSRHPHVFGSVSVKNDRDVVQNWEKLKELERSAKGKEQHS
                     SLLDGIPQILPALAQAQSIQERAARVGFDWPDIEPVLAKVLEEMREVSEVQNERERAQ
                     ELGDLLFAVVNLVRWYGVDAESALRCTNQKFRRRFAYIESAAQMTGRELQNMTLEEMD
                     TYWEESKKLDEP"
     gene            752..2290
                     /gene="ftcD"
                     /locus_tag="PLW25_06720"
     CDS             752..2290
                     /gene="ftcD"
                     /locus_tag="PLW25_06720"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOS54216.1"
                     /translation="MMTKPLIECIPNFSEARRPEVIGAIIEAIQSVDGVRVLDQHSDL
                     DHNRTVITMVGSPSSIEEAAFQSIKTAAERIDLEQHQGAHPRIGAADVVPFVPISGVS
                     MSDCVELAKRLGKRVGDELSIPVYLYEEAATTPDRQNLENIRKGQYEGLKAEISTLAE
                     RKPDYGPARLGSAGATVIGARAPLIAFNIYLTTSDTEIAQKIAKAVRHSSGGLRYVKG
                     MGVLVEGLAQVSMNLTNFHKTPIFRVVEMVRREAQRYGTQIHHSELVGLIPQSALIDT
                     AVWYTQLDQFDPEQILESRLYSSQEDLTPAPNNPYAFIEDLASGNPTPGGGSAAAFSA
                     AEAAGLVAMVGRVTIGKKSYAAVEAQMREMIEKADDLRANLTSAVEEDAAAFNSLMQA
                     LRLPKANDEEKMRRENAMEEATLQAAMVPHRVAGWALEVLRLSREAVELGNLNAISDG
                     ASASYLAAAAIKSAGMNVKINRSALKNPSAADGLMADLAAYAQEMNEIEQHIARLLQQ
                     RAQF"
     gene            2314..2868
                     /gene="dcd"
                     /locus_tag="PLW25_06725"
     CDS             2314..2868
                     /gene="dcd"
                     /locus_tag="PLW25_06725"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOS54217.1"
                     /translation="MGIKADKWIKKMALEKKMIDPFVESQVREGVISYGLSSYGYDIR
                     VADEFKIFTNVNSAIVDPKNFDTRSMVDFQGEVCVIPPNSFALARTIEYFRIPRSVLT
                     ICVGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPAKIYANEGIAQVLFFEGDE
                     VCDISYADRKGKYQKQSSILLPKL"
     gene            2891..4147
                     /locus_tag="PLW25_06730"
     CDS             2891..4147
                     /locus_tag="PLW25_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54218.1"
                     /translation="MKKIPCSVLMKFTGITLWVLLAAQLLSGCSVAQSFMPTETPTST
                     ATPLPTATPLPTSTPTEVTFYLDAAVMPLNQQAPILLYHRFIPDWEAKDAITFTRLSE
                     FKNDLQKLYDSGFSLVSLKSWIDGTYLVPPGKKPLVITIDDFWFADQIYISEDGTPSP
                     YSGIGVLWRFAQEHPDFGFSAAGFSNMGDKYYGDRQIGDKFFYGDNQDAMWLKLGQTI
                     AWAIENGVEPYNHLLQHPMLSKTADKDIQWQIEENDRLTRYYLELAGRGDLIPRLGNM
                     IALPFGEWPATYSGIEIIKKYQNQEGKPVEAIFEAYLYADKELTPSAFSNDFNRYNLA
                     RLTASEIMTQWLLQQAPDLPDAQSCRLGPLAQNQADDQAVIQGLISAAIQSGSCFEGA
                     YHVGEQIFLARDGEVVPYSPPSIIAP"
     gene            4688..5809
                     /locus_tag="PLW25_06735"
     CDS             4688..5809
                     /locus_tag="PLW25_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54219.1"
                     /translation="MRKRLKKLERYNPFKDRTPVQRWNAAQYLMITLLSFAASISGTR
                     LFLEVTGFPRLESGEIHIAHVLWGGLLLFIGALLPLILANEWGLRLSALLTGLGIGLF
                     IDEVGKFITQTNDYFHPVAAPIVYVFFLLTVLLFAFIRNRRKSSSRADMYEILERFNE
                     VLDHDLSTEEYRGLLQRLDNVIAAAEPRSLVELAESMRDYLKTNRSRVVPDDPIFIER
                     IRAALQRFEKNWLVPKRFKKVVVLGLLIWGVWALVSPIGYLLITRDAAQLSAFVDQLI
                     SNRLVRNASGLNWFEARVLIEGGVGVLAILSAIMVLFKFEKRGVMLGIVDLLVTLTIV
                     NPLVFYFEQFSTLIVAAFQFILLILLIRYRKRLQRMENE"
     gene            5809..6357
                     /locus_tag="PLW25_06740"
     CDS             5809..6357
                     /locus_tag="PLW25_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HOS54220.1"
                     /translation="MEPGNLPYPESYWVVPGKLMAGEYPGKYDSERTRQRVVSLLRCG
                     LNAFIDLTRPADSFFPYLQVLKAEAEDFGLEVTWHNFPIDDFTAPPPKLMRQILDEID
                     AQIASGKTVYVHCFAGIGRTGTVVGCYLVRRGLTGSEALQRINELRKDTPTWWHRSPE
                     SQSQVDLIMDWKDGTNPAADQR"
     gene            6491..7072
                     /gene="rplI"
                     /locus_tag="PLW25_06745"
     CDS             6491..7072
                     /gene="rplI"
                     /locus_tag="PLW25_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOS54221.1"
                     /translation="MKVLLLKDVYKLGHAGDVKKVADGYGRNFLLPQGLAVLATAGAL
                     KTVEKIRERAAVERAKLNQEMSGVAELLQDLELQFFSKAGETGKLYGSITSQMIVDEI
                     HNKLAISLDRHQIEVAPIRTLGEHLATVRLTVDLNPKIKVLVNREGEAAKVSARKQKV
                     EEPIIEAAAVVEETITSAAEETMATEETEEKAE"
     gene            7159..8553
                     /locus_tag="PLW25_06750"
     CDS             7159..8553
                     /locus_tag="PLW25_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOS54222.1"
                     /translation="MTVGEYLRRIREEKQISLEQVSSGTNIRVYYLEAIEADNWEQLP
                     SQAQGRGFARLYAAYLGLSSRDVFDEVEQMARHELGGAPETEPALKQPAAILVERPQP
                     FGQKPGKKRSSLSATSRERNEEALSPVTAPPVSQRIFNEIGVELRRQREALGLSLDDV
                     ERLTKIREFYVFALENGRVDDLPSSVQGRGMLNNYASFLSLNADALQMRYAEALQQRR
                     LEKLEQERVEKKEGVAVMGKEPLTGWRRFLTPDLLVGGMVFFVLFSLVVWGAVKMINT
                     SIPRTEPTISSISEILVGETEDITALTDEPTTQLTEDTPEATQWINVPQTDMQATIAA
                     VEPGPIQVVVVAYQRAYMKITVDGNEKFSGRVIPGNVYAYTGTYKISLITGNAAALQV
                     YYNQQDLGVLGPLGQVAEIEFTRQEMMTPTPRFSPTPSLTPLPTLTALPTNTPMPTAT
                     VPTPTITPALTIKP"
     gene            8564..>9586
                     /locus_tag="PLW25_06755"
     CDS             8564..>9586
                     /locus_tag="PLW25_06755"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOS54223.1"
                     /translation="MKRNSYYLVSLGCAKNSVDSQSMAVLLDRDGYKAVEKPAQAEVL
                     IVNTCGFIEQARQESMEVLADLARSKKTGQLLIAAGCFSQLYRERLVEMVPGIDGLIG
                     TRRWMDIATYLHELRAHPGEPRYHLPENPTIGTDEQGVPRVSLQGASAYLKIADGCRR
                     PCAFCSIPLIKGLQVSRPLETILHEAELLQSHGIKELILIAQDTTDYGSDLGMKDGLV
                     RLLEQLLPRIPAIPWVRILYAYPGYVTDGLIDLMSSAHQILPYLDMPLQHASVPVLRA
                     MKRPSNMDWVRRTIEKMRARIPDLALRTTFISGYPGETDAHHQELLEFIREMTFDHLG
                     VFPFSFE"
BASE COUNT         2369 a         2376 c         2633 g         2208 t
ORIGIN      
        1 agtttgcgcc catacctgtt ggaagaaact tacgaagcgt tggaacagct tgataaaaac
       61 aacatggagg ggttgaagga agaactgggc gacctgctgc tgcaaattgc gctacatgcc
      121 caaatcgcag cagaagcaaa tactttcaat atcacgcaag tattgcaaca aatcaaccga
      181 aaaatcgttt caaggcaccc gcatgtattt ggttcagtga gcgtaaaaaa tgaccgggat
      241 gtggtacaga actgggaaaa gctcaaggaa cttgagcgtt ctgctaaagg caaagagcag
      301 cattccagcc ttctggatgg catcccgcag atcctgcccg cgctggcgca ggcgcaatcc
      361 attcaggaac gcgctgcccg ggtgggcttt gactggccgg acattgagcc ggtgcttgcc
      421 aaggtgctcg aagaaatgcg cgaggtcagc gaagtgcaga atgagcggga acgcgcgcag
      481 gaactggggg atttgctctt cgctgtggtg aatctggtgc gctggtatgg tgtggacgcg
      541 gaatctgcct tgcgttgcac caaccagaaa ttccgccggc gctttgcata catcgaatca
      601 gcggcacaga tgactggtcg cgagttgcaa aacatgaccc tggaagagat ggatacgtat
      661 tgggaagagt ccaagaagct ggatgagccc tgagcaggaa atatggcaga aaatgcctgg
      721 gttataattt gacaaacata aaacgagggc aatgatgaca aaacctttga tcgaatgcat
      781 ccctaacttt tcagaagccc gccggcctga agtgattggc gccatcatcg aggccatcca
      841 gtctgttgac ggtgtgcggg ttttagacca gcactctgac ctggaccata accgcacggt
      901 catcaccatg gtcggcagtc catcttcgat tgaagaagca gcctttcagt cgataaaaac
      961 agccgcggaa cgcatcgacc ttgagcagca tcaaggagca cacccgcgca ttggggcggc
     1021 agatgtcgtg ccatttgtcc ccatctctgg ggtaagcatg agcgattgtg tagaactggc
     1081 aaaacgcctg ggtaaacggg tgggagatga gctatcaata cctgtttact tatatgaaga
     1141 agccgcaacc acacctgacc ggcaaaacct tgaaaacatc cgtaaggggc aatatgaagg
     1201 attgaaagcc gaaattagca cactggcgga gcggaaaccg gattatggtc ctgccaggct
     1261 gggcagcgca ggcgcaacgg tgattggcgc cagggcgcct ttgatcgctt ttaatattta
     1321 cctcactacc tctgatactg agatcgcgca aaagattgcg aaagcggtgc ggcactcatc
     1381 aggggggctg cgttacgtga aaggtatggg cgtgctggtg gaagggctgg cgcaggtttc
     1441 gatgaacctt acgaattttc ataaaacgcc catcttcagg gtggttgaaa tggtgcgacg
     1501 tgaagcacag cgttacggca cgcaaatcca tcacagcgaa ctggttggtc tcattccaca
     1561 gtccgcgctc atcgatacag cggtgtggta tacccagtta gatcagttcg acccagagca
     1621 gattcttgaa agccgcctgt attcctcgca agaggacctc acgcccgcgc cgaataaccc
     1681 gtatgctttt attgaagacc tggcgagtgg caaccctacc ccgggtggcg gttcagccgc
     1741 cgcgttcagc gctgctgaag cggccgggtt ggtggcgatg gttgggcgag tgaccatcgg
     1801 caaaaaatct tacgccgcgg tggaagcaca aatgcgcgag atgatcgaaa aagcggatga
     1861 tttgcgggca aacctgacca gcgcggttga agaggacgcc gccgctttta acagcttgat
     1921 gcaggcgctg cgtttgccga aggcgaacga tgaagaaaaa atgcggcgtg aaaacgcgat
     1981 ggaggaagcc acactgcagg ctgcaatggt gcctcaccgt gtggctggtt gggcgctgga
     2041 agtgctgcgc ctttctcgcg aagcggtgga gttgggcaac ctcaacgcta tttcggatgg
     2101 ggcttctgcc tcctacctgg ctgccgcggc gattaaaagc gccggcatga acgtgaagat
     2161 caaccgcagc gccctgaaaa acccgtccgc agccgatgga ttaatggctg atcttgccgc
     2221 gtatgcacag gagatgaatg agatcgagca gcacatcgct cggcttttac agcaacgggc
     2281 gcagttttag gttagaaaca caggagtttt catatgggca tcaaggctga caaatggatt
     2341 aaaaagatgg cgttggaaaa gaagatgatt gaccctttcg tggaaagcca ggtgcgcgaa
     2401 ggagtcattt cgtacgggtt atcttcatac gggtatgata tccgcgttgc cgatgaattt
     2461 aaaatattta ccaacgtgaa ctctgccatt gtagatccca agaacttcga tacgcgctct
     2521 atggtggatt ttcagggcga ggtatgcgtg atcccgccca attctttcgc gctggcacgt
     2581 acgattgaat atttccgcat cccgcgctcg gttctcacca tctgcgtggg aaaaagcact
     2641 tacgcgcgct gcggaatcat cgtcaatgtg acgccctttg aacccgagtg ggagggctat
     2701 gtgacgctgg agatctccaa taccacgccg ctgcctgcca agatctacgc caacgagggg
     2761 atcgctcagg tgttgttctt tgagggagac gaggtttgcg atatttctta cgcagaccgc
     2821 aagggaaagt accaaaaaca atccagcatt ctcttgccaa agctctgaaa ccagatatac
     2881 ttggtcggat atgaaaaaga taccttgtag tgttttgatg aagttcactg ggataacctt
     2941 atgggtgtta ttggctgccc agctgctttc aggctgctca gtagcgcaat cctttatgcc
     3001 cactgaaaca cccaccagta cagccacgcc gttgcccact gccacgccgc tgcccacttc
     3061 cacgccgacc gaagtgactt tctaccttga tgccgcggtg atgccgttga accaacaggc
     3121 gccgatattg ctctatcatc gctttatccc ggattgggag gccaaggatg ctattacttt
     3181 tacgcggctt tccgagttta agaatgacct gcaaaagctt tatgacagcg gattttcgct
     3241 ggtttccctt aagagctgga tagacggcac ttatcttgtg ccgcccggta aaaaacccct
     3301 ggtcatcaca atagatgact tctggtttgc ggaccaaatc tacatcagcg aggatggcac
     3361 gccttcccca tactcgggaa taggcgtact atggcggttc gcgcaagaac atcctgattt
     3421 tgggtttagc gcagccggtt tttcaaatat gggggataag tattatggtg accggcaaat
     3481 tggagacaag ttcttttatg gagataatca agacgccatg tggttaaaac ttgggcaaac
     3541 catcgcctgg gcgatcgaga atggcgttga accatataac cacctgctcc aacacccaat
     3601 gctgagcaag acagccgata aagatattca atggcaaatt gaggagaacg acaggcttac
     3661 ccgttactac ctggaactcg cgggcagagg cgatctgatc ccacgattgg gaaatatgat
     3721 tgcccttccc tttggggagt ggccagccac ctacagcggc attgagatca tcaaaaagta
     3781 tcaaaaccag gaaggcaagc ctgttgaagc gatatttgaa gcctatctgt atgccgataa
     3841 agaactcaca ccatcagcct tttcaaatga ttttaatcgt tataacctgg cgcggttaac
     3901 cgcatctgaa attatgacgc aatggttatt gcaacaggcg cctgatttgc ccgatgcgca
     3961 gtcttgccgg cttggcccgc tggcgcagaa ccaggcggac gaccaggcgg tgatccaggg
     4021 tttaatttcc gcagcgatcc agagcggcag ttgttttgaa ggagcgtacc atgtagggga
     4081 gcagatcttt ctcgcccgtg atggcgaagt tgttccttac tcgccccctt caattattgc
     4141 tccgtaagaa ataaggcttg ccaaatgaac atgaaagccg ctgaattttc agcggcgtaa
     4201 ttttgtataa ttacgatgcc gctgttttct ggagggagga tgtttcgaat tcaatacgct
     4261 atcgagtata catgcgagga ggcccccttc acccctgagc gtaatgtcat tacctgggtg
     4321 tggtgcacgc ttcgcggcat ggcgtatgag gcgttcgatt tgtgccaggc actcgggcgt
     4381 cgacaggata cgcataagag cgtagtacgt ccactcggtt cgcttttcaa tgatgggggt
     4441 ttggtcgtga agcctcctaa gccttgtgag ttggttgcca accctgcgag ccgcaaacag
     4501 gatttcaaca caatagactg ggagtatcga gagacttcac gaagagcaaa cgcctatata
     4561 cggtcggggg gcattgtcag cctgctgttg acaatcttct ccagtatgga gaacgctcag
     4621 ttccgctcga ttcggcattc ttcgtgttca tcggcggtaa cagaaacagg cattgacagg
     4681 ttccatcatg cgtaaaagac tgaaaaaact ggagcggtat aacccgttca aagaccggac
     4741 gcccgttcag cgctggaacg ccgcccagta cctgatgatc accctgttga gttttgctgc
     4801 ttcaatcagc ggcaccaggc tctttctgga ggtgacggga tttccaaggc ttgaaagcgg
     4861 tgagattcac attgctcatg tattatgggg agggttgctg ctattcattg gcgcgctgct
     4921 gccgttgata ttggccaacg agtgggggct acgcctttca gcgttgttga ccgggttggg
     4981 aatcgggctc tttattgatg aagtgggaaa attcatcacc cagaccaatg attattttca
     5041 ccctgtggcc gcccccatcg tgtatgtctt ttttttgctc accgttttgt tgttcgcatt
     5101 cattcgcaac cggcgcaaat cttccagcag ggcagatatg tacgaaattc tggagcggtt
     5161 caatgaagtg ctggaccatg atctttctac agaagaatat cgcggcttgc tgcaacgttt
     5221 agataatgtg attgcagccg ctgagcccag gtcattggtg gagctggcag agagtatgag
     5281 agattacctc aagacaaacc gctccagggt agtgcctgat gacccgatat ttatcgagcg
     5341 catacgcgct gcgctgcagc gattcgaaaa gaactggctt gtgccaaaac gttttaaaaa
     5401 agtggtggtg cttggccttt taatttgggg agtttgggca ttagtgtcgc ctatcggcta
     5461 tctgctgatc accagagacg ccgcgcaatt atcagcgttt gtagaccagc ttatttctaa
     5521 ccggctggtg cgcaacgcca gtggattaaa ctggtttgaa gccagggtgc tcatcgaagg
     5581 aggggtgggt gtactggcca tactgagcgc cattatggtc ttgtttaaat ttgagaaaag
     5641 gggcgtaatg ctgggaattg ttgatctctt ggtgacgctc actattgtca acccgttggt
     5701 gttctacttt gaacagtttt ccaccttgat tgtggcagca tttcaattta tcctcttaat
     5761 attgctcatt cgataccgca agcgccttca aagaatggag aatgaataat ggagcccggt
     5821 aaccttccct atcctgaatc ctactgggtt gtacccggta agctgatggc tggtgagtac
     5881 ccaggtaaat atgacagcga acgcaccagg cagcgggttg tttccctgct gcgctgcggg
     5941 ctgaacgcgt ttattgacct gaccagaccc gcagattctt tctttcctta ccttcaagta
     6001 ttgaaggcag aagcagagga ttttggattg gaggtcacct ggcataattt tcccatcgac
     6061 gattttaccg ctccgccgcc aaagttaatg cggcagattc tggatgagat agatgcgcaa
     6121 atcgcttcag gaaagacggt gtacgtgcat tgcttcgctg gaatcggcag gacaggaacg
     6181 gtggtgggct gttatttggt tcgcaggggt ttaaccgggt ctgaggcgct gcagcgaatt
     6241 aatgagctgc gcaaggatac accaacctgg tggcaccgca gccccgaaag ccagtcgcag
     6301 gtagatttga tcatggattg gaaagatggg acgaatccgg ctgccgacca acgatgatcg
     6361 atgaatcagc agccaacatg gcgtctatct tggtgtctat cttggttgac caacctggcg
     6421 cgttgcggta aaatccctgt tcacatgcgt tgtacccttc gcatgaattc tgctttgaaa
     6481 gggcttgaac atgaaagtac tgcttttaaa agatgtatac aaattgggac atgctgggga
     6541 tgtcaagaag gtagcggatg gatacggccg caacttcctt ctcccacagg gtctcgcggt
     6601 gcttgccacc gccggagcgc tcaagaccgt tgagaaaatc cgtgaaaggg cagctgttga
     6661 gcgcgccaaa ctgaaccagg agatgagcgg agtggctgag ctccttcagg acctggaatt
     6721 gcaattcttc agcaaagcgg gtgagactgg caagctttac ggttccatta ccagccagat
     6781 gatcgtggat gagatccaca ataaacttgc catctcattg gacagacacc aaattgaagt
     6841 tgcccccatc cgcacattgg gtgaacacct ggcgacagta cgtttgaccg ttgacctgaa
     6901 cccgaagatc aaagtgctgg tcaaccgcga gggagaagct gccaaggttt ctgccaggaa
     6961 acagaaggtt gaggaaccca tcattgaagc cgccgcggtt gtggaagaaa caatcacttc
     7021 cgcagccgaa gaaacaatgg caaccgaaga aacagaagaa aaagctgaat aaggcgtttt
     7081 acctggattg cccaaatgac cggccaaatc agccaggggc tgaggccggt ctttcttaaa
     7141 atcggttaag attaagacat gacagttggt gaatacctgc gccgtataag agaagaaaag
     7201 caaatctcgc tggagcaagt ttccagtggg acaaatatcc gcgtctacta tctcgaagcc
     7261 atagaagctg ataactggga acaattacca tctcaagccc agggaagggg atttgcccgt
     7321 ttgtacgcgg cttacctggg attgagttct cgggatgtgt ttgacgaggt tgaacagatg
     7381 gcccggcatg aactcggggg ggcgcctgaa accgaacccg cgcttaagca gcccgctgcc
     7441 attttggtcg aaagaccaca accttttggc cagaaaccag gcaaaaagcg gtcatcgtta
     7501 agcgccacct cccgggaaag aaatgaagaa gccttgtcac ctgtgacggc tccccctgtt
     7561 tctcagcgca ttttcaatga aattggtgtg gaacttcggc ggcaacgcga agcactgggc
     7621 ttatctctgg acgatgtaga acggttgaca aaaattcgcg aattctatgt attcgcgttg
     7681 gagaacgggc gggtggatga cctgccttcc agcgtgcaag gcaggggaat gctgaacaat
     7741 tacgcgtctt ttcttagcct gaacgctgat gcattgcaaa tgcgttacgc cgaagctttg
     7801 cagcaacgcc gccttgaaaa gctggagcaa gaaagagtag aaaaaaaaga aggggtggcg
     7861 gtgatgggca aggaaccgct taccggctgg cgcagatttt taacacctga cctgctggtt
     7921 ggcggtatgg tcttcttcgt tctgttttcg ctggtcgttt ggggtgcggt caagatgatc
     7981 aacaccagca tcccgcgtac agagccaacc atcagttcaa tttcggagat tctagtgggc
     8041 gagacggaag acatcaccgc gctgactgat gagcccacca cccagttgac tgaagacaca
     8101 cctgaagcaa cccagtggat caatgttccg cagacagata tgcaggcgac catcgccgcg
     8161 gtcgaaccag ggccaatcca ggtggtcgtg gtggcgtatc agcgggctta tatgaaaatc
     8221 accgtggatg gcaatgaaaa gttcagcggt cgtgtcattc cggggaacgt ttacgcttac
     8281 acgggtacct acaagatttc gcttatcacc gggaacgcag ctgccttgca ggtgtattac
     8341 aaccaacagg acctcggcgt gctgggcccg ttgggacagg tggccgaaat tgaatttacc
     8401 cgccaggaaa tgatgacgcc cacgccgcgc ttttcaccaa cgccctcttt aacgccgctt
     8461 cccaccctga ccgccttacc gaccaatacc cccatgccta ccgctaccgt acccacgcct
     8521 accatcaccc cggcgctcac cataaaaccc tgaggaaaac tggatgaaga gaaattctta
     8581 ttacctggtt tcactgggct gtgccaagaa ctcggtggat tcgcaatcca tggccgtctt
     8641 gcttgaccgg gatggatata aagcggttga aaaacctgcc caggcggagg tgttgattgt
     8701 gaacacctgc ggatttattg aacaggcgcg ccaggaatcg atggaagtgc tggctgacct
     8761 ggcgcggagc aaaaagactg gccagttgct catcgctgcc ggctgtttca gccagttata
     8821 ccgcgagaga ctggtggaga tggtgcctgg catcgatgga ttgattggca cgcgccgttg
     8881 gatggatatc gctacatacc tgcatgagct gcgcgcccat cctggagaac cgcgctacca
     8941 tcttcccgaa aatcccacga ttggaacaga tgaacaggga gtacccaggg tttcactgca
     9001 aggcgccagc gcctacctta aaattgcgga tggctgccgc cgaccctgcg ctttttgctc
     9061 catccctctg atcaaaggac tacaggtgag ccgaccgctg gaaaccatcc tgcatgaagc
     9121 ggaacttctt caatctcatg gcatcaaaga gctgatcctc atcgcgcagg ataccactga
     9181 ttatggctct gacctgggga tgaaagatgg attggttcgg ctgctcgaac aacttctgcc
     9241 gcgtatcccc gcaatcccct gggtgcgcat tctttacgcc tatcccgggt atgtgacgga
     9301 tgggctgatt gacctgatga gcagcgcgca ccagatactg ccttacctgg atatgccgct
     9361 gcagcacgct tcagtgccag tattgagggc gatgaaacgc cccagcaata tggactgggt
     9421 gcgccggacc atcgaaaaaa tgcgcgcgcg catccctgac ctggcgctgc gcactacttt
     9481 catctcaggg taccctggtg aaactgacgc acatcaccaa gagctgttgg aatttatccg
     9541 cgaaatgact tttgaccacc tgggtgtttt ccccttctcc tttgag
//
LOCUS       DAOSPM010000068         9392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_7276_length_9392_cov_12.562172, whole genome shotgun sequence.
ACCESSION   DAOSPM010000068 DAOSPM010000000
VERSION     DAOSPM010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7276_length_9392_cov_12.562172"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..76)
                     /locus_tag="PLW25_06760"
     tRNA            complement(3..76)
                     /locus_tag="PLW25_06760"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Arg,seq:cct)
     gene            139..1389
                     /locus_tag="PLW25_06765"
     CDS             139..1389
                     /locus_tag="PLW25_06765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOS54224.1"
                     /translation="MSLSKENTQFELEILDFAYGGNAFSRLPDGRPVFVPFTMPGERV
                     RVRVTTAFKDYCTAQLEEVLKPAPGRVAPRCQHFTVCGGCHYQHLPYQQQLEVKQRIV
                     ADQLRRIGKITAPAVNQIIPSADEWQYRSTMQFHISKNGRPGFRAGDGKTVIEVNECY
                     LPIKGIDKLWPRLDIDAQSGIRRVMLRAGSDSELLAALEGESNLPPEFNLDFPLSVHY
                     LSSDRDYLLAGDAHNLMTVNGVNFMVSPRSFFQVNLGQACKMVNYVLANVDLNSAVSA
                     LDLYSGVGLFSAFLAPRVKELIAVESSASACDDFAANLDFYDNVSLYVGGAEQVLPGL
                     RIAPDLVIVDPPRAGLMFKVVDYLVNSPVTQLVYVSCDPATLARDCWRLTQGGFTIRS
                     IQPFDFFPQTYHVECVVFLDKKRG"
     gene            1595..2554
                     /locus_tag="PLW25_06770"
     CDS             1595..2554
                     /locus_tag="PLW25_06770"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOS54225.1"
                     /translation="MKKVIKLTTAFLVLSFLLSSCNLPFFRGDAAEENNPPNLTLTAL
                     FDTSMNIPPSMTPALPTSVEVIQTTPAIVLPTLAGSDSPTPNPIPTNTSAPPTAEVRQ
                     RSGKLMKAAYLSTPPVMDGTYAEWVDKTNKYAIPYVVWQPSNWKNMEDLEGAFAAGWD
                     DKYLYISVKVTDDVFAQNKSGESLYLGDSVEVMIDANLDGDFYVNYLNDDDFQFGISP
                     GNPAKGIDPEVYLWYPSALKGSKSQVLVSSQFETSPIYRVEVGIPWSMLGVSPYNGLH
                     LGFVVSVNDNDKPSENLQQSMISTAQHRNFLDPTTWGELMLVK"
     gene            complement(2595..3428)
                     /gene="trxA"
                     /locus_tag="PLW25_06775"
     CDS             complement(2595..3428)
                     /gene="trxA"
                     /locus_tag="PLW25_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOS54226.1"
                     /translation="MTSEHIISVTESSFEYDVIAYSQNIPVVVDFWADWCKPCRVLTP
                     MLERFAEQSQGEFRLAKVDVDANPNLALRFSVRSIPTVMAFSQGRKVSDFTGLVPEHV
                     VREFLARILPPRPSDLLAQKGNSLLHAGELKEAMEAFMQALDLDENHSPSLLGMIKIN
                     LLEGNSAAARSLYQNFPASPEFNAAEKLVPLIKALQDIDDDQLPQENDLDAVFVNSIR
                     LVKRRNTLAGIDGLLEVIRQDKRYRKDLARSVLLALLELLDPEAELTRKYRAELASIL
                     F"
     gene            complement(3472..3888)
                     /locus_tag="PLW25_06780"
     CDS             complement(3472..3888)
                     /locus_tag="PLW25_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54227.1"
                     /translation="MAAEPLFKGLISDEFDHPVDTAIVGSEPCYVIDDAGFKRHVPSR
                     PIDLEILRSLFKQVEGNEDIIAAQAAKMTGQDDIFSHAILEASLKNIDKQYEFLLDHG
                     IPEETRAYLGLMGFRVVLDIRGNILKLEYPSAPSSE"
     gene            complement(3968..5023)
                     /locus_tag="PLW25_06785"
     CDS             complement(3968..5023)
                     /locus_tag="PLW25_06785"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOS54228.1"
                     /translation="MAEALDLRNMSRREFLKLSSASILSLLAIHSTTYAYAQTRSLQD
                     TAAQLGRITTNKVDMFPTPSAEGKPLKTLWKDLVLPITRMEVSSSEPSHNRVWYQLDG
                     QGYVHSGSVQPVKISLNETRQDIPVKGLLGEVTVPFTDAIWNPLLKNIVAYRLYYSST
                     HWITGILQDARGDDWYEVQEDFYRFKYYVNPAHLRVIAPAEVEPISPEVNAQDKRLEV
                     RLKEQIVVAYEGDAPVQMLRCSSGTEYRNGYLTPTGSFRTDYKRPSRHMVNGSLASSW
                     GYDLPGVPWICYFTEEGVAFHGTYWHNDFGRPRSHGCINLFSEAARWLYRWTLPVVPY
                     EEQLASKVGGTRVEISI"
     gene            complement(5025..6185)
                     /locus_tag="PLW25_06790"
     CDS             complement(5025..6185)
                     /locus_tag="PLW25_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOS54229.1"
                     /translation="MNKQDFPAYRQAILNAQAALKNRDFAEVRQWAHQAAQIAPEREE
                     PWLLLAAASPPAVSLQYLQKALAINPTSEWATKGMRWALNRLARTSLEETPAAKPQPQ
                     SPAADETQPIRVRAAKPDGAQPASPRPIAAAPREKGELPGQPQPAKQAVTPGTRKPVL
                     RRKTQRKWLIFLLVAVLIALAAFVIWAIVPNWIALARSSAAPIPADALVKPSLTPTAT
                     ATPTATPTATPTFTPTSTFTPTATNTPLPTDTPWPTYPPAAQEPVAIRTDNEGHWIDI
                     NLSSQMLYAFDGDEMVASFLVSTGTWLHPTVTGDYQIYVKYRYTDMRGDDYYLPDVPY
                     TMYFYRGYGIHGTYWHNNFGTPMSHGCVNMRTSDAAWLYDFADVGTLVSVHY"
     gene            complement(6182..7042)
                     /locus_tag="PLW25_06795"
     CDS             complement(6182..7042)
                     /locus_tag="PLW25_06795"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOS54230.1"
                     /translation="MSDDSLFDHVEPPFEPLPEEEESLLPESEEEEQDSHQPFRIWSS
                     IQSIVVTAFLLASLFTLFTPNNLFSGEMFDRIFQAWQANPRISAPVQVNTPATPALPI
                     GIVSGHYKNDSGALCPDGLTEEQVNLKIASLVQQKLMAEGYTVDLLEEFDDRLSQYKA
                     LALVSIHADTCDFISNEATGFKVAAAIHSVYPDKATRLTLCMADRYAQTTGLTYHPNA
                     ASEDMTYYHAFDEIHSTTTAVIIETGFLNLDRQILTENADLVAQGIVNGLLCFIRNEN
                     VTLVQPTPVP"
     gene            7156..7635
                     /locus_tag="PLW25_06800"
     CDS             7156..7635
                     /locus_tag="PLW25_06800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOS54231.1"
                     /translation="MFTRNDLRQVMRRWPSGVAILTTSDGLTTHGLSVNSFTSVSVDP
                     EMVTVTLANTTRAKRIVDRTGLFAINLLPETQAELSDVFAGRIPEHADRFAGVETTPG
                     LTQIPLLKAASAHVECRVVHTYEMPHSTLYVAEVLRAEKNADAPPLVYFNREYHRIQ"
     gene            7635..8204
                     /gene="amrA"
                     /locus_tag="PLW25_06805"
     CDS             7635..8204
                     /gene="amrA"
                     /locus_tag="PLW25_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOS54232.1"
                     /translation="MTNPLTEEEKHYLLQLARRTIEQALKQAPEPEIDLGMLSAALKE
                     PGASFVTLTIRRNLRGCIGSLEARQALAFDVREHARQAAFEDYRFPPLSKAELPATSI
                     EISRLTAPLDLEYQQPSDLPRILRPGIDGVILQDGYRRATFLPQVWEQLPNVDDFLSH
                     LCVKMGSPADLWKRKTLQVKTYSVEEFHE"
     gene            complement(8238..8504)
                     /locus_tag="PLW25_06810"
     CDS             complement(8238..8504)
                     /locus_tag="PLW25_06810"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOS54233.1"
                     /translation="MKMIIAIVNDDDSEMVTSALTHGDYRVTTIASTGGFLRRGLSTL
                     LCVAEDEKLENALALIRACFPETTDPSQKRCRIFVLNVVETHHF"
     gene            complement(8507..8854)
                     /locus_tag="PLW25_06815"
     CDS             complement(8507..8854)
                     /locus_tag="PLW25_06815"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOS54234.1"
                     /translation="MSEQPASTPNTMLMAVVQAQDADLVENILNDMNISLIRLPSTGA
                     FLGRRNATLIIGIPQEQQPQILNALHDNCRQRIEYIAVPLESAPLPLPAPTPVTVGGA
                     TVFALEIEHYEEL"
     gene            complement(8877..>9392)
                     /locus_tag="PLW25_06820"
     CDS             complement(8877..>9392)
                     /locus_tag="PLW25_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54235.1"
                     /translation="LMACYAGFLSEKDSRRITSRAKVKSYLTDHGLAVFPVSEPAPGL
                     LLPSYLAAMVIDGLVRYGKFDQADQLFQNHFLKDLGKGPSDQGSRSRCSTSRITELAP
                     TALFLRLHGLMKLSNRELVIRHYDGKQPPVTVQYNQLELLLKHHLTEIHLQSGEVLYL
                     NQPGTSRITLE"
BASE COUNT         2115 a         2408 c         2645 g         2224 t
ORIGIN      
        1 tgtgccccgg gagggactcg aactctcaac ctaagcgtta ggagtgcttt gctctgtcct
       61 attgagctac cggggcgaca atgtgattat atcattgaga aaaccgctct tcaagagaaa
      121 gagagggatc tttctgttat gagcctatca aaagaaaaca cccagtttga actggaaata
      181 cttgacttcg cctatggcgg gaacgctttt tcccgcctgc ctgatggcag accggtcttt
      241 gtgcctttca ccatgcctgg cgagcgggtg cgagtgcgcg tgacgacggc atttaaggat
      301 tattgcaccg ctcaactgga ggaagtgctt aagcctgcgc ccgggcgtgt cgctccccgc
      361 tgccagcatt ttaccgtctg cggcggatgc cattaccagc accttcccta ccagcaacag
      421 cttgaggtca aacagaggat cgtggcagac cagctgcggc gtatcggtaa gatcaccgca
      481 ccagcggtta accagatcat cccttccgcc gatgagtggc aataccgcag caccatgcag
      541 ttccatattt cgaagaacgg caggcctggt ttccgcgccg gtgatggcaa gactgtgatc
      601 gaggtgaacg aatgttattt gcccattaag ggtattgata aactttggcc gaggttggat
      661 atcgacgcgc aatcaggcat ccgtcgtgta atgctgcgcg ccggttcaga tagcgagctg
      721 ttggcagccc tggaaggcga aagcaacctg ccgcctgaat tcaacctcga tttcccactc
      781 tccgtgcatt acttgagcag cgatcgagat tacctgttgg ccggagacgc gcacaacctg
      841 atgacagtga atggggtgaa cttcatggtt tcgccgcgtt ctttcttcca ggtgaacctg
      901 gggcaggctt gcaagatggt gaattatgtg cttgccaatg ttgacctcaa ttctgcggtt
      961 agcgctcttg acctctacag cggggtcggc ctcttctctg cctttctcgc cccacgggta
     1021 aaagaactca tcgccgttga atcctctgca agtgcctgtg acgatttcgc cgccaacctc
     1081 gacttttacg acaacgtctc actttatgtg ggtggcgcag agcaggtttt gcctgggctg
     1141 cgaatagcgc ctgacctggt gattgtggac ccaccacgcg ctggcttgat gtttaaggta
     1201 gtggattacc tggtcaacag tccggtcacg cagctggtgt acgtctcctg cgacccggca
     1261 acgctggcgc gtgattgctg gcgcctgacg caaggcggct tcaccatccg ctccattcag
     1321 ccatttgact ttttcccgca gacctatcac gttgagtgcg tggtcttcct tgataaaaag
     1381 agaggatgaa gaggtggctg ttttccgtac ggtagagtgt tttttgaacg acgtctctct
     1441 gttccctggt gttcagcggt acagcgaaaa gactgccaca cgtacgggcg gcggttgcag
     1501 cgactttgac agggtttcaa aaaaatgtgt gataattgcc acgaaaagga ctggtttgac
     1561 ttgattacca ggcgtctttc tacaggagca caaaatgaaa aaagtgatta agttgactac
     1621 tgctttcctc gtgttaagtt ttctgctttc ttcatgcaac ctgcctttct tccgcggaga
     1681 cgcggcagaa gaaaacaacc cgccgaacct gaccctgacc gcgttatttg ataccagcat
     1741 gaacatcccg ccatcaatga cgccggcgct accgacctct gtggaagtta ttcaaacgac
     1801 tccggctata gttcttccca cgctggcagg cagtgattcc cccactccca atcccattcc
     1861 taccaatact tccgcgccgc ccaccgccga ggtgcgccag cgcagcggta agttgatgaa
     1921 agccgcgtac ctctcaaccc ctccggtgat ggacggaact tacgcggaat gggtggacaa
     1981 gactaataaa tacgccatcc cctacgtggt gtggcagccc tcgaactgga agaacatgga
     2041 agacctcgaa ggcgcctttg ccgcggggtg ggatgacaag tacctgtaca tttctgtaaa
     2101 agttacggat gatgtattcg cgcagaataa aagcggagaa tccctctacc tgggtgattc
     2161 agtggaggtg atgatcgacg caaacctgga tggcgatttt tatgtgaatt acctcaatga
     2221 cgatgatttc cagtttggca tttcgcctgg caacccggcg aagggtatcg accctgaagt
     2281 gtacttgtgg tacccttcgg cactcaaggg cagtaaatcg caggtgctgg tgtcttcgca
     2341 attcgaaacc agccccatct accgggttga ggtgggtatt ccctggtcca tgctgggcgt
     2401 aagcccctac aacggtttac acctgggttt tgttgtgtcg gtgaatgata acgataaacc
     2461 ctcggaaaat cttcagcaat cgatgatttc caccgcgcaa catcggaatt tccttgatcc
     2521 caccacctgg ggagaactga tgctcgtcaa gtaaacgggc gaataaaaaa ctggctgccg
     2581 acaggcagcc agttttaaaa gaggatagag gccagttcag cccggtactt gcgtgtgagc
     2641 tcggcttcag ggtcaagcag ttccagcaac gcgagcagta cgctgcgggc gaggtcctta
     2701 cgatagcgct tatcctgacg tatcacttcg agcaggccat caatgccggc gagggtattt
     2761 ctgcgcttga ccaggcgtat tgagttgaca aagaccgcgt cgaggtcatt ctcctgcggc
     2821 agctggtcat catcaatgtc ctgcagcgct tttatcagcg gcacaagttt ttcggcggcg
     2881 ttgaattcag ggctggcggg gaaattttga tagagcgagc gcgcggcggc gctgttaccc
     2941 tccagcagat tgattttaat catccctaaa aggcttgggg agtggttttc atccaaatcc
     3001 agcgcctgca taaaggcttc catggcttct ttcagctcgc cagcgtgaag caggctgttg
     3061 cctttttgcg ccagcaggtc agatggccga ggtggaagga tgcgggcaag aaactcgcgc
     3121 accacatgct cggggaccag accggtaaag tcagaaacct tgcgcccctg gctgaatgcc
     3181 ataacagtgg ggattgagcg cacactgaag cgcagcgcca agttggggtt ggcgtctacg
     3241 tctaccttgg ccaacctgaa ctctccctgt gactgctcag cgaaacgctc gagcatgggg
     3301 gtgagcaccc ggcagggctt gcaccaatcc gcccagaaat ccaccacaac cgggatgttc
     3361 tgcgagtagg cgatgacatc gtactcgaaa ctggattcag ttacggatat gatgtgctca
     3421 gaggtcatgc catctccatg gtgtcgtata caaatcctct ccctctgcgc cttactccga
     3481 agaaggagct gaaggatatt cgagtttgag gatatttccg cgaatatcca gtaccacgcg
     3541 aaaccccatg agccccaggt atgccctggt ttcttcaggg atgccgtgat ccaacaagaa
     3601 ttcgtattgc ttgtcgatat tctttaaaga tgcttcaagg atggcgtgag aaaaaatgtc
     3661 atcctgcccg gtcattttcg ctgcctgcgc agcgatgata tcttcgttgc cttcgacctg
     3721 tttgaataaa gagcgcagga tttccaggtc gatgggtcgg gatggcacgt ggcgcttaaa
     3781 cccggcatca tcaatgacgt agcaaggctc gctccctaca atcgcggtgt cgacggggtg
     3841 atcaaattca tcgctaatca atcctttgaa caatggttca gcagccatca tcatctccac
     3901 cctaattata aagcatagtg atgacgtgga acaccggtga gcaatagaag agcggctgag
     3961 gagtttttta aatgctgatt tcgacccgag taccgccaac tttactggca agctgttctt
     4021 cataaggcac aacgggcaag gtccagcggt aaagccacct ggcggcttct gaaaacagat
     4081 tgatacagcc atggctgcgc gggcgcccaa aatcgttatg ccagtaagtg ccgtgaaacg
     4141 ccacaccttc ttcagtgaag taacaaatcc agggtacacc tggcaggtca tacccccatg
     4201 aagaagccag gctgccgttg accatgtgcc ttgagggacg tttgtaatcc gtgcggaagc
     4261 taccggtggg cgtgaggtag ccattgcgat attcggtgcc actcgaacag cgtaacatct
     4321 gcacgggtgc atctccttcg taagccacta caatctgttc tttcaagcgc acttccaggc
     4381 gcttatcctg agcgttcacc tcaggagata tgggttccac ctcagccggc gcgataacac
     4441 gcaggtgggc ggggttgaca taatatttga accggtaaaa gtcttcctgc acctcgtacc
     4501 agtcatcccc tctggcgtct tgcagaatgc cggtgatcca atgcgtggaa gagtaataca
     4561 gccgataggc cacgatattt tttagcagcg ggttccagat ggcgtcggtg aagggcacgg
     4621 tcacctcccc cagcaaacct ttcacgggga tgtcttgccg ggtttcattc agactgatct
     4681 ttaccggctg caccgacccg gaatgcacgt atccctgccc gtcaagctga taccacacgc
     4741 ggttgtgact gggctcagag gagctgactt ccatgcgggt gatgggcagc accaggtctt
     4801 tccacagggt tttcaagggt ttaccttctg cagaaggggt ggggaacata tccaccttgt
     4861 tggtggtgat gcggccaagc tgggcagccg tgtcttgcag gctgcgagtt tgcgcgtagg
     4921 cgtaagtcgt tgaatggatg gccagcaagg aaaggatgga ggcgctgctg agcttgagga
     4981 actcgcgcct gctcatgttg cgcaggtcta atgcttctgc catgtcaata atgcacgctg
     5041 accaaagtac caacgtcggc gaaatcgtat agccaggcgg cgtcgctggt gcgcatgttc
     5101 acgcagccat ggctcatggg ggtgccgaag ttgttatgcc agtatgtacc gtgaatgccg
     5161 tagcctctat agaaatacat cgtgtaaggc acatccggca ggtagtaatc atctccccgc
     5221 atatccgtgt aacggtattt cacatagatc tggtaatcac cggtgacggt tgggtgcagc
     5281 caggtgcccg tagataccag gaaagaagct accatctcat caccatcaaa ggcgtaaagc
     5341 atttgactgc tcaaattgat gtcgatccag tgcccttcat tgtccgtgcg gatggcaacc
     5401 ggttcttgcg cagccggcgg gtaggttggc cagggggtat ctgtgggcaa gggagtgttt
     5461 gtggcggtgg gtgtgaaggt actggtaggc gtaaaggttg gcgtggcggt tggtgtggcg
     5521 gttggggtgg ctgttgctgt gggtgtaagg ctgggcttta ccagcgcgtc cgcggggatg
     5581 ggggcagcgg aactgcgcgc gagcgcaatc caattgggta caatggccca gatcacgaaa
     5641 gctgccagcg cgatcagcac ggcgaccagc aagaaaatca gccacttgcg ctgggttttt
     5701 cttctcagca cgggttttct tgtacctggc gtaacagcct gtttcgctgg ctgcggttga
     5761 ccgggtagct cgcccttttc acggggagca gccgctatgg gacgagggct tgcaggctga
     5821 gcgccatcag gtttcgcggc gcgaacgcgg atgggctggg tctcatctgc cgccggactt
     5881 tggggctgag gtttcgcggc gggagtttcc tcaagagaag ttcgagcgag acggttgagc
     5941 gcccagcgca tgcctttggt ggcccactcg ctggtggggt tgatcgcgag cgccttttgc
     6001 aggtattgga ggctgaccgc tggtggtgat gccgcagcca gcagcaacca gggctcttcg
     6061 cgctcaggcg cgatctgggc agcctggtgc gcccactgcc tgacctccgc gaagtcgcgg
     6121 tttttcaaag ccgcctgggc gttgagaatg gcctgcctgt aagcgggaaa atcctgttta
     6181 ttcatggcac gggagtgggc tggacgaggg tgacgttttc attgcgaatg aagcaaagca
     6241 gcccatttac aattccctgg gcgaccaggt ctgcgttttc agttaagatt tgccggtcga
     6301 ggttcaggaa gccggtctcg atgataaccg ccgtggtcgt ggagtggatc tcatcaaagg
     6361 cgtgataata ggtcatatct tctgaggccg cgttgggatg gtaggtgagc ccagttgtct
     6421 gcgcataccg gtcagccatg cacagggtaa ggcgggtggc tttatcagga tacacgctat
     6481 ggatggcggc ggctactttg aagcctgtgg cttcattgct gatgaaatca caggtatcag
     6541 cgtgaatcga aaccagcgcc agcgccttgt actgtgaaag ccggtcgtcg aactcctcca
     6601 gcaggtcgac tgtgtaaccc tcagccatga gtttttgttg caccaacgag gcgattttca
     6661 ggttaacctg ctcttcggtg agcccatccg ggcataaggc acctgaatcg tttttatagt
     6721 gaccagagac gataccaatt ggcagcgcag gcgttgccgg cgtgttcacc tgaacaggag
     6781 cagaaatgcg ggggtttgcc tgccacgcct ggaagatgcg gtcaaacatc tcgccagaaa
     6841 aaagattgtt gggtgtgaac agggtaaaga gcgaggctaa tagaaaagcg gtgacgacga
     6901 tcgactggat gctgctccat atacggaagg gttgatggct atcctgttct tcctcttccg
     6961 actctggcaa tagggattcc tcctcttccg gcagaggttc aaagggcggc tcaacatggt
     7021 caaacaatga atcgtcgctc atccaatcac caggataaat tatagcaagg tttgtgccag
     7081 atgaaggttt ttccgcgccg cgacgcttga cgtaggcccg cggcatgagt ataaaaggga
     7141 gaggagaatt agattatgtt cactcgaaat gatcttcggc aggtaatgag acgctggccc
     7201 agcggagtgg ccatccttac aacttcagat gggcttacga cgcatggatt gagcgttaat
     7261 tctttcactt ctgtctctgt tgaccccgag atggtaacag tcaccctggc gaataccacg
     7321 cgcgctaaac gcattgtgga taggaccggc ctgtttgcca tcaacttgct tcctgagaca
     7381 caggcagaac tctctgatgt tttcgccggc agaataccag aacatgctga tcgcttcgcg
     7441 ggtgttgaga ccacgcctgg gctcacccag atacccctat taaaagccgc ttctgcgcat
     7501 gtggagtgcc gggtcgtcca cacttacgaa atgccgcatt ctaccctgta tgtagcggaa
     7561 gtgctgcgcg cggagaaaaa cgcagacgcg ccgccgttgg tgtactttaa ccgggagtac
     7621 cataggatac agtaatgacc aatccactaa cagaagaaga aaaacactac ctgctccagc
     7681 tggcccggcg aaccattgaa caggcgttaa aacaggcgcc agaacctgaa atcgatttag
     7741 ggatgctctc tgcagcgctc aaggaacctg gggcgtcctt tgtaacgctt accatccggc
     7801 gtaacctgcg cggatgtatc ggcagcctgg aagcccgcca ggcgctggct tttgacgtgc
     7861 gcgaacacgc tcgccaggca gcctttgaag attatcgttt tcctcctctc tcgaaggctg
     7921 aattacccgc gaccagcatc gaaatttcaa ggctgactgc accgcttgac ctggaatacc
     7981 aacaaccatc cgacttaccg cgaatcctgc ggccaggcat tgacggggtg atccttcaag
     8041 atgggtacag gcgggcgact tttttgccgc aggtgtggga gcagctcccg aatgtggatg
     8101 attttctttc acacctctgc gtaaaaatgg gttccccggc tgacctgtgg aaaagaaaaa
     8161 cgctgcaggt aaaaacctac agcgttgaag aatttcatga ataaaggaag ttctttttct
     8221 ctcacttcga gagtgatcta gaagtggtgt gtttcgacaa cattcaatac gaaaatcctg
     8281 caacgttttt gtgatggatc agtggtttca ggaaagcagg ctcttatcaa agccagtgcg
     8341 ttttcaagct tctcgtcttc ggcaacgcaa agcagggtcg aaagtcctct tctgagaaaa
     8401 ccgcccgtcg aggcgatggt ggtgacgcgg tagtctccgt gggtcagggc ggacgtgacc
     8461 atttctgaat catcatcatt tacgatagca atgatcattt tcatgactat aactcctcgt
     8521 aatgttcgat ctcaagggcg aaaacggtgg cgccgcccac agtcacaggg gtaggcgcgg
     8581 gcaggggcag gggggcgctt tccagcggca cagcgatgta ttcgatacgt tggcggcagt
     8641 tatcatgcag tgcgtttagt atctgcggct gctgttcttg tggaatgcca attatcagcg
     8701 tggcgttgcg ccgacctaaa aaagcgccgg tgctcggcag acggatgagg gatatattca
     8761 tatcgttgag gatattttct accaggtcag cgtcctgcgc ctggactacc gccatcagca
     8821 tggtgttggg ggtggatgcg ggttgttcac tcatcgccca tctccttctt cctcgctcat
     8881 tccagggtta tcctgctggt acctggctga ttcaggtata aaacttctcc agattgcagg
     8941 tgaatttcag tcaggtgatg ctttaacagc agttccaact gattatattg taccgtaact
     9001 ggaggttgtt ttccatcata atgcctgatg accaactcgc ggttggataa tttcatcaga
     9061 ccgtgcagtc taagaaagag cgcagtaggc gcaagctcgg ttattcgtga ggttgaacag
     9121 cgagaacggc tgccctggtc agatggccct tttcccaggt ctttgagaaa atgattttga
     9181 aacaactggt cagcctggtc gaatttgccg tatctcacca gaccatcgat taccattgca
     9241 gccaggtagg atgggagcaa caaaccaggc gctggctctg ataccgggaa gacagccaaa
     9301 ccatggtctg ttagataaga tttgactttg gcgcgcgagg ttattctgcg ggagtctttt
     9361 tcactgagaa aaccagcata acaggccata ag
//
LOCUS       DAOSPM010000069         9307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_7351_length_9307_cov_16.490272, whole genome shotgun sequence.
ACCESSION   DAOSPM010000069 DAOSPM010000000
VERSION     DAOSPM010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7351_length_9307_cov_16.490272"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..944
                     /gene="gnd"
                     /locus_tag="PLW25_06825"
     CDS             33..944
                     /gene="gnd"
                     /locus_tag="PLW25_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HOS54236.1"
                     /translation="MQIGMIGLGKMGMNMAQRLIKGGHSVIGNATKETTIREAEALGV
                     KGARSLQELTANLAQPRVVWLMVPAGAVTEEVLGSLTPLLEMGDIVVDGGNSNYKDTT
                     RRGQVLMEKGLRFVDVGTSGGIWGLTEGYSLMIGGDPATVEYLRPIFETLAPAPDQGW
                     GRVGPSGAGHFTKMVHNGIEYGMMQAFAEGFEILKEKREFELDLHRVSQIWQRGSVIR
                     SWLLDLIENVMAEDSSLEDVEAYVADSGEGRWTVFEAIDLNVAAPVITASLQRRIRSR
                     QDGPYSDKLVAAMRHQFGGHEIKKKEG"
     assembly_gap    1061..1160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1161..1906)
                     /locus_tag="PLW25_06830"
     CDS             complement(<1161..1906)
                     /locus_tag="PLW25_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOS54237.1"
                     /translation="MNQTLRIIHHRRSTRKYAATPITSEEKDAILQAALRAPTAGNMM
                     LYTIIEVEDQALKDRLSVSCDNQPFIASASYVLLFLADYQRWVEAFNWSDAPRLAMEQ
                     GLQPRLPQEGDLLLACCDALIAAQTAVIAAESLGIGSCYIGDIMENYEVHREMFRLPR
                     FTFPVTLLCFGRPYAEREHPGLTRRFGRNIIVHRDHYTPVDADAFPAMLSRAGQQSPD
                     EVDVEAARKMAQGIYTRKFVSAFSVEMTRSV"
     gene            1972..3015
                     /locus_tag="PLW25_06835"
     CDS             1972..3015
                     /locus_tag="PLW25_06835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS54238.1"
                     /translation="MDRNKVSELTGLLTRALYHRQALLEQPPRSAIRLFNGFYEGFPP
                     LVVDLYARTLVVFTHKLDAADSLALAQMVQQHLLAQLDWIGCVLLKQRSSTHPDAKKG
                     VVLYGAQPDTVVWENGVQYALALRLNQDAGFYLDTRGLRAWLKENASNQSVLNTFAYT
                     GSLGVAALAGGASAVTQIDRSARFLEVARASLRLNALDETKMKTSAVDFFVAAGQMRR
                     KRETYDIVIIDPPFFSLTPRGRVDQVKETTRLINKARPLVTDGGYLVVVNNALFLSGR
                     EFIGELEALTENGLLTIEHIIPVGEDVTGYAETVVNQPPVDSAPFNHPTKIVVIKVKH
                     KARAADKANKEAK"
     gene            3012..3791
                     /gene="ygiD"
                     /locus_tag="PLW25_06840"
     CDS             3012..3791
                     /gene="ygiD"
                     /locus_tag="PLW25_06840"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HOS54239.1"
                     /translation="MIPVTRLPVLFVGHGSPMNAIEQNEFSLTWEELGRVMPRPRAVL
                     CISAHWETWDTRVTAMANPRTIHDFYGFPRELNEMQYPAPGSDELVELVKQKIKSTTV
                     VEDQEWGLDHGSWSVLCRMYPKAEIPVVQLSLNRQLSVQSHYLLGRELRPLCDDGILI
                     LGSGNMVHNLRMVTWEADAGYDWAVSYDADLKNWIEKGEHEQVVHYEEHGKPAELAVN
                     SAEHYLPMLYVLGAGYADEPVSFFNEKVIMGSISMRCFSLG"
     gene            complement(3778..4662)
                     /locus_tag="PLW25_06845"
     CDS             complement(3778..4662)
                     /locus_tag="PLW25_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS54240.1"
                     /translation="MTGDFSSKNVLVTGASSGIGYQAALDLAGKGAYIIGTGRDVTRC
                     EAARTRILQVHPEAQIEYLVADLASQTQIHDLARQAQLLLIEHGNRLDVLVNNAGLYS
                     SKRVLTEDGIELTFAVNHLAPFLLTHLLLPQLLNTPQGRVLGVSSQSHYHAFLSPLKA
                     YRPWLYLSLPVYATSNLCRVLFSAEFNRRTPTPHLHAWSVDPGLVNTEIGQKDKGFLS
                     NLVWRFRKTHGTSAEIPSQTILHLAFSAFHHIAADLYWKDSRPKEPSRLSRDAELAKR
                     LWEQSCRLCGIDDYFNPG"
     gene            4741..5937
                     /locus_tag="PLW25_06850"
     CDS             4741..5937
                     /locus_tag="PLW25_06850"
                     /inference="COORDINATES: protein motif:HMM:NF015185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS54241.1"
                     /translation="MSPQSKFTRLMMFALLYFTQGTILGYFASLNALYMLDNGLDMAA
                     VGIFSTIALIPFVIKILFGILSDRYNLLGLGHRKPYIFIGLLVQFACLIVVANINPGR
                     SYWLYVGLAFLLQLGMAFYDTCTDGLALDITPETEKGTLQGFMVGGRSVGVIVAASVA
                     GLIAQHLSWQGVFYFLAILTLVPIPFLFFIKEIQKTAEMRFQWSAFRAFKNVQVLAVA
                     AVGLVIFLVIVGANQLVNPSLDAKLGIDLSTAGLLTTVWGIGCVVGALAGGRVMDKLG
                     NQKALWLSILLVVPAMILLAMVANRPMMWAVIVFFGVSYGLSLAIYFALAMKYTQPAI
                     AASMFSILMAVTNVGQGIGLGLGGGLANAAGYPLTFLVFGALMLLVILFFPVLFGVKR
                     KGQGNQ"
     gene            5934..6344
                     /locus_tag="PLW25_06855"
     CDS             5934..6344
                     /locus_tag="PLW25_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54242.1"
                     /translation="MKKIYLTHCSKEKDPEFALNGEKVTPDRLYTSEGIQQFIKYCNE
                     HSHYWAILSDRYGVVFRDDHIKWYNQPPDTVTEEDYERLLQSFITRLSFFDEVYFYHR
                     PGETHPLFVRIIEAARAQGMKVSDFTQASMQKGE"
     gene            complement(6461..7069)
                     /locus_tag="PLW25_06860"
     CDS             complement(6461..7069)
                     /locus_tag="PLW25_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOS54243.1"
                     /translation="MQRTIVFDFGGVLIDWSPYHLYRKLLPSDDAVREFLEEIKFSEF
                     NKKLDAGYPFHKMKEELLEKYPHRYQLVRAFFERWMECTGEAVRPAVEIMREIKRAGY
                     PVYGLSNWSTETFPLLQHRYQFLPELDGYLLSGMAGVAKPDEEIFRIFLARFERNAED
                     CVFIDDTQANVDAARRLGFNAILFTSAGQLRVALVKIGVLKA"
     gene            complement(7238..8095)
                     /locus_tag="PLW25_06865"
     CDS             complement(7238..8095)
                     /locus_tag="PLW25_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54244.1"
                     /translation="MNSSLYPRNSFSEQEIKDLSAAMKVGILGTVNPQGLPHLTLITT
                     LMASSPQVVVWGQFMEGSSKKHVLTNPKTGFMVMGLDKNFWRGFADYSHSETSGKDYA
                     YYNNTPLFRYNAYFGVHRVHYMDLIAHTGKHPLPMQRIIPAAVMTMVAKTLSAKPQKT
                     PVLNPWTQAFFNKIDNLKFLGYVNAEGYPVIIPLIQAQALDREHLIYSLGAFGDELAQ
                     IPPYTPVAVFGMALSMEDVLVRGTYQGIKRVAGIRCGLVQLDWVYNSMPPTPRQIYPE
                     VVIEAVSEF"
     gene            complement(8136..9107)
                     /locus_tag="PLW25_06870"
     CDS             complement(8136..9107)
                     /locus_tag="PLW25_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOS54245.1"
                     /translation="MSSENVSTRGTILHLQRLSTEDGPGIRTTVFFKGCTLRCEWCHN
                     PESLSRHRQVQWLANRCIGCASCVAACEVHSLTLTVEGIVRDRGLCVTCGRCVEACPS
                     GAMEMLGKEVSAAELTAELLKDRAYFEKSGGGVTLSGGEPTLQPAFCAALMDALHAEG
                     VHVALDTCGLSAPGVFSELAAKADLILFDLKLMNPDEHKRWTGADNSRILRNLEIARD
                     FVSRSGGAKRLWIRTPLIPGATTAPDNLAGISAYLGASFDGSVERWELCAFNNLCRDK
                     YERLGMRWTFAETELLTAQQLTQMGEWARSGGFDPSRTFVTGAAKAS"
     gene            complement(9107..>9307)
                     /locus_tag="PLW25_06875"
     CDS             complement(9107..>9307)
                     /locus_tag="PLW25_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HOS54246.1"
                     /translation="YLRGYIENGGSALQINMLNADMLRDAQLHPEDYRHLLVRVTGYN
                     AYFTAIGKELQDEIIARESHQM"
BASE COUNT         2050 a         2392 c         2641 g         2124 t
ORIGIN      
        1 ttatgccaac atcgaggtca ttggaggaaa atatgcaaat cgggatgatc ggtctgggaa
       61 aaatggggat gaatatggcg caacgcctga taaagggcgg gcacagcgtg attggcaacg
      121 ccaccaaaga aaccacaatc cgtgaagccg aagcgctggg ggtgaaagga gcgcgctctt
      181 tgcaagagct taccgccaat ctggcacaac cacgcgtggt gtggctaatg gttcccgctg
      241 gcgcggtgac cgaagaagtg ctggggtcgc tcacccctct gttggagatg ggggatatcg
      301 tcgtggatgg cggaaattct aactacaaag acactacccg ccgcggtcag gttctgatgg
      361 aaaaaggttt gcgttttgtg gatgtgggaa ccagcggagg catatggggg ctcacagaag
      421 gctacagcct gatgataggt ggtgatcctg ccaccgtgga gtacctgcgc ccgatttttg
      481 aaacgctggc gccagcgcct gaccagggct ggggcagagt tggcccatct ggggcggggc
      541 attttaccaa gatggtacac aatggtattg aatacggtat gatgcaggcc tttgcagagg
      601 ggttcgaaat cttgaaggag aagcgggagt ttgaactcga cctgcaccgc gtttcgcaga
      661 tatggcagcg tggcagcgtg atacgctcat ggttgttgga cctgatcgaa aacgtgatgg
      721 cagaagactc gtcgcttgag gatgtcgaag cctatgtggc ggattcaggc gagggacgct
      781 ggaccgtgtt tgaagccatt gacctgaatg tggctgcacc ggtgatcacc gcttcgctgc
      841 aacgacgtat ccgttcgcgg caggatggcc cgtattccga taaactggtc gcggccatgc
      901 ggcatcagtt tggcggtcac gaaatcaaga aaaaggaagg ctagaggatc atcaacacgc
      961 gcttgtggct gcttaactca gcatagagcg cgtccacctg ctcgcggctc tgggcaataa
     1021 agtggaagga gaccgagcgg taatggttgt tgttggagag nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn acagagcgcg tcatttccac tgaaaaggca gaaacgaatt
     1201 tccgtgtata aattccttgc gccatcttcc tggcggcttc tacgtccact tcatctgggg
     1261 actgctgacc agcgcgcgac agcatggcgg ggaacgcgtc cgcgtctacc ggcgtgtaat
     1321 gatcacgatg aacgatgata ttgcgtccaa agcgcctggt taaccccggg tgctcacgct
     1381 ctgcgtaagg tctgccgaaa cacaacaggg taactggaaa ggtgaagcgc ggcaggcgga
     1441 acatttcgcg gtgcacctcg taattctcca taatgtcacc aatatagcac gaaccgattc
     1501 ccagcgattc agcggcgatg accgcggtct gggcggcgat gagcgcatcg cagcatgcca
     1561 gcagaaggtc gccctcctgc ggcagccggg gttgcagacc ctgttccatg gccagccgcg
     1621 gcgcatcgct ccaattgaaa gcctccaccc agcgctgata gtctgccaga aagagcagta
     1681 cgtaagaggc gctggcgata aacggctgat tgtcgcagct taccgacagc cggtctttga
     1741 gcgcctggtc ttcaacctca atgatcgtat acagcatcat attgcctgcg gtaggtgcgc
     1801 gcagcgcagc ctgcaggatg gcatcttttt cttcggaggt gatgggtgta gcggcgtact
     1861 tacgagttga acgtcggtga tgaatgatac gcagggtctg gttcatgggc gccttctgac
     1921 caggcaagga tatgcgattt ttaaatctac ctgaccgatt acaattatgc catggatcga
     1981 aataaagtaa gtgaactgac ggggttgctc acccgcgcgc tgtatcaccg gcaggcgcta
     2041 ctggagcagc cgccgcgttc agccattcgc ttattcaacg gtttttacga gggattccct
     2101 ccgctggtgg ttgacctgta tgcgcgcacg ctggtggtct ttacccataa actggatgcc
     2161 gctgattctc tcgcgctggc ccagatggtg cagcagcatc tccttgccca actggattgg
     2221 attgggtgcg tgttgctcaa gcagcggtct tccacgcatc ctgacgcgaa gaagggggtg
     2281 gtgctatacg gggctcaacc ggataccgtt gtgtgggaaa acggtgtgca atacgcgctt
     2341 gcgttgcgcc tgaaccagga tgccgggttc tacctggaca cgcgcggatt gagagcctgg
     2401 ctcaaggaaa acgcgagcaa tcaatctgtg ctcaatacgt tcgcctatac tggcagcttg
     2461 ggggtggctg cgctcgccgg tggggcatct gccgttaccc agatcgaccg cagcgcgcgt
     2521 ttcctggagg tggcacgcgc gtccctcagg ctgaacgcgt tggatgagac gaagatgaag
     2581 accagcgcgg ttgatttctt tgtggcggca ggtcagatgc gccgcaaacg cgaaacatat
     2641 gacattgtca tcatcgaccc gcctttcttt tccctcacgc cgcgcggtcg agtagatcag
     2701 gtgaaagaaa ccacgcgcct tatcaataaa gcgcgccctt tggtaaccga tggcggctac
     2761 ctggtggtgg tcaataatgc cctgttttta agcgggcggg aattcatcgg cgaactggaa
     2821 gccctgaccg aaaatggcct gctgaccatt gagcatatca tcccggtagg ggaggatgta
     2881 acaggttatg ccgaaacggt tgtaaaccag ccgccggttg attcagcgcc gttcaatcac
     2941 cctacaaaaa tagtcgtgat caaagtgaaa cataaagcgc gcgccgctga caaggcaaac
     3001 aaggaggcga aatgatacct gtcactcgat tacccgtttt atttgtgggt catggcagcc
     3061 caatgaacgc cattgaacaa aacgagttca gccttacctg ggaggaattg ggacgggtaa
     3121 tgcccagacc gcgagcggtg ctttgcattt ctgcccactg ggaaacctgg gacacccgtg
     3181 tgaccgctat ggcaaacccc cgcaccatac atgatttcta tggattcccg cgcgaattga
     3241 acgagatgca gtatcccgcg ccgggatcag acgaactggt cgagttggta aagcaaaaaa
     3301 tcaaatcaac cactgtcgtt gaagaccagg agtggggatt ggatcatggc agttggtcgg
     3361 tgctctgccg catgtaccca aaggcggaga tcccggtggt acagctgagc cttaaccgtc
     3421 aactctccgt ccaatctcat tatttgctcg gcagagaatt acgccccttg tgcgatgacg
     3481 gcatactgat ccttggcagc ggaaatatgg tgcataacct gcgcatggtc acatgggaag
     3541 cagacgctgg ttatgactgg gccgtttcct atgacgccga cctcaaaaac tggatcgaaa
     3601 aaggcgaaca cgaacaggtg gttcattatg aggaacacgg gaaacccgct gaactggcgg
     3661 tgaattcagc cgagcattac ctgccgatgc tttacgtact cggtgcgggg tacgcggacg
     3721 agccggtcag ttttttcaat gaaaaggtca tcatgggctc gatctcgatg cgttgtttca
     3781 gcctgggttg aagtagtcat caatcccgca taagcggcag gattgttccc acaaacgttt
     3841 tgccagctcg gcgtcgcgag aaagccttga gggttctttt gggcggctgt ctttccagta
     3901 caggtcagcg gcgatatggt gaaaggcaga aaaggccagg tgcagaatgg tttgggaggg
     3961 aatttcagcg ctggtaccgt gtgtttttcg aaaacgccaa accaggttgg agagaaaacc
     4021 cttatctttt tgaccgattt ccgtgttgac caatcccggg tcaaccgacc aggcgtgcag
     4081 gtgaggtgtt ggtgtacggc ggttgaattc cgcgctgaag agcacccggc acaggttgga
     4141 tgtggcgtac accggcaggc tgaggtacag ccagggacgg taagccttca ggggagagag
     4201 aaatgcgtgg taatgagact gagaactgac ccccaaaacg cgaccctgcg gcgtgtttaa
     4261 caattgcggc agcagcaaat gagtgagcag aaaaggcgcc agatggttca ccgcgaatgt
     4321 cagttcgatg ccatcttccg taaggacgcg cttgctggaa tagagaccgg cattgttgac
     4381 cagcacatcc agcctgttcc catgttctat caggagaagc tgcgcctgac gcgccagatc
     4441 atgaatttgg gtttgagaag cgagatcggc gaccaggtac tcgatttggg cttctggatg
     4501 gacttgtaat atacgagtgc gcgccgcttc gcaacgggtc acgtcccgac cggtgccaat
     4561 tatataagcc cctttgcccg ccagatcgag cgccgcctgg tagccaatcc ctgaactggc
     4621 tccggtgacc aatacattct tgctgctaaa atctccggtc attttccctc cggtttgatc
     4681 gttttctttc tgtttgatta tttgtataat tttgacataa aaatttacaa aggaaaccct
     4741 atgagcccac aaagcaagtt cactcgcctg atgatgttcg cgctgctcta ttttacccag
     4801 ggaacgatat tgggatattt cgcttctctc aacgcgctgt acatgctgga taatggatta
     4861 gatatggcgg ctgtgggcat cttttccacc attgccctca tcccttttgt catcaagatc
     4921 ctttttggca ttctgagtga ccgttacaac ctgttagggt tgggacaccg caaaccgtac
     4981 atcttcatcg gtctgctggt gcagtttgcc tgcctcatcg ttgtggccaa catcaacccc
     5041 ggtagaagtt actggcttta tgtggggctg gctttcttgc tccaactggg tatggcgttt
     5101 tatgatacct gcaccgatgg gctggcgctg gatatcacac cagaaacaga aaaaggcacc
     5161 ctgcagggtt ttatggtcgg cgggcgttca gtgggcgtga ttgtggctgc ttctgttgcc
     5221 gggcttatcg cgcagcacct ctcatggcaa ggcgtgtttt actttctggc catcctcact
     5281 ttggtgccaa tcccgttctt gttctttatc aaggaaattc agaaaacggc tgaaatgcgc
     5341 tttcaatggt cagcttttcg cgcgtttaaa aatgtgcagg tgctggcagt agccgcggtg
     5401 gggctggtca ttttcctggt gattgtgggg gcgaatcaat tggttaaccc ctctcttgac
     5461 gccaaactgg gcattgacct cagcacagcc ggtttgctca ctactgtgtg ggggataggc
     5521 tgtgtggttg gcgcgctggc tggcgggagg gtgatggata agctgggaaa ccaaaaagcg
     5581 ctgtggctgt ccatcctgct cgtggtaccg gctatgatat tgctggcgat ggtggccaac
     5641 cgaccgatga tgtgggcggt gatcgtcttc tttggcgtct cctacgggct ctctctggcg
     5701 atttactttg cgctggcgat gaagtatacc cagcctgcca ttgccgcatc gatgttctcc
     5761 atcctgatgg cggtcaccaa tgtgggtcag ggtatcggcc tggggctggg cggagggctg
     5821 gctaacgcgg ctggttaccc gctgactttt ctagttttcg gcgcgttgat gctgctggtc
     5881 atcctcttct tcccagtgtt gttcggcgtg aaaagaaagg gacaaggcaa ccagtgaaaa
     5941 aaatctacct cactcattgt tccaaagaaa aagaccctga atttgcgctg aacggagaaa
     6001 aggtcacgcc tgaccggtta tacacctcag aggggattca acagttcatc aagtattgta
     6061 atgagcattc tcactactgg gcaattctct ccgaccgcta cggagtggtt ttccgcgatg
     6121 accacatcaa atggtacaac caacctcctg acacggttac cgaagaagat tacgaaaggc
     6181 tgcttcaaag tttcatcaca cgcctgtctt tttttgacga ggtttatttt tatcaccgcc
     6241 ccggggaaac ccatccgctc tttgtgcgaa ttattgaagc ggccagggcg caagggatga
     6301 aagtcagcga tttcacgcaa gcctcgatgc agaagggcga gtaacacctg gagggccaga
     6361 atgagcctgg cagagaaagc ccgtccgcgc ccgcggtata cgccctacaa gttgtgaaag
     6421 cgaggtttct ttcaggcgct ccccaacaga gaaaatggtt tcaagccttc aatacgccaa
     6481 ttttcaccag cgcaacgcgt agttggccgg ctgaagtaaa gagaatggcg ttgaacccga
     6541 gccgcctggc ggcgtcgaca ttcgcctgcg tgtcatcgat aaacacgcaa tcctcggcgt
     6601 tgcgctcaaa acgcgccagg aagatcctga agatttcctc gtccggtttg gctacacctg
     6661 ccatccctga aagcaggtaa ccatccagtt caggcaggaa ctggtagcgg tgctgcagga
     6721 gcgggaaggt ttcggtggac cagttgctca gcccgtacac cgggtaaccc gcgcgtttaa
     6781 tttcgcgcat gatttccacg gctggtctga cggcttcgcc agtgcattcc atccagcgtt
     6841 caaaaaaagc gcgcaccagt tgataccggt gcgggtattt ttcaagcaat tcctctttca
     6901 ttttgtggaa aggatagcca gcgtccagct ttttgttgaa ttcactgaac tttatttctt
     6961 ccaaaaactc gcgcacagcg tcgtcgctgg gcagcaactt gcggtaaaga tggtagggac
     7021 tccagtcaat gagcaccccg ccaaagtcaa acacgatggt tctctgcata gatggataat
     7081 tctaaccgaa gttcaacgac ccatgaccta ttaagcaatg tcagtaaaag ctgacccatg
     7141 gtcagaaatg ggtcgaaaca cctcattgat tgaatttcat cgctcgctcc aggcggtctg
     7201 aagcctggag catcatttga aaatgcttcc ttgaccttta aaattcgctc actgcttcga
     7261 tgaccacttc agggtagatt tgccgggggg tgggcggcat ggaattgtag acccagtcta
     7321 actggacgag accgcatctt atgccggcaa cgcgcttgat tccctggtaa gtgccgcgca
     7381 ccagcacatc ttccatgctg agcgccatgc cgaacactgc cactggagtg tacggtggaa
     7441 tttgcgccag ctcatcacca aacgcgccca atgaatagat gagatgctcg cggtcgagcg
     7501 cctgcgcctg gataagcggg atgataaccg ggtagccctc cgcgttgacg taaccgagga
     7561 acttaaggtt atcaatcttg ttgaaaaatg cctgtgtcca gggattcagc acaggggtct
     7621 tttgtggttt cgcgctgagc gttttagcca ccatggtcat caccgcggcg gggataatgc
     7681 gctgcatcgg cagcgggtgc ttccccgtgt gagcgataag gtccatataa tgcacgcggt
     7741 gcacaccaaa atacgcgttg taacggaaaa gcggggtgtt gttgtagtac gcgtaatcct
     7801 ttcctgaagt ctctgagtga gaataatcag cgaaaccgcg ccagaaattc ttatccagcc
     7861 ccatcaccat gaatccggtc ttcgggttgg tgagcacatg tttcttgctg ctgccttcca
     7921 tgaactgacc ccataccacc acctgtgggc tgctcgccat gagcgtggtg atgagggtga
     7981 gatgcggcag cccctgcggg ttcaccgtgc ccagaatacc gaccttcatt gccgcggaca
     8041 ggtctttgat ctcttgttct gaaaaactgt tgcgggggta aagagaggag ttcatggttt
     8101 tagggtctcc attcaagcat tatttctcga aataatcaac tggctttggc tgccccagtg
     8161 acaaaggtgc gcgaagggtc gaagccgcct gacctggccc attcacccat ctgtgtcagt
     8221 tgttgcgcgg tcagcagttc tgtttcggca aaggtccagc gcatgcccag gcgttcgtat
     8281 ttgtcgcggc acaggttgtt gaaggcgcat agctcccagc gctctacact gccatcgaac
     8341 gatgcgccga ggtacgcact gatacctgcc aggttgtcag gggctgttgt tgcgccgggg
     8401 atgagcggcg tgcgaatcca caggcgtttt gccccgcctg aacgagacac gaaatcgcgc
     8461 gcaatttcca ggttgcgcag gatacggctg ttgtccgcgc ctgtccaacg tttgtgttca
     8521 tcggggttca tcagtttcag gtcaaacaaa atgaggtcag cctttgccgc cagctcactg
     8581 aacacaccag gcgcgcttaa cccgcaggta tccaacgcca catgcacgcc ctcagcgtgc
     8641 aaggcgtcca tcagcgcagc gcaaaacgcc ggctgcaggg ttggttcccc tcctgagagg
     8701 gtaacaccgc cgccggattt ttcgaagtaa gcgcggtcct tcagcagttc ggcggtcagt
     8761 tccgctgcgc tcacttcttt tcccagcatt tccatcgcgc ctgatggaca ggcttcaaca
     8821 cagcgcccgc acgtcacgca caacccgcgg tcgcgcacaa tgccctcaac ggtgagcgtg
     8881 agactatgca cctcgcaggc agccacacag ctcgcgcagc cgatgcagcg gttggcgagc
     8941 cactgtacct gcctgtgccg ggaaaggctt tcagggttgt ggcaccattc gcagcgcagc
     9001 gtgcagccct tgaagaaaac cgtggtgcga atccccgggc catcctccgt ggagaggcgt
     9061 tgcaggtgca ggatagtgcc gcgggtagaa acgttttcgg atgacatcac atctggtggc
     9121 tttctcgggc gatgatctca tcctgcagtt ctttgccaat tgcggtgaag taagcgttgt
     9181 aaccggtcac gcgcaccaac aggtggcggt aatcttcggg gtgtagctgc gcgtcgcgca
     9241 gcatgtctgc gttgagcatg ttgatctgca gcgcgctgcc gccattttca atataaccgc
     9301 gcaggta
//
LOCUS       DAOSPM010000070         8577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_8191_length_8577_cov_16.888289, whole genome shotgun sequence.
ACCESSION   DAOSPM010000070 DAOSPM010000000
VERSION     DAOSPM010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8191_length_8577_cov_16.888289"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..825
                     /locus_tag="PLW25_06880"
     CDS             73..825
                     /locus_tag="PLW25_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOS54247.1"
                     /translation="MTEIQSLARGLRILLMLSDSLDGSTVTEVAENLGVDKGSASRLV
                     NTLVKYGFAEKDPVSRRISLGPQIVSLSRSLLTRMPLREVAKPYLRELMRSTGECAHI
                     GILSQGKVLYIDQVESPASLRVNAEVGFLAPLHCTALGKVLLAFGRLPLPESLERYTD
                     STLVTQFALKKNLASVREQGYAIDDEEFDIGVRCIAAPIFDFRNKVVGSIGISGPTTR
                     MTPGVLQTLVQQVLEISDKLNGRMKFNQGREQ"
     gene            825..2711
                     /locus_tag="PLW25_06885"
     CDS             825..2711
                     /locus_tag="PLW25_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOS54248.1"
                     /translation="MKVYSVEELLATRVKRANLISSAGGLRQALASASLDRKTDLTLS
                     EALVIGLIEQGVRTYYSVLGHGSTEVGEVLRVYQNAGLVRVFGLRSEIEASHAAAALR
                     WVKGEKAAVVTSIGPGAMQALAASLVPASNGIGVWYIFGDETTEDEGYNMQQIPRHQQ
                     AGFLKLTSVMGAAYSLHTPLALQSALLAGNVVVNHPYRPGPFFLLLPMNTQAALMENF
                     NLDELGSPAPVRLNAAVGAYDEAANWIKTSKRVLVKVGGGGARAGDELVKFLELSGGV
                     LVHTPIATGCVPYSHPANMSVGGSKGSICGNFAMENADLLVAVGTRAVCQSDSSRTGY
                     PNVKKVININADLEDALHYNHTLPLVGDVRLTLTKLNQMLEQENTPIDKAGWLKVCAE
                     KKKRWEEIKAARLAAPTLFDDYWQQEVLTQPAVLQTAIAWAKARGAVRFFDAGDVQAN
                     GFQLTEDEVPGQTFTDTGASYMGFAVSALLASGTASHPFYAVAFTGDGSFTMSPQILV
                     DGVEHGAQGCILLLDNGRMGAISGLQLDQYHHEVATANHILVDYLQWAKGVPGVLALD
                     GGNSLDELQAALNQAGNHPGLSLIRVKVYFGASELGGMGVYGRWNVGNWCAKTQQLRH
                     EIGL"
     gene            2720..3568
                     /locus_tag="PLW25_06890"
     CDS             2720..3568
                     /locus_tag="PLW25_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOS54249.1"
                     /translation="MTELSYEQITSLEKIARSLRADSLRFIYGRGSGHPGGALSAAEI
                     LAVLYFHELRIDPQHPDWSERDRFILSKGHASAVLYAALARRGFFDKNELKNWGEVDC
                     PHQGHPDRLKTPGVDMTSGILGHGVAVGVGLALAARMKKSPSRTYVLIGDGESQAGIV
                     WEGAAAAAKYRLSNLVVILDLNDVQLDGPMHEIMPMDHMAQRWEACGWYVMQVNGHSV
                     RQLVEGLDLAREIHDKPVCIIARTTKGKGVSFMENDSFWHGNVPSKEQLEQALLELGV
                     EEEKNG"
     gene            3561..>3970
                     /locus_tag="PLW25_06895"
     CDS             3561..>3970
                     /locus_tag="PLW25_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOS54250.1"
                     /translation="MAELSMREGYGRALADYGKMNPKVVAVDVDTSASTLSHFFAREH
                     PERFFNVGIAEPCAVDVGVGLALGGYIPFVNAFAALLALRSLEQIRTCVCYANTNVKL
                     AASYAGLSDFKDGPTHFAMTDIANMRALPNMNIIV"
     assembly_gap    3971..4070
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4071..4367
                     /locus_tag="PLW25_06900"
     CDS             <4071..4367
                     /locus_tag="PLW25_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOS54251.1"
                     /translation="IHTIKPLDGEILLDAAKETGAIVTAEEHVRAGGLGGAVSELLAG
                     TYPIPIERVGIRDEFCPTGRNIDGLLDACGLSVAEIMLAARKVKKMKSGGAEKQ"
     gene            4364..5002
                     /locus_tag="PLW25_06905"
     CDS             4364..5002
                     /locus_tag="PLW25_06905"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOS54252.1"
                     /translation="MKIAVINEISAAAKNAEIIHALTGRGHELLNLGNTGAADEPQLQ
                     YIHTGLMSALLLHLKVVDFVVGGCGTGIGYLNSVLQYPGVVCGLIHSPLDAWLFARIN
                     DGNCASLPLNQGYGWAGEINLRMIFDELFTPKRGSGFPEHRAEPQRNSRFTLAEISQT
                     THQPFATIIRDLPLSVTGPALQYPEFSQVLRESQQRDPMLEEAIHQILESGG"
     gene            5004..6374
                     /locus_tag="PLW25_06910"
     CDS             5004..6374
                     /locus_tag="PLW25_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS54253.1"
                     /translation="MSVSAAPQKLKAKTKWLYGIGDIGNALVNSSLQFFLMKFYTDAA
                     LILPVLAGNALLVGKIWDAINDPLFGWLTDRTNSKIGKRRVFMLFGAIPLALSVALLW
                     FVPPGLSQVMTFVWIAGTFIFFDSVWTLTNVPYYALTSELTDDYDERSSLTVYRMVLA
                     TPAYLIGAALTPMIVGFFAVQRTGFAVIGIVYGVIAALALLISAGGLRERPNVIRSEG
                     EVNPIKSLKEAFRNRPFIYLFVVYFIVNISFAFAKTLMAYFIEYQLMMKDAISIALGL
                     MLICVTISLPIWKWVSTKMDKGPAYAIGMGMGAVALLVLWFLPAKPTVLIYPIMVLAG
                     FGFGANWIFPWAMIADVADYDRVKSGKFRSGVYYGVWGLATKISEALALAAVGWVLTG
                     FGYVPNIAQTPHALLGIRLFFSIVPAVGIFVGLPLLFKYPLTRKSHAEIKAKLLAMDA
                     AAKNDA"
     gene            6392..7471
                     /locus_tag="PLW25_06915"
     CDS             6392..7471
                     /locus_tag="PLW25_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS54254.1"
                     /translation="MKPIRVGIVGCGRISDLHQLGYRGRDDAVIAAVCDNNAAHAKKK
                     AKAWGVQKVYTDYQHLLEDKEIDAVELLTPHHLHCPMTVQAARAGKHISVQKPMALSA
                     AEADQMIDAANKAGVILRVYETFVFYAPVVRAKEMIDNGEIGEVRAVRMHVSTGTGDT
                     GWKVPTSALLWRIDEKKCGGGQLVFDHGYHLFSVGYYLGGPVEKVFAWIDKTPVTETG
                     GIVKVDAPAMIQFQYKAPRCYGNLDVEHTPKMRIYSDYYADDDRIEVIGEKGILFINR
                     YTARTVDLPELMIFKDGKTNPIPVEGVEWHDSFIATTHNFIDNLKTGGQPQLDGPTGK
                     AVLQFTLAALQSAATGKVVRPDDVL"
     gene            7487..8155
                     /locus_tag="PLW25_06920"
     CDS             7487..8155
                     /locus_tag="PLW25_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOS54255.1"
                     /translation="MTYSLIPVPIHLVLIVLYMIARNRNDLKKTAIIQPSITFLAIVI
                     AGLSYLSPYVNPAYTTWILVGLVLCFIGDIFNIDMTNDKILFAAMVIFFIAYVEYAVA
                     FTRFGGFHTQDWLIGALMLVVYILLIRLYWKGLGNFRIPVMIFVLVISFMVTRAISTL
                     FSSQFSALSAWLVAFGGPLLFMGDVEYGLHRFHKPLKFHLGPLYYAGGQLLVALSCAY
                     LLFR"
BASE COUNT         2016 a         2000 c         2386 g         2075 t
ORIGIN      
        1 tagttgtgta atacgcaatt aagttgtatt atatgcaaat atcctataca ctctaattgt
       61 tcaaggtgaa aaatgactga gatccaatcg cttgcccgcg gattgcggat tctgttgatg
      121 ctttctgatt cgctcgatgg gagcactgtg acagaagtgg cggagaacct tggggttgac
      181 aagggaagcg cttcgcgcct ggtaaacaca ctggtcaaat acggcttcgc ggaaaaagac
      241 ccggtttccc ggcggatttc cctggggccg cagattgttt ctttgagccg tagcttactt
      301 accaggatgc cgttgcgcga ggtggcaaaa ccttacctgc gtgagctgat gcgctccacc
      361 ggtgaatgcg cccatattgg tatcctttcc cagggcaaag ttttatatat tgaccaggtg
      421 gaatcgccgg cttctttacg ggtaaacgcg gaggtagggt ttcttgcccc gctgcattgc
      481 accgcgctcg gcaaggtact gcttgctttt gggcgcctac ccctccctga atcccttgaa
      541 cgttacaccg actcaacact ggtgactcaa tttgcgctga agaaaaacct ggcgagtgtg
      601 agggaacaag gctacgcgat cgatgatgaa gaatttgata ttggtgtgcg ttgcattgct
      661 gcgcccatct tcgatttcag gaataaggtg gttggcagta tcggcatatc aggaccaacc
      721 acccgcatga ctccaggcgt attgcaaacc ctcgtgcaac aggtgcttga gattagcgac
      781 aaactcaacg gcaggatgaa attcaatcag gggagagaac agtaatgaag gtttattcag
      841 tagaagaact gttagccacc agggtcaagc gcgcgaatct tatttcatcg gcaggagggc
      901 tgcggcaggc gttggcctct gcgagccttg atcggaaaac cgacctgacc ctctcagaag
      961 ccctggttat tggattgatc gagcaaggag tacgcacgta ttattcagtt ttaggtcacg
     1021 gttcgactga ggtaggtgaa gttctgcggg tataccagaa cgcaggattg gtgcgtgttt
     1081 ttgggctgcg cagtgagatc gaagcatcac acgcggcggc agcgctgcgt tgggtaaaag
     1141 gggagaaagc cgcggtggtt acctctatcg ggccaggagc gatgcaggca ctagctgctt
     1201 cgctggtgcc ggcatcgaat ggcatcgggg tttggtatat cttcggcgat gaaacaacgg
     1261 aggatgaagg gtacaacatg cagcagatcc cccgtcacca gcaggcaggg tttttaaaac
     1321 taacctctgt gatgggcgct gcctacagcc tgcacacacc gctggccttg cagtctgctt
     1381 tgctggctgg caatgtggtc gttaatcacc cctatcgacc tggtccattc tttttattgt
     1441 tgcccatgaa cactcaggca gcgctcatgg aaaacttcaa tctggatgaa cttggttcac
     1501 ctgctccggt acgattgaac gcggctgtag gagcgtatga cgaagcggcg aattggatca
     1561 aaacttcaaa acgagtgttg gtcaaggtgg ggggcggggg agccagggct ggagatgagt
     1621 tggtgaagtt tcttgaatta tcaggcgggg tgctggtgca cacacccata gccaccggtt
     1681 gcgttcccta ctcgcacccg gcgaatatga gcgtgggcgg atcgaaaggc tcgatttgcg
     1741 gcaatttcgc catggagaat gctgatctgc tggtcgctgt gggcacgcgg gcggtctgcc
     1801 agtcagattc ttcaaggacc ggttacccga acgtaaaaaa ggtgatcaat attaacgcag
     1861 acctggagga tgccctgcat tacaaccaca ccctgccttt ggtgggagat gtgcgcctga
     1921 cattgacgaa gctaaaccaa atgctcgaac aggaaaatac tccaatcgat aaggcgggat
     1981 ggctgaaagt ttgcgcggaa aagaaaaagc gctgggagga gataaaagca gccaggttgg
     2041 cggctccaac gcttttcgat gactactggc agcaggaggt ccttactcag ccagccgtcc
     2101 tgcagaccgc gattgcttgg gcaaaagcgc gcggcgcggt gcggttcttt gacgcggggg
     2161 atgtgcaggc gaacgggttt caattgaccg aagacgaagt tcctgggcaa acatttacag
     2221 atacaggagc cagttacatg ggcttcgcgg tttctgccct gctggcgtcc ggaacggcat
     2281 cccacccatt ttacgccgtg gcgtttacgg gagacggctc gtttaccatg agcccgcaaa
     2341 ttttggttga tggtgttgag cacggcgcgc aaggctgcat ccttttactg gataatggaa
     2401 ggatgggggc gatttcaggg ttacagcttg accagtatca ccatgaagta gccaccgcta
     2461 atcacatcct ggtagattac ctgcagtggg ccaagggggt tccaggcgtg ctcgctcttg
     2521 atggtggaaa ttcactggat gaattacagg ccgcgttgaa ccaggccgga aatcaccctg
     2581 ggttatccct tattcgggta aaagtttact ttggagcaag tgaactgggt ggtatgggtg
     2641 tgtatggtcg ctggaacgta gggaactggt gcgcgaaaac acaacaatta cgccatgaaa
     2701 ttggccttta ggagaggtta tgacagaact atcctatgaa cagattacat cactggaaaa
     2761 aatagcgcgg tctttgcgcg ctgacagcct gcggttcatc tacgggcgcg gttcgggcca
     2821 tcctggcggg gcgttatcgg cagcggagat actggctgtc ttatatttcc atgaattacg
     2881 aatcgatccc caacacccag attggtcgga aagagacagg ttcatactga gcaaagggca
     2941 cgcttcagcg gtgctctacg cagcgctggc caggcgcggt ttctttgata aaaacgaact
     3001 gaaaaactgg ggagaagtgg attgcccgca ccaggggcac ccggaccgtt taaaaacccc
     3061 cggagtagat atgaccagcg gcatcctcgg gcatggagtg gcggttggcg ttgggctggc
     3121 gttggcggcg cgcatgaaga aatctccttc gcgaacatat gtgctcatcg gagacggtga
     3181 atctcaggcg ggaatcgtat gggaaggagc ggccgcagcg gccaagtacc gcctgtccaa
     3241 cctggtggtt atcctggacc tcaatgacgt gcagctcgat ggtccaatgc atgaaattat
     3301 gcccatggac catatggcgc aaaggtggga agcctgcggc tggtatgtga tgcaggtaaa
     3361 tggtcacagc gtgcgccaac tggtggaagg tttggatctt gctcgagaga tacatgacaa
     3421 acctgtatgc atcatcgccc gtacgaccaa aggaaaaggg gtgtccttta tggaaaacga
     3481 ttccttctgg catggcaacg taccgagcaa agaacaactt gagcaagcgc tgcttgaact
     3541 tggcgttgag gaggagaaaa atggctgagc tctccatgcg tgaaggatac ggacgcgcat
     3601 tggcggatta tggaaagatg aaccccaagg tcgtggcggt ggatgtagat acttccgctt
     3661 ccacgctctc tcactttttt gcccgtgaac acccggaacg tttttttaat gtgggaattg
     3721 ctgaaccttg cgcggtggat gtgggggtag gtctggcgct tggcgggtat atcccctttg
     3781 tgaacgcgtt cgctgctttg ctggcattgc gctcacttga acagatccgc acctgcgttt
     3841 gctacgccaa taccaatgta aaactggcag ccagctatgc cggtttatct gatttcaaag
     3901 atggcccgac tcactttgcc atgacagaca tcgccaacat gcgggcactg ccgaatatga
     3961 acatcatcgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn attcacacca
     4081 tcaaaccgct ggatggcgag atcctgctcg atgcggctaa ggagaccggc gcgatcgtaa
     4141 ccgctgaaga gcatgtacgt gccggtggat taggcggcgc tgtatccgag cttcttgcgg
     4201 gaacttaccc aatccccatt gagcgggtgg gaattcgcga cgagttttgc ccgacgggga
     4261 gaaatatcga tggattgctg gatgcctgcg ggctatccgt ggcggagatt atgttagcag
     4321 cgcggaaggt gaagaaaatg aaatcaggag gggcggaaaa gcaatgaaaa ttgcagttat
     4381 taatgaaatt agcgcggcag caaagaatgc ggaaatcata cacgcgctca cagggcgggg
     4441 tcacgagtta ttgaacctgg ggaatacagg cgcagcggac gagccgcagc ttcaatatat
     4501 tcacaccggt ctgatgtctg cgcttttatt gcacctgaag gtggtggatt tcgttgtggg
     4561 cggatgtggt acggggattg gttatctcaa ctcggtattg caatacccgg gagtcgtttg
     4621 cggccttatc cattctccgt tggacgcctg gttgtttgcc cgtataaatg atggtaattg
     4681 cgcttctctc ccgttaaacc agggttatgg ttgggcggga gagataaacc tgcgcatgat
     4741 ttttgatgaa ttgttcaccc caaaaagggg gagcgggttc cctgaacacc gtgctgaacc
     4801 ccagcgcaac tcccgcttca cattggcgga aatttcacag acaacccatc agccatttgc
     4861 aacgatcatc cgtgacttac ctttgtcggt gaccgggccg gcactacaat accctgaatt
     4921 ttctcaggtt ttacgggagt cccagcaaag ggaccctatg ttggaagaag caatccatca
     4981 aatacttgaa tcaggaggat aaaatgtcag tctcagcagc gccacagaaa ttaaaagcga
     5041 agaccaagtg gttgtacgga attggggata tcgggaacgc gctggttaac agctcgctcc
     5101 agtttttcct gatgaaattc tacacggatg cagcgctcat tttgcctgtg ctggcaggga
     5161 atgcgctgct ggtaggcaag atctgggacg ccatcaacga tccgcttttt ggctggctta
     5221 ccgacaggac aaattccaag atcggaaaac ggcgcgtatt tatgctcttc ggcgcaattc
     5281 cgctggcgtt gtcggtggct ttgctctggt ttgttccgcc cggtctcagc caggtgatga
     5341 ccttcgtttg gatcgctggg acattcatct tctttgattc agtctggacg ctgacgaacg
     5401 taccatatta cgcgttgacc tcggagctga cggatgatta cgacgaacgc agcagcctga
     5461 cggtttaccg catggtactg gctaccccag catatttgat tggcgccgcg ctcactccca
     5521 tgatcgtcgg tttttttgct gtgcagcgca ccgggttcgc ggtaatcggc attgtttatg
     5581 gtgtgatcgc tgccctggca ctgctcattt ccgcgggagg gttgcgagaa cgccccaatg
     5641 tcattcgctc tgaaggagaa gtcaacccga tcaaatccct caaagaagcg tttcgaaatc
     5701 gccccttcat ttacctgttt gtggtctatt tcattgtgaa catctctttt gcctttgcaa
     5761 aaacgcttat ggcttacttt atcgagtacc agttgatgat gaaagacgcc atatcgattg
     5821 ctttgggctt gatgctcatc tgtgtaacca tctcccttcc catatggaaa tgggtgtcta
     5881 caaaaatgga caaaggaccc gcgtacgcta ttggcatggg aatgggcgcg gtggcgctgc
     5941 tggtgctgtg gttccttcca gcaaaaccca cggtgttaat ttacccgatc atggttctgg
     6001 cggggttcgg ctttggcgcg aactggatct tcccctgggc catgattgcg gatgtcgcgg
     6061 attacgaccg ggtaaaatcg ggcaagttta gaagcggcgt gtattacggc gtttgggggc
     6121 tggcaacgaa aatatcagaa gcgcttgctc ttgccgctgt tggttgggta ttgaccggct
     6181 ttgggtacgt acccaatatt gcccaaaccc cgcacgcgct gctgggaatc cgcctgttct
     6241 ttagcattgt gccggcggta ggaatctttg tggggttacc actactgttc aaatatcccc
     6301 ttacgcgtaa aagccatgct gaaattaaag caaagctgtt agccatggac gccgcggcga
     6361 aaaacgacgc ataaccaaga aggatgatga catgaaaccg atcagggttg gcattgttgg
     6421 ctgtggcagg atctctgacc tgcaccaatt gggttaccgc ggacgggacg acgcggttat
     6481 cgccgcggta tgcgacaata acgctgcgca cgccaaaaag aaggccaaag cctggggtgt
     6541 tcagaaagtt tacacggatt accaacatct gctggaagac aaggagatag atgcggtgga
     6601 actgctcaca ccgcatcacc tccactgccc aatgaccgtg caggcagcca gagcgggtaa
     6661 gcatatatcc gtgcagaaac ctatggcact ctcggcagcg gaagctgacc agatgatcga
     6721 cgcggcaaat aaagccgggg tgattttacg ggtatatgaa actttcgtat tttacgcccc
     6781 ggtagtccgc gcgaaagaaa tgattgacaa cggagagatt ggcgaggtga gagcggttcg
     6841 catgcacgtc agcaccggca caggcgatac aggctggaaa gtgccgactt ccgccttgtt
     6901 atggcgcatt gacgaaaaga agtgtggcgg gggtcaactg gtgtttgacc atggctacca
     6961 tctgttttcg gtgggctatt acctgggagg accggtggaa aaagtatttg cctggatcga
     7021 taaaacgccg gttacggaga cgggcgggat tgtcaaggtg gatgcgcctg ctatgatcca
     7081 gttccaatac aaggctccac gttgttatgg caacctggat gtggagcaca caccaaaaat
     7141 gcgcatttat tcagattatt acgctgatga tgaccgtatc gaggtgattg gagaaaaagg
     7201 aattttgttt attaaccgct acaccgcgcg cactgtggac ctgccggaac tgatgatctt
     7261 caaggatggg aagaccaatc ccatccctgt ggaaggggtg gaatggcatg acagttttat
     7321 tgccaccaca cataatttca tcgacaatct gaagaccggt ggacagcccc agctggatgg
     7381 accgaccgga aaagcggtgc tgcaatttac cctggctgcg ctgcaatccg cggcgaccgg
     7441 caaagttgta cggcctgatg atgtgttgta aagaggaagg atatatatga catattcact
     7501 gattccagtc cccattcacc tggtgttaat cgttttatac atgattgccc gcaatcgtaa
     7561 tgacttgaag aagacagcca ttatccaacc atctatcacc ttcctggcta tcgtgatcgc
     7621 agggttgagc tacttaagcc cgtatgtcaa ccctgcttac accacttgga tattggtcgg
     7681 cctggttttg tgttttattg gtgatatttt taacatcgat atgaccaatg ataagatttt
     7741 attcgccgct atggtcatct tctttattgc ctatgtggaa tacgcggttg cgttcacgcg
     7801 ttttggcggt ttccatactc aggattggct catcggcgcg ctgatgctcg ttgtttacat
     7861 tcttctaatc cggttgtatt ggaagggact gggaaatttc cgtatcccgg tgatgatctt
     7921 cgtgctggtc atcagcttta tggtcacgcg cgcgatttcc acgcttttca gcagtcaatt
     7981 ttcagcgctt tcagcctggc tggtcgcgtt tggcggtccg ctgcttttta tgggcgatgt
     8041 agaatatgga ttgcatcgct tccataagcc cttaaaattc catctcgggc cactctatta
     8101 cgccggcggt cagttattgg tcgcgctcag ttgcgcttac ttactctttc gctaaaggta
     8161 acgagttacg ttaagggtga aaacccgggt aaaatgaaaa actgaagcaa tgttattttg
     8221 aacatggtct aagaaggctt ctgctcgcag ctatggtcga ataaatatga aaacgcattt
     8281 cacctcctgg tattgaatga aaagcgattt aaaaggctgc ttaccaaaaa cctctaattg
     8341 tctggcaaac ctcgcaaatt ggccgaaacg aatgaaagcg gagattgtag gatttacggg
     8401 aaatatgatt gaaattctat agcgattatt tggtcaatta aaaatatcaa aattgccgtc
     8461 cagcttgcca tctgccatat ctgcgatgac aaaaccaccg aaaaatgggt ctggtgcacc
     8521 gggttcgtcc atgccccaaa gttcgatatc agtgtctgta tacccgtaat cgtaata
//
LOCUS       DAOSPM010000071         7690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_9504_length_7690_cov_13.357826, whole genome shotgun sequence.
ACCESSION   DAOSPM010000071 DAOSPM010000000
VERSION     DAOSPM010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9504_length_7690_cov_13.357826"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1184
                     /locus_tag="PLW25_06925"
     CDS             39..1184
                     /locus_tag="PLW25_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOS54256.1"
                     /translation="MTQAEIITIGTELLLGEILDTNTRYLAQQLRHLGIDLFRATSIG
                     DNPLRIAEMIRESLSRAEIVITTGGLGPTIDDPTRNAAAIAFNVEMDFHADLWKEIYR
                     KFQVRGAIPSDNNQKQAFLPANAVAIHNPVGTAPAFYIAEQKKLLVCLPGVPAEMKTI
                     FQHEVIPLLRGHFELHQLIKTHVIRTSGIGESVVDAQIADLETSSNPTVGLSAHPGCV
                     DIRITAKANSSQEADRMIDVVEQKVISLLQPHIYGFDQDTLLEKVIELAANRGVTVTL
                     FVFCAINELILFSNHTNNNVFEVKKLDRPYTPQQDLNLERRALLFAVNIYCQDDIATL
                     DMFFTSPQKHAEKKKLFNNALPQLQKWMENTCLDFIRRQLSEVENGE"
     gene            1186..3156
                     /locus_tag="PLW25_06930"
     CDS             1186..3156
                     /locus_tag="PLW25_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS54257.1"
                     /translation="MKTVDILLRNAEVLTMDHEFRRYECGALAINGKIIEAVGDEKEI
                     LAQYTAPQIVDCAGKILMPGLVNAHTHVPMTLLRGLADDLRLDVWLMGYMMPVEREFV
                     SPDFVRLGTQLACVEMTRSGITTFADMYYFEEEVAKATAEFGLRALCAQTVLKFPTPD
                     AQFYEESLELSRDFIQRWKSHDLIIPSVAPHAPYTCTAEILKATAALAAEFDVPLHTH
                     LSETAVEVENMRRENRMPVIPYVKKQGLFEAKVLAAHCVHVDDGEMHTLLHVNAGVAH
                     NPSSNMKLSSGVAPITRMLEIGLNVGIGTDGPASNNDLDMFEEMRLASFLQKGFSGDP
                     TTLPARTTLLMATRLGAQAMHIGHLTGSLEQGKRADLILIDIEPLHNSPRFQHDPNGT
                     YAQIVYAAKATDVKDVMVNGKWLMRDQQIIGLDEEDLLKHSRDYSRKIDQFLIQRESS
                     VLSKLVAIGGTSEEESFEIQLKIRVDDRNKLIEKLLHSDMEILRKRHYREFDIYFFFA
                     DINQGILRYREDEFLNEMGKIEQVRSRLTLIGPTSEDKFEQEVLLSRSRYLAPATQSP
                     RFYREYFKPHLAKEIEKDRIRYLVKFKDVEFFINFDEITKPPIGQFVEVKSRTWSRQD
                     AEMKSHLITELISFLGANLDEVTSDDYIEMVR"
     gene            3165..4661
                     /locus_tag="PLW25_06935"
     CDS             3165..4661
                     /locus_tag="PLW25_06935"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOS54258.1"
                     /translation="MGGESRSKKVIRVLFIASEAAPLVKAGGLGDVAGALPAALNRLG
                     AVVGGASAEVRLVIPFYNSIKTSNISATFIRKFKVSTLNGAVEAGLFETSLSGVVTYL
                     VSGAPVSESASIYGKDFKEDAEKFVFFSLACLKIPSLIGWKCDILHANDWHTAVAIHH
                     LATAKKKNASLQTTRSVLTIHNLPFMGSGVDEALVKYKISPSNDDNLPNWGKTQPLPM
                     GLAAADRIVAVSPTYAKEILTPEYGCDLQDFLRKRSGHLTGILNGIDVESWDPATDPA
                     LKFNFAVSNFTTRSQNKRALLAEFGFPVESDVPLLALVSRFDWQKGIDIIIAGLRAMK
                     NFAWRVILLGTGERNLEQACRDLEDEMPGKVRAAILFDVQLSRRIYAGADLLLMPSRY
                     EPCGLSQMYAMRYGCLPIARATGGLADTVSDPLSSTVPTGFLFPNAEASSFITALKRA
                     LRLYKKRPFWQAMQFNAMSRDFSWSVSARNYLDLYTQLSENSSIVSEP"
     gene            4658..6145
                     /gene="malQ"
                     /locus_tag="PLW25_06940"
     CDS             4658..6145
                     /gene="malQ"
                     /locus_tag="PLW25_06940"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOS54259.1"
                     /translation="MTLSRKSGILLHPTSLPGPDGIGDLGPECYRWMDFLHGCGCSLW
                     QILPLGPTGFGDSPYQCFSAFAGNPYLISPTLLLEERLLNRDDFLDRPNFPVEQVDFG
                     AAITWKTTILDRAFSRFKKSRSTKLHQQYQDFCRQEAGWLDDFGLFMAIKEVHGGGSW
                     DGWPVELRLRLPEALSKFSAENTPVVEKHKFRQFIFFSQWQRIFEYAHARGIQIIGDI
                     PIFIAYDSADAWANPELFFLGKDSRPTVVAGVPPDYFSPTGQLWGNPLYRWSAHKRQG
                     YAWWIKRFMSVLRTVDFARLDHFRGFAGFWEIPAGMPTAEIGRWVKGPGKNFLSAINR
                     TLKGLPIIAEDLGLITPDVIDLRDSFHLPGMKILQFAFGSTPEDPFLPHNYPFNCVAY
                     TGTHDNDTTRGWYESAPEIEKDLCRRYLASSGEHIHIDMIRELWKSPAAFAIGPLQDF
                     LGLGSESRMNYPGRPAGNWSWRYLPCQLSPELQRWVKELNFLYSR"
     gene            complement(6155..7129)
                     /locus_tag="PLW25_06945"
     CDS             complement(6155..7129)
                     /locus_tag="PLW25_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOS54260.1"
                     /translation="MREVIPDKQVDNRAKYLAGLFAGILAVSTASIFIRFAQQEVPSL
                     VIAAGRMTFATLLMLPFGLAELKRNPTAFNKKQIFHLGFAGAALGMHFAAWITSLEYT
                     SIASSVVLVTTAPLWVALVSPALLKERLTKPVVIGLLIALVGGIIVGGSGSCSTVQGK
                     FTCSGIGDLFAGSHSLGNLLAFAGALLSGAYLIAGRKVRNTISLPLYTLIVYGIAAFL
                     LSMMVVLKGDKVTGYATISYVWVLALALIPQAVGHTAFNWALKYLPAAFVSIALLGEP
                     VGTIILAALLLHEAPTFLEMMGGLLVLTGIAVATRKAAPKKIEYEKSQ"
     gene            7264..>7690
                     /locus_tag="PLW25_06950"
     CDS             7264..>7690
                     /locus_tag="PLW25_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOS54261.1"
                     /translation="MPFELPALPYAYDALEPVIDARTVEIHYSKHHATYLKNLNAALE
                     KYPQFYEWPLKKILGSLDQIPEEIRTAVRNNGGGVYNHNVYWFCLSPGGSSAPAGKLK
                     EALEKKFGDFATFKSEFEKAGLTRFGSGYAWLSAKPGGEL"
BASE COUNT         1977 a         2002 c         1826 g         1885 t
ORIGIN      
        1 gttaaaacct gataaaatga tttattcaac ggagtgagat gacacaggct gaaatcatca
       61 cgattggaac cgaactcctg ctgggcgaga tcctggacac caatacacgg tacctggcac
      121 agcagttgcg ccatctcggg attgatttat ttcgcgcgac atcgattggc gataacccgc
      181 tgcgcatcgc tgaaatgata cgagaaagcc tctcgcgggc ggaaatcgtc atcaccaccg
      241 gcggtctcgg cccgaccatt gacgatccta cccgtaatgc cgcggcgatt gcttttaacg
      301 tggagatgga ttttcacgca gacttatgga aagaaatcta ccggaaattc caggttagag
      361 gtgccattcc ctctgacaat aaccagaaac aggcgttcct cccggcgaac gcagtcgcca
      421 ttcacaaccc ggttggaacc gcacctgcgt tttatatcgc ggagcaaaag aaactgctgg
      481 tttgcctgcc gggagtgccc gcagaaatga aaacgatttt tcagcatgaa gtgataccgc
      541 tcctgcgcgg gcacttcgaa cttcaccaac tcatcaagac ccacgtcatc cgcaccagcg
      601 ggattggtga atcggtggtt gacgctcaga ttgccgatct ggaaacatcc agtaacccaa
      661 cagtgggcct ttccgctcat cctggatgtg tggatattcg catcactgcc aaagcaaatt
      721 catcccaaga agcggatagg atgattgatg tcgtcgagca gaaggtcatc tctctgcttc
      781 aaccgcacat ttacggtttt gatcaggata cacttttgga gaaagtcatt gaactcgcag
      841 ccaaccgtgg ggtaacggtt actttattcg ttttttgcgc gatcaacgaa ttgattttat
      901 tttcaaacca caccaacaat aacgtttttg aagttaagaa acttgaccgc ccttataccc
      961 ctcaacaaga cctcaacttg gaaaggcgcg cgttattatt tgccgtaaac atctattgcc
     1021 aggatgatat agcaaccctg gacatgtttt ttaccagccc tcaaaaacat gcggaaaaaa
     1081 agaaactgtt caacaacgct ttaccgcaac tgcaaaaatg gatggaaaac acctgcctgg
     1141 atttcatcag aaggcaactt tctgaggtcg agaatggaga ataacatgaa gacagtcgat
     1201 atcctgcttc gcaatgccga agtgctcacc atggaccatg aatttcgccg ttatgaatgc
     1261 ggagcgctcg ccatcaacgg aaagattatc gaagctgttg gcgacgaaaa agaaattctc
     1321 gcccagtata cagccccgca aatagtagat tgcgcgggta aaattttaat gcccggtctg
     1381 gttaacgcgc acacgcatgt acctatgacc ctgctgcgtg ggcttgcgga tgacctgcgt
     1441 ttggatgttt ggctcatggg gtacatgatg cctgttgagc gagaatttgt ttctcctgac
     1501 ttcgtgcgtt taggtacaca gctcgcttgc gtcgaaatga cccgttcagg aatcacgaca
     1561 ttcgccgata tgtattactt tgaagaagaa gtagccaaag cgaccgccga gtttggcttg
     1621 cgcgcgcttt gcgctcaaac cgtgcttaaa tttccaaccc ctgatgctca gttttacgaa
     1681 gaatctcttg aattgtcacg cgattttatc caacgttgga aaagccatga tctgatcatt
     1741 ccttccgtgg cacctcacgc tccgtacacc tgtacggccg aaatccttaa agccaccgcc
     1801 gccctggctg ccgaatttga tgtccctttg catacgcacc tgtctgaaac ggctgttgaa
     1861 gttgaaaaca tgcgccgaga gaaccgaatg ccggttattc cttatgtaaa aaaacaaggt
     1921 cttttcgaag cgaaggtcct tgctgcccat tgtgtgcatg tggacgacgg tgaaatgcac
     1981 accctgctgc atgtgaacgc gggtgtggcg cacaaccctt cctctaacat gaagttgtct
     2041 tcaggcgttg ccccaatcac ccgcatgctg gagattggcc ttaatgtggg tattggcacc
     2101 gatggtccag cttccaacaa tgacctggac atgtttgaag aaatgcgcct ggcttctttt
     2161 ctgcaaaaag ggtttagtgg agacccaaca accttgcccg ccaggaccac gctgctgatg
     2221 gcaacacgcc ttggagccca ggccatgcac atcggtcatc tgacgggttc tctggagcaa
     2281 ggaaaacgag cagaccttat cctgatcgat attgagccgc tgcataacag ccccagattt
     2341 caacatgacc cgaacggcac ctacgcgcag atcgtctacg cggctaaagc cacagatgtg
     2401 aaagatgtga tggttaacgg caaatggctg atgcgcgatc aacagatcat cggtctggat
     2461 gaagaagatt tattgaagca cagccgcgat tattcgcgca agatcgacca atttctcatc
     2521 caacgagagt cttctgtcct ctccaagctg gttgccatcg gtggtacatc tgaagaagaa
     2581 agctttgaaa ttcagttaaa aattcgcgtc gacgaccgca acaaattgat tgaaaaatta
     2641 ctgcattcag acatggagat tcttcgaaaa cgccattacc gtgaatttga tatttacttc
     2701 ttcttcgcgg atatcaacca gggaatactg cgctaccgtg aagacgagtt ccttaacgaa
     2761 atgggaaaga tcgagcaggt tcgatccagg cttaccttga tcggtccaac cagtgaagat
     2821 aagttcgaac aggaagtttt actgtcacgc agccgctacc tggctccagc cacacaaagt
     2881 ccgcgctttt accgggagta tttcaaaccg cacctggcca aagagattga aaaagaccga
     2941 atccgctatc tggtgaaatt caaggatgta gaattcttta tcaattttga tgaaatcacc
     3001 aaaccaccca ttggtcaatt tgttgaggtg aaaagtcgca cctggagccg tcaggacgct
     3061 gagatgaagt ctcacctcat tactgaactg atcagctttt taggcgccaa cctggacgaa
     3121 gtcacttctg acgattatat tgagatggtc aggtaaacag ataaatgggc ggtgagagtc
     3181 gatcgaaaaa agtgatccga gtgcttttca tcgcttctga ggctgctcca cttgttaaag
     3241 ccggggggct aggagatgtg gctggcgctt taccagccgc gctcaatcgt ctgggtgcag
     3301 ttgtgggggg agcatcagca gaagtgcgcc tggtgatccc tttctacaac tcgatcaaga
     3361 ccagtaatat ttcagcaacc ttcattcgaa aattcaaagt cagcaccttg aatggcgcag
     3421 tggaagccgg attatttgaa acctcccttt caggagtggt cacctatctg gtttccggcg
     3481 caccggtgag cgaatccgct tcgatctatg gaaaggactt taaagaggac gctgaaaagt
     3541 tcgtcttttt ctctctcgcc tgcttaaaaa tcccttcctt gatcggttgg aagtgtgaca
     3601 tactgcacgc caatgattgg catacagcag tggcaataca tcacctagcc acagcgaaga
     3661 agaaaaacgc ctccctgcaa acaacgcgct cggtgctgac aatacacaac ctgcctttta
     3721 tgggaagcgg ggtggatgaa gccctggtga aatacaaaat ttccccatcc aatgatgaca
     3781 acctgccaaa ttgggggaaa acccaacctc ttccaatggg tttggccgcc gctgaccgaa
     3841 tcgtggcggt ctccccgacc tacgcaaaag aaatcctcac gccggagtat ggttgtgatt
     3901 tacaggactt tcttagaaaa agatccggcc atctcactgg tattcttaac ggcattgatg
     3961 tcgaatcctg ggatccggcg acagaccccg cacttaaatt caactttgct gtcagtaatt
     4021 tcaccacacg cagccaaaat aaacgggcct tgctggctga attcggtttt cctgttgaaa
     4081 gcgatgtccc cctgctggct ctcgtcagca gatttgactg gcaaaaaggg atagatatca
     4141 ttatcgccgg tcttcgcgcc atgaaaaatt tcgcctggcg cgtcatttta cttggaaccg
     4201 gggagcgcaa ccttgaacag gcctgccgag accttgaaga tgaaatgccg gggaaagttc
     4261 gcgctgccat tctttttgat gtccagctct ccaggcgtat ttatgcgggc gctgatcttt
     4321 tactcatgcc ctcccgttat gaaccctgcg gcctttcaca aatgtatgcc atgcgctatg
     4381 gctgcctgcc aattgccaga gccaccggcg ggctggctga cacggtttct gacccgcttt
     4441 catccacagt accaacaggt tttctcttcc ccaacgctga ggccagttct ttcatcacgg
     4501 ccttaaaacg cgccttgcgc ctgtataaga aacgcccttt ctggcaggca atgcagttca
     4561 acgcgatgtc gcgtgacttc tcctggtctg tctctgcgcg taactatctt gatctctaca
     4621 cgcagttgtc cgaaaattca tctattgtga gtgaaccatg accctttcgc gaaaatctgg
     4681 tatcttgctg cacccaacca gtctgccagg ccctgacggt attggtgatc tggggccgga
     4741 gtgttatcgc tggatggatt tcctgcacgg ctgtggttgt tccctatggc agattctccc
     4801 ccttggccct acaggttttg gcgattcacc ttaccagtgc ttcagcgcct tcgccggtaa
     4861 cccttattta atttcaccaa ctttattgct tgaagagcgc cttcttaacc gggatgattt
     4921 tctcgacaga cccaatttcc cagttgagca ggtagatttc ggcgctgcca tcacctggaa
     4981 aaccaccatc cttgaccggg cttttagcag atttaagaaa tcacgttcaa cgaagctgca
     5041 ccagcaatat caagatttct gcaggcaaga agcgggctgg cttgacgact ttggtctttt
     5101 catggcgatc aaggaagttc atggcggagg ctcgtgggat ggctggcccg tagaactccg
     5161 cttgcgcctt cccgaagccc tttcaaaatt ctctgccgaa aatacgcctg ttgtggaaaa
     5221 gcacaagttc aggcaattca ttttcttttc tcaatggcag cgaatttttg aatacgcgca
     5281 cgcgcgcgga attcaaatca tcggtgatat ccccattttt attgcctacg acagcgcgga
     5341 tgcctgggca aaccccgaac ttttctttct gggcaaagat agccgcccga ctgtcgtcgc
     5401 aggtgtcccg cctgattatt tttctcctac cggtcagctg tggggcaacc cactatacag
     5461 atggtctgct cacaaacgcc agggctacgc ctggtggatc aaacgcttta tgtcggtgct
     5521 gagaacagtg gatttcgccc gcctggatca ttttcgcggg ttcgcgggtt tttgggaaat
     5581 acctgccggc atgccaaccg ctgaaattgg gcgttgggta aaaggccctg ggaaaaactt
     5641 cctatcagcc attaacagga ctttaaaagg cttacctatc atcgccgaag acctggggct
     5701 gatcacgcct gacgtgatcg acctgcgtga ctccttccat cttccaggga tgaaaattct
     5761 ccagtttgct tttggtagca cgcctgaaga cccctttttg ccacacaact acccgttcaa
     5821 ctgcgtggct tacaccggaa cccatgacaa cgataccaca agaggttggt atgaaagcgc
     5881 cccagaaatt gaaaaagacc tgtgccgcag atacctggcc agctctggag aacatattca
     5941 tatagacatg atacgcgagc tatggaaatc accagcggca ttcgctatcg gtcctcttca
     6001 ggattttcta gggttgggca gcgaatcccg catgaactat cctggccgcc ccgcaggtaa
     6061 ctggagctgg cgttacctgc cttgccaact gagccccgag ttgcaacgct gggtaaaaga
     6121 attaaacttc ctttacagcc gttaacagct caatctactg gcttttctca tattcaattt
     6181 tcttgggtgc tgctttccgg gttgccacgg caattccagt tagcaccagt agcccaccca
     6241 tcatttccaa aaaggtcggc gcttcgtgca gcagcagggc tgccaggatt atcgttccca
     6301 ccggttctcc aagcagcgca atagaaacaa atgcggcagg cagatacttg agcgcccagt
     6361 tgaaggccgt gtgcccaacc gcctgtggaa taagcgccaa tgccagaacc catacataac
     6421 taatagtcgc ataacctgtc accttgtctc ctttgagcac caccatcatg cttaaaagga
     6481 acgcggcgat accatagaca atcaaagtat aaagaggcag cgaaattgta tttctaacct
     6541 ttctgcccgc aatcaggtaa gcgcctgaaa gcagcgctcc ggcaaaagcc aatagattgc
     6601 caagtgaatg cgaaccagca aaaaggtcac cgatgccgct gcaggtgaat tttccttgaa
     6661 ccgttgaaca gctgccactg ccgcccacaa tgatcccacc gacaagagcg atcaataacc
     6721 caataaccac tggtttggtt aatctctctt ttaggagcgc tggtgaaacc agcgctaccc
     6781 ataacggcgc agttgtgacc aacacaactg aactggcgat tgacgtatat tcaagcgacg
     6841 tgatccaggc ggcaaagtgc attcccagcg cggctccggc aaaaccaagg tggaaaattt
     6901 gctttttatt gaaggctgtc gggtttcgtt ttaattctgc cagtccgaag ggcagcatca
     6961 acagcgtagc gaaggtcatc cttcctgccg cgatgacaag agagggtact tcctgctgtg
     7021 cgaaacgaat aaagatagaa gcagttgaaa ctgccaaaat gccagcgaat aaacccgcca
     7081 ggtatttggc gcggttgtca acctgtttat caggaatcac ttctctcata ccctgtcaag
     7141 aaatacgttt tccagttaca gtgtgttttc ttcacttctg cttgacaatc cttcatctga
     7201 tggctagaat tttaactgaa atgctcgcaa aatacatacg aaaccaaccg atgggaggga
     7261 aaaatgccgt tcgaactgcc cgcacttccg tatgcgtacg acgcgcttga acccgtaatt
     7321 gatgccagaa ccgtagaaat acattacagt aaacaccatg cgacctactt gaaaaacctc
     7381 aacgccgcgc tggagaaata tcctcaattt tatgaatggc cgcttaaaaa gattcttggc
     7441 agccttgacc agattcctga agagattcgt accgccgtca gaaacaacgg tggtggagta
     7501 tataatcata atgtctactg gttctgccta tcacctggcg gctccagcgc gcctgctggc
     7561 aaattaaagg aagcattgga aaaaaagttc ggtgattttg ccactttcaa gtctgagttt
     7621 gaaaaagccg ggctaacccg ttttggcagc gggtatgcct ggttgagcgc caaaccaggt
     7681 ggtgaacttg
//
LOCUS       DAOSPM010000072         7636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_9592_length_7636_cov_15.914391, whole genome shotgun sequence.
ACCESSION   DAOSPM010000072 DAOSPM010000000
VERSION     DAOSPM010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9592_length_7636_cov_15.914391"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..2037
                     /locus_tag="PLW25_06955"
     CDS             202..2037
                     /locus_tag="PLW25_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOS54262.1"
                     /translation="MTVTSIEPKDNKEKWLARLRRFFRWLTSPHVVLSIIMVAVMFYL
                     VIIPLYRMVVTTITVADVDLRAIRGAEVGDFTWYHWVRMLTGKIGKLMTYQPAMHSIV
                     VALGATFLALAVGGLMAWLVCRTDIPGRDTIHMLAQVPYIMPSWTIAMAWLVLFKNQK
                     SGGTMGLLEFITGAPTPDWLAYGPIPIIVSSGLHYYTFFFLFIAAALLSIDSSLEEAG
                     ELAGAGRWRIMRKITFPLVIPAIASGFIMTFSKAMGTFGGPNVLGVPVRYYTLSTMLR
                     SNSSIGNYGDAFVTAIVLILFAMVTVMLNQKIVGTRKSYETIGGRGFMSKKTKLRNFK
                     PIIVAIVVIFQFIIAVLPLVLLVYSTFMLRDGKWTLDNFTLAHWIGKGDLSINSGEPG
                     ILLNHKVWLGAWNSVRLSILTALFTALFGVVLGYAIVKGRGTWLSKIVEQMSFIPYVI
                     PGIAFGAVYIAMFTKPVGPIPALYGTFALLVVVSVAKHLPFSARSGVSAMMQVSKELE
                     EAAMLAGASPWRRFARIVFPLTSTGFVSGFLLTFITTMRELSLIILLVTPTTAVLASM
                     TMRYIENGNDQQANAVIILLIVLVLIGNFIISRFRGGSLKKGLGL"
     gene            2050..3135
                     /locus_tag="PLW25_06960"
     CDS             2050..3135
                     /locus_tag="PLW25_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS54263.1"
                     /translation="MDTPIVINNLTKKFGDVIAVNNVSLRIEPHTFLTLLGPSGCGKT
                     TLLRCISGLEDPDEGEIYIGDTLIYSHAKGISVPPGQRELGMVFQNYALWPHMTVYKN
                     MTFALEIQKLPKAEMDKRVKESLEEVKMSGYENRYPREMSGGQQQRIALARMLAYRPK
                     VFLMDEPLSNLDARLRMDMRTELKRLHHISDATTVYVTHDQLEAMTMSTNIAVMKEGV
                     IQQMDTPDMIYHYPANLFVADFIGNPKINLLKGWVSSEDVVDLGKFNIPAYTHRKTGD
                     VVAAFRPEDITIHTEPVEGGVEFSAYSVQPSGADTTIIAHKGDTEIIVKEMGVSKIAM
                     DQKIWLTFNKEAINLYDKATEKLITGS"
     gene            3237..4469
                     /locus_tag="PLW25_06965"
     CDS             3237..4469
                     /locus_tag="PLW25_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS54264.1"
                     /translation="MKAVIRIATLVLLVSFLAACAKPTAAPVVEPPVVEEPVVTEAPV
                     VEELTAQQQWLKDNQLGEYYTDEQDWAAIEAAAIAEGEVLVYANTSKIEKSAKAFMEK
                     YPGINVQAFDLGGDDVLLKTVEEQKAGAFTGDVWSSSGGAELVGNVLPKGYVWRFIPS
                     TVADRIPEQYTHPLLMARLGTAVFAYNSELNDTCPITNIWELTQPEWKNKFFIEDPIN
                     DASTLSKLLTLVSHSEEMAAAYVELYGTEPVLEEDTPDAGWLWLKKFAQNGPIPQPGG
                     DEVDEAFATPGMKESYMAITSYSNMADVMEGNLVFEPCWGIKPILGIQSQSYLGIINQ
                     APHPNAAKLWIAWQVSEEGRKPWAKFGTYFTDSTYEVPEGQKPLSEMMAVTWFIDEQF
                     AYDHMIEARDFYLINLAE"
     gene            4613..6043
                     /locus_tag="PLW25_06970"
     CDS             4613..6043
                     /locus_tag="PLW25_06970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOS54265.1"
                     /translation="MALRTMLFDVGGTLETYSCSRELRIKNTAVFRNFLKENGIILPY
                     SDEQLYEVISSGISAYRKWSIASMIELSTEEIWSQYVFRGHPVDQEKLNRISEQLSCL
                     YELNYYVREMRPEVPAVLAEIKKMGFRIGCISNTQSLTQVPATLEKYGISQYFDPVVL
                     SSKYGRRKPDPAIFYHAARLANSPTGACGYVGDKINRDVLGAKKAGFKLAIQVRHEFD
                     DGEVDEGAEPDAIIENIADLLPLLEASIRENRSISREDERSRIKAVFFDAGDILYYRP
                     QRDKYLNEFLSGHQLNPVPGFEDERKRIKDLAFSGQMRRHDYYRELIRLYGITDEQAI
                     EQGMEALSRDDDTVAIFDGVPETVHTLKERGFILGIITDTAMPITRKLNWFEKHGFGR
                     VWDVVISSKEMGVRKPSPSMYEEALQQTGIRSQEAVFVGHKTHELEGARAVGLKTIAF
                     NYDPDAPADYYIEHFQDLLKVPLLAN"
     gene            6070..>7636
                     /locus_tag="PLW25_06975"
     CDS             6070..>7636
                     /locus_tag="PLW25_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOS54266.1"
                     /translation="MQRKVKAIFFDIGGTLRVTHPEEGRDMSKIQALMSLIGEKSDPL
                     DFVARVHKGEKAYRRWCKPNYIELSEAELWTRFLLPEYPAPFIRENAITINQFWRESK
                     PKYVLPDMVETMRELSKRGYRLGLISNTTSSVEGYQLLEETGLTRLFSSVILSAEFGR
                     RKPHPSLFIEAARRAGVRADECAYVGDRPSRDLIGARQSAYAEVVIINSAGYSPDEYD
                     PDDYDPEKDTHLTLRPDHWIKRLSELLEIYKPIKTAAQENPQKESEYKLFDVALSTMW
                     HVGQAASFNETFELVRTIGISRFEFNHQVTPQLLATFDCNRNYISTVHDPCPAPRDLR
                     ALKNDDILISSLNEARRGQAVDLVKRTLDLAISLGSRSMVLHTGSLQCDRARDRRLRQ
                     MYEEGQRGSEEFCQLREEMIAHRAQYAPAHLDAVKRSIAEIITFAKGSGVNIGVENRY
                     RYYDLPLPSEMQEILDLCHESWFGFQLDTGHAHALETLGLCEKGVWLQRFHNRIIGVH
                     LHDVAGLTDHQVPG"
BASE COUNT         1921 a         1867 c         2037 g         1811 t
ORIGIN      
        1 atgacggtga tcaacgcgct gctgctttca gttgccagtt tagaccagga gcggattatg
       61 ccagcactgg ataaattgga ttccctgcgc ggtcagctga aggaattaaa ccgggcagga
      121 tgattcgttt gtgtgaatct tacggcatga ttcatgttca acacttgatg aagaaaggaa
      181 atcaacacaa ggtgcaagct gatgacagta acatcaattg aaccaaagga caacaaggaa
      241 aagtggctgg ctcgcctgcg tcgctttttc cgctggttga cctcgccgca tgtggttctt
      301 tccatcatca tggttgcggt gatgttctac ctggtcatca taccgcttta tcgcatggtc
      361 gtcactacta tcacggtagc ggatgttgac ctgagggcga tcagaggtgc tgaagtaggc
      421 gactttacct ggtatcactg ggtgagaatg ctcacaggca agatcggcaa actgatgact
      481 tatcaaccag cgatgcatag catagtggtt gccctgggag caaccttcct ggcgttagcg
      541 gttggcgggt tgatggcgtg gctggtgtgc cgcacagata tcccgggacg cgataccata
      601 catatgctgg cgcaggtgcc ctatatcatg ccttcgtgga ccatcgcgat ggcctggctg
      661 gtgctcttta agaaccagaa atcagggggc accatggggc tgctggaatt tattactggc
      721 gcgcccacac cagactggtt ggcgtatggc ccaattccca tcattgtgag cagcgggttg
      781 cattactaca ccttcttttt cctgttcatc gcggcggcgc tcctgtcgat tgattcatcg
      841 cttgaagaag cgggcgagct ggcgggagcg gggcgctggc gcattatgcg gaaaattacc
      901 ttcccgctgg ttatccctgc tatcgcatca ggtttcatta tgaccttttc gaaggccatg
      961 ggcacctttg gcggaccgaa cgtcctgggg gtgcctgtcc gttattacac cctttcgacc
     1021 atgttgagat ccaattcttc catcggcaac tatggcgacg cctttgtgac tgccattgtg
     1081 ctgatcttgt ttgccatggt caccgtgatg ttaaatcaaa agatcgtggg cacgcgaaaa
     1141 agttatgaaa cgatcggtgg tcgtgggttt atgtcgaaga agaccaagtt gagaaatttt
     1201 aagccaatta tcgtcgctat cgtggtcatc tttcagttca ttatcgctgt gctgcccctg
     1261 gtgctgctgg tctacagcac attcatgctg agagatggaa aatggacgct ggacaacttc
     1321 actctcgctc actggattgg aaagggcgat ctctcgatca acagcggtga accgggcatc
     1381 ctgctcaatc acaaggtttg gttgggtgcc tggaactccg ttcgtttgtc catccttacc
     1441 gccctcttca ccgcgctttt tggagtggtg ttagggtacg cgattgtcaa agggcgcgga
     1501 acctggcttt cgaaaatcgt ggaacagatg tcctttatcc cttacgtcat cccgggcatc
     1561 gcttttggcg cggtatacat cgccatgttc accaaacctg ttggccccat cccggcgctg
     1621 tacggcacct tcgcgttgtt ggtggtggtt tcggtagcca agcacctgcc tttctcagcg
     1681 cgcagcggtg tttctgccat gatgcaggtg agcaaagaac tggaagaggc agcaatgctg
     1741 gctggtgcgt ctccctggag gcgtttcgcg cgcatcgtct ttccgctcac cagcaccggt
     1801 tttgtttctg gttttctact gacattcatc accacgatgc gcgaactctc tctcattatc
     1861 ctgctggtca cccccaccac agcagtgctg gcgagcatga ccatgcggta cattgaaaat
     1921 ggcaacgacc aacaggcgaa tgccgtcatt atcttgttga tcgtgctggt actgatcggc
     1981 aactttatta ttagccgctt ccgtggcggc agtctgaaaa aagggctggg actgtaaaga
     2041 ggagaagaaa tggatacccc aatcgtcatt aataacctca ccaagaagtt tggcgatgtc
     2101 atcgccgtca ataatgtttc attgcgtatt gaacctcaca catttttaac gctgctcggt
     2161 ccttctggct gcggtaaaac caccctgctg cgctgtattt ccggtcttga agacccggat
     2221 gagggcgaga tatatattgg tgatacgctt atctattctc atgcgaaagg gatatctgtg
     2281 ccgcccgggc agcgggaact gggaatggtc tttcagaact acgctttgtg gccgcacatg
     2341 acggtgtata aaaacatgac cttcgctttg gaaattcaga aactgcccaa ggctgaaatg
     2401 gacaagcgcg tgaaagaatc acttgaagag gtgaagatga gcgggtatga aaaccgctac
     2461 ccgcgtgaga tgagcggcgg tcaacagcag cgtattgcgc tggcgcgtat gctggcttac
     2521 cgcccgaagg tctttttgat ggatgaacct ctttccaatc tggacgctcg tttgcgtatg
     2581 gacatgcgca ccgagctgaa acgcctgcac catatttcag acgccaccac cgtgtacgtg
     2641 acacatgacc aactggaagc catgaccatg tccaccaaca tcgccgtcat gaaagaaggg
     2701 gtcattcaac aaatggacac gccagacatg atctatcatt accccgccaa cctgtttgtg
     2761 gctgatttta tcggtaaccc gaagatcaac cttctgaaag gctgggtttc gagtgaggat
     2821 gtggttgacc tgggtaagtt caacatcccc gcgtatacac acagaaagac cggtgatgtg
     2881 gtggcagctt tccgcccgga ggacatcact atccacactg agccggttga aggtggggtg
     2941 gaattcagcg cttactctgt acaaccatcc ggtgccgata ccacgatcat cgcgcacaaa
     3001 ggcgataccg agatcattgt caaagaaatg ggtgtcagta agattgccat ggaccagaag
     3061 atctggttga cctttaataa agaggccatt aacctttatg ataaggcgac agaaaaattg
     3121 ataacaggtt cgtgaattaa tgtcaggtga aataaaaaaa ggaggaggtg atcgtatcgt
     3181 cttttggtgt cgttttctgt ctgttgaatg ttcaaaataa acaaggagga aggtcaatga
     3241 aagcagtaat tcgaattgca acacttgtat tgttggttag cttcctggcg gcctgcgcga
     3301 aacctactgc ggctcccgtg gttgaaccgc cggtggttga agaacccgtc gtaacggaag
     3361 cccctgtggt ggaggaattg acagcccaac agcagtggtt gaaagacaac cagcttggcg
     3421 aatactacac cgacgaacag gactgggcgg ccatagaggc ggcggcgatt gcagaaggtg
     3481 aagtgctggt gtacgcgaac acatccaaaa ttgaaaaatc ggcaaaagcc ttcatggaaa
     3541 aataccccgg gatcaatgtg caggcgtttg acctgggcgg agatgatgtg ctgctaaaaa
     3601 cggtagaaga acagaaggct ggcgccttca ctggtgacgt gtggtcaagc agcggtgggg
     3661 ctgaattggt tgggaatgtg ttgcctaagg gttatgtatg gcgatttatc ccctccactg
     3721 ttgcggatag gattccagag caatatactc atcctctctt gatggcacga ttgggcaccg
     3781 ccgtgttcgc gtacaactca gaattaaacg atacatgccc catcaccaac atctgggagc
     3841 tcacacaacc tgaatggaag aacaagttct tcattgaaga cccgatcaac gatgcttcaa
     3901 cactcagcaa actcctcacc ctggtaagtc attctgaaga aatggccgca gcctatgttg
     3961 agctgtatgg caccgagcca gttctggaag aggatactcc tgacgcgggt tggctgtggc
     4021 tgaaaaaatt cgctcagaat ggaccaatcc cgcagccagg cggcgatgag gtggatgagg
     4081 cgttcgctac tcctggaatg aaagaaagct atatggccat cacttcctac tccaacatgg
     4141 ccgatgtgat ggaaggcaac ctggtatttg aaccctgttg gggaatcaaa cccatccttg
     4201 gtattcagag ccagagttac ctgggcatca tcaaccaggc gccccacccc aacgcggcca
     4261 aactttggat cgcctggcag gtgagcgaag aaggccgcaa accctgggcg aaattcggaa
     4321 cttacttcac agattccact tatgaagttc ctgaagggca aaaaccgcta tctgagatga
     4381 tggcagtgac ctggttcatt gacgaacaat tcgcctacga tcacatgatc gaagcccgcg
     4441 acttctactt aatcaatctg gcagagtaac acacctatcc ttgttccacg gcacggacct
     4501 tcgcttacag gtccgtgcct ggaacctggg cgtagaaaga aaattcaaac tggaagaaca
     4561 gtaccctttc ctgaactcgg gaaggtgaca taaccacacc ctggagagtg ttatggcgct
     4621 gagaaccatg ctttttgatg tcggcggaac gcttgaaacc tacagttgta gcagagaatt
     4681 acgaattaaa aataccgccg tcttcagaaa ttttttgaaa gagaatggca ttattctccc
     4741 gtatagcgat gagcaactat acgaagtgat ctcgagcggt ataagcgcct accgcaagtg
     4801 gagcatcgcg tcgatgattg agctcagcac ggaggaaatc tggtcacaat acgttttcag
     4861 gggacacccg gttgaccagg aaaagcttaa tcggatttct gagcaattat cctgtcttta
     4921 tgaactgaat tattacgtgc gtgaaatgcg gccggaagtt cccgcggttt tggctgaaat
     4981 taaaaagatg ggcttccgaa tcggctgcat cagcaacacg caaagcctca cccaggttcc
     5041 agcaacgctg gaaaaatacg gcatcagcca atattttgac ccggttgtgc tttcctcaaa
     5101 gtatggcagg cgtaaacctg accccgcgat tttttatcat gcggcgcggt tggcgaactc
     5161 ccctaccggc gcctgtgggt acgtggggga taaaatcaat cgagatgtac tgggcgcaaa
     5221 aaaagcaggt ttcaaactcg ccatccaggt gcggcatgaa ttcgatgacg gtgaagtcga
     5281 tgaaggagca gagccagacg caattattga gaacatcgcc gacctgcttc cattgttaga
     5341 agcctcgatt cgtgaaaacc gttctatcag ccgggaagat gagcgctcgc ggataaaggc
     5401 ggtatttttt gatgccggag atattttata ctaccgccca cagcgtgata aatacttaaa
     5461 tgaattctta tctggtcatc aactaaaccc tgtgccaggt tttgaggatg agcgtaaaag
     5521 gattaaggat ctggcttttt ctggtcagat gaggcggcac gactattacc gtgaactgat
     5581 ccggctatat ggtattaccg atgagcaggc tattgaacag ggtatggaag ccctcagccg
     5641 tgatgacgac acagtggcca tctttgatgg cgttcctgaa acggttcaca cgctgaaaga
     5701 aagagggttt attctgggaa tcatcaccga tacagccatg ccgataaccc gcaaactcaa
     5761 ctggtttgaa aaacacggtt tcggccgggt ttgggacgtg gtgatcagct ctaaggaaat
     5821 gggggtgcgc aaaccctccc ccagtatgta tgaagaagcc ctccaacaaa ccggtatccg
     5881 ctctcaagaa gcggtatttg tggggcataa aacccacgaa cttgaaggcg ccagggctgt
     5941 agggttaaag accatcgcct ttaattatga cccagatgcg ccggctgatt attacattga
     6001 acattttcaa gacctgctga aggtgccttt attggcgaac taatccataa cgaatgaata
     6061 aggaaacgaa tgcaaagaaa agtaaaagcg atcttttttg atatcggcgg aacattacgc
     6121 gtgacacacc ctgaagaggg cagggatatg tcaaagattc aggctttaat gagcctgatc
     6181 ggagaaaagt cagacccttt ggactttgtc gctcgtgtgc ataaaggtga aaaagcctac
     6241 cgacgctggt gtaaaccaaa ttacattgaa ctgtcggagg cagaattatg gacacggttt
     6301 ttattgccag aatacccggc gccatttatc cgtgaaaacg ctattaccat caaccaattc
     6361 tggcgtgaat caaagccaaa atacgtgctt ccggatatgg tggagaccat gcgggagctc
     6421 tccaagcggg gctacaggct tgggcttatc agcaacacca cctcctctgt agagggttac
     6481 cagctgctgg aagaaaccgg gttgaccagg cttttcagca gcgtaattct ttcagcggag
     6541 ttcggcaggc gaaaacctca cccttccctc ttcatcgaag ccgcgcggag ggcaggcgtg
     6601 agagcggatg aatgcgccta tgtgggtgac cgcccttcgc gcgaccttat tggagcgcgc
     6661 caatccgcct acgccgaagt ggtcatcatc aatagcgcgg gttacagccc ggatgagtat
     6721 gacccggatg attatgaccc agagaaagat actcatctca ccttgcgccc tgatcattgg
     6781 atcaaacgcc tttcggaact cctggagatc tacaagccca taaagaccgc tgcacaggag
     6841 aacccacaaa aggaatcgga gtataaactt ttcgatgtgg cgctctcgac catgtggcac
     6901 gttggtcaag cggcatcttt taatgaaacc tttgagcttg tgcgaacgat cggcatctca
     6961 cggtttgaat tcaatcacca ggtcactcca cagctgctgg caacgtttga ctgcaaccgc
     7021 aattacatca gcacggtgca cgacccctgc ccggcgccgc gtgatttaag agccctgaaa
     7081 aatgacgaca ttttaatctc gagcctgaat gaagcgcgcc gggggcaagc agtcgacctg
     7141 gttaaacgca cgcttgacct ggcaatttcg ctggggtcgc gctccatggt gctgcacacg
     7201 ggctcgttgc agtgtgatcg tgcgcgcgac cgccggctgc gccagatgta tgaggagggg
     7261 cagcgtggtt ctgaggagtt ctgccagcta agagaagaga tgattgccca ccgcgcgcaa
     7321 tacgcgccag cgcatttaga cgcggtcaag aggagtatcg ctgagattat caccttcgcg
     7381 aaaggcagcg gcgtgaacat cggcgttgag aaccgctatc gttattatga cctgccgctt
     7441 ccaagtgaaa tgcaggagat cttagattta tgccatgagt cctggttcgg ttttcaactg
     7501 gatactgggc atgcgcacgc gctcgaaacc ctgggtttat gtgaaaaagg agtgtggctg
     7561 cagcgatttc acaaccgtat catcggagtt cacctgcacg atgtggctgg gttaaccgac
     7621 caccaggtgc ccggca
//
LOCUS       DAOSPM010000073         7492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_9858_length_7492_cov_12.300928, whole genome shotgun sequence.
ACCESSION   DAOSPM010000073 DAOSPM010000000
VERSION     DAOSPM010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9858_length_7492_cov_12.300928"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="PLW25_06980"
     CDS             complement(<1..1589)
                     /locus_tag="PLW25_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOS54267.1"
                     /translation="MAMIIVMIGAPWLGALVVWLVGDRREKLQHWLAVVFSIVAAAAS
                     LVLIPKVTSETAVKIPMGGAFGEFTFVPDGLGVFLTAIATVIGCLAVVFSTSYMKGEA
                     SLGRYYALVLFFIGAMVGLVLTSNLLLIFFFWEITAFCSYALIAFHNDDPKAVRGGVK
                     ALIITSTGGVGLLAGALVLFAYTGSTDINAFIGAAGKIPANILSLLAFGFLVAAAAKS
                     AQFPFQTWLPDAMEAPSPISALIHAATMVNAGVYLLARTYPVFQNVPYWREAVMVVGT
                     LSAILAAVMALTANDLKRVLAYSTVSQLGYMVYAIGAGGVFASQFHLFSHSIFKALLF
                     LGAGAVIHSIGTRDMTEMGGVGKKLPLVKWVFLIGSLALAGLPVLNGFWSKELVLDAG
                     LKGGPTWLYVLMAIVAGLTALYTARCVGLVFYGESRSEYHVHPVGLGMKAALIPLATA
                     ALVSWIVVGPFSEMLGAHSLPFHEIKKMPLAELFHEILTLPTLVTLIVIGLGILLWFT
                     RSKLEGLTNGLRSIRWASEKS"
     gene            complement(1599..1910)
                     /locus_tag="PLW25_06985"
     CDS             complement(1599..1910)
                     /locus_tag="PLW25_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HOS54268.1"
                     /translation="MNLSLPLIILLTIFCLAGIGLYCLLITRNLIKVVVALQLIVKGV
                     VLAFILAGNLSGQMNTAQTLALTVIVADTIIAVVGLAFAVQIRLRSGSLDIKSLASLK
                     R"
     gene            complement(1907..>2383)
                     /locus_tag="PLW25_06990"
     CDS             complement(1907..>2383)
                     /locus_tag="PLW25_06990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOS54269.1"
                     /translation="LVGMGILACAILSVLSRRLLISAIWLALTSALVAVMIYLLGAPH
                     IAVIELSVGAGLVTVLFVFAINIAGEDTSVLKSLIPMPVAVGAVALAVGLGLVLILRA
                     NPGAVLTSPAPERSVILWQSRYVDLLLQVVLIFAGVLGVIGLLADKVKPHYEEETK"
     assembly_gap    2384..2483
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2509..3426
                     /locus_tag="PLW25_06995"
     CDS             2509..3426
                     /locus_tag="PLW25_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54270.1"
                     /translation="MKRAVSISLGSSSRNKSVDINLLGETIRMERIGTDGDMQKATDL
                     YTELDGKVDAFGVGGALLGIMVGNKWTPMYSVQSLIKNVRQTPVVDGTGLKMTLEKKV
                     ASVINNSLPGYVKEKKAFVAVALDRWGSAEAFMNDGYECVFGDLMFALGLGLPVRKRS
                     TLINLANILLPVLNRLPFSLLYPTGEKQNQRQPRFSEFFEWATVVAGDCHYLWRHMPE
                     RLPDRVIVTNTTTPADQDFFRSAGIKYLITTTPIIDGRSFGTNMIEAAILAALGRKDP
                     VDYARPGNYFNLMEEMLEKIPLMPQVKEL"
     gene            3427..4191
                     /locus_tag="PLW25_07000"
     CDS             3427..4191
                     /locus_tag="PLW25_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOS54271.1"
                     /translation="MDALVLAGGGVSKSDPLYPLAGDQPKSMVPIAGKPMVQWVLDAL
                     SSAELVDSVAVIGLDDSCGLHCVKRLTYLPDTGSLLQNIQRGAEHFSQVTPADSHILS
                     VSADVPAVTPEIIDACIRIFNAREFDIYYSVIERSAMEKRFPDSRRTYLKLKDGEVCG
                     GDVNCIRKKAALDPDSAWSGLIERRKNPIKQASMIGWGTLLLLMTGMLTLDNAASRVC
                     QRLGIQGKALKMPYPEIGMDVDKPFQHNIVERDLLR"
     gene            4201..4746
                     /locus_tag="PLW25_07005"
     CDS             4201..4746
                     /locus_tag="PLW25_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOS54272.1"
                     /translation="MRFERLLQQDIQITERLRLTNPDSRLKPIAIFLAHSGDSWFILL
                     GLLIIWFLSVGLWRSMTALMAAGAVGLALVVLAIKFTIRRRRPEGKWGAIYRNTDPHS
                     FPSGHAARTAMLAVIGLVIGPLWFGLLMLVWAPLVSLARVWMGVHYFSDIVAGILLGI
                     LAGVLVLQLAPWLSATFPYIF"
     gene            4813..4906
                     /locus_tag="PLW25_07010"
     tRNA            4813..4906
                     /locus_tag="PLW25_07010"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4847..4849,aa:Sec,seq:tca)
     gene            4937..6136
                     /locus_tag="PLW25_07015"
     CDS             4937..6136
                     /locus_tag="PLW25_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOS54273.1"
                     /translation="MESSDDSEILEHIVRRVFQIEDITYGTNQQAYRVRYRGHLLQPD
                     SATAYDQLSALLKPYQLTPLFRVEEKKQVIYLVSALPAPKPGRPLVNLLMFLLTLVSV
                     LVSGGLTSFEGELSKQPFTAALELFRAGWPFAVSLLAILAAHEFGHYFAGRAHGVHVT
                     LPFFIPLPFTPLGTMGAFINMKEIPRNRRVLMDIGVAGPIAGYIVSILVILIGLSLSR
                     LDTLPLHFPPGHGVQMEGNSLTYLLLKYIHFGQVLPQPASYGNTAPLLYWLRYFFTGR
                     PFPLGGLDVMIHPVAWAGWAGLLVTSLNLIPAGQLDGGHIFQLLFGAKWSRRILPFIL
                     VGLVGLGFVWSGWWLWAMLVFLFGRVYAEPLDQITQLDPLRKALGFTALVIFLLTFIP
                     VPLSVIA"
     gene            complement(6150..>7492)
                     /locus_tag="PLW25_07020"
     CDS             complement(6150..>7492)
                     /locus_tag="PLW25_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54274.1"
                     /translation="GLQIDPEKIKARVGSAVDVVDDVTEKVKRKSSSFYEKLFPSEVD
                     TPRSLLLLLAIVIPLIVVSAAGLVYIRRGRTQQFNYYYQQGQQYFIQSEAQKEDEIMY
                     LFNLQAAMMYMEKASEFGQTADSIALSSTIQSQLDAIQGVARLEITSLNMNDVLSKVN
                     ITQMVATTNDLYLLDGQSGRALRYSLSGNTYIKDDLFDCGPNPDNPLNRIGALVDILP
                     LPAGNSFGATLFGIDAYGNIEFCIPGKSGTISALIAPEVGWQGIKAIAMYQNYLYVLD
                     PGNNGVYMYPGAGILFEEKPTLFFDHSIPDLKNTVDMEVNADELYLLRANGEMVECTY
                     SHMKDYKLTECEDPAPYSDMRSGNEPQAITFPDAQFVQMRLTAAPDSSIYLLDTHGSS
                     LFHFSLQRNLQKIIQPLFVDSGYRPKFAASSMAISPSKMVFLAFGNQIFYGPLP"
BASE COUNT         1717 a         2107 c         1921 g         1647 t
ORIGIN      
        1 aaactctttt ctgaagccca gcggatgctg cgtaaaccgt tggtcaatcc ctccagtttt
       61 gaccgtgtga accacaacag tatgcccaac ccaattacga tcagggtaac cagcgtgggc
      121 agggtcaaaa tttcatgaaa gagctcagcc aagggcattt ttttaatctc atggaaagga
      181 agtgaatgag cccccaacat ttcagaaaat gggcctacca caatccagct caccaacgcg
      241 gctgtcgcca gcgggataag cgccgccttc atgcccagcc ctaccgggtg aacgtgatac
      301 tcgctgcgcg actcgccata gaacaccagc ccaacgcaac gcgcagtata cagagcggtg
      361 aggccagcca caatcgccat caaaacatac aaccaggtgg gtccgccttt caggcccgca
      421 tccagaacca gctctttgct ccaaaatcca ttcaacactg gcagccccgc cagcgccagt
      481 gaaccgatga ggaacaccca tttcaccagc ggcagttttt ttcctacccc tcccatctct
      541 gtcatatcac gagtgccaat ggagtggata accgccccag ctccaaggaa gagcaaggct
      601 ttgaaaatgg agtggctgaa tagatgaaac tgactggcga aaacaccgcc ggcgccaatg
      661 gcgtacacca tatacccaag ctggctgacg gtcgaatacg ccaatactct tttcaggtca
      721 ttggcggtca acgccatcac tgctgccagg atagcagaaa gcgtgcccac aaccatcacc
      781 gcttctcgcc aatagggcac attctgaaaa acagggtaag tgcgcgccag caggtacacg
      841 cccgcgttaa ccatcgtcgc ggcgtgaatg agggcgctga tcggggaagg cgcttccatc
      901 gcatccggca gccaggtctg aaagggaaac tgagcggatt tcgccgccgc ggccaccagg
      961 aagccaaacg ccagcagaga gaggatattg gcgggaattt tgccagccgc gccaatgaaa
     1021 gcgttgatgt cggtactccc ggtgtaagca aagagcacca gcgcgcctgc cagcaacccc
     1081 acaccgcctg ttgaggtgat gatcaacgcc ttgacgccgc cgcgtaccgc tttcgggtca
     1141 tcattgtgaa aggcgatcaa cgcataagag caaaacgcgg tgatttccca gaagaaaaag
     1201 atgagcagca ggttgctggt aagcaccagc cccaccatcg cgccgatgaa gaaaagcacc
     1261 aaagcgtaat aacgtccaag cgaggcttca cctttcatat aactggtcga aaagaccacc
     1321 gccaggcagc caatcaccgt ggcgatcgcg gttaaaaaga cacccaaccc atcaggcaca
     1381 aaggtaaact cgccaaaagc cccacccatc ggtatcttca ccgcggtttc actggtgacc
     1441 tttgggatca acacaaggga ggctgcagca gcgacgatag agaagacaac cgccagccaa
     1501 tgttgcagct tttcacggcg gtcgcccacc agccaaacca caagagcacc gagccagggt
     1561 gcgccaatca ttactatgat cattgccatt gtcatgggtt acctcttcag gctcgcaagg
     1621 gatttaatat ccaggctgcc gctgcgcagg cggatctgca ccgcgaacgc cagccccacc
     1681 accgcaatga tcgtatccgc cacgatcaca gtgagcgcga gggtctgggc tgtgttcatt
     1741 tgtccgctca agttaccagc gaggatgaac gccagcacca ctcctttcac gatcaactgg
     1801 agagcgacaa ccactttgat caggttacgg gtgatcagca ggcagtacag gccaataccc
     1861 gccaggcaga agatagtaag cagaataatc agagggagag acaggttcat tttgtctcct
     1921 cctcataatg gggtttgacc ttatcagcga gcaacccgat cacccctaat accccggcga
     1981 agatgagcac aacctggagc aacaggtcaa cataacgtga ctgccataag ataacggatc
     2041 tctcaggagc aggggaggtg agaaccgcgc cagggttcgc gcgcaggatg agcaccagcc
     2101 ccagccccac cgccagcgcc acagcgccaa ccgccacggg catggggatc agcgacttca
     2161 gcactgaggt gtcttcgcct gcgatattga ttgcaaagac aaagaggacg gtgaccagcc
     2221 ccgctccgac gctcaattca atcaccgcga tatgcggcgc tcctaacagg tagatcatca
     2281 ccgccaccag cgcgctggtc aatgccagcc agatggcgga aattaacaag cggcgggaaa
     2341 ggacggaaag gatggcacag gccaggatgc ccattcccac taannnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnatcttat caaacatcga ggtagcaaat gaaacgagcc
     2521 gtcagcatca gccttggttc ttccagccgt aataaaagcg tcgacatcaa cctgctcgga
     2581 gagacgattc gcatggagcg cattggcacc gatggcgata tgcaaaaagc caccgacctt
     2641 tacactgagt tggacggcaa agttgatgct ttcggcgtcg gtggagcgct gctgggcatc
     2701 atggtgggaa acaaatggac ccccatgtat tccgttcaat cgctcattaa aaatgttcgc
     2761 cagaccccgg tggtggatgg cacaggcctt aaaatgacgc ttgagaagaa agttgcctcg
     2821 gtaattaaca acagcctgcc gggatatgta aaagagaaaa aagccttcgt tgctgtggcg
     2881 ctcgaccgct gggggagcgc agaagccttt atgaatgacg ggtacgagtg tgtctttggc
     2941 gatctgatgt tcgccctcgg tttggggttg cccgtgcgca aacgctctac gctcatcaac
     3001 ttggcgaata tattgctccc agtgctcaat cgactgccat tctccctgtt ataccccacc
     3061 ggagaaaaac aaaatcaacg ccagccgcgt ttcagcgagt tcttcgagtg ggcaacggta
     3121 gtcgccggag attgccatta tctttggcgc cacatgcctg aacgcctgcc tgacagggtc
     3181 attgtcacca acaccactac tcccgccgac caggatttct tccgctcagc tggcattaaa
     3241 tacctcatca ccaccacacc cattatcgat ggacgctctt ttggtaccaa catgattgaa
     3301 gccgccatcc ttgccgcgct cggcagaaaa gaccctgtgg attacgcccg accgggaaat
     3361 tacttcaacc tgatggaaga aatgcttgaa aaaattccct taatgcccca ggtcaaggag
     3421 ctttaaatgg acgccctcgt gctcgcgggc ggcggcgttt ccaaatccga ccccctctac
     3481 cctctcgctg gcgatcaacc caaatcaatg gtccccattg ccggtaaacc aatggtccaa
     3541 tgggtgttgg atgccctttc aagcgctgaa ctggttgatt ctgtggccgt cattggtctg
     3601 gatgactcct gtggtttgca ttgcgttaaa aggctcacct acctgccgga tactggctca
     3661 ttattgcaaa acatccagcg cggcgctgag cacttttcac aggttacccc ggcagacagt
     3721 cacatcctct cagtgtctgc ggacgtgccc gcggttaccc cggaaatcat cgacgcctgc
     3781 atacgcattt ttaacgcgcg ggagtttgat atttattact ccgttattga aagaagtgcc
     3841 atggaaaaac gttttccaga ttcacgccgc acctacctca aacttaaaga cggtgaggtc
     3901 tgcggcggag acgtgaactg catcaggaaa aaggccgcgc tcgaccccga cagtgcctgg
     3961 tctgggctga tcgaacgccg taagaacccc atcaaacagg cgtcgatgat tggctgggga
     4021 accctgttgc tattgatgac gggtatgttg acgctggaca acgcagcctc ccgcgtttgt
     4081 cagcggttgg gcattcaggg gaaagcactg aaaatgccct accctgagat cggcatggat
     4141 gtggataagc ctttccagca caacatcgtt gagcgtgacc tgttgcggta gaattgcccc
     4201 atgagatttg aacgactgct gcagcaggac atccagataa ccgaacgcct gcgcctgacc
     4261 aaccctgatt cccgtctgaa acctatcgcg atttttcttg cgcactcagg cgactcctgg
     4321 ttcattttat tgggattgtt gatcatttgg ttcctgtctg tcggtctgtg gcgctcaatg
     4381 acggcgctga tggccgccgg tgcggtgggc ctggcgctgg tggtgctcgc catcaagttc
     4441 accattcgca ggcgccgccc cgagggaaag tggggggcca tttaccgcaa taccgaccca
     4501 cactcctttc cttccgggca tgccgccaga accgccatgc tggcagtgat cggcctggtt
     4561 atcggtccct tatggttcgg tttactcatg ctggtgtggg cgccattggt gagcctggcg
     4621 cgggtttgga tgggcgtgca ttatttttca gatatcgtcg cgggcattct gctcggcata
     4681 cttgcaggcg tgctggttct gcaattggcg ccctggttga gcgcgacctt cccgtatatt
     4741 ttttaatctc cagccaggaa gatagcggta ctcaccttgc caaatcaaat tggctgcggt
     4801 attcttaaca gcgggagtgg atgagccccg gtgggttccc cggtcttcaa aatcggtggc
     4861 gggtcgttat gcgggccgcg gtgggttcga ctcccatcca ttcccgcctg attatttaaa
     4921 gatacaatgg agcaaaatgg agtcatcaga cgatagcgaa attctggagc acatcgtgcg
     4981 ccgggttttc cagattgaag atatcaccta tggtaccaat caacaggcct accgcgtgcg
     5041 ttaccgcgga catttgttgc agcccgattc agcgactgct tatgaccagc tttctgcttt
     5101 gctcaaaccc taccagttaa cccctctttt cagggttgaa gagaaaaaac aggtgatcta
     5161 cctggtgagc gccctgcctg cacctaaacc tggccgcccg ctggtgaacc tgctgatgtt
     5221 cctgctcacc ctggtgagcg tactcgtttc aggcgggtta accagttttg aaggtgaact
     5281 ctcgaagcag cctttcacag ccgcgctgga acttttccgc gccggctggc cgtttgccgt
     5341 cagcctgctg gccattctcg ccgcccatga atttggtcat tactttgccg ggcgcgccca
     5401 tggcgttcac gtaaccctgc cgtttttcat ccccctgccg ttcaccccgt tggggaccat
     5461 gggagctttt atcaacatga aggaaatccc gcgcaaccgc cgtgttttaa tggacatagg
     5521 tgtcgccggg cccatcgccg gctacatcgt ttccattctg gtgatcctta tcggtctatc
     5581 gctctctcgc ctggatacgc tcccgcttca ttttccaccc gggcacggcg tacagatgga
     5641 aggcaattcg ctcacctatc ttttgctcaa atacatccat ttcggtcagg tactgcccca
     5701 accagccagt tatggtaaca cggcaccgtt gctctactgg ctgcgttact tcttcaccgg
     5761 gcgccccttc ccgctgggtg ggctggatgt gatgatccac ccggtcgcct gggcgggctg
     5821 ggccgggctg ctggtcacct ctctcaacct tattcctgcc ggtcaactcg atggaggtca
     5881 catcttccag ctgctgtttg gcgccaaatg gtcacgcagg attctgccgt ttattctggt
     5941 cgggttggtg ggtctgggat tcgtttggtc gggctggtgg ctgtgggcta tgctggtgtt
     6001 cctcttcggc agggtttacg cggaaccgct tgaccagatc acacagcttg acccgctgcg
     6061 taaagcgctg ggcttcaccg cgttggtgat ctttttactc accttcattc cggtgccgct
     6121 cagcgttatc gcgtaatttc agctcaactt cagggcaacg gtccgtagaa aatctggtta
     6181 ccaaacgcca gaaataccat cttgctcgga gagatagcca tactgctggc cgcgaatttc
     6241 ggacggtaac ccgaatccac aaaaaggggt tggatgatct tctgcaggtt gcgctgcagg
     6301 ctgaagtgga acaaactgct cccgtgtgta tccagtaagt agatggatga gtcgggcgcg
     6361 gcagtcaggc gcatctgcac gaactgcgcg tctggaaatg tgatggcctg tggttcgttt
     6421 ccgctgcgca tatcgctata cggtgccggg tcttcgcatt ctgtcagttt gtagtctttc
     6481 atatggctgt atgtgcactc gaccatctcg ccattcgcgc gcagaaggta aagctcatcg
     6541 gcgttgacct ccatgtccac cgtattcttc aggtcaggga tggaatggtc aaagaacaat
     6601 gtgggctttt cttcaaacag gataccggcg ccaggataca tatacacccc gttattcccg
     6661 gggtccaaaa cgtacagata attctgatac atcgctatcg ccttgatgcc ctgccatccc
     6721 acttctggcg caatgagcgc tgaaatcgtg cccgatttac cggggataca aaattcaata
     6781 ttgccatagg cgtcaatccc gaacagggtt gcgccaaaag aattcccggc ggggagcggt
     6841 agaatatcca ccaacgcccc aattctattt aacgggttat cggggtttgg tccacaatca
     6901 aaaaggtcgt ctttgatata ggtattaccg gaaagcgagt aacgcagcgc cctgccagac
     6961 tgaccatcca gcaagtacag gtcgttcgtg gttgccacca tttgcgtgat gttcaccttg
     7021 ctgagcacat cattcatgtt gagagaagta atttccagcc gtgccacccc ctggatggcg
     7081 tcaagctggg actggatggt ggaactcaag gcgatcgaat cagccgtctg gccaaattca
     7141 gacgccttct ccatatacat catcgccgcc tgcaggttga agagatacat tatctcgtcc
     7201 tctttttgcg cttcggactg aataaaatac tgctgtccct gctggtagta ataattgaat
     7261 tgctgggttc tgcccctgcg aatatagacc agccctgctg ccgagacaac gatcaatggg
     7321 atgacaatag cgagcaacaa caatagcgag cggggcgtgt caacttcaga agggaacaat
     7381 ttttcgtaga aagaagagct tttacgcttc actttctctg tcacatcatc cacaacatct
     7441 accgcgctgc ctacccgggc tttaattttc tcagggtcta tctgcagccc gg
//
LOCUS       DAOSPM010000074         7393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_10065_length_7393_cov_15.127283, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000074 DAOSPM010000000
VERSION     DAOSPM010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10065_length_7393_cov_15.127283"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLW25_07025"
     CDS             complement(<1..427)
                     /locus_tag="PLW25_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOS54275.1"
                     /translation="MKSHRLRSIIGFLMKQLTRTEFIGVENIPPEGGVLIACNHMSRL
                     DIPVLFITPNRPEITALVTTKYLKYPLLRWFIVTAEGIWLDRDTADFSAFRKAVEALK
                     QGKALGIAPEGTRSQTAKLIEGKPGTALLALRTGVPIVPV"
     gene            complement(481..>1678)
                     /locus_tag="PLW25_07030"
     CDS             complement(481..>1678)
                     /locus_tag="PLW25_07030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOS54276.1"
                     /translation="IFGLTTLVVVFICLVVLLLIAPLTREFSIEQIDQLHGILWITLA
                     AFLLTLPSLITSIRRLSGRPTRRVPRGMFLAASIALVCLAPLVLLGRLVSDSANWQWL
                     LKVINIFIAFIPLWWLLELGRLRLARCSAQRQWGVTAFSFYITLPVVVIVEIILFVLV
                     VLLAAVWLVQQPEFSPLLEQFERAFASGSFNPQNFTFDWLPLLEKPGVIMVIVLSISL
                     VVPVMEELLKPLGIWALVKRGLTPLDGFVAGMLCGASFALLESLFSLSSASGEDWLFT
                     ITGRAGTGLLHLTLTGFNGWALASSWKDGKTARVALTYILTVFVHGAWNLFAMVMGLS
                     MTGEELHLSVSPVLSASAPWVLGSLALGMLVMLMVMNWRLRRAPAVSPSPAAPPPLPA
                     ATRGLE"
     assembly_gap    1679..1778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1779..2314)
                     /locus_tag="PLW25_07035"
     CDS             complement(<1779..2314)
                     /locus_tag="PLW25_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOS54277.1"
                     /translation="MKKLAWLVWVSLFLVAACAPVSHDSAPIPASVTPSATLPFTPAL
                     FSTPIALSPQPAVTTQPTVAFDRSPAATCEVVSLVSAGVTGDFILPPEEQADWSYGSP
                     SPAVTVISYCQYQLPACRVLTNSLAELQQRYPDEVRVVLRQYPQPQTYDKSLLAAYAA
                     EAAGIENDFWEMNELLYS"
     gene            complement(2333..2956)
                     /gene="tmk"
                     /locus_tag="PLW25_07040"
     CDS             complement(2333..2956)
                     /gene="tmk"
                     /locus_tag="PLW25_07040"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOS54278.1"
                     /translation="MFITLEGPDGSGKSIQVPALAEFVRQLGYDVLTTREPGGTSIGD
                     QIREVIMSMKNKSMNPRTEILLFCAARAQIVSEVIRPQLEKGVVVISDRYADSTLAYQ
                     GYGHGLDLKVLRGILDFATGGLTPDLTLLLDVDVETGLSRRQQGGGEWNRLDDYALAF
                     HRRVRDGYLELAAAEPQRWVRIDASQAPQIVQQLMQEAVLRRLRPKV"
     gene            complement(2972..4603)
                     /locus_tag="PLW25_07045"
     CDS             complement(2972..4603)
                     /locus_tag="PLW25_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOS54279.1"
                     /translation="MKQIRITDDLNALMTVLPQHIIGALEKANNSDNLLEIILDLGRP
                     PVARFVDHELQLDEQDTKRDDIDHVVSRIGDFDADNRAGLERTLHRISAIRNRHGVIV
                     GLTCRVGRAVYGTIEIIQDLVESGKSILLLGKPGIGKTTMLRESARILAESKRVIIVD
                     TSNEIGGDGDVPHPAVGKARRMQVSTPSLQHEVMIEAVENHNPEVVIIDEIGRELEAM
                     AARTIAERGVQLVATAHGRTLENLLLNPTLSDLVGGIESVTLSDEEARRRGTQKTVLE
                     RRSPPTFDMLVELQERDRLAVHQDVAAAVDTLVRGYPLQPEIRWRDAANEVHVEKAPQ
                     PAAARGMAQGTRRTYSASGEEKSEPGSRHAESRYLRNASAPSLAYEQAVDLSERRPIN
                     KIRIYPYGVARNRLQQAAHRLGVPALIARDVSEADIVMTLRAYYRSRQQPITEAESRG
                     IPIYVLRANTINQMEQSLAEIFNLSEGNNQNLNEVTNQTESAIQAVLNGQRWVDLPPA
                     SSSIRRIQHEMARQAQLVSHSYGKDPNRRVRIFRE"
     gene            complement(4629..5531)
                     /locus_tag="PLW25_07050"
     CDS             complement(4629..5531)
                     /locus_tag="PLW25_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54280.1"
                     /translation="MISLHKAAYLIHGNPIGIAGVLARLNPTETIYTAVMEHPENGRK
                     YFCQVIHPPSSRSARFSYLAPDLIQNAEDYLPLIDFLCFQAGEMGALNVIAEIEEAHP
                     LFETLRRAGFCVFSWEAIWRLPDERSDPGSSAQWCAPAAADLNNVRNLYQTLVPPLVQ
                     NAEPFVNGNTPRLLFKQKDDTLAYVESISGSAGTYLVPLIHPSVEDIAPLLSGLIPHF
                     KENGKQLYVQVRSYQAWLSETLTELKAQPSPRFALMVKHLTVGQRNNLKPVQITRPDQ
                     RQADPAAPMLNNCTETGFFSEGLK"
     gene            complement(5588..6166)
                     /gene="pdxT"
                     /locus_tag="PLW25_07055"
     CDS             complement(5588..6166)
                     /gene="pdxT"
                     /locus_tag="PLW25_07055"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOS54281.1"
                     /translation="MKIGVLALQGGFAEHQAVLETLGVEVAQVRLPQHLAGLDGLIIP
                     GGESTTIGRLAVDFDLMQPLRLFGREHAIWGTCAGAIFLSKNAGREQPLLGLMDITIA
                     RNAFGRQAESFEADLDVPALLQVDPLDRPFHTVFIRAPLIESVGQGVEIFSQLEDGRI
                     VAARQGHLLATSFHPELTGDNRFHRYFLELAK"
     gene            complement(6174..7052)
                     /gene="pdxS"
                     /locus_tag="PLW25_07060"
     CDS             complement(6174..7052)
                     /gene="pdxS"
                     /locus_tag="PLW25_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOS54282.1"
                     /translation="MVGSFEVKKGLAQMLKGGVIMDVVNADHAKIAEDAGACAVMALE
                     RVPADIRADGGVARMSDPDMIQKIMESVSIPVMAKCRIGHFVEAQILEAIGVDYIDES
                     EVLTPADEAHHIYKHAFKVPFVCGCRNLGEALRRIGEGAAMIRTKGEAGTGDVVEAVR
                     HARSVLGEIRRLQQMPEEEVMAFAKEIGAPYELVTEVRTLGRLPVVNFAAGGIATPAD
                     AALMMQLGVDGVFVGSGIFKSGDPAKRAAAIVKATTHYNDPKILAEVSRNLGEAMVGR
                     QISAMPEEEKIATRGW"
BASE COUNT         1593 a         2048 c         2098 g         1554 t
ORIGIN      
        1 ccaccgggac gatgggaacc ccggtgcgca gagccagcag cgcggtaccg ggtttgcctt
       61 caatcagttt cgccgtctgg ctgcgggtgc cttcaggcgc gatccccaac gcttttcctt
      121 gcttcaaggc ctccacggcc ttgcggaagg ctgaaaaatc agccgtatca cggtccagcc
      181 agataccctc agcggtgacg ataaaccagc gcaataaagg gtattttaga tacttggtgg
      241 tcaccagggc ggttatctct ggccggttgg gcgtgataaa aaggacgggg atatccagcc
      301 gactcatgtg gttgcaggcg atcagcacac caccttcagg gggtatgttc tcgacgccga
      361 tgaattctgt tcgggttagt tgtttcatca aaaaaccaat aatgctgcgc aatcgatgtg
      421 acttcatctt attttgcacc ttcctgtggt taatagccgt atcctgacgg caggttcatt
      481 ttactccaat cccctggtgg cagccgggag cggtgggggc gcggcgggtg atggagaaac
      541 cgcaggcgcg cggcgtaagc gccagttcat caccattaac atcaccagca tccccagcgc
      601 cagagagccg agcacccagg gagcagaagc ggagagcacg gggctgacgc tcaaatgaag
      661 ttcctcgcct gtcatactta aacccataac catggcaaaa agattccatg cgccgtggac
      721 gaaaacagtg aggatgtagg taagggcaac gcgcgcggtt ttcccgtctt tccatgaaga
      781 agccagtgcc cagccgttaa aaccagtgag ggtgaggtgc agtaaacccg ttcctgcacg
      841 gcccgtaata gtgaaaagcc aatcttcccc gctcgcgctg ctgagagaaa acaggctctc
      901 aagcagggcg aacgaagccc cgcagagcat tccagcaacg aagccgtcta agggagtgag
      961 ccctctttta accaatgccc atattcccag tggtttgagc agttcttcca taactggcac
     1021 aaccagcgaa atactcaaca cgatgaccat gatgacaccg ggtttttcca gcaggggcag
     1081 ccagtcaaac gtgaagttct gcgggttaaa tgagccgctg gcaaacgccc gctcgaattg
     1141 ttccagcagg ggcgagaatt caggttgctg caccaaccac accgccgcca gcagcacaac
     1201 gagaacgaag aggataattt ccacaatgac cacaacgggc agagtgatgt aaaagctgaa
     1261 agcggtaacc ccccactgcc gctgggcgct gcatctcgcc agccgtagcc tccccagctc
     1321 caacaaccac cacaatggaa taaaggcaat aaaaatgttg ataaccttca acagccactg
     1381 ccagttggcg gagtcggaaa cgagcctgcc cagcaggacc aacggcgcca aacacacaag
     1441 cgcgatactg gcagccagga acatgccacg cgggacccgc ctggtaggcc ggccggaaag
     1501 acggcggatg gaagtgatga gcgatggcag ggtgagcagg aaagcggcca gcgtgatcca
     1561 cagtatgccg tgcaattggt cgatctgttc aatggagaat tcccgggtta acggggcgat
     1621 gagcagcaaa acgaccaggc agatgaagac caccacgagc gtggtgagcc cgaaaatgnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ggaatataac agttcattca
     1801 tctcccaaaa gtcattttca atcccagcgg cttcggcagc gtacgctgcc agcagtgact
     1861 tgtcgtatgt ctgtggctgg gggtactgcc gcaaaaccac gcgcacttcg tcaggatagc
     1921 gttgctgaag ttctgccagg ctgtttgtga gcacacggca cgccgggagt tgatattgac
     1981 aataggagat gacggtcacc gctggggagg ggcttccgta gctccaatca gcctgctctt
     2041 cagggggaag tatgaaatca cccgtaacgc ctgctgagac cagtgacaca acctcgcagg
     2101 tcgcggccgg gcttcggtca aacgccaccg ttggttgcgt ggtcaccgcg ggttgcggag
     2161 agagtgcaat gggtgtgctg aaaagcgcgg gcgtaaatgg taaggtggca gagggggtga
     2221 cagaagcggg gatcggcgcg ctatcgtgac tgaccggggc gcaagcggca accaggaata
     2281 gtgataccca taccagccaa gccagtttct tcaatctttc tcctggatgt ttctatactt
     2341 tcggtcgcag acgccgcaac acggcttctt gcatgagctg ctgcactatt tgtggcgcct
     2401 gactggcgtc gatgcgcacc cagcgctgcg gttcagcggc tgccagttcc aggtatccgt
     2461 cgcgtacgcg ccggtgaaac gccagcgcat aatcgtccaa ccggttccat tcgccgccgc
     2521 cctgttgccg cctggacaga ccggtttcca catccacatc cagcagcagg gtcaggtcgg
     2581 gagtcagccc ccctgtagcg aagtcgagga tgccgcgcaa caccttgagg tcgagaccgt
     2641 gaccgtagcc ctggtaagcc agggttgaat ccgcgtaacg gtcgctgatg accaccacgc
     2701 ctttttccaa ttgggggcgg atcacctcgg atacgatctg cgcccgggcg gcgcagaaca
     2761 acaggatctc ggtgcgcggg ttcatcgact tgtttttcat actcatgatc acttcgcgta
     2821 tctggtcgcc gatagaagta ccgcctggtt cacgcgtggt gagcacgtcg tagcccagct
     2881 gtctgacgaa ttccgccaga gccgggacct gtatggattt accgctgccg tcaggacctt
     2941 ccagtgtgat gaacatgctg tcttctttcg cctattcccg gaagatgcgc actctgcggt
     3001 tggggtcttt cccgtaggag tgcgaaacaa gctgcgcctg gcgcgccatc tcatgttgga
     3061 tacggcggat ggatgaagag gcgggcggta aatcgaccca gcgctgaccg ttaagcaccg
     3121 cctgaatggc agattctgtc tggttggtta cctcgttcag gttctggtta ttcccttcgg
     3181 agaggttgaa gatttcggcc agcgattgtt ccatctggtt gatggtgttg gcccttaata
     3241 cataaatggg gataccgcgg gattcggctt cagtgatggg ctgctggcgg gagcggtaat
     3301 aggcgcgcaa agtcatcacg atatccgctt cgctgacatc gcgggcaatg agggcgggca
     3361 ccccaaggcg gtgcgcggcc tgctgcagac gattacgcgc caccccgtaa gggtagatcc
     3421 gtattttgtt tatcggtcgg cgctcagaaa gatcaacagc ctgctcgtaa gccagtgagg
     3481 gtgcggaggc gttacgcagg taccggcttt ctgcatggcg tgaacccggt tctgatttct
     3541 cttcgccgct ggcagaatag gtgcgccggg taccctgggc catgccgcgg gcagccgccg
     3601 gctgaggggc tttttctacg tgcacctcgt tagcggcatc gcgccagcga atctcaggtt
     3661 gcagtggata accgcgcacc aatgtatcta ccgcggcggc tacgtcctgg tgcactgcca
     3721 ggcggtcgcg ctcctgcaac tccaccagca tgtcaaacgt gggcggagag cggcgctcga
     3781 gcacggtctt ctgtgtgccg cgccggcggg cttcttcatc tgacagggta accgattcga
     3841 tcccgcctac caggtcagaa agggtggggt tgagcagcag gttttctaat gtgcggccgt
     3901 gggcggtggc cactaattga acaccgcgct cagcgatggt gcgagcggcc atggcttcaa
     3961 gttcgcgacc aatttcatcg atgatgacca cttcagggtt gtggttttca accgcttcga
     4021 tcatcacttc gtgttgcagt gaaggagtag aaacctgcat gcgccgcgcc ttgcccacag
     4081 ccgggtgcgg cacatcgccg tcaccgccaa tctcattgga ggtatcgacg atgatcacac
     4141 gcttgctctc ggcaaggatg cgggcggatt ctcgcagcat ggtggttttg ccgataccgg
     4201 gtttgcccag cagcaagatg cttttacccg attccacaag gtcctggatg atttctattg
     4261 tgccgtaaac tgcgcgacct acgcggcagg tgagaccaac gatcacaccg tgccggttgc
     4321 gtatggcgga tatacggtgt agcgtgcgtt ctaaccccgc gcggttatcc gcatcgaaat
     4381 caccaatacg gctgacgaca tggtcaatat catcgcgttt cgtatcctgt tcgtccagct
     4441 gcagctcgtg atcaacgaag cgcgcaacag gggggcgacc aagatcgagg atgatttcca
     4501 gcaggttatc gctgttgttg gctttttcca gcgcgccaat gatgtgctga ggaagcacgg
     4561 tcatcagggc gtttaggtca tcagtgatcc tgatttgttt cataaagaac cgttcgcctt
     4621 tgttttatct attttaaccc ttctgagaaa aaaccggttt cagtgcaatt attgagcatc
     4681 ggggctgcgg ggtcagcctg gcgctgatca gggcgggtga tttgcacggg cttgaggtta
     4741 ttgcgctgtc caacggtcag gtgcttcacc atcaaggcga atcgtggtga gggctgcgct
     4801 tttagctcgg tcagcgtttc tgaaagccag gcctggtacg agcgtacctg cacgtaaagt
     4861 tgctttccgt tctctttaaa gtgagggatg agaccgctca ataacggagc aatatcctcg
     4921 acggaagggt gaatcagggg aaccaggtaa gtgcctgcgc tgccgctgat actctcgaca
     4981 taagccaggg tatcgtcttt ttgcttgaac aacagccgcg gcgtattacc attcacaaac
     5041 ggctcagcgt tctgcaccag cggtggcacg agggtctggt aaagattgcg cacattgttg
     5101 aggtcagcgg cggcgggagc gcaccactgg gcggatgacc ctgggtcgct tcgttcgtcc
     5161 ggcaggcgcc agatggcttc ccagctgaac acgcaaaaac cggcgcggcg cagggtttca
     5221 aacagggggt gcgcctcttc aatttccgcg atcacgttaa gcgcccccat ttcaccagcc
     5281 tgaaagcaca aaaaatcgat cagcggcagg taatcttccg cgttttggat caggtcgggc
     5341 gccagataag aaaagcgcgc cgagcggctg ctgggagggt gaataacctg gcaaaaatat
     5401 tttcgcccgt tttcagggtg ttccattacc gcggtataaa tggtttccgt ggggttcaag
     5461 cgcgccaata caccagcgat gccaatcggg ttaccgtgaa tgagataggc ggccttgtgg
     5521 aggctgatca cctgacggcg ccggctgagt attgcgggaa ggtcgtacca ggcaatcggg
     5581 cggacgttta ttttgccagc tccaaaaagt aacggtgaaa gcggttgtcg ccggtcaatt
     5641 cagggtgaaa cgaggttgcc agtaaatgcc cctggcgtgc ggcgacaatg cgaccgtctt
     5701 ccagttgaga gaatatctcg accccctgac caactgattc gatcaacggg gcgcggatga
     5761 acaccgtgtg aaaaggacga tcaagcgggt ctacctgaag cagagcgggg acatccaggt
     5821 cagcctcgaa gctttccgcc tggcggccaa aagcattacg cgcaatggta atgtccatca
     5881 atcctaaaag gggttgttca cggccggcgt tctttgagag aaaaattgcc cctgcacagg
     5941 tgccccatat ggcgtgttca cgaccgaaca ggcgcagcgg ctgcatcaaa tcaaaatcca
     6001 ctgccaatct accgatagtg gtggattcgc caccagggat gatgagcccg tcgagaccgg
     6061 cgagatgttg gggcaaccgc acctgcgcca cttctacacc cagtgtctcg aggacggcct
     6121 ggtgctcggc aaaaccaccc tgtaacgcca ggacgccgat tttcatctac cgcttaccat
     6181 ccgcgggtgg cgatcttctc ttcttcgggc atggctgaga tttgccgccc taccatggct
     6241 tcgccaagat tgcggctgac ttctgccagt atcttggggt cgttgtaatg ggtggtggct
     6301 ttgacgatgg ccgcggcgcg cttggctggg tcgccagatt taaagatacc cgaacccaca
     6361 aaaacgccat caacacccag ttgcatcatc agcgctgcgt ccgctggggt ggcgataccg
     6421 cctgcagcga agttgaccac tggcaggcga ccaagtgtac gcacctcggt taccagctca
     6481 tagggcgcgc ctatctcttt ggcaaaggcc atcacttctt cctcgggcat ctgctgcagc
     6541 ctgcggatct ctcccagtac tgagcgagca tggcgcacgg cttcaaccac atcaccggtg
     6601 cccgcttctc ccttggtgcg gatcatcgcc gcgccttccc ctatgcggcg cagggcttca
     6661 cccaggttgc ggcagccgca aacgaaaggc actttgaacg cgtgtttgta aatatggtgc
     6721 gcctcgtcgg ccggggtgag aacttccgat tcatctatgt aatccacacc aatggcttcc
     6781 agtatttgcg cttcaacaaa gtgaccaatc cggcattttg ccatcacggg gatactgacc
     6841 gattccatga ttttttggat catatcgggg tcgctcatgc gcgccacgcc gccatccgcg
     6901 cggatgtcag cgggcacccg ttccagcgcc atcaccgcgc aagcgccggc atcttcggca
     6961 atcttcgcgt ggtcagcgtt gaccacatcc atgatcacgc cgcccttcaa catctgcgcc
     7021 agcccttttt tgacctcaaa ggaacctacc atctgttcca taagaatacg aacctccttt
     7081 atcccacaaa catgaacttt gtcttcatta agaccattaa ttagatttta ccactcccga
     7141 gtacaggtcg caaccaacgc cttgaattat tggaagaaaa aacccgcctg atttgccttt
     7201 gcctccacga taaaaaaatc cctgctcggc agggatttct tggaaacact cagctgatgc
     7261 gctcgaaacg gctgccgggg aagccgcaaa ccgggcattt ttcaggcgcg ccgcgttttt
     7321 cggtgtaccc gcaaaccggg cagataaagt aatcgtattc ctctcctggt tgcccaagcg
     7381 aagccagcgc ttc
//
LOCUS       DAOSPM010000075         7334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_10156_length_7334_cov_12.292897, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000075 DAOSPM010000000
VERSION     DAOSPM010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10156_length_7334_cov_12.292897"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLW25_07065"
     CDS             <1..332
                     /locus_tag="PLW25_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54283.1"
                     /translation="AYAWGFELSGVPGEAMVQGLGLLFLMWSVPYLFAVVDPQKHFFS
                     LVTAVIMQFIGFAGESLLLTGLEEGHPLIVSSVTRFIIFDGAGLVALLLALWLVVFDR
                     KASIKKA"
     gene            complement(366..1157)
                     /locus_tag="PLW25_07070"
     CDS             complement(366..1157)
                     /locus_tag="PLW25_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOS54284.1"
                     /translation="MRNGFLILLIFFAFFTPFRSTLLIIGIDRAPEEGSVLGRSDTMI
                     LTTLPPVLPKMSLISIPRDLWVSIPGYGENRINTAHYFAELYQPGTGMQAAAGVIEAN
                     FGIAVPYVIRLRFEGFRNIVDAMGGVTIHLSEPMSGLDAGTHTLDGAQALAFVRDRAG
                     SDDFWRQKRGQMFISAAIQNMLNPVKWPRIPAVITATLQAVDSTIPFYAWPRLLYGAA
                     FSAVFGFESATFDRNWVTPWVTDEGAQVLLPNWEFITPFVKEVFK"
     gene            complement(1337..1837)
                     /locus_tag="PLW25_07075"
     CDS             complement(1337..1837)
                     /locus_tag="PLW25_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOS54285.1"
                     /translation="MSEEPRKIAFICSKGNLDMVYPALVMGWAALGNGIDVTIFFTFW
                     GLDMINKARVDHLEIPPIANTSMKMNMMGVPGYIGIPQIMGIIPGMTAFASALMKKKM
                     KGLEVPTVREYLEMLVDAGANLYACKMSVDMFDLKKEDFVDGVKDIVTAGDFMDMTEG
                     AQIVFI"
     gene            complement(1866..2180)
                     /locus_tag="PLW25_07080"
     CDS             complement(1866..2180)
                     /locus_tag="PLW25_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOS54286.1"
                     /translation="MATKKIAGVDVQVNEEGFLTNPAEWNKEIAVELAKEEGIELTDA
                     HWKVIDFCRSAAAVNGAAPTLRSITNGSGVSTKDLFALFPKGPAKKVAKISGLGKPEG
                     CV"
     gene            complement(2240..3481)
                     /locus_tag="PLW25_07085"
     CDS             complement(2240..3481)
                     /locus_tag="PLW25_07085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOS54287.1"
                     /translation="MKTFLILGAGTGGTMMANKLVKRLDPKEWKIILVDKDETHYYQP
                     GFLFLPFEYYKPEEVYQPKKKFVPSGVEFILSDVELIEPEANKVTLAKDKKVIHYDQL
                     LIATGTDIHPEEVDGMLDGWRQNIFDYFTFDGAMALREFLKNFKGGRVVLNIKEMPIK
                     CPVAPLEFVYLADYFFTQKGMRNKVDLIFTTPLSGPFTKPVSNKLLGDVMTKKGIHTE
                     AEFDVMEVDAAKNIIRGYDGRELEYDLLVSIPTNMGSDVIKRSGMGDDLYFVPTNKDT
                     LRSEKYENIWVIGDAANIPASKAGSVVHYAAETLIENILDALEGRPLSHKYDGHSTCH
                     ILSGFNKSLLIDFSYDTEPLTGLYPFPVIGPFPLLKESWFNYIGKLSFKWIYWNIMLR
                     GIPFPLPPEYSIAGKKQVAVS"
     gene            complement(3501..3848)
                     /locus_tag="PLW25_07090"
     CDS             complement(3501..3848)
                     /locus_tag="PLW25_07090"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOS54288.1"
                     /translation="MSDNLREEVVRLHAQVCSGLADPNRILIIYNLAEGPRCVNEIAA
                     SLELSQPTVSRHLKVLRERGIVRAERDGQTIHYRLSDARIIHALDILRSILADQLKDQ
                     VLLARQVNEKITP"
     gene            complement(4005..5909)
                     /locus_tag="PLW25_07095"
     CDS             complement(4005..5909)
                     /locus_tag="PLW25_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOS54289.1"
                     /translation="MTTLQRKKTIIKRILWILLGMLLLALLFVGVLFFIHVLRVTSSP
                     SIDILSPVKGQTAPSLTPFTIQAMAKQRRVPIRRLDLYLDGFLYGSVSGESDSLLGRW
                     NWTPVLTGDHELAFIATNTRGVMNIVQQKIEVTPVADTDHDGIPDSIDVCPEEYGYSE
                     SHGCLHPADIDGDGLIGDDDACPEVFGFAIDGGCPPDARPDTDGDGILDRADLCPEES
                     GLAELAGCPVESWFVDRDGDAIPDFLDRCPDEAGTGEALGCPHVTEGDADEDGIADAE
                     DDCPDEPGGRSTRGCPLEVSEDSDGDGVADSVDRCPEEAGVGSGDGCPIDDWLVDTDL
                     DFIPDLFDLAPDLPGLFEWLGLPLAGDTDSDGVPDSEDRCPELAGSPADCGCPRFTLP
                     LADIARQNLFSPFAFAGGAEPGGVGAGSKEAAYPNDIDRDGVHDRWDDECVGEHGEPA
                     QHGCAPVDDDDRDGIPNHIDRCDTIPGLYWGEYTDTYMFGCPREPAGEVNLELSITAA
                     RTPTEMQAIYCFAHPFPKSPVGLVNRIPPPYRNYSVDFLERYGYYLRLRDFRLNLSIP
                     NVKETEVITLYIACWGQPKGISLPARFLGEIYREVPVQDWDSQVRYAGGVGDSTMMEV
                     FYRVCRNHCP"
     gene            complement(5920..7002)
                     /locus_tag="PLW25_07100"
     CDS             complement(5920..7002)
                     /locus_tag="PLW25_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54290.1"
                     /translation="MKICRFLIPLMGLLLLAGCGLHTGGIGPGHAIWVDGPVEKETYV
                     NTSVSITVHSNYELSEVFIQYNLLSSSAVPLATVPMHQVGSTIFEGVTVWVPTLPGEY
                     NLRAYSADSVVSGARYLKVLPQPTPSSHSTPTAKSLPMTADTAIPTSAPPTILPDIQF
                     SADAYTLTAGECTFLRWSTVFVESAYLNGERVELMHSRQVCPVASSTYTLRGESHVGS
                     IEKSLTIRVSPPEVPPPSPPDTQSPSITEITKSAESIFDNTSCGLASNTISASVTDVS
                     GVSEVVLWFRAKNSSSATPGEWRSLTMSNTGGTTYQAALGISQLASSLSFYADGIVEF
                     YIIAKDSAGNFTQYGVRTFTTKMCLI"
     gene            complement(7238..7311)
                     /locus_tag="PLW25_07105"
     tRNA            complement(7238..7311)
                     /locus_tag="PLW25_07105"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7275..7277),aa:Val,seq:cac)
BASE COUNT         1673 a         1862 c         2067 g         1732 t
ORIGIN      
        1 gggcttacgc ctggggcttt gagttgagcg gcgttccagg cgaagcgatg gttcaggggc
       61 tgggtttgct ttttttgatg tggagcgtcc cctatctctt cgcggtggtg gacccgcaga
      121 agcatttctt ctcacttgtc acggctgtaa tcatgcagtt cattgggttt gcaggtgagt
      181 ctctgctcct gaccggattg gaagagggac accctctgat cgtttccagc gtaacgcgat
      241 ttatcatttt cgatggcgct gggttggtag cgctgttatt ggctttgtgg ctggttgtct
      301 ttgacagaaa agcgtcaata aaaaaagcct gacgggcagg ctttttacag gttaaggttc
      361 agcgattatt taaacacttc tttgacaaag ggggtgatga actcccagtt gggcagtagc
      421 acctgcgcgc cttcgtcagt cacccagggg gtcacccagt tgcggtcaaa agtggctgac
      481 tcaaagccga aaacagctga aaacgcggcg ccgtaaagca ggcgtggcca ggcgtaaaag
      541 ggaatggtgc tgtcaaccgc ctggagcgtg gcggtgataa ctgccgggat gcgtggccat
      601 ttgaccgggt tgagcatatt ctggatggcg gcggagatga acatctgtcc gcgcttctgg
      661 cgccaaaaat cgtcgctgcc ggcgcggtcg cgcacgaaag ccaacgcctg ggcgccatcc
      721 agggtgtggg tgccggcatc caacccagac attggctcgg acaggtggat ggtcacccct
      781 cccatcgcgt cgacgatgtt cctgaagccc tcgaaacgca ggcggataac gtaagggaca
      841 gcgatgccaa aattcgcttc gattacaccg gcagccgctt gcatgccagt tccgggctgg
      901 taaagctcgg cgaaatagtg cgccgtgtta atgcggttct cgccgtaccc ggggatgctg
      961 acccacaggt cacgcgggat ggagatcagg ctcattttgg gcagaactgg gggcagggtg
     1021 gtgaggatca tggtgtcact gcgccccaga acgctgcctt cttctggcgc gcggtcgatg
     1081 ccgataatca gcagcgttga ccggaaaggc gtaaaaaagg caaaaaagat gagcaggata
     1141 agaaagccgt tgcgcacagg gtgcctgcga cgcctttggc gctgcggtac tcctgctcct
     1201 gcatgggggt gtgttggagc gtactggtaa tattgattca ttgtgtcatt ttaacctgtc
     1261 tgcctggatc tgtcaggcag gcaaaaaaag ccggagtgga gaccaccccg gcggaatgcg
     1321 cataacaaaa ggtggattag atgaagacga tttgagcgcc ttcagtcatg tccataaaat
     1381 cacccgcggt gacgatgtct ttgacgccat caacaaaatc ttccttcttc aagtcgaaca
     1441 tgtccaccga cattttacag gcgtagaggt tcgcgccggc gtccacgagc atttcgaggt
     1501 attcgcgcac ggtgggcact tccaatccct tcattttctt tttcatcagg gcactggcga
     1561 aagcggtcat accggggatg attcccataa tttgcgggat gccgatgtaa ccaggcacgc
     1621 ccatcatgtt catcttcata ctggtattgg cgataggcgg gatttccagg tggtcaacgc
     1681 gcgccttgtt gatcatatcc agaccccaga aggtaaagaa aatggtcaca tcgatgccgt
     1741 tgccgagcgc tgcccagccc atcaccaggg cgggatatac catatccagg ttgcctttgg
     1801 agcaaataaa tgcgatcttg cggggttctt ctgacatttt ttcctctctc ttttagatga
     1861 tgagattaga cacaaccttc aggtttgcct aaccctgaga tttttgccac tttcttggct
     1921 ggccctttgg ggaaaagcgc gaaaaggtct ttggtgctca caccagagcc attggtgatg
     1981 gagcgtaagg tgggagcagc accattgacc gctgctgcgc tgcggcagaa atcgatgacc
     2041 ttccagtgcg cgtcggtcaa ctcgatgcct tcttctttag cgagctcgac agcgatttct
     2101 ttgttccatt ctgccgggtt ggtcagaaag ccctcttcgt tcacctgcac gtcaacccct
     2161 gcgatcttct ttgttgccat gtttgttcct ccttagaaaa attcagttaa tttgtttcaa
     2221 gaatttcagg cgcgggggtt caagataccg cgacctgttt tttaccagcg atggaatatt
     2281 caggcggcag cgggaagggg ataccgcgca gcatgatatt ccagtagatc cacttgaatg
     2341 agagtttgcc gatgtagttg aaccagcttt ctttcagcaa ggggaaaggc ccaatgacag
     2401 ggaagggata caggcccgtg agcggttcag tatcataact aaaatcaatg agcaggctct
     2461 tgttgaaccc agaaaggata tgacaggtgg aatgaccgtc gtacttgtgg gagaggggcc
     2521 tgccttcgag ggcgtcgaga atattctcaa tgagggtttc ggcggcgtag tgcactactg
     2581 aacctgcttt tgaagccggg atattcgctg catcgccgat gacccaaata ttttcatact
     2641 tttctgagcg cagggtgtct ttatttgtgg gcacaaagta caggtcgtca cccataccgg
     2701 agcgtttgat cacatctgac cccatgttcg tggggatgga aaccaacagg tcatattcga
     2761 gctcgcgccc atcatagccg cggatgatat ttttcgccgc gtcgacttcc attacatcaa
     2821 actccgcttc ggtgtgaatg ccttttttgg tcatcacatc tcccagcagc ttattggaca
     2881 cgggtttggt aaatggccct gaaagcggcg tagtgaagat gagatcgacc ttattgcgca
     2941 tgcccttttg tgtaaagaaa taatccgcca ggtaaacgaa ttctaatggc gccaccgggc
     3001 atttgatggg catttccttg atgttgagca caacgcgccc gcccttgaaa ttcttaagga
     3061 actctctgag cgccatggcg ccatcgaagg tgaaataatc gaagatgttc tgcctccagc
     3121 catccagcat gccgtctact tcttcggggt gaatgtcggt gccggtggcg atgagcagct
     3181 gatcgtaatg gatgaccttt ttatctttgg ccagggtcac cttgttggct tcaggctcga
     3241 tcaattccac atctgaaagg ataaattcaa cgccggaagg gacgaacttc ttcttgggtt
     3301 ggtacacctc ttcgggtttg tagtattcaa agggtaaaaa cagaaaacca ggctggtaat
     3361 aatgagtctc gtccttgtcg accaggatga tcttccattc ttttggatca aggcgcttta
     3421 ccagcttgtt cgccatcatg gttccgccgg taccggcgcc gagaatcaga aatgtcttca
     3481 tgcattttct ccgtagtggt ttagggtgtg attttttcgt tgacctgccg tgctaagagt
     3541 acctggtctt tcaactgatc ggccaggatg gaacgtaaga tatccagagc gtggatgatg
     3601 cgcgcatcac tgagacggta atggatggtc tgtccatctc gttcggcacg cacgatgccg
     3661 cgttcgcgca acactttgag gtggcgtgaa acggtgggtt gggaaagctc gagtgaggcg
     3721 gcgatctcgt ttacgcaccg cggaccttcg gcgaggttgt aaataattaa aatgcggtta
     3781 gggtccgcca gtccactgca gacctgggcg tgcagcctta ccacttcctc gcgcagatta
     3841 tcgctcattg tttatccctg taaattacca atatatacat atacgtttat gtgaatatat
     3901 tatacgacac atataaaaat cgcatatgtg acatttatca ccatttgggc ggagaataag
     3961 aaactgccgc tgttcaaggc ggcagttgga gggttgcgac caggctatgg gcaatggttg
     4021 cggcataccc ggtaaaacac ttccatcatg gtgctatcgc caacgccgcc ggcataccgc
     4081 acctgagagt cccagtcttg gaccggaact tcgcggtaga tctcacccag gaacctggcg
     4141 ggaagcgaaa tccctttagg ttgcccccag catgcgatgt aaagcgtaat cacttctgtt
     4201 tctttcacat ttgggatgga caaattcagc cgaaaatctc tcaggcgcag gtaataacca
     4261 tacctttcaa ggaagtcgac tgaatagttt ctgtatgggg gtggaatgcg gtttaccagc
     4321 cctacaggcg attttggaaa gggatgggca aaacaataaa tagcctgcat ttctgtaggg
     4381 gtcctggctg ccgttatgga tagctcgaga ttaacctccc cggcaggctc tcttggacag
     4441 ccgaacatat atgtatctgt gtattctccc caatacagac ctggaatggt atcgcagcga
     4501 tcgatatggt tgggtatgcc atcacggtcg tcgtcatcaa ctggagcgca gccgtgctga
     4561 gcgggctcac catgttcgcc cacgcattca tcgtcccatc gatcatggac accatcacga
     4621 tcaatgtcat tcggataggc ggcttccttc gagccggcac caacacctcc gggttcagca
     4681 ccccctgcga aagcaaaagg agaaaaaaga ttctgacggg cgatgtctgc cagagggaga
     4741 gtgaagcgcg ggcaaccgca atccgcgggt gaccccgcta actcagggca gcggtcttcg
     4801 ctatcaggaa cgccatccga atccgtgtcg cctgccaggg gcaaacctaa ccactcaaaa
     4861 agaccgggca gatcaggcgc caggtcaaac aggtcaggta tgaaatccag gtcagtatcc
     4921 acgagccaat cgtctattgg gcatccatcg ccgctgccaa cccctgcttc ttctgggcag
     4981 cggtcgacgc tatctgccac gccatcgcca tcgctgtcct cgctcacctc taacgggcaa
     5041 cctctggtgc ttctgccacc gggctcatcc gggcaatcat cctcggcatc cgcgatacca
     5101 tcctcatcgg catcgccctc tgttacatgc gggcagccca acgcttctcc tgttccagct
     5161 tcatcaggac agcggtcaag gaagtcagga atggcatcac catcgcggtc tacaaaccac
     5221 gattccaccg ggcagccggc gagctcagcc aggccgctct cttctggaca gaggtcggcg
     5281 cgatcaagaa tgccgtcgcc gtccgtatca gggcgggcgt cgggcgggca acccccgtcg
     5341 atagcaaaac cgaagacttc cgggcaggcg tcatcgtctc ctatcaaacc gtctccatca
     5401 atgtccgcag ggtgcaggca gccgtgggac tcgctgtaac catattcctc ggggcaaaca
     5461 tcgattgagt ctggtatgcc atcgtgatcg gtatcagcga caggagtgac ctcaatcttc
     5521 tgctggacga tgttcatcac gccgcgggta ttggtggcga taaaagcgag ttcgtgatct
     5581 ccggttaata caggcgtcca attccatctc ccaagtaaag aatcgctttc accactgaca
     5641 ctgccgtaaa ggaagccatc caggtacaaa tccaggcgtc tgatgggtac acggcgttgt
     5701 tttgccatcg cttgaatggt gaaaggggta agggatggcg cagtttgtcc ttttacggga
     5761 gagaggatat caatagaggg gctggaggtg acgcgcagga catgaatgaa aaagaggact
     5821 cctacaaaca ggagggcgag caggagcatc cccaggagga tccagaggat tcgcttgata
     5881 attgttttct tgcgctgcag ggtggtcatt ttctctcctt catatcaggc acattttggt
     5941 tgtgaaagtc cgcacgccat attgggtgaa gttgcccgcg ctgtctttcg cgatgatgta
     6001 aaactcaaca atgccgtccg cgtagaaaga gaggctgctg gcgagctgtg agattcccag
     6061 cgctgcctgg taggtggtgc cgcccgtgtt gctcatggtc aggctgcgcc attctccggg
     6121 ggtggcggaa gagctgtttt tggcgcggaa ccacaacacc acttcactca caccagaaac
     6181 atctgtaaca gaggcgctga tggtattact ggctaaaccg caggaggtgt tatcgaagat
     6241 gctttccgct gatttggtga tctctgtgat tgacggactc tgcgtatctg gcggagatgg
     6301 aggcggcact tctggcggag acacgcggat ggtgaggctt ttttctatgc tgcccacgtg
     6361 ggattctccg cgcagggtgt atgtgctgga agcgaccggg catacctggc ggctgtgcat
     6421 cagttctacc cgctcgccgt tcagataggc gctttcaaca aaaaccgttg accagcgcag
     6481 aaatgtgcat tctccagcgg tcagcgtgta agcgtctgcg ctgaattgga tatctggcaa
     6541 aatggtgggc ggagcggaag ttgggatggc ggtatccgcc gtcatgggca gagacttcgc
     6601 cgtcggcgtg gaatgagaag aaggcgttgg ttgggggagc acttttaaat agcgcgcacc
     6661 cgacacgacg ctatctgctg aatacgccct caggttgtac tccccgggta aggtgggaac
     6721 ccaaacggtt acgccttcaa aaatcgttga tccgacctgg tgcatgggca ctgttgccaa
     6781 agggacggca ctggaagaga gcaggttgta ctggatgaaa acctcgctca actcatagtt
     6841 ggaatggacc gtgatggaaa cgctggtgtt cacgtaggtt tccttttcaa ctggtccatc
     6901 cacccagatt gcatggcctg gcccgatccc tccggtgtgc agaccacagc cagccagcaa
     6961 tagcagcccc atcaatggaa tgaggaatcg acagattttc atgtgtgaac ctccctgttg
     7021 cctacccgat caggctaagc agaaaaaagg gcattgagaa acagcgatgt gggggtgttg
     7081 aggtcaggat tgtgggattg atgaagtgcg agttaccatt tctattatac gagaaaaagt
     7141 ggagaattga acctgcgagg ttaacccggg ttgttgcgca acaacccggg gcgggtaaaa
     7201 aaagaggctg gcgaaaatgc cagcctcagg ggtgaggtgg gcgcggaggg actcgaaccc
     7261 acgacctcac ggatgtgaac cgtgcgctct aaccaactga gccacgcgcc cctggagtac
     7321 ataaaaaccc tgaa
//
LOCUS       DAOSPM010000076         6498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_11963_length_6498_cov_16.115474, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000076 DAOSPM010000000
VERSION     DAOSPM010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11963_length_6498_cov_16.115474"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(911..983)
                     /locus_tag="PLW25_07110"
     tRNA            complement(911..983)
                     /locus_tag="PLW25_07110"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(948..950),aa:Asn,seq:gtt)
     gene            complement(1036..1362)
                     /locus_tag="PLW25_07115"
     CDS             complement(1036..1362)
                     /locus_tag="PLW25_07115"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOS54291.1"
                     /translation="MAETDLLITVLGMALVTYLPRLLPAWLLRGRALPPFITAWLRYI
                     PAAVLAALLLPSLLVEGGRLNLTWDNLYLWAALPAALAAWKWKSLFGTVLVGMALVAL
                     ARLLGW"
     gene            complement(1341..2195)
                     /locus_tag="PLW25_07120"
     CDS             complement(1341..2195)
                     /locus_tag="PLW25_07120"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOS54292.1"
                     /translation="MPGDIIAGVVPPTPKRCSTKSQTVQGCQYGSPAVLEGIIRRNSP
                     GSTMPQDKTLLPSLSKPLARLAPALPVVLGYLPIGFAFGVLAAAAGMGAFNSLLMSLL
                     VFAGSAQLVAVELVAQGLTPWSIVATTFIINLRHLLMSASLSPYMQEWRPAQVAGFTF
                     ELTDETFGVHSLRFERGETRAILALLINLVCHLAWVAGTLLGMLAGGLIRDVRPLGLD
                     YALPAMFIALLVMQIRGRVYLVVALMAAGLSLLVWSAGFTQWNVIIAAVLAAAAGAGA
                     ERWQKQTS"
     gene            complement(2152..2682)
                     /locus_tag="PLW25_07125"
     CDS             complement(2152..2682)
                     /locus_tag="PLW25_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54293.1"
                     /translation="MKKIWVFLVIGGVLALALGAASVVNAQAPVNTPQVEQGGGQGGF
                     GARAAGCGERGENEEVQKLMLNAWSDALGISVEELSNRKEAGENLAQIALSTGRTFEE
                     FRALRVSVHTSVAEQALKDGIIDQAQYQCHLKAVERQMHGRGNGNRPADGHGFTTRGR
                     GDRARGHHCGGRSANP"
     gene            3044..3703
                     /locus_tag="PLW25_07130"
     CDS             3044..3703
                     /locus_tag="PLW25_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOS54294.1"
                     /translation="MKKFIAVAGNIGVGKSTLVEMISERLECMPFYEPVAENPYLSDF
                     YKDMPAWSFHSQIFFLTHRLRIHQELVRTPGSVIQDRSIYEDAEVFAQNLFLQGHISE
                     RDYATYRSLYRTLAEYLPPPDLVIYLRASVPTLLKRIALRNRDYERSIEPAYLDHLNS
                     LYENWITHFTLCPVLTVPADELDYVAHTQHLELIISKVKEKLTGKEEVIFTREEANTP
                     D"
     gene            complement(3746..4012)
                     /locus_tag="PLW25_07135"
     CDS             complement(3746..4012)
                     /locus_tag="PLW25_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOS54295.1"
                     /translation="MADIIKVKANSRTAAVAGAIAGVMREHKHVDIQAIGAGAVNQAL
                     KALVLAKGYLAEEGIEIICSPEFVDVDIDGKVRTAIKIVVDPKN"
     gene            complement(4513..5505)
                     /locus_tag="PLW25_07140"
     CDS             complement(4513..5505)
                     /locus_tag="PLW25_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54296.1"
                     /translation="MRITRELLLKHARENSAALTAKDRSIICIYISGSLLQEDPFIGG
                     ITDIDLICVHNQPVARRREVLRLSHEVNLDLAHYEQEEFEPARQLRHNAWIGGWFENV
                     PIVLYDALRWYDFTRASATAQFWRPEYVASRARSFLNPARKTWNDLEEGNIPQGIKRV
                     TAYLGALRDTANAVAVFSGAPLAERRLLMELPARAAAAGLSGLFADFVSLFTSSEVSD
                     ENFTAWVSSYVGIFEALKQSTTAPVSLSPYRRGYYEKALNALYPHSPAAAVWLMVYTW
                     TKAAAALPRTEQPYKDWQELCRQLDLESRDLPTRLELFDAVLDTVEEAVDHLSA"
     gene            complement(5517..6152)
                     /locus_tag="PLW25_07145"
     CDS             complement(5517..6152)
                     /locus_tag="PLW25_07145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOS54297.1"
                     /translation="MYFAIEGVIGVGKTTLARLLQPQFEADLVLEVFEENPFLSNFYT
                     DRARYAFQTQIFFLLSRYHQQRRNIGERLGQAKNLISDYTFEKDSLFARINLTGDELD
                     MYFKVQEALAEKIIPADLVVYLRASTDTLMKRIALRDRPYERQMEREYIDLLNRVYDD
                     FYISSPQVSHVLTIETDELNIIERPDDLAFIRNRIREELRLAPYQPVLPIE"
     gene            complement(6305..>6498)
                     /locus_tag="PLW25_07150"
     CDS             complement(6305..>6498)
                     /locus_tag="PLW25_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54298.1"
                     /translation="AGLMCSARWWINWKNLPEVQPSKKAEKGSLPFLMDTSQPPDRCV
                     STGGVFYAEKAFFFYPFAN"
BASE COUNT         1571 a         1766 c         1689 g         1472 t
ORIGIN      
        1 ggtattttga cctttctacc tgcatggcgt tgttgatcag cactcgcatc agctccggga
       61 ttgcttcaag ccctttttct gctatttcgt ctgcaaaggt ataatcattt tggtgagtca
      121 ttttgttctc cttgtatggt ttagtcacct taaggatact tctgactcac cgcttttgtt
      181 aaggttcaac aatttacaga aaatatgtta catcaacaca tcaactagtt gtaatgagca
      241 aaattacttt ttttctataa tcatacaaat tacttattct gagtttcaag cattgcttga
      301 atatatacat tattattcac cgcgtgaaac ccttcaggtt tcacgcgata ctccagcccc
      361 aggcgcataa cgagacggtc aagagcgagg tgacgactct cttggctgtc gaggcgcccg
      421 gggtaataga agaagataaa ccgggaaacc ggaaatcccc cgtattacag tacgggggtg
      481 caaaatacag aaagataata aatgggtgga ttcataactt gacgatatga agaaaaacag
      541 aactgactct atgttctctt aatttcttga tctccgtttt cttggaagcg cagcaactat
      601 aaccataaga atcactaacg gaccaaataa ctcacctagc atttgatata cctgtttcat
      661 catgtctgga aaaaaaacta aaagcaaaat tccgagacca attaagaagg ctaaatttat
      721 tagtagtctc ttgagaaaac tcatttattt tctacctttg tactgttcag caaatctgta
      781 tgaaaaaatt cttgtgttaa ttttcatatc aatctccaat ctctacttat taacaaatta
      841 tagtatcaat gggtttcttg taataaaaaa gacaccctgg gcgattttgg gtgtctttct
      901 aaggctaagg ctcctcgtcg aggactcgaa cctcgaacct agcggttaac agccgcccgc
      961 tctgccgatt gagctaacga ggaaggcgac cattattata attcatgccc caaacctgtc
     1021 aagcgaagtt ttccctcacc atcccagcag gcgcgccagc gccaccagcg ccatgcccac
     1081 cagaactgtg ccgaacaggc tcttccattt ccacgctgcc agggcagcgg gcagcgccgc
     1141 ccacaggtac aggttatccc aggtcaggtt caggcgcccg ccctccacca acagagaagg
     1201 cagcagcagc gccgccagca ccgcggctgg aatgtaacgc agccaggcag tgatgaaagg
     1261 cggcagcgcg cgcccgcgca gcagccaggc gggcagcagg cgcggcagat aagtgaccag
     1321 cgccatgccc agaacggtga tcaggaggtc tgtttctgcc atcgttctgc tcctgctccg
     1381 gcggcggcgg ccagcaccgc cgcgatgatc acattccact gcgtgaaccc ggcgctccac
     1441 actaacaatg aaagccccgc cgccatcagc gccaccacca ggtagaccct gccgcgtatc
     1501 tgcatcacca ataaggcgat gaacatggcc ggcagggcgt aatccagccc cagcgggcgc
     1561 acatcgcgga tgagcccgcc agccagcatc cccaacagcg taccggctac ccaagccagg
     1621 tggcaaacca ggttgatgag cagcgccagt atcgcgcggg tttctccgcg ttcaaagcgc
     1681 agactgtgca cgccgaaggt ctcatcggtg agttcaaaag taaagccagc cacctgcgcc
     1741 gggcgccact cctgcatgta cggggagagc gacgccgaca tcaacaggtg acgcaggttg
     1801 ataatgaagg tggtggcaac aatcgaccag ggcgtgagcc cctgcgccac cagttcaacc
     1861 gccaccagct gcgccgagcc agcgaaaacg aggagagaca tcaacaggct gttgaaagcc
     1921 cccatgccgg ccgcggcggc cagcacgccg aaggcgaaac cgatgggcag gtatcccagc
     1981 accactggca gggcgggcgc gagcctggca agcggtttgg agagagaagg aagaagggtc
     2041 ttatcctgtg gcatggtaga accgggtgaa ttacgccgga ttataccctc taaaacagca
     2101 gggctgccgt attggcagcc ctgaacagtt tgcgattttg tggagcagcg tttaggggtt
     2161 ggcggaacga cccccgcaat gatgtcccct ggcacgatcc cctctgccgc gcgtggtgaa
     2221 cccgtgcccg tcggcagggc ggttgccgtt gccgcgaccg tgcatctggc gctcaaccgc
     2281 cttgagatgg cactggtact gcgcctggtc gatgataccg tccttgagtg cctgttcagc
     2341 cacagaagta tgcaccgaaa ccctgagcgc gcggaactct tcaaaagtac gcccggtgga
     2401 aagggcgatc tgcgccaggt tctcgccagc ctctttgcga ttactcaact cttccacgct
     2461 gatgccgagc gcgtcagacc aggcgttcaa catcaacttc tgcacttctt cgttttcacc
     2521 gcgttcgccg caaccagcag cacgcgcgcc aaaaccgcct tgtcctcctc cctgctccac
     2581 ctggggggtg ttcaccggtg cctgcgcgtt caccaccgag gcagctccta gcgccaaagc
     2641 cagcacgccg ccaatgacaa gaaataccca tatttttttc atttgcaact ccttttttgc
     2701 agagcttctc tctgcttgat taactcgatg atagccgcca gttatgaaga aaatgtgaat
     2761 ttatgatgag gattttgtga acgattgaat gccttctggg ttaaaaccaa tgagagcact
     2821 tgacctcgcg gttgctccga tccacgacct ccctcgattg tgagacaagg cgcaatgtta
     2881 taatcaaaac agggtatgaa ctcactggct gccgcagccc ggtagaaagc ctcttcaaag
     2941 agagctctgg ctgctgaagc gaaaaaatcc acctccaatc ttgacggttc gcaggattga
     3001 gggttctttc tctcaacaca cctttcacct tgctgaggat gttatgaaaa aattcattgc
     3061 ggtcgcgggc aacatcggtg taggcaagtc caccctggtg gaaatgatca gtgagcggct
     3121 ggaatgcatg cctttctacg agccggtagc agagaaccct tacctgtccg atttctataa
     3181 ggatatgccc gcgtggagct ttcactcgca gatatttttc ctcacccacc gcctgcgcat
     3241 tcaccaggaa ctggtacgca cgccgggttc agtaatacag gaccgcagca tctatgaaga
     3301 cgccgaggtt ttcgcccaga acctctttct gcaggggcac atcagcgaac gagattacgc
     3361 cacctaccgc tcgctttacc gcactctggc tgaatacctg cccccacctg acctggtgat
     3421 ctacctgcgc gcgtcggtgc ccacgctgct caagcgcatc gcgctacgca accgggatta
     3481 cgagcgcagc atcgaaccag cttatcttga ccatctcaac agcctgtacg agaactggat
     3541 cacgcacttc acgctctgcc ctgtgctcac tgtccctgcg gatgagctgg attatgtggc
     3601 tcatacccaa cacctggagt tgatcatcag caaggtgaaa gaaaaactga cgggcaaaga
     3661 agaagtgatc tttacccgtg aagaagctaa caccccggat tgaccagggc acgaaagata
     3721 aaattagtac tcccgcctgg cagacttagt tttttggatc gaccacaatc ttgatggctg
     3781 tgcgcacctt gccgtcaata tcgacatcca caaattcggg tgaacagatg atttcgatcc
     3841 cctcttctgc cagatacccc ttggccagca ccagcgcctt gagcgcctgg ttgaccgctc
     3901 cggcgccaat cgcctggata tccacgtgct tatgttcgcg cataacacct gctattgcgc
     3961 cggctactgc tgcagtgcga gaatttgctt tgaccttgat gatatctgcc ataagacctc
     4021 cctcccggat taaaccgggg aacaacgagc caaagccggt tggttttttt gtatttttat
     4081 tgtacacaat tcaactctca aatacaatca tcatcgcagc cttgtttgcg catcctgtta
     4141 acttatctct catggaagat taatctgtag tagtcgtcgt agtgggtgtg gatttgtgga
     4201 gaaacacagc cggaaggctt ttatccgcca accctgcatt gaggggtaac gttttcccct
     4261 gtatatgcta ttaggtggtc gaccgtcttt ccagccgtaa ctgtgcatga aatgtggata
     4321 aagccgtttt ttgggccaaa gagactgaaa aaagagacgc tgtggataaa gggcagaaaa
     4381 cagccttttt atccacaggg tttcgggcgg atttttcgaa aaaaagtgga aaaacgatgc
     4441 atttatccac agtttttttg aagttatcca caggttttag aggagttgtg tacaaacaga
     4501 acgggagaaa ggttaagcgg aaagatggtc aaccgcttct tccacggtat ccaacacagc
     4561 atcgaagagt tcaaggcggg tgggcaggtc gcggctttcc aggtcgagct gtcggcaaag
     4621 ctcctgccag tctttatatg gctgttcggt gcgtggcagc gcggcagccg ctttggtcca
     4681 ggtataaacc atcaaccaga cggcggctgc ggggctgtgc ggataaagcg cgttgagcgc
     4741 cttttcataa tacccccgcc gataggggga aaggctcact ggagcggtgg tggattgttt
     4801 gagggcttcg aaaataccca cataagaaga cacccatgcg gtgaagtttt catcgctcac
     4861 ttcgctgctg gtgaaaagac tcacaaaatc agcaaagagc ccgctgagcc cggcggcagc
     4921 ggccctggcg ggcagttcca tcagcaggcg gcgctctgcc aggggcgcgc cggagaaaac
     4981 agccactgcg ttggcggtat cgcgcagcgc tccaaggtag gcggtcaccc gtttgatgcc
     5041 ctggggaatg ttgccctcct cgaggtcatt ccaggtcttc ctggcggggt tcaagaaact
     5101 ccgcgcgcgt gaggcaacat attccgggcg ccagaattga gcggtggcgg aggcgcgggt
     5161 gaagtcatac caacgcagcg catcataaag cacgataggc acgttctcga accagccacc
     5221 gatccaggcg ttgtggcgga gttggcgcgc aggttcaaat tcttcctgct cataatgcgc
     5281 caggtcgagg ttaacttcgt ggctcaaccg cagcacttcg cggcgccggg caaccggctg
     5341 gttgtgaacg cagataagat caatgtcggt gataccgccg ataaaagggt cttcctgcag
     5401 caaagagccg ctgatgtaga tgcaaataat gctgcggtcc ttcgccgtga gcgcggcgct
     5461 gttctcgcgg gcgtgtttga gcaggagttc acgggtgatg cgcattcaac cctcttttac
     5521 tcgatgggta gaacgggctg gtaaggcgcc agccgcaatt cttccctgat acggttgcgg
     5581 atgaaagcca ggtcatcagg tctttcaatg atgttcagtt catcggtttc gatcgtaagg
     5641 acatgagaaa cctggggaga ggagatgtag aaatcatcat acacgcggtt caacaggtca
     5701 atgtattccc gttccatttg cctttcgtag gggcggtcgc gcagggcaat acgcttcatc
     5761 agcgtgtcgg tgctggcgcg caggtacacc accaggtcag ccgggatgat cttctccgcc
     5821 agggcttcct gcactttgaa gtacatgtcc aattcgtcac ccgtgaggtt aatgcgcgca
     5881 aagagcgaat ccttctcaaa ggtgtaatca ctgatgaggt ttttcgcctg ccccagccgc
     5941 tcgccgatat tgcggcgctg ctgatggtag cggctgagca gaaagaagat ttgggtttga
     6001 aaggcgtagc gcgcgcggtc ggtataaaaa ttgctcaaaa aagggttttc ttcgaagacc
     6061 tcgagcacca ggtctgcttc aaactgcggc tgcagcagtc tggcgagggt ggtttttcca
     6121 accccgatga ctccttcgat ggcaaaatac atacaccggc tccgtgaaca ctcccggctt
     6181 cgcgacggga agggatgttg ttgaattaaa aatataccat agaatatccc ggtgcagaca
     6241 gaataaccgc ggaaaagagc gccttgtgga gtttaccgtc ccataatcct ggcgcgggca
     6301 atttctaatt cgcgaagggg tagaaaaaga aggctttctc cgcgtaaaaa acaccccctg
     6361 tgctgacgca acgatcgggt ggctgtgaag tatccatcaa aaaaggcaga gagcccttct
     6421 ctgccttttt tgatggttga acctcaggca agtttttcca gtttatccac catctcgctg
     6481 agcacatcaa acccgccc
//
LOCUS       DAOSPM010000077         6479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_12013_length_6479_cov_14.311333, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000077 DAOSPM010000000
VERSION     DAOSPM010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12013_length_6479_cov_14.311333"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLW25_07155"
     CDS             <1..1131
                     /locus_tag="PLW25_07155"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOS54299.1"
                     /translation="TDVQLNDWSTESGKNDVHYGASFLYVTYLLDRFGEEITKEVVAD
                     QLDGFTSIDHVLAKNGLTDPDTGKIINADDFFVDWTIANLMNDTWFDDGRYAYRIYAS
                     APTASITETLTSCAGKQLDRTVKQYGTDYIELDCPGEEISLQFSGSPTVPVLPISSGE
                     NHFMWSNRADGSVTRLGREFDLTGVSAPITLRYETWYDLEVDYDYVYLLASIDGENWQ
                     IISTPSCSRKNVSGNNYGCGYNGYSGGWIEQEIDLSAYAGKKVTLSFEYVTDEAVTAE
                     GFLLDNIQLDALNYQADFESDDGGWLPEGFVNIENLIPQSFLVTILDTSGKSKIQKYA
                     LDAGDEISVTLPPLPSGQTYVVLVSGSARFTRQEASYRIDLR"
     gene            complement(1452..1667)
                     /locus_tag="PLW25_07160"
     CDS             complement(1452..1667)
                     /locus_tag="PLW25_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOS54300.1"
                     /translation="MKSIGKFLTGFFLGGIVGALAVLLFTPEPGDKMRQRIKDNCLHV
                     RDEVQNAARQRSEELKKELARLQKKTE"
     gene            complement(1719..2696)
                     /locus_tag="PLW25_07165"
     CDS             complement(1719..2696)
                     /locus_tag="PLW25_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOS54301.1"
                     /translation="MEALIDFEILVTLFWQNLGGWLEYPMQAFTFLGSELFFLLVMPA
                     LYWSIDPVIGFRAGMMLILSGGLNSILKMLFLTPRPYWVDSGVRAYSSETSFGMPSGH
                     SQNSAAIWGIIATTIRKKWAYVFAVLLVFFIGLSRIYLGVHFLHDVLTGWLAGVLVVL
                     LYLKLEAPVSSWLKKRALGTQLLISFIFAITLILLGFLSQQSAGRWVVPTTWTTLAEA
                     AGAEAPDPLNLDGVITIAGVAFGFTAGFAWWRKKFGAYEIKCSLVKKMARYAFGLIGI
                     VVLYFGLKLVFPEEPLLIGGIFRFIRYTLIGMWVTFFAPWFFRLFHLER"
     gene            complement(2733..>3414)
                     /locus_tag="PLW25_07170"
     CDS             complement(2733..>3414)
                     /locus_tag="PLW25_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOS54302.1"
                     /translation="LTFPQELLDYQYTPEDGSLDRLSHYSRLLLEKIPDEYMEAAAAH
                     ICPLDGDSQIKLSAMLEKGSIRNVTLQPHRSTMVPERIEDVVVLSKDATAIFVKEAEL
                     RNLFRIRAHDLWEMTAALCSYGCRAVVVKGQQGGYLLHDAYSSHKYLVPDYPVQASDP
                     TGENEVFCGAFLMAYQHTYDPLSAAITGSATASLKREASGPFSLSASLPGLAEARMET
                     IRNRVVQV"
     assembly_gap    3415..3514
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3515..3721)
                     /locus_tag="PLW25_07175"
     CDS             complement(3515..3721)
                     /locus_tag="PLW25_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54303.1"
                     /translation="MSRNPNWKSLTFLIGGSIGLLTGLAAAYLVVKKQEESGETRLLT
                     TGDGAKIGMGIVTLLKLISETGHR"
     gene            complement(3724..4176)
                     /locus_tag="PLW25_07180"
     CDS             complement(3724..4176)
                     /locus_tag="PLW25_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54304.1"
                     /translation="MENYQPIPSAIRPAAQIHRRQVFWQILLPILGTIFAVLAGLILV
                     IVSAGTPQSNAADWSIISTIFLILPWLFLGLIPLAFLFLCIWLLKKTRHFIAPYLVTA
                     GRYSRVLKIHNAKFTQAITAPFIQVRSVNAGITRLLRLALHIKSSPKE"
     gene            4625..5290
                     /locus_tag="PLW25_07185"
     CDS             4625..5290
                     /locus_tag="PLW25_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOS54305.1"
                     /translation="MMTEKTITAIRVQKRNPERVNIDLDGDFAFGLSRITAAWLKIGD
                     RLTQERIDALQREDAIEVAYQRALLLLGYRARSEQELRRKLTEKNFSTEQVDQVVRKL
                     KQTNLIHDGSFARAWVENRNNSHPRSQRLMRYELQRKGVAEEDIQRAIKTSAPDQELA
                     LRAAENYSRRLTGYDFKVFRNRLSGFLARRGFSYEVIAPVVSALWEQRSANNNTDEEK
                     EEE"
     gene            5293..>6479
                     /locus_tag="PLW25_07190"
     CDS             5293..>6479
                     /locus_tag="PLW25_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HOS54306.1"
                     /translation="MGKIETGLILLLVLGALLLAALLTILLVNIYKSKFKKEQSEKAD
                     AVILTAVEKAKTIELEARDKALKVLQESEQEANRRRQDILREDDRQQKRRAELDHRLE
                     RLELREQNLNKRQSALDKRANEIEKLYADQLEELQRIAQMTMDDAKQVLMTEAEKEAR
                     NDMARIIRSIEAEARTEGDKRAREIITDAIQRVASEHVVSVSTSLVTLPNEEMKGRIV
                     GRNGRNIRAFEQAAGVDVIVDDTPEAVTISCFDPVRREIARRSLGRLINDGRIHPAHI
                     EKILAEEEREVEKAIVEAGEEAAFDAGVAGMHPEVLKMLGRLKFRTSYGQNQLAHAVE
                     VARLSAVLAAELGADVEVARTGGLLHDLGKAMDHNTEGTHAQLGADFARRYGVNSRVV
                     NAMA"
BASE COUNT         1783 a         1460 c         1673 g         1463 t
ORIGIN      
        1 acagatgtgc aattgaacga ctggtcaaca gaatctggaa aaaacgatgt gcattacggc
       61 gccagttttc tttatgtcac ctacctgctg gaccgatttg gagaagagat taccaaggaa
      121 gttgtcgctg accagcttga tggatttaca agtattgatc atgttctggc gaaaaatggt
      181 ttgaccgacc cagacacggg gaagattatc aacgctgatg acttctttgt ggattggaca
      241 atcgcgaacc tgatgaatga cacctggttc gacgatggcc ggtacgccta tcgtatttac
      301 gcatccgccc ccaccgccag catcacagaa acgcttacct cgtgtgccgg taaacagctg
      361 gatcggacgg tgaaacaata tggaacagat tatattgaac ttgattgccc tggcgaagag
      421 atctcgctgc agttctctgg ttcaccaaca gtccctgtgc tgccaatctc cagcggagaa
      481 aaccacttca tgtggtccaa ccgcgcggat ggctcggtta ccaggctggg ccgggagttt
      541 gatctaaccg gggtcagcgc acctataacc ctgcgctatg aaacctggta cgacctggag
      601 gttgattatg actatgtgta cctgttggca tcaattgatg gagagaactg gcaaataatt
      661 tctacaccgt cctgcagccg gaagaacgtt tccggtaata attacggctg cggttacaac
      721 ggctactcag gcggctggat cgagcaagaa atagaccttt ctgcttacgc ggggaaaaag
      781 gttactttaa gtttcgagta tgtgacagat gaagcggtca ctgcggaagg atttttgctc
      841 gacaatattc aattggatgc gctcaactac caggctgatt ttgagtcaga cgatggcggc
      901 tggctgccag aggggtttgt gaatatcgaa aacctgatcc cgcagagctt tttagtaacc
      961 atactggata cgtctggtaa gtcaaagata cagaagtacg cccttgatgc cggggatgaa
     1021 atttcagtca ccctgcctcc tttgccttct ggtcaaacgt acgtggtact tgtcagcggg
     1081 tccgccagat tcacccggca agaggcatcc tatagaattg acttgcggtg aagtgttgcg
     1141 ttttcggata ttacctggtc ggaaaacagg tcggcttttt ttaagccgct gccatcttcc
     1201 tgaatgaggt tatggacgtc ttgccaggct tgagggcgtg ctttctcacc gccccagcga
     1261 gatggcggat aaacctagga gcaacctctg atggcttgat ggtcatattg cgtgatagaa
     1321 aaaagggcaa acttgcttta aaacacccgg cactgcataa cgttttttcc cgccagaaaa
     1381 aacctgagaa ataagagggc gtgaaaagaa aagcagttgc tggagttcaa gcaactgctt
     1441 ttggttgaag atcactcggt ttttttctgt aagcgcgcca gttccttttt taattcttca
     1501 ctgcgttggc gggcggcgtt ttgcacctcg tcacgcacat gtaaacagtt gtctttaatt
     1561 cgctggcgca ttttgtcccc gggttcaggg gtgaataaaa gcaccgccaa cgcacccacg
     1621 ataccgccca ggaaaaaacc agtcaagaac tttccgatgg atttcatagt tcctccaatt
     1681 tctcaattca cttaattata gccctttatg gcgtgtaatt aacgttcgag gtgaaacagt
     1741 cgaaaaaacc atggcgcgaa gaaggtaacc cacatcccga tgagcgtata cctgatgaaa
     1801 cgaaatattc cgccaatcag caagggttct tcaggaaaga cgagtttcaa accaaaatag
     1861 aggacaacaa tgcctatcaa accaaatgca taacgcgcca tttttttaac aagcgagcat
     1921 ttgatctcat atgcgccgaa tttcttacgc caccaagcaa acccagcggt aaaaccaaaa
     1981 gccaccccgg cgatggtgat caccccatca aggttgagtg ggtcgggggc ttcagcgccg
     2041 gctgcctctg ccaaagtggt ccaggtagtg ggtaccaccc acctgccagc tgattgctgt
     2101 gacaaaaatc ccagcaaaat taaggtaatt gcaaatatga atgaaatcag tagctgggtg
     2161 ccgagtgctc gttttttaag ccagctggat actggcgctt cgagcttgag atagagcagc
     2221 acgaccagca ccccggcaag ccaacccgtt aatacgtcat gcagaaagtg aacacccagg
     2281 taaatgcgtg aaagaccaat aaagaagacc agcagcaccg cgaagacgta agcccatttt
     2341 ttacggatag tcgtggcgat gataccccag atggctgccg aattttgaga atgcccagaa
     2401 ggcatcccaa atgaagtttc tgaggaatag gcgcgtacac cactgtcaac ccagtatggg
     2461 cgtggagtaa gaaagagcat cttgaggatg gagttcaaac ctccgctaag aattaacatc
     2521 atcccggcgc gaaaaccaat aaccgggtca atgctccagt aaagcgccgg catcaccagt
     2581 aaaaagaaca gttcgctgcc cagaaaggtg aacgcctgca tggggtattc caaccaaccg
     2641 cccaggtttt gccaaaacag tgtgacaaga atttcaaaat caattaacgc ttccattgtt
     2701 tcctcccaaa tgatgagcca atgaagtcaa gtttaaacct gtacaacccg attgcggatg
     2761 gtttccatac gggcttcagc caacccaggc aacgaagcag atagagaaaa cgggccgctg
     2821 gcttctcttt tgagtgaagc ggtggcgctg ccggtaattg ccgcgctgag ggggtcatag
     2881 gtgtgctggt aagccatcag gaacgcgccg cagaaaacct cgttttcgcc tgttgggtcg
     2941 ctcgcctgca ctggataatc tggaaccaaa tacttgtgtg aagaataagc atcatggagc
     3001 aggtatccgc cctgttgtcc tttaaccact accgcccgac agccataact gcacagagct
     3061 gctgtcatct cccacaggtc atgagcccgg atgcgaaaga ggttgcgcag ttccgcttct
     3121 ttcacgaaaa ttgcggttgc gtctttgctt aatacaacga catcttcaat tcgttccggc
     3181 accatggttg accgatgagg ttgcaaggtg acattccgaa tcgagccttt ctccaacatg
     3241 gcagacagtt taatctgact gtcgccatca agcgggcata tatgcgccgc ggcggcttcc
     3301 atatactcat ccggtatctt ttccagcaga agacgggagt aatgggagag cctatcgagc
     3361 gacccatctt caggagtgta ttggtaatcc aataattcct gaggaaaggt caggnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttatcg gtgaccggtt tctgaaatca
     3541 gcttgagaag tgtgacaata cccatgccga tcttggcgcc gtctccagtg gtgagcaagc
     3601 gggtttcacc gctctcctcc tgttttttta caaccaaata agcagctgcc agacctgtca
     3661 ataaaccaat tgaaccgccg ataaggaaag tcagagattt ccagttcgga ttacgagaca
     3721 taatcactcc tttggtgaag atttaatatg tagcgccagg cgaagcaaac gcgtgatccc
     3781 ggcattcact gatctgactt ggatgaacgg agcggtaatt gcctgtgtga atttcgcgtt
     3841 gtggattttt agcacccgac tataacgccc ggcagtaacc aaataggggg ctataaaatg
     3901 gcgtgttttt ttaagtaacc agatacataa gaaaagaaag gctaagggga tcagaccaag
     3961 gaacaaccag ggaaggatca agaaaatggt cgagatgatg gaccaatcag ctgcattgct
     4021 ctgaggtgtg cctgctgaca cgatgaccaa gatcaatcca gccagcacgg cgaaaatcgt
     4081 tcccaggatt ggtaaaagga tttgccagaa gacctgcctg cggtgaattt gcgctgcagg
     4141 gcgaatggcg gatgggatag gctgatagtt ttccatgcat ccattttagc attaatcacc
     4201 tgtctagttt tttaaagaag gaagaaaatg ttgagaaact caaaaataat gtataatact
     4261 cctcgtaaga cgtgattgag tggcttatta attgcgcgta gcaagctggc ttttcgataa
     4321 gaatactggg tcgaaaaata tttttacact ccggatggag ttggatgtag attaaataaa
     4381 ttgagattgg ataatgattc tcgaattcat gaactctaca agatttcaaa taacaccacg
     4441 gggacaggaa tgtattttgg aaaatcatca tcgggctatc tgttccaggg cgtcagggaa
     4501 atcgatccaa ttgaaacatc catgaagtaa aaatacacaa actgtgattg attgagtcac
     4561 agaaaaccag tttatttcga acagccagaa gaccattttg gaattttggc ttttttgttt
     4621 tacgatgatg actgagaaga cgataaccgc gatcagagtt caaaaacgca accccgaacg
     4681 agtcaatatt gacctggacg gcgactttgc ttttggcctt tcacggatta ctgcggcgtg
     4741 gttaaagatc ggagacaggc taacccaaga gcgcattgac gcattacagc gcgaagacgc
     4801 cattgaggtt gcctaccagc gcgcgctgtt gttgctcggg tatcgagcca ggagcgaaca
     4861 ggagctgcgc cggaaactga cagaaaaaaa cttcagcact gaacaggtgg accaggtggt
     4921 gcggaagttg aaacagacga acctgattca tgacggtagt tttgcccgcg cgtgggttga
     4981 gaaccgtaat aactcgcacc cgcgcagtca gcgcctgatg cgttatgaat tacagcgaaa
     5041 aggggttgct gaagaggata tacaacgcgc tatcaaaact tccgcgcctg atcaagaact
     5101 ggccctacgc gcagctgaaa actattctcg aaggctgacc gggtatgact tcaaagtctt
     5161 tcgaaatcga ttgagcgggt tcctggcaag gcgaggtttt tcttatgaag tcatcgcacc
     5221 ggtggtttcc gccctgtggg agcagcgaag tgctaacaat aatactgacg aagaaaaaga
     5281 ggaagaataa acatgggaaa aattgaaacg ggactgatct tactgcttgt tttaggagcg
     5341 ttgttgctcg cggcgctgct aacgattttg ctggtaaaca tttacaaatc aaaatttaaa
     5401 aaagaacaaa gtgaaaaagc agacgctgtg atacttacag cggttgagaa ggccaaaacg
     5461 attgaactgg aagcgcgaga taaagcgctg aaggtgctgc aagaatctga gcaggaagcg
     5521 aaccggcggc ggcaggatat tttaagagaa gatgaccggc agcagaaaag gcgcgcagag
     5581 ctggaccatc gccttgagag gcttgagcta cgcgagcaaa atttgaacaa gcgccaaagc
     5641 gcgctggata aaagagccaa tgaaatcgaa aagttgtacg ctgaccagtt ggaggaattg
     5701 caacgcatcg ctcaaatgac gatggatgat gccaagcaag tcttaatgac cgaagcagaa
     5761 aaagaagcgc gtaatgatat ggcgcgcatt attcgttcca tcgaggctga agcccgaacc
     5821 gaaggagaca aacgcgcccg cgagatcatc accgatgcca ttcaacgggt cgcttcagaa
     5881 cacgtggtga gcgtttctac ttcgctggtg acgctgccta acgaggaaat gaaaggccgc
     5941 attgttgggc gtaatgggcg aaatatccgg gcttttgaac aggcggctgg ggtggatgtc
     6001 atcgtggatg atacacccga agcagtgacc atcagttgtt ttgaccctgt gaggagagaa
     6061 atcgccaggc gttcactggg ccgcctgatc aatgatggca gaattcatcc cgctcatatt
     6121 gaaaagatac tggctgaaga ggaacgcgaa gtagagaaag ccatcgtaga ggcaggcgaa
     6181 gaagccgcct ttgatgccgg agtagcaggg atgcaccccg aagtgttgaa aatgctcggg
     6241 aggttgaagt tccgcacatc ttacgggcag aaccagctgg cgcacgcggt ggaggtggcc
     6301 aggctttcag cggtgctggc cgcggaatta ggcgcagatg ttgaggtagc ccgtacaggc
     6361 ggtttactgc atgacctggg taaagcgatg gatcacaata ccgaaggcac gcacgctcag
     6421 ttaggcgcgg attttgcccg gcgttacggg gtgaactcga gggtggtgaa cgcgatggc
//
LOCUS       DAOSPM010000078         5986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_13391_length_5986_cov_17.458102, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000078 DAOSPM010000000
VERSION     DAOSPM010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13391_length_5986_cov_17.458102"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2088
                     /gene="glgP"
                     /locus_tag="PLW25_07195"
     CDS             <1..2088
                     /gene="glgP"
                     /locus_tag="PLW25_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOS54307.1"
                     /translation="AHNMWWVWNHDGQRAFQFIDPRLWDLVAHNPVAFLRRVERHRLN
                     AAVGQSAYLEHYDRTLANFDAYIADRNTWFQRTYPQFTDKTIAYFSFEFGIHESLPVY
                     AGGLGILSGDHLKEASDLGLPLVGMGFVYDKGYFVQELTEDGWQEPRNYLIDYNMLPL
                     IALLDENRKPLTVTIDLPGRQVSARLWELEVGRNHLYLLDSNVETNAPADRQLTERLY
                     SNDLEVRISQEMLLGMGGVQALRLLGYDPGVWHMNEGHSTFLALERIREKMKTGTSFQ
                     AAWEEVKVTDVFTTHTPVPAGNDQFPLWLVDKYFGNYWPELGLTHDQFLDLGRQAQSW
                     GDTFVMPVLALKLSERANAVSELHGIVSRRMWHFLWPDREVEDVPIHYVTNGVHTRSW
                     LARRMGVLFDTYLGEDWREHLDEPDFWNNINRIPNEELWAVRRHLKNKLVNFTNERTR
                     QQWQNGQLTPSQAVAGGVFMEPYSLTIGFARRFATYKRANLVFKDFDRLMNIINDAHM
                     PVQIVFAGKAHPADEPGKLLIQQVYRAVKDTRSAGRLVFIEDYDINVARYLVQGVDIW
                     LNTPRRPNEASGTSGMKAALNGVLNFSILDGWWREGYNGKNGWAIGKEIEYTDSEAQD
                     AADAQSLYETLEKQIVPLYYERDENKIPQEWLKMVKECLRTLVPHFSLRRMLKEYTTD
                     YYLPAMKQEKAEW"
     gene            2101..2628
                     /locus_tag="PLW25_07200"
     CDS             2101..2628
                     /locus_tag="PLW25_07200"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOS54308.1"
                     /translation="MAYLSEPHAWLFALMIFALRVVDMSLDTIRVLFVVRGKKLIVWV
                     LGFFQSLVFVVAISSVLTQLNNILNVIGYATGFATGNLVGMLIEQRLAIGHILITIIS
                     SSRGSYIAEQLRSNGYAVTEIAGRGRDGTVFELHASVLRRDVPQVETIVLEADPQAFI
                     TAEDVRPVRRGFWRA"
     gene            complement(2701..3891)
                     /locus_tag="PLW25_07205"
     CDS             complement(2701..3891)
                     /locus_tag="PLW25_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HOS54309.1"
                     /translation="MMDNSDKNTAGEAQQLSENPADEAFSPAERLWNRILHLGLGETT
                     LRVGTAVVSLLLVLFVVWVMGRFVLNNQNKPTDQPVGAEELNLQTPATPLIMKAEPVL
                     YSGFGISRLAITHTDRPAQPRNSIIEYTIVAGDTLFGIAERFGLQPQTLLWSNRHILG
                     DDPHNIFPGVKILIPVMDGAVYKWNTGDGLNGVASFYHVTPDAIVDWPANQLNRETLG
                     DFAMPNIPEGTLLFVPGGVGEFTDWLPSYTREKPAESSISGSACGIQTSGYIGSGPPF
                     MWPTSETYISGYQYSPETNHRAIDIGGKIGNPVYAVDDGVVVYSNWNQSGYGNLIVID
                     HGNGWQSVYAHLDNYLSFCGDNVVRGQEIGILGTTGNSSGPHLHFELRHENYGAVNPW
                     DFLE"
     gene            4049..5857
                     /gene="metG"
                     /locus_tag="PLW25_07210"
     CDS             4049..5857
                     /gene="metG"
                     /locus_tag="PLW25_07210"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOS54310.1"
                     /translation="MTEFILVAAAWPYANAEIHVGNLCGSYLPADIFARYHRLRGRRV
                     SFVSGTDSHGTPITVRAEAEKSTPYQVYQRFHSGFLNLFQQLGLTYDLFTSTHTQNHM
                     HVARQVFLALKHNGFLYKESQDQWYSVSQQRFLPDRYVEGTCYICGYDGARSDQCDKC
                     GNMLEASQLLNPKSKIDGSTPELKSTEHYFLDLSRLQDLVVEFLKVRESYWRPNVLRQ
                     SLGQIQANGLRGRPITRDLEWGIKLPAEALGEGPEWENKCLYVWFEAVIGYLSASIEL
                     SQLSGEKDAWRNWWQDAAARTYYFIGKDNIPFHAIIWPAELLGAGEHLDELLGSSAPR
                     PLVLPYDVPANEFMNIESQKISGSHNWAIWGLDFLTRYDPDPLRYYLTVNMPESKDTD
                     WDWDDFYHRNNDELVATWGNLVNRVLSFTFKHWQGVVPDPGELTPLDLDLLRSMEDGF
                     TTVADELEAVRLRAALAEAMRLASEVNKYLDVTAPWQAVKTDKNAAGRAIFTALKAID
                     SLKILLAPFLPFTSEQLNGFMGYEGSLFGRQYTQPVSDELGEHLVLRYDHSCATGKWE
                     PSQLRPGQELRQPSPLFRKLEEKIVAEERARLGRPS"
BASE COUNT         1327 a         1630 c         1672 g         1357 t
ORIGIN      
        1 gcgcataata tgtggtgggt ttggaatcat gacgggcagc gcgctttcca gttcattgac
       61 ccgcgcctgt gggacctggt agcccacaat ccagtggctt ttttacgtcg tgtggagcgt
      121 caccgcctca acgccgcggt ggggcaatct gcctacctgg aacactacga ccgcaccctg
      181 gcgaactttg acgcttacat cgctgatcgt aatacctggt ttcagcgcac ctacccacaa
      241 tttacagata aaaccatcgc ctatttttcg ttcgagtttg gtattcacga gtctctaccg
      301 gtgtacgctg gcggtctggg catcctttcc ggtgatcatc tgaaagaagc ctctgacctg
      361 gggctgcccc tggtggggat gggttttgtg tacgacaagg gttattttgt gcaggaactt
      421 acggaagacg gctggcagga accgcgcaat tacctcatcg attacaatat gctgccactc
      481 attgcgctgc tggatgaaaa ccgtaaaccc ctcaccgtga ccatcgacct tcccgggcga
      541 caggtgagcg cccgcctgtg ggaactggaa gtagggcgca atcatcttta cctgctggat
      601 tcaaacgtgg agaccaatgc gcccgcggac cggcagctca ccgaacgcct ttacagtaat
      661 gaccttgaag tgcgcatctc gcaggagatg ctgctgggca tgggtggcgt tcaggcgctg
      721 cgcctgttgg gttacgaccc aggggtctgg catatgaacg aaggacactc cactttcctc
      781 gcgcttgaac gcatccgtga gaagatgaaa accggcacga gttttcaagc cgcctgggaa
      841 gaggttaagg ttactgatgt tttcaccacc cacacgcctg tgccagccgg taatgaccag
      901 ttcccgcttt ggctggtaga caagtacttt ggcaattatt ggcctgaact agggttaacc
      961 catgaccagt ttcttgatct cgggcgccag gcgcaatcat ggggcgatac ctttgtgatg
     1021 ccggtattgg cgctcaaact atcagaacgg gcgaacgcgg tttctgagct gcacggcatc
     1081 gtttcgcgca ggatgtggca cttcctctgg ccagaccgcg aggttgaaga tgtgccgatc
     1141 cactatgtga ccaacggggt gcatacgcgc tcgtggctgg caaggcgcat gggtgtgctt
     1201 tttgatacct acctgggcga agattggcgt gaacatctgg atgaacccga cttctggaac
     1261 aacatcaacc gtattcccaa tgaagaactg tgggcggtac gccgtcactt aaagaataag
     1321 ctggtgaact tcacgaatga acgcacccgc cagcagtggc agaacggtca gctcaccccc
     1381 agccaggcgg tcgccggggg cgtttttatg gaaccctatt cgctgacgat tggttttgca
     1441 cgccgctttg ccacttacaa gcgcgccaac ctggtattca aggatttcga tcgcctgatg
     1501 aatatcatca atgacgctca catgccggtg cagatcgtct ttgccggtaa agcccacccc
     1561 gccgacgagc ccggcaaact gctcattcag caggtctatc gcgcggtaaa agatacgcgc
     1621 agcgcaggtc gcctggtttt cattgaagat tatgacatta atgtcgcccg ttacctggtg
     1681 cagggtgtag acatctggct gaacacccct cgccgcccca acgaagcttc aggcacttcg
     1741 ggcatgaagg cggctctcaa tggtgtgctg aatttttcga ttttggatgg ctggtggcgc
     1801 gaagggtaca atggcaagaa cggctgggca atcggtaaag aaatcgagta cactgattct
     1861 gaagcacagg atgccgcgga cgcgcagagc ctttatgaaa cgcttgaaaa acagatcgtc
     1921 ccgctgtatt atgagcgcga tgagaacaaa atcccacaag aatggttaaa gatggtcaag
     1981 gagtgtttac gcacgctcgt accgcatttc agcctgcgcc gcatgttaaa agaatacacc
     2041 accgattact acctgccggc gatgaagcag gagaaagcgg aatggtaaga gaggaataaa
     2101 atggcatact tatctgaacc tcacgcctgg ctttttgcct tgatgatctt cgcgctgcgc
     2161 gttgtggata tgtcgttgga taccatccgc gtgctgttcg tggtgcgcgg gaaaaagctc
     2221 attgtttggg tgcttggttt ctttcaatcc ctggttttcg tcgtcgccat cagttcggtg
     2281 cttacacagt taaacaatat ccttaacgtc atcggctacg ccaccggctt cgccactggc
     2341 aacctggttg gcatgctcat cgagcagcgc ctggccatcg gccacatcct gatcactatc
     2401 atcagctcca gccgtggttc gtatatcgct gagcagttgc gcagcaatgg ctatgccgtc
     2461 accgaaatcg ccgggcgcgg cagagacggc accgtctttg agttgcacgc cagtgtattg
     2521 cgccgcgatg tccctcaagt agagaccatc gtactcgagg ctgacccaca ggcctttatc
     2581 accgccgaag acgtgcgccc ggtgcggcgt gggttctggc gggcttgagc ggtttacctt
     2641 gtatcgctta atgaaaaaga cccggggctt accatcccgg gtctttgtgc atgcatccgc
     2701 ctattccagg aagtcccagg ggttcacggc gccgtagttc tcgtggcgca gttcaaagtg
     2761 caggtgtgga ccactggagt tgccggtggt gcccagtatg ccgatctcct gtccgcgcac
     2821 cacattatcc ccgcaaaagc tgaggtagtt atcgaggtga gcgtagaccg attgccagcc
     2881 attgccatgg tcaattacaa tcaggttgcc atacccgctt tggttccagt tggagtaaac
     2941 aaccacgccg tcatccaccg cgtaaacagg gttaccaatc ttaccgccga tatcaatggc
     3001 gcggtgattg gtttcggggc tgtattgata cccggagatg tacgtctctg aagttggcca
     3061 catgaacggc ggaccagaac caatgtaacc agaggtctgt atgccgcagg cgctgcctga
     3121 gatggaagac tcggcgggtt tttctcgcgt gtagctgggc agccagtcgg tgaactcacc
     3181 cactccccct gggacgaaga gcagcgtgcc ttcagggatg ttgggcatgg cgaaatcgcc
     3241 cagcgtttcg cggttaagtt ggttggccgg ccaatcgacg atagcgtcag gggtgacgtg
     3301 atagaaactg gccacgccgt tcaggccatc acccgtgttc catttataaa cagcaccatc
     3361 catcaccgga atgagaattt tcaccccggg gaagatattg tgcgggtcat cacccaggat
     3421 gtggcgattg ctccataata aggtttgggg ttgcaggcca aatctttccg caatgccgaa
     3481 aagcgtatca cccgccacaa tggtgtactc gatgatgctg ttgcgcggct gcgccgggcg
     3541 gtctgtgtgc gttatcgcca gccggctgat cccaaacccg ctgtagagca ctggctcggc
     3601 tttcataatc aaaggtgtgg ctggggtttg caggttcagt tcttcagccc ccactggctg
     3661 gtcggttggt ttgttctggt tgttaagcac gaacctgccc atcacccaca ccacgaagag
     3721 caccagtaag agggagacta ccgcggtgcc cacgcgtaaa gtggtttcgc ccagcccgag
     3781 gtgcaggatg cgattccata acctctctgc tggcgaaaaa gcctcgtcgg cggggttttc
     3841 tgataattgt tgagcctcgc ccgcagtatt tttatctgag ttgtccatca tatttacctc
     3901 gaaaaaaatc ataacatcgc gcatcaatat ggtcaagttt tgaaaaatcg aatgggccga
     3961 gggtttggag gtgagattaa ctgcaagggc gggtgcgccc gcgccaggtt tcgtgttaga
     4021 ataccgccat caagtaagcg aggtgagcat gacggaattt atccttgttg cagcggcgtg
     4081 gccttacgct aatgctgaaa ttcacgtggg caacttgtgc ggttcttacc tgccagcaga
     4141 tatcttcgcg cgctatcacc gcctgcgcgg acgccgggtg agtttcgtct ccggaacgga
     4201 tagccacggc acacccatca ccgtccgcgc cgaagcggaa aaaagcaccc cctaccaggt
     4261 gtaccagcgc ttccacagcg ggtttctcaa cctctttcag cagctcggcc tgacttacga
     4321 tctgttcacc agtacgcata cccaaaatca catgcacgtg gcacgccagg ttttcctggc
     4381 attaaaacac aacggcttcc tttataaaga gagtcaggac cagtggtatt ccgttagcca
     4441 gcagcgcttt ctgcctgatc gctacgtgga gggtacctgt tatatctgcg ggtatgatgg
     4501 cgcgcgcagc gaccaatgcg acaaatgcgg caacatgctc gaagcctcgc agctgctcaa
     4561 ccctaaatcc aagatcgacg gcagcacccc ggagttgaag agcaccgagc attacttcct
     4621 ggatctttcc cgcctgcaag acctggtggt ggaatttctt aaagtacgtg agagttactg
     4681 gcgccccaac gtgctgcgcc agtcgcttgg gcaaatacag gcaaacggtc ttcgcgggcg
     4741 tcccatcacc cgcgaccttg aatgggggat taagcttccc gccgaagccc tcggagaggg
     4801 tccggaatgg gagaacaagt gcctttacgt atggtttgaa gcggtgattg gttacctgtc
     4861 cgcttccatt gagttgagcc aactcagcgg agagaaagac gcctggcgta actggtggca
     4921 ggatgccgcg gcgcgcactt attattttat cggtaaagat aatattccat ttcatgccat
     4981 catctggccg gcggaactgc tcggcgccgg cgaacacctg gatgaattgt taggcagcag
     5041 cgcacccagg ccgttggtgc tgccttatga tgtgccagca aatgaattta tgaacattga
     5101 atcccagaag atttccggca gccacaactg ggctatctgg gggctggatt tcttgacgcg
     5161 ctatgaccct gacccgctgc ggtactacct gacggtcaac atgcctgaaa gcaaggatac
     5221 cgactgggat tgggatgatt tctatcaccg caacaacgat gagctcgtgg ccacctgggg
     5281 caacctggtc aaccgcgttc tctctttcac cttcaaacac tggcaggggg tggtacccga
     5341 cccaggtgag ctgactcctc tggacctgga cctactgagg agtatggaag atggcttcac
     5401 caccgtcgcg gatgaactgg aagcggtgcg attgcgcgcc gctctggcag aagccatgcg
     5461 cctggcttct gaggtcaaca agtacctgga tgtgaccgct ccctggcaag cagtcaagac
     5521 ggataaaaac gccgcgggca gggccatctt cacagcgtta aaagctatcg attcattaaa
     5581 aatccttttg gcgcccttcc tgccatttac cagtgaacaa ttaaatggct tcatgggcta
     5641 cgagggcagc ctgttcggca ggcagtacac gcaaccggtc agtgatgaat taggtgaaca
     5701 cctcgtgctg cgttacgacc atagctgcgc cactggaaag tgggagccca gccagttgag
     5761 accgggacag gaattgcgtc agccatctcc ccttttccgc aagctggagg agaagatcgt
     5821 ggctgaagaa cgcgcccgcc tgggaagacc ttcttaaaca attgagtgaa ccggaggaaa
     5881 ctccggtttt ttttatccgt tctgaaaggc gcagcgaatc cagcccggca gcgcctgcag
     5941 cagcgggcgc atgacctggg cggcacgagt gttgaaatct ccccca
//
LOCUS       DAOSPM010000079         5577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_14692_length_5577_cov_12.787758, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000079 DAOSPM010000000
VERSION     DAOSPM010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14692_length_5577_cov_12.787758"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..694
                     /gene="thpR"
                     /locus_tag="PLW25_07215"
     CDS             104..694
                     /gene="thpR"
                     /locus_tag="PLW25_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOS54311.1"
                     /translation="MQTNIRAFIAIPLTREIHQQLGLFIQSYGLANRDAGFRPVKPEN
                     IHLTLKFLGDVKAASLPAISEHLDRLTVTLKQTSITISGLGAFPGWFKQPRVIWVGVQ
                     PVEPIQRMSEQIEKAISALGFPAERKPFSPHLTLARVNQQVAGQRYQEVITRLRNLQP
                     APVFGDMVADKIILFKSDLQPSGPVYSILSAHRLKG"
     gene            768..1085
                     /gene="rsfS"
                     /locus_tag="PLW25_07220"
     CDS             768..1085
                     /gene="rsfS"
                     /locus_tag="PLW25_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOS54312.1"
                     /translation="MVNFLEEKKAEKIILLDIRELTTFTDYFIICNGTSDRMLQALAT
                     TLRDYIKKDLELIVNIEGESRDGWLVADLEDIVVHIFSPEMRNYYRLEQLWNKGKTIL
                     TLQ"
     gene            complement(1121..1573)
                     /gene="bcp"
                     /locus_tag="PLW25_07225"
     CDS             complement(1121..1573)
                     /gene="bcp"
                     /locus_tag="PLW25_07225"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOS54313.1"
                     /translation="MLKENTQAPDFSLPDETGVIHTLSQYAGRNIILYFYPKDDTPGC
                     TIEACNFRDDYSAYENSQVVILGVSPDSPKSHARFKAKYHLPFSLLSDEEHKVLEMYG
                     VWGKKKMFGKEYHGVLRTTYLIGKDGKIIKVFKNVKPAGHSAEILANL"
     assembly_gap    1797..1896
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1897..2204)
                     /locus_tag="PLW25_07230"
     CDS             complement(<1897..2204)
                     /locus_tag="PLW25_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54314.1"
                     /translation="MTKRKRILRDFKVYWVLPLIFTSMLGVSLVHYFGGNLNGISIIL
                     GGMVCLSLFAMRIFLNAYFDHPDSPISALSREDEQWEKLKEVKRNLLLQYSLLSLTAG
                     A"
     gene            2310..3242
                     /locus_tag="PLW25_07235"
     CDS             2310..3242
                     /locus_tag="PLW25_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOS54315.1"
                     /translation="MKLDLPDIYLVRPDVRQDLSNGQPVVALESAVITHGLPRPANLE
                     LALSMEAIVRGQGAIPATIAFIDGKIVIGASESELRRLAEENNPIKINPRNIGIALNS
                     GACGGTTVAATLMAARAAGIKVFATGGIGGVHRGNVFDISADLPALSSNPLVVVCAGA
                     KAILDIPATLENLETRGVPVLGFQTNEFPGFYSPGSGFKVDYRIESALEAFKIASAHW
                     RAGCRSAVLVCNPIPAEYGVDQQKIDDAIAQAVESANRKGISGAALSPFLLEELGKIT
                     AGKSLQANLALLKNNALLAAQIAGFFAKPGQISF"
     gene            complement(3245..4075)
                     /locus_tag="PLW25_07240"
     CDS             complement(3245..4075)
                     /locus_tag="PLW25_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54316.1"
                     /translation="MNIDVATTVKVILVLLGIAAIVSLTMAVKSIKAGYRLLFFQKRQ
                     LLVAHGWRLVLLALGLLLTGLVLIRFGEPMVYRYFPPSPTITQTPTVTITPTITNTPS
                     MTYTPTITLTLSQTYTPSLPEFIQATIQTPVGPDANAIFSPLSFSDTIKDGVVTTSYS
                     EFELPVGTLFGGFSYDRMAIGAQWTALWFYGDELICSETKAWNYAPGGYGYTDCSRPV
                     DAWRPGIYEVRIFVGQTWKQSGTFKILGEEPLVEKIPTSMGAQAPVESAVTATPPSTP
                     "
     gene            complement(4139..5101)
                     /locus_tag="PLW25_07245"
     CDS             complement(4139..5101)
                     /locus_tag="PLW25_07245"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HOS54317.1"
                     /translation="MKIPRVPIAHLPTRIEYLPRLTDALGGPQLYCKRDDQTGLGFGG
                     NKTRKLEFLLAEARAQNADTLVSVGAIQSNHCRQVAALSARFGYQCILVLSGDEPALA
                     SGNLLLDQLFGAKIVWSTRDNRLQMLEDIVKTAQHEGRRPYKIPYGGSSAVGLMGYVE
                     AMKELELFPEKFDWIVIPSSSGGTQAGMLLGAKGAGFTGKILGICIEGNNRELHSTVA
                     SLMREGAELCGLPPVSEDTILVNDHYVGGGYGIMGTPEIEAIKLLALTEGLLLDPVYT
                     GRAAAGLIDLVRKGFFNKTDRVLFWHTGGTPALFAEPYLSLLQH"
     gene            complement(5098..>5577)
                     /locus_tag="PLW25_07250"
     CDS             complement(5098..>5577)
                     /locus_tag="PLW25_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOS54318.1"
                     /translation="ARRDNFIIRSEAYYLDVWSRFMQAGMAEGLIAEFAGQPIAGMVL
                     MFFGSRAWFVYGMSTSQHREKMPNYLLQWEAMRLAHEKGCTTYDLWGAPDTPDPSDPM
                     FGVYRFKEGLGAELVYTIGAWDFPLKPALYRLYHHVIPRVLSITRYVRRKKLTQEVI"
BASE COUNT         1387 a         1467 c         1332 g         1291 t
ORIGIN      
        1 acagcagggt atcgttctta taccatgatt taggtaaaat gggtagataa gccatatcac
       61 gtctctttcc gcatagaaaa accaggtaga atgagaacca gagatgcaaa ctaacattcg
      121 tgccttcatc gccattccgc tcacccgcga gattcatcaa caacttggct tgttcatcca
      181 aagctatggg ctcgcgaacc gcgacgccgg ttttcgcccg gtaaagccag aaaatatcca
      241 cctgacccta aaattcctgg gggatgtaaa agcagccagc cttcccgcca tcagtgagca
      301 tttggatcgc ttgacagtaa ccttgaagca gacctccatc accatcagcg gcttgggtgc
      361 ttttcctggt tggttcaaac aaccccgcgt tatttgggtg ggagtgcagc cggttgaacc
      421 gatacagcgc atgagcgaac aaatcgaaaa agcgatatct gcgcttggct ttcctgcgga
      481 gcgcaaaccc ttttcccctc atctcacgct tgcccgcgtt aaccagcaag ttgccggtca
      541 acgttatcaa gaggtcatta cccgcctgag gaacctccaa cccgcgcccg ttttcggtga
      601 tatggtcgca gataagatca tactgttcaa atctgacctt caaccctcag ggccagttta
      661 ttccatcctt tcggcgcata gattaaaagg ttagaacaac tatgttagaa ttcagaaaat
      721 cgatcattga gaggtgaatc ctggatccaa tcgaaaaagc acatcgaatc gtcaattttc
      781 tcgaagaaaa aaaggctgaa aagattatct tgctggatat ccgtgaactg accactttta
      841 cagattattt catcatttgc aacggaacca gcgatcgtat gctgcaagca ctggctacaa
      901 ccctgcgtga ttacattaag aaagacctgg agctgattgt caacatcgag ggagaatcac
      961 gagatggctg gctggtggcc gaccttgaag atatcgtggt gcatatcttc tcgcccgaaa
     1021 tgcgaaatta ttaccggctg gaacagctgt ggaataaagg caaaacgatc ctcacgctgc
     1081 agtaaaaaaa gcgtaagacc tcccctcagc tttgccgtct ttacaggttt gccaggattt
     1141 cggcgctgtg gccagcgggt tttacatttt taaatacttt gataatcttc ccgtctttgc
     1201 cgataaggta ggtggtgcgt aaaacaccgt ggtattcctt gccgaacatt tttttctttc
     1261 cccagacgcc atacatttcc agcactttat gctcttcatc agaaagcagt gaaaacggta
     1321 agtgatattt ggccttaaac ctggcgtgtg atttgggaga atcagggctc acccccagga
     1381 taaccacctg gctgttttcg taagcgctgt aatcatctct gaagttgcag gcttcaatgg
     1441 tgcatcctgg ggtgtcgtcc ttcgggtaga aataaaggat aatattccga ccagcgtatt
     1501 gggagagtgt gtggataaca ccagtttcat ctggcagtga aaaatctgga gcctgcgtgt
     1561 tttctttaag catggtattc ctcctgttcc tgtctgatta tatcaaatcc cttcaaccac
     1621 catcagaacc atagcggcac ctggattaag tagatcatca tcagtaccat ccctattgcc
     1681 aaccagagca tgattttcca ggctggttta tcaccttccg ccacgcgcag cacctgccat
     1741 acctgaaaaa tcccaaatgg gagaaccatc cacgctggcc aggccagtga ccagggnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgc cagcggttaa actcaataat
     1921 gagtactgaa gcaagaggtt gcgcttgacc tctttcaatt tttcccattg ttcatcttcg
     1981 cgactcagcg cggagatcgg agagtccgga tggtcaaaat aggcgttcaa gaagatgcgc
     2041 atcgcgaaca acgacaggca aaccatgcca cccaggataa tactgatccc attcaggttg
     2101 ccgccaaagt aatgcaccag gcttacgccc agcatgctgg taaaaatcag cggaagaacc
     2161 caataaacct taaaatcccg caatatacgc ttacgctttg tcacttgctg ccccggggtc
     2221 aattgtagtc tcctgccttt tttccgaata aggatgccct attcaccatt ttaatatata
     2281 ctgcactata tcacagccgg agactgacca tgaaactgga cctcccagat atttatctcg
     2341 ttcgccctga tgtgcgccaa gacttatcaa atgggcagcc agtcgttgcc ctggagtcag
     2401 cagtcatcac gcatgggcta ccccgccctg ccaacctgga actcgccctc tcaatggaag
     2461 cgattgtccg cggtcaggga gccatccccg ccaccatcgc gttcattgac ggcaagatcg
     2521 tcattggcgc cagcgaatct gaactccggc gcctggcaga ggaaaacaac cccatcaaaa
     2581 ttaacccgcg caatatcggg atcgctttga acagcggggc gtgcggcggt acgaccgtcg
     2641 ccgcaacttt gatggctgcc cgcgctgctg gcattaaggt cttcgctacc ggcggaatcg
     2701 gcggagtgca tcgtggaaat gtttttgata tttcagccga tttacctgca ctatccagca
     2761 acccgctcgt cgtcgtttgc gcgggtgcca aagccattct ggatattcct gccacgctgg
     2821 aaaacctgga aacccgcggg gtgcctgtcc tgggtttcca gacgaatgaa ttcccggggt
     2881 tctattcacc aggcagtggt tttaaagtgg actaccgtat tgaatctgcg ctggaagcct
     2941 ttaaaatcgc ttcggctcat tggcgtgccg ggtgccgcag cgcggttctg gtatgcaacc
     3001 ccattccggc ggaatatggg gtagaccagc agaaaatcga tgacgcgatc gcccaagccg
     3061 ttgaatccgc caaccgcaag gggatctcag gcgcggcact ctctccattt ctgttggagg
     3121 aactgggcaa aatcacagcg gggaaaagcc tgcaggcaaa cttggcgcta ttaaagaata
     3181 atgcgctgct cgcagctcaa atcgccggat tctttgcaaa accaggtcaa atcagttttt
     3241 aacttcaagg agtggacggc ggggttgcgg ttactgcgct ttcaacaggt gcttgtgctc
     3301 ccatgctcgt tggaattttt tcaaccaagg gttcttcccc gaggatcttg aaggtgcctg
     3361 actgtttcca ggtctgtccg acaaaaattc gcacctcgta gatccccgga cgccaggcgt
     3421 ccacagggcg tgaacaatcc gtataaccat aacctccggg agcataattc cacgccttgg
     3481 tttctgaaca gatcaactca tctccataga accataaagc cgtccactgc gctccaatag
     3541 ccatacggtc ataagaaaaa ccaccaaata acgtccccac gggtaattca aactcactgt
     3601 aagaagtcgt caccacaccg tcttttatgg tgtcagaaaa actcaatggg ctgaaaatcg
     3661 cgttcgcgtc cggtccgacg ggggtttgaa tggtcgcctg gatgaattca ggcaatgaag
     3721 gtgtgtaagt ttgagataaa gtcaaggtga tggtcggggt ataggtcatc gaaggcgtat
     3781 tggtgatcgt aggtgtgatg gtcacggtag gcgtttgggt gatggtagga gaaggaggaa
     3841 aatagcggta gaccatcggt tctccaaagc ggattaatac cagccctgtg agcagcaacc
     3901 caagcgcgag cagcaccagg cgccagccgt gagcgaccag taattgacgc ttctggaaaa
     3961 aaagcagcct gtaacctgct ttgatggatt taactgccat tgtcaggcta acgatggctg
     4021 caatccccag caaaaccagg atcactttta ccgttgtggc aacatcaata ttcaatgctt
     4081 ctctccaaaa aagcattata gcatgcgcaa aaaaccagct cggggcagag aggtggtttt
     4141 agtgttgtaa cagcgacagg tatggttctg caaataaggc aggtgtgccg ccagtatgcc
     4201 agaaaaggac cctatccgtt ttattaaaaa agcccttcct gaccaggtct atcaggccgg
     4261 ccgcggccct tccggtgtaa actgggtcca acagcaatcc ttcagtcaaa gccaggagct
     4321 taattgcttc aatttccgga gtgcccatta tcccgtaccc cccgccaaca taatggtcgt
     4381 ttaccaggat cgtatcttca ctcactggag gtaaaccgca caattccgca ccctcgcgca
     4441 tcaggctggc gacagtgctg tgtaattccc ggttgttgcc ttcaatacag attcccagaa
     4501 tcttgccggt aaagcctgcc ccttttgctc ccagcagcat gcccgcctgg gttcctccgc
     4561 tggatgaggg aataacaatc cagtcaaact tctctgggaa gagctcgagt tctttcatgg
     4621 cttcaacata ccccatcaag cccaccgcgc ttgagccacc ataagggatt ttataagggc
     4681 gtcgaccctc atgttgcgct gtcttgacga tatcctccag catctgtaaa cggttgtcac
     4741 gcgtcgacca cacaattttc gcgccaaaaa gctgatccag taaaagattc ccactcgcga
     4801 gagcaggttc gtctccagac aacaccagga tgcactgata accgaatcgg gcagacagcg
     4861 ccgccacctg ccggcagtga ttggattgga tggctcccac gcttaccagg gtgtccgcgt
     4921 tttgagcgcg cgcttcagcc aacaaaaact ccagtttgcg cgtcttgttt ccaccaaaac
     4981 ccaacccggt ctggtcatcg cgcttacaat acaattgtgg gccacctaaa gcatcggtca
     5041 ggcgtggcag gtattcaata cgggtgggta gatgagcgat cggcactctg gggattttca
     5101 tatgacctcc tgagtcaatt ttttccggcg gacatatctg gtaatcgaaa gcacgcgcgg
     5161 aatgacatgg tgataaaggc gataaagcgc aggtttaaga gggaaatccc aggctccaat
     5221 ggtataaacc agttccgcgc cgagcccttc tttaaaacgg tacactccaa acatcgggtc
     5281 agatggatct ggtgtgtcag gcgcgcccca caggtcgtac gttgtgcagc ctttttcgtg
     5341 cgccaggcgc atggcttccc attgcagcag atagttgggc atcttttcac ggtgctggct
     5401 ggttgacatg ccatagacga accaggcgcg cgagccaaaa aacatcaaca ccattccagc
     5461 gatgggttgc cccgcgaatt cagcgatcaa cccctccgcc atacccgcct gcatgaagcg
     5521 cgaccacaca tccaggtaat aggcttcaga ccgaatgatg aagttgtcgc gccgcgc
//
LOCUS       DAOSPM010000080         5519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_14863_length_5519_cov_16.733529, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000080 DAOSPM010000000
VERSION     DAOSPM010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14863_length_5519_cov_16.733529"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..76)
                     /locus_tag="PLW25_07255"
     tRNA            complement(5..76)
                     /locus_tag="PLW25_07255"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Lys,seq:ttt)
     gene            complement(215..1285)
                     /locus_tag="PLW25_07260"
     CDS             complement(215..1285)
                     /locus_tag="PLW25_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOS54319.1"
                     /translation="MTRMFVPGPVDVAPEVLEAQTKPMLPHRSKDFEAIFRGTEEKAR
                     QLFYTQFRVYQAASSGSGMQEAGIRNFVQKDVLSCINGAFAQRWYDVAVANGKTADKL
                     EVEYGEAITPELLRDALKQKHYEAVTIVHNETSTGVENPLADLCAVVKEISPDTLILV
                     DAVSSLGGVKIEMDKLGVDFLLTSSQKCLALPPGLALAAATDRALAKAENVSNRGWYF
                     DLLLMEKHRVKDSTPMTPAMALIFALDRQLDRILAEGLDARFARHAAMARRVQEWASG
                     HGMEPLAKPQYRSKTVVTIKNSLNWDISALNKFLQTRGMRIANGYGKIKDLTYRVATM
                     GETSMADIEALLVAMEDYMKQS"
     gene            complement(1346..1558)
                     /locus_tag="PLW25_07265"
     CDS             complement(1346..1558)
                     /locus_tag="PLW25_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54320.1"
                     /translation="MAKKVKKRSTNAPAAVVAKTETATPKAAASRTPTAEFDPDYSFV
                     KKDLKRIGILAGSFFVVLVVLSFFLR"
     gene            complement(1585..2421)
                     /locus_tag="PLW25_07270"
     CDS             complement(1585..2421)
                     /locus_tag="PLW25_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HOS54321.1"
                     /translation="MEKSRVEREARLEAFRKAGRLIEAPLPAEGETFEPAIEAEEYTA
                     PRARRTTRRRTGLDRFLFAVEIIAIIGLLFIIFNGVKLMRELNQQVASVIEQPTLTPT
                     PLITAVVLPGGHVPPVSGQENRFNEAEIPEHLRPLMQSYANLPVPTQSPQQARQIQIP
                     AIGVSAPVVLGDGWEQLKKGVGQHIGTANPGDKGNLVLSAHNDIFSEIFRDLDKLKKG
                     DEVTIITQDRSFTYVITSSQIVEPTRVDLLAQTSDSMITLISCYPYLVDNKRIVVQGI
                     LQ"
     gene            2608..3372
                     /locus_tag="PLW25_07275"
     CDS             2608..3372
                     /locus_tag="PLW25_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOS54322.1"
                     /translation="MKKNYLIDMDGVLVTGKTPLPGAVEFIDRLLACKVKFLILTNNP
                     LYTQRDLEHRLNTIGLNVPAERIFTSAIATARFLQSQKPNGKAFVIGESGLTQALHDV
                     GYVQTDLSPDYVVLGEVTNYNFELITKAVRLINDGAMFIATNPDSTGPLEAGIVPATG
                     ALAALIQKATGREPFFVGKPNPLMMRSALRYLGVHSEDTVMIGDRMDTDIVAGVMSGL
                     ETILVLSGVSTREDVERFPYRPTQILNSVGEIKIAC"
     gene            3394..4332
                     /locus_tag="PLW25_07280"
     CDS             3394..4332
                     /locus_tag="PLW25_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOS54323.1"
                     /translation="MNTKTESKTQQRFIISIILTGLILVAEIIGGLLSGSLALLSDAA
                     HVFSDIFALVLSYFALRLVARPPDERHTYGWHRAEVIAALINGASLLVIAVGIWVEAV
                     KRWQAPLEIKSTEMMIIAVIGLVVNIIVAVILGGHDHTHEHGHDHEGHAEKREKRNLN
                     VHSAYLHVLGDLISSIGVIIAALLIRLTGERWIDPLISVLIGAIILVSAYRLLRKSLH
                     ILIEGVPEGLSLQQLHQSIASVGEVDTVHDLHVWNLGSNQVALSAHIVLSAEFFSQQP
                     LVLEKIKSLLAAEYDIHHTTLQFEETRCGEGHGGCN"
     gene            4501..5418
                     /locus_tag="PLW25_07285"
     CDS             4501..5418
                     /locus_tag="PLW25_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOS54324.1"
                     /translation="MRWVRETVPQPDQNRYYRIAMSITLGGNLLLAVGKGVAAWLSGS
                     SAVYSDAANSVSDVVYSLLMVLGLWVATRPPDLSHPQGHSRFEPLVALMVAAAMALAG
                     IEAARTAYVRYTEPGLSAIRLDLPTWVLLASALVKAAMYASITRISKRLHSPALATSA
                     RDNLSDVLSSLAAFVGVLGSNYIHPLADPVAGFLVAVWILRNAFLAGRENLGFLTGKG
                     ASQEKINQFVTAAESVPGVIKVHHIVTDYVGPRLMLDLHINVDGNSTVNHAHEISDEV
                     TKRLESFPEVDRAYVHIEPHDWEDDKSPT"
BASE COUNT         1175 a         1559 c         1482 g         1303 t
ORIGIN      
        1 aaagtgagcg cggaggggat cgaaccctca accaacggct taaaaggccg ctgctctgcc
       61 attgagctac gcgcccaacc tcaaacgcaa agattttatc acgcccgttg acaatggtca
      121 atcattttcc gcgctttcgc tccaggcttt ccaggctgcc caggcgcgcg aaaaaaaggc
      181 cggtcgccca gccttttcgg tttcctgtat agggttagct ctgcttcata taatcttcca
      241 tcgccacgag caaagcctcg atatccgcca tgctggtttc gcccatggtg gccacgcggt
      301 aagtcaggtc tttgatcttg ccgtacccgt tggcaatgcg catgcccctg gtttggagga
      361 acttgttgag cgcgctgata tcccaattca ggctattttt gatggtcacc acggtcttgg
      421 agcggtactg gggttttgcc agcggctcca tgccatgccc actcgcccat tcctgcaccc
      481 tcctggccat ggccgcgtgg cgggcgaagc gggcgtcaag cccctccgcc aggatacggt
      541 cgagctggcg gtccagggca aagataagcg ccatcgccgg ggtcatgggg gtggagtctt
      601 tgacgcggtg tttttccatc aacagcaggt caaaatacca gccacggttg ctcacatttt
      661 ctgcttttgc cagcgcgcgg tctgtggcgg ctgccagcgc cagcccgggc gggagagcca
      721 ggcatttctg cgaactggtc agcaaaaaat ccacgcccag tttgtccatc tcaattttca
      781 caccgcccag cgaagagacc gcgtccacca aaatgagcgt atcggggctg atctctttca
      841 ccaccgcgca caggtctgcc agtgggttct caacgccagt ggaagtttca ttatgcacaa
      901 tggtgacggc ttcgtaatgc ttctgtttta aggcgtcacg cagcagttca ggagtgatgg
      961 cctcgccata ttcaacttcc agtttatcgg cggttttccc gttggctacg gccacatcgt
     1021 accagcgctg cgcgaaagca ccgttaatac aggagagcac gtctttttgc acaaaattgc
     1081 ggatacccgc ttcttgcata cctgaacccg atgatgccgc ctggtatacg cggaactggg
     1141 tatagaataa ctgcctggct ttttcttctg tcccacgaaa aattgcttca aaatctttgc
     1201 tgcggtgtgg aagcatcggt ttggtttggg cttccagcac ctccggcgcc acatcaactg
     1261 gtcctggtac gaacatacgg gtcatacgct tctccttatt aagttggttt attggatccg
     1321 gaagttgccc ttccgggtag tacccttaac gcagaaagaa agacagcacc accaaaacca
     1381 caaaaaagga cccggccaga atgccaatgc gcttgaggtc tttcttgaca aaagagtaat
     1441 cggggtcgaa ttctgcggtt ggcgttcgcg atgctgcagc cttaggggtt gctgtttccg
     1501 tttttgccac caccgcggca ggtgcgtttg ttgagcgttt tttaaccttt tttgccattt
     1561 tattctcctg ttacgtaatt ataatcactg caaaatcccc tgcaccacga ttcgcttgtt
     1621 atccacaagg tacggatagc aggagatcaa tgtgatcatg ctgtcgctgg tctgcgccag
     1681 cagatcaact cgagtaggtt ccacaatctg gctgctggtg atgacgtagg tgaaggacct
     1741 atcctgtgtg ataatggtta cctcgtcacc tttcttcaac ttgtccaggt cgcggaagat
     1801 ctcgctgaag atatcgttat gcgccgaaag caccaggttg cccttatctc cggggttggc
     1861 cgtgccaata tgctgtccca cgcccttctt caactgctcc cagccatctc ccaacaccac
     1921 cggtgcgcta acaccaatcg cggggatctg tatctgacgc gcctgttgag ggctttgcgt
     1981 gggcacgggt aggttggcgt aactctgcat cagcgggcgc aggtgttcag ggatttctgc
     2041 ttcgttaaag cggttctcct gcccggatac tgggggcaca tgcccccctg gcaacaccac
     2101 cgcggtgatc agcggcgtgg gggtcagcgt gggttgctcg atgaccgatg ccacctgttg
     2161 gttcagctcg cgcatgagct tcaccccatt gaaaatgata aagagcaagc caatgatggc
     2221 gatgatttcc accgcgaaga ggaagcgatc gagaccagtg cgccgcctgg tggtgcgacg
     2281 ggcgcggggg gcggtatact cctcagcctc gattgccggc tcaaatgttt ctccctcagc
     2341 aggcagaggg gcctcgatca gcctgccggc tttcctgaac gcctccagcc tggcttcacg
     2401 ttccacgcgg ctcttctcca tgagaagctg cctgagttcg ctggctgtgt aatcttcaat
     2461 cgcgcgccgc cgtggcactg gtttacctct tcttgttgga accaacgcgt tgattataac
     2521 cgatttacgc accaggcttt ctttgcaaaa tacaacaacc cggtcatccg tgttaaaatg
     2581 tacccgtcaa tgaaaagagg aatgcgcgtg aagaagaact atcttatcga tatggacggc
     2641 gtgctggtaa ccgggaagac cccactcccc ggcgccgtgg aatttatcga ccgcctgctc
     2701 gcctgcaaag tgaaattctt gatcctcacc aataacccgc tctatacgca acgcgacctc
     2761 gagcaccgcc tcaatacgat cggtctcaac gtgccggctg agcggatttt cacctcggcg
     2821 atcgccaccg cgcgtttctt gcaaagccag aagcccaacg gcaaggcttt cgtcattggt
     2881 gaaagcgggc tcacgcaagc gctgcacgac gtcgggtatg tgcaaaccga cctcagccct
     2941 gattacgtgg tgctgggtga agtgaccaat tacaattttg aattgatcac caaggcggtt
     3001 cgcctgatca atgatggggc gatgttcatc gccaccaatc ctgattcaac cggcccgctg
     3061 gaagccggta ttgtgcccgc caccggcgcg ctggctgcgc tcattcaaaa agccaccggc
     3121 agagagcctt tttttgtagg caaacctaac ccactgatga tgcgcagcgc cctgcgttac
     3181 ctcggcgtgc attctgaaga taccgtcatg attggcgatc gcatggacac agatattgtc
     3241 gcgggcgtga tgagcgggct ggagaccatc ctggtgttga gcggtgtttc cacccgtgag
     3301 gatgtggagc gcttccctta ccgccccacg caaattctca actcggtagg cgagataaaa
     3361 attgcctgct gaggcacgcg ctcggagttc acaatgaaca cgaaaacaga aagtaaaacc
     3421 caacaacgtt ttatcatttc gatcatcctt accggcctta ttctggtcgc tgaaattatc
     3481 ggcggtttat tgtctggctc gctggcgttg ctttcagacg ccgcgcatgt gttttcagat
     3541 attttcgcac tggtattgag ctacttcgca ctgcgcctgg ttgcgcgccc cccagatgag
     3601 cgtcacactt acggctggca ccgcgctgaa gtgatcgccg ccctcattaa tggcgcttcc
     3661 ttgctcgtca tcgccgtagg catctgggtg gaagcagtca aacgctggca ggctccgctg
     3721 gaaatcaaga gcacggagat gatgatcatc gccgttattg gcctggtagt gaacatcatc
     3781 gtcgctgtca ttcttggcgg gcacgatcat acgcacgagc acgggcacga ccatgaagga
     3841 cacgcagaaa agcgcgaaaa acgcaacctg aatgtgcata gtgcttacct gcacgtgctg
     3901 ggcgacctca tttcttctat cggtgtcatc atcgcggcgc tgctcatccg gctgacgggc
     3961 gaacgctgga tcgacccgtt aatctccgtc ctcatcggcg ccatcatcct ggtcagcgcc
     4021 tatcgcctgc tgcgcaaatc cctgcatata ttgattgagg gtgtgcccga agggctttct
     4081 ttgcagcaat tacaccaatc catcgcctcc gtcggcgagg tcgatactgt tcacgacctg
     4141 catgtgtgga acctgggttc gaaccaggtg gcgttgagcg cccatattgt actctcggcg
     4201 gagtttttct ctcagcaacc attggtgttg gagaagatca aaagtctgct tgccgctgaa
     4261 tacgacattc accacaccac cctgcaattt gaagaaacgc gctgtggtga aggccacggc
     4321 ggctgcaatt aagacctcaa agccagcctg aaaaaccagg ggatgtgttt ttactcaatg
     4381 cgaacaacca ggcagggctg acagcctgga aatgagcatg aattaacgat aaaggtggaa
     4441 accctatcac ggatgaaagc attgcatctt caagatgttg attatcagga gaaacgttga
     4501 atgcgctggg tgagagagac agtcccacag cctgatcaaa atcgttatta ccgtatcgcc
     4561 atgagcatca cacttggcgg taatttgctg ctggccgtgg gaaagggggt ggcggcctgg
     4621 ctatcaggtt catccgcggt ttattctgat gccgccaact cggtttcaga cgtggtctac
     4681 tcgttattga tggtgctggg tctgtgggtt gccacacgcc cgcctgatct tagtcatcct
     4741 cagggacatt cacggtttga acccctggtc gccttaatgg ttgccgccgc gatggctttg
     4801 gcaggcatcg aagcagcgcg taccgcttat gtccgttaca cagagccggg tctgagcgcc
     4861 atccgcctgg atttgcccac atgggtattg ctggcaagcg cgctggtcaa agccgcgatg
     4921 tacgcatcga tcacgcgcat cagcaagagg cttcacagcc ccgcgctcgc caccagcgcg
     4981 cgcgacaatc tcagcgatgt cctgtcttcc ctggcagcct ttgtgggggt tctgggttcc
     5041 aattatattc accccctggc agacccggtg gcggggttcc tggtggctgt ttggatcctg
     5101 cggaacgcct tcctcgccgg gcgggagaac ctgggttttc tcaccggtaa gggcgcgtcg
     5161 caagagaaaa tcaaccagtt tgtcaccgcc gctgaatccg tgcctggtgt gatcaaggtg
     5221 caccacatcg tcaccgatta tgtgggtccg cggttgatgc tcgatttgca tatcaatgtg
     5281 gacggcaaca gcaccgtaaa tcacgcccac gaaatatccg atgaagtcac gaaaaggctc
     5341 gagtcctttc ctgaggtcga ccgcgcctat gtgcacattg aaccgcatga ctgggaggat
     5401 gacaaatcgc ccacttaatc aaacacattc tgcgattggt ttttccatgg atcagcgcca
     5461 ggtgaaaggt gaagaatgaa aacccgcccg gcccaattca tcgatgagcc catccaggt
//
LOCUS       DAOSPM010000081         5347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_15455_length_5347_cov_15.027778, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000081 DAOSPM010000000
VERSION     DAOSPM010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15455_length_5347_cov_15.027778"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1167
                     /gene="cfa"
                     /locus_tag="PLW25_07290"
     CDS             52..1167
                     /gene="cfa"
                     /locus_tag="PLW25_07290"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HOS54325.1"
                     /translation="MASAESVVRELLDLAQVKVNGPDPWDIQVHNPAFYDRVLRDGTL
                     GLGESYMEGWWDCPAIDQFITRALLARLDEKVKNESRFLLQALRSRLFNRQSRGRAFE
                     VGIKHYDLGNDLYQAMLDKRLVYTCGYWKDAKNLDDAQEAKLDLVCRKIGAHPGMRIL
                     ELGCGWGSFAKFAAEKYGVEVLGVTVSKEQVALGMELCKGLPVELRLQDYREVSGTYD
                     AVISIGVMEHVGYKNYRDYMLVVDRCLKPEGIGFFHTIGSNRTITRGEPWTDRYIFPN
                     GVLPSISQLGRAMEELFVVEDWHNFGPYYDPTLMAWSANFEKAWPSLQPRYGDVFYRM
                     WRYYLLTSAGGFRSRHQQLWQVVFTRQGNPQPACRLS"
     gene            1174..2124
                     /locus_tag="PLW25_07295"
     CDS             1174..2124
                     /locus_tag="PLW25_07295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOS54326.1"
                     /translation="MLRTEVLIVGGGPAGSACAWQLKQHGVEFILLDRAEFPRPKVCA
                     GWVTPRVLRDIQLDPAAYPGNFSVFNQFLIELKGLRFTLQTRQYAIRRVEFDQWLINR
                     VRAQVITHQVKEIRQEGEYYLVDEEYRARYLIGAGGTNCPVKRALFEPEGQPVRGSLI
                     VAQEEEFVYPGASRLCHLWFLQQGLPGYAWYFPKSENVVNVGVGGSAARLKERGDTLR
                     HHWELLTERLYAYGLVRDHPFNPLGHSYYLRAAQPLLRKGNALLIGDALGVSTLDMGE
                     GIGPSIQSGINAATAIINHTPYTLDGISRYSFPSLLRLQR"
     gene            2199..2462
                     /locus_tag="PLW25_07300"
     CDS             2199..2462
                     /locus_tag="PLW25_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54327.1"
                     /translation="MARVIPIAQRISKARALMQKARDLPLPAEQGWLDFSYAANVRDI
                     LRQARDLLKFIQFMPNASAEAKREVEDLLLEINRAEKEILHKS"
     gene            2481..3173
                     /locus_tag="PLW25_07305"
     CDS             2481..3173
                     /locus_tag="PLW25_07305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOS54328.1"
                     /translation="MSDKTSRSKAVRQKSKKRSREYSDLTLILIVLLVLAVAIVGGYV
                     IKSRQAARVSPTAAVMNYKQYSAAPPMTIDPAKQYLATVKMAKGGEFVIQLYPDKAPV
                     TVNSFVFLAREGYFDGVTFHRVLAGFMAQTGDPSGTGSGGPGYQFENEDSDLVFDKAG
                     VVAMANAGRDTNGSQFFITYAPVEHLNGDYTIFGQVISGMEVVKDITRRDPQTNPKFS
                     GDVIESITITEQ"
     gene            complement(3201..3410)
                     /locus_tag="PLW25_07310"
     CDS             complement(3201..3410)
                     /locus_tag="PLW25_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOS54329.1"
                     /translation="MKRVELRVMDMHCTSCALRLQGLEDLLAGVIRVEASYRKGKMTV
                     TFDESKVTVKDLVEGARKLGYTAVE"
     gene            complement(3424..4461)
                     /locus_tag="PLW25_07315"
     CDS             complement(3424..4461)
                     /locus_tag="PLW25_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOS54330.1"
                     /translation="MAALIAALITGFTTGGLSCMAVQGGLLASSLAQLIEKDLLGKRG
                     GRDQWNIGLPIFLFLISKLAAYTILGGLLGGLGKLFQLDPLSQAVLQFIIAFFIIGNA
                     LRMLNVHPLFRYFTFEPPAFITRLIRKKTRQAASFTTPIMLGAMTVLIPCGVTQTMMA
                     AAIATGDVLSGAALMFAFTLGASPVFFLLSYFATKLGALTEKYFTRIAAVVLIVFGLL
                     AVNSGLNLLGSRYSLSRLAENAVSAVEIRHQGQASATPGTDLVLNVVDRGYRPKRLHA
                     PAGVPLTLTLVTKEVHSCSRAFLIPALDYGVILLATGEEKVQIPPQEPGTILPFTCSM
                     GMYSGEIIFDL"
     gene            complement(4654..>5347)
                     /locus_tag="PLW25_07320"
     CDS             complement(4654..>5347)
                     /locus_tag="PLW25_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54331.1"
                     /translation="LSTYQERKMDDLFVSAIVVVVTAVIVAAIFFITGKKKREKEAAL
                     LQLAQRKGWRYEKVNQGKHTGFILRAPEWTLESLVYSDYRTSEVGSSTVAYHNRWLTD
                     RVSSTHGLVMIGPKLPHAQLGALNSLMLRKALQLMLGDEADQAASLVEMQVGRSSFSE
                     RYSVWAVNQEGVEQVLTYNLENALLRWKGKELPVLKFSAAGAQISTRHGRLDSVEQVQ
                     EMVDLGMAVLAG"
BASE COUNT         1245 a         1501 c         1398 g         1203 t
ORIGIN      
        1 ctattgaata attcaggtgt ttttactaga ataacagtaa aggaggttat gatggcaagc
       61 gcagaatctg tcgttcgtga acttcttgac ctggcgcagg tcaaagtcaa cggtcccgac
      121 ccctgggata tccaggtgca caaccccgcg ttttacgatc gggtgctgcg cgacggcacc
      181 cttgggcttg gagaatccta tatggaaggc tggtgggatt gccctgccat tgaccagttc
      241 atcacccgcg ccttgctcgc caggctggat gaaaaggtta aaaacgaatc tcgttttttg
      301 ctgcaggcgc tcagatctcg ccttttcaac cgccagtcca gaggcagggc gtttgaagta
      361 ggcatcaagc attacgacct gggcaacgat ctctatcagg cgatgctgga taagcgcctg
      421 gtgtacacct gcggctactg gaaagacgct aaaaacctgg atgacgccca ggaagccaaa
      481 ctggaccttg tatgccgcaa aattggcgct catcccggta tgcgcattct tgagctcggc
      541 tgcggctggg gctcctttgc gaaattcgcc gctgaaaaat acggcgtcga ggtgctgggc
      601 gtgaccgtat ccaaagaaca ggtggcgctg ggcatggaat tgtgcaaggg gctgccggtg
      661 gagctgcgcc tgcaagatta ccgcgaggtc agcggtacat acgacgcggt catttccatc
      721 ggcgtgatgg agcatgtggg ttacaagaat taccgcgatt atatgctggt ggttgaccgc
      781 tgcctgaaac cagaaggtat cggcttcttt cacactatcg gcagcaaccg cacgatcacc
      841 cgtggtgaac catggaccga ccgctacata tttcccaatg gcgtgctgcc ttccatcagc
      901 cagcttggca gggcaatgga agagttgttc gtggtcgaag actggcataa ttttgggccg
      961 tattatgacc cgaccctgat ggcctggagc gccaattttg aaaaagcctg gccgtccctc
     1021 caacccaggt atggcgatgt tttctaccgt atgtggcgtt attacctgct caccagcgcc
     1081 gggggttttc gttctcggca ccagcagtta tggcaggtgg tcttcacccg ccagggcaac
     1141 ccgcagccag cctgtcgttt gagctgagag acaatgctgc gcacagaagt tctcattgtc
     1201 ggcggcggac cggctggttc tgcctgcgcc tggcaactca agcagcacgg ggttgaattc
     1261 atcctgctcg accgcgcaga atttccacgc cccaaggtct gcgccggctg ggtcaccccg
     1321 cgcgtgctgc gcgacatcca gcttgacccc gccgcctacc ccggcaactt ctcagtattc
     1381 aaccagttcc taattgagct gaagggcctg cgtttcacct tgcaaacgcg gcagtacgcc
     1441 attcgccgtg tggaatttga ccaatggttg atcaaccgcg tgcgcgcaca agtcatcacc
     1501 caccaggtga aagagatacg gcaggaaggc gagtattatc ttgtagacga ggaataccgc
     1561 gctcgttacc tcatcggcgc aggcggaacg aattgcccgg tgaaacgtgc tcttttcgag
     1621 cctgaaggtc agccagtccg cggcagtctg atcgttgccc aggaagagga atttgtttat
     1681 ccaggcgcca gccgcctgtg ccacctctgg tttttacaac aaggtttacc tggctacgcc
     1741 tggtatttcc caaaatcgga aaacgtggtg aacgtgggcg taggcgggtc ggccgcgagg
     1801 ttaaaggagc gcggagatac cctccggcac cattgggaac tgctcaccga gaggctgtat
     1861 gcatatgggt tggtccgcga tcatcccttt aatccgctcg gtcattcgta ttacctgaga
     1921 gccgcgcagc cactactcag aaaaggaaac gccttgttga ttggcgacgc cttgggggtt
     1981 tccacgctgg acatgggaga agggattggc ccatccatcc agagcggtat aaacgctgcc
     2041 acggcgatca tcaaccacac cccctacacg ctggatggca tttcccgcta ctcgttccct
     2101 tcgcttctgc ggctccaacg ctgaacgggc tgttcatttg tctcccaaaa ttctgcagtc
     2161 tatgctataa ctgtccaggc aacatctgcg gaatccatat ggccagggtg atcccaatcg
     2221 cgcaacgtat cagtaaggcg cgcgcgctca tgcaaaaagc gcgtgacctg ccgcttccag
     2281 ctgaacaggg ctggctggat ttttcctacg ccgccaatgt cagggatatc ttgcgccagg
     2341 cgcgcgatct tctcaaattt atccagttca tgcctaacgc ttcagctgaa gccaagcgcg
     2401 aggtggaaga tcttcttctt gaaatcaacc gcgcggaaaa agaaattctg cacaaatcct
     2461 gaatcataag gcgaaaaata atgagcgata aaacctccag aagtaaagcc gtcaggcaaa
     2521 aaagcaagaa acgcagccgt gagtattccg acctgacgtt gatcctcatc gttttactgg
     2581 tgctggcagt ggccattgta ggcggatatg tgatcaaatc tcgccaggct gccagggttt
     2641 cacctaccgc tgctgtgatg aactacaaac aatactcggc agcgccgccg atgaccatcg
     2701 accccgccaa acaatacctg gcgacggtga aaatggcgaa aggcggtgag ttcgtcatcc
     2761 agctttaccc cgacaaagcc ccggtaacgg tcaacagttt tgtgttttta gcccgtgaag
     2821 gctactttga cggtgtcacc ttccaccgcg tgctcgccgg ctttatggcg caaaccggcg
     2881 acccctcggg caccggcagc ggcggcccgg gataccagtt tgaaaatgaa gacagcgacc
     2941 tggtgtttga taaggcgggt gtggtggcta tggcaaacgc cgggcgagat accaatggca
     3001 gccagttttt tatcacctat gccccggttg agcacctgaa cggcgattac accatttttg
     3061 ggcaggtcat cagcggcatg gaagtggtaa aggacatcac caggcgcgac ccgcaaacca
     3121 atcccaaatt cagcggtgat gtgattgaaa gcatcactat cactgagcaa taacttgcca
     3181 gacagagagc tgtcatttaa ttattccacc gcggtgtaac cgagttttcg cgcgccttcg
     3241 accagatctt tcactgtgac cttgctttca tcaaaggtca cggtcatttt acccttacgg
     3301 taagaagcct ccacgcggat aacacccgcg agaaggtcct cgagtccttg caggcgcagc
     3361 gcgcagcttg tgcaatgcat atccataacc cgcaactcaa ctcgtttcat tttatccctc
     3421 tggttacagg tcaaaaatga tttcgccgct gtacatcccc atggaacagg tgaagggtaa
     3481 aatggtgcct ggttcctgcg gcggaatctg cactttctcc tcgccggtgg cgagtaaaat
     3541 cacgccataa tccagtgccg ggatcaagaa tgcgcgtgaa caggaatgga cctctttggt
     3601 cacaagggtt aaagttagcg gaacacccgc cggcgcgtgc agtcgctttg gacggtaacc
     3661 tctatcgacc acattcaaga caaggtcagt gcccggtgtt gccgacgcct gcccttgatg
     3721 gcgtatctcc actgcactga cagcattctc cgccagcctg cttaacgagt agcgcgaacc
     3781 cagcaggttt aaaccactgt tcactgccag taagccgaaa acaatcaaca cgaccgcggc
     3841 aatacgggtg aaatatttct cggtaagtgc gcccagtttg gtagcgaaat aagacaacag
     3901 gaaaaacacc gggcttgcac caagtgtgaa ggcaaacatc agcgccgctc cgctcagcac
     3961 atcccccgtg gcaatcgccg ccgccatcat cgtttgcgtt accccgcagg ggatcagcac
     4021 agtcatcgct cccagcatga tgggcgtggt gaaactggct gcctggcggg ttttcttgcg
     4081 gatgagcctg gtgatgaacg cgggcggttc aaaggtgaaa tagcggaata acgggtggac
     4141 gttcaacatg cgcaacgcgt taccgataat gaagaaggcg atgataaatt gaagtaccgc
     4201 ctgcgaaagt ggatctaact ggaataactt accaagcccg cccagcaacc ctccaagaat
     4261 agtgtaagcg gcgagtttac tgataaggaa aagaaaaatg ggcaggccga tattccactg
     4321 atccctgccg ccccgttttc ccagcaggtc tttttcgatc agctgcgcca gtgagctggc
     4381 caacaaacca ccctgcaccg ccatgcagct aagtccgcct gtcgtaaacc cggtgatcaa
     4441 cgccgcgatc aaagccgcca taagttaccc agagctcaat tcatataaaa agatgccatc
     4501 tctaatttcg agatattata tccatttaat ccgaaattag acctgtcttc ttttgggctt
     4561 ttttcaatgt caggcaaagg gataatccaa tgtaagtttc cctatccctg ggttaccggc
     4621 gggatactcc tgctcacctg catggatgat ctgctaacct gccagcaccg ccatgcccag
     4681 gtctaccatt tcttggacct gttccacgct atccaaccgt ccgtggcgcg tgctgatttg
     4741 agctccagcc gctgaaaact tgagtactgg cagttccttg cctttccagc gcagcaacgc
     4801 gttttccagg ttgtaagtta acacctgctc aacgccttcc tggttgacag cccacacgga
     4861 ataacgttcg ctgaacgagc tgcgtcccac ttgcatctct accaggctcg cggcctggtc
     4921 tgcttcgtct cccagcatca gttgcaaggc tttgcgcagc atcagactat tgagcgctcc
     4981 cagttgtgcg tggggtaatt taggcccaat catcaccagc ccgtgcgtgg aagatacacg
     5041 gtctgtcaac cagcggttat gatacgccac ggtcgacgaa cctacctcac tggttcgata
     5101 gtcagaatag accagcgact ccagcgtcca ttcaggtgcc cgcaggatga accccgtatg
     5161 tttaccctgg tttactttct catagcgcca acccttgcgc tgggccagtt gtaaaagagc
     5221 ggcttctttc tctcttttct tcttcccggt gatgaagaag atggcagcca cgatcaccgc
     5281 ggtgaccaca acgactatcg ctgaaacgaa aagatcatcc atttttctct cctggtaagt
     5341 tgataag
//
LOCUS       DAOSPM010000082         5091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_16552_length_5091_cov_13.375298, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000082 DAOSPM010000000
VERSION     DAOSPM010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16552_length_5091_cov_13.375298"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1466
                     /locus_tag="PLW25_07325"
     CDS             264..1466
                     /locus_tag="PLW25_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54332.1"
                     /translation="MKQVSMPIKTDSQVNIIVSDDLSLSGKNQLLMTALVKESDSLKF
                     SEDQGKVEIRATSDLSLQLPALQPVTVEKAGGDAHVSGLIQRVIIGKVGGDLALVDLA
                     GASVEMVGGDISLQRTTGAVEIARVGGDLVGSAVESVLSSAVGGDIYLRDVKGSLNLV
                     AGGDVVLQLASANPAMVTITAGGDVKIRVHAACNAQLELYTEGGSIRLNACGQQGEWD
                     QEQVVIPFGEGGNVVSIKAEGDILIIDHDDLDGVFQSRWDEMFENWEGFGSDLEKRVR
                     ESVAATSERIKWATLGTAAAGEKVRLKLEQAMRKLDERGKRLSERGITIDLPEKPGVC
                     GDINATGFRRGENDKVVGFTFPGENASTPTPTSKATDEERVMVLKMLQEKKITLEEAE
                     KILSALEK"
     gene            1479..2108
                     /locus_tag="PLW25_07330"
     CDS             1479..2108
                     /locus_tag="PLW25_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54333.1"
                     /translation="MTTDESKLEILRKVEDGTLTPEEGSELIGILDAAGAEESEREFI
                     RPPLEPETPGADQETLRVSGCWKAAWSMILLGGAVLTAFSADWIYLGYQKAGLGWSFW
                     LSWIPLAIGVLIMVLGWILMTSPWLQVRIRTHEKGRLQNIILNIPLPLSLVSWVFSNF
                     GQCMPDAVKEKGVEEMLLEIGQALKQNEPFQVEVDDQQDGDHVFICITR"
     gene            2176..2733
                     /locus_tag="PLW25_07335"
     CDS             2176..2733
                     /locus_tag="PLW25_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54334.1"
                     /translation="MKTAWRNAGLTLLCCSIFLFAFTFYKVRDTAVVSFTTVSGRSGT
                     VIVGLPRQVFIGDAVRLNAKVKVNSSSSEPMNLELAARLEAGFEELAPAGRVTLHLRS
                     GSEVGLIWKLRTGEEAVYPATLWIWVTSDAADELLLAREFTLQSRGFMGLAVRPVRIS
                     AGFLAASGLLVVGYGQTLRCRLKQY"
     gene            2805..3161
                     /locus_tag="PLW25_07340"
     CDS             2805..3161
                     /locus_tag="PLW25_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOS54335.1"
                     /translation="MLHEWLYLVIFLTISMFIPAAAIGAAALLSPKKPSPLKNSIYEC
                     GIETVGPSRIQFKAQYYLYGLLFLIFDVETVLLYPFAVAYNRLTLFAVLEAVLFVAIL
                     AAALVYAWRKGALEWT"
     gene            3184..4416
                     /gene="nuoH"
                     /locus_tag="PLW25_07345"
     CDS             3184..4416
                     /gene="nuoH"
                     /locus_tag="PLW25_07345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOS54336.1"
                     /translation="MSFFADPITFISNWLNSVLSNWGVPPGWAQFMLFILGAGALSMG
                     AMVFTIFLIWLERKLGGRFQDRIGPNRVGPWGIFQTIADMLKIFTKEYITPWGSDPVL
                     FNLAPVLAMAAVLAVWAVIPLSSTVYGSNIQIGVLYILAVGALGELGIILAGFASNNK
                     YALLGGFRVVAMLISYEVPMALALLVPVMLTGSMGLNDITSAQRVWFIWKAPVAALVF
                     FIASIAENSRAPFDLIEADSEIVAGFNIEYSGLKFGMFYVADFLHAFTAASIFASVFL
                     GGWRGPGAELYPALGFLYLLIKTAIVYFITISIRFSLPRLRVDQVMAFNWKYLTPLAL
                     VSLLVTALVDKSLPAGQGWLRLGGLLLANLVIFVVADRLVSIYHRRKNQERRVVSGPR
                     PIAVTLREKPAEPVEVES"
     gene            4413..4931
                     /locus_tag="PLW25_07350"
     CDS             4413..4931
                     /locus_tag="PLW25_07350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOS54337.1"
                     /translation="MTGMQIVFLINAAVTVIAGVMMVTVKRLMHAAMWLILTLFGVAV
                     VFGLLEASFFAIVQVVVYIGAIAILLVFAIMLTRDAVDDSFLQNHRWVLTALIGAVAL
                     SGLLLAIFTWPQSALFTAPLADAQRDVGEMGLSLLDPQGYLIPFEVSSILLLAALIGA
                     VHVGISRREEER"
     gene            4952..>5091
                     /locus_tag="PLW25_07355"
     CDS             4952..>5091
                     /locus_tag="PLW25_07355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HOS54338.1"
                     /translation="MLSAFLFSLGLFAVITRRNTIAILMGIELMLNAANINLLSFWRY
                     LHA"
BASE COUNT         1117 a         1170 c         1467 g         1337 t
ORIGIN      
        1 agcactattg cggtagatac ggtgatgttt ttcattgttt cctcgtttcc tggtccatat
       61 tggcgctagt gagtatacac ctattgccgc gcgctgatcg ataatggttt gagcaattca
      121 gttgcataat aagtgaaaat agaaataaat ataattaagg tattagagcg tataaacaaa
      181 agaatattaa taagagtatt gacaatacta aaattaattg atatactcat aaataaatga
      241 atgagaattg attgggaggg cgtatgaaac aagtatccat gccgattaaa acggatagcc
      301 aggtgaacat aatcgtttca gatgatctat cactttccgg caagaaccag ctactcatga
      361 cggctttggt aaaggaaagc gattcactca aattctctga agatcagggc aaggtggaga
      421 ttcgagcgac cagcgaccta tccttgcagc tgcctgcctt gcagccggta actgtggaaa
      481 aggccggcgg agacgcccat gtgagcggtt tgatccagag ggtgatcatt ggcaaggtgg
      541 gaggtgacct ggcgcttgtt gatctggctg gcgcctcggt tgaaatggtg gggggggata
      601 tttcgttgca acgaacaaca ggcgctgtag agattgcgcg ggttggcggt gatcttgttg
      661 gcagtgcggt ggaatcagta ctttcaagcg cagtgggcgg ggatatttat ctgcgggatg
      721 tgaagggcag cctgaacctg gtggcaggtg gagatgtagt gctgcaactt gcatcagcca
      781 atccagcaat ggtgacgata acagccggcg gagacgtgaa aatacgggta cacgcggcgt
      841 gtaatgctca actagaactt tacacagaag gaggttccat ccgcctgaat gcctgcggtc
      901 agcagggaga atgggatcag gaacaggtag tcatcccgtt tggcgaaggc ggcaatgtag
      961 tcagtattaa agccgaaggg gacatcttga tcattgatca tgatgacctg gatggcgttt
     1021 ttcagtccag atgggatgag atgtttgaga attgggaagg ctttggttca gacctggaaa
     1081 agcgggttcg cgagagcgtg gcagccacct cagaaagaat caaatgggca accctgggca
     1141 cggcggcggc tggtgaaaaa gttcgcctaa aactggagca agccatgcgc aaactggatg
     1201 agagaggaaa aaggctcagt gaacgcggaa tcacgataga tctgcctgaa aaacccggtg
     1261 tgtgtggcga tatcaacgcc acagggtttc gccgtggaga aaacgacaag gtggtgggtt
     1321 tcacctttcc aggcgaaaat gcttctaccc caactcccac cagcaaggct actgatgagg
     1381 aacgcgttat ggtattgaag atgttacaag agaaaaagat cacgcttgaa gaggctgaaa
     1441 aaatcctttc agcgctggaa aaataaggaa aattcgttat gactacggat gaatcaaaac
     1501 tggaaatttt gcgcaaggtg gaggacggga ccttaacacc ggaagaaggt tctgaactga
     1561 taggcatttt agatgccgct ggtgctgaag aaagcgaaag ggaattcatc cgaccgccgc
     1621 tggaaccaga gacccctgga gccgatcagg aaacgctgcg agtctcaggc tgctggaagg
     1681 ccgcctggag catgatcctt ttgggagggg cggttttgac tgcgttttcc gctgactgga
     1741 tttaccttgg ctatcagaaa gcagggttgg gctggagttt ctggctttcc tggataccct
     1801 tagccatcgg agtgttgatt atggtgttgg gctggatctt gatgacatct ccctggctgc
     1861 aggtgcgcat aagaacacat gagaagggca gacttcaaaa catcattctt aacatcccct
     1921 tgcctttaag cctggtctca tgggtattca gcaactttgg gcaatgtatg cctgacgcgg
     1981 tgaaagaaaa gggcgtggag gaaatgctgc ttgagatcgg gcaggcgctt aaacagaacg
     2041 agccgtttca ggtggaagtg gatgatcagc aggatggaga ccatgttttc atctgcatta
     2101 ccaggtgagt aagcagtttt cgttgaggat accggcgtga tttgccggta tttttttgcc
     2161 ggttataatc tttttatgaa gactgcatgg cgaaacgcgg gtttaaccct cctttgttgc
     2221 tcgatttttc ttttcgcttt tactttttac aaggtacgcg ataccgccgt tgtttccttt
     2281 acaacggttt caggacgctc cgggacggtc atagttggcc tgccgcgcca ggtattcatt
     2341 ggcgatgcgg tccggctgaa cgccaaagtg aaagtcaaca gttcttccag cgaacccatg
     2401 aaccttgagc tcgcggccag gctggaagcg ggatttgagg aattggctcc agctggacgg
     2461 gtgacactgc acttgcgaag cggctccgag gtggggctca tttggaagtt acgaacaggt
     2521 gaagaggctg tgtacccggc aaccttatgg atatgggtta cttctgacgc ggcggatgaa
     2581 ctcctgctgg cgcgggagtt tactctccaa tcgcgtgggt ttatgggact ggcggtcaga
     2641 ccggtcagga tttctgcggg attcctggcg gcgagcgggc tgctggtggt ggggtatggg
     2701 cagaccctcc gctgccgatt aaagcagtat tgagattggt tgcagcgaag ggcaaatatg
     2761 ataaaatttg cctgcattga atcacaaggt ttactgggag cactatgctg cacgagtggt
     2821 tgtatctggt catctttctg acgatatcca tgttcattcc ggcggcagcc attggggcag
     2881 ccgcgttgct gtctccaaaa aaaccttcgc ccctcaagaa ctcgatctat gaatgcggca
     2941 ttgaaacggt aggtcccagc cgcattcaat ttaaagctca gtattacctt tacgggttgc
     3001 ttttcttgat tttcgatgtt gaaactgtgc tcttatatcc ttttgccgtt gcctataacc
     3061 gcctgacgct ttttgccgta ctggaagccg tgctatttgt agccatcctg gcagccgccc
     3121 tggtttacgc ctggcgcaaa ggagcgctgg aatggacgta aggcagccgt cgggagacct
     3181 cggatgagtt ttttcgcgga ccccatcact ttcatcagta actggctgaa ttccgtcctg
     3241 agtaattggg gagttcctcc tgggtgggcg cagttcatgc ttttcatcct gggcgctggc
     3301 gctctcagta tgggggcgat ggtgttcacc atcttcctca tctggctgga acgtaaactt
     3361 ggcggacgtt tccaggaccg catcggaccc aaccgcgttg gcccttgggg aatcttccag
     3421 accatcgctg atatgctgaa gattttcacc aaggaatata tcaccccctg ggggtctgac
     3481 ccggtgttgt ttaacctggc gccggtgctg gccatggccg cggtgctggc ggtgtgggcg
     3541 gtcattccac tttcctccac ggtatacggt tctaacatcc aaatcggcgt gctttatatc
     3601 ctggcggtgg gcgcccttgg tgagctgggc atcattctcg ccgggtttgc ttccaataat
     3661 aagtacgcgc ttttaggcgg ctttcgagtg gtggcgatgc tcatcagtta tgaagtcccg
     3721 atggcgctcg ccttgctcgt gccagtcatg ttaaccggct cgatggggtt gaacgacatc
     3781 acctctgcgc aaagagtgtg gttcatctgg aaggcgccag tggcggcgct ggttttcttc
     3841 atcgcatcca ttgccgaaaa cagccgcgca ccttttgacc tgatcgaagc agattcagag
     3901 atcgtggcag gctttaatat cgaatactca ggcttgaagt tcggcatgtt ttacgtggcg
     3961 gattttctgc acgccttcac cgcggcgtcg atttttgcca gcgtcttcct gggtggctgg
     4021 cgcggacctg gcgctgaatt gtacccggca ctcggattcc tttacctgct cattaaaacg
     4081 gccatcgtgt atttcatcac catctccatc cgcttttcgc tgccgcgttt gcgagttgac
     4141 caggtgatgg cgtttaactg gaagtacctg accccgctgg cgctggtctc attgctggtg
     4201 acagcactgg tggataaatc cctacctgcc gggcagggtt ggttgcgcct gggaggtctg
     4261 ctattggcga acctggtaat cttcgtggtg gcggacagac tcgtttcgat ttaccacagg
     4321 cgaaaaaacc aggaacggcg ggtggtatct gggccgcgac ctatagctgt tacccttcgc
     4381 gaaaaacccg cagagcctgt agaggtagaa tcatgacggg gatgcagatt gtctttctca
     4441 tcaatgccgc cgtcaccgtc attgcaggcg tcatgatggt tactgtgaag cgcttgatgc
     4501 acgcagcgat gtggttgatt ctcactttgt ttggcgtggc ggtggtcttc ggtcttctgg
     4561 aagccagctt tttcgccatt gttcaggtgg tggtgtatat tggcgccatc gccattttac
     4621 tggtttttgc catcatgctc acacgtgatg ctgtggatga ctcttttctt caaaatcatc
     4681 gctgggtttt gacagcccta attggtgcag tcgcgctttc tggcttgctg ctggctattt
     4741 tcacctggcc gcaatccgct cttttcaccg ctccgctggc agatgcgcaa cgggatgttg
     4801 gagaaatggg gttgtcgctg cttgacccgc aaggttacct tattcccttc gaggtttctt
     4861 ctattctgct gctggcggct ttgatcggcg ccgtgcatgt gggcattagc cgtagagagg
     4921 aggagaggtg atcccgcttt actggttcct cgtgctatcg gcgtttcttt tctctttagg
     4981 gctttttgct gtcatcaccc gccgtaatac catcgccatt ttaatgggta ttgaattgat
     5041 gctgaacgcg gcgaacatca atcttttgtc tttttggcgc tacctgcacg c
//
LOCUS       DAOSPM010000083         4669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_18408_length_4669_cov_14.627005, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000083 DAOSPM010000000
VERSION     DAOSPM010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18408_length_4669_cov_14.627005"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PLW25_07360"
     CDS             <1..1203
                     /locus_tag="PLW25_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54339.1"
                     /translation="AMKETKVLDNKDIFCHELQLKMNQLAPVCAKMEPYEFRYCLNNI
                     TPEEGSWSSVLLETPETIEGWISSAEFFESIQIKPRVEGAIVLDATITRLTQMLFVGL
                     VTGEYATDWVHKWFYFDPRSFNFYARSFYLTDEIRHHLGGAPYRAFEQKQAAFELAHE
                     VGYQEFKAANEEIDRAYIETILALVEKKGTPIMLAIAGPTAAGKTEIVERLSARFAER
                     SQKIAAIELDNFLLDRDYREELNIGSFGARALHFDLLRSCVREILNKKTIFTPRYNFI
                     DGTSSHDLDGQRKPHGKPVQVDPADIVFIEGNSPFLLPGMAELVGIKCVYLTDDPVRL
                     KRKWRRDIDYRKKYDPYYLRNRFFKEQPPMAAKNYQPQLALCDIFVDTTHAALWVTPE
                     TRALLSPS"
     assembly_gap    1572..1671
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1672..2658
                     /locus_tag="PLW25_07365"
     CDS             <1672..2658
                     /locus_tag="PLW25_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOS54340.1"
                     /translation="HIDTVGVGDKETWPWDPFVGKVEEGVLYARGACDEKGSTPGMIY
                     GLSIARDLGLLKDYTVYYFGNMEEWCDGIAPNAFVDVDPKVRPDFVVIGEPTRMNVYR
                     GHKGRIELKVTASGRSAHAAVHYLGDNAVYKMISIINSIRELDRRFRFGMGSHPVLGH
                     ASVVVTDAAVRTNSLNAVPDQFTIFLDRRITLNESHDEVIDLIKGLIPDYLRDEIKVE
                     ELYYDTPSYTGFVFPLPKYFPAWLAEEKHPLVRAGQGTIEDLWGEPRPLGTWEFSTNG
                     TYWAGKAGIPSIGFGPGDEKDAHSPTEHVSLDDVVTATEFYAYLPYKLQQRA"
     gene            2850..>4669
                     /locus_tag="PLW25_07370"
     CDS             2850..>4669
                     /locus_tag="PLW25_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOS54341.1"
                     /translation="MSRKPSLKQRIQYHTDNLMARGPQAMIGLLGVFSLIIILLSAFV
                     VTIGGRIFFPEGVDNLSFIEAFWLSLVRTLDPGTMGADEGWGFRLIMLILPTLGGVFI
                     ISTLIGVLSNMIQNKIEDLRKGRSLVLEENHTIILGWSSQVFTILSELVEANLNQVNP
                     SIAILAMKEKVEMEDEIRSRLRDTKNTRIICRSGSPIDPAALELVRPQHARSIIVIPP
                     EDQDPDNFVIKTVLAITNNPHRRAEAYIIVTQIRDEKNLQVVQMLGKKDRVCALLARD
                     IIARVTAQTSRQSGLSIVYTELFNFSGDEIYFKEEKALTGKRYRDALTSYATSSVIGL
                     FKADATTVQLNPPMDSLIQPGDQLICISADDDTILYSASPDTPAIESIQSAPQPISVK
                     KERLLLLGWNDYGATILRELDNYVGYGSQITIVADETFQEKVSTCTRLKNQTIEFHAG
                     DTTNRSLLDELKVEDYDHVIVLADTSLDFQEADARTLVTLLHLRDISLQDETPFSIVS
                     EMLDLRNRELATVTKVDDFIVSTHLISLIIAQLSENIHLMPVFTDMLNEEGAEIYLKP
                     VEEYIKTGVEVNFYTVVEAAARRNETAIGYRSASAGEKSDS"
BASE COUNT         1143 a         1169 c         1197 g         1060 t
ORIGIN      
        1 gccatgaaag agaccaaagt gttagacaat aaggatattt tttgccatga gctgcagtta
       61 aagatgaacc agctggctcc agtttgcgcc aaaatggaac cttacgagtt ccgctactgc
      121 cttaacaaca tcacgccaga agaaggaagc tggtcatcgg tattgctgga aacgcctgaa
      181 accatcgagg ggtggatcag ctcggcggaa ttttttgaaa gtattcagat caaaccacgg
      241 gtcgaaggcg cgattgtact ggacgcgacc atcacgcgtt taacccaaat gctgttcgtt
      301 gggttggtga ccggtgaata cgcaaccgat tgggtgcata aatggtttta ttttgatcca
      361 cgcagtttta atttttacgc ccgttcgttc tatcttaccg atgaaatacg ccatcacctt
      421 ggcggtgctc cttaccgcgc cttcgagcag aagcaggctg cctttgaact ggcacacgag
      481 gttggctacc aggagtttaa ggcagccaac gaagagatag accgggctta catcgaaacc
      541 atactggcat tggtagaaaa aaaaggcacg ccaatcatgc tggcaattgc tggtccgacc
      601 gcggcgggaa aaacagagat cgttgagcgc ctgagcgcac gattcgcgga gcgatcgcaa
      661 aagatcgcgg ctattgagct ggataacttc ctgctggatc gtgattatcg cgaagagctc
      721 aatatcggct cttttggggc tcgcgcgctg cattttgatt tattgaggag ttgcgtcaga
      781 gaaattctca ataagaaaac cattttcacc ccgcgttaca actttatcga tggcacatcc
      841 agtcacgatc tggatggaca acgaaagccg catggcaaac cagtgcaggt tgaccccgcc
      901 gacattgtgt ttatcgaagg aaactcgcct ttcttgcttc caggcatggc tgaactggtg
      961 ggaattaaat gcgtatacct gacggatgac cccgtgcgtc tcaagcgtaa atggcgccgt
     1021 gacattgatt accgcaagaa atacgacccg tattatctgc gcaaccggtt tttcaaggaa
     1081 cagcccccca tggcggctaa aaattatcag ccccaactgg cgctgtgcga tatctttgtg
     1141 gatacaacgc acgctgctct gtgggtaacc cctgaaacca gagcattgtt atcgcccagc
     1201 tgaaaacctg ccgttccccg agtgtgaggt tgtgataaag aatggcgtct ttcgaggaac
     1261 cgtcgaaaca tgcccgtttg agcggctccc gctcaatgta tcggttccgt gaggtttact
     1321 cgcctgcgcc aagcacttga accgccaggt tagctagaac agacatcgac gattgggcgc
     1381 aaaaaaaaag aacttattga acacgaaacg cggcgtcacc gaagaaacca ggccttgatt
     1441 caagccagac cacaaaatgg aagcagcctg agcctgcgtg ttttaccagt tcttcgggga
     1501 ttcattgact attgtggcca taagtgtaca ataaaaggcg agccgggcag gcgcgctgaa
     1561 caggtcttca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacatcgac
     1681 accgtgggtg ttggcgacaa ggaaacctgg ccctgggacc cctttgtcgg caaggtggaa
     1741 gaaggcgtgc tttacgctcg tggcgcctgt gatgagaagg gcagcactcc cggcatgatc
     1801 tatggtttgt ctattgcccg cgacctgggc cttttaaagg attacacggt ctattatttt
     1861 ggcaacatgg aagagtggtg cgatggaatc gcccccaacg cctttgttga tgtcgaccca
     1921 aaggtacgcc ctgactttgt ggttatcggt gaacccacgc ggatgaacgt gtatcgcgga
     1981 cacaaagggc gcattgagct caaagtgacc gcttcaggcc gctcagcgca cgccgccgtg
     2041 cactacctcg gcgataatgc ggtttacaaa atgatttcca tcattaattc aatccgagag
     2101 ctcgaccggc gttttcgctt tggcatgggg tcacacccgg ttttgggtca cgcgtccgtc
     2161 gtcgtcacag acgcagcggt gcgcacgaat tcattgaacg cggtgcccga tcaattcacg
     2221 attttccttg accgacgcat cacattgaac gaatcgcatg atgaggtgat cgacctgatc
     2281 aaaggattga tcccagatta tttgcgggat gagatcaaag tggaagagct gtactacgac
     2341 acgccatctt ataccgggtt tgtgtttccg ctgcccaagt atttcccggc atggctcgcg
     2401 gaagaaaagc atccgctggt gcgagcaggt cagggaacaa ttgaagacct gtggggtgaa
     2461 ccccggccgc ttggcacctg ggaattttca accaacggga cttactgggc aggaaaagcc
     2521 gggattccct ccattggttt cggccccggt gatgaaaaag atgcgcattc tcccacggaa
     2581 cacgtatcgc tggacgatgt ggtaaccgcc accgagttct acgcgtacct gccgtataaa
     2641 cttcaacaac gtgcctgagc cagccttccc aacggataat caagcacgat tctcacctgc
     2701 ccgggttccc tttccgggca gtgtcttttc taccaggctc acctcgctat tcgaatgagg
     2761 agatgtttca atctgtagta tagtaaatga aagtcaaccg tttaccaggt catggccgcg
     2821 agacccgctg tggtaggaag gagattcaca tgtccagaaa accatccctg aagcaaagaa
     2881 ttcaatacca tacagataac ctgatggcgc gcggaccaca agccatgatc ggtttgttgg
     2941 gggtgttctc cctcatcatc attctcttat ccgcgttcgt cgttaccatt ggcgggagaa
     3001 tcttctttcc ggagggagtt gataatctat cgttcattga agccttctgg ttgagcctgg
     3061 tgcgaaccct tgacccgggc accatgggag cagacgaggg gtggggattc cgcctgatca
     3121 tgctgatcct ccctaccctt ggcggggtgt tcatcatcag caccttgatc ggcgtcctca
     3181 gtaacatgat ccagaacaag attgaggatt tgcgtaaagg tcgctcgctg gtattggaag
     3241 aaaaccacac catcatcctg ggctggtctt cacaggtgtt tacgattctg tcagagcttg
     3301 ttgaggcgaa tctgaaccag gtcaaccctt ctatcgccat tctggccatg aaagagaagg
     3361 tggagatgga ggatgaaatt cgttctcgcc tgcgcgacac caaaaacacg cgcatcattt
     3421 gtcgcagcgg ctcgcccatc gaccccgcgg cgctggagct ggttcgaccg cagcacgcgc
     3481 gttcgattat cgtgatcccc cctgaagacc aggaccccga taattttgtg ataaaaacgg
     3541 ttctggcaat caccaataac ccccatcgtc gcgcagaagc ctacattatt gttacccaga
     3601 tacgggatga aaaaaacctg caggtggttc aaatgctcgg aaaaaaagac cgggtatgcg
     3661 cgctgttggc gcgggacatt atcgccaggg tgaccgcgca gacttcccgt caatcaggtc
     3721 tatccattgt ctacaccgag ctgttcaatt tttcgggcga tgagatttac ttcaaggagg
     3781 aaaaagcgct tacaggtaaa cgctaccgcg atgcgctgac cagttacgcg acctcatcgg
     3841 tgatcgggtt attcaaagca gacgcaacaa ccgtccagct gaatccgcca atggactcgc
     3901 ttatccagcc gggtgaccaa ctaatttgta tctctgccga tgacgacact atcctttaca
     3961 gcgcgagccc tgatactcct gcaattgaga gcatccagtc agcacctcag cccatatcgg
     4021 tgaaaaagga gcgtcttttg ctgctgggct ggaatgatta tggagccacc atcctgcggg
     4081 aattggataa ttatgttggg tatgggtcgc agataaccat cgtggcggac gagacctttc
     4141 aagaaaaggt gagtacctgc acgcgcctga agaaccaaac gatcgaattc cacgcgggag
     4201 ataccacgaa ccgcagcctg ctggatgaac tgaaggtcga agactatgac catgtgatcg
     4261 tgctcgctga tacctcttta gattttcagg aggctgatgc gcgaacactg gtgacactgc
     4321 tccatctacg ggatatttct ttgcaagacg aaaccccatt ttcgattgtc agtgaaatgc
     4381 tggatctacg caaccgcgaa ctagccacag ttaccaaggt agatgacttc attgtgagca
     4441 cccacttgat ttcgttgata atcgctcagc tctctgaaaa catccatctg atgccggtgt
     4501 tcactgatat gctcaatgaa gagggggctg agatttactt aaagccggtt gaagagtaca
     4561 tcaaaaccgg ggtagaggtg aacttttaca cggtggtgga agccgcggcg cgaaggaatg
     4621 aaaccgctat cggctaccgc agtgcttcag ccggtgaaaa atccgattc
//
LOCUS       DAOSPM010000084         4372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_20079_length_4372_cov_12.781561, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000084 DAOSPM010000000
VERSION     DAOSPM010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20079_length_4372_cov_12.781561"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLW25_07375"
     CDS             <1..609
                     /locus_tag="PLW25_07375"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOS54342.1"
                     /translation="WLTWAQRIQALAQSGLYYTSDVYDAERFRELSSIACEIMEANTL
                     SQPGEVRMVFASQSGYATPKLDIRGVVFKEDKILLVRELQDQGKWTLPGGWVDINEPP
                     SQAVEREVREEAGKIVKAVKLLALYDSNQHGHPPLPFHSYKLFFLCEMLGETIPAPYE
                     TAEPSFFAQQEIPELSTTRVTHEEIQRLFEHLRDPSLPADFD"
     gene            667..2085
                     /locus_tag="PLW25_07380"
     CDS             667..2085
                     /locus_tag="PLW25_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOS54343.1"
                     /translation="MKKILVGVCKMVVVLVLSVTASCAPRSSPTPAITTTPIARITST
                     ANYSTSTPTIAAINPHVDIQKLDGLQIRFMHPWTGESLKLLFHMVDQFNQTNEWGIHV
                     IMQAPGSAARVSEKIHEELTNQIASNVLVAPVSLLLAIDERFQQVVDLSGYIASPLYG
                     MDPSLVNDFHQAFWDEGVVDGKLMGIPAQRSALLLAYNHTWGQELGFTSIPDTPAEFR
                     RQVCAANASFRKDADTANDGLGGWILNTDAVSMLNWLLVFGANPYSNGGFTFSTTSSQ
                     DALTYLYGLKTDACAWVNRSAADAERFSRRETLVYSLWLQELDKQVAAMERSGSKDEW
                     MLMTYPDGMKGQVLTQGSSYAVLHKDAESDLAAWLFIRWLSQPAQQFRLLTASGTLPL
                     GSQVMTQMEEYGAEHPHWLQIVNSLTRFTTPPVNADWQVIAPVLEDAGWQLWMGGTKE
                     DEITGLLAQMDDLAVELAERYP"
     gene            2085..2528
                     /gene="rnhA"
                     /locus_tag="PLW25_07385"
     CDS             2085..2528
                     /gene="rnhA"
                     /locus_tag="PLW25_07385"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOS54344.1"
                     /translation="MTDEIIIYTDGGCDPNPGLGGWAAVIIEGKNRREISGAAAFSTN
                     NRMELTAAIEALKVVKKNLPITLFTDSQYLKRGIDEWLPRWLAKNWRGANGPVANKDL
                     WQELLRVTRDRKVNWRWIYGHTGNPDNERVDFLCRLARSQFRRSY"
     gene            complement(2512..3984)
                     /locus_tag="PLW25_07390"
     CDS             complement(2512..3984)
                     /locus_tag="PLW25_07390"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOS54345.1"
                     /translation="MPESPEIILIIGAILILISVMVSKVSERLGIPLLLLFLLLGMVA
                     GSEGLGGIYFDDPLVAQWVCTVALSVILFSGGIDTEWKRIKPLFKEGLLLATLGVFLT
                     AAVVALFSRFILQLNWHVSLLIGAIASSTDAAAVFALLRSKGIRLKGNLAPLLELESG
                     SNDPMAIFLTIGILQWIQNKIDQPIEMILLFIMQMGVGLIVGWLMSRLSLFLINRLKL
                     GYEGLYPVLIFALVFLTFGLASLLGGSGYLAAYVLGLLLGKEDFLHKRSLVRFFDGNA
                     WLMQIALFITLGLLVFPSEIKPVILPGLLVSFVLIFLARPIAVFLTLIPFRYTLREKT
                     FISWVGLRGAVPVVLGTFPLVAGISDASAIFNLVFFIVITSVLLQGTLLPVVACWLKV
                     DAPLPVNSQAPIEVVSGQGLKADLREILITPSSPAVNKAIYQLKLPEEYLIIMVLRDG
                     EYIQPTGSLELKKGDILFSLSEEPVYLQAQNIINGVSTNA"
     gene            complement(4151..4222)
                     /locus_tag="PLW25_07395"
     tRNA            complement(4151..4222)
                     /locus_tag="PLW25_07395"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4187..4189),aa:Cys,seq:gca)
BASE COUNT         1126 a         1108 c         1181 g          957 t
ORIGIN      
        1 tggcttacct gggcgcagcg catccaggcg cttgcgcaga gcggactata ctacaccagc
       61 gacgtgtacg acgcggaacg cttccgcgag ttgagcagca tcgcctgtga aatcatggag
      121 gcgaacacac tctcacagcc tggtgaagtg cgcatggttt tcgcgagcca gagcgggtac
      181 gcgaccccca aactggatat ccgcggggtt gttttcaaag aggataagat tctgctcgtt
      241 cgtgaactgc aagaccaggg caaatggacg ctgccgggtg gttgggtgga catcaatgaa
      301 ccgccaagtc aggctgtgga gcgtgaagtg cgcgaagaag ccggaaagat cgttaaagcg
      361 gttaaactgc tagctctgta cgattccaac caacacggtc atccccctct tccgtttcat
      421 tcgtacaagt tgttcttcct gtgcgagatg ttaggcgaaa ccatccccgc tccgtatgag
      481 actgctgagc cgtctttttt tgcgcagcaa gaaatccctg aactttcaac aacccgggtc
      541 acgcatgaag agattcagcg tttgtttgag catttgcgcg acccttcgct gcctgccgat
      601 tttgattaac cgaaaattcg ggtatactct accttcgcaa cctgaggatt ggttgaagga
      661 aaagccttga agaaaatact cgttggcgta tgcaaaatgg tggtcgtgct ggtcttgagc
      721 gtgacggcgt cttgtgcacc gcgctcatct cccacccccg cgatcactac aacacccatc
      781 gcgcgcatta cctcaacagc caattacagc acaagcactc ctaccatcgc tgccatcaat
      841 ccccatgtgg atatccagaa actggatggt ttacaaattc gtttcatgca cccgtggaca
      901 ggggagtcac tcaaactgct tttccatatg gtcgaccagt tcaatcaaac gaatgagtgg
      961 ggcattcacg tgatcatgca ggctcctggt tcagcggcgc gagtgagcga aaagatccat
     1021 gaagaactca ccaaccagat cgcatccaac gtgctggtgg caccggtcag cctgttgctc
     1081 gccattgatg aacgctttca acaggtggtc gacctgtcag gctatatcgc ttctcccctg
     1141 tatggaatgg atccttcgct ggtcaatgat tttcaccagg cattttggga tgagggggtt
     1201 gtggatggga agctgatggg aatacccgcg cagcgcagtg cccttttatt ggcatataac
     1261 cacacctggg gtcaggagct tggctttact tccatccctg atacgccagc ggagttccgc
     1321 aggcaggttt gcgcggcgaa cgcttcattt agaaaagacg ctgatactgc caatgacggg
     1381 cttggcggat ggatactcaa tactgacgcg gtttccatgc tcaactggct gctggttttt
     1441 ggtgcgaacc cttacagtaa cggtggattc accttttcta caacctcatc gcaggatgcg
     1501 ctgacgtatc tttacggact caaaaccgat gcgtgcgcct gggtgaaccg ttctgccgcc
     1561 gacgcggagc gtttttcaag gcgcgaaacc ctggtttatt cgctgtggct gcaggagttg
     1621 gacaagcagg tggcggccat ggagcgcagc ggttcaaaag atgaatggat gttaatgacc
     1681 tatcctgatg gtatgaaggg gcaagtatta acccagggtt cttcgtacgc cgtgctgcat
     1741 aaagacgctg aaagcgatct ggccgcctgg ctgttcatcc gttggctgtc gcagcccgcg
     1801 caacaattca ggttgttaac cgcctcaggt acactgccgc tgggaagcca ggtgatgacg
     1861 cagatggaag aatacggtgc cgaacatccc cactggctgc agatagtgaa ctcgttgacg
     1921 cgatttacta cccctccggt gaacgctgac tggcaggtca tcgcaccggt gttggaggac
     1981 gccggctggc agttatggat gggcggcacc aaggaagacg agattactgg cttgctggca
     2041 caaatggatg accttgccgt agagcttgct gaaagatacc cctgatgaca gatgagatca
     2101 tcatttacac tgacggcggg tgcgatccga accctggttt ggggggctgg gctgcggtga
     2161 tcattgaggg caaaaaccgc cgggaaattt cgggtgctgc agctttttct accaacaacc
     2221 gtatggaatt gaccgctgcg attgaagcct taaaagtggt caaaaagaat cttccaatca
     2281 ctttgtttac tgattctcaa tatctcaaga gggggattga tgagtggcta ccgcgctggc
     2341 tggcaaaaaa ttggcgagga gccaacggcc cggtggcgaa caaagacctg tggcaggaac
     2401 tgctgcgcgt aacccgcgac cgaaaggtga actggcgctg gatatacggt cacacgggca
     2461 acccggacaa cgaaagagtt gactttttat gccggcttgc acgttctcag ttcaggcgtt
     2521 cgtactgact ccgttaatga tattttgtgc ctggagatag acaggctctt cagaaagact
     2581 gaagaggata tcccctttct tcaactccag gctaccggtt ggttggatgt attctccatc
     2641 tctcaagacc ataatgatga ggtattcttc gggcaacttt aactggtaaa tggctttatt
     2701 gaccgcggga gaggagggcg tgatgagaat ctcacgcagg tcggctttta atccctgccc
     2761 tgaaaccacc tcgatggggg cttgggaatt gacgggcagc ggcgcatcca ctttgagcca
     2821 acacgccacg acgggtaaca gggtgccctg cagcaagacc gaggtaatca cgatgaaaaa
     2881 aaccaggtta aagatggcgc tggcatctga aatgcctgca acgagtggaa aagtacccaa
     2941 cactaccggc accgctccgc gtaaacccac ccaggagata aaggtctttt ctctcaaggt
     3001 gtagcgaaaa ggaattaggg tcaggaaaac cgcgatgggg cgcgccagaa aaataagcac
     3061 aaacgaaacc agtaacccgg ggagaatgac cggcttgatc tcagagggga acaccaacaa
     3121 ccccaaggtg ataaaaaggg ctatttgcat caaccaggcg tttccgtcaa agaaccgcac
     3181 cagtgaacgc ttgtgcagga aatcttcttt gcctaatagc agccccaaca cataagctgc
     3241 caggtaaccg ctgcccccca gaaggctggc aagcccgaaa gtcagaaaca ccagcgcgaa
     3301 aatcaacacg gggtataagc cttcgtaccc cagcttaagc cggttaatca aaaagagcga
     3361 cagccggctc attaaccagc ccacaatcaa gccaactccc atctgcataa tgaacaacaa
     3421 gatcatttca atgggttggt cgatcttgtt ttgaatccat tgcaggatgc cgatggtcag
     3481 gaagatggcc atcgggtcat tactacccga ttcaagttca agcaggggag ccaggtttcc
     3541 tttcaggcgg atcccttttg aacgcagcag ggcaaaaact gccgccgcgt cagtggatga
     3601 ggcaatggcg ccgatgagca ggctcacatg ccagttcagt tggagaatga acctggagaa
     3661 aagagccacc acggcagcgg tgagaaacac ccccagcgta gccagcagca gcccttcttt
     3721 gaacaacggc ttgatgcgtt tccattctgt atcaatgccg cccgagaaga ggataacaga
     3781 gagggctacc gtacaaaccc attgagcgac caatggatcg tcaaagtaaa ttccgcccaa
     3841 tccctctgaa cccgcgacca tacccagcaa gaggaacaat aacaacaaag gaatccccag
     3901 ccgttcagaa accttgctga ccatcacgct gatcaggatg aggattgctc cgatgattaa
     3961 aataatttca gggctttcag gcatgtccca attatagtga atgaattctg ttttcgcaaa
     4021 attaatgaaa cttgaaagcg ctgcactgga gtaataacaa ctggggaatg gcggtttcag
     4081 gcgctctcaa tctaaggggg tgaagacgca tcgcaggtgc cttggtcgtt taacgaatta
     4141 atgaatgagg aggcgacgat gggatttgaa cccatgatca gggttttgca gacccttgcc
     4201 ttagcctctt ggccacgtcg cctcgcaatg aggattttat cagaaggtta aagggtggtc
     4261 aagcagcagg ctggatgtaa agaaaaaaga ggtaactttg gatgggcaat aggaatgagc
     4321 agcctcccag agcccaaaat aaaaacccct tttcaggggt ttgtctgagc gg
//
LOCUS       DAOSPM010000085         4237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_20951_length_4237_cov_14.195361, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000085 DAOSPM010000000
VERSION     DAOSPM010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20951_length_4237_cov_14.195361"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..700
                     /locus_tag="PLW25_07400"
     CDS             98..700
                     /locus_tag="PLW25_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOS54346.1"
                     /translation="MNLLLDPNVAYVILVIGFVLGVLALFTPGTGILEVGVLFAIVLA
                     GYAVIKLPINLWALILLMVGVVPFLIAVRKYKHWLYLIPAIASIVVGSIFLFKLESGA
                     PAIDPFLAVFMSVIAIVLLWFVARKGIEAMKLRPSQDLGALIGQVGVARSDIRTSGSI
                     YVGGEEWTARSDKPIREGQYARVIGRDGLVLVVEPAKEEK"
     gene            736..1641
                     /locus_tag="PLW25_07405"
     CDS             736..1641
                     /locus_tag="PLW25_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOS54347.1"
                     /translation="MFGEASILIYCLIGAVLILLVVFLAAAIKIVPEYRRIVVFRLGR
                     CIGAKGPGLILLIPFVDRPVTVDLREQVREIPAQQSITQDNAPLSIDFLWYYKVFDPV
                     QSVLQVQNFELAAQGIATTTLRSVIGGILLDGVLSEREKINTALRARLDEVTERWGVK
                     VTNVEIREIVPPRDVQDSMNRQLTAERNRRAVVTESTGSREAAINVAQGEKQANILRA
                     EGEKQATILKSEGERKAQVLKAEGYQQALEAIYAAARNIDQKTMTLQYFETLKTMASG
                     PSTKYIFPMEFTSLLSDFVKKNTEK"
     gene            1709..2176
                     /locus_tag="PLW25_07410"
     CDS             1709..2176
                     /locus_tag="PLW25_07410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS54348.1"
                     /translation="MTERPLFYELLPATLGDLSQLNALEKACFPLDAWPLIEQIAALT
                     FPATIRIKAEHKGRLIGFVGGDIRRAHQTGWITTLAVLPEYRRLGIGEALLRAGEQAL
                     RMPLVKLSVRRTNLEAQLLYLKNGYHKVETWRRYYEGGEDAIVMEKDMPAFKL"
     gene            2351..2599
                     /locus_tag="PLW25_07415"
     CDS             2351..2599
                     /locus_tag="PLW25_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54349.1"
                     /translation="MDYSLIGKIEKAKRYAQERERFEFHSFKVVVKGDNNAHVVDYEN
                     GAWKCDCEYFMSRGRCVHTMALESILEGMTLPEPVNAQ"
     assembly_gap    2856..2955
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2957..3044)
                     /locus_tag="PLW25_07420"
     tRNA            complement(2957..3044)
                     /locus_tag="PLW25_07420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3008..3010),aa:Ser,seq:cga)
     gene            complement(3071..>4237)
                     /locus_tag="PLW25_07425"
     CDS             complement(3071..>4237)
                     /locus_tag="PLW25_07425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS54350.1"
                     /translation="GYTGPIYATYPTATLGEIMLMDSAHIHEYDAEYINKKRARHGEP
                     PIEPLYNRADAARAIELYYPVHYNEPFSPVPGVTARFIDAGHILGSAAIRLDIEENGT
                     HKSLWFSGDIGRFGLPLITDPVMPSDVDYLLMECTYGDMEHFTVEEAYQKFSQAVVKT
                     IDRGGRVIVPAFAVGRTQEIVYFLNQMSTQGLISRLPVYVDSPLAVQASQIFASYPEY
                     FDEETNEFIRTQKHQALNFDGLTYISSVEESKALNDKKGPMVIISASGMAETGRILHH
                     LKNNIGNPRNTVLIVGWQAPDTLGRRLMEGADQLRIFGQLYDRRAEVQVITGLSAHAG
                     QHLLLDYAGAVKERVKEIFLVHGEEDAAGVFMGKLKEKGFAHFRYPSPGDTAEL"
BASE COUNT          975 a         1011 c         1188 g          963 t
ORIGIN      
        1 ctacagtaat catgctggac aattgggttt gcgatagaaa cctgtgaaat gagaaacata
       61 gtcgtgtata atgaattcag gaaaatgaaa ggatagtatg aacctgctgc ttgatccaaa
      121 tgtggcttat gtcatcctgg tgatcggatt cgttttaggc gtgctggcgt tgttcacacc
      181 cggcaccggt attttagagg tcggtgtgct gtttgccatc gtcctggccg ggtacgcggt
      241 catcaaactg cccatcaacc tgtgggcgct catcctgctg atggtgggcg tggtcccctt
      301 tctcatcgcc gtgcgcaagt ataagcactg gttgtactta atccccgcta tcgcttctat
      361 cgttgtcggt tcaatattcc tgtttaaatt ggaatccggc gctccggcga tcgacccatt
      421 cctggcggta tttatgagcg taatcgccat tgtgctgctc tggtttgtcg cgcgcaaggg
      481 catcgaagcc atgaaactgc gaccctcgca agacctgggc gcgctcatcg ggcaggtagg
      541 cgtcgcgcgc agtgacattc gcaccagcgg ctccatttat gtaggcgggg aggaatggac
      601 cgcgcgcagt gataaaccga tccgcgaagg acaatatgcc agagtcattg gtcgcgatgg
      661 attggtgttg gtagttgaac cggctaaaga agaaaagtaa tacattttcg ttttatctaa
      721 actgaaggag gagctatgtt cggagaagcg agtattctga tttactgttt gatcggggcg
      781 gtgctcattc tgttggtggt ctttttagcc gcggccatca agatcgttcc tgaatatcgc
      841 aggatcgtgg tcttcaggct ggggcgctgt attggcgcga aagggcccgg gttgatcttg
      901 cttattccct ttgttgatcg cccggtaacc gtagacctgc gcgagcaggt aagagagata
      961 ccggcgcagc aatccatcac gcaggataac gccccgttgt ccattgattt tctttggtat
     1021 tacaaagtgt ttgaccccgt gcaatctgtg ctccaggtgc agaattttga actggcggca
     1081 caaggtattg ccaccaccac gctgcgttcc gtcattggcg gtatcctgtt ggatggtgtg
     1141 ctgtctgaac gtgaaaagat caacaccgcc ctgcgcgccc gcctggacga ggtgaccgag
     1201 cgttgggggg tgaaagtgac caacgtggaa atccgtgaga tcgtgccgcc gcgtgacgtg
     1261 caggattcga tgaaccgcca gttgactgct gaacgaaacc gccgcgctgt ggtcaccgaa
     1321 tcaacgggta gccgcgaagc tgcgatcaac gtggcgcagg gtgaaaaaca ggccaatatc
     1381 ttgcgcgctg aaggtgaaaa acaggcgacg attctcaaat cagaaggcga acgcaaggcc
     1441 caggtgctga aggcggaagg gtaccagcag gcgctggaag cgatttacgc cgctgcgcga
     1501 aatattgacc agaagaccat gacgctgcag tactttgaaa ccctcaagac catggcgagc
     1561 gggccttcca ccaagtatat cttcccaatg gaatttacca gcctgttgag cgacttcgtc
     1621 aagaagaaca ccgagaagta aggacggatg attatgagta agttgtgggt tgggagagtt
     1681 tttcccaacc cacatttctt acgctgcgat gactgaaaga ccattgttct atgaactact
     1741 acccgcaacg ctgggagacc tttcccagtt aaacgcgctg gaaaaagcct gctttccact
     1801 ggacgcctgg ccgctgattg agcaaattgc tgcgctcacc tttccagcca cgatacgcat
     1861 caaagcggag cacaagggaa ggttgatcgg gttcgtcggt ggtgatatcc ggcgtgcgca
     1921 tcaaacaggg tggatcacca cgctggcggt gctgccggaa taccggcggc tgggtatcgg
     1981 agaagccttg ctgcgcgccg gcgagcaggc gcttagaatg ccgctggtga aactttctgt
     2041 gcgccgcacg aacctggaag ctcaattgct ttatttgaaa aacggttatc acaaagtgga
     2101 aacatggcga aggtattacg aaggcggcga agacgccata gtgatggaaa aagatatgcc
     2161 cgcgttcaaa ctgtagggca attgtcaggc aatcgtagtt gacaaagaac gtgaatgggt
     2221 ctaacataaa gtaagtggag aactgatcac catgcgagta ttgggttacc ataatggaga
     2281 aaacaactga aggcacgcct gttcaagcgt gcctttttgc taccatattg atgaaaagga
     2341 gctaatagaa atggattaca gtctgattgg caagatcgaa aaagcaaaac gctacgcgca
     2401 ggaacgcgaa cggtttgaat tccattcttt caaggtggtg gtcaagggtg acaataatgc
     2461 ccatgttgtg gattacgaga atggcgcctg gaaatgcgat tgtgaatact ttatgtcgcg
     2521 cgggcgctgt gtgcatacga tggcgcttga aagtatcctc gaagggatga cacttccgga
     2581 accggtgaac gcccaataac ctggtggaat acaatcaaga ccctggtgtc ttattgccag
     2641 ggtcttttta attgtcagag agggctgcgg tcttcgcgtc ctggaagccg ttctctggcg
     2701 gagagggccg cggagcgtcc tgtaaggatg aggattcgaa cccagattga cttagcgatg
     2761 agttattaag agcggagagg gccgcggagt gtcctgtaag gatgaggatt cgaacccaga
     2821 ttgacttagc aatgagttat taagagcgga gagggnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnngcgga gagggtggga ttcgaaccca cggtgaccac gagagccaca
     3001 acggttttcg agaccgcccc gatcaaccac tccggcacct ctccagcgcc agaattatac
     3061 ccgaaatctc tcacaactcg gcggtgtcgc ctggtgaggg gtaacggaaa tgcgcaaacc
     3121 ccttttcttt taacttcccc atgaacaccc cagcggcatc ctcttcaccg tgaaccaaaa
     3181 aaatctcttt taccctctct tttaccgcac cggcgtaatc caacagcagg tgctgaccgg
     3241 cgtgcgccga gagaccggtg atgacctgca cttcggcacg ccgatcgtaa agctgaccga
     3301 agatgcgcag ttgatcagcg ccttccatca ggcgcctgcc cagtgtgtct ggcgcctgcc
     3361 agccaacgat gaggactgta ttgcgcgggt tgccaatgtt gttctttaaa tgatgcagga
     3421 tgcgcccggt ttcggccata ccagaagccg agataatgac cattggccct tttttatcgt
     3481 tcagggcttt tgactcttct actgaactta tatacgtgag cccgtcgaaa ttgagcgcct
     3541 ggtgtttttg cgtgcggatg aattcgttgg tttcctcgtc gaagtattcc gggtaactgg
     3601 caaagatctg tgaagcctgc accgccaggg ggctgtctac atatacgggc agccgcgaga
     3661 tcaaaccctg cgtgctcatc tggttgagga agtaaacgat ctcctgtgtg cgcccgacgg
     3721 caaaagccgg gacaatcacc ctgccgccac ggtcaatggt ctttaccaca gcctggctga
     3781 atttttggta agcctcttcc acggtgaagt gttccatgtc gccataggtg cattccatca
     3841 acaggtaatc cacatcgctg ggcatgaccg gatcagtgat caggggcagc ccaaagcgcc
     3901 caatgtcacc tgaaaaccac aggctcttgt gggtaccgtt ttcttcgata tccaggcgga
     3961 tggcggctga accgaggata tgaccggcgt cgatgaacct ggcggttaca cccggcaccg
     4021 gtgaaaaggg ctcgttgtaa tgaaccgggt aatacagctc aatagccctg gcggcgtctg
     4081 cccggttgta gagaggttca atgggtggtt cgccatggcg ggcgcgtttt ttgttgatat
     4141 attcagcgtc atattcgtgg atgtgcgccg aatccatcaa catgatctcg ccgagggtgg
     4201 cggtggggta agtggcgtag atcggaccgg tataacc
//
LOCUS       DAOSPM010000086         3811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_24066_length_3811_cov_12.037540, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000086 DAOSPM010000000
VERSION     DAOSPM010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24066_length_3811_cov_12.037540"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1160)
                     /locus_tag="PLW25_07430"
     CDS             complement(105..1160)
                     /locus_tag="PLW25_07430"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOS54351.1"
                     /translation="MDLNEHQHFAQIDREDMLGHIMGLPLQLEKAWHLGKKQPLTGIQ
                     PVERIILAGMGGSAIGGDLLASYAADQLEVPLIVHRDYDLPACASGAGTLVVASSHSG
                     NTEEVLSAFERALRNKCQLIAMCTGGKLEALAREHHVPLWKFEHHGQPRAAVGYSFGL
                     LLALLTRLGLLPDPEREAQAAIKGMRALQEKIDAPIPVLKNPAKRLAGQLIGRHITVF
                     SSGFMSPVARRWKTQLNEVAKAFASFEYLPEADHNTLTGIWNPPGSETREFCLFLLAG
                     EDHPRNLLRLEKTKRILMLEGIGTDLFTAVGETRLEQMWNALLFGDYLAFYLAMIYDT
                     DPTPIPPIATLKEEMSA"
     gene            complement(1239..1418)
                     /locus_tag="PLW25_07435"
     CDS             complement(1239..1418)
                     /locus_tag="PLW25_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54352.1"
                     /translation="MSENFDPKVTTIAETDSYAVWSAEEPDGETTYNLELNNVTIHMF
                     EEEYAEFLELVKKLK"
     gene            complement(1411..2685)
                     /locus_tag="PLW25_07440"
     CDS             complement(1411..2685)
                     /locus_tag="PLW25_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOS54353.1"
                     /translation="MMTLQENPSSPGWSTNTKLVVGLALAGITILLLIRFHNILAPLL
                     TSVLVAYLFHPIATFFNKKLKIPWRLITTILFLLLFAIIIGLLTWGGISIVEQVQNLI
                     KLIQGMITDLPGFFEEISSKPLVIGPFEFDLSKLELETLWTQVAGIVQPLLAQLGSLI
                     GSLATGIGSTLTWLVFILLIAYFILSESNKANLIRIVIPRYQEDIQKIVTQLGHIWNA
                     FLRGQLLIFVITVAYYALLLSVLGVKNLFLLAFLAGLARFVPYVGPFVAWTTYGLVAL
                     FQTEHLGMQPLPYALLVVGCAWFSDFLLDNFVVPRVMSDALAIHPAAVLVMVIISASL
                     FGFLGILLAAPVLASIQLIVTYLISKLADQDPWLNLRVIQPPEKLSVIFIRQWQRTKN
                     AFLRINIFKRDTAKKQANNPQQSSKKKGEKNE"
     gene            complement(2759..3493)
                     /locus_tag="PLW25_07445"
     CDS             complement(2759..3493)
                     /locus_tag="PLW25_07445"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOS54354.1"
                     /translation="MANFRLILQSGSAAGTEYPLEKTEIYLGRDLSNDVVINDPEVSR
                     RHARLVLTGNTYVIEDLGSTNGTFLRGQRLSAPMLLNPGETITIGENVLLKFEGASID
                     PNATVAAFRRSSAEPVTSAAPAQQPFVPQQPAAPRYTPAQPAAPQYGAPQPAAPQFVP
                     QQPVTPPFATPQPAPPVHAAVGGASPAPYAPTAKKKSGWLIALLVVVGVLLIFCIVPW
                     LIIEVTDSYCALFPGIFNSIQPGVCP"
     gene            complement(3551..>3811)
                     /locus_tag="PLW25_07450"
     CDS             complement(3551..>3811)
                     /locus_tag="PLW25_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54355.1"
                     /translation="LLRQVQNYAAHGMVGSFFLNKPGSICIEKPPLVKRCLGRMSVSI
                     SRRCGSIEQPRLNVNTWAVFEIKPRSFSEMEVDERPRSNQNY"
BASE COUNT          958 a          955 c          962 g          936 t
ORIGIN      
        1 tgcgctttta ctcatgctga atttagtata gcaaatctgt tctaattgtc aagaaacaaa
       61 ctgcaggcaa ccgcgcctgc agtccgctgg tgttaatgat gggtttatgc gctcatttct
      121 tctttgagtg tggcgatagg aggaatgggt gtagggtccg tgtcgtaaat catcgccagg
      181 taaaacgcca ggtaatctcc aaagagcagc gcgttccaca tctgctcgag gcgtgtttct
      241 cctacggcgg taaagagatc cgtgccaatc ccttccagca tgaggatgcg tttggtcttc
      301 tccaaacgca gtaagttgcg cggatgatcc tcccctgcca gcagaaacag gcaaaattct
      361 ctcgtctcgc tgccgggtgg attccaaata ccggtcaggg tattatggtc agcctcaggt
      421 aaatactcaa aagacgcgaa cgcctttgcc acttcgttga gctgcgtttt ccagcgccgc
      481 gccacaggag acataaagcc gctggaaaac acagtaatgt gacgcccaat caattgcccc
      541 gccagccttt ttgctggatt tttcagcacc ggtattggcg cgtcaatttt ctcttgaagc
      601 gctctcatcc ctttaatggc cgcctgcgct tcccgctcag ggtctggcag caaaccaagc
      661 ctggttaata acgccaataa cagcccgaat gaatacccca ccgcggccct gggttgacca
      721 tgatgttcaa atttccataa cggaacatgg tgctcccgcg ccagcgcttc cagctttccg
      781 ccagtacaca tcgctatgag ctggcatttg tttcttaagg ctcgctcgaa agccgacagc
      841 acctcttccg tattaccgga atgagaagaa gccaccacca gcgttcccgc gcctgaagcg
      901 cacgccggca ggtcataatc gcggtgaacg attaaaggca cctctaactg atcagcggcg
      961 taagaagcca gcaggtcgcc gccaatggct gagccgccca tcccggccag aatgatgcgt
     1021 tccactggtt ggattccggt taatggctgt ttttttccga gatgccaggc tttctccagt
     1081 tgtagtggca aacccataat atgcccaagc atatcctcgc gatcaatttg ggcaaaatgc
     1141 tgatgttcgt ttaaatccat gcacaatcct tgattgtcgt ttttattgga gcgtccaatc
     1201 aatcatcaca acagagcaga tggtcgctct gttattcctt atttaagttt tttcaccaat
     1261 tccagaaact cggcgtactc ttcttcaaac atgtgaatcg taacgttgtt caattccagg
     1321 ttataagtcg tctcgccatc cggttcttcc gcagaccaaa ctgcgtaact atctgtttct
     1381 gcaatcgtgg tcactttcgg atcaaaattt tcactcattt ttttctcctt ttttcttact
     1441 tgattgttgt gggttatttg cctgtttttt agcagtgtct cttttaaaga tattgatcct
     1501 caagaaagca tttttcgtgc gctgccactg ccgtatgaag attaccgata gtttttccgg
     1561 gggttggatg accctcaggt tcagccaggg atcctggtct gcgagtttac ttatcaagta
     1621 ggtcacgatg agttgaattg aagccaaaac aggagccgct aacagaattc ccaggaaccc
     1681 gaacaggctg gctgaaatga tcaccattac gagtacagcg gcaggatgaa tggcgagcgc
     1741 atctgacatt actcttggca ctacaaaatt gtccagcaga aaatcagaaa accaggcgca
     1801 gcccaccacc agcagcgcgt atgggagtgg ctgcattccc aggtgctcgg tttgaaatag
     1861 cgccaccaag ccatatgtcg tccaggctac aaacggcccg acgtaaggga caaagcgcgc
     1921 gagccccgct aaaaacgcga gcaggaataa gttcttgacc cccagcacgc tgagcagaag
     1981 ggcgtaatag gcaacggtga tgacaaaaat gagtagctgc ccacgcagga aggcgttcca
     2041 gatatgccct agctgggtga cgatcttctg tatatcttcc tggtacctgg ggatgacaat
     2101 tctgatcaaa ttcgccttgt tgctctccga cagaataaaa tacgcaatca gaagaataaa
     2161 gaccagccag gtgagcgttg aaccaatacc agttgccagc gaaccaataa ggctacccag
     2221 ttgtgccaat aacggttgga cgatccccgc aacctgagtc cagagtgttt ccaattcgag
     2281 cttcgaaagg tcaaattcaa aaggtccaat gaccaaaggt ttactggaaa tttcctcgaa
     2341 aaatccaggc aggtcggtga tcattccctg gattagtttg atcaggtttt gtacctgttc
     2401 aacaatggaa ataccgcccc aggttaacag cccgataatg atggcgaaca agagcagaaa
     2461 aaggatggtt gtaatcaggc gccagggaat tttcagcttc ttattaaaaa aagtcgcgat
     2521 tggatggaat aaatacgcta ccaacacgga ggtgagcaac ggcgccagga tattgtgaaa
     2581 acggatgagc aagagaatgg taatgccagc caacgccagt cccaccacaa gttttgtgtt
     2641 ggtcgaccag cctggcgaag aggggttttc ctgaagtgtc atcatgatta ttataaggga
     2701 tagtggttaa aaaacgaaac tggctttacg cagccagttt cgtttggttt ttttgggttt
     2761 acgggcagac gcccggctgg attgaattaa agatccccgg aaaaagcgcg cagtaactat
     2821 ctgtgacctc aatgattagc caggggacaa tgcaaaagat aagcaaaaca cccaccacaa
     2881 ccagtaatgc gatcagccat ccagatttct ttttcgccgt cggcgcgtac ggagcggggg
     2941 aagcccctcc cacagcagcg tggacgggag gcgccggttg tggcgtggcg aagggagggg
     3001 tcacaggctg ttgcggcacg aactgcggag cggcgggttg aggtgcgccg tattggggcg
     3061 cagcgggctg ggcgggcgtg taacgcggcg ctgcaggctg ctgaggtacg aaaggttgtt
     3121 gggcaggtgc cgcggaggta actggctcgg cagaagagcg cctgaaagcc gccactgtgg
     3181 cgttaggatc gatggacgcg ccttcaaatt tgagcagaac attttctcct atggtgatgg
     3241 tttcacccgg attgagcaac atgggcgcgc ttaatctttg cccgcgcaaa aaagtgccgt
     3301 tggtagatcc caggtcctca atgacatacg tgttcccagt cagtaccaac cgggcgtgtc
     3361 ggcgagaaac ttcagggtca ttgatgacca catcattgct caagtctcgg ccaaggtaga
     3421 tctcggtctt ttcaagcggg tattccgtcc cggcagcact tccactttga aggattaaac
     3481 gaaaatttgc catatgctcc tccttatcat tttattagtg tagcaagtga atgtgagaat
     3541 gtcaacgatt ttaataattt tgatttgaac gtgggcgctc atcgacctcc atctcggaaa
     3601 acgatctcgg tttgatttca aacacagccc aggtgtttac atttagcctc ggttgctcaa
     3661 tggaaccaca ccgcctgctt atactcacgc tcatccttcc aaggcagcgc ttcaccagcg
     3721 gtggtttttc tatgcaaatt gatccgggtt tgttgagaaa aaaagacccc accatgccgt
     3781 gtgctgcgta gttttgaacc tgccgaagca g
//
LOCUS       DAOSPM010000087         3701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_24979_length_3701_cov_16.005760, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000087 DAOSPM010000000
VERSION     DAOSPM010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24979_length_3701_cov_16.005760"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /gene="rplL"
                     /locus_tag="PLW25_07455"
     CDS             complement(<1..239)
                     /gene="rplL"
                     /locus_tag="PLW25_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOS54356.1"
                     /translation="MADMEKLVEQLSGLTVLEAAELVKMLEEKWGVSAAAPVAVAAGP
                     AVAAAAEPEEEKTEFDVVLKDAGPKKIEVIKVIRAL"
     gene            complement(312..839)
                     /gene="rplJ"
                     /locus_tag="PLW25_07460"
     CDS             complement(312..839)
                     /gene="rplJ"
                     /locus_tag="PLW25_07460"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOS54357.1"
                     /translation="MAFTKKQKGEMTAQYEKWLEESKAMFVLEYVGLKMKEVDALRAK
                     AREAGSEVHLVKNTLFVRALQSGGYTVPEFFEGTSLVGFAFNDPPALAKVFADFIKGN
                     ETRKVKGGFLGKVIISETQVKALADLPPLPVMQATLLGVISAPATKLVRTLAEPARSM
                     AAVVKAYSEKAPVAA"
     gene            complement(1031..1750)
                     /gene="rplA"
                     /locus_tag="PLW25_07465"
     CDS             complement(1031..1750)
                     /gene="rplA"
                     /locus_tag="PLW25_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOS54358.1"
                     /translation="MAKHGKKYLEALTRVNKEQTYAPLEAVALAKETAYTKFDSTIEV
                     HLRMGLDPRQADQQVRDVCVLPHGLGKTVRVLVFAQGEAATKAQEAGADYVADSDEMI
                     NKIAAGWTDFDVAIATPEMMGKVGRLGRVLGPRSLMPNPKAGTVCLADDLPRVINEAK
                     AGRVEFRLDKTANIHVPIGKASFDTTKLYENFAALMEAIKKAKPAGAKGSYVKKVTVA
                     ATMGPGVKVDPLQAMNIALEK"
     gene            complement(1800..2228)
                     /gene="rplK"
                     /locus_tag="PLW25_07470"
     CDS             complement(1800..2228)
                     /gene="rplK"
                     /locus_tag="PLW25_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOS54359.1"
                     /translation="MSKKLKAIVRLQIQAGKANPAPPIGPALAGHGINIMAFCKEYNA
                     RTQNQVGEIIPAEITVFTDGSFTFILKSPPASELLKKAAGVPIGSKAPNREKVGKITR
                     AQLREIAEIKMKDMNALDVEGAMRQIEGTARNMGITIVEN"
     gene            complement(2300..2806)
                     /gene="nusG"
                     /locus_tag="PLW25_07475"
     CDS             complement(2300..2806)
                     /gene="nusG"
                     /locus_tag="PLW25_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOS54360.1"
                     /translation="MVHCYSGYENKVRYNLEQRIESMGMKDKIFDVVIPTQEEIEVRD
                     GKRRTVERHVFPGYVLVNMILTEESWYVVRNTPGVTGFVGMGNDPTPLRPEEVQQILR
                     RMEAEAPMVKVTFKIGERVRIVDGPFNDFRGIVSEIDMEKTKVRVMVNFFGRETPVEL
                     DFLQVEKA"
     gene            complement(3028..3240)
                     /gene="secE"
                     /locus_tag="PLW25_07480"
     CDS             complement(3028..3240)
                     /gene="secE"
                     /locus_tag="PLW25_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOS54361.1"
                     /translation="MADKNQKVKKENRFKRWWRETVGELRKVTWPTPREAWRLTKIVL
                     LVMVIMGAVLGGLDFMFTKLVGLVLG"
     gene            complement(3303..3378)
                     /locus_tag="PLW25_07485"
     tRNA            complement(3303..3378)
                     /locus_tag="PLW25_07485"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3343..3345),aa:Trp,seq:cca)
     gene            complement(3419..3568)
                     /gene="rpmG"
                     /locus_tag="PLW25_07490"
     CDS             complement(3419..3568)
                     /gene="rpmG"
                     /locus_tag="PLW25_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOS54362.1"
                     /translation="MRPVITFACDDCKERNYTSEKNRRNDPTRMELKKYCPRCKKHTL
                     HRETK"
BASE COUNT          780 a         1050 c          897 g          974 t
ORIGIN      
        1 agggcgcgga tgactttgat gacctcgatt ttcttcgggc cagcgtcctt gaggacaacg
       61 tcaaactcgg tcttttcctc ttcaggttca gcggcggcgg ccacagcggg tccggcggca
      121 acagccacag gcgcggcggc ggaaacgccc cacttctctt ccagcatttt taccagctcg
      181 gcagcttcca aaacggtcag accgctcaac tgctcaacaa gtttttccat atcagccatt
      241 tctattactc cttcgtttga ttgattcgtg tttatcgggt aattcattta cccggaggtt
      301 aagccactcg atcaggcagc gacaggcgct ttttctgagt aggctttgac gacagccgcc
      361 attgaacggg cgggttctgc cagcgtgcgt acaagcttgg ttgcgggtgc ggaaatcaca
      421 cccaacaatg tcgcctgcat cacaggcagc ggcgggagat ctgccagggc tttcacctgg
      481 gtttccgaaa taatgacttt ccccaggaaa ccgcctttca ctttgcgggt ctcgtttcct
      541 ttgataaaat cagcgaaaac tttcgccagc gccgggggat cattgaaagc aaaaccaacg
      601 agagaagtgc cttcgaaaaa ttccggaacg gtgtacccac cagattgcaa cgcgcgcaca
      661 aagagcgtgt tcttcaccag gtggacttca cttcctgctt cgcgggcttt ggcgcgcagc
      721 gcatccactt ccttcatttt taggccaaca tactccagca cgaacattgc cttgctttct
      781 tccagccatt tttcatattg ggcagtcatt tcgcccttct gcttctttgt aaaggccaat
      841 gaaaaatacc tccttccttt aaaaataaat cagtctttgt ccggttctgc cgcacaaaga
      901 ctgattccat tccccctcag gggagggtta tcgggattca atcttcacct cggcaggata
      961 ttgagtcttt gcagacaccg gctatctctg gtgaacgctg gaacgccttc cagcggagga
     1021 tattataaca ctatttttcg agtgcgatgt tcatcgcctg caacgggtcc accttaactc
     1081 caggacccat agtagcggcg acagtcactt tctttacata tgaacccttg gcgccagctg
     1141 gttttgcctt tttgatcgct tccatcagcg cggcgaagtt ttcatagagc ttcgtggtat
     1201 caaacgaagc cttaccaatg ggcacgtgga tattggcggt cttgtcaagt cggaattcaa
     1261 cgcggccggc tttggcctca ttgatcacgc gcggcaggtc atccgccaaa cagactgtgc
     1321 ccgccttggg gttcggcatc aatgaacgcg ggccgagcac gcgccccaaa cgacccacct
     1381 tacccatcat ttcaggggtg gcaatggcca catcaaaatc agtccaacca gcggcgatct
     1441 tattgatcat ctcgtcgcta tctgccacat aatccgcccc ggcttcctgg gctttggtgg
     1501 cggcttcgcc ctgagcaaag accagtacac gaactgtttt ccccagccca tgcggcagga
     1561 cacagacatc gcgaacctgc tggtcagcct ggcgcgggtc taaccccatg cgtaaatgaa
     1621 cttcaatggt ggaatcgaac ttggtataag ctgtttcttt tgccaaggca actgcttcaa
     1681 gtggagcgta ggtctgctct ttattgaccc tggtaagtgc ctccaggtat ttcttaccgt
     1741 gtttcgccat ttcttctcct tcgtggtgca aacgggtgtt tcaacaccct cccacctagt
     1801 taattctcta caatggtgat gcccatgttg cgggcggtgc cttcaatctg ccgcatggct
     1861 ccttcgacgt ccagcgcgtt catatctttc atcttaattt cagcgatctc gcgtaactgg
     1921 gcgcgggtga tcttgcctac cttctcgcgg ttgggcgcct ttgagccgat gggtacgcct
     1981 gccgcttttt tcagcagctc cgaagccggg ggtgatttta agatgaatgt gaatgaaccg
     2041 tctgtgaaaa cggtgatttc tgcggggatg atctcgccca cctgattctg cgtgcgggcg
     2101 ttatattcct tgcagaatgc cataatatta atcccgtgcc cggcgagggc gggaccaata
     2161 gggggtgcag ggttggcttt gccagcctga atctgcaaac gtacgattgc ttttaatttc
     2221 ttcgacactt tttttctcct tggtggttat aacgggtgat atttgggaca ccctcccaca
     2281 aacaggtcag cagacctggt tatgccttct ccacctgcaa aaaatccagc tccacagggg
     2341 tttcgcggcc aaaaaagttc accatcacgc gaaccttggt cttttccata tcgatctctg
     2401 aaacgatacc gcggaaatcg ttgaacggtc catccacaat acgcacgcgt tcaccaatct
     2461 tgaaggttac cttcaccatt ggcgcttcgg cttccatgcg gcgtagaatc tgctgcactt
     2521 cttcagggcg cagcggggtg ggatcgttgc ccatccccac gaagccagtg acgccaggcg
     2581 tgttgcgcac cacataccag gattcttcgg tcaagatcat attgacaagc acataacccg
     2641 ggaaaacatg gcgttccacc gtgcggcgtt tgccatctct tacttcaatt tcttcctggg
     2701 tggggatgac cacatcgaag atcttatcct tcatccccat cgactcgatg cgctgttcca
     2761 ggttgtagcg tactttattt tcataacctg aatagcagtg caccacaaac caggcgcgtt
     2821 cctgcgaacc atccgttgtg aggtcgcccg gtcgttcttc gtctttgacc tggctggaag
     2881 ggggtagttc ttcagggacg gcaggttgag atgcgtctaa ttcacccgaa gggcttgatt
     2941 cttcaggcag gttttcgtcc agctcatcct ggttgaaatc gtcctgcgtg tccataaaca
     3001 ccaaacctta atgtgcccag gatagggtta tcccagcacc aaaccgacaa gttttgtaaa
     3061 cataaaatcc aaaccgccca ggacagcgcc catgatgacc attaccagaa gcacgatctt
     3121 ggtcaggcgc caggcttcac gcggggtagg ccaggtcact ttgcgcagtt caccaaccgt
     3181 ctcgcgccac cagcgcttga aacgattttc tttcttgacc ttctgattct tatccgccat
     3241 gaccaactcc ttcttttcag ggtaaaacgc cgccatcacg acggcggtta cgtctcactt
     3301 tttggcaggc caggcaggaa tcgaacccgc aacctcctgt tttggagaca ggcactctgc
     3361 caattgagct actggcctgt acaggcaggg cggaaatccg ccctgccgaa ttacagcgct
     3421 atttcgtctc gcggtgcagc gtgtgttttt tacaccgcgg gcagtacttc ttcagttcca
     3481 tccgggtcgg atcgttacgg cgattctttt cggatgtgta gttgcgttct ttacaatcat
     3541 cgcacgcgaa cgtgattacc ggtctgatat ctttcttttt tgaagccata tttcactctt
     3601 gcagccgact ttgaccggct gacgctcctt ttatttcgat taagcgatga tttttgtgat
     3661 cctgccggcg ccaaccgtca gtccaccttc acgaatcgcg a
//
LOCUS       DAOSPM010000088         3486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_27063_length_3486_cov_13.109298, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000088 DAOSPM010000000
VERSION     DAOSPM010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27063_length_3486_cov_13.109298"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2801
                     /locus_tag="PLW25_07495"
     CDS             <1..2801
                     /locus_tag="PLW25_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS54363.1"
                     /translation="RFTAFKTDAIARKEIENHPPTDWPEQEEGEPPFICREHELELLN
                     QALRKRGVLLVQGEDGIGKTRLLKQFYITQPYRPALLYCRSQPLGQNTPFMALTQPLR
                     DQIQEVVWQALEPSDRQLLSHFYYHVLHGTEGYTLPVPAGEPQRVLENVFYAFLNLLK
                     ITASNRPLLFVLDDAMWMDQASISLISFLIEHKFFDQYGLLVILVSPEGDNPELDALL
                     QRRSRSRKLETIRLAPLQNREIGLFVQRIFGKTADEDLVKKMHCQTGGNPFYLIECLR
                     AMQQKNISSDQLSDFGACSPLESIVRLVRGKMNLLERASAAVLSAAAVLGRTFYIDVV
                     EEMTGVTDEALVAALNELSLKGFISANLEDRTEGSYVFSREVEREITLDQLGPAEKRR
                     LHLRAAKALLSRRPRQPAFAKEIALHFETAGETQPAVKAWLEAGRHARSIFSKDETYL
                     SYGRALELINKLPVGYDESVIYQVVNEWGNYAHDHDDSRTCEKIYQRCLEVGEATHSL
                     LLIGTGLSGLGRAADFLYEYEKSAEYFQRAIFYLSNTEYDAELIKALSRLGIMQFGMD
                     EYTRAYELLTDALKRDPATKDQDSLDNRVNILSYICFLHIFFGEPGKAEKIATEMARL
                     SVLVKRRSARVQGHALLAMAQYYNGRVQEALLTFRVNHSMAETLQVRFWLSLLELVAA
                     MAYLHNGDLDKSWYFIDRVHRREANYPQEKLFIQAVKIKGDIFRSLGANDKARGYYDQ
                     VFRSNVVNYPAVESRSLAAVMLAEERKNTDALELIQRSIEEAKQKNLAGIGLKARMYA
                     LLLFLEKTTFEEFEHQTGELQQEISRRELLNHDVFERWVPAALVELRGDQDRALAGYC
                     ELQQRMAASHNVWMEFLALRKIIGMTKRKDDAGSRARTRVKELLASIALHAESPAIKG
                     SFQKFRNKWRRYVNDMST"
     gene            complement(2913..>3486)
                     /locus_tag="PLW25_07500"
     CDS             complement(2913..>3486)
                     /locus_tag="PLW25_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOS54364.1"
                     /translation="IDTVVEKLEALAQKIDQKSRQKTTSPDLTAPPLRAGVRVKLRSL
                     KLEGVITSINEDDAEVMVGNLRVKLKTSDLAPVDEANPGIEKSKTAAPVPLPAPKPAA
                     TLFRPTPGMELDLRGMRAEDALDKLDAYLAEAYASGMPFVRIIHGKGTGRLRQVIREA
                     LRDSACVSTYEEGGEKEGGEGVTIAHLSED"
BASE COUNT          831 a          827 c          986 g          842 t
ORIGIN      
        1 cgcggttcac ggcttttaaa acggacgcga ttgccaggaa agagattgaa aaccaccccc
       61 caacggattg gcctgaacaa gaagagggtg agcctccttt catttgccgg gaacatgaac
      121 tggagctgct taaccaggcg ctgcgaaaac gcggtgttct gttggttcag ggcgaagatg
      181 gaatcgggaa gaccaggttg ttgaaacagt tttacattac ccagccgtac cgcccggctt
      241 tgctttattg ccgctctcaa cccctggggc aaaatacccc gtttatggca ttgacacagc
      301 ctttacgcga ccagattcag gaggttgtat ggcaggcgct cgagccttct gaccggcaat
      361 tgctttctca tttttattac catgtactcc atggaacaga ggggtacacg ctgcctgttc
      421 ccgctggaga gccacagcgc gtgctggaga atgtctttta tgcctttctc aacctattaa
      481 aaatcaccgc ctctaaccga ccgcttttgt ttgttctgga tgacgcgatg tggatggacc
      541 aggcgagtat ttccttgatt tctttcttga ttgagcacaa gttcttcgat caatacgggt
      601 tgctggtcat tctggtttct cctgaaggag acaacccgga attagacgca ttgttacagc
      661 gcaggagcag gtcgcgaaaa ctggaaacga tccgcctggc tccgctgcag aaccgtgaga
      721 ttggtctttt cgtgcagcgc attttcggta agacggctga tgaagacctg gtgaaaaaga
      781 tgcattgcca gacgggcggc aacccgtttt atttgatcga gtgtttgaga gcgatgcagc
      841 agaaaaatat atccagtgat cagctttctg attttggcgc ctgttctccg ctagagtcca
      901 tcgtcaggct ggtcagagga aagatgaacc tgctggaaag ggcttctgca gcggtgctgt
      961 cagcggcggc agttttaggg aggactttct acattgatgt cgttgaagag atgaccggcg
     1021 taactgacga agccctggtg gcggctttaa atgagttgag cctgaaaggc tttataagcg
     1081 ccaatcttga ggacaggacg gaggggagtt atgtgttcag ccgcgaggtg gaacgcgaaa
     1141 taaccctgga ccaattgggc cctgcagaaa agaggcgcct gcatctacgg gcggcgaaag
     1201 cgctgctcag ccgccgtccc aggcagcctg cgtttgcaaa agaaatcgcc cttcattttg
     1261 aaactgctgg agagacacag ccggctgtga aggcgtggtt ggaagcagga aggcacgcgc
     1321 gttcaatttt ttcaaaagac gaaacttatc tttcatacgg gcgggctttg gagttgatca
     1381 ataagttacc ggtaggatat gacgagtccg tcatttacca ggtggtgaat gagtggggaa
     1441 actatgccca cgaccacgat gattcgcgca cctgcgaaaa aatttatcag cgctgcctgg
     1501 aggttggaga agcaacgcac agcctgctgc ttattggtac cgggttaagc ggtttgggta
     1561 gagcggcaga tttcttatat gaatatgaaa aatcagcgga gtattttcag cgggccatct
     1621 tttacctgtc caacaccgag tacgacgcgg aactcatcaa agccttatct cgattgggga
     1681 taatgcagtt tgggatggat gaatacacac gtgcatatga actgctcacg gacgctttga
     1741 aacgcgatcc cgcgacgaaa gatcaggatt cacttgataa ccgcgtgaac atcctttctt
     1801 acatctgttt cctgcacatt ttctttggag agcctggcaa ggctgaaaaa atcgccaccg
     1861 agatggccag gcttagcgtg ctggtaaaaa gacgttcagc cagggtgcag ggtcacgcgc
     1921 tcctggcaat ggcgcaatat tataacgggc gcgttcaaga agccctgcta accttccgcg
     1981 tgaatcactc gatggcagag actctgcagg tacggttctg gctctcgctg ttggaactgg
     2041 tggcggcaat ggcgtacctg cacaatggag acctggataa gagctggtat tttatcgacc
     2101 gggtgcatcg cagagaagcc aattaccccc aggaaaagct ttttatccag gcggtcaaaa
     2161 tcaaaggaga tatcttccgt tcattggggg caaacgataa agcgcgcgga tattatgacc
     2221 aggtatttcg ttccaatgtc gttaattacc cggcggttga aagccgttcg ctggcggcgg
     2281 tcatgctggc ggaggagaga aaaaatacag atgcgttgga actgatacag cgttccatcg
     2341 aggaagcaaa acaaaaaaat ctcgccggga tcgggttaaa agcgcgtatg tatgcgctgt
     2401 tgttattcct ggaaaagaca acctttgagg aatttgagca tcaaacgggc gagttacagc
     2461 aggagatctc tcggcgcgaa ttgctgaatc atgacgtctt tgaaaggtgg gtaccggcgg
     2521 ctttagtaga attgcgtgga gatcaagacc gcgcgctggc gggttattgt gaactgcagc
     2581 aacgcatggc tgcgagccac aatgtttgga tggaatttct ggcgttgagg aagatcattg
     2641 gcatgaccaa aaggaaagat gacgcaggaa gtcgcgcgcg aacgagggtg aaagaactgc
     2701 tggctagcat cgctttacac gctgagtcac cggcgatcaa gggttctttt cagaaatttc
     2761 gtaacaaatg gaggcgttat gtcaacgata tgtcaacgta aaggttgtat aaatgtacaa
     2821 acattagcca ttatcgctgc agtcaaaggg agactgcgca catgaggccg gtagtgtttt
     2881 agggagaatc actatcggcc ttatccattc agtcaatctt cgcttaaatg ggcgatcgtc
     2941 acgccttctc cgccttcttt ttccccacct tcttcatacg tactgacaca tgcagaatcc
     3001 cgtaaggctt cgcggatgac ctggcgcagg cgcccggtgc ctttgccgtg gatgatgcgc
     3061 acaaagggca tgccactggc gtaagcctcc gccaggtagg catccagttt atccagcgca
     3121 tcttctgcgc gcataccacg caggtccagc tccatcccgg gcgttggtct gaaaagggtc
     3181 gcagcgggtt tgggagcggg caagggcacc ggcgcggcgg ttttgctttt ttcaatgcct
     3241 gggttggctt cgtccacggg ggccaggtcg ctcgttttga gcttcacgcg caggtttccc
     3301 accatcactt ctgcatcgtc ttcgtttatg gaggtaatca cgccttcaag tttcaacgag
     3361 cgcagtttta ctctgacccc ggcgcgcagc gggggggcag tcaggtcggg agaggtggtt
     3421 ttttggcgcg acttttgatc gatcttctgc gcgagcgctt cgagcttttc aaccaccgta
     3481 tcgatc
//
LOCUS       DAOSPM010000089         3371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_28259_length_3371_cov_13.191194, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000089 DAOSPM010000000
VERSION     DAOSPM010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28259_length_3371_cov_13.191194"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /gene="coaE"
                     /locus_tag="PLW25_07505"
     CDS             <1..1137
                     /gene="coaE"
                     /locus_tag="PLW25_07505"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOS54365.1"
                     /translation="RWPGKYVIGLTGSIGTGKSVVRRMLEHLGAYGIDADALAHRAMA
                     KGAPGYDPILRAFGRFLLTPEEQIDRARLGRLVFNDPDALAVLEHILHPLVLQAVDLL
                     VRRAPHKVVVIEAIKLFETDLVNMCDSLWVVYAPHEVQLSRLMQHRKMSEVEARQRIS
                     AQPPQETRFDRASVIIKNIASFEETWQQVTAAWQRLVPVKAVGAFPVSQPVKLPLGEV
                     NVQRAGPRQVEEIVRVYHQADAGNTAVDSNAIMAAFGDKAFLLLKIENRTMGVLGWQV
                     ENLVARTTEILLDPNVPAAQYLPILIREMEHASTQLQCEISLIYVPPHLARYDSLWIS
                     LGYAEKKPDDLNVLAWKEAALESMQLDSILFVKPLRQDRVLRPI"
     gene            1175..2056
                     /gene="cdaA"
                     /locus_tag="PLW25_07510"
     CDS             1175..2056
                     /gene="cdaA"
                     /locus_tag="PLW25_07510"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOS54366.1"
                     /translation="MIDLWSEIVYIFQRMNWLSVLDLFLVAAVVFAVLLLLRDTQIMV
                     LLRGVVVIILLLVLLTTFINLPAFSWLVTTIVPALLFAIPVIFAPEIRRGLEKLGRAG
                     SSQLLVRQTESQIQLMEKTIRSIVTAASRLSAREHGALIVMQRGVSLEEYARTGVRLN
                     ASVTPELLLQIFYPNTPLHDGAVIIADNKIVAGACVMPLSSSGILTRSPERQMGLRHR
                     AALGTSEATDAITVVVSEETGSISIAHNGRMIRRLDSERLENILLAFYQPVEHPVFKF
                     KPFDWFKKIVSVQKRED"
     gene            2056..3288
                     /locus_tag="PLW25_07515"
     CDS             2056..3288
                     /locus_tag="PLW25_07515"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOS54367.1"
                     /translation="MLARLRAFGKLIPLIIVSMVVAVAVWILAVTSSDPSETRLYAKP
                     VPIEVVGQSTNLVITNHLPESVTVNLRAPVSIWEKINSGKAGVRAFIDLSGVPEGTRT
                     TQVQVQVGIKPVEVISYSPQMVDLVLEKLSNKRFDIAVVSRGALPVGYQTEDPRLSET
                     SAVVSGAASLVAQVAEVRAVVDLSQVRSDINQEITLQPVDANGLLVREVSVTPDKITL
                     LQGVSQRGGYRNVVVKVVTEGLIKDGYRLKSVTVNPPTVTVYSSDTATVDALPGYVDT
                     RVINLVDRQEGFSVDVELSLPAGLQVIGSSMVEVKVEIEPVVSSLSLSEIQLEPTGLA
                     SNLSAVIVPNKANIILSGPVPLLNSLFVNDLRILIDLTGRLPGVYSIDVKPTLNIPGL
                     EIESISPATFEVTIRTKK"
BASE COUNT          696 a          881 c          994 g          800 t
ORIGIN      
        1 aggtggcccg gtaagtatgt gatcggtctc accggtagca tcggcaccgg caagagcgtg
       61 gtgcgccgta tgttggaaca cctcggcgcg tacggtattg acgctgacgc gttagcccac
      121 cgggctatgg cgaaaggcgc tccaggatac gaccccatcc tgcgagcctt tggacgtttc
      181 ctgctgaccc ctgaggaaca gatcgaccgc gcgcggcttg gccggttggt gttcaatgac
      241 cccgacgcgc tggcagtact tgaacatatc cttcacccgc tggtgttgca ggcggttgac
      301 ctgctggtaa ggcgggcgcc gcacaaggtg gtggtgattg aagccatcaa gttgtttgaa
      361 accgacctgg tgaacatgtg cgacagcctg tgggtggtgt acgctccgca cgaagtccaa
      421 ctctcacgcc tgatgcagca tcgcaagatg agcgaagtgg aagcccgtca gcgcatttcg
      481 gctcagccgc cgcaggaaac gcgttttgac cgcgcctccg tcatcattaa aaatattgct
      541 tctttcgaag aaacctggca gcaggtcacg gcagcctggc agcggcttgt accggtcaaa
      601 gcggtaggcg ctttcccggt gtcgcagccg gttaaactgc cgctgggaga ggtgaacgtt
      661 cagcgcgctg gaccgcggca ggtggaagag atcgtgcgcg tgtatcatca ggctgacgct
      721 ggcaatacgg cagtcgattc taacgcgatt atggctgctt ttggcgataa ggctttcttg
      781 ctgcttaaaa tcgaaaaccg caccatggga gtgcttggct ggcaggtcga gaacctggtc
      841 gcccgcacaa ctgaaatcct gctggacccg aatgtccctg ccgcccagta tttacccatc
      901 ctcatccgtg agatggagca cgcgtctacc caactgcaat gtgaaatatc gctcatctat
      961 gtaccacctc acctggcgcg ctatgattcc ctgtggatca gcctgggtta cgcggaaaag
     1021 aagcccgatg acctgaacgt gctcgcctgg aaggaagccg cccttgaatc catgcagctg
     1081 gattcgatct tgtttgtcaa gccgttgcgc caggaccgcg tgctgcgccc gatttaattg
     1141 ctttttacct gcgcgcaggt aaaattagga taatatgatt gatctgtgga gcgaaatcgt
     1201 ctacatcttc cagcgcatga actggctcag cgttctggac ctgttcctgg ttgccgcagt
     1261 cgttttcgcc gtgttgttat tactgcgtga cacgcagatc atggtgttgt tgcgcggggt
     1321 ggtggtaatc atcttgctgc tggtgctgct gaccaccttt attaacctcc ccgctttttc
     1381 ctggctggtc accactattg tgccggcttt actctttgcc atcccggtta ttttcgcgcc
     1441 ggagatcagg cgcgggcttg agaaacttgg gcgcgccggt tcgagccagt tgttggtacg
     1501 gcaaactgaa tcgcaaattc aactgatgga aaagaccatc cgctcgatcg tcacggcagc
     1561 ctcgcggctc tctgccaggg agcacggagc gctgattgtg atgcagcgcg gggtcagcct
     1621 ggaggaatat gcgcgcactg gtgtgcgcct gaatgcctca gtgacgcctg agctgttgct
     1681 gcaaattttt tatcctaata cgccgctgca tgatggcgcg gtcatcatcg cggataacaa
     1741 gattgtcgct ggtgcctgcg ttatgcctct ttcatcgagc ggtatcctca cgcgctcgcc
     1801 tgagcggcag atggggctgc gtcacagggc tgccctgggc acctcagagg ccacagacgc
     1861 aatcaccgtg gttgtgtctg aagaaacggg ttccatttcc attgcccata acgggcggat
     1921 gatccgccgt ttggacagcg aacggctcga gaatattctg ctggctttct atcaaccggt
     1981 tgaacacccg gtgtttaagt tcaaaccctt tgactggttc aagaagatag tcagtgtgca
     2041 gaagcgggag gattgatgct ggccagatta cgcgcgttcg gcaaattgat cccgctcatc
     2101 atcgtatcga tggtggtggc ggtagcggtt tggattctcg cggtcacttc ttctgaccct
     2161 tcagagaccc gtttgtatgc gaagccggtg ccgattgaag tggttggtca atcaacgaac
     2221 ctggtcatta ccaatcattt acccgaatcc gtcacagtga acctgcgcgc gccggtttct
     2281 atctgggaaa aaatcaattc aggaaaagcg ggcgtgcgcg ctttcatcga tctttcaggg
     2341 gtgccggaag gaacgcgcac cacgcaggtt caggtacagg tgggtatcaa gccggttgaa
     2401 gtaatttcat actctccgca gatggttgac ttggtactcg agaaactgag caacaagcgc
     2461 tttgatatcg cggtggtcag ccgcggcgcg ctgccggtgg ggtaccagac tgaggacccc
     2521 cggttgtcag aaacctcagc ggtggtaagc ggggctgcgt ctttggtcgc gcaggtggcg
     2581 gaggtgcggg cggtggttga cctgtcacag gtgcgttcag atatcaacca ggaaatcacc
     2641 ttgcaaccgg tggatgccaa tgggctattg gtgcgcgagg tcagtgtgac cccagataaa
     2701 atcaccttgc tgcagggggt atcgcagcga ggaggctatc gtaacgtggt ggttaaggtg
     2761 gttacagagg ggttgatcaa agatggctac cggttgaaga gcgtgactgt caacccgcct
     2821 actgtaacgg tttactcttc tgataccgcc acagtggacg cgctgcccgg ttatgtggat
     2881 acccgcgtca tcaacctcgt agaccgccag gagggcttca gcgtggatgt agaactgagc
     2941 ctgccggcgg ggcttcaagt cattggaagc tcaatggtag aagtgaaggt ggaaattgaa
     3001 ccggtggtga gcagtttgtc gttatcagaa attcaactgg aaccaaccgg gctggcttct
     3061 aacctgagcg cggtcatcgt gccgaacaaa gccaacatta ttctctccgg tccggtgccg
     3121 ttgctgaaca gcttgtttgt caacgattta cgcatcctga tcgatctgac cggtcgcctg
     3181 ccgggagttt actcaatcga tgtgaaaccc acgttgaata tccccgggct tgagattgag
     3241 agcatttctc ccgccacttt cgaagtgacc attcgtacaa aaaagtaacg tattccgcag
     3301 ggtggctctg gattaagggt acaataggtc tctgtatgag gactcatgtc taaacctgtt
     3361 gttgctctgg t
//
LOCUS       DAOSPM010000090         3232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_29837_length_3232_cov_16.084042, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000090 DAOSPM010000000
VERSION     DAOSPM010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29837_length_3232_cov_16.084042"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLW25_07520"
     CDS             <1..757
                     /locus_tag="PLW25_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54368.1"
                     /translation="PPAEAPTPPPGTELNLGGIYMLIPPCLPVSASDLTVPAQVYDPE
                     GGPQEVYPQHRRLSFSGYPLSGTFFEPFMRVFPVEGFTSAYAAAPENYAAPIIAQMQT
                     LLATRPPDGDASLPFLVMAGAGQVFHAQAKYLDFANGSGVRYLTSYGQYYVPYNNHDL
                     FYTFQGLTTDGKYWVSVIFPVNHPLLPPTYDSSLVPPGGIAIPSFTSPSYDEDMSAYY
                     AAMKALMNSQPDNTFTPALDCLDAYITSLHVGD"
     gene            1014..2468
                     /locus_tag="PLW25_07525"
     CDS             1014..2468
                     /locus_tag="PLW25_07525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOS54369.1"
                     /translation="MQLAYLNPLILFPVIGLIAFIYYILPRSTRWIFLLVASYAFYYS
                     VGSWTIIVLVAVTALNYFFGLGLGSGQFSKKRALLVCGIILNILFLFLFKYLSSLLAP
                     GSMLMTILASKTGRLLIPAGLSFYTLQNISYLVDVSKGIIKPERNPGIFALYLAFFPK
                     ILSGPIERGKKLLPQFHHPSALNWDDITAGMRLMLFGLFKKVVIADRLALFVNEVFNN
                     PGTYHSWTVMIALVFLSFQIYLDFSGYTDLALGIARVFGINLTQNFKRPYLSENIVEF
                     WNRWHLSFSTWLRDYIFYPLRRFFLKSGLKSTGFVSLGIPPVVTMALSGIWHGVGLTF
                     AVWGLYHAFFYTLVVWWRSTHTVNHQKPLVLKIFLVLVNFAVLTFSWLIFRSDNLQHA
                     FTLFNSLIVPGQMQYDLHEILLHTYVFDFQVSRVAILVVIAVEVFLEVKKDSFKFENA
                     PKWLRWSVYLLLILSLTTIGLYQGGDNPFVYFKF"
     gene            2524..>3232
                     /locus_tag="PLW25_07530"
     CDS             2524..>3232
                     /locus_tag="PLW25_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54370.1"
                     /translation="MNAVLLMNYRQNNLDSYVAAVFDKQALLSSTPSPKIILVGGSNV
                     AFGFDSRRLSEGTGLPVVNAGLQGGLGLHFMLNSIKPSINRGDIVLLSPEYHILFNRL
                     SWGEVLAQMLVIDPGSWKNISTFNEVWQLLKTFPTVHTEAIRNRFELEMNPKCQFCKS
                     DMSVYSREAFDPETGDITTNQLEGKPEEDFSMKLGYALPNFWIKKSIRSLNRFNEYVV
                     AKGATLFFVYPNTVTPTD"
BASE COUNT          688 a          911 c          749 g          884 t
ORIGIN      
        1 accgccggcc gaagcgccca ccccgccccc cggcaccgag ctcaacctcg gcggcatcta
       61 catgctcatc ccgccctgcc tgcctgtctc cgccagcgac ctcaccgtgc ccgcccaggt
      121 atacgacccc gaaggtgggc cgcaggaggt gtacccgcag caccgccgtt tgagcttcag
      181 cggctacccg ctcagcggca ccttctttga gccgttcatg cgcgtcttcc cggtggaggg
      241 gtttacctcc gcctacgccg ccgcgcctga aaactacgcc gcccccatca tcgcccaaat
      301 gcaaaccctg ctcgccaccc gcccgccgga tggcgacgcc tccctgccct tcctggtaat
      361 ggccggcgcc ggtcaggtct tccacgccca ggcgaagtac ctcgattttg ccaacggctc
      421 gggggtgcgc tacctcacct cttacgggca gtattacgtg ccctacaaca accacgacct
      481 cttctatacc ttccaggggc tcaccaccga cggcaagtac tgggtgtcgg tcatcttccc
      541 ggtcaaccac cccctgctgc cgcccacgta tgattccagc ctggtgcccc ccggcggcat
      601 cgccatcccc tcgttcacct cgccttcgta cgacgaggac atgagcgcct actacgccgc
      661 catgaaggcg ctgatgaaca gccagcccga taacaccttc acccccgcgc tggactgcct
      721 ggacgcctat atcacctccc tccacgtcgg ggattgagcg cggcgcgtat ttttgaggaa
      781 tgcgcccctg gcgtgtactt ggtacgccca gcatgaaaac tgctgggcgt ctttacctgc
      841 gccagggtgc gccagcctgc gcggatgagg cgcttccccc gcgttccagg ttgctcgtgc
      901 agcatccatc gtcatctcct taaataaaac cactggtgct tcataatagg atggttaatt
      961 cgctggaata caggtaaaat ggttcattgt taaattcaat gtaaactgta tatatgcagc
     1021 tggcttatct caatcccctt attctttttc ctgtaatcgg cctgatagcc ttcatatact
     1081 atatccttcc acgcagtacg cgctggatat ttctgctcgt cgccagttac gctttctatt
     1141 acagcgtggg atcgtggact atcatcgtct tggtcgctgt caccgcgctc aattactttt
     1201 ttggtcttgg cctgggatct gggcaatttt caaagaaacg ggccttgctg gtatgtggaa
     1261 ttatcctcaa tattctgttc ctgtttctgt tcaagtatct atccagcctc ctggctccag
     1321 gttcaatgct gatgactatc ctggcttcaa agaccgggag gcttctgata ccagccggct
     1381 tatcttttta cacgctgcaa aacatcagtt acctggtgga tgtatctaaa ggaataatca
     1441 aaccagagcg caacccaggg atttttgccc tctatcttgc tttttttccg aaaattctgt
     1501 cggggccaat cgagcgcgga aagaaattac tccctcaatt tcaccatcca tccgctttga
     1561 attgggatga tattaccgcc ggtatgcgct tgatgttgtt tggcttgttt aaaaaagtgg
     1621 tcattgccga caggctggcc ctttttgtga acgaagtctt taacaacccc gggacttatc
     1681 acagctggac cgttatgatt gcgctggttt ttctgagttt tcagatttac ctggatttct
     1741 ccggttatac cgatctcgcg ctgggtatcg ccagggtatt tggcatcaac ctgacccaaa
     1801 atttcaaacg cccttatctc tctgaaaaca tcgtggagtt ttggaatcgc tggcacctct
     1861 cgttttctac atggctgaga gattatatct tttaccccct gcgaagattt ttcttaaaat
     1921 cgggtctcaa gagtaccggg tttgtgtcct tgggtattcc tccggtggtc accatggctt
     1981 tgagcgggat atggcacggc gttggattaa cctttgccgt ctggggtttg taccacgcct
     2041 tcttctatac gttggtcgtg tggtggcgaa gcacccatac ggtaaatcac caaaagcctc
     2101 tggttttaaa gatatttctc gtattggtga actttgccgt gctgactttt agttggctga
     2161 tctttcgttc ggacaacttg cagcacgcgt ttacgctttt caatagccta atcgtacccg
     2221 ggcagatgca atatgacctg catgaaattt tacttcacac ctatgtgttc gattttcagg
     2281 tttcccgtgt tgcgatcctg gtggtcattg cagtagaagt atttctcgag gtcaaaaagg
     2341 attcgttcaa gtttgaaaac gctccaaaat ggcttcgctg gagtgtttat cttcttttga
     2401 ttctttcgtt aaccacaatc ggtttgtacc agggaggaga caaccccttt gtctacttca
     2461 aattctaggc gtctcctggt tttcaccatt aaccttctca gcattttcct gataacgctg
     2521 gtagtgaacg ctgtcctttt gatgaattac cgccagaaca acctcgatag ttacgtggca
     2581 gccgtttttg ataaacaagc cttgctttct tccaccccat cgcccaaaat catcctggtg
     2641 ggaggttcta acgtagcctt cggcttcgac agccggcgcc tgagtgaagg cacaggtctg
     2701 ccggtggtaa atgccggctt acagggcggg ctcgggttgc attttatgct caacagtata
     2761 aaaccctcta ttaatcgcgg ggatattgtg ctcttatccc ccgagtacca catccttttt
     2821 aaccgcctta gttggggtga ggtgctggcg caaatgctgg tgatcgaccc gggaagctgg
     2881 aaaaatattt caacattcaa tgaagtatgg caattgctta aaacatttcc aaccgttcat
     2941 acagaagcca tccgcaatag gtttgaactt gaaatgaacc ccaaatgtca attctgtaaa
     3001 agtgatatga gtgtctattc acgagaagcc ttcgaccctg aaacaggtga tatcaccacc
     3061 aaccagttgg aaggcaaacc tgaagaggat ttctccatga aactgggtta tgctttgccc
     3121 aatttctgga ttaagaaatc cattcgctca ctgaacaggt tcaatgaata tgtggtcgcg
     3181 aaaggcgcga cgctcttctt tgtctacccc aatacggtca caccaaccga tg
//
LOCUS       DAOSPM010000091         3035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_32390_length_3035_cov_13.952349, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000091 DAOSPM010000000
VERSION     DAOSPM010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32390_length_3035_cov_13.952349"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLW25_07535"
     CDS             complement(<1..323)
                     /locus_tag="PLW25_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOS54371.1"
                     /translation="MQLLLATTNKGKIGELSALLAGLPLQLLTPQEVSPDTRVEETGR
                     TYLENALLKAFAYQAATGLAVLADDTGLEVDALDGAPGLHSARFSPLPGATDADRRAL
                     LLAHLA"
     gene            436..915
                     /locus_tag="PLW25_07540"
     CDS             436..915
                     /locus_tag="PLW25_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOS54372.1"
                     /translation="MANQRNPLKINVGFFNNLPIGSFRDIHFESPDLHLPPDLDVTNF
                     SGSVHISRTPQGLLFDGAFRAFIQAECVRCLEPYDQLLETEFSEVYAYKSHSFTESNL
                     FVPDDGNIDLSPVIREYLMLENPIKPLCKPDCQGLCVVCGENLNIATCEHQARIKIE"
     gene            969..>1279
                     /locus_tag="PLW25_07545"
     CDS             969..>1279
                     /locus_tag="PLW25_07545"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HOS54373.1"
                     /translation="MANPGRRSKSAEVLNLLLEKADVQGYLTTEDLMEFYPDVTRDAE
                     RIEAVLTALRRRGVEILDGENDSDITDNEGYAQVTDLDFTSTFENISSDDTIGLYLKE
                     MS"
     assembly_gap    1280..1379
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1516..1902)
                     /locus_tag="PLW25_07550"
     CDS             complement(1516..1902)
                     /locus_tag="PLW25_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54374.1"
                     /translation="MIKKKETSAEVAVSPFLPTEEEEADVISELQQSLPPEDEEETGY
                     TASEVAEYTITQQINAILQEMLQTTELADKGIRLEENPNHSVDVFVGAEKYSGIEEVP
                     YPQVRSLIHEAVLRWEQETNPQQRIG"
     gene            complement(1874..2296)
                     /locus_tag="PLW25_07555"
     CDS             complement(1874..2296)
                     /locus_tag="PLW25_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54375.1"
                     /translation="MSAQGSTLLLLLAALIGLFIGLLLSSLSSGKEGKRKSQPPPEIL
                     KEGYGEVARLWYSPGTKKVLTEMDGGFYKSVNGLSGEQKAKLKRLNALLNEWVGAEPP
                     VETAPQATVVENTHYQPLPDPVSRENYDFYDKEEGDLR"
     gene            complement(2382..>3035)
                     /locus_tag="PLW25_07560"
     CDS             complement(2382..>3035)
                     /locus_tag="PLW25_07560"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOS54376.1"
                     /translation="KFLVFQLEKDWMLIHLRMSGDLRVEPRDKTTLGLHDRIIFNFAD
                     DARMVFNDTRKFGRAWLLKDPQQVLGTLGPEPFDPELTAGAFHSMLHKKKRAIKPLLM
                     DQKFLAGMGNIYTDEALHLARVHPLEKASSLNYSQAESLLRAIREVLTEGIRRNGASI
                     DWAYRGGDSRITSGCISAMARNAPCAATISNESWSASGGLIFVLLASCWKKAKKTLL"
BASE COUNT          715 a          772 c          713 g          735 t
ORIGIN      
        1 gccaggtgcg caagcagtaa cgcgcgtcgg tctgcatccg ttgccccggg gagcggggag
       61 aagcgcgcgg agtgcagccc cggcgcacca tcaagcgcat caacttccag cccggtatcg
      121 tcagccagta ctgccaaccc ggtggcagcc tgataggcaa aggctttcag gagcgcgttc
      181 tcaaggtatg tacggccggt ttcttccacc cgcgtgtcag gagaaacctc ctgcggggtc
      241 agcagttgca gcggcaggcc tgccaataga gcgctcaatt cacctatttt gcctttattg
      301 gtcgtcgcca gcagcagctg cataccttta ttattgttta tatttatcat aattataata
      361 ataaattgac ttcaactacc caaagtgcta taatcgtgat tgttaagatt atatcccgat
      421 taataaccgt gtgccgtggc caaccaacgt aacccattaa aaattaatgt cggcttcttt
      481 aataatctgc cgatcgggag ttttcgcgac attcattttg aaagtcccga tttgcacctg
      541 cctccagacc tggacgtaac caatttttca gggagtgttc atatttcgcg gaccccacag
      601 gggttgctgt ttgacggggc ttttcgcgcc ttcattcagg ctgaatgtgt ccgctgcctg
      661 gaaccttatg accaactgct cgagactgaa ttcagtgaag tgtacgctta taaatctcac
      721 tcctttactg aatccaacct tttcgttcct gatgatggga acatcgacct ctcgccggtt
      781 atcagggagt acctgatgct ggaaaacccg atcaaaccgc tatgtaaacc cgattgccag
      841 ggtttgtgcg tagtgtgcgg ggagaatcta aacatcgcaa cttgtgaaca ccaggccagg
      901 attaagatcg agtaaaccat ttctggatgc accaggggca agaatattaa aataaaggag
      961 actctcaggt ggcaaatccc ggaaggcgat ccaagagcgc ggaagtactg aatttattac
     1021 tggaaaaagc tgacgtgcag gggtacctca caacggaaga cttgatggag ttttacccag
     1081 acgtgaccag ggacgcagag cggattgaag cggtactcac tgccctgcgg cgccgaggtg
     1141 tggaaatatt ggatggtgag aacgactcag atatcactga caatgaaggt tacgcgcaag
     1201 taaccgacct ggactttacc tccacctttg agaacatctc ctctgacgac acgatcggcc
     1261 tttaccttaa ggaaatgtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1381 gcatccggca gatcgagagc aaagcgctca ggcgcctgcg ccacccgcgc cgctccaggc
     1441 aactgagaga atacctttaa agcgtcaacg gccatcctga tgcgggatgt ccgctttgcg
     1501 taagacctgg gattattaac ctatacgctg ttgagggttg gtttcctgct cccagcgcag
     1561 cactgcctcg tggatcaacg agcgcacctg gggatacgga acctcttcga tccccgaata
     1621 cttttctgcg ccaacaaata catccacact gtggttcggg ttttcttcca gcctgatgcc
     1681 cttatccgcc agttcggtgg tttgcagcat ctcctgcaga atggcgttta tttgctgggt
     1741 gattgtatac tcagcaacct ccgacgctgt gtacccggtc tcttcttcat cttcaggagg
     1801 cagagactgt tgcagctcgc tgatcacatc cgcttcttct tcttcggtgg gcaagaaagg
     1861 agaaaccgct acttcagcgg aggtctcctt cttctttatc atagaaatcg taattttcgc
     1921 ggctaacggg atcaggcagc ggctgataat gcgtattctc cacaacagtt gcctgcgggg
     1981 ctgtttcaac cggaggctcc gcccctaccc attcatttaa cagcgcattc aaccgtttca
     2041 acttagcttt ttgttccccg cttaatccat taacgctctt gtaaaagccc ccgtccattt
     2101 cagtgaggac ttttttggta ccaggagagt accacaagcg cgccacttca ccatatcctt
     2161 cttttagaat ttcaggggga ggttggcttt ttcgtttgcc ttctttgcct gaagacaaac
     2221 tggagagcaa caaaccaatg aacaatccaa taagggccgc cagcagtaga aggagcgtag
     2281 atccttgagc actcatttaa cacctcgaaa taagacctgt gaaaaataga aatacaataa
     2341 caccatcatt atccaatgaa aaagtaaata atgcaataac cctacaataa tgttttttta
     2401 gcctttttcc agcagctggc aagaaggaca aaaatgagtc ccccgctggc tgaccatgat
     2461 tcgttcgata ttgtcgccgc acaaggggca tttctcgcca ttgcgctgat acaccctgaa
     2521 gtaattctgg aatccccgcc gcggtaagcc cagtcgatgc tggcgccgtt ccgccgaata
     2581 ccttctgtta gcacctcgcg gatcgcccgc agcagggatt ctgcctggct gtagttgagg
     2641 gatgacgcct tctcaagcgg gtgtacgcgg gcgaggtgca gcgcctcgtc ggtgtatata
     2701 tttcccattc cagccaggaa cttttgatcc atgagaagcg gtttgatagc ccgcttcttt
     2761 ttatgtaaca tactatgaaa ggcgcctgcc gtcagctcag ggtcaaaggg ttctggccca
     2821 agagtaccca gaacttgctg agggtctttg agcagccagg ctcgaccgaa tttgcgtgtg
     2881 tcgttaaaaa ccatgcgcgc gtcatccgca aaattaaaaa tgatcctgtc gtgcagcccc
     2941 agggtggtct tatcacgggg ttccacccgg agatctccac tcatgcgcag gtggatgagc
     3001 atccaatctt tttcaagttg aaacactaaa aattt
//
LOCUS       DAOSPM010000092         2999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_32871_length_2999_cov_14.465014, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000092 DAOSPM010000000
VERSION     DAOSPM010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32871_length_2999_cov_14.465014"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLW25_07565"
     CDS             complement(<1..500)
                     /locus_tag="PLW25_07565"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOS54377.1"
                     /translation="MKLLQITGLFLLGYALGNFSPAYLIARKVGRFDIREKGSGNAGA
                     TNVIRTLGWRYGLLVFLLDALKGATAAGAGYWLGGETGLAAAAIGVVLGHDFPVVLNF
                     RGGKGIAATTGILLSLLPLPTLAGILVFVLVVLGTRMVSLGSLVFVFGMAVYVIVTQQ
                     PLMWVVV"
     gene            641..2164
                     /gene="zwf"
                     /locus_tag="PLW25_07570"
     CDS             641..2164
                     /gene="zwf"
                     /locus_tag="PLW25_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOS54378.1"
                     /translation="MTDSTSIVIFGASGDLSHRKLIPALYHLYLKGRLPKSFRVFGIA
                     GRPWTDEDLRRVSRDGLNQFSRLPVDENSWDTFSQTLSYLSGDISRAETYHQINERLS
                     LLEGGEANRMYYLATPPEYFLEIINNLAANGLLQEDDAWRRVVIEKPFGEDLESARSL
                     NQSIHAVMKEKQVYRIDHYLGKETVQNILVARFANAIFEPVWNRNFIDNVQITVAETV
                     GVGTRARYYDHAGVLRDMFQNHLIQLLTLVAMEPTSTYNADALRDEKLKVIRAIRPMN
                     RDEIMKNSVIGQYRGYQQEPNVAPGSRTPTYAAVRFYIDNWRWQGVPFYLRSGKKMTE
                     KVSEILIQFKKPPHMLFPMSGSDLEGRSNLLSMCLQPDEGIHLRFETKVPDTVAETRS
                     VNMNYHYSDVYDQSAIPEAYERLLLDVITGDASLFTRADQSELSWALFDPIIAGWEDP
                     AAPQPEEYEPGSWGPDGSERMLRAEGRQWLQTCGVHCEENHKTCDEKRVDPSRQQPW"
     gene            2239..>2999
                     /locus_tag="PLW25_07575"
     CDS             2239..>2999
                     /locus_tag="PLW25_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54379.1"
                     /translation="MKPALPCNNPPRCWSIPLLIFTAALLLFLSACQPAGPAASEPIN
                     ALSQETPPAQTPLLATATQHDTLSLSPTETSPPVPTQTTIVRTPGFWKELPILPEELS
                     VRAREIYQQGLAMGNDPRVYTKIGDCNSMSPDFLAGFGGQYDLGEDFAYLQPAIDYFN
                     KSYRLPNQATNPGTTTSRLLVSLWNNDECLPNEPMLECQYRVDNPSIAIIMLGTIDAK
                     THQRQPEVYEQNLRVIIEKTISLGILPVLATKADN"
BASE COUNT          752 a          881 c          717 g          649 t
ORIGIN      
        1 accaccaccc acatgagcgg ttgctgcgta acaatcacat acacagccat gccgaaaaca
       61 aacaccaatg accccaatga gaccatgcgc gtgcccagaa ccaccagcac aaaaacgagt
      121 atgcccgcca gggttggcag gggcagcaaa ctgagcagga taccagtggt ggcggcgatg
      181 cccttcccgc cgcgaaagtt taaaaccacc ggaaagtcat gtccaagaac caccccaatg
      241 gctgcggctg ccaatccggt ttctcccccc agccagtagc cagcgcccgc cgcagtggct
      301 cctttaagcg cgtctaacaa aaacaccagc aacccatagc gccagcccaa cgtgcggatt
      361 acattggtgg ccccagcgtt accgctgcct ttttcacgta tatcaaatct gccaaccttg
      421 cgggcaatca gataagcggg tgaaaaatta cccagcgcat agcctaacaa gaacaatcct
      481 gtgatttgga gcagtttcat aatacaaagt aacactgcaa cccgcggatt ggatacgccg
      541 ttctacccat cacttttctc tctcagggta cccgttttaa tggttgcctg ctcatgctga
      601 atctggatat aatgttctga aattagggaa aaggactgac atgacagact ccacatcaat
      661 cgtaatcttc ggcgcctctg gcgacctctc tcaccgcaag ttaattcccg ccttatatca
      721 cctgtatctc aaaggccgcc tgccgaaatc cttcagggtg ttcggtatcg cagggagacc
      781 ctggacggac gaagacctgc gccgggtttc acgcgatgga ctgaaccaat tctctcgtct
      841 gccggtggat gagaattcct gggatacttt ttcccaaacc ctgagctacc tttccggcga
      901 catctccaga gcagagacct atcaccaaat taatgagcgg ctgagccttc ttgaaggcgg
      961 ggaagccaat cgcatgtact acctggcaac acctccagaa tattttctag aaataatcaa
     1021 caacctggcc gcgaacggtt tgctgcaaga agacgacgcc tggcgccgcg ttgtgattga
     1081 aaagcctttc ggcgaagacc tcgaaagcgc ccgttcgctc aaccagagca ttcacgcagt
     1141 gatgaaagaa aaacaggttt accgtatcga tcactatctc ggtaaagaaa ccgtgcagaa
     1201 tattttagtg gcgcgtttcg ccaacgcgat cttcgaaccg gtgtggaatc gcaactttat
     1261 cgataacgtg caaatcaccg ttgccgaaac tgtgggggtg ggcacgcgtg cgcgttatta
     1321 tgatcacgct ggtgtgctgc gcgacatgtt tcaaaaccac ctcatccagt tgctgacgct
     1381 ggtagccatg gaaccgacca gcacctacaa tgctgacgcg ctgcgcgacg agaagctgaa
     1441 ggtcatccgc gccatacgcc ccatgaatcg tgatgaaatt atgaaaaaca gcgtcatcgg
     1501 tcaatatcgc ggttaccagc aagagcctaa cgtagccccg ggttcacgaa cccccactta
     1561 cgccgcggtg cgtttttata tcgataactg gcgctggcag ggcgtgccct tttacctgcg
     1621 ttcaggcaag aaaatgacag aaaaagtgag cgaaattctc attcagttta aaaaaccacc
     1681 gcacatgctt ttcccgatgt cggggagcga tttggaagga cgttccaacc tgctttcgat
     1741 gtgcctgcag cccgatgaag ggattcacct gcgttttgaa accaaggtac ccgataccgt
     1801 ggcggagaca cgttcggtta acatgaatta tcactacagt gatgtgtatg accagagcgc
     1861 cattccagag gcgtacgaac gtttactgct ggatgtcatc accggtgatg cttctctttt
     1921 cacccgcgct gaccaatctg aactttcctg ggcgctgttc gaccctatca tcgctgggtg
     1981 ggaagaccct gcagcgcccc agcccgaaga atacgagcct ggcagttggg gcccagacgg
     2041 cagcgaacgt atgttacgtg cagaaggccg gcagtggctg cagacctgcg gcgtgcattg
     2101 tgaggagaac cacaaaactt gcgatgaaaa gagagttgac ccttctcgtc agcaaccctg
     2161 gtagaaacag gtataattgc tgcgtttcat cttcaatcac tggatcattc tcattatcaa
     2221 aagggacttg ccattgttat gaagcctgcc ctgccctgta acaacccgcc gcgctgctgg
     2281 tccatcccac ttctcatttt taccgctgcc ctcctccttt ttttaagcgc atgccagcct
     2341 gccggccctg ccgcgtccga gccgattaac gcgttgtcgc aagagacccc gcctgcacaa
     2401 acaccactgc ttgctacagc tacacagcac gataccctct ctctctctcc cactgaaacc
     2461 agtcctcccg tccccaccca gaccaccatt gtgcgaaccc cgggattctg gaaagaactg
     2521 ccaatcctgc cggaagagct ttcagtaaga gcgcgcgaaa tttatcagca ggggttggca
     2581 atggggaatg acccgcgggt gtacaccaag attggcgact gcaatagcat gtctcctgat
     2641 ttcctcgccg gctttggcgg tcaatacgac ctgggcgaag atttcgccta cctccagccg
     2701 gcaattgact attttaataa atcgtaccgc ctgcccaacc aggctaccaa ccctggcacc
     2761 accacctcca gactgctggt ttcactgtgg aacaatgatg aatgcctgcc caacgaaccg
     2821 atgcttgagt gccagtaccg ggtggataat cccagtatcg ccatcatcat gctgggcacc
     2881 atcgacgcca aaacgcatca gcgccagccc gaggtttacg agcagaacct gcgggtcatt
     2941 attgaaaaaa ccatttctct tggcatctta ccggtattgg ccaccaaggc ggataacat
//
LOCUS       DAOSPM010000093         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_34589_length_2883_cov_12.702263, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000093 DAOSPM010000000
VERSION     DAOSPM010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34589_length_2883_cov_12.702263"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PLW25_07580"
     CDS             <1..979
                     /locus_tag="PLW25_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOS54380.1"
                     /translation="FTTDLKGNTPIPISDLLTGEGGRFTATPTPFQPVFQGINPPSVF
                     TSTPSVTPPPGTGGMIALGLARPASQVNILLLGSDWRPGQGYRTDVIMILSLIPDSNR
                     VTLLSFPRDLYVNIPGIGYERLNSAQAYGGFPLTAETLKTSFDASVDYYMMTDFSGFK
                     NIIDTLGGIQVYATSDLYDRCDLPQAYAKMCYIPAGWNTMNGETALWYARSRYSTSDF
                     DRTRRAQEVAVAITQKMISLNALNRAGELYQLFLSSVETDIPLDLIARLLPMAATAAD
                     NPSSIHRFTITEDQTTNYIVPATGAMVLLPDYDSIARTIQDALYPSLEH"
     gene            1064..1954
                     /locus_tag="PLW25_07585"
     CDS             1064..1954
                     /locus_tag="PLW25_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOS54381.1"
                     /translation="MDIQPELHITSETTFHPAIRSWEIFLLDQGRSLHTVKAFLGDMR
                     LFANYFPSGTTIGSITTDDINRFLGWLQHERHVPCSAKSLSRRITSTKSFFRWLSRSG
                     RIPSDPAEKVLQKTVISPLPDVLTPEEEVLALEAARALRVASKPDPRPYVLLSLLLST
                     GIKKGECLALTLNHIDLDSPKSPRLFVRYSASGSRYKERNIQLNEDWVEAYQEYLDVY
                     HPVEQVFPWSPRRLEYLLEDITIDAGLSKHISFAMCRWTCALNDWKSGMDKEALRQKL
                     GISKIQWRETSNKLRLLAGE"
     gene            complement(1991..>2883)
                     /locus_tag="PLW25_07590"
     CDS             complement(1991..>2883)
                     /locus_tag="PLW25_07590"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TilS substrate C-terminal domain-containing
                     protein"
                     /protein_id="HOS54382.1"
                     /translation="VEAYLSEIPIRPIFDQSNQDSSFFRNRIRNDLLPQLLTYNPQFK
                     KRILRMADAIALEDDLVMAQTRQAWAETVIETGEDFVVFDRAKTASLHPALRRRLMRE
                     GLTRLRSELRDMDYQVIEKGSHFFTDPCQSNTVHLIAGLEMFAHLKRRVVLAKKESPL
                     HGLWPQLTQKKALPLKIPGMVSLNEHWTLRSSRETIYINTGDPFVCQLNAELLADKLT
                     IDRFKAGDRFSPYGAAAQEMKLGDFWTNQGLAARARSNWPLIRSGEEIVWVPGFRIAD
                     RWRVKKETTSILRLELIKTE"
BASE COUNT          695 a          796 c          673 g          719 t
ORIGIN      
        1 tttcaccacg gacctcaagg gaaacacccc tattccgatc tctgacttgt tgacaggaga
       61 aggcggccga ttcaccgcta cccccacccc cttccaaccc gttttccagg gaataaatcc
      121 cccatctgtt ttcaccagca ctccctcagt gactcctcca ccaggtacag gtggaatgat
      181 tgcattgggg ttggcacgac ccgcgagcca ggtgaacatc ctcctgctgg gttctgactg
      241 gcgacccggg cagggttacc gcacagacgt aatcatgatc ctttcgctga tacccgatag
      301 taaccgagtg acattattat cttttccgcg cgacctgtac gtcaatattc cggggattgg
      361 ttacgagcgg ctcaactcag cccaggctta tggggggttc ccgctcaccg cggaaacgct
      421 caagaccagt tttgatgcca gtgtagatta ttatatgatg accgactttt caggttttaa
      481 aaacatcatc gataccctgg gcggcattca ggtatacgca acttccgacc tgtatgaccg
      541 ttgcgacctg ccacaggctt acgcaaaaat gtgttatatc ccggcaggtt ggaacacgat
      601 gaatggagag accgccttat ggtacgcgcg ctcacgttat tccacaagcg attttgatag
      661 aacccggcgc gcacaggaag ttgccgtagc catcacccaa aaaatgatca gcctgaacgc
      721 cctgaatcgt gctggtgagc tctaccaact ttttctttcc agtgtggaga cggatatccc
      781 gcttgattta attgcgcggc ttttacctat ggcagccacc gctgctgata acccctcctc
      841 catccatcgc ttcacaatta cagaagatca aacgacaaac tatattgtgc ctgcaacagg
      901 tgccatggta ctgttgcctg actatgattc gattgcccga acgatccaag acgccttata
      961 cccctctttg gaacattaat tgcatattga cattcgccaa taatccttgt acactgatat
     1021 taataggact atgcgaaatt aataattgaa agaaggctcc tttatggaca ttcaacctga
     1081 actccacatc acttcagaaa caacttttca ccctgccatc cgctcatggg agatattctt
     1141 gctcgaccag gggcgttctc ttcatacggt aaaagccttc ctgggagaca tgcgtttatt
     1201 tgccaactat ttcccttctg gcaccaccat tgggagtatt accacagatg acatcaaccg
     1261 tttccttggc tggcttcagc atgaacgcca cgtgccatgc agcgcaaaaa gcctgtcgcg
     1321 aaggatcacc tctactaaat ccttctttcg ttggctatca cgttccggac gtatcccgtc
     1381 tgacccggct gaaaaagtgc tgcagaagac ggtaatcagc cccttgccag acgtgcttac
     1441 acctgaagaa gaagtgttgg ccctcgaagc cgccagagcg ctgcgtgttg ccagtaaacc
     1501 cgatccccgc ccatacgttt tgctcagcct gttgttgtcc accggaatta aaaagggcga
     1561 gtgcctcgca ctcaccctca accatatcga ccttgacagc ccgaagagtc cgcgtctgtt
     1621 tgtgcgttat tcagccagcg gcagccgtta caaggaacgc aatatccaac tgaatgagga
     1681 ttgggtcgag gcttaccagg aatatctgga tgtataccat cccgtcgaac aggttttccc
     1741 ctggtctccg cgccgccttg agtacttgct tgaagatatc accatcgacg ctggcctatc
     1801 taaacacatt tcattcgcca tgtgccgctg gacctgcgcc ttgaatgatt ggaaatcagg
     1861 gatggataaa gaagccttgc gccagaaact gggcatttcg aagattcaat ggcgtgagac
     1921 cagcaataaa ttgcgcctgc tggcaggaga atgaccgggt tgttgcgcgc agagtggcag
     1981 caacaggctg tcactctgtt tttattaact cgagccttag aatgctcgtg gtctcttttt
     2041 ttacacgcca tcggtcagcg atacgaaatc caggtaccca cacaatctcc tcaccgctgc
     2101 gaatgagtgg ccagttactt cgtgcgcgcg ccgccaaacc ctggttggtc caaaagtcgc
     2161 ccagtttcat ctcctgggca gcggcgccat aaggcgagaa cctgtcaccc gctttaaaac
     2221 gatcgatggt cagtttatca gccaataatt cagcgttcaa ctggcaaaca aaagggtcac
     2281 cagtatttat gtaaattgtt tcacgagagc tgcgcagggt ccaatgctca ttgagcgaga
     2341 ccatgcccgg tatcttcagg ggtaacgctt ttttctgagt caattgcggc cataacccgt
     2401 gtaaaggaga ttcttttttt gccagcacca cacgcctttt aagatgcgca aacatctcca
     2461 aacccgcgat caggtgtaca gtgttgctct ggcatggatc tgtaaagaaa tggctgcctt
     2521 tttcaatcac ctggtaatcc atgtcccgca gttcactgcg caacctggta agcccttccc
     2581 tcatcaacct gcggcgtagc gcggggtgca gagacgccgt tttcgctcgg tcaaaaacca
     2641 cgaaatcctc gcccgtttcg ataacggttt ccgcccaggc ttgccgggtt tgagccatga
     2701 ccaggtcgtc ttccaaggca atcgcatcgg ccatcctgag gatgcgtttc ttaaactggg
     2761 gattataggt gagtagctga gggagcaaat cattacggat gcggttgcgg aaaaagctcg
     2821 aatcctggtt cgattgatca aatatgggtc tgatcggtat ttctgaaaga taggcttcaa
     2881 ctt
//
LOCUS       DAOSPM010000094         2864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_34901_length_2864_cov_14.795301, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000094 DAOSPM010000000
VERSION     DAOSPM010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34901_length_2864_cov_14.795301"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="PLW25_07595"
     CDS             <1..1470
                     /locus_tag="PLW25_07595"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOS54383.1"
                     /translation="TGEKTMLDQIKQQISRVKALGASYVDTRWYPVEETNSLLMWNGN
                     LKESSATFESGLGVRVLYHGAWGFSASSDTSDLAALYDRALDNARIASERLSLPVRLA
                     EKDAIQAKFVSPNKINPFEVPLSEKVAFLSEMDKKLDQPGVTQRASSLTFIRKQIIFV
                     DSEGSEIEKVITEVFAGLEVDGVDSEGGMYERKFSPFRRGDSRGWETIDLQHFSENAE
                     RIVRELNDTLNADPCPVDDRSVILLPGIMYLQTHETIGHALELDRILGYELAFAGGSF
                     VTLDDFGKLRYGSEKLTARADATLPNTPGSFGFDDDGIPAQDNLLIDRGLLVGAISGR
                     QMVEEANARARRQIFHGSSGANRATSFYRVPIERMTNINIDPGNDGTLEEIIRNTEKG
                     IVLDGDRSWSIGSNREQFHFGTEAGWLVEDGRVTRAVKNATYKGATLPFWNSLTAVGD
                     ASTWEVYYVPNCGKGQPNQIMQLGHGVPVCRFDNVRIGE"
     gene            1473..2858
                     /locus_tag="PLW25_07600"
     CDS             1473..2858
                     /locus_tag="PLW25_07600"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOS54384.1"
                     /translation="MKDRILNTLDQLRRYALSKGYSVSIYFQEEDSSLMRFANSAISL
                     NTNEHLIRLNITACDGRKRASYGMITDLSRVEDMQRGVETAAEMVKHSMPLTYESTIP
                     VFREAFIDESGYDPSLAEMSNAEKLAFFNRVVADMESDEIKLSGVFSNGANTTAMVST
                     ATPYPLYIKTSDAQVSIVLAHQTLKWEIQSEQSAQKKDELSADAMRQELSCLMSHYMH
                     DTPQQLPLGVYDVVFGRAAIAEMLQFMSYIGIHGGLMKRGYSCLSKEDIGKKIFSEQF
                     TLIDDPTRLETFPFRRDLSGMTRQPFTLVENGIFKGFIWMQDDADEFGEQPTGHTIPH
                     LSLSMAGGSQAVGSLEDLFNQPRDHDLLYIPFLHYMNIVNPSKGILTSSSRFGAVLLK
                     KDGQTIIPYNVRLTQSLLDIFGDKVDWLSAETSAYNLSTSYDARNPTAFIVPRFARVN
                     GLEISHSNSSY"
BASE COUNT          715 a          789 c          761 g          599 t
ORIGIN      
        1 acaggagaaa agacgatgct tgatcaaata aaacagcaga tcagccgggt caaggcgttg
       61 ggagcgagtt acgtggacac acgctggtac ccggtggaag aaaccaattc attgttaatg
      121 tggaacggca atttgaaaga atcgagcgct acatttgaaa gcgggctggg cgtgcgcgtg
      181 ctgtatcacg gcgcctgggg tttttcagcc tcctcagata ccagtgacct cgcagccttg
      241 tatgacaggg ctcttgataa cgcgcggatt gcctccgaga gactttctct gccagtgaga
      301 ttggcggaga aagacgccat ccaggcgaaa tttgtgagcc ccaacaagat aaacccgttt
      361 gaagtcccat tatctgaaaa ggtcgccttc ttgagcgaaa tggacaaaaa actggatcaa
      421 cccggtgtaa cccaacgggc gagcagcctg acctttatac gcaaacaaat catcttcgtc
      481 gactctgaag gcagcgaaat cgagaaggtc attaccgaag tcttcgccgg gctcgaggtg
      541 gatggtgttg atagcgaggg cgggatgtat gaacgcaagt tcagcccctt ccggcgcgga
      601 gacagccggg gctgggagac cattgacctg cagcacttca gcgaaaacgc ggagcgcatt
      661 gtacgcgaac tcaacgacac gctcaacgcc gatccgtgcc cggttgacga ccgctcggtc
      721 attctcctgc ccggtatcat gtacctgcaa acccacgaaa ccatcggtca cgcgctggaa
      781 ctggatcgta ttttgggata tgagctggct ttcgccggcg ggtcgttcgt caccctggat
      841 gattttggta aattgcggta cggctctgaa aaattgaccg ccagggcgga cgccacgctc
      901 cccaacacgc caggaagttt tggttttgac gacgacggca ttcccgcgca agacaatctg
      961 ctcattgacc ggggtttgct ggttggcgcg atcagcgggc gccagatggt agaagaagcc
     1021 aacgcccggg cacgccggca aatttttcat ggcagcagcg gggcaaaccg cgccacctcc
     1081 ttttaccggg tgccgatcga acgcatgacc aatatcaata tcgaccctgg caacgacggc
     1141 acgctggaag aaatcatccg caatacagaa aagggaatcg tgctggatgg agaccgcagc
     1201 tggtccattg gttccaaccg agagcaattc cactttggaa cggaagcagg atggctggta
     1261 gaggatggtc gggttacccg ggcggtcaag aacgcgacct acaaaggcgc cacgctccca
     1321 ttctggaatt cgctcaccgc agtgggtgac gcctccacct gggaagttta ttacgtgcct
     1381 aactgcggta aaggacaacc caaccagatc atgcagttgg gtcatggcgt gccagtgtgc
     1441 cgttttgaca acgtcaggat tggagaataa caatgaagga taggattctc aatacattag
     1501 accagttgcg ccggtacgcg ctttcaaagg gctacagcgt ctccatctat tttcaagaag
     1561 aagacagctc gctgatgcgc ttcgccaact cggccatttc gctgaatacc aatgaacacc
     1621 tgatccgcct gaatatcacc gcttgtgatg gtcgaaaacg cgccagttac gggatgatca
     1681 ccgacctttc cagggtcgaa gatatgcaaa gaggagtaga gaccgcggca gaaatggtga
     1741 aacacagcat gcctctcacc tatgaatcaa cgatccccgt cttccgagaa gcgtttatcg
     1801 acgagagcgg ttacgacccc tccttggctg agatgagcaa cgctgagaaa ctggctttct
     1861 tcaaccgcgt cgtcgcggac atggaaagtg acgaaatcaa actttccgga gtcttttcta
     1921 acggcgcgaa cactaccgcc atggtcagca ccgccactcc gtaccccctg tatatcaaaa
     1981 cctccgacgc gcaggtgagc attgtattgg ctcaccagac cctcaaatgg gaaattcaat
     2041 cggaacaatc cgcgcagaag aaggatgagc tctctgcgga tgccatgcgc caggagctgt
     2101 cgtgcctgat gtcacactac atgcacgaca ctccccagca gctcccattg ggggtttacg
     2161 atgttgtttt cgggcgcgcc gccatcgcgg agatgctgca attcatgagc tacatcggca
     2221 tccacggcgg gttaatgaag cgcggctatt cctgcctgag caaagaagat atcgggaaaa
     2281 agattttctc tgaacaattc acccttatcg atgaccccac ccgcctggaa accttcccgt
     2341 ttcgccgcga cctgagtgga atgacacgcc agcccttcac cctggtggaa aacggtatat
     2401 tcaagggctt catctggatg caggatgacg cggatgaatt tggcgagcaa cccaccgggc
     2461 acacgatccc ccatttgagc ctgagcatgg caggcggctc tcaagcggtg ggctctttgg
     2521 aagacctttt taaccagccg cgcgatcacg acctgcttta catccccttc ctgcattaca
     2581 tgaatatcgt caacccgtcc aaaggcatcc tcaccagcag ttcacgcttt ggcgcggtct
     2641 tgttgaaaaa agatgggcag accattattc catacaatgt ccgtctcact cagagcctgt
     2701 tggacatttt tggagacaaa gtggattggc tatcagccga aacgagcgcg tacaacctct
     2761 caacgagcta tgatgcgcgc aaccccaccg ctttcatcgt tccgcggttc gccagggtca
     2821 acgggctgga aatttcacac tccaactcat catactaaat ttac
//
LOCUS       DAOSPM010000095         2859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_34988_length_2859_cov_11.504280, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000095 DAOSPM010000000
VERSION     DAOSPM010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34988_length_2859_cov_11.504280"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1602)
                     /gene="murJ"
                     /locus_tag="PLW25_07605"
     CDS             complement(64..1602)
                     /gene="murJ"
                     /locus_tag="PLW25_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOS54385.1"
                     /translation="MKFSRLTKISLLLAAFFFLDKVLAFARQVLIARQFGLSAELDAF
                     NVANNIPDLLFALISGGALAIAFIPVLSEVLTRSGQKPAWLLFSRIANLAFLVTVALA
                     TLVAVFAAPMVQAELGIAPGFTVAQQHLVIHLMRLNLIATIIFSISGLVMAGLQANQH
                     FLLPAMAPLFYNIGQILGVGILSPEQGYRLGPLTLPAYGLGVQGLVYGVIIGAVLHLA
                     IQVPGLAKYHFKWSISLNLRDEAVRKVLRLMGPRLLTMFFIQLIFLIRDNLASRLAEG
                     AVTSLTYGWMIQQVPETLIGTAIGTALLPTLSEHFSRDEKQAFSERIDKAFKALLALT
                     LPVTAVLAAGLRPLLGLAFDFGAAGTRMLAWVTAAFMLGLFGHSLKEVSARAFYASQN
                     AWVPLATAGGNVLFYTLCGALFYRPLGAPGIALTDSLIFSLEALVLLALLIRSGRVSI
                     VPSSTAWRALLGCVSAGAVTWLGVTLLEAHAHPLISGSVPMFAGILVSLPWIWREIKL
                     LTQL"
     gene            1752..2429
                     /locus_tag="PLW25_07610"
     CDS             1752..2429
                     /locus_tag="PLW25_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOS54386.1"
                     /translation="MTKKKGTLYIVATPIGNLGDITLRAIETLKQVNTVICEEHKPGS
                     TLLKKLQLTGKELVLLNEHNEAEVAAQLLTRLLNGESLALISDCGTPVFSDPGAYLIQ
                     LASSARVVVSPLPGASSLMAALSLLDTRIERFVFAGFLSRDPHKRKQELTRYRGFGMP
                     VVIMDTPYRLTALLEDVIKVFGKGQWVMVAFDLTLPGEMVFRGEAESVAKQVGGKKGE
                     FVLVVGR"
BASE COUNT          777 a          696 c          811 g          575 t
ORIGIN      
        1 gtttgttttc gacatgagcg taattatacc gtaagccagt ggagggtcaa attgctgcgt
       61 ttttcataat tgggtgagca gttttatttc gcgccagatc caggggaggc tgaccaggat
      121 gccggcaaac atgggaactg aaccagatat caacgggtgc gcatgcgctt cgagcagggt
      181 cacccccaac cacgttacag cgccggcgct tacgcatccc agcagcgcgc gccaggcagt
      241 ggagctggga acaatcgaca ccctgccact gcgtatgagc agcgccaata acaccagcgc
      301 ttccagtgaa aaaatcaacg aatcggtgag ggcgatgcct ggcgcgccca gaggacggta
      361 aaacagcgct ccgcaaagcg tgtaaaagag cacattaccc cctgcggttg ccagcggcac
      421 ccaggcgttt tgactggcat agaaggcgcg cgcgctgacc tccttgagag aatgaccgaa
      481 caagcccagc ataaacgcgg cggtgaccca ggcgagcata cgggtacccg cagcgccaaa
      541 atcgaaagcc aagccgagca aaggcctgag ccctgctgcc agaacagctg tgacgggcag
      601 cgtgagcgcc agcagcgcct taaaggcttt gtctattctt tcgctgaacg cctgcttttc
      661 atcgcggctg aagtgttctg aaagcgtggg taaaagagcc gtgccgatgg cggtgccaat
      721 gagcgtttca ggcacctgct ggatcatcca gccataagtc aacgaggtaa ccgccccctc
      781 tgccagtcgt gatgccaggt tatcgcggat gaggaagatg agctggataa agaacatcgt
      841 tagcaggcga gggcccatca gacgcagcac cttgcgaacc gcttcgtcac gcaggttcag
      901 gctgatgctc cacttgaaat ggtattttgc cagcccggga acctggatgg ccaggtgcaa
      961 taccgcgccg atgatcaccc cataaaccag cccctgtacg cccaacccgt aggcaggcag
     1021 ggtgagcggg ccaagcctgt aaccctgctc aggcgaaagg atgccgaccc ccaagatttg
     1081 accgatgttg taaaaaagcg gcgccattgc tggcagcaga aaatgctggt tcgcctgcag
     1141 accggccatc accaagccgc tgatggaaaa gatgatggtc gcgatgagat tcaggcgcat
     1201 caggtggatc accaggtgct gttgcgcaac ggtgaaaccg ggagcgatac ccagttcagc
     1261 ctggaccatt ggcgcggcga agaccgccac cagggtggcc aatgcgacgg tgacaaggaa
     1321 agccaggttg gcgatgcggc taaagagcag ccaggcgggt ttttgccctg accgggtcag
     1381 cacttcagaa agcacaggga taaaggcaat ggccagcgca ccgcctgaga tgagcgcgaa
     1441 aagcaggtca gggatgttat tagccacatt gaaggcatcc agctcggcag aaagaccgaa
     1501 ctgtcgagcg ataaggacct gacgcgcaaa cgctagcacc ttgtcaagga agaaaaaagc
     1561 agccagaagc aacgaaattt tggtcaggcg ggagaatttc atatctgcac cagagatgaa
     1621 atggtaacat aaaaaatgaa atcagagacg attttgccag gagagagtaa gcggcatggg
     1681 aggaaaaacc ggcggtgaag aatcggttac aatggttttg gctgccataa atccgaaagg
     1741 gagaggatga tttgaccaaa aagaagggca ccttgtacat tgtcgccaca cctattggga
     1801 acctgggcga catcactcta cgcgcgatcg aaaccttgaa acaggtgaac acggtgatat
     1861 gcgaagagca taaaccgggc agcacgctgt taaaaaaact gcaactgacc ggcaaagaac
     1921 tggttctctt gaatgagcac aatgaagccg aggtcgccgc gcaactgctg acgcggctgt
     1981 tgaacggtga atcgctggct ttgatttctg attgcggcac gccggtattt tctgacccgg
     2041 gggcatacct tatccagctg gcttcaagcg cgcgagtggt tgtttcgccg ctgccggggg
     2101 cgtcatcctt gatggcagcg ctttcgttgc tggataccag gattgagcgc ttcgttttcg
     2161 ctgggttcct ctcacgcgac ccgcataaaa ggaaacagga actgacacga taccgtgggt
     2221 ttggtatgcc tgtggtcatt atggatactc cttaccggct gaccgcattg ctggaagatg
     2281 tgataaaggt gtttggcaaa ggtcaatggg tgatggtggc attcgacctc accctgcctg
     2341 gggagatggt cttccgcggc gaagcagaaa gcgtcgccaa acaggtgggc ggaaagaagg
     2401 gcgaatttgt actggtggtg ggaaggtaaa agttccgggc agatcgagcc taaaaacacc
     2461 cgataaacgg ttatgcatgg aagtacgctg cacgaaaaaa gggctgaagg taactgctgg
     2521 cagtaaagcc tgtgtgtaga aaaagaaggc ttaccgggat tccacgtagt caaatcagtc
     2581 aaattacaga atttggatag aatcccaact attggtaaaa agacaagctt tttgaatcaa
     2641 aattacttga atttatagcc aaaataacca cttattacct actttcgatg cttatttggt
     2701 ttttcggcat tcgccggatt tcactaaata gtgaatcgga tatttttggt agagatacaa
     2761 tgtcaaaatt tttcgtgtaa gaaaagatgt gtcttgttgt aactctcaac aaaatatcct
     2821 taactagaaa tccatgcaaa aacttttcct gaaaaaaaa
//
LOCUS       DAOSPM010000096         2719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_37384_length_2719_cov_12.125000, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000096 DAOSPM010000000
VERSION     DAOSPM010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37384_length_2719_cov_12.125000"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLW25_07615"
     CDS             <1..463
                     /locus_tag="PLW25_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOS54387.1"
                     /translation="RPLQSGDLLVIDWGARSNGYCADLTRTFAIGNIDPELKAIYEAV
                     KQANSTGRAASKPGATAGEVDLAARAVIENAGYGAFFTHRTGHGLGLEGHEPPYIFNG
                     NSLALRPGMTYTVEPGIYLPGRGGVRIEDNLVITHTGCETFSDLSRELMIL"
     gene            484..1248
                     /locus_tag="PLW25_07620"
     CDS             484..1248
                     /locus_tag="PLW25_07620"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOS54388.1"
                     /translation="MAKTLTMATAEPFFIPAGKTGCLLIHGFTGTPKEMRMLADALAL
                     EGYTVLAPRLFGHATNPRDMSRARADDWIASVEDGLNLLKGCTDTQVVMGLSMGGVLA
                     LIAAARFDCRAAVTFSAPCSLPHDPRAWLLPLIGRLNLPIKKGKPDWRDLAAAQEHKD
                     YPYYPSRAIIELDRLIKLMQGELENIRVPVMLVQSRADRGIPPESLETIYNLLPTAEK
                     TSFWVEDSGHVIICEPERERIFSQVGAFLKQVLKDA"
     gene            1245..2393
                     /locus_tag="PLW25_07625"
     CDS             1245..2393
                     /locus_tag="PLW25_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS54389.1"
                     /translation="MNRNLLLVTISLFAWGMGEGLFIYFQPLYLQEFGADPVMIGSIF
                     GVMGVAMAVFQIPSGYLSDRFGSRSIMWASWFLGTSAAWTMALANTLPTFVVGLILYG
                     LTSFVVAPMNSYITKVRGDWSVGRALTFPSGFYHLGAVLGPISGGLIADRLGLKTVYM
                     VAAIIFIISTIIVLFTSQAPETHHADQVDATTKGTLKNPRFFAFLVITLLSIFAIYLP
                     QPFTPSFLQNQQAFSRSMIGILGAVGSLGNAVATLALGSLPSVIGFIIGQAWVLVFSL
                     TFLFARVPFWFGFGYFFIGGYRLCRSMVLAISRTLVNPRQTGLAYGMVETMNAIAVII
                     APVIAGVLYKNNPLSIFSVSLVIISITIILNLTVLVRQKRGRLHEPEN"
     gene            2377..>2719
                     /locus_tag="PLW25_07630"
     CDS             2377..>2719
                     /locus_tag="PLW25_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS54390.1"
                     /translation="MSLKIDTFTSSMMSNNVVILHDDETRRAIIMDPSFEPEQVLTFI
                     NNNSLSVEMILCTHGHFDHFAGLAYLLTKLHPKPKVGLHEGDLALWRAGGGAVHFRIP
                     IAVPEDPDIFLT"
BASE COUNT          585 a          731 c          710 g          693 t
ORIGIN      
        1 gcgccctctc caatcaggag atttgctggt catcgattgg ggcgcgcgct cgaacggtta
       61 ttgcgcagac ctgacgcgca ctttcgcaat cggtaatata gaccccgagt taaaggccat
      121 ctacgaagcg gtgaagcagg ctaactcaac cgggcgtgct gccagtaaac ctggagcgac
      181 tgctggagag gtggatttgg ccgcccgagc agtgattgaa aacgcgggat acggcgcctt
      241 tttcactcac cgtactggtc atgggctggg gttggaaggc catgaaccgc cttacatctt
      301 caacgggaat tcactagcgt tacgcccggg gatgacctac acggttgagc caggcatcta
      361 cttgcccggg cgagggggtg tgcgtattga agacaacctg gtaattacgc ataccggctg
      421 cgaaacattc agcgaccttt cacgcgaact gatgattctc taagagaaca actggagctc
      481 caaatggcta aaaccttaac catggcgact gccgaaccct ttttcatccc cgcaggaaaa
      541 acaggatgcc tgctcattca tggttttacc ggcacgccaa aagaaatgcg catgctggca
      601 gatgcgctgg ctcttgaagg ttatactgtt cttgctccgc gactgtttgg tcatgctaca
      661 aatccgcggg acatgagccg tgcgcgtgct gacgattgga tcgccagcgt tgaagatggt
      721 ttgaacctgt taaaagggtg tacagacacg caggtcgtaa tgggcttatc tatgggcggt
      781 gtgcttgccc tcatcgccgc ggctcgcttt gattgccgcg cagcggttac cttttccgct
      841 ccctgctcac tccctcatga tccgcgtgcc tggttgttac cgcttatcgg caggttgaat
      901 ctacccatca agaaagggaa acctgactgg cgcgacctcg cggcagcaca ggagcataag
      961 gattacccct attacccaag ccgggcgatc atcgagctgg acagattgat caagctcatg
     1021 cagggtgaac ttgagaacat ccgcgtacct gttatgctgg tgcaatcaag agcagaccgg
     1081 gggattccgc cagaaagcct tgaaacgatc tacaatcttc tgccaacagc cgaaaaaacc
     1141 agcttctggg ttgaagacag cggacatgtg atcatttgtg agccagaaag agaaaggatc
     1201 ttctctcaag ttggagcatt cttgaagcag gtcttaaaag acgcatgaat cgcaacctgc
     1261 tgctggtgac aatctccctg ttcgcctggg gaatgggtga agggctgttt atttactttc
     1321 aaccgttgta cctgcaggaa tttggcgcgg acccggtaat gattggcagt atttttggcg
     1381 tgatgggtgt cgcgatggcg gtcttccaaa taccctcagg gtatctttca gaccgtttcg
     1441 gttccaggtc gatcatgtgg gcttcatggt ttttaggaac ctctgctgcc tggacaatgg
     1501 cgctggccaa caccctgccc acgttcgtgg tcggcctgat tctttacggt ctgaccagtt
     1561 ttgtggtcgc ccctatgaac agctatatca ctaaagtgcg cggcgattgg agcgtgggac
     1621 gggcgcttac cttcccttca ggtttttacc accttggggc ggtgctgggt cccatcagcg
     1681 ggggattgat cgccgaccgc ctggggttga agaccgttta tatggtagcc gccattattt
     1741 ttataatctc caccattatc gtccttttca cctctcaagc gccagaaacc catcacgcgg
     1801 accaggttga tgccaccaca aaggggacat tgaaaaatcc gcgtttcttt gctttcctgg
     1861 tgatcaccct gctgagtatc ttcgccatct atttgccgca acccttcacc cccagtttcc
     1921 tgcaaaatca acaggcgttt tcgcgctcca tgatcgggat cctcggcgct gtcggcagcc
     1981 tggggaacgc cgttgccacc cttgccctgg gaagtttgcc ctcggtcatc ggctttatca
     2041 tcggtcaggc ctgggtgctg gtcttttcac ttacatttct tttcgcccgc gtgcctttct
     2101 ggttcggctt cgggtatttt ttcatcggcg gttacaggct gtgccggtcc atggtgctcg
     2161 ccatctcccg aaccctggtt aacccacgcc agaccggcct ggcttacggt atggtagaga
     2221 ccatgaacgc catcgcagtt ataattgcgc ctgtaatagc cggggtgctt tacaagaata
     2281 acccattgtc gattttctct gtttccctgg ttattatctc aatcacgatt atcttgaacc
     2341 tgaccgtcct tgtaagacaa aaaagaggtc gcttgcatga gcctgaaaat tgataccttt
     2401 acgtccagta tgatgagtaa caatgtggtc atcctgcatg atgatgaaac ccgccgtgct
     2461 atcatcatgg atccttcttt tgagccagag caagtgctca catttatcaa caataactcg
     2521 ttgtcagtgg aaatgatact ttgtacccac ggacattttg atcacttcgc tggtctcgct
     2581 tatctgctta ccaagctgca ccccaaacca aaggtcggtc tgcacgaggg cgatctggct
     2641 ttgtggcggg ccggaggagg ggcggttcat tttcgcatac caatagccgt ccccgaagac
     2701 cctgacatct tcctcacag
//
LOCUS       DAOSPM010000097         2523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_41202_length_2523_cov_14.468801, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000097 DAOSPM010000000
VERSION     DAOSPM010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41202_length_2523_cov_14.468801"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLW25_07635"
     CDS             <1..321
                     /locus_tag="PLW25_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54391.1"
                     /translation="EAQQITGWFTIPIVIPLWFTNVFMFNPNGVLSVAMSLFPVTAPI
                     ALPFRAAFTTVPQWQIILSITLLCLLAAFALWLSGRIFRLGMLRYGKRVRLREAFSKS
                     GSKP"
     gene            318..1550
                     /locus_tag="PLW25_07640"
     CDS             318..1550
                     /locus_tag="PLW25_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS54392.1"
                     /translation="MKKTLLILKNELITALTRKSFLITLVLLPLTSFIILLVISGLQK
                     SGQDTSAMLENLFVPASLSSAEGFVDNSGLMKEIPDEIANALIRYNTEEDARAALNAG
                     LISAYFIVPDDYLEKGDVFYIRPDFNPMGSTQQSSSIKSLIAFNLSGGNPALAYRLQD
                     PINAIEVSLSNQEQRDSDHWLTFFLPYIITFLFYIVILTSSSLLLNSISTEKQNRVME
                     ILLTSLTPSQVLTGKIIALGLTGLLQTVVWLGAGLLMLRYSGRAFALSAAFQLPLSIM
                     AWGVLFFIFGYAVFASLMAGIGALVPNLREGSQLTTVVILPMIIPLVFISALINTPNS
                     PLSVFLSMFPLTAPVSMMTRLSATQVPFWQASLALLLLALTAVFLVRASASLFKAQNL
                     LTGQTFTVAGFLKALAGK"
     gene            1601..>2523
                     /locus_tag="PLW25_07645"
     CDS             1601..>2523
                     /locus_tag="PLW25_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54393.1"
                     /translation="MQRKKLVIILVLGFLMAAVAETLLLVSMINTRNKTSADRSGQPQ
                     QLDATIPSSTEPALGVPDGIGPALVEVYTKQNALAAISTERMFARLTELTRIQPHSGW
                     RGAGTTGEKEAFDYLESRLTELEWLTSLGMSSERERFNIFLGTEVHQSSLFLTLGAHT
                     YEVPADATRGSRDNPAAALRMDSDGFFNPPYEDPVEVTGFPLLIPDAAALNSLEKRSY
                     QNEVFLVDYALVDTENPQALSNALSLLRLNPAAIVLITRFSNTGGQTHGTFAGDGGGV
                     LQRVETPGALPLLFIEFENLSPLGIHDWDEM"
BASE COUNT          544 a          735 c          613 g          631 t
ORIGIN      
        1 gaagcgcaac aaataaccgg ttggttcacc attcccatcg ttatcccgtt atggttcacg
       61 aacgttttta tgttcaaccc caacggcgtg ctctcggttg caatgagcct tttcccggta
      121 acagcgccaa tcgcgctgcc tttccgcgcg gcgttcacca ccgtacctca atggcaaata
      181 atcttatcta tcaccctgct gtgcctgctg gccgcctttg cgctctggct ttccgggaga
      241 atcttccgcc tgggaatgct gcgttatgga aaacgcgtac gcctgcgcga agccttctcc
      301 aagagcggaa gtaaaccatg aaaaagaccc ttctgatatt aaagaacgaa ctcatcactg
      361 ctcttacccg caaatccttc ctgatcactt tggttttatt gccccttacc agttttatca
      421 ttttgctggt gatatcaggt ctgcagaaat ctgggcagga caccagcgca atgctggaaa
      481 atttgtttgt tcccgcgtcg ctgtcttcag ctgaaggttt cgtggataat agcgggctga
      541 tgaaagagat accggatgag attgccaatg cgcttatccg ctacaacacg gaagaagacg
      601 cgcgggctgc gctcaacgcg ggactcatca gcgcttattt catcgttccc gatgattacc
      661 tggagaaagg tgatgttttc tatatccgtc cggattttaa cccgatggga agcacgcagc
      721 agtcttcctc gattaagtcc ctcatcgcct tcaacctcag cggcggtaat cctgccctgg
      781 cttacaggct gcaggacccc atcaacgcta tcgaagtgag cctttctaac caggaacaac
      841 gagatagtga ccactggctc acattctttt taccgtacat catcaccttt cttttttata
      901 tcgtcattct cacctcttcc tcgctgctgt taaacagtat cagcactgaa aaacaaaacc
      961 gggtaatgga aatcttgttg acctcactca cccccagcca ggtactcacc ggcaagatca
     1021 tcgccctggg gttgacaggg ttgctgcaaa cggtggtgtg gctgggcgcg gggctgctga
     1081 tgctgcgtta ctctggtcgc gcttttgcgc tcagcgcagc gtttcagctg ccattgtcga
     1141 taatggcctg gggcgtgctg tttttcattt tcgggtacgc ggtttttgcc agtctcatgg
     1201 cgggtatcgg cgcgcttgtt cccaacttgc gcgagggatc acaattaacc accgtggtca
     1261 tcctgccaat gatcatccca ctcgtgttca tcagcgcgct gattaacacc ccaaacagcc
     1321 cgctctccgt atttctcagc atgttcccgc tcactgctcc tgtaagcatg atgacccggc
     1381 tttccgccac gcaggtgccg ttctggcagg cgagcctggc cctgttatta ctagcgctta
     1441 ccgctgtgtt cctggtgcgt gccagtgctt cgctttttaa ggctcaaaac ctcttgaccg
     1501 gtcaaacctt tactgtagca gggtttctca aagccctggc agggaagtaa ttcacaccat
     1561 ccttcatcgt cttcatcacg cctttttgaa ggaggtttgc gtgcaacgca aaaaactggt
     1621 cattatcctg gtccttggct tcctgatggc agccgttgcc gaaaccttgc tcttggtttc
     1681 tatgatcaac acccgcaaca aaacaagcgc ggaccgcagt gggcaaccac aacagctcga
     1741 tgccacaatc ccttcctcga cagaacctgc gcttggcgtt ccagacggga ttggacctgc
     1801 cttagtggaa gtttatacaa aacaaaacgc cctggcagcc atttctactg aacgcatgtt
     1861 cgcgcgcctc acagaactta cccgcatcca gccgcattca ggctggcgcg gcgcgggtac
     1921 caccggggaa aaagaagcct tcgactacct ggaatccagg cttacggaat tggaatggct
     1981 cacgagcctg ggaatgtcat ccgaaagaga gcggttcaac atcttcctgg ggaccgaagt
     2041 tcatcaatcc tctcttttct tgactttggg agcgcatacc tatgaggttc ccgctgacgc
     2101 aacccgcggc agccgcgaca accccgctgc agccttgcgc atggactctg acggcttttt
     2161 taatccaccc tatgaagacc ctgttgaagt cacaggtttc ccgctgctta ttccagatgc
     2221 cgccgcgctg aacagcctgg aaaaaaggag ttatcaaaac gaggtcttcc tggttgatta
     2281 cgcgctggtc gatacagaga acccccaggc gctgagcaat gccctctctc tgctgagatt
     2341 gaaccccgct gccattgtgt tgatcaccag gttttccaac actggcgggc aaacgcacgg
     2401 aaccttcgcc ggggatggcg gcggcgtgct gcaacgggtg gagaccccgg gcgccctgcc
     2461 cctcctgttt attgagttcg aaaacctctc cccgttggga atccatgatt gggatgaaat
     2521 gaa
//
LOCUS       DAOSPM010000098         2478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_42213_length_2478_cov_14.323979, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000098 DAOSPM010000000
VERSION     DAOSPM010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42213_length_2478_cov_14.323979"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLW25_07650"
     CDS             complement(<1..356)
                     /locus_tag="PLW25_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOS54394.1"
                     /translation="MPNYDVKELNLAEGGRLKIDWAEREMPVLRQVKERFRREQPLKG
                     LRISACLHVTTETANLMKTLQAGGADVVLTASNPLSTQDEVAASLVSHDEIEVYAVKG
                     EDNVTYYKHINAALDH"
     gene            complement(378..1340)
                     /locus_tag="PLW25_07655"
     CDS             complement(378..1340)
                     /locus_tag="PLW25_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOS54395.1"
                     /translation="MAIVISGSVAFDYLMTFPGYFRENILPDKLDKISLSFLVDNMTR
                     QRGGCAPNIAYSMALLGEKPKVLATAGIDFAEYRSWMEQNGIDATYIREIPGKYTASF
                     FVNTDLENNQIASFYTGAMADAARFTLSEMPRDEVDYLVISPNDPAAMRKLTDECVSM
                     GIPYLFDPSQQVARNPHDDLRIGVENAHAVFCNEYEFDLLIKHTGLSAAQIEGLVQLL
                     VITRGEHGATVKAEGREYLIPVVPAARIADPTGVGDAFRGGFLRGYRLGLDLQTCGQM
                     GALSAAYCLEQNGPSNHFYTKQEYINRYRTFFDDGGTLDILNQP"
     gene            complement(1346..>2478)
                     /gene="mtnA"
                     /locus_tag="PLW25_07660"
     CDS             complement(1346..>2478)
                     /gene="mtnA"
                     /locus_tag="PLW25_07660"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOS54396.1"
                     /translation="ILHSPLFHVKLSGKGRSRPRAFFSELRTIEWLPESREVRLIDQR
                     RLPAALEYLTIQTPEQMVEAIRTMAIRGAPALGVAGAFGLALQAARYNRAGGKDLRSL
                     VHAAAVMLVNSRPTAVNLAWGVEQITSLLDEQTHSESELVERIISNAQALAEADVRTN
                     LRLAENGASLIDDGDTIIHHCNTGALAVVDWGTALGAIRFAHEQGKRVHVLVDETRPR
                     LQGSRLTAWECEQYGIPYEIITDNAAGFFLQSGRVHKVFFGADRVAANGDVVNKVGTY
                     MLSLAAHANQVPVVCVFPLSTLDLSIAEGRYIPIEERDAAEVLNLRVDGQAIAPATAR
                     ALNPAFDLTPHALISAWVTEAGVVRAPFAQNLAQSRYNQKMD"
BASE COUNT          528 a          715 c          694 g          541 t
ORIGIN      
        1 ttatggtcaa gcgcggcgtt gatgtgcttg taataggtaa cgttgtcttc gccctttacc
       61 gcgtacacct cgatctcatc atgagagacg agcgaagccg ccacctcgtc ctgcgtggaa
      121 agcgggttag acgcggtgag caccacgtca gccccaccgg cctgcagggt cttcatcagg
      181 ttggcggtct cagtagtcac gtgcaggcag gctgaaatac gcagcccttt aaggggctgt
      241 tcacggcgaa aacgctcttt cacctggcgc agcaccggca tttcgcgttc tgcccagtct
      301 atcttcaggc gtccgccttc ggccaggttc aattctttta catcatagtt cggcatgatt
      361 cctccaaatg gttgtcttta aggttgattt aaaatatcca aagtgccgcc gtcatcaaaa
      421 aaggtgcggt agcgattgat atattcctgc ttggtgtaaa aatggttgct gggtccattc
      481 tgttccaggc agtaggcggc ggaaagcgcg cccatttgac cacaggtctg caggtccagc
      541 cccagtcggt agccgcgcaa gaagcctccc ctgaaagcat cgcccacgcc agtggggtcc
      601 gcgatgcggg cggccggcac caccgggatg agatactcgc gtccctccgc tttcaccgta
      661 gcaccgtgct caccgcgggt gatcaccagc aactgcacca gcccctcgat ctgcgcagct
      721 gagaggccag tatgctttat cagcaggtca aactcatatt cattgcaaaa aaccgcgtgc
      781 gcgttttcaa caccgattct caagtcgtca tgtggattcc tggccacctg ctgcgatgga
      841 tcaaacaggt acgggatacc catggaaacg cattcgtcag tcagtttacg catggcggcg
      901 gggtcgttgg gagaaataac aaggtagtcc acctcgtcgc gcggcatttc tgagagggtg
      961 aatcgcgccg cgtcagccat ggcgccggtg taaaaactgg cgatctgatt attctcgaga
     1021 tccgtgttga caaagaaaga agcggtgtat ttgccgggta tctcgcggat ataagtggcg
     1081 tcgatgccgt tttgttccat ccagctgcgg tactcagcaa aatcgatgcc agcggtggcg
     1141 agcaccttgg gtttttctcc cagcagcgcc atagagtaag cgatgttcgg cgcgcaaccg
     1201 ccgcgctggc gggtcatgtt atccaccagg aatgaaaggc tgatcttatc cagtttatcc
     1261 ggcaggatgt tctcgcggaa ataacccggg aaggtcatca ggtaatcaaa agccacagaa
     1321 ccgctaatca caattgccat attgattaat ccattttctg attatatctg gattgcgcca
     1381 agttttgcgc aaaaggcgcg cggaccacac cggcctcggt aacccaggcg ctgatgagcg
     1441 cgtgcggggt gaggtcaaag gcaggattga gcgccctggc agtcgctggg gcgatggcct
     1501 gtccgtcaac ccgcaggttc agcacctctg cagcgtcgcg ctcctcgatc gggatgtacc
     1561 tgccttcagc aatggacaga tccagcgtac ttaaagggaa aacgcacacc accggcacct
     1621 ggttggcgtg cgccgccagg ctgagcatgt aggtgccaac cttgttgacc acatccccat
     1681 tggctgccac acggtctgcg ccgaagaaca ccttgtgaac tcgaccgctt tgcaaaaaga
     1741 accccgcggc attatcagtg atgatctcgt aggggatgcc atactgttcg cattcccagg
     1801 cggtgaggcg agagccctgc aggcgcgggc gggtctcgtc taccagcaca tgcacacgtt
     1861 tgccttgctc atgggcaaag cgaatggcgc caagcgcggt accccagtca accacagcca
     1921 gcgcccctgt attacagtga tgaatgatgg tgtcgccgtc atcgatcagg ctggcgccgt
     1981 tttcagccag gcggaggttg gtgcgcacgt cagcctcagc cagcgcctgg gcattgctga
     2041 tgatacgctc cacaagttcg ctttctgagt gggtttgctc atccagtaga gacgtgatct
     2101 gctccactcc ccaggccagg ttcaccgccg ttgggcggga gtttaccagc ataaccgcag
     2161 cggcgtgcac gagcgaccgg agatcctttc cacccgcgcg gttatacctg gctgcctgca
     2221 gcgccaatcc aaaagcgccg gccacgccca gggcgggagc cccgcggatg gccatggtac
     2281 gaattgcttc caccatttgc tctggcgtct ggatggtgag gtattctaaa gctgccggca
     2341 gccgacgttg gtcgatcagc cgaacttccc tgctctccgg taaccattcg atcgttctca
     2401 attcactaaa aaacgccctc ggtcgagagc gtcccttccc actaagttta acatggaata
     2461 aaggggaatg caatattt
//
LOCUS       DAOSPM010000099         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_43959_length_2405_cov_15.120000, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000099 DAOSPM010000000
VERSION     DAOSPM010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43959_length_2405_cov_15.120000"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLW25_07665"
     CDS             <1..207
                     /locus_tag="PLW25_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54397.1"
                     /translation="GISETITNVNGGAIALGHPLGCSGARILTTLLHEMKRRAPSAPR
                     PYYGMAMLCVGVGQGEATIVEWAA"
     gene            403..645
                     /locus_tag="PLW25_07670"
     CDS             403..645
                     /locus_tag="PLW25_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOS54398.1"
                     /translation="MPPKGRVVVSEIYCKGCELCVNACPQSVLGLDHEHLTAKGYHPA
                     VLIAEGCTGCGICAVVCPDAAITVYRIRTGATRELD"
     gene            656..1768
                     /gene="vorB"
                     /locus_tag="PLW25_07675"
     CDS             656..1768
                     /gene="vorB"
                     /locus_tag="PLW25_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOS54399.1"
                     /translation="MAKELMKGNEAVAEAAIRAGVTAYFGYPITPQTELLEYMSRRMP
                     ELGRVFVQAESELAAINMVYGAACTGRRVMSSSSSPGVSLMMEGVSYIAGTELPVVLV
                     DVMRGGPGLGNIAPGQGDYNQIVHGGGHGEYRAIVLAPGSVQESVDLTVLAFDLAEKY
                     RSIVVVLSDGSIGQMMEPCELPPMQPLKTKFPDWAVTGSEGRDRRFLSSINLDPAAQE
                     RMNFRVMRRWQEIEANEVRYKEYYMEDARFVVIGFGSTGRVALSAVRAARAEGIPVGL
                     IRPVTLNPFPTKAIAQAAQQAEVMLVVEMNNGQMLEDVMLAADGNAMVEFYGRMGGVF
                     MLPEEVLSEIRRIAAGGLSTEGHPRDRWLNRSAQKK"
     gene            1779..>2405
                     /locus_tag="PLW25_07680"
     CDS             1779..>2405
                     /locus_tag="PLW25_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOS54400.1"
                     /translation="MEMNSQVIEEQVYNRPDSFVDVSTHYCPGCTHGTAHRLIAEVID
                     EMGLKGKTIGVASVGCSVFAYNYFDVDFVQAPHGRAPAMATGVKRVLPERVVFTYQGD
                     GDLASIGTAEIVHAAARGENISVFFLNNTNYGMTGGQMAPTTLPGQKTTSSPRGRDVE
                     TQGYPVKASEMISTLDGASYVVRRSLHDPKSIRLAKKAIRTAFEVQIRG"
BASE COUNT          539 a          591 c          759 g          516 t
ORIGIN      
        1 gggatctctg aaaccatcac gaatgtgaat ggcggggcga ttgccctggg tcacccgctt
       61 ggctgctccg gcgcgcgcat cctcaccacg ctgctgcatg aaatgaaacg gcgcgctcct
      121 tctgcgccga ggccgtatta cgggatggct atgctatgtg ttggggtggg acagggtgaa
      181 gccaccattg tggagtgggc ggcatgagta gcatatatgc caggtcttta ttcaagagag
      241 aaagaatccg gttgtcgggc aggagttcta ctcctgcttt tttaactgca atggacagga
      301 ggagagacac tgttcaaaaa ccgtggtttt gtggataata ttaatccacc ggtgagaata
      361 ccggtgacct atccccaagc tggggaatta aaggaggtat aaatgccccc aaagggtcgg
      421 gttgtcgtca gcgaaatata ttgcaaaggc tgtgaacttt gtgtgaatgc ctgtccgcaa
      481 agcgtacttg ggttggacca cgaacatctc actgccaaag ggtaccaccc cgctgtgttg
      541 atcgcagagg gctgcaccgg ctgcggcatt tgcgcggtgg tgtgccctga tgcagccata
      601 acagtctatc gaattcgtac cggtgcaacc cgggaactgg attaggaggc gggcgatggc
      661 aaaagagtta atgaaaggca atgaagctgt cgccgaggct gcgatccgcg ccggggtgac
      721 cgcgtatttt ggctacccga ttacgccgca gaccgaatta ctggagtata tgtcaaggcg
      781 catgccggaa ctggggcggg tatttgtgca ggcggaaagt gagcttgccg ccattaacat
      841 ggtctatggc gcggcctgca cagggcggcg cgtgatgagt tcttcatcca gccccggcgt
      901 gagcctgatg atggagggag tttcttacat cgccggcacc gagctgccgg tggtgctggt
      961 ggatgtgatg cgcggtgggc ctgggcttgg caacatcgcc cccgggcagg gtgattataa
     1021 ccagatcgtg catgggggtg gtcatggtga ataccgcgcc attgtgttgg ctcccggcag
     1081 tgtgcaggaa tccgtagacc tcaccgtact ggcttttgac ctggcggaaa aataccgctc
     1141 gatcgtggtg gtgctttcag atggttcaat tgggcagatg atggaaccct gcgaactacc
     1201 acccatgcag ccacttaaaa ccaaattccc tgattgggct gtaactggct cagaaggacg
     1261 ggacaggcgc tttttgagtt cgattaatct cgacccggcg gcgcaggaaa ggatgaactt
     1321 cagggttatg cgccgctggc aggaaatcga agccaacgaa gtacgctaca aagaatacta
     1381 tatggaagac gccaggttcg tagtcatcgg gtttggttca accgggcggg tggcgctttc
     1441 agccgtcagg gcagcgcgcg ccgaaggaat cccggtggga ctgattcgac cagtgaccct
     1501 caatcccttc cccacaaaag ccattgccca ggccgcgcag caggcggagg tgatgctggt
     1561 ggtggaaatg aacaacgggc agatgctcga ggatgtgatg ctggcagcgg atggaaacgc
     1621 catggtggaa ttctatggac gcatgggcgg tgttttcatg ctcccagagg aagtgctgag
     1681 tgagatccgc cgcatcgcgg ctggcgggct ttccactgag gggcatccac gtgaccgctg
     1741 gttgaaccgc agcgcgcaga agaagtgagg agttgaacat ggaaatgaat agtcaggtga
     1801 tagaagagca ggtttacaac cgaccagatt cttttgtgga tgtgagcacg cattattgtc
     1861 ccggctgcac gcatggtaca gcgcatcgct tgatcgccga agtgattgat gaaatgggat
     1921 taaaagggaa gaccattggt gtggcatcgg ttggctgttc ggtttttgcc tataactact
     1981 ttgatgtgga ttttgtccag gcgccgcacg ggcgcgcgcc agcaatggca accggggtga
     2041 aacgcgtttt accagagcgc gtggtgttca cctaccaggg agatggcgac ctggcctcca
     2101 ttggcacggc ggagatcgtg cacgccgctg cccgagggga gaatatctcc gtgttctttc
     2161 tgaacaacac caattatggc atgaccggag gtcaaatggc gcccaccacc cttccgggtc
     2221 aaaaaacgac ctcttctcca cgggggcgcg atgtggaaac acagggctac ccagtcaaag
     2281 catctgaaat gatctccacg ctggatgggg cttcttacgt ggtgcggcgt tcgctgcacg
     2341 acccaaagag tatccgcctg gcaaaaaagg ctatccgcac cgcgtttgaa gtacaaattc
     2401 gcggg
//
LOCUS       DAOSPM010000100         2278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_47315_length_2278_cov_16.156095, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000100 DAOSPM010000000
VERSION     DAOSPM010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47315_length_2278_cov_16.156095"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..873
                     /locus_tag="PLW25_07685"
     CDS             196..873
                     /locus_tag="PLW25_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54401.1"
                     /translation="MPPTSPAEQIRSVLQVLKRHYGAPVWRDPLPAIDELVSTILSQN
                     TNDSNRDRAFNTLRQTFADWDAVRTAPDEEIIAAVRVAGLANQKGPRIKAVLNQIHEE
                     VGSLDLSFLAEMPLEEARAWLLRFKGVGRKTAAIVLQFALGRPAFPVDTHIYRVTGRL
                     GVRPAAMNVEKAHLLMEELIDPADFYAGHLNLIRLGRETCHPRKPDCPRCPVRELCLY
                     PDKTVEP"
     gene            1088..1642
                     /locus_tag="PLW25_07690"
     CDS             1088..1642
                     /locus_tag="PLW25_07690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOS54402.1"
                     /translation="MTEMWLVRHGQTDWNLRGIYQGQSDIPLNETGINQARALAASLS
                     GMRFEAIYSSDLGRALHTASFIASTLGLPVIADPRLREINQGVWEGQTIEAVREKYHP
                     DFSPNGKYISTPRAEGAESLEQVIRRMVAAANEYHARHNSGRLLLSTHGLSAAALYAV
                     ARGIPLVDVARYIPENTQPLLIQL"
     gene            1785..>2278
                     /locus_tag="PLW25_07695"
     CDS             1785..>2278
                     /locus_tag="PLW25_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54403.1"
                     /translation="MNLTATRSAIHHPLQTLKSVRVRSWRVYSAIIVLALIAFEAVNY
                     STTAYALRDLLGDLQFLGIRWATLMALAFCSLDFAGIARLVTQKSKLQASRESWFLFG
                     AWLIAGTFNAALTWWGVTIAITNHPLRSAEVVSTHNLTAVVPLVVAILVWVIRILIIG
                     SLTSS"
BASE COUNT          499 a          681 c          594 g          504 t
ORIGIN      
        1 cggaaaaccc ctgcgcaatg aagacatcca gaaaaccgcc gccaacattc tcaaacagtg
       61 caatgtacca aaggaagaag agctgcagca cctgcaacgc gcgcgctggg gcctggtgca
      121 gtatctcaag cacagcacgg gcactcaccc agaacaacct gccgtgttat aaggtaaagg
      181 gaaaacatca atcgaatgcc gcccacgtcg ccagcagaac aaatccgctc cgtgcttcag
      241 gtgctcaaac gccattacgg cgcgccggtt tggcgcgatc ctttgccagc gatcgatgag
      301 cttgtctcca cgattctttc gcagaacacg aatgatagta atcgcgatcg ggcgttcaat
      361 accctgcgtc agacctttgc cgattgggac gcggtgcgta ccgccccaga cgaggagatc
      421 attgccgcgg tacgcgttgc cggtttggcc aaccagaagg gcccgcgcat caaggcggtt
      481 ctgaaccaga tacatgagga ggtagggtca ctcgaccttt ccttcctggc ggagatgccg
      541 ctggaagaag cccgcgcctg gctgctgcgt ttcaaagggg tggggcgcaa aacagctgcc
      601 attgtgctgc agttcgcgct gggacgcccc gccttccccg tggatacgca catttaccgc
      661 gtcaccggcc gcctgggggt gagacccgcc gccatgaacg tggaaaaagc gcacctgttg
      721 atggaagaat tgatcgatcc tgccgatttt tacgccgggc acctgaacct gatccgactg
      781 ggtcgtgaaa cctgccaccc ccgcaaaccc gattgtccgc gctgcccggt gagagaatta
      841 tgcctttacc ccgataagac cgtagagccg taaaccccgg gcgcggtttt tacctcactt
      901 tgtcctttcc tcctttgtaa aaatacggtt cacaacacat tcttatcgaa atacaccttc
      961 gctcctgccg ttcttcttcc ttcaccagag gataccaaag ccaggttatt ttgttctggc
     1021 gcacgtccaa atgaactggc gaaaaacgat ccaacccgcg atttttcatc agttataatc
     1081 caatcttatg acggaaatgt ggctggtcag gcacgggcaa acggattgga acctgcgcgg
     1141 catctaccag ggtcaaagtg atatcccgct gaatgagacg ggtatcaacc aggcacgcgc
     1201 gctggctgct tcgctcagcg gcatgcgctt tgaagccatc tactccagcg acctggggcg
     1261 cgcgctgcat accgcgtctt tcatcgcctc tacgctgggg ttgccggtaa tcgctgaccc
     1321 gcgcctgcgt gagatcaacc agggcgtgtg ggaagggcag accatcgagg cggtgcgcga
     1381 gaagtaccat ccagatttca gcccaaatgg taaatacatt tccaccccac gcgcggaggg
     1441 agccgaatct ctcgagcagg tgatcaggcg tatggtcgcg gcggccaatg agtaccacgc
     1501 ccgccataac agtggtcgtc tgctgctctc cacgcatggg ctatccgccg cagcgttgta
     1561 cgctgttgcc agaggaatcc cgctggtgga tgtggcgcgc tatataccag aaaacacaca
     1621 gccgctgtta atccagctgt agaacccgtt ggaattaccg gatttcagcg cttacatttc
     1681 tgaaaaccaa tagaacgaat ttcctaaatc tcttgactta atagaatact tgttctataa
     1741 tatgactgtt ggtgttgatc atttcgtttt caaggagaac agtcatgaac ctaaccgcca
     1801 cccgttccgc tatccatcac cctctgcaga cgcttaagag cgtccgcgtg cgctcctgga
     1861 gggtatacag cgcgatcatc gtgctcgcgc ttattgcttt cgaagccgtt aattacagca
     1921 ccaccgccta cgccctgcgt gacttgctgg gtgacctgca gttcttaggg atacgttggg
     1981 cgaccctgat ggcgctggct ttctgcagcc tcgatttcgc cggtatcgcc cgcctggtaa
     2041 cccagaagtc caaactccag gcttcacgag aatcctggtt tctgtttggt gcctggctca
     2101 tcgctggcac ttttaatgcc gcgctcacct ggtggggtgt caccattgcc atcaccaatc
     2161 atccgctgcg cagcgcagaa gtggtaagca cacacaacct taccgccgtt gtaccgctcg
     2221 ttgtggccat cctggtttgg gtcattcgca tcctcatcat cgggtcgctc acctcttc
//
LOCUS       DAOSPM010000101         2206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_49425_length_2206_cov_14.899582, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000101 DAOSPM010000000
VERSION     DAOSPM010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49425_length_2206_cov_14.899582"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PLW25_07700"
     CDS             complement(<1..539)
                     /locus_tag="PLW25_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54404.1"
                     /translation="MTDTSTQYDAIFVGNYTKDTIITPEGTRYADGGGMNYAAHAASA
                     LGLKAAVVTRLAKEDEHVVNGIRAHGIDCYPVFSPSSTLMTLEYKTSDVDKRSLYVKA
                     TAGTIRAADLAGLAARAIVVSPSLRGEVEADFFETQRKRQGVLLSADVQGFVRVLRGE
                     SLVYEPWPDMERVLRQLDVL"
     gene            complement(558..1553)
                     /locus_tag="PLW25_07705"
     CDS             complement(558..1553)
                     /locus_tag="PLW25_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOS54405.1"
                     /translation="MSFTLILLVGLAVIFNFLNGVHDSANIVATAISSRAMAPRTALI
                     VVAIAEFCGPFIFGVAVANTIGNGVVASDQINSQVIIAALISAIVWNILTWVLGIPSS
                     SMHALIGGLIGAVVAGAGFMAIKLDGVIKVLISLFASPIIGFFAGILIANLIFLLSWK
                     SSPKINGVFKKLQIITSLGLALSHGTNDAQKTMGIITLGLLISGMISSFHVPFWVIFV
                     CALSMAIGISVGGWQLIRTLGSRFYKIRPVHGFASQFASTAVILSASLLGAPVSTTQV
                     VSTAIMGVGAAERVSKVRWGVAINIGFAWLYTIPLTALLAAGIYAFMKFILPMIT"
     gene            complement(1560..2180)
                     /locus_tag="PLW25_07710"
     CDS             complement(1560..2180)
                     /locus_tag="PLW25_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOS54406.1"
                     /translation="MLKIFKKRRNVFIHLMHEQATLTLQGMDLLKDYLSYPAKQLADE
                     LTMKEKEADECRRILIDELNRTFITPFDREDIFSLSRAIDDIIDYAYSTMTEIVILKV
                     DPTEFMVRICSLLRDAAYELMMAVEQLQDHPAVAAEHAQRAKALENRVETVYREALAD
                     MFQKADDVKGLVRIMKLREVYRHLSNAADRGDEAANVIADIIVKTT"
BASE COUNT          552 a          609 c          544 g          501 t
ORIGIN      
        1 agaacatcca gctggcgcag tacgcgttcc atatccggcc agggttcgta caccagcgac
       61 tcaccgcgca acacccgcac aaacccctgc acatctgctg acagcaacac accctgccgt
      121 ttccgctgtg tttcaaaaaa gtccgcttcc acctcaccgc gcaggctggg gctcaccacg
      181 atggcgcggg ctgccaaccc cgccagatca gctgcgcgga tcgttcctgc agttgccttc
      241 acataaaggc tgcgtttatc cacgtcactg gtcttgtact ccagcgtcat cagggtggaa
      301 gatggagaaa agaccgggta acaatcaata ccgtgagccc gtattccgtt gaccacatgc
      361 tcatcttctt ttgccaggcg ggttaccacc gcagccttca gccccagggc gctggcagcg
      421 tgagcggcat agttcatccc gccgccatcc gcgtaacgcg tgccttcagg cgtgataatg
      481 gtatctttcg tgtagttgcc cacaaaaatc gcatcgtact gtgtacttgt gtctgtcatc
      541 tgtacctctt aataaagtta ggtgatcatg gggaggataa atttcataaa agcataaata
      601 ccagccgcca ggagcgcggt caaagggatg gtatacaacc aggcaaaacc gatattgatg
      661 gccactcccc aacgcacctt gctcacccgt tccgcggcgc ccacccccat aatggctgtg
      721 ctgaccacct gggtggtgct caccggggcg cccaatagag aggcggagag gatgaccgcg
      781 gtagaagcaa actgagaggc gaacccgtgc acagggcgta tcttatagaa cctggacccc
      841 agggttcgga tcaattgcca tcctcccacg gagatgccta ttgccatcga gagggcgcaa
      901 acaaaaatca cccaaaacgg cacgtgaaaa cttgagatca tgccgctgat gagcaaacct
      961 aaagtgatga tgcccatggt tttctgcgcg tcattggtgc catggctcaa cgccaacccc
     1021 aacgaggtga tgatttgcag ctttttgaaa accccattga tttttgggga actcttccac
     1081 gaaaggagaa aaatcaggtt ggcgatcaaa atccccgcaa aaaaaccaat gatcggagag
     1141 gcgaatagcg agatcaatac cttgatcaca ccatccagtt tgatcgccat aaagcctgcg
     1201 cccgccacca cagcgccgat caagccgcca attaacgcgt gcatggagct gctggggata
     1261 cccagtaccc aggttaagat attccacacg atcgcgctga ttaatgccgc aatgatcacc
     1321 tgcgaattga tctgatcgga tgcgacaaca ccgttcccga tcgtattggc aaccgcgacg
     1381 ccgaaaatga aagggccgca gaattctgcg atcgccacca caataagcgc cgtccgtgga
     1441 gccatggccc gcgatgagat ggctgttgcc actatgtttg cagagtcgtg aactccatta
     1501 agaaaattaa aaattacggc taagccaact aaaagtataa gggtgaagga catgtttttt
     1561 tatgtcgtct taacgatgat gtctgcgatt acatttgcgg cttcgtcacc gcggtcagct
     1621 gcgttgctaa ggtggcggta aacttcgcga agcttcatta tccttaccag ccctttaaca
     1681 tcgtccgcct tctggaacat atctgctaac gcttcacggt acaccgtttc gacacggttt
     1741 tcgagcgctt tggcgcgctg cgcgtgttca gctgccactg ccgggtgatc ctgtaattgt
     1801 tccacagcca tcatcagctc gtacgcggca tcacgcagca aggagcaaat gcgcaccatg
     1861 aattccgtgg ggtcaacctt gaggatgaca atttctgtca tggtactgta tgcataatcg
     1921 atgatgtcat caatggcacg cgaaagcgaa aaaatatctt ctcgatcaaa gggggtgata
     1981 aatgtgcggt tcaattcatc aataagaata cgccggcatt catccgcctc tttttctttc
     2041 attgtcagct cgtctgccag ttgtttggcc gggtaagaga ggtaatcctt cagcaggtcc
     2101 atcccctgca gcgtgagggt ggcctgctcg tgcatcaggt gaataaaaac gttacggcgt
     2161 ttcttaaaga tttttaacac tgatcctcct ggagtaattc agattt
//
LOCUS       DAOSPM010000102         2092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_53161_length_2092_cov_14.807069, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000102 DAOSPM010000000
VERSION     DAOSPM010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53161_length_2092_cov_14.807069"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLW25_07715"
     CDS             complement(<1..508)
                     /locus_tag="PLW25_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54407.1"
                     /translation="MTMYDLKSLNLPKLYGGVLKFFSAVTSNKLTRSLLVGSLLENGG
                     IPKLREMKFIEEPTNFPLVIPSERAWEPLELPQHIPSLSRPSRERLPFNMIRDFTAAY
                     LEGVVTPAQVAEEVIASIAASQQTTPPLNAFIASSAEDIRQQARDSTERLKNGQARSL
                     LEGVPLAIK"
     gene            complement(847..>2092)
                     /locus_tag="PLW25_07720"
     CDS             complement(847..>2092)
                     /locus_tag="PLW25_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS54408.1"
                     /translation="FYQRVLQGLRAYAGNVRAFSRNATLYLLSTILSSSAFGVFRLLF
                     NFYVLSLGYDQALVGNLVAASSMTALVAAIPMGYLADLLGRKLSLIGGGLVMVVAICG
                     MLLFPSPTIFILMNILMGLGQSISGVTMGPFLMENSGEKERTYLFSFSSGISMAASSV
                     GNWIGGLLPTWLAVYAGGDATSASAYRLALTIVAVAASTAVVPLFLMTIQRGKVVERS
                     LFAPFTYFKSHPALLGKLVLPMLVTSIGAGLVMPFMNVFFRQVHGQSDSAIGTLFAFG
                     SLAMGVGLLIAPPLAEKYGKIQFVVITQALSIPFLALLGFSPLYWAAAAAYYIRVALM
                     NMSGPVYQTFVLEKVEPSARATVASLVSMASNFGWAFSPTISGYIQVKHGFAPAFMLT
                     MILYMLSISLYWGFFWTRARKT"
BASE COUNT          542 a          580 c          560 g          410 t
ORIGIN      
        1 ctttgatcgc cagcggcacg ccctccagca ggctgcgcgc ctgaccgttc ttaagccttt
       61 cggttgaatc ccgcgcctgc tggcggatat cttcagctga actggcaatg aaagcattca
      121 gcggcggggt ggtctgctgg ctggcagcaa tagacgcaat cacctcctcc gccacctgcg
      181 caggggttac cacgccctcc aggtaggccg ctgtgaaatc gcgaatcata ttgaacggca
      241 accgttcacg agaggggcgg ctcagcgagg ggatgtgctg aggcagttcc aagggttccc
      301 aagcgcgctc cgagggaata accagcggga aattggttgg ctcttcgatg aatttcatct
      361 cgcgtaattt ggggattcca ccattctcaa gcaggctgcc aaccaacagc gagcgggtca
      421 atttgttgct ggtgacggcg gaaaaaaact tcaacacgcc gccatatagt ttgggcaggt
      481 tcagtgactt aaggtcgtac atggtcattt ttctcccttt ttggcttctt ttttaagaaa
      541 acaggcaact gcgcgttgga tacctcgttt atgataaacc attatatacg caaagcaggt
      601 gaactgccca aagcactgga ctacctcacc tcacctagcc gccccgcccg cctcgtaacc
      661 tcaatcatcc cctcgggtgg gagtggtttt ttcgtgagag gtccttgcgc ttataccgaa
      721 aaaacctcgt taaacgggca aaagagtctc agagaaacgc cgcctgaaag cctgcttacg
      781 gcataacccg ggtttgacct cgttaaaaag ggtttttctc aggttatttg acagccgcgc
      841 gcagggttat gttttcctgg cgcgagtcca gaaaaatccc caataaagcg aaatggaaag
      901 catatacagg atcatcgtga gcataaatgc cggcgcaaag ccgtgcttaa cctggatata
      961 accgctgata gtggggctaa aagcccaacc aaaattgctg gccatgctca ccaggctggc
     1021 gaccgtggcg cgcgccgagg gctcgacttt ttccaggaca aaggtctggt acaccggtcc
     1081 gctcatattc attaatgcca cacgaatata ataggcggca gcagcggccc aatacagcgg
     1141 ggaaaaaccc agcaaagcca ggaacgggat tgagagcgct tgagtaatga ccacaaactg
     1201 gatctttcca tatttctccg ccaggggggg cgcgatgagc agccccacgc ccatggcgag
     1261 cgacccgaaa gcaaacaacg taccgatggc gctgtccgat tgaccgtgca cctggcggaa
     1321 gaaaacgttc ataaatggca tgaccagacc agcgccaatg gatgtcacca gcatcggcag
     1381 aaccagtttg cccaacaacg ccggatggct cttaaagtaa gtaaaggggg caaacagcga
     1441 acgttccacc actttgccgc gctggatggt catcaaaaaa agcggcacca ccgcggtgct
     1501 ggcagccaca gccacaatgg taagtgcaag ccggtaagcg ctggcgctgg tggcgtctcc
     1561 tccggcgtat actgccagcc aggtcggcag taacccacca atccagttgc ccactgaaga
     1621 ggcggccatt gaaataccgg aactgaagct aaaaaggtag gtgcgctctt tttcaccgct
     1681 gttttccatc aggaatgggc ccatcgtcac accggagatg ctctggccca gccccatcaa
     1741 aatattcatt aatatgaaga tagtgggtga cgggaataaa agcataccgc agatggcaac
     1801 caccattacc aacccgccac caatcaacga aagcttgcgc cccagcaggt cagccaggta
     1861 acccatgggg atggccgcga cgagggcggt catactgctg gcggccacca ggttgccgac
     1921 cagcgcctgg tcataaccca ggctgagaac gtaaaaattg aacaacaaac ggaacacacc
     1981 gaacgccgat gagctgagga tggtgcttaa caggtagagt gttgcattgc gcgagaatgc
     2041 gcgcacattg ccagcgtaag ctcgcaaacc ttgaagcaca cgttgataga ac
//
LOCUS       DAOSPM010000103         2021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_55761_length_2021_cov_8.730926, whole genome shotgun sequence.
ACCESSION   DAOSPM010000103 DAOSPM010000000
VERSION     DAOSPM010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55761_length_2021_cov_8.730926"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PLW25_07725"
     CDS             <1..545
                     /locus_tag="PLW25_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS54409.1"
                     /translation="IYYQRQGNLEKALEHLRIAQESNPSAAIWILEGGRTLAAMGVLE
                     NALTEFSLAVTVDEKNADLWRALAEFCILHRYQLEDVGLPAARQALALEPDNPVMMDL
                     LGTVYLQLADLDSAERFFLRALARDPEQAAILIHLGQLNLIREEKEIAFDYLRRAEAS
                     ARDWRLRDLANRLLRENGAQ"
     gene            585..1253
                     /locus_tag="PLW25_07730"
     CDS             585..1253
                     /locus_tag="PLW25_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54410.1"
                     /translation="MNKPTYFLFRFFLFCCALTLTGCEKIGIHFPPFGAVPPGELLFE
                     DDFSHPPNGWGSIETRGGEIEFNYDGMMISIFLPNFMYWSVNGKEFSDVKVKVDAVLA
                     DGPQDDTFGVICRFLDDRNFYGFVLSHDGYYGVFKYVDGKMKVLSNEGNLGFSEAIRT
                     GGVVNHLEATCERNVLRLRANETLLTEVMDDQFESGKVGLIAGAYSEPGVRVFFDNFK
                     VFQP"
     gene            1256..>2021
                     /locus_tag="PLW25_07735"
     CDS             1256..>2021
                     /locus_tag="PLW25_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS54411.1"
                     /translation="MKIFFDTVGCRLNQAEIEHLASEFRSAGHTIIDTAEGADLVVVN
                     TCSVTAAAASDSRQKVRQAQHAGASNILVTGCLATLEPSEMARLPGVKQVVSNQGKPQ
                     IPGLVLGQLADFDLEPLARQPLPGARKRTRAFIKVQDGCNNHCTFCVTRIARGEARST
                     PREEVLREVNRALHGGSREVVLTGVHLGWWGRDLSHEETLPDLVGYLLEKTAIERLRL
                     SSTEPWDLDDRFFDLWRDPRMCRHLHLPLQSGSAEVL"
BASE COUNT          480 a          470 c          593 g          478 t
ORIGIN      
        1 ggatctatta ccagcgccag ggcaacctgg aaaaagcgct ggagcacctg cgaatagccc
       61 aagaatcgaa cccgtcggct gccatatgga ttctcgaagg cggaagaacc ctggcggcga
      121 tgggggtcct ggaaaatgcg ctgacagaat tttctcttgc tgttaccgtg gatgagaaga
      181 atgcggattt atggcgagcg ttggcggagt tctgcatttt gcatcgttat cagttagaag
      241 atgttgggtt gccggccgct cggcaggcgc tggccctgga accggataac ccggtaatga
      301 tggacttgct gggaaccgtc tacctgcaat tggctgacct ggatagcgcg gaacgttttt
      361 tcctgcgagc cctggcgcgg gaccctgaac aagcagccat tctgattcac ctgggacagc
      421 tcaacttgat aagagaagag aaagagatag cctttgacta tctacgccgt gctgaagcat
      481 ctgcccgcga ttggcgcctg cgcgatctgg caaaccgcct gttgagagaa aatggcgcgc
      541 agtaaatggg gaaggtataa tattgcagtc aggaacaggc aaaagtgaac aaaccgactt
      601 actttttatt ccgttttttt ctcttttgct gcgcattgac cctgacaggc tgcgagaaga
      661 tcggcattca ctttcctcca ttcggggcgg tacccccggg ggaactgtta ttcgaagatg
      721 atttttccca cccgcccaat ggctggggat cgattgaaac gagaggcggg gagatcgaat
      781 ttaactatga tgggatgatg atttccatct tcctcccgaa ttttatgtac tggtctgtga
      841 atggaaagga attcagcgat gtgaaagtta aggtggatgc tgtgctggcg gatggtccac
      901 aggatgatac ctttggcgtc atctgccgct ttctggatga ccgtaacttt tacgggttcg
      961 tattgagcca tgacggctat tacggggttt ttaaatatgt ggatggaaaa atgaaagtgc
     1021 tatcaaatga agggaatctg gggttcagcg aagccatccg cacgggtggc gtggtcaatc
     1081 acctggaggc gacctgcgag cgaaatgttc ttcgtttaag agcgaacgaa acacttctaa
     1141 ctgaggtgat ggatgatcag tttgaatccg gtaaagtggg gcttatcgcc ggcgcttata
     1201 gcgagcctgg cgtgagggtg ttttttgata atttcaaggt attccagcca taacaatgaa
     1261 aatatttttt gataccgtcg gttgcaggct caatcaggca gaaattgagc acctggcgag
     1321 tgaattcagg tcagccgggc acactatcat cgacactgct gagggggcag acctggttgt
     1381 ggtaaacaca tgctcggtca ctgcggccgc ggcttctgat tcccgccaaa aagtaagaca
     1441 ggcacagcac gccggcgcct cgaacatcct ggtaaccggc tgcctggcca ccttagagcc
     1501 gtctgaaatg gcaaggttgc cgggcgtcaa gcaggtggtt tccaaccaag gtaagccgca
     1561 aataccgggg ctggtattgg ggcaactggc ggattttgac cttgaaccac tagccaggca
     1621 gcctttgcct ggagcgcgaa aacgcaccag ggctttcata aaagtgcagg atggatgtaa
     1681 taatcattgc actttttgcg tgacgcgcat cgctcgcggt gaagcgcgca gtacacccag
     1741 agaagaagta ttgcgtgaag tcaaccgtgc cctccacgga ggaagccggg aggtggtatt
     1801 gacgggtgtg cacctgggct ggtggggtcg agatttaagc catgaagaaa ccttacctga
     1861 ccttgtgggt tacctgttgg aaaaaactgc gatagagcgg ctgcggttgt cttcaactga
     1921 accatgggac ctggacgacc gcttttttga tctctggcga gatccacgca tgtgccgcca
     1981 cctgcatcta cccttgcagt caggttcagc ggaagtgctc a
//
LOCUS       DAOSPM010000104         1995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_56717_length_1995_cov_10.632990, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000104 DAOSPM010000000
VERSION     DAOSPM010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56717_length_1995_cov_10.632990"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="PLW25_07740"
     CDS             <1..1216
                     /locus_tag="PLW25_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54412.1"
                     /translation="DERQVLWLWGLLSILPQSLLASYSGMETSLLVFIIGWLLYQVSR
                     GRDHWLMMAALAILPWVRPDAIAYALLILFWDCVKRKRVLWAGLTAVVIGVSSLLIFN
                     SLYFGTLLQQSINAKLLMRHPFTISRFADNLFTVFVGNAGGAFSPYRAEIFGRAGILF
                     GLLVLLAMGLYLGQVWSDRAPRITGLALASITWLVPVAYAFGGVLYQWYTWPPAIFGS
                     VFLLALIVEWSTGTGKWRQVVRWGCVLLMLAGLNAQMVFSYVWGTKEFAYRGGIGVWL
                     KEHAQAGDKMLLEPAGYIPYYSGLYTYDEVGLVSPQVVNYRLAYEERWWPEFVMDYRP
                     DWLVQREHIKHDKTYQGYTLNGEELEWLRDNYRLVAWFSYQPEDYTSSHFLASLLSLA
                     EADDYYIFKRIE"
     gene            complement(1301..1465)
                     /locus_tag="PLW25_07745"
     CDS             complement(1301..1465)
                     /locus_tag="PLW25_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54413.1"
                     /translation="MKLTPPKNITWVIALVVGVLGIIGHLAGFFGDFAFWLVVIGFVI
                     LVLSTLLKDL"
     gene            complement(1553..>1995)
                     /locus_tag="PLW25_07750"
     CDS             complement(1553..>1995)
                     /locus_tag="PLW25_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54414.1"
                     /translation="PGNEETMKNITVSTAIVLLLCFLLITGCAQKQAVTYLSADESAQ
                     VATYTDVAAQNIINAIAANDYNLFISDFDEQMRHALPEEQFATIVKMYVKNGQAESIS
                     LLNMEDRGDFIGANYGVTYSNAALTMLIVVAKSDPSLVSGLWFK"
BASE COUNT          441 a          450 c          553 g          551 t
ORIGIN      
        1 cgatgagcgg caggttttat ggttgtgggg gctgttatcc atcctcccgc agtctctgct
       61 ggcgagctat tcagggatgg aaacttcact gttggttttt atcatcggct ggttgcttta
      121 ccaggtatcc agggggcgcg accattggct gatgatggct gccttagcga tcctgccctg
      181 ggtgcgcccc gacgccattg cttacgcttt actgattctc ttttgggatt gcgtcaagcg
      241 aaagcgcgtg ctttgggctg gattgaccgc agtggtaatc ggtgtgtcca gtctgcttat
      301 ctttaattcc ctttattttg gcacgttgct gcagcagtcc atcaatgcca agctgctgat
      361 gcggcatccg ttcacgataa gccgtttcgc ggacaacctg ttcaccgttt tcgtcggcaa
      421 cgccggtggc gcgttttctc cttacagggc tgaaattttc ggcagggcag gcattctgtt
      481 tggattgctc gttttgctgg cgatggggct ttacctcggg caagtatgga gtgatagagc
      541 gccgcgtatc accggtttgg cgctcgccag tatcacctgg ctggtgccgg tggcttacgc
      601 cttcggaggg gtgctctacc agtggtacac ctggccgcct gccattttcg gttcggtatt
      661 tttactggcg ttgatcgtgg agtggtcaac agggactggg aagtggaggc aggtggtacg
      721 ttggggatgc gtgctgctga tgttggcagg gctaaatgcg caaatggtgt tctcgtatgt
      781 ttggggaacc aaagaattcg cttatcgggg gggaattggc gtctggctga aagaacacgc
      841 ccaggcaggt gataagatgt tgttagagcc tgccggttac atcccttatt actcagggtt
      901 atatacctat gatgaggtcg ggttggtgtc accgcaagtg gtgaattacc gcctggctta
      961 tgaggagcgc tggtggccag aatttgtgat ggattaccgc cctgactggt tggtgcagcg
     1021 agagcatatt aaacacgata aaacgtacca gggctatacc ttgaatggag aagagctgga
     1081 atggttaagg gataattacc gcctggtcgc ctggttctct taccaaccgg aagactacac
     1141 cagcagccac ttcctggcga gtttgctttc gctggctgaa gcggatgatt attacatttt
     1201 caagcggatt gaatagaaaa atccggcgac agccggattt ttttgaggta attctcttca
     1261 ctgaattgaa aatcccgggt ttccccgggg cacggcaaac ttacaagtct ttgagcagtg
     1321 tagagaggac gaggattaca aagccgatca caaccagcca aaaagcgaag tcgccaaaga
     1381 aacccgccag atgaccaatg ataccgagaa caccaacgac cagggcgatg acccaggtta
     1441 tatttttagg gggggttagt ttcatagtag gtatactcct ttttattcat ccagcgtaaa
     1501 gaattaccat gctgatatta taaacaacat ttcggtcatt ttcatttcac atctatttga
     1561 accataatcc gcttaccagc gaaggatctg atttggcgac gacgatcaac atggtgagcg
     1621 cagcgttcga gtaggtgaca ccataattgg ccccgatgaa atctcctctg tcttccatgt
     1681 ttaataaaga aatgctctct gcctgaccgt ttttgacata cattttcaca atggtggcaa
     1741 attgctcttc gggcagcgca tggcgcattt gttcatcgaa atcgctgatg aacaggttgt
     1801 aatcgttcgc ggcgatggca ttaatgatgt tctgggcggc aacatcggtg taggttgcca
     1861 cctgagcgct ttcatctgcc gataaataag tgacagcctg tttttgagcg cacccggtaa
     1921 ttaaaagaaa gcaaaggagc agcactattg cggtagatac ggtgatgttt ttcattgttt
     1981 cctcgtttcc tggtc
//
LOCUS       DAOSPM010000105         1912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_60233_length_1912_cov_19.639742, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000105 DAOSPM010000000
VERSION     DAOSPM010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60233_length_1912_cov_19.639742"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PLW25_07755"
     CDS             <1..1110
                     /locus_tag="PLW25_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54415.1"
                     /translation="GEEQELEQERNRLANAEVLTANTQEVLNILDEGSPETSSASELL
                     GQASKLLAALARVDSQQVGLVDQLEEASTTLTELSRDLRAYLDGIEFNPRRLEQVEER
                     LELIHQLQRKYGGTVEAVNEFGLSARRELEKVQHAGERIEELEQEENAAILKTLSAGV
                     NLSRLRRQAAQRMGQEVESELNDLSLAGARFSVWFNELPELDGLMPQDALKVDLKESG
                     LDEVEFLIAPNPGEGLKPLVKIASGGETSRLMLALKNVLAQADHIPTLIFDEIDQGIG
                     GRVGSVVGEKMWRLARRHQVMCVTHLPQLAAFGDEHYKVSKSVIDQRTLTGVKRLGED
                     ERIEELALMNGSISLSSLGAARELLSRARERQAAA"
     gene            1128..>1912
                     /locus_tag="PLW25_07760"
     CDS             1128..>1912
                     /locus_tag="PLW25_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54416.1"
                     /translation="MDTPKLKILLLGPPEVTLEDRPVLIKRRLNRALLFYLAAQNNPV
                     TREEICNLFWPDAPEEIAKKNLREALSRLRTSIGFTDLIFSEGEQLFLNPKRVWVDSR
                     EMDRLITPLMSSSEMHRGSALPEWMVAQLKEGFALCRANRFMQGTTLNSAAGFENWME
                     LTNQAYRYSRIKIVDRLIDHYISGGNLEEALIWLGKGIDINPFDEDWNYLALICLRDT
                     GRIQEMIDYITYMEKLYQQQEEELPARFTAFKTDAIARKEIEN"
BASE COUNT          486 a          471 c          574 g          381 t
ORIGIN      
        1 ggcgaggaac aggaactgga acaggagcgc aaccgcctgg caaacgcgga ggtgcttacc
       61 gccaacaccc aggaggttct caacatcctg gatgaaggct cgcctgaaac ctcatccgca
      121 tcggagctgt tggggcaggc ttccaagctt ctcgcagcgc tggcgcgagt tgactcgcag
      181 caggttgggc ttgtcgacca gctggaagaa gccagcacaa cgcttactga actcagccgt
      241 gacctgcgcg cctacctcga tggaatcgag ttcaatccca ggcgactgga gcaggtggaa
      301 gagcgcctgg agttgatcca tcaattacag cgaaaatacg gcggcacggt cgaagcggtg
      361 aatgaatttg gcctctcagc caggcgtgaa ctggaaaaag tgcagcacgc aggcgaacgg
      421 atagaggaac tggagcaaga ggaaaacgcc gcgatcctta aaacactgag cgcgggcgta
      481 aacttgagcc gtttgcgcag gcaagccgcg caacggatgg gccaggaagt ggaatctgaa
      541 ctgaatgacc tcagcctggc gggtgcgcgc ttctcggttt ggttcaacga gctgccggag
      601 ctggatggac tgatgccgca ggacgcgctt aaggttgacc ttaaagagtc tggtctggat
      661 gaagtggagt ttctcatcgc tcctaacccc ggggagggat taaaaccgct ggtcaagatc
      721 gcttcgggtg gtgaaacttc gcgcttgatg ctggcgttga aaaatgtgct ggcacaggct
      781 gaccatattc ccacgctcat ttttgacgaa atcgaccagg gcattggcgg cagggtaggt
      841 tcggtggttg gtgaaaagat gtggcgcctg gccaggcgac accaggtgat gtgcgtaaca
      901 cacctgccgc agctggcagc ctttggcgat gaacattaca aggtgagcaa aagcgttatt
      961 gaccaacgca ccctgaccgg ggtgaagaga ctgggagaag atgaacgcat tgaagaactc
     1021 gcgttaatga acggcagcat cagtctatcc agtctgggcg ctgcccgcga gctgctctct
     1081 cgagcgcgtg aacggcaagc tgctgcataa aaacgcggaa tcgattcatg gataccccta
     1141 aattaaagat cttgctgctg ggaccgccgg aggtgacact ggaagacagg ccggtgctca
     1201 tcaagcgccg cctgaaccgg gcgctgcttt tctatctggc tgcgcaaaac aacccggtca
     1261 cacgtgaaga gatttgcaat ctgttctggc ctgatgcgcc agaagaaatc gccaaaaaga
     1321 accttcgaga agccttgagc cggctacgca ccagcattgg gtttaccgac ctgatttttt
     1381 cagaaggcga acaattgttt ttaaacccaa aacgcgtctg ggtggattcc cgagagatgg
     1441 acaggttgat cacgccgctg atgagcagtt cagaaatgca cagaggatcc gccctgcccg
     1501 agtggatggt cgctcagttg aaggagggtt tcgctctgtg ccgcgcaaac cgttttatgc
     1561 agggcacgac actcaacagc gcggctggat ttgagaactg gatggagttg accaatcagg
     1621 cttatcgata ttccaggatc aagatcgttg accgcctgat agatcattat atttccgggg
     1681 gaaacctgga ggaagcgtta atctggttgg gaaaaggaat tgatatcaac ccgtttgacg
     1741 aagattggaa ttacctggcg ctcatttgcc tgcgggatac cgggcgaatt caggaaatga
     1801 tcgattacat cacctacatg gaaaaacttt accagcagca agaggaagag ctgccggcgc
     1861 ggttcacggc ttttaaaacg gacgcgattg ccaggaaaga gattgaaaac ca
//
LOCUS       DAOSPM010000106         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2319
            NODE_65517_length_1801_cov_10.272050, whole genome shotgun
            sequence.
ACCESSION   DAOSPM010000106 DAOSPM010000000
VERSION     DAOSPM010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864386
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:20:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,554
            CDSs (total)                      :: 1,524
            Genes (coding)                    :: 1,520
            CDSs (with protein)               :: 1,520
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65517_length_1801_cov_10.272050"
                     /isolate="AMR_MDS_2319"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PLW25_07765"
     CDS             <1..881
                     /locus_tag="PLW25_07765"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS54417.1"
                     /translation="IADILPENQRQEGFGILRVVGNLAWIFGPTIGGFVAGKSFLALF
                     IIDAVISSLVALLFYLLIPETKPQNQPGEKPESLLVTFKGYFQVLRHGAFIAFMAAGM
                     LMGLVYQQMYNSLSVYLRDNHGIDPKGYGFLLTASAIVVILFQFSMTRWIKKKPAFKM
                     MALGTLFYMLGFSMFGFVGQYWLFVLAIMIITTGEMIVVPTSQALAVNFATEDMRGRY
                     MGVFGITWMLPATIGPGAAGLILDHFNPNLLWYIGGVLCAISALAYYGLHLKIGAQER
                     FQPAQDEPAAVEDPAV"
     gene            912..1643
                     /locus_tag="PLW25_07770"
     CDS             912..1643
                     /locus_tag="PLW25_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS54418.1"
                     /translation="MKSNNAIQFRSLYEGFDTPLTGLDCGFKCAPHNPRGIPFCCDLE
                     YTVPVAYRSEWRYLSGHTSLWRPWRGGLSASEKEAFADLQRQIPEHLCLLQCLGARSC
                     DRSYRTLSCRQFPFFPYLNSHARFIGLTYDWEFRHQCWVISNLRLVSDIYRKEFIHTF
                     DQLFQTWPEDMQCYADLCVETRAYYQAERRRIPLLHRDGSFCLISPCSERVQRVSAER
                     LPRFTPYIPAQSTPTPIKTQAKIDD"
BASE COUNT          357 a          508 c          482 g          454 t
ORIGIN      
        1 tgatcgcgga catcctgccc gaaaatcaac gccaggaagg ttttggtatt ctgcgcgtgg
       61 tcggtaacct cgcctggatt ttcggaccca ccattggcgg ttttgtcgcc ggtaaatcct
      121 tccttgctct ctttattatc gacgcggtca tcagcagcct ggtggcgttg ctgttctacc
      181 tgctcattcc tgaaaccaaa ccgcagaatc agccgggcga gaaaccagaa agcttgctgg
      241 tcacttttaa aggatatttc caggtgctgc gtcatggggc tttcatcgcc tttatggctg
      301 ccggcatgct gatggggctg gtttaccagc agatgtataa ttcgctctct gtttatctcc
      361 gcgacaacca cggcatcgac cccaaagggt atggtttttt gctcaccgcc agtgccatcg
      421 tggttatcct cttccagttc agcatgacgc gctggattaa aaagaaaccc gcctttaaga
      481 tgatggcgtt ggggaccctg ttttacatgc tggggttcag tatgttcggc tttgtcggtc
      541 agtactggtt gttcgttctg gcaatcatga tcatcaccac cggagagatg atcgtggtac
      601 ccaccagcca ggcgctggca gtgaacttcg ccacagaaga tatgcgcggg cgctatatgg
      661 gtgttttcgg tattacctgg atgctccccg ccactatcgg tccgggggcc gccgggctca
      721 tccttgatca tttcaacccg aacctgctgt ggtacatcgg cggagtgttg tgcgccattt
      781 cagcgctggc ttactacgga ttgcacctga agatcggcgc gcaagagcgc tttcagccgg
      841 ctcaggatga acccgcggcg gtagaagacc ccgcggtgta atacccggcg agaacgctca
      901 ggtttcagcc tatgaaaagc aataacgcta tccagtttcg cagcctgtat gaaggctttg
      961 atacccctct gaccgggttg gactgcggtt ttaagtgcgc ccctcacaac ccgcgcggca
     1021 tcccgttctg ctgcgatttg gaatacactg tgccggtggc ttaccgttca gagtggcgct
     1081 atctcagcgg ccacacttct ttatggcgcc cctggcgggg cggcctgagc gcgagtgaaa
     1141 aagaagcctt cgctgacctg cagcgccaga tccctgagca cctttgcctg ttgcaatgcc
     1201 tcggagcgcg atcttgcgat cgctcttacc gcacgctgag ctgccgccag ttcccctttt
     1261 tcccttacct taacagccat gctcgcttta tcggtctcac ctatgattgg gagttcaggc
     1321 accagtgttg ggtgatcagc aacctgcgcc tggtgagtga tatctacagg aaagagttca
     1381 tccacacctt tgaccagctt tttcagacct ggccggaaga tatgcagtgt tacgctgacc
     1441 tgtgcgttga aacccgtgcg tattatcaag cagagcgcag gcgcatcccg ctgctgcacc
     1501 gcgacgggtc tttctgcctg atcagccctt gcagtgaaag ggtgcagcgg gtgagcgctg
     1561 agcgcctgcc gcgtttcaca ccctacattc ctgctcaaag cacaccaacc ccgatcaaga
     1621 cacaggcgaa gattgacgat tgattttctg gtctcgaaag ggtatgatat ccttataagc
     1681 caggtgaact ggaggtgcca catgaaaaga agatgcctct tcgtcattgc gctgttgctg
     1741 agcatttctt tgagcgcttg caatctgccc atcaacgccg gtcattcggg tgcaatttcc
     1801 c
//
